subject:(Point Mutation)

Université de Neuchâtel

1. Lovis, Leonore. Evaluation of acaricide resistance in the cattle tick, 'Rhipicephalus (Boophilus) microplus', using a new in vitro test and molecular tools.

Degree: 2012, Université de Neuchâtel

URL: http://doc.rero.ch/record/30407

► Rhipicephalus (Boophilus) microplus est une tique des bovins à un hôte qui se trouve dans les zones tropicales et subtropicales. Cet ectoparasite a un impact… (more)

▼ Rhipicephalus (Boophilus) microplus est une tique des bovins à un hôte qui se trouve dans les zones tropicales et subtropicales. Cet ectoparasite a un impact économique très important sur l’élevage bovin dans toutes les régions où il se trouve. Au Brésil, par exemple, les pertes dues à son impact direct et indirect ont été estimées à 2 milliards de dollars US en 2000. Le contrôle des populations de tiques des bovins repose essentiellement sur l’utilisation d’acaricides. L’utilisation intensive de ce moyen de lutte a eu pour conséquence le développement de résistances à la majorité des classes d’acaricides disponibles sur le marché. Un suivi local de la résistance aux acaricides est essentiel afin que les éleveurs puissent recevoir des informations sur les composés auxquels les populations de tiques présentes dans leur établissement sont résistantes et être guidés dans le choix de composés de remplacement à disposition. A une échelle globale, le suivi de la résistance permet d’observer sa progression afin d’essayer de ralentir son développement et d’allonger la durée d’utilisation des composés. Les tests in vitro sont des méthodes très utiles pour détecter la résistance des tiques. La FAO recommande deux d’entre eux, l’un utilisant des larves, nommé Larval Packet test (LPT), l’autre utilisant des adultes, appelé Adult Immersion Test (AIT). Chaque test a ses avantages et ses inconvénients : le LPT est un test laborieux qui prend beaucoup de temps alors que le AIT nécessite une grande quantité de tiques. Ces désavantages limitent le nombre de composés et de doses pouvant être testés, limitant ainsi l’information obtenue. Pour surmonter ces difficultés, nous avons développé un nouveau test, nommé Larval Tarsal Test (LTT), qui permet de tester de nombreux composés en peu de temps et avec un minimum de tiques. Dans ce test, des œufs de tiques sont distribués dans des puits de plaques de microtritation préalablement traités avec les acaricides voulant être testés. Les œufs sont incubés jusqu’à l’éclosion des larves, qui sont ainsi exposées aux composés. La résistance des souches de tiques est alors évaluée en fonction de la mortalité des larves écloses. La capacité du LTT à détecter la résistance a été comparée au LPT, un des tests recommandés par la FAO. Pour cela, une souche de tiques sensible ainsi qu’une souche résistante de référence ont été exposées à neuf composés de cinq classes principales d’acaricides : les organophosphorés (OP), les pyréthroïdes de synthèse (SP), les amidines, les lactones macrocycliques (ML) et les phénylpyrazoles. Le LTT a permis d’obtenir de bonnes courbes de dose-réponse, il s’est montré aussi sensible que le LPT et, à mortalités équivalentes, a nécessité des doses de composés nettement plus basses que le LPT. Ayant démontré que le LTT est un test adéquat, des populations de tiques provenant d’Argentine, d’Afrique du Sud et d’Australie ont été envoyées en Suisse pour évaluer leur résistance à l’aide du LTT. Ces tests ont confirmé l’intérêt du LTT à être utilisé pour… Advisors/Committee Members: Bruno (Dir.), Heinz (Codir.).

Subjects/Keywords: point mutation

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APA (6^th Edition):

Lovis, L. (2012). Evaluation of acaricide resistance in the cattle tick, 'Rhipicephalus (Boophilus) microplus', using a new in vitro test and molecular tools. (Thesis). Université de Neuchâtel. Retrieved from http://doc.rero.ch/record/30407

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Lovis, Leonore. “Evaluation of acaricide resistance in the cattle tick, 'Rhipicephalus (Boophilus) microplus', using a new in vitro test and molecular tools.” 2012. Thesis, Université de Neuchâtel. Accessed December 12, 2019. http://doc.rero.ch/record/30407.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Lovis, Leonore. “Evaluation of acaricide resistance in the cattle tick, 'Rhipicephalus (Boophilus) microplus', using a new in vitro test and molecular tools.” 2012. Web. 12 Dec 2019.

Vancouver:

Lovis L. Evaluation of acaricide resistance in the cattle tick, 'Rhipicephalus (Boophilus) microplus', using a new in vitro test and molecular tools. [Internet] [Thesis]. Université de Neuchâtel; 2012. [cited 2019 Dec 12]. Available from: http://doc.rero.ch/record/30407.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Lovis L. Evaluation of acaricide resistance in the cattle tick, 'Rhipicephalus (Boophilus) microplus', using a new in vitro test and molecular tools. [Thesis]. Université de Neuchâtel; 2012. Available from: http://doc.rero.ch/record/30407

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Jawaharlal Nehru University

2. Singh, Parmit Kumar. Genetic and molecular analysis of neurospora duplications and duplication-generating translocations.

Degree: 2010, Jawaharlal Nehru University

URL: http://shodhganga.inflibnet.ac.in/handle/10603/68

► In Neurospora, duplicated DNA sequences are substrates for repeat-induced point mutation (RIP), and my studies show that the RIP machinery is titrated out by >… (more)

▼ In Neurospora, duplicated DNA sequences are substrates for repeat-induced point mutation (RIP), and my studies show that the RIP machinery is titrated out by > 300 kbp of duplicated DNA. RIP is a genome defense process which occurs during the premeiosis of a sexual cross and induces G: C to A: T mutations and cytosine methylation in duplicated DNA in an otherwise haploid genome. Both large (i.e., > 100 kbp) and small duplications (of the order of 0.5 - 5 kbp) are substrates for RIP. Strains bearing a large duplication (Dp strains) can be obtained from a cross of normal sequence strains (N) with some translocation strains. Dp segregants are identifiable by the barren phenotype of Dp x N crosses. That is, Dp x N crosses make normal-looking perithecia but produce very few ascospores. The barrenness is due to an RNAi-based process called meiotic silencing by unpaired DNA (MSUD). The semi-dominant Sad-1, Sad-2 and Sms-2 suppressors of MSUD suppress the barren phenotype of Dp-heterozygous crosses and can increase their productivity.Previous work from our laboratory revealed that five of six large Dp’s could dominantly suppress RIP in a small gene-sized probe duplication called Dp(erg-3). The suppressor Dp’s were all > 270 kbp, whereas the one non-suppressor Dp, Dp(B362i), was approximately 117 kbp. I screened another 35 Dp’s and found that 29 were suppressors and four non-suppressors. For 27 of the 34 suppressor Dp’s I estimated their minimum size to be > 270 kb, and for three non-suppressors Dp’s I estimated the maximum size to be < 200 kb. The size of the remaining Dp’s was not estimated because no covered markers were known for them. I also found that RIP is suppressed in a significant proportion of crosses that were multiply heterozygous for more than one non-suppressor Dp if the combined size of the duplicated DNA was close to or more than 300 kbp. These findings support our hypothesis that large Dp’s suppress RIP via titration of the RIP machinery and the “equivalence point” is ~ 300 kbp. I describe this work in Chapter 3.Breakpoint junction sequences can provide information regarding role of homologous and non-homologous end joining DNA repair pathways in the origin of rearrangements and thus give an idea of the mechanism of mutagenesis. Segment spanning breakpoints have been cloned from various rearrangements of Neurospora, but no nucleotide sequence information was available except for one reciprocal translocation T(IR;VIR)UK-T12. In Chapter 4, I describe further breakpoint localization of 24 Dp’s by analysis of RFLPs coverage and the determination of breakpoint junction sequences (i.e., deletion junctions on the donor and insertion junctions in the recipient LG) of 11translocations by performing inverse PCR with translocation DNA template and primers annealing just “outside” and “within” the translocated segments. Chromosomal rearrangements are known not only to mutate genes by disruption but also known to create gene fusions, which might generate novel chimeric proteins. I analyzed 30 breakpoint junction… Advisors/Committee Members: Kasbekar, D P.

Subjects/Keywords: Neurospora; Duplication; Duplication-generating translocations; Repeat-induced point mutation

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APA (6^th Edition):

Singh, P. K. (2010). Genetic and molecular analysis of neurospora duplications and duplication-generating translocations. (Thesis). Jawaharlal Nehru University. Retrieved from http://shodhganga.inflibnet.ac.in/handle/10603/68

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Singh, Parmit Kumar. “Genetic and molecular analysis of neurospora duplications and duplication-generating translocations.” 2010. Thesis, Jawaharlal Nehru University. Accessed December 12, 2019. http://shodhganga.inflibnet.ac.in/handle/10603/68.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Singh, Parmit Kumar. “Genetic and molecular analysis of neurospora duplications and duplication-generating translocations.” 2010. Web. 12 Dec 2019.

Vancouver:

Singh PK. Genetic and molecular analysis of neurospora duplications and duplication-generating translocations. [Internet] [Thesis]. Jawaharlal Nehru University; 2010. [cited 2019 Dec 12]. Available from: http://shodhganga.inflibnet.ac.in/handle/10603/68.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Singh PK. Genetic and molecular analysis of neurospora duplications and duplication-generating translocations. [Thesis]. Jawaharlal Nehru University; 2010. Available from: http://shodhganga.inflibnet.ac.in/handle/10603/68

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Oregon State University

3. Pomraning, Kyle R. Characterization of Neurospora crassa and Fusarium graminearum mutants defective in repeat-induced point mutation.

Degree: PhD, Molecular and Cellular Biology, 2012, Oregon State University

URL: http://hdl.handle.net/1957/35582

► Mutation of repetitive DNA by repeat-induced point mutation (RIP) is a process that occurs in many filamentous fungi of the Ascomycota during the sexual cycle.… (more)

▼ Mutation of repetitive DNA by repeat-induced point mutation (RIP) is a process that occurs in many filamentous fungi of the Ascomycota during the sexual cycle. Concurrently, direct DNA repeats are often deleted by homologous recombination at high frequency during the sexual cycle. Thus, the processes of RIP and deletion compete to either mutate or remove repetitive DNA from the genome of filamentous fungi during sexual cycles. Both processes contribute to genome streamlining by controlling proliferation of transposable elements and by limiting expansion of gene families. While the genetic requirements for deletion by homologous recombination are well known, the mechanism behind the specific detection and mutation of repetitive DNA by RIP has yet to be elucidated as only a single gene essential for RIP, rid, has been identified. We have developed Fusarium graminearum as a model organism for the study of RIP by showing that it mutates repetitive DNA frequently during the sexual cycle and that the mutations due to RIP are dependent on rid. Further, we have sequenced a genetic mapping strain of F. graminearum (00-676-2) and identified 62,310 single nucleotide polymorphisms (SNPs) compared to the reference strain (PH-1). The SNP map will be useful for quickly mapping new mutants by bulk segregant analysis and high-throughput sequencing for which bioinformatic tools were specifically developed. The groundwork has thus been laid for identification of novel RIP mutants in F. graminearum, which being homothallic has a major advantage for identification of recessive mutations. We used a forward genetics approach to shed light on the mechanism of RIP in Neurospora crassa. Two rrr mutants that dominantly r̲educe R̲IP and r̲ecombination were characterized and identified as different mutated alleles of the same gene, rrr-1[superscript L496P] and rrr-1[superscript G325N] by bulk segregant analysis and high-throughput sequencing. Bioinformatic characterization suggests RRR-1 belongs to a previously uncharacterized group of dynamin-like proteins, which are generally involved in membrane fission and fusion. RRR-1-GFP localizes to the nuclear membrane, but not DNA, suggesting it affects RIP and recombination frequency indirectly by altering nuclear membrane dynamics during sexual development and thereby altering temporal aspects of RIP and recombination. We used a reverse genetics approach to determine whether high frequency RIP and homologous recombination of repetitive DNA during the sexual cycle are linked mechanistically or spatio-temporally. We tested strains where genes important for deletion by homologous recombination were knocked out and found all to be completely RIP competent except mre11, which, while sterile in homozygous deletion crosses, displayed lower RIP frequency in heterozygous crosses. This suggests that mre11 has roles in homologous recombination as well as non-homologous end joining may be important for RIP. Collectively, this work developed methods for efficiently mapping mutations and identified a novel… Advisors/Committee Members: Freitag, Michael (advisor), Strauss, Steven (committee member).

Subjects/Keywords: repeat-induced point mutation; Neurospora crassa – Molecular genetics

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Pomraning, K. R. (2012). Characterization of Neurospora crassa and Fusarium graminearum mutants defective in repeat-induced point mutation. (Doctoral Dissertation). Oregon State University. Retrieved from http://hdl.handle.net/1957/35582

Chicago Manual of Style (16^th Edition):

Pomraning, Kyle R. “Characterization of Neurospora crassa and Fusarium graminearum mutants defective in repeat-induced point mutation.” 2012. Doctoral Dissertation, Oregon State University. Accessed December 12, 2019. http://hdl.handle.net/1957/35582.

MLA Handbook (7^th Edition):

Pomraning, Kyle R. “Characterization of Neurospora crassa and Fusarium graminearum mutants defective in repeat-induced point mutation.” 2012. Web. 12 Dec 2019.

Vancouver:

Pomraning KR. Characterization of Neurospora crassa and Fusarium graminearum mutants defective in repeat-induced point mutation. [Internet] [Doctoral dissertation]. Oregon State University; 2012. [cited 2019 Dec 12]. Available from: http://hdl.handle.net/1957/35582.

Council of Science Editors:

Pomraning KR. Characterization of Neurospora crassa and Fusarium graminearum mutants defective in repeat-induced point mutation. [Doctoral Dissertation]. Oregon State University; 2012. Available from: http://hdl.handle.net/1957/35582

University of Guelph

4. Ji, Ae-Ri. An ENU-induced Point Mutation on Chromosome Eight Causes a Nephronophthisis-like Kidney Phenotype .

Degree: 2015, University of Guelph

URL: https://atrium.lib.uoguelph.ca/xmlui/handle/10214/9206

► The forward genetics ENU mutagenesis and phenotyping program at MRC Harwell is designed to produce and screen recessive mutant mouse pedigrees. We examined the histopathology… (more)

▼ The forward genetics ENU mutagenesis and phenotyping program at MRC Harwell is designed to produce and screen recessive mutant mouse pedigrees. We examined the histopathology of six selected pedigrees based on their clinical phenotypes. Homozygotes from pedigree MPC232 showed growth retardation, smaller organ size, increased serum BUN and creatinine, and renal dysplasia and hepatic oval cell/biliary hyperplasia as determined by histopathology. The phenotype was consistent and robust at 4-5 weeks of age leading to renal failure. The renal histopathology was characterized by tubular ectasia at the corticomedullary junction and increased interstitial mesenchyme. Considering the renal phenotype, a defect of mesenchymal-epithelial transition (MET) during renal development or a cystic kidney disease was suspected. An embryonic and early postnatal development study confirmed no developmental defect between embryonic day 15.5 and postnatal day 11. In kidneys of homozygous mice at 4-5 weeks of age, ciliary defect in tubules, epithelial-mesenchymal transition in some tubules, interstitial fibrosis, and interstitial infiltrates of inflammatory cells was verified and validated by Masson’s trichrome staining, immunoblotting, and immunohistochemistry. The temporal and histological features of this renal dysplasia resemble human nephronophthisis. Genetic analysis was performed to identify the causative mutations. Candidate mutations were narrowed down to 4 loci on chromosome 8 using linkage analysis, Illumina genome sequencing, and comparative genomic analysis of G3 littermate mice. Additional genetic investigation using Sanger sequencing and SNaPshot (single nucleotide polymorphism genotyping) further narrowed the candidate mutations down to two A to T mutations, at 8:104324177 (intron variant of Cmtm2b) and at 8:105912712 (missense variant of Pskh1). Little is known about either of these genes. However, a mutation in Pskh1 was recently found in mice with ciliopathies, and a protein interaction between PSKH1 and CDC6 has been proposed in humans. MPC232 homozygous mice showed a significant decrease in PSKH1 and CDC6 expression, and an increase in E-cadherin and β-catenin. Further, augmented cytoplasm-only β-catenin suggested that disrupted β-catenin-related pathways mechanistically underlie the phenotype of the MPC232 pedigree. In summary, we characterized a mouse model of nephronophthisis and we propose a potential novel role of Pskh1 in its pathogenesis. Advisors/Committee Members: Wood, Geoffrey (advisor), McKerlie, Colin (advisor).

Subjects/Keywords: ciliopathy; Pskh1; Cmtm2b; nephronophthisis; cystic kidney disease; ENU; point mutation

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Ji, A. (2015). An ENU-induced Point Mutation on Chromosome Eight Causes a Nephronophthisis-like Kidney Phenotype . (Thesis). University of Guelph. Retrieved from https://atrium.lib.uoguelph.ca/xmlui/handle/10214/9206

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Ji, Ae-Ri. “An ENU-induced Point Mutation on Chromosome Eight Causes a Nephronophthisis-like Kidney Phenotype .” 2015. Thesis, University of Guelph. Accessed December 12, 2019. https://atrium.lib.uoguelph.ca/xmlui/handle/10214/9206.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Ji, Ae-Ri. “An ENU-induced Point Mutation on Chromosome Eight Causes a Nephronophthisis-like Kidney Phenotype .” 2015. Web. 12 Dec 2019.

Vancouver:

Ji A. An ENU-induced Point Mutation on Chromosome Eight Causes a Nephronophthisis-like Kidney Phenotype . [Internet] [Thesis]. University of Guelph; 2015. [cited 2019 Dec 12]. Available from: https://atrium.lib.uoguelph.ca/xmlui/handle/10214/9206.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Ji A. An ENU-induced Point Mutation on Chromosome Eight Causes a Nephronophthisis-like Kidney Phenotype . [Thesis]. University of Guelph; 2015. Available from: https://atrium.lib.uoguelph.ca/xmlui/handle/10214/9206

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

5. Hietpas, Ryan T. Experimental Illumination of Comprehensive Fitness Landscapes: A Dissertation.

Degree: Biochemistry and Molecular Pharmacology, Biochemistry and Molecular Pharmacology, 2013, U of Massachusetts : Med

URL: http://escholarship.umassmed.edu/gsbs_diss/667

► Evolution is the single cohesive logical framework in which all biological processes may exist simultaneously. Incremental changes in phenotype over imperceptibly large timescales have… (more)

▼ Evolution is the single cohesive logical framework in which all biological processes may exist simultaneously. Incremental changes in phenotype over imperceptibly large timescales have given rise to the enormous diversity of life we witness on earth both presently and through the natural record. The basic unit of evolution is mutation, and by perturbing biological processes, mutations may alter the fitness of an individual. However, the fitness effect of a mutation is difficult to infer from historical record, and complex to obtain experimentally in an efficient and accurate manner. We have recently developed a high throughput method to iteratively mutagenize regions of essential genes in yeast and subsequently analyze individual mutant fitness termed Exceedingly Methodical and Parallel Investigation of Randomized Individual Codons (EMPIRIC). Utilizing this technique as exemplified in Chapters II and III, it is possible to determine the fitness effects of all possible point mutations in parallel through growth competition followed by a high throughput sequencing readout. We have employed this technique to determine the distribution of fitness effects in a nine amino acid region of the Hsp90 gene of S. cerevisiae under elevated temperature, and found the bimodal distribution of fitness effects to be remarkably consistent with near-neutral theory. Comparing the measured fitness effects of mutants to the natural record, phylogenetic alignments appear to be a poor predictor of experimental fitness. In Chapter IV, to further interrogate the properties of this region, library competition under conditions of elevated temperature and salinity were performed to study the potential of protein adaptation. Strikingly, whereas both optimal and elevated temperatures produced no statistically significant beneficial mutations, under conditions of elevated salinity, adaptive mutations appear with fitness advantages up to 8% greater than wild type. Of particular interest, mutations conferring fitness benefits under conditions of elevated salinity almost always experience a fitness defect in other experimental conditions, indicating these mutations are environmentally specialized. Applying the experimental fitness measurements to long standing theoretical predictions of adaptation, our results are remarkably consistent with Fisher’s Geometric Model of protein evolution. Epistasis between mutations can have profound effects on evolutionary trajectories. Although the importance of epistasis has been realized since the early 1900s, the interdependence of mutations is difficult to study in vivo due to the stochastic and constant nature of background mutations. In Chapter V, utilizing the EMPIRIC methodology allows us to study the distribution of fitness effects in the context of mutant genetic backgrounds with minimal influence from unintended background mutations. By analyzing intragenic epistatic interactions, we uncovered a complex interplay between solvent shielded structural residues and… Advisors/Committee Members: Daniel N. A. Bolon, PhD.

Subjects/Keywords: Molecular Evolution; Mutation; Genetic Fitness; Genetic Techniques; Point Mutation; Saccharomyces cerevisiae Proteins; Computational Biology; Evolution; Molecular Genetics

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Hietpas, R. T. (2013). Experimental Illumination of Comprehensive Fitness Landscapes: A Dissertation. (Doctoral Dissertation). U of Massachusetts : Med. Retrieved from http://escholarship.umassmed.edu/gsbs_diss/667

Chicago Manual of Style (16^th Edition):

Hietpas, Ryan T. “Experimental Illumination of Comprehensive Fitness Landscapes: A Dissertation.” 2013. Doctoral Dissertation, U of Massachusetts : Med. Accessed December 12, 2019. http://escholarship.umassmed.edu/gsbs_diss/667.

MLA Handbook (7^th Edition):

Hietpas, Ryan T. “Experimental Illumination of Comprehensive Fitness Landscapes: A Dissertation.” 2013. Web. 12 Dec 2019.

Vancouver:

Hietpas RT. Experimental Illumination of Comprehensive Fitness Landscapes: A Dissertation. [Internet] [Doctoral dissertation]. U of Massachusetts : Med; 2013. [cited 2019 Dec 12]. Available from: http://escholarship.umassmed.edu/gsbs_diss/667.

Council of Science Editors:

Hietpas RT. Experimental Illumination of Comprehensive Fitness Landscapes: A Dissertation. [Doctoral Dissertation]. U of Massachusetts : Med; 2013. Available from: http://escholarship.umassmed.edu/gsbs_diss/667

6. QIAN ZHUOLEI. Molecular analysis of mutations in Agrobacterium tumefaciens under selection pressure.

Degree: 2008, National University of Singapore

URL: http://scholarbank.nus.edu.sg/handle/10635/13080

Subjects/Keywords: Agrobacterium point mutation insertion mutation two-component system tretracycline

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

ZHUOLEI, Q. (2008). Molecular analysis of mutations in Agrobacterium tumefaciens under selection pressure. (Thesis). National University of Singapore. Retrieved from http://scholarbank.nus.edu.sg/handle/10635/13080

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

ZHUOLEI, QIAN. “Molecular analysis of mutations in Agrobacterium tumefaciens under selection pressure.” 2008. Thesis, National University of Singapore. Accessed December 12, 2019. http://scholarbank.nus.edu.sg/handle/10635/13080.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

ZHUOLEI, QIAN. “Molecular analysis of mutations in Agrobacterium tumefaciens under selection pressure.” 2008. Web. 12 Dec 2019.

Vancouver:

ZHUOLEI Q. Molecular analysis of mutations in Agrobacterium tumefaciens under selection pressure. [Internet] [Thesis]. National University of Singapore; 2008. [cited 2019 Dec 12]. Available from: http://scholarbank.nus.edu.sg/handle/10635/13080.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

ZHUOLEI Q. Molecular analysis of mutations in Agrobacterium tumefaciens under selection pressure. [Thesis]. National University of Singapore; 2008. Available from: http://scholarbank.nus.edu.sg/handle/10635/13080

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Florida

7. Carver, Ryan. A search for genes related to salicylic acid, a signaling molecule, in Arabidopsis thaliana.

Degree: 2010, University of Florida

URL: http://ufdc.ufl.edu/AA00060591

► Salicylic Acid (SA) is an important immunological signaling molecule for plants when stressed with a biotropic pathogens7,8. SA accumulation and production are associated with the… (more)

Subjects/Keywords: Biosensing techniques; DNA; Enzymes; Genes; Genetic mutation; Genomes; Pathogens; Phenotypes; Point mutation; Seeds; Arabidopsis thaliana; Cloning; Salicylic acid

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APA (6^th Edition):

Carver, R. (2010). A search for genes related to salicylic acid, a signaling molecule, in Arabidopsis thaliana. (Thesis). University of Florida. Retrieved from http://ufdc.ufl.edu/AA00060591

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Carver, Ryan. “A search for genes related to salicylic acid, a signaling molecule, in Arabidopsis thaliana.” 2010. Thesis, University of Florida. Accessed December 12, 2019. http://ufdc.ufl.edu/AA00060591.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Carver, Ryan. “A search for genes related to salicylic acid, a signaling molecule, in Arabidopsis thaliana.” 2010. Web. 12 Dec 2019.

Vancouver:

Carver R. A search for genes related to salicylic acid, a signaling molecule, in Arabidopsis thaliana. [Internet] [Thesis]. University of Florida; 2010. [cited 2019 Dec 12]. Available from: http://ufdc.ufl.edu/AA00060591.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Carver R. A search for genes related to salicylic acid, a signaling molecule, in Arabidopsis thaliana. [Thesis]. University of Florida; 2010. Available from: http://ufdc.ufl.edu/AA00060591

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Florida

8. Roberts, Brittany-Lee. Effect of Charge and Polarity Changes on the Propensity of Inclusion Formation by Mutant SOD1.

Degree: 2013, University of Florida

URL: http://ufdc.ufl.edu/AA00060949

► The present study investigated the relationship between changes in charge and polarity on the propensity of aggregate formation by mutant SOD1. Two different mutation sites… (more)

Subjects/Keywords: Aggregation; Amyotrophic lateral sclerosis; Cell aggregates; Charge separation; Genetic mutation; Inclusion bodies; Motor neurons; Plasmids; Point mutation; Superoxides; Amino acids; Mutation (Biology); Polarity (Biology); Superoxide dismutase

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APA (6^th Edition):

Roberts, B. (2013). Effect of Charge and Polarity Changes on the Propensity of Inclusion Formation by Mutant SOD1. (Thesis). University of Florida. Retrieved from http://ufdc.ufl.edu/AA00060949

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Roberts, Brittany-Lee. “Effect of Charge and Polarity Changes on the Propensity of Inclusion Formation by Mutant SOD1.” 2013. Thesis, University of Florida. Accessed December 12, 2019. http://ufdc.ufl.edu/AA00060949.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Roberts, Brittany-Lee. “Effect of Charge and Polarity Changes on the Propensity of Inclusion Formation by Mutant SOD1.” 2013. Web. 12 Dec 2019.

Vancouver:

Roberts B. Effect of Charge and Polarity Changes on the Propensity of Inclusion Formation by Mutant SOD1. [Internet] [Thesis]. University of Florida; 2013. [cited 2019 Dec 12]. Available from: http://ufdc.ufl.edu/AA00060949.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Roberts B. Effect of Charge and Polarity Changes on the Propensity of Inclusion Formation by Mutant SOD1. [Thesis]. University of Florida; 2013. Available from: http://ufdc.ufl.edu/AA00060949

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

9. Ramet, Lauriane. Caractérisation d'une mutation humaine du transporteur vésiculaire du glutamate de type 3 (VGLUT3) : VGLUT3-p.A211V dans le système nerveux central de souris : Characterization of a human mutation of vesicular glutamate transporter type three (VGLUT3) : VGLUT3-p.A211V in mouse central nervous system.

Degree: Docteur es, Neurosciences, 2015, Université Pierre et Marie Curie – Paris VI

URL: http://www.theses.fr/2015PA066700

►

Le glutamate est accumulé dans des vésicules synaptiques par des transporteurs vésiculaires du glutamate appelés VGLUT1-3. VGLUT1 et VGLUT2 sont utilisés par les neurones glutamatergiques… (more)

▼

Le glutamate est accumulé dans des vésicules synaptiques par des transporteurs vésiculaires du glutamate appelés VGLUT1-3. VGLUT1 et VGLUT2 sont utilisés par les neurones glutamatergiques «classiques» corticaux et sous-corticaux. VGLUT3 est présent dans des sous-populations de neurones utilisant d’autres neurotransmetteurs que le glutamate. Dans la cochlée, VGLUT3 permet la transmission glutamatergique entre les cellules ciliées internes et les neurones du nerf auditif. Le travail mené par l’équipe du Pr Puel a permis de découvrir l’implication de VGLUT3 dans une pathologie héréditaire de l’audition chez l’Homme. Une mutation p.A211V du gène codant VGLUT3 humain est responsable d’une surdité progressive à transmission autosomique. Il s’agit de la première mutation d’un VGLUT associé à une pathologie humaine. Mon travail de thèse a consisté à caractériser l’impact de cette mutation sur le SNC d’une lignée de souris exprimant cette mutation. Nous avons observé que cette mutation avait des effets complexes sur VGLUT3. La mutation p.A211V entraine une baisse marquée de l’expression de VGLUT3 dans les terminaisons nerveuses qui semble liée à une dégradation accélérée de VGLUT3. 20% d’expression résiduelle de VGLUT3 suffisent à assurer la majeure partie des fonctions du transporteur. L’activité de VGLUT3 ne semble donc pas être linéairement corrélée à son expression. De plus, la réduction de VGLUT3 au niveau des synapses semble s’accompagner d’une réduction du nombre de vésicules VGLUT3-positives et d’une réduction du nombre de copies de VGLUT3 par vésicule. Dans l’ensemble, mon travail de thèse a permis d’acquérir une meilleure connaissance de la régulation de VGLUT3.

Glutamate is the major excitatory neurotransmitter in the Central Nervous System (CNS) and is accumulated into synaptic vesicles by proton-dependent transporters named VGLUT1-3. VGLUT1 and VGLUT2-positive neurons are respectively found in cortical and subcortical glutamatergic neurons. In contrast, VGLUT3 is localized in a small population of neurons using other neurotransmitter than glutamate i.e.: cholinergic interneurons in the striatum, subpopulation of GABAergic interneurons in the hippocampus and cortex and serotoninergic neurons. Furthermore, VGLUT3 is also expressed by sensory inner hear cells (IHCs).In the cochlea, VGLUT3 accumulates glutamate into synaptic vesicles of the IHCs. A mutation of the gene that encodes VGLUT3 is responsible for a progressive, high-frequency deafness. It is the first mutation of a VGLUT that was demonstrated to be responsible for a human pathology.We investigated the effects of the p.A211V mutation on VGLUT3 in the CNS of a mouse line expressing this mutation. We observed that this mutation had complex effects on VGLUT3. The p.A211V mutation causes a 80% decrease of VGLUT3 in nerve endings. 20% residual expression of VGLUT3 is sufficient to fulfill most part of its functions. Contrary to prevailing views, VGLUT3 global activity is not linearly correlated to VGLUT3 quantity. Futhermore, VGLUT3 reduction seems to be…

Advisors/Committee Members: El Mestikawy, Salah (thesis director).

Subjects/Keywords: Transporteur vésiculaire du glutamate de type 3 (VGLUT3); Mutation ponctuelle; P.a211v; Système nerveux central; Synergie vésiculaire; STED; Vesicular glutamate transporter; Central Nervous System (CNS); Point mutation; 573.86

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Ramet, L. (2015). Caractérisation d'une mutation humaine du transporteur vésiculaire du glutamate de type 3 (VGLUT3) : VGLUT3-p.A211V dans le système nerveux central de souris : Characterization of a human mutation of vesicular glutamate transporter type three (VGLUT3) : VGLUT3-p.A211V in mouse central nervous system. (Doctoral Dissertation). Université Pierre et Marie Curie – Paris VI. Retrieved from http://www.theses.fr/2015PA066700

Chicago Manual of Style (16^th Edition):

Ramet, Lauriane. “Caractérisation d'une mutation humaine du transporteur vésiculaire du glutamate de type 3 (VGLUT3) : VGLUT3-p.A211V dans le système nerveux central de souris : Characterization of a human mutation of vesicular glutamate transporter type three (VGLUT3) : VGLUT3-p.A211V in mouse central nervous system.” 2015. Doctoral Dissertation, Université Pierre et Marie Curie – Paris VI. Accessed December 12, 2019. http://www.theses.fr/2015PA066700.

MLA Handbook (7^th Edition):

Ramet, Lauriane. “Caractérisation d'une mutation humaine du transporteur vésiculaire du glutamate de type 3 (VGLUT3) : VGLUT3-p.A211V dans le système nerveux central de souris : Characterization of a human mutation of vesicular glutamate transporter type three (VGLUT3) : VGLUT3-p.A211V in mouse central nervous system.” 2015. Web. 12 Dec 2019.

Vancouver:

Ramet L. Caractérisation d'une mutation humaine du transporteur vésiculaire du glutamate de type 3 (VGLUT3) : VGLUT3-p.A211V dans le système nerveux central de souris : Characterization of a human mutation of vesicular glutamate transporter type three (VGLUT3) : VGLUT3-p.A211V in mouse central nervous system. [Internet] [Doctoral dissertation]. Université Pierre et Marie Curie – Paris VI; 2015. [cited 2019 Dec 12]. Available from: http://www.theses.fr/2015PA066700.

Council of Science Editors:

Ramet L. Caractérisation d'une mutation humaine du transporteur vésiculaire du glutamate de type 3 (VGLUT3) : VGLUT3-p.A211V dans le système nerveux central de souris : Characterization of a human mutation of vesicular glutamate transporter type three (VGLUT3) : VGLUT3-p.A211V in mouse central nervous system. [Doctoral Dissertation]. Université Pierre et Marie Curie – Paris VI; 2015. Available from: http://www.theses.fr/2015PA066700

10. Gallano, Pía. Interplay between DMD point mutations and splicing signals in dystrophinopathy phenotypes.

Degree: 2018, Public Library of Science

URL: http://hdl.handle.net/10486/667027

Subjects/Keywords: Dystrophin; Muscular Dystrophy, Duchenne; Frameshift Mutation; Point Mutation; RNA Splicing; Genotype; Medicina

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Gallano, P. (2018). Interplay between DMD point mutations and splicing signals in dystrophinopathy phenotypes. (Thesis). Public Library of Science. Retrieved from http://hdl.handle.net/10486/667027

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Gallano, Pía. “Interplay between DMD point mutations and splicing signals in dystrophinopathy phenotypes.” 2018. Thesis, Public Library of Science. Accessed December 12, 2019. http://hdl.handle.net/10486/667027.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Gallano, Pía. “Interplay between DMD point mutations and splicing signals in dystrophinopathy phenotypes.” 2018. Web. 12 Dec 2019.

Vancouver:

Gallano P. Interplay between DMD point mutations and splicing signals in dystrophinopathy phenotypes. [Internet] [Thesis]. Public Library of Science; 2018. [cited 2019 Dec 12]. Available from: http://hdl.handle.net/10486/667027.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Gallano P. Interplay between DMD point mutations and splicing signals in dystrophinopathy phenotypes. [Thesis]. Public Library of Science; 2018. Available from: http://hdl.handle.net/10486/667027

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

11. Roscoe, Benjamin P. Analyses of All Possible Point Mutations within a Protein Reveals Relationships between Function and Experimental Fitness: A Dissertation.

Degree: Biochemistry and Molecular Pharmacology, Biochemistry and Molecular Pharmacology, 2014, U of Massachusetts : Med

URL: http://escholarship.umassmed.edu/gsbs_diss/716

► The primary amino acid sequence of a protein governs its specific cellular functions. Since the cracking of the genetic code in the late 1950’s,… (more)

Subjects/Keywords: Amino Acid Sequence; Mutagenesis; Point Mutation; Protein Sequence Analysis; Ubiquitin; Biochemistry; Cellular and Molecular Physiology; Molecular Biology; Molecular Genetics

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Roscoe, B. P. (2014). Analyses of All Possible Point Mutations within a Protein Reveals Relationships between Function and Experimental Fitness: A Dissertation. (Doctoral Dissertation). U of Massachusetts : Med. Retrieved from http://escholarship.umassmed.edu/gsbs_diss/716

Chicago Manual of Style (16^th Edition):

Roscoe, Benjamin P. “Analyses of All Possible Point Mutations within a Protein Reveals Relationships between Function and Experimental Fitness: A Dissertation.” 2014. Doctoral Dissertation, U of Massachusetts : Med. Accessed December 12, 2019. http://escholarship.umassmed.edu/gsbs_diss/716.

MLA Handbook (7^th Edition):

Roscoe, Benjamin P. “Analyses of All Possible Point Mutations within a Protein Reveals Relationships between Function and Experimental Fitness: A Dissertation.” 2014. Web. 12 Dec 2019.

Vancouver:

Roscoe BP. Analyses of All Possible Point Mutations within a Protein Reveals Relationships between Function and Experimental Fitness: A Dissertation. [Internet] [Doctoral dissertation]. U of Massachusetts : Med; 2014. [cited 2019 Dec 12]. Available from: http://escholarship.umassmed.edu/gsbs_diss/716.

Council of Science Editors:

Roscoe BP. Analyses of All Possible Point Mutations within a Protein Reveals Relationships between Function and Experimental Fitness: A Dissertation. [Doctoral Dissertation]. U of Massachusetts : Med; 2014. Available from: http://escholarship.umassmed.edu/gsbs_diss/716

University of California – San Francisco

12. Lee, Lawrence Chet-Lun. Text Mining of Point Mutation Information from Biomedical Literature.

Degree: Biological and Medical Informatics, 2008, University of California – San Francisco

URL: http://www.escholarship.org/uc/item/4vg7n69h

► Text mining is a powerful approach to efficiently identify and extract information from large amounts of text. Its application to biomedical literature promises to allow… (more)

▼ Text mining is a powerful approach to efficiently identify and extract information from large amounts of text. Its application to biomedical literature promises to allow researchers the ability to process hundreds and thousands of articles in a way that was never possible before. This new technology, coupled with the increasing amount of published literature and open access literature sources may usher in a new era of meta-research in which computational algorithms can generate new scientific hypotheses from existing information.Many obstacles, however, stand in the way of truly automated methods for text mining. The difficulty in obtaining full text literature, specialized jargon in scientific research, and the ambiguity of biological entity terms are but a few of the challenges that need to be overcome. That being said, semi-automated text mining methods can still greatly aid researchers by identifying topics of interest, reducing the number of articles to read, and targeting relevant information in articles. The judicious and dedicated use of semi-automated methods has the possibility of having a great impact in efficiently distributing the task of manual reading and processing of scientific literature.Our contribution to semi-automated methods for biomedical text mining center on the identification and extraction of point mutation information. Point mutations are an integral aspect of protein research, as they are the vehicle of diversity and the key to functional changes in proteins. They are also represented in a format that lends itself to text mining and can be referenced to the growing numbers of biological sequence databases. This dissertation focuses on the ability to parse literature for point mutations and extract their functional effects. We show that, using statistical, graph theoretical, and machine learning methods, we can efficiently transform information that was previously embedded in the text into information that is computationally stored and processable.

Subjects/Keywords: Biology, Bioinformatics; Computer Science; point mutation; text mining

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Lee, L. C. (2008). Text Mining of Point Mutation Information from Biomedical Literature. (Thesis). University of California – San Francisco. Retrieved from http://www.escholarship.org/uc/item/4vg7n69h

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Lee, Lawrence Chet-Lun. “Text Mining of Point Mutation Information from Biomedical Literature.” 2008. Thesis, University of California – San Francisco. Accessed December 12, 2019. http://www.escholarship.org/uc/item/4vg7n69h.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Lee, Lawrence Chet-Lun. “Text Mining of Point Mutation Information from Biomedical Literature.” 2008. Web. 12 Dec 2019.

Vancouver:

Lee LC. Text Mining of Point Mutation Information from Biomedical Literature. [Internet] [Thesis]. University of California – San Francisco; 2008. [cited 2019 Dec 12]. Available from: http://www.escholarship.org/uc/item/4vg7n69h.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Lee LC. Text Mining of Point Mutation Information from Biomedical Literature. [Thesis]. University of California – San Francisco; 2008. Available from: http://www.escholarship.org/uc/item/4vg7n69h

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

13. Carlier, Florian. Fonctions et organisations de l’hétérochromatine au cours du développement sexué chez le champignon filamenteux Podospora anserina : Heterochromatin Functions and Organizations during Sexual Development in the Filamentous Fungus Podospora anserina.

Degree: Docteur es, Sciences de la vie et de la santé, 2018, Paris Saclay

URL: http://www.theses.fr/2018SACLS457

► Pour se défendre des effets délétères des éléments transposables, les pezizomycotina ont développé un système de défense génétique et épigénétique appelé « Repeat Induced Point… (more)

▼ Pour se défendre des effets délétères des éléments transposables, les pezizomycotina ont développé un système de défense génétique et épigénétique appelé « Repeat Induced Point Mutation » (RIP). Chez N. crassa, le RIP survient dans la cellule dicaryotique avant la caryogamie et conduit à la méthylation de novo des cytosines (5mC) inclues dans les séquences répétées de chacun des noyaux parentaux haploïdes. De plus, certaines de ces cytosines sont la cible d’un processus de mutation qui les transforme en thymines. Cette étape est suivie par la mise en place locale de l’hétérochromatine constitutive permettant une répression transcriptionnelle durable des séquences cibles du RIP au cours des divisions nucléaires. L’acteur majeur du RIP correspond à une cytosine méthyltransférase putative appelée RID (RIP Defective). Bien que son génome ne montre pas une quantité significative de 5mC, l’inactivation de PaRid chez Podospora anserina aboutit à un blocage du développement sexué survenant après la fécondation. Dans ce contexte, nous avons voulu déterminer si la fonction de PaRid dans le développement sexué consiste à éteindre l’expression de gènes cibles via l’installation de foyers d’hétérochromatine constitutive aux loci concernés. Pour ce faire, nous avons identifié les gènes PaKmt1 et PaHP1, codant respectivement l’histone méthyltransférase PaKmt1 (l’homologue de SU(VAR)39 qui catalyse la tri-méthylation du résidu H3K9 (H3K9me3) et PaHP1 (l’homologue de Heterochromatin Protein 1 qui se lie à H3K9me3). Les deux protéines interviennent dans une même voie de régulation qui aboutit à la mise en place de l’hétérochromatine constitutive. Par opposition, PaKmt6, homologue de l’histone méthyltransférase E(Z), correspond à la sous-unité catalytique du complexe PRC2 qui catalyse la marque H3K27me3 pour permettre l’établissement de l’hétérochromatine facultative. Nos résultats ont montré que l’absence de PaKmt1 et PaHP1 ne provoquent que des défauts mineurs. A l’inverse, l’inactivation du gène PaKmt6 conduit à un ensemble de défauts sévères : croissance végétative altérée, surproduction des gamètes mâles, malformations critiques des fructifications, production très réduite d’ascospores dont la germination est pour partie déficiente. Une étude d’épistasie a montré que les protéines PaRid et PaKmt6 interviennent chacune dans deux voies développementales distinctes. Par ailleurs, nous avons établi par immuno-précipitation de la chromatine les profils de distribution à l’échelle du génome entier des modifications H3K9me3, H3K27me3 et H3K4me3. Caractéristique rare, la marque H3K9me3 colocalise avec H3K27me3 sur des gènes transcriptionnellement réprimés et les séquences répétées ripées. Conformément à sa fonction canonique, H3K4me3 est présente en 5’ des gènes transcrits et est exclue des domaines H3K9me3 et H3K27me3. Comme attendue, PaKmt6 est essentielle à la mise en place de la marque H3K27me3, mais, de manière surprenante, elle serait aussi impliquée dans le dépôt et/ou le maintien d’une partie des marques H3K9me3, dévoilant ainsi… Advisors/Committee Members: Malagnac, Fabienne (thesis director).

Subjects/Keywords: H3K27me3; H3K9me3; H3K4me3; Repeat induced point mutation; Champignons filamenteux; Développement sexué; Hétérochromatine constitutive; Hétérochromatine facultative; Epigénétique; H3K9me3; H3K27me3; H3K4me3; Filamentous fungi; Repeat induced point mutation; Sexual development; Constitutive heterochromatin; Facultative heterochromatin; Epigenetics

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Carlier, F. (2018). Fonctions et organisations de l’hétérochromatine au cours du développement sexué chez le champignon filamenteux Podospora anserina : Heterochromatin Functions and Organizations during Sexual Development in the Filamentous Fungus Podospora anserina. (Doctoral Dissertation). Paris Saclay. Retrieved from http://www.theses.fr/2018SACLS457

Chicago Manual of Style (16^th Edition):

Carlier, Florian. “Fonctions et organisations de l’hétérochromatine au cours du développement sexué chez le champignon filamenteux Podospora anserina : Heterochromatin Functions and Organizations during Sexual Development in the Filamentous Fungus Podospora anserina.” 2018. Doctoral Dissertation, Paris Saclay. Accessed December 12, 2019. http://www.theses.fr/2018SACLS457.

MLA Handbook (7^th Edition):

Carlier, Florian. “Fonctions et organisations de l’hétérochromatine au cours du développement sexué chez le champignon filamenteux Podospora anserina : Heterochromatin Functions and Organizations during Sexual Development in the Filamentous Fungus Podospora anserina.” 2018. Web. 12 Dec 2019.

Vancouver:

Carlier F. Fonctions et organisations de l’hétérochromatine au cours du développement sexué chez le champignon filamenteux Podospora anserina : Heterochromatin Functions and Organizations during Sexual Development in the Filamentous Fungus Podospora anserina. [Internet] [Doctoral dissertation]. Paris Saclay; 2018. [cited 2019 Dec 12]. Available from: http://www.theses.fr/2018SACLS457.

Council of Science Editors:

Carlier F. Fonctions et organisations de l’hétérochromatine au cours du développement sexué chez le champignon filamenteux Podospora anserina : Heterochromatin Functions and Organizations during Sexual Development in the Filamentous Fungus Podospora anserina. [Doctoral Dissertation]. Paris Saclay; 2018. Available from: http://www.theses.fr/2018SACLS457

14. Mariani, Beatriz Marinho de Paula. Padronização das técnicas de PNA e PCR em tempo real para detecção das mutações ativadoras no GNAS na síndrome de McCune-Albright.

Degree: Mestrado, Endocrinologia, 2012, University of São Paulo

URL: http://www.teses.usp.br/teses/disponiveis/5/5135/tde-20122012-105425/ ;

►

A síndrome de McCune Albrigth (SMA) é uma doença genética não hereditária, com incidência estimada entre 1/100.000 e 1/1.000.000 casos/ano. A SMA caracteriza-se clinicamente pela… (more)

▼

A síndrome de McCune Albrigth (SMA) é uma doença genética não hereditária, com incidência estimada entre 1/100.000 e 1/1.000.000 casos/ano. A SMA caracteriza-se clinicamente pela tríade: displasia óssea fibrosa (FD), manchas cutâneas café-com-leite e hiperfunção endócrina tais como: síndrome de Cushing, pseudo-puberdade precoce, hipertiroidismo, acromegalia. O diagnóstico da SMA clássica é usualmente baseado no quadro clínico associado a dosagens hormonais e exames de imagem, principalmente cintilografia do esqueleto. No entanto, quadros atípicos e formas parciais muitas vezes dificultam o diagnóstico preciso da síndrome. O objetivo deste estudo foi padronizar dentre as técnicas de PNA (peptide nucleic acid) e PCT em Tempo Real, para a detecção de polimorfismos de base única (SNPs), a técnica mais sensível para a discriminação das mutações ativadoras da subunidade da proteína G. Para este estudo foram selecionados 32 pacientes, 1 masculino e 31 femininos, com SMA, todos em seguimento no Hospital das Clínicas da Faculdade de Medicina da USP. Como resultado positivo, apresentamos nesse trabalho pela primeira vez o uso do RT-PCR genotipagem na detecção das mutações ativadoras da proteína G, em DNA extraído de tecidos afetados e em leucócitos de sangue periférico, sendo a técnica considerada sensível o suficiente para discriminar de forma simples e rápida as mutações ativadoras da PGs. Sugerimos nesse estudo o uso da técnica de discriminação alélica pelo sistema Taqman. Essa técnica possibilita a detecção destas mutações gsp no sangue periférico mesmo numa baixa porcentagem, uma vez que nem sempre o tecido afetado (gônada, osso, hipófise) é disponível.

The McCune-Albright Syndrome (MAS) is a genetic disease, with incidence estimated at 1/100.000 and 1/1000000 cases per year. MAS is clinically characterized by the triad: bone fibrous dysplasia (FD) café-au-lait skin spots and endocrine hyperfunction, such as: precocious puberty (PP), Cushing's syndrome, hyperthyroidism and acromegaly. The diagnosis of MAS is originally based on clinical characteristics associated with hormonal and imaging studies. However, atypical and partial forms often hamper the accurate diagnosis of the syndrome. For this study we selected 32 patients, 1male and 31 females, all being treated in Hospital das Clínicas, School of Medicine, University of São Paulo. As a positive result, we showed for the first time the use of Real Time PCR/genotyping for the detection of activating mutations of the stimulatory G protein, using blood leucocytes DNA. This technique was sensible and can bring fast results for the patient and the physician, making the diagnosis easier. Our study proposes the use of allelic discrimination by Taqman system, which can be used as a probe that allows the identification of specific genotypes. These techniques could help detect these mutations in peripheral blood when the affected tissue is not available.

Advisors/Committee Members: Fragoso, Maria Candida Barisson Villares.

Subjects/Keywords: Fibrous dysplasia polyostotic; GTP-binding protein alpha subunits Gs; McCune-Albright syndrome; Mutação puntual; Point mutation; Síndrome de McCune-Albright; Subunidades alfa Gs de proteínas de ligação ao GTP

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Mariani, B. M. d. P. (2012). Padronização das técnicas de PNA e PCR em tempo real para detecção das mutações ativadoras no GNAS na síndrome de McCune-Albright. (Masters Thesis). University of São Paulo. Retrieved from http://www.teses.usp.br/teses/disponiveis/5/5135/tde-20122012-105425/ ;

Chicago Manual of Style (16^th Edition):

Mariani, Beatriz Marinho de Paula. “Padronização das técnicas de PNA e PCR em tempo real para detecção das mutações ativadoras no GNAS na síndrome de McCune-Albright.” 2012. Masters Thesis, University of São Paulo. Accessed December 12, 2019. http://www.teses.usp.br/teses/disponiveis/5/5135/tde-20122012-105425/ ;.

MLA Handbook (7^th Edition):

Mariani, Beatriz Marinho de Paula. “Padronização das técnicas de PNA e PCR em tempo real para detecção das mutações ativadoras no GNAS na síndrome de McCune-Albright.” 2012. Web. 12 Dec 2019.

Vancouver:

Mariani BMdP. Padronização das técnicas de PNA e PCR em tempo real para detecção das mutações ativadoras no GNAS na síndrome de McCune-Albright. [Internet] [Masters thesis]. University of São Paulo; 2012. [cited 2019 Dec 12]. Available from: http://www.teses.usp.br/teses/disponiveis/5/5135/tde-20122012-105425/ ;.

Council of Science Editors:

Mariani BMdP. Padronização das técnicas de PNA e PCR em tempo real para detecção das mutações ativadoras no GNAS na síndrome de McCune-Albright. [Masters Thesis]. University of São Paulo; 2012. Available from: http://www.teses.usp.br/teses/disponiveis/5/5135/tde-20122012-105425/ ;

Universidade Federal de Viçosa

15. Raíssa Mesquita Braga. Identificação e caracterização de elementos transponíveis da classe II em Colletotrichum graminicola.

Degree: 2012, Universidade Federal de Viçosa

URL: http://www.tede.ufv.br/tedesimplificado/tde_busca/arquivo.php?codArquivo=3861

► Colletotrichum é um dos gêneros mais importantes de fungos fitopatogênicos em todo o mundo. As espécies fitopatogênicas desse gênero apresentam ciclo de vida hemibiotrófico e… (more)

▼ Colletotrichum é um dos gêneros mais importantes de fungos fitopatogênicos em todo o mundo. As espécies fitopatogênicas desse gênero apresentam ciclo de vida hemibiotrófico e causam doenças em diversas culturas economicamente importantes. Além da importância econômica, Colletotrichum possui grande relevância como um sistema modelo para o estudo das bases celulares e moleculares da patogenicidade fúngica. A espécie Colletotrichum graminicola, agente causal da antracnose do milho (Zea mays), possui ciclo sexual raro e foi a primeira espécie do gênero a ter o seu genoma completamente sequenciado. Os elementos transponíveis são ubíquos e constituem uma fonte de novas mutações, sendo, portanto, uma importante fonte de variabilidade genética. Esses elementos são divididos em duas classes de acordo com a presença ou ausência de um intermediário de RNA na transposição. Os elementos da classe I se transpõem via intermediário de RNA, enquanto os elementos da classe II se transpõem diretamente como DNA. Os elementos transponíveis podem ser utilizados como agentes mutagênicos visando à identificação e etiquetagem de genes e em estudos filogenéticos e populacionais. Tendo em vista a importância dos elementos transponíveis na geração de variabilidade genética e as suas aplicações na pesquisa, o objetivo deste trabalho foi identificar e caracterizar elementos transponíveis da classe II no genoma de C. graminicola. Para tanto, foi utilizada uma abordagem de bioinformática (análises in silico) aliada às atividades experimentais. Foram identificadas 133 sequências completas de elementos transponíveis no genoma sequenciado de C. graminicola, que representam uma proporção relevante do genoma (0,47%). Os elementos foram classificados em 6 famílias de acordo com a identidade e apresentam características da superfamília Tc1-Mariner. Apesar de algumas transposases putativas codificadas por esses elementos possuírem domínio DDE conservado, todas estão interrompidas por vários códons de parada. Nenhum elemento identificado possui todas as características necessárias para um elemento autônomo. A análise in silico revelou evidências de mutações geradas pelo mecanismo de RIP (Mutação de ponto induzida por repetição). O elemento TCg1, amplificado por PCR a partir de um isolado brasileiro de C. graminicola, possui extremidades repetidas invertidas imperfeitas e a sequência putativa da transposase apresenta os três domínios característicos conservados: DDE, HTH e CENPB. Entretanto, essa sequência está interrompida por códons de parada e não foram localizados os códons de iniciação e de terminação, sendo, portanto, provavelmente inativa. O DNA genômico de 49 diferentes isolados foi analisado por hibridização com uma sequência derivada da região interna de TCg1 e apresentaram diferentes perfis. A estratégia utilizada permitiu uma identificação eficiente de uma variedade de elementos transponíveis Tc1-Mariner degenerados por mutações características de RIP em C. graminicola. É improvável que algum dos elementos identificados seja autônomo, entretanto,… Advisors/Committee Members: Marisa Vieira de Queiroz, Elza Fernandes de Araujo, Denise Mara Soares Bazzolli, Sérgio Hermínio Brommonschenkel.

Subjects/Keywords: Transposon; MICROBIOLOGIA AGRICOLA; Transposase; Gênero Colletotrichum; Mutação de ponto induzida por repetição; RIP; Transposon; Transposase; Genus Colletotrichum; Repeat point induced mutation; RIP

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Braga, R. M. (2012). Identificação e caracterização de elementos transponíveis da classe II em Colletotrichum graminicola. (Thesis). Universidade Federal de Viçosa. Retrieved from http://www.tede.ufv.br/tedesimplificado/tde_busca/arquivo.php?codArquivo=3861

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Braga, Raíssa Mesquita. “Identificação e caracterização de elementos transponíveis da classe II em Colletotrichum graminicola.” 2012. Thesis, Universidade Federal de Viçosa. Accessed December 12, 2019. http://www.tede.ufv.br/tedesimplificado/tde_busca/arquivo.php?codArquivo=3861.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Braga, Raíssa Mesquita. “Identificação e caracterização de elementos transponíveis da classe II em Colletotrichum graminicola.” 2012. Web. 12 Dec 2019.

Vancouver:

Braga RM. Identificação e caracterização de elementos transponíveis da classe II em Colletotrichum graminicola. [Internet] [Thesis]. Universidade Federal de Viçosa; 2012. [cited 2019 Dec 12]. Available from: http://www.tede.ufv.br/tedesimplificado/tde_busca/arquivo.php?codArquivo=3861.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Braga RM. Identificação e caracterização de elementos transponíveis da classe II em Colletotrichum graminicola. [Thesis]. Universidade Federal de Viçosa; 2012. Available from: http://www.tede.ufv.br/tedesimplificado/tde_busca/arquivo.php?codArquivo=3861

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Western Ontario

16. Luo, Bin. Statistical tools for assessment of spatial properties of mutations observed under the microarray platform.

Degree: 2018, University of Western Ontario

URL: https://ir.lib.uwo.ca/etd/5746

► Mutations are alterations of the DNA nucleotide sequence of the genome. Analyses of spatial properties of mutations are critical for understanding certain mutational mechanisms relevant… (more)

▼ Mutations are alterations of the DNA nucleotide sequence of the genome. Analyses of spatial properties of mutations are critical for understanding certain mutational mechanisms relevant to genetic disease, diversity, and evolution. The studies in this thesis focus on two types of mutations: point mutations, i.e., single nucleotide polymorphism (SNP) genotype differences, and mutations in segments, i.e., copy number variations (CNVs). The microarray platform, such as the Mouse Diversity Genotyping Array (MDGA), detects these mutations genome-wide with lower cost compared to whole genome sequencing, and thus is considered for suitability as a screening tool for large populations. Yet it provides observation of mutations with high degree of missingness across the genome due to its design, which thus leads to challenges for statistical analyses. Three topics are studied in this thesis: the development of formal statistical tools for detecting the existence of point mutation clusters under the microarray platform; the evaluation of the performance of test statistics developed while accounting for various probe designs, in terms of the capabilities of detecting mutation clusters; the development of formal statistical tools for testing the existence of spatial association between point mutations and mutations in segments. Statistical models such as Poisson point processes and Neyman-Scott processes are used for the distributions of the locations of point mutations under null and alternative hypotheses. Monte Carlo frameworks are established for statistical inference and the evaluation of power performance of the proposed test statistics. Tests with desirable performance are identified and recommended as screening tools. These statistical tools can be used for the study of other genomic events in the form of point events and events in segments, as well as with other microarray platforms than the MDGA which is utilized here. Simulated probe sets based on a window-based probe design mimicing the design of the MDGA are used to study the effect of various factors in probe design on the performance of test statistics. Insights are offered for determining key features in such design, such as probe intensity, when designing a new microarray platform, in order to achieve desired power for the purpose of mutation cluster detection.

Subjects/Keywords: Spatial statistics; Point processes; Cluster detection; Missing data; Mutation shower; Microarray probe design sampling; Hypothesis testing; Single nucleotide polymorphism; Copy number variation; Mouse Diversity Genotyping Array; Biostatistics

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Luo, B. (2018). Statistical tools for assessment of spatial properties of mutations observed under the microarray platform. (Thesis). University of Western Ontario. Retrieved from https://ir.lib.uwo.ca/etd/5746

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Luo, Bin. “Statistical tools for assessment of spatial properties of mutations observed under the microarray platform.” 2018. Thesis, University of Western Ontario. Accessed December 12, 2019. https://ir.lib.uwo.ca/etd/5746.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Luo, Bin. “Statistical tools for assessment of spatial properties of mutations observed under the microarray platform.” 2018. Web. 12 Dec 2019.

Vancouver:

Luo B. Statistical tools for assessment of spatial properties of mutations observed under the microarray platform. [Internet] [Thesis]. University of Western Ontario; 2018. [cited 2019 Dec 12]. Available from: https://ir.lib.uwo.ca/etd/5746.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Luo B. Statistical tools for assessment of spatial properties of mutations observed under the microarray platform. [Thesis]. University of Western Ontario; 2018. Available from: https://ir.lib.uwo.ca/etd/5746

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Vienna

17. Roblek, Marko. Generation and characterisation of laminopathy-specific antibodies.

Degree: 2009, University of Vienna

URL: http://othes.univie.ac.at/3818/

►

Laminopathien sind eine Gruppe von Krankheiten die hauptsächlich von Mutationen in den A-typ Laminen verursacht werden. Die meisten dieser Mutationen verursachen einen Austausch von nur… (more)

▼

Laminopathien sind eine Gruppe von Krankheiten die hauptsächlich von Mutationen in den A-typ Laminen verursacht werden. Die meisten dieser Mutationen verursachen einen Austausch von nur einer Aminosäure, die meistens autosomal-dominant vererbt werden. Die molekularen Mechanismen von Laminopathien und deren Krankheitsursache, sind aufgrund des Fehlens von spezifischen Forschungswerkzeugen, die das Studieren von den mutanten Proteinen in Patientenzellen erlauben würden, hingegen nur wenig bekannt. Basierend auf dem Ergebnis, dass die im Ogris Labor entwickelten Antikörper gegen Isoformen von Protein Phosphatase 2A (PP2A) Untereinheiten zwischen Isoformen unterscheiden können, die sich nur in einer Aminosäure des entsprechenden Epitops unterscheiden, meinten wir, dass es möglich wäre Antikörper zu entwickeln, die spezifisch das Wildtyp oder die mutanten A-typ Laminproteine, die sich nur in einer Aminosäure unterscheiden, erkennen würden. Solche Antikörper wären für die detailierte Charakterisierung des pathologischen Zustandes und zur Aufklärung der Krankheitsenstehung auf zellulärer Ebene sehr hilfreich. Deshalb hatten wir die Absicht, monoklonale Antikörper spezifisch für autosomal-dominant Emery Dreifuss Muskuläre Dystrophie (EDMD), familiäre partielle Lipodystrophie (FPLD), und dilatierte Kardiomyopathie (DCM) verursachende Punktmutationen in den A-typ Laminen zu entwickeln, die es erlauben würden, zwischen Proteinen, die nur in einer einzigen Aminosäure verschieden sind, zu unterscheiden, um so die speziellen molekularen Eigenschaften des Wildtyp oder des mutanten Proteins zu untersuchen. Um zu solchen Antikörpern zu gelangen wurden drei Ansätze der Antigenpräsentation angewandt. Diese inkludierten den Gebrauch von kurzen Peptiden gekoppelt an das Keyhole limpet hemocyanin (KLH) Protein, rekombinante Proteine bestehend aus der Lamin A/C C-terminalen Ig-fold Domäne fusioniert mit dem Hepatitis B Virus Core Protein (HBcAg), und rekombinante Proteine bestehend aus kurzen Peptiden, mit den gewünschten Lamin A/C Epitopen, fusioniert an HBcAg. Die Entwicklung eines EDMD relevanten punktmutation-spezifischen Antikörpers gegen die Lamin A/C Mutante R453W war mit dem KLH-Peptid Ansatz erfolgreich. Zusätzlich wurde ein Lamin A/C Ig-fold spezifischer monoklonaler Antikörper entwickelt. Bedeutend ist auch, dass Immunisierungen mit dem rekombinanten HBcAg-lamin A/C Ig-fold R482W Fusionsprotein zu einem punktmutations-spezifischen Antikörper führten. Das deutet darauch hin, dass Immunisierungen mit einer ganzen Proteindomäne eine punktmutation-spezifische Immunantwort auslösen können. Bedeutend ist, dass Experimente mit primären EDMD Patientenzellen zeigten, dass der neu entwickelte anti-Lamin A/C R453W Antikörper spezifisch die mutanten Proteine immunpräzipitieren, und die mutanten Proteine in der Immunfluoreszenz und im Immunblotting detektieren konnte. Mit dem anti-lamin A/C R453W Antikörper werden Funktionen der mutanten A-typ Lamine in primären Zellen von EDMD leidenden Patienten untersucht.

Laminopathies are a…

Subjects/Keywords: 42.13 Molekularbiologie; Punktmutations-spezifische Antikörper / Laminopathien; point-mutation specific antibodies / laminopathies

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Roblek, M. (2009). Generation and characterisation of laminopathy-specific antibodies. (Thesis). University of Vienna. Retrieved from http://othes.univie.ac.at/3818/

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Roblek, Marko. “Generation and characterisation of laminopathy-specific antibodies.” 2009. Thesis, University of Vienna. Accessed December 12, 2019. http://othes.univie.ac.at/3818/.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Roblek, Marko. “Generation and characterisation of laminopathy-specific antibodies.” 2009. Web. 12 Dec 2019.

Vancouver:

Roblek M. Generation and characterisation of laminopathy-specific antibodies. [Internet] [Thesis]. University of Vienna; 2009. [cited 2019 Dec 12]. Available from: http://othes.univie.ac.at/3818/.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Roblek M. Generation and characterisation of laminopathy-specific antibodies. [Thesis]. University of Vienna; 2009. Available from: http://othes.univie.ac.at/3818/

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

18. Πάλλιου, Ανδριάννα. Αναστολή της ενεργοποιημένης κινάσης της τυροσίνης JAK2 λόγω της σημειακής μετάλλαξης V617F στα χρόνια μυελοϋπερπλαστικά σύνδρομα.

Degree: 2011, National and Kapodistrian University of Athens; Εθνικό και Καποδιστριακό Πανεπιστήμιο Αθηνών (ΕΚΠΑ)

URL: http://hdl.handle.net/10442/hedi/27415

► Chronic Myeloproliferative Disorders CMPDs, which were first reported by Dameshek in 1951, are clonal hematopoietic stem cell disorders characterized by proliferation of 1 or more… (more)

▼ Chronic Myeloproliferative Disorders CMPDs, which were first reported by Dameshek in 1951, are clonal hematopoietic stem cell disorders characterized by proliferation of 1 or more myeloid cell lineages in the bone marrow and increased numbers of mature or immature cells in peripheral blood. According to the Word Health Organization, CMPDs or Myeloproliferative Neoplasms MPNs, include Polycythemia Vera (PV), Essential Thrombocythemia (ET), Idiopathic Myelofibrosis (IMF), plus rarer subtypes such us chronic neutrophilic leukaemia (CNL), hypereosinophilic syndrome (HES) and chronic eosinophilic leukaemia (CEL). The identification in 2005 of the V617F mutation in the JAK2 gene in Chronic Myeloproliferative Disorders (CMPDs) or Myeloproliferative Neoplasms (MPNs ) may explain several characteristics of these diseases and provide a prognostic marker in clinical practice. The JAK2V617F mutation is present in 90% of Polycythemia Vera, PV, patients and in about a half of Essential Thrombocythemia, ET, and Idiopathic Myelofibrosis, IMF, patients respectively. The first purpose of this study was the investigation of the V617F mutation of the JAK2 gene in our patients and the correlation between the clinical phenotype of patients with the JAK2V617F mutation and without. Also, is important to evaluate the mutation load of the V617F mutated alleles in all patients and to find the correlation between the mutation load and the clinical phenotype of the patients. Secondly, we evaluate the mutation load of the V617F mutated alleles in PV patients after a treatment with the pegylated forms of INFa-2a. The second purpose of this study was the investigation of the mutations of the exon 12 of the JAK2 gene in the negative cases for the V617 mutation in PV. The third purpose of this study was the investigation of the mutations in the thrombopoietin receptor-MPL in the negative cases for the V617 mutation in ET and IMF samples. Patients were found to carry the V617F mutation in the exon 14 of the JAK2 gene and the other patients were negative. The proportion of positive cases per disease sutype was PV 434/480 (90.5%), IMF 9/18 (50%) and ET 525/880 (60%). The negative patients for the V617F mutation was PV 46/480 (9.5%), ET 355/880 (40%) and IMF 9/18 (50%) were searched for the exon 12 mutation of the JAK2 gene (N542-E543del, K539L, F537-K539delInsL, H538QK539L) and for mutation in the thrombopoietin receptor (MPLW515L/W515K). These mutations were detected using the allele-specific real-time polymerase chain reaction (AS-PCR) assay described by (Scott et al, 2007). The conclusions of our study: • Investigation of the JAK2V617F mutation in patients with PV, IMF, and ET. Correlations between the clinical phenotype of patients with or without the mutation. We observed that the clinical phenotype of the PV patients carried the JAK2V617F mutation had a increased level of WBC, PLT count and Hct level, but not in the level of the Hb. In the IMF patients we did not observed any differences. In the ET patients we observed that the level of the Hb,…

Subjects/Keywords: Χρόνια μυελοϋπερπλαστικά σύνδρομα; JAK2 V617F σημειακή μετάλλαξη; Ιδιοπαθή μυελοσκλήρυνση; Ιδιοπαθή θρομβοκυτταραιμία; Ποσοτική ανάλυση του ποσοστού των μεταλλαγμένων αλληλομορφών JAK2 V617F φορτίου νόσου; Μετάλλαξη εξόνιο 12; Chronic myeloproliferatire odorders CMPD; JAK2 V617F point mutation; Idiopathick myelofibrosis; Idiopathic thromboeythemia; Image quant JAK2 V617F; Allele burden; Mutation exon 12

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Πάλλιου, . �. (2011). Αναστολή της ενεργοποιημένης κινάσης της τυροσίνης JAK2 λόγω της σημειακής μετάλλαξης V617F στα χρόνια μυελοϋπερπλαστικά σύνδρομα. (Thesis). National and Kapodistrian University of Athens; Εθνικό και Καποδιστριακό Πανεπιστήμιο Αθηνών (ΕΚΠΑ). Retrieved from http://hdl.handle.net/10442/hedi/27415

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Πάλλιου, Ανδριάννα. “Αναστολή της ενεργοποιημένης κινάσης της τυροσίνης JAK2 λόγω της σημειακής μετάλλαξης V617F στα χρόνια μυελοϋπερπλαστικά σύνδρομα.” 2011. Thesis, National and Kapodistrian University of Athens; Εθνικό και Καποδιστριακό Πανεπιστήμιο Αθηνών (ΕΚΠΑ). Accessed December 12, 2019. http://hdl.handle.net/10442/hedi/27415.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Πάλλιου, Ανδριάννα. “Αναστολή της ενεργοποιημένης κινάσης της τυροσίνης JAK2 λόγω της σημειακής μετάλλαξης V617F στα χρόνια μυελοϋπερπλαστικά σύνδρομα.” 2011. Web. 12 Dec 2019.

Vancouver:

Πάλλιου �. Αναστολή της ενεργοποιημένης κινάσης της τυροσίνης JAK2 λόγω της σημειακής μετάλλαξης V617F στα χρόνια μυελοϋπερπλαστικά σύνδρομα. [Internet] [Thesis]. National and Kapodistrian University of Athens; Εθνικό και Καποδιστριακό Πανεπιστήμιο Αθηνών (ΕΚΠΑ); 2011. [cited 2019 Dec 12]. Available from: http://hdl.handle.net/10442/hedi/27415.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Πάλλιου �. Αναστολή της ενεργοποιημένης κινάσης της τυροσίνης JAK2 λόγω της σημειακής μετάλλαξης V617F στα χρόνια μυελοϋπερπλαστικά σύνδρομα. [Thesis]. National and Kapodistrian University of Athens; Εθνικό και Καποδιστριακό Πανεπιστήμιο Αθηνών (ΕΚΠΑ); 2011. Available from: http://hdl.handle.net/10442/hedi/27415

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

19. 酒井, 純. A novel detection procedure for mutations in the 23S rRNA gene of Mycoplasma pneumoniae with peptide nucleic acid-mediated loop-mediated isothermal amplification assay. : ペプチド核酸peptide nucleic acid (PNA)とloop-mediated isothermal amplification assay（LAMP）法を組み合わせた、Mycoplasma pneumoniaeの23S rRNA変異の革新的検出法.

Degree: 博士(医学), 2018, Saitama Medical University / 埼玉医科大学

URL: http://id.nii.ac.jp/1386/00000620/

►

Rapid and easy detection of a single nucleotide point mutation of bacterial genes, which is directly linked to drug susceptibility, is essential for the proper… (more)

Subjects/Keywords: Colorimetry; DNA, Bacterial; Genes, Bacterial; Genes, rRNA; Genotype; Humans; Limit of Detection; Mycoplasma pneumoniae; Nasopharynx; Nucleic Acid Amplification Techniques; Peptide Nucleic Acids; Pneumonia, Mycoplasma; Point Mutation; Point-of-Care Testing; Polymerase Chain Reaction; RNA, Ribosomal, 23S; Sensitivity and Specificity; Temperature

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

酒井, �. (2018). A novel detection procedure for mutations in the 23S rRNA gene of Mycoplasma pneumoniae with peptide nucleic acid-mediated loop-mediated isothermal amplification assay. : ペプチド核酸peptide nucleic acid (PNA)とloop-mediated isothermal amplification assay（LAMP）法を組み合わせた、Mycoplasma pneumoniaeの23S rRNA変異の革新的検出法. (Thesis). Saitama Medical University / 埼玉医科大学. Retrieved from http://id.nii.ac.jp/1386/00000620/

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

酒井, 純. “A novel detection procedure for mutations in the 23S rRNA gene of Mycoplasma pneumoniae with peptide nucleic acid-mediated loop-mediated isothermal amplification assay. : ペプチド核酸peptide nucleic acid (PNA)とloop-mediated isothermal amplification assay（LAMP）法を組み合わせた、Mycoplasma pneumoniaeの23S rRNA変異の革新的検出法.” 2018. Thesis, Saitama Medical University / 埼玉医科大学. Accessed December 12, 2019. http://id.nii.ac.jp/1386/00000620/.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

酒井, 純. “A novel detection procedure for mutations in the 23S rRNA gene of Mycoplasma pneumoniae with peptide nucleic acid-mediated loop-mediated isothermal amplification assay. : ペプチド核酸peptide nucleic acid (PNA)とloop-mediated isothermal amplification assay（LAMP）法を組み合わせた、Mycoplasma pneumoniaeの23S rRNA変異の革新的検出法.” 2018. Web. 12 Dec 2019.

Vancouver:

酒井 �. A novel detection procedure for mutations in the 23S rRNA gene of Mycoplasma pneumoniae with peptide nucleic acid-mediated loop-mediated isothermal amplification assay. : ペプチド核酸peptide nucleic acid (PNA)とloop-mediated isothermal amplification assay（LAMP）法を組み合わせた、Mycoplasma pneumoniaeの23S rRNA変異の革新的検出法. [Internet] [Thesis]. Saitama Medical University / 埼玉医科大学; 2018. [cited 2019 Dec 12]. Available from: http://id.nii.ac.jp/1386/00000620/.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

酒井 �. A novel detection procedure for mutations in the 23S rRNA gene of Mycoplasma pneumoniae with peptide nucleic acid-mediated loop-mediated isothermal amplification assay. : ペプチド核酸peptide nucleic acid (PNA)とloop-mediated isothermal amplification assay（LAMP）法を組み合わせた、Mycoplasma pneumoniaeの23S rRNA変異の革新的検出法. [Thesis]. Saitama Medical University / 埼玉医科大学; 2018. Available from: http://id.nii.ac.jp/1386/00000620/

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Gothenburg / Göteborgs Universitet

20. Kollberg, Gittan. Crisis in Energy Metabolism - Mitochondrial Defects and a New Disease Entity.

Degree: 2006, University of Gothenburg / Göteborgs Universitet

URL: http://hdl.handle.net/2077/779

► Impairment of energy metabolism may be associated with severe implications for affected individuals since all fundamental cell functions are energy-dependent. Disorders of energy metabolism are… (more)

▼ Impairment of energy metabolism may be associated with severe implications for affected individuals since all fundamental cell functions are energy-dependent. Disorders of energy metabolism are often genetic and associated with defects in the oxidative phosphorylation in mitochondria. This thesis addresses the pathogenesis in some mitochondrial disorders and a new disease entity associated with defects in the glycogen metabolism. In paper I we report on a primary mutation in mitochondrial DNA. We identified a T→C mutation at position 582 in the gene for tRNAPhe in a case of mitochondrial myopathy. The mutation alters a conserved base pairing in the aminoacyl stem of the tRNA. By analysis of single muscle fibers we showed that the level of heteroplasmy (proportion of mutant mtDNA) was higher in muscle fibers with defective cytochrome c oxidase (COX) activity compared to normal muscle fibers. Based on these findings we conclude that this mutation was responsible for the disease. In paper II we investigated a 30-year-old woman, who presented with an attack of acute rhabdomyolysis. We found an isolated deficiency of COX and a novel nonsense mutation in mtDNA in the gene encoding COX subunit I. In addition to its catalytic function, our data clearly indicates an important function of subunit I for the assembly of COX. The mutation was restricted to the patient’s muscle, but was not detectable in myoblasts, cultured from satellite cells isolated from affected muscle tissue. This result may have interesting implications for the natural evolution of the disease and perhaps therapy, since regenerating muscle occurs by proliferation of satellite cells. In paper III we investigated patients with mitochondrial diseases (progressive external ophthalmoplegia, PEO) due to primary mutations in POLG1 encoding mtDNA polymerase gamma (Polγ) and secondary multiple mtDNA deletions. The results show that it is very unlikely that mtDNA point mutations contribute to the pathogenesis in PEO patients with primary POLG1 mutations, and that the mechanism by which mutant Polγ cause mtDNA deletions does not involve mtDNA point mutations as an intermediate step, as has been previously proposed. In paper IV mtDNA alterations and pathology of muscle, brain and liver was investigated in children with Alpers-Huttenlocher syndrome (AHS), a fatal neurodegenerative disease associated with liver failure. All children had compound heterozygous missense mutations in POLG1. We provide evidence that AHS is a mitochondrial disease by demonstrating mtDNA alterations (reduced mtDNA copy number and multiple mtDNA deletions). Liver disease was triggered by valproate treatment in several cases possibly due to severe respiratory chain deficiency, which was demonstrated in liver tissue in one case. In paper V we report on a new disease entity “Muscle glycogen storage disease type zero” due to a homozygous stop mutation in the muscle glycogen synthase gene (GYS1). We performed investigations on a family where one child suffered sudden cardiac death at the age of…

Subjects/Keywords: Energy metabolism; Mitochondrial Disorders; mtDNA; multiple mtDNA deletions; Alpers-Huttenlocher syndrome; POLG1; GYS1; glycogen synthase; point mutation; Polymerase gamma

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Kollberg, G. (2006). Crisis in Energy Metabolism - Mitochondrial Defects and a New Disease Entity. (Thesis). University of Gothenburg / Göteborgs Universitet. Retrieved from http://hdl.handle.net/2077/779

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Kollberg, Gittan. “Crisis in Energy Metabolism - Mitochondrial Defects and a New Disease Entity.” 2006. Thesis, University of Gothenburg / Göteborgs Universitet. Accessed December 12, 2019. http://hdl.handle.net/2077/779.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Kollberg, Gittan. “Crisis in Energy Metabolism - Mitochondrial Defects and a New Disease Entity.” 2006. Web. 12 Dec 2019.

Vancouver:

Kollberg G. Crisis in Energy Metabolism - Mitochondrial Defects and a New Disease Entity. [Internet] [Thesis]. University of Gothenburg / Göteborgs Universitet; 2006. [cited 2019 Dec 12]. Available from: http://hdl.handle.net/2077/779.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Kollberg G. Crisis in Energy Metabolism - Mitochondrial Defects and a New Disease Entity. [Thesis]. University of Gothenburg / Göteborgs Universitet; 2006. Available from: http://hdl.handle.net/2077/779

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

21. Ahronian, Leanne G. Identification and Characteristics of Factors Regulating Hepatocellular Carcinoma Progression and Metastasis: A Dissertation.

Degree: Cancer Biology, Molecular, Cell and Cancer Biology Department, 2014, U of Massachusetts : Med

URL: http://escholarship.umassmed.edu/gsbs_diss/705

► Hepatocellular carcinoma (HCC) is a common malignancy of the liver that is one of the most frequent causes of cancer-related death in the world.… (more)

▼ Hepatocellular carcinoma (HCC) is a common malignancy of the liver that is one of the most frequent causes of cancer-related death in the world. Surgical resection and liver transplantation are the only curative options for HCC, and tumor invasion and metastasis render many patients ineligible for these treatments. Identification of the mechanisms that contribute to invasive and metastatic disease may enlighten therapeutic strategies for those not eligible for surgical treatments. In this dissertation, I describe two sets of experiments to elucidate mechanisms underlying HCC dissemination, involving the activities of Krüppel-like factor 6 and a particular p53 point mutation, R172H. Gene expression profiling of migratory HCC subpopulations demonstrated reduced expression of Krüppel-like factor 6 (KLF6) in invasive HCC cells. Knockdown of KLF6 in HCC cells increased cell transformation and migration. Single-copy deletion of Klf6 in a HCC mouse model results in increased tumor formation, increased metastasis to the lungs, and decreased survival, indicating that KLF6 suppresses both tumor formation and metastasis in HCC. To elucidate the mechanism of KLF6-mediated tumor and metastasis suppression, we performed gene expression profiling and ChIP-sequencing to identify direct transcriptional targets of KLF6 in HCC cells. This analysis revealed novel transcriptional targets of KLF6 in HCC including CDC42EP3 and VAV3, both of which are positive regulators of Rho family GTPases. Concordantly, KLF6 knockdown cells demonstrate increased activity of the Rho family GTPases RAC1 and CDC42, and RAC1 is required for migration induced following KLF6 knockdown. Moreover, VAV3 and CDC42EP3 are also required for enhanced cell migration in HCC cells with KLF6 knockdown. Together, this work describes a novel signaling axis through which KLF6-mediated repression of VAV3 and CDC42EP3 inhibits RAC1Gmediated HCC cell migration in culture, and potentially HCC metastasis in vivo. TP53 gene mutations are commonly found in HCC and are associated with poor prognosis. Prior studies have suggested that p53 mutants can display gain-of- function properties in other tumor types. Therefore, I sought to determine if a particular hotspot p53 mutation, p53^R172H, provided enhanced, gain-of-function properties compared to p53 loss in HCC. In vitro, soft agar colony formation and cell migration is reduced upon knockdown of p53^R172H, indicating that this mutation is required for transformation-associated phenotypes in these cells. However, p53^R172H-expressing mice did not have enhanced tumor formation or metastasis compared to p53-null mice. These data suggest that p53^R172H and p53 deletion are functionally equivalent in vivo, and that p53^R172H is not a gain-of-function mutant in HCC. Inhibition of the related transcription factors p63 and p73 has been suggested as a potential mechanism by which mutant p53… Advisors/Committee Members: Brian C. Lewis, PhD.

Subjects/Keywords: Hepatocellular Carcinoma; Gene Expression Profiling; p53 Genes; Kruppel-Like Transcription Factors; Liver Neoplasms; Point Mutation; rho GTP-Binding Proteins; Transcription Factors; Tumor Suppressor Protein p53; Cancer Biology; Digestive System Diseases; Molecular Genetics; Neoplasms

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Ahronian, L. G. (2014). Identification and Characteristics of Factors Regulating Hepatocellular Carcinoma Progression and Metastasis: A Dissertation. (Doctoral Dissertation). U of Massachusetts : Med. Retrieved from http://escholarship.umassmed.edu/gsbs_diss/705

Chicago Manual of Style (16^th Edition):

Ahronian, Leanne G. “Identification and Characteristics of Factors Regulating Hepatocellular Carcinoma Progression and Metastasis: A Dissertation.” 2014. Doctoral Dissertation, U of Massachusetts : Med. Accessed December 12, 2019. http://escholarship.umassmed.edu/gsbs_diss/705.

MLA Handbook (7^th Edition):

Ahronian, Leanne G. “Identification and Characteristics of Factors Regulating Hepatocellular Carcinoma Progression and Metastasis: A Dissertation.” 2014. Web. 12 Dec 2019.

Vancouver:

Ahronian LG. Identification and Characteristics of Factors Regulating Hepatocellular Carcinoma Progression and Metastasis: A Dissertation. [Internet] [Doctoral dissertation]. U of Massachusetts : Med; 2014. [cited 2019 Dec 12]. Available from: http://escholarship.umassmed.edu/gsbs_diss/705.

Council of Science Editors:

Ahronian LG. Identification and Characteristics of Factors Regulating Hepatocellular Carcinoma Progression and Metastasis: A Dissertation. [Doctoral Dissertation]. U of Massachusetts : Med; 2014. Available from: http://escholarship.umassmed.edu/gsbs_diss/705

University of Oxford

22. Rendel, Mark D. The evolutionary dynamics of neutral networks : lessons from RNA.

Degree: PhD, 2008, University of Oxford

URL: http://ora.ox.ac.uk/objects/uuid:85107ca7-fada-4582-95e7-17b5bbb038cd ; http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.580854

► The evolutionary options of a population are strongly influenced by the avail- ability of adaptive mutants. In this thesis, I use the concept of neutral… (more)

▼ The evolutionary options of a population are strongly influenced by the avail- ability of adaptive mutants. In this thesis, I use the concept of neutral networks to show that neutral drift can actually increase the accessibility of adaptive mu- tants, and therefore facilitate adaptive evolutionary change. Neutral networks are groups of unique genotypes which all code for the same phenotype, and are connected by simple point mutations. I calculate the size and shape of the networks in a small but exhaustively enumerated space of RNA genotypes by mapping the sequences to RNA secondary structure phenotypes. The qual- itative results are similar to those seen in many other genotype–phenotype map models, despite some significant methodological differences. I show that the boundary of each network has single point–mutation connections to many more phenotypes than the average individual genotype within that network. This means that paths involving a series of neutral point–mutation steps across a network can allow evolution to adaptive phenotypes which would otherwise be extremely unlikely to arise spontaneously. This can be likened to walking along a flat ridge in an adaptive landscape, rather than traversing or jumping across a lower fitness valley. Within this model, when a genotype is made up of just 10 bases, the mean neutral path length is 1.88 point mutations. Furthermore, the map includes some networks that are so convoluted that the path through the network is longer than the direct route between two sequences. A minimum length adaptive walk across the genotype space usually takes as many neutral steps as adaptive ones on its way to the optimum phenotype. Finally I show that the shape of a network can have a very important affect on the number of generations it takes a population to drift across it, and that the more routes between two sequences, the fewer generations required for a population to find an advantageous sequence. My conclusion is that, within the RNA map at least, the size, shape and connectivity of neutral networks all have a profound effect on the way that sequences change and populations evolve, and by not considering them, we risk missing an important evolutionary mechanism.

Subjects/Keywords: 570.285; Bioinformatics (life sciences); Biology; Genetics (life sciences); Evolution (zoology); Biology and other natural sciences (mathematics); Mathematical biology; Mathematical genetics and bioinformatics (statistics); Applications and algorithms; RNA folding; adaptive; landscape; fitness; simulation; epistasis; genotype; phenotype; map; point mutation

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Rendel, M. D. (2008). The evolutionary dynamics of neutral networks : lessons from RNA. (Doctoral Dissertation). University of Oxford. Retrieved from http://ora.ox.ac.uk/objects/uuid:85107ca7-fada-4582-95e7-17b5bbb038cd ; http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.580854

Chicago Manual of Style (16^th Edition):

Rendel, Mark D. “The evolutionary dynamics of neutral networks : lessons from RNA.” 2008. Doctoral Dissertation, University of Oxford. Accessed December 12, 2019. http://ora.ox.ac.uk/objects/uuid:85107ca7-fada-4582-95e7-17b5bbb038cd ; http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.580854.

MLA Handbook (7^th Edition):

Rendel, Mark D. “The evolutionary dynamics of neutral networks : lessons from RNA.” 2008. Web. 12 Dec 2019.

Vancouver:

Rendel MD. The evolutionary dynamics of neutral networks : lessons from RNA. [Internet] [Doctoral dissertation]. University of Oxford; 2008. [cited 2019 Dec 12]. Available from: http://ora.ox.ac.uk/objects/uuid:85107ca7-fada-4582-95e7-17b5bbb038cd ; http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.580854.

Council of Science Editors:

Rendel MD. The evolutionary dynamics of neutral networks : lessons from RNA. [Doctoral Dissertation]. University of Oxford; 2008. Available from: http://ora.ox.ac.uk/objects/uuid:85107ca7-fada-4582-95e7-17b5bbb038cd ; http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.580854

University of Cambridge

23. Buell, Alexander Kai. On the kinetics of protein misfolding and aggregation.

Degree: PhD, 2011, University of Cambridge

URL: https://www.repository.cam.ac.uk/handle/1810/270324 ; http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.609706

► Protein (mis)folding into highly ordered, fibrillar structures, amyloid fibrils, is a hallmark of several, mainly neurodegenerative, disorders. The mechanism of this supra-molecular self-assembly reaction, as… (more)

▼ Protein (mis)folding into highly ordered, fibrillar structures, amyloid fibrils, is a hallmark of several, mainly neurodegenerative, disorders. The mechanism of this supra-molecular self-assembly reaction, as well as its relationship to protein folding are not well understood. In particular, the molecular origin of the metastability of the soluble state of proteins with respect to the aggregated states has not been clearly established. In this dissertation, it is demonstrated, that highly accurate kinetic experiments, using a novel biosensing method, can yield fundamental insight into the dynamics of proteins in the region of the free energy landscape corresponding to protein aggregation. First, a section on Method development describes the extension and elaboration of the previously established kinetic assay relying on quartz crystal microbalance measurements for the study of amyloid fibril elongation (Chapter 3). This methodology is then applied in order to study in great detail the origin of the various contributions to the free energy barriers separating the soluble state of a protein from its aggregated state. In particular, the relative importance of residual structure, hydrophobicity (Chapter 4) and electrostatic interactions (Chapter 5) for the total free energy of activation are discussed. In the last part of this thesis (Chapter 6), it is demonstrated that this biosensing method can also be used to study the binding of small molecules to amyloid fibrils, a very useful feature in the framework of the quest for potential inhibitors of amyloid formation. In addition, it is shown that Thioflavin T, to-date the most frequently employed fluorescent label molecule for bulk solution kinetic studies, can in the presence of potential amyloid inhibitor candidates be highly unreliable as a means to quantify the effect of the inhibitor on amyloid formation kinetics. In summary, the work in this thesis contributes to both the fundamental and the applied aspects of the field of protein aggregation.

Subjects/Keywords: 620; amyloid; kinetics; protein; aggregation; quartz crystal microbalance; energy barrier; folding; misfolding; Alzheimer disease; Parkinson disease; peptide; entropy; enthalpy; activation energy; point mutation; biosensing; inhibitor; Thioflavin-T; Congo Red; Debye-Hu¨ckel; ionic strength; calorimetry; zeta-potential; light scattering

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Buell, A. K. (2011). On the kinetics of protein misfolding and aggregation. (Doctoral Dissertation). University of Cambridge. Retrieved from https://www.repository.cam.ac.uk/handle/1810/270324 ; http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.609706

Chicago Manual of Style (16^th Edition):

Buell, Alexander Kai. “On the kinetics of protein misfolding and aggregation.” 2011. Doctoral Dissertation, University of Cambridge. Accessed December 12, 2019. https://www.repository.cam.ac.uk/handle/1810/270324 ; http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.609706.

MLA Handbook (7^th Edition):

Buell, Alexander Kai. “On the kinetics of protein misfolding and aggregation.” 2011. Web. 12 Dec 2019.

Vancouver:

Buell AK. On the kinetics of protein misfolding and aggregation. [Internet] [Doctoral dissertation]. University of Cambridge; 2011. [cited 2019 Dec 12]. Available from: https://www.repository.cam.ac.uk/handle/1810/270324 ; http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.609706.

Council of Science Editors:

Buell AK. On the kinetics of protein misfolding and aggregation. [Doctoral Dissertation]. University of Cambridge; 2011. Available from: https://www.repository.cam.ac.uk/handle/1810/270324 ; http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.609706

24. Zhou, Yi. A Study of Comparative Analysis of Transposable Elements in filamentous fungi.

Degree: Genetics, Genomics and Bioinformatics, 2012, University of California – Riverside

URL: http://www.escholarship.org/uc/item/3gn6734m

► Transposable elements (TE) are genetic elements, which can move within the genome. TE are widely distributed in almost all organisms, both prokaryotes and eukrayotes Nearly… (more)

Subjects/Keywords: Bioinformatics; filamentous fungi; LTR-containing retroelements; repeat-induced point mutation; TE annotation pipeline; Transposable elements

…transposable elements in fungi �� .…8 Repeat-Induce Point mutation (RIP)… …Conclusion �� …... �� …...42 Chapter V Repeat-Induced Point Mutation (RIP… …x28;Gogvadze et al. 2009) and Repeat-induced point mutation (RIP). The… …induced Point Mutation (RIP) mechanism. Different organisms have developed their own… …Repeat9 induced point mutation (RIP), quelling and methylation (Muszewska et al…

4 more images…

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Zhou, Y. (2012). A Study of Comparative Analysis of Transposable Elements in filamentous fungi. (Thesis). University of California – Riverside. Retrieved from http://www.escholarship.org/uc/item/3gn6734m

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Zhou, Yi. “A Study of Comparative Analysis of Transposable Elements in filamentous fungi.” 2012. Thesis, University of California – Riverside. Accessed December 12, 2019. http://www.escholarship.org/uc/item/3gn6734m.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Zhou, Yi. “A Study of Comparative Analysis of Transposable Elements in filamentous fungi.” 2012. Web. 12 Dec 2019.

Vancouver:

Zhou Y. A Study of Comparative Analysis of Transposable Elements in filamentous fungi. [Internet] [Thesis]. University of California – Riverside; 2012. [cited 2019 Dec 12]. Available from: http://www.escholarship.org/uc/item/3gn6734m.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Zhou Y. A Study of Comparative Analysis of Transposable Elements in filamentous fungi. [Thesis]. University of California – Riverside; 2012. Available from: http://www.escholarship.org/uc/item/3gn6734m

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Cambridge

25. Buell, Alexander Kai. On the Kinetics of Protein Misfolding and Aggregation .

Degree: 2011, University of Cambridge

URL: https://www.repository.cam.ac.uk/handle/1810/270324

► Protein (mis)folding into highly ordered, fibrillar structures, amyloid fibrils, is a hallmark of several, mainly neurodegenerative, disorders. The mechanism of this supra-molecular self-assembly reaction, as… (more)

▼ Protein (mis)folding into highly ordered, fibrillar structures, amyloid fibrils, is a hallmark of several, mainly neurodegenerative, disorders. The mechanism of this supra-molecular self-assembly reaction, as well as its relationship to protein folding are not well understood. In particular, the molecular origin of the metastability of the soluble state of proteins with respect to the aggregated states has not been clearly established. In this dissertation, it is demonstrated, that highly accurate kinetic experiments, using a novel biosensing method, can yield fundamental insight into the dynamics of proteins in the region of the free energy landscape corresponding to protein aggregation. First, a section on Method development describes the extension and elaboration of the previously established kinetic assay relying on quartz crystal microbalance measurements for the study of amyloid fibril elongation (Chapter 3). This methodology is then applied in order to study in great detail the origin of the various contributions to the free energy barriers separating the soluble state of a protein from its aggregated state. In particular, the relative importance of residual structure, hydrophobicity (Chapter 4) and electrostatic interactions (Chapter 5) for the total free energy of activation are discussed. In the last part of this thesis (Chapter 6), it is demonstrated that this biosensing method can also be used to study the binding of small molecules to amyloid fibrils, a very useful feature in the framework of the quest for potential inhibitors of amyloid formation. In addition, it is shown that Thioflavin T, to-date the most frequently employed fluorescent label molecule for bulk solution kinetic studies, can in the presence of potential amyloid inhibitor candidates be highly unreliable as a means to quantify the effect of the inhibitor on amyloid formation kinetics. In summary, the work in this thesis contributes to both the fundamental and the applied aspects of the field of protein aggregation.

Subjects/Keywords: amyloid; kinetics; protein; aggregation; quartz crystal microbalance; energy barrier; folding; misfolding; Alzheimer disease; Parkinson disease; peptide; entropy; enthalpy; activation energy; point mutation; biosensing; inhibitor; Thioflavin-T; Congo Red; Debye-Hückel; ionic strength; calorimetry; zeta-potential; light scattering

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Buell, A. K. (2011). On the Kinetics of Protein Misfolding and Aggregation . (Thesis). University of Cambridge. Retrieved from https://www.repository.cam.ac.uk/handle/1810/270324

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Buell, Alexander Kai. “On the Kinetics of Protein Misfolding and Aggregation .” 2011. Thesis, University of Cambridge. Accessed December 12, 2019. https://www.repository.cam.ac.uk/handle/1810/270324.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Buell, Alexander Kai. “On the Kinetics of Protein Misfolding and Aggregation .” 2011. Web. 12 Dec 2019.

Vancouver:

Buell AK. On the Kinetics of Protein Misfolding and Aggregation . [Internet] [Thesis]. University of Cambridge; 2011. [cited 2019 Dec 12]. Available from: https://www.repository.cam.ac.uk/handle/1810/270324.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Buell AK. On the Kinetics of Protein Misfolding and Aggregation . [Thesis]. University of Cambridge; 2011. Available from: https://www.repository.cam.ac.uk/handle/1810/270324

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Cape Town

26. Wells, Carol Dawn. The functional significance of the G to A point mutation in the promoter region of the Apolipoprotein AI gene.

Degree: Image, Division of Medical Biochemistry & Structural Biology, 1993, University of Cape Town

URL: http://hdl.handle.net/11427/27143

► AG to A transition at position -76 in the promoter region of the apoAI gene was previously identified, and the A-76 has been shown to… (more)

Subjects/Keywords: Apolipoproteins - genetics; DNA-Binding Proteins - analysis; Genetic engineering; Point Mutation; Promoter Regions (Genetics); Protein engineering

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Wells, C. D. (1993). The functional significance of the G to A point mutation in the promoter region of the Apolipoprotein AI gene. (Thesis). University of Cape Town. Retrieved from http://hdl.handle.net/11427/27143

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Wells, Carol Dawn. “The functional significance of the G to A point mutation in the promoter region of the Apolipoprotein AI gene.” 1993. Thesis, University of Cape Town. Accessed December 12, 2019. http://hdl.handle.net/11427/27143.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Wells, Carol Dawn. “The functional significance of the G to A point mutation in the promoter region of the Apolipoprotein AI gene.” 1993. Web. 12 Dec 2019.

Vancouver:

Wells CD. The functional significance of the G to A point mutation in the promoter region of the Apolipoprotein AI gene. [Internet] [Thesis]. University of Cape Town; 1993. [cited 2019 Dec 12]. Available from: http://hdl.handle.net/11427/27143.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Wells CD. The functional significance of the G to A point mutation in the promoter region of the Apolipoprotein AI gene. [Thesis]. University of Cape Town; 1993. Available from: http://hdl.handle.net/11427/27143

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

The Ohio State University

27. Beres, Zachery T. Ecological and Evolutionary Implications of Glyphosate Resistance in Conyza canadensis and Arabidopsis thaliana.

Degree: PhD, Evolution, Ecology and Organismal Biology, 2019, The Ohio State University

URL: http://rave.ohiolink.edu/etdc/view?acc_num=osu1555600547328876

► Herbicide resistant weeds are among the greatest threats facing modern agriculture. Glyphosate, the active ingredient in the commercial herbicide RoundUp, is the most widely… (more)

▼ Herbicide resistant weeds are among the greatest threats facing modern agriculture. Glyphosate, the active ingredient in the commercial herbicide RoundUp, is the most widely applied herbicide worldwide, and 43 weed species have evolved resistance to glyphosate to date. My research addressed four key questions about the strength, mechanisms, and fitness effects of glyphosate resistance using Conyza canadensis, a representative glyphosate-resistant weed, and Arabidopsis thaliana, a model plant species. In northcentral Ohio and southern Iowa, maternal biotypes (individual plants) of Conyza canadensis, also known as horseweed or marestail, were collected from 74 locations (both agricultural and non-agricultural) in both states. Dose-response experiments were used to categorize biotypes into resistance categories based on 80% survival at 0x only (Susceptible, S), and up to 1x (equivalent to 840 g ae ha^-1; R1), 8x (R2), 20x (R3), and 40x (R4). Extreme glyphosate resistance (R4 biotypes) was quite common in both states and both habitats, and non-agricultural habitats served as a refuge for R4 biotypes. Glyphosate resistance mechanisms are generally presumed to impose a fitness cost due to possible trade-offs in resource allocation to plant defense, growth, and reproduction. Nine S, eight R1, and nine R4 biotypes originally collected in Iowa were grown in a common garden experiment in Iowa over two years and two sites to test for fitness effects. Based on early rosette sizes and days to bolting, nested ANOVAs showed that R4 biotypes grew as large as, if not larger than, both S and R1 biotypes, and bolted significantly earlier. Furthermore, R1 and R4 biotypes were less likely to display disease symptoms than S biotypes. Glyphosate resistance in horseweed appears not to impose an early growth penalty, and possibly no lifetime fitness cost. Specific mechanisms of glyphosate resistance in horseweed include altered translocation and vacuolar sequestration. Recently, a Canadian study documented the first report of a target-site point mutation of a proline to serine substitution at position 106 of the EPSPS2 gene in horseweed. 24 Ohio biotypes and 20 Iowa biotypes were screened for this Pro-106-Ser mutation. All 19 biotypes scored as S, R1, or R2 lacked the Pro-106-Ser mutation, and all 25 biotypes scored as R3 or R4 possessed the Pro-106-Ser mutation. The association between the point mutation and extremely resistant horseweed biotypes is noteworthy. Model species, such as Arabidopsis thaliana, may be useful for understanding the fitness effects of other glyphosate resistance mechanisms such as the over-production of 5-enolpyrvulshikimate-3-phospahte synthase (EPSPS), which has been documented in 13 weed species. Transgenic Arabidopsis lines that overexpress (OX) a native EPSPS gene were created, as were empty vector lines (EV). Six OX lines and seven EV lines were grown in greenhouse fitness experiments in the absence of glyphosate. Two of the OX… Advisors/Committee Members: Snow, Allison (Advisor).

Subjects/Keywords: Agriculture; Agronomy; Biology; Ecology; Evolution and Development; herbicide; glyphosate; resistance; herbicide resistance; weeds; Conyza canadensis; Arabidopsis thaliana; fitness; resistance mechanisms; EPSPS; Pro-106-Ser; point mutation; 5-enolpyruvylshikimate-3-phosphate synthase

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Beres, Z. T. (2019). Ecological and Evolutionary Implications of Glyphosate Resistance in Conyza canadensis and Arabidopsis thaliana. (Doctoral Dissertation). The Ohio State University. Retrieved from http://rave.ohiolink.edu/etdc/view?acc_num=osu1555600547328876

Chicago Manual of Style (16^th Edition):

Beres, Zachery T. “Ecological and Evolutionary Implications of Glyphosate Resistance in Conyza canadensis and Arabidopsis thaliana.” 2019. Doctoral Dissertation, The Ohio State University. Accessed December 12, 2019. http://rave.ohiolink.edu/etdc/view?acc_num=osu1555600547328876.

MLA Handbook (7^th Edition):

Beres, Zachery T. “Ecological and Evolutionary Implications of Glyphosate Resistance in Conyza canadensis and Arabidopsis thaliana.” 2019. Web. 12 Dec 2019.

Vancouver:

Beres ZT. Ecological and Evolutionary Implications of Glyphosate Resistance in Conyza canadensis and Arabidopsis thaliana. [Internet] [Doctoral dissertation]. The Ohio State University; 2019. [cited 2019 Dec 12]. Available from: http://rave.ohiolink.edu/etdc/view?acc_num=osu1555600547328876.

Council of Science Editors:

Beres ZT. Ecological and Evolutionary Implications of Glyphosate Resistance in Conyza canadensis and Arabidopsis thaliana. [Doctoral Dissertation]. The Ohio State University; 2019. Available from: http://rave.ohiolink.edu/etdc/view?acc_num=osu1555600547328876

28. Fereidouni, Mohammad, 1977-. Evaluation of the coat protein of the Tombusviridae as HR elicitor in Nicotiana section.

Degree: 2014, University of Missouri – Columbia

URL: http://hdl.handle.net/10355/44266

► Plants are able to recognize and respond to virus infection with a hypersensitive response, a plant defense response that triggers a cell death pathway and… (more)

Subjects/Keywords: Author supplied: Tombusriridae, hypersensitive response, tobacco necrosis virus, coat protein, point mutation, modeller; Tombusviridae; Sensitive plant; Tobacco

…point mutation in the tobacco mosaic virus capsid protein gene induces hypersensitivity in… …structure of the host protein. At this point the R protein, also known as the guard protein… …negative. Furthermore, mutation of the ATG4 start codon to TTG also abolished HR, indicating that… …confirmed by point mutagenesis. ! 24 2- RESULTS 2-1. A sequence alignment of the TBSV and TNV…

15 more images…

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Fereidouni, Mohammad, 1. (2014). Evaluation of the coat protein of the Tombusviridae as HR elicitor in Nicotiana section. (Thesis). University of Missouri – Columbia. Retrieved from http://hdl.handle.net/10355/44266

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Fereidouni, Mohammad, 1977-. “Evaluation of the coat protein of the Tombusviridae as HR elicitor in Nicotiana section.” 2014. Thesis, University of Missouri – Columbia. Accessed December 12, 2019. http://hdl.handle.net/10355/44266.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Fereidouni, Mohammad, 1977-. “Evaluation of the coat protein of the Tombusviridae as HR elicitor in Nicotiana section.” 2014. Web. 12 Dec 2019.

Vancouver:

Fereidouni, Mohammad 1. Evaluation of the coat protein of the Tombusviridae as HR elicitor in Nicotiana section. [Internet] [Thesis]. University of Missouri – Columbia; 2014. [cited 2019 Dec 12]. Available from: http://hdl.handle.net/10355/44266.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Fereidouni, Mohammad 1. Evaluation of the coat protein of the Tombusviridae as HR elicitor in Nicotiana section. [Thesis]. University of Missouri – Columbia; 2014. Available from: http://hdl.handle.net/10355/44266

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Brno University of Technology

29. Kollner, Aleš. Evoluční řešení Rubikovy kostky .

Degree: 2010, Brno University of Technology

URL: http://hdl.handle.net/11012/54345

► Tato diplomová práce se zabývá problematikou řešení hlavolamu Rubikovy kostky. Popisuje hlavolam Rubikovy kostky s nejznámějšími metodami pro jeho složení. Hlavní náplní této práce je… (more)

Subjects/Keywords: Rubikova kostka; metoda vrstva za vrstvou; metoda Fridrich; evoluce; jednobodové křížení; uniformní křížení; mutace; proporcionální selekce; turnajová selekce; fitness funkce; evaluace; generace; jedinec.; Rubik's Cube; layer by layer method; Fridrich method; evolution; one-point crossover; uniform crossover; mutation; roulette wheel selection; tournament selection; fitness function; evaluation; generation; individual.

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Kollner, A. (2010). Evoluční řešení Rubikovy kostky . (Thesis). Brno University of Technology. Retrieved from http://hdl.handle.net/11012/54345

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Kollner, Aleš. “Evoluční řešení Rubikovy kostky .” 2010. Thesis, Brno University of Technology. Accessed December 12, 2019. http://hdl.handle.net/11012/54345.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Kollner, Aleš. “Evoluční řešení Rubikovy kostky .” 2010. Web. 12 Dec 2019.

Vancouver:

Kollner A. Evoluční řešení Rubikovy kostky . [Internet] [Thesis]. Brno University of Technology; 2010. [cited 2019 Dec 12]. Available from: http://hdl.handle.net/11012/54345.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Kollner A. Evoluční řešení Rubikovy kostky . [Thesis]. Brno University of Technology; 2010. Available from: http://hdl.handle.net/11012/54345

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Florida

30. Silvestri, Lynn Shiels, 1972-. Role of a conserved 3'NTR sequence and poly(A) tail length in poliovirus RNA replication.

Degree: 2001, University of Florida

URL: http://ufdc.ufl.edu/AA00026538

Subjects/Keywords: Gels; Genetic mutation; In vitro fertilization; Nucleotides; Point mutation; Poliovirus; Property reversion; RNA; RNA stability; Transfection; : Molecular Genetics and Microbiology thesis Ph.D ( mesh ); Conserved Sequence ( mesh ); Mutagenesis, Site-Directed ( mesh ); Polioviruses ( mesh ); RNA, Messenger – biosynthesis ( mesh ); Sequence Deletion ( mesh ); Virus Replication ( mesh )

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Silvestri, Lynn Shiels, 1. (2001). Role of a conserved 3'NTR sequence and poly(A) tail length in poliovirus RNA replication. (Thesis). University of Florida. Retrieved from http://ufdc.ufl.edu/AA00026538

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Silvestri, Lynn Shiels, 1972-. “Role of a conserved 3'NTR sequence and poly(A) tail length in poliovirus RNA replication.” 2001. Thesis, University of Florida. Accessed December 12, 2019. http://ufdc.ufl.edu/AA00026538.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Silvestri, Lynn Shiels, 1972-. “Role of a conserved 3'NTR sequence and poly(A) tail length in poliovirus RNA replication.” 2001. Web. 12 Dec 2019.

Vancouver:

Silvestri, Lynn Shiels 1. Role of a conserved 3'NTR sequence and poly(A) tail length in poliovirus RNA replication. [Internet] [Thesis]. University of Florida; 2001. [cited 2019 Dec 12]. Available from: http://ufdc.ufl.edu/AA00026538.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Silvestri, Lynn Shiels 1. Role of a conserved 3'NTR sequence and poly(A) tail length in poliovirus RNA replication. [Thesis]. University of Florida; 2001. Available from: http://ufdc.ufl.edu/AA00026538

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

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Open Access Theses and Dissertations