subject:(Point of care)

1. Wang, Jingjing. Microfluidics based Molecular Assays for Point-of-Care Diagnostics.

Degree: PhD, Biomedical Engineering, 2014, Brown University

URL: https://repository.library.brown.edu/studio/item/bdr:386180/

► Microfluidics based diagnostic systems are believed to have great competency for the ever-demanding healthcare in resource limited settings. These miniaturized devices have promise for detecting… (more)

▼ Microfluidics based diagnostic systems are believed to have great competency for the ever-demanding healthcare in resource limited settings. These miniaturized devices have promise for detecting biological targets with high quality and low cost. A compact point- of-care device would expand their diagnostic potential. This dissertation presents some of the scientific insights in development of microfluidic platforms for separation, amplification and detection of biomolecules ranging from proteins to viral RNA in influenza patient samples. The research examined critical aspects of isolation and separation of biomolecules using a mobile magnetic bead based platform without any use of external flow system. New models were constructed based on the kinetics and thermodynamics aspects of assays. A novel microfluidic SMART (Simple Method for Amplifying RNA Targets) assay was developed for the detection of H1 seasonal, H1 pandemic and H3 influenza A vRNA subtypes from clinical specimens. The sensitivity and selectivity of the assay was performed using a cohort of 116 patient samples. The SMART correctly detected influenza virus in 99% of the samples and accurately provided subtyping information (95.7% concordance to RT-PCR test result). A new frontier of rapid diagnostics for infectious diseases is highlighted by the threat of increasing drug resistance due to mutations in critical genes. Many of these mutations can be characterized via Single Nucleotide Polymorphisms (SNP’s). This dissertation also presents a new assay for detecting K103N drug resistance mutation in HIV-1 through direct enzymatic ligation of two oligonucleotides on a RNA template, and in a single reaction mixture. Following this, an original method for the fast detection, and microfluidic separation and quantification of ligated product is developed. Nucleic acid sample isolation can be further integrated in the platform, and we are currently developing this capability in a microfluidic chip. In conclusion, microfluidic devices were designed and developed to study fundamental properties of proteins, RNA and DNA in creating new diagnostic paradigms. Advisors/Committee Members: Tripathi, Anubhav (Director), Tang, Jay (Director), Darling, Eric (Reader), Palmore, G. Tayhas (Reader), Opal, Steven (Reader), Cohen, Seth (Reader), Tripathi, Anubhav (Director), Tang, Jay (Director), Darling, Eric (Reader), Palmore, G. Tayhas (Reader), Opal, Steven (Reader), Cohen, Seth (Reader).

Subjects/Keywords: point-of-care

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APA (6^th Edition):

Wang, J. (2014). Microfluidics based Molecular Assays for Point-of-Care Diagnostics. (Doctoral Dissertation). Brown University. Retrieved from https://repository.library.brown.edu/studio/item/bdr:386180/

Chicago Manual of Style (16^th Edition):

Wang, Jingjing. “Microfluidics based Molecular Assays for Point-of-Care Diagnostics.” 2014. Doctoral Dissertation, Brown University. Accessed February 25, 2021. https://repository.library.brown.edu/studio/item/bdr:386180/.

MLA Handbook (7^th Edition):

Wang, Jingjing. “Microfluidics based Molecular Assays for Point-of-Care Diagnostics.” 2014. Web. 25 Feb 2021.

Vancouver:

Wang J. Microfluidics based Molecular Assays for Point-of-Care Diagnostics. [Internet] [Doctoral dissertation]. Brown University; 2014. [cited 2021 Feb 25]. Available from: https://repository.library.brown.edu/studio/item/bdr:386180/.

Council of Science Editors:

Wang J. Microfluidics based Molecular Assays for Point-of-Care Diagnostics. [Doctoral Dissertation]. Brown University; 2014. Available from: https://repository.library.brown.edu/studio/item/bdr:386180/

University of Illinois – Urbana-Champaign

2. Sun, Fu. Multiplexed detection of infectious pathogens using on-chip loop-mediated isothermal amplification and a smartphone.

Degree: MS, Electrical & Computer Engr, 2017, University of Illinois – Urbana-Champaign

URL: http://hdl.handle.net/2142/99239

► New tools are needed to enable rapid detection, identification, and reporting of infectious pathogens in a wide variety of point-of-care applications that impact human and… (more)

Subjects/Keywords: Detection; Pathogens; Point-of-care

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APA (6^th Edition):

Sun, F. (2017). Multiplexed detection of infectious pathogens using on-chip loop-mediated isothermal amplification and a smartphone. (Thesis). University of Illinois – Urbana-Champaign. Retrieved from http://hdl.handle.net/2142/99239

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Sun, Fu. “Multiplexed detection of infectious pathogens using on-chip loop-mediated isothermal amplification and a smartphone.” 2017. Thesis, University of Illinois – Urbana-Champaign. Accessed February 25, 2021. http://hdl.handle.net/2142/99239.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Sun, Fu. “Multiplexed detection of infectious pathogens using on-chip loop-mediated isothermal amplification and a smartphone.” 2017. Web. 25 Feb 2021.

Vancouver:

Sun F. Multiplexed detection of infectious pathogens using on-chip loop-mediated isothermal amplification and a smartphone. [Internet] [Thesis]. University of Illinois – Urbana-Champaign; 2017. [cited 2021 Feb 25]. Available from: http://hdl.handle.net/2142/99239.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Sun F. Multiplexed detection of infectious pathogens using on-chip loop-mediated isothermal amplification and a smartphone. [Thesis]. University of Illinois – Urbana-Champaign; 2017. Available from: http://hdl.handle.net/2142/99239

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Queens University

3. Li, Xu Liang. Modeling of pCO2 Point-of-Care Devices .

Degree: Chemical Engineering, 2014, Queens University

URL: http://hdl.handle.net/1974/8622

► A dynamic model is developed and presented that predicts the voltage response for a Severinghaus electrode-based point-of-care pCO2 sensor. Eight partial differential equations are derived… (more)

▼ A dynamic model is developed and presented that predicts the voltage response for a Severinghaus electrode-based point-of-care pCO2 sensor. Eight partial differential equations are derived to describe the diffusion and reaction phenomena in the sensor. The model is able to predict the potential response versus time behaviour from different CO2 concentrations in the calibration fluid and control fluids. The two most influential and uncertain parameters in the model are determined to be the forward rate constant for benzoquinone consumption at the gold surface ( k_(f_Au ) ), and the partition coefficient for CO2 between the membrane and the electrolyte (κ_(〖CO〗_(2_m ) )). These parameters were adjusted heuristically to obtain a good fit (within 2 mV) between the dynamic voltage response data and the model predictions during a critical 4 second period. The model predictions are sufficient for design sensitivity studies, however an improved fit might be possible using a formal least-squares parameter estimation approach, or if additional parameters were estimated. Several design parameters are varied to study the influence of the electrolyte concentration and the sensor geometry on the voltage response. The most influential design parameter studied is the amount of water present in the electrolyte during sensor operation. This can be affected by the amount of water evaporated during manufacturing and storage, and by the amount of water present when the sensor “wets up” again during operation. The amount of water picked up by the sensor in turn is affected by design parameters such as component/membrane dimensions and thicknesses. The initial buffer concentration in the electrolyte is the second most influential parameter. The resulting model can be used to perform “what if” analyses in order to understand the impact of design decisions on the sensor performance, and to potentially improve the sensor from performance and manufacturing cost perspectives.

Subjects/Keywords: Modeling ; Point of Care

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APA (6^th Edition):

Li, X. L. (2014). Modeling of pCO2 Point-of-Care Devices . (Thesis). Queens University. Retrieved from http://hdl.handle.net/1974/8622

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Li, Xu Liang. “Modeling of pCO2 Point-of-Care Devices .” 2014. Thesis, Queens University. Accessed February 25, 2021. http://hdl.handle.net/1974/8622.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Li, Xu Liang. “Modeling of pCO2 Point-of-Care Devices .” 2014. Web. 25 Feb 2021.

Vancouver:

Li XL. Modeling of pCO2 Point-of-Care Devices . [Internet] [Thesis]. Queens University; 2014. [cited 2021 Feb 25]. Available from: http://hdl.handle.net/1974/8622.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Li XL. Modeling of pCO2 Point-of-Care Devices . [Thesis]. Queens University; 2014. Available from: http://hdl.handle.net/1974/8622

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Tulane University

4. Tulman, David. Quantitative evaluation and optimization of video-rate structured illumination microscopy (VR-SIM) for clinical applications in point-of-procedure tissue assessment.

Degree: 2018, Tulane University

URL: https://digitallibrary.tulane.edu/islandora/object/tulane:87837

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[email protected]

This dissertation is rooted in clinical pathology research, and the main character is addressing limitations in current pathology evaluation workflows. Diagnostic procedures for cancer… (more)

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[email protected]

This dissertation is rooted in clinical pathology research, and the main character is addressing limitations in current pathology evaluation workflows. Diagnostic procedures for cancer are typically conducted via core needle biopsy procedures; however, tissue sampling limitations often result in a low yield of samples containing cancer – there are no reliable intraoperative methods to determine if the “lesion is in the needle”. If biopsy procedures result in a diagnosis of cancer, surgical removal of the tumor is often the frontline curative therapy for many cancers. Importantly, histologic evaluation following the whole resected organ is necessary to determine the presence of residual cancer, yet current methods do not allow efficient determination of tumor removal completeness intraoperatively. To address limitations of current histopathology methods, what is critically needed is a point-of-procedure fresh tissue evaluation system that facilitates 1) rapid on-site imaging and evaluation, 2) less destruction, and 3) more complete assessment of tumor content in fresh specimens. A novel microscopy system using video-rate structured illumination (VR-SIM), has been developed with the intent of rapid, point-of-procedure histological screening of intact biopsy and whole surgical specimens. VR-SIM leverages widefield imaging, rapid acting fluorescent stains, and optical sectioning to provide high contrast digital images of tissue with histological relevance. The method is to replicate the standard approach as closely as possible, but replace the physical section with an optically sectioned digital image. The overall goal of this work is to perform technological and methodological refinements necessary to translate VR-SIM as a clinical tool for histologic evaluation of fresh tissue in diagnostic procedures, biobanking, and tumor margin assessment. This project will lay the groundwork for quantitative evaluation of VR-SIM as a clinical tool – with the goal of leading toward industrial design of a VR-SIM as a medical device for hospital use. Developing a new framework for integration of high throughput microscopy into the clinical and research workflow, as well as developing new methods for quantification and evaluation of clinical effectiveness these tools will be presented and discussed in the context of patient outcomes and economic impact.

1

David Tulman

Advisors/Committee Members: Brown, Jonathon Q. (Thesis advisor), School of Science & Engineering Biomedical Engineering (Degree granting institution).

Subjects/Keywords: digital pathology; microscopy; point-of-care diagnostics

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APA (6^th Edition):

Tulman, D. (2018). Quantitative evaluation and optimization of video-rate structured illumination microscopy (VR-SIM) for clinical applications in point-of-procedure tissue assessment. (Thesis). Tulane University. Retrieved from https://digitallibrary.tulane.edu/islandora/object/tulane:87837

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Tulman, David. “Quantitative evaluation and optimization of video-rate structured illumination microscopy (VR-SIM) for clinical applications in point-of-procedure tissue assessment.” 2018. Thesis, Tulane University. Accessed February 25, 2021. https://digitallibrary.tulane.edu/islandora/object/tulane:87837.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Tulman, David. “Quantitative evaluation and optimization of video-rate structured illumination microscopy (VR-SIM) for clinical applications in point-of-procedure tissue assessment.” 2018. Web. 25 Feb 2021.

Vancouver:

Tulman D. Quantitative evaluation and optimization of video-rate structured illumination microscopy (VR-SIM) for clinical applications in point-of-procedure tissue assessment. [Internet] [Thesis]. Tulane University; 2018. [cited 2021 Feb 25]. Available from: https://digitallibrary.tulane.edu/islandora/object/tulane:87837.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Tulman D. Quantitative evaluation and optimization of video-rate structured illumination microscopy (VR-SIM) for clinical applications in point-of-procedure tissue assessment. [Thesis]. Tulane University; 2018. Available from: https://digitallibrary.tulane.edu/islandora/object/tulane:87837

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Cornell University

5. Gumus, Abdurrahman. Bioelectronic Systems In Studying Tissue Engineering, Real-Time Biophysical Monitoring Of Birds And Point-Of-Care Diagnostics.

Degree: PhD, Electrical Engineering, 2015, Cornell University

URL: http://hdl.handle.net/1813/39295

► The field of bioelectronics started about 18th century with the frog experiments of Luigi Galvani by moving the detached leg of frog with the application… (more)

▼ The field of bioelectronics started about 18th century with the frog experiments of Luigi Galvani by moving the detached leg of frog with the application of a small voltage. Today there are variety of bioelectronic devices available in many different areas such as pacemakers, continuous glucose sensors, implantable brain tissue interfaces, that show how far we have gone since the Galvani experiments. This dissertation introduces bioelectronic systems for different research areas such as tissue engineering, biophysical monitoring of birds and point-of-care diagnostics. First, we have introduced a device depends on organic bioelectronics, a growing research field that integrates organic electronic materials with biological systems, and used it for tissue engineering purposes. We have developed a planar device that contains a conducting polymer stripe and achieves a continuum of microenvironments for cell growth under the influence of an applied bias. Marked differences are observed in the migration behaviors of bovine aortic endothelial cells (EC) as a function of location along the polymer stripe, and 3-fold variation is achieved in EC migration speed and directional persistence time. A gradient in adsorbed fibronectin indicates that a spatial variation in cell adhesion is at play. We have used our device to modulate the cell adhesion and changed cell density gradients of normal and cancerous cell lines by inducing electrically which can be used as a tool for the study of cell-cell interactions. Next, we have developed a real-time in vivo uric acid biosensor system, Labon-a-Bird, for biophysical monitoring of birds. The metabolism of birds is finely tuned to their activities and environments, and thus research on avian systems can play an important role in understanding organismal responses to environmental changes and ecological investigations. After characterization of the sensor system, we demonstrated the autonomous operation of the system by collecting in vivo extracellular uric acid measurements on a domestic chicken. We then show how the device can be used to monitor, in real time, the effects of short-term flight and rest cycles on the uric acid levels of pigeons. In addition, we demonstrate that our device has the ability to measure uric acid level increase in homing pigeons while they fly freely to back home. Successful application of the sensor in migratory birds could open up a new way of studying birds in flight which would lead to a better understanding of the ecology and biology of avian movements. Finally, we have presented a Cholera-Detect system for point-of-care detection of Vibrio Cholerae which is a comma-shaped, gram negative bacterium and the cause of an acute diarrhoeal disease in humans called "Cholera". Even though up to 80% of the cases can be successfully treated with oral rehydration salts, around 100,000 - 120,000 of the cases come to an end as deaths. This indicates that early and rapid detection of the cholera is necessary to prevent spread of disease, increase the efficiency of… Advisors/Committee Members: Erickson, David (chair), Manohar, Rajit (committee member), Winkler, David Ward (committee member), Baeumner, Antje J (committee member).

Subjects/Keywords: Bioelectronics; Biophysical monitoring; Point-of-care diagnostics

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APA (6^th Edition):

Gumus, A. (2015). Bioelectronic Systems In Studying Tissue Engineering, Real-Time Biophysical Monitoring Of Birds And Point-Of-Care Diagnostics. (Doctoral Dissertation). Cornell University. Retrieved from http://hdl.handle.net/1813/39295

Chicago Manual of Style (16^th Edition):

Gumus, Abdurrahman. “Bioelectronic Systems In Studying Tissue Engineering, Real-Time Biophysical Monitoring Of Birds And Point-Of-Care Diagnostics.” 2015. Doctoral Dissertation, Cornell University. Accessed February 25, 2021. http://hdl.handle.net/1813/39295.

MLA Handbook (7^th Edition):

Gumus, Abdurrahman. “Bioelectronic Systems In Studying Tissue Engineering, Real-Time Biophysical Monitoring Of Birds And Point-Of-Care Diagnostics.” 2015. Web. 25 Feb 2021.

Vancouver:

Gumus A. Bioelectronic Systems In Studying Tissue Engineering, Real-Time Biophysical Monitoring Of Birds And Point-Of-Care Diagnostics. [Internet] [Doctoral dissertation]. Cornell University; 2015. [cited 2021 Feb 25]. Available from: http://hdl.handle.net/1813/39295.

Council of Science Editors:

Gumus A. Bioelectronic Systems In Studying Tissue Engineering, Real-Time Biophysical Monitoring Of Birds And Point-Of-Care Diagnostics. [Doctoral Dissertation]. Cornell University; 2015. Available from: http://hdl.handle.net/1813/39295

Vanderbilt University

6. -6623-3163. Nanocalorimeter Platform for Point-of-Care Medical Applications.

Degree: PhD, Biomedical Engineering, 2020, Vanderbilt University

URL: http://hdl.handle.net/1803/10116

► Point-of-care (POC) diagnostics is an important field of biomedical research, delivering rapid results in nonlaboratory settings. Advances in microfabrication have given rise to calorimeters with… (more)

Subjects/Keywords: Nanocalorimeter; TELISA; Biosensor; Point-of-care

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APA (6^th Edition):

-6623-3163. (2020). Nanocalorimeter Platform for Point-of-Care Medical Applications. (Doctoral Dissertation). Vanderbilt University. Retrieved from http://hdl.handle.net/1803/10116

Note: this citation may be lacking information needed for this citation format:
Author name may be incomplete

Chicago Manual of Style (16^th Edition):

-6623-3163. “Nanocalorimeter Platform for Point-of-Care Medical Applications.” 2020. Doctoral Dissertation, Vanderbilt University. Accessed February 25, 2021. http://hdl.handle.net/1803/10116.

Note: this citation may be lacking information needed for this citation format:
Author name may be incomplete

MLA Handbook (7^th Edition):

-6623-3163. “Nanocalorimeter Platform for Point-of-Care Medical Applications.” 2020. Web. 25 Feb 2021.

Note: this citation may be lacking information needed for this citation format:
Author name may be incomplete

Vancouver:

-6623-3163. Nanocalorimeter Platform for Point-of-Care Medical Applications. [Internet] [Doctoral dissertation]. Vanderbilt University; 2020. [cited 2021 Feb 25]. Available from: http://hdl.handle.net/1803/10116.

Note: this citation may be lacking information needed for this citation format:
Author name may be incomplete

Council of Science Editors:

-6623-3163. Nanocalorimeter Platform for Point-of-Care Medical Applications. [Doctoral Dissertation]. Vanderbilt University; 2020. Available from: http://hdl.handle.net/1803/10116

Note: this citation may be lacking information needed for this citation format:
Author name may be incomplete

7. Brannelly, Niamh. The development of a point of care device for measuring blood ammonia.

Degree: PhD, 2017, University of the West of England, Bristol

URL: https://uwe-repository.worktribe.com/output/900674 ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.704121

► Ammonia is produced in the body during the metabolism of amino acids. In the liver, it is converted to urea via the urea cycle and… (more)

▼ Ammonia is produced in the body during the metabolism of amino acids. In the liver, it is converted to urea via the urea cycle and excreted by the kidneys as urine. Normal levels are between 11 to 50 µM, whereas a blood ammonia level of approximately 100 µM indicates pathology. Elevated blood ammonia is associated with a number of pathological conditions including liver and kidney dysfunction. Conditions such as these can affect brain function and can be fatal. Current blood ammonia analysis requires a laboratory blood test. Few, if any of the techniques used are suitable for point of care (POC) testing. The development of a reliable and simple method for blood ammonia determination is essential for clinical diagnosis and management of patient progress in order to prevent further debilitating illnesses developing, and extending life. This is particularly critical in many disorders such as hyperammonaemia of the newborn, inborn errors of metabolism including urea cycle defects, organic acidaemias, hyperinsulinism/hyperammonaemia, liver disease and other cause of hyperammonaemic encephalopathy. This thesis investigates the development of an electrochemical sensor for the measurement of ammonia in blood. Polyaniline has a known affinity for ammonia which operates on the deprotonation of the polyaniline backbone forming an ammonium ion. In this work, polyaniline nanoparticles were fabricated and inkjet-printed onto silver screen printed electrodes. The sensors were then incorporated into devices containing a gas-permeable membrane, which facilitated the measurement of gaseous ammonia from a liquid sample (blood) using electrochemical impedance spectroscopy. The combination of impedance spectroscopy with a gas-permeable membrane allowed the measurement of gaseous ammonia from solution. The ammonia device developed possessed refinements to enhance its sensitivity and included careful optimisation of other aspects of the measurement. For example, an air purge through the device gas chamber was employed to remove matrix interferences from the sensor and improve the specificity to ammonia. The pH of the sample to be analysed was modified in order to increase the mass of ammonia in solution, thus lowering the limit of detection (LOD) of the device. Finally, assay timings were optimised in order to increase the impedimetric response of ammonia. These optimisations resulted in the effective detection of ammonia in a liquid sample down to the lowest clinically relevant levels found in blood. The devices displayed an impedimetric baseline intra- and inter-variability of 25 and 6.9%, respectively for n = 15 over a period of 160 s. A calculated limit of LOD of 12 µM was achieved for human serum measurements. A coefficient of determination of 0.9984, slope of 0.0046 and an intercept of 1.1534 was obtained in human serum across the linear range of 25 to 200 μM ammonia (n = 3). The device was validated against a commercial spectrophotometric assay which resulted in excellent correlation (0.9699, p < 0.0001) with a slope of 1.4472 and…

Subjects/Keywords: 612.1; ammonia; point of care; polyaniline

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Brannelly, N. (2017). The development of a point of care device for measuring blood ammonia. (Doctoral Dissertation). University of the West of England, Bristol. Retrieved from https://uwe-repository.worktribe.com/output/900674 ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.704121

Chicago Manual of Style (16^th Edition):

Brannelly, Niamh. “The development of a point of care device for measuring blood ammonia.” 2017. Doctoral Dissertation, University of the West of England, Bristol. Accessed February 25, 2021. https://uwe-repository.worktribe.com/output/900674 ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.704121.

MLA Handbook (7^th Edition):

Brannelly, Niamh. “The development of a point of care device for measuring blood ammonia.” 2017. Web. 25 Feb 2021.

Vancouver:

Brannelly N. The development of a point of care device for measuring blood ammonia. [Internet] [Doctoral dissertation]. University of the West of England, Bristol; 2017. [cited 2021 Feb 25]. Available from: https://uwe-repository.worktribe.com/output/900674 ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.704121.

Council of Science Editors:

Brannelly N. The development of a point of care device for measuring blood ammonia. [Doctoral Dissertation]. University of the West of England, Bristol; 2017. Available from: https://uwe-repository.worktribe.com/output/900674 ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.704121

University of Toronto

8. De Haan, Kevin David. Point-of-Care Detection System for Multiplexed Bead-Based Assays.

Degree: 2017, University of Toronto

URL: http://hdl.handle.net/1807/79136

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We demonstrate an optical detection system which can be used to measure multiplexed bead based immunoassays. The assays can detect the presence of multiple analytes… (more)

Subjects/Keywords: Bead Immunoassay; Point-of-care; 0544

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APA (6^th Edition):

De Haan, K. D. (2017). Point-of-Care Detection System for Multiplexed Bead-Based Assays. (Masters Thesis). University of Toronto. Retrieved from http://hdl.handle.net/1807/79136

Chicago Manual of Style (16^th Edition):

De Haan, Kevin David. “Point-of-Care Detection System for Multiplexed Bead-Based Assays.” 2017. Masters Thesis, University of Toronto. Accessed February 25, 2021. http://hdl.handle.net/1807/79136.

MLA Handbook (7^th Edition):

De Haan, Kevin David. “Point-of-Care Detection System for Multiplexed Bead-Based Assays.” 2017. Web. 25 Feb 2021.

Vancouver:

De Haan KD. Point-of-Care Detection System for Multiplexed Bead-Based Assays. [Internet] [Masters thesis]. University of Toronto; 2017. [cited 2021 Feb 25]. Available from: http://hdl.handle.net/1807/79136.

Council of Science Editors:

De Haan KD. Point-of-Care Detection System for Multiplexed Bead-Based Assays. [Masters Thesis]. University of Toronto; 2017. Available from: http://hdl.handle.net/1807/79136

University of Toronto

9. Pham, Ngoc Minh. Blood Filtration for Multiplexed Point-of-care Diagnostic Devices.

Degree: 2012, University of Toronto

URL: http://hdl.handle.net/1807/33674

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In the developing world, there are large populations suffering from infectious diseases, many of whom are located in remote regions. With the rapid growth in… (more)

Subjects/Keywords: microfluidics, point of care; blood filtration; 0548

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APA (6^th Edition):

Pham, N. M. (2012). Blood Filtration for Multiplexed Point-of-care Diagnostic Devices. (Masters Thesis). University of Toronto. Retrieved from http://hdl.handle.net/1807/33674

Chicago Manual of Style (16^th Edition):

Pham, Ngoc Minh. “Blood Filtration for Multiplexed Point-of-care Diagnostic Devices.” 2012. Masters Thesis, University of Toronto. Accessed February 25, 2021. http://hdl.handle.net/1807/33674.

MLA Handbook (7^th Edition):

Pham, Ngoc Minh. “Blood Filtration for Multiplexed Point-of-care Diagnostic Devices.” 2012. Web. 25 Feb 2021.

Vancouver:

Pham NM. Blood Filtration for Multiplexed Point-of-care Diagnostic Devices. [Internet] [Masters thesis]. University of Toronto; 2012. [cited 2021 Feb 25]. Available from: http://hdl.handle.net/1807/33674.

Council of Science Editors:

Pham NM. Blood Filtration for Multiplexed Point-of-care Diagnostic Devices. [Masters Thesis]. University of Toronto; 2012. Available from: http://hdl.handle.net/1807/33674

University of Toronto

10. Rackus, Darius George. Tools for Point-of-Care Digital Microfluidics.

Degree: PhD, 2017, University of Toronto

URL: http://hdl.handle.net/1807/93027

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Digital microfluidics (DMF) is a liquid handling technology for the manipulation of discrete droplets on a generic array of hydrophobic insulated electrodes. In DMF, protocols… (more)

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Digital microfluidics (DMF) is a liquid handling technology for the manipulation of discrete droplets on a generic array of hydrophobic insulated electrodes. In DMF, protocols are generated through software and are independent of the microfluidic architecture. DMF can be used to miniaturize and automate routine assays. Of interest are miniaturized medical diagnostic assays that could be performed in an automated fashion and can take place close to the point of need. This thesis describes the development of several tools for the realization of this description. The feasibility of performing clinical assays with DMF outside of the laboratory was demonstrated for the first time. DMF immunoassays for anti-measles IgG and anti-rubella IgG were performed in Kakuma, a refugee camp in northwestern Kenya. Custom-built open-source control systems and low-cost inkjet-printed cartridges were used to perform up to four chemiluminescent immunoassays simultaneously. 144 samples were tested and the clinical sensitivities and specificities of the DMF immunoassays were shown to be good (>80%). Having taken DMF out of the laboratory, new tools and detection methods were developed. First, nanostructured microelectrodes (NMEs) for electrochemical analysis were integrated with a DMF device. This was then applied to an immunoassay for anti-rubella IgG. Performance comparable to chemiluminescent methods was achieved. A second tool developed was a method entitled pre-concentration by liquid intake by paper (P-CLIP). Functionalized magnetic microparticles are captured from large (50-300 ÂľL) volumes of liquid and are resuspended in a much smaller volume (1-2 ÂľL). P-CLIP was used with an immunoassay for Pf lactate dehydrogenase and shown to improve signals up to 30-fold. The last tool for DMF clinical assays was the integration of low-cost commercial electrodes. Established methods for integrating sensing electrodes with DMF are costly and have low fabrication throughput. A plastic-based DMF top plate that can receive commercial biosensors and screen-printed electrodes was demonstrated. The sum of the innovations described in this thesis suggest that DMF is a viable platform for point-of-care analysis. Advancement to the tools already described will enable DMF to be used for analysis of a wide range of non-invasive samples such as finger-prick blood samples and saliva samples.

2018-12-19 00:00:00

Advisors/Committee Members: Wheeler, Aaron R, Chemistry.

Subjects/Keywords: diagnostics; microfluidics; point-of-care; 0486

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Rackus, D. G. (2017). Tools for Point-of-Care Digital Microfluidics. (Doctoral Dissertation). University of Toronto. Retrieved from http://hdl.handle.net/1807/93027

Chicago Manual of Style (16^th Edition):

Rackus, Darius George. “Tools for Point-of-Care Digital Microfluidics.” 2017. Doctoral Dissertation, University of Toronto. Accessed February 25, 2021. http://hdl.handle.net/1807/93027.

MLA Handbook (7^th Edition):

Rackus, Darius George. “Tools for Point-of-Care Digital Microfluidics.” 2017. Web. 25 Feb 2021.

Vancouver:

Rackus DG. Tools for Point-of-Care Digital Microfluidics. [Internet] [Doctoral dissertation]. University of Toronto; 2017. [cited 2021 Feb 25]. Available from: http://hdl.handle.net/1807/93027.

Council of Science Editors:

Rackus DG. Tools for Point-of-Care Digital Microfluidics. [Doctoral Dissertation]. University of Toronto; 2017. Available from: http://hdl.handle.net/1807/93027

Georgia Tech

11. Mannino, Robert G. A noninvasive, image-based smartphone app for diagnosing anemia.

Degree: PhD, Biomedical Engineering (Joint GT/Emory Department), 2018, Georgia Tech

URL: http://hdl.handle.net/1853/61131

► Smartphone-based telehealth is steadily transforming the delivery of medical care worldwide, moving diagnosis of disease from the clinic to the home to potentially anywhere in… (more)

▼ Smartphone-based telehealth is steadily transforming the delivery of medical care worldwide, moving diagnosis of disease from the clinic to the home to potentially anywhere in the globe. Smartphone images alone have recently been used by physicians to remotely diagnose a myriad of diseases. However, smartphone telehealth approaches have yet to non-invasively replace blood-based testing, which remains a major cornerstone of disease diagnosis in modern medicine. While the addition of specialized smartphone attachments and supplemental calibration tools may enable point-of-care diagnosis and analysis of tissue and bodily fluid samples, the additional burden of blood and/or tissue sample collections combined with the additional cost and inconvenience associated with this equipment, prevents worldwide use of these potentially disruptive approaches. Therefore, a smartphone-based system, requiring nothing other than the smartphones native technology and capable of non-invasively replacing blood-based diagnostics, would transform the very nature of telehealth and the delivery of healthcare worldwide. Towards that end, I specifically focused on anemia, a potentially life-threatening disorder characterized by low blood hemoglobin (Hgb) levels that affects approximately 2 billion people worldwide. Despite the high prevalence of anemia, all existing diagnostic approaches to measure Hgb require specialized equipment and represent tradeoffs between invasiveness, accuracy, infrastructure needs, and expense. Aside from being cost-prohibitive, the necessary invasive blood sampling to measure Hgb levels causes discomfort and trauma in younger pediatric patients. By examining clinical pallor, a common symptom of anemia, I developed a methodology that quantitatively analyzes patient-sourced photos using smartphone-based algorithms to enable a noninvasive, accurate, and accessible anemia diagnostic. Here, a patient simply takes a picture of their fingernail beds using their smartphone, and the image analysis algorithm analyzes color data and image metadata to measure the corresponding Hgb level. By quantifying clinical pallor, this system non-invasively measures Hgb levels to within a clinically significant and well accepted margin of error (±2.6 g/dL) of the gold standard Hgb measurement tool with a sensitivity and specificity of 0.90 and 0.82, respectively, of predicting anemia (defined as Hgb < 11.0g/dL) in 100 pediatric patients at Children’s Healthcare of Atlanta with anemia of any etiology mixed with healthy subjects. This algorithm has been implemented into a smartphone app that is capable of outperforming trained hematologists in physical examination-based Hgb measurement. Overall, this technology has the capability to change the treatment paradigm for anemia as patients no longer need to visit a clinic to monitor their hemoglobin. In this thesis, I discuss the development of this image analysis algorithm and the implementation of the algorithm into a smartphone app. Advisors/Committee Members: Lam, Wilbur A. (advisor), Boudreaux, Jeanne (committee member), Clifford, Gari D. (committee member), Cooper, Lee A. D. (committee member), Qiu, Peng (committee member).

Subjects/Keywords: Anemia; Point-of-care diagnostics; mHealth

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Mannino, R. G. (2018). A noninvasive, image-based smartphone app for diagnosing anemia. (Doctoral Dissertation). Georgia Tech. Retrieved from http://hdl.handle.net/1853/61131

Chicago Manual of Style (16^th Edition):

Mannino, Robert G. “A noninvasive, image-based smartphone app for diagnosing anemia.” 2018. Doctoral Dissertation, Georgia Tech. Accessed February 25, 2021. http://hdl.handle.net/1853/61131.

MLA Handbook (7^th Edition):

Mannino, Robert G. “A noninvasive, image-based smartphone app for diagnosing anemia.” 2018. Web. 25 Feb 2021.

Vancouver:

Mannino RG. A noninvasive, image-based smartphone app for diagnosing anemia. [Internet] [Doctoral dissertation]. Georgia Tech; 2018. [cited 2021 Feb 25]. Available from: http://hdl.handle.net/1853/61131.

Council of Science Editors:

Mannino RG. A noninvasive, image-based smartphone app for diagnosing anemia. [Doctoral Dissertation]. Georgia Tech; 2018. Available from: http://hdl.handle.net/1853/61131

University of New South Wales

12. Natoli, Lisa. Health service implications of the introduction of STI point-of-care testing in Australia.

Degree: Kirby Institute, 2015, University of New South Wales

URL: http://handle.unsw.edu.au/1959.4/56876 ; https://unsworks.unsw.edu.au/fapi/datastream/unsworks:41829/SOURCE02?view=true

► Chlamydia trachomatis (CT) and Neisseria gonorrhoea (NG) are curable sexually transmissible infections (STIs) which represent a significant public health burden, particularly in young Australia Aboriginal… (more)

▼ Chlamydia trachomatis (CT) and Neisseria gonorrhoea (NG) are curable sexually transmissible infections (STIs) which represent a significant public health burden, particularly in young Australia Aboriginal people in remote communities. Prompt testing and treatment is fundamental to STI control yet distance to urban laboratories is a significant barrier. CT/NG point-of-care (POC) testing offers an ideal solution, but until recently had been unavailable. This thesis aims to identify settings where POC testing could be beneficial, benefits and barriers to implementation and health service staff acceptability.The thesis was based on two sets of qualitative interviews. The first occurred with 18 Australian key informants with remote, sexual health and laboratory expertise, and generated three discrete studies. The second occurred with 16 trained nurses and Aboriginal health workers (AHWs) from the first 12 remote primary care services internationally to use GeneXpert CT/NG POC testing and resulted in study four. Study one focused on settings where the technology would have greatest benefit, with remote Aboriginal communities most commonly identified, as well as juvenile justice and outreach services to highly mobile or marginalised populations. In study two, informants identified the benefits of POC use for clinical practice including improved management of STIs- more timely and targeted treatment, earlier commencement of partner notification, and reduced effort associated with client recall, but noted it will result in changes to the STI management pathway, and policy and clinical guidelines may need to be altered. Study three focused on the public health implications; with the key perceived benefit being STI control, and barriers including the potential to negatively impact on disease notification and NG antibiotic sensitivity surveillance. In study four, most nurses and AHWs found the test easy to use and useful, and reported improved management of STIs consistent with the key informant’s perceived benefits. In conclusion, this thesis has provided information to inform implementation of CT/NG POC testing, including selection of appropriate settings, and the need to review clinical guidelines and establish systems to avoid adverse impact on public health surveillance. Importantly the research demonstrated the new technology was highly acceptable to staff working in remote primary care. Advisors/Committee Members: Guy, Rebecca, Kirby Institute, Faculty of Medicine, UNSW, Maher, Lisa, Kirby Institute, Faculty of Medicine, UNSW, Shephard, mark, Flinders University, Anderson, David, Burnet Institute.

Subjects/Keywords: Aboriginal communities; STI; Point of care

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APA (6^th Edition):

Natoli, L. (2015). Health service implications of the introduction of STI point-of-care testing in Australia. (Doctoral Dissertation). University of New South Wales. Retrieved from http://handle.unsw.edu.au/1959.4/56876 ; https://unsworks.unsw.edu.au/fapi/datastream/unsworks:41829/SOURCE02?view=true

Chicago Manual of Style (16^th Edition):

Natoli, Lisa. “Health service implications of the introduction of STI point-of-care testing in Australia.” 2015. Doctoral Dissertation, University of New South Wales. Accessed February 25, 2021. http://handle.unsw.edu.au/1959.4/56876 ; https://unsworks.unsw.edu.au/fapi/datastream/unsworks:41829/SOURCE02?view=true.

MLA Handbook (7^th Edition):

Natoli, Lisa. “Health service implications of the introduction of STI point-of-care testing in Australia.” 2015. Web. 25 Feb 2021.

Vancouver:

Natoli L. Health service implications of the introduction of STI point-of-care testing in Australia. [Internet] [Doctoral dissertation]. University of New South Wales; 2015. [cited 2021 Feb 25]. Available from: http://handle.unsw.edu.au/1959.4/56876 ; https://unsworks.unsw.edu.au/fapi/datastream/unsworks:41829/SOURCE02?view=true.

Council of Science Editors:

Natoli L. Health service implications of the introduction of STI point-of-care testing in Australia. [Doctoral Dissertation]. University of New South Wales; 2015. Available from: http://handle.unsw.edu.au/1959.4/56876 ; https://unsworks.unsw.edu.au/fapi/datastream/unsworks:41829/SOURCE02?view=true

McMaster University

13. Wolfe, Michael. Point-of-need biosensors for the detection of respiratory biomarkers.

Degree: PhD, 2019, McMaster University

URL: http://hdl.handle.net/11375/25040

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Asthma is a chronic disease affecting over 300 million people worldwide. Airway inflammation is a central feature of asthma. Quantitative sputum cytometry is the most… (more)

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Asthma is a chronic disease affecting over 300 million people worldwide. Airway inflammation is a central feature of asthma. Quantitative sputum cytometry is the most validated method to assess this and to adjust anti-inflammatory therapy, yet it is underutilized due to rigorous processing that requires expensive specialized technicians. To address these limitations, this thesis focuses on the development of several point of need biosensors that rapidly quantify respiratory biomarkers as alternatives to traditional laboratory tests. The project began by developing a paper based sensor for detection of myeloperoxidase (MPO), a neutrophil biomarker. A test was developed using commercially available antibodies, showing direct correlation between the test line colour intensity and total neutrophils. This work was expanded to include a second protein target, eosinophil peroxidase (EPX), for identification of eosinophils. Although the test performed well using neat samples, it failed to identify EPX in clinical sputum samples. Analyzing pre-treatment methods identified that a quick immunoprecipitation technique using protein A/G beads followed by syringe filtration allowed for the device to successfully quantify EPX, eliminating the need for a centrifuge. However, the limited supply of commercial anti-EPX antibodies combined with the need for sample pre-treatment prompted investigation into alternative detection avenues. Nucleic acid aptamers were explored, with aptamer selection for EPX producing several aptamer candidates. Binding affinity and specificity tests were performed, with the T1-5 aptamer emerging. T1-5 was capable of selectively binding EPX over MPO with high affinity. This aptamer was integrated into a simple pull-down assay, capable of detecting EPX with an order of magnitude lower limit of detection than the antibody test. Overall this work has developed multiple sensors with the potential to overcome the limitations of accessibility to sputum cytometry, rapidly identify the presence and type of airway inflammation, and deliver personalized treatment strategies that not only reduce the global healthcare burden, but also greatly improve a patient’s quality of life.

Thesis

Doctor of Philosophy (PhD)

Advisors/Committee Members: Brennan, John D., Chemical Biology.

Subjects/Keywords: Biosensors; Point-of-Need; Point-of-care; Asthma; Aptamer; SELEX; Antibody; Lateral Flow

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Wolfe, M. (2019). Point-of-need biosensors for the detection of respiratory biomarkers. (Doctoral Dissertation). McMaster University. Retrieved from http://hdl.handle.net/11375/25040

Chicago Manual of Style (16^th Edition):

Wolfe, Michael. “Point-of-need biosensors for the detection of respiratory biomarkers.” 2019. Doctoral Dissertation, McMaster University. Accessed February 25, 2021. http://hdl.handle.net/11375/25040.

MLA Handbook (7^th Edition):

Wolfe, Michael. “Point-of-need biosensors for the detection of respiratory biomarkers.” 2019. Web. 25 Feb 2021.

Vancouver:

Wolfe M. Point-of-need biosensors for the detection of respiratory biomarkers. [Internet] [Doctoral dissertation]. McMaster University; 2019. [cited 2021 Feb 25]. Available from: http://hdl.handle.net/11375/25040.

Council of Science Editors:

Wolfe M. Point-of-need biosensors for the detection of respiratory biomarkers. [Doctoral Dissertation]. McMaster University; 2019. Available from: http://hdl.handle.net/11375/25040

University of the Western Cape

14. Mpotulo, Nombuto Gloria. Factors affecting antenatal point of care testing for syphilis, anaemia and HIV in primary health care centres in Sedibeng district, South Africa .

Degree: 2014, University of the Western Cape

URL: http://hdl.handle.net/11394/4686

► Background: Point of Care Testing (POCT) refers to qualitative or quantitative tests done in health facilities where the patient is being attended to (on-site), and… (more)

▼ Background: Point of Care Testing (POCT) refers to qualitative or quantitative tests done in health facilities where the patient is being attended to (on-site), and not in the conventional hospital laboratory setting. As a consequence of many developing countries not having access to conventional laboratory services (with trained laboratory personnel), diagnostic testing often relies on the availability of valid POC tests. All pregnant women attending antenatal care clinics in the Sedibeng District Primary Health Care (PHC) centres should be screened for syphilis, anaemia and HIV. This can be done by means of POC testing, which is easy to perform. These POC tests provide results promptly allowing treatment to be commenced immediately, if required. Despite this highly desirable benefit of POCT, there is circumstantial evidence which suggests that staff is choosing to send specimens to the laboratory for testing, instead of doing POCT themselves. The extent to which this happens and the factors contributing to this practice are not clear. Aim: The aim of this study was to assess the prevalence of screening for syphilis, anaemia, and HIV amongst pregnant women during their first antenatal care visit to PHC facilities in the Sedibeng District, and to establish the factors affecting the prevalence of appropriately using POCT for screening tests. Methodology: Study design: A quantitative, analytical, cross-sectional study was conducted. Study Population and Sample: Patient registers, staff expected to perform POCT and facility managers. 33 District’s health care workers expected to perform POCT on pregnant women during the first ANC visit and 30 facility managers from these facilities; 360 patient records (these were collected from a total of 7 200 patients’ records). The data was collected over a six month period (from 1st July 2012 to 31st December 2012). Data collection: Data was collected from 360 patient records to determine the rate, appropriateness and mechanism of screening for syphilis, anaemia and, HIV in pregnant women on their first antenatal visit. Interviewer-administered closed-ended questions was asked from 30 antenatal care clinic staff tasked with performing POC tests and from 30 PHC facility managers to determine the factors affecting the rate of conducting POCT. Data analysis: Data was analysed using univariate, bivariate and multivariate analyses. Ethical considerations: No harm was anticipated to anyone participating in the study or from the findings of the study. A major benefit of the study was that clarity on the factors affecting the rate of screening and the use of POCT was gained. This will hopefully facilitate the implementation of evidence–based interventions to improve POCT uptake if required. Advisors/Committee Members: Reagon, Gavin (advisor).

Subjects/Keywords: Syphilis; Anaemia; HIV; Antenatal care centres; Point-of-care testing; Primary health care centres

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Mpotulo, N. G. (2014). Factors affecting antenatal point of care testing for syphilis, anaemia and HIV in primary health care centres in Sedibeng district, South Africa . (Thesis). University of the Western Cape. Retrieved from http://hdl.handle.net/11394/4686

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Mpotulo, Nombuto Gloria. “Factors affecting antenatal point of care testing for syphilis, anaemia and HIV in primary health care centres in Sedibeng district, South Africa .” 2014. Thesis, University of the Western Cape. Accessed February 25, 2021. http://hdl.handle.net/11394/4686.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Mpotulo, Nombuto Gloria. “Factors affecting antenatal point of care testing for syphilis, anaemia and HIV in primary health care centres in Sedibeng district, South Africa .” 2014. Web. 25 Feb 2021.

Vancouver:

Mpotulo NG. Factors affecting antenatal point of care testing for syphilis, anaemia and HIV in primary health care centres in Sedibeng district, South Africa . [Internet] [Thesis]. University of the Western Cape; 2014. [cited 2021 Feb 25]. Available from: http://hdl.handle.net/11394/4686.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Mpotulo NG. Factors affecting antenatal point of care testing for syphilis, anaemia and HIV in primary health care centres in Sedibeng district, South Africa . [Thesis]. University of the Western Cape; 2014. Available from: http://hdl.handle.net/11394/4686

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Universitat Politècnica de València

15. Yamanaka, Eric Seiti. Isothermal-based DNA biosensors for application in pharmacogenetics .

Degree: 2020, Universitat Politècnica de València

URL: http://hdl.handle.net/10251/148366

► [EN] The determination of genetic biomarkers is progressively becoming more extended and popular, being commercialized even in kits for personalized medicine. Establishing specific genotype variations… (more)

▼ [EN] The determination of genetic biomarkers is progressively becoming more extended and popular, being commercialized even in kits for personalized medicine. Establishing specific genotype variations for each patient, such as single nucleotide polymorphisms (SNPs), could be a fundamental tool in the field of diagnosis, prognosis and therapy selection. However, the use of DNA testing is not fully implemented in general healthcare, mainly due to technical and economic barriers associated to the current technologies, which are limited only to specialized centers and large hospitals. In this thesis, the main goal was to overcome these obstacles by developing simpler, faster and more affordable point-of-care (POC) genotyping systems. Allele discrimination was achieved by employing isothermal enzymatic reactions, like recombinase polymerase amplification (RPA), ligation of oligonucleotides and loop-mediated isothermal amplification (LAMP). These processes were integrated to colorimetric indicators and immunoenzymatic assays, in a microarray format. Using compact discs and polycarbonate chips as platforms, the detection was achieved through widespread electronics, like disc-reader, flatbed scanner and smartphone. To demonstrate their capacities, the resulting systems were applied for identifying SNPs in human samples, associated to therapies for tobacco smoking cessation, major depression disorder and blood clotting-related diseases. After selecting the proper conditions, all studied strategies discriminated SNPs in samples containing as low as 100 copies of genomic DNA, with an error rate below 15%. Most importantly, the developed methods have reduced assays times varying between 70 and 140 minutes, at a cost similar to a conventional PCR-based analog, but maintaining or raising amplification efficiency and eliminating the need of specialized temperature cyclers and fluorescence scanners. In conclusion, the biosensors based in isothermal reactions and consumer electronics devices greatly improve the competitivity of POC DNA analysis. It was demonstrated that the technologies developed in this thesis could support genotyping assays in low-resource areas, such as primary healthcare centers and emerging countries. Through this democratization of genetic testing and by performing adequate association studies, molecular diagnostics and personalized medicine practices could have their application extended to the clinical routine. Advisors/Committee Members: Maquieira Catala, Ángel (advisor), Tortajada Genaro, Luis Antonio (advisor).

Subjects/Keywords: DNA; Pharmacogenetics; Single nucleotide polymorphism; Point mutation; Point-of-care; Biosensor; Isothermal amplification; Consumer electronics

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Yamanaka, E. S. (2020). Isothermal-based DNA biosensors for application in pharmacogenetics . (Doctoral Dissertation). Universitat Politècnica de València. Retrieved from http://hdl.handle.net/10251/148366

Chicago Manual of Style (16^th Edition):

Yamanaka, Eric Seiti. “Isothermal-based DNA biosensors for application in pharmacogenetics .” 2020. Doctoral Dissertation, Universitat Politècnica de València. Accessed February 25, 2021. http://hdl.handle.net/10251/148366.

MLA Handbook (7^th Edition):

Yamanaka, Eric Seiti. “Isothermal-based DNA biosensors for application in pharmacogenetics .” 2020. Web. 25 Feb 2021.

Vancouver:

Yamanaka ES. Isothermal-based DNA biosensors for application in pharmacogenetics . [Internet] [Doctoral dissertation]. Universitat Politècnica de València; 2020. [cited 2021 Feb 25]. Available from: http://hdl.handle.net/10251/148366.

Council of Science Editors:

Yamanaka ES. Isothermal-based DNA biosensors for application in pharmacogenetics . [Doctoral Dissertation]. Universitat Politècnica de València; 2020. Available from: http://hdl.handle.net/10251/148366

16. SOARES, Erika Cristina de Lima. Desenvolvimento de testes diagnósticos para Hepatite B baseados em imunossensores .

Degree: 2016, Universidade Federal de Pernambuco

URL: https://repositorio.ufpe.br/handle/123456789/18699

► A infecção pelo vírus da Hepatite B (HBV) é considerada uma enfermidade de alta morbimortalidade, apresentando diagnóstico complexo e quadro de persistência, fatores que dificultam… (more)

▼ A infecção pelo vírus da Hepatite B (HBV) é considerada uma enfermidade de alta morbimortalidade, apresentando diagnóstico complexo e quadro de persistência, fatores que dificultam a detecção, terapêutica e cura. Relatos variados têm apontado os imunossensores como importantes ferramentas de auxílio no diagnóstico de doenças, definido como um dispositivo que converte respostas de eventos biológicos a partir da interação antígenoanticorpo em sinal elétrico. No presente estudo foram desenvolvidos biossensores para detecção de anticorpos contra o nucleocapsídeo do HBV (Anti-HBc) mais perene apresentado no diagnóstico da doença. Recentemente, o emprego de nanomateriais no desenvolvimento de tais dispositivos tem despertado interesse devido às propriedades destes materiais. Particularmente, os nanotubos de carbono (NTCs) têm oferecido aos imunossensores melhoria na condutividade, aumento na velocidade de transferência de carga, aumento da área eletródica com maior possibilidade de imobilização de biomoléculas. Nesta tese, foram empregados o ácido hialurônico e o náfion como suporte para forte interação com os NTC funcionalizados em eletrodos de carbono vítreo e de ouro fabricado sobre folha de acetato. Os dispositivos foram caracterizados por técnicas de imagem (microscopia de força atômica) e eletroquímicas (voltametrias de onda quadrada e cíclica), as quais demonstraram a estabilidade da plataforma, imobilização eficaz e sensibilidade. O primeiro protótipo em eletrodos de carbono vítreo modificado com filme de ácido hialurônico associado a nanotubos funcionalizados apresentou resposta linear de 1 a 6ng/ml com limite de detcção de 0,03ng/ml. No segundo protótipo com eletrodos impressos de ouro modificado com filme etanólico de náfion associado a nanotubos funcionalizados, o imunossensor apresentou resposta linear de 0,5 até 2ng/ml, com limite de detecção de 0,15 ng/ml de anti-HBc. Os protótipos desenvolvidos apresentam-se como potenciais para diagóstico da HBV. Advisors/Committee Members: DUTRA, Rosa Amália Fireman (advisor), http://lattes.cnpq.br/3335497739195055 (advisor).

Subjects/Keywords: Imunossensor; Nanotecnologia; Hepatite B; Dispositivos point-of-care; Diagnóstico; Immunosensor; Nanotechnology; Hepatitis B; point-of-care devices; diagnostics

Record Details Similar Records Cite « Share

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

SOARES, E. C. d. L. (2016). Desenvolvimento de testes diagnósticos para Hepatite B baseados em imunossensores . (Thesis). Universidade Federal de Pernambuco. Retrieved from https://repositorio.ufpe.br/handle/123456789/18699

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

SOARES, Erika Cristina de Lima. “Desenvolvimento de testes diagnósticos para Hepatite B baseados em imunossensores .” 2016. Thesis, Universidade Federal de Pernambuco. Accessed February 25, 2021. https://repositorio.ufpe.br/handle/123456789/18699.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

SOARES, Erika Cristina de Lima. “Desenvolvimento de testes diagnósticos para Hepatite B baseados em imunossensores .” 2016. Web. 25 Feb 2021.

Vancouver:

SOARES ECdL. Desenvolvimento de testes diagnósticos para Hepatite B baseados em imunossensores . [Internet] [Thesis]. Universidade Federal de Pernambuco; 2016. [cited 2021 Feb 25]. Available from: https://repositorio.ufpe.br/handle/123456789/18699.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

SOARES ECdL. Desenvolvimento de testes diagnósticos para Hepatite B baseados em imunossensores . [Thesis]. Universidade Federal de Pernambuco; 2016. Available from: https://repositorio.ufpe.br/handle/123456789/18699

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

17. Gosselin, David. Vers un dispositif de diagnostic point of care intégré : utilisation de la capillarité ainsi que des procédés de thermoformage et de sérigraphie. : Towards an integrated device for point-of-care diagnostics : use of capillarity with thermoforming and screen-printing processes.

Degree: Docteur es, MEP : Mécanique des fluides Energétique, Procédés, 2017, Université Grenoble Alpes (ComUE)

URL: http://www.theses.fr/2017GREAI111

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Grâce aux technologies de la microfluidique (i.e. la manipulation d'un fluide dans un système ayant une dimension caractéristique sub-millimétrique), il est possible d'imaginer l'intégration de… (more)

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Grâce aux technologies de la microfluidique (i.e. la manipulation d'un fluide dans un système ayant une dimension caractéristique sub-millimétrique), il est possible d'imaginer l'intégration de l'ensemble des fonctions ordinairement réalisées en laboratoire dans un système miniaturisé, réalisant ainsi un laboratoire sur puce. Cela peut ainsi permettre d'allier efficacité et bas-coût requis pour la réalisation de dispositif de diagnositcs médicaux utilisable en dehors d'infrastructure médicalisée, souvent appelés systèmes Point-of-Care. Pour la réalisation d'un tel dispositif, il semble important de concevoir l'intégration des différents composants du système d'une façon cohérente, et en prenant en compte l'ensemble des contraintes imposées par l'application finale ciblée. Le travail effectué au cours de cette thèse a ainsi été réalisé dans l'optique de proposer une réponse à cette problématique d'intégration dans le cadre du développement d'un système microfluidique de diagnostic Point-of-Care basé sur une réaction d'amplification d'ADN isotherme LAMP. Afin de pouvoir proposer un système bon marché et dont l'industrialisation est aisée, nous avons fait appel à l'utilisation du papier comme support et au thermoformage comme moyen de production. En effet à la fois l'industrie papetière et le procédé de thermoformage sont d'ores et déjà existant et proposent des fabrications en série. De plus, le faible coût du matériau et du procédé en question permettent d'envisager un dispositif final à bas-coût. Afin de pouvoir effectuer et détecter la réaction de LAMP la présence de fonctions actives telles qu'un chauffage et un outil de détection est nécessaire. Pour ces dernières, l'intégration a été réalisée par procédé sérigraphique. Le chauffage est effectué par effet Joule grâce au dépôt d'une couche d'encre conductrice à base de carbone. La détection est quant à elle faite par méthode potentiométrique, à l'aide d'électrode couverte de polyaniline. Il sera également montré que l'utilisation de ces méthodes de fabrication est pertinente en termes d'intégration car elles permettent une superposition des différentes fonctions actives, mais également leur intégration directement dans le système microfluidique.

Developments of microfluidics - the study of flows at the sub-millimetric dimensions - have made possible the integration of most of the macroscopic functions of laboratory fluidic systems in a miniaturized system, thus realizing a lab on a chip. This allows the conception of low cost, sensitive and efficient medical diagnostic device usable outside of a medical infrastructure. Such devices are called Point-of-Care (PoC) systems. The design and fabrication of such devices requires an elaborated and coherent integration that takes into account all the constraints imposed by the targeted final application.The work reported here, and performed during the PhD internship, is focused on the study of the concept and development of the integration of a PoC device based on the isothermal LAMP (Loop mediated AMPlification)…

Advisors/Committee Members: Chaussy, Didier (thesis director), Belgacem, Naceur (thesis director).

Subjects/Keywords: Microfluidique; Intégration; Capillarité; Point-Of-Care; Sérigraphie; Thermoformage; Microfluidic; Integration; Capillarity; Point-Of-Care; Screen-Printing; Thermoforming; 620

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Gosselin, D. (2017). Vers un dispositif de diagnostic point of care intégré : utilisation de la capillarité ainsi que des procédés de thermoformage et de sérigraphie. : Towards an integrated device for point-of-care diagnostics : use of capillarity with thermoforming and screen-printing processes. (Doctoral Dissertation). Université Grenoble Alpes (ComUE). Retrieved from http://www.theses.fr/2017GREAI111

Chicago Manual of Style (16^th Edition):

Gosselin, David. “Vers un dispositif de diagnostic point of care intégré : utilisation de la capillarité ainsi que des procédés de thermoformage et de sérigraphie. : Towards an integrated device for point-of-care diagnostics : use of capillarity with thermoforming and screen-printing processes.” 2017. Doctoral Dissertation, Université Grenoble Alpes (ComUE). Accessed February 25, 2021. http://www.theses.fr/2017GREAI111.

MLA Handbook (7^th Edition):

Gosselin, David. “Vers un dispositif de diagnostic point of care intégré : utilisation de la capillarité ainsi que des procédés de thermoformage et de sérigraphie. : Towards an integrated device for point-of-care diagnostics : use of capillarity with thermoforming and screen-printing processes.” 2017. Web. 25 Feb 2021.

Vancouver:

Gosselin D. Vers un dispositif de diagnostic point of care intégré : utilisation de la capillarité ainsi que des procédés de thermoformage et de sérigraphie. : Towards an integrated device for point-of-care diagnostics : use of capillarity with thermoforming and screen-printing processes. [Internet] [Doctoral dissertation]. Université Grenoble Alpes (ComUE); 2017. [cited 2021 Feb 25]. Available from: http://www.theses.fr/2017GREAI111.

Council of Science Editors:

Gosselin D. Vers un dispositif de diagnostic point of care intégré : utilisation de la capillarité ainsi que des procédés de thermoformage et de sérigraphie. : Towards an integrated device for point-of-care diagnostics : use of capillarity with thermoforming and screen-printing processes. [Doctoral Dissertation]. Université Grenoble Alpes (ComUE); 2017. Available from: http://www.theses.fr/2017GREAI111

18. Huet, Maxime. Agglutination de globules rouges autologues par un réactif bispécifique pour le dosage de biomarqueurs : Autologous red blood cells agglutination by a bispecific reagent for the quantification of biomarker.

Degree: Docteur es, Physique pour les sciences du vivant, 2016, Université Grenoble Alpes (ComUE)

URL: http://www.theses.fr/2016GREAY098

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La détection ou la quantification dans le sang de biomarqueurs peut apporter une précieuse information sur la santé humaine. Cette analyse peut être réalisée directement… (more)

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La détection ou la quantification dans le sang de biomarqueurs peut apporter une précieuse information sur la santé humaine. Cette analyse peut être réalisée directement auprès du patient, on parle alors de POC (point-of-care). L’agglutination de globules rouges par un réactif bispécifique, ciblant d’une part un globule rouge et d’autre part le biomarqueur à doser ou détecter, est proposée comme principe de base d’un test POC autonome et quantitatif. L’automatisation du protocole d’agglutination en microfluidique, ainsi que la mesure optique de la cinétique de l’agglutination sont explorées selon trois questions. La première concerne la possibilité de produire de manière autonome et reproductible l’agglutination en microfluidique passive, c’est-à-dire sans apport d’énergie ni de matière autre que l’échantillon. Les deuxième et troisième questions concernent la mesure de la cinétique d’agglutination et l’existence d’un lien entre cette mesure et la concentration du biomarqueur. La formulation et l’embarquement du réactif se sont révélés indispensables pour effectuer une réaction d’agglutination de manière reproductible en microfluidique passive et répondre à la première question. Trois stratégies de mesure de l’agglutination basées sur les propriétés optiques des globules rouges ont été proposées. Deux d’entre elles ont pu être implémentées avec succès. La mesure cinétique de l’agglutination a été mise en place pour un modèle de typage sanguin et a permis la discrimination entre positif et négatif dans 100 % des cas d’agglutinations testés. L’effet de la concentration du biomarqueur sur la mesure de l’agglutination avec un réactif bispécifique a été démontré avec le modèle du biomarqueur D-dimère, répondant à la dernière question posée en début de thèse.

The detection or quantification of biomarkers in the blood can provide valuable information on human health. An analysis directly performed at the patient bedside is called a Point-of-care test (POC). The agglutination of red blood cells by a bispecific reagent combining a biomarker binding part and an erythrocyte binding part is proposed as a basis for an autonomous and quantitative POC test. The integration and automation of the protocol in a microfluidic chip and the optical measurement of the kinetics of agglutination are investigated. The first question concerns the possibility of producing agglutination in passive microfluidic device that is to say without any energy nor any material supply other than the sample. The second and third questions respectively relate to the measurement of the kinetics of aggregation and the existence of a link between this measure and the concentration of the biomarker. The formulation and embedding of the reagents has proved essential to perform a reproducible agglutination reaction in passive microfluidics and thus answer the first question. Three measurement strategies based on the optical properties of the red blood cells have been proposed. Two of them have been successfully implemented. The kinetic measurement of…

Advisors/Committee Members: Buhot, Arnaud (thesis director).

Subjects/Keywords: Microfluidique; Point-Of-Care; Sang; Agglutination; Dosage; Traitement d'image; Microfluidic; Point-Of-Care; Blood; Agglutination; Quantification; Image processing; 530

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Huet, M. (2016). Agglutination de globules rouges autologues par un réactif bispécifique pour le dosage de biomarqueurs : Autologous red blood cells agglutination by a bispecific reagent for the quantification of biomarker. (Doctoral Dissertation). Université Grenoble Alpes (ComUE). Retrieved from http://www.theses.fr/2016GREAY098

Chicago Manual of Style (16^th Edition):

Huet, Maxime. “Agglutination de globules rouges autologues par un réactif bispécifique pour le dosage de biomarqueurs : Autologous red blood cells agglutination by a bispecific reagent for the quantification of biomarker.” 2016. Doctoral Dissertation, Université Grenoble Alpes (ComUE). Accessed February 25, 2021. http://www.theses.fr/2016GREAY098.

MLA Handbook (7^th Edition):

Huet, Maxime. “Agglutination de globules rouges autologues par un réactif bispécifique pour le dosage de biomarqueurs : Autologous red blood cells agglutination by a bispecific reagent for the quantification of biomarker.” 2016. Web. 25 Feb 2021.

Vancouver:

Huet M. Agglutination de globules rouges autologues par un réactif bispécifique pour le dosage de biomarqueurs : Autologous red blood cells agglutination by a bispecific reagent for the quantification of biomarker. [Internet] [Doctoral dissertation]. Université Grenoble Alpes (ComUE); 2016. [cited 2021 Feb 25]. Available from: http://www.theses.fr/2016GREAY098.

Council of Science Editors:

Huet M. Agglutination de globules rouges autologues par un réactif bispécifique pour le dosage de biomarqueurs : Autologous red blood cells agglutination by a bispecific reagent for the quantification of biomarker. [Doctoral Dissertation]. Université Grenoble Alpes (ComUE); 2016. Available from: http://www.theses.fr/2016GREAY098

19. Michel-Lepage, Audrey. Améliorer la prescription médicamenteuse grâce à des mécanismes économiques? : Improving drug prescription thanks to economic mechanisms.

Degree: Docteur es, Sciences économiques, 2016, Aix Marseille Université

URL: http://www.theses.fr/2016AIXM2028

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Le mésusage des antibiotiques et l’antibio-résistance sont devenus un cheval de bataille des autorités sanitaires internationales. L’objectif de ma thèse est d’analyser et évaluer les… (more)

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Le mésusage des antibiotiques et l’antibio-résistance sont devenus un cheval de bataille des autorités sanitaires internationales. L’objectif de ma thèse est d’analyser et évaluer les différents types d’incitations qui amèneraient les médecins à « mieux » prescrire des antibiotiques. La première partie est une revue de littérature dans laquelle j’analyse les interactions entre les différents acteurs du système de santé français, les incitations monétaires et les incitations non-monétaires. Les seconde et troisième parties illustrent la première par des analyses principalement empiriques, qui ont été publiées ou envoyées à des revues. La deuxième partie étudie l’utilisation et l’impact d’outils diagnostiques comme aide à la prescription d’antibiotiques avec d’une part les tests de diagnostics rapides pour l’angine, et d’autre part les tests microbiologiques point-of-care, faits « au lit du patient ». La troisième partie analyse le paiement à la performance comme incitation monétaire à l’amélioration des prescriptions médicamenteuses et notamment des antibiotiques.

Antibiotic misuse and antibiotic resistance are increasing concern for international health authorities. The goal of my thesis is to assess the different incentives which would lead physicians to optimize their antibiotic prescriptions. The first part is a literature review analysing interactions between the agents of the French health system. I evaluate also financial and non-monetary incentives. The second and third parts illustrates the first one through empiric analyses, which have been published or sent to a review. The second part studies the use and the impact of diagnostic tests as a help for antibiotics prescribing, with on one hand the use of rapid diagnostic tests detecting tonsillitis, and on the other hand microbiological tests called Point-Of-care made at the patient bedside. The third part analyses the pay-for-performance program as a financial incentive for improving drugs prescriptions and notably antibiotics.

Advisors/Committee Members: Ventelou, Bruno (thesis director), Drancourt, Michel (thesis director).

Subjects/Keywords: Incitations; Prescription; Point Of Care; Capi; Antibiotiques; Comportement; Médecins; Incentives; Prescription; Point of care; Capi; Antibiotics; Behaviours; General practitioners

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Michel-Lepage, A. (2016). Améliorer la prescription médicamenteuse grâce à des mécanismes économiques? : Improving drug prescription thanks to economic mechanisms. (Doctoral Dissertation). Aix Marseille Université. Retrieved from http://www.theses.fr/2016AIXM2028

Chicago Manual of Style (16^th Edition):

Michel-Lepage, Audrey. “Améliorer la prescription médicamenteuse grâce à des mécanismes économiques? : Improving drug prescription thanks to economic mechanisms.” 2016. Doctoral Dissertation, Aix Marseille Université. Accessed February 25, 2021. http://www.theses.fr/2016AIXM2028.

MLA Handbook (7^th Edition):

Michel-Lepage, Audrey. “Améliorer la prescription médicamenteuse grâce à des mécanismes économiques? : Improving drug prescription thanks to economic mechanisms.” 2016. Web. 25 Feb 2021.

Vancouver:

Michel-Lepage A. Améliorer la prescription médicamenteuse grâce à des mécanismes économiques? : Improving drug prescription thanks to economic mechanisms. [Internet] [Doctoral dissertation]. Aix Marseille Université 2016. [cited 2021 Feb 25]. Available from: http://www.theses.fr/2016AIXM2028.

Council of Science Editors:

Michel-Lepage A. Améliorer la prescription médicamenteuse grâce à des mécanismes économiques? : Improving drug prescription thanks to economic mechanisms. [Doctoral Dissertation]. Aix Marseille Université 2016. Available from: http://www.theses.fr/2016AIXM2028

Nelson Mandela Metropolitan University

20. Van Rooyen, Annesty Elaine. Experiences of medical practitioners regarding the accessing of information at the point-of-care via mobile technology for clinical decision making at public hospitals.

Degree: Faculty of Health Sciences, 2016, Nelson Mandela Metropolitan University

URL: http://hdl.handle.net/10948/5554

► Medical practitioners are often unable to access medical and health information at the point-of-care, thus preventing them from providing quality healthcare. Family Health International 360… (more)

▼ Medical practitioners are often unable to access medical and health information at the point-of-care, thus preventing them from providing quality healthcare. Family Health International 360 (FHI) provided medical practitioners with a locally relevant, reliable, and accurate comprehensive library of medical information on mobile computing devices (MCDs), at the point-of-care, as part of a project in collaboration with the Department of Health in the Eastern Cape Province. As part of the latter project, Ricks (2012:7) conducted an investigation into the impact that accessing health information at the point-of-care, via MCDs, had on the clinical decision-making practice of medical practitioners and professional nurses in public hospitals and primary healthcare settings in the Eastern Cape Province. The researcher identified a gap in the aforementioned study and was thus motivated to conduct this study to explore and describe the experiences of medical practitioners at public hospitals in further detail by conducting a qualitative study, as the previous study was quantitative. The purpose of this study was therefore to explore and describe the experiences of medical practitioners regarding the accessing of information at the point-of-care, via mobile technology, for clinical decision making at public hospitals. To achieve the purpose of the study, a qualitative, explorative, descriptive and contextual research design was used. The research population comprised medical practitioners who were using MCDs to access information at the point-of-care for clinical decision making. Purposive sampling was used to select the research sample. Semi-structured interviews were used to collect the necessary research data. Tesch’s steps were used to analyse the data. The principles for ensuring trustworthiness and ethical considerations were adhered to throughout the study. Two main themes and six sub-themes emerged in relation to the experiences of medical practitioners regarding the accessing of information at the point-of-care, for clinical decision making, via mobile technology. The main findings of the research highlighted the benefits and challenges that were experienced by the medical practitioners when using the MCDs for accessing information at the point-of-care for clinical decision making. The study concludes with recommendations pertaining to the areas of practise, education and research.

Subjects/Keywords: Clinical medicine – Decision making; Point-of-care testing; Mobile computing

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Van Rooyen, A. E. (2016). Experiences of medical practitioners regarding the accessing of information at the point-of-care via mobile technology for clinical decision making at public hospitals. (Thesis). Nelson Mandela Metropolitan University. Retrieved from http://hdl.handle.net/10948/5554

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Van Rooyen, Annesty Elaine. “Experiences of medical practitioners regarding the accessing of information at the point-of-care via mobile technology for clinical decision making at public hospitals.” 2016. Thesis, Nelson Mandela Metropolitan University. Accessed February 25, 2021. http://hdl.handle.net/10948/5554.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Van Rooyen, Annesty Elaine. “Experiences of medical practitioners regarding the accessing of information at the point-of-care via mobile technology for clinical decision making at public hospitals.” 2016. Web. 25 Feb 2021.

Vancouver:

Van Rooyen AE. Experiences of medical practitioners regarding the accessing of information at the point-of-care via mobile technology for clinical decision making at public hospitals. [Internet] [Thesis]. Nelson Mandela Metropolitan University; 2016. [cited 2021 Feb 25]. Available from: http://hdl.handle.net/10948/5554.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Van Rooyen AE. Experiences of medical practitioners regarding the accessing of information at the point-of-care via mobile technology for clinical decision making at public hospitals. [Thesis]. Nelson Mandela Metropolitan University; 2016. Available from: http://hdl.handle.net/10948/5554

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of California – Irvine

21. Kong, Ling Xuan. Fully Integrated Molecular Diagnostic CD Platform Based on Thermal Control.

Degree: Biomedical Engineering, 2015, University of California – Irvine

URL: http://www.escholarship.org/uc/item/7wr9v6zj

► Centrifugal microfluidics, or compact disc (CD) microfluidics, has been gaining popularity in the lab-on-a-chip field as an advanced diagnostic platform over the last fifteen years.… (more)

Subjects/Keywords: Biomedical engineering; centrifugal; diagnostics; microfluidics; molecular; point-of-care; thermal

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Kong, L. X. (2015). Fully Integrated Molecular Diagnostic CD Platform Based on Thermal Control. (Thesis). University of California – Irvine. Retrieved from http://www.escholarship.org/uc/item/7wr9v6zj

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Kong, Ling Xuan. “Fully Integrated Molecular Diagnostic CD Platform Based on Thermal Control.” 2015. Thesis, University of California – Irvine. Accessed February 25, 2021. http://www.escholarship.org/uc/item/7wr9v6zj.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Kong, Ling Xuan. “Fully Integrated Molecular Diagnostic CD Platform Based on Thermal Control.” 2015. Web. 25 Feb 2021.

Vancouver:

Kong LX. Fully Integrated Molecular Diagnostic CD Platform Based on Thermal Control. [Internet] [Thesis]. University of California – Irvine; 2015. [cited 2021 Feb 25]. Available from: http://www.escholarship.org/uc/item/7wr9v6zj.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Kong LX. Fully Integrated Molecular Diagnostic CD Platform Based on Thermal Control. [Thesis]. University of California – Irvine; 2015. Available from: http://www.escholarship.org/uc/item/7wr9v6zj

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of California – Berkeley

22. Yeh, Erh-Chia. Integrated Microfluidic Molecular Diagnostics for Point-of-Care.

Degree: Bioengineering, 2015, University of California – Berkeley

URL: http://www.escholarship.org/uc/item/99s48767

► Ideal point-of-care medical diagnostic devices are low cost assays capable of performing quantitative on-site rapid testing with high sensitivity and minimal manual steps. Current mainstream… (more)

▼ Ideal point-of-care medical diagnostic devices are low cost assays capable of performing quantitative on-site rapid testing with high sensitivity and minimal manual steps. Current mainstream assays have several key limitations. Take, for instance, the common lateral flow assay—e.g. the pregnancy dipstick test. Such assays produce rapid results at low cost; however, they are mostly qualitative tests yielding only positive/negative results rather than quantitative figures. Other standard immunosorbant assays such as ELISA yield quantitative results but require several hours and extensive manual operation. At the other end of the spectrum, nucleic acid amplification techniques such as quantitative real-time PCR can deliver much higher sensitivity and selectivity. Unfortunately, these require costly equipment and several sample preparation steps. In this thesis, an integrated low-cost microfluidic chip and peripheral technologies for quantitative molecular diagnostics is described. These technical advances are designed to address the prevailing dilemmas described above. Researchers have developed and integrated several key components with microfluidic lab-on-chip miniaturization technology. In line with cutting-edge technology, a novel reagent patterning method, termed “digital micro-patterning”, was developed. A very simple method, it can be adopted at low-resource laboratory settings with mainstream equipment. Digital micro-patterning is unique in the sense that it can digitally pattern and concentrate reagents into highly defined micro-patterns. As a proof of concept, it was possible to pattern isothermal amplification reagents in hundreds of microwells and run amplification reactions in these wells. Next, a next-generation passive microfluidic pumping technology, termed the “vacuum battery system”, has been developed. This system allows for precise passive microfluidic pumping without external pumps, controls, or power sources for up to several hours. It does not require opaque fibers as in capillary systems (e.g. lateral flow assays), thus rendering this pumping method very attractive for optical detection platforms. The vacuum battery system is also significantly more robust compared to previous degas pumping techniques. Due to its portability, excellent optical properties, low cost, and the ability for complete integration with microfluidics, this platform technology opens exciting new opportunities to create a nouveau generation of standalone microfluidic chips readily operable in field settings. Additionally, a microfluidic sample preparation technology termed “digital plasma separation” has been developed. This technology uses parallel micro-cliff-like structures and gravity sedimentation to simultaneously separate plasma and compartmentalize samples into hundreds of micro-wells within minutes. Such sample preparation method enables isothermal digital nucleic acid amplification in one step. As a proof of concept, these technologies were integrated into a single microfluidic chip, termed the…

Subjects/Keywords: Biomedical engineering; diagnostics; digital; microfluidics; Point-of-Care; pumping; sample preparation

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Yeh, E. (2015). Integrated Microfluidic Molecular Diagnostics for Point-of-Care. (Thesis). University of California – Berkeley. Retrieved from http://www.escholarship.org/uc/item/99s48767

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Yeh, Erh-Chia. “Integrated Microfluidic Molecular Diagnostics for Point-of-Care.” 2015. Thesis, University of California – Berkeley. Accessed February 25, 2021. http://www.escholarship.org/uc/item/99s48767.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Yeh, Erh-Chia. “Integrated Microfluidic Molecular Diagnostics for Point-of-Care.” 2015. Web. 25 Feb 2021.

Vancouver:

Yeh E. Integrated Microfluidic Molecular Diagnostics for Point-of-Care. [Internet] [Thesis]. University of California – Berkeley; 2015. [cited 2021 Feb 25]. Available from: http://www.escholarship.org/uc/item/99s48767.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Yeh E. Integrated Microfluidic Molecular Diagnostics for Point-of-Care. [Thesis]. University of California – Berkeley; 2015. Available from: http://www.escholarship.org/uc/item/99s48767

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

UCLA

23. Bristow, Claire C. Contributions to the improvement and understanding of new diagnostics for HIV infection and syphilis.

Degree: Epidemiology, 2015, UCLA

URL: http://www.escholarship.org/uc/item/42p95081

► Syphilis is a curable disease, yet 10 million persons worldwide are infected each year, of which 1.4 million are pregnant women. Syphilis is an infection… (more)

▼ Syphilis is a curable disease, yet 10 million persons worldwide are infected each year, of which 1.4 million are pregnant women. Syphilis is an infection caused by the spirochete Treponema pallidum and frequently has atypical presentations that may be difficult to differentiate from other sexually transmitted infections (STIs) making effective diagnostics essential to the identification of infection. Additionally, the manifestations of the disease vary depending on the duration of infection and time of presentation. There are four stages-primary, secondary, latent (early and late), and tertiary. Syphilis screening and treatment programs that utilize laboratory-based testing have been hampered by limited laboratory access, long turn-around time for results, and loss to follow-up of syphilis infected individuals. When syphilis diagnostic testing involves multiple tests performed off-site, only a proportion of infected individuals receive treatment and continued transmission occurs. A rapid test is a simple point-of-care test that can be used in a variety of settings and provides a result to guide clinical management during the time of the initial consultation (ideally within 30 minutes or less). The advent of rapid point-of-care tests for syphilis has reduced barriers and allowed for new health systems approaches to syphilis prevention, including same day testing and treatment. Dual rapid tests that have multiple analytes for the detection of antibodies for both HIV and syphilis infections are now available.Our first study addressed preferences for dual HIV/syphilis tests through a conjoint survey analysis. Conjoint analysis is a method for systematically estimating consumer preferences across discrete attributes. Conjoint analysis has been used extensively in marketing research and measures the value that consumers place on each feature of a product. Conjoint analysis has recently begun to be applied in health research. We recruited 298 men and women 18 years of age and over seeking testing or care at GHESKIO (Haitian Study Group for Kaposi’s sarcoma and Opportunistic Infections) clinics. We created 8 hypothetical dual test profiles varying across six dichotomous attributes: cost (free versus 4), accuracy (no false positive versus false positive), time-to-result (20 minutes versus 1 week), blood draw method (finger prick versus venipuncture), number of draws (1 versus 2), and test type (rapid versus laboratory). Cost (free vs. 4; impact score=27.2, SD=36.6, p<.0001) had the highest impact on likelihood of testing, followed by number of blood draws (1 versus 2; impact score=17.5, SD=29.8, p<.0001), blood draw method (fingerprick versus venipuncture; impact score=9.7, SD=26.5, p<.0001), test type (rapid versus laboratory; impact score= -4.5, SD=21.9 , P=.0005), and time-to-result (20 minutes versus 1 week; impact score=3.6, SD=25.6, p=.0139). This analysis showed that implementation of a low cost dual rapid test in the laboratory for HIV and syphilis could be used to improve screening uptake and accessibility to…

Subjects/Keywords: Epidemiology; diagnostics; HIV; point-of-care testing; Syphilis

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Bristow, C. C. (2015). Contributions to the improvement and understanding of new diagnostics for HIV infection and syphilis. (Thesis). UCLA. Retrieved from http://www.escholarship.org/uc/item/42p95081

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Bristow, Claire C. “Contributions to the improvement and understanding of new diagnostics for HIV infection and syphilis.” 2015. Thesis, UCLA. Accessed February 25, 2021. http://www.escholarship.org/uc/item/42p95081.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Bristow, Claire C. “Contributions to the improvement and understanding of new diagnostics for HIV infection and syphilis.” 2015. Web. 25 Feb 2021.

Vancouver:

Bristow CC. Contributions to the improvement and understanding of new diagnostics for HIV infection and syphilis. [Internet] [Thesis]. UCLA; 2015. [cited 2021 Feb 25]. Available from: http://www.escholarship.org/uc/item/42p95081.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Bristow CC. Contributions to the improvement and understanding of new diagnostics for HIV infection and syphilis. [Thesis]. UCLA; 2015. Available from: http://www.escholarship.org/uc/item/42p95081

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Rochester

24. Bonanno, Lisa (1982 - ). Advancing porous silicon biosensor technology for use in clinical diagnostics.

Degree: PhD, 2011, University of Rochester

URL: http://hdl.handle.net/1802/14133

► Inexpensive and robust analytical techniques for detecting molecular recognition events are in great demand in healthcare, food safety, and environmental monitoring. Despite vast research in… (more)

▼ Inexpensive and robust analytical techniques for detecting molecular recognition events are in great demand in healthcare, food safety, and environmental monitoring. Despite vast research in this area, challanges remain to develop practical biomolecular platforms that meet the rigorous demands of real-world applications. This includes maintaining low-cost devices that are sensitive and specific in complex test specimens, are stable after storage, have short assay time, and possess minimal complexity of instrumentation for readout. Nanostructured porous silicon (PSi) material has been identified as an ideal candidate towards achieving these goals and the past decade has seen diverse proof-of-principle studies developing optical-based sensing techniques. In Part 1 of this thesis, the impact of surface chemistry and PSi morphology on detection sensitivity of target molecules is investigated. Initial proof-of-concept that PSi devices facilitate detection of protein in whole blood is demonstrated. This work highlights the importance of material stability and blocking chemistry for sensor use in real world biological samples. In addition, the intrinisic filtering capability of the 3-D PSi morphology is shown as an advantage in complex solutions, such as whole blood. Ultimately, this initial work identified a need to improve detection sensitivity of the PSi biosensor technique to facilitate clinical diagnostic use over relevant target concentration ranges. The second part of this thesis, builds upon sensitivity challenges that are highlighted in the first part of the thesis and development of a surface-bound competitive inhibition immunoassay facilitated improved detection sensitivity of small molecular weight targets (opiates) over a relevant clinical concentration range. In addition, optimization of assay protocol addressed issues of maintaining stability of sensors after storage. Performance of the developed assay (specificity and sensitivity) was then validated in a blind clinical study that screened real patient urine samples (n=70) for opiates in collaboration with Strong Memorial Hospital Clinical Toxicology Laboratory. PSi sensor results showed improved clinical specificity over current commercial opiate immunoassay techniques and therefore, identified potential for a reduction in false-negative and false-positive screening results. Here, we demonstrate for the first time, successful clinical capability of a PSi sensor to detect opiates as a model target in real-world patient samples. The final part of this thesis explores novel sensor designs to leverage the tunable optical properties of PSi photonic devices and facilitate colorimetric readout of molecular recognition events by the unaided eye. Such a design is ideal for uncomplicated diagnostic screening at point-of-care as no instrumentation is needed for result readout. The photonic PSi transducers were integrated with target analyte-responsive hydrogels (TRAP-gels) that upon exposure to a target solution would swell and…

Subjects/Keywords: Sensor; Point-of-care diagnostic; Responsive hydrogel; Porous silicon; Immunoassay

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APA (6^th Edition):

Bonanno, L. (. -. ). (2011). Advancing porous silicon biosensor technology for use in clinical diagnostics. (Doctoral Dissertation). University of Rochester. Retrieved from http://hdl.handle.net/1802/14133

Chicago Manual of Style (16^th Edition):

Bonanno, Lisa (1982 - ). “Advancing porous silicon biosensor technology for use in clinical diagnostics.” 2011. Doctoral Dissertation, University of Rochester. Accessed February 25, 2021. http://hdl.handle.net/1802/14133.

MLA Handbook (7^th Edition):

Bonanno, Lisa (1982 - ). “Advancing porous silicon biosensor technology for use in clinical diagnostics.” 2011. Web. 25 Feb 2021.

Vancouver:

Bonanno L(-). Advancing porous silicon biosensor technology for use in clinical diagnostics. [Internet] [Doctoral dissertation]. University of Rochester; 2011. [cited 2021 Feb 25]. Available from: http://hdl.handle.net/1802/14133.

Council of Science Editors:

Bonanno L(-). Advancing porous silicon biosensor technology for use in clinical diagnostics. [Doctoral Dissertation]. University of Rochester; 2011. Available from: http://hdl.handle.net/1802/14133

Rochester Institute of Technology

25. Hass, Kenneth N. For the Sensing of Viral DNA: An Integrated Polydimethylsiloxane Accurate CRISPR Detection (IMPACT) System.

Degree: MS, Mechanical Engineering, 2020, Rochester Institute of Technology

URL: https://scholarworks.rit.edu/theses/10347

► Infectious disease outbreaks have become more frequent and extreme in recent years, and as populations continue to grow and the world becomes more interconnected,… (more)

Subjects/Keywords: CRISPR; DNA; Fluorescence; Microfluidics; Micropillar; Point-of-care

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APA (6^th Edition):

Hass, K. N. (2020). For the Sensing of Viral DNA: An Integrated Polydimethylsiloxane Accurate CRISPR Detection (IMPACT) System. (Masters Thesis). Rochester Institute of Technology. Retrieved from https://scholarworks.rit.edu/theses/10347

Chicago Manual of Style (16^th Edition):

Hass, Kenneth N. “For the Sensing of Viral DNA: An Integrated Polydimethylsiloxane Accurate CRISPR Detection (IMPACT) System.” 2020. Masters Thesis, Rochester Institute of Technology. Accessed February 25, 2021. https://scholarworks.rit.edu/theses/10347.

MLA Handbook (7^th Edition):

Hass, Kenneth N. “For the Sensing of Viral DNA: An Integrated Polydimethylsiloxane Accurate CRISPR Detection (IMPACT) System.” 2020. Web. 25 Feb 2021.

Vancouver:

Hass KN. For the Sensing of Viral DNA: An Integrated Polydimethylsiloxane Accurate CRISPR Detection (IMPACT) System. [Internet] [Masters thesis]. Rochester Institute of Technology; 2020. [cited 2021 Feb 25]. Available from: https://scholarworks.rit.edu/theses/10347.

Council of Science Editors:

Hass KN. For the Sensing of Viral DNA: An Integrated Polydimethylsiloxane Accurate CRISPR Detection (IMPACT) System. [Masters Thesis]. Rochester Institute of Technology; 2020. Available from: https://scholarworks.rit.edu/theses/10347

University of Alberta

26. Genoway, Shyla G.R. A Narrative Inquiry into the Experiences of Point-of-Care HIV Testing Alongside People who were Tested while in a Correctional Facility or at a Bathhouse.

Degree: Master of Nursing, Faculty of Nursing, 2015, University of Alberta

URL: https://era.library.ualberta.ca/files/765373878

► With a call to increase the accessibility of HIV testing in Canada, point-of-care testing for HIV is being readily adopted. The World Health Organization (WHO,… (more)

Subjects/Keywords: narrative inquiry; correctional facility; Point of care; bathhouse; HIV

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APA (6^th Edition):

Genoway, S. G. R. (2015). A Narrative Inquiry into the Experiences of Point-of-Care HIV Testing Alongside People who were Tested while in a Correctional Facility or at a Bathhouse. (Masters Thesis). University of Alberta. Retrieved from https://era.library.ualberta.ca/files/765373878

Chicago Manual of Style (16^th Edition):

Genoway, Shyla G R. “A Narrative Inquiry into the Experiences of Point-of-Care HIV Testing Alongside People who were Tested while in a Correctional Facility or at a Bathhouse.” 2015. Masters Thesis, University of Alberta. Accessed February 25, 2021. https://era.library.ualberta.ca/files/765373878.

MLA Handbook (7^th Edition):

Genoway, Shyla G R. “A Narrative Inquiry into the Experiences of Point-of-Care HIV Testing Alongside People who were Tested while in a Correctional Facility or at a Bathhouse.” 2015. Web. 25 Feb 2021.

Vancouver:

Genoway SGR. A Narrative Inquiry into the Experiences of Point-of-Care HIV Testing Alongside People who were Tested while in a Correctional Facility or at a Bathhouse. [Internet] [Masters thesis]. University of Alberta; 2015. [cited 2021 Feb 25]. Available from: https://era.library.ualberta.ca/files/765373878.

Council of Science Editors:

Genoway SGR. A Narrative Inquiry into the Experiences of Point-of-Care HIV Testing Alongside People who were Tested while in a Correctional Facility or at a Bathhouse. [Masters Thesis]. University of Alberta; 2015. Available from: https://era.library.ualberta.ca/files/765373878

Cornell University

27. Oncescu, Vlad-Victor. Development Of Point-Of-Care Devices For Rapid Diagnostics And Preventive Care.

Degree: PhD, Mechanical Engineering, 2014, Cornell University

URL: http://hdl.handle.net/1813/36188

► With the cost of healthcare in the U.S. predicted to reach 30% of the GDP by 2040, medical technology needs to help reduce the stress… (more)

▼ With the cost of healthcare in the U.S. predicted to reach 30% of the GDP by 2040, medical technology needs to help reduce the stress on physicians and facilitate personalized preventive care. The two most promising ways of achieving this are through developments in implantable devices for monitoring and treating patients outside of clinical settings and through better point-of-care diagnostics tools. The first part of this dissertation focuses on the development of a potentially implantable autonomous device for the prevention of late-phase hemorrhagic shock (HS), the leading cause of death for people with traumatic injuries. We demonstrate that such a device can continuously monitor vasopressin levels, an indicator of late-phase HS, and release vasopressin automatically when levels drop below a certain threshold in order to help stabilize the situation. We also discuss the possibility of using a nonenzymatic glucose fuel cell unit, instead of a lithium battery, in order to increase the implantable device's lifetime. Novel power sources are important in the development of low-power long-term implantable devices, and we propose several non-enzymatic fuel cells that can be used as coating layers on current implantable devices or as stand-alone power sources. We show that such glucose fuel cell can produce 16[MICRO SIGN]W cm-3 of power and can be integrated in implantable devices such as the one for preventing late-phase HS. In the second part of the dissertation, we discuss the development of a platform for colorimetric biomarker detection on a smartphone. This platform consists of a smartphone accessory that allows uniform and repeatable image acquisition of a colorimetric test strip and an app that analyzes parameters such as hue, saturation and luminosity of the test area, quantifies the biomarker levels and displays the value on the screen. We demonstrate its use in monitoring electrolyte loss, enamel decalcification, cholesterol and vitamin D. We envision this as the first step toward the development of the NutriPhone, a platform for vitamin and micronutrient testing on a smartphone. Advisors/Committee Members: Erickson, David (chair), Kan, Edwin Chihchuan (committee member), Abruna, Hector D (committee member).

Subjects/Keywords: point-of-care; smartphone diagnostics; glucose fuel cells

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APA (6^th Edition):

Oncescu, V. (2014). Development Of Point-Of-Care Devices For Rapid Diagnostics And Preventive Care. (Doctoral Dissertation). Cornell University. Retrieved from http://hdl.handle.net/1813/36188

Chicago Manual of Style (16^th Edition):

Oncescu, Vlad-Victor. “Development Of Point-Of-Care Devices For Rapid Diagnostics And Preventive Care.” 2014. Doctoral Dissertation, Cornell University. Accessed February 25, 2021. http://hdl.handle.net/1813/36188.

MLA Handbook (7^th Edition):

Oncescu, Vlad-Victor. “Development Of Point-Of-Care Devices For Rapid Diagnostics And Preventive Care.” 2014. Web. 25 Feb 2021.

Vancouver:

Oncescu V. Development Of Point-Of-Care Devices For Rapid Diagnostics And Preventive Care. [Internet] [Doctoral dissertation]. Cornell University; 2014. [cited 2021 Feb 25]. Available from: http://hdl.handle.net/1813/36188.

Council of Science Editors:

Oncescu V. Development Of Point-Of-Care Devices For Rapid Diagnostics And Preventive Care. [Doctoral Dissertation]. Cornell University; 2014. Available from: http://hdl.handle.net/1813/36188

Vanderbilt University

28. Lubbers, Brad Ryan. Nano-Calorimetry for Point of Care Diagnostics.

Degree: PhD, Biomedical Engineering, 2015, Vanderbilt University

URL: http://hdl.handle.net/1803/10713

► Calorimetry has many applications in the physical and life sciences including measuring phase changes, determining reaction equilibria, detecting protein binding events, and quantifying enzyme kinetics.… (more)

Subjects/Keywords: microfabrication; Phenylketonuria; trastuzumab; Thermometric ELISA; nano-calorimetry; point of care

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Lubbers, B. R. (2015). Nano-Calorimetry for Point of Care Diagnostics. (Doctoral Dissertation). Vanderbilt University. Retrieved from http://hdl.handle.net/1803/10713

Chicago Manual of Style (16^th Edition):

Lubbers, Brad Ryan. “Nano-Calorimetry for Point of Care Diagnostics.” 2015. Doctoral Dissertation, Vanderbilt University. Accessed February 25, 2021. http://hdl.handle.net/1803/10713.

MLA Handbook (7^th Edition):

Lubbers, Brad Ryan. “Nano-Calorimetry for Point of Care Diagnostics.” 2015. Web. 25 Feb 2021.

Vancouver:

Lubbers BR. Nano-Calorimetry for Point of Care Diagnostics. [Internet] [Doctoral dissertation]. Vanderbilt University; 2015. [cited 2021 Feb 25]. Available from: http://hdl.handle.net/1803/10713.

Council of Science Editors:

Lubbers BR. Nano-Calorimetry for Point of Care Diagnostics. [Doctoral Dissertation]. Vanderbilt University; 2015. Available from: http://hdl.handle.net/1803/10713

Vanderbilt University

29. Euliano, Erin Marie. Adaptive Multiplexed RT-PCR Assay for Detection of Chikungunya, Dengue, and Zika Viruses.

Degree: MS, Biomedical Engineering, 2018, Vanderbilt University

URL: http://hdl.handle.net/1803/15382

► As chikungunya, dengue, and Zika virus infections all present similar symptoms, a multiplexed assay could provide enhanced diagnostic power to clinicians at the point-of-care in… (more)

Subjects/Keywords: Adaptive PCR; RT-PCR; Zika; dengue; chikungunya; point-of-care diagnostics

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APA (6^th Edition):

Euliano, E. M. (2018). Adaptive Multiplexed RT-PCR Assay for Detection of Chikungunya, Dengue, and Zika Viruses. (Thesis). Vanderbilt University. Retrieved from http://hdl.handle.net/1803/15382

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Euliano, Erin Marie. “Adaptive Multiplexed RT-PCR Assay for Detection of Chikungunya, Dengue, and Zika Viruses.” 2018. Thesis, Vanderbilt University. Accessed February 25, 2021. http://hdl.handle.net/1803/15382.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Euliano, Erin Marie. “Adaptive Multiplexed RT-PCR Assay for Detection of Chikungunya, Dengue, and Zika Viruses.” 2018. Web. 25 Feb 2021.

Vancouver:

Euliano EM. Adaptive Multiplexed RT-PCR Assay for Detection of Chikungunya, Dengue, and Zika Viruses. [Internet] [Thesis]. Vanderbilt University; 2018. [cited 2021 Feb 25]. Available from: http://hdl.handle.net/1803/15382.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Euliano EM. Adaptive Multiplexed RT-PCR Assay for Detection of Chikungunya, Dengue, and Zika Viruses. [Thesis]. Vanderbilt University; 2018. Available from: http://hdl.handle.net/1803/15382

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

McMaster University

30. Fung, Barnabas. A STUDY OF POROUS ELECTRODES FOR DNA ELECTROCHEMICAL DETECTION AND THE DEVELOPMENT OF A HYBRIDIZATION EFFICIENCY CHARACTERIZATION TECHNIQUE.

Degree: MASc, 2016, McMaster University

URL: http://hdl.handle.net/11375/19496

►

Point-of-care DNA diagnostics for resource-limited settings require high sensitivity and low limits of detection but is constrained by a limitation on the complexity of instrumentation… (more)

Subjects/Keywords: Biosensors; Electrochemical; DNA; rapid-prototyping; point-of-care

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APA (6^th Edition):

Fung, B. (2016). A STUDY OF POROUS ELECTRODES FOR DNA ELECTROCHEMICAL DETECTION AND THE DEVELOPMENT OF A HYBRIDIZATION EFFICIENCY CHARACTERIZATION TECHNIQUE. (Masters Thesis). McMaster University. Retrieved from http://hdl.handle.net/11375/19496

Chicago Manual of Style (16^th Edition):

Fung, Barnabas. “A STUDY OF POROUS ELECTRODES FOR DNA ELECTROCHEMICAL DETECTION AND THE DEVELOPMENT OF A HYBRIDIZATION EFFICIENCY CHARACTERIZATION TECHNIQUE.” 2016. Masters Thesis, McMaster University. Accessed February 25, 2021. http://hdl.handle.net/11375/19496.

MLA Handbook (7^th Edition):

Fung, Barnabas. “A STUDY OF POROUS ELECTRODES FOR DNA ELECTROCHEMICAL DETECTION AND THE DEVELOPMENT OF A HYBRIDIZATION EFFICIENCY CHARACTERIZATION TECHNIQUE.” 2016. Web. 25 Feb 2021.

Vancouver:

Fung B. A STUDY OF POROUS ELECTRODES FOR DNA ELECTROCHEMICAL DETECTION AND THE DEVELOPMENT OF A HYBRIDIZATION EFFICIENCY CHARACTERIZATION TECHNIQUE. [Internet] [Masters thesis]. McMaster University; 2016. [cited 2021 Feb 25]. Available from: http://hdl.handle.net/11375/19496.

Council of Science Editors:

Fung B. A STUDY OF POROUS ELECTRODES FOR DNA ELECTROCHEMICAL DETECTION AND THE DEVELOPMENT OF A HYBRIDIZATION EFFICIENCY CHARACTERIZATION TECHNIQUE. [Masters Thesis]. McMaster University; 2016. Available from: http://hdl.handle.net/11375/19496

Open Access Theses and Dissertations