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Florida Atlantic University

1. Oseni, Saheed Oluwasina. Enhancement of the Chemopreventive and Chemotherapeutic Effects of Genistein and Beta-lapachone in Human Prostate Cancer Cells by Pyroelectrically Generated Very Low Dose Ionizing Radiation.

Degree: MS, 2015, Florida Atlantic University

Summary: An estimated 220,800 new prostate cancer cases and 27,540 deaths are expected to occur in US men by the end of 2015. Despite the increased treatment modes for prostate cancer, there is still no definite cure, and prognosis remains, at best, cautiously optimistic. The explicit amalgamation of two or more cancer therapeutic modalities such as surgery, radiation, and chemotherapy, has been one of the main interests of clinical investigation for several decades. Genistein (GN) and Beta-lapachone (BL) are two of the most promising anticancer phytochemical compounds. However, the anticancer activities of BL have been correlated with the enzyme activity of NQO1. The aim of this study was to investigate the enhancing effects of VLDR derived from a portable pyroelectric crystal generator on the chemopreventive and/or chemotherapeutic effects of GN and BL in NQO1+ PC3 and NQO1± (deficient) LNCaP prostate cancer cells (PCa) in vitro. The combination treat ment-induced cytotoxicity was investigated via MTT and Trypan blue exclusion assays. Dicoumarol (an NQO1 inhibitor) was co-administered to assess the effect of VLDR on NQO1 modulation. Nitro-blue tetrazolium assay was used to assess the intracellular ROS levels. Fluorescence microscopy was also used to assess the mode of cell death. In this study, a novel quantitative modeling approach was employed to comparably assess the cytotoxic effects of specific drugs used alone or in combinations with VLDR and to predict the potential synergistic therapeutic combinations. The data suggests that VLDR induced a rise in ROS levels, followed by upregulation in NQO1 levels. Pharmacodynamic indices were developed to quantify and characterize the combination treatment as synergistic, additive or antagonistic per dose or time-interval. Synergism was found to be dose and time-interval dependent. The major mode of cell death by this combination therapeutic regimen was found to be via apoptosis . In conclusion, our results confirm that VLDR enhanced cytotoxicity effects of both drugs dose- and time-dependently.

2015

Degree granted: Thesis (M.S.) – Florida Atlantic University, 2015.

Collection: FAU

Advisors/Committee Members: Kumi-Diaka, James (Thesis advisor), Florida Atlantic University (Degree grantor), Charles E. Schmidt College of Science, Department of Biological Sciences.

Subjects/Keywords: Apoptosis  – Molecular aspects; Genistein  – Therapeutic use; Phytochemicals  – Physiological effect; Phytochemicals  – Therapeutic use; Prostate  –  Cancer  – Adjuvant treatment; Prostate  –  Cancer  – Cryptopathology; Prostate  –  Cancer  – Molecular aspects

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APA (6th Edition):

Oseni, S. O. (2015). Enhancement of the Chemopreventive and Chemotherapeutic Effects of Genistein and Beta-lapachone in Human Prostate Cancer Cells by Pyroelectrically Generated Very Low Dose Ionizing Radiation. (Masters Thesis). Florida Atlantic University. Retrieved from http://purl.flvc.org/fau/fd/FA00004530 ; (URL) http://purl.flvc.org/fau/fd/FA00004530

Chicago Manual of Style (16th Edition):

Oseni, Saheed Oluwasina. “Enhancement of the Chemopreventive and Chemotherapeutic Effects of Genistein and Beta-lapachone in Human Prostate Cancer Cells by Pyroelectrically Generated Very Low Dose Ionizing Radiation.” 2015. Masters Thesis, Florida Atlantic University. Accessed November 14, 2019. http://purl.flvc.org/fau/fd/FA00004530 ; (URL) http://purl.flvc.org/fau/fd/FA00004530.

MLA Handbook (7th Edition):

Oseni, Saheed Oluwasina. “Enhancement of the Chemopreventive and Chemotherapeutic Effects of Genistein and Beta-lapachone in Human Prostate Cancer Cells by Pyroelectrically Generated Very Low Dose Ionizing Radiation.” 2015. Web. 14 Nov 2019.

Vancouver:

Oseni SO. Enhancement of the Chemopreventive and Chemotherapeutic Effects of Genistein and Beta-lapachone in Human Prostate Cancer Cells by Pyroelectrically Generated Very Low Dose Ionizing Radiation. [Internet] [Masters thesis]. Florida Atlantic University; 2015. [cited 2019 Nov 14]. Available from: http://purl.flvc.org/fau/fd/FA00004530 ; (URL) http://purl.flvc.org/fau/fd/FA00004530.

Council of Science Editors:

Oseni SO. Enhancement of the Chemopreventive and Chemotherapeutic Effects of Genistein and Beta-lapachone in Human Prostate Cancer Cells by Pyroelectrically Generated Very Low Dose Ionizing Radiation. [Masters Thesis]. Florida Atlantic University; 2015. Available from: http://purl.flvc.org/fau/fd/FA00004530 ; (URL) http://purl.flvc.org/fau/fd/FA00004530

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