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You searched for subject:(Polyclonal formation). Showing records 1 – 2 of 2 total matches.

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Eastern Michigan University

1. Biwa, Valerie. The Sojourner experience: An exploration of identity transformation through communication.

Degree: MA, Communication, Media and Theatre Arts, 2016, Eastern Michigan University

This exploratory research focuses on the relationship between communication and identity transformation in the sojourner experience. It inquires the following: RQ1: How does communication affect the identity transformation process of sojourners? RQ2: How does identity transformation affect the way sojourners communicate? Ten participants from six countries were interviewed in a forty (40) minute interview session. A qualitative methodology with an open-minded exploratory approach using semi-structured questions to guide the interview was employed. A grounded-in-data coding and Owen’s criteria for identifying themes (Owen, 1984) was used to identify emerging themes and overlapping narratives. The study revealed five themes from the “living abroad” experience: changing the way I talk, interacting with the host culture, meeting new people, the college experience, the self; and five themes from the “at-home” experience: I am more, is this home? people at home, at work, and where do I fit in to answer RQ1 and RQ2, respectively. Advisors/Committee Members: Dennis P. O'Grady, Nick Romerhausen, Tsai-Shan Shen.

Subjects/Keywords: DNA Sequence; Genomic sequencing; Next Generation Sequencing; PCR duplications; Personalized Medicine; Polyclonal formation; Arts and Humanities; Theatre and Performance Studies

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APA (6th Edition):

Biwa, V. (2016). The Sojourner experience: An exploration of identity transformation through communication. (Masters Thesis). Eastern Michigan University. Retrieved from https://commons.emich.edu/theses/794

Chicago Manual of Style (16th Edition):

Biwa, Valerie. “The Sojourner experience: An exploration of identity transformation through communication.” 2016. Masters Thesis, Eastern Michigan University. Accessed September 28, 2020. https://commons.emich.edu/theses/794.

MLA Handbook (7th Edition):

Biwa, Valerie. “The Sojourner experience: An exploration of identity transformation through communication.” 2016. Web. 28 Sep 2020.

Vancouver:

Biwa V. The Sojourner experience: An exploration of identity transformation through communication. [Internet] [Masters thesis]. Eastern Michigan University; 2016. [cited 2020 Sep 28]. Available from: https://commons.emich.edu/theses/794.

Council of Science Editors:

Biwa V. The Sojourner experience: An exploration of identity transformation through communication. [Masters Thesis]. Eastern Michigan University; 2016. Available from: https://commons.emich.edu/theses/794


Eastern Michigan University

2. Aldilaimi, Akram. A comparison of two methods of template amplification for next generation sequencing: Implications of polyclonal formation on DNA sequence for several cancer tissues.

Degree: MS, Health Sciences, 2016, Eastern Michigan University

Next-Generation Sequencing (NGS) technology has advanced the field of personalized medicine by predicting effective treatments for cancer patients using genomic sequence data, including the detection of oncogenes (genes involved in cancer development). Simultaneous amplification of multiple DNA templates (referred to as polyclonal formation) is a primary disadvantage when preparing templates for NGS resulting in redundancies in DNA sequences or nonspecific noise in the sequencing. This study was conducted to compare DNA templates for sequencing prepared using two instruments the Thermo Fisher Ion Chef (IC) and Thermo Fisher OneTouch-2 (OT2). Six sequencing metrics obtained from 114 sequencing trials for evaluation: polyclonal reads, total sequencing reads, empty microcell well reads, no-template reads, useable number reads, and library number reads. A comparison of mean sequencing metrics between the IC and OT2 methods established that for four of the six metrics, IC was the preferred operation for DNA template preparation due to the less polyclonal formation. Advisors/Committee Members: Irwin Martin, David Kass, Joseph Paulauskis.

Subjects/Keywords: DNA Sequence; Genomic sequencing; Next Generation Sequencing; PCR duplications; Personalized Medicine; Polyclonal formation; Medicine and Health Sciences

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6th Edition):

Aldilaimi, A. (2016). A comparison of two methods of template amplification for next generation sequencing: Implications of polyclonal formation on DNA sequence for several cancer tissues. (Masters Thesis). Eastern Michigan University. Retrieved from https://commons.emich.edu/theses/790

Chicago Manual of Style (16th Edition):

Aldilaimi, Akram. “A comparison of two methods of template amplification for next generation sequencing: Implications of polyclonal formation on DNA sequence for several cancer tissues.” 2016. Masters Thesis, Eastern Michigan University. Accessed September 28, 2020. https://commons.emich.edu/theses/790.

MLA Handbook (7th Edition):

Aldilaimi, Akram. “A comparison of two methods of template amplification for next generation sequencing: Implications of polyclonal formation on DNA sequence for several cancer tissues.” 2016. Web. 28 Sep 2020.

Vancouver:

Aldilaimi A. A comparison of two methods of template amplification for next generation sequencing: Implications of polyclonal formation on DNA sequence for several cancer tissues. [Internet] [Masters thesis]. Eastern Michigan University; 2016. [cited 2020 Sep 28]. Available from: https://commons.emich.edu/theses/790.

Council of Science Editors:

Aldilaimi A. A comparison of two methods of template amplification for next generation sequencing: Implications of polyclonal formation on DNA sequence for several cancer tissues. [Masters Thesis]. Eastern Michigan University; 2016. Available from: https://commons.emich.edu/theses/790

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