subject:(Plasmodium falciparum)

University of Zambia

1. Masase, Charles Chomba. Contribution of plasmodium falciparun and other infections to acute central nervous system disease at the University Teaching Hospital of Lusaka .

Degree: 2012, University of Zambia

URL: http://hdl.handle.net/123456789/1484

► Three hundred patients presenting with acute central nervous system (CNS) disease were enrolled in this one-year study from June 1997 to June 1998. This was… (more)

▼ Three hundred patients presenting with acute central nervous system (CNS) disease were enrolled in this one-year study from June 1997 to June 1998. This was a longitudinal prospective study looking at the contribution of Plasmodium falciparum and other infections to acute central nervous system disease. It also looked at the clinical and laboratory features of patients presenting with acute CNS disease. The clinical features were vast though most of the patients had a headache (84%), fever (70%) and confusion (40%). The other symptoms included convulsions (17%), weight loss (31%), diarrhoea (40%) while 34% of the patients had given a history of vomiting. The major signs were fever (87%), wasting (64%), meningeal signs (62%), lymphadenopathy (50%), papilloedema (19%), anaemia (54%) and 44% had non-specific macular popular dermatosis. The study has demonstrated that Plasmodium falciparum is an important cause of acute CNS diseases as 20% of the patients in this study had positive malaria parasite slides. The contribution of other infections were mainly bacteria and it has been shown that Cryptoccoccal neoformans (6.7%), Streptococcus pneumoniae (3.3%), Neisseria meningitides (2.7%), Haemophilus influenzae (0.7%), other bacteria (6.7%) are important causes of acute central nervous system disease. This study population, which had a 1:1 male to female ratio, had a high HIV seroprevalence rate of 75%. The biochemical results did not appear to be helpful except in situations were patients had hypoglycaemia. The RPR results were positive in only 3.3% of the study population. No acid alcohol bacilli were seen or isolated. The study has demonstrated that Plasmodium falciparum and other infections are an important cause of acute CNS diseases and should therefore always be considered in the management of affected individuals. The range of bacteria pathogens has increased with HIV related infections being common though there was limitation on the range of viral infections.

Subjects/Keywords: Plasmodium; Plasmodium Falciparum

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APA (6^th Edition):

Masase, C. C. (2012). Contribution of plasmodium falciparun and other infections to acute central nervous system disease at the University Teaching Hospital of Lusaka . (Thesis). University of Zambia. Retrieved from http://hdl.handle.net/123456789/1484

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Masase, Charles Chomba. “Contribution of plasmodium falciparun and other infections to acute central nervous system disease at the University Teaching Hospital of Lusaka .” 2012. Thesis, University of Zambia. Accessed February 26, 2021. http://hdl.handle.net/123456789/1484.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Masase, Charles Chomba. “Contribution of plasmodium falciparun and other infections to acute central nervous system disease at the University Teaching Hospital of Lusaka .” 2012. Web. 26 Feb 2021.

Vancouver:

Masase CC. Contribution of plasmodium falciparun and other infections to acute central nervous system disease at the University Teaching Hospital of Lusaka . [Internet] [Thesis]. University of Zambia; 2012. [cited 2021 Feb 26]. Available from: http://hdl.handle.net/123456789/1484.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Masase CC. Contribution of plasmodium falciparun and other infections to acute central nervous system disease at the University Teaching Hospital of Lusaka . [Thesis]. University of Zambia; 2012. Available from: http://hdl.handle.net/123456789/1484

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Ghana

2. Dawson-Amoah, M.E. The Genetic Diversity of Plasmodium Falciparum in Ghana .

Degree: 2018, University of Ghana

URL: http://ugspace.ug.edu.gh/handle/123456789/35244

► Background The population structure of the causative agents of human malaria, Plasmodium species including the most serious agent Plasmodium falciparum, depends on the local epidemiological… (more)

▼ Background The population structure of the causative agents of human malaria, Plasmodium species including the most serious agent Plasmodium falciparum, depends on the local epidemiological and demographic situations, such as the incidence of infected people, the vector transmission intensity and migration of inhabitants (i.e. exchange between sites).One of the major characteristics of malaria parasites is their genetic diversity and an increasing number of studies have reported on the population structure variation of P. falciparum. Genetic diversity and population structure of P. falciparum is required in predicting the origin and spread of novel variants within a population enabling specific malaria control measures in the era of intensive malaria intervention strategies. Aim This study investigated the genetic diversity of P. falciparum in sites with different malaria endemicity in Ghana. Methods Finger prick blood samples were made from primary school children in five study sites in Ghana. This was carried out during the dry (March) and rainy (July) seasons of 2017. The samples were collected on filter paper and on slides and examined for parasite detection using light microscopy (LM), and molecular diagnosis (18s rRNA nested PCR). The positive P. falciparum samples were characterized using microsatellite markers. Results: Light microscopy showed the highest prevalence in the Duase during the rainy season with 24.1%. Kpasolgu during the dry season sampling exhibited the lowest prevalence of P. falciparum parasites with 7.1%. The southern sites, Anyakpor and Odumase showed an intermediary prevalence of 12.8% and 16.9% respectively in the rainy season. This was the highest prevalence between the rainy and dry seasons in the southern sites. The sites Pagaza and Kpalsogu found in the northern part of Ghana, the Sahel savannah zone presented 17.7% and 18.2% prevalence respectively. This is according to light microscopy. Nested PCR detected a higher level of parasites in all samples. Duase in the Forest zone showed the highest prevalence, 58.8% in the samples collected in the rainy season. The Forest zone exhibited 9.8% indicating the lowest prevalence among the sites from the samples obtained in the dry season in Duase. This affirms that PCR is a more sensitive tool of detection of parasites in determining the parasite carriage. In determining the variation in diversity, genetic differentiations in the different sites tested were observed. There was a higher genetic diversity evidenced by the highest frequency of heterozygosity in the Northern region. Conclusion In this study, little significant differentiation in the genetic diversity was observed between the different ecological zones in Ghana. This reveals a range of population structures within the species of P. falciparum and suggests how genetic variation impacts the epidemiology and control measures between different transmission zones in Ghana.

Subjects/Keywords: Malaria; Plasmodium Species; Plasmodium Falciparum

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APA (6^th Edition):

Dawson-Amoah, M. E. (2018). The Genetic Diversity of Plasmodium Falciparum in Ghana . (Masters Thesis). University of Ghana. Retrieved from http://ugspace.ug.edu.gh/handle/123456789/35244

Chicago Manual of Style (16^th Edition):

Dawson-Amoah, M E. “The Genetic Diversity of Plasmodium Falciparum in Ghana .” 2018. Masters Thesis, University of Ghana. Accessed February 26, 2021. http://ugspace.ug.edu.gh/handle/123456789/35244.

MLA Handbook (7^th Edition):

Dawson-Amoah, M E. “The Genetic Diversity of Plasmodium Falciparum in Ghana .” 2018. Web. 26 Feb 2021.

Vancouver:

Dawson-Amoah ME. The Genetic Diversity of Plasmodium Falciparum in Ghana . [Internet] [Masters thesis]. University of Ghana; 2018. [cited 2021 Feb 26]. Available from: http://ugspace.ug.edu.gh/handle/123456789/35244.

Council of Science Editors:

Dawson-Amoah ME. The Genetic Diversity of Plasmodium Falciparum in Ghana . [Masters Thesis]. University of Ghana; 2018. Available from: http://ugspace.ug.edu.gh/handle/123456789/35244

3. ROSIMEIRE CRISTINA DALLA MARTHA. ANÁLISE DA VARIAÇÃO DE 11 MARCADORES DE MICROSSATÉLITES DE Plasmodium falciparum EM PACIENTES SINTOMÁTICOS E ASSINTOMÁTICOS DE UMA COMUNIDADE EM ZONA ENDEMICA DE MALÁRIA DE PORTO VELHO-RO.

Degree: 2005, Fundação Universidade Federal de Rondônia

URL: http://www.bdtd.unir.br/tde_busca/arquivo.php?codArquivo=29

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No presente trabalho foram estudados os genótipos de Plasmodium falciparum de indivíduos residentes em localidades distintas da cidade de Porto Velho (Vila de Candelária, Bate-… (more)

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No presente trabalho foram estudados os genótipos de Plasmodium falciparum de indivíduos residentes em localidades distintas da cidade de Porto Velho (Vila de Candelária, Bate- Estaca e diversos bairros da cidade). Quarenta e quatro amostras, divididas em cinco grupos: dois de indivíduos assintomáticos (em épocas de coletas diferentes e pertencentes da mesma localidade) e três sintomáticos (Vila de Candelária, Bate Estaca, e do atendimento do CEPEM), foram analisadas através de 11 marcadores de microssatélites. Para cada grupo distinto foi verificado o índice de diversidade, o Fst (índice de fixação) e a porcentagem de infecções múltiplas presentes em cada grupo e correlacionado com os demais. A presença de infecções múltiplas foi observada em todos os grupos estudados, ressaltando os pacientes assintomáticos, que também apresentaram maior número de alelos entre os demais grupos. O grupo Cepem obteve o maior índice de heterozigosidade, e entre os grupos sintomáticos e assintomáticos de uma mesma localidade, não se observa uma diferença considerável. O Fst entre os grupos assintomáticos de Candelária e sintomáticos de Bate Estaca (Fst=0.37) demonstraram maior diferenciação genética, apesar da proximidade geográfica das localidades. Este estudo confirma que não existem genótipos, caracterizados por determinadas combinações de tamanhos de microssatelites, que causam infecções sintomáticas ou assintomáticas. O motivo de uma infecção virar sintomática aparentemente está possivelmente mais associado à expressão diferencial de fatores associados a virulência.

In the present study, genotypes of Plasmodium falciparum parasites obtained from residents of different suburbs of Porto Velho were compared by means of microsatellite analysis. Fourty-four samples, divided in five groups were analyzed: two groups of samples from non-symptomatic infections (Candelaria, taken at different timepoints) and three groups of samples from symptomatic patients (from Candelaria, Bate-Estaca and CEPEM) were analyzed using eleven different microsatellite markers. For each distinct group, the diversity index, the fixation index (Fst-value) and the percentage of mixed (polyclonal) infections. All groups presented multiple infections to a similar degree. As expected, the group of samples from CEPEM showed the highest degree of heterozigosity, since samples were from patients of several different locations. There was no significant difference in heterozygosity between samples from asymptomatic and symptomatic infections of the same location. The largest genetic differentiation was observed between Candelária (asymptomatics) and Bate Estaca (symptomatics) samples, in spite of the geographical proximity of the two locations. This study confirms that the rather similar circulating Plasmodium falciparum genotypes, determined by microsatellite analysis, can either both cause symptomatic or asymptomatic infections and that the reason for this probably lies in the differential expression of virulence associated genes.

Advisors/Committee Members: Luiz Hildebrando Pereira da Silva.

Subjects/Keywords: BIOLOGIA GERAL; Plasmodium falciparum; Plasmodium falciparum

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APA (6^th Edition):

MARTHA, R. C. D. (2005). ANÁLISE DA VARIAÇÃO DE 11 MARCADORES DE MICROSSATÉLITES DE Plasmodium falciparum EM PACIENTES SINTOMÁTICOS E ASSINTOMÁTICOS DE UMA COMUNIDADE EM ZONA ENDEMICA DE MALÁRIA DE PORTO VELHO-RO. (Thesis). Fundação Universidade Federal de Rondônia. Retrieved from http://www.bdtd.unir.br/tde_busca/arquivo.php?codArquivo=29

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

MARTHA, ROSIMEIRE CRISTINA DALLA. “ANÁLISE DA VARIAÇÃO DE 11 MARCADORES DE MICROSSATÉLITES DE Plasmodium falciparum EM PACIENTES SINTOMÁTICOS E ASSINTOMÁTICOS DE UMA COMUNIDADE EM ZONA ENDEMICA DE MALÁRIA DE PORTO VELHO-RO.” 2005. Thesis, Fundação Universidade Federal de Rondônia. Accessed February 26, 2021. http://www.bdtd.unir.br/tde_busca/arquivo.php?codArquivo=29.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

MARTHA, ROSIMEIRE CRISTINA DALLA. “ANÁLISE DA VARIAÇÃO DE 11 MARCADORES DE MICROSSATÉLITES DE Plasmodium falciparum EM PACIENTES SINTOMÁTICOS E ASSINTOMÁTICOS DE UMA COMUNIDADE EM ZONA ENDEMICA DE MALÁRIA DE PORTO VELHO-RO.” 2005. Web. 26 Feb 2021.

Vancouver:

MARTHA RCD. ANÁLISE DA VARIAÇÃO DE 11 MARCADORES DE MICROSSATÉLITES DE Plasmodium falciparum EM PACIENTES SINTOMÁTICOS E ASSINTOMÁTICOS DE UMA COMUNIDADE EM ZONA ENDEMICA DE MALÁRIA DE PORTO VELHO-RO. [Internet] [Thesis]. Fundação Universidade Federal de Rondônia; 2005. [cited 2021 Feb 26]. Available from: http://www.bdtd.unir.br/tde_busca/arquivo.php?codArquivo=29.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

MARTHA RCD. ANÁLISE DA VARIAÇÃO DE 11 MARCADORES DE MICROSSATÉLITES DE Plasmodium falciparum EM PACIENTES SINTOMÁTICOS E ASSINTOMÁTICOS DE UMA COMUNIDADE EM ZONA ENDEMICA DE MALÁRIA DE PORTO VELHO-RO. [Thesis]. Fundação Universidade Federal de Rondônia; 2005. Available from: http://www.bdtd.unir.br/tde_busca/arquivo.php?codArquivo=29

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Zambia

4. Hawela, Moonga B. Determination of plasmodium falciparum parasites diversity using MSPII family specific allelic primers among symptomatic children aged five years and below in Zambia .

Degree: 2012, University of Zambia

URL: http://hdl.handle.net/123456789/1320

► Introduction: Malaria is still a major public health problem in many tropical and subtropical countries. Therefore effective integrated malaria control strategies are a requirement. However,… (more)

▼ Introduction: Malaria is still a major public health problem in many tropical and subtropical countries. Therefore effective integrated malaria control strategies are a requirement. However, parasite antigen diversity might be a hurdle in the efficacy of some malaria control strategies like the malaria vaccine against Plasmodium falciparum infection if a full repertoire of variant forms is not incorporated into the vaccine. In addition, malaria rapid diagnostic tests (RDTs) offer great potential for rapid and accurate diagnosis of malaria infections. Parasite genetic diversity of target antigens particularly for PfEiRP2-based RDTs may affect their sensitivity among other factors. Methods: In 2006, a prospective analytical study was conducted to determine the diversity oi Plasmodium falciparum merozoite surface protein 2 (MSP-2) in four sites in Zambia. A nested polymerase chain reaction (PCR) amplification was carried out on two hundred and eighty five dried blood spots with Plasmodium falciparum mono infection using primers for the conserved regions of MSP-2 as well as family specific alleles for the variable regions. PCR-restriction fragment length polymorphism analysis of MSP-2 using a Hinfl digests of each nested PCR product followed by product detection on a 2.0% agarose gel electrophoresis with ethidium bromide was performed to determine the allelic families and genotypes. Results: The study findings show that, there are forty six different genotypes of Plasmodium falciparum commonly found in Zambia, twenty four allelic genotypes belong to the FC27 allelic family and twenty two fi-om the 3D7 allelic family. The study also indicated that Plasmodium falciparum infections carry more than one allelic genotype at each particular time and one infection carried eight different genotypes. Discussion: These findings show that Plasmodium falciparum in Zambia exists in many different allelic genotypes and several patients infected with Plasmodium falciparum carry atleast more than one genotype. Conclusions: This means that the inclusion of all the available genotypes in both the Malaria vaccine and RDTs would be of maximum benefit to the Zambian populations. The multiple Plasmodium falciparum infections will have other implications such as having one genotype sensitive the drug and another resistant therefore this needs to be further explored.

Subjects/Keywords: Plasmodium falciparum; Malaria

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Hawela, M. B. (2012). Determination of plasmodium falciparum parasites diversity using MSPII family specific allelic primers among symptomatic children aged five years and below in Zambia . (Thesis). University of Zambia. Retrieved from http://hdl.handle.net/123456789/1320

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Hawela, Moonga B. “Determination of plasmodium falciparum parasites diversity using MSPII family specific allelic primers among symptomatic children aged five years and below in Zambia .” 2012. Thesis, University of Zambia. Accessed February 26, 2021. http://hdl.handle.net/123456789/1320.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Hawela, Moonga B. “Determination of plasmodium falciparum parasites diversity using MSPII family specific allelic primers among symptomatic children aged five years and below in Zambia .” 2012. Web. 26 Feb 2021.

Vancouver:

Hawela MB. Determination of plasmodium falciparum parasites diversity using MSPII family specific allelic primers among symptomatic children aged five years and below in Zambia . [Internet] [Thesis]. University of Zambia; 2012. [cited 2021 Feb 26]. Available from: http://hdl.handle.net/123456789/1320.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Hawela MB. Determination of plasmodium falciparum parasites diversity using MSPII family specific allelic primers among symptomatic children aged five years and below in Zambia . [Thesis]. University of Zambia; 2012. Available from: http://hdl.handle.net/123456789/1320

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Latrobe University

5. Abu Bakar, Nurhidanatasha. Endocytic and digestive processes in Plasmodium falciparum and their role in artemisinin action.

Degree: PhD, 2011, Latrobe University

URL: http://hdl.handle.net/1959.9/492830

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Thesis (Ph.D.) - La Trobe University, 2011

Submission note: "A thesis submitted in total fulfilment of the requirements for the degree of Doctor of Philosophy,… (more)

Subjects/Keywords: Plasmodium falciparum.; Artemisinin.

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APA (6^th Edition):

Abu Bakar, N. (2011). Endocytic and digestive processes in Plasmodium falciparum and their role in artemisinin action. (Doctoral Dissertation). Latrobe University. Retrieved from http://hdl.handle.net/1959.9/492830

Chicago Manual of Style (16^th Edition):

Abu Bakar, Nurhidanatasha. “Endocytic and digestive processes in Plasmodium falciparum and their role in artemisinin action.” 2011. Doctoral Dissertation, Latrobe University. Accessed February 26, 2021. http://hdl.handle.net/1959.9/492830.

MLA Handbook (7^th Edition):

Abu Bakar, Nurhidanatasha. “Endocytic and digestive processes in Plasmodium falciparum and their role in artemisinin action.” 2011. Web. 26 Feb 2021.

Vancouver:

Abu Bakar N. Endocytic and digestive processes in Plasmodium falciparum and their role in artemisinin action. [Internet] [Doctoral dissertation]. Latrobe University; 2011. [cited 2021 Feb 26]. Available from: http://hdl.handle.net/1959.9/492830.

Council of Science Editors:

Abu Bakar N. Endocytic and digestive processes in Plasmodium falciparum and their role in artemisinin action. [Doctoral Dissertation]. Latrobe University; 2011. Available from: http://hdl.handle.net/1959.9/492830

University of Pretoria

6. Burger, Pieter Buys. Development of a dynamic receptor-based pharmacophore model of Plasmodium falciparum spermidine synthase for selective inhibitor identification .

Degree: 2009, University of Pretoria

URL: http://upetd.up.ac.za/thesis/available/etd-05252009-220942/

► Malaria affects the daily lives of more than 2 billion people worldwide and has been estimated to result in 300-500 million clinical cases annually leading… (more)

▼ Malaria affects the daily lives of more than 2 billion people worldwide and has been estimated to result in 300-500 million clinical cases annually leading to approximately 2 million deaths, mainly caused by the most virulent malaria species, Plasmodium falciparum. The lack of a vaccine and the rapid emergence and spread of drug resistant strains of P. falciparum, necessitate the development of new antimalarials and the identification and validation of new parasite-specific therapeutic targets. Numerous studies directed at interfering with the polyamine biosynthetic pathway in P. falciparum have shown its potential as a target for the development of a new class of antimalarials. The essential nature of P. falciparum spermidine synthase (PfSpdSyn), an enzyme in the polyamine pathway of the parasite warranted the further investigation to find novel lead compounds. The high cost and attrition rate of drug discovery has resulted in the implementation of smart drug discovery platforms in both academia and industry. The strategy implemented in this study involved the development of a dynamic receptor-based pharmacophore model (DPM) of PfSpdSyn complemented by a knowledge-based rational design strategy. The use of pharmacophore models to identify lead compounds has become increasingly popular over the last decade and has been shown to be a reliable method in the drug discovery process. The development of a DPM allows for the incorporation of protein exibility within the drug design process. This methodology results in a wealth of information of the chemical space of the active site and was incorporated in designing new inhibitors against PfSpdSyn using a knowledge-based rational design strategy. The active site of PfSpdSyn was subdivided into four binding regions (DPM1-DPM4) to allow for the identi cation of fragments binding within these speci c binding regions. DPMs representative of the chemical characteristics of each binding region were constructed and subsequently screened against the drug-like subset of the ZINC database. From the screens a total of nine compounds were selected for in vitro testing, complementing each other in exploring specific active site binding characteristics. From these compounds a new lead compound N-(3-aminopropyl)-cyclohexylamine (NAC; Ki 2.8 μM) was identified for PfSpdSyn. NAC was specifically designed to bind in both the putrescine and decarboxylated adenosylmethionine cavities by chemically bridging the catalytic center and was confirmed by kinetic studies. NAC shows great potential for lead optimization to increase its binding affinity. This study then paves the way for lead optimization and possibly the development of a novel antimalarial. The development of a DPM for PfSpdSyn has seen the establishment of this methodology in the Bioinformatics and Computational Biology Unit, Department of Biochemistry at the University of Pretoria. It can be concluded that the development of a DPM complemented by a knowledge-based rational design strategy is an effective approach for the… Advisors/Committee Members: Prof A I Louw (advisor), Dr L M Birkholtz (advisor), Prof F Joubert (advisor), Prof J M Briggs (advisor).

Subjects/Keywords: Spermidine synthase; Plasmodium falciparum; UCTD

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APA (6^th Edition):

Burger, P. B. (2009). Development of a dynamic receptor-based pharmacophore model of Plasmodium falciparum spermidine synthase for selective inhibitor identification . (Doctoral Dissertation). University of Pretoria. Retrieved from http://upetd.up.ac.za/thesis/available/etd-05252009-220942/

Chicago Manual of Style (16^th Edition):

Burger, Pieter Buys. “Development of a dynamic receptor-based pharmacophore model of Plasmodium falciparum spermidine synthase for selective inhibitor identification .” 2009. Doctoral Dissertation, University of Pretoria. Accessed February 26, 2021. http://upetd.up.ac.za/thesis/available/etd-05252009-220942/.

MLA Handbook (7^th Edition):

Burger, Pieter Buys. “Development of a dynamic receptor-based pharmacophore model of Plasmodium falciparum spermidine synthase for selective inhibitor identification .” 2009. Web. 26 Feb 2021.

Vancouver:

Burger PB. Development of a dynamic receptor-based pharmacophore model of Plasmodium falciparum spermidine synthase for selective inhibitor identification . [Internet] [Doctoral dissertation]. University of Pretoria; 2009. [cited 2021 Feb 26]. Available from: http://upetd.up.ac.za/thesis/available/etd-05252009-220942/.

Council of Science Editors:

Burger PB. Development of a dynamic receptor-based pharmacophore model of Plasmodium falciparum spermidine synthase for selective inhibitor identification . [Doctoral Dissertation]. University of Pretoria; 2009. Available from: http://upetd.up.ac.za/thesis/available/etd-05252009-220942/

University of Pretoria

7. [No author]. Flow cytometric evaluation of riminophenazines as antimalarial agents .

Degree: 2010, University of Pretoria

URL: http://upetd.up.ac.za/thesis/available/etd-09202010-113318/

► The in vitro antimalarial activity of clofazimine and seven of its analogues, all TMP(tetramethyl-piperidyl group)-derivatives except 8669, against the R8-1 and pfUP-1 laboratory strains of… (more)

Subjects/Keywords: Plasmodium falciparum; Clofazimine; Antimalarial; UCTD

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APA (6^th Edition):

author], [. (2010). Flow cytometric evaluation of riminophenazines as antimalarial agents . (Masters Thesis). University of Pretoria. Retrieved from http://upetd.up.ac.za/thesis/available/etd-09202010-113318/

Chicago Manual of Style (16^th Edition):

author], [No. “Flow cytometric evaluation of riminophenazines as antimalarial agents .” 2010. Masters Thesis, University of Pretoria. Accessed February 26, 2021. http://upetd.up.ac.za/thesis/available/etd-09202010-113318/.

MLA Handbook (7^th Edition):

author], [No. “Flow cytometric evaluation of riminophenazines as antimalarial agents .” 2010. Web. 26 Feb 2021.

Vancouver:

author] [. Flow cytometric evaluation of riminophenazines as antimalarial agents . [Internet] [Masters thesis]. University of Pretoria; 2010. [cited 2021 Feb 26]. Available from: http://upetd.up.ac.za/thesis/available/etd-09202010-113318/.

Council of Science Editors:

author] [. Flow cytometric evaluation of riminophenazines as antimalarial agents . [Masters Thesis]. University of Pretoria; 2010. Available from: http://upetd.up.ac.za/thesis/available/etd-09202010-113318/

University of Pretoria

8. Burger, Pieter Buys. Development of a dynamic receptor-based pharmacophore model of Plasmodium falciparum spermidine synthase for selective inhibitor identification.

Degree: Biochemistry, 2009, University of Pretoria

URL: http://hdl.handle.net/2263/24974

► Malaria affects the daily lives of more than 2 billion people worldwide and has been estimated to result in 300-500 million clinical cases annually leading… (more)

▼ Malaria affects the daily lives of more than 2 billion people worldwide and has been estimated to result in 300-500 million clinical cases annually leading to approximately 2 million deaths, mainly caused by the most virulent malaria species, Plasmodium falciparum. The lack of a vaccine and the rapid emergence and spread of drug resistant strains of P. falciparum, necessitate the development of new antimalarials and the identification and validation of new parasite-specific therapeutic targets. Numerous studies directed at interfering with the polyamine biosynthetic pathway in P. falciparum have shown its potential as a target for the development of a new class of antimalarials. The essential nature of P. falciparum spermidine synthase (PfSpdSyn), an enzyme in the polyamine pathway of the parasite warranted the further investigation to find novel lead compounds. The high cost and attrition rate of drug discovery has resulted in the implementation of smart drug discovery platforms in both academia and industry. The strategy implemented in this study involved the development of a dynamic receptor-based pharmacophore model (DPM) of PfSpdSyn complemented by a knowledge-based rational design strategy. The use of pharmacophore models to identify lead compounds has become increasingly popular over the last decade and has been shown to be a reliable method in the drug discovery process. The development of a DPM allows for the incorporation of protein exibility within the drug design process. This methodology results in a wealth of information of the chemical space of the active site and was incorporated in designing new inhibitors against PfSpdSyn using a knowledge-based rational design strategy. The active site of PfSpdSyn was subdivided into four binding regions (DPM1-DPM4) to allow for the identi cation of fragments binding within these speci c binding regions. DPMs representative of the chemical characteristics of each binding region were constructed and subsequently screened against the drug-like subset of the ZINC database. From the screens a total of nine compounds were selected for in vitro testing, complementing each other in exploring specific active site binding characteristics. From these compounds a new lead compound N-(3-aminopropyl)-cyclohexylamine (NAC; Ki 2.8 μM) was identified for PfSpdSyn. NAC was specifically designed to bind in both the putrescine and decarboxylated adenosylmethionine cavities by chemically bridging the catalytic center and was confirmed by kinetic studies. NAC shows great potential for lead optimization to increase its binding affinity. This study then paves the way for lead optimization and possibly the development of a novel antimalarial. The development of a DPM for PfSpdSyn has seen the establishment of this methodology in the Bioinformatics and Computational Biology Unit, Department of Biochemistry at the University of Pretoria. It can be concluded that the development of a DPM complemented by a knowledge-based rational design strategy is an effective approach for the… Advisors/Committee Members: Prof A I Louw (advisor), Dr L M Birkholtz (advisor), Prof F Joubert (advisor), Prof J M Briggs (advisor).

Subjects/Keywords: Spermidine synthase; Plasmodium falciparum; UCTD

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APA (6^th Edition):

Burger, P. B. (2009). Development of a dynamic receptor-based pharmacophore model of Plasmodium falciparum spermidine synthase for selective inhibitor identification. (Doctoral Dissertation). University of Pretoria. Retrieved from http://hdl.handle.net/2263/24974

Chicago Manual of Style (16^th Edition):

Burger, Pieter Buys. “Development of a dynamic receptor-based pharmacophore model of Plasmodium falciparum spermidine synthase for selective inhibitor identification.” 2009. Doctoral Dissertation, University of Pretoria. Accessed February 26, 2021. http://hdl.handle.net/2263/24974.

MLA Handbook (7^th Edition):

Burger, Pieter Buys. “Development of a dynamic receptor-based pharmacophore model of Plasmodium falciparum spermidine synthase for selective inhibitor identification.” 2009. Web. 26 Feb 2021.

Vancouver:

Burger PB. Development of a dynamic receptor-based pharmacophore model of Plasmodium falciparum spermidine synthase for selective inhibitor identification. [Internet] [Doctoral dissertation]. University of Pretoria; 2009. [cited 2021 Feb 26]. Available from: http://hdl.handle.net/2263/24974.

Council of Science Editors:

Burger PB. Development of a dynamic receptor-based pharmacophore model of Plasmodium falciparum spermidine synthase for selective inhibitor identification. [Doctoral Dissertation]. University of Pretoria; 2009. Available from: http://hdl.handle.net/2263/24974

9. Majeau, Alicia Catherine. INVESTIGATING DIFFERENTIAL ATTRACTIVENESS OF HUMAN SKIN MICROBIOTA TO ANOPHELES GAMBIAE AS A POTENTIAL COMPONENT OF BAITED SUGAR TRAPS CONTAINING ANTI-PLASMODIUM BACTERIA FOR BIOLOGICAL CONTROL.

Degree: 2014, Johns Hopkins University

URL: http://jhir.library.jhu.edu/handle/1774.2/37217

► Malaria is a pressing global health problem that is difficult to eradicate or even control because of its complex biology. Currently employed control mechanisms are… (more)

Subjects/Keywords: Plasmodium falciparum; olfaction; skin microbiota

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APA (6^th Edition):

Majeau, A. C. (2014). INVESTIGATING DIFFERENTIAL ATTRACTIVENESS OF HUMAN SKIN MICROBIOTA TO ANOPHELES GAMBIAE AS A POTENTIAL COMPONENT OF BAITED SUGAR TRAPS CONTAINING ANTI-PLASMODIUM BACTERIA FOR BIOLOGICAL CONTROL. (Thesis). Johns Hopkins University. Retrieved from http://jhir.library.jhu.edu/handle/1774.2/37217

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Majeau, Alicia Catherine. “INVESTIGATING DIFFERENTIAL ATTRACTIVENESS OF HUMAN SKIN MICROBIOTA TO ANOPHELES GAMBIAE AS A POTENTIAL COMPONENT OF BAITED SUGAR TRAPS CONTAINING ANTI-PLASMODIUM BACTERIA FOR BIOLOGICAL CONTROL.” 2014. Thesis, Johns Hopkins University. Accessed February 26, 2021. http://jhir.library.jhu.edu/handle/1774.2/37217.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Majeau, Alicia Catherine. “INVESTIGATING DIFFERENTIAL ATTRACTIVENESS OF HUMAN SKIN MICROBIOTA TO ANOPHELES GAMBIAE AS A POTENTIAL COMPONENT OF BAITED SUGAR TRAPS CONTAINING ANTI-PLASMODIUM BACTERIA FOR BIOLOGICAL CONTROL.” 2014. Web. 26 Feb 2021.

Vancouver:

Majeau AC. INVESTIGATING DIFFERENTIAL ATTRACTIVENESS OF HUMAN SKIN MICROBIOTA TO ANOPHELES GAMBIAE AS A POTENTIAL COMPONENT OF BAITED SUGAR TRAPS CONTAINING ANTI-PLASMODIUM BACTERIA FOR BIOLOGICAL CONTROL. [Internet] [Thesis]. Johns Hopkins University; 2014. [cited 2021 Feb 26]. Available from: http://jhir.library.jhu.edu/handle/1774.2/37217.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Majeau AC. INVESTIGATING DIFFERENTIAL ATTRACTIVENESS OF HUMAN SKIN MICROBIOTA TO ANOPHELES GAMBIAE AS A POTENTIAL COMPONENT OF BAITED SUGAR TRAPS CONTAINING ANTI-PLASMODIUM BACTERIA FOR BIOLOGICAL CONTROL. [Thesis]. Johns Hopkins University; 2014. Available from: http://jhir.library.jhu.edu/handle/1774.2/37217

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

10. Sonia Devi Lourembam. Protective immune response in plasmodium falciparum malaria: a molecular immunoepidemiological study; -.

Degree: Biotechnology, 2011, Tezpur University

URL: http://shodhganga.inflibnet.ac.in/handle/10603/9014

►

Malaria is a major health problem with the disease burden mostly borne by economically productive ages. Recent studies indicate the disease is becoming more widespread… (more)

▼

Malaria is a major health problem with the disease burden mostly borne by economically productive ages. Recent studies indicate the disease is becoming more widespread in south east Asia with 10 of the 11 countries endemic for malaria and with 1.2 billion people at risk of exposure to the disease. India reported the highest malaria confirmed (1563344) and death (1133) cases in 2009 from this region (WHO). The north-eastern region of India (population 28.5 million) is highly endemic for malaria and has been declared as a high risk zone by National Anti Malaria Program. Assam contributes to 64.7% of the malaria positive cases in the north-eastern region of which P. falciparum accounts for 58-75% of the cases and the remainder are due to Plasmodium vivax. Though people living in endemic area develop immunity, which can be either anti disease or anti infection immunity, the factors delineating them are not clear. It is thus felt that an in depth understanding of the immune response leading to anti infection or to anti disease responses in population genetic studies and elucidation of parasite diversity is required for success of malaria control programs. The present study was designed against this perspective with the following objectives: 1) To study the clone multiplicity of the parasite genotypes existing in the study area 2) To elucidate the protective humoral immune response. 3) To elucidate the protective cell mediated immune response. The study was conducted at Guabari village of Baksa district and Kondoli Tea Estate (KTE) of Nagaon district which are mesoendemic for malaria. The two sites differ in population demography and accessibility to health care. Plasmodium falciparum diversity was assessed by typing polymorphic block 2 region of Merozoite Surface Protein 1(MSP-1) gene using primary as well as nested PCR cycles. A high degree of polymorphism in isolates from the two study sites was seen with 33 alleles of MSP-1 seen in Guabari village and 18 alleles at KTE.

References p.143-164, Abstract given chapter wise

Advisors/Committee Members: Baruah, S.

Subjects/Keywords: Biology; Plasmodium falciparum; Malaria; Cytokines

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APA (6^th Edition):

Lourembam, S. D. (2011). Protective immune response in plasmodium falciparum malaria: a molecular immunoepidemiological study; -. (Thesis). Tezpur University. Retrieved from http://shodhganga.inflibnet.ac.in/handle/10603/9014

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Lourembam, Sonia Devi. “Protective immune response in plasmodium falciparum malaria: a molecular immunoepidemiological study; -.” 2011. Thesis, Tezpur University. Accessed February 26, 2021. http://shodhganga.inflibnet.ac.in/handle/10603/9014.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Lourembam, Sonia Devi. “Protective immune response in plasmodium falciparum malaria: a molecular immunoepidemiological study; -.” 2011. Web. 26 Feb 2021.

Vancouver:

Lourembam SD. Protective immune response in plasmodium falciparum malaria: a molecular immunoepidemiological study; -. [Internet] [Thesis]. Tezpur University; 2011. [cited 2021 Feb 26]. Available from: http://shodhganga.inflibnet.ac.in/handle/10603/9014.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Lourembam SD. Protective immune response in plasmodium falciparum malaria: a molecular immunoepidemiological study; -. [Thesis]. Tezpur University; 2011. Available from: http://shodhganga.inflibnet.ac.in/handle/10603/9014

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Pretoria

11. Makgatho, Ephraim Marema. Flow cytometric evaluation of riminophenazines as antimalarial agents.

Degree: Pharmacology, 2010, University of Pretoria

URL: http://hdl.handle.net/2263/28057

► The in vitro antimalarial activity of clofazimine and seven of its analogues, all TMP(tetramethyl-piperidyl group)-derivatives except 8669, against the R8-1 and pfUP-1 laboratory strains of… (more)

Subjects/Keywords: Plasmodium falciparum; Clofazimine; Antimalarial; UCTD

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Makgatho, E. (2010). Flow cytometric evaluation of riminophenazines as antimalarial agents. (Masters Thesis). University of Pretoria. Retrieved from http://hdl.handle.net/2263/28057

Chicago Manual of Style (16^th Edition):

Makgatho, Ephraim. “Flow cytometric evaluation of riminophenazines as antimalarial agents.” 2010. Masters Thesis, University of Pretoria. Accessed February 26, 2021. http://hdl.handle.net/2263/28057.

MLA Handbook (7^th Edition):

Makgatho, Ephraim. “Flow cytometric evaluation of riminophenazines as antimalarial agents.” 2010. Web. 26 Feb 2021.

Vancouver:

Makgatho E. Flow cytometric evaluation of riminophenazines as antimalarial agents. [Internet] [Masters thesis]. University of Pretoria; 2010. [cited 2021 Feb 26]. Available from: http://hdl.handle.net/2263/28057.

Council of Science Editors:

Makgatho E. Flow cytometric evaluation of riminophenazines as antimalarial agents. [Masters Thesis]. University of Pretoria; 2010. Available from: http://hdl.handle.net/2263/28057

Harvard University

12. Ravel, Deepali B. Mechanisms of Cellular Remodeling during Plasmodium falciparum Sequestration and Transmission.

Degree: PhD, 2017, Harvard University

URL: http://nrs.harvard.edu/urn-3:HUL.InstRepos:42061497

►

Despite significant progress in global malaria control, Plasmodium falciparum remains a major cause of morbidity and mortality world-wide. The need for new interventions demands better… (more)

▼

Despite significant progress in global malaria control, Plasmodium falciparum remains a major cause of morbidity and mortality world-wide. The need for new interventions demands better understanding of the underlying cellular mechanisms that mediate parasite survival and transmission. Asexual growth and the development of sexual stages, termed gametocytes, take place in human red blood cells, and sexual reproduction takes place in the mosquito vector. Blood stage parasites sequester in host tissues to survive, requiring extensive cellular remodeling. Sequestering asexual stage parasites export hundreds of proteins into the host cell to change its deformability and cytoadherence. Developing gametocytes also sequester in deep tissues before reentering circulation to mediate transmission, and they too are characterized by protein export, deformability changes, and likely cytoadherence. Although significant progress has been made in understanding remodeling changes that occur to mediate sequestration and transmission, many open questions remain. In this work, we identified and characterized proteins involved in cellular remodeling of asexual and sexual stage parasites. We first investigated red blood cell remodeling and sequestration during blood stage development, focusing specifically on the Plasmodium helical interspersed sub-telomeric c (PHISTc) protein family. Using a combination of biochemistry, biophysics, flow cytometry, and microscopy, we showed that PHISTc proteins are exported to the host cell of asexual and sexual stages and are required for the delivery of specific antigens to the surface of asexual stage infected red blood cells. Further, they play this specialized role in antigen delivery without drastically altering cellular architecture. Next, we investigated the mechanisms that drive the transition of stiff, sequestered gametocytes into deformable, circulating gametocytes. Using biophysical and transcriptional approaches, we identified genes that are enriched or depleted in deformable gametocytes. Some of these candidate genes likely remodel the host red blood cell while others may remodel the parasite itself, with some candidates required for the formation of deformable, infectious gametocytes. We anticipate that these genes either drive or delineate the deformability transition and could represent possible targets of therapeutics or diagnostics used to reduce malaria transmission. Together, these studies reveal both conserved and stage-specific mechanisms involved in the successful sequestration and transmission of Plasmodium falciparum.

Medical Sciences

Advisors/Committee Members: Wirth, Dyann (committee member), Dvorin, Jeffrey (committee member), Goldberg, Daniel (committee member).

Subjects/Keywords: Plasmodium falciparum; cellular remodeling

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Ravel, D. B. (2017). Mechanisms of Cellular Remodeling during Plasmodium falciparum Sequestration and Transmission. (Doctoral Dissertation). Harvard University. Retrieved from http://nrs.harvard.edu/urn-3:HUL.InstRepos:42061497

Chicago Manual of Style (16^th Edition):

Ravel, Deepali B. “Mechanisms of Cellular Remodeling during Plasmodium falciparum Sequestration and Transmission.” 2017. Doctoral Dissertation, Harvard University. Accessed February 26, 2021. http://nrs.harvard.edu/urn-3:HUL.InstRepos:42061497.

MLA Handbook (7^th Edition):

Ravel, Deepali B. “Mechanisms of Cellular Remodeling during Plasmodium falciparum Sequestration and Transmission.” 2017. Web. 26 Feb 2021.

Vancouver:

Ravel DB. Mechanisms of Cellular Remodeling during Plasmodium falciparum Sequestration and Transmission. [Internet] [Doctoral dissertation]. Harvard University; 2017. [cited 2021 Feb 26]. Available from: http://nrs.harvard.edu/urn-3:HUL.InstRepos:42061497.

Council of Science Editors:

Ravel DB. Mechanisms of Cellular Remodeling during Plasmodium falciparum Sequestration and Transmission. [Doctoral Dissertation]. Harvard University; 2017. Available from: http://nrs.harvard.edu/urn-3:HUL.InstRepos:42061497

13. Mwenechanya, Roy. In-vivo and Molecular characterization of drug resistant plasmodium falciparum isolates from Solwezi, Zambia.

Degree: 2011, University of Zimbabwe

URL: http://dspace.unza.zm/handle/123456789/283

► The study assessed the prevalence of drug resistant malarial parasites in patients at Solwezi urban clinic in northwestern province of Zambia in February 2004, using… (more)

▼ The study assessed the prevalence of drug resistant malarial parasites in patients at Solwezi urban clinic in northwestern province of Zambia in February 2004, using the in-vivo and molecular methods. In the in-vivo method each patient was monitored for signs and symptoms of the disease following treatment with either sulfadoxine/pyrimethamine (fansidar®), lumefantrine/artemether (coartem®) or quinine. Molecular determination of mutations on the Plasmodium falciparum chloroquine resistance transporter gene (pfcrt), associated with chloroquine resistance, and the dihydropteroate synthase gene (dhps) and the dihydrofolate reductase gene (dhfr), associated with sulfadoxine and pyrimethamine resistance, respectively, was also done on parasites obtained from the same patients, before treatment, on the day of recruitment to the study. The extracted Plasmodium DNA was amplified using nested PCR and the resulting secondary PCR products were digested with restriction enzymes. The digested DNA fragments were separated by electrophoresis using 2 - 2.5% agarose gels and visualized by using UV transillumination after staining with ethidium bromide. In-vivo analysis revealed a 27% (n=74) fansidar® treatment failure rate. The molecular analysis revealed 11% (n=108) prevalence of the combination mutation at codons 437 and 108 of the dhps and dhfr, respectively, associated with fansidar® resistance. There was a significant association between the presence of a combination of mutations on both genes and the in-vivo S/P treatment response in patients (x , P = 0.001). There was, however, no significant association between the in-vivo response in patients that received sulfadoxine/pyrimethamine treatment and the presence of a single or combined mutation(s) on either the dhps (codon 437: x2, P = 0.143; codon 540: x2, P = 0.205; codon 437/540: x2, P = 0.454) or the dhfr (codon 108: x2, P = 0.390; codon 59: x2, P = 0.246; codons 108/59: x2, P = 0.969) alone. Molecular analysis showed a 97% (152) prevalence of the pre-requisite mutation K76T, for chloroquine resistance while the supporting mutation at codon 75 had a prevalence of 99% (n=155). The higher in-vivo treatment failure rate in comparison with the lower prevalence of mutation associated with resistance recorded could be attributed to other causes other than the presence of drug resistant parasites. The study demonstrated that the collection of P. falciparum infected blood samples on filter papers and the use of restriction fragment length polymorphism molecular analysis is a viable method that can be employed in the identification of drug resistant Plasmodium isolates.

Subjects/Keywords: Plasmodium falciparum – Solwezi – Zambia

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APA (6^th Edition):

Mwenechanya, R. (2011). In-vivo and Molecular characterization of drug resistant plasmodium falciparum isolates from Solwezi, Zambia. (Thesis). University of Zimbabwe. Retrieved from http://dspace.unza.zm/handle/123456789/283

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Mwenechanya, Roy. “In-vivo and Molecular characterization of drug resistant plasmodium falciparum isolates from Solwezi, Zambia.” 2011. Thesis, University of Zimbabwe. Accessed February 26, 2021. http://dspace.unza.zm/handle/123456789/283.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Mwenechanya, Roy. “In-vivo and Molecular characterization of drug resistant plasmodium falciparum isolates from Solwezi, Zambia.” 2011. Web. 26 Feb 2021.

Vancouver:

Mwenechanya R. In-vivo and Molecular characterization of drug resistant plasmodium falciparum isolates from Solwezi, Zambia. [Internet] [Thesis]. University of Zimbabwe; 2011. [cited 2021 Feb 26]. Available from: http://dspace.unza.zm/handle/123456789/283.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Mwenechanya R. In-vivo and Molecular characterization of drug resistant plasmodium falciparum isolates from Solwezi, Zambia. [Thesis]. University of Zimbabwe; 2011. Available from: http://dspace.unza.zm/handle/123456789/283

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Université Laval

14. Ebrahimzadeh, Zeinab. Exploring the roles of phosphoinositides in the biology of the malaria parasite Plasmodium falciparum.

Degree: 2019, Université Laval

URL: http://hdl.handle.net/20.500.11794/37075

► Plasmodium falciparum est un parasite appartenant au phylum Apicomplexa et est à l’origine de la forme la plus sévère de la malaria. Dans les zones… (more)

▼ Plasmodium falciparum est un parasite appartenant au phylum Apicomplexa et est à l’origine de la forme la plus sévère de la malaria. Dans les zones endémiques d'Afrique subsaharienne, la plupart des victimes sont des enfants de moins de cinq ans. L’entrée de P. falciparum dans sa cellule cible, le globule rouge, repose sur la sécrétion de protéines par des organites spécialisés : les micronèmes, les rhoptries et les granules denses. Les mécanismes de biogenèse de ces organites et la coordination de la libération de leur contenu lors de l'invasion sont cependant pour la plupart inconnus. Il a été toutefois été démontré que les protéines destinées à ces organites apicaux se concentrent dans des microdomaines de l’appareil de Golgi, dont la composition en lipides et en protéines détermine leur destination finale. À ce jour, les mécanismes de sélection et de transport des protéines apicales vers les organites d'invasion ainsi que leurs mécanismes de sécrétion durant l’invasion sont pour la plupart inconnus. Nous avons donc posé l’hypothèse que les phosphoinositides (PI) et leurs protéines effectrices sont impliqués dans ces processus chez P. falciparum. Les PI sont sept lipides phosphorylés retrouvés de façon minoritaire dans les différentes membranes cellulaires. Chaque membrane subcellulaire contient une espèce caractéristique de PI qui peut être reconnue et liée spécifiquement par des protéines effectrices. Une large gamme de processus biologiques sont régulés par les PI, tels le trafic vésiculaire, les canaux ioniques, les pompes d’efflux et les transporteurs, ainsi que certains processus endocytiques et exocytaires. Des études antérieures ont été en mesure de détecter seulement cinq des sept espèces de PI chez P. falciparum. Dans le cadre d’un premier projet, nous avons étudié la distribution de six PI, à savoir PI3P, PI4P, PI5P, PI (4,5)P2, PI(3,4)P2 et PI(3,4,5)P3, chez P. falciparum. Pour ce faire, nous avons exprimé chez le parasite des rapporteurs spécifiques correspondant à des domaines humains de liaison aux PI, fusionnés à une protéine fluorescente. Cette méthode nous a permis de confirmer des rapports antérieurs sur la localisation du PI3P dans la membrane de la vacuole alimentaire, dans de petites vésicules près ou sur la membrane plasmique du parasite ainsi qu’à l’apicoplaste. De plus, nous avons révélé pour la première fois la présence de PI5P chez P. falciparum et montré qu’il se localisait à la membrane plasmique, au noyau et potentiellement dans le réticulum endoplasmique de transition. Nous avons aussi montré que le PI4P est localisé dans la membrane plasmique ainsi que dans l’appareil de Golgi et que le PI(4,5)P2 est présent dans la membrane plasmique tout au long du cycle érythrocytaire. Cette carte de la distribution subcellulaire des PI constitue un excellent outil pour mieux déchiffrer les rôles de ces lipides chez le parasite P. falciparum. Dans le cadre d’un second projet, nous avons caractérisé une protéine possédant un domaine conservé chez les Apicomplexa, le domain d’homologie de la… Advisors/Committee Members: Richard, Dave.

Subjects/Keywords: Phospho-inositides; Plasmodium falciparum

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Ebrahimzadeh, Z. (2019). Exploring the roles of phosphoinositides in the biology of the malaria parasite Plasmodium falciparum. (Thesis). Université Laval. Retrieved from http://hdl.handle.net/20.500.11794/37075

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Ebrahimzadeh, Zeinab. “Exploring the roles of phosphoinositides in the biology of the malaria parasite Plasmodium falciparum.” 2019. Thesis, Université Laval. Accessed February 26, 2021. http://hdl.handle.net/20.500.11794/37075.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Ebrahimzadeh, Zeinab. “Exploring the roles of phosphoinositides in the biology of the malaria parasite Plasmodium falciparum.” 2019. Web. 26 Feb 2021.

Vancouver:

Ebrahimzadeh Z. Exploring the roles of phosphoinositides in the biology of the malaria parasite Plasmodium falciparum. [Internet] [Thesis]. Université Laval; 2019. [cited 2021 Feb 26]. Available from: http://hdl.handle.net/20.500.11794/37075.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Ebrahimzadeh Z. Exploring the roles of phosphoinositides in the biology of the malaria parasite Plasmodium falciparum. [Thesis]. Université Laval; 2019. Available from: http://hdl.handle.net/20.500.11794/37075

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Ghana

15. Mensah, B.A. Assessment of Genetic Diversity, Complexity of Infection of Plasmodium Falciparum and Resistance to Antimalarial Drugs in Two Ecological Zones in Ghana .

Degree: 2018, University of Ghana

URL: http://ugspace.ug.edu.gh/handle/123456789/32951

► Introduction: One major challenge to the global agenda for the elimination of malaria is the extensive genetic diversity of the parasite population, resulting in the… (more)

▼ Introduction: One major challenge to the global agenda for the elimination of malaria is the extensive genetic diversity of the parasite population, resulting in the development of drug resistance and variation in antigens targeted for vaccine development. The aim of this study was to decipher any ecological difference in the evolution of drug resistance and to determine how much the increased use of artemisinin-based combination therapies (ACTs) and other control interventions are shaping the genetic diversity of the Plasmodium falciparum population in two ecologically distinct populations in Ghana. Methods: A total of 803 dried blood spots (DBS) collected on filter paper from symptomatic children, aged 6 months to 14 years, with P. falciparum mono-infection in the coastal savanna (Cape-Coast) and the forest (Begoro) zones of Ghana from 2014 to 2017. In addition, a total of 991 P. falciparum infected DBS were collected from asymptomatic school children, aged 6 years to 14 years from 2013 to 2017. The study leveraged the high specificity and relatively low-cost of targeted next generation sequencing using molecular inversion probes for targeting and sequencing on the illumina MISEQ platform for sequencing of P. falciparum genes (pfcrt, pfdhfr, pfdhps, pfmdr1 and pfk13) implicated in anti-malarial resistance to chloroquine (CQ), sulfadoxine pyrimethamine (SP), lumefantrine, amodiaquine and artemisinin. The Plasmodium falciparum Apical Membrane Antigen 1 (pfama1) gene was also sequenced for the genetic diversity and complexity of P. falciparum infections (COI) analysis. Genetic diversity was compared between the two study populations and the sequences from Ghana were compared with sequences from West Africa, East Africa and South East Asia obtained from the gene bank. Results: The result showed high genetic diversity in pfama1 in Ghanaian sequences with a total of 164 pfama1 haplotypes and a haplotype diversity of 0.993. There was no genetic differentiation between the two study populations in Ghana. Parasite isolates from the two ecological zones in Ghana showed a moderate genetic differentiation with sequences from Thailand (Fst=0.054) and low differentiation with sequences from Kenya (Fst=0.004). Seventy three percent (73%) of the infections were monoclonal. The major molecular marker associated with CQ resistance pfcrt K76T significantly reduced over the four years of the study (χ² = 40.57; p<0.001). The rate of re-expansion of chloroquine sensitive strains pfcrt K76 was higher in the forest ecological zone compared to the coastal savanna zone. The pfmdr1 184F mutant associated with lumefantrine resistance remained high over the years (68% to 83%). The prevalence of the quadruple mutation (IRNGK), associated with sulfadoxine-pyrimethamine resistance is almost at fixation, whilst pfdhps 540E has remained very low in Ghana. In addition, the study found low prevalence of pfdhps 581G mutation associated with sulfadoxine resistance, which has not been previously reported in these parts of Ghana. The South East Asian…

Subjects/Keywords: Plasmodium Falciparum; Antimalarial Drugs; Ghana

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Mensah, B. A. (2018). Assessment of Genetic Diversity, Complexity of Infection of Plasmodium Falciparum and Resistance to Antimalarial Drugs in Two Ecological Zones in Ghana . (Doctoral Dissertation). University of Ghana. Retrieved from http://ugspace.ug.edu.gh/handle/123456789/32951

Chicago Manual of Style (16^th Edition):

Mensah, B A. “Assessment of Genetic Diversity, Complexity of Infection of Plasmodium Falciparum and Resistance to Antimalarial Drugs in Two Ecological Zones in Ghana .” 2018. Doctoral Dissertation, University of Ghana. Accessed February 26, 2021. http://ugspace.ug.edu.gh/handle/123456789/32951.

MLA Handbook (7^th Edition):

Mensah, B A. “Assessment of Genetic Diversity, Complexity of Infection of Plasmodium Falciparum and Resistance to Antimalarial Drugs in Two Ecological Zones in Ghana .” 2018. Web. 26 Feb 2021.

Vancouver:

Mensah BA. Assessment of Genetic Diversity, Complexity of Infection of Plasmodium Falciparum and Resistance to Antimalarial Drugs in Two Ecological Zones in Ghana . [Internet] [Doctoral dissertation]. University of Ghana; 2018. [cited 2021 Feb 26]. Available from: http://ugspace.ug.edu.gh/handle/123456789/32951.

Council of Science Editors:

Mensah BA. Assessment of Genetic Diversity, Complexity of Infection of Plasmodium Falciparum and Resistance to Antimalarial Drugs in Two Ecological Zones in Ghana . [Doctoral Dissertation]. University of Ghana; 2018. Available from: http://ugspace.ug.edu.gh/handle/123456789/32951

16. Dennis, Adelaide Sandra Mild. PfATP4 and the biochemical signature of PfATP4-associated compounds .

Degree: 2018, Australian National University

URL: http://hdl.handle.net/1885/149540

► In 2016 more than 200 million cases of malaria were reported and nearly 500 000 people died from the disease. Although there are antimalarial drugs… (more)

▼ In 2016 more than 200 million cases of malaria were reported and nearly 500 000 people died from the disease. Although there are antimalarial drugs available, the most virulent species of the disease-causing parasite, Plasmodium falciparum, has evolved some level of resistance to many of them. A global effort to accelerate antimalarial drug discovery has led to the finding that one particular parasite protein, the P-type ATPase PfATP4, appears to be the target of a number of potent novel antimalarial compounds. Two of these ‘PfATP4-associated compounds’ have entered the clinical pipeline; one of these is the spiroindolone KAE609 (also known as cipargamin). PfATP4 is located on the plasma membrane of the parasite. Although an early study yielded evidence consistent with PfATP4 being a Ca2+ transporter, more recent studies have provided evidence that PfATP4 extrudes Na+ ions from the parasite while importing H+ ions, allowing the parasite to maintain a large inward Na+ concentration gradient. When parasites are exposed to PfATP4-associated compounds, there is an increase in the Na+ concentration and the pH inside the parasite. In the work reported in this Thesis I have investigated the nature and function of PfATP4, extended the characterisation of the effects of PfATP4-associated compounds on mature asexual-stage P. falciparum parasites, and identified a number of new compounds of this class. A phylogenetic analysis revealed that PfATP4 belongs to a unique subgroup of Type II P-type ATPases, specific to apicomplexan parasites and their closest relatives. Previous studies have reported that PfATP4-associated compounds cause parasite swelling. The effect of KAE609 on the volume of isolated parasites and parasitised erythrocytes was characterised using a Coulter Multisizer. KAE609 caused isolated parasites to swell in a Na+-dependent manner. KAE609 also caused intact infected erythrocytes to swell and thereby increase in osmotic fragility. Another six PfATP4-associated compounds were also shown to induce parasite swelling. Protecting parasitised erythrocytes from excessive swelling by growing them in a hyperosmotic medium increased the concentration of KAE609 that was required to kill the parasite, consistent with cell swelling playing a role in the mechanism of action of KAE609. The ‘biochemical signature’ of PfATP4-associated compounds was used to identify novel PfATP4-associated compounds from a set of 400 diverse drug-like compounds. Experiments investigating the effects of these compounds on parasite Na+, pH and volume, as well as cross-resistance studies using KAE609-resistant parasites, provided evidence that eleven compounds act in a manner consistent with inhibition of PfATP4. These compounds add to the chemical diversity of known…

Subjects/Keywords: PfATP4; Plasmodium falciparum; malaria; spiroindolone

10 more images…

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APA (6^th Edition):

Dennis, A. S. M. (2018). PfATP4 and the biochemical signature of PfATP4-associated compounds . (Thesis). Australian National University. Retrieved from http://hdl.handle.net/1885/149540

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Dennis, Adelaide Sandra Mild. “PfATP4 and the biochemical signature of PfATP4-associated compounds .” 2018. Thesis, Australian National University. Accessed February 26, 2021. http://hdl.handle.net/1885/149540.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Dennis, Adelaide Sandra Mild. “PfATP4 and the biochemical signature of PfATP4-associated compounds .” 2018. Web. 26 Feb 2021.

Vancouver:

Dennis ASM. PfATP4 and the biochemical signature of PfATP4-associated compounds . [Internet] [Thesis]. Australian National University; 2018. [cited 2021 Feb 26]. Available from: http://hdl.handle.net/1885/149540.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Dennis ASM. PfATP4 and the biochemical signature of PfATP4-associated compounds . [Thesis]. Australian National University; 2018. Available from: http://hdl.handle.net/1885/149540

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Ghana

17. Puije, W.V.D. Interaction of Plasmodium Falciparum Antigens with Blood Group Determinants: Preferences and Link to Disease Severity .

Degree: 2019, University of Ghana

URL: http://ugspace.ug.edu.gh/handle/123456789/35796

► The blood type of malaria patients may determine the outcome of disease. Blood group O associates with protection, while non-O blood groups (A, B or… (more)

▼ The blood type of malaria patients may determine the outcome of disease. Blood group O associates with protection, while non-O blood groups (A, B or AB) are associated with severe malaria. Rosetting of red blood cells (RBC), which is mediated by blood group A and other red blood cell surface molecules, is also associated with severe disease in the two most virulent species of Plasmodium that infect humans. The interaction of parasite ligands with blood group carbohydrates on RBC and endothelial receptors leads to infected RBC sequestration and persistence of the parasite in the vasculature. The objectives of this work were to study and measure the interactions between parasite-expressed factors on the red cell surface and blood group molecules using standard and novel methods, in both laboratory strains and field isolates of Plasmodium falciparum. Expression levels of var and rif transcripts in laboratory isolates selected for binding to blood group antigens were determined, while antibody reactivity to surface antigens were investigated in these isolates. Venous blood samples were collected from 113 children aged 12 years and below diagnosed with malaria and resident in Hohoe, a high malaria transmission zone in the Volta Region of Ghana. The blood type was determined for all patients, while plasma and RBCs were processed and stored for subsequent work. Long term blood group A and B binding variants of laboratory strains of P. falciparum 3D7, FMG and FUP parasites were successfully produced by regular panning on immobilized blood group oligosaccharides, though the production of binding variants in other strains (FCR3 and HB3) were not as successful. Selected parasites were found to bind to both A and B blood group carbohydrates irrespective of the blood group antigen used in selection. The binding isolates also showed a marked adhesion to aorta and dermal endothelial cell (EC) lines. A novel microtiter-plate based quantitative assay to specifically measure binding of parasite infected RBC to plate-bound blood group antigens was developed and used to determine the interaction of both laboratory and field strains to the blood group A and B antigens. All blood group antigen-selected isolates showed a strong propensity to form rosettes in the presence of RBC from donors of blood type A, AB or B, in contrast to unselected isogenic isolates which did not form rosettes under the same conditions. The transcription levels of 58 var genes of ring stage parasites of 3D7 and FMG selected on blood group antigens and detected by quantitative real-time polymerase chain reaction (QPCR) showed no consistent pattern of expression between selected and unselected parasites, though the adhesion linked genes Pf13_0003 and IT4var32b of 3D7 and FMG, respectively, had elevated levels in some selected isolates. In conclusion, a novel plate-based assay to directly measure infected erythrocyte adhesion to blood group antigens has been developed. Selection for long term blood group specific parasite binding isolates was also achieved, but…

Subjects/Keywords: Plasmodium Falciparum; Antigens; Blood Group

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Puije, W. V. D. (2019). Interaction of Plasmodium Falciparum Antigens with Blood Group Determinants: Preferences and Link to Disease Severity . (Doctoral Dissertation). University of Ghana. Retrieved from http://ugspace.ug.edu.gh/handle/123456789/35796

Chicago Manual of Style (16^th Edition):

Puije, W V D. “Interaction of Plasmodium Falciparum Antigens with Blood Group Determinants: Preferences and Link to Disease Severity .” 2019. Doctoral Dissertation, University of Ghana. Accessed February 26, 2021. http://ugspace.ug.edu.gh/handle/123456789/35796.

MLA Handbook (7^th Edition):

Puije, W V D. “Interaction of Plasmodium Falciparum Antigens with Blood Group Determinants: Preferences and Link to Disease Severity .” 2019. Web. 26 Feb 2021.

Vancouver:

Puije WVD. Interaction of Plasmodium Falciparum Antigens with Blood Group Determinants: Preferences and Link to Disease Severity . [Internet] [Doctoral dissertation]. University of Ghana; 2019. [cited 2021 Feb 26]. Available from: http://ugspace.ug.edu.gh/handle/123456789/35796.

Council of Science Editors:

Puije WVD. Interaction of Plasmodium Falciparum Antigens with Blood Group Determinants: Preferences and Link to Disease Severity . [Doctoral Dissertation]. University of Ghana; 2019. Available from: http://ugspace.ug.edu.gh/handle/123456789/35796

University of Ghana

18. Bortier, E. Prevalence of PFHRP2 and/or PFHRP3 Gene Deletions in Plasmodium Falciparum Isolates and the Performance of HRP2 Based Malaria Rapid Diagnostic Tests .

Degree: 2019, University of Ghana

URL: http://ugspace.ug.edu.gh/handle/123456789/35823

► BACKGROUND: Malaria rapid diagnostic tests (MRDTs) are important for malaria disease management. However, performance of the RDTs is affected when the targeted antigens in the… (more)

▼ BACKGROUND: Malaria rapid diagnostic tests (MRDTs) are important for malaria disease management. However, performance of the RDTs is affected when the targeted antigens in the parasite have a variation or are altogether absent. The most common parasite target antigen in RDTs, Plasmodium falciparum histidine-rich protein 2 (HRP2), has been reported to be absent in some P. falciparum parasites. Loss of the pfhrp2 in P. falciparum parasites affects the accuracy of PfHRP2 based RDT kits when they are used in malaria diagnosis. Thus, to control malaria, determining where and how often P. falciparum parasites not having pfhrp2 occur, is very important. AIM: The aim was to investigate the prevalence of pfhrp2 and/or pfhrp3 gene deletions in P. falciparum isolates from southern Ghana and the performance of the currently used PfHRP2 based MRDTs. METHODS: Samples were collected from sites in the southern part of Ghana: three Cocoa Clinics (Accra, Tafo, and Kumasi) and from three other health facilities, Ussher and Mamprobi polyclinics and 37 Military Hospital, all in Accra. Patients with febrile illness, referred by a clinician to the laboratories of these health facilities for a malaria test, were recruited. Blood samples for thick and thin blood smears, for malaria microscopy, and filter paper blots were obtained. All blood samples were tested using a HRP2-based malaria RDT. DNA was extracted from the dried filter paper blood blots using the TNES (Tris HCl, EDTA, NaCl, and SDS) buffer protocol. Plasmodium falciparum infection was confirmed by polymerase chain reaction (PCR). The presence of pfhrp2 and pfhrp3 genes was investigated by PCR. RESULTS: A total of 371 patient samples, from Accra (58.5%), Kumasi (21.3%) and from Tafo (20.2%), were used in the study. PCR provided the highest number, 14.8% (55/371), of positive detections for falciparum infections. Microscopy detected parasites in 20/261 (7.7%) samples and the minimum parasite density by microscopy was 430 parasites/μL. Out of the 371 samples, 27 (7.3%) were positive by RDT. The highest RDT positivity rate, 13.3% (10/75), was observed at Tafo. False negative RDT results were obtained in 43/55 (78.2%) of the negative branded RDT kits. Only two microscopy positive sample were RDT positive. Using 18SrDNA PCR, 55 (14.8%) samples were positive for P. falciparum. In Accra, 79.2 % (19/24) of the PCR positive samples had P. falciparum parasites that lacked exon 2 of pfhrp2. In Tafo, on the other hand, only 7.4% (2/27) of the PCR positive samples had P. falciparum parasites that lacked exon 2 of pfhrp2. None of PCR positive samples had P. falciparum parasites that lacked exon 2 of pfhrp2 in Kumasi. Only 33.3% (8/24) samples, all from Accra, lacked exon 2 of pfhrp3. In total, 38.1% (8/21) of the samples contained parasites that lacked exon 2 of both pfhrp2 and pfhrp3. Fourteen negative- branded PfHRP2 RDT isolates, consisting of 13 (92.9%) samples from Accra and 1 (7.1%) from Tafo, were negative for the pfhrp2 gene (pfhrp2- ). Two samples,…

Subjects/Keywords: PFHRP2; Malaria; PFHRP3; Plasmodium Falciparum

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Bortier, E. (2019). Prevalence of PFHRP2 and/or PFHRP3 Gene Deletions in Plasmodium Falciparum Isolates and the Performance of HRP2 Based Malaria Rapid Diagnostic Tests . (Masters Thesis). University of Ghana. Retrieved from http://ugspace.ug.edu.gh/handle/123456789/35823

Chicago Manual of Style (16^th Edition):

Bortier, E. “Prevalence of PFHRP2 and/or PFHRP3 Gene Deletions in Plasmodium Falciparum Isolates and the Performance of HRP2 Based Malaria Rapid Diagnostic Tests .” 2019. Masters Thesis, University of Ghana. Accessed February 26, 2021. http://ugspace.ug.edu.gh/handle/123456789/35823.

MLA Handbook (7^th Edition):

Bortier, E. “Prevalence of PFHRP2 and/or PFHRP3 Gene Deletions in Plasmodium Falciparum Isolates and the Performance of HRP2 Based Malaria Rapid Diagnostic Tests .” 2019. Web. 26 Feb 2021.

Vancouver:

Bortier E. Prevalence of PFHRP2 and/or PFHRP3 Gene Deletions in Plasmodium Falciparum Isolates and the Performance of HRP2 Based Malaria Rapid Diagnostic Tests . [Internet] [Masters thesis]. University of Ghana; 2019. [cited 2021 Feb 26]. Available from: http://ugspace.ug.edu.gh/handle/123456789/35823.

Council of Science Editors:

Bortier E. Prevalence of PFHRP2 and/or PFHRP3 Gene Deletions in Plasmodium Falciparum Isolates and the Performance of HRP2 Based Malaria Rapid Diagnostic Tests . [Masters Thesis]. University of Ghana; 2019. Available from: http://ugspace.ug.edu.gh/handle/123456789/35823

University of Melbourne

19. Boyle, Michelle Jacqueline. Plasmodium falciparum merozoite invasion mechanisms and inhibitors of invasion.

Degree: 2012, University of Melbourne

URL: http://hdl.handle.net/11343/37321

► Malaria threatens 40% of the global population resulting in approximately 225 million cases of disease and 800 000 deaths per year. Recently marked improvements in… (more)

▼ Malaria threatens 40% of the global population resulting in approximately 225 million cases of disease and 800 000 deaths per year. Recently marked improvements in the implementation of control measures and increased use of artemisinin combination therapies (ACTs) have contributed to a reduction in malaria mortality and morbidity (World Health Organization, Global Malaria Programme, 2010). Furthermore the licensing and roll-out of the first malaria vaccine, RTS,S, is hoped to occur by 2015 (White, 2011; Agnandji et al., 2011). However, a sustained reduction in malaria burden and eradication of malaria seems unlikely with current control strategies alone. The largest malaria burden is caused by infection with P. falciparum parasites. All symptomatic illness occurs during asexual replication in the blood that is initiated when the merozoite form of the parasite invades red blood cells (RBCs). A limited understanding of merozoite invasion and immune mechanisms inhibiting invasion has hampered rational vaccine and drug development targeting this stage of the parasite life cycle. This thesis is based on the study of merozoite invasion of the RBC with a particular focus on the role of merozoite surface proteins in invasion and mechanisms of inhibition targeting these proteins. Part of the difficulty in studying P. falciparum merozoite invasion is due to the lack of efficient techniques to isolate merozoites that maintain invasive capacity. Chapter 3 describes the development of methods to isolate merozoites that maintain viability. Importantly, the method only requires basic laboratory equipment, therefore is accessible to both resource rich and developing laboratories. Highly synchronized cultures were treated with a cysteine protease inhibitor to block schizont rupture and then merozoites isolated via membrane filtration (Boyle et al., 2010a). Approximately 15% of isolated merozoites maintained viability and were able to successfully invade when incubated with RBCs. This allowed for the development of methods to fix merozoites during invasion for microscopy and invasion inhibition assays. Invasion of isolated merozoites was independent of serum components and the invasive half-life of merozoites was approximately 8 minutes. Merozoite isolation and invasion assays are now being used by a number of research groups and are a powerful technique to study merozoite invasion mechanisms (Riglar et al., 2011) and inhibitors of invasion. In Chapter 4, microscopy of invading merozoites is used to investigate the shedding of merozoite surface antigens during invasion. The initial steps of merozoite invasion are hypothesized to be mediated by merozoite surface proteins that contact with the RBC via weak receptor-ligand interactions. During invasion it is thought that merozoite surface proteins are cleaved and then shed from the merozoite to allow invasion to occur. This has been most clearly demonstrated for MSP1, with compounds that inhibit…

Subjects/Keywords: Plasmodium falciparum; merozoite; invasion; inhibitors

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Boyle, M. J. (2012). Plasmodium falciparum merozoite invasion mechanisms and inhibitors of invasion. (Doctoral Dissertation). University of Melbourne. Retrieved from http://hdl.handle.net/11343/37321

Chicago Manual of Style (16^th Edition):

Boyle, Michelle Jacqueline. “Plasmodium falciparum merozoite invasion mechanisms and inhibitors of invasion.” 2012. Doctoral Dissertation, University of Melbourne. Accessed February 26, 2021. http://hdl.handle.net/11343/37321.

MLA Handbook (7^th Edition):

Boyle, Michelle Jacqueline. “Plasmodium falciparum merozoite invasion mechanisms and inhibitors of invasion.” 2012. Web. 26 Feb 2021.

Vancouver:

Boyle MJ. Plasmodium falciparum merozoite invasion mechanisms and inhibitors of invasion. [Internet] [Doctoral dissertation]. University of Melbourne; 2012. [cited 2021 Feb 26]. Available from: http://hdl.handle.net/11343/37321.

Council of Science Editors:

Boyle MJ. Plasmodium falciparum merozoite invasion mechanisms and inhibitors of invasion. [Doctoral Dissertation]. University of Melbourne; 2012. Available from: http://hdl.handle.net/11343/37321

20. Santolalla Robles, Meddly Leslye. Prevalencia de mutaciones en los genes PFDHFR y PFDHPS de Plasmodium falciparum en muestras de pacientes con malaria severa y/o complicada, del banco de muestras biológicas del NAMRU-6.

Degree: 2015, National University of San Marcos

URL: http://hdl.handle.net/20.500.12672/4620

► – Introduction: Malaria represents a medical emergency because it may rapidly progress to complication and death without prompt and appropriate treatment. Severe and/or complicated malaria… (more)

Subjects/Keywords: Mutación; Plasmodium falciparum; Malaria

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APA (6^th Edition):

Santolalla Robles, M. L. (2015). Prevalencia de mutaciones en los genes PFDHFR y PFDHPS de Plasmodium falciparum en muestras de pacientes con malaria severa y/o complicada, del banco de muestras biológicas del NAMRU-6. (Thesis). National University of San Marcos. Retrieved from http://hdl.handle.net/20.500.12672/4620

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Santolalla Robles, Meddly Leslye. “Prevalencia de mutaciones en los genes PFDHFR y PFDHPS de Plasmodium falciparum en muestras de pacientes con malaria severa y/o complicada, del banco de muestras biológicas del NAMRU-6.” 2015. Thesis, National University of San Marcos. Accessed February 26, 2021. http://hdl.handle.net/20.500.12672/4620.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Santolalla Robles, Meddly Leslye. “Prevalencia de mutaciones en los genes PFDHFR y PFDHPS de Plasmodium falciparum en muestras de pacientes con malaria severa y/o complicada, del banco de muestras biológicas del NAMRU-6.” 2015. Web. 26 Feb 2021.

Vancouver:

Santolalla Robles ML. Prevalencia de mutaciones en los genes PFDHFR y PFDHPS de Plasmodium falciparum en muestras de pacientes con malaria severa y/o complicada, del banco de muestras biológicas del NAMRU-6. [Internet] [Thesis]. National University of San Marcos; 2015. [cited 2021 Feb 26]. Available from: http://hdl.handle.net/20.500.12672/4620.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Santolalla Robles ML. Prevalencia de mutaciones en los genes PFDHFR y PFDHPS de Plasmodium falciparum en muestras de pacientes con malaria severa y/o complicada, del banco de muestras biológicas del NAMRU-6. [Thesis]. National University of San Marcos; 2015. Available from: http://hdl.handle.net/20.500.12672/4620

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Addis Ababa University

21. Ahmed, Muzemil. Determination of Artemisinin and essential oil contents of Artemisia annua L. grown in Ethiopia and In vivo Antimalarial activity of its crude extracts against Plasmodium berghei in mice .

Degree: 2009, Addis Ababa University

URL: http://etd.aau.edu.et/dspace/handle/123456789/3662

► Abstract Malaria is a major public health problem in the world in general and developing country in particular. It causes about 1.5-3 millions deaths per… (more)

▼ Abstract Malaria is a major public health problem in the world in general and developing country in particular. It causes about 1.5-3 millions deaths per year, an annual incidence of 300-500 million clinical cases. Chloroquine has been the drug of choice for chemoprophylaxis and treatment of P. falciparum for several decades, but its clinical utility greatly reduced in many malaria endemic regions due to the spread of chloroquine resistance. Chinese investigators introduced a new compound, artemisinin, a sesquiterpene lactone, from Artemisia annua a few years back. It possesses a potent antimalarial activity with different mechanism of action from that of the conventional drugs in use. Currently the plant is introduced to many African countries including Ethiopia. Reports at different countries showed the variability of artemisinin content for the same plant grown at different geographical regions. Seventy percent ethanol and hot water crude extracts from the dried leaves of A. annua grown at Wondogenet (Ethiopia) gave 20.2 and 14.00 % yields per dry weight respectively. Preliminary phytochemical screening using standard procedures showed the presence of steroidal compounds, phenolics, flavonoids and trace amount of tannins. Quantification of artemisinin from leaf of crude ethanol extract of A. annua was carried out using HPLC-UV with an attempt to show the plant is biosynthesizing artemisinin. The limiting step in sample preparation was the presence of large amounts of chlorophyll in the crude extract. The standard calibration curve for the quantification was linear with correlation coefficient of 0.96150. In this study the average content of artemisinin (w/w) was determined to be low in concentration (0.014% ± 0.001). Analysis of essential oil (0.32%) from A. annua by GC/MS gave a total of 38 compounds comprised of 99.72 % of the total volatile constituents and of these twenty-two monoterpenes (57.89%), fourteen sesquiterpens (36.84%) and two phenols (5.55%) were identified. Among the volatile constituents camphor was identified to be the major component (43.84%). An in vivo experiment was performed to know weather A. annua grown in Ethiopia has retained its antimalarial activity. The two crude extracts at three different doses viii were tested on mice infected with Plasmodium berghei, in a four day test procedure. The results of the in vivo experiment showed that the parasite multiplication was inhibited by 77.97 % and 64.75 % for 70 % ethanol and hot water extracts respectively at dose level of 450 mg/kg. The study revealed that A. annua grown in Ethiopia retained the desired antiplasmodial activity, as seen from its suppressive effects against P. berghei. Advisors/Committee Members: Dr. Ariaya Hymete,Dr. Mekuria Tadesse,Ato Abebe Animute (advisor).

Subjects/Keywords: Malaria; Plasmodium falciparum; Plasmodium berghei; Artemisia annua

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APA (6^th Edition):

Ahmed, M. (2009). Determination of Artemisinin and essential oil contents of Artemisia annua L. grown in Ethiopia and In vivo Antimalarial activity of its crude extracts against Plasmodium berghei in mice . (Thesis). Addis Ababa University. Retrieved from http://etd.aau.edu.et/dspace/handle/123456789/3662

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Ahmed, Muzemil. “Determination of Artemisinin and essential oil contents of Artemisia annua L. grown in Ethiopia and In vivo Antimalarial activity of its crude extracts against Plasmodium berghei in mice .” 2009. Thesis, Addis Ababa University. Accessed February 26, 2021. http://etd.aau.edu.et/dspace/handle/123456789/3662.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Ahmed, Muzemil. “Determination of Artemisinin and essential oil contents of Artemisia annua L. grown in Ethiopia and In vivo Antimalarial activity of its crude extracts against Plasmodium berghei in mice .” 2009. Web. 26 Feb 2021.

Vancouver:

Ahmed M. Determination of Artemisinin and essential oil contents of Artemisia annua L. grown in Ethiopia and In vivo Antimalarial activity of its crude extracts against Plasmodium berghei in mice . [Internet] [Thesis]. Addis Ababa University; 2009. [cited 2021 Feb 26]. Available from: http://etd.aau.edu.et/dspace/handle/123456789/3662.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Ahmed M. Determination of Artemisinin and essential oil contents of Artemisia annua L. grown in Ethiopia and In vivo Antimalarial activity of its crude extracts against Plasmodium berghei in mice . [Thesis]. Addis Ababa University; 2009. Available from: http://etd.aau.edu.et/dspace/handle/123456789/3662

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

22. Sethia, Sonal. Etudes métabolomiques du métabolisme du carbone des stades érythrocytaires asexués du parasite du paludisme humain Plasmodium falciparum. : Use of metabolomics to decipher parasite carbon metabolism of asexual erythrocytic stages of the human malaria parasite Plasmodium falciparum.

Degree: Docteur es, Biologie Santé, 2015, Montpellier

URL: http://www.theses.fr/2015MONTT046

► Le paludisme est une des maladies tropicales les plus dévastatrices au monde causée par des parasites protozoaires intracellulaires du genre Plasmodium. Cinq espèces de plasmodies… (more)

▼ Le paludisme est une des maladies tropicales les plus dévastatrices au monde causée par des parasites protozoaires intracellulaires du genre Plasmodium. Cinq espèces de plasmodies sont responsables du paludisme chez l'homme et causent 600 000 décès par an principalement chez les enfants de moins de 5 ans et les femmes enceintes vivant dans les régions les plus pauvres du globe. Les parasites ont généré une résistance contre les chimiothérapies existantes et aucun vaccin efficace n'est encore disponible. Il est donc impératif d'identifier et de valider de nouvelles cibles qui peuvent être exploitées pour la découverte de nouveaux médicaments.Cette étude a porté sur la caractérisation d'un enzyme, la phosphoénolpyruvate carboxylase (PEPC), produit d'un gène spécifique au parasite et absent chez l'hôte humain, ce qui constitue l'un des pré-requis d'une cible potentielle pour la découverte de médicaments. Le gène avait été montré comme essentiel pour des parasites seulement en absence de malate ou de fumarate, suggérant un rôle de la protéine dans le métabolisme du carbone intermédiaire des parasites.Mes études de thèse avaient pour but de caractériser le rôle de la PEPC en utilisant la métabolomique. J'ai d'abord établi et normalisé une méthodologie d'analyses métabolomiques des globules rouges infectés par Plasmodium et optimisé l'analyse des métabolites hydrophiles présents dans le parasite intracellulaire et sa cellule hôte. Nous nous sommes concentrés sur les métabolites du métabolisme du carbone intermédiaire, où la PEPC pouvait jouer un rôle déterminant par analogie avec les plantes et les bactéries. Des analyses ciblées utilisant un marquage isotopique du métabolome à partir de 13C-U-glucose, 13C-bicarbonate et 13C, 15N-glutamine ont aussi été réalisées permettant de mieux appréhender les conséquences d'un KO de l'enzyme PEPC sur le métabolisme du parasite.Les données montrent que l'enzyme PEPC permet une fixation du bicarbonate et catalyse une réaction anaplérotique conduisant à du malate qui est introduit dans le cycle de l'acide tricarboxylique mitochondrial, transférant ainsi des équivalents réducteurs du cytoplasme à la mitochondrie et fournissant aussi un point d'entrée du squelette carboné dans le cycle. Les résultats montrent surtout que les parasites possèdent un cycle complet et de type oxydatif de l'acide tricarboxylique mitochondrial. Il parait y avoir trois points d'entrée: 1. l'acétyl CoA résultant du pyruvate généré par la glycolyse et décarboxylé dans la mitochondrie; 2. l'acide alpha-cétoglutarique provenant du glutamate, qui lui-même résulte de la désamination de la glutamine essentiellement fournie par l'environnement externe; 3. le malate, produit en aval de la malate déshydrogénase qui réduit l'oxaloacétate produit par la PEPC. En aval de la PEPC, la biosynthèse des pyrimidines opère grâce à l'activité de l'aspartate aminotransférase agissant sur oxaloacétate.En dehors du malate, le fumarate est le seul autre métabolite qui permet de s'opposer au défaut de croissance des parasites déficients… Advisors/Committee Members: Müller, Sylke (thesis director), Vial, Henri (thesis director).

Subjects/Keywords: Plasmodium Falciparum; Métabolomique; Métabolisme du carbone; Plasmodium Falciparum; Metabolomics; Carbon metabolism

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Sethia, S. (2015). Etudes métabolomiques du métabolisme du carbone des stades érythrocytaires asexués du parasite du paludisme humain Plasmodium falciparum. : Use of metabolomics to decipher parasite carbon metabolism of asexual erythrocytic stages of the human malaria parasite Plasmodium falciparum. (Doctoral Dissertation). Montpellier. Retrieved from http://www.theses.fr/2015MONTT046

Chicago Manual of Style (16^th Edition):

Sethia, Sonal. “Etudes métabolomiques du métabolisme du carbone des stades érythrocytaires asexués du parasite du paludisme humain Plasmodium falciparum. : Use of metabolomics to decipher parasite carbon metabolism of asexual erythrocytic stages of the human malaria parasite Plasmodium falciparum.” 2015. Doctoral Dissertation, Montpellier. Accessed February 26, 2021. http://www.theses.fr/2015MONTT046.

MLA Handbook (7^th Edition):

Sethia, Sonal. “Etudes métabolomiques du métabolisme du carbone des stades érythrocytaires asexués du parasite du paludisme humain Plasmodium falciparum. : Use of metabolomics to decipher parasite carbon metabolism of asexual erythrocytic stages of the human malaria parasite Plasmodium falciparum.” 2015. Web. 26 Feb 2021.

Vancouver:

Sethia S. Etudes métabolomiques du métabolisme du carbone des stades érythrocytaires asexués du parasite du paludisme humain Plasmodium falciparum. : Use of metabolomics to decipher parasite carbon metabolism of asexual erythrocytic stages of the human malaria parasite Plasmodium falciparum. [Internet] [Doctoral dissertation]. Montpellier; 2015. [cited 2021 Feb 26]. Available from: http://www.theses.fr/2015MONTT046.

Council of Science Editors:

Sethia S. Etudes métabolomiques du métabolisme du carbone des stades érythrocytaires asexués du parasite du paludisme humain Plasmodium falciparum. : Use of metabolomics to decipher parasite carbon metabolism of asexual erythrocytic stages of the human malaria parasite Plasmodium falciparum. [Doctoral Dissertation]. Montpellier; 2015. Available from: http://www.theses.fr/2015MONTT046

Université Montpellier II

23. Pulcini, Serena. Studies on the mechanisms of action of artemisinins and the role of PfATP6 : Les études sur les mécanismes d'action de l'artémisinine et le rôle des PfATP6.

Degree: Docteur es, Interface chimie-Biologie : systèmes moléculaires à visée thérapeutique, 2011, Université Montpellier II

URL: http://www.theses.fr/2011MON20138

► La pompe ATPase Ca2+ du réticulum sarco-endoplasmique Plasmodium falciparum (PfATP6) est une protéine de dix transmembranes, impliqué dans la régulation de l'homéostasie du calcium dans… (more)

▼ La pompe ATPase Ca2+ du réticulum sarco-endoplasmique Plasmodium falciparum (PfATP6) est une protéine de dix transmembranes, impliqué dans la régulation de l'homéostasie du calcium dans le parasite. L'importance d'étudier cette protéine repose sur l'hypothèse d'être engagé dans le mécanisme d'action et de résistance des artémisinines. Des travaux précédents, fondé sur l'expression hétérologue dans des ovocytes de Xenopus laevis et Saccharomyces cerevisiae, ont montré des résultats opposés, générant de nombreux corollaires vérifiables. Par conséquent, des travaux supplémentaires sont nécessaires pour mieux comprendre la nature des interactions entre les artémisinines et transporteurs de type SERCA.Afin d'évaluer le caractère essentiel du gène de Plasmodium spp., une approche de génétique inverse a été utilisée. Knockout du gène, soit P. falciparum et berghei, ne pouvant pas être obtenu. La complémentation de sauvetage épisomique a été jugée impossible. Marquage à la fin 3' de PfATP6 et PbATP6 a été, également, tenté pour étudier la localisation et l'expression de la protéine chez les parasites. La manipulation des gènes à cette place n'a pas permis la survie du parasite. Nos résultats, pris ensemble, montrent que ATP6 est essentiel dans Plasmodium spp..Au cours de nos études génétiques, un phénotype stable et particulier de parasites du genre Plasmodium falciparum 3D7 a été distingué. Les étranges parasites “monstres" contiennent une vacuole digestive inhabituelle gonflées à travers toutes les étapes du développement du parasite. Caractérisation de l'insolite Plasmodium a été réalisée, montrant une sensibilité accrue à la chloroquine, mais pas à l'artémisinine ou de la méfloquine. Tenant compte de la similitude du PfATP6 avec la pompe SERCA orthologue mammifère, de nouvelles molécules, connu et synthétisé pour cibler spécifiquement la protéine chez les mammifères, ont été testés sur P. falciparum. Quatre classes différentes de composés (sHA 14-1, BHQ, chalcone et des analogues de l'ACP) a montré le blocage de la croissance in vitro du P. falciparum 3D7 et Dd2 à des concentrations inférieure au range micromolaire. En outre, une nouvelle classe de molécules (thaperoxides), conçu comme un hybride entre l'artémisinine et thapsigargine, a été testé contre le type sauvage 7G8 et la ligne muté L263E. Ce dernier porte une mutation ponctuelle unique de nucléotides dans PfATP6, déjà connu d'être impliqué dans la résistance du l'artémisinine.Compte tenu de la difficulté à manipuler les gènes du parasite, et afin de mieux caractériser PfATP6, un gène synthétique a été optimisé pour l'expression hétérologue chez S. cerevisiae. De cette façon, la complémentation d'une ligne de levure mutée (K616) sans les pompes endogènes Ca2+ de type P a été permis avec succès, montrant le sauvetage de la croissance de la levure en présence de forte concentration de calcium libre. Différents inhibiteurs de SERCA, comme la thapsigargine et l'acide cyclopiazonique, ont été testés sur la levure complémenté K616 PfATP6, afin de vérifier l'inhibition… Advisors/Committee Members: Krishna, Sanjeev (thesis director).

Subjects/Keywords: Paludisme; Plasmodium falciparum; Artémisinines; PfATP6; Malaria; Plasmodium falciparum; Artemisinins; PfATP6

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Pulcini, S. (2011). Studies on the mechanisms of action of artemisinins and the role of PfATP6 : Les études sur les mécanismes d'action de l'artémisinine et le rôle des PfATP6. (Doctoral Dissertation). Université Montpellier II. Retrieved from http://www.theses.fr/2011MON20138

Chicago Manual of Style (16^th Edition):

Pulcini, Serena. “Studies on the mechanisms of action of artemisinins and the role of PfATP6 : Les études sur les mécanismes d'action de l'artémisinine et le rôle des PfATP6.” 2011. Doctoral Dissertation, Université Montpellier II. Accessed February 26, 2021. http://www.theses.fr/2011MON20138.

MLA Handbook (7^th Edition):

Pulcini, Serena. “Studies on the mechanisms of action of artemisinins and the role of PfATP6 : Les études sur les mécanismes d'action de l'artémisinine et le rôle des PfATP6.” 2011. Web. 26 Feb 2021.

Vancouver:

Pulcini S. Studies on the mechanisms of action of artemisinins and the role of PfATP6 : Les études sur les mécanismes d'action de l'artémisinine et le rôle des PfATP6. [Internet] [Doctoral dissertation]. Université Montpellier II; 2011. [cited 2021 Feb 26]. Available from: http://www.theses.fr/2011MON20138.

Council of Science Editors:

Pulcini S. Studies on the mechanisms of action of artemisinins and the role of PfATP6 : Les études sur les mécanismes d'action de l'artémisinine et le rôle des PfATP6. [Doctoral Dissertation]. Université Montpellier II; 2011. Available from: http://www.theses.fr/2011MON20138

University of Ghana

24. Quansah, E.B. Functional Characterization of Plasmodium Falciparum Claudin-Like Apicomplexan Microneme Protein (Pfclamp) (Pf3d7_1030200) .

Degree: 2019, University of Ghana

URL: http://ugspace.ug.edu.gh/handle/123456789/34631

► Malaria is still a public health burden. With the recent reports of artemisinin resistance in Plasmodium falciparum coupled with the low efficacy of the RTS,… (more)

Subjects/Keywords: Malaria; Plasmodium Falciparum; Ligand-Receptor; Erythrocyte Invasion; Plasmodium Falciparum

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Quansah, E. B. (2019). Functional Characterization of Plasmodium Falciparum Claudin-Like Apicomplexan Microneme Protein (Pfclamp) (Pf3d7_1030200) . (Masters Thesis). University of Ghana. Retrieved from http://ugspace.ug.edu.gh/handle/123456789/34631

Chicago Manual of Style (16^th Edition):

Quansah, E B. “Functional Characterization of Plasmodium Falciparum Claudin-Like Apicomplexan Microneme Protein (Pfclamp) (Pf3d7_1030200) .” 2019. Masters Thesis, University of Ghana. Accessed February 26, 2021. http://ugspace.ug.edu.gh/handle/123456789/34631.

MLA Handbook (7^th Edition):

Quansah, E B. “Functional Characterization of Plasmodium Falciparum Claudin-Like Apicomplexan Microneme Protein (Pfclamp) (Pf3d7_1030200) .” 2019. Web. 26 Feb 2021.

Vancouver:

Quansah EB. Functional Characterization of Plasmodium Falciparum Claudin-Like Apicomplexan Microneme Protein (Pfclamp) (Pf3d7_1030200) . [Internet] [Masters thesis]. University of Ghana; 2019. [cited 2021 Feb 26]. Available from: http://ugspace.ug.edu.gh/handle/123456789/34631.

Council of Science Editors:

Quansah EB. Functional Characterization of Plasmodium Falciparum Claudin-Like Apicomplexan Microneme Protein (Pfclamp) (Pf3d7_1030200) . [Masters Thesis]. University of Ghana; 2019. Available from: http://ugspace.ug.edu.gh/handle/123456789/34631

Universidad del Rosario

25. Patiño Molano, Liliana Catherine. Caracterización de la proteína del cuello de las roptrias 5 en plasmodium falciparum (PfRON5).

Degree: 2011, Universidad del Rosario

URL: http://repository.urosario.edu.co/handle/10336/2678

►

El conocimiento de las proteínas implicadas en el proceso de invasión de los merozoitos a los eritrocitos por Plasmodium es el punto de partida para… (more)

Subjects/Keywords: Malaria; Homología; Merozoito; Plasmodium falciparum; MALARIA - INVESTIGACIONES; EPIDEMIOLOGIA - INVESTIGACIONES; Malaria; Homology; Merozoite; Plasmodium falciparum; PLASMODIUM FALCIPARUM - INVESTIGACIONES

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Patiño Molano, L. C. (2011). Caracterización de la proteína del cuello de las roptrias 5 en plasmodium falciparum (PfRON5). (Thesis). Universidad del Rosario. Retrieved from http://repository.urosario.edu.co/handle/10336/2678

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Patiño Molano, Liliana Catherine. “Caracterización de la proteína del cuello de las roptrias 5 en plasmodium falciparum (PfRON5).” 2011. Thesis, Universidad del Rosario. Accessed February 26, 2021. http://repository.urosario.edu.co/handle/10336/2678.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Patiño Molano, Liliana Catherine. “Caracterización de la proteína del cuello de las roptrias 5 en plasmodium falciparum (PfRON5).” 2011. Web. 26 Feb 2021.

Vancouver:

Patiño Molano LC. Caracterización de la proteína del cuello de las roptrias 5 en plasmodium falciparum (PfRON5). [Internet] [Thesis]. Universidad del Rosario; 2011. [cited 2021 Feb 26]. Available from: http://repository.urosario.edu.co/handle/10336/2678.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Patiño Molano LC. Caracterización de la proteína del cuello de las roptrias 5 en plasmodium falciparum (PfRON5). [Thesis]. Universidad del Rosario; 2011. Available from: http://repository.urosario.edu.co/handle/10336/2678

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Rhodes University

26. Goble, Jessica Leigh. The druggable antimalarial target 1-deoxy-D-xylulose-5-phosphate reductoisomerase: purfication, kinetic characterization and inhibition studies.

Degree: Faculty of Science, Biochemistry, Microbiology and Biotechnology, 2011, Rhodes University

URL: http://hdl.handle.net/10962/d1004008

► Plasmodium falciparum 1–deoxy–D–xylulose–5 phosphatereductoisomerase (PfDXR) plays a role in isoprenoid biosynthesis in the malaria parasite and is absent in the human host, making this parasite… (more)

▼ Plasmodium falciparum 1–deoxy–D–xylulose–5 phosphatereductoisomerase (PfDXR) plays a role in isoprenoid biosynthesis in the malaria parasite and is absent in the human host, making this parasite enzyme an attractive target for antimalarial drug design. To characterize PfDXR, it is necessary to produce large quantities of the enzyme in a soluble and functional form. However, the over–production of malarial proteins in prokaryotic host systems often results in the formation of truncated proteins or insoluble protein aggregates. A heterologous expression system was developed for the production of active PfDXR using codon harmonization and tight control of expression in the presence of lac repressor. Yields of up to 2 mg/l of enzyme were reported using the optimised expression system, which is 8 to 10– fold greater than previously reported yields. The kinetic parameters Km, Vmax and kcat were determined for PfDXR; values reported in this study were consistent with those reported in the literature for other DXR enzymes. A three–dimensional model of the malarial drug target protein PfDXR was generated, and validated using structure–checking programs and protein docking studies. Structural and functional features unique to PfDXR were identified using the model and comparative sequence analyses with apicomplexan and non–apicomplexan DXR proteins. Residues Val44 and Asn45, essential for NADPH binding; and catalytic hatch residues Lys224 and Lys226, which are unique to the species of Plasmodium, were mutated to resemble those of E. coli DXR. Interestingly,these mutations resulted in significant reductions in substrate affinity, when compared to the unmutated PfDXR. Mutant enzymes PfDXR(VN43,44AG) and PfDXR(KK224,226NS) also demonstrated a decreased ability to turnover substrate by 4–fold and 2–fold respectively. This study indicates a difference in the role of the catalytic hatch of PfDXR with regards to the way in which it captures substrates. The study also highlights subtle differences in cofactor binding to PfDXR, compared with the well characterized EcDXR enzyme. The validated PfDXR model was also used to develop a novel efficient in silico screening method for potential tool compounds for use in the rational design of novel DXR inhibitors. Following in silico screening of 46 potential DXR inhibitors, a two–tiered in vitro screening approach was undertaken. DXR inhibition was assessed for the 46 novel compounds using an NADPH– ependant DXP enzyme inhibition assay and antimalarial potential was assessed using P.falciparum–infected erythrocyte growth assays. Select compounds were tested in human cells in order to determine whether they were toxic to the host. From the parallel in silico and in vitro drug screening, it was evident that only a single compound demonstrated reasonable potential binding to DXR (determined using in silico docking), inhibited DXR in vitro and inhibited P. falciparum growth, without being toxic to human cells. Its potential as a lead compound in antimalarial drug development is therefore feasible.…

Subjects/Keywords: Antimalarials – Development; Plasmodium falciparum; Drug development; Plasmodium falciparum – Purification; Plasmodium falciparum – Inhibitors; Enzyme kinetics; Malaria – Chemotherapy

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Goble, J. L. (2011). The druggable antimalarial target 1-deoxy-D-xylulose-5-phosphate reductoisomerase: purfication, kinetic characterization and inhibition studies. (Thesis). Rhodes University. Retrieved from http://hdl.handle.net/10962/d1004008

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Goble, Jessica Leigh. “The druggable antimalarial target 1-deoxy-D-xylulose-5-phosphate reductoisomerase: purfication, kinetic characterization and inhibition studies.” 2011. Thesis, Rhodes University. Accessed February 26, 2021. http://hdl.handle.net/10962/d1004008.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Goble, Jessica Leigh. “The druggable antimalarial target 1-deoxy-D-xylulose-5-phosphate reductoisomerase: purfication, kinetic characterization and inhibition studies.” 2011. Web. 26 Feb 2021.

Vancouver:

Goble JL. The druggable antimalarial target 1-deoxy-D-xylulose-5-phosphate reductoisomerase: purfication, kinetic characterization and inhibition studies. [Internet] [Thesis]. Rhodes University; 2011. [cited 2021 Feb 26]. Available from: http://hdl.handle.net/10962/d1004008.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Goble JL. The druggable antimalarial target 1-deoxy-D-xylulose-5-phosphate reductoisomerase: purfication, kinetic characterization and inhibition studies. [Thesis]. Rhodes University; 2011. Available from: http://hdl.handle.net/10962/d1004008

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

27. Martha Cecilia Suárez-Mutis. Epidemiologia da malária em comunidades do rio Padauiri, médio rio Negro, uma área de extrativismo vegetal da piaçaba no estado de Amazonas,Brasil.

Degree: 2007, Fundação Oswaldo Cruz

URL: http://www.bdtd.cict.fiocruz.br/tedesimplificado/tde_busca/arquivo.php?codArquivo=104

► A região do rio Padauiri, no médio rio Negro, Estado do Amazonas, é uma área de extração de piaçaba com alta incidência de malária cuja… (more)

▼ A região do rio Padauiri, no médio rio Negro, Estado do Amazonas, é uma área de extração de piaçaba com alta incidência de malária cuja epidemiologia local ainda não tinha sido estudada. Com o objetivo de avaliar a morbidade da malária nessa área foi desenhado um estudo observacional misto. Para conhecer a situação de base da malária e como não existia informação sistemática anterior foi realizado inicialmente um estudo retrospectivo, posteriormente um estudo piloto seccional para avaliar os antecedentes de malária, a partir do qual foi estabelecida uma coorte aberta durante 18 meses para acompanhar as pessoas que adquiriram infecção pelo Plasmódium e quem tinha feito quadro clínico de malária. As pessoas foram entrevistadas, submetidas a um exame físico direcionado aos sinais da malária e coletado sangue para realização de gota espessa, esfregaço de sangue periférico, PCR para diagnóstico molecular do parasita e sorologia para determinação de níveis de transmissão. Concomitantemente foram feitos estudos entomológicos procurando conhecer os principais vetores e seu comportamento assim como os prováveis criadouros naturais dos mesmos. Foi definido como caso de malária, toda pessoa com sintomas ou sinais da doença e com presença de plasmódios na gota espessa e como infecção assintomática toda pessoa com gota espessa positiva e/ou PCR positivo para qualquer uma das espécies do Plasmodium que não tivesse tido sintomas em até 30 dias antes e depois da coleta da amostra. Para classificar o caso como assintomático a pessoa não podia ter tomado medicamentos antimaláricos nos últimos 30 dias antes da coleta da amostra. Foram acompanhadas 188 pessoas. Durante o período de acompanhamento não houve óbitos nem internações devidas à malária na coorte estudada. No total, 53,2% das pessoas (100/188) tiveram malária com 169 episódios diferentes da doença. A incidência parasitária anual na área foi de 602,3 para cada 1000 habitantes. Houve uma média de 1,7 episódios de malária por pessoa sendo as crianças com menos de 5 anos aquelas que tiveram maior risco para contrair a doença quando comparadas com o resto da população (RR:1,64 IC95%:1,26-2,14, p=0,012); a média de episódios de malária nessas crianças foi de 2,2. Apesar da ocorrência dos dois tipos de Plasmodium mais comuns nas Américas, nos últimos anos há um predomínio de infecção pelo P. vivax na área, mostrando um padrão de mudança quando comparados com a década anterior. Foram encontrados casos de infecção assintomática por Plasmodium (tanto P. vivax quanto P. falciparum) em um percentual que flutuou entre 8,2 a 24%. Os episódios clínicos de malária foram sazonais e houve uma tendência a ocorrer no inicio do período das chuvas e no final das mesmas. A infecção assintomática, ao contrario foi mais freqüente durante o período da estação da seca. O principal transmissor encontrado foi o An. darlingi que apesar de ter sido coletado em todas as localidades, sua freqüência foi muito maior nas comunidades mais afastadas da desembocadura do rio Padauiri no rio Negro, nas áreas próximas… Advisors/Committee Members: José Rodrigues Coura.

Subjects/Keywords: Malária; Comunidades Pequenas; Plasmodium falciparum; Plasmodium vivax; DOENCAS INFECCIOSAS E PARASITARIAS; Malaria; Small Communities; Plasmodium falciparum; Plasmodium vivax

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Suárez-Mutis, M. C. (2007). Epidemiologia da malária em comunidades do rio Padauiri, médio rio Negro, uma área de extrativismo vegetal da piaçaba no estado de Amazonas,Brasil. (Thesis). Fundação Oswaldo Cruz. Retrieved from http://www.bdtd.cict.fiocruz.br/tedesimplificado/tde_busca/arquivo.php?codArquivo=104

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Suárez-Mutis, Martha Cecilia. “Epidemiologia da malária em comunidades do rio Padauiri, médio rio Negro, uma área de extrativismo vegetal da piaçaba no estado de Amazonas,Brasil.” 2007. Thesis, Fundação Oswaldo Cruz. Accessed February 26, 2021. http://www.bdtd.cict.fiocruz.br/tedesimplificado/tde_busca/arquivo.php?codArquivo=104.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Suárez-Mutis, Martha Cecilia. “Epidemiologia da malária em comunidades do rio Padauiri, médio rio Negro, uma área de extrativismo vegetal da piaçaba no estado de Amazonas,Brasil.” 2007. Web. 26 Feb 2021.

Vancouver:

Suárez-Mutis MC. Epidemiologia da malária em comunidades do rio Padauiri, médio rio Negro, uma área de extrativismo vegetal da piaçaba no estado de Amazonas,Brasil. [Internet] [Thesis]. Fundação Oswaldo Cruz; 2007. [cited 2021 Feb 26]. Available from: http://www.bdtd.cict.fiocruz.br/tedesimplificado/tde_busca/arquivo.php?codArquivo=104.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Suárez-Mutis MC. Epidemiologia da malária em comunidades do rio Padauiri, médio rio Negro, uma área de extrativismo vegetal da piaçaba no estado de Amazonas,Brasil. [Thesis]. Fundação Oswaldo Cruz; 2007. Available from: http://www.bdtd.cict.fiocruz.br/tedesimplificado/tde_busca/arquivo.php?codArquivo=104

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

28. Gaillard, Tiphaine. Identification et validation de marqueurs moléculaires de la résistance de Plasmodium falciparum à la doxycycline : Identification and validation of molecular markers of resistance of plasmodium falciparum to doxycycline.

Degree: Docteur es, Pathologie humaine. Maladies infectieuses, 2015, Aix Marseille Université

URL: http://www.theses.fr/2015AIXM5036

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La doxycycline est l’une des molécules recommandées par l’OMS en prophylaxie pour les voyageurs dans les zones d’endémie palustre, en particulier dans les zones de… (more)

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La doxycycline est l’une des molécules recommandées par l’OMS en prophylaxie pour les voyageurs dans les zones d’endémie palustre, en particulier dans les zones de multirésistance. Une étude récente avait suggéré que les isolats de P. falciparum présentaient différents niveaux de sensibilité à la doxycycline et que l’augmentation du nombre de copies de deux gènes, pfmdt ou pftetQ, pouvait être associée à une baisse de sensibilité.Le premier objectif de ce travail a consisté à valider ce modèle à partir d’un nouvel échantillonnage d’isolats africains. Le second objectif était d’évaluer le nombre de copies de ces deux gènes sur des isolats originaires de Thaïlande. Le troisième objectif a consisté à rechercher d’autres sources de résistance en investiguant le polymorphisme des gènes codant l’ARN ribosomal plasmodial potentiellement impliqués dans la résistance in vitro à la doxycycline.Les résultats nous ont permis de confirmer que le nombre de copies des gènes pfmdt ou pftetQ pouvait être impliqué dans la résistance in vitro à la doxycycline en Afrique. Les résultats concernant les isolats Thaï n’ont pas permis de corréler le nombre de copies des gènes pfmdt et pftetQ au phénotype CI50. Ces éléments montrent que ce mécanisme de résistance seul est insuffisant pour expliquer la résistance à la doxycycline ; les résultats sont en faveur d’une résistance médiée par plusieurs gènes.La recherche de points de mutation sur le gène pfssrRNA codant pour la petite sous-unité ribosomale de l’ADN plasmodial n’a pas abouti. D’autres cibles moléculaires sont en cours d’étude pour expliquer les mécanismes de résistance de P. falciparum à la doxycycline.

Doxycycline is currently one of the recommended chemoprophylactic regimens for travellers visiting malaria-endemic, particularly in countries with a high prevalence of resistance to chloroquine and multiresistance. A previous study suggested that increased pfmdt or pftetQ copy number could be associated with a lower susceptibility to doxycycline.The first aim of this study was to validate the pre-established model involving these two molecular markers with other African isolates. The second was to evaluate these markers in P. falciparum isolates coming from a multiresistance area in Thaïland. The third was to investigate the eventual association between the polymorphism in genes encoding ribosomal rRNA and in vitro resistance to doxycycline.The results confirm that pfmdt or pftetQ copy numbers should be involved in in vitro susceptibility to doxycycline in African P. falciparum isolates. The results concerning the Thai isolates indicate that there is no correlation between the pfmdt and pftetQ genes copy numbers and the belonging to the high doxycycline IC50 phenotype; this implies that this mechanism of resistance is not enough by itself to explain resistance to doxycycline; it augurs that the resistance to doxycycline should be controlled by multiple genes, and that these genetic markers could be continent-dependent. The search for points of mutation in isolates from the…

Advisors/Committee Members: Pradines, Bruno (thesis director).

Subjects/Keywords: Plasmodium; Plasmodium falciparum; Marqueurs moléculaires; Doxycycline; Tétracyclines; Macrolides; Antibiotiques; Plasmodium; Plasmodium falciparum; Molecular markers; Doxycycline; Tetracyclines; Macrolides; Antibiotics

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Gaillard, T. (2015). Identification et validation de marqueurs moléculaires de la résistance de Plasmodium falciparum à la doxycycline : Identification and validation of molecular markers of resistance of plasmodium falciparum to doxycycline. (Doctoral Dissertation). Aix Marseille Université. Retrieved from http://www.theses.fr/2015AIXM5036

Chicago Manual of Style (16^th Edition):

Gaillard, Tiphaine. “Identification et validation de marqueurs moléculaires de la résistance de Plasmodium falciparum à la doxycycline : Identification and validation of molecular markers of resistance of plasmodium falciparum to doxycycline.” 2015. Doctoral Dissertation, Aix Marseille Université. Accessed February 26, 2021. http://www.theses.fr/2015AIXM5036.

MLA Handbook (7^th Edition):

Gaillard, Tiphaine. “Identification et validation de marqueurs moléculaires de la résistance de Plasmodium falciparum à la doxycycline : Identification and validation of molecular markers of resistance of plasmodium falciparum to doxycycline.” 2015. Web. 26 Feb 2021.

Vancouver:

Gaillard T. Identification et validation de marqueurs moléculaires de la résistance de Plasmodium falciparum à la doxycycline : Identification and validation of molecular markers of resistance of plasmodium falciparum to doxycycline. [Internet] [Doctoral dissertation]. Aix Marseille Université 2015. [cited 2021 Feb 26]. Available from: http://www.theses.fr/2015AIXM5036.

Council of Science Editors:

Gaillard T. Identification et validation de marqueurs moléculaires de la résistance de Plasmodium falciparum à la doxycycline : Identification and validation of molecular markers of resistance of plasmodium falciparum to doxycycline. [Doctoral Dissertation]. Aix Marseille Université 2015. Available from: http://www.theses.fr/2015AIXM5036

Université Montpellier II

29. Curra, Chiara. Protein trafficking and host cell remodeling in malaria parasite infection : Le trafic des protéines et le remodelage de la cellule hôte dans l'infection par le parasite du paludisme.

Degree: Docteur es, Biochimie et biologie moléculaire, 2010, Université Montpellier II

URL: http://www.theses.fr/2010MON20219

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Pour assurer ses besoins de croissance, multiplication, et survie, Plasmodium modifie sa cellule hôte, l'érythrocyte, après l'invasion. Le parasite met en place ainsi un système… (more)

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Pour assurer ses besoins de croissance, multiplication, et survie, Plasmodium modifie sa cellule hôte, l'érythrocyte, après l'invasion. Le parasite met en place ainsi un système d'échanges (import/export) avec sa cellule hôte et le milieu extérieur. Nous avons identifié dans la base de données de Plasmodium berghei, le parasite de rongeurs, une famille de gènes, sep, correspondant à la famille etramp chez Plasmodium falciparum. Cette famille de gènes code pour des petites protéines exportées, et conservées dans tout le genre Plasmodium. Les protéines SEP (13?16 kDa) contiennent en N-terminal un peptide signal prédit, un domaine hydrophobe interne, et elles diffèrent au niveau des régions C-terminal et 3' UTR. Toutefois, les protéines SEP sont exprimées à différents moments du cycle de Plasmodium. Durant le cycle érythrocytaire, PbSEP1 et PbSEP3 sont exprimées à partir du stade trophozoïte, et la même quantité de protéine est détectée au stade schizonte et gamétocyte, pendant que PbSEP3 est hautement détectée dans les trophozoïtes mûrs et les gamétocytes. Chez le moustique, PbSEP1 et PbSEP3 sont détectées seulement chez les ookinètes, alors que PbSEP2 est très abondante dans les ookinètes, oocystes, et sporozoïtes des glandes salivaires. Les protéines SEP ont également des localisations différentes. Dans l'érythrocyte, PbSEP1 est localisée dans la membrane de la vacuole parasitophore, alors que PbSEP2 et PbSEP3 sont exportées au-delà de cette vacuole, et sont ainsi localisées dans la cellule hôte, en association avec des structures vésiculaires. Dans cette étude, nous avons identifié les signaux d'adressage des protéines SEP dans la vacuole parasitophore et dans la cellule hôte, chez Plasmodium berghei. L'autre partie du travail, effectuée à l'Université de Montpellier II, a consisté à étudier la localisation de deux protéines du squelette sous- membranaire de l'érythrocyte, la dématine, et l'adducine, durant le développement intra-érythrocytaire de Plasmodium falciparum. Le but de cette étude étant d'identifier un mécanisme potentiel d'internalisation des composants du squelette sous-membranaire de l'érythrocyte dans le parasite. Des études d'immuno-localisation ont montré que la dématine et l'adducine sont internalisées à partir du stade trophozoïte, et sont localisées probablement à la vacuole parasitophore (membrane et/ou lumière). Cette internalisation a été confirmée par des études de fractionnement cellulaire et d'accessibilité à la protéinase K, montrant que la dématine est totalement internalisée, alors l'adducine ne l'est que partiellement, suggérant une localisation de la protéine à la périphérie du parasite.

Plasmodium endurance depends on the ability of the parasite to reorganize the cytosol of the erythrocyte, a terminally differentiated cell, and remodel its skeleton membrane immediately after invasion. In this way the parasite can organize the import/export of the molecules necessary to its survival. The comprehension of cellular trafficking mechanisms which occur during Plasmodium infection is a…

Advisors/Committee Members: Braun-Breton, Catherine (thesis director), Sinibaldi Vallebona, Paola (thesis director).

Subjects/Keywords: Plasmodium berghei; Paludisme; Plasmodium falciparum; Paludisme; Malaria; Exported proteins; Erythrocyte remodeling; Cellular trafficking; Plasmodium berghei; Plasmodium falciparum

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Curra, C. (2010). Protein trafficking and host cell remodeling in malaria parasite infection : Le trafic des protéines et le remodelage de la cellule hôte dans l'infection par le parasite du paludisme. (Doctoral Dissertation). Université Montpellier II. Retrieved from http://www.theses.fr/2010MON20219

Chicago Manual of Style (16^th Edition):

Curra, Chiara. “Protein trafficking and host cell remodeling in malaria parasite infection : Le trafic des protéines et le remodelage de la cellule hôte dans l'infection par le parasite du paludisme.” 2010. Doctoral Dissertation, Université Montpellier II. Accessed February 26, 2021. http://www.theses.fr/2010MON20219.

MLA Handbook (7^th Edition):

Curra, Chiara. “Protein trafficking and host cell remodeling in malaria parasite infection : Le trafic des protéines et le remodelage de la cellule hôte dans l'infection par le parasite du paludisme.” 2010. Web. 26 Feb 2021.

Vancouver:

Curra C. Protein trafficking and host cell remodeling in malaria parasite infection : Le trafic des protéines et le remodelage de la cellule hôte dans l'infection par le parasite du paludisme. [Internet] [Doctoral dissertation]. Université Montpellier II; 2010. [cited 2021 Feb 26]. Available from: http://www.theses.fr/2010MON20219.

Council of Science Editors:

Curra C. Protein trafficking and host cell remodeling in malaria parasite infection : Le trafic des protéines et le remodelage de la cellule hôte dans l'infection par le parasite du paludisme. [Doctoral Dissertation]. Université Montpellier II; 2010. Available from: http://www.theses.fr/2010MON20219

University of Pretoria

30. [No author]. In Vitro Medicinal Properties of Novel Compounds from Croton steenkampianus .

Degree: 2009, University of Pretoria

URL: http://upetd.up.ac.za/thesis/available/etd-05242009-123335/

► The effect of infectious diseases on the population in the developing countries is of utmost concern. Malaria, tuberculosis (TB) and human immunodeficiency virus (HIV) are… (more)

▼ The effect of infectious diseases on the population in the developing countries is of utmost concern. Malaria, tuberculosis (TB) and human immunodeficiency virus (HIV) are the three major infectious disease threats. They account for approximately half of the mortality caused by infectious diseases, which is almost half of the mortality in the developing countries. With no vaccine likely in the foreseeable future, drugs remain the best means of controlling infectious diseases. In the industrialized nations at the present time, some 50% of all prescribed drugs are derived or synthesized from natural products (animals, marine species, plants and micro-organisms). It has been estimated that plants are the most important source of medicine for more than 80% of the world’s population. As previous work on the leaves of Croton steenkampianus gave promising results and revealed that it still contained bioactive compounds that could be isolated, it was chosen for further work. The bioassay guided fractionation of the ethanol crude extract using silica and Sephadex column chromatography resulted in the isolation of six compounds: three flavoniods (quercetin, tamarixetin and eriodictyol), one new indane (1) (2,6-dimethyl-1-oxo-4 indanecarboxylic acid) and two new diterpenes (steenkrotin A (2) and steenkrotin B (3)) with novel skeletons. The structure of the compounds was determined using NMR, IR, UV, MS and X-ray crystallography. Ethanol crude extract, quercetin, steenkrotin A, steenkrotin B and the indane were tested against four strains of Plasmodium falciparum (D6, D10, Dd2 and W2). Quercetin showed good antiplasmodial activity against the D10 and Dd2 strains. The antiplasmodial activity of steenkrotin A and crude extract were moderate. The antimalarial activity of steenkrotin A in particular is promising, as it showed more activity against resistant strains. The indane, and steekrotin B were not active against the strains of P. falciparum used (IC50 > 10 μg/m). The IC50 of the compounds improved when they were combined with chloroquine. However, the IC50 of chloroquine was still the lowest. The compounds showed moderate bioactivity against Bacillus cereus and Escherichia coli. The three new compounds (1, 2 and 3) tested against Mycobacterium (H37Rv) were not active (IC50 > 10 μg/ml). The indane (1) showed anti-HIV activity at 50 μg/ml against reverse transcriptase. The antioxidant activity of the compounds tested ranged from weak to excellent (>280.00 μg/ml for compound 1 and 2 to 0.05 μg/ml for quercetin). The cytotoxicity of the compounds and extract were determined against Vero cells lines. Their IC50 values ranged from 34.0 to 305.9 ìg/ml, which is higher and better than that of chloroquine. The IC50 values obtained are: chloroquine (25.0), quercetin (33.6), steenkrotin A (35.0), ethanol extract (45.0), tamarixetin (53.8), indane (248.2) and steenkrotin B (305.9). Advisors/Committee Members: Prof J J M Meyer (advisor).

Subjects/Keywords: Croton steenkampianus; Infectious diseases; Plasmodium falciparum; UCTD

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

author], [. (2009). In Vitro Medicinal Properties of Novel Compounds from Croton steenkampianus . (Doctoral Dissertation). University of Pretoria. Retrieved from http://upetd.up.ac.za/thesis/available/etd-05242009-123335/

Chicago Manual of Style (16^th Edition):

author], [No. “In Vitro Medicinal Properties of Novel Compounds from Croton steenkampianus .” 2009. Doctoral Dissertation, University of Pretoria. Accessed February 26, 2021. http://upetd.up.ac.za/thesis/available/etd-05242009-123335/.

MLA Handbook (7^th Edition):

author], [No. “In Vitro Medicinal Properties of Novel Compounds from Croton steenkampianus .” 2009. Web. 26 Feb 2021.

Vancouver:

author] [. In Vitro Medicinal Properties of Novel Compounds from Croton steenkampianus . [Internet] [Doctoral dissertation]. University of Pretoria; 2009. [cited 2021 Feb 26]. Available from: http://upetd.up.ac.za/thesis/available/etd-05242009-123335/.

Council of Science Editors:

author] [. In Vitro Medicinal Properties of Novel Compounds from Croton steenkampianus . [Doctoral Dissertation]. University of Pretoria; 2009. Available from: http://upetd.up.ac.za/thesis/available/etd-05242009-123335/

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Open Access Theses and Dissertations