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You searched for subject:(Prorocentrum lima). Showing records 1 – 2 of 2 total matches.

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Robert Gordon University

1. Praptiwi, Radisti Ayu. Optimisation of high value metabolite production from benthic marine dinoflagellate Prorocentrum lima.

Degree: PhD, 2014, Robert Gordon University

Toxins produced by harmful algal blooms (HABs) are known to pose contamination risks to seafood products (e.g. fish and shellfish) consumed by human. In order to control contamination risks, monitoring regimes have to be performed rigorously. The effort to monitor the amount of toxins in consumable products has to rely on continuous supply to analytical standards. The current work presents the strategy in optimising the production of major diarrhetic shellfish poisoning (DSP) toxins, OA and DTX1, from Prorocentrum lima. The organism is also known to produce peridinin, a carotenoid pigment that has been found to have pharmaceutical potential. Results from this study showed that cultivation of P. lima CCAP 1136/11 was still, although not completely, reliant on supply of natural seawater. Characterisation of compounds produced by P. lima CCAP 1136/11 in batch culture identified three major bioactive compounds (OA, DTX1 and peridinin) and two minor OA-related compounds. Recovery of these major compounds was further optimised with two-stage extraction procedure. Several important considerations for the cultivation process include standardisation of inoculum age and initial cell density. These and several other growth parameters such as temperature, light and CO2 supplementation have been shown to affect the growth and production of DSP toxins and peridinin in the culture. One of the main highlights in this study revealed that providing culture with light and dark cycle at frequency of 0.5 hour benefit in the enhancement of OA, DTX1 and peridinin yield from P. lima CCAP 1136/11. As the last part of this study, a simple and scalable design of reactor has been proposed. Contrary to common observations for dinoflagellate culture, P. lima CCAP 1136/11 was found to be able to withstand increased sparging within the culture system, resulting in concomitant increased of growth and production of OA, DTX1 and peridinin. Future works have been suggested to focus on: (1) exploitation of different cultivation system, such as continuous or semicontinuous systems, and (2) exploration on genetic modification to enable commercial scale production of DSP toxins and peridinin.

Subjects/Keywords: 610; Dinoflagellate; Prorocentrum lima; Okadaic acid; Dinophysistoxin-1; Photobioreactor

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6th Edition):

Praptiwi, R. A. (2014). Optimisation of high value metabolite production from benthic marine dinoflagellate Prorocentrum lima. (Doctoral Dissertation). Robert Gordon University. Retrieved from http://hdl.handle.net/10059/1006

Chicago Manual of Style (16th Edition):

Praptiwi, Radisti Ayu. “Optimisation of high value metabolite production from benthic marine dinoflagellate Prorocentrum lima.” 2014. Doctoral Dissertation, Robert Gordon University. Accessed July 07, 2020. http://hdl.handle.net/10059/1006.

MLA Handbook (7th Edition):

Praptiwi, Radisti Ayu. “Optimisation of high value metabolite production from benthic marine dinoflagellate Prorocentrum lima.” 2014. Web. 07 Jul 2020.

Vancouver:

Praptiwi RA. Optimisation of high value metabolite production from benthic marine dinoflagellate Prorocentrum lima. [Internet] [Doctoral dissertation]. Robert Gordon University; 2014. [cited 2020 Jul 07]. Available from: http://hdl.handle.net/10059/1006.

Council of Science Editors:

Praptiwi RA. Optimisation of high value metabolite production from benthic marine dinoflagellate Prorocentrum lima. [Doctoral Dissertation]. Robert Gordon University; 2014. Available from: http://hdl.handle.net/10059/1006

2. Varkitzi, Ioanna. Οικολογία επιβλαβών μικροφυκών και επίδραση στο θαλάσσιο βενθικό οικοσύστημα.

Degree: 2010, National and Kapodistrian University of Athens; Εθνικό και Καποδιστριακό Πανεπιστήμιο Αθηνών (ΕΚΠΑ)

Harmful algae attract interest due to the increase of duration and intenseness of Harmful Algal Blooms (HABs), with serious impacts on coastal ecosystems, public health and economy. Many harmful algae are toxic microalgae that are consumed by pelagic and benthic organisms. The subject of this Doctoral Thesis was the study of ecology and toxicity of the dinoflagellates Dinophysis acuminata and Prorocentrum lima and the diatom Pseudo-nitzschia multiseries, as well as the transfer of their toxins to the bivalves Callista chione and Ruditapes decussatus. The studied microalgae species cause HABs in many coastal areas, including South Europe, e.g. Greece and Spain, while the studied bivalve species present wide distribution in these two counties and commercial interest. The aims of the Thesis were the study of: a) the natural populations and HABs of D. acuminata in relation to the availability of organic matter and other biotic/abiotic parameters in the Gulf of Thermaikos, b) the impact of nitrogen or phosphorus deficiency conditions on the growth and toxicity of P. lima, and c) the accumulation and impact of D. acuminata, P. lima και P. multiseries toxins on the bivalves C. chione from Thermaikos Gulf and R. decussatus from Ría de Vigo, Spain.

Τα επιβλαβή φύκη παρουσιάζουν ενδιαφέρον καθώς η ένταση και η διάρκεια των εξάρσεών τους αυξάνονται με σοβαρές επιπτώσεις στα παράκτια οικοσυστήματα, τη δημόσια υγεία και οικονομία. Πολλά επιβλαβή φύκη είναι τοξικά μικροφύκη και καταναλώνονται από πελαγικούς και βενθικούς οργανισμούς. Αντικείμενο της Διδακτορικής Διατριβής αποτέλεσε η μελέτη της οικολογίας και τοξικότητας των δινομαστιγωτών Dinophysis acuminata και Prorocentrum lima και του διατόμου Pseudo-nitzschia multiseries, καθώς και η μεταφορά των τοξινών τους στα δίθυρα μαλάκια Callista chione και Ruditapes decussatus. Αυτά τα είδη μικροφυκών προκαλούν επιβλαβείς εξάρσεις σε πολλές παράκτιες περιοχές συμπεριλαμβανομένης της Ν Ευρώπης, όπως η Ελλάδα και η Ισπανία, τα δε είδη διθύρων παρουσιάζουν εκεί ευρεία εξάπλωση και εμπορικό ενδιαφέρον. Στόχοι της Διατριβής ήταν: α) η μελέτη των φυσικών πληθυσμών και των εξάρσεων του είδους D. acuminata σε σχέση με τη διαθεσιμότητα της οργανικής ύλης και άλλων βιοτικών/αβιοτικών παραμέτρων στον Θερμαϊκό Κόλπο, β) η μελέτη της επίδρασης των συνθηκών έλλειψης αζώτου/φωσφόρου στην αύξηση του είδους P. lima και την παραγωγή τοξίνης στα κύτταρά του, γ) η μελέτη της συσσώρευσης και της επίδρασης των τοξινών που παράγουν τα μικροφύκη D. acuminata, P. lima, P. multiseries στα δίθυρα C. chione από τον Θερμαϊκό και R. decussatus από τον Κόλπο του Vigo της Ισπανίας.

Subjects/Keywords: Εξάρσεις επιβλαβών φυκών; Συσσώρευση τοξινών; Αποτοξίνωση; Harmful algal blooms; Toxins accumulation; Toxins depuration; Dinophysis acuminata; Prorocentrum lima; Pseudo-nitzschia multiseries; Callista chione; Ruditapes decussatus

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6th Edition):

Varkitzi, I. (2010). Οικολογία επιβλαβών μικροφυκών και επίδραση στο θαλάσσιο βενθικό οικοσύστημα. (Thesis). National and Kapodistrian University of Athens; Εθνικό και Καποδιστριακό Πανεπιστήμιο Αθηνών (ΕΚΠΑ). Retrieved from http://hdl.handle.net/10442/hedi/28363

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16th Edition):

Varkitzi, Ioanna. “Οικολογία επιβλαβών μικροφυκών και επίδραση στο θαλάσσιο βενθικό οικοσύστημα.” 2010. Thesis, National and Kapodistrian University of Athens; Εθνικό και Καποδιστριακό Πανεπιστήμιο Αθηνών (ΕΚΠΑ). Accessed July 07, 2020. http://hdl.handle.net/10442/hedi/28363.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7th Edition):

Varkitzi, Ioanna. “Οικολογία επιβλαβών μικροφυκών και επίδραση στο θαλάσσιο βενθικό οικοσύστημα.” 2010. Web. 07 Jul 2020.

Vancouver:

Varkitzi I. Οικολογία επιβλαβών μικροφυκών και επίδραση στο θαλάσσιο βενθικό οικοσύστημα. [Internet] [Thesis]. National and Kapodistrian University of Athens; Εθνικό και Καποδιστριακό Πανεπιστήμιο Αθηνών (ΕΚΠΑ); 2010. [cited 2020 Jul 07]. Available from: http://hdl.handle.net/10442/hedi/28363.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Varkitzi I. Οικολογία επιβλαβών μικροφυκών και επίδραση στο θαλάσσιο βενθικό οικοσύστημα. [Thesis]. National and Kapodistrian University of Athens; Εθνικό και Καποδιστριακό Πανεπιστήμιο Αθηνών (ΕΚΠΑ); 2010. Available from: http://hdl.handle.net/10442/hedi/28363

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

.