subject:(Polymerase chain reaction)

University of Hong Kong

1. 尹章權; Wan, Cheung-kuen. Evaluation of real time PCR for quantitative detection of enterococci in coastal water.

Degree: Master of Medical Sciences, 2016, University of Hong Kong

► Introduction: In the past, various methods have been used for the quantification of faecal indicators in water samples. Currently, membrane filtration is widely used for… (more)

▼ Introduction: In the past, various methods have been used for the quantification of faecal indicators in water samples. Currently, membrane filtration is widely used for the enumeration of faecal indicators in water samples. However, the turnaround time for membrane filtration is slow, particularly under emergency conditions. Hence, a rapid, reliable, high throughput method is in demand. Real time PCR, also known as the quantitative polymerase chain reaction (qPCR), has been suggested as a tool to replace culture-based methods for water monitoring. Different faecal indicators such as Total coliforms (TC), Faecal coliforms (FC) and Faecal streptococci (FS) have been used to monitor the water quality. In Hong Kong, E. coli has been used to indicate faecal pollution level since 1987. Recently, Enterococci have been reported as a more stable and reliable faecal indicator in both marine and fresh water. Information on the correlation between E. coli and Enterococci in local context are few. Therefore, the aim of this study was to evaluate real time PCR for the quantitative detection of Enterococci in coastal water. The relationship between E. coli and Enterococci obtained from water samples collected from the Shing Mun River was also investigated. Results: In the present studies, a total of 81 water samples were collected from three locations (Tai Wai, Fo Tan and Tai Shui Hang) along the Shing Mun River from September 2014 to February 2015. Apart from the densities of E. coli determined using a culture-based method, the densities of Enterococci in the 81 water samples were simultaneously determined using culture-based and qPCR methods, respectively. The median value of E. coli in Tai Wai, Fo Tan and Tai Shui Hang were 3066, 2200 and 115, respectively. The median value of Enterococci in Tai Wai, Fo Tan and Tai Shui Hang were 690, 46 and 9 CFU/100 ml, respectively. The amounts of Enterococci in the Tai Wai samples were significantly higher than in the Fo Tan and Tai Shui Hang samples, respectively (p<0.001). Moreover, the results showed the overall correlation coefficient between these two faecal indicators was 0.64 (N=81). The results also showed that the amounts of Enterococci measured using qPCR were significantly higher than those measured using culture-based methods (p<0.001). Both methods showed that the amount of Enterococci in Tai Wai samples was significantly higher than that in the Fo Tan and Tai Shui Hang samples, respectively (p<0.005). The overall regression analysis in log-scale showed that these two methods had significant positive linear relationships, with a correlation coefficient (r) of 0.74. The turnaround time for qPCR method is shorter than that for culture-based methods. Conclusions: The present study showed that real-time PCR has potential to complement traditional culture methods for assessing faecal pollution in coastal waters. However, this molecular-based method has some limitations, such as high start-up cost and an unpredictable qPCR inhibition. Further studies are needed to…

Subjects/Keywords: Enterococcus; Polymerase chain reaction

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APA (6^th Edition):

尹章權; Wan, C. (2016). Evaluation of real time PCR for quantitative detection of enterococci in coastal water. (Masters Thesis). University of Hong Kong. Retrieved from http://hdl.handle.net/10722/227898

Chicago Manual of Style (16^th Edition):

尹章權; Wan, Cheung-kuen. “Evaluation of real time PCR for quantitative detection of enterococci in coastal water.” 2016. Masters Thesis, University of Hong Kong. Accessed February 19, 2020. http://hdl.handle.net/10722/227898.

MLA Handbook (7^th Edition):

尹章權; Wan, Cheung-kuen. “Evaluation of real time PCR for quantitative detection of enterococci in coastal water.” 2016. Web. 19 Feb 2020.

Vancouver:

尹章權; Wan C. Evaluation of real time PCR for quantitative detection of enterococci in coastal water. [Internet] [Masters thesis]. University of Hong Kong; 2016. [cited 2020 Feb 19]. Available from: http://hdl.handle.net/10722/227898.

Council of Science Editors:

尹章權; Wan C. Evaluation of real time PCR for quantitative detection of enterococci in coastal water. [Masters Thesis]. University of Hong Kong; 2016. Available from: http://hdl.handle.net/10722/227898

Louisiana State University

2. Duhon, Lauren E. In vitro and in vivo evaluation of a Brucella putative hemagglutinin.

Degree: PhD, Veterinary Pathology and Pathobiology, 2009, Louisiana State University

URL: etd-01272010-205801 ; https://digitalcommons.lsu.edu/gradschool_dissertations/2610

► Brucellosis, a zoonotic disease caused by Brucella spp., presents both health and economic difficulties for livestock, wildlife, and humans. While brucellosis is nearly eradicated in… (more)

▼ Brucellosis, a zoonotic disease caused by Brucella spp., presents both health and economic difficulties for livestock, wildlife, and humans. While brucellosis is nearly eradicated in the United States, the disease remains detrimental in many countries worldwide. Attempts to produce a safe and effective small ruminant vaccine have been met with limited success. The current vaccine for bovine brucellosis in the United States is B. abortus RB51. This strain transiently colonizes the host and induces a cell-mediated immune response. Levels of protection have not been demonstrated in goats and thus it is considered a relatively poor caprine vaccine that probably does not survive long enough in the tissues to produce a sufficient protective immune response. This study analyzes the possibility of using RB51 containing plasmid QAE for vaccination in goats. The plasmid QAE contains a gene region of DNA from B. melitensis 16M hypothesized to encode for a putative hemagglutinin that is believed to be a host specificity protein. The region E gene sequence is not present in any strain of B. abortus, including RB51. It is proposed that the addition of region E to RB51 will enhance survivability in the caprine host to the extent where an adequate protective immune response is elicited. The QAE plasmid was electroporated into B. abortus RB51 and screened using ampicillin resistance as a selective measure. Ten goats were conjunctivally inoculated with RB51-QAE, the modified strain, and ten goats received strain RB51 in the same manner as controls. Two goats from each group were euthanized and necropsied at weekly intervals for four weeks and again at 42 days. Tissues from these animals were taken at necropsy and used to collect data for colonization and histopathology. Serum samples were also analyzed for Brucella-specific antibodies. Both strains transiently colonized the hosts without producing any detrimental pathology. However, the RB51-QAE goats demonstrated higher levels of colonization and greater humoral immune responses for longer periods of time. These are very promising findings as the levels of colonization and humoral responses may correspond with better protection. These results warrant further testing of RB51-QAE as a potential vaccine for caprine brucellosis.The putative hemagglutinin was characterized using hemagglutination assays, absorption assays, and mass spectrometry analysis following 2D gel electrophoresis. The region E protein was found to provide RB51-QAE with increased hemagglutinating and immunogenic capabilities. Furthermore, there is evidence to suggest that the protein is a cell surface protein, not excreted from the cell. The accepted gold standard for the detection of brucellae is bacterial culture. PCR is being evaluated as a possible alternative detection method for Brucella spp. in bodily fluids such as blood and urine. Currently, there is no accepted standard for detection of brucellae using PCR. The effects of template preparation, primer selection, and PCR optimization on the limit of detection…

Subjects/Keywords: Polymerase Chain Reaction; Hemagglutinin; Brucella

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APA (6^th Edition):

Duhon, L. E. (2009). In vitro and in vivo evaluation of a Brucella putative hemagglutinin. (Doctoral Dissertation). Louisiana State University. Retrieved from etd-01272010-205801 ; https://digitalcommons.lsu.edu/gradschool_dissertations/2610

Chicago Manual of Style (16^th Edition):

Duhon, Lauren E. “In vitro and in vivo evaluation of a Brucella putative hemagglutinin.” 2009. Doctoral Dissertation, Louisiana State University. Accessed February 19, 2020. etd-01272010-205801 ; https://digitalcommons.lsu.edu/gradschool_dissertations/2610.

MLA Handbook (7^th Edition):

Duhon, Lauren E. “In vitro and in vivo evaluation of a Brucella putative hemagglutinin.” 2009. Web. 19 Feb 2020.

Vancouver:

Duhon LE. In vitro and in vivo evaluation of a Brucella putative hemagglutinin. [Internet] [Doctoral dissertation]. Louisiana State University; 2009. [cited 2020 Feb 19]. Available from: etd-01272010-205801 ; https://digitalcommons.lsu.edu/gradschool_dissertations/2610.

Council of Science Editors:

Duhon LE. In vitro and in vivo evaluation of a Brucella putative hemagglutinin. [Doctoral Dissertation]. Louisiana State University; 2009. Available from: etd-01272010-205801 ; https://digitalcommons.lsu.edu/gradschool_dissertations/2610

University of Waikato

3. Anderson, Michael Andrew. Detection of Toxoplasma Gondii in Fresh New Zealand Farmed Meat by the Polymerase Chain Reaction .

Degree: 2015, University of Waikato

URL: http://hdl.handle.net/10289/10118

► Toxoplasma gondii is an obligate intracellular parasite that is estimated to infect one third of the world’s human population. Upon infection this parasite causes the… (more)

▼ Toxoplasma gondii is an obligate intracellular parasite that is estimated to infect one third of the world’s human population. Upon infection this parasite causes the disease toxoplasmosis which in most healthy humans is chronic and often asymptomatic. If infection occurs in a human host without a competent immune system such as an unborn baby or infant or someone suffering from AIDS, toxoplasmosis can lead to permanent disability or death. A growing amount of evidence indicates a statistical relationship between chronic infection in healthy adults and neurological disorders leading to behavioural and psychological problems. Toxoplasma gondii can be spread through contact with cat faeces. This was thought to be the main mode of human infection. Subsequent testing with murine and Felidae models has shown that the bradyzoite of Toxoplasma gondii within intracellular cysts in muscle and neural tissue are far more infectious than the oocyst morphology shed in cat faeces. This indicates that consumption of infected meat products may also play a major contributing role in the world’s high rate of toxoplasmosis infection. The chief aim of this study was to develop a sensitive PCR assay to detect the presence of Toxoplasma gondii in fresh raw meat products. Beef, chicken, lamb and pork mince were chosen as convenient forms of fresh raw meat as each pack available for purchase contains the meat from several animal’s tissues (increasing the likelihood of contamination) and it has been partially homogenised allowing for easier sampling and DNA extraction. Secondary aims of this study were to evaluate PCR primer sets targeting polymorphic regions to gain some understanding of what strains of Toxoplasma gondii are present in our meat and the prevalence in the local cat populations. To accomplish these aims positive and negative PCR controls were also developed using a live toxoplasmosis vaccine and DNA extracted from Zebrafish. DNA extraction techniques were developed to purify DNA from both muscle tissue and feline faecal samples and primer sets were selected from published literature as well as designed using NCBI primer BLAST. Toxoplasma gondii specific DNA was detected in only one of the 25 meat samples purchased. This was achieved using a primer set targeting the P30 region of the SAG1 gene and confirmed by DNA sequencing. Using primer sets targeting polymorphic sites within the Toxoplasma gondii genome, sequencing and restriction enzyme digests it was shown that Toxoplasma gondii present in the feral Felis catus population in the raglan area is type II at the GRA6 and SAG3 loci. Amplification of polymorphic PCR targets from DNA extracted from meat samples could not be achieved. Non-specific amplification occurred with a majority of primer sets trialed with meat DNA extracts indicating improvements are needed in primer design for the purpose of PCR detection of Toxoplasma gondii in meat. Nested PCR increased specificity however resulted in contamination in several instances. Advisors/Committee Members: Cursons, Raymond T (advisor), Peters, Linda (advisor).

Subjects/Keywords: Toxoplasma; Polymerase Chain Reaction

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APA (6^th Edition):

Anderson, M. A. (2015). Detection of Toxoplasma Gondii in Fresh New Zealand Farmed Meat by the Polymerase Chain Reaction . (Masters Thesis). University of Waikato. Retrieved from http://hdl.handle.net/10289/10118

Chicago Manual of Style (16^th Edition):

Anderson, Michael Andrew. “Detection of Toxoplasma Gondii in Fresh New Zealand Farmed Meat by the Polymerase Chain Reaction .” 2015. Masters Thesis, University of Waikato. Accessed February 19, 2020. http://hdl.handle.net/10289/10118.

MLA Handbook (7^th Edition):

Anderson, Michael Andrew. “Detection of Toxoplasma Gondii in Fresh New Zealand Farmed Meat by the Polymerase Chain Reaction .” 2015. Web. 19 Feb 2020.

Vancouver:

Anderson MA. Detection of Toxoplasma Gondii in Fresh New Zealand Farmed Meat by the Polymerase Chain Reaction . [Internet] [Masters thesis]. University of Waikato; 2015. [cited 2020 Feb 19]. Available from: http://hdl.handle.net/10289/10118.

Council of Science Editors:

Anderson MA. Detection of Toxoplasma Gondii in Fresh New Zealand Farmed Meat by the Polymerase Chain Reaction . [Masters Thesis]. University of Waikato; 2015. Available from: http://hdl.handle.net/10289/10118

Indian Institute of Science

4. Mondal, Sudip. Development And Optimization Of A Microchip PCR System Using Fluorescence Detection.

Degree: 2007, Indian Institute of Science

URL: http://hdl.handle.net/2005/1073

► Microfabricated thermal cyclers for nucleic acid amplification by using polymerase chain reaction (PCR) have been demonstrated by several groups over the last decade, with improved… (more)

▼ Microfabricated thermal cyclers for nucleic acid amplification by using polymerase chain reaction (PCR) have been demonstrated by several groups over the last decade, with improved cycling speed and smaller volumes when compared to conventional bench-top cyclers. However, high fabrication costs coupled with difficulties in temperature sensing and control remain impediments to commercialization. In this study we have used a silicon-glass device that takes advantage of the high thermal conductivity of silicon but at the same time utilizes minimum number of fabrication steps to make it suitable for disposable applications. The thermal cycler is based on noncontact induction heating developed in this group. The microchip reaction kinetics is studied for the first time in-situ during PCR, using a real-time fluorescence block that is capable of data acquisition every 0.7 s from the microchip. The fluorescence information from SYBR green I dye is used to optimize microchip amplification reactions and confirm the product by melting curve analysis. We have also developed a novel non-contact temperature sensing technique using SYBR green fluorescence that can be used for miniaturized PCR devices. The thesis is organized into the following chapters. In chapter 1 we introduce the basic biology ideas that are required to understand DNA amplification. DNA based analysis requires amplification of low initial concentrations to above detectable limits using a technique known as polymerase chain reaction (PCR). In this process, the sample is cycled through three thermal steps for 3040 times to produce multiple copies of DNA. In microchip PCR, conventional polypropylene tubes using 2050 µL volume are replaced by miniaturized devices using ~1 µL sample volumes. The device response improves in terms of ramp rate and total analysis time due to the small volume and smart design of the materials. In this chapter we summarize some of the issues important for miniaturized PCR devices and compare them with commercial tube PCR systems. In chapter 2 we describe the induction heating technique that was developed by our group for miniaturized devices. Induction heating is a noncontact heating technique unlike resistive heating which has been commonly used for microchip PCR. Though resistive heating is very efficient in terms of heat transfer efficiency, it is not suitable for disposable devices and requires multi-step microfabrication. Other non-contact heating techniques such as hot air and IR heating require larger size arrangements that are not suitable for miniaturized devices. The heating was verified by using a thermocouple soldered at the back of the secondary plate that was also used for feedback to the comparator circuit for control. The simple on-off circuit was able to control within ±0.1 ◦C with heating and cooling ramp rates of 25 ◦C/s and 2.5 ◦C/s respectively. In this chapter, we also describe the design and fabrication of the silicon-glass microchip fabricated in our lab. We have used silicon-glass hybrid device for PCR in which… Advisors/Committee Members: Venkataraman, V.

Subjects/Keywords: Polymerase Chain Reaction (PCR); Fluorescence; Microchip; Microchip Polymerase Chain Reaction; Real-time Polymerase Chain Reaction; Induction Heater; Microchip PCR; Biophysics

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APA (6^th Edition):

Mondal, S. (2007). Development And Optimization Of A Microchip PCR System Using Fluorescence Detection. (Thesis). Indian Institute of Science. Retrieved from http://hdl.handle.net/2005/1073

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Mondal, Sudip. “Development And Optimization Of A Microchip PCR System Using Fluorescence Detection.” 2007. Thesis, Indian Institute of Science. Accessed February 19, 2020. http://hdl.handle.net/2005/1073.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Mondal, Sudip. “Development And Optimization Of A Microchip PCR System Using Fluorescence Detection.” 2007. Web. 19 Feb 2020.

Vancouver:

Mondal S. Development And Optimization Of A Microchip PCR System Using Fluorescence Detection. [Internet] [Thesis]. Indian Institute of Science; 2007. [cited 2020 Feb 19]. Available from: http://hdl.handle.net/2005/1073.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Mondal S. Development And Optimization Of A Microchip PCR System Using Fluorescence Detection. [Thesis]. Indian Institute of Science; 2007. Available from: http://hdl.handle.net/2005/1073

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Georgia

5. Henderson, Terri Lynn. Statistical modeling and analysis of the polymerase chain reaction.

Degree: MS, Statistics, 2002, University of Georgia

URL: http://purl.galileo.usg.edu/uga_etd/henderson_terri_l_200205_ms

► Polymerase chain reaction (PCR) is a popular method of detecting the presence of a target gene. A mathematical model is developed based upon the principals… (more)

Subjects/Keywords: Polymerase Chain Reaction

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APA (6^th Edition):

Henderson, T. L. (2002). Statistical modeling and analysis of the polymerase chain reaction. (Masters Thesis). University of Georgia. Retrieved from http://purl.galileo.usg.edu/uga_etd/henderson_terri_l_200205_ms

Chicago Manual of Style (16^th Edition):

Henderson, Terri Lynn. “Statistical modeling and analysis of the polymerase chain reaction.” 2002. Masters Thesis, University of Georgia. Accessed February 19, 2020. http://purl.galileo.usg.edu/uga_etd/henderson_terri_l_200205_ms.

MLA Handbook (7^th Edition):

Henderson, Terri Lynn. “Statistical modeling and analysis of the polymerase chain reaction.” 2002. Web. 19 Feb 2020.

Vancouver:

Henderson TL. Statistical modeling and analysis of the polymerase chain reaction. [Internet] [Masters thesis]. University of Georgia; 2002. [cited 2020 Feb 19]. Available from: http://purl.galileo.usg.edu/uga_etd/henderson_terri_l_200205_ms.

Council of Science Editors:

Henderson TL. Statistical modeling and analysis of the polymerase chain reaction. [Masters Thesis]. University of Georgia; 2002. Available from: http://purl.galileo.usg.edu/uga_etd/henderson_terri_l_200205_ms

Oregon State University

6. Xu, Hong, 1968-. Development of PCR-based markers for identifying grape rootstocks.

Degree: MS, Genetics, 1995, Oregon State University

URL: http://hdl.handle.net/1957/34994

► Sequence-specific PCR markers were derived from one new and eight previously identified random amplified polymorphic DNA (RAPD) markers for the purpose of identifying grape (Vitis)… (more)

Subjects/Keywords: Polymerase chain reaction

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APA (6^th Edition):

Xu, Hong, 1. (1995). Development of PCR-based markers for identifying grape rootstocks. (Masters Thesis). Oregon State University. Retrieved from http://hdl.handle.net/1957/34994

Chicago Manual of Style (16^th Edition):

Xu, Hong, 1968-. “Development of PCR-based markers for identifying grape rootstocks.” 1995. Masters Thesis, Oregon State University. Accessed February 19, 2020. http://hdl.handle.net/1957/34994.

MLA Handbook (7^th Edition):

Xu, Hong, 1968-. “Development of PCR-based markers for identifying grape rootstocks.” 1995. Web. 19 Feb 2020.

Vancouver:

Xu, Hong 1. Development of PCR-based markers for identifying grape rootstocks. [Internet] [Masters thesis]. Oregon State University; 1995. [cited 2020 Feb 19]. Available from: http://hdl.handle.net/1957/34994.

Council of Science Editors:

Xu, Hong 1. Development of PCR-based markers for identifying grape rootstocks. [Masters Thesis]. Oregon State University; 1995. Available from: http://hdl.handle.net/1957/34994

University of Hong Kong

7. 葉珮瑋; Yip, Pui-wai. Detection of influenza A virus by MultiCode based real-time RT-PCR.

Degree: Master of Medical Sciences, 2017, University of Hong Kong

URL: http://hdl.handle.net/10722/251334

► Influenza virus belongs to the Orthomyxoviridae virus family, which is enveloped and pleomorphic with size ranging from 80-120nm. Influenza virus has a negative-sense, single-stranded, segmented… (more)

▼ Influenza virus belongs to the Orthomyxoviridae virus family, which is enveloped and pleomorphic with size ranging from 80-120nm. Influenza virus has a negative-sense, single-stranded, segmented RNA genome. Influenza A virus (IAV) is the most significant and widespread virus of the group since it can infect a broad range of avian and mammalian species. Also, due to high mutation rates and frequent genetic reassortment of influenza A virus, antigenic drift and antigenic shift often occur. In some rare cases, an antigenic shift may cause large global or regional pandemic outbreaks. There are several methods to detect influenza A virus in the laboratory, which includes, viral culture, direct immunofluorescence assay (DIF), rapid antigen detection kit and reverse transcription-PCR (RT-PCR). Nowadays, RT-PCR is the most common method to detect influenza A viruses, which is more sensitive and specific comparing with other options. In addition, real-time RT-PCR has short turnaround time (TAT), and it can provide quantification of viral load and identification of different subtypes of influenza A virus. The aim of this study was to evaluate a probe-free based, MultiCode®-RTx assay towards the detection of M gene of influenza A virus, and the feasibility to differentiate different subtypes of influenza A virus by the M gene melting temperature (Tm). Total nucleic acids of 111 clinical specimens and six culture isolates of pH1N1pdm09 (A/415742/09/H1N1), H2N2 (A/Asia/57/3), H3N2 (A/4851970/14), H5N1 (A/Vietnam/3028/04), H7N9 (A/Anhui/1/2013) and H9N2 (A/HK/1073/99) were included in this study. First, the performance of the MultiCode®-RTx assay for detection of influenza A infection was compared with the reference test, Queen Mary Hospital in-house real-time RT-PCR assay. The overall concordance of both assays was analyzed by the Cohen’s kappa value. The limit of detection (LOD) according to HA subtypes was determined by using the culture isolates. The sensitivity of MultiCode®-RTx M gene assay for overall influenza A, H1 and H3 subtype from clinical specimens was 88.9%, 93.3% and 87.5% respectively. The specificity was 100% and the overall PPV and NPV was 100% and 87.3%. The Cohen’s kappa value was 0.874 which is considered very good agreement between MultiCode®-RTx assay and in-house RT-PCR. The LOD of M gene with H1, H2, H3, H5, H7 and H9 subtypes was 67.5, 575, 67.5, 6.75, 10 and 550 TCID50 respectively. The Tm of H1N1 (clinical specimens), H3N2 (clinical specimens), H1N1 (culture isolate), H2N2 (culture isolate), H3N2 (culture isolate), H5N1 (culture isolate), H7N9 (culture isolate) and H9N2 (culture isolate) was 83.2 °C (sd ± = 0.14), 82.9 °C (sd ± = 0.14), 83.3 °C (sd ± = 0.07), 84.3 °C (sd ± = 0.04), 82.8 °C (sd ± = 0.04), 84.0 °C (sd ± = 0.04), 83.2 °C (sd ± = 0.04) and 83.9 °C (sd ± = 0.11) respectively. The Tm of different HA subtypes found in this study could be categorized into two groups; one was the Tm around 83°C (sd ± = 0.09) which included H1, H3 and H7 HA subtypes. Another was the Tm around 84°C (sd ± =…

Subjects/Keywords: Influenza - Diagnosis; Polymerase chain reaction - Diagnostic use

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

葉珮瑋; Yip, P. (2017). Detection of influenza A virus by MultiCode based real-time RT-PCR. (Masters Thesis). University of Hong Kong. Retrieved from http://hdl.handle.net/10722/251334

Chicago Manual of Style (16^th Edition):

葉珮瑋; Yip, Pui-wai. “Detection of influenza A virus by MultiCode based real-time RT-PCR.” 2017. Masters Thesis, University of Hong Kong. Accessed February 19, 2020. http://hdl.handle.net/10722/251334.

MLA Handbook (7^th Edition):

葉珮瑋; Yip, Pui-wai. “Detection of influenza A virus by MultiCode based real-time RT-PCR.” 2017. Web. 19 Feb 2020.

Vancouver:

葉珮瑋; Yip P. Detection of influenza A virus by MultiCode based real-time RT-PCR. [Internet] [Masters thesis]. University of Hong Kong; 2017. [cited 2020 Feb 19]. Available from: http://hdl.handle.net/10722/251334.

Council of Science Editors:

葉珮瑋; Yip P. Detection of influenza A virus by MultiCode based real-time RT-PCR. [Masters Thesis]. University of Hong Kong; 2017. Available from: http://hdl.handle.net/10722/251334

University of Johannesburg

8. Foxcroft, Zyta Krystyna. Assessment of the accuracy of pre-natal rhesus D typing on amniotic fluid using the polymerase chain reaction technique.

Degree: 2014, University of Johannesburg

URL: http://hdl.handle.net/10210/10654

►

M.Tech. (Biomedical Technology)

Despite the introduction of prophylactic treatment for Rh negative females, Rhesus Haemolytic Disease of the Foetus and Newborn (HDN) remains a problem.… (more)

▼

M.Tech. (Biomedical Technology)

Despite the introduction of prophylactic treatment for Rh negative females, Rhesus Haemolytic Disease of the Foetus and Newborn (HDN) remains a problem. The serological diagnosis of this disease is mainly by maternal antibody identification and titration and the estimation of the optical density deviation (ODD) at 450 nanometers of the amniotic fluid. The correlation of these two results is not always good. The advent of molecular biology techniques such as the Polymerase Chain Reaction (PCR) and the sequencing of genes heralded the start of prenatal diagnosis of genetically inherited diseases and also enabled the prediction of the Rhesus group of the foetus. It would be advantageous to be able to predict with certainty the RhD status of a foetus suspected of having HDN without subjecting the mother and foetus to the risk of multiple invasive procedures such as Chorionic Villus Sampling (CVS) and Foetal Blood Sampling(FBS). The amniocentesis performed initially on a mother suspected of carrying an affected foetus would provide the sample necessary for the extraction of foetal DNA for prenatal Rh determination. Two PCR assays were used to determine the RhD group of the foetus: one using two primers amplifying a section ofIntron 4 and the other using four primers, two specific for Exon 7 and two specific for Exon 10 of the Rh gene. In 85.7% (18/21 cases) there was complete correlation between the molecular and the serological methods for RhD determination. One White foetus presented a unique profile, that of RhD negative in both molecular assays and RhD positive serologically. In the non-White group there were discrepancies between the two molecular methods as well as between the molecular and the serological methods used. This study shows that great care should be taken in the interpretation of RhD status prenatally using molecular biology techniques especially in the non-Caucasian population of South Africa in which there are many polymorphisrns in the Rhesus blood group system. For the moment, the results should be used in conjunction with serological results and clinical parameters for the diagnosis and treatment of Rh HDN.

Subjects/Keywords: Molecular biology; Rh factor; Polymerase chain reaction

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APA (6^th Edition):

Foxcroft, Z. K. (2014). Assessment of the accuracy of pre-natal rhesus D typing on amniotic fluid using the polymerase chain reaction technique. (Thesis). University of Johannesburg. Retrieved from http://hdl.handle.net/10210/10654

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Foxcroft, Zyta Krystyna. “Assessment of the accuracy of pre-natal rhesus D typing on amniotic fluid using the polymerase chain reaction technique.” 2014. Thesis, University of Johannesburg. Accessed February 19, 2020. http://hdl.handle.net/10210/10654.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Foxcroft, Zyta Krystyna. “Assessment of the accuracy of pre-natal rhesus D typing on amniotic fluid using the polymerase chain reaction technique.” 2014. Web. 19 Feb 2020.

Vancouver:

Foxcroft ZK. Assessment of the accuracy of pre-natal rhesus D typing on amniotic fluid using the polymerase chain reaction technique. [Internet] [Thesis]. University of Johannesburg; 2014. [cited 2020 Feb 19]. Available from: http://hdl.handle.net/10210/10654.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Foxcroft ZK. Assessment of the accuracy of pre-natal rhesus D typing on amniotic fluid using the polymerase chain reaction technique. [Thesis]. University of Johannesburg; 2014. Available from: http://hdl.handle.net/10210/10654

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Louisiana State University

9. Matherne, Brian William. Populations of Vibrio vulnificus and Vibrio parahaemolyticus in Breton Sound and Barataria Bay.

Degree: MS, Environmental Sciences, 2009, Louisiana State University

URL: etd-11132009-104544 ; https://digitalcommons.lsu.edu/gradschool_theses/1055

► Vibrio vulnificus and Vibrio parahaemolyticus are halophilic gram-negative bacteria that are found in warm coastal waters. These two species are the leading cause of… (more)

▼ Vibrio vulnificus and Vibrio parahaemolyticus are halophilic gram-negative bacteria that are found in warm coastal waters. These two species are the leading cause of fatal shellfish poisoning. The objective of this study was to determine the population dynamics of V. vulnificus and V. parahaemolyticus as impacted by temperature and salinity in Breton Sound and Barataria Bay of Louisiana. Water samples were collected from each water body along s salinity gradient transect monthly from September 2007 through July 2009. Furthermore, from March 2008 through July 2009 sediment and live oyster samples were collected exclusively from the Breton Sound. The population of V. vulnificus and V. parahaemolyticus was measured using the most probable number (MPN) method and plating method. The abundance of putative V. parahaemolyticus and V. vulnificus in Barataria Bay and Breton Sound waters was seasonally dominated by water temperature, but spatially controlled by salinity level. The plate counts indicated that V. vulnificus appeared less abundant in the Gulf waters during colder months than V. parahaemolyticus. Based on the laboratory testing, V. parahaemolyticus appeared to grow better overall at higher salinity levels. The average annual population of putative V. parahaemolyticus and V. vulnificus over all the sampling sites in Breton Sound was slightly higher than that in Barataria Bay. Like that in the waters of Breton Sound, the population of putative V. parahaemolyticus and V. vulnificus in Breton Sound sediments also followed a trend. This research has shown a clear picture of the dynamics of putative V. parahaemolyticus and V. vulnificus populations in Breton Sound Estuary and Barataria Bay. However, further work is needed to (1) continue the PCR confirmation of V. parahaemolyticus and V. vulnificus, and (2) carry out statistical analysis for the relationships between environmental parameters and V. vulnificus and V. parahaemolyticus populations. The work would eventually lead to the establishment of a statistical relationship between the Vibrio concentrations and environmental parameters, in particular salinity and temperature, under a range of tidal, wind, and freshwater input conditions, which could be used to quantify the temporal and spatial variations of the Vibrio distributions in response to the environmental parameters.

Subjects/Keywords: Water; Sediment; Oysters; Vibrio; Polymerase Chain Reaction

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Matherne, B. W. (2009). Populations of Vibrio vulnificus and Vibrio parahaemolyticus in Breton Sound and Barataria Bay. (Masters Thesis). Louisiana State University. Retrieved from etd-11132009-104544 ; https://digitalcommons.lsu.edu/gradschool_theses/1055

Chicago Manual of Style (16^th Edition):

Matherne, Brian William. “Populations of Vibrio vulnificus and Vibrio parahaemolyticus in Breton Sound and Barataria Bay.” 2009. Masters Thesis, Louisiana State University. Accessed February 19, 2020. etd-11132009-104544 ; https://digitalcommons.lsu.edu/gradschool_theses/1055.

MLA Handbook (7^th Edition):

Matherne, Brian William. “Populations of Vibrio vulnificus and Vibrio parahaemolyticus in Breton Sound and Barataria Bay.” 2009. Web. 19 Feb 2020.

Vancouver:

Matherne BW. Populations of Vibrio vulnificus and Vibrio parahaemolyticus in Breton Sound and Barataria Bay. [Internet] [Masters thesis]. Louisiana State University; 2009. [cited 2020 Feb 19]. Available from: etd-11132009-104544 ; https://digitalcommons.lsu.edu/gradschool_theses/1055.

Council of Science Editors:

Matherne BW. Populations of Vibrio vulnificus and Vibrio parahaemolyticus in Breton Sound and Barataria Bay. [Masters Thesis]. Louisiana State University; 2009. Available from: etd-11132009-104544 ; https://digitalcommons.lsu.edu/gradschool_theses/1055

Univerzitet u Beogradu

10. Strelić, Nataša J., 1964-. Značaj molekularne detekcije bakterija u proceni efikasnosti terapije Rajterovog sindroma.

Degree: Biološki fakultet, 2016, Univerzitet u Beogradu

URL: https://fedorabg.bg.ac.rs/fedora/get/o:10683/bdef:Content/get

Biologija - Molekularna biologija / Biology - Molecular biology

Nalaz bakterija u sinoviji i sinovijskoj tečnosti inflamiranog zgloba je pobudilo nadu da bi antibiotska terapija mogla biti terapija izbora kod bolesnika sa Rajterovim sindromom...

Advisors/Committee Members: Magić, Zvonko.

Subjects/Keywords: Reiter's syndrome; therapy; polymerase chain reaction

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Strelić, Nataša J., 1. (2016). Značaj molekularne detekcije bakterija u proceni efikasnosti terapije Rajterovog sindroma. (Thesis). Univerzitet u Beogradu. Retrieved from https://fedorabg.bg.ac.rs/fedora/get/o:10683/bdef:Content/get

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Strelić, Nataša J., 1964-. “Značaj molekularne detekcije bakterija u proceni efikasnosti terapije Rajterovog sindroma.” 2016. Thesis, Univerzitet u Beogradu. Accessed February 19, 2020. https://fedorabg.bg.ac.rs/fedora/get/o:10683/bdef:Content/get.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Strelić, Nataša J., 1964-. “Značaj molekularne detekcije bakterija u proceni efikasnosti terapije Rajterovog sindroma.” 2016. Web. 19 Feb 2020.

Vancouver:

Strelić, Nataša J. 1. Značaj molekularne detekcije bakterija u proceni efikasnosti terapije Rajterovog sindroma. [Internet] [Thesis]. Univerzitet u Beogradu; 2016. [cited 2020 Feb 19]. Available from: https://fedorabg.bg.ac.rs/fedora/get/o:10683/bdef:Content/get.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Strelić, Nataša J. 1. Značaj molekularne detekcije bakterija u proceni efikasnosti terapije Rajterovog sindroma. [Thesis]. Univerzitet u Beogradu; 2016. Available from: https://fedorabg.bg.ac.rs/fedora/get/o:10683/bdef:Content/get

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Tartu University

11. Kõressaar, Triinu. Improvement of PCR primer design for detection of prokaryotic species .

Degree: 2012, Tartu University

URL: http://hdl.handle.net/10062/25917

► Polümeraasi ahelreaktsioon ehk PCR on molekulaarbioloogia meetod, mis võimaldab paljundada spetsiifilist DNA lõiku. Protsess toimub tsükliliselt ja kätkeb endas järgmisi etappe: kaheahelalise DNA järjestuse (sihtmärkjärjestuse)… (more)

▼ Polümeraasi ahelreaktsioon ehk PCR on molekulaarbioloogia meetod, mis võimaldab paljundada spetsiifilist DNA lõiku. Protsess toimub tsükliliselt ja kätkeb endas järgmisi etappe: kaheahelalise DNA järjestuse (sihtmärkjärjestuse) sulatamist kõrgel temperatuuril, kahe spetsiifilise DNA järjestuse (PCRi praimeri) seondumist sihtmärkjärjestusele konkreetsest PCRi katsest sõltuval madalamal temperatuuril (ehk praimerite sulamistemperatuuril Tm) ning seondunud praimerite pikendamist vastavalt spetsiifilisele DNA lõigu järjestusele kindla valgulise ensüümi abil. Paljundatud DNA-d detekteeritakse, kas spetsiifiliselt geelil pikkuse järgi või reaalajas produkti paljundamise käigus tekkiva signaali abil. PCR võimaldab sel viisil tuvastada suvalisest DNA proovist (kliiniline-, veterinaar-, toidu-, keskkonnaproov jne) vaid kindlale liigile iseäralikku DNA järjestust ning seetõttu on tehnoloogia leidnud rakendust erinevates valdkondades erinevate liikide või tüvede tuvastamiseks. Üks olulisemaid eeldusi edukaks PCRi teostamiseks on täpsete ja tundlikke praimerite disainimine (PCRi praimeridisain). PCRi praimeridisain sisaldab endas erinevaid etappe muuhulgas sihtmärkjärjestuse välja valimist (nt kindla järjestuse valimist bakterigenoomist) ning PCRi praimerijärjestuste disaini. Käesolev töö ongi keskendunud PCRi praimeridisaini erinevate etappide parendamisele: laialdaselt kasutusel oleva PCRi praimeridisaini programmi Primer3-e poolt kasutatava praimerite sulamistemperatuuri Tm arvutamise valemi täiustamisele ning sellele, kuidas prokarüootseid liigispetsiifilisi kordusjärjestusi PCRi sihtmärkjärjestustena kasutada ning, millise effekti see PCRi tulemustele annab. Viimase osana sisaldab töö prokarüootsete liigispetsiifiliste kordusjärjestuste iseloomustamist 613 erinevas prokarüootses liigis. Antud töö tulemused aitavad kaasa uute ja paremate molekulaardiagnostika testide loomisele esiteks lihtsustades nende väljatöötamist tänu antud töös leitud ja kirjeldatud PCRi sihtmärkjärjestustele, teiseks tõstes nende töökindlust, kuna liigispetsiifilistele kordusjärjestustele disainitud praimerid on kõrgendatud tundlikkusega ning, kuna parendatud praimeridisaini-programm Primer3 võimaldab disainida praimereid, mis vastavad täpsemalt etteantud molekulaardiagnostika testide tingimustele .; Polymerase chain reaction or PCR is a method in molecular biology, which enables amplification of specific DNA regions. It is a cyclic process, that comprises of heat denaturation of double-stranded DNA (target sequence), hybridization of two short oligonucleotides (called PCR primers) to the denaturated target sequence at temperature specific to the PCR reaction (termed melting temperature Tm) and extension of hybridized primers by the enzyme DNA polymerase. The amplified DNA can be identified either specifically on electrophoresis gel by its product length or by monitoring the signal generated through activation of primer attached flourecent labels during the amplification in real-time. Thus, PCR enables identification of the DNA sequence of…

Subjects/Keywords: polümeraasahelreaktsioon; molekulaardiagnostika; polymerase chain reaction; molecular diagnostics

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Kõressaar, T. (2012). Improvement of PCR primer design for detection of prokaryotic species . (Thesis). Tartu University. Retrieved from http://hdl.handle.net/10062/25917

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Kõressaar, Triinu. “Improvement of PCR primer design for detection of prokaryotic species .” 2012. Thesis, Tartu University. Accessed February 19, 2020. http://hdl.handle.net/10062/25917.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Kõressaar, Triinu. “Improvement of PCR primer design for detection of prokaryotic species .” 2012. Web. 19 Feb 2020.

Vancouver:

Kõressaar T. Improvement of PCR primer design for detection of prokaryotic species . [Internet] [Thesis]. Tartu University; 2012. [cited 2020 Feb 19]. Available from: http://hdl.handle.net/10062/25917.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Kõressaar T. Improvement of PCR primer design for detection of prokaryotic species . [Thesis]. Tartu University; 2012. Available from: http://hdl.handle.net/10062/25917

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Florida

12. Gilbert, Brittany. Detection of West Nile Virus in Equids through Single-Cell Polymerase Chain Reaction.

Degree: 2013, University of Florida

URL: http://ufdc.ufl.edu/AA00057802

► The methods for detecting and analyzing West Nile Virus (WNV) in both equids and humans are limited to using relatively large amounts of sample and… (more)

▼ The methods for detecting and analyzing West Nile Virus (WNV) in both equids and humans are limited to using relatively large amounts of sample and have low sensitivity levels. This hinders both experimental and diagnostic sensitivity for such diseases that have low viremia levels, and is particularly detrimental for life-threatening diseases such as WNV. By using a nanowell chip, separation and analysis of tens of thousands of individual cells can overcome some of these problems. The present study aimed to develop a technique with which to identify WNV in nervous and non-nervous tissues on a single-cell scale. To conduct the single-cell polymerase chain reaction (PCR), infected and non-infected host vero cells were co-loaded onto a nanowell chip with PCR reaction mixture. The entire nanowell chip was then placed onto a thermal mastercycler with specific cycling conditions for one-step reverse transcription PCR (RT-PCR). After PCR, the nanowell chip was then imaged using a Nikon microscope for PCR product fluorescent detection of both G3PDH (an enzyme involved in glucose metabolism and other processes necessary for cellular life, which served as the internal control in this experiment) and WNV chimera. The intensities of each cell were recorded for further data analysis. To date, the undergraduate researcher has performed optimization of both primer sets and probe for G3PDH and WNV, optimization of cycling conditions, and optimization using a conventional real-time PCR machine as well as multiple negative and positive control tests. The full development of this technique is still underway, but results to date have been promising. Singleplex nanowell chips have produced strong fluorescence that was specific to the excitation filter on which it is imaged. The co-loaded, multiplex nanowell chip (both uninfected and infected cells as well as primers and probe for both WNV and G3PDH) has produced vivid PCR product fluorescence for both WNV and G3PDH at their respective wavelengths. ( en )

Subjects/Keywords: Chimeras; Fluorescence; Histograms; Microscopes; Polymerase chain reaction; Reverse transcriptase polymerase chain reaction; Signal detection; Signals; Vero cells; West Nile virus; Equidae; Polymerase chain reaction; West Nile virus

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Gilbert, B. (2013). Detection of West Nile Virus in Equids through Single-Cell Polymerase Chain Reaction. (Thesis). University of Florida. Retrieved from http://ufdc.ufl.edu/AA00057802

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Gilbert, Brittany. “Detection of West Nile Virus in Equids through Single-Cell Polymerase Chain Reaction.” 2013. Thesis, University of Florida. Accessed February 19, 2020. http://ufdc.ufl.edu/AA00057802.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Gilbert, Brittany. “Detection of West Nile Virus in Equids through Single-Cell Polymerase Chain Reaction.” 2013. Web. 19 Feb 2020.

Vancouver:

Gilbert B. Detection of West Nile Virus in Equids through Single-Cell Polymerase Chain Reaction. [Internet] [Thesis]. University of Florida; 2013. [cited 2020 Feb 19]. Available from: http://ufdc.ufl.edu/AA00057802.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Gilbert B. Detection of West Nile Virus in Equids through Single-Cell Polymerase Chain Reaction. [Thesis]. University of Florida; 2013. Available from: http://ufdc.ufl.edu/AA00057802

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Florida

13. Prakoso, Dhani. Comparative Efficiency of Different Real-Time PCR Platforms and Chemistries for the Detection of Animal, Avian, and Human Influenzas.

Degree: MS, Veterinary Medical Sciences - Veterinary Medicine, 2011, University of Florida

URL: http://ufdc.ufl.edu/UFE0043771

► The goal of this research was to determine if PCR protocols can be standardized between laboratories for influenza testing. First, 25 influenza viruses, consisting of… (more)

Subjects/Keywords: Horses; Human influenza; Influenza; Orthomyxoviridae; Pandemics; Polymerase chain reaction; Reverse transcriptase polymerase chain reaction; RNA; Swine; Viruses; influenza – pcr – realtimepcr – swine

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Prakoso, D. (2011). Comparative Efficiency of Different Real-Time PCR Platforms and Chemistries for the Detection of Animal, Avian, and Human Influenzas. (Masters Thesis). University of Florida. Retrieved from http://ufdc.ufl.edu/UFE0043771

Chicago Manual of Style (16^th Edition):

Prakoso, Dhani. “Comparative Efficiency of Different Real-Time PCR Platforms and Chemistries for the Detection of Animal, Avian, and Human Influenzas.” 2011. Masters Thesis, University of Florida. Accessed February 19, 2020. http://ufdc.ufl.edu/UFE0043771.

MLA Handbook (7^th Edition):

Prakoso, Dhani. “Comparative Efficiency of Different Real-Time PCR Platforms and Chemistries for the Detection of Animal, Avian, and Human Influenzas.” 2011. Web. 19 Feb 2020.

Vancouver:

Prakoso D. Comparative Efficiency of Different Real-Time PCR Platforms and Chemistries for the Detection of Animal, Avian, and Human Influenzas. [Internet] [Masters thesis]. University of Florida; 2011. [cited 2020 Feb 19]. Available from: http://ufdc.ufl.edu/UFE0043771.

Council of Science Editors:

Prakoso D. Comparative Efficiency of Different Real-Time PCR Platforms and Chemistries for the Detection of Animal, Avian, and Human Influenzas. [Masters Thesis]. University of Florida; 2011. Available from: http://ufdc.ufl.edu/UFE0043771

University of the Western Cape

14. Jacobs, Gwynneth. Evaluation of insertion-deletion polymorphisms with the kit Qiagen Investigator® DIPplex for forensic application in South Africa .

Degree: 2015, University of the Western Cape

URL: http://hdl.handle.net/11394/4690

► Insertion-deletion polymorphisms (indels) have been underutilized in forensic identification of individuals in comparison with single nucleotide polymorphisms (SNPs) and short tandem repeat (STRs) systems. The… (more)

▼ Insertion-deletion polymorphisms (indels) have been underutilized in forensic identification of individuals in comparison with single nucleotide polymorphisms (SNPs) and short tandem repeat (STRs) systems. The use of indels for the purpose of human identification is more advantageous than previously used methods as it combines desirable characteristics of both the SNPs and STRs i.e. low costs and simplistic typing methods as well as indels having small amplicons size, making them suitable for genotyping highly degraded DNA. Currently there is only one commercial kit available for the forensic community, the Investigator® DIPlex kit (Qiagen), which cover a total of 30 indel loci distributed over 19 autosomal chromosomes. The objective of this study was to evaluate the Qiagen Investigator® DIPplex kit for forensic application in South Africa. The kit‘s performance was evaluated by comparing different extraction methods; sensitivity, robustness and reproducibility were evaluated and forensic parameters (match probability, power of discrimination, polymorphism information content, power of exclusion and typical paternity index) were estimated based on population data generated from five South African populations (Afrikaner, Mixed Ancestry, Indian-Asian, Xhosa and Zulu). Population comparisons were performed using Fst-analysis, factorial component analysis as well as phylogenetic tree construction. DNA was extracted from buccal swabs and whole blood collected from a total of 512 individuals from the five South African population groups and genotyped using the Qiagen Investigator® DIPplex kit. Sanger DNA sequencing and sequence alignments confirmed the presence of a null allele at locus HLD97 which was present in high frequency in the Xhosa and Zulu populations. This observation was made in 14 individuals belonging to the Xhosa and Zulu populations. Null allele frequencies in all five South African populations were also estimated. Null alleles were estimated for all loci using analytical methods i.e. Charkraborty null allele estimator, Brookfield null allele estimators 1 and 2 and ML-NullFreq software program. The kit performed well in the laboratory, not requiring any additional reagents or instrumentation and successfully generating profiles with input DNA amounts as low as 0.2 ng/μL. Although well suited for forensic application, the Qiagen Investigator® DIPplex kit showed some drawbacks with regards to application on South African populations. The presence of a null allele at the HLD97 locus as well as indication of population substructure affects allele frequency estimates for the South African populations. Correction for population substructure as present within the South African populations should be considered using FST analysis and it is recommended that the HLD97 locus should be excluded from any kinship analysis performed on South African populations. Advisors/Committee Members: D‘Amato, Maria Eugenia (advisor), Davison, Sean (advisor).

Subjects/Keywords: Forensics; Genotyping; Polymerase Chain Reaction (PCR); Population genetics

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Jacobs, G. (2015). Evaluation of insertion-deletion polymorphisms with the kit Qiagen Investigator® DIPplex for forensic application in South Africa . (Thesis). University of the Western Cape. Retrieved from http://hdl.handle.net/11394/4690

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Jacobs, Gwynneth. “Evaluation of insertion-deletion polymorphisms with the kit Qiagen Investigator® DIPplex for forensic application in South Africa .” 2015. Thesis, University of the Western Cape. Accessed February 19, 2020. http://hdl.handle.net/11394/4690.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Jacobs, Gwynneth. “Evaluation of insertion-deletion polymorphisms with the kit Qiagen Investigator® DIPplex for forensic application in South Africa .” 2015. Web. 19 Feb 2020.

Vancouver:

Jacobs G. Evaluation of insertion-deletion polymorphisms with the kit Qiagen Investigator® DIPplex for forensic application in South Africa . [Internet] [Thesis]. University of the Western Cape; 2015. [cited 2020 Feb 19]. Available from: http://hdl.handle.net/11394/4690.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Jacobs G. Evaluation of insertion-deletion polymorphisms with the kit Qiagen Investigator® DIPplex for forensic application in South Africa . [Thesis]. University of the Western Cape; 2015. Available from: http://hdl.handle.net/11394/4690

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of the Western Cape

15. Tau, Tiroyamodimo. A forensic analysis of genetic variation in the Botswana population .

Degree: 2016, University of the Western Cape

URL: http://hdl.handle.net/11394/5657

► This thesis has been placed under a long term embargo. Forensic and population genetic parameters were investigated in the Botswana population using autosomal and Y-chromosome… (more)

▼ This thesis has been placed under a long term embargo. Forensic and population genetic parameters were investigated in the Botswana population using autosomal and Y-chromosome short tandem repeat markers. AmpFlSTR Profiler plus markers were used to investigate the genetic diversity and forensic parameters in 773 individuals from Botswana from the reference database of the Botswana Police. The levels of polymorphism found using the AmpFlSTR Profiler Plus markers showed that the nine loci that make up the AmpFlSTR Profiler Plus can differentiate individuals for forensic casework in the Botswana population. AmpFlSTR Identifiler autosomal STR markers were used to investigate the population structure according to ethno-linguistics and geography 990 individuals from Botswana that serve as a reference database for the Botswana Police. Using pairwise genetic distances (Fst), analysis of molecular variance (AMOVA), factorial correspondence analysis (FCA), and the unsupervised Bayesian clustering method found in STRUCTURE and the landscape genetics software TESS, ethno-linguistics were found to have a greater influence on population structure than geography. The patterns of population structure found using these markers highlight the need for regional reference databases that include both ethnolinguistic and geographic location information. These markers have important potential for bio-anthropological studies as well as for forensic applications. The 17 Y-chromosomal short tandem repeats found in AmpFlSTR Y-filer and a highly discriminatory Y-STR genotyping system (the Y-STR 10-plex developed in the Forensics DNA Laboratory at the University of the Western Cape) were analysed in 249 unrelated male individuals from Botswana. Rst, multi-dimensional scaling (MDS) and AMOVA were used to investigate population differentiation in Botswana. The discrimination capacity (DC) was found to be higher using the Y-STR 10-plex as compared to the 17 markers in the Y-filer genotyping system. No geographic regional or ethnic differentiation was observed between the Northern and Southern regions of Botswana using both marker systems. Regional and ethnic variation can be useful in forensic working hypotheses. Cluster analysis using the highly discriminatory Y-STR 10-plex haplotypes may provide information about ancestry and haplogroup information. Advisors/Committee Members: D’Amato, Maria Eugenia (advisor), Davison, Sean (advisor).

Subjects/Keywords: Polymerase Chain Reaction (PCR); Electrophoresis; Forensic genetics; Population genetics; Khoisan; Botswana

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Tau, T. (2016). A forensic analysis of genetic variation in the Botswana population . (Thesis). University of the Western Cape. Retrieved from http://hdl.handle.net/11394/5657

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Tau, Tiroyamodimo. “A forensic analysis of genetic variation in the Botswana population .” 2016. Thesis, University of the Western Cape. Accessed February 19, 2020. http://hdl.handle.net/11394/5657.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Tau, Tiroyamodimo. “A forensic analysis of genetic variation in the Botswana population .” 2016. Web. 19 Feb 2020.

Vancouver:

Tau T. A forensic analysis of genetic variation in the Botswana population . [Internet] [Thesis]. University of the Western Cape; 2016. [cited 2020 Feb 19]. Available from: http://hdl.handle.net/11394/5657.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Tau T. A forensic analysis of genetic variation in the Botswana population . [Thesis]. University of the Western Cape; 2016. Available from: http://hdl.handle.net/11394/5657

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Debrecen

16. Árgyelán, Tamás. A Phoma lingam szerepének vizsgálata repce levélfoltosság és szárrák előidézésében .

Degree: DE – ATC – Mezőgazdaságtudományi Kar, 2010, University of Debrecen

URL: http://hdl.handle.net/2437/96694

► A repce intenzív termesztéstechnológiájának, a vetésterületének növekedése és a hazai ellenálló fajták hiánya számos meglévő gombás betegség előretörését és új betegségek megjelenését teszi lehetővé. Ezek… (more)

Subjects/Keywords: Phoma lingam; Brassica napus; mesterséges inokulálás; táptalajok; Polymerase Chain Reaction

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APA (6^th Edition):

Árgyelán, T. (2010). A Phoma lingam szerepének vizsgálata repce levélfoltosság és szárrák előidézésében . (Thesis). University of Debrecen. Retrieved from http://hdl.handle.net/2437/96694

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Árgyelán, Tamás. “A Phoma lingam szerepének vizsgálata repce levélfoltosság és szárrák előidézésében .” 2010. Thesis, University of Debrecen. Accessed February 19, 2020. http://hdl.handle.net/2437/96694.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Árgyelán, Tamás. “A Phoma lingam szerepének vizsgálata repce levélfoltosság és szárrák előidézésében .” 2010. Web. 19 Feb 2020.

Vancouver:

Árgyelán T. A Phoma lingam szerepének vizsgálata repce levélfoltosság és szárrák előidézésében . [Internet] [Thesis]. University of Debrecen; 2010. [cited 2020 Feb 19]. Available from: http://hdl.handle.net/2437/96694.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Árgyelán T. A Phoma lingam szerepének vizsgálata repce levélfoltosság és szárrák előidézésében . [Thesis]. University of Debrecen; 2010. Available from: http://hdl.handle.net/2437/96694

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Eastern Michigan University

17. Rolfe, Ashley K. Diet of three mormoopid bats (mormoops blainvillei, pteronotus quadridens, and pteronotus portoricensis ) on Puerto Rico.

Degree: MS, Biology, 2011, Eastern Michigan University

URL: https://commons.emich.edu/theses/349

► This study used visual analysis to determine the percent volume and percent frequency of orders of insects in the guano of the Antillean ghost-faced… (more)

Subjects/Keywords: bats; diet; mormoopidae; pest species; Puerto Rico; polymerase chain reaction; Biology

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APA (6^th Edition):

Rolfe, A. K. (2011). Diet of three mormoopid bats (mormoops blainvillei, pteronotus quadridens, and pteronotus portoricensis ) on Puerto Rico. (Masters Thesis). Eastern Michigan University. Retrieved from https://commons.emich.edu/theses/349

Chicago Manual of Style (16^th Edition):

Rolfe, Ashley K. “Diet of three mormoopid bats (mormoops blainvillei, pteronotus quadridens, and pteronotus portoricensis ) on Puerto Rico.” 2011. Masters Thesis, Eastern Michigan University. Accessed February 19, 2020. https://commons.emich.edu/theses/349.

MLA Handbook (7^th Edition):

Rolfe, Ashley K. “Diet of three mormoopid bats (mormoops blainvillei, pteronotus quadridens, and pteronotus portoricensis ) on Puerto Rico.” 2011. Web. 19 Feb 2020.

Vancouver:

Rolfe AK. Diet of three mormoopid bats (mormoops blainvillei, pteronotus quadridens, and pteronotus portoricensis ) on Puerto Rico. [Internet] [Masters thesis]. Eastern Michigan University; 2011. [cited 2020 Feb 19]. Available from: https://commons.emich.edu/theses/349.

Council of Science Editors:

Rolfe AK. Diet of three mormoopid bats (mormoops blainvillei, pteronotus quadridens, and pteronotus portoricensis ) on Puerto Rico. [Masters Thesis]. Eastern Michigan University; 2011. Available from: https://commons.emich.edu/theses/349

University of Western Ontario

18. Guha Roy, Debanjan. A new algorithm for primer design.

Degree: 2019, University of Western Ontario

URL: https://ir.lib.uwo.ca/etd/6643

► The Polymerase Chain Reaction (PCR) technology is widely used to create DNA copies. It has impacted many diverse fields including genetics, forensics, molecular paleontology, medical… (more)

Subjects/Keywords: DNA; Polymerase Chain Reaction; Primer3; bestPrimer; Other Computer Sciences

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APA (6^th Edition):

Guha Roy, D. (2019). A new algorithm for primer design. (Thesis). University of Western Ontario. Retrieved from https://ir.lib.uwo.ca/etd/6643

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Guha Roy, Debanjan. “A new algorithm for primer design.” 2019. Thesis, University of Western Ontario. Accessed February 19, 2020. https://ir.lib.uwo.ca/etd/6643.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Guha Roy, Debanjan. “A new algorithm for primer design.” 2019. Web. 19 Feb 2020.

Vancouver:

Guha Roy D. A new algorithm for primer design. [Internet] [Thesis]. University of Western Ontario; 2019. [cited 2020 Feb 19]. Available from: https://ir.lib.uwo.ca/etd/6643.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Guha Roy D. A new algorithm for primer design. [Thesis]. University of Western Ontario; 2019. Available from: https://ir.lib.uwo.ca/etd/6643

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Utah

19. Ragsdale, Victoria Elizabeth. Three-dimensional heat transfer analysis of a disposable, continuous-flow polymerase chain reaction device.

Degree: MS, Mechanical Engineering, 2012, University of Utah

URL: http://content.lib.utah.edu/cdm/singleitem/collection/etd3/id/640/rec/2741

► Polymerase Chain Reaction (PCR) is a process which amplifies a specific segment of DNA through a thermal cycling protocol. PCR is largely used in diagnostics,but… (more)

▼ Polymerase Chain Reaction (PCR) is a process which amplifies a specific segment of DNA through a thermal cycling protocol. PCR is largely used in diagnostics,but also has applications in the defense industry and many others. The PCR industry is shifting its focus towards micro-scale devices, and away from macro-scale systems due to i) the micro-scale sample size requiring less blood from patients, ii) total reaction times on the order of minutes opposed to hours and iii) the cost advantages as many microfluidic devices are manufactured from inexpensive polymers. In general, polymers have many advantages including minimal microfabrication processes during manufacturing, wide availability and low cost.This work presents the manufacturing process as well as simulations and testing results of a disposable polycarbonate (PC) device capable of achieving PCR in less than 7 minutes by thermally cycling the sample through an established temperature gradient in aserpentine channel. This device also features a unique laser manufacturing process eliminating the need for a microfabrication facility. Two-dimensional (2D) and three-dimensional (3D) simulations were created to analyze the heat transfer and fluid dynamics throughout the device. Simulations were created both in the flow plane, to analyze the reaction temperatures occurring in each bend of the serpentine channel, and the out of flow plane, to observe the amount of heat leaving the system. Although the 2D simulations did not produce the same values as the 3D models, the same trends wereexhibited so qualitative observations could still be made. Experiments were performed to validate the simulations by comparing the measured device surface temperature from an IR camera to those values from the 3D simulations. It was found that the device producesfluid temperature results within the reaction limits for several flow rates with specific heater temperature settings. An end point PCR melting analysis was also completed on reactants to determine if PCR was achieved. These results were unsuccessful, due tohighly inconsistent flow rates observed during the experiments. Although PCR was not achieved, with slight changes to increase flow rate accuracy, PCR is expected to be successful since the IR images suggest that the fluid is reaching the reaction temperatures.

Subjects/Keywords: Heat transfer; PCR; Continuous-flow; Polymerase chain reaction device

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APA (6^th Edition):

Ragsdale, V. E. (2012). Three-dimensional heat transfer analysis of a disposable, continuous-flow polymerase chain reaction device. (Masters Thesis). University of Utah. Retrieved from http://content.lib.utah.edu/cdm/singleitem/collection/etd3/id/640/rec/2741

Chicago Manual of Style (16^th Edition):

Ragsdale, Victoria Elizabeth. “Three-dimensional heat transfer analysis of a disposable, continuous-flow polymerase chain reaction device.” 2012. Masters Thesis, University of Utah. Accessed February 19, 2020. http://content.lib.utah.edu/cdm/singleitem/collection/etd3/id/640/rec/2741.

MLA Handbook (7^th Edition):

Ragsdale, Victoria Elizabeth. “Three-dimensional heat transfer analysis of a disposable, continuous-flow polymerase chain reaction device.” 2012. Web. 19 Feb 2020.

Vancouver:

Ragsdale VE. Three-dimensional heat transfer analysis of a disposable, continuous-flow polymerase chain reaction device. [Internet] [Masters thesis]. University of Utah; 2012. [cited 2020 Feb 19]. Available from: http://content.lib.utah.edu/cdm/singleitem/collection/etd3/id/640/rec/2741.

Council of Science Editors:

Ragsdale VE. Three-dimensional heat transfer analysis of a disposable, continuous-flow polymerase chain reaction device. [Masters Thesis]. University of Utah; 2012. Available from: http://content.lib.utah.edu/cdm/singleitem/collection/etd3/id/640/rec/2741

University of Utah

20. Hsieh, Fen-Fen. Development of a sensitive polymerase chain reaction assay for group A streptococci;.

Degree: MS;, Pathology;, 1995, University of Utah

URL: http://content.lib.utah.edu/cdm/singleitem/collection/etd1/id/1062/rec/308

► S. pyogenes causes many important human diseases. Rheumatic fever is a systemic inflammatory and nonsuppurative sequla of acute streptococcal pharyngitis that occurs in a small… (more)

Subjects/Keywords: Polymerase Chain Reaction; DNA

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APA (6^th Edition):

Hsieh, F. (1995). Development of a sensitive polymerase chain reaction assay for group A streptococci;. (Masters Thesis). University of Utah. Retrieved from http://content.lib.utah.edu/cdm/singleitem/collection/etd1/id/1062/rec/308

Chicago Manual of Style (16^th Edition):

Hsieh, Fen-Fen. “Development of a sensitive polymerase chain reaction assay for group A streptococci;.” 1995. Masters Thesis, University of Utah. Accessed February 19, 2020. http://content.lib.utah.edu/cdm/singleitem/collection/etd1/id/1062/rec/308.

MLA Handbook (7^th Edition):

Hsieh, Fen-Fen. “Development of a sensitive polymerase chain reaction assay for group A streptococci;.” 1995. Web. 19 Feb 2020.

Vancouver:

Hsieh F. Development of a sensitive polymerase chain reaction assay for group A streptococci;. [Internet] [Masters thesis]. University of Utah; 1995. [cited 2020 Feb 19]. Available from: http://content.lib.utah.edu/cdm/singleitem/collection/etd1/id/1062/rec/308.

Council of Science Editors:

Hsieh F. Development of a sensitive polymerase chain reaction assay for group A streptococci;. [Masters Thesis]. University of Utah; 1995. Available from: http://content.lib.utah.edu/cdm/singleitem/collection/etd1/id/1062/rec/308

21. Kolari, Kai. Fabrication of Silicon and Glass Devices for Microfluidic Bioanalytical Applications.

Degree: 2008, VTT Technical Research Centre of Finland

URL: http://lib.tkk.fi/Diss/2008/isbn9789513870720/

►

This thesis introduces important improvements in fabrication of microfluidic devices on silicon and glass. With the main aim in surface and volume manipulation of aqueous… (more)

▼

This thesis introduces important improvements in fabrication of microfluidic devices on silicon and glass. With the main aim in surface and volume manipulation of aqueous solutions for subsequent biochemical analysis, the backbone of the work has been the development of plasma etching processes for silicon and glass. As the silicon microfabrication technologies are combined with deep anisotropic etching of glass, the processability of microfluidic applications with surface and volume manipulation of fluid is diversified. Several mask materials have been studied with respect to deep plasma etching of glass. As the demand for depth of microfluidic devices extends past 150 µm, the number of usable masking schemes becomes limited. To reach an etch depth beyond 350 µm with aspect ratio of over 3:1 including the mask, silicon shadow mask was used. The results of process development on Al2O3, AlN and TiO2 masks show that a very high etching selectivity on glass can be achieved with these mask materials. The described masking technologies enable e.g. high density of through-a-wafer holes or nearly vertical structuring of glass with great depth. Also, a silicon shadow mask was used for local tuning of hydrophobicity of C4F8 polymer on silicon and glass surfaces by pattering the polymer with O2 plasma through the shadow mask. For both purposes, one silicon shadow mask wafer can be re-used to enable lower processing costs. Thermal manipulation of fluid allows polymerase chain reaction on silicon and glass microchips, but also triggering of capillary action. However, the results of a novel method indicate possible lack of biocompatibility of oxidized silicon surfaces, which may limit the usable microchip surface materials. Microfluidic components with hydrophilic patterning for controlled capillary action can be combined with microphotonics through excitation of fluorescence with evanescent field, which has been characterized with a grating-coupled laser beam.

Työssä käsitellään mikrofluidististen rakenteiden valmistamiseen liittyviä parannuksia. Työn tavoitteena on ollut nesteiden kaksi- ja kolmiulotteinen manipulointi, jonka ytimessä on plasmasyövytysprosessien kehitys piille ja lasille. Piin mikrovalmistusteknologioiden yhdistäminen lasin suurien syvyyksien anisotrooppiseen plasmasyövytykseen luo edellytyksiä uudentyyppisten mikrofluidististen sovellusten valmistamiselle. Lasin plasmasyövytykseen on sovellettu useita eri maskimateriaaleja. Pyrittäessä yli 150 µm:n plasmasyövytyssyvyyteen lasilla maskivaihtoehtojen lukumäärä on vähäinen. Pyrittäessä yli 350 µm syvyyteen ja yli 3:1 aspektisuhteeseen työssä on käytetty piivarjomaskia. Erityisesti alumiinioksidi- ja -alumiininitridimaskeilla voidaan saavuttaa hyvin suuri syöpymisnopeussuhde lasiin. Työssä kuvattuja maskiteknologioita voidaan käyttää mm. läpivientien sekä lähes pystysuorien ja erittäin syvien lasirakenteiden syövyttämiseen. Piivarjomaskiteknologia soveltuu myös käytettäväksi paikallisten hydrofiilisten kuviointien valmistamiseen hydrofobiselle…

Advisors/Committee Members: Helsinki University of Technology, Faculty of Electronics, Communications and Automation.

Subjects/Keywords: glass; plasma etching; hydrophobic coating; shadow mask; polymerase chain reaction

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APA (6^th Edition):

Kolari, K. (2008). Fabrication of Silicon and Glass Devices for Microfluidic Bioanalytical Applications. (Thesis). VTT Technical Research Centre of Finland. Retrieved from http://lib.tkk.fi/Diss/2008/isbn9789513870720/

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Kolari, Kai. “Fabrication of Silicon and Glass Devices for Microfluidic Bioanalytical Applications.” 2008. Thesis, VTT Technical Research Centre of Finland. Accessed February 19, 2020. http://lib.tkk.fi/Diss/2008/isbn9789513870720/.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Kolari, Kai. “Fabrication of Silicon and Glass Devices for Microfluidic Bioanalytical Applications.” 2008. Web. 19 Feb 2020.

Vancouver:

Kolari K. Fabrication of Silicon and Glass Devices for Microfluidic Bioanalytical Applications. [Internet] [Thesis]. VTT Technical Research Centre of Finland; 2008. [cited 2020 Feb 19]. Available from: http://lib.tkk.fi/Diss/2008/isbn9789513870720/.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Kolari K. Fabrication of Silicon and Glass Devices for Microfluidic Bioanalytical Applications. [Thesis]. VTT Technical Research Centre of Finland; 2008. Available from: http://lib.tkk.fi/Diss/2008/isbn9789513870720/

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of South Australia

22. Ellis, Geoffrey Richard. Application of the polymerase chain reaction for the rapid diagnosis of leptospirosis.

Degree: MA, 1995, University of South Australia

URL: http://arrow.unisa.edu.au:8081/1959.8/85113 ; http://arrow.unisa.edu.au/vital/access/manager/Repository/unisa:42819

Subjects/Keywords: Leptospirosis; Polymerase chain reaction.

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APA (6^th Edition):

Ellis, G. R. (1995). Application of the polymerase chain reaction for the rapid diagnosis of leptospirosis. (Masters Thesis). University of South Australia. Retrieved from http://arrow.unisa.edu.au:8081/1959.8/85113 ; http://arrow.unisa.edu.au/vital/access/manager/Repository/unisa:42819

Chicago Manual of Style (16^th Edition):

Ellis, Geoffrey Richard. “Application of the polymerase chain reaction for the rapid diagnosis of leptospirosis.” 1995. Masters Thesis, University of South Australia. Accessed February 19, 2020. http://arrow.unisa.edu.au:8081/1959.8/85113 ; http://arrow.unisa.edu.au/vital/access/manager/Repository/unisa:42819.

MLA Handbook (7^th Edition):

Ellis, Geoffrey Richard. “Application of the polymerase chain reaction for the rapid diagnosis of leptospirosis.” 1995. Web. 19 Feb 2020.

Vancouver:

Ellis GR. Application of the polymerase chain reaction for the rapid diagnosis of leptospirosis. [Internet] [Masters thesis]. University of South Australia; 1995. [cited 2020 Feb 19]. Available from: http://arrow.unisa.edu.au:8081/1959.8/85113 ; http://arrow.unisa.edu.au/vital/access/manager/Repository/unisa:42819.

Council of Science Editors:

Ellis GR. Application of the polymerase chain reaction for the rapid diagnosis of leptospirosis. [Masters Thesis]. University of South Australia; 1995. Available from: http://arrow.unisa.edu.au:8081/1959.8/85113 ; http://arrow.unisa.edu.au/vital/access/manager/Repository/unisa:42819

Heriot-Watt University

23. Gronow, Kathryn L. Studies of the role of the iron oxidizing bacterium Thiobacillus ferrooxidans in marine and terrestrial environments : development of a novel detection assay.

Degree: PhD, 2000, Heriot-Watt University

URL: http://hdl.handle.net/10399/1248

Subjects/Keywords: 579; Polymerase chain reaction; Rusticyanin

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APA (6^th Edition):

Gronow, K. L. (2000). Studies of the role of the iron oxidizing bacterium Thiobacillus ferrooxidans in marine and terrestrial environments : development of a novel detection assay. (Doctoral Dissertation). Heriot-Watt University. Retrieved from http://hdl.handle.net/10399/1248

Chicago Manual of Style (16^th Edition):

Gronow, Kathryn L. “Studies of the role of the iron oxidizing bacterium Thiobacillus ferrooxidans in marine and terrestrial environments : development of a novel detection assay.” 2000. Doctoral Dissertation, Heriot-Watt University. Accessed February 19, 2020. http://hdl.handle.net/10399/1248.

MLA Handbook (7^th Edition):

Gronow, Kathryn L. “Studies of the role of the iron oxidizing bacterium Thiobacillus ferrooxidans in marine and terrestrial environments : development of a novel detection assay.” 2000. Web. 19 Feb 2020.

Vancouver:

Gronow KL. Studies of the role of the iron oxidizing bacterium Thiobacillus ferrooxidans in marine and terrestrial environments : development of a novel detection assay. [Internet] [Doctoral dissertation]. Heriot-Watt University; 2000. [cited 2020 Feb 19]. Available from: http://hdl.handle.net/10399/1248.

Council of Science Editors:

Gronow KL. Studies of the role of the iron oxidizing bacterium Thiobacillus ferrooxidans in marine and terrestrial environments : development of a novel detection assay. [Doctoral Dissertation]. Heriot-Watt University; 2000. Available from: http://hdl.handle.net/10399/1248

University of Edinburgh

24. Cox, Andrew Paul. Epidemiological analysis of host populations with widespread sub-patent infections : African trypanosomiasis.

Degree: PhD, 2007, University of Edinburgh

URL: http://hdl.handle.net/1842/1560

► The epidemiological study of pathogens largely depends on three technologies, serology, microscopy and the polymerase chain reaction (PCR). Serological methods are unable to differentiate between… (more)

▼ The epidemiological study of pathogens largely depends on three technologies, serology, microscopy and the polymerase chain reaction (PCR). Serological methods are unable to differentiate between current and past infections. Microscopy has historically been the mainstay of epidemiological study. In recent times the use of microscopy has been in decline, as it has been shown to have an inherent lack of sensitivity and specificity and produces many false negative results. PCR is now the method of choice for screening samples for the presence or absence of pathogens. Although PCR is widely regarded as an extremely sensitive technique, the fact that it assays a very small volume of sample is often overlooked. If the target pathogen is not present in the tiny aliquot of sample from an infected host, then a false negative results will occur. In endemic situations were the pathogen is present at low infection intensities, then the potential for false negatives results of this type is high. This intensity related false negative effect can lead to serious underestimation of diagnosed prevalence and incidence with consequent misinterpretation of the resulting data. This phenomenon has been reported in the literature for a range of pathogens and especially for epidemiological study of schistosomiasis. The extensive occurrence of false negatives during study of schistosomiasis samples was such an obstacle to epidemiological study it prompted the world health organisation to repeatedly call for quantitative methods to be employed to combat the problem. The main objectives of this thesis are to rationalise and simplify the methods of diagnosing African trypanosomes in epidemiological studies and to investigate the consequences of, and methods of dealing with infection intensity related false negative results that occur as a result of widespread sub-patent infections in the study population A new PCR assay was developed that was capable of analysing whole blood placed onto treated filter paper. The PCR assay was capable of differentiating between all the important African trypanosome species, producing a unique size of amplicon for each species of trypanosome. Initial results from repeated screening of human and cattle samples known to be parasitologically positive indicated that many false negative results occur. A more extensive analysis of thirty five bovine blood samples randomly chosen from a collection of field samples revealed that false negative results occurred regularly. The prevalence of infection after a single screening was 14.3% whereas the cumulative prevalence after over 100 repeated screenings rose to 85.7%. This showed that a severe underestimation of prevalence occurs from a single screening of the samples. In order to investigate the consequences of, and develop methods of dealing with this problem, computer based simulations were used to model the dynamics of screening samples with sub-patent infections. In order to construct the model the data obtained from repeat screening of the thirty-five bovine blood…

Subjects/Keywords: 614.4; epidemiology; polymerase chain reaction; serology; African trypanosome

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APA (6^th Edition):

Cox, A. P. (2007). Epidemiological analysis of host populations with widespread sub-patent infections : African trypanosomiasis. (Doctoral Dissertation). University of Edinburgh. Retrieved from http://hdl.handle.net/1842/1560

Chicago Manual of Style (16^th Edition):

Cox, Andrew Paul. “Epidemiological analysis of host populations with widespread sub-patent infections : African trypanosomiasis.” 2007. Doctoral Dissertation, University of Edinburgh. Accessed February 19, 2020. http://hdl.handle.net/1842/1560.

MLA Handbook (7^th Edition):

Cox, Andrew Paul. “Epidemiological analysis of host populations with widespread sub-patent infections : African trypanosomiasis.” 2007. Web. 19 Feb 2020.

Vancouver:

Cox AP. Epidemiological analysis of host populations with widespread sub-patent infections : African trypanosomiasis. [Internet] [Doctoral dissertation]. University of Edinburgh; 2007. [cited 2020 Feb 19]. Available from: http://hdl.handle.net/1842/1560.

Council of Science Editors:

Cox AP. Epidemiological analysis of host populations with widespread sub-patent infections : African trypanosomiasis. [Doctoral Dissertation]. University of Edinburgh; 2007. Available from: http://hdl.handle.net/1842/1560

University of Pretoria

25. Bagla, Victor Patrick. The demonstration of lumpy skin disease virus in semen of experimentally infected bulls using different diagnostic techniques.

Degree: Veterinary Tropical Diseases, 2008, University of Pretoria

URL: http://hdl.handle.net/2263/25041

► Lumpy skin disease virus (LSDV), a poxvirus that belongs to the genus Capripoxvirus is an important pathogen that can be shed in the semen of… (more)

▼ Lumpy skin disease virus (LSDV), a poxvirus that belongs to the genus Capripoxvirus is an important pathogen that can be shed in the semen of infected bulls. The screening of semen for infectious virus prior to artificial insemination requires a sensitive diagnostic method. The isolation of the virus on cell cultures and/or the use of polymerase chain reaction (PCR) are sensitive diagnostic tests which can be used to screen semen for LSD viral DNA prior to artificial insemination. Although cell culture is a sensitive method and detects infectious virus, its use has major limitations due to the toxic effect of semen on the cells. This study was therefore aimed at finding a method that decreases the toxic effect of semen on cell culture and enhances LSDV isolation. Secondly, the efficiency of this method in enhancing the isolation of LSDV in field samples was tested. In order to eliminate the toxic effect of semen on cell culture, a pilot study was conducted in which semen samples from LSDV sero-negative bulls were collected and infected with a field isolate of LSDV, strain 248/93 with a titre of 6.5 log TCID50. The semen samples were subjected to one of four different methods, viz centrifugation, serial dilution, filtration and chemical treatment with kaolin. The centrifugation, serial dilution, and filtration methods were supplemented with additional amounts of gentamycin. The toxic effects of semen on cell culture were completely eliminated when supernatants of semen samples, centrifuged at 2000 rpm for 1, 3 and 5 mins and serial diluted was used to inoculate confluent monolayers of bovine dermis cells. Semen diluted in MEM with or without additional antibiotics was the most sensitive method of demonstrating virus at higher dilutions, followed by pellets of samples centrifuged for 1 and 3 minutes. The toxicity recorded when the pellet fraction of semen samples were centrifuged for 5 mins at 2000 rpm was comparable to results obtained from serially diluted samples supplemented with gentamycin. The use of filtration and kaolin treatment of semen samples could not remove the toxic effect of semen on cells. To evaluate the presence of LSDV in semen of experimentally infected bulls, six seronegative post-pubertal bulls housed in an insect proof facility were infected with LSDV via the intravenous route. The experimentally infected bulls were monitored for clinical sign of the disease. Two bulls showed severe, two a mild and two an inapparent infection. Blood samples were collected for virus isolation and semen samples for virus isolation and PCR. Vesicular fluid and preputial washes were also investigated for the presence of LSD viral nucleic acid using PCR. The infectious titre of the virus shed in semen of these bulls was also calculated. The incubation period in infected bulls varied from 7 to 14 days. The length of viraemia varied between groups and did not correlate with the severity of clinical disease. The virus was isolated from blood samples of bulls in the severely infected group on several occasions. Bulls in… Advisors/Committee Members: Prof E H Venter (advisor).

Subjects/Keywords: Lumpy skin disease virus; Viral nucleic acid; Polymerase chain reaction; UCTD

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APA (6^th Edition):

Bagla, V. P. (2008). The demonstration of lumpy skin disease virus in semen of experimentally infected bulls using different diagnostic techniques. (Masters Thesis). University of Pretoria. Retrieved from http://hdl.handle.net/2263/25041

Chicago Manual of Style (16^th Edition):

Bagla, Victor Patrick. “The demonstration of lumpy skin disease virus in semen of experimentally infected bulls using different diagnostic techniques.” 2008. Masters Thesis, University of Pretoria. Accessed February 19, 2020. http://hdl.handle.net/2263/25041.

MLA Handbook (7^th Edition):

Bagla, Victor Patrick. “The demonstration of lumpy skin disease virus in semen of experimentally infected bulls using different diagnostic techniques.” 2008. Web. 19 Feb 2020.

Vancouver:

Bagla VP. The demonstration of lumpy skin disease virus in semen of experimentally infected bulls using different diagnostic techniques. [Internet] [Masters thesis]. University of Pretoria; 2008. [cited 2020 Feb 19]. Available from: http://hdl.handle.net/2263/25041.

Council of Science Editors:

Bagla VP. The demonstration of lumpy skin disease virus in semen of experimentally infected bulls using different diagnostic techniques. [Masters Thesis]. University of Pretoria; 2008. Available from: http://hdl.handle.net/2263/25041

University of Pretoria

26. [No author]. Development of PCR-based methods for detection of African lyssaviruses .

Degree: 2010, University of Pretoria

URL: http://upetd.up.ac.za/thesis/available/etd-10082010-143715/

► The etiological agent of rabies encephalitis belongs to the genus Lyssavirus in the Rhabdoviridae family. Lyssaviruses are negative sense, single stranded RNA viruses and cause… (more)

▼ The etiological agent of rabies encephalitis belongs to the genus Lyssavirus in the Rhabdoviridae family. Lyssaviruses are negative sense, single stranded RNA viruses and cause an estimated 55 000 human deaths per year with 44% of these deaths occurring in Africa (WHO, 2005). With intense research effort and increased sequence information it is becoming evident that the Lyssavirus genus is much more diverse than initially thought and therefore diagnostic methods need to be modified accordingly. The African continent sustains a diverse variety of lyssaviruses, however, most countries in Africa do not have active surveillance or necessary diagnostic tools and therefore rabies-related lyssaviruses are underreported. Previous studies have indicated that real-time PCR has improved sensitivity and rapidity over conventional molecular diagnostic methods with the added advantage of allowing accurate estimations of viral load in a wide variety of samples. Several realtime PCR assays have been developed; however, none were specifically aimed at detection of lyssaviruses present on the African continent. This study was therefore aimed at evaluating certain molecular diagnostic methods for the detection of African lyssaviruses. Furthermore, the application of real-time PCR for various fields in lyssavirus research i.e. diagnostics, surveillance and pathogenicity studies were evaluated. This study revealed two different hemi-nested PCR assays capable of detecting representatives of African lyssaviruses. A real-time PCR was developed that was successful for the detection of African lyssaviruses. In addition, a quantitative assay and internal control was successfully employed for confirming ante-mortem human rabies diagnosis as well as post-mortem animal rabies diagnosis in formalin fixed brain material. As such the real-time PCR assay developed in this study could therefore be routinely used for ante-mortem diagnosis and as a confirmatory test for post-mortem diagnosis. The ability of this assay to detect and quantify all currently known African lyssaviruses not only offers improved surveillance capacity, but offers unique potential as a sensitive tool to track virus movement in pathogenicity studies. These aspects are important in our search for a better understanding of the complex epidemiological and viral characteristics of African lyssaviruses. Copyright Advisors/Committee Members: Weyer, Jacqueline (advisor), Nel, Louis Hendrik (advisor), Markotter, Wanda (advisor).

Subjects/Keywords: African lyssaviruses; Polymerase chain reaction (PCR); Pcr-based methods; UCTD

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

author], [. (2010). Development of PCR-based methods for detection of African lyssaviruses . (Masters Thesis). University of Pretoria. Retrieved from http://upetd.up.ac.za/thesis/available/etd-10082010-143715/

Chicago Manual of Style (16^th Edition):

author], [No. “Development of PCR-based methods for detection of African lyssaviruses .” 2010. Masters Thesis, University of Pretoria. Accessed February 19, 2020. http://upetd.up.ac.za/thesis/available/etd-10082010-143715/.

MLA Handbook (7^th Edition):

author], [No. “Development of PCR-based methods for detection of African lyssaviruses .” 2010. Web. 19 Feb 2020.

Vancouver:

author] [. Development of PCR-based methods for detection of African lyssaviruses . [Internet] [Masters thesis]. University of Pretoria; 2010. [cited 2020 Feb 19]. Available from: http://upetd.up.ac.za/thesis/available/etd-10082010-143715/.

Council of Science Editors:

author] [. Development of PCR-based methods for detection of African lyssaviruses . [Masters Thesis]. University of Pretoria; 2010. Available from: http://upetd.up.ac.za/thesis/available/etd-10082010-143715/

Universidade Federal de Mato Grosso do Sul

27. Cominetti, Marlon Cezar. Ecoepidemiologia da Entomofauna Triatominae (Hemiptera: Reduviidae) e distribuição populacional das discretas unidades de tipagem (DTU) de Trypanosoma cruzi em Mato Grosso do Sul, Brasil .

Degree: 2015, Universidade Federal de Mato Grosso do Sul

URL: http://repositorio.cbc.ufms.br:8080/jspui/handle/123456789/2914

► Este trabalho decorre do estudo ecoepidemiologico da entomofauna Triatominae (Hemiptera: Reduviidae) e distribuição populacional das unidades discretas de tipagem (DTU) de Trypanosoma cruzi em Mato… (more)

▼ Este trabalho decorre do estudo ecoepidemiologico da entomofauna Triatominae (Hemiptera: Reduviidae) e distribuição populacional das unidades discretas de tipagem (DTU) de Trypanosoma cruzi em Mato Grosso do Sul, Brasil. Foi analisado o conteúdo intestinal de 515 triatomíneos, utilizando-se microscopia óptica e a Reação em Cadeia da Polimerase (PCR) para identificação de Trypanosoma cruzi. As amostras positivas para o parasito, proveniente de 12 triatomíneos, foram submetidas a uma segunda PCR, cujo alvo era o gene cromossômico TcSC5D, seguido da técnica de polimorfismo no comprimento de fragmentos de restrição (RFLP) com enzimas de restrição HpaI e SphI, clonagem e sequenciamento do amplificado. Em uma segunda etapa foram analisados, a partir de dados secundários, 14.178 espécimes de insetos vetores de T. cruzi capturados em 50 municípios de Mato Grosso do Sul em um período de nove anos e a influência de fatores climáticos, como temperatura, umidade relativa do ar e precipitação, sobre sua frequência. Observou-se que a PCR foi mais precisa na identificação do parasito que o exame de microscopia (19,6% e 11,3%, respectivamente). Foram identificadas três unidades discretas de tipagem (DTU): TcI (58,34%), TcII (33,33%) e TcBat/TcVII (8,33%). A espécie mais frequente no estado é Triatoma sordida (96,22%) e a maioria dos espécimes foi coletada em peridomicílio (85,06%). Verificou-se que a frequência desses insetos foi afetada por fatores climáticos como temperatura e umidade relativa do ar, sendo esta última a mais influente. Este trabalho aponta que os dados oficiais das taxas de infecção natural dos triatomíneos podem estar subestimados em todo o estado, apresenta a distribuição de três linhagens de T. cruzi, sendo que esse é o primeiro relato de infecção natural por TcBat/TcVII em triatomíneos e que as características climáticas ao longo do ano devem ser observadas pelas equipes de controle de vetores. Tais achados podem subsidiar programas de controle do vetor, propiciando a otimização dos recursos destinados às ações de prevenção e controle da transmissão vetorial de T. cruzi em Mato Grosso do Sul. Advisors/Committee Members: Andreotti, Renato (advisor).

Subjects/Keywords: Trypanosoma cruzi; Triatominae; Reação em Cadeia da Polimerase; Polymerase Chain Reaction

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Cominetti, M. C. (2015). Ecoepidemiologia da Entomofauna Triatominae (Hemiptera: Reduviidae) e distribuição populacional das discretas unidades de tipagem (DTU) de Trypanosoma cruzi em Mato Grosso do Sul, Brasil . (Doctoral Dissertation). Universidade Federal de Mato Grosso do Sul. Retrieved from http://repositorio.cbc.ufms.br:8080/jspui/handle/123456789/2914

Chicago Manual of Style (16^th Edition):

Cominetti, Marlon Cezar. “Ecoepidemiologia da Entomofauna Triatominae (Hemiptera: Reduviidae) e distribuição populacional das discretas unidades de tipagem (DTU) de Trypanosoma cruzi em Mato Grosso do Sul, Brasil .” 2015. Doctoral Dissertation, Universidade Federal de Mato Grosso do Sul. Accessed February 19, 2020. http://repositorio.cbc.ufms.br:8080/jspui/handle/123456789/2914.

MLA Handbook (7^th Edition):

Cominetti, Marlon Cezar. “Ecoepidemiologia da Entomofauna Triatominae (Hemiptera: Reduviidae) e distribuição populacional das discretas unidades de tipagem (DTU) de Trypanosoma cruzi em Mato Grosso do Sul, Brasil .” 2015. Web. 19 Feb 2020.

Vancouver:

Cominetti MC. Ecoepidemiologia da Entomofauna Triatominae (Hemiptera: Reduviidae) e distribuição populacional das discretas unidades de tipagem (DTU) de Trypanosoma cruzi em Mato Grosso do Sul, Brasil . [Internet] [Doctoral dissertation]. Universidade Federal de Mato Grosso do Sul; 2015. [cited 2020 Feb 19]. Available from: http://repositorio.cbc.ufms.br:8080/jspui/handle/123456789/2914.

Council of Science Editors:

Cominetti MC. Ecoepidemiologia da Entomofauna Triatominae (Hemiptera: Reduviidae) e distribuição populacional das discretas unidades de tipagem (DTU) de Trypanosoma cruzi em Mato Grosso do Sul, Brasil . [Doctoral Dissertation]. Universidade Federal de Mato Grosso do Sul; 2015. Available from: http://repositorio.cbc.ufms.br:8080/jspui/handle/123456789/2914

28. Λάζαρη, Σπυριδούλα. Polymerase chain reaction as a succesful biotechnological application. Ways we use PCR in the fields of bioinformatics forensics and genetics.

Degree: 2011, University of Patras

URL: http://nemertes.lis.upatras.gr/jspui/handle/10889/4575

►

Molecular genetics use molecular methods that amplify specific fragments of DNA. Today, the molecular techniques which were developed for amplification and detection of specific sequences… (more)

▼

Molecular genetics use molecular methods that amplify specific fragments of DNA. Today, the molecular techniques which were developed for amplification and detection of specific sequences of nucleic acids helped in a great deal to understand the structure of many diseases. Polymerase chain reaction or PCR is a technique that is used for isolation and amplification of a specific sequence of DNA. PCR is an in vitro method that exploits the in vivo procedure of replication of DNA. DNA polymerase is used to amplify a piece of DNA by in vitro enzymatic replication. As PCR progresses, the DNA thus generated is itself used as a template for replication. This sets in motion a chain reaction in which the DNA template is exponentially amplified. With PCR it is possible to amplify a single or few copies of a piece of DNA across several orders of magnitude, generating millions or more copies of the DNA piece. PCR was designed and presented by Dr Kary Mullis (1983).Today PCR has a great range of implementations related to 1) the cloning and the study of gene’s expression 2) the detection of mutations that are responsible for hereditary diseases 3) Criminology, Toxicology and Forensics.

H Μοριακή Γενετική χρησιμοποιεί μοριακές μεθόδους οι οποίες ενισχύουν συγκεκριμένα τμήματα DNA. Σήμερα, οι μοριακές τεχνικές οι οποίες αναπτύχθηκαν για ενίσχυση και ανίχνευση συγκεκριμένων ακολουθιών νουκλεϊνικών οξέων βοήθησαν σε μεγάλο βαθμό στην κατανόηση της δομής πολλών ασθενειών. Η αλυσιδωτή αντίδραση πολυμεράσης είναι μια τεχνική η οποία χρησιμοποιείται για απομόνωση και ενίσχυση μιας συγκεκριμένης ακολουθίας DNA. Η αλυσιδωτή αντίδραση της πολυμεράσης είναι μια διαδικασία που πραγματοποιείται σε ελεγχόμενες συνθήκες έξω απο ζωντανούς οργανισμούς, η οποία εκμεταλλεύεται την αντιγραφή του DNA που πραγματοποιείται μεσα στον ζωντανό οργανισμό. Όσο η PCR εξελίσσεται δημιουργούνται αντίγραφα DNA χρησιμοποιώντας ως πρότυπο το αρχικό DNA. Αυτό ενεργοποιεί μια διαδικασία αλυσιδωτής αντίδρασης ώπου το DNA αναπαράγεται εκθετικά. Με την PCR μπορείς να δημιουργήσεις εκατομμύρια αντίγραφα DNA από μία συγκεκριμένη ακολουθία. Η PCR σχεδιάστηκε και παρουσιάστηκε απο τον Dr Kary Mullis. Σήμερα η PCR έχει μέγαλο εύρος εφαρμογής σε πεδία σχετικά με τη μελέτη της γενετικής έκφρασης την ανίχνευση μεταλλάξεων οι οποίες είναι υπεύθυνες για κληρονομικές ασθένειες, Εγκληματολογία, Τοξικολογία και Ιατροδικαστική.

Advisors/Committee Members: Ματσόπουλος, Γεώργιος, Lazari, Spyridoula, Ματσόπουλος, Γεώργιος, Ουζούνογλου, Νικόλαος, Κουτσούρης, Διονύσιος.

Subjects/Keywords: Polymerase chain reaction (PCR); Genetics; 572.43; Αλυσιδωτή αντίδραση πολυμεράσης; Γενετική

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Λάζαρη, �. (2011). Polymerase chain reaction as a succesful biotechnological application. Ways we use PCR in the fields of bioinformatics forensics and genetics. (Masters Thesis). University of Patras. Retrieved from http://nemertes.lis.upatras.gr/jspui/handle/10889/4575

Chicago Manual of Style (16^th Edition):

Λάζαρη, Σπυριδούλα. “Polymerase chain reaction as a succesful biotechnological application. Ways we use PCR in the fields of bioinformatics forensics and genetics.” 2011. Masters Thesis, University of Patras. Accessed February 19, 2020. http://nemertes.lis.upatras.gr/jspui/handle/10889/4575.

MLA Handbook (7^th Edition):

Λάζαρη, Σπυριδούλα. “Polymerase chain reaction as a succesful biotechnological application. Ways we use PCR in the fields of bioinformatics forensics and genetics.” 2011. Web. 19 Feb 2020.

Vancouver:

Λάζαρη �. Polymerase chain reaction as a succesful biotechnological application. Ways we use PCR in the fields of bioinformatics forensics and genetics. [Internet] [Masters thesis]. University of Patras; 2011. [cited 2020 Feb 19]. Available from: http://nemertes.lis.upatras.gr/jspui/handle/10889/4575.

Council of Science Editors:

Λάζαρη �. Polymerase chain reaction as a succesful biotechnological application. Ways we use PCR in the fields of bioinformatics forensics and genetics. [Masters Thesis]. University of Patras; 2011. Available from: http://nemertes.lis.upatras.gr/jspui/handle/10889/4575

University of Hawaii – Manoa

29. Roll, Bruce M. Development of polymerase chain reaction techniques for the detection of waterborne pathogens in environmental waters.

Degree: PhD, 2009, University of Hawaii – Manoa

URL: http://hdl.handle.net/10125/9994

►

Microfiche.

xiii, 131 leaves, bound ill. 29 cm

Currently water quality indicator bacteria (Escherichia coli, enterococci) are utilized to determine the sanitary quality of recreational… (more)

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Microfiche.

xiii, 131 leaves, bound ill. 29 cm

Currently water quality indicator bacteria (Escherichia coli, enterococci) are utilized to determine the sanitary quality of recreational waters. These indicators are normal inhabitants of the intestinal tract of warm-blooded animals and their presence in recreational water is considered indicative of fecal contamination. However, in tropical climates E. coli and enterococci appear to be naturally present in tropical fresh waters and soil. This has questioned the use of E. coli and enterococci as water quality indicators for tropical climates and demonstrates the need for alternative means to determine the sanitary quality of recreational waters. The development of the polymerase chain reaction (PCR) method has offered an attractive alternative to the problematic cultural isolation of indicator bacteria and pathogens. The study utilize PCR to detect waterborne pathogens and indicator bacteria in environmental waters. In addition survival experiments were conducted to determine the persistence of PCR-target gene sequences in ocean water. A pair of PCR primers were chosen targeting a Salmonella spp. gene sequence. These primers were specific for 25 Salmonella spp. and did not cross react with 27 different non Salmonella bacteria. In seeding experiments the sensitivity of these primers was 12 CFU (Salmonella typhimurium) in primary treated sewage and 1 CFU in ocean water. In addition, field studies were conducted using PCR and standard culture method. A total of six field samples were tested with these primers and 3 demonstrated the presence of Salmonella spp. Two of the PCR positive samples were positive for culturally viable Salmonella spp. A set of PCR primers were developed to detect Bacteroides spp., the most common inhabitant of the human intestinal tract. These primers were specific for 4 Bacteroides spp. and did not cross react with 27 non Bacteroides isolates. These primers were able to detect Bacteroides spp. in a variety of environmental waters. Survival experiments using E. coli and Salmonella typhimurium were conducted in ocean water and demonstrated a loss of E. coli and S. typhimurium gene sequences after exposure for 14 and 16 days, respectively. In summary this research supports the use of PCR as a water quality monitoring tool.

Subjects/Keywords: Polymerase chain reaction; Marine microbiology; Salmonella typhimurium; Waterborne infection

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Roll, B. M. (2009). Development of polymerase chain reaction techniques for the detection of waterborne pathogens in environmental waters. (Doctoral Dissertation). University of Hawaii – Manoa. Retrieved from http://hdl.handle.net/10125/9994

Chicago Manual of Style (16^th Edition):

Roll, Bruce M. “Development of polymerase chain reaction techniques for the detection of waterborne pathogens in environmental waters.” 2009. Doctoral Dissertation, University of Hawaii – Manoa. Accessed February 19, 2020. http://hdl.handle.net/10125/9994.

MLA Handbook (7^th Edition):

Roll, Bruce M. “Development of polymerase chain reaction techniques for the detection of waterborne pathogens in environmental waters.” 2009. Web. 19 Feb 2020.

Vancouver:

Roll BM. Development of polymerase chain reaction techniques for the detection of waterborne pathogens in environmental waters. [Internet] [Doctoral dissertation]. University of Hawaii – Manoa; 2009. [cited 2020 Feb 19]. Available from: http://hdl.handle.net/10125/9994.

Council of Science Editors:

Roll BM. Development of polymerase chain reaction techniques for the detection of waterborne pathogens in environmental waters. [Doctoral Dissertation]. University of Hawaii – Manoa; 2009. Available from: http://hdl.handle.net/10125/9994

University of Hong Kong

30. 顔鴻儀; Ngan, Hung-yee. Molecular diagnosis of penicilliosis marneffei.

Degree: Master of Medical Sciences, 2001, University of Hong Kong

URL: Ngan, H. [顔鴻儀]. (2001). Molecular diagnosis of penicilliosis marneffei. (Thesis). University of Hong Kong, Pokfulam, Hong Kong SAR. Retrieved from http://dx.doi.org/10.5353/th_b3197003 ; http://dx.doi.org/10.5353/th_b3197003 ; http://hdl.handle.net/10722/28847

published_or_final_version

Medical Sciences

Master

Master of Medical Sciences

Subjects/Keywords: Mycoses.; Polymerase chain reaction

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

顔鴻儀; Ngan, H. (2001). Molecular diagnosis of penicilliosis marneffei. (Masters Thesis). University of Hong Kong. Retrieved from Ngan, H. [顔鴻儀]. (2001). Molecular diagnosis of penicilliosis marneffei. (Thesis). University of Hong Kong, Pokfulam, Hong Kong SAR. Retrieved from http://dx.doi.org/10.5353/th_b3197003 ; http://dx.doi.org/10.5353/th_b3197003 ; http://hdl.handle.net/10722/28847

Chicago Manual of Style (16^th Edition):

顔鴻儀; Ngan, Hung-yee. “Molecular diagnosis of penicilliosis marneffei.” 2001. Masters Thesis, University of Hong Kong. Accessed February 19, 2020. Ngan, H. [顔鴻儀]. (2001). Molecular diagnosis of penicilliosis marneffei. (Thesis). University of Hong Kong, Pokfulam, Hong Kong SAR. Retrieved from http://dx.doi.org/10.5353/th_b3197003 ; http://dx.doi.org/10.5353/th_b3197003 ; http://hdl.handle.net/10722/28847.

MLA Handbook (7^th Edition):

顔鴻儀; Ngan, Hung-yee. “Molecular diagnosis of penicilliosis marneffei.” 2001. Web. 19 Feb 2020.

Vancouver:

顔鴻儀; Ngan H. Molecular diagnosis of penicilliosis marneffei. [Internet] [Masters thesis]. University of Hong Kong; 2001. [cited 2020 Feb 19]. Available from: Ngan, H. [顔鴻儀]. (2001). Molecular diagnosis of penicilliosis marneffei. (Thesis). University of Hong Kong, Pokfulam, Hong Kong SAR. Retrieved from http://dx.doi.org/10.5353/th_b3197003 ; http://dx.doi.org/10.5353/th_b3197003 ; http://hdl.handle.net/10722/28847.

Council of Science Editors:

顔鴻儀; Ngan H. Molecular diagnosis of penicilliosis marneffei. [Masters Thesis]. University of Hong Kong; 2001. Available from: Ngan, H. [顔鴻儀]. (2001). Molecular diagnosis of penicilliosis marneffei. (Thesis). University of Hong Kong, Pokfulam, Hong Kong SAR. Retrieved from http://dx.doi.org/10.5353/th_b3197003 ; http://dx.doi.org/10.5353/th_b3197003 ; http://hdl.handle.net/10722/28847

Open Access Theses and Dissertations