subject:(Polymersome)

Delft University of Technology

1. Sanders, T.J. (author). Suitability of Biodegradable Polymersomes as Radionuclide Carriers.

Degree: 2014, Delft University of Technology

URL: http://resolver.tudelft.nl/uuid:e085327f-947d-46f9-b1f3-a1b438e14f3f

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Suitability of biodegradable polymersomes functioning as radionuclide carriers is assessed. Biodegradable polymersomes employed in this research were composed of either diblock copolymers, poly(caprolactone)-poly(ethylene oxide)(PCL-PEO) or… (more)

Subjects/Keywords: polymersome; radionuclide; radiotherapy; biodegradable

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APA (6^th Edition):

Sanders, T. J. (. (2014). Suitability of Biodegradable Polymersomes as Radionuclide Carriers. (Masters Thesis). Delft University of Technology. Retrieved from http://resolver.tudelft.nl/uuid:e085327f-947d-46f9-b1f3-a1b438e14f3f

Chicago Manual of Style (16^th Edition):

Sanders, T J (author). “Suitability of Biodegradable Polymersomes as Radionuclide Carriers.” 2014. Masters Thesis, Delft University of Technology. Accessed January 23, 2021. http://resolver.tudelft.nl/uuid:e085327f-947d-46f9-b1f3-a1b438e14f3f.

MLA Handbook (7^th Edition):

Sanders, T J (author). “Suitability of Biodegradable Polymersomes as Radionuclide Carriers.” 2014. Web. 23 Jan 2021.

Vancouver:

Sanders TJ(. Suitability of Biodegradable Polymersomes as Radionuclide Carriers. [Internet] [Masters thesis]. Delft University of Technology; 2014. [cited 2021 Jan 23]. Available from: http://resolver.tudelft.nl/uuid:e085327f-947d-46f9-b1f3-a1b438e14f3f.

Council of Science Editors:

Sanders TJ(. Suitability of Biodegradable Polymersomes as Radionuclide Carriers. [Masters Thesis]. Delft University of Technology; 2014. Available from: http://resolver.tudelft.nl/uuid:e085327f-947d-46f9-b1f3-a1b438e14f3f

Delft University of Technology

2. Bogers, S.L.C. (author). Determining the state of radionuclides in polymersomes using perturbed angular correlation spectroscopy.

Degree: 2015, Delft University of Technology

URL: http://resolver.tudelft.nl/uuid:bc2d79e4-d287-4c9c-b9ad-6504d3f256db

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Polymersomes, polymeric nano-carriers composed of amphiphilic block copolymers, are promising candidates for transporting (radio)pharmaceuticals to tumour cells. While methods have been developed for trapping both… (more)

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Polymersomes, polymeric nano-carriers composed of amphiphilic block copolymers, are promising candidates for transporting (radio)pharmaceuticals to tumour cells. While methods have been developed for trapping both diagnostic and therapeutic amounts of radionuclides in these polymersomes, there is not much known about the loading process and the state of the radionuclide in the polymersomes. At this point perturbed angular correlation (PAC) spectroscopy becomes essential, as it is a useful method for gaining information about the chemical surroundings and physical structures of different gamma-emitting radioisotopes. PAC is a gamma ray spectroscopy technique which can be used for the investigation of hyperfine interactions. A hyperfine interaction is the interaction between a nucleus and its environment. Hyperfine interactions arise in the intermediate state of a decaying radionuclide. This decay is then perturbed with a time-dependent factor and can be measured from the angular correlation function. The angular correlation function is expressed as the number of coincidences per unit time as function of angle. The number of coincidences is measured using the PAC spectroscopy. The polymersomes are composed of poly(butadiene-b-ethylene oxide) block copolymers, and the labelling has been achieved by transportation of the radionuclide, complexed to a lipophilic ligand, through the hydrophobic bilayer into the aqueous cavity containing 0.5 M of KH2PO4. A sufficient amount of the radionuclides was encapsulated in the polymersomes (>90 % loading efficiency). Subsequent to the loading, the sample was immersed in liquid nitrogen, after it was counted on the PAC setup for 6 hours at 90 and 180 angles. To confirm a working PAC setup, measurements on directional correlation and a simple perturbed system are performed. Excellent agreement with literature has been observed for cobalt-60 directional correlation measurements (G22 of 0.1016 vs 0.1020 in [18]). The hafnium metal measurements, which only undergo a static interaction, are compared with literature [39]. The results give an indication of a reliable PAC setup. Final measurements are performed with indium-111. The results obtained for the loaded radionuclides in polymersomes indicate that differences could be distinguished. The results indicate that the radionuclides will move from the bilayer towards the core of the polymersome. There was no clear bonding observed between the chelator and the radionuclides. The used side of the polymersomes (100 nm, 800 nm) are expected not to influence the measurement.

Medical Physics, RIH, RID

BioMechanical Engineering

Mechanical, Maritime and Materials Engineering

Subjects/Keywords: perturbed angular correlation; polymersome

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Bogers, S. L. C. (. (2015). Determining the state of radionuclides in polymersomes using perturbed angular correlation spectroscopy. (Masters Thesis). Delft University of Technology. Retrieved from http://resolver.tudelft.nl/uuid:bc2d79e4-d287-4c9c-b9ad-6504d3f256db

Chicago Manual of Style (16^th Edition):

Bogers, S L C (author). “Determining the state of radionuclides in polymersomes using perturbed angular correlation spectroscopy.” 2015. Masters Thesis, Delft University of Technology. Accessed January 23, 2021. http://resolver.tudelft.nl/uuid:bc2d79e4-d287-4c9c-b9ad-6504d3f256db.

MLA Handbook (7^th Edition):

Bogers, S L C (author). “Determining the state of radionuclides in polymersomes using perturbed angular correlation spectroscopy.” 2015. Web. 23 Jan 2021.

Vancouver:

Bogers SLC(. Determining the state of radionuclides in polymersomes using perturbed angular correlation spectroscopy. [Internet] [Masters thesis]. Delft University of Technology; 2015. [cited 2021 Jan 23]. Available from: http://resolver.tudelft.nl/uuid:bc2d79e4-d287-4c9c-b9ad-6504d3f256db.

Council of Science Editors:

Bogers SLC(. Determining the state of radionuclides in polymersomes using perturbed angular correlation spectroscopy. [Masters Thesis]. Delft University of Technology; 2015. Available from: http://resolver.tudelft.nl/uuid:bc2d79e4-d287-4c9c-b9ad-6504d3f256db

University of New South Wales

3. Wong, Chin Ken. Self-Assembly of Complex Functional Polymersomes.

Degree: Chemistry, 2018, University of New South Wales

URL: http://handle.unsw.edu.au/1959.4/59664 ; https://unsworks.unsw.edu.au/fapi/datastream/unsworks:49266/SOURCE02?view=true

► Amphiphilic block copolymers are nanoscale building blocks that have the ability to self-assemble into a variety of morphologies in dilute solution. This Thesis focuses primarily… (more)

▼ Amphiphilic block copolymers are nanoscale building blocks that have the ability to self-assemble into a variety of morphologies in dilute solution. This Thesis focuses primarily on polymersomes, which are one of the many classes of self-assembled block copolymer nanostructures. Polymersomes are hollow membrane sacs that are not only able to encapsulate hydrophobic and/or hydrophilic molecules, but also possess exceptional chemical and physical stability, structural versatility, and surface modifiability. For the above reasons, polymersomes have in recent years emerged as a powerful tool for a wide range of applications in the fields of drug delivery and biomimicry. With that being said, the full potential of polymersomes has yet to be harnessed due to a lack of definitive methods for shape control.The overall objective of this Thesis was to develop approaches to direct the self-assembly of block copolymer building blocks into complex yet functional polymersomes with shapes that deviate from a traditional sphere. To this end, a series of novel block copolymers with carefully incorporated supramolecular interaction motifs were synthesized and shown to be useful building blocks for the generation of spherical and non-spherical polymersomes; specifically, ones that are ellipsoidal, tubular or polyhedral shaped. The underlying mechanisms that govern the formation and shape transformation of these non-spherical polymersomes were also studied in detail.Some of the spherical and non-spherical polymersomes prepared were subsequently used for studies in vitro to understand how variations in polymersome shape and size influence their interactions with cells. In the final section of this Thesis, a protein-polymer bioconjugate with thermoresponsive properties was synthesized and shown to reversibly self-assemble into polymersomes that synergistically exhibit properties of both protein and polymer components. The ability of these biohybrid polymersomes to selectively (co-)encapsulate drug and protein molecules within different compartments was further demonstrated to highlight their potential use as advanced drug delivery vehicles.The findings reported in this Thesis should open up an avenue of opportunities for those who utilize polymersomes for drug delivery applications or as artificial cell and organelle scaffolds, as well as researchers interested in the fundamental aspects of polymer self-assembly and supramolecular chemistry. Advisors/Committee Members: Thordarson, Pall, Chemistry, Faculty of Science, UNSW, Stenzel, Martina, Chemistry, Faculty of Science, UNSW.

Subjects/Keywords: Drug delivery; Polymersome; Self-assembly

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Wong, C. K. (2018). Self-Assembly of Complex Functional Polymersomes. (Doctoral Dissertation). University of New South Wales. Retrieved from http://handle.unsw.edu.au/1959.4/59664 ; https://unsworks.unsw.edu.au/fapi/datastream/unsworks:49266/SOURCE02?view=true

Chicago Manual of Style (16^th Edition):

Wong, Chin Ken. “Self-Assembly of Complex Functional Polymersomes.” 2018. Doctoral Dissertation, University of New South Wales. Accessed January 23, 2021. http://handle.unsw.edu.au/1959.4/59664 ; https://unsworks.unsw.edu.au/fapi/datastream/unsworks:49266/SOURCE02?view=true.

MLA Handbook (7^th Edition):

Wong, Chin Ken. “Self-Assembly of Complex Functional Polymersomes.” 2018. Web. 23 Jan 2021.

Vancouver:

Wong CK. Self-Assembly of Complex Functional Polymersomes. [Internet] [Doctoral dissertation]. University of New South Wales; 2018. [cited 2021 Jan 23]. Available from: http://handle.unsw.edu.au/1959.4/59664 ; https://unsworks.unsw.edu.au/fapi/datastream/unsworks:49266/SOURCE02?view=true.

Council of Science Editors:

Wong CK. Self-Assembly of Complex Functional Polymersomes. [Doctoral Dissertation]. University of New South Wales; 2018. Available from: http://handle.unsw.edu.au/1959.4/59664 ; https://unsworks.unsw.edu.au/fapi/datastream/unsworks:49266/SOURCE02?view=true

University of New South Wales

4. Mason, Alex. Polymersomes as synthetic cell scaffolds.

Degree: Chemistry, 2017, University of New South Wales

URL: http://handle.unsw.edu.au/1959.4/57315 ; https://unsworks.unsw.edu.au/fapi/datastream/unsworks:43279/SOURCE02?view=true

► Synthetic cell research is aimed at the de novo design of a system that displays all the hallmarks of life metabolism, reproduction, and compartmentalisation. Compartmentalisation,… (more)

▼ Synthetic cell research is aimed at the de novo design of a system that displays all the hallmarks of life metabolism, reproduction, and compartmentalisation. Compartmentalisation, or the separation of a cell or organelle from its outer environment with a lipid bilayer membrane, is key to life in that it enables complex, multi-step reactions to happen in an incredibly efficient manner. For decades, those wanting to mimic natural membranes have turned to liposomes, which are synthetic vesicles comprised of naturally occurring phospholipids. While well understood, liposomes are highly dynamic and thermodynamically unstable. Life is adept at controlling the self-assembly of lipid membranes into highly chemically and structurally asymmetric structures, but without metabolism and the input of energy it is impossible for us to achieve this currently.Polymer vesicles, or polymersomes, are comprised of larger, more inert amphiphilic block copolymers, which allow the generation of new, interesting structures that are thermodynamically stable and allow membrane-bound phenomena to be studied on longer timescales. Of particular interest is the interaction of naturally occurring enzymes with membranes, and the function of transmembrane proteins.This Thesis describes the advancement of the polymersome field for use in synthetic cell research. At the core of this work was the synthesis of a range of amphiphilic di- and triblock copolymers, designed to self-assemble into structures with biomimetic chemical asymmetry to facilitate the incorporation of membrane proteins. The self-assembly behaviour of these polymers was then investigated in a high throughput manner, and several protein bioconjugates were synthesised in order to control several key proteins of the electron transport chain with light. Finally, these naturally occurring proteins were incorporated into polymersomes, creating hybrid protein-polymer systems with structural and functional similarity to the natural mitochondria. Advisors/Committee Members: Thordarson, Pall, Chemistry, Faculty of Science, UNSW.

Subjects/Keywords: Block copolymers; Polymersome; Synthetic cell

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Mason, A. (2017). Polymersomes as synthetic cell scaffolds. (Doctoral Dissertation). University of New South Wales. Retrieved from http://handle.unsw.edu.au/1959.4/57315 ; https://unsworks.unsw.edu.au/fapi/datastream/unsworks:43279/SOURCE02?view=true

Chicago Manual of Style (16^th Edition):

Mason, Alex. “Polymersomes as synthetic cell scaffolds.” 2017. Doctoral Dissertation, University of New South Wales. Accessed January 23, 2021. http://handle.unsw.edu.au/1959.4/57315 ; https://unsworks.unsw.edu.au/fapi/datastream/unsworks:43279/SOURCE02?view=true.

MLA Handbook (7^th Edition):

Mason, Alex. “Polymersomes as synthetic cell scaffolds.” 2017. Web. 23 Jan 2021.

Vancouver:

Mason A. Polymersomes as synthetic cell scaffolds. [Internet] [Doctoral dissertation]. University of New South Wales; 2017. [cited 2021 Jan 23]. Available from: http://handle.unsw.edu.au/1959.4/57315 ; https://unsworks.unsw.edu.au/fapi/datastream/unsworks:43279/SOURCE02?view=true.

Council of Science Editors:

Mason A. Polymersomes as synthetic cell scaffolds. [Doctoral Dissertation]. University of New South Wales; 2017. Available from: http://handle.unsw.edu.au/1959.4/57315 ; https://unsworks.unsw.edu.au/fapi/datastream/unsworks:43279/SOURCE02?view=true

Johannes Gutenberg Universität Mainz

5. Müller, Waltraut. Hydrophobe und hydrophile Beladung polymerer Vesikel.

Degree: 2009, Johannes Gutenberg Universität Mainz

URL: http://ubm.opus.hbz-nrw.de/volltexte/2009/2128/

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Polymere Vesikel, gebildet durch Selbstorganisation des amphiphilen Blockcopolymers Polybutadien-b-Polyethylenoxid in Wasser, wurden in der vorliegenden Arbeit erfolgreich mit hydrophoben und hydrophilen Substraten beladen und detailliert… (more)

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Polymere Vesikel, gebildet durch Selbstorganisation des amphiphilen Blockcopolymers Polybutadien-b-Polyethylenoxid in Wasser, wurden in der vorliegenden Arbeit erfolgreich mit hydrophoben und hydrophilen Substraten beladen und detailliert charakterisiert. Über verschiedene Präparationsmethoden sind unilamellare PB130-PEO66-Vesikel unterschiedlicher Größen und Verteilungsbreiten zugänglich, die aber alle eine konstante hydrophobe Schalendicke von etwa 15nm aufweisen, wie aus TEM-Messungen hervorgeht. Die hydrophoben Farbstoffe Oil Red EGN, Oil Blue N, Nilrot sowie ein Perylen-Derivat wurden in diese hydrophobe Schale eingelagert. Durch Absorptions-, Emissions-, (cryo)TEM- und Fluoreszenzmikroskopie-Messungen konnte gezeigt werden, dass die selbstorganisierte Struktur durch die Einlagerung der hydrophoben Farbstoffe in die Schale nicht beeinflusst wird. Als zusätzliche hydrophobe Modell-Substrate wurden Halbleiter-Nanokristalle, sogenannte Quantum Dots (QDs, d=5.7nm), erfolgreich in die polymere Vesikelschale eingelagert und durch Fluoreszenz-Korrelations-Spektroskopie (FCS) in Kombination mit dynamischer Lichtstreuung (DLS) nachgewiesen. Die Position der QDs in der Mitte der polymeren Doppelmembran konnte durch cryogene TEM-Abbildungen aufgezeigt werden. Darüber hinaus wurde die hydrophile Beladung des Vesikelkerns mit dem wasserlöslichen Farbstoff Phloxin B erfolgreich realisiert.

Hydrophobic and hydrophilic loading of polymeric vesicles. The successful encapsulation of hydrophobic and hydrophilic substrates into poly(butadiene)-poly(ethylene oxide) vesicles in water is reported. Furthermore, the detailed characterization of these systems is presented. Different preparation methods were used to obtain unilamellar vesicles of PB130-PEO66 with different vesicle sizes and distributions. However, for all vesicles a common hydrophobic shell thickness of about 15nm was assembled as determined by TEM. The hydrophobic dyes Oil Red EGN, Oil Blue N, Nile Red and a Perylen derivative were embedded into this hydrophobic shell. Absorption-, emission-, (cryo)TEM- and fluorescencemicroscopy-measurements showed the self-assembled structure to remain unchanged when the hydrophobic substrates are incorporated within the vesicle shell. Highly fluorescent semiconductor nanocrystals, so called quantum dots (QDs, d=5.7nm), were selected as additional hydrophobic model substrates and successfully enclosed into the vesicle shell, as evidenced by fluorescence correlation spectroscopy (FCS) measurements in combination with dynamic light scattering (DLS). Cryogenic TEM imaging revealed the position of the QDs, centered inside the double layer of the vesicle shell. Furthermore, we successfully studied the hydrophilic loading into the vesicle core using the hydrophilic dye Phloxine B.

Subjects/Keywords: Vesikel, Polymersome, Beladung; Vesicle, Polymersome, Loading; Chemistry and allied sciences

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Müller, W. (2009). Hydrophobe und hydrophile Beladung polymerer Vesikel. (Doctoral Dissertation). Johannes Gutenberg Universität Mainz. Retrieved from http://ubm.opus.hbz-nrw.de/volltexte/2009/2128/

Chicago Manual of Style (16^th Edition):

Müller, Waltraut. “Hydrophobe und hydrophile Beladung polymerer Vesikel.” 2009. Doctoral Dissertation, Johannes Gutenberg Universität Mainz. Accessed January 23, 2021. http://ubm.opus.hbz-nrw.de/volltexte/2009/2128/.

MLA Handbook (7^th Edition):

Müller, Waltraut. “Hydrophobe und hydrophile Beladung polymerer Vesikel.” 2009. Web. 23 Jan 2021.

Vancouver:

Müller W. Hydrophobe und hydrophile Beladung polymerer Vesikel. [Internet] [Doctoral dissertation]. Johannes Gutenberg Universität Mainz; 2009. [cited 2021 Jan 23]. Available from: http://ubm.opus.hbz-nrw.de/volltexte/2009/2128/.

Council of Science Editors:

Müller W. Hydrophobe und hydrophile Beladung polymerer Vesikel. [Doctoral Dissertation]. Johannes Gutenberg Universität Mainz; 2009. Available from: http://ubm.opus.hbz-nrw.de/volltexte/2009/2128/

Université de Bordeaux I

6. Wan, Yali. Développement de nanovecteurs polymériques et lipidiques fonctionnalisés par des anticorps pour cibler des cellules cancéreuses : Development of antibody functionalized polymeric and lipidic nanoparticles for targeting cancer cells.

Degree: Docteur es, Biochimie, 2012, Université de Bordeaux I

URL: http://www.theses.fr/2012BOR14736

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Ce travail, qui fait partie d’un projet européen, « NANOTHER », est focalisé sur la fonctionnalisation de nanoparticules polymériques et lipidiques fonctionnalisées par des anticorps Herceptine® pour… (more)

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Ce travail, qui fait partie d’un projet européen, « NANOTHER », est focalisé sur la fonctionnalisation de nanoparticules polymériques et lipidiques fonctionnalisées par des anticorps Herceptine® pour cibler des cellules du cancer du sein HER2+. Deux stratégies de fonctionnalisation ont été étudiées : une a reposé sur l’utilisation de protéines de fusion, l’Anx5-ZZ, composée d’Annexine A5 et deux domaines Z homologues de la protéine A de Staphylococcus aureus qui peuvent se fixer des anticorps d’une manière orientée par leur fragment cristallisable ; l’autre a porté sur le couplage direct d’anticorps modifiés pour exposer des groupes sulfhydryles aux nanoparticules exposant des groupes maléimides.La première partie concerne le développement d’un agent de ciblage simplifié du complexe l’Anx5-ZZ-anticorps, à savoir l’Anx5-scFv (single-chain variable fragment). Puisque la cible n’avait pas été décidée au début de ce travail, deux scFvs ont été utilisé comme système modèle. L’expression de protéines de fusion a été essayée chez Escherichia Coli avec différentes constructions de protéines de fusion, différentes conditions d’expression et différentes souches bactériennes. Toutes les protéines sont soient agrégées soient non surexprimées.La deuxième partie consiste à fonctionnaliser les polymersomes par l’Herceptine® via l’Anx5-ZZ. D’abord, nous avons validé une méthode de modification de la surface de polymersome pour présenter des groupes maléimides. Ensuite, le couplage covalent de l’Anx5(SH)-ZZ aux polymersomes-maléimide a été réalisé et quantifié. Nous avons obtenu maximum 30 Anx5-ZZ par polymersome. Puis, la liaison d’affinité d’anticorps aux polymersomes-Anx5-ZZ a été caractérisée, réalisée et quantifiée. Pour 30 Anx5-ZZ par polymersome, nous avons 60 Herceptine® par polymersome. Cependant, l’efficacité de ciblage de ces systèmes est très faible.La troisième partie consiste à fonctionnaliser les liposomes par l’Herceptine® via couplage direct. Tout d’abord, la modification de l’Herceptine® pour présenter des groupes SH a été caractérisée et contrôlée. Ensuite, le couplage covalent d’Herceptine®-SH aux liposomes-maléimides a été réalisé et quantifié. L’étude de ciblage montre que les liposomes fonctionnalisés par une molécule d’Herceptine® sont capable de cibler les cellules HER2+.

This work, which is part of a European project "NANOTHER", focus on the functionalization of polymeric and lipidic nanoparticles by Herceptin® to target HER2+ cancer cells. Two functionalization strategies were studied: one was based on the use of a fusion protein, Anx5-ZZ, composed of Annexin A5 and two Z domains which are homologous with the protein A of Staphylococcus aureus that can bind antibodies by their crystallizable fragment in a oriented way; the other focused on the direct coupling of modified antibodies exposing sulfhydryl groups to nanoparticles exposing maleimid groups.The first part concerns the development of a targeting agent simplified from the Anx5-ZZ-antibody complex, namely Anx5-scFv (single-chain variable fragment).…

Advisors/Committee Members: Brisson, Alain (thesis director).

Subjects/Keywords: Polymersome; Liposome; Anx5-ZZ; Herceptine®; Fonctionnalisation; Cellules HER2+; Polymersome; Liposome; Anx5-ZZ; Herceptin®; Functionalization; HER2+ cells

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Wan, Y. (2012). Développement de nanovecteurs polymériques et lipidiques fonctionnalisés par des anticorps pour cibler des cellules cancéreuses : Development of antibody functionalized polymeric and lipidic nanoparticles for targeting cancer cells. (Doctoral Dissertation). Université de Bordeaux I. Retrieved from http://www.theses.fr/2012BOR14736

Chicago Manual of Style (16^th Edition):

Wan, Yali. “Développement de nanovecteurs polymériques et lipidiques fonctionnalisés par des anticorps pour cibler des cellules cancéreuses : Development of antibody functionalized polymeric and lipidic nanoparticles for targeting cancer cells.” 2012. Doctoral Dissertation, Université de Bordeaux I. Accessed January 23, 2021. http://www.theses.fr/2012BOR14736.

MLA Handbook (7^th Edition):

Wan, Yali. “Développement de nanovecteurs polymériques et lipidiques fonctionnalisés par des anticorps pour cibler des cellules cancéreuses : Development of antibody functionalized polymeric and lipidic nanoparticles for targeting cancer cells.” 2012. Web. 23 Jan 2021.

Vancouver:

Wan Y. Développement de nanovecteurs polymériques et lipidiques fonctionnalisés par des anticorps pour cibler des cellules cancéreuses : Development of antibody functionalized polymeric and lipidic nanoparticles for targeting cancer cells. [Internet] [Doctoral dissertation]. Université de Bordeaux I; 2012. [cited 2021 Jan 23]. Available from: http://www.theses.fr/2012BOR14736.

Council of Science Editors:

Wan Y. Développement de nanovecteurs polymériques et lipidiques fonctionnalisés par des anticorps pour cibler des cellules cancéreuses : Development of antibody functionalized polymeric and lipidic nanoparticles for targeting cancer cells. [Doctoral Dissertation]. Université de Bordeaux I; 2012. Available from: http://www.theses.fr/2012BOR14736

7. Babinot, Julien. Élaboration de copolymères amphiphiles à base de poly (3-hydroxyalcanoate)s : Design of poly (3-hydroxyalkanoates)-based amphiphilic copolymers.

Degree: Docteur es, Sciences des Matériaux, 2012, Université Paris-Est

URL: http://www.theses.fr/2012PEST1111

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Les poly (3-hydroxyalcanoates) (PHAs) sont des polyesters aliphatiques produits et accumulés par des bactéries en tant que réserve de carbone et d'énergie. Ils sont constitués… (more)

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Les poly (3-hydroxyalcanoates) (PHAs) sont des polyesters aliphatiques produits et accumulés par des bactéries en tant que réserve de carbone et d'énergie. Ils sont constitués d'unités β-hydroxyesters et possèdent des chaînes latérales de longueur variable, pouvant être fonctionnalisées. Ils possèdent des propriétés de biodégradabilité et de biocompatibilité; ceci leur confère de vastes possibilités d'utilisation dans le domaine biomédical, notamment pour la mise au point de systèmes de libération contrôlée de principes actifs. Dans cette optique, nous nous sommes intéressés à la synthèse de copolymères amphiphiles de différentes architectures à base de PHAs, ainsi qu'à l'étude de leurs propriétés d'auto-association en milieu aqueux. Une méthode simple et efficace permettant le greffage d'oligomères de poly (éthylène glycol) (PEG) a tout d'abord été mise au point grâce à l'utilisation de la chimie « click ». Une série de copolymères diblocs bien définis PHA-b-PEG a ainsi pu être synthétisée par cycloaddition de Huisgen catalysée par le cuivre (CuAAC). Les copolymères diblocs à base de PHAs à moyennes chaînes latérales (PHA-mcl) ont montré leur capacité à s'auto-associer en milieu aqueux et à former des micelles monodisperses présentant une concentration micellaire critique très faible. Par la suite des copolymères de type greffés PHOU-g-PEG ont été synthétisés par addition thiol-ène. Les analyses par cryo microscopie électronique à transmission (cryo-TEM) ont montré que dans ce cas les copolymères s'auto-associaient en structures vésiculaires, ou polymersomes. Enfin, la synthèse de copolymères amphiphiles greffés porteurs de chaînes perfluorées PHOU-g-(F;PEG) a permis l'obtention de structures auto-associées plus complexes. Le cryo-TEM a en effet révélé la formation de micelles multicompartimentées, c'est à dire possédant un coeur présentant une séparation de phase entre les domaines hydrophobes et les domaines fluorés. Des tests biologiques préliminaires ont montré la cytocompatibilité de ces micelles

Poly (3-hydroxyalkanoates) are natural aliphatic polyesters produced and accumulated by many bacteria as carbon and energy supply. They consist of β-hydroxy ester units, with pendant side chains of different lengths that can be functionalized. Thanks to their biodegradability and biocompatibility, they are promising polymers for biomedical applications, especially for controlled drug delivery systems. In this context, we aimed to synthesize PHA-based amphiphilic copolymers with different molecular architectures, and to study their self-assembly in water. First, a simple and straightforward method using click chemistry has been used to graft poly(ethylene glycol) (PEG) oligomers. A series of well-defined diblock copolymers PHA-b-PEG has thus been synthesized using copper-catalyzed azide-alkyne cycloaddition (CuAAC). Medium chain length PHA-based diblock copolymers have shown their ability to self-assemble into stable micelles having very low critical micelle concentrations. Afterwards, amphiphilic graft copolymers…

Advisors/Committee Members: Langlois, Valérie (thesis director).

Subjects/Keywords: Poly (3-hydroxyalcanoate); Copolymère amphiphile; Chimie; Micelle; Polymersome; Poly (3-hydroxyalkanoate); Amphiphilic copolymer; Click chemistry; Micelle; Polymersome

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Babinot, J. (2012). Élaboration de copolymères amphiphiles à base de poly (3-hydroxyalcanoate)s : Design of poly (3-hydroxyalkanoates)-based amphiphilic copolymers. (Doctoral Dissertation). Université Paris-Est. Retrieved from http://www.theses.fr/2012PEST1111

Chicago Manual of Style (16^th Edition):

Babinot, Julien. “Élaboration de copolymères amphiphiles à base de poly (3-hydroxyalcanoate)s : Design of poly (3-hydroxyalkanoates)-based amphiphilic copolymers.” 2012. Doctoral Dissertation, Université Paris-Est. Accessed January 23, 2021. http://www.theses.fr/2012PEST1111.

MLA Handbook (7^th Edition):

Babinot, Julien. “Élaboration de copolymères amphiphiles à base de poly (3-hydroxyalcanoate)s : Design of poly (3-hydroxyalkanoates)-based amphiphilic copolymers.” 2012. Web. 23 Jan 2021.

Vancouver:

Babinot J. Élaboration de copolymères amphiphiles à base de poly (3-hydroxyalcanoate)s : Design of poly (3-hydroxyalkanoates)-based amphiphilic copolymers. [Internet] [Doctoral dissertation]. Université Paris-Est; 2012. [cited 2021 Jan 23]. Available from: http://www.theses.fr/2012PEST1111.

Council of Science Editors:

Babinot J. Élaboration de copolymères amphiphiles à base de poly (3-hydroxyalcanoate)s : Design of poly (3-hydroxyalkanoates)-based amphiphilic copolymers. [Doctoral Dissertation]. Université Paris-Est; 2012. Available from: http://www.theses.fr/2012PEST1111

8. Wu, Xiaohan. Auto-assemblage et modélisation des copolymères à blocs amphiphiles polylactide/poly(éthylène glycol) de différentes structures de chaînes en solution aqueuse : Self-assembly and modeling of amphiphilic polylactide/poly (ethylene glycol) block copolymers with diverse chain structures in aqueous solution.

Degree: Docteur es, Biologie Santé, 2012, Université Montpellier I

URL: http://www.theses.fr/2012MON13507

►

Une série de copolymères à blocs amphiphiles polylactide/poly(éthylène glycol) (PLA/PEG) a été synthétisée par polymérisation par ouverture de cycles, chimie clic, ou couplage des homopolymères… (more)

▼

Une série de copolymères à blocs amphiphiles polylactide/poly(éthylène glycol) (PLA/PEG) a été synthétisée par polymérisation par ouverture de cycles, chimie clic, ou couplage des homopolymères PLA et PEG avec des extrémités de chaînes fonctionnels (OH, COOH ou NH2). Différentes configurations de PLA (PLLA et PDLA) ont été considérées pour élucider l'effet de stéréocomplexation sur les propriétés physico-chimiques des copolymères. Les copolymères présentent différentes structures de chaînes, y compris dibloc PEG-PLA, tribloc PLA-PEG-PLA du type ABA ou PEG-PLA-PEG asymétrique du type BAB-type. La masse molaire des copolymères varies de 2 000 à 20 000, et le rapport EO/LA de 1.0 to 7.0 environ. Différents agrégats formés par auto-assemblage tels que des nanotubes, polymersomes, filomicelles, micelles anisotropiques et micelles sphériques ont été obtenus par dissolution directe de copolymères, mélange de différents copolymères, ou mélange de copolymères avec surfactant dans une solution aqueuse. Des modèles théoriques ont été proposés pour simuler la formation des agrégats et expliquer l'évolution de leur taille et morphologie en fonction du rapport EO/LA, longueur de bloc, etc.

A series of amphiphilic polylactide/poly(ethylene glycol) (PLA/PEG) block copolymers was synthesized by ring-opening polymerization, click chemistry, or coupling PLA and PEG homopolymers with functional end groups (OH, COOH or NH2). Different configurations of PLA (PLLA and PDLA) were considered to elucidate the effect of stereocomplexation on the physico-chemical properties of copolymers. The obtained copolymers exhibit diverse chain structures, including PEG-PLA diblock, PLA-PEG-PLA ABA-type or asymmetric PEG-PLA-PEG BAB-type triblock structures. The molar mass of copolymers ranges from c.a. 2,000 to 20,000, and the EO/LA ratio from c.a. 1.0 to 7.0. Diverse self-assembled aggregates, including nanotubes, polymersomes, filomicelles, anisotropic micelles and spherical micelles, were obtained by dissolving the resulting copolymers, mixing different copolymers, or mixing copolymers with surfactant in aqueous solution. Theoretical models are proposed to simulate the formation of aggregates and to explain the evolution of their size and morphology as a function of EO/LA ratio, block length, etc.

Advisors/Committee Members: Li, Suming (thesis director).

Subjects/Keywords: Auto-assemblage; Polylactide; Poly(éthylène glycol); Modélisation; Micelle anisotropic; Polymersome; Self-assembly; Polylactide; Poly(ethylene glycol); Modeling; Anisotropic micelle; Polymersome; 570

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Wu, X. (2012). Auto-assemblage et modélisation des copolymères à blocs amphiphiles polylactide/poly(éthylène glycol) de différentes structures de chaînes en solution aqueuse : Self-assembly and modeling of amphiphilic polylactide/poly (ethylene glycol) block copolymers with diverse chain structures in aqueous solution. (Doctoral Dissertation). Université Montpellier I. Retrieved from http://www.theses.fr/2012MON13507

Chicago Manual of Style (16^th Edition):

Wu, Xiaohan. “Auto-assemblage et modélisation des copolymères à blocs amphiphiles polylactide/poly(éthylène glycol) de différentes structures de chaînes en solution aqueuse : Self-assembly and modeling of amphiphilic polylactide/poly (ethylene glycol) block copolymers with diverse chain structures in aqueous solution.” 2012. Doctoral Dissertation, Université Montpellier I. Accessed January 23, 2021. http://www.theses.fr/2012MON13507.

MLA Handbook (7^th Edition):

Wu, Xiaohan. “Auto-assemblage et modélisation des copolymères à blocs amphiphiles polylactide/poly(éthylène glycol) de différentes structures de chaînes en solution aqueuse : Self-assembly and modeling of amphiphilic polylactide/poly (ethylene glycol) block copolymers with diverse chain structures in aqueous solution.” 2012. Web. 23 Jan 2021.

Vancouver:

Wu X. Auto-assemblage et modélisation des copolymères à blocs amphiphiles polylactide/poly(éthylène glycol) de différentes structures de chaînes en solution aqueuse : Self-assembly and modeling of amphiphilic polylactide/poly (ethylene glycol) block copolymers with diverse chain structures in aqueous solution. [Internet] [Doctoral dissertation]. Université Montpellier I; 2012. [cited 2021 Jan 23]. Available from: http://www.theses.fr/2012MON13507.

Council of Science Editors:

Wu X. Auto-assemblage et modélisation des copolymères à blocs amphiphiles polylactide/poly(éthylène glycol) de différentes structures de chaînes en solution aqueuse : Self-assembly and modeling of amphiphilic polylactide/poly (ethylene glycol) block copolymers with diverse chain structures in aqueous solution. [Doctoral Dissertation]. Université Montpellier I; 2012. Available from: http://www.theses.fr/2012MON13507

9. Deng, Yangwei. Polymersomes stimulables à base de polypeptoids : Stimuli-responsive polymersomes based on polypeptoids.

Degree: Docteur es, Chimie Moléculaire, 2018, Paris Sciences et Lettres (ComUE)

URL: http://www.theses.fr/2018PSLEC021

►

L'auto-assemblage de copolymères à blocs amphiphiles a été un sujet d’actualité depuis de nombreuses années, et parmi eux la vésicule polymère, à savoir polymersome, a… (more)

▼

L'auto-assemblage de copolymères à blocs amphiphiles a été un sujet d’actualité depuis de nombreuses années, et parmi eux la vésicule polymère, à savoir polymersome, a suscité des intérêts particuliers grâce à sa structure membranaire similaire à celle de la cellule. Les polymersomes portent un grand potentiel d’application dans le domaine biomedial. Par conséquent, nous proposons dans cette thèse de développer des polymersomes biocompatibles et stimulables, qui sont formés de copolymères à blocs de poly(amino acide)s et de polymères cristaux liquides. Ces copolymères sont synthétisés par polymérisation par ouverture de cycle de NTA (ex, sarcosine N-thiocarboxyanhydride) développé récemment. Le projet de recherche comprend les aspects suivants: (1) synthétiser et caractériser de différents types de copolymères à blocs de de poly(amino acide)s et de polymères cristaux liquides; (2) Etudier leur auto-assemblage et analyser la relation entre le comportement d'auto-assemblage et la structure chimique, trouver la meilleure condition et comprendre le mécanisme de la formation de polymersome; (3) Préparer des polymersomes stimulables et étudier leur réponse à des stimuli (ex., réaction de réduction, changement de pH, etc.).

The self-assembly of amphiphilic block copolymers has been a hot issue for many years, and among them the self-assembled polymer vesicle, namely polymersome, has attracted much attention with its similar structure to cellular membrane and great potential in biomedial field. Hence we come up with an idea to combine the special liquid crystal (LC) layer structure with the novel ring-opening polymerization, in order to develop a new type of polymersomes with biocompatibility and stimuli-responsiveness. The research project includes the following aspects: (1) Synthesize different kinds of PSar-containing LC copolymers with clear structure characterization data; (2)Analyze the relationship between the self-assembly behavior and structure of the synthesized copolymers, obtain the condition for polymersome formation from the copolymers, and find out the formative mechanism; (3) Prepare stimuli-responsive and targeting polymersomes with additional chemical modification, and study their responsiveness in function of oxidation-reduction, pH, temperature, magnetic field,light etc.

Advisors/Committee Members: Li, Min Hui (thesis director).

Subjects/Keywords: Polymersome; Polypeptoid; Auto-Assemblage; Oxydation-Stimulable; Photo-Stimulable; Polymersome; Polypeptoid; Self-Assembly; Oxidation-Responsive; Oxidation-Responsive

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Deng, Y. (2018). Polymersomes stimulables à base de polypeptoids : Stimuli-responsive polymersomes based on polypeptoids. (Doctoral Dissertation). Paris Sciences et Lettres (ComUE). Retrieved from http://www.theses.fr/2018PSLEC021

Chicago Manual of Style (16^th Edition):

Deng, Yangwei. “Polymersomes stimulables à base de polypeptoids : Stimuli-responsive polymersomes based on polypeptoids.” 2018. Doctoral Dissertation, Paris Sciences et Lettres (ComUE). Accessed January 23, 2021. http://www.theses.fr/2018PSLEC021.

MLA Handbook (7^th Edition):

Deng, Yangwei. “Polymersomes stimulables à base de polypeptoids : Stimuli-responsive polymersomes based on polypeptoids.” 2018. Web. 23 Jan 2021.

Vancouver:

Deng Y. Polymersomes stimulables à base de polypeptoids : Stimuli-responsive polymersomes based on polypeptoids. [Internet] [Doctoral dissertation]. Paris Sciences et Lettres (ComUE); 2018. [cited 2021 Jan 23]. Available from: http://www.theses.fr/2018PSLEC021.

Council of Science Editors:

Deng Y. Polymersomes stimulables à base de polypeptoids : Stimuli-responsive polymersomes based on polypeptoids. [Doctoral Dissertation]. Paris Sciences et Lettres (ComUE); 2018. Available from: http://www.theses.fr/2018PSLEC021

University of Minnesota

10. So, Soonyong. Nanoemulsion-like Polymersomes for Nanoreactors.

Degree: PhD, Chemical Engineering, 2015, University of Minnesota

URL: http://hdl.handle.net/11299/175335

► Self-assembly of block copolymers in various selective solvents provides a means to control nanostructures. Among selective solvents, ionic liquids (ILs) are of great interest as… (more)

▼ Self-assembly of block copolymers in various selective solvents provides a means to control nanostructures. Among selective solvents, ionic liquids (ILs) are of great interest as reaction media, with the possibility of replacing organic solvents. However, the implementation ILs is limited by their high viscosity and cost. Phase transfer of IL-filled polymer vesicles (polymersomes) from the IL phase to water produces a very stable kind of "nanoemulsion"�. Nanoemulsion-like polymersomes have great potential as they confine a catalyst within the interiors, thus mitigating the mass transfer limitations of ILs while simultaneously providing a facile route to quantitative catalyst recovery The issues in the nanoreactor system and the mechanism of the phase transfer in the biphasic system are discussed. First, a new reversible reaction process with the thermo-responsive shuttling of the IL-filled polymersomes between the phases was designed. In nanoreactor applications, a narrowly distributed, small vesicle size is required. The size of polymersomes having rubbery and glassy membranes was controlled through mechanical and kinetic approaches. In the mechanical approach, the extrusion method was employed. For the kinetic approach, the amount of co-solvent and the hydrophilic fraction of amphiphilic block copolymer were varied and its effects on the size and dispersity were studied. Transport phenomena across the glassy and rubbery bilayer membranes was elucidated by NMR techniques to quantify the mobility inside and outside the polymersomes, plus the rate of exchange through the membrane. The dependence of the membrane thickness, glass transition temperature of the membranes and the partition coefficient of tracer molecules in the IL/water were also examined. We demonstrated a general boundary for the phase transfer of polymersomes in terms of a reduced tethering density for poly(ethylene oxide) (PEO), and analyzed the phenomena thermodynamically. The tethering density can be increased by increasing the block length of PEO and the size of the polymersomes, and the increased tethering density induces the phase transfer. Interfacial tension-related phase transfer led to develop a novel separation method in the biphasic system of the IL and water. By controlling the interfacial tension between the hydrophobic membrane and water, worm-like micelles and polymersomes were successfully separated.

Subjects/Keywords: Emulsion; Nanoreactor; Permeability; PFG-NMR; Phase transfer; Polymersome

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

So, S. (2015). Nanoemulsion-like Polymersomes for Nanoreactors. (Doctoral Dissertation). University of Minnesota. Retrieved from http://hdl.handle.net/11299/175335

Chicago Manual of Style (16^th Edition):

So, Soonyong. “Nanoemulsion-like Polymersomes for Nanoreactors.” 2015. Doctoral Dissertation, University of Minnesota. Accessed January 23, 2021. http://hdl.handle.net/11299/175335.

MLA Handbook (7^th Edition):

So, Soonyong. “Nanoemulsion-like Polymersomes for Nanoreactors.” 2015. Web. 23 Jan 2021.

Vancouver:

So S. Nanoemulsion-like Polymersomes for Nanoreactors. [Internet] [Doctoral dissertation]. University of Minnesota; 2015. [cited 2021 Jan 23]. Available from: http://hdl.handle.net/11299/175335.

Council of Science Editors:

So S. Nanoemulsion-like Polymersomes for Nanoreactors. [Doctoral Dissertation]. University of Minnesota; 2015. Available from: http://hdl.handle.net/11299/175335

University of New South Wales

11. Tjandra, Kristel. Cell-targeting peptides for selective drug delivery to tumour cells.

Degree: Chemistry, 2018, University of New South Wales

URL: http://handle.unsw.edu.au/1959.4/61937 ; https://unsworks.unsw.edu.au/fapi/datastream/unsworks:57812/SOURCE02?view=true

► Cancer stands out as a disease that could benefit immensely from targeted drug delivery. The presence of molecular biomarkers that differentiate cancer cells from their… (more)

▼ Cancer stands out as a disease that could benefit immensely from targeted drug delivery. The presence of molecular biomarkers that differentiate cancer cells from their surrounding cells is an often overlooked opportunity for developing a smart therapy with the ability to selectively target and terminate cells. Despite the increasing knowledge of cellular makeup, ligand identification remains a major setback in the development of effective and selective chemotherapies. The overall aim of this Thesis is to devise drug delivery systems suitable for harnessing the bioactivity of phage-derived cell-targeting peptides as a potential targeted treatment modality. This principal objective was explored by first producing a cell-targeting peptide for constructing a monomeric peptide-drug conjugate system, which was then used as a targeting ligand for a polymersome nanoparticle system.The successful synthesis of the cell-targeting peptide-drug conjugate using a phage-derived E1-3 peptide was followed by a series of in vitro studies performed to understand the viability of the phage-derived peptide as a drug carrier. These in vitro experiments revealed that the cell-targeting peptide derived from phage display screening could be conjugated to an anticancer drug without compromising its affinity to target cells. Furthermore, addition of this peptide resulted in an effective enhancement in drug selectivity towards its target medulloblastoma cells, DAOY. The promising outcomes from this system were extended to a nanoparticle system where the peptide moiety was incorporated onto the backbone of a diblock terpolymer. Self-assembly of this polymer produced polymersomes with a unique ellipsoidal shape. The advantage of this system was its tuneable properties, providing an excellent model for studying the effect of particle sizes, ligand density, and multivalency on nano-bio interactions. Subsequent biological evaluations of these polymersomes demonstrated the cell-targeting capability of these peptide-functionalised polymersomes towards its target. Preliminary testing on another sonic hedgehog medulloblastoma cell lines, UW228 also suggested the potential use of this system for targeting other cells in the same disease subgroup. The work described herein addresses some of the challenges and opportunities of cell-targeting peptides in the design of targeted drug delivery vehicles, which ultimately contribute to the rational design of targeted therapy. Advisors/Committee Members: Thordarson, Pall, Chemistry, Faculty of Science, UNSW.

Subjects/Keywords: Polymersome; Drug delivery; Peptide; Multivalency; Cancer; Targeting; Bioconjugation

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Tjandra, K. (2018). Cell-targeting peptides for selective drug delivery to tumour cells. (Doctoral Dissertation). University of New South Wales. Retrieved from http://handle.unsw.edu.au/1959.4/61937 ; https://unsworks.unsw.edu.au/fapi/datastream/unsworks:57812/SOURCE02?view=true

Chicago Manual of Style (16^th Edition):

Tjandra, Kristel. “Cell-targeting peptides for selective drug delivery to tumour cells.” 2018. Doctoral Dissertation, University of New South Wales. Accessed January 23, 2021. http://handle.unsw.edu.au/1959.4/61937 ; https://unsworks.unsw.edu.au/fapi/datastream/unsworks:57812/SOURCE02?view=true.

MLA Handbook (7^th Edition):

Tjandra, Kristel. “Cell-targeting peptides for selective drug delivery to tumour cells.” 2018. Web. 23 Jan 2021.

Vancouver:

Tjandra K. Cell-targeting peptides for selective drug delivery to tumour cells. [Internet] [Doctoral dissertation]. University of New South Wales; 2018. [cited 2021 Jan 23]. Available from: http://handle.unsw.edu.au/1959.4/61937 ; https://unsworks.unsw.edu.au/fapi/datastream/unsworks:57812/SOURCE02?view=true.

Council of Science Editors:

Tjandra K. Cell-targeting peptides for selective drug delivery to tumour cells. [Doctoral Dissertation]. University of New South Wales; 2018. Available from: http://handle.unsw.edu.au/1959.4/61937 ; https://unsworks.unsw.edu.au/fapi/datastream/unsworks:57812/SOURCE02?view=true

University of Pennsylvania

12. Sood, Nimil. Multi-Functional Polymer Vesicles: Applications in Chemotherapy and Photodynamic Therapy.

Degree: 2014, University of Pennsylvania

URL: https://repository.upenn.edu/edissertations/1450

► The field of drug delivery is rapidly expanding to bridge the gap between novel drugs that are created and their effective entry into diseased tissue.… (more)

▼ The field of drug delivery is rapidly expanding to bridge the gap between novel drugs that are created and their effective entry into diseased tissue. In one growing area of research, synthetic polymers are being utilized to meet these needs. The precise control over their chemistry allows polymers to be tuned to the drug delivery application and make them attractive candidates for research. The focus of this dissertation is to engineer responsive polymersomes for drug delivery and understand their ability to reduce drug toxicity, increase absorption in diseased cells and tissue, and control the release of drug in vitro and in vivo. Gemcitabine, a nucleoside analog, was encapsulated in the aqueous core of nano-polymersomes composed of the biodegradable and biocompatible polymer PEO-PCL, and the in vitro toxicity of this novel drug delivery construct was tested against Panc-1 cells. The polymersome formulation performed at par with the free drug with one-log cell killing at 1 Ã¬M of gemcitabine. The polymersome was also able to control the release of gemcitabine, and this release was modulated by the degradation kinetics of the ester linkages in the membrane. Photodynamic therapy was performed against OVCAR-5 (ovarian cancer) cells. A hydrophobic photosensitizer, benzoporphrin derivative monoacid A (BPD-MA) was encapsulated the in the membrane of polymersomes composed of PEO14-PBD22 (OB14.5) polymer, and its phototoxicity was compared to an existing photosensitizer formulation called verteporfin that is currently used in the clinic for age-related macular degeneration (AMD). The polymersome formulation outperformed verteporfin both at the in vitro and in vivo level. Additionally, we investigated the photorupture of giant polymersomes encapsulating a near IR fluorophore (porphyrin dimer, PZn2) in the hydrophobic membrane and dextran in the aqueous lumen. Polymersomes synthesized from softer polymers released more of a reporter dye than stiffer polymersomes when illuminated with 690 nm of light. Finally, we investigated the fractionation of giant polymersomes in a deterministic lateral displacement device and developed a hydrodynamic model to predict this fractionation based on the attractive and repulsive forces experienced by the polymersome.

Subjects/Keywords: chemotherapy; nanoparticle; photodynamic therapy; polymersome; Biomedical; Chemical Engineering

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Sood, N. (2014). Multi-Functional Polymer Vesicles: Applications in Chemotherapy and Photodynamic Therapy. (Thesis). University of Pennsylvania. Retrieved from https://repository.upenn.edu/edissertations/1450

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Sood, Nimil. “Multi-Functional Polymer Vesicles: Applications in Chemotherapy and Photodynamic Therapy.” 2014. Thesis, University of Pennsylvania. Accessed January 23, 2021. https://repository.upenn.edu/edissertations/1450.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Sood, Nimil. “Multi-Functional Polymer Vesicles: Applications in Chemotherapy and Photodynamic Therapy.” 2014. Web. 23 Jan 2021.

Vancouver:

Sood N. Multi-Functional Polymer Vesicles: Applications in Chemotherapy and Photodynamic Therapy. [Internet] [Thesis]. University of Pennsylvania; 2014. [cited 2021 Jan 23]. Available from: https://repository.upenn.edu/edissertations/1450.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Sood N. Multi-Functional Polymer Vesicles: Applications in Chemotherapy and Photodynamic Therapy. [Thesis]. University of Pennsylvania; 2014. Available from: https://repository.upenn.edu/edissertations/1450

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Alberta

13. Nish, Gordon L. Optimization of the reaction conditions of two enzymes for use in a carbon sequestration process, and investigation into immobilization via encapsulation within polymersomes.

Degree: MS, Department of Chemical and Materials Engineering, 2016, University of Alberta

URL: https://era.library.ualberta.ca/files/cv405s966t

► Carbon dioxide emissions from human activities contribute to an increase of greenhouse gases in the atmosphere. In nature, this gas is sequestered through the use… (more)

▼ Carbon dioxide emissions from human activities contribute to an increase of greenhouse gases in the atmosphere. In nature, this gas is sequestered through the use of enzymes found in the Calvin-Benson-Bassham cycle, with useful molecules such as sugars being synthesized as products. A biomimetic approach to capturing carbon dioxide and using it to synthesize useful chemicals, by way of these enzymes in a bioprocess, has been proposed. To achieve this, active enzymes must be harvested and their kinetic properties need to be characterized. Optimal process conditions must be established, with an appropriate enzyme immobilization technique being applied to ameliorate the overall bioprocess. In this work, the phosphoribulokinase enzyme was produced in the bacterial cell platform Escherichia coli using molecular cloning techniques. The kinetic conditions affecting the rates of the enzymes ribose 5-phosphate isomerase A from E. coli, and phosphoribulokinase from Synechococcus elongatus, were locally optimized through surface response methodology and mathematical modeling. These models predicted the optimal pH levels, temperature, substrate concentration and other factors used in the assays. The rate of ribose 5-phosphate isomerase product formation under optimized conditions showed an increase of about 37 % over the measured rate under initial conditions, while that of phosphoribulokinase activity increased around 21 %. Enzyme characterization revealed Km constants for the sugar substrates to be 0.12 mM for phosphoribulokinase and 9.4 mM for ribose 5-phosphate isomerase, with half-lives of 177 minutes and 89 hours, respectively, at room temperature. Furthermore, immobilization via encapsulation within polymersomes was investigated by using a digestive assay and fluorescence correlation spectroscopy. The encapsulation efficiency of the enzyme Rpi was found to be about 23 %. This represents a final enzyme concentration of 1.2 µM having been encapsulated. The enzyme phosphoribulokinase was successfully purified from a bacterial platform. This enzyme and ribose 5-phosphate isomerase were characterized using reaction kinetics, resulting in calculated half-lives and Michaelis constants. Locally optimized reaction conditions were found through experimental modeling, resulting in apparent increases in the reaction rate of both of the enzymes. The enzyme ribose 5-phosphate isomerase was successfully encapsulated within polymersomes.

Subjects/Keywords: polymersome; phosphoribulokinase; optimization; ribose 5-phosphate isomerase; encapsulation; immobilization; enzyme; carbon capture

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APA (6^th Edition):

Nish, G. L. (2016). Optimization of the reaction conditions of two enzymes for use in a carbon sequestration process, and investigation into immobilization via encapsulation within polymersomes. (Masters Thesis). University of Alberta. Retrieved from https://era.library.ualberta.ca/files/cv405s966t

Chicago Manual of Style (16^th Edition):

Nish, Gordon L. “Optimization of the reaction conditions of two enzymes for use in a carbon sequestration process, and investigation into immobilization via encapsulation within polymersomes.” 2016. Masters Thesis, University of Alberta. Accessed January 23, 2021. https://era.library.ualberta.ca/files/cv405s966t.

MLA Handbook (7^th Edition):

Nish, Gordon L. “Optimization of the reaction conditions of two enzymes for use in a carbon sequestration process, and investigation into immobilization via encapsulation within polymersomes.” 2016. Web. 23 Jan 2021.

Vancouver:

Nish GL. Optimization of the reaction conditions of two enzymes for use in a carbon sequestration process, and investigation into immobilization via encapsulation within polymersomes. [Internet] [Masters thesis]. University of Alberta; 2016. [cited 2021 Jan 23]. Available from: https://era.library.ualberta.ca/files/cv405s966t.

Council of Science Editors:

Nish GL. Optimization of the reaction conditions of two enzymes for use in a carbon sequestration process, and investigation into immobilization via encapsulation within polymersomes. [Masters Thesis]. University of Alberta; 2016. Available from: https://era.library.ualberta.ca/files/cv405s966t

University of Western Ontario

14. McIntosh, J Trevor. Stimuli-Responsive Polymersomes and Surface Functionalized Dendrimersomes: Platforms for Biomedical Applications.

Degree: 2015, University of Western Ontario

URL: https://ir.lib.uwo.ca/etd/3202

► Amphiphilic block copolymers (BCPs) and dendrimers are known to self-assemble in aqueous solution to form a number of aggregate morphologies. These different architectures are largely… (more)

Subjects/Keywords: Janus dendrimer; Dendrimersome; Surface functionalization; Polymersome; Photoresponsive; Self-assembly; Materials Chemistry; Organic Chemistry

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

McIntosh, J. T. (2015). Stimuli-Responsive Polymersomes and Surface Functionalized Dendrimersomes: Platforms for Biomedical Applications. (Thesis). University of Western Ontario. Retrieved from https://ir.lib.uwo.ca/etd/3202

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

McIntosh, J Trevor. “Stimuli-Responsive Polymersomes and Surface Functionalized Dendrimersomes: Platforms for Biomedical Applications.” 2015. Thesis, University of Western Ontario. Accessed January 23, 2021. https://ir.lib.uwo.ca/etd/3202.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

McIntosh, J Trevor. “Stimuli-Responsive Polymersomes and Surface Functionalized Dendrimersomes: Platforms for Biomedical Applications.” 2015. Web. 23 Jan 2021.

Vancouver:

McIntosh JT. Stimuli-Responsive Polymersomes and Surface Functionalized Dendrimersomes: Platforms for Biomedical Applications. [Internet] [Thesis]. University of Western Ontario; 2015. [cited 2021 Jan 23]. Available from: https://ir.lib.uwo.ca/etd/3202.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

McIntosh JT. Stimuli-Responsive Polymersomes and Surface Functionalized Dendrimersomes: Platforms for Biomedical Applications. [Thesis]. University of Western Ontario; 2015. Available from: https://ir.lib.uwo.ca/etd/3202

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of New South Wales

15. Beh, Chau Chun. Formation of Nano-carrier systems by dense gas processing.

Degree: Chemical Sciences & Engineering, 2013, University of New South Wales

URL: http://handle.unsw.edu.au/1959.4/52758 ; https://unsworks.unsw.edu.au/fapi/datastream/unsworks:11431/SOURCE01?view=true

► Nano-carriers such as liposomes, polymersomes and micelles find applications in the delivery of a wide range of compounds including targeted delivery of pharmaceuticals. In pharmaceutical… (more)

▼ Nano-carriers such as liposomes, polymersomes and micelles find applications in the delivery of a wide range of compounds including targeted delivery of pharmaceuticals. In pharmaceutical applications, liposomes are employed to increase the bioavailability, reduce toxicity and avoid undesirable interactions of active pharmaceutical ingredients.A number of conventional methods are used to produce nano-carriers. However, most of the conventional techniques require large amounts of organic solvents during the production and, hence, further purification steps are required. Additional purification is costly and may impose harsh working conditions on the nano-carriers that could damage the nano-carriers. Therefore, dense gas techniques have recently received attention for the production of nano-carriers. Dense gas techniques reduce, or even eliminate, the amount of organic solvents required by conventional methods, which make them ideal manufacturing techniques for nano-carriers for pharmaceutical application.In this work, a novel dense gas technique Depressurization of an Expanded Solution into Aqueous Media (DESAM) was used to produce liposomes, polymersomes, and micelles. The DESAM process is a fast single-step production method that is conducted at moderate pressures and temperatures. The liposomes, polymersomes and micelles generated by DESAM had average hydrodynamic diameters of 250 nm, 415 nm, and 100 nm respectively. The polymersomes and micelles produced had undetectable residual solvent by Gas Chromatography (GC). However, there was 2.2% v/v of ethanol detected in liposomes produced by the DESAM process.Although DESAM was used successfully to produce nano-carriers with minimal residual solvent, it is a batch process with limited scope for process optimisation. Hence, a novel semi-continuous dense gas technique was introduced to overcome the limitations of DESAM. In the semi-continuous process, removal of organic solvent is viable within the nano-carrier formation step. The nano-carriers produced using the semi-continuous process had undetectable residual solvent by GC. In addition, the new process provides more control over the experimental parameters than DESAM.Drug encapsulation for both liposomes and polymersomes was also studied by using the model drug isoniazid (anti-tuberculosis drug) in both DESAM and the semi-continuous processes. Both the processes capability to produce a range of drug-loaded nano-carriers has been demonstrated. Advisors/Committee Members: Foster, Neil, Chemical Sciences & Engineering, Faculty of Engineering, UNSW, Mammucari, Raffaella, Chemical Sciences & Engineering, Faculty of Engineering, UNSW.

Subjects/Keywords: Polymersome; Nano-carrier; Liposome; Micelle; Dense gas processing; Supercritical fluid; Drug delivery

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APA (6^th Edition):

Beh, C. C. (2013). Formation of Nano-carrier systems by dense gas processing. (Doctoral Dissertation). University of New South Wales. Retrieved from http://handle.unsw.edu.au/1959.4/52758 ; https://unsworks.unsw.edu.au/fapi/datastream/unsworks:11431/SOURCE01?view=true

Chicago Manual of Style (16^th Edition):

Beh, Chau Chun. “Formation of Nano-carrier systems by dense gas processing.” 2013. Doctoral Dissertation, University of New South Wales. Accessed January 23, 2021. http://handle.unsw.edu.au/1959.4/52758 ; https://unsworks.unsw.edu.au/fapi/datastream/unsworks:11431/SOURCE01?view=true.

MLA Handbook (7^th Edition):

Beh, Chau Chun. “Formation of Nano-carrier systems by dense gas processing.” 2013. Web. 23 Jan 2021.

Vancouver:

Beh CC. Formation of Nano-carrier systems by dense gas processing. [Internet] [Doctoral dissertation]. University of New South Wales; 2013. [cited 2021 Jan 23]. Available from: http://handle.unsw.edu.au/1959.4/52758 ; https://unsworks.unsw.edu.au/fapi/datastream/unsworks:11431/SOURCE01?view=true.

Council of Science Editors:

Beh CC. Formation of Nano-carrier systems by dense gas processing. [Doctoral Dissertation]. University of New South Wales; 2013. Available from: http://handle.unsw.edu.au/1959.4/52758 ; https://unsworks.unsw.edu.au/fapi/datastream/unsworks:11431/SOURCE01?view=true

Johannes Gutenberg Universität Mainz

16. Koch, Amelie Helena Ruth. Interactions of gold nanoparticles with polymeric membranes.

Degree: 2014, Johannes Gutenberg Universität Mainz

URL: http://ubm.opus.hbz-nrw.de/volltexte/2015/3976/

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This thesis focuses on the interactions of nanoparticles with artificial membranes. The synthesis of the block copolymer poly(dimethylsiloxane)-block-poly(2-methyloxazoline) (PDMS-b-PMOXA) is described, as well as the… (more)

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This thesis focuses on the interactions of nanoparticles with artificial membranes. The synthesis of the block copolymer poly(dimethylsiloxane)-block-poly(2-methyloxazoline) (PDMS-b-PMOXA) is described, as well as the formation of polymersomes in water. These polymersomes act as minimal cell models, consisting of an artificial bilayer membrane only, allowing the study of the interactions between nanoparticles and polymeric membranes. Both spherical and rod-shaped gold nanoparticles (AuNPs) were used in this study and they were characterized using light scattering (PCS), transmission electron microscopy (TEM), UV/Vis spectroscopy, and polarization anisotropy measurements. The polymer grafting on the spherical cores is asymmetric (shell asphericity) but is parallel to the inherent, due to polycrystallinity, core anisotropy, resulting in a characteristic scattering of the AuNPs in PCS.rnInteractions of polymersomes and AuNPs were investigated by PCS, cryo-TEM and UV/Vis. Three possible scenarios upon mixing of polymersomes and AuNPs can be distinguished by using only PCS: (i) no interactions between particles and vesicles, (ii) attachment of the particles to the outer side of the vesicles (decoration), and (iii) uptake of particles into the vesicles. It is shown that all three scenarios are possible, solely depending on the particle’s surface functionalization. In addition, it was revealed that the AuNPs need to be attached to the inner side of the membrane instead of diffusing freely within the vesicle. The present experimental findings essentially help with the understanding of the interactions of nanoparticles with membranes and show that the process of endocytosis can be attributed to physical processes only. rn

Diese Arbeit beschäftigt sich mit den Wechselwirkungen von Nanopartikel mit künstlichen Membranen. Die Synthese des Blockcopolymers Polydimethylsiloxan-block-Polymethyloxazoline (PDMS-b-PMOXA) wird beschrieben, sowie die anschließende Bildung von Polymersomen in Wasser. Diese Polymersome dienen dann als minimale Zellmodelle, bestehend aus einer künstlichen Membran, mit deren Hilfe die Aufnahme von Nanopartikel in Zellen untersucht wird. Als Modell-Nanopartikel dienen kugelförmige und stäbchenförmige Gold-Nanopartikel (AuNPs), die mittels Lichtstreuung (PCS), Transmissionselektronenmikroskopie (TEM), UV/Vis-Spektroskopie und Polarisations¬anisotropie-Messungen charakterisiert werden. Die Ausrichtung der aufgepfropften Polymerbürsten auf den sonst sphärischen Partikeln ist asymmetrisch und parallel zur Ausrichtung der internen Kristallinität der AuNP, wodurch sich ein charakteristisches Streuverhalten der Nanopartikel in PCS ergibt. rnDie Wechselwirkungen zwischen Polymersomen und AuNPs werden mittels PCS, cryo-TEM und UV/Vis-Spektroskopie untersucht. Drei mögliche Wechselwirkungs-Szenarien können allein mittels Charakterisierung durch PCS unterschieden werden: (i) keine Wechselwirkungen, (ii) Anhaftung der Partikel an die äußere Seite der Vesikel (Dekoration) und (iii) Aufnahme der Partikel in das Innere…

Subjects/Keywords: Polymersome; Gold Nanopartikel; Lichtstreuung; Endocytose; künstliche Zelle; polymersomes; gold nanoparticles; light scattering; endocytosis; artificial cell; Chemistry and allied sciences

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Koch, A. H. R. (2014). Interactions of gold nanoparticles with polymeric membranes. (Doctoral Dissertation). Johannes Gutenberg Universität Mainz. Retrieved from http://ubm.opus.hbz-nrw.de/volltexte/2015/3976/

Chicago Manual of Style (16^th Edition):

Koch, Amelie Helena Ruth. “Interactions of gold nanoparticles with polymeric membranes.” 2014. Doctoral Dissertation, Johannes Gutenberg Universität Mainz. Accessed January 23, 2021. http://ubm.opus.hbz-nrw.de/volltexte/2015/3976/.

MLA Handbook (7^th Edition):

Koch, Amelie Helena Ruth. “Interactions of gold nanoparticles with polymeric membranes.” 2014. Web. 23 Jan 2021.

Vancouver:

Koch AHR. Interactions of gold nanoparticles with polymeric membranes. [Internet] [Doctoral dissertation]. Johannes Gutenberg Universität Mainz; 2014. [cited 2021 Jan 23]. Available from: http://ubm.opus.hbz-nrw.de/volltexte/2015/3976/.

Council of Science Editors:

Koch AHR. Interactions of gold nanoparticles with polymeric membranes. [Doctoral Dissertation]. Johannes Gutenberg Universität Mainz; 2014. Available from: http://ubm.opus.hbz-nrw.de/volltexte/2015/3976/

University of Western Ontario

17. Nazemi, Ali. Development of Biodegradable and Stimuli-Responsive Macromolescules and Their Assemblies.

Degree: 2013, University of Western Ontario

URL: https://ir.lib.uwo.ca/etd/1715

► Polymersomes are potentially multifunctional soft materials constructed by the self-assembly of amphiphilic block copolymers in aqueous medium. While much research has focused on controlling the… (more)

▼ Polymersomes are potentially multifunctional soft materials constructed by the self-assembly of amphiphilic block copolymers in aqueous medium. While much research has focused on controlling the assembly and encapsulation properties of polymersomes, their surface functionalization has been relatively unexplored. This is important because it plays a critical role in determining their properties such as toxicity and biodistribution behavior. The work described in this thesis involves the development of a biocompatible and biodegradable polymersome systems based on poly(ethylene oxide)-b-polycaprolactone (PEO-PCL) block copolymers with azide surface groups as a novel scaffold for various biomedical applications. The surface functionalization of these polymersomes with polyester dendrons bearing alkyne focal points with different peripheral groups, such as amines and guanidines, as well as a small molecule rhodamine dye is accomplished and their conjugation yields are compared to each other. Moreover, dendritic and non-dendritic polymersome-based MRI contrast agents, with the highest currently reported longitudinal relaxivity for a polymersome system, are developed by decorating PEO-PCL polymerosomes' surfaces with both non-dendritic and dendritic Gd(III)-based contrast agents. In addition, PEO-PCL polymersomes were employed to develop a multifunctional system with the potential to interfere with the viral infection process at two levels. In addition to their use as materials for functionalizing the surfaces of nanomaterials, dendrimers and their assemblies have been widely used as drug delivery vehicles. In order to enable a new level of control over drug release, backbone photodegradable dendrimers and dendrons are synthesized by incorporation of a monomer unit based on o-nitrobenzyl esters and 2,2-bis(hydroxymethyl)propionic acid. It is shown that these dendrimers undergo effective photolysis to release only small molecules upon irradiation with UV light. Finally, these dendrons are incorporated into amphiphilic Janus dendrimer structures and their self-assembly to dendrimersomes followed by their photodegradation are discussed.

Subjects/Keywords: Polymersome; biodegradable; dendrimer; dendron; MRI contrast agent; influenza virus; photodegradable; dendrimersome; Materials Chemistry; Organic Chemistry; Polymer Chemistry

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APA (6^th Edition):

Nazemi, A. (2013). Development of Biodegradable and Stimuli-Responsive Macromolescules and Their Assemblies. (Thesis). University of Western Ontario. Retrieved from https://ir.lib.uwo.ca/etd/1715

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Nazemi, Ali. “Development of Biodegradable and Stimuli-Responsive Macromolescules and Their Assemblies.” 2013. Thesis, University of Western Ontario. Accessed January 23, 2021. https://ir.lib.uwo.ca/etd/1715.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Nazemi, Ali. “Development of Biodegradable and Stimuli-Responsive Macromolescules and Their Assemblies.” 2013. Web. 23 Jan 2021.

Vancouver:

Nazemi A. Development of Biodegradable and Stimuli-Responsive Macromolescules and Their Assemblies. [Internet] [Thesis]. University of Western Ontario; 2013. [cited 2021 Jan 23]. Available from: https://ir.lib.uwo.ca/etd/1715.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Nazemi A. Development of Biodegradable and Stimuli-Responsive Macromolescules and Their Assemblies. [Thesis]. University of Western Ontario; 2013. Available from: https://ir.lib.uwo.ca/etd/1715

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Illinois – Urbana-Champaign

18. Casey, Catherine M. Design and development of stimuli-responsive materials: pH sensitive polymersomes and poly(olefin sulfone)s.

Degree: PhD, Chemistry, 2016, University of Illinois – Urbana-Champaign

URL: http://hdl.handle.net/2142/95548

► Stimuli-responsive materials are materials exhibit a response when exposed to specific external triggers. These materials are powerful tools for material development in areas such as… (more)

▼ Stimuli-responsive materials are materials exhibit a response when exposed to specific external triggers. These materials are powerful tools for material development in areas such as encapsulation, photoresists, sensors, self-healing materials, drug delivery, and transient electronic devices. A variety of chemical triggers can be employed to stimulate materials, for this work the focus has been on pH-responsive materials. Materials which can be triggered with pH have a variety of applications in biological and industrial fields. This thesis is split into two main parts: 1) the development of acid-triggerable covalently-crosslinked polymersomes and 2) the design and synthesis of base-, heat-, and fluoride-sensitive poly(olefin sulfone)s. Polymersomes are a useful approach for encapsulation but are susceptible to environmental stressors and leakage. By tuning the nanoscale architecture of the polymersomes with reversible chemical modifications, their stability can be improved while still allowing triggered release capabilities that permanently cross-linked polymersomes lack. Using dynamic covalent imine chemistry, terminally functionalized polymers were reversibly connected within polymersome membranes in the presence of reactive linkers. The connection of these polymer was investigated using two polymersome systems, poly(styrene-b-acrylic acid) in Chapter 2 and poly(styrene-b-ethylene oxide) in Chapter 3. Poly(olefin sulfone)s are a class of polymers known to degrade in the presence of base, as well as through thermolysis and radiolysis. In order to develop novel materials for applications in encapsulation and transient electronic devices, molecular design criteria needed to achieve rapid, base degradation of poly(olefin sulfone)s at room temperature were investigated,. Poly(vinyl ester sulfone)s and poly(vinyl butyl carbonate sulfone)s were synthesized and shown to degrade more rapidly than aliphatic poly(olefin sulfone)s (Chapter 4 and 5). Additional work has focus on the design of fluoride sensitive poly(olefin sulfone)s and the modulation of the thermal degradation of poly(tert-butyl carbonate sulfone) and poly(phthalaldehyde) (Chapter 5). Advisors/Committee Members: Moore, Jeffrey S (advisor), Moore, Jeffrey S (Committee Chair), Suslick, Kenneth S (committee member), Zimmerman, Steven (committee member), Guironnet, Damien (committee member).

Subjects/Keywords: Stimuli-responsive; Poly(olefin sulfone); Base degradable; Acid degradable; Polymersome; pH; Triggered release; Transient; Capsule; Encapsulation

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Casey, C. M. (2016). Design and development of stimuli-responsive materials: pH sensitive polymersomes and poly(olefin sulfone)s. (Doctoral Dissertation). University of Illinois – Urbana-Champaign. Retrieved from http://hdl.handle.net/2142/95548

Chicago Manual of Style (16^th Edition):

Casey, Catherine M. “Design and development of stimuli-responsive materials: pH sensitive polymersomes and poly(olefin sulfone)s.” 2016. Doctoral Dissertation, University of Illinois – Urbana-Champaign. Accessed January 23, 2021. http://hdl.handle.net/2142/95548.

MLA Handbook (7^th Edition):

Casey, Catherine M. “Design and development of stimuli-responsive materials: pH sensitive polymersomes and poly(olefin sulfone)s.” 2016. Web. 23 Jan 2021.

Vancouver:

Casey CM. Design and development of stimuli-responsive materials: pH sensitive polymersomes and poly(olefin sulfone)s. [Internet] [Doctoral dissertation]. University of Illinois – Urbana-Champaign; 2016. [cited 2021 Jan 23]. Available from: http://hdl.handle.net/2142/95548.

Council of Science Editors:

Casey CM. Design and development of stimuli-responsive materials: pH sensitive polymersomes and poly(olefin sulfone)s. [Doctoral Dissertation]. University of Illinois – Urbana-Champaign; 2016. Available from: http://hdl.handle.net/2142/95548

19. Degonville, Maximilien. Etude numérique de la dynamique sous écoulement de gouttes et vésicules avec viscosités de surface : Numerical study of the dynamics of droplets and vesicles with surface viscosities under flow.

Degree: Docteur es, Mécanique et physique des fluides, 2018, Aix Marseille Université

URL: http://www.theses.fr/2018AIXM0751

►

De nombreux systèmes fluides dans les domaines de la biologie ou encore de la cosmétique sont limités par une interface dont les propriétés mécaniques régissent… (more)

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De nombreux systèmes fluides dans les domaines de la biologie ou encore de la cosmétique sont limités par une interface dont les propriétés mécaniques régissent la stabilité. En particulier, les objets tels que des gouttes, vésicules ou polymersomes se déforment dans un écoulement simple et mènent à une grande richesse de dynamiques spatio-temporelles contrôlées par la nature des matériaux qui composent l'interface. Les travaux présentés concernent l'étude numérique de la déformation de ces objets dans un écoulement de Stokes, en particulier dans des situations où les viscosités de l'interface jouent un rôle important. Un code de calcul couplant intégrales de frontières et éléments finis a été utilisé afin de décrire la physique interfaciale et étudier leur comportement une fois plongés dans un écoulement. Ces travaux ont permis d'étudier l'influence des viscosités interfaciales sur la dynamique d'une goutte dans un écoulement extensionnel plan, leur influence sur sa dynamique de déformation et sur les conditions de rupture de celle-ci. Les études réalisées sur une vésicule fortement dégonflée et plongée dans un écoulement cisaillé ont caractérisé la bifurcation entre les deux familles de forme existantes dans ces conditions. Ces formes ayant une influence sur la dynamique de la vésicule dans l'écoulement, celle-ci a été étudiée dans le cadre d'un écoulement infini puis proche d'une paroi parallèle à l'écoulement. Enfin, de premiers résultats sur la dynamique d'un polymersome dans un écoulement cisaillé permettent de construire un diagramme de phase illustrant les différents comportement de cet objet en fonction de la viscosité de la membrane et du taux de cisaillement

There are many fluid systems in the biology, food industry, pharmacology or cosmestics fields that are bound by an interface which mechanical properties rule the system stability. Objects like droplets, vesicles or polymersomes change their shape in a simple flow which lead to a wealth of space and time dynamics. These properties are controlled by the nature of the interface material. The aim of this work is the numerical study of the deformation of droplets, vesicles and polymersomes in a Stokes flow, especially when the interfacial viscosities play an important role. A numerical computation code coupling boundary integrals and finite elements was used to describe the interfacial physics of these objects and study their behaviour when immerged in a flow. Multiple resolution strategies where developped to this end in order to optimize the numerical computation in the cas of an interface with viscosities.Using this work, the influence of interfacial viscosities on the dynamics of a droplet in an extensional flow is studied : in particular, their influence on the stretching dynamics of a droplet and its break up conditions was characterized. The study of a vesicle, droplet bounded by a lipid bilayer, strongly deflated and immerged in a shear flow detailed the bifurcation between two shape types existing for this system. These shapes have an influence…

Advisors/Committee Members: Leonetti, Marc (thesis director), Boëdec, Gwenn (thesis director).

Subjects/Keywords: Goutte; Simulation numérique; Viscosité interfaciale; Vésicule; Polymersome; Proche paroi; Vesicles; Droplets; Surface viscosity; Stokes flow; Numerical simula- tion; Boundary integral method

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Degonville, M. (2018). Etude numérique de la dynamique sous écoulement de gouttes et vésicules avec viscosités de surface : Numerical study of the dynamics of droplets and vesicles with surface viscosities under flow. (Doctoral Dissertation). Aix Marseille Université. Retrieved from http://www.theses.fr/2018AIXM0751

Chicago Manual of Style (16^th Edition):

Degonville, Maximilien. “Etude numérique de la dynamique sous écoulement de gouttes et vésicules avec viscosités de surface : Numerical study of the dynamics of droplets and vesicles with surface viscosities under flow.” 2018. Doctoral Dissertation, Aix Marseille Université. Accessed January 23, 2021. http://www.theses.fr/2018AIXM0751.

MLA Handbook (7^th Edition):

Degonville, Maximilien. “Etude numérique de la dynamique sous écoulement de gouttes et vésicules avec viscosités de surface : Numerical study of the dynamics of droplets and vesicles with surface viscosities under flow.” 2018. Web. 23 Jan 2021.

Vancouver:

Degonville M. Etude numérique de la dynamique sous écoulement de gouttes et vésicules avec viscosités de surface : Numerical study of the dynamics of droplets and vesicles with surface viscosities under flow. [Internet] [Doctoral dissertation]. Aix Marseille Université 2018. [cited 2021 Jan 23]. Available from: http://www.theses.fr/2018AIXM0751.

Council of Science Editors:

Degonville M. Etude numérique de la dynamique sous écoulement de gouttes et vésicules avec viscosités de surface : Numerical study of the dynamics of droplets and vesicles with surface viscosities under flow. [Doctoral Dissertation]. Aix Marseille Université 2018. Available from: http://www.theses.fr/2018AIXM0751

Duke University

20. Hofmann, Christina Lehmkuhl. Delivery of Myoglobin Polymersomes Results in Tumor Hemorrhagic Necrosis and Enhanced Radiation Response .

Degree: 2015, Duke University

URL: http://hdl.handle.net/10161/9843

► There is a critical need to target tumor hypoxia as patients with hypoxic tumors have worse prognosis due to aggressive phenotypes and resistance to… (more)

▼ There is a critical need to target tumor hypoxia as patients with hypoxic tumors have worse prognosis due to aggressive phenotypes and resistance to radiotherapy and chemotherapy. The overall goal of this work is to improve response to conventional cancer therapies by targeting tumor hypoxia. This has been carried out and evaluated through the use of polymersome-encapsulated myoglobin (PEMs) with the hypothesis that O2-releasing PEMs will increase tumor oxygenation, and thereby improve response to radiotherapy. Mb was chosen as an O2 carrying protein to deliver to tumors because it has a strong association to O2, providing a mechanism to deliver O2 only within the hypoxic regions of the tumor. Mb was loaded within nanoscale polymeric vesicles that were expected to accumulate within solid tumors due to the enhanced permeability and retention (EPR) effect. This hypothesis has been tested through the following aims: 1. Develop NIR imaging techniques for studying the biodistribution and pharmacokinetics of polymersomes 2. Establish the effects of Mb-containing polymersomes on tumor physiology 3. Modify tumor growth through delivery of Mb polymersomes in combination with a cytotoxic therapy specific to aerobic tumors These aims have been evaluated through numerous in vivo studies. First, polymersomes of various polymer formulations and diameters ranging from 110-550 nm were prepared with a near-infrared (NIR) -emissive fluorophore. Using live animal fluorescence imaging, I was able to study the biodistribution of the polymersomes following i.v. administration, demonstrating significant polymersome accumulation in orthotopic 4T1 mammary carcinomas. In addition, a novel method for measuring pharmacokinetics was developed, using serial small volume blood draws from individual mice. The plasma fluorescence in microcapillary tubes was used to quantify polymersome concentrations, demonstrating long circulation half-lives that varied from 6-23 h. Toxicity of various polymersome formulations were also studied in vitro and in vivo, revealing negligible toxicities. For the second aim, PEMs were administered i.v. in tumor-bearing mice. Unexpectedly, we observed a dramatic gross tumor effect within hours of treatment in both orthotopic 4T1 tumors and flank Renca renal cell carcinomas. Histological analysis revealed endothelial cell apoptosis as early as 1 h following treatment, with scattered tumor cell death throughout the tumor by 4 h. Hematoxylin and eosin staining showed significant necrosis 24 h following PEM treatment. Vascular effects and polymersome distribution were studied in 4T1 window chamber tumors. Following i.v. treatment with PEMs, intravital microscopy was used to image polymersome fluorescence, brightfield transmission was imaged for vessel morphology and blood flow, and a tunable filter was used for determining hemoglobin (Hb) oxygen saturation. Tumor hemorrhaging was observed within hours of PEM treatment, which was not seen with empty polymersomes. This was consistent with the… Advisors/Committee Members: Dewhirst, Mark W (advisor).

Subjects/Keywords: Biomedical engineering; Medical imaging and radiology; Oncology; myoglobin; near-infrared imaging; polymersome; radiation; tumor; vascular disrupting agent

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Hofmann, C. L. (2015). Delivery of Myoglobin Polymersomes Results in Tumor Hemorrhagic Necrosis and Enhanced Radiation Response . (Thesis). Duke University. Retrieved from http://hdl.handle.net/10161/9843

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Hofmann, Christina Lehmkuhl. “Delivery of Myoglobin Polymersomes Results in Tumor Hemorrhagic Necrosis and Enhanced Radiation Response .” 2015. Thesis, Duke University. Accessed January 23, 2021. http://hdl.handle.net/10161/9843.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Hofmann, Christina Lehmkuhl. “Delivery of Myoglobin Polymersomes Results in Tumor Hemorrhagic Necrosis and Enhanced Radiation Response .” 2015. Web. 23 Jan 2021.

Vancouver:

Hofmann CL. Delivery of Myoglobin Polymersomes Results in Tumor Hemorrhagic Necrosis and Enhanced Radiation Response . [Internet] [Thesis]. Duke University; 2015. [cited 2021 Jan 23]. Available from: http://hdl.handle.net/10161/9843.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Hofmann CL. Delivery of Myoglobin Polymersomes Results in Tumor Hemorrhagic Necrosis and Enhanced Radiation Response . [Thesis]. Duke University; 2015. Available from: http://hdl.handle.net/10161/9843

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Université de Bordeaux I

21. Bofinger, Robin. Photocontrolled biomimetic communication between molecules and nanosystems in confined compartments : Communication moléculaire biomimétique entre molécules et nanosystèmes photocontrôlés en compartiments confinés.

Degree: Docteur es, Chimie organique, 2013, Université de Bordeaux I

URL: http://www.theses.fr/2013BOR14933

►

Cette thèse se focalise sur la synthèse et l'étude de nouvelles molécules photoactives et leurs applications en tant que marqueurs, senseurs moléculaires et récepteurs d’ions… (more)

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Cette thèse se focalise sur la synthèse et l'étude de nouvelles molécules photoactives et leurs applications en tant que marqueurs, senseurs moléculaires et récepteurs d’ions photomodulables en milieux aqueux et organisés. Les fluorophores développés sont principalement des dérivés du bore-dipyrométhene (BODIPY), comportant des groupements réactifs (azoture, perfluorophényle), des chaines hydrophobes, ou sont intégrés à un récepteur de calcium biocompatible. Le développement d'architectures auto-assemblées multicompartimentées de type vésicules dans des polymersomes géant y est décrit. Ces architectures ont été utilisées pour la génération de lumière blanche dans un micro-domaine, et constitue un modèle pour l'étude de transfert d'ions calcium entre vésicules localisées dans des polymersomes individuels. Ce transfert entre nano-objets confinés à l'intérieur d'un polymersome géant représente un système prototype de communication cellulaire artificiel rudimentaire.

The thesis focuses on the study and design of novel photoactive molecules and their application as labeling agents, fluorescent molecular Ca2+-sensors and photolabile Ca2+-decaging agents in aqueous media and organized supramolecular assemblies. The designed fluorophores are based on boron-dipyrromethene (BODIPY) bearing hydrophobic chains or a reactive group like an azide or a perfluorophenyl moiety. Biocompatible calcium receptors have been prepared harnessing the fluorescence properties of BODIPY, naphthalimide and furan fluorophores. The development of self-assembled multicompartmentalized architectures, namely fluorocarbon vesicles in giant polymersomes is reported and the system has been used to create white light emission in confined microdomains. The Ca2+-based ion transfer ion the confined polymer compartments between individual fluorinated vesicles has been studied. The ion transfer in between vesicles in polymer microcompartments has been established as an artificial prototype system for cellular communication.

Advisors/Committee Members: McClenaghan, Nathan (thesis director).

Subjects/Keywords: Chimie supramoléculaire; Auto-assemblage; Photochimie; Multicompartimentation; Liposome; Polymèrsome; Récepteur d’ion; Fluorescence; Libération d’ion; Supramolecular chemistry; Self-assembly; Photochemistry; Multicompartmentalization; Liposome; Polymersome; Ion receptor; Fluorescence; Ion decaging

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Bofinger, R. (2013). Photocontrolled biomimetic communication between molecules and nanosystems in confined compartments : Communication moléculaire biomimétique entre molécules et nanosystèmes photocontrôlés en compartiments confinés. (Doctoral Dissertation). Université de Bordeaux I. Retrieved from http://www.theses.fr/2013BOR14933

Chicago Manual of Style (16^th Edition):

Bofinger, Robin. “Photocontrolled biomimetic communication between molecules and nanosystems in confined compartments : Communication moléculaire biomimétique entre molécules et nanosystèmes photocontrôlés en compartiments confinés.” 2013. Doctoral Dissertation, Université de Bordeaux I. Accessed January 23, 2021. http://www.theses.fr/2013BOR14933.

MLA Handbook (7^th Edition):

Bofinger, Robin. “Photocontrolled biomimetic communication between molecules and nanosystems in confined compartments : Communication moléculaire biomimétique entre molécules et nanosystèmes photocontrôlés en compartiments confinés.” 2013. Web. 23 Jan 2021.

Vancouver:

Bofinger R. Photocontrolled biomimetic communication between molecules and nanosystems in confined compartments : Communication moléculaire biomimétique entre molécules et nanosystèmes photocontrôlés en compartiments confinés. [Internet] [Doctoral dissertation]. Université de Bordeaux I; 2013. [cited 2021 Jan 23]. Available from: http://www.theses.fr/2013BOR14933.

Council of Science Editors:

Bofinger R. Photocontrolled biomimetic communication between molecules and nanosystems in confined compartments : Communication moléculaire biomimétique entre molécules et nanosystèmes photocontrôlés en compartiments confinés. [Doctoral Dissertation]. Université de Bordeaux I; 2013. Available from: http://www.theses.fr/2013BOR14933

University of Notre Dame

22. Dian Respati Arifin. Cellular Hemoglobin-Based Oxygen Carriers as Potential Artificial Blood Substitutes</h1>.

Degree: Chemical Engineering, 2005, University of Notre Dame

URL: https://curate.nd.edu/show/5h73pv65d0h

► The objective of this study was to develop an effective and reliable cellular hemoglobin-based blood substitute (HBOC). Cellular HBOCs were created by encapsulating bovine… (more)

▼ The objective of this study was to develop an effective and reliable cellular hemoglobin-based blood substitute (HBOC). Cellular HBOCs were created by encapsulating bovine hemoglobin (Hb) into the aqueous cores of liposomes, poly(ethylene glycol) (PEG) conjugated liposomes and polymersomes. In order to evaluate the potential of the dispersions as cellular HBOCs, the following physical properties were measured: vesicle size distribution, Hb encapsulation efficiency, oxygen binding property (as indicated by P50 and cooperativity coefficient), and encapsulated methemoglobin level. The oxygen binding properties of unmodified liposomes encapsulated Hb (ULEHs), PEGylated LEHs (PEG-LEHs) and polymersomes encapsulated Hb (PEHs) were comparable to human RBCs, indicating that these vesicles displayed good potential as cellular HBOCs. The physical integrity of ULEH dispersions in phosphate buffered saline at physiological pH and temperature was unstable post-production, due to osmosis of water into and out of the liposome core, which implies that ULEHs will be osmotically fragile in the blood stream. This potential problem was solved by grafting PEG molecules onto the liposome surface, thereby strengthening the liposome bilayers. However, the Hb encapsulation efficiency of PEG-LEH dispersions was low. The intravascular circulation, biocompatibility and colloidal state of PEG-LEH dispersions were limited due to the limited PEG surface coverage and molecular weight that can be stably conjugated onto the liposome surface. In contrast, PEH dispersions possessed higher Hb encapsulation efficiencies compared to ULEH, PEG-LEH, PEGylated actin-containing LEH, lipogel particle and nanoscale hydrogel particle dispersions loaded with Hb. Unlike LEH dispersions, encapsulation of Hb into polymersomes did not enhance Hb oxidation. Polymersomes possessed superior PEG shielding ability compared to PEG-liposomes, due to 100% PEG surface coverage with longer PEG brushes. Since polymersomes can be synthesized with thicker hydrophobic membranes compared to liposomes, polymersomes are mechanically stronger than liposomes. Simulation of in-vivo oxygen transport in a capillary and to surrounding tissues, demonstrated that PEH dispersions could be engineered for applications from routine surgery to treatment of trauma. We concluded that PEH dispersions were readily prepared and exhibited good potential as a cellular HBOC, while offering superior physical properties, which may alleviate the limitations encountered with current designs of cellular HBOCs. Advisors/Committee Members: Dr. David Leighton, Committee Member.

Subjects/Keywords: hemoglobin; polymersome; encapsulation efficiency; oxygen carrier; blood substitute; fractionation; liposome; light scattering

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Arifin, D. R. (2005). Cellular Hemoglobin-Based Oxygen Carriers as Potential Artificial Blood Substitutes</h1>. (Thesis). University of Notre Dame. Retrieved from https://curate.nd.edu/show/5h73pv65d0h

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Arifin, Dian Respati. “Cellular Hemoglobin-Based Oxygen Carriers as Potential Artificial Blood Substitutes</h1>.” 2005. Thesis, University of Notre Dame. Accessed January 23, 2021. https://curate.nd.edu/show/5h73pv65d0h.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Arifin, Dian Respati. “Cellular Hemoglobin-Based Oxygen Carriers as Potential Artificial Blood Substitutes</h1>.” 2005. Web. 23 Jan 2021.

Vancouver:

Arifin DR. Cellular Hemoglobin-Based Oxygen Carriers as Potential Artificial Blood Substitutes</h1>. [Internet] [Thesis]. University of Notre Dame; 2005. [cited 2021 Jan 23]. Available from: https://curate.nd.edu/show/5h73pv65d0h.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Arifin DR. Cellular Hemoglobin-Based Oxygen Carriers as Potential Artificial Blood Substitutes</h1>. [Thesis]. University of Notre Dame; 2005. Available from: https://curate.nd.edu/show/5h73pv65d0h

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Université de Lorraine

23. Ferji, Khalid. Synthèse contrôlée et auto-organisation de glycopolymères amphiphiles à greffons polymères mésogènes, destinés à la vectorisation de principes actifs : Controlled synthesis and self-assembly of amphiphilic glycopolymers with polymeric mesogen grafts, in view of drug delivery applications.

Degree: Docteur es, Génie des procédés et des produits, 2013, Université de Lorraine

URL: http://www.theses.fr/2013LORR0118

►

De nouveaux glycopolymères greffés aux paramètres macromoléculaires contrôlés [dextrane-g-poly(acrylate de diéthylène glycol cholestéryle), Dex-g-PADEGChol] ont été préparés en quatre étapes via la stratégie de synthèse… (more)

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De nouveaux glycopolymères greffés aux paramètres macromoléculaires contrôlés [dextrane-g-poly(acrylate de diéthylène glycol cholestéryle), Dex-g-PADEGChol] ont été préparés en quatre étapes via la stratégie de synthèse « grafting from». L'originalité de ces glycopolymères réside dans la combinaison, et pour la première fois, d'une dorsale polysaccharide hydrophile biocompatible/ biodégradable et de greffons polymères hydrophobes à caractère mésogène. L'ATRP a été utilisée pour contrôler la croissance des greffons PADEGChol en milieu homogène à partir d'un macroamorceur dérivé de dextrane (DexAcBr). Les conditions de polymérisation avaient été préalablement ajustées en étudiant l'homopolymérisation du monomère ADEGChol en présence d'un amorceur modèle et de plusieurs systèmes catalytiques CuIBr/(PMDETA ou OPMI) dans différents solvants (THF ou toluène). Le caractère amphiphile de ces glycopolymères a été évalué et leurs propriétés mésomorphes ont été étudiées par calorimétrie différentielle à balayage, microscopie optique à lumière polarisante et par diffraction des rayons X. Des études préliminaires par microscope électronique à transmission et diffusion dynamique de la lumière polarisée ont démontré que ces glycopolymères adoptent une morphologie vésiculaire appelée « polymersome » en phase aqueuse, lorsque le DMSO est utilisé comme co-solvant. Ces nano-objets pourront être testés ultérieurement pour la formulation d'un nouveau type de vecteurs de principes actifs

New graft glycopolymers with well-defined parameters [dextran-g-poly(diethylene glycol cholesteryl ether acrylate) (Dex-g-PADEGChol)] have been prepared in four steps using the "grafting from" strategy. Challenge of this work arises from the combination for the first time of a hydrophilic, biocompatible/biodegradable polysaccharide backbone with mesogen hydrophobic polymeric grafts. Controlled growth of the grafts (PADEGChol) was obtained using ATRP initiated in homogeneous medium from a dextran derivative (DexAcBr). In order to find the best polymerization conditions, homopolymerization of ADEGChol monomer was investigated using an initiator model and various catalytic systems CuIBr/(PMDETA or OPMI) in two solvents (Toluene and THF). The amphiphilic properties of such glycopolymers were evaluated and their mesomorphic properties have been studied by thermal polarizing optical microscopy, differential scanning calorimetry and X-ray scattering. Using transmission electron microscopy and dynamic light scattering, vesicular morphology called "polymersome" was observed in aqueous medium when DMSO was used as co-solvent. These polymersomes could be tested as new drug delivery systems

Advisors/Committee Members: Six, Jean-Luc (thesis director), Nouvel, Cécile (thesis director).

Subjects/Keywords: Glycopolymère; Grafting from; Polymérisation radicalaire par transfert d'atome (ATRP); Cholestérol; Dextrane; Dextrane-g-poly(acrylate de diéthylène glycol cholestéryle) (Dex-g-PADEGChol); Copolymère amphiphile; Tensio-actif; Cristal-liquide; Mésomorphe; Auto-assemblage; Polymersome; Vésicule; Vecteur de principe actif; Glycopolymer; Grafting from; Atom transfer radical polymerization (ATRP); Cholesterol; Dextran; Dextran-g-poly(diethylene glycol cholesteryl ether acrylate) (Dex-g-PADEGChol); Amphiphilic copolymer; Surfactant; Liquid-crystal; Mesomorphic; Self-assembly; Polymersome; Vesicle; Drug-delivery; 547.28; 668.9

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Ferji, K. (2013). Synthèse contrôlée et auto-organisation de glycopolymères amphiphiles à greffons polymères mésogènes, destinés à la vectorisation de principes actifs : Controlled synthesis and self-assembly of amphiphilic glycopolymers with polymeric mesogen grafts, in view of drug delivery applications. (Doctoral Dissertation). Université de Lorraine. Retrieved from http://www.theses.fr/2013LORR0118

Chicago Manual of Style (16^th Edition):

Ferji, Khalid. “Synthèse contrôlée et auto-organisation de glycopolymères amphiphiles à greffons polymères mésogènes, destinés à la vectorisation de principes actifs : Controlled synthesis and self-assembly of amphiphilic glycopolymers with polymeric mesogen grafts, in view of drug delivery applications.” 2013. Doctoral Dissertation, Université de Lorraine. Accessed January 23, 2021. http://www.theses.fr/2013LORR0118.

MLA Handbook (7^th Edition):

Ferji, Khalid. “Synthèse contrôlée et auto-organisation de glycopolymères amphiphiles à greffons polymères mésogènes, destinés à la vectorisation de principes actifs : Controlled synthesis and self-assembly of amphiphilic glycopolymers with polymeric mesogen grafts, in view of drug delivery applications.” 2013. Web. 23 Jan 2021.

Vancouver:

Ferji K. Synthèse contrôlée et auto-organisation de glycopolymères amphiphiles à greffons polymères mésogènes, destinés à la vectorisation de principes actifs : Controlled synthesis and self-assembly of amphiphilic glycopolymers with polymeric mesogen grafts, in view of drug delivery applications. [Internet] [Doctoral dissertation]. Université de Lorraine; 2013. [cited 2021 Jan 23]. Available from: http://www.theses.fr/2013LORR0118.

Council of Science Editors:

Ferji K. Synthèse contrôlée et auto-organisation de glycopolymères amphiphiles à greffons polymères mésogènes, destinés à la vectorisation de principes actifs : Controlled synthesis and self-assembly of amphiphilic glycopolymers with polymeric mesogen grafts, in view of drug delivery applications. [Doctoral Dissertation]. Université de Lorraine; 2013. Available from: http://www.theses.fr/2013LORR0118

Université de Bordeaux I

24. Sanson, Charles. Vésicules polymères biorésorbables et stimulables pour des applications en vectorisation : The evolutionary process of psychological contracts and meaning to the work : the case of a food-company.

Degree: Docteur es, Polymères, 2010, Université de Bordeaux I

URL: http://www.theses.fr/2010BOR13985

►

L’auto-assemblage de copolymères à blocs amphiphiles est un outil puissant de la chimie supramoléculaire pour la conception de nano-objets complexes et fonctionnels. Dans ces travaux… (more)

Subjects/Keywords: Copolymères à blocs; Polypeptide; Polycarbonate; Vésicules; Polymèrosome; Auto-assemblage; Nanoprécipitation; Biodégradable; Stimulable; Doxorubicine; Délivrance contrôlée; Maghémite; Block copolymer; Polypeptide; Polycarbonate; Vesicle; Polymersome; Self-assembly; Nanoprecipitation; Biodegradable; Stimuli-responsive; Doxorubicin; Drug delivery; Controlled release

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Sanson, C. (2010). Vésicules polymères biorésorbables et stimulables pour des applications en vectorisation : The evolutionary process of psychological contracts and meaning to the work : the case of a food-company. (Doctoral Dissertation). Université de Bordeaux I. Retrieved from http://www.theses.fr/2010BOR13985

Chicago Manual of Style (16^th Edition):

Sanson, Charles. “Vésicules polymères biorésorbables et stimulables pour des applications en vectorisation : The evolutionary process of psychological contracts and meaning to the work : the case of a food-company.” 2010. Doctoral Dissertation, Université de Bordeaux I. Accessed January 23, 2021. http://www.theses.fr/2010BOR13985.

MLA Handbook (7^th Edition):

Sanson, Charles. “Vésicules polymères biorésorbables et stimulables pour des applications en vectorisation : The evolutionary process of psychological contracts and meaning to the work : the case of a food-company.” 2010. Web. 23 Jan 2021.

Vancouver:

Sanson C. Vésicules polymères biorésorbables et stimulables pour des applications en vectorisation : The evolutionary process of psychological contracts and meaning to the work : the case of a food-company. [Internet] [Doctoral dissertation]. Université de Bordeaux I; 2010. [cited 2021 Jan 23]. Available from: http://www.theses.fr/2010BOR13985.

Council of Science Editors:

Sanson C. Vésicules polymères biorésorbables et stimulables pour des applications en vectorisation : The evolutionary process of psychological contracts and meaning to the work : the case of a food-company. [Doctoral Dissertation]. Université de Bordeaux I; 2010. Available from: http://www.theses.fr/2010BOR13985

University of Pennsylvania

25. Levine, Dalia H. POLYMERSOMES: MULTI-FUNCTIONAL TOOLS FOR IN VIVO CANCER THERANOSTIC APPLICATIONS.

Degree: 2010, University of Pennsylvania

URL: https://repository.upenn.edu/edissertations/405

► ABSTRACT POLYMERSOMES: MULTI-FUNCTIONAL TOOLS FOR IN VIVO CANCER THERANOSTIC APPLICATIONS Dalia Hope Levine Dr. Daniel A. Hammer Nanoparticles are currently being developed as delivery vehicles… (more)

▼ ABSTRACT POLYMERSOMES: MULTI-FUNCTIONAL TOOLS FOR IN VIVO CANCER THERANOSTIC APPLICATIONS Dalia Hope Levine Dr. Daniel A. Hammer Nanoparticles are currently being developed as delivery vehicles for therapeutic and contrast imaging agents. Polymersomes (mesoscopic polymer vesicles) possess a number of attractive biomaterial properties, including greater biocompatibility, prolonged circulation times, and increased mechanical stability, that make them ideal for these applications. The polymersome architecture, with its large hydrophilic reservoir and thick hydrophobic lamellar membrane, provides significant storage capacity for water soluble and insoluble substances. The primary thesis aims are to develop multi-functional polymersomes for combination therapeutic applications, as well as simultaneous therapeutic and diagnostic applications. These multi-functional vesicles are capable of simultaneously loading both therapeutic agents, such as doxorubicin and combretastatin, and optical imaging agents, such as porphyrin-based near infrared (NIR) fluorophores, into their hydrophobic and hydrophilic regions. Doxorubicin, an anti-neoplastic agent, was encapsulated into PEO-b-PCL polymersomes and its release was characterized in situ. In vitro and in vivo studies confirmed the therapeutic potential of doxorubicin loaded polymersomes. Furthermore, the in vitro therapeutic efficacy of polymersomes loaded with combretastatin, an anti-vascular agent, was established with and without co-doxorubicin loading. The co-encapsulation of DOX and combretastatin into polymeric vesicles, generates a multi-functional drug loaded polymersome with the potential to eliminate tumorigenic cells an endothelial cells, respectively. The use of near infrared (NIR) emissive porphyrin polymersomes, loaded with porphyrin, for biodistribution studies, to non-invasively track the location of the polymersomes in tumor bearing mice was demonstrated using a noninvasive small animal optical imaging instrument which detects NIR fluorescence signal. Passive accumulation of drug loaded NIR-emissive polymersomes in tumor tissues of mice, as well as other organs, was observed. The study findings suggest the potential utility of NIR-emissive porphyrin polymersome in clinical diagnostic applications. Furthermore, preliminary results utilizing drug loaded porphyrin polymersomes to retard tumor growth and monitor vesicle location suggest these vesicles may have great future clinical utility. The ability to load components into the polymersome membrane and core shows enormous promise for future dual modality polymersomes with potential to be nanostructured biomaterials for future theranostic applications which provide both therapy and diagnosis.

Subjects/Keywords: Polymersome; Polymer Vesicle; Doxorubicin; Combretastatin; Porphyrin; multi-functional; theranostic; Analytical, Diagnostic and Therapeutic Techniques and Equipment; Biomedical Engineering and Bioengineering; Chemical Engineering

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Levine, D. H. (2010). POLYMERSOMES: MULTI-FUNCTIONAL TOOLS FOR IN VIVO CANCER THERANOSTIC APPLICATIONS. (Thesis). University of Pennsylvania. Retrieved from https://repository.upenn.edu/edissertations/405

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Levine, Dalia H. “POLYMERSOMES: MULTI-FUNCTIONAL TOOLS FOR IN VIVO CANCER THERANOSTIC APPLICATIONS.” 2010. Thesis, University of Pennsylvania. Accessed January 23, 2021. https://repository.upenn.edu/edissertations/405.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Levine, Dalia H. “POLYMERSOMES: MULTI-FUNCTIONAL TOOLS FOR IN VIVO CANCER THERANOSTIC APPLICATIONS.” 2010. Web. 23 Jan 2021.

Vancouver:

Levine DH. POLYMERSOMES: MULTI-FUNCTIONAL TOOLS FOR IN VIVO CANCER THERANOSTIC APPLICATIONS. [Internet] [Thesis]. University of Pennsylvania; 2010. [cited 2021 Jan 23]. Available from: https://repository.upenn.edu/edissertations/405.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Levine DH. POLYMERSOMES: MULTI-FUNCTIONAL TOOLS FOR IN VIVO CANCER THERANOSTIC APPLICATIONS. [Thesis]. University of Pennsylvania; 2010. Available from: https://repository.upenn.edu/edissertations/405

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

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