subject:(Polyamine)

NSYSU

1. Chang, Pao-Shu. The Effect of Polyamines on Floral Initiation and Flower Development in Polianthes tuberosa.

Degree: Master, Biological Sciences, 2003, NSYSU

URL: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0211103-162955

► Abstract In the day-neutral plant Polianthes tuberosa L.ï¼cv. Doubleï¼putrescine and spermine in corms at the early floral initiation stage decreased by 26 and 35ï¹ª,respectively, compared… (more)

Subjects/Keywords: flowering; polyamine

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APA (6^th Edition):

Chang, P. (2003). The Effect of Polyamines on Floral Initiation and Flower Development in Polianthes tuberosa. (Thesis). NSYSU. Retrieved from http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0211103-162955

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Chang, Pao-Shu. “The Effect of Polyamines on Floral Initiation and Flower Development in Polianthes tuberosa.” 2003. Thesis, NSYSU. Accessed March 05, 2021. http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0211103-162955.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Chang, Pao-Shu. “The Effect of Polyamines on Floral Initiation and Flower Development in Polianthes tuberosa.” 2003. Web. 05 Mar 2021.

Vancouver:

Chang P. The Effect of Polyamines on Floral Initiation and Flower Development in Polianthes tuberosa. [Internet] [Thesis]. NSYSU; 2003. [cited 2021 Mar 05]. Available from: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0211103-162955.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Chang P. The Effect of Polyamines on Floral Initiation and Flower Development in Polianthes tuberosa. [Thesis]. NSYSU; 2003. Available from: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0211103-162955

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Pretoria

2. Williams, Marni. Delineation of functional roles of parasite-specific inserts in the malarial S-adenosylmethionine decarboxylase / ornithine decarboxylase .

Degree: 2008, University of Pretoria

URL: http://upetd.up.ac.za/thesis/available/etd-08042008-072858/

► The polyamines putrescine, spermidine and spermine play essential roles in the proliferation and differentiation of most eukaryotic cells. Inhibition of the polyamine pathway is known… (more)

▼ The polyamines putrescine, spermidine and spermine play essential roles in the proliferation and differentiation of most eukaryotic cells. Inhibition of the polyamine pathway is known to have antitumour and antiparasitic effects and á-difluoromethylornithine (DFMO), a polyamine biosynthesis inhibitor, is clinically used in the treatment of African sleeping sickness caused by Trypanosoma brucei gambiense. Ornithine decarboxylase (ODC) and Sadenosylmethionine decarboxylase (AdoMetDC) are the rate-limiting enzymes in polyamine metabolism. Usually, these enzymes are individually regulated, however, in the malaria parasite, Plasmodium falciparum, these enzymes are part of a unique bifunctional PfAdoMetDC/ODC protein. In addition, compared to homologous proteins, this malarial protein contains six unique parasite-specific inserted regions, which can be targeted with novel drugs. A modified restriction enzyme-mediated inverse PCR method was developed to delete the largest parasite-specific insert (411 bp) from the large PfAdoMetDC/ODC gene (4257 bp). The method was compared to existing deletion mutagenesis PCR protocols and was shown to be the most effective method (80% mutagenesis efficiency) as opposed to the 40% positively mutated clones obtained with the overlapping primer method in deleting a >100 bp region. The independent removal of all three the PfAdoMetDC domain parasite-specific inserts and subsequent activity analysis thereof showed that these inserts are essential for the catalytic activities of both the decarboxylase domains. Plasmodia conserved secondary structures within these inserts were identified and were also shown to be very important for domain activities, possibly through protein-protein interactions across and within the domains of the bifunctional complex for the efficient regulation of intracellular polyamine levels. The N-terminally located O1 insert in the PfODC domain is a highly conserved and structurally distinct insert, which is essential for both domain activities. Previous studies showed that the deletion of this insert prevents dimerisation of the PfODC monomers and as a result influences association of PfODC with the PfAdoMetDC domain to form the bifunctional ~330 kDa complex. In addition, immobilisation of the insert via the mutagenesis of flanking Gly residues and the disruption of a single conserved α-helix within the insert severely affected both PfODC and PfAdoMetDC activities. It was thus hypothesised that the helix is involved in protein-protein interactions and the dimerisation of the PfODC domain. Size-exclusion chromatography of the monofunctional PfODC and bifunctional PfAdoMetDC/ODC proteins with disrupted helices resulted in the elution of only the monomeric (~85 kDa) and heterodimeric PfAdoMetDC/ODC (~160 kDa) proteins, respectively. The mono- and bifunctional wild type and immobile proteins eluted as both dimeric PfODC (~170 kDa) and heterotetrameric (~330 kDa) fractions as a result of intact protein-protein interactions. These results were subsequently exploited in the… Advisors/Committee Members: Louw, Abraham Izak (advisor), Birkholtz, Lyn-Marie (advisor).

Subjects/Keywords: Eukaryotic cells; Polyamine metabolism; Malaria; Enzymes; UCTD

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APA (6^th Edition):

Williams, M. (2008). Delineation of functional roles of parasite-specific inserts in the malarial S-adenosylmethionine decarboxylase / ornithine decarboxylase . (Masters Thesis). University of Pretoria. Retrieved from http://upetd.up.ac.za/thesis/available/etd-08042008-072858/

Chicago Manual of Style (16^th Edition):

Williams, Marni. “Delineation of functional roles of parasite-specific inserts in the malarial S-adenosylmethionine decarboxylase / ornithine decarboxylase .” 2008. Masters Thesis, University of Pretoria. Accessed March 05, 2021. http://upetd.up.ac.za/thesis/available/etd-08042008-072858/.

MLA Handbook (7^th Edition):

Williams, Marni. “Delineation of functional roles of parasite-specific inserts in the malarial S-adenosylmethionine decarboxylase / ornithine decarboxylase .” 2008. Web. 05 Mar 2021.

Vancouver:

Williams M. Delineation of functional roles of parasite-specific inserts in the malarial S-adenosylmethionine decarboxylase / ornithine decarboxylase . [Internet] [Masters thesis]. University of Pretoria; 2008. [cited 2021 Mar 05]. Available from: http://upetd.up.ac.za/thesis/available/etd-08042008-072858/.

Council of Science Editors:

Williams M. Delineation of functional roles of parasite-specific inserts in the malarial S-adenosylmethionine decarboxylase / ornithine decarboxylase . [Masters Thesis]. University of Pretoria; 2008. Available from: http://upetd.up.ac.za/thesis/available/etd-08042008-072858/

University of Rochester

3. Ambeskovic, Aslihan. Essential Role for a Link between the Tricarboxylic Acid (TCA) Cycle and Polyamine Metabolism in Malignant Cell Transformation.

Degree: PhD, 2014, University of Rochester

URL: http://hdl.handle.net/1802/28951

► Malignant transformation requires reprogramming of the molecular network that underlies cell metabolism in order to convert available nutrients to biomass and energy to respond to… (more)

▼ Malignant transformation requires reprogramming of the molecular network that underlies cell metabolism in order to convert available nutrients to biomass and energy to respond to biological demands of proliferation and tumor microenvironment. In this context two apparently distinct metabolic pathways, glutamine-dependent anaplerosis and polyamine metabolism are commonly found up-regulated in cancer cells. Here we show that, increased glutamine-dependent anaplerosis in cancer cells is partly driven by increased polyamine metabolism. Dependency of anaplerosis on polyamine metabolism is observed when glutamine carbon is selected over arginine carbon to synthesize the polyamine precursor, ornithine. This unexpected relationship between polyamine and glutamine metabolism is shared by multiple cancer types and controlled by an ornithine aminotransferase (Oat)-driven transamination reaction. Oat uses L-glutamate-semialdehyde, a glutamate derived intermediate, as an amine group acceptor and glutamate as an amine group donor, producing ornithine and α-ketoglutarate (α-KG), with the latter fueling the TCA cycle. Genetic suppression of this metabolic link by targeting Oat leads to inhibition of tumor formation, down-regulation of glutamine-dependent anaplerosis and disruption of cellular redox homeostasis. Furthermore, down-regulation of polyamine metabolism by suppressing expression of spermine synthase (Sms), a polyamine biosynthetic enzyme that we find to be commonly up-regulated in multiple cancers compared to normal samples, also inhibits tumor growth. Metabolic analysis of cancer cells with Sms depletion shows that synthesis of ornithine and α-KG from glutamine is also decreased showing that increased polyamine metabolism is critical for glutamine-dependent anaplerosis of the TCA cycle in cancer cells. Overall, our findings demonstrate a direct link between polyamine metabolism and glutamine-dependent anaplerosis that is essential for the cancer phenotype. As this pathway is non-essential to normal cells, its aberrant up-regulation in cancer cells may provide novel opportunities for therapeutic intervention in cancer.

Subjects/Keywords: Cancer; Metabolism; Tricarboxylic Acid Cycle; Polyamine Metabolism

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APA (6^th Edition):

Ambeskovic, A. (2014). Essential Role for a Link between the Tricarboxylic Acid (TCA) Cycle and Polyamine Metabolism in Malignant Cell Transformation. (Doctoral Dissertation). University of Rochester. Retrieved from http://hdl.handle.net/1802/28951

Chicago Manual of Style (16^th Edition):

Ambeskovic, Aslihan. “Essential Role for a Link between the Tricarboxylic Acid (TCA) Cycle and Polyamine Metabolism in Malignant Cell Transformation.” 2014. Doctoral Dissertation, University of Rochester. Accessed March 05, 2021. http://hdl.handle.net/1802/28951.

MLA Handbook (7^th Edition):

Ambeskovic, Aslihan. “Essential Role for a Link between the Tricarboxylic Acid (TCA) Cycle and Polyamine Metabolism in Malignant Cell Transformation.” 2014. Web. 05 Mar 2021.

Vancouver:

Ambeskovic A. Essential Role for a Link between the Tricarboxylic Acid (TCA) Cycle and Polyamine Metabolism in Malignant Cell Transformation. [Internet] [Doctoral dissertation]. University of Rochester; 2014. [cited 2021 Mar 05]. Available from: http://hdl.handle.net/1802/28951.

Council of Science Editors:

Ambeskovic A. Essential Role for a Link between the Tricarboxylic Acid (TCA) Cycle and Polyamine Metabolism in Malignant Cell Transformation. [Doctoral Dissertation]. University of Rochester; 2014. Available from: http://hdl.handle.net/1802/28951

Temple University

4. Liu, Chunli. THE ROLE OF POLYAMINE ACETYLATION IN REGULATING ADIPOSE TISSUE METABOLISM.

Degree: PhD, 2011, Temple University

URL: http://digital.library.temple.edu/u?/p245801coll10,213123

►

Biochemistry

Because excessive body weight is a major health issue, there is an urgent need to understand all physiological mechanisms relating to control of fat… (more)

▼

Biochemistry

Because excessive body weight is a major health issue, there is an urgent need to understand all physiological mechanisms relating to control of fat deposition/mobilization. Here we investigated the linkage between polyamine metabolism and fat homeostasis that we recently discovered to operate in mice. Our previous data show that the expression level of spermine/spermidine acetyltransferase (SSAT), a polyamine catabolic enzyme, potently modulates body fat content of mice. In particular, our data indicated that SSAT overexpressing mice (SSAT-Tg) have reduced acetyl CoA levels and are lean while SSAT null mice (SSAT-ko) are obese. Since the acetyl CoA/malonyl CoA levels are critical for control of free fatty acid synthesis and oxidation via malonyl CoA regulation of CPT-1 (carnitine palmitoyltransferase-1), we hypothesized that genetic manipulation of SSAT alters body fat accumulation by activating of AMP-activated protein kinase pathway and thus has a global effect on energy metabolism. To test this hypothesis, we performed a combination of proteomics and antibody based expression studies in white adipose tissue (WAT) of SSAT-ko, SSAT-wt and SSAT-tg: We identified 9 proteins in WAT that show an increasing gradient across SSAT-ko, SSAT-wt and SSAT-tg, all of which have a connection with acetyl-CoA consumption. These include: a) glycolytic enzymes (aldolase, enolase, pyruvate dehydrogenase); b) TCA cycle enzymes (aconitate hydratase, malate dehydrogenase); c) fatty acid lipolysis and beta oxidation enzymes (hormone-sensitive lipase, monoglyceride lipase, 3-hydroxyacyl CoA dehydrogenase). Additional expression studies using Western blots indicated that acetyl CoA regulates metabolism by AMP-activated protein kinase pathway. Furthermore, to determine how tissue-specific changes in SSAT expression will impact fat accumulation and the precise role of SSAT expression status in fat homeostasis and obesity, we generated adipose-specific SAT1 knockout (FSAT1KO) mice using the Cre-Lox method. On 27-week-old, FSAT1KO mice have higher average body weight than wild type mice (WT: 45.13 ± 2.23 g vs. FSAT1KO: 52.28 ± 1.62 g, p<0.05) when fed a high-fat diet. Larger lipid droplets and lipid accumulation were present in FSAT1KO mouse livers compared to the control WT mice. Several proteins involved in fat metabolism were found to be up-regulated in FSAT1KO mice using GeLC-MS proteomics approach. These data indicated that the lack of SSAT activity in adipose tissue, but not liver or muscle, drives the phenotypic changes in SSAT-ko obese mice. Our interpretation of these results is that genetic modulation of SSAT causes a combination of changes in WAT that involve lipolysis, energy metabolism and calorie loss resulting from polyamine export. In summary, the data indicate that modulation of SSAT activity affects fat metabolism and calorie balance.

Temple University – Theses

Advisors/Committee Members: Merali, Salim, Chong, Parkson Lee-Gau, Collins, Jimmy H., Grubmeyer, Charles, Clarkson, Allen B..

Subjects/Keywords: Biochemistry; adipose tissue; metabolism; polyamine acetylation; SSAT

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Liu, C. (2011). THE ROLE OF POLYAMINE ACETYLATION IN REGULATING ADIPOSE TISSUE METABOLISM. (Doctoral Dissertation). Temple University. Retrieved from http://digital.library.temple.edu/u?/p245801coll10,213123

Chicago Manual of Style (16^th Edition):

Liu, Chunli. “THE ROLE OF POLYAMINE ACETYLATION IN REGULATING ADIPOSE TISSUE METABOLISM.” 2011. Doctoral Dissertation, Temple University. Accessed March 05, 2021. http://digital.library.temple.edu/u?/p245801coll10,213123.

MLA Handbook (7^th Edition):

Liu, Chunli. “THE ROLE OF POLYAMINE ACETYLATION IN REGULATING ADIPOSE TISSUE METABOLISM.” 2011. Web. 05 Mar 2021.

Vancouver:

Liu C. THE ROLE OF POLYAMINE ACETYLATION IN REGULATING ADIPOSE TISSUE METABOLISM. [Internet] [Doctoral dissertation]. Temple University; 2011. [cited 2021 Mar 05]. Available from: http://digital.library.temple.edu/u?/p245801coll10,213123.

Council of Science Editors:

Liu C. THE ROLE OF POLYAMINE ACETYLATION IN REGULATING ADIPOSE TISSUE METABOLISM. [Doctoral Dissertation]. Temple University; 2011. Available from: http://digital.library.temple.edu/u?/p245801coll10,213123

University of Pretoria

5. Williams, Marni. Delineation of functional roles of parasite-specific inserts in the malarial S-adenosylmethionine decarboxylase / ornithine decarboxylase.

Degree: Biochemistry, 2008, University of Pretoria

URL: http://hdl.handle.net/2263/26979

► The polyamines putrescine, spermidine and spermine play essential roles in the proliferation and differentiation of most eukaryotic cells. Inhibition of the polyamine pathway is known… (more)

▼ The polyamines putrescine, spermidine and spermine play essential roles in the proliferation and differentiation of most eukaryotic cells. Inhibition of the polyamine pathway is known to have antitumour and antiparasitic effects and á-difluoromethylornithine (DFMO), a polyamine biosynthesis inhibitor, is clinically used in the treatment of African sleeping sickness caused by Trypanosoma brucei gambiense. Ornithine decarboxylase (ODC) and Sadenosylmethionine decarboxylase (AdoMetDC) are the rate-limiting enzymes in polyamine metabolism. Usually, these enzymes are individually regulated, however, in the malaria parasite, Plasmodium falciparum, these enzymes are part of a unique bifunctional PfAdoMetDC/ODC protein. In addition, compared to homologous proteins, this malarial protein contains six unique parasite-specific inserted regions, which can be targeted with novel drugs. A modified restriction enzyme-mediated inverse PCR method was developed to delete the largest parasite-specific insert (411 bp) from the large PfAdoMetDC/ODC gene (4257 bp). The method was compared to existing deletion mutagenesis PCR protocols and was shown to be the most effective method (80% mutagenesis efficiency) as opposed to the 40% positively mutated clones obtained with the overlapping primer method in deleting a >100 bp region. The independent removal of all three the PfAdoMetDC domain parasite-specific inserts and subsequent activity analysis thereof showed that these inserts are essential for the catalytic activities of both the decarboxylase domains. Plasmodia conserved secondary structures within these inserts were identified and were also shown to be very important for domain activities, possibly through protein-protein interactions across and within the domains of the bifunctional complex for the efficient regulation of intracellular polyamine levels. The N-terminally located O1 insert in the PfODC domain is a highly conserved and structurally distinct insert, which is essential for both domain activities. Previous studies showed that the deletion of this insert prevents dimerisation of the PfODC monomers and as a result influences association of PfODC with the PfAdoMetDC domain to form the bifunctional ~330 kDa complex. In addition, immobilisation of the insert via the mutagenesis of flanking Gly residues and the disruption of a single conserved α-helix within the insert severely affected both PfODC and PfAdoMetDC activities. It was thus hypothesised that the helix is involved in protein-protein interactions and the dimerisation of the PfODC domain. Size-exclusion chromatography of the monofunctional PfODC and bifunctional PfAdoMetDC/ODC proteins with disrupted helices resulted in the elution of only the monomeric (~85 kDa) and heterodimeric PfAdoMetDC/ODC (~160 kDa) proteins, respectively. The mono- and bifunctional wild type and immobile proteins eluted as both dimeric PfODC (~170 kDa) and heterotetrameric (~330 kDa) fractions as a result of intact protein-protein interactions. These results were subsequently exploited in the… Advisors/Committee Members: Louw, Abraham Izak (advisor), Birkholtz, Lyn-Marie (advisor).

Subjects/Keywords: Eukaryotic cells; Polyamine metabolism; Malaria; Enzymes; UCTD

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Williams, M. (2008). Delineation of functional roles of parasite-specific inserts in the malarial S-adenosylmethionine decarboxylase / ornithine decarboxylase. (Masters Thesis). University of Pretoria. Retrieved from http://hdl.handle.net/2263/26979

Chicago Manual of Style (16^th Edition):

Williams, Marni. “Delineation of functional roles of parasite-specific inserts in the malarial S-adenosylmethionine decarboxylase / ornithine decarboxylase.” 2008. Masters Thesis, University of Pretoria. Accessed March 05, 2021. http://hdl.handle.net/2263/26979.

MLA Handbook (7^th Edition):

Williams, Marni. “Delineation of functional roles of parasite-specific inserts in the malarial S-adenosylmethionine decarboxylase / ornithine decarboxylase.” 2008. Web. 05 Mar 2021.

Vancouver:

Williams M. Delineation of functional roles of parasite-specific inserts in the malarial S-adenosylmethionine decarboxylase / ornithine decarboxylase. [Internet] [Masters thesis]. University of Pretoria; 2008. [cited 2021 Mar 05]. Available from: http://hdl.handle.net/2263/26979.

Council of Science Editors:

Williams M. Delineation of functional roles of parasite-specific inserts in the malarial S-adenosylmethionine decarboxylase / ornithine decarboxylase. [Masters Thesis]. University of Pretoria; 2008. Available from: http://hdl.handle.net/2263/26979

University of Otago

6. Anderson, Cory Robert. The effects of ultraviolet-B deficient conditions on antioxidant metabolism and polyamine accumulation in lettuce (Lactuca sativa L.) .

Degree: 2012, University of Otago

URL: http://hdl.handle.net/10523/2376

► All light from the sun contains radiation in the ultraviolet (UV) range, wavelengths between 200 and 400 nm that may be harmful to living organisms.… (more)

▼ All light from the sun contains radiation in the ultraviolet (UV) range, wavelengths between 200 and 400 nm that may be harmful to living organisms. Exposure of plant tissues to UV-B radiation (200-400 nm) may result in the formation of reactive oxygen species (ROS), highly reactive chemical species that are capable of damaging biological macromolecules such as DNA, proteins and the lipids of cellular membranes. To detoxify ROS and prevent damage from occurring, plants maintain a battery of antioxidants and associated enzymes. Polyamines are small, aliphatic amines that are found in plants that also function in the stress response by protecting DNA, stabilizing cellular macromolecules and aiding the dissipation of excess energy in photosystem II (PSII). Antioxidants and polyamines are also important in human metabolism and may play a role in preventing the development of several chronic diseases including cancer, diabetes and cardiovascular disease. As antioxidant compounds and polyamines are accumulated in plants under conditions of UV-B stress, there is scope to increase the nutritional value of plant foods by exposing crops to UV-B. The aim of the current experiments was to investigate the effects of UV-B on the activity of antioxidants and polyamine accumulation in lettuce (Lactuca sativa L.), a leafy vegetable commonly grown in horticultural set-ups that reduce UV-B exposure. The ability of plant material from different UV-B environments to protect human colon cells from oxidative injury was also investigated. Exposure to UV-B increased the activity of the antioxidative enzymes superoxide dismutase, catalase, ascorbate peroxidase, glutathione peroxidase and glutathione reductase as well as increasing the accumulation of the low-molecular weight antioxidants ascorbate and glutathione. Oxidative damage in UV-B exposed plants was reflected in increases in protein carbonyl and lipid hydroperoxide contents, and in increased oxidation of cellular ascorbate and glutathione pools. However, plants acclimatized to UV-B as the experiment progressed with markers of oxidative damage decreasing after one week of exposure. The response of lettuces to UV-B radiation also varied between varieties, the red-leafed cultivar ‘Red Salad Bowl’ having lower levels of oxidative damage and recovering more fully than other cultivars. Polyamines were also accumulated in response to UV-B radiation, especially free and conjugated forms putrescine and spermidine. Accumulation of spermine however, increased as UV-B exposure progressed and a higher proportion of spermine was accumulated as bound-spermine than was the case for other polyamines. Two pathways for polyamine biosynthesis exist in plants, starting from either ornithine decarboxylase (ODC) or arginine decarboxylase (ADC). ODC activity was not altered by UV-B. ADC activity was up-regulated in UV-B exposed plants, and the localization of this enzyme in chloroplasts suggests a role for polyamines in stabilizing PSII during UV-B stress. The accumulation of antioxidants… Advisors/Committee Members: Burritt, David J (advisor).

Subjects/Keywords: ultraviolet; antioxidant; polyamine; lettuce; Caco-2

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APA (6^th Edition):

Anderson, C. R. (2012). The effects of ultraviolet-B deficient conditions on antioxidant metabolism and polyamine accumulation in lettuce (Lactuca sativa L.) . (Masters Thesis). University of Otago. Retrieved from http://hdl.handle.net/10523/2376

Chicago Manual of Style (16^th Edition):

Anderson, Cory Robert. “The effects of ultraviolet-B deficient conditions on antioxidant metabolism and polyamine accumulation in lettuce (Lactuca sativa L.) .” 2012. Masters Thesis, University of Otago. Accessed March 05, 2021. http://hdl.handle.net/10523/2376.

MLA Handbook (7^th Edition):

Anderson, Cory Robert. “The effects of ultraviolet-B deficient conditions on antioxidant metabolism and polyamine accumulation in lettuce (Lactuca sativa L.) .” 2012. Web. 05 Mar 2021.

Vancouver:

Anderson CR. The effects of ultraviolet-B deficient conditions on antioxidant metabolism and polyamine accumulation in lettuce (Lactuca sativa L.) . [Internet] [Masters thesis]. University of Otago; 2012. [cited 2021 Mar 05]. Available from: http://hdl.handle.net/10523/2376.

Council of Science Editors:

Anderson CR. The effects of ultraviolet-B deficient conditions on antioxidant metabolism and polyamine accumulation in lettuce (Lactuca sativa L.) . [Masters Thesis]. University of Otago; 2012. Available from: http://hdl.handle.net/10523/2376

7. Lilley, Graham R. Characterisation of a calcium dependent transglutaminase in Pisum sativum leaf and root tissue.

Degree: PhD, 1999, Nottingham Trent University

URL: http://irep.ntu.ac.uk/id/eprint/41108/ ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.285650

Subjects/Keywords: 572; Polyamine incorporation

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APA (6^th Edition):

Lilley, G. R. (1999). Characterisation of a calcium dependent transglutaminase in Pisum sativum leaf and root tissue. (Doctoral Dissertation). Nottingham Trent University. Retrieved from http://irep.ntu.ac.uk/id/eprint/41108/ ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.285650

Chicago Manual of Style (16^th Edition):

Lilley, Graham R. “Characterisation of a calcium dependent transglutaminase in Pisum sativum leaf and root tissue.” 1999. Doctoral Dissertation, Nottingham Trent University. Accessed March 05, 2021. http://irep.ntu.ac.uk/id/eprint/41108/ ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.285650.

MLA Handbook (7^th Edition):

Lilley, Graham R. “Characterisation of a calcium dependent transglutaminase in Pisum sativum leaf and root tissue.” 1999. Web. 05 Mar 2021.

Vancouver:

Lilley GR. Characterisation of a calcium dependent transglutaminase in Pisum sativum leaf and root tissue. [Internet] [Doctoral dissertation]. Nottingham Trent University; 1999. [cited 2021 Mar 05]. Available from: http://irep.ntu.ac.uk/id/eprint/41108/ ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.285650.

Council of Science Editors:

Lilley GR. Characterisation of a calcium dependent transglutaminase in Pisum sativum leaf and root tissue. [Doctoral Dissertation]. Nottingham Trent University; 1999. Available from: http://irep.ntu.ac.uk/id/eprint/41108/ ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.285650

Georgia State University

8. Peng, Yu-chih. Molecular Mechanism of Pseudmonas Aeruginosa Responses to Spermine Stress.

Degree: PhD, Biology, 2016, Georgia State University

URL: https://scholarworks.gsu.edu/biology_diss/179

► Pseudomonas aeruginosa can grow efficiently on spermine and other biogenic polyamines via the γ-glutamylpolyamine synthetase (GPS) pathway. Not only subjected to growth inhibition by… (more)

▼ Pseudomonas aeruginosa can grow efficiently on spermine and other biogenic polyamines via the γ-glutamylpolyamine synthetase (GPS) pathway. Not only subjected to growth inhibition by spermine, the pauA2 mutant without a functional γ-glutamylpolyamine synthetase PauA2 became more sensitive to β-lactam antibiotics in human serum. To explore PauA2 as a potential target of drug development, the native form of PauA2 protein overexpressed in E. coli was purified to homogeneity for biochemical characterization. The specific activity of PauA2 was monitored by spectrophotometric measurements (660 nm) of the releasing phosphate from ATP in the presence of ammonium molybdate and malachite green. PauA2 displayed a sigmoid curve on Velocity-Concentration plot, indicating an allosteric modulation in the catalytic reaction. The apparent Km values were 0.2mM, 2.1mM, 6.1mM, and 1.1mM for ATP, L-glutamate, spermidine, and spermine, respectively. The obtained values of Hill coefficient were 3.4 and 5.4 for spermidine and spermine, respectively. Although P. aeruginosa can degrade the spermine by PauA2, it seems likely that other mechanisms may alleviate the spermine toxicity in the absence of pauA2. All the pauA2 suppressors were isolated from spermine selection plates and shared common changes in various pathways including delayed growth rate, retarded swarming motility, and pyocyanin overproduction. Genome resequencing of a representative suppressor revealed a unique C₅₉₉T mutation at the phoU gene that results in Ser₂₀₀Leu substitution and a constitutive expression of the Pho regulon as evidenced by measurements of promotor activities and transcriptome analysis. All of the observed phenotypes could be complemented by a recombinant plasmid carrying the wild-type phoU gene. Also, accumulation of polyphosphate granules and spermine resistance in the suppressor mutant were reversed concomitantly when exopolyphosphatase PPX was overexpressed from a recombinant plasmid. Identical phenotypes were also observed in a ΔpauA2ΔphoU double knockout mutant. In conclusion, we characterized the γ-glutamylspermine synthetase PauA2 as the essential enzyme and provide the foundations for PauA2 inhibitors screening as a potential antibacterial. Furthermore, we identified polyphosphate accumulation as a potential protection mechanism against spermine toxicity in P. aeruginosa. Advisors/Committee Members: Chung-Dar Lu, Zehava Eichenbaum, Irene Weber.

Subjects/Keywords: Spermine; PHO regulon; Polyphosphate; PhoU; PauA2; Polyamine

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APA (6^th Edition):

Peng, Y. (2016). Molecular Mechanism of Pseudmonas Aeruginosa Responses to Spermine Stress. (Doctoral Dissertation). Georgia State University. Retrieved from https://scholarworks.gsu.edu/biology_diss/179

Chicago Manual of Style (16^th Edition):

Peng, Yu-chih. “Molecular Mechanism of Pseudmonas Aeruginosa Responses to Spermine Stress.” 2016. Doctoral Dissertation, Georgia State University. Accessed March 05, 2021. https://scholarworks.gsu.edu/biology_diss/179.

MLA Handbook (7^th Edition):

Peng, Yu-chih. “Molecular Mechanism of Pseudmonas Aeruginosa Responses to Spermine Stress.” 2016. Web. 05 Mar 2021.

Vancouver:

Peng Y. Molecular Mechanism of Pseudmonas Aeruginosa Responses to Spermine Stress. [Internet] [Doctoral dissertation]. Georgia State University; 2016. [cited 2021 Mar 05]. Available from: https://scholarworks.gsu.edu/biology_diss/179.

Council of Science Editors:

Peng Y. Molecular Mechanism of Pseudmonas Aeruginosa Responses to Spermine Stress. [Doctoral Dissertation]. Georgia State University; 2016. Available from: https://scholarworks.gsu.edu/biology_diss/179

Georgia State University

9. Nguyen, Khoa. An Investigation of the DNA Interactions of Polyamine Anthracene Conjugates under High Ionic Conditions.

Degree: MS, Chemistry, 2016, Georgia State University

URL: https://scholarworks.gsu.edu/chemistry_theses/95

► Six polyamine anthracene conjugates (Ants) were studied that take advantage of the polyamine transporter system (PTS) to target specific cancer. The structural features of… (more)

Subjects/Keywords: DNA; ionic condition; polyamine anthracene conjugate

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Nguyen, K. (2016). An Investigation of the DNA Interactions of Polyamine Anthracene Conjugates under High Ionic Conditions. (Thesis). Georgia State University. Retrieved from https://scholarworks.gsu.edu/chemistry_theses/95

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Nguyen, Khoa. “An Investigation of the DNA Interactions of Polyamine Anthracene Conjugates under High Ionic Conditions.” 2016. Thesis, Georgia State University. Accessed March 05, 2021. https://scholarworks.gsu.edu/chemistry_theses/95.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Nguyen, Khoa. “An Investigation of the DNA Interactions of Polyamine Anthracene Conjugates under High Ionic Conditions.” 2016. Web. 05 Mar 2021.

Vancouver:

Nguyen K. An Investigation of the DNA Interactions of Polyamine Anthracene Conjugates under High Ionic Conditions. [Internet] [Thesis]. Georgia State University; 2016. [cited 2021 Mar 05]. Available from: https://scholarworks.gsu.edu/chemistry_theses/95.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Nguyen K. An Investigation of the DNA Interactions of Polyamine Anthracene Conjugates under High Ionic Conditions. [Thesis]. Georgia State University; 2016. Available from: https://scholarworks.gsu.edu/chemistry_theses/95

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Tampere University

10. Fashe, Tekele Markos. Regulation of Hematopoietic Cell Development and Hair Growth in Mouse Models: Expression Analysis of Tudor-SN and Polyamine-Regulated Proteins .

Degree: 2013, Tampere University

URL: https://trepo.tuni.fi/handle/10024/94639

► Malliorganismeiksi kutsutaan eliöitä, joilla voidaan mallintaa ihmisessä tapahtuvia fysiologisia toimintoja ja sairauksia. Tässä väitöskirjatutkimuksessa käytettiin hiirimallia selvitettäessä Tudor-SN ja polyamiini-säädeltyjen proteiinien ilmentymistä ja toimintaa. Tudor-SN… (more)

▼ Malliorganismeiksi kutsutaan eliöitä, joilla voidaan mallintaa ihmisessä tapahtuvia fysiologisia toimintoja ja sairauksia. Tässä väitöskirjatutkimuksessa käytettiin hiirimallia selvitettäessä Tudor-SN ja polyamiini-säädeltyjen proteiinien ilmentymistä ja toimintaa. Tudor-SN proteiinille on ehdotettu useita mekanismeja geenien ilmentymisen säätelijänä. Kuitenkaan itse Tudor-SN proteiinin ilmentymistä eri kudoksissa ei ole tarkoin tutkittu ja proteiinin fysiologinen merkitys on epäselvä. Tutkimuksessa selvisi, että Tudor-SN ilmentyy erityisesti nopeasti jakaantuvissa soluissa ja erilaistuvien solutyyppien jakaantuvissa esiastesoluissa. Tudor-SN poistogeenisen hiiren analyysissä todettiin sekä luuytimessä että verenkierrossa alentunut granulosyytti-solujen granulaarisuus sekä alentunut granulosyyttien määrä verenkierrossa. Tulokset ehdottavat, että Tudor-SN proteiini on merkityksellinen immuunijärjestelmässä. Luonnon polyamiineista putreskiini, spermidiini ja spermiini ovat ratkaisevia nisäkkäiden solujen erilaistumisessa ja lisääntymisessä. Tutkimuksessa selvitettiin polyamiinien vaikutusta karvan kasvuun, joka tapahtuu kolmessa eri vaiheessa: nopea solujakaantuminen (anagen), apoptoosi (catagen) ja loppuvaiheen lepotila (telogen). Anagen-vaiheessa olevissa karvasoluissa on raportoitu korkea polyamiinipitoisuus. Tässä tutkimuksessa selvitettiin metabolisesti stabiilin polyymiinianalogin, alpha-metyylispermidiinin vaikutus karvan kasvuun. Kahden viikon päivittäinen alpha-metyylispermidiini annostelu telogen-vaiheessa oleville hiirillä aikaansai uudelleen nopean solujakaantumisen, eli anagen-vaiheen. Tulosten mukaan polyamiineilla voidaan vaikuttaa karvan kasvuun hiirimallissa.; Model organisms or reference organisms are non-human species, which are used to explore a given biological phenomenon deliberately to deduce the anticipated experimental findings to other organisms particularly to humans. Hypothetically, a given organism can be considered as a model organism when its size is small and it is competent to represent a certain specific organism by predicting myriads of biological and molecular processes related to genetics, development, physiology, evolution and ecology. By taking genetic conservation into account, a model organism with small size or simple form of life is expected to represent a larger organism including humans with complex genome and biological processes. Although no single organisms fulfill these theoretical criteria, most of our current knowledge of heredity, development, physiology and underlining molecular and cellular processes is obtained from studies based on model organisms. In this study, by using mouse as a model organism expression of Tudor-SN and polyamine regulated proteins were analyzed. Tudor-SN staphylococcal nuclease (Tudor-SN) is a 100 kDa protein that was initially identified as a transcriptional co-activator. It has also been shown to function as a modulator of RNA metabolism and biogenesis and a component in the RNA-induced silencing complex (RISC). Tudor-SN protein…

Subjects/Keywords: Gene Expression ; Hematopoiesis ; Mouse Model ; Polyamine

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Fashe, T. M. (2013). Regulation of Hematopoietic Cell Development and Hair Growth in Mouse Models: Expression Analysis of Tudor-SN and Polyamine-Regulated Proteins . (Doctoral Dissertation). Tampere University. Retrieved from https://trepo.tuni.fi/handle/10024/94639

Chicago Manual of Style (16^th Edition):

Fashe, Tekele Markos. “Regulation of Hematopoietic Cell Development and Hair Growth in Mouse Models: Expression Analysis of Tudor-SN and Polyamine-Regulated Proteins .” 2013. Doctoral Dissertation, Tampere University. Accessed March 05, 2021. https://trepo.tuni.fi/handle/10024/94639.

MLA Handbook (7^th Edition):

Fashe, Tekele Markos. “Regulation of Hematopoietic Cell Development and Hair Growth in Mouse Models: Expression Analysis of Tudor-SN and Polyamine-Regulated Proteins .” 2013. Web. 05 Mar 2021.

Vancouver:

Fashe TM. Regulation of Hematopoietic Cell Development and Hair Growth in Mouse Models: Expression Analysis of Tudor-SN and Polyamine-Regulated Proteins . [Internet] [Doctoral dissertation]. Tampere University; 2013. [cited 2021 Mar 05]. Available from: https://trepo.tuni.fi/handle/10024/94639.

Council of Science Editors:

Fashe TM. Regulation of Hematopoietic Cell Development and Hair Growth in Mouse Models: Expression Analysis of Tudor-SN and Polyamine-Regulated Proteins . [Doctoral Dissertation]. Tampere University; 2013. Available from: https://trepo.tuni.fi/handle/10024/94639

University of California – Riverside

11. Gil, Jacqueline Michelle. Regulation of Polyamine Synthesis and Transport in the Context of Macrophage Polarization and Pathogen Invasion.

Degree: Biomedical Sciences, 2014, University of California – Riverside

URL: http://www.escholarship.org/uc/item/3gk6k81k

► Macrophages play a vital role in early defense against pathogens and are required for tissue repair following injury. In response to various stimuli, macrophages become… (more)

Subjects/Keywords: Medicine; Biochemistry; Cellular biology; arginine/polyamine transport; leishmania; macrophage activation/polarization; macrophages; polyamine inhibitors; polyamines

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Gil, J. M. (2014). Regulation of Polyamine Synthesis and Transport in the Context of Macrophage Polarization and Pathogen Invasion. (Thesis). University of California – Riverside. Retrieved from http://www.escholarship.org/uc/item/3gk6k81k

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Gil, Jacqueline Michelle. “Regulation of Polyamine Synthesis and Transport in the Context of Macrophage Polarization and Pathogen Invasion.” 2014. Thesis, University of California – Riverside. Accessed March 05, 2021. http://www.escholarship.org/uc/item/3gk6k81k.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Gil, Jacqueline Michelle. “Regulation of Polyamine Synthesis and Transport in the Context of Macrophage Polarization and Pathogen Invasion.” 2014. Web. 05 Mar 2021.

Vancouver:

Gil JM. Regulation of Polyamine Synthesis and Transport in the Context of Macrophage Polarization and Pathogen Invasion. [Internet] [Thesis]. University of California – Riverside; 2014. [cited 2021 Mar 05]. Available from: http://www.escholarship.org/uc/item/3gk6k81k.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Gil JM. Regulation of Polyamine Synthesis and Transport in the Context of Macrophage Polarization and Pathogen Invasion. [Thesis]. University of California – Riverside; 2014. Available from: http://www.escholarship.org/uc/item/3gk6k81k

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

12. Jagu, Elodie. Design, synthesis and biological evaluation of new polyamine derivatives as antikinetoplastid agents : Synthèse et évaluation biologique de dérivés polyamines en tant qu’agents antikinétoplastidés.

Degree: Docteur es, Chimie, 2016, Université Paris-Saclay (ComUE)

URL: http://www.theses.fr/2016SACLS589

►

Ce projet d’interface Chimie/Biologie repose sur les expertises complémentaires de deux équipes. Il concerne la conception et le développement d’inhibiteurs dirigés contre les Kinétoplastidés (trypanosomes,… (more)

▼

Ce projet d’interface Chimie/Biologie repose sur les expertises complémentaires de deux équipes. Il concerne la conception et le développement d’inhibiteurs dirigés contre les Kinétoplastidés (trypanosomes, leishmanies). Il est en effet urgent de développer de nouvelles stratégies thérapeutiques pour répondre à la chimiorésistance et à la toxicité des médicaments actuellement utilisés contre ces parasites. Le métabolisme et le transport des polyamines étant essentiel chez les parasites, ils constituent des cibles thérapeutiques d’intérêt contre les Kinétoplastidés. Le projet intègre la synthèse de nouveaux dérivés polyamines spécifiques des parasites, l’évaluation sur des modèles in vitro de leishmaniose et de trypanosomose africaine, ainsi qu’une évaluation sur trypanothione réductase. La mise au point d’une méthode de quantification du transport de polyamine a également été initiée. Cinquante-quatre composés, répartis en trois séries chimiques, ont été synthétisés et évalués. Un grand nombre d’entre eux présentent des activités antiparasitaires de l’ordre du micromolaire et des évaluations in vivo sont actuellement en cours avec le composé le plus prometteur.

This project is at the interface of chemistry and biology and relies on the expertise of two different teams. This thesis involves the design and development of inhibitors directed against Kinetoplastids. It is urgent to develop new therapeutic strategies to respond to drug resistance and toxicity of currently used drugs against these parasites. Polyamine metabolism and transporter have been demonstrated as essential for parasite growth. Therefore, these systems are potential drug targets for development of antikinetoplastid compounds. We chose to synthesize polyamine derivatives and evaluate their biological activity against Kinetoplatids. Fifty-four compounds, divided into three chemical series, have been synthesized and evaluated. Many have shown a micromolar biological activity in vitro against parasite. In vivo evaluation is foreseen for the most promising derivative.

Advisors/Committee Members: Loiseau, Philippe (thesis director), Blonsky, Casimir (thesis director).

Subjects/Keywords: Polyamine; Kinetoplastidés; Trypanosoma; Leishmania; Chimie médicinale; Polyamine; Kinetoplastid; Trypanosoma; Leishmania; Medicinal Chemistry

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Jagu, E. (2016). Design, synthesis and biological evaluation of new polyamine derivatives as antikinetoplastid agents : Synthèse et évaluation biologique de dérivés polyamines en tant qu’agents antikinétoplastidés. (Doctoral Dissertation). Université Paris-Saclay (ComUE). Retrieved from http://www.theses.fr/2016SACLS589

Chicago Manual of Style (16^th Edition):

Jagu, Elodie. “Design, synthesis and biological evaluation of new polyamine derivatives as antikinetoplastid agents : Synthèse et évaluation biologique de dérivés polyamines en tant qu’agents antikinétoplastidés.” 2016. Doctoral Dissertation, Université Paris-Saclay (ComUE). Accessed March 05, 2021. http://www.theses.fr/2016SACLS589.

MLA Handbook (7^th Edition):

Jagu, Elodie. “Design, synthesis and biological evaluation of new polyamine derivatives as antikinetoplastid agents : Synthèse et évaluation biologique de dérivés polyamines en tant qu’agents antikinétoplastidés.” 2016. Web. 05 Mar 2021.

Vancouver:

Jagu E. Design, synthesis and biological evaluation of new polyamine derivatives as antikinetoplastid agents : Synthèse et évaluation biologique de dérivés polyamines en tant qu’agents antikinétoplastidés. [Internet] [Doctoral dissertation]. Université Paris-Saclay (ComUE); 2016. [cited 2021 Mar 05]. Available from: http://www.theses.fr/2016SACLS589.

Council of Science Editors:

Jagu E. Design, synthesis and biological evaluation of new polyamine derivatives as antikinetoplastid agents : Synthèse et évaluation biologique de dérivés polyamines en tant qu’agents antikinétoplastidés. [Doctoral Dissertation]. Université Paris-Saclay (ComUE); 2016. Available from: http://www.theses.fr/2016SACLS589

13. Auayporn, Apirakaramwong. MECHANISM OF CELL DEATH BY POLYAMINE ACCUMULATION.

Degree: Chiba University / 千葉大学

URL: http://opac.ll.chiba-u.jp/da/curator/900022532/

►

研究科: 千葉大学大学院薬学研究科

学位:千大院薬博甲第106号

抄録:The mechanism of cell death by polyamine accumulation has been studied. The accumulation of spermidine leads to a decrease in cell viability… (more)

Subjects/Keywords: Cell death; polyamine; Escherichia coli

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APA (6^th Edition):

Auayporn, A. (n.d.). MECHANISM OF CELL DEATH BY POLYAMINE ACCUMULATION. (Thesis). Chiba University / 千葉大学. Retrieved from http://opac.ll.chiba-u.jp/da/curator/900022532/

Note: this citation may be lacking information needed for this citation format:
No year of publication.
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Auayporn, Apirakaramwong. “MECHANISM OF CELL DEATH BY POLYAMINE ACCUMULATION.” Thesis, Chiba University / 千葉大学. Accessed March 05, 2021. http://opac.ll.chiba-u.jp/da/curator/900022532/.

Note: this citation may be lacking information needed for this citation format:
No year of publication.
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Auayporn, Apirakaramwong. “MECHANISM OF CELL DEATH BY POLYAMINE ACCUMULATION.” Web. 05 Mar 2021.

Note: this citation may be lacking information needed for this citation format:
No year of publication.

Vancouver:

Auayporn A. MECHANISM OF CELL DEATH BY POLYAMINE ACCUMULATION. [Internet] [Thesis]. Chiba University / 千葉大学; [cited 2021 Mar 05]. Available from: http://opac.ll.chiba-u.jp/da/curator/900022532/.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
No year of publication.

Council of Science Editors:

Auayporn A. MECHANISM OF CELL DEATH BY POLYAMINE ACCUMULATION. [Thesis]. Chiba University / 千葉大学; Available from: http://opac.ll.chiba-u.jp/da/curator/900022532/

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
No year of publication.

14. 富取, 秀行. 原核・真核細胞におけるポリアミン輸送蛋白質に関する研究 : Polyamine transport protein in E.coli and yeast.

Degree: Chiba University / 千葉大学

URL: http://opac.ll.chiba-u.jp/da/curator/900022604/

研究科: 千葉大学大学院薬学研究科

学位:千大院薬博甲第薬137号

抄録:【序論】ポリアミン(プトレスシン、スペルミジン、スペルミン)は、大腸菌からヒトまで普遍的に存在する生理活性アミンである。その生理的役割は多岐にわたるが、主に核酸、特にRNAと強く相互作用することにより、蛋白質および核酸の合成を促進し、細胞増殖因子として機能することが知られている。ポリアミンの細胞内濃度は生合成、分解、外界からの取り込み、および排出により厳密に調節されている。大腸菌のポリアミン輸送系は現在までに3種類見つかっており、2種類の取り込み系と1種類の排出系から構成されている。…

Subjects/Keywords: polyamine; transport; ポリアミン; 輸送

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APA (6^th Edition):

富取, �. (n.d.). 原核・真核細胞におけるポリアミン輸送蛋白質に関する研究 : Polyamine transport protein in E.coli and yeast. (Thesis). Chiba University / 千葉大学. Retrieved from http://opac.ll.chiba-u.jp/da/curator/900022604/

Note: this citation may be lacking information needed for this citation format:
No year of publication.
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

富取, 秀行. “原核・真核細胞におけるポリアミン輸送蛋白質に関する研究 : Polyamine transport protein in E.coli and yeast.” Thesis, Chiba University / 千葉大学. Accessed March 05, 2021. http://opac.ll.chiba-u.jp/da/curator/900022604/.

Note: this citation may be lacking information needed for this citation format:
No year of publication.
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

富取, 秀行. “原核・真核細胞におけるポリアミン輸送蛋白質に関する研究 : Polyamine transport protein in E.coli and yeast.” Web. 05 Mar 2021.

Note: this citation may be lacking information needed for this citation format:
No year of publication.

Vancouver:

富取 �. 原核・真核細胞におけるポリアミン輸送蛋白質に関する研究 : Polyamine transport protein in E.coli and yeast. [Internet] [Thesis]. Chiba University / 千葉大学; [cited 2021 Mar 05]. Available from: http://opac.ll.chiba-u.jp/da/curator/900022604/.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
No year of publication.

Council of Science Editors:

富取 �. 原核・真核細胞におけるポリアミン輸送蛋白質に関する研究 : Polyamine transport protein in E.coli and yeast. [Thesis]. Chiba University / 千葉大学; Available from: http://opac.ll.chiba-u.jp/da/curator/900022604/

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
No year of publication.

University of Otago

15. Gupta, Neeraj. Polyamines and Age-Related Cognitive Decline .

Degree: 2012, University of Otago

URL: http://hdl.handle.net/10523/2342

► Aging is a multi-factorial process and leads to cognitive decline. Such age-related cognitive decline is associated with dysfunction of the hippocampus and prefrontal cortex (PFC).… (more)

▼ Aging is a multi-factorial process and leads to cognitive decline. Such age-related cognitive decline is associated with dysfunction of the hippocampus and prefrontal cortex (PFC). Polyamines putrescine, spermidine and spermine are the down-stream metabolites of L-arginine, and have important roles in maintaining normal cellular function, regulating neurotransmitter receptors and modulating learning and memory. There is evidence suggesting an important role of putrescine in neurogenesis in the dentate gyrus (DG) of the hippocampus that is severely impaired during aging. The present thesis systematically investigated: (i) the effects of aging on behavioural function (including learning and memory) and polyamines and their main biosynthesis and regulatory enzymes in the sub-regions of the hippocampus and PFC (Experiments 1 and 2); and (ii) the behavioural effects of acute and chronic depletion of putrescine by alpha-difluoromethylornithine (DFMO) (Experiments 3 and 4), through combined behavioural, neurochemical and molecular biological approaches. Experiment 1 quantified the tissue concentrations of polyamines in the CA1, CA2/3 and DG sub-regions of the hippocampus and PFC from two sets of 4 (young), 12 (middle-aged) and 24 (aged) months old Sprague-Dawley (SD) rats without (Set I) and with (Set II) behavioural tests in the elevated plus maze, open field, water maze and object recognition memory tasks. There were reduced anxiety level and exploratory activity, and impaired spatial learning and memory with aging. Putrescine levels were significantly decreased in the CA1, DG and PFC, but increased in the CA2/3, with age, whereas there were significantly increased levels of spermidine in the CA1, CA2/3 and PFC, and spermine in the PFC, with age. Animals’ behavioral experience had little influence on the overall pattern of age-related changes in polyamines. It has been documented that behavioural performance and lifespan vary across different strains of rats. Hence Experiment 2 investigated the effects of aging on behavioural performance and polyamines and their main biosynthesis and regulatory enzymes in Long-Evans (LE) rats using the experimental protocols for Set II animals in Experiment 1. Although there were slightly reduced anxiety level and exploratory and locomotor activities with aging, no marked age-related spatial learning and memory deficits were observed, which are different from SD rats. The levels of putrescine, spermidine and spermine in the sub-regions of the hippocampus and PFC were altered with age in a region-specific manner, however with different patterns when compared to SD rats. Western blot revealed increased protein expression of ornithine decarboxylase (ODC, the key biosynthesis enzyme of putrescine) and antizyme inhibitor (an enzyme that regulates ODC activity indirectly) with age in a region-specific manner, with no age-related changes in antizyme (an enzyme that inhibits ODC). Collectively, the results of Experiments 1 and 2 demonstrate altered polyamine system in the hippocampus and… Advisors/Committee Members: Liu, Ping (advisor).

Subjects/Keywords: Polyamine; cognition; difluoromethylornithine (DFMO); putrescine; spermidine; spermine; aging; memory; hippocampus

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Gupta, N. (2012). Polyamines and Age-Related Cognitive Decline . (Doctoral Dissertation). University of Otago. Retrieved from http://hdl.handle.net/10523/2342

Chicago Manual of Style (16^th Edition):

Gupta, Neeraj. “Polyamines and Age-Related Cognitive Decline .” 2012. Doctoral Dissertation, University of Otago. Accessed March 05, 2021. http://hdl.handle.net/10523/2342.

MLA Handbook (7^th Edition):

Gupta, Neeraj. “Polyamines and Age-Related Cognitive Decline .” 2012. Web. 05 Mar 2021.

Vancouver:

Gupta N. Polyamines and Age-Related Cognitive Decline . [Internet] [Doctoral dissertation]. University of Otago; 2012. [cited 2021 Mar 05]. Available from: http://hdl.handle.net/10523/2342.

Council of Science Editors:

Gupta N. Polyamines and Age-Related Cognitive Decline . [Doctoral Dissertation]. University of Otago; 2012. Available from: http://hdl.handle.net/10523/2342

University of Pretoria

16. [No author]. Metabolomic analyses of the malaria parasite after inhibition of polyamine biosynthesis .

Degree: 2009, University of Pretoria

URL: http://upetd.up.ac.za/thesis/available/etd-10072009-162558/

► Malaria, a disease transmitted by female mosquitoes, has plagued the world for many centuries. The disease is associated with high mortality rates, severe poverty, and… (more)

▼ Malaria, a disease transmitted by female mosquitoes, has plagued the world for many centuries. The disease is associated with high mortality rates, severe poverty, and economic burden. These are factors which hamper effective eradication of the disease. Drug resistant forms of the parasite have caused increasing concerns and questioned the longevity of current effective antimalarials. Efforts are therefore aimed at the identification and exploitation of essential parasite proteins as potential drug targets. The polyamine pathway of Plasmodium falciparum is an exploitable pathway which contains two distinct, chemically validated drug targets; a bifunctional PfAdoMetDC-ODC protein and PfSpdSyn. These enzymes ensure intricate regulation of polyamine production and the pathway contains various distinctive features which could be selectively targetable from the mammalian counterpart pathways. However, inhibition of polyamine production through the use of specific enzyme inhibitors has revealed various compensatory responses that negate the efficacy of these inhibitors. An account is given of the metabolomic fluctuations in the parasite during inhibition of polyamine biosynthesis. From co-inhibited P. falciparum extracts, it could be demonstrated that the characteristic growth-arrest coincided with the depletion in spermidine, the metabolic product of PfSpdSyn. The co-inhibition strategy therefore emphasised the importance of spermidine biosynthesis by PfSpdSyn. Moreover, adenosyl-related metabolite levels were not disrupted during polyamine depletion, supporting the notion that these metabolites are intricately recycled within the parasites. The identified metabolic compensatory mechanisms have further potential for exploitation, and can strategically be combined with polyamine biosynthesis inhibition to ensure parasitic attenuation. In addition, several novel inhibitors were previously computationally identified, based on a dynamic receptor-based pharmacophore model of PfSpdSyn. The in vitro inhibiting activity of these compounds was determined against PfSpdSyn. Results from the in vitro experiments supported the in silico predictions, and emphasized the supportive role of pharmacophore modelling has for the identification of novel inhibitors. The research contributed in understanding parasitic polyamine metabolite regulation, and will aid in the future optimization of therapeutic strategies, aimed at exploitation of the polyamine pathway as a potential antimalarial drug target. Copyright Advisors/Committee Members: Birkholtz, Lyn-Marie (advisor), Louw, Abraham Izak (advisor).

Subjects/Keywords: Malaria parasite; Pfspdsyn inhibitors; Pfodc-adometdc inhibition; Polyamine biosynthesis; UCTD

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APA (6^th Edition):

author], [. (2009). Metabolomic analyses of the malaria parasite after inhibition of polyamine biosynthesis . (Masters Thesis). University of Pretoria. Retrieved from http://upetd.up.ac.za/thesis/available/etd-10072009-162558/

Chicago Manual of Style (16^th Edition):

author], [No. “Metabolomic analyses of the malaria parasite after inhibition of polyamine biosynthesis .” 2009. Masters Thesis, University of Pretoria. Accessed March 05, 2021. http://upetd.up.ac.za/thesis/available/etd-10072009-162558/.

MLA Handbook (7^th Edition):

author], [No. “Metabolomic analyses of the malaria parasite after inhibition of polyamine biosynthesis .” 2009. Web. 05 Mar 2021.

Vancouver:

author] [. Metabolomic analyses of the malaria parasite after inhibition of polyamine biosynthesis . [Internet] [Masters thesis]. University of Pretoria; 2009. [cited 2021 Mar 05]. Available from: http://upetd.up.ac.za/thesis/available/etd-10072009-162558/.

Council of Science Editors:

author] [. Metabolomic analyses of the malaria parasite after inhibition of polyamine biosynthesis . [Masters Thesis]. University of Pretoria; 2009. Available from: http://upetd.up.ac.za/thesis/available/etd-10072009-162558/

17. Müller, Kai-Sven. Synthese primärer Amine durch Hydroaminomethylierung und reduktive Aminierung.

Degree: 2004, Technische Universität Dortmund

URL: http://hdl.handle.net/2003/22185

► Es wird die Synthese primärer Amine durch Hydroaminomethylierung bzw. reduktive Aminierung mit Ammoniak und dessen Äquivalenten untersucht. Bei der rhodiumkatalysierten Hydroaminomethylierung von Monoolefinen und bei… (more)

Subjects/Keywords: Ammoniak; Harnstoff; Hydroaminomethylierung; Polyamine; Primäre Amine; Reduktive Aminierung; Tertiäre Amine; 540

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APA (6^th Edition):

Müller, K. (2004). Synthese primärer Amine durch Hydroaminomethylierung und reduktive Aminierung. (Thesis). Technische Universität Dortmund. Retrieved from http://hdl.handle.net/2003/22185

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Müller, Kai-Sven. “Synthese primärer Amine durch Hydroaminomethylierung und reduktive Aminierung.” 2004. Thesis, Technische Universität Dortmund. Accessed March 05, 2021. http://hdl.handle.net/2003/22185.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Müller, Kai-Sven. “Synthese primärer Amine durch Hydroaminomethylierung und reduktive Aminierung.” 2004. Web. 05 Mar 2021.

Vancouver:

Müller K. Synthese primärer Amine durch Hydroaminomethylierung und reduktive Aminierung. [Internet] [Thesis]. Technische Universität Dortmund; 2004. [cited 2021 Mar 05]. Available from: http://hdl.handle.net/2003/22185.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Müller K. Synthese primärer Amine durch Hydroaminomethylierung und reduktive Aminierung. [Thesis]. Technische Universität Dortmund; 2004. Available from: http://hdl.handle.net/2003/22185

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of California – Riverside

18. Ancheta, Allan Atienza. Putrescine-Mediated Changes in Mammalian Intracellular Polyamine Levels Increase Spermidine/Spermine-N1-Acetyltransferase Activity and Increase Gene Expression of Several Cell Cycle-Related Genes.

Degree: Biochemistry and Molecular Biology, 2010, University of California – Riverside

URL: http://www.escholarship.org/uc/item/67s421pv

► Polyamines are aliphatic polycations existing in all living organisms and are essential for life. Most organisms synthesize three types of polyamines: putrescine, spermidine, and spermine.… (more)

▼ Polyamines are aliphatic polycations existing in all living organisms and are essential for life. Most organisms synthesize three types of polyamines: putrescine, spermidine, and spermine. Putrescine is the product of the rate-limiting reaction of ornithine decarboxylase (ODC) and the amino acid ornithine. Spermidine and spermine are downstream metabolites sequentially derived from putrescine. In a recent landmark colon cancer chemopreventative clinical trial researchers found that combining alpha-difluoromethylornithine (DFMO), a selective ODC suicide inhibitor, and sulindac, a nonsteroidal anti-inflammatory drug, led to a 70% reduction of recurrence of all adenomas and a 90% reduction of recurrence of advanced and/or multiple adenomas over a 3-year treatment. This study shows that polyamine metabolic enzymes are attractive targets for possible chemotherapeutics. Our previous research has shown that stable ODC overexpression led to increased ODC activity, elevated intracellular putrescine levels with concomitant losses of spermidine and spermine, and increased spermidine/spermine-N1-acetyltransferase (SSAT) activity. Following a genome-wide array analysis on the effects of ODC overexpression in mammalian cell lines, several cell cycle-related genes were found to be upregulated. We investigated whether changes in polyamine levels, polyamine metabolism, and gene expression following stable ODC overexpression could be replicated following treatment with exogenously supplied putrescine. We observed that intracellular putrescine levels following treatment with exogenous putrescine accumulated to levels observed during stable ODC overexpression. These putrescine-mediated changes in intracellular putrescine pools also led to increased mRNA levels and activity of SSAT and to increased gene expression of ID1, c-Jun, and c-Fos in a time- and dose-dependent manner. We also determined that the observed increases in gene expression were mediated by the accumulation of intracellular putrescine independent from the activation of key, rate-limiting polyamine metabolic enzymes. Changes in intracellular polyamine pools, polyamine metabolism, and gene expression following the administration of exogenously supplied putrescine closely resembled the changes observed following stable ODC overexpression.

Subjects/Keywords: Chemistry, Biochemistry; Biology, Molecular; ID1; polyamine; putrescine; SSAT

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Ancheta, A. A. (2010). Putrescine-Mediated Changes in Mammalian Intracellular Polyamine Levels Increase Spermidine/Spermine-N1-Acetyltransferase Activity and Increase Gene Expression of Several Cell Cycle-Related Genes. (Thesis). University of California – Riverside. Retrieved from http://www.escholarship.org/uc/item/67s421pv

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Ancheta, Allan Atienza. “Putrescine-Mediated Changes in Mammalian Intracellular Polyamine Levels Increase Spermidine/Spermine-N1-Acetyltransferase Activity and Increase Gene Expression of Several Cell Cycle-Related Genes.” 2010. Thesis, University of California – Riverside. Accessed March 05, 2021. http://www.escholarship.org/uc/item/67s421pv.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Ancheta, Allan Atienza. “Putrescine-Mediated Changes in Mammalian Intracellular Polyamine Levels Increase Spermidine/Spermine-N1-Acetyltransferase Activity and Increase Gene Expression of Several Cell Cycle-Related Genes.” 2010. Web. 05 Mar 2021.

Vancouver:

Ancheta AA. Putrescine-Mediated Changes in Mammalian Intracellular Polyamine Levels Increase Spermidine/Spermine-N1-Acetyltransferase Activity and Increase Gene Expression of Several Cell Cycle-Related Genes. [Internet] [Thesis]. University of California – Riverside; 2010. [cited 2021 Mar 05]. Available from: http://www.escholarship.org/uc/item/67s421pv.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Ancheta AA. Putrescine-Mediated Changes in Mammalian Intracellular Polyamine Levels Increase Spermidine/Spermine-N1-Acetyltransferase Activity and Increase Gene Expression of Several Cell Cycle-Related Genes. [Thesis]. University of California – Riverside; 2010. Available from: http://www.escholarship.org/uc/item/67s421pv

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Texas A&M University

19. Kwon, Hyuk Jung. Amino acids, polyamines, and nitric oxide synthesis in the ovine conceptus.

Degree: MS, Physiology of Reproduction, 2005, Texas A&M University

URL: http://hdl.handle.net/1969.1/2257

► The objective of this study was to determine concentrations of amino acids and polyamines as well as nitric oxide (NO) and polyamine synthesis in the… (more)

▼ The objective of this study was to determine concentrations of amino acids and polyamines as well as nitric oxide (NO) and polyamine synthesis in the ovine conceptus (embryo/fetal and associated placental membrane). Ewes were hysterectomized on Days 30, 40, 60, 80, 100, 120, or 140 of gestation to obtain allantoic and amniotic fluids, intercotyledonary placenta, placentomes and uterine endometrium for the analyses. Alanine, citrulline plus glutamine accounted for about 80% of total α-amino acids in allantoic fluid during early gestation. Serine (16.5 mM) contributed about 60% of total α-amino acids in allantoic fluid on Day 140 of gestation. Maximal ornithine decarboxylase (ODC) and arginase activities and highest rates of polyamine and NO synthesis occured in all tissues on Day 40 of gestation. In ovine allantoic and amniotic fluids, polyamines were most abundant during early (Days 40-60) and late (Days 100-140) gestation, respectively. Activity of guanosine 5??-triphosphate-cyclohydrolase I (GTP-CH), and concentrations of NOS cofactors, tetrahydrobiopterin (BH4) and NADPH (nicotinamide adenine dinucleotide), peaked on Day 40 of gestation in placental and endometrial tissues. In these tissues, NO synthesis was positively correlated with total NOS activity, GTP-CH activity, and concentrations of BH4 and NADPH. The physiological significance of these changes was manifested by undernutrition-induced intrauterine growth retardation (IUGR). Maternal undernutrition (50% of National Research Council nutrient requirements) reduced concentrations of total α-amino acids in fetal plasma and fluids, and retarded fetal growth at both mid (Day 78) and late (Day 135) gestation. Concentrations of polyamines in fetal fluids were lower in underfed ewes than in control-fed ewes. Realimentation of underfed ewes between Days 78 and 135 of gestation increased concentrations of total α-amino acids and polyamines in fetal plasma and fluids, when compared with non-realimented ewes. Results of these studies demonstrate metabolic coordination among the several integrated pathways to enable high rates of polyamine and NO synthesis in the placenta and endometrium during early pregnancy. Collectively, our findings may have important implications for both IUGR and fetal origins of adult disease. Advisors/Committee Members: Wu, Guoyao (advisor), Spencer, Thomas E. (advisor), Meininger, Cynthia J. (committee member), Bazer, Fuller W. (committee member).

Subjects/Keywords: Amino acid; polyamine; NO; conceptus

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Kwon, H. J. (2005). Amino acids, polyamines, and nitric oxide synthesis in the ovine conceptus. (Masters Thesis). Texas A&M University. Retrieved from http://hdl.handle.net/1969.1/2257

Chicago Manual of Style (16^th Edition):

Kwon, Hyuk Jung. “Amino acids, polyamines, and nitric oxide synthesis in the ovine conceptus.” 2005. Masters Thesis, Texas A&M University. Accessed March 05, 2021. http://hdl.handle.net/1969.1/2257.

MLA Handbook (7^th Edition):

Kwon, Hyuk Jung. “Amino acids, polyamines, and nitric oxide synthesis in the ovine conceptus.” 2005. Web. 05 Mar 2021.

Vancouver:

Kwon HJ. Amino acids, polyamines, and nitric oxide synthesis in the ovine conceptus. [Internet] [Masters thesis]. Texas A&M University; 2005. [cited 2021 Mar 05]. Available from: http://hdl.handle.net/1969.1/2257.

Council of Science Editors:

Kwon HJ. Amino acids, polyamines, and nitric oxide synthesis in the ovine conceptus. [Masters Thesis]. Texas A&M University; 2005. Available from: http://hdl.handle.net/1969.1/2257

Queens University

20. Cushing, Alexander. Adsorption of Polyamine Chelated Copper Ions Onto Gangue Minerals and High Capacity Adsorbents .

Degree: Mining Engineering, 2014, Queens University

URL: http://hdl.handle.net/1974/8546

► The effluent quality from mining & processing operations is monitored to ensure that maximum allowable limits are not exceeded. Recently, copper concentration levels in the… (more)

▼ The effluent quality from mining & processing operations is monitored to ensure that maximum allowable limits are not exceeded. Recently, copper concentration levels in the effluent discharge flows of a copper and nickel mining company in Ontario have indicated increasing trends. A chemical particular to the problem is use of diethylenetriamine (DETA) in the process. Adsorption tests were conducted to investigate the ability of various adsorbents to remove and retain copper complexed with DETA and triethylenetetramine (TETA) in solutions. The tests were divided into two sections: gangue adsorbents (silica and pyrrhotite) and high capacity adsorbents (natural bentonite, peat, zeolite Y and zeolite ZSM-5). Pyrrhotite as a sulphide gangue had a greater adsorption capacity than silica for the concentration range studied. At 1 ppm initial concentration, over 80% of copper chelate was removed by minus 400 mesh pyrrhotite compared to 72% of the same size silica. Freundlich and Langmuir isotherm models of adsorption are applicable. However, the Langmuir adsorption isotherm was found to more closely represent the experimental data with a maximum adsorption capacity of 129.9 μg/g for copper complexed with DETA on pyrrhotite. For the high capacity adsorbents, natural bentonite, zeolite Y and peat each worked well at removing the copper chelates. Zeolite Y had the highest capacity for copper chelates and a maximum adsorption capacity of 55.9 mg/g. Freundlich and Langmuir adsorption isotherm models were studied with the Langmuir isotherm model more closely representing the experimental data. iii Studies were also conducted on the effect of temperature. This led to a thermodynamic analysis of adsorption and estimation of activation energies. The standard free energies estimated for adsorption of copper chelated on adsorbents studied were nearly always negative, typically varying from around -2 kJ/mol to -7 kJ/mol with increasing temperature. The activation energy was found to be highest for the natural bentonite system suggesting a strong adsorption (e.g. 40.5 kJ/mol for CuTETA). Desorption experiments on the peat indicated very poor reversal for the process, confirming that the adsorption of copper chelates on high capacity adsorption was indeed very strong. Settling experiments indicated copper chelates were highly effective as coagulants on bentonite.

Subjects/Keywords: Adsorption ; Mineral Processing ; Tailings ; Copper ; Mining ; Flotation ; Chelate ; Polyamine

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Cushing, A. (2014). Adsorption of Polyamine Chelated Copper Ions Onto Gangue Minerals and High Capacity Adsorbents . (Thesis). Queens University. Retrieved from http://hdl.handle.net/1974/8546

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Cushing, Alexander. “Adsorption of Polyamine Chelated Copper Ions Onto Gangue Minerals and High Capacity Adsorbents .” 2014. Thesis, Queens University. Accessed March 05, 2021. http://hdl.handle.net/1974/8546.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Cushing, Alexander. “Adsorption of Polyamine Chelated Copper Ions Onto Gangue Minerals and High Capacity Adsorbents .” 2014. Web. 05 Mar 2021.

Vancouver:

Cushing A. Adsorption of Polyamine Chelated Copper Ions Onto Gangue Minerals and High Capacity Adsorbents . [Internet] [Thesis]. Queens University; 2014. [cited 2021 Mar 05]. Available from: http://hdl.handle.net/1974/8546.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Cushing A. Adsorption of Polyamine Chelated Copper Ions Onto Gangue Minerals and High Capacity Adsorbents . [Thesis]. Queens University; 2014. Available from: http://hdl.handle.net/1974/8546

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Pretoria

21. Reeksting, S.B. (Shaun Bernard). Metabolomic analyses of the malaria parasite after inhibition of polyamine biosynthesis.

Degree: Biochemistry, 2009, University of Pretoria

URL: http://hdl.handle.net/2263/28508

► Malaria, a disease transmitted by female mosquitoes, has plagued the world for many centuries. The disease is associated with high mortality rates, severe poverty, and… (more)

▼ Malaria, a disease transmitted by female mosquitoes, has plagued the world for many centuries. The disease is associated with high mortality rates, severe poverty, and economic burden. These are factors which hamper effective eradication of the disease. Drug resistant forms of the parasite have caused increasing concerns and questioned the longevity of current effective antimalarials. Efforts are therefore aimed at the identification and exploitation of essential parasite proteins as potential drug targets. The polyamine pathway of Plasmodium falciparum is an exploitable pathway which contains two distinct, chemically validated drug targets; a bifunctional PfAdoMetDC-ODC protein and PfSpdSyn. These enzymes ensure intricate regulation of polyamine production and the pathway contains various distinctive features which could be selectively targetable from the mammalian counterpart pathways. However, inhibition of polyamine production through the use of specific enzyme inhibitors has revealed various compensatory responses that negate the efficacy of these inhibitors. An account is given of the metabolomic fluctuations in the parasite during inhibition of polyamine biosynthesis. From co-inhibited P. falciparum extracts, it could be demonstrated that the characteristic growth-arrest coincided with the depletion in spermidine, the metabolic product of PfSpdSyn. The co-inhibition strategy therefore emphasised the importance of spermidine biosynthesis by PfSpdSyn. Moreover, adenosyl-related metabolite levels were not disrupted during polyamine depletion, supporting the notion that these metabolites are intricately recycled within the parasites. The identified metabolic compensatory mechanisms have further potential for exploitation, and can strategically be combined with polyamine biosynthesis inhibition to ensure parasitic attenuation. In addition, several novel inhibitors were previously computationally identified, based on a dynamic receptor-based pharmacophore model of PfSpdSyn. The in vitro inhibiting activity of these compounds was determined against PfSpdSyn. Results from the in vitro experiments supported the in silico predictions, and emphasized the supportive role of pharmacophore modelling has for the identification of novel inhibitors. The research contributed in understanding parasitic polyamine metabolite regulation, and will aid in the future optimization of therapeutic strategies, aimed at exploitation of the polyamine pathway as a potential antimalarial drug target. Copyright Advisors/Committee Members: Birkholtz, Lyn-Marie (advisor), Louw, Abraham Izak (advisor).

Subjects/Keywords: Malaria parasite; Pfspdsyn inhibitors; Pfodc-adometdc inhibition; Polyamine biosynthesis; UCTD

Record Details Similar Records Cite « Share

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Reeksting, S. B. (. (2009). Metabolomic analyses of the malaria parasite after inhibition of polyamine biosynthesis. (Masters Thesis). University of Pretoria. Retrieved from http://hdl.handle.net/2263/28508

Chicago Manual of Style (16^th Edition):

Reeksting, S B (Shaun. “Metabolomic analyses of the malaria parasite after inhibition of polyamine biosynthesis.” 2009. Masters Thesis, University of Pretoria. Accessed March 05, 2021. http://hdl.handle.net/2263/28508.

MLA Handbook (7^th Edition):

Reeksting, S B (Shaun. “Metabolomic analyses of the malaria parasite after inhibition of polyamine biosynthesis.” 2009. Web. 05 Mar 2021.

Vancouver:

Reeksting SB(. Metabolomic analyses of the malaria parasite after inhibition of polyamine biosynthesis. [Internet] [Masters thesis]. University of Pretoria; 2009. [cited 2021 Mar 05]. Available from: http://hdl.handle.net/2263/28508.

Council of Science Editors:

Reeksting SB(. Metabolomic analyses of the malaria parasite after inhibition of polyamine biosynthesis. [Masters Thesis]. University of Pretoria; 2009. Available from: http://hdl.handle.net/2263/28508

University of Colorado

22. VanFosson, Alex Philip. Molecular Simulations Studies of the Effect of Ligand Architecture on DNA Binding.

Degree: MS, Chemical & Biochemical Engineering, 2013, University of Colorado

URL: https://scholar.colorado.edu/chbe_gradetds/49

► In this paper we use atomistic molecular dynamics simulations to study the structural reasons underlying the DNA binding efficacy of several polyamine-aminoglycoside compounds. We… (more)

Subjects/Keywords: aminoglycoside; atomistic; dilysine; molecular simulations; polyamine; spermine; Biochemistry; Molecular Biology

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

VanFosson, A. P. (2013). Molecular Simulations Studies of the Effect of Ligand Architecture on DNA Binding. (Masters Thesis). University of Colorado. Retrieved from https://scholar.colorado.edu/chbe_gradetds/49

Chicago Manual of Style (16^th Edition):

VanFosson, Alex Philip. “Molecular Simulations Studies of the Effect of Ligand Architecture on DNA Binding.” 2013. Masters Thesis, University of Colorado. Accessed March 05, 2021. https://scholar.colorado.edu/chbe_gradetds/49.

MLA Handbook (7^th Edition):

VanFosson, Alex Philip. “Molecular Simulations Studies of the Effect of Ligand Architecture on DNA Binding.” 2013. Web. 05 Mar 2021.

Vancouver:

VanFosson AP. Molecular Simulations Studies of the Effect of Ligand Architecture on DNA Binding. [Internet] [Masters thesis]. University of Colorado; 2013. [cited 2021 Mar 05]. Available from: https://scholar.colorado.edu/chbe_gradetds/49.

Council of Science Editors:

VanFosson AP. Molecular Simulations Studies of the Effect of Ligand Architecture on DNA Binding. [Masters Thesis]. University of Colorado; 2013. Available from: https://scholar.colorado.edu/chbe_gradetds/49

23. Baumbach, Jordan Lillian. Functional characterization of a fungal polyamine oxidase FvPO1.

Degree: 2018, Iowa State University

URL: https://lib.dr.iastate.edu/etd/16913

► Soybean is an important source of protein and oil for both human and animal nutrition. The United States is the world’s leading soybean producing nation.… (more)

▼ Soybean is an important source of protein and oil for both human and animal nutrition. The United States is the world’s leading soybean producing nation. In 2016, the U.S. soybean production was valued over 40.9 billion dollars. Sudden death syndrome (SDS) of soybean is ranked as one of the top ten yield limiting disease of soybean and is caused by the pathogen Fusarium virguliforme. Fusarium virguliforme is a soil borne fungal pathogen, which infects soybean roots resulting in both root sot symptoms and foliar chlorosis and necrosis. To date there is no known single gene resistance to SDS, the resistance that has been found is complex and heavily influenced by the environment. To gain a better understanding of the role of fungal genes in SDS development, RNA-sequencing of F. virguliforme infected soybean roots was conducted. Many F. virguliforme genes involved in cell wall degradation as well as phytoalexin detoxification were up-regulated during infection. Interestingly, the fungus also increases expression of a fungal polyamine oxidase gene FvPO1. Little is known about the role of fungal polyamine oxidases. Therefore, knockout mutants and complimented mutants for FvPO1 gene were created. Mutant studies confirmed the FvPO1 enzymes polyamine oxidase activity. The Δfvpo1 mutant did not have altered growth, sporulation or soybean infection phenotypes. Transgenic soybean plants expressing FvPO1 did not have altered defense response to either infection with F. virguliforme or to infection with the bacterial pathogen Pseudomonas syringae pv glycinea. Comparisons of the transcriptomes of soybeans inoculated with Δfvpo1 and the complimented Δfvpo1::FvPO1 mutant showed that the presence of FvPO1 does not alter the expression of soybean genes following F. virguliforme infection. Interestingly, three fungal genes related to the metabolism of the secondary metabolite phenylacetic acid were upregulated in the Δfvpo1 mutant fungus during infection. Phenylacetic acid has been suggested to play a role in induced systemic resistance in plants. When F. virguliforme was grown on media containing phenylacetic acid fungal growth was reduced. Δfvpo1 mutants do not have altered growth phenotype when compared to the wild type on the phenylacetic acid media. FvPO1 has increased expression during F. virguliforme infection of soybean roots but is not necessary for F. virguliforme to infect soybean or cause typical symptoms. FvPO1 is increased with addition of the polyamines spermine and spermidine to the media. Therefore, the increased expression of FvPO1 following infection may be due to the presence of plant polyamines and the pathogen may use plant polyamines as an additional nutrient source during infection. Based on transcriptomics study, FvPO1 expression appears to regulate the phenylacetic acid metabolism pathway.

Subjects/Keywords: Fungal; Fusarium virguliforme; Polyamine oxidase; Soybean; Sudden death syndrome; Genetics

10 more images…

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Baumbach, J. L. (2018). Functional characterization of a fungal polyamine oxidase FvPO1. (Thesis). Iowa State University. Retrieved from https://lib.dr.iastate.edu/etd/16913

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Baumbach, Jordan Lillian. “Functional characterization of a fungal polyamine oxidase FvPO1.” 2018. Thesis, Iowa State University. Accessed March 05, 2021. https://lib.dr.iastate.edu/etd/16913.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Baumbach, Jordan Lillian. “Functional characterization of a fungal polyamine oxidase FvPO1.” 2018. Web. 05 Mar 2021.

Vancouver:

Baumbach JL. Functional characterization of a fungal polyamine oxidase FvPO1. [Internet] [Thesis]. Iowa State University; 2018. [cited 2021 Mar 05]. Available from: https://lib.dr.iastate.edu/etd/16913.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Baumbach JL. Functional characterization of a fungal polyamine oxidase FvPO1. [Thesis]. Iowa State University; 2018. Available from: https://lib.dr.iastate.edu/etd/16913

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Waterloo

24. Waduwara-Jayabahu, Chammika Ishari. Significance of Methylthioadenosine Metabolism to Plant Growth and Development.

Degree: 2012, University of Waterloo

URL: http://hdl.handle.net/10012/6517

► Arabidopsis thaliana contains two genes annotated as methylthioadenosine nucleosidases (MTN): MTN1, At4g38800 and MTN2, At4g34840. This enzyme activity hydrolyzes the methylthioadenosine (MTA) produced by nicotianamine… (more)

▼ Arabidopsis thaliana contains two genes annotated as methylthioadenosine nucleosidases (MTN): MTN1, At4g38800 and MTN2, At4g34840. This enzyme activity hydrolyzes the methylthioadenosine (MTA) produced by nicotianamine (NA), polyamine (PA), and ethylene biosynthesis to methylthioribose (MTR) within the Yang cycle. Comprehensive analysis of the mtn1-1mtn2-1 mutant line with 14 % residual MTN activity revealed a complex phenotype that includes male and female infertility and abnormal vascular development. Based on metabolite profiling, mtn1-1mtn2-1 has a reduced NA content, altered PA profiles with higher putrescine (Put) and lower spermidine (Spd) and spermine (Spm) levels, disrupted metal ion profiles, and abnormal auxin distribution. The modeling of Arabidopsis PA synthases developed by comparison with the crystal structures of human Spd and spermine synthases complexed with MTA suggests that Arabidopsis PA synthases are product inhibited by MTA. Thus, these pleiotropic mutant phenotypes possibly are the result of one metabolite directly inhibiting numerous pathways. By creating and analyzing a series of mutants and transgenic lines with moderate levels of MTN activity the complex phenotype of mtn1-1mtn2-1 was dissected in order to determine the fundamental trait associated with MTN deficiency. Two double mutants were identified by crossing single T-DNA mutants, and an artificial micro RNA (amiRNA) line was generated by transforming mtn1-1 with amiRNA specific to MTN2. The T-DNA double mutants, mtn1 4mtn2-1, and mtn1-1mtn2-5 had 98 % and 28 % MTN activity, respectively, whereas the amiRNA line has 16 % MTN activity. The growth, development, and metabolite analysis of these mutants revealed that their delayed bolting, correlated with an increased number of leaves, was the common trait observed across all lines. Xylem proliferation defects and increased number of vascular bundles per unit area were shared in all lines except mtn1 4mtn2-1. Based on these results, auxin distribution is proposed as the key target of the accumulated MTA that results from MTN deficiency. The infertility related to MTN-deficiency was restored by supplying 100 µM of Spd to the mtn1-1mtn2-1 seedlings over 14 days. The data presented in this thesis reveals two potential links that work synergistically to recover fertility in this mtn1-1mtn2-1 line. Based on a detailed analysis of the female gynoecia morphology, transcript, hormone and metabolite profiles, it is proposed that the Spd partially reverses the mutant phenotypes through the recovery of auxin distribution and /or vascular development. Interestingly, the Spd effect seems to be transgenerational: they give rise to plants that are genotypically mtn1-1mtn2-1 but phenotypically WT over generations. Taken together, all of the results suggest that MTN-deficient mutants provide the potential for unraveling the molecular mechanism associated with nicotianamine, polyamines, auxin, and vascular development with respect to enhancing the efficiency of nutrient use and yields in plants.

Subjects/Keywords: methylthioadenosine nucleosidases; methylthioadenosine; polyamine; Yang cycle; vascular development; auxin

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Waduwara-Jayabahu, C. I. (2012). Significance of Methylthioadenosine Metabolism to Plant Growth and Development. (Thesis). University of Waterloo. Retrieved from http://hdl.handle.net/10012/6517

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Waduwara-Jayabahu, Chammika Ishari. “Significance of Methylthioadenosine Metabolism to Plant Growth and Development.” 2012. Thesis, University of Waterloo. Accessed March 05, 2021. http://hdl.handle.net/10012/6517.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Waduwara-Jayabahu, Chammika Ishari. “Significance of Methylthioadenosine Metabolism to Plant Growth and Development.” 2012. Web. 05 Mar 2021.

Vancouver:

Waduwara-Jayabahu CI. Significance of Methylthioadenosine Metabolism to Plant Growth and Development. [Internet] [Thesis]. University of Waterloo; 2012. [cited 2021 Mar 05]. Available from: http://hdl.handle.net/10012/6517.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Waduwara-Jayabahu CI. Significance of Methylthioadenosine Metabolism to Plant Growth and Development. [Thesis]. University of Waterloo; 2012. Available from: http://hdl.handle.net/10012/6517

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Louisiana State University

25. Guan, Ying. The Role of Polyamines in Osmotic Stress Tolerance in Gulf Killifish Fundulus Grandis.

Degree: PhD, 2013, Louisiana State University

URL: etd-06272013-193138 ; https://digitalcommons.lsu.edu/gradschool_dissertations/1123

► Polyamines are a diverse class of aliphatic molecules that are highly polycationic at physiological intracellular pH. Polyamine levels are regulated by the coordinated actions of… (more)

▼ Polyamines are a diverse class of aliphatic molecules that are highly polycationic at physiological intracellular pH. Polyamine levels are regulated by the coordinated actions of arginase (Arg), ornithine decarboxylase (Odc), and polyamine oxidase (Pao). Although little is known of their functions in fish, polyamines have been implicated in diverse physiological processes, including RNA transcription, cell growth, cell proliferation, and cell apoptosis. The main objective of this study was to describe the transcription and enzymatic activities of key enzymes for polyamine metabolism, to measure polyamine levels, and to assess putative roles of polyamines in the gills of the Gulf killifish (Fundulus grandis) during hypoosmotic challenge. In addition, the influence of irreversible inhibition of Odc by alpha-DL-difluoromethylornithine (DFMO) was assessed in the gills. Furthermore, the transcription and enzymatic activities of Arg, Odc and Pao was assessed in other tissues such as intestine and liver during hypoosmoitc challenges. Adult F. grandis were reared in 5 ppt and acutely transferred to 5, 2, 1, 0.5, and 0.1 ppt water, and gills were sampled at 6 h, 1 d, 3 d, and 7 d post-transfer. Results showed that arg II and odc mRNA levels were highly upregulated in the gills during the first few days post-transfer to hypoosmotic water. Hypoosmotic exposure also produced increases in gill Odc activity and in the concentrations of putrescine, spermidine, and spermine. DFMO application inhibited Odc activity and reduced polyamine levels after hypoosmotic exposure (0.1 ppt). Although gill putrescine levels remained elevated throughout the first 7 d post transfer to 0.1 ppt, the concentrations of spermidine and spermine decreased over time. The ratio of putrescine level over the sum levels of spermidine and spermine increased after 0.1 ppt exposure at 1 d and beyond. Concomitant with freshwater acclimation, an increase in Pao activity suggested that polyamine catabolism was upregulated in the gills. The phenotype of mitochondrion-rich cells (MRCs) in the gill epithelium shifted from a seawater type to a freshwater type following transfer to 0.1 ppt water in correlation with the increase in mRNA levels of arg II and odc in MRCs. In addition, the isolated opercular epithelium pretreated with spermidine had a lower active Cl- secretion rate and membrane conductance following symmetrical hypotonic exposure. Transcription and enzymatic activities of Arg II, Odc, and Pao were upregulated in the intestine and liver during hypoosmotic exposure, suggesting that polyamine levels are regulated in multiple tissues of the killifish. The putative roles of polyamines include inducing cell apoptosis by increasing caspase-3 activity, stimulating cell proliferation by increasing the levels of c-fos and c-myc mRNA levels, and inducing cell swelling via the modulation of Cl- secretion in the gills following hypoosmotic challenges. In summary, fish gill and intestine of killifish transferred to fresh water underwent dramatic physiological and…

Subjects/Keywords: ornithine decarboxylase; arginase; osmoregulation; polyamine; killifish; gill epithelium; gill remodeling

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APA (6^th Edition):

Guan, Y. (2013). The Role of Polyamines in Osmotic Stress Tolerance in Gulf Killifish Fundulus Grandis. (Doctoral Dissertation). Louisiana State University. Retrieved from etd-06272013-193138 ; https://digitalcommons.lsu.edu/gradschool_dissertations/1123

Chicago Manual of Style (16^th Edition):

Guan, Ying. “The Role of Polyamines in Osmotic Stress Tolerance in Gulf Killifish Fundulus Grandis.” 2013. Doctoral Dissertation, Louisiana State University. Accessed March 05, 2021. etd-06272013-193138 ; https://digitalcommons.lsu.edu/gradschool_dissertations/1123.

MLA Handbook (7^th Edition):

Guan, Ying. “The Role of Polyamines in Osmotic Stress Tolerance in Gulf Killifish Fundulus Grandis.” 2013. Web. 05 Mar 2021.

Vancouver:

Guan Y. The Role of Polyamines in Osmotic Stress Tolerance in Gulf Killifish Fundulus Grandis. [Internet] [Doctoral dissertation]. Louisiana State University; 2013. [cited 2021 Mar 05]. Available from: etd-06272013-193138 ; https://digitalcommons.lsu.edu/gradschool_dissertations/1123.

Council of Science Editors:

Guan Y. The Role of Polyamines in Osmotic Stress Tolerance in Gulf Killifish Fundulus Grandis. [Doctoral Dissertation]. Louisiana State University; 2013. Available from: etd-06272013-193138 ; https://digitalcommons.lsu.edu/gradschool_dissertations/1123

26. Leblond, Pierre. Etude des mécanismes d'action de nouvelles molécules utilisées seules ou en association avec les radiations ionisantes dans les cancers pédiatriques : Study of the mechanisms of action of new drugs used as single drugs or in combination with ionizing radiation in pediatric cancers.

Degree: Docteur es, Cancérologie ; radiothérapie, 2013, Université Lille II – Droit et Santé

URL: http://www.theses.fr/2013LIL2S030

►

Les progrès considérables effectués durant les trente dernières années permettent de guérir actuellement plus de 75% des enfants atteints d’un cancer. Néanmoins, certains types de… (more)

▼

Les progrès considérables effectués durant les trente dernières années permettent de guérir actuellement plus de 75% des enfants atteints d’un cancer. Néanmoins, certains types de tumeur, comme les gliomes de haut grade et les neuroblastomes métastatiques des enfants de plus de un an, gardent un pronostic particulièrement sombre. De nouvelles stratégies thérapeutiques doivent donc être développées dans ces indications. Dans cette optique, nous avons étudié les effets de deux nouvelles molécules ciblées, le cilengitide, inhibiteur des intégrines αvβ3 et αvβ5, et le F14512, inhibiteur de la topoisomérase II dont la délivrance est vectorisée via le système de transport des polyamines, sur des modèles précliniques de gliomes pédiatriques et de neuroblastomes.Nous avons montré pour la première fois l’existence d’une expression variable d’αvβ3 et αvβ5 dans nos modèles de lignées cellulaires pédiatriques, peu modifiée par les radiations ionisantes. Le traitement par cilengitide a entraîné une inhibition dose-dépendante de la croissance des cellules exprimant αvβ3, liée à un rapide détachement cellulaire et à une sensibilité à l’anoïkis. Cette inhibition de croissance et le détachement cellulaire n’étaient pas corrélés au niveau d’expression des intégrines αvβ3 et αvβ5. Néanmoins, nos travaux semblent montrer que la cible principale du cilengitide est l’intégrine αvβ3. Le traitement par radiations ionisantes n’a pas modifié le détachement induit par le cilengitide dans nos modèles.Nous avons également mis en évidence une activité du système de transport des polyamines dans nos modèles, permettant ainsi une incorporation active du F14512 dans nos cellules de neuroblastomes. Nous avons montré une supériorité de la cytotoxicité du F14512 par rapport à l’étoposide in vitro, et son effet antitumoral a été démontré sur un modèle in vivo. Ces résultats, ainsi que l’effet globalement synergique de l’association du F14512 avec les sels de platine, sont de solides arguments pour poursuivre le développement de cette molécule, en phase clinique chez les patients atteints d’un neuroblastome. Par ailleurs, d’autres investigations pourraient être envisagées dans d’autres types tumoraux dans lesquels l’étoposide occupe une place importante. L’effet radiosensibilisant du F14512 pourrait dans ce cadre ouvrir des perspectives dans la prise en charge des médulloblastomes.[...]

Despite the progress made during the past thirty years leading to cure about 75% of children with cancer, the prognosis of high-grade gliomas (HGG) in children and metastatic neuroblastoma remains poor. The aim of the thesis was to study in vitro the mechanisms of action of a novel inhibitor of αvβ3 and αvβ5 integrins, so-called cilengitide , and of a new topoisomerase II inhibitor targeting the polyamine transport system (PTS), so-called F14512, used as a single drug or in combination therapy in neuroblastoma and pediatric gliomas cell lines. Our panel of cell lines included three pediatric HGG, 2 low-grade pediatric glioma and four neuroblastoma cell lines as well as…

Advisors/Committee Members: Cappelaere-Lansiaux, Amélie (thesis director).

Subjects/Keywords: F14512; Inhibiteur de la topoisomérase; F14512; Polyamine transport system

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Leblond, P. (2013). Etude des mécanismes d'action de nouvelles molécules utilisées seules ou en association avec les radiations ionisantes dans les cancers pédiatriques : Study of the mechanisms of action of new drugs used as single drugs or in combination with ionizing radiation in pediatric cancers. (Doctoral Dissertation). Université Lille II – Droit et Santé. Retrieved from http://www.theses.fr/2013LIL2S030

Chicago Manual of Style (16^th Edition):

Leblond, Pierre. “Etude des mécanismes d'action de nouvelles molécules utilisées seules ou en association avec les radiations ionisantes dans les cancers pédiatriques : Study of the mechanisms of action of new drugs used as single drugs or in combination with ionizing radiation in pediatric cancers.” 2013. Doctoral Dissertation, Université Lille II – Droit et Santé. Accessed March 05, 2021. http://www.theses.fr/2013LIL2S030.

MLA Handbook (7^th Edition):

Leblond, Pierre. “Etude des mécanismes d'action de nouvelles molécules utilisées seules ou en association avec les radiations ionisantes dans les cancers pédiatriques : Study of the mechanisms of action of new drugs used as single drugs or in combination with ionizing radiation in pediatric cancers.” 2013. Web. 05 Mar 2021.

Vancouver:

Leblond P. Etude des mécanismes d'action de nouvelles molécules utilisées seules ou en association avec les radiations ionisantes dans les cancers pédiatriques : Study of the mechanisms of action of new drugs used as single drugs or in combination with ionizing radiation in pediatric cancers. [Internet] [Doctoral dissertation]. Université Lille II – Droit et Santé 2013. [cited 2021 Mar 05]. Available from: http://www.theses.fr/2013LIL2S030.

Council of Science Editors:

Leblond P. Etude des mécanismes d'action de nouvelles molécules utilisées seules ou en association avec les radiations ionisantes dans les cancers pédiatriques : Study of the mechanisms of action of new drugs used as single drugs or in combination with ionizing radiation in pediatric cancers. [Doctoral Dissertation]. Université Lille II – Droit et Santé 2013. Available from: http://www.theses.fr/2013LIL2S030

University of Canterbury

27. Thornley, Paul Andrew. The Synthesis and Characterisation of a Novel Polyamine-Terpyridine Ligand and Related Complexes.

Degree: MS, Chemistry, 2009, University of Canterbury

URL: http://dx.doi.org/10.26021/7453

► This project was aimed at synthesising, characterising and examining the properties of the novel polyamine ligand 4’-{2’”-(12-Amino-2,6,9-triazadodecyl)-phenyl}-2,2’:6’,2”-terpyridine and its related complexes. The ligand would be… (more)

Subjects/Keywords: Ligands; Polyamine; Transition metals; Terpyridine

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APA (6^th Edition):

Thornley, P. A. (2009). The Synthesis and Characterisation of a Novel Polyamine-Terpyridine Ligand and Related Complexes. (Masters Thesis). University of Canterbury. Retrieved from http://dx.doi.org/10.26021/7453

Chicago Manual of Style (16^th Edition):

Thornley, Paul Andrew. “The Synthesis and Characterisation of a Novel Polyamine-Terpyridine Ligand and Related Complexes.” 2009. Masters Thesis, University of Canterbury. Accessed March 05, 2021. http://dx.doi.org/10.26021/7453.

MLA Handbook (7^th Edition):

Thornley, Paul Andrew. “The Synthesis and Characterisation of a Novel Polyamine-Terpyridine Ligand and Related Complexes.” 2009. Web. 05 Mar 2021.

Vancouver:

Thornley PA. The Synthesis and Characterisation of a Novel Polyamine-Terpyridine Ligand and Related Complexes. [Internet] [Masters thesis]. University of Canterbury; 2009. [cited 2021 Mar 05]. Available from: http://dx.doi.org/10.26021/7453.

Council of Science Editors:

Thornley PA. The Synthesis and Characterisation of a Novel Polyamine-Terpyridine Ligand and Related Complexes. [Masters Thesis]. University of Canterbury; 2009. Available from: http://dx.doi.org/10.26021/7453

University of Montana

28. Gleason, William J. USE AND MACRO-MOLECULAR STRUCTURE OF SILICA POLYAMINE COMPOSITES.

Degree: PhD, 2007, University of Montana

URL: https://scholarworks.umt.edu/etd/1241

► The use and macro-molecular structure of silica polyamine composites was examined through a series of experiments. Precious metals were removed from both prepared and industrial… (more)

Subjects/Keywords: composites; gold; polyamine; FTIR; seperation

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APA (6^th Edition):

Gleason, W. J. (2007). USE AND MACRO-MOLECULAR STRUCTURE OF SILICA POLYAMINE COMPOSITES. (Doctoral Dissertation). University of Montana. Retrieved from https://scholarworks.umt.edu/etd/1241

Chicago Manual of Style (16^th Edition):

Gleason, William J. “USE AND MACRO-MOLECULAR STRUCTURE OF SILICA POLYAMINE COMPOSITES.” 2007. Doctoral Dissertation, University of Montana. Accessed March 05, 2021. https://scholarworks.umt.edu/etd/1241.

MLA Handbook (7^th Edition):

Gleason, William J. “USE AND MACRO-MOLECULAR STRUCTURE OF SILICA POLYAMINE COMPOSITES.” 2007. Web. 05 Mar 2021.

Vancouver:

Gleason WJ. USE AND MACRO-MOLECULAR STRUCTURE OF SILICA POLYAMINE COMPOSITES. [Internet] [Doctoral dissertation]. University of Montana; 2007. [cited 2021 Mar 05]. Available from: https://scholarworks.umt.edu/etd/1241.

Council of Science Editors:

Gleason WJ. USE AND MACRO-MOLECULAR STRUCTURE OF SILICA POLYAMINE COMPOSITES. [Doctoral Dissertation]. University of Montana; 2007. Available from: https://scholarworks.umt.edu/etd/1241

University of Pennsylvania

29. Hai, Yang. Structure and Function of Metallohydrolases in the Arginase-Deacetylase Family.

Degree: 2016, University of Pennsylvania

URL: https://repository.upenn.edu/edissertations/1753

► Arginases and deacetylases are metallohydrolases that catalyze two distinct chemical transformations. The arginases catalyze the hydrolysis of the guanidinium group of arginine by using a… (more)

▼ Arginases and deacetylases are metallohydrolases that catalyze two distinct chemical transformations. The arginases catalyze the hydrolysis of the guanidinium group of arginine by using a hydroxide ion bridging the binuclear manganese cluster (Mn2+A-Mn2+B) for nucleophilic attack. The deacetylases catalyze the hydrolysis of amide bonds by using a mononuclear Zn2+-ion activated water molecule as the nucleophile. Despite the diverse functions, metallohydrolases of the arginase-deacetylase superfamily share the same characteristic α/β hydrolase core fold and a conserved metal binding site (the Mn2+B site in arginase corresponds to the catalytic Zn2+ site in deacetylase) which is essential for catalysis in both enzymes. We report crystal structure of formiminoglutamase from the parasitic protozoan Trypanosoma cruzi and confirm that formiminoglutamase is a Mn2+-requiring hydrolase that belongs to the arginase-deacetylase superfamily. We also report the crystal structure of an arginase-like protein from Trypanosoma brucei (TbARG) with unknown function. Although its biological role remains enigmatic, the evolutionarily more conserved Mn2+B site can be readily restored in TbARG through side-directed mutagenesis. We also report crystal structure of an arginase from the parasite Schistosoma mansoni (SmARG). Structural comparison of SmARG complexed with second-generation arginase inhibitors (α,α-disubstituted boronic acid inhibitors) with another parasitic arginase from Leishmania mexicana and human arginases reveal interesting differences in the binding modes of the additional α-substituents. Reversible lysine acetylation rivals phosphorylation in the regulation of protein structure and function, and inhibition of the “eraser” histone deacetylase (HDAC) is a validated approach for cancer chemotherapy. HDAC6, the sole HDAC that harbors a full duplication of catalytic domain (CD1 and CD2), is a cytosolic lysine deacetylase known to deacetylate α-tubulin, heat-shock protein 90, etc. Here, we report HDAC6 structures that provide new insights about mechanism, catalysis, and inhibitor binding. In light of biochemical studies, we reveal a “gate constriction” mechanism responsible for the strict substrate specificity of CD1 versus broad substrate specificity of CD2. Analysis of other isozymes indicates that the closest relative HDAC10 contains an alternative gatekeeper that favors catalysis with acetylpolyamines. Indeed, we provide structural evidence that HDAC10 is the long-sought mammalian N8-acetylspermidine deacetylase whose identity has remained elusive for 30 years.

Subjects/Keywords: Arginase; Crystallography; Histone deacetylase; Metallohydrolase; polyamine deacetylase; Biochemistry

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APA (6^th Edition):

Hai, Y. (2016). Structure and Function of Metallohydrolases in the Arginase-Deacetylase Family. (Thesis). University of Pennsylvania. Retrieved from https://repository.upenn.edu/edissertations/1753

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Hai, Yang. “Structure and Function of Metallohydrolases in the Arginase-Deacetylase Family.” 2016. Thesis, University of Pennsylvania. Accessed March 05, 2021. https://repository.upenn.edu/edissertations/1753.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Hai, Yang. “Structure and Function of Metallohydrolases in the Arginase-Deacetylase Family.” 2016. Web. 05 Mar 2021.

Vancouver:

Hai Y. Structure and Function of Metallohydrolases in the Arginase-Deacetylase Family. [Internet] [Thesis]. University of Pennsylvania; 2016. [cited 2021 Mar 05]. Available from: https://repository.upenn.edu/edissertations/1753.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Hai Y. Structure and Function of Metallohydrolases in the Arginase-Deacetylase Family. [Thesis]. University of Pennsylvania; 2016. Available from: https://repository.upenn.edu/edissertations/1753

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Bowling Green State University

30. Patel, Jigarkumar J. The Role of Polyamine Uptake Transporters on Growth and Development of Arabidopsis Thaliana.

Degree: PhD, Biological Sciences, 2015, Bowling Green State University

URL: http://rave.ohiolink.edu/etdc/view?acc_num=bgsu1429280174

► Transgenic manipulation of polyamine levels has provided compelling evidence that polyamines enable plants to respond to environmental cues by activation of stress and developmental pathways.… (more)

Subjects/Keywords: Biology; Plant Biology; Polyamines; Polyamine Transporters; Putrescine biosynthesis

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APA (6^th Edition):

Patel, J. J. (2015). The Role of Polyamine Uptake Transporters on Growth and Development of Arabidopsis Thaliana. (Doctoral Dissertation). Bowling Green State University. Retrieved from http://rave.ohiolink.edu/etdc/view?acc_num=bgsu1429280174

Chicago Manual of Style (16^th Edition):

Patel, Jigarkumar J. “The Role of Polyamine Uptake Transporters on Growth and Development of Arabidopsis Thaliana.” 2015. Doctoral Dissertation, Bowling Green State University. Accessed March 05, 2021. http://rave.ohiolink.edu/etdc/view?acc_num=bgsu1429280174.

MLA Handbook (7^th Edition):

Patel, Jigarkumar J. “The Role of Polyamine Uptake Transporters on Growth and Development of Arabidopsis Thaliana.” 2015. Web. 05 Mar 2021.

Vancouver:

Patel JJ. The Role of Polyamine Uptake Transporters on Growth and Development of Arabidopsis Thaliana. [Internet] [Doctoral dissertation]. Bowling Green State University; 2015. [cited 2021 Mar 05]. Available from: http://rave.ohiolink.edu/etdc/view?acc_num=bgsu1429280174.

Council of Science Editors:

Patel JJ. The Role of Polyamine Uptake Transporters on Growth and Development of Arabidopsis Thaliana. [Doctoral Dissertation]. Bowling Green State University; 2015. Available from: http://rave.ohiolink.edu/etdc/view?acc_num=bgsu1429280174

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Open Access Theses and Dissertations