You searched for subject:(Plasmodium falciparum).
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University of Zambia
1.
Masase, Charles Chomba.
Contribution of plasmodium falciparun and other infections to acute central nervous system disease at the University Teaching Hospital of Lusaka
.
Degree: 2012, University of Zambia
URL: http://hdl.handle.net/123456789/1484 
► Three hundred patients presenting with acute central nervous system (CNS) disease were enrolled in this one-year study from June 1997 to June 1998. This was…
(more)
▼ Three hundred patients presenting with acute central nervous system (CNS) disease were enrolled in this one-year study from June 1997 to June 1998. This was a longitudinal prospective study looking at the contribution of Plasmodium falciparum and other infections to acute central nervous system disease. It also looked at the clinical and laboratory features of patients presenting with acute CNS disease.
The clinical features were vast though most of the patients had a headache (84%), fever (70%) and confusion (40%). The other symptoms included convulsions (17%), weight loss (31%), diarrhoea (40%) while 34% of the patients had given a history of vomiting. The major signs were fever (87%), wasting (64%), meningeal signs (62%), lymphadenopathy (50%), papilloedema (19%), anaemia (54%) and 44% had non-specific macular popular dermatosis.
The study has demonstrated that Plasmodium falciparum is an important cause of acute CNS diseases as 20% of the patients in this study had positive malaria parasite slides. The contribution of other infections were mainly bacteria and it has been shown that Cryptoccoccal neoformans (6.7%), Streptococcus pneumoniae (3.3%), Neisseria meningitides (2.7%), Haemophilus influenzae (0.7%), other bacteria (6.7%) are important causes of acute central nervous system disease. This study population, which had a 1:1 male to female ratio, had a high HIV seroprevalence rate of 75%. The biochemical results did not appear to be helpful except in situations were patients had hypoglycaemia. The RPR results were positive in only 3.3% of the study population. No acid alcohol bacilli were seen or isolated.
The study has demonstrated that Plasmodium falciparum and other infections are an important cause of acute CNS diseases and should therefore always be considered in the management of affected individuals. The range of bacteria pathogens has increased with HIV related infections being common though there was limitation on the range of viral infections.
Subjects/Keywords: Plasmodium;
Plasmodium Falciparum
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APA (6th Edition):
Masase, C. C. (2012). Contribution of plasmodium falciparun and other infections to acute central nervous system disease at the University Teaching Hospital of Lusaka
. (Thesis). University of Zambia. Retrieved from http://hdl.handle.net/123456789/1484
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Masase, Charles Chomba. “Contribution of plasmodium falciparun and other infections to acute central nervous system disease at the University Teaching Hospital of Lusaka
.” 2012. Thesis, University of Zambia. Accessed February 22, 2020.
http://hdl.handle.net/123456789/1484.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Masase, Charles Chomba. “Contribution of plasmodium falciparun and other infections to acute central nervous system disease at the University Teaching Hospital of Lusaka
.” 2012. Web. 22 Feb 2020.
Vancouver:
Masase CC. Contribution of plasmodium falciparun and other infections to acute central nervous system disease at the University Teaching Hospital of Lusaka
. [Internet] [Thesis]. University of Zambia; 2012. [cited 2020 Feb 22].
Available from: http://hdl.handle.net/123456789/1484.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Masase CC. Contribution of plasmodium falciparun and other infections to acute central nervous system disease at the University Teaching Hospital of Lusaka
. [Thesis]. University of Zambia; 2012. Available from: http://hdl.handle.net/123456789/1484
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
University of Georgia
2.
Altharawi, Ali Ibrahim.
Design and synthesis of NADH mimics that target mitochondrial electron transport of plasmodium parasite.
Degree: MS, Pharmacy, 2013, University of Georgia
URL: http://purl.galileo.usg.edu/uga_etd/altharawi_ali_i_201308_ms
► Malaria, particularly that caused by Plasmodium falciparum, remains one of the most deadly infectious diseases worldwide. Unfortunately, multi–drug resistance (MDR) to currently used antimalarials has…
(more)
▼ Malaria, particularly that caused by
Plasmodium falciparum, remains one of the most deadly infectious diseases worldwide. Unfortunately, multi–drug resistance (MDR) to currently used antimalarials has spread worldwide and led to a failure to control malaria. Therefore, new chemotherapeutic agents that are affordable, easy to synthesize, and overcome resistant strains of parasites are urgently needed.
Plasmodium mitochondria has attracted the attention of many projects as a potential target for malaria treatment, and provided atovaquone as treatment and prophylaxis of malaria.
In this project, the advantage of a triphenylphosphonium (TPP) moiety in delivering biomolecules to mitochondria has been investigated to deliver NADH-mimic compounds as potential inhibitors of the mitochondrial electron transport chain (mtETC). A small library of phosphonium cations based on clopidal and amodiaquine (AQ) were synthesized and evaluated for antiparasitic activity against W2 chloroquine-resistant strain. The test results revealed that the phosphonium moiety reduced antiplasmodial efficacy, and phosphonium substituents (R = Me) were as effective as (TPP) moiety. It is hypothesized that re-routing the AQ-based phosphonium cation to the negatively-charged mitochondria by electrostatic attraction resulted in moderate antiplasmodial effects and the overall activities of the compounds in this study were the result of NADH dehydrogenase (Complex I) inhibition.
Advisors/Committee Members: J. Warren Beach.
Subjects/Keywords: Plasmodium falciparum
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APA (6th Edition):
Altharawi, A. I. (2013). Design and synthesis of NADH mimics that target mitochondrial electron transport of plasmodium parasite. (Masters Thesis). University of Georgia. Retrieved from http://purl.galileo.usg.edu/uga_etd/altharawi_ali_i_201308_ms
Chicago Manual of Style (16th Edition):
Altharawi, Ali Ibrahim. “Design and synthesis of NADH mimics that target mitochondrial electron transport of plasmodium parasite.” 2013. Masters Thesis, University of Georgia. Accessed February 22, 2020.
http://purl.galileo.usg.edu/uga_etd/altharawi_ali_i_201308_ms.
MLA Handbook (7th Edition):
Altharawi, Ali Ibrahim. “Design and synthesis of NADH mimics that target mitochondrial electron transport of plasmodium parasite.” 2013. Web. 22 Feb 2020.
Vancouver:
Altharawi AI. Design and synthesis of NADH mimics that target mitochondrial electron transport of plasmodium parasite. [Internet] [Masters thesis]. University of Georgia; 2013. [cited 2020 Feb 22].
Available from: http://purl.galileo.usg.edu/uga_etd/altharawi_ali_i_201308_ms.
Council of Science Editors:
Altharawi AI. Design and synthesis of NADH mimics that target mitochondrial electron transport of plasmodium parasite. [Masters Thesis]. University of Georgia; 2013. Available from: http://purl.galileo.usg.edu/uga_etd/altharawi_ali_i_201308_ms
University of Georgia
3.
Stokes, John Vincent.
Functional characterization of heterologously expressed Plasmodium falciparum ebl proteins.
Degree: MS, Infectious Diseases, 2007, University of Georgia
URL: http://purl.galileo.usg.edu/uga_etd/stokes_john_v_200712_ms
► While heterologous expression has replaced biochemical purification as the principle method for obtaining purified, authentic proteins for characterization, no single expression system has proven appropriate…
(more)
▼ While heterologous expression has replaced biochemical purification as the principle method for obtaining purified, authentic proteins for characterization, no single expression system has proven appropriate for all applications. Heterologous expression of
Plasmodium falciparum genes has proven especially challenging due to their extreme A+T codon bias and inappropriate post-translational modifications to product made by existing expression systems. These issues have generated interest in developing a novel heterologous expression system for P.
falciparum. Here, we have transfected the ciliate Tetrahymena thermophila with a novel secretion vector bearing the R2 binding region of the P.
falciparum genes, ebl-1 and jsebl. In addition, we demonstrated successful expression of the R2 region. This is the first demonstration of secreted P.
falciparum antigens expressed by T. thermophila. We believe T. thermophila shows promise as a heterologous expression system for P.
falciparum genes.
Advisors/Committee Members: David Peterson.
Subjects/Keywords: Plasmodium falciparum
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APA (6th Edition):
Stokes, J. V. (2007). Functional characterization of heterologously expressed Plasmodium falciparum ebl proteins. (Masters Thesis). University of Georgia. Retrieved from http://purl.galileo.usg.edu/uga_etd/stokes_john_v_200712_ms
Chicago Manual of Style (16th Edition):
Stokes, John Vincent. “Functional characterization of heterologously expressed Plasmodium falciparum ebl proteins.” 2007. Masters Thesis, University of Georgia. Accessed February 22, 2020.
http://purl.galileo.usg.edu/uga_etd/stokes_john_v_200712_ms.
MLA Handbook (7th Edition):
Stokes, John Vincent. “Functional characterization of heterologously expressed Plasmodium falciparum ebl proteins.” 2007. Web. 22 Feb 2020.
Vancouver:
Stokes JV. Functional characterization of heterologously expressed Plasmodium falciparum ebl proteins. [Internet] [Masters thesis]. University of Georgia; 2007. [cited 2020 Feb 22].
Available from: http://purl.galileo.usg.edu/uga_etd/stokes_john_v_200712_ms.
Council of Science Editors:
Stokes JV. Functional characterization of heterologously expressed Plasmodium falciparum ebl proteins. [Masters Thesis]. University of Georgia; 2007. Available from: http://purl.galileo.usg.edu/uga_etd/stokes_john_v_200712_ms
4.
ROSIMEIRE CRISTINA DALLA MARTHA.
ANÁLISE DA VARIAÇÃO DE 11 MARCADORES DE MICROSSATÉLITES DE Plasmodium falciparum EM PACIENTES SINTOMÁTICOS E ASSINTOMÁTICOS DE UMA COMUNIDADE EM ZONA ENDEMICA DE MALÁRIA DE PORTO VELHO-RO.
Degree: 2005, Fundação Universidade Federal de Rondônia
URL: http://www.bdtd.unir.br/tde_busca/arquivo.php?codArquivo=29
► No presente trabalho foram estudados os genótipos de Plasmodium falciparum de indivíduos residentes em localidades distintas da cidade de Porto Velho (Vila de Candelária, Bate-…
(more)
▼ No presente trabalho foram estudados os genótipos de Plasmodium falciparum de indivíduos residentes em localidades distintas da cidade de Porto Velho (Vila de Candelária, Bate- Estaca e diversos bairros da cidade). Quarenta e quatro amostras, divididas em cinco grupos: dois de indivíduos assintomáticos (em épocas de coletas diferentes e pertencentes da mesma localidade) e três sintomáticos (Vila de Candelária, Bate Estaca, e do atendimento do CEPEM), foram analisadas através de 11 marcadores de microssatélites. Para cada grupo distinto foi verificado o índice de diversidade, o Fst (índice de fixação) e a porcentagem de infecções múltiplas presentes em cada grupo e correlacionado com os demais. A presença de infecções múltiplas foi observada em todos os grupos estudados, ressaltando os pacientes assintomáticos, que também apresentaram maior número de alelos entre os demais grupos. O grupo Cepem obteve o maior índice de heterozigosidade, e entre os grupos sintomáticos e assintomáticos de uma mesma localidade, não se observa uma diferença considerável. O Fst entre os grupos assintomáticos de Candelária e sintomáticos de Bate Estaca (Fst=0.37) demonstraram maior diferenciação genética, apesar da proximidade geográfica das localidades. Este estudo confirma que não existem genótipos, caracterizados por determinadas combinações de tamanhos de microssatelites, que causam infecções sintomáticas ou assintomáticas. O motivo de uma infecção virar sintomática aparentemente está possivelmente mais associado à expressão diferencial de fatores associados a virulência.
In the present study, genotypes of Plasmodium falciparum parasites obtained from residents of different suburbs of Porto Velho were compared by means of microsatellite analysis. Fourty-four samples, divided in five groups were analyzed: two groups of samples from non-symptomatic infections (Candelaria, taken at different timepoints) and three groups of samples from symptomatic patients (from Candelaria, Bate-Estaca and CEPEM) were analyzed using eleven different microsatellite markers. For each distinct group, the diversity index, the fixation index (Fst-value) and the percentage of mixed (polyclonal) infections. All groups presented multiple infections to a similar degree. As expected, the group of samples from CEPEM showed the highest degree of heterozigosity, since samples were from patients of several different locations. There was no significant difference in heterozygosity between samples from asymptomatic and symptomatic infections of the same location. The largest genetic differentiation was observed between Candelária (asymptomatics) and Bate Estaca (symptomatics) samples, in spite of the geographical proximity of the two locations. This study confirms that the rather similar circulating Plasmodium falciparum genotypes, determined by microsatellite analysis, can either both cause symptomatic or asymptomatic infections and that the reason for this probably lies in the differential expression of virulence associated genes.
Advisors/Committee Members: Luiz Hildebrando Pereira da Silva.
Subjects/Keywords: BIOLOGIA GERAL; Plasmodium falciparum; Plasmodium falciparum
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APA (6th Edition):
MARTHA, R. C. D. (2005). ANÁLISE DA VARIAÇÃO DE 11 MARCADORES DE MICROSSATÉLITES DE Plasmodium falciparum EM PACIENTES SINTOMÁTICOS E ASSINTOMÁTICOS DE UMA COMUNIDADE EM ZONA ENDEMICA DE MALÁRIA DE PORTO VELHO-RO. (Thesis). Fundação Universidade Federal de Rondônia. Retrieved from http://www.bdtd.unir.br/tde_busca/arquivo.php?codArquivo=29
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
MARTHA, ROSIMEIRE CRISTINA DALLA. “ANÁLISE DA VARIAÇÃO DE 11 MARCADORES DE MICROSSATÉLITES DE Plasmodium falciparum EM PACIENTES SINTOMÁTICOS E ASSINTOMÁTICOS DE UMA COMUNIDADE EM ZONA ENDEMICA DE MALÁRIA DE PORTO VELHO-RO.” 2005. Thesis, Fundação Universidade Federal de Rondônia. Accessed February 22, 2020.
http://www.bdtd.unir.br/tde_busca/arquivo.php?codArquivo=29.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
MARTHA, ROSIMEIRE CRISTINA DALLA. “ANÁLISE DA VARIAÇÃO DE 11 MARCADORES DE MICROSSATÉLITES DE Plasmodium falciparum EM PACIENTES SINTOMÁTICOS E ASSINTOMÁTICOS DE UMA COMUNIDADE EM ZONA ENDEMICA DE MALÁRIA DE PORTO VELHO-RO.” 2005. Web. 22 Feb 2020.
Vancouver:
MARTHA RCD. ANÁLISE DA VARIAÇÃO DE 11 MARCADORES DE MICROSSATÉLITES DE Plasmodium falciparum EM PACIENTES SINTOMÁTICOS E ASSINTOMÁTICOS DE UMA COMUNIDADE EM ZONA ENDEMICA DE MALÁRIA DE PORTO VELHO-RO. [Internet] [Thesis]. Fundação Universidade Federal de Rondônia; 2005. [cited 2020 Feb 22].
Available from: http://www.bdtd.unir.br/tde_busca/arquivo.php?codArquivo=29.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
MARTHA RCD. ANÁLISE DA VARIAÇÃO DE 11 MARCADORES DE MICROSSATÉLITES DE Plasmodium falciparum EM PACIENTES SINTOMÁTICOS E ASSINTOMÁTICOS DE UMA COMUNIDADE EM ZONA ENDEMICA DE MALÁRIA DE PORTO VELHO-RO. [Thesis]. Fundação Universidade Federal de Rondônia; 2005. Available from: http://www.bdtd.unir.br/tde_busca/arquivo.php?codArquivo=29
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
University of Zambia
5.
Hawela, Moonga B.
Determination of plasmodium falciparum parasites diversity using MSPII family specific allelic primers among symptomatic children aged five years and below in Zambia
.
Degree: 2012, University of Zambia
URL: http://hdl.handle.net/123456789/1320
► Introduction: Malaria is still a major public health problem in many tropical and subtropical countries. Therefore effective integrated malaria control strategies are a requirement. However,…
(more)
▼ Introduction: Malaria is still a major public health problem in many tropical and subtropical countries. Therefore effective integrated malaria control strategies are a requirement. However, parasite antigen diversity might be a hurdle in the efficacy of some malaria control strategies like the malaria vaccine against Plasmodium falciparum infection if a full repertoire of variant forms is not incorporated into the vaccine. In addition, malaria rapid diagnostic tests (RDTs) offer great potential for rapid and accurate diagnosis of malaria infections. Parasite genetic diversity of target antigens particularly for PfEiRP2-based RDTs may affect their sensitivity among other factors. Methods: In 2006, a prospective analytical study was conducted to determine the diversity oi Plasmodium falciparum merozoite surface protein 2 (MSP-2) in four sites in Zambia. A nested polymerase chain reaction (PCR) amplification was carried out on two hundred and eighty five dried blood spots with Plasmodium falciparum mono infection using primers for the conserved regions of MSP-2 as well as family specific alleles for the variable regions. PCR-restriction fragment length polymorphism analysis of MSP-2 using a Hinfl digests of each nested PCR product followed by product detection on a 2.0% agarose gel electrophoresis with ethidium bromide was performed to determine the allelic families and genotypes.
Results: The study findings show that, there are forty six different genotypes of Plasmodium falciparum commonly found in Zambia, twenty four allelic genotypes belong to the FC27 allelic family and twenty two fi-om the 3D7 allelic family. The study also indicated that Plasmodium falciparum infections carry more than one allelic genotype at each particular time and one infection carried eight different genotypes. Discussion: These findings show that Plasmodium falciparum in Zambia exists in many different allelic genotypes and several patients infected with Plasmodium falciparum carry atleast more than one genotype.
Conclusions: This means that the inclusion of all the available genotypes in both the Malaria vaccine and RDTs would be of maximum benefit to the Zambian populations. The multiple Plasmodium falciparum infections will have other implications such as having one genotype sensitive the drug and another resistant therefore this needs to be further explored.
Subjects/Keywords: Plasmodium falciparum;
Malaria
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APA (6th Edition):
Hawela, M. B. (2012). Determination of plasmodium falciparum parasites diversity using MSPII family specific allelic primers among symptomatic children aged five years and below in Zambia
. (Thesis). University of Zambia. Retrieved from http://hdl.handle.net/123456789/1320
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Hawela, Moonga B. “Determination of plasmodium falciparum parasites diversity using MSPII family specific allelic primers among symptomatic children aged five years and below in Zambia
.” 2012. Thesis, University of Zambia. Accessed February 22, 2020.
http://hdl.handle.net/123456789/1320.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Hawela, Moonga B. “Determination of plasmodium falciparum parasites diversity using MSPII family specific allelic primers among symptomatic children aged five years and below in Zambia
.” 2012. Web. 22 Feb 2020.
Vancouver:
Hawela MB. Determination of plasmodium falciparum parasites diversity using MSPII family specific allelic primers among symptomatic children aged five years and below in Zambia
. [Internet] [Thesis]. University of Zambia; 2012. [cited 2020 Feb 22].
Available from: http://hdl.handle.net/123456789/1320.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Hawela MB. Determination of plasmodium falciparum parasites diversity using MSPII family specific allelic primers among symptomatic children aged five years and below in Zambia
. [Thesis]. University of Zambia; 2012. Available from: http://hdl.handle.net/123456789/1320
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Latrobe University
6.
Abu Bakar, Nurhidanatasha.
Endocytic and digestive processes in Plasmodium falciparum and their role in artemisinin action.
Degree: PhD, 2011, Latrobe University
URL: http://hdl.handle.net/1959.9/492830
► Thesis (Ph.D.) - La Trobe University, 2011
Submission note: "A thesis submitted in total fulfilment of the requirements for the degree of Doctor of Philosophy,…
(more)
▼ Thesis (Ph.D.) - La Trobe University, 2011
Submission note: "A thesis submitted in total fulfilment of the requirements for the degree of Doctor of Philosophy, [to the] Department of Biochemistry, La Trobe Institute for Molecular Science, Faculty of Science, Technology and Engineering, La Trobe University, Bundoora."
Advisors/Committee Members: La Trobe University. Faculty of Science, Technology and Engineering., La Trobe University. La Trobe Institute for Molecular Science., La Trobe University. Department of Biochemistry..
Subjects/Keywords: Plasmodium falciparum.; Artemisinin.
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APA ·
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APA (6th Edition):
Abu Bakar, N. (2011). Endocytic and digestive processes in Plasmodium falciparum and their role in artemisinin action. (Doctoral Dissertation). Latrobe University. Retrieved from http://hdl.handle.net/1959.9/492830
Chicago Manual of Style (16th Edition):
Abu Bakar, Nurhidanatasha. “Endocytic and digestive processes in Plasmodium falciparum and their role in artemisinin action.” 2011. Doctoral Dissertation, Latrobe University. Accessed February 22, 2020.
http://hdl.handle.net/1959.9/492830.
MLA Handbook (7th Edition):
Abu Bakar, Nurhidanatasha. “Endocytic and digestive processes in Plasmodium falciparum and their role in artemisinin action.” 2011. Web. 22 Feb 2020.
Vancouver:
Abu Bakar N. Endocytic and digestive processes in Plasmodium falciparum and their role in artemisinin action. [Internet] [Doctoral dissertation]. Latrobe University; 2011. [cited 2020 Feb 22].
Available from: http://hdl.handle.net/1959.9/492830.
Council of Science Editors:
Abu Bakar N. Endocytic and digestive processes in Plasmodium falciparum and their role in artemisinin action. [Doctoral Dissertation]. Latrobe University; 2011. Available from: http://hdl.handle.net/1959.9/492830
University of Georgia
7.
Drummond, Paul Bassanio.
Identification and characterization of putative erythrocyte binding protein genes in Plasmodium falciparum.
Degree: PhD, Veterinary Parasitology, 2004, University of Georgia
URL: http://purl.galileo.usg.edu/uga_etd/drummond_paul_b_200405_phd
► The erythrocyte binding proteins (EBP) of Plasmodium falciparum are ligands that play a functional role in erythrocyte invasion by merozoites. Six EBP genes have already…
(more)
▼ The erythrocyte binding proteins (EBP) of
Plasmodium falciparum are ligands that play a functional role in erythrocyte invasion by merozoites. Six EBP genes have already been identified in the P.
falciparum genome. However, their role in invasion and potential interaction is not completely understood. A series of experimental studies were conducted to identify, and to characterize these EBP genes. A Polymerize Chain Reaction (PCR) approach was used to identify potential EBP genes in two P.
falciparum isolates. Several clones sequenced were known EBP genes, which validated our approach to identified novel members of the EBP family. However, research to determine the identity of these sequences is on going. A study was designed to investigate the level of sequence conservation within the Duffy binding-like domains of one member of the EBP family, the erythrocyte binding-like 1 (EBL-1) gene in ten P.
falciparum isolates. The result indicates a degree of polymorphism in the Duffy binding-like domains comparable to that observed for erythrocyte binding antigen-175 (a member of the EBP family). Additionally, there was a clear bias towards nonsynonymous substitution in the EBL-1 Duffy binding-like domains. Our result obtained from this study suggests that ebl-1 is not essential in some isolates. The results suggest that the diversity observed in the Duffy binding-like region of the ebl-1 gene in P.
falciparum may either be driven by host immune response, or may indicate receptor heterogeneity. The final goal of this work was a comprehensive study of EBP gene expression during the intraerythrocytic developmental cycle in three P.
falciparum isolates. Our results show that expression of EBPs is highly stage specific, with expression peaking at 36-hr in the 3D7 and Dd2 isolate, and at 44-hr in the Dd2-NM clone. We also found that eba-175 had the highest level of expression, while maebl was the least expressed in all three isolates. We also found one gene that is not expressed in all isolates.
Advisors/Committee Members: David S. Peterson.
Subjects/Keywords: Plasmodium falciparum
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APA (6th Edition):
Drummond, P. B. (2004). Identification and characterization of putative erythrocyte binding protein genes in Plasmodium falciparum. (Doctoral Dissertation). University of Georgia. Retrieved from http://purl.galileo.usg.edu/uga_etd/drummond_paul_b_200405_phd
Chicago Manual of Style (16th Edition):
Drummond, Paul Bassanio. “Identification and characterization of putative erythrocyte binding protein genes in Plasmodium falciparum.” 2004. Doctoral Dissertation, University of Georgia. Accessed February 22, 2020.
http://purl.galileo.usg.edu/uga_etd/drummond_paul_b_200405_phd.
MLA Handbook (7th Edition):
Drummond, Paul Bassanio. “Identification and characterization of putative erythrocyte binding protein genes in Plasmodium falciparum.” 2004. Web. 22 Feb 2020.
Vancouver:
Drummond PB. Identification and characterization of putative erythrocyte binding protein genes in Plasmodium falciparum. [Internet] [Doctoral dissertation]. University of Georgia; 2004. [cited 2020 Feb 22].
Available from: http://purl.galileo.usg.edu/uga_etd/drummond_paul_b_200405_phd.
Council of Science Editors:
Drummond PB. Identification and characterization of putative erythrocyte binding protein genes in Plasmodium falciparum. [Doctoral Dissertation]. University of Georgia; 2004. Available from: http://purl.galileo.usg.edu/uga_etd/drummond_paul_b_200405_phd
University of Pretoria
8.
Burger, Pieter Buys.
Development of a
dynamic receptor-based pharmacophore model of Plasmodium falciparum
spermidine synthase for selective inhibitor
identification.
Degree: Biochemistry, 2009, University of Pretoria
URL: http://hdl.handle.net/2263/24974
► Malaria affects the daily lives of more than 2 billion people worldwide and has been estimated to result in 300-500 million clinical cases annually leading…
(more)
▼ Malaria affects the daily lives of more than 2 billion
people worldwide and has been estimated to result in 300-500
million clinical cases annually leading to approximately 2 million
deaths, mainly caused by the most virulent malaria species,
Plasmodium falciparum. The lack of a vaccine and the rapid
emergence and spread of drug resistant strains of P.
falciparum,
necessitate the development of new antimalarials and the
identification and validation of new parasite-specific therapeutic
targets. Numerous studies directed at interfering with the
polyamine biosynthetic pathway in P.
falciparum have shown its
potential as a target for the development of a new class of
antimalarials. The essential nature of P.
falciparum spermidine
synthase (PfSpdSyn), an enzyme in the polyamine pathway of the
parasite warranted the further investigation to find novel lead
compounds. The high cost and attrition rate of drug discovery has
resulted in the implementation of smart drug discovery platforms in
both academia and industry. The strategy implemented in this study
involved the development of a dynamic receptor-based pharmacophore
model (DPM) of PfSpdSyn complemented by a knowledge-based rational
design strategy. The use of pharmacophore models to identify lead
compounds has become increasingly popular over the last decade and
has been shown to be a reliable method in the drug discovery
process. The development of a DPM allows for the incorporation of
protein exibility within the drug design process. This methodology
results in a wealth of information of the chemical space of the
active site and was incorporated in designing new inhibitors
against PfSpdSyn using a knowledge-based rational design strategy.
The active site of PfSpdSyn was subdivided into four binding
regions (DPM1-DPM4) to allow for the identi cation of fragments
binding within these speci c binding regions. DPMs representative
of the chemical characteristics of each binding region were
constructed and subsequently screened against the drug-like subset
of the ZINC database. From the screens a total of nine compounds
were selected for in vitro testing, complementing each other in
exploring specific active site binding characteristics. From these
compounds a new lead compound N-(3-aminopropyl)-cyclohexylamine
(NAC; Ki 2.8 μM) was identified for PfSpdSyn. NAC was specifically
designed to bind in both the putrescine and decarboxylated
adenosylmethionine cavities by chemically bridging the catalytic
center and was confirmed by kinetic studies. NAC shows great
potential for lead optimization to increase its binding affinity.
This study then paves the way for lead optimization and possibly
the development of a novel antimalarial. The development of a DPM
for PfSpdSyn has seen the establishment of this methodology in the
Bioinformatics and Computational Biology Unit, Department of
Biochemistry at the University of Pretoria. It can be concluded
that the development of a DPM complemented by a knowledge-based
rational design strategy is an effective approach for the…
Advisors/Committee Members: Prof A I Louw (advisor), Dr L M Birkholtz (advisor), Prof F Joubert (advisor), Prof J M Briggs (advisor).
Subjects/Keywords: Spermidine
synthase; Plasmodium
falciparum;
UCTD
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APA ·
Chicago ·
MLA ·
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CSE |
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APA (6th Edition):
Burger, P. B. (2009). Development of a
dynamic receptor-based pharmacophore model of Plasmodium falciparum
spermidine synthase for selective inhibitor
identification. (Doctoral Dissertation). University of Pretoria. Retrieved from http://hdl.handle.net/2263/24974
Chicago Manual of Style (16th Edition):
Burger, Pieter Buys. “Development of a
dynamic receptor-based pharmacophore model of Plasmodium falciparum
spermidine synthase for selective inhibitor
identification.” 2009. Doctoral Dissertation, University of Pretoria. Accessed February 22, 2020.
http://hdl.handle.net/2263/24974.
MLA Handbook (7th Edition):
Burger, Pieter Buys. “Development of a
dynamic receptor-based pharmacophore model of Plasmodium falciparum
spermidine synthase for selective inhibitor
identification.” 2009. Web. 22 Feb 2020.
Vancouver:
Burger PB. Development of a
dynamic receptor-based pharmacophore model of Plasmodium falciparum
spermidine synthase for selective inhibitor
identification. [Internet] [Doctoral dissertation]. University of Pretoria; 2009. [cited 2020 Feb 22].
Available from: http://hdl.handle.net/2263/24974.
Council of Science Editors:
Burger PB. Development of a
dynamic receptor-based pharmacophore model of Plasmodium falciparum
spermidine synthase for selective inhibitor
identification. [Doctoral Dissertation]. University of Pretoria; 2009. Available from: http://hdl.handle.net/2263/24974
9.
Majeau, Alicia Catherine.
INVESTIGATING DIFFERENTIAL ATTRACTIVENESS OF HUMAN SKIN MICROBIOTA TO ANOPHELES GAMBIAE AS A POTENTIAL COMPONENT OF BAITED SUGAR TRAPS CONTAINING ANTI-PLASMODIUM BACTERIA FOR BIOLOGICAL CONTROL.
Degree: 2014, Johns Hopkins University
URL: http://jhir.library.jhu.edu/handle/1774.2/37217
► Malaria is a pressing global health problem that is difficult to eradicate or even control because of its complex biology. Currently employed control mechanisms are…
(more)
▼ Malaria is a pressing global health problem that is difficult to eradicate or even control because of its complex biology. Currently employed control mechanisms are inefficient, and as a result of the need for alternative interventions, some research has focused on investigating the influence of the microbiota on mosquito vector competence. While some bacteria of the mosquito midgut have been shown to confer refractoriness to the
Plasmodium parasite as well as shorten the mosquito lifespan, we are still far from a real world application of a bacterium as a biological control mechanism for malaria. Spiked sugar feeding stations have been proposed as a mechanism of introducing the bacteria into local vector populations. This project aimed to investigate means of attracting mosquitoes to feed on the malaria parasite-blocking Enterobacter (Esp_Z) bacteria- spiked sugar, using human skin microbiota isolates as potential attractants. We also investigated the impact of
bacterial exposure on mosquito life span and fecundity as general fitness parameters. Minimal fitness costs were observed by sugar-feeding
Plasmodium-killing bacteria in the lab environment. No foot microbiome isolate was found to attract mosquitoes on its own, although one was found to exert mosquito repelling activity. However when this bacterial isolate was combined with an attractant that zoophilic mosquitoes respond to, an increase in attraction was observed. While much work is still needed, our results provide useful knowledge for the development of this type of biological control.
Advisors/Committee Members: Dimopoulos, George (advisor).
Subjects/Keywords: Plasmodium falciparum; olfaction; skin microbiota
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APA (6th Edition):
Majeau, A. C. (2014). INVESTIGATING DIFFERENTIAL ATTRACTIVENESS OF HUMAN SKIN MICROBIOTA TO ANOPHELES GAMBIAE AS A POTENTIAL COMPONENT OF BAITED SUGAR TRAPS CONTAINING ANTI-PLASMODIUM BACTERIA FOR BIOLOGICAL CONTROL. (Thesis). Johns Hopkins University. Retrieved from http://jhir.library.jhu.edu/handle/1774.2/37217
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Majeau, Alicia Catherine. “INVESTIGATING DIFFERENTIAL ATTRACTIVENESS OF HUMAN SKIN MICROBIOTA TO ANOPHELES GAMBIAE AS A POTENTIAL COMPONENT OF BAITED SUGAR TRAPS CONTAINING ANTI-PLASMODIUM BACTERIA FOR BIOLOGICAL CONTROL.” 2014. Thesis, Johns Hopkins University. Accessed February 22, 2020.
http://jhir.library.jhu.edu/handle/1774.2/37217.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Majeau, Alicia Catherine. “INVESTIGATING DIFFERENTIAL ATTRACTIVENESS OF HUMAN SKIN MICROBIOTA TO ANOPHELES GAMBIAE AS A POTENTIAL COMPONENT OF BAITED SUGAR TRAPS CONTAINING ANTI-PLASMODIUM BACTERIA FOR BIOLOGICAL CONTROL.” 2014. Web. 22 Feb 2020.
Vancouver:
Majeau AC. INVESTIGATING DIFFERENTIAL ATTRACTIVENESS OF HUMAN SKIN MICROBIOTA TO ANOPHELES GAMBIAE AS A POTENTIAL COMPONENT OF BAITED SUGAR TRAPS CONTAINING ANTI-PLASMODIUM BACTERIA FOR BIOLOGICAL CONTROL. [Internet] [Thesis]. Johns Hopkins University; 2014. [cited 2020 Feb 22].
Available from: http://jhir.library.jhu.edu/handle/1774.2/37217.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Majeau AC. INVESTIGATING DIFFERENTIAL ATTRACTIVENESS OF HUMAN SKIN MICROBIOTA TO ANOPHELES GAMBIAE AS A POTENTIAL COMPONENT OF BAITED SUGAR TRAPS CONTAINING ANTI-PLASMODIUM BACTERIA FOR BIOLOGICAL CONTROL. [Thesis]. Johns Hopkins University; 2014. Available from: http://jhir.library.jhu.edu/handle/1774.2/37217
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
10.
Sonia Devi Lourembam.
Protective immune response in plasmodium falciparum
malaria: a molecular immunoepidemiological study; -.
Degree: Biotechnology, 2011, Tezpur University
URL: http://shodhganga.inflibnet.ac.in/handle/10603/9014
► Malaria is a major health problem with the disease burden mostly borne by economically productive ages. Recent studies indicate the disease is becoming more widespread…
(more)
▼ Malaria is a major health problem with the disease
burden mostly borne by economically productive ages. Recent studies
indicate the disease is becoming more widespread in south east Asia
with 10 of the 11 countries endemic for malaria and with 1.2
billion people at risk of exposure to the disease. India reported
the highest malaria confirmed (1563344) and death (1133) cases in
2009 from this region (WHO). The north-eastern region of India
(population 28.5 million) is highly endemic for malaria and has
been declared as a high risk zone by National Anti Malaria Program.
Assam contributes to 64.7% of the malaria positive cases in the
north-eastern region of which P. falciparum accounts for 58-75% of
the cases and the remainder are due to Plasmodium vivax. Though
people living in endemic area develop immunity, which can be either
anti disease or anti infection immunity, the factors delineating
them are not clear. It is thus felt that an in depth understanding
of the immune response leading to anti infection or to anti disease
responses in population genetic studies and elucidation of parasite
diversity is required for success of malaria control programs. The
present study was designed against this perspective with the
following objectives: 1) To study the clone multiplicity of the
parasite genotypes existing in the study area 2) To elucidate the
protective humoral immune response. 3) To elucidate the protective
cell mediated immune response. The study was conducted at Guabari
village of Baksa district and Kondoli Tea Estate (KTE) of Nagaon
district which are mesoendemic for malaria. The two sites differ in
population demography and accessibility to health care. Plasmodium
falciparum diversity was assessed by typing polymorphic block 2
region of Merozoite Surface Protein 1(MSP-1) gene using primary as
well as nested PCR cycles. A high degree of polymorphism in
isolates from the two study sites was seen with 33 alleles of MSP-1
seen in Guabari village and 18 alleles at KTE.
References p.143-164, Abstract given chapter
wise
Advisors/Committee Members: Baruah, S.
Subjects/Keywords: Biology; Plasmodium falciparum; Malaria; Cytokines
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APA ·
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APA (6th Edition):
Lourembam, S. D. (2011). Protective immune response in plasmodium falciparum
malaria: a molecular immunoepidemiological study; -. (Thesis). Tezpur University. Retrieved from http://shodhganga.inflibnet.ac.in/handle/10603/9014
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Lourembam, Sonia Devi. “Protective immune response in plasmodium falciparum
malaria: a molecular immunoepidemiological study; -.” 2011. Thesis, Tezpur University. Accessed February 22, 2020.
http://shodhganga.inflibnet.ac.in/handle/10603/9014.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Lourembam, Sonia Devi. “Protective immune response in plasmodium falciparum
malaria: a molecular immunoepidemiological study; -.” 2011. Web. 22 Feb 2020.
Vancouver:
Lourembam SD. Protective immune response in plasmodium falciparum
malaria: a molecular immunoepidemiological study; -. [Internet] [Thesis]. Tezpur University; 2011. [cited 2020 Feb 22].
Available from: http://shodhganga.inflibnet.ac.in/handle/10603/9014.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Lourembam SD. Protective immune response in plasmodium falciparum
malaria: a molecular immunoepidemiological study; -. [Thesis]. Tezpur University; 2011. Available from: http://shodhganga.inflibnet.ac.in/handle/10603/9014
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
University of Notre Dame
11.
Allison Ann Penner Regier.
A Flexible Comparative Genomics Framework for Integrating
Heterogeneous Sequence Data</h1>.
Degree: PhD, Computer Science and Engineering, 2011, University of Notre Dame
URL: https://curate.nd.edu/show/0k225b01f48
► Genome sequencing technologies have revolutionized biology in the past two decades, yet data analysis has lagged behind data production. In this thesis, we present…
(more)
▼ Genome sequencing technologies have
revolutionized biology in the past two decades, yet data analysis
has lagged behind data production. In this thesis, we present a
framework for analyzing genomic data in more flexible ways than
previous techniques. First, the framework allows researchers to
design analyses that compare genomic samples directly instead of
relying on reference-relative variant calls, as most current tools
do. Second, we provide utilities to look at both assembly data and
resequencing data in the same analysis, where previous tools were
restricted to either looking at an assembly or at resequencing
data. Finally, our framework allows researchers to flexibly
incorporate alignments to arbitrarily many reference sequences into
their analysis. We describe
FlexReseq, the software implementation of this framework. FlexReseq
allows researchers to easily customize resequencing analyses using
a simple configuration file to define positions of interest. We
give results from applications of these tools such as genotyping
strains of
Plasmodium falciparum, finding diversity and divergence
between strains of Anopheles gambiae, detecting inversions based on
assembly and alignment information from A. gambiae, and exploring
resequencing analysis using alignments to multiple reference
sequences.
Advisors/Committee Members: Mihai Pop, Committee Member, Scott J. Emrich, Committee Chair, Frank Collins, Committee Member, Kevin Bowyer, Committee Member, Nora Besansky, Committee Member.
Subjects/Keywords: anopheles gambiae; plasmodium falciparum; bioinformatics
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APA ·
Chicago ·
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Export
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Manager
APA (6th Edition):
Regier, A. A. P. (2011). A Flexible Comparative Genomics Framework for Integrating
Heterogeneous Sequence Data</h1>. (Doctoral Dissertation). University of Notre Dame. Retrieved from https://curate.nd.edu/show/0k225b01f48
Chicago Manual of Style (16th Edition):
Regier, Allison Ann Penner. “A Flexible Comparative Genomics Framework for Integrating
Heterogeneous Sequence Data</h1>.” 2011. Doctoral Dissertation, University of Notre Dame. Accessed February 22, 2020.
https://curate.nd.edu/show/0k225b01f48.
MLA Handbook (7th Edition):
Regier, Allison Ann Penner. “A Flexible Comparative Genomics Framework for Integrating
Heterogeneous Sequence Data</h1>.” 2011. Web. 22 Feb 2020.
Vancouver:
Regier AAP. A Flexible Comparative Genomics Framework for Integrating
Heterogeneous Sequence Data</h1>. [Internet] [Doctoral dissertation]. University of Notre Dame; 2011. [cited 2020 Feb 22].
Available from: https://curate.nd.edu/show/0k225b01f48.
Council of Science Editors:
Regier AAP. A Flexible Comparative Genomics Framework for Integrating
Heterogeneous Sequence Data</h1>. [Doctoral Dissertation]. University of Notre Dame; 2011. Available from: https://curate.nd.edu/show/0k225b01f48
University of Pretoria
12.
Makgatho, Ephraim
Marema.
Flow cytometric
evaluation of riminophenazines as antimalarial agents.
Degree: Pharmacology, 2010, University of Pretoria
URL: http://hdl.handle.net/2263/28057
► The in vitro antimalarial activity of clofazimine and seven of its analogues, all TMP(tetramethyl-piperidyl group)-derivatives except 8669, against the R8-1 and pfUP-1 laboratory strains of…
(more)
▼ The in vitro antimalarial activity of clofazimine and
seven of its analogues, all TMP(tetramethyl-piperidyl
group)-derivatives except 8669, against the R8-1 and pfUP-1
laboratory strains of
Plasmodium falciparum was investigated using
a flow cytometric procedure. The flow cytometric method was
compared with microscopy and radiometry for efficiency in
quantitating the level of parasitemia in malaria cultures. The flow
cytometric method compared well, as determined by the 81and and
Altman measure of agreement, with both microscopy and radiometry
and was chosen for use in this study due to its speed, precision
and convenience (includes a fixing step that allows samples to be
evaluated at anyone time). The riminophenazine agents were found to
exhibit antimalarial action of varying degrees: B669, B4100, B4103,
B4112 and B4158 showed the best activity followed by B4121 and
B4169. Clofazimine did not exhibit any activity at concentrations
up to 2µg/ml in this system. Their effective concentrations in
vitro were comparable to that of standard antimalarial agents such
as chloroquine. The agents B4103 and B4112 exhibited additive
antimalarial activities when combined with chloroquine. The
inclusion of the TMP group and extent of halogenation of six of the
riminophenazines tested indicate that it is these structural
properties which are the major determinants of the antiplasmodial
activity. This is the first study to establish an antiplasmodial
activity of riminophenazines and further tests are necessary to
establish their antiparasitic mode of action and therapeutic
potential in animal models of experimental
chemotherapy.
Advisors/Committee Members: Prof C E Medlen (advisor), Prof R Anderson (advisor).
Subjects/Keywords: Plasmodium
falciparum;
Clofazimine;
Antimalarial;
UCTD
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APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Makgatho, E. (2010). Flow cytometric
evaluation of riminophenazines as antimalarial agents. (Masters Thesis). University of Pretoria. Retrieved from http://hdl.handle.net/2263/28057
Chicago Manual of Style (16th Edition):
Makgatho, Ephraim. “Flow cytometric
evaluation of riminophenazines as antimalarial agents.” 2010. Masters Thesis, University of Pretoria. Accessed February 22, 2020.
http://hdl.handle.net/2263/28057.
MLA Handbook (7th Edition):
Makgatho, Ephraim. “Flow cytometric
evaluation of riminophenazines as antimalarial agents.” 2010. Web. 22 Feb 2020.
Vancouver:
Makgatho E. Flow cytometric
evaluation of riminophenazines as antimalarial agents. [Internet] [Masters thesis]. University of Pretoria; 2010. [cited 2020 Feb 22].
Available from: http://hdl.handle.net/2263/28057.
Council of Science Editors:
Makgatho E. Flow cytometric
evaluation of riminophenazines as antimalarial agents. [Masters Thesis]. University of Pretoria; 2010. Available from: http://hdl.handle.net/2263/28057
13.
Santolalla Robles, Meddly Leslye.
Prevalencia de mutaciones en los genes PFDHFR y PFDHPS de Plasmodium falciparum en muestras de pacientes con malaria severa y/o complicada, del banco de muestras biológicas del NAMRU-6.
Degree: 2015, National University of San Marcos
URL: http://cybertesis.unmsm.edu.pe/handle/cybertesis/4620
;
http://cybertesis.unmsm.edu.pe/bitstream/cybertesis%2F4620/2/bitstream
;
http://cybertesis.unmsm.edu.pe/bitstream/cybertesis%2F4620/1/bitstream
► Introducción: Malaria representa una emergencia médica debido a la posible complicación y muerte del paciente cuando este no fue tratado apropiadamente. Malaria severa y/o complicada…
(more)
▼ Introducción: Malaria representa una emergencia médica debido a la posible complicación y muerte del paciente cuando este no fue tratado apropiadamente. Malaria severa y/o complicada (MSC) es causada casi exclusivamente por Plasmodium falciparum. Uno de los factores de riesgo asociado con MSC es el tratamiento inadecuado de los casos de malaria no complicada (MNC). Objetivos: Se genotipificó a los genes dihidrofolato reductasa (Pfdhfr) y dihidropteroato sintasa (Pfdhps) en muestras de 60 pacientes con MSC. La resistencia al tratamiento combinado sulfadoxina-pirimetamina (SP) es causado principalmente por mutaciones puntuales en esos genes Diseño de estudio: Los pacientes con MSC de este estudio fueron enrolados durante el brote de malaria de 1998, cuando SP era la primera línea de tratamiento. Materiales y métodos: Se usó el método de secuenciamiento de Sanger para la identificación de los polimorfismos en el gen Pfdhfr y los métodos PCR-RFLP y PCR alelo-específico para el gen Pfdhps. Resultados: Se encontró que el 84% de las muestras tenían el genotipo del parásito cuádruple mutante N51I/S108N/I164L/inserción repetición Bolivia, y el 16% restante el genotipo mutante simple S108N. Con respecto al gen Pfdhps, encontramos cuatro genotipos, siendo el triple mutante A437G/K540E/A581G el más frecuente (78%). Conclusiones: Observamos que las mutaciones I164L de Pfdhfr y K540E de Pfdhps en los casos de MSC fueron más del doble de frecuente comparado con los reportes publicados en casos de MNC en la misma área y periodo de estudio.
– Introduction: Malaria represents a medical emergency because it may rapidly progress to complication and death without prompt and appropriate treatment. Severe and/or complicated malaria (SCM) is almost exclusively caused by Plasmodium falciparum. One of the risk factors associated with SCM is an inappropriate treatment of the noncomplicated malaria (NCM). Objectives: We genotyped the dihydrofolate reductase (Pfdhfr) and dihydropteroate synthase (Pfdhps) genes from 60 SCM patients. Resistance to SP in P. falciparum is caused mainly by specific mutations at those genes. Study design: SCM patients of this study were enrolled during the malaria outbreak in 1998, when sulfadoxine/pyrimethamine (SP) was the first line of treatment. Material and methods: We used a Sanger sequencing approach for the identification of polymorphisms at Pfdhfr gene codons, and in the case of Pfdhps gene we used a PCRRFLP and PCR allele-specific methodology. Results: We found that 84% of samples harbored a quadruple mutant genotype N51I/S108N/I164L/insertion Bolivia repeat, and the left 16% of the sample contained an infection with a simple mutant genotype (S108N). Regarding the Pfdhps gene, we found four genotypes, the triple mutant genotype A437G/K540E/A581G was the more frequent (78%). Conclusions: We observed that the mutations I164L and K540E, known as highly predictor to SP resistance, in this group of patients with SCM were twice of frequency of the mutations from patients with NCM from published reports, also…
Advisors/Committee Members: William Cornejo Medina.
Subjects/Keywords: Mutación; Plasmodium falciparum; Malaria
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APA ·
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CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Santolalla Robles, M. L. (2015). Prevalencia de mutaciones en los genes PFDHFR y PFDHPS de Plasmodium falciparum en muestras de pacientes con malaria severa y/o complicada, del banco de muestras biológicas del NAMRU-6. (Thesis). National University of San Marcos. Retrieved from http://cybertesis.unmsm.edu.pe/handle/cybertesis/4620 ; http://cybertesis.unmsm.edu.pe/bitstream/cybertesis%2F4620/2/bitstream ; http://cybertesis.unmsm.edu.pe/bitstream/cybertesis%2F4620/1/bitstream
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Santolalla Robles, Meddly Leslye. “Prevalencia de mutaciones en los genes PFDHFR y PFDHPS de Plasmodium falciparum en muestras de pacientes con malaria severa y/o complicada, del banco de muestras biológicas del NAMRU-6.” 2015. Thesis, National University of San Marcos. Accessed February 22, 2020.
http://cybertesis.unmsm.edu.pe/handle/cybertesis/4620 ; http://cybertesis.unmsm.edu.pe/bitstream/cybertesis%2F4620/2/bitstream ; http://cybertesis.unmsm.edu.pe/bitstream/cybertesis%2F4620/1/bitstream.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Santolalla Robles, Meddly Leslye. “Prevalencia de mutaciones en los genes PFDHFR y PFDHPS de Plasmodium falciparum en muestras de pacientes con malaria severa y/o complicada, del banco de muestras biológicas del NAMRU-6.” 2015. Web. 22 Feb 2020.
Vancouver:
Santolalla Robles ML. Prevalencia de mutaciones en los genes PFDHFR y PFDHPS de Plasmodium falciparum en muestras de pacientes con malaria severa y/o complicada, del banco de muestras biológicas del NAMRU-6. [Internet] [Thesis]. National University of San Marcos; 2015. [cited 2020 Feb 22].
Available from: http://cybertesis.unmsm.edu.pe/handle/cybertesis/4620 ; http://cybertesis.unmsm.edu.pe/bitstream/cybertesis%2F4620/2/bitstream ; http://cybertesis.unmsm.edu.pe/bitstream/cybertesis%2F4620/1/bitstream.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Santolalla Robles ML. Prevalencia de mutaciones en los genes PFDHFR y PFDHPS de Plasmodium falciparum en muestras de pacientes con malaria severa y/o complicada, del banco de muestras biológicas del NAMRU-6. [Thesis]. National University of San Marcos; 2015. Available from: http://cybertesis.unmsm.edu.pe/handle/cybertesis/4620 ; http://cybertesis.unmsm.edu.pe/bitstream/cybertesis%2F4620/2/bitstream ; http://cybertesis.unmsm.edu.pe/bitstream/cybertesis%2F4620/1/bitstream
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
University of Pretoria
14.
Burger, Pieter Buys.
Development of a dynamic receptor-based pharmacophore
model of Plasmodium falciparum spermidine synthase for selective
inhibitor identification
.
Degree: 2009, University of Pretoria
URL: http://upetd.up.ac.za/thesis/available/etd-05252009-220942/
► Malaria affects the daily lives of more than 2 billion people worldwide and has been estimated to result in 300-500 million clinical cases annually leading…
(more)
▼ Malaria affects the daily lives of more than 2
billion people worldwide and has been estimated to result in
300-500 million clinical cases annually leading to approximately 2
million deaths, mainly caused by the most virulent malaria species,
Plasmodium falciparum. The lack of a vaccine and the rapid
emergence and spread of drug resistant strains of P.
falciparum,
necessitate the development of new antimalarials and the
identification and validation of new parasite-specific therapeutic
targets. Numerous studies directed at interfering with the
polyamine biosynthetic pathway in P.
falciparum have shown its
potential as a target for the development of a new class of
antimalarials. The essential nature of P.
falciparum spermidine
synthase (PfSpdSyn), an enzyme in the polyamine pathway of the
parasite warranted the further investigation to find novel lead
compounds. The high cost and attrition rate of drug discovery has
resulted in the implementation of smart drug discovery platforms in
both academia and industry. The strategy implemented in this study
involved the development of a dynamic receptor-based pharmacophore
model (DPM) of PfSpdSyn complemented by a knowledge-based rational
design strategy. The use of pharmacophore models to identify lead
compounds has become increasingly popular over the last decade and
has been shown to be a reliable method in the drug discovery
process. The development of a DPM allows for the incorporation of
protein exibility within the drug design process. This methodology
results in a wealth of information of the chemical space of the
active site and was incorporated in designing new inhibitors
against PfSpdSyn using a knowledge-based rational design strategy.
The active site of PfSpdSyn was subdivided into four binding
regions (DPM1-DPM4) to allow for the identi cation of fragments
binding within these speci c binding regions. DPMs representative
of the chemical characteristics of each binding region were
constructed and subsequently screened against the drug-like subset
of the ZINC database. From the screens a total of nine compounds
were selected for in vitro testing, complementing each other in
exploring specific active site binding characteristics. From these
compounds a new lead compound N-(3-aminopropyl)-cyclohexylamine
(NAC; Ki 2.8 μM) was identified for PfSpdSyn. NAC was specifically
designed to bind in both the putrescine and decarboxylated
adenosylmethionine cavities by chemically bridging the catalytic
center and was confirmed by kinetic studies. NAC shows great
potential for lead optimization to increase its binding affinity.
This study then paves the way for lead optimization and possibly
the development of a novel antimalarial. The development of a DPM
for PfSpdSyn has seen the establishment of this methodology in the
Bioinformatics and Computational Biology Unit, Department of
Biochemistry at the University of Pretoria. It can be concluded
that the development of a DPM complemented by a knowledge-based
rational design strategy is an effective approach for the…
Advisors/Committee Members: Prof A I Louw (advisor), Dr L M Birkholtz (advisor), Prof F Joubert (advisor), Prof J M Briggs (advisor).
Subjects/Keywords: Spermidine synthase;
Plasmodium falciparum;
UCTD
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APA (6th Edition):
Burger, P. B. (2009). Development of a dynamic receptor-based pharmacophore
model of Plasmodium falciparum spermidine synthase for selective
inhibitor identification
. (Doctoral Dissertation). University of Pretoria. Retrieved from http://upetd.up.ac.za/thesis/available/etd-05252009-220942/
Chicago Manual of Style (16th Edition):
Burger, Pieter Buys. “Development of a dynamic receptor-based pharmacophore
model of Plasmodium falciparum spermidine synthase for selective
inhibitor identification
.” 2009. Doctoral Dissertation, University of Pretoria. Accessed February 22, 2020.
http://upetd.up.ac.za/thesis/available/etd-05252009-220942/.
MLA Handbook (7th Edition):
Burger, Pieter Buys. “Development of a dynamic receptor-based pharmacophore
model of Plasmodium falciparum spermidine synthase for selective
inhibitor identification
.” 2009. Web. 22 Feb 2020.
Vancouver:
Burger PB. Development of a dynamic receptor-based pharmacophore
model of Plasmodium falciparum spermidine synthase for selective
inhibitor identification
. [Internet] [Doctoral dissertation]. University of Pretoria; 2009. [cited 2020 Feb 22].
Available from: http://upetd.up.ac.za/thesis/available/etd-05252009-220942/.
Council of Science Editors:
Burger PB. Development of a dynamic receptor-based pharmacophore
model of Plasmodium falciparum spermidine synthase for selective
inhibitor identification
. [Doctoral Dissertation]. University of Pretoria; 2009. Available from: http://upetd.up.ac.za/thesis/available/etd-05252009-220942/
University of Pretoria
15.
[No author].
Flow cytometric evaluation of riminophenazines as
antimalarial agents
.
Degree: 2010, University of Pretoria
URL: http://upetd.up.ac.za/thesis/available/etd-09202010-113318/
► The in vitro antimalarial activity of clofazimine and seven of its analogues, all TMP(tetramethyl-piperidyl group)-derivatives except 8669, against the R8-1 and pfUP-1 laboratory strains of…
(more)
▼ The in vitro antimalarial activity of clofazimine
and seven of its analogues, all TMP(tetramethyl-piperidyl
group)-derivatives except 8669, against the R8-1 and pfUP-1
laboratory strains of
Plasmodium falciparum was investigated using
a flow cytometric procedure. The flow cytometric method was
compared with microscopy and radiometry for efficiency in
quantitating the level of parasitemia in malaria cultures. The flow
cytometric method compared well, as determined by the 81and and
Altman measure of agreement, with both microscopy and radiometry
and was chosen for use in this study due to its speed, precision
and convenience (includes a fixing step that allows samples to be
evaluated at anyone time). The riminophenazine agents were found to
exhibit antimalarial action of varying degrees: B669, B4100, B4103,
B4112 and B4158 showed the best activity followed by B4121 and
B4169. Clofazimine did not exhibit any activity at concentrations
up to 2µg/ml in this system. Their effective concentrations in
vitro were comparable to that of standard antimalarial agents such
as chloroquine. The agents B4103 and B4112 exhibited additive
antimalarial activities when combined with chloroquine. The
inclusion of the TMP group and extent of halogenation of six of the
riminophenazines tested indicate that it is these structural
properties which are the major determinants of the antiplasmodial
activity. This is the first study to establish an antiplasmodial
activity of riminophenazines and further tests are necessary to
establish their antiparasitic mode of action and therapeutic
potential in animal models of experimental
chemotherapy.
Advisors/Committee Members: Prof C E Medlen (advisor), Prof R Anderson (advisor).
Subjects/Keywords: Plasmodium falciparum;
Clofazimine;
Antimalarial;
UCTD
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APA ·
Chicago ·
MLA ·
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CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
author], [. (2010). Flow cytometric evaluation of riminophenazines as
antimalarial agents
. (Masters Thesis). University of Pretoria. Retrieved from http://upetd.up.ac.za/thesis/available/etd-09202010-113318/
Chicago Manual of Style (16th Edition):
author], [No. “Flow cytometric evaluation of riminophenazines as
antimalarial agents
.” 2010. Masters Thesis, University of Pretoria. Accessed February 22, 2020.
http://upetd.up.ac.za/thesis/available/etd-09202010-113318/.
MLA Handbook (7th Edition):
author], [No. “Flow cytometric evaluation of riminophenazines as
antimalarial agents
.” 2010. Web. 22 Feb 2020.
Vancouver:
author] [. Flow cytometric evaluation of riminophenazines as
antimalarial agents
. [Internet] [Masters thesis]. University of Pretoria; 2010. [cited 2020 Feb 22].
Available from: http://upetd.up.ac.za/thesis/available/etd-09202010-113318/.
Council of Science Editors:
author] [. Flow cytometric evaluation of riminophenazines as
antimalarial agents
. [Masters Thesis]. University of Pretoria; 2010. Available from: http://upetd.up.ac.za/thesis/available/etd-09202010-113318/
16.
Mwenechanya, Roy.
In-vivo and Molecular characterization of drug resistant plasmodium falciparum isolates from Solwezi, Zambia.
Degree: 2011, University of Zimbabwe
URL: http://dspace.unza.zm/handle/123456789/283
► The study assessed the prevalence of drug resistant malarial parasites in patients at Solwezi urban clinic in northwestern province of Zambia in February 2004, using…
(more)
▼ The study assessed the prevalence of drug resistant malarial parasites in patients at
Solwezi urban clinic in northwestern province of Zambia in February 2004, using the
in-vivo and molecular methods. In the in-vivo method each patient was monitored for
signs and symptoms of the disease following treatment with either
sulfadoxine/pyrimethamine (fansidar®), lumefantrine/artemether (coartem®) or
quinine. Molecular determination of mutations on the Plasmodium falciparum
chloroquine resistance transporter gene (pfcrt), associated with chloroquine
resistance, and the dihydropteroate synthase gene (dhps) and the dihydrofolate
reductase gene (dhfr), associated with sulfadoxine and pyrimethamine resistance,
respectively, was also done on parasites obtained from the same patients, before
treatment, on the day of recruitment to the study. The extracted Plasmodium DNA
was amplified using nested PCR and the resulting secondary PCR products were
digested with restriction enzymes. The digested DNA fragments were separated by
electrophoresis using 2 - 2.5% agarose gels and visualized by using UV
transillumination after staining with ethidium bromide.
In-vivo analysis revealed a 27% (n=74) fansidar® treatment failure rate. The
molecular analysis revealed 11% (n=108) prevalence of the combination mutation at
codons 437 and 108 of the dhps and dhfr, respectively, associated with fansidar®
resistance. There was a significant association between the presence of a
combination of mutations on both genes and the in-vivo S/P treatment response in
patients (x , P = 0.001). There was, however, no significant association between the
in-vivo response in patients that received sulfadoxine/pyrimethamine treatment and
the presence of a single or combined mutation(s) on either the dhps (codon 437: x2,
P = 0.143; codon 540: x2, P = 0.205; codon 437/540: x2, P = 0.454) or the dhfr
(codon 108: x2, P = 0.390; codon 59: x2, P = 0.246; codons 108/59: x2, P = 0.969)
alone. Molecular analysis showed a 97% (152) prevalence of the pre-requisite
mutation K76T, for chloroquine resistance while the supporting mutation at codon 75
had a prevalence of 99% (n=155). The higher in-vivo treatment failure rate in
comparison with the lower prevalence of mutation associated with resistance
recorded could be attributed to other causes other than the presence of drug resistant
parasites.
The study demonstrated that the collection of P. falciparum infected blood samples
on filter papers and the use of restriction fragment length polymorphism molecular
analysis is a viable method that can be employed in the identification of drug
resistant Plasmodium isolates.
Subjects/Keywords: Plasmodium falciparum – Solwezi – Zambia
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APA ·
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Export
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APA (6th Edition):
Mwenechanya, R. (2011). In-vivo and Molecular characterization of drug resistant plasmodium falciparum isolates from Solwezi, Zambia. (Thesis). University of Zimbabwe. Retrieved from http://dspace.unza.zm/handle/123456789/283
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Mwenechanya, Roy. “In-vivo and Molecular characterization of drug resistant plasmodium falciparum isolates from Solwezi, Zambia.” 2011. Thesis, University of Zimbabwe. Accessed February 22, 2020.
http://dspace.unza.zm/handle/123456789/283.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Mwenechanya, Roy. “In-vivo and Molecular characterization of drug resistant plasmodium falciparum isolates from Solwezi, Zambia.” 2011. Web. 22 Feb 2020.
Vancouver:
Mwenechanya R. In-vivo and Molecular characterization of drug resistant plasmodium falciparum isolates from Solwezi, Zambia. [Internet] [Thesis]. University of Zimbabwe; 2011. [cited 2020 Feb 22].
Available from: http://dspace.unza.zm/handle/123456789/283.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Mwenechanya R. In-vivo and Molecular characterization of drug resistant plasmodium falciparum isolates from Solwezi, Zambia. [Thesis]. University of Zimbabwe; 2011. Available from: http://dspace.unza.zm/handle/123456789/283
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Harvard University
17.
Ravel, Deepali B.
Mechanisms of Cellular Remodeling during Plasmodium falciparum Sequestration and Transmission.
Degree: PhD, 2017, Harvard University
URL: http://nrs.harvard.edu/urn-3:HUL.InstRepos:42061497
► Despite significant progress in global malaria control, Plasmodium falciparum remains a major cause of morbidity and mortality world-wide. The need for new interventions demands better…
(more)
▼ Despite significant progress in global malaria control, Plasmodium falciparum remains a major cause of morbidity and mortality world-wide. The need for new interventions demands better understanding of the underlying cellular mechanisms that mediate parasite survival and transmission. Asexual growth and the development of sexual stages, termed gametocytes, take place in human red blood cells, and sexual reproduction takes place in the mosquito vector. Blood stage parasites sequester in host tissues to survive, requiring extensive cellular remodeling. Sequestering asexual stage parasites export hundreds of proteins into the host cell to change its deformability and cytoadherence. Developing gametocytes also sequester in deep tissues before reentering circulation to mediate transmission, and they too are characterized by protein export, deformability changes, and likely cytoadherence. Although significant progress has been made in understanding remodeling changes that occur to mediate sequestration and transmission, many open questions remain.
In this work, we identified and characterized proteins involved in cellular remodeling of asexual and sexual stage parasites. We first investigated red blood cell remodeling and sequestration during blood stage development, focusing specifically on the Plasmodium helical interspersed sub-telomeric c (PHISTc) protein family. Using a combination of biochemistry, biophysics, flow cytometry, and microscopy, we showed that PHISTc proteins are exported to the host cell of asexual and sexual stages and are required for the delivery of specific antigens to the surface of asexual stage infected red blood cells. Further, they play this specialized role in antigen delivery without drastically altering cellular architecture.
Next, we investigated the mechanisms that drive the transition of stiff, sequestered gametocytes into deformable, circulating gametocytes. Using biophysical and transcriptional approaches, we identified genes that are enriched or depleted in deformable gametocytes. Some of these candidate genes likely remodel the host red blood cell while others may remodel the parasite itself, with some candidates required for the formation of deformable, infectious gametocytes. We anticipate that these genes either drive or delineate the deformability transition and could represent possible targets of therapeutics or diagnostics used to reduce malaria transmission.
Together, these studies reveal both conserved and stage-specific mechanisms involved in the successful sequestration and transmission of Plasmodium falciparum.
Medical Sciences
Advisors/Committee Members: Wirth, Dyann (committee member), Dvorin, Jeffrey (committee member), Goldberg, Daniel (committee member).
Subjects/Keywords: Plasmodium falciparum; cellular remodeling
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APA ·
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CSE |
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to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Ravel, D. B. (2017). Mechanisms of Cellular Remodeling during Plasmodium falciparum Sequestration and Transmission. (Doctoral Dissertation). Harvard University. Retrieved from http://nrs.harvard.edu/urn-3:HUL.InstRepos:42061497
Chicago Manual of Style (16th Edition):
Ravel, Deepali B. “Mechanisms of Cellular Remodeling during Plasmodium falciparum Sequestration and Transmission.” 2017. Doctoral Dissertation, Harvard University. Accessed February 22, 2020.
http://nrs.harvard.edu/urn-3:HUL.InstRepos:42061497.
MLA Handbook (7th Edition):
Ravel, Deepali B. “Mechanisms of Cellular Remodeling during Plasmodium falciparum Sequestration and Transmission.” 2017. Web. 22 Feb 2020.
Vancouver:
Ravel DB. Mechanisms of Cellular Remodeling during Plasmodium falciparum Sequestration and Transmission. [Internet] [Doctoral dissertation]. Harvard University; 2017. [cited 2020 Feb 22].
Available from: http://nrs.harvard.edu/urn-3:HUL.InstRepos:42061497.
Council of Science Editors:
Ravel DB. Mechanisms of Cellular Remodeling during Plasmodium falciparum Sequestration and Transmission. [Doctoral Dissertation]. Harvard University; 2017. Available from: http://nrs.harvard.edu/urn-3:HUL.InstRepos:42061497
University of Ghana
18.
Mensah, B.A.
Assessment of Genetic Diversity, Complexity of Infection of Plasmodium Falciparum and Resistance to Antimalarial Drugs in Two Ecological Zones in Ghana
.
Degree: 2018, University of Ghana
URL: http://ugspace.ug.edu.gh/handle/123456789/32951
► Introduction: One major challenge to the global agenda for the elimination of malaria is the extensive genetic diversity of the parasite population, resulting in the…
(more)
▼ Introduction: One major challenge to the global agenda for the elimination of malaria is the extensive genetic diversity of the parasite population, resulting in the development of drug resistance and variation in antigens targeted for vaccine development. The aim of this study was to decipher any ecological difference in the evolution of drug resistance and to determine how much the increased use of artemisinin-based combination therapies (ACTs) and other control interventions are shaping the genetic diversity of the Plasmodium falciparum population in two ecologically distinct populations in Ghana.
Methods: A total of 803 dried blood spots (DBS) collected on filter paper from symptomatic children, aged 6 months to 14 years, with P. falciparum mono-infection in the coastal savanna (Cape-Coast) and the forest (Begoro) zones of Ghana from 2014 to 2017. In addition, a total of 991 P. falciparum infected DBS were collected from asymptomatic school children, aged 6 years to 14 years from 2013 to 2017. The study leveraged the high specificity and relatively low-cost of targeted next generation sequencing using molecular inversion probes for targeting and sequencing on the illumina MISEQ platform for sequencing of P. falciparum genes (pfcrt, pfdhfr, pfdhps, pfmdr1 and pfk13) implicated in anti-malarial resistance to chloroquine (CQ), sulfadoxine pyrimethamine (SP), lumefantrine, amodiaquine and artemisinin. The Plasmodium falciparum Apical Membrane Antigen 1 (pfama1) gene was also sequenced for the genetic diversity and complexity of P. falciparum infections (COI) analysis. Genetic diversity was compared between the two study populations and the sequences from Ghana were compared with sequences from West Africa, East Africa and South East Asia obtained from the gene bank.
Results: The result showed high genetic diversity in pfama1 in Ghanaian sequences with a total of 164 pfama1 haplotypes and a haplotype diversity of 0.993. There was no genetic differentiation between the two study populations in Ghana. Parasite isolates from the two ecological zones in Ghana showed a moderate genetic differentiation with sequences from Thailand (Fst=0.054) and low differentiation with sequences from Kenya (Fst=0.004). Seventy three percent (73%) of the infections were monoclonal. The major molecular marker associated with CQ resistance pfcrt K76T significantly reduced over the four years of the study (χ² = 40.57; p<0.001). The rate of re-expansion of chloroquine sensitive strains pfcrt K76 was higher in the forest ecological zone compared to the coastal savanna zone. The pfmdr1 184F mutant associated with lumefantrine resistance remained high over the years (68% to 83%). The prevalence of the quadruple mutation (IRNGK), associated with sulfadoxine-pyrimethamine resistance is almost at fixation, whilst pfdhps 540E has remained very low in Ghana. In addition, the study found low prevalence of pfdhps 581G mutation associated with sulfadoxine resistance, which has not been previously reported in these parts of Ghana. The South East Asian…
Subjects/Keywords: Plasmodium Falciparum;
Antimalarial Drugs;
Ghana
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APA ·
Chicago ·
MLA ·
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CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Mensah, B. A. (2018). Assessment of Genetic Diversity, Complexity of Infection of Plasmodium Falciparum and Resistance to Antimalarial Drugs in Two Ecological Zones in Ghana
. (Doctoral Dissertation). University of Ghana. Retrieved from http://ugspace.ug.edu.gh/handle/123456789/32951
Chicago Manual of Style (16th Edition):
Mensah, B A. “Assessment of Genetic Diversity, Complexity of Infection of Plasmodium Falciparum and Resistance to Antimalarial Drugs in Two Ecological Zones in Ghana
.” 2018. Doctoral Dissertation, University of Ghana. Accessed February 22, 2020.
http://ugspace.ug.edu.gh/handle/123456789/32951.
MLA Handbook (7th Edition):
Mensah, B A. “Assessment of Genetic Diversity, Complexity of Infection of Plasmodium Falciparum and Resistance to Antimalarial Drugs in Two Ecological Zones in Ghana
.” 2018. Web. 22 Feb 2020.
Vancouver:
Mensah BA. Assessment of Genetic Diversity, Complexity of Infection of Plasmodium Falciparum and Resistance to Antimalarial Drugs in Two Ecological Zones in Ghana
. [Internet] [Doctoral dissertation]. University of Ghana; 2018. [cited 2020 Feb 22].
Available from: http://ugspace.ug.edu.gh/handle/123456789/32951.
Council of Science Editors:
Mensah BA. Assessment of Genetic Diversity, Complexity of Infection of Plasmodium Falciparum and Resistance to Antimalarial Drugs in Two Ecological Zones in Ghana
. [Doctoral Dissertation]. University of Ghana; 2018. Available from: http://ugspace.ug.edu.gh/handle/123456789/32951
Université Laval
19.
Ebrahimzadeh, Zeinab.
Exploring the roles of phosphoinositides in the biology
of the malaria parasite Plasmodium falciparum.
Degree: 2019, Université Laval
URL: http://hdl.handle.net/20.500.11794/37075
► Plasmodium falciparum est un parasite appartenant au phylum Apicomplexa et est à l’origine de la forme la plus sévère de la malaria. Dans les zones…
(more)
▼ Plasmodium falciparum est un parasite appartenant
au phylum Apicomplexa et est à l’origine de la forme la plus sévère
de la malaria. Dans les zones endémiques d'Afrique subsaharienne,
la plupart des victimes sont des enfants de moins de cinq ans.
L’entrée de P.
falciparum dans sa cellule cible, le globule rouge,
repose sur la sécrétion de protéines par des organites spécialisés
: les micronèmes, les rhoptries et les granules denses. Les
mécanismes de biogenèse de ces organites et la coordination de la
libération de leur contenu lors de l'invasion sont cependant pour
la plupart inconnus. Il a été toutefois été démontré que les
protéines destinées à ces organites apicaux se concentrent dans des
microdomaines de l’appareil de Golgi, dont la composition en
lipides et en protéines détermine leur destination finale. À ce
jour, les mécanismes de sélection et de transport des protéines
apicales vers les organites d'invasion ainsi que leurs mécanismes
de sécrétion durant l’invasion sont pour la plupart inconnus. Nous
avons donc posé l’hypothèse que les phosphoinositides (PI) et leurs
protéines effectrices sont impliqués dans ces processus chez P.
falciparum. Les PI sont sept lipides phosphorylés retrouvés de
façon minoritaire dans les différentes membranes cellulaires.
Chaque membrane subcellulaire contient une espèce caractéristique
de PI qui peut être reconnue et liée spécifiquement par des
protéines effectrices. Une large gamme de processus biologiques
sont régulés par les PI, tels le trafic vésiculaire, les canaux
ioniques, les pompes d’efflux et les transporteurs, ainsi que
certains processus endocytiques et exocytaires. Des études
antérieures ont été en mesure de détecter seulement cinq des sept
espèces de PI chez P.
falciparum. Dans le cadre d’un premier
projet, nous avons étudié la distribution de six PI, à savoir PI3P,
PI4P, PI5P, PI (4,5)P2, PI(3,4)P2 et PI(3,4,5)P3, chez P.
falciparum. Pour ce faire, nous avons exprimé chez le parasite des
rapporteurs spécifiques correspondant à des domaines humains de
liaison aux PI, fusionnés à une protéine fluorescente. Cette
méthode nous a permis de confirmer des rapports antérieurs sur la
localisation du PI3P dans la membrane de la vacuole alimentaire,
dans de petites vésicules près ou sur la membrane plasmique du
parasite ainsi qu’à l’apicoplaste. De plus, nous avons révélé pour
la première fois la présence de PI5P chez P.
falciparum et montré
qu’il se localisait à la membrane plasmique, au noyau et
potentiellement dans le réticulum endoplasmique de transition. Nous
avons aussi montré que le PI4P est localisé dans la membrane
plasmique ainsi que dans l’appareil de Golgi et que le PI(4,5)P2
est présent dans la membrane plasmique tout au long du cycle
érythrocytaire. Cette carte de la distribution subcellulaire des PI
constitue un excellent outil pour mieux déchiffrer les rôles de ces
lipides chez le parasite P.
falciparum. Dans le cadre d’un second
projet, nous avons caractérisé une protéine possédant un domaine
conservé chez les Apicomplexa, le domain d’homologie de la…
Advisors/Committee Members: Richard, Dave.
Subjects/Keywords: Phospho-inositides; Plasmodium falciparum
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APA ·
Chicago ·
MLA ·
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CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Ebrahimzadeh, Z. (2019). Exploring the roles of phosphoinositides in the biology
of the malaria parasite Plasmodium falciparum. (Thesis). Université Laval. Retrieved from http://hdl.handle.net/20.500.11794/37075
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Ebrahimzadeh, Zeinab. “Exploring the roles of phosphoinositides in the biology
of the malaria parasite Plasmodium falciparum.” 2019. Thesis, Université Laval. Accessed February 22, 2020.
http://hdl.handle.net/20.500.11794/37075.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Ebrahimzadeh, Zeinab. “Exploring the roles of phosphoinositides in the biology
of the malaria parasite Plasmodium falciparum.” 2019. Web. 22 Feb 2020.
Vancouver:
Ebrahimzadeh Z. Exploring the roles of phosphoinositides in the biology
of the malaria parasite Plasmodium falciparum. [Internet] [Thesis]. Université Laval; 2019. [cited 2020 Feb 22].
Available from: http://hdl.handle.net/20.500.11794/37075.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Ebrahimzadeh Z. Exploring the roles of phosphoinositides in the biology
of the malaria parasite Plasmodium falciparum. [Thesis]. Université Laval; 2019. Available from: http://hdl.handle.net/20.500.11794/37075
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
20.
Dennis, Adelaide Sandra Mild.
PfATP4 and the biochemical signature of PfATP4-associated compounds
.
Degree: 2018, Australian National University
URL: http://hdl.handle.net/1885/149540
► In 2016 more than 200 million cases of malaria were reported and nearly 500 000 people died from the disease. Although there are antimalarial drugs…
(more)
▼ In 2016 more than 200 million cases of malaria were reported and
nearly 500 000 people died from the disease. Although there are
antimalarial drugs available, the most virulent species of the
disease-causing parasite, Plasmodium falciparum, has evolved some
level of resistance to many of them. A global effort to
accelerate antimalarial drug discovery has led to the finding
that one particular parasite protein, the P-type ATPase PfATP4,
appears to be the target of a number of potent novel antimalarial
compounds. Two of these ‘PfATP4-associated compounds’ have
entered the clinical pipeline; one of these is the spiroindolone
KAE609 (also known as cipargamin).
PfATP4 is located on the plasma membrane of the parasite.
Although an early study yielded evidence consistent with PfATP4
being a Ca2+ transporter, more recent studies have provided
evidence that PfATP4 extrudes Na+ ions from the parasite while
importing H+ ions, allowing the parasite to maintain a large
inward Na+ concentration gradient. When parasites are exposed to
PfATP4-associated compounds, there is an increase in the Na+
concentration and the pH inside the parasite.
In the work reported in this Thesis I have investigated the
nature and function of PfATP4, extended the characterisation of
the effects of PfATP4-associated compounds on mature
asexual-stage P. falciparum parasites, and identified a number of
new compounds of this class.
A phylogenetic analysis revealed that PfATP4 belongs to a unique
subgroup of Type II P-type ATPases, specific to apicomplexan
parasites and their closest relatives.
Previous studies have reported that PfATP4-associated compounds
cause parasite swelling. The effect of KAE609 on the volume of
isolated parasites and parasitised erythrocytes was characterised
using a Coulter Multisizer. KAE609 caused isolated parasites to
swell in a Na+-dependent manner. KAE609 also caused intact
infected erythrocytes to swell and thereby increase in osmotic
fragility. Another six PfATP4-associated compounds were also
shown to induce parasite swelling. Protecting parasitised
erythrocytes from excessive swelling by growing them in a
hyperosmotic medium increased the concentration of KAE609 that
was required to kill the parasite, consistent with cell swelling
playing a role in the mechanism of action of KAE609.
The ‘biochemical signature’ of PfATP4-associated compounds
was used to identify novel PfATP4-associated compounds from a set
of 400 diverse drug-like compounds. Experiments investigating the
effects of these compounds on parasite Na+, pH and volume, as
well as cross-resistance studies using KAE609-resistant
parasites, provided evidence that eleven compounds act in a
manner consistent with inhibition of PfATP4. These compounds add
to the chemical diversity of known…
Subjects/Keywords: PfATP4;
Plasmodium falciparum;
malaria;
spiroindolone
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APA (6th Edition):
Dennis, A. S. M. (2018). PfATP4 and the biochemical signature of PfATP4-associated compounds
. (Thesis). Australian National University. Retrieved from http://hdl.handle.net/1885/149540
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Dennis, Adelaide Sandra Mild. “PfATP4 and the biochemical signature of PfATP4-associated compounds
.” 2018. Thesis, Australian National University. Accessed February 22, 2020.
http://hdl.handle.net/1885/149540.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Dennis, Adelaide Sandra Mild. “PfATP4 and the biochemical signature of PfATP4-associated compounds
.” 2018. Web. 22 Feb 2020.
Vancouver:
Dennis ASM. PfATP4 and the biochemical signature of PfATP4-associated compounds
. [Internet] [Thesis]. Australian National University; 2018. [cited 2020 Feb 22].
Available from: http://hdl.handle.net/1885/149540.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Dennis ASM. PfATP4 and the biochemical signature of PfATP4-associated compounds
. [Thesis]. Australian National University; 2018. Available from: http://hdl.handle.net/1885/149540
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Universidade Estadual de Campinas
21.
Naranjo Prado, Isabel Cristina, 1987-.
Avaliação ex-vivo dos efeitos antimaláricos da violaceína em isolados amazônicos de Plasmodium vivax e P. falciparum e análise da sua atividade em camundongos infectados com cepas de P. chabaudi resistentes a antimaláricos
.
Degree: 2014, Universidade Estadual de Campinas
URL: http://repositorio.unicamp.br/jspui/handle/REPOSIP/316728
► Resumo: A malária é responsável por cerca de 300 milhões de casos de infecção e 1 milhão de mortes por ano. No Brasil, em 2012,…
(more)
▼ Resumo: A malária é responsável por cerca de 300 milhões de casos de infecção e 1 milhão de mortes por ano. No Brasil, em 2012, foram registrados cerca de 1500 casos sendo a malária vivax responsável por 85% destes. Ainda, é frequentemente reportada falha terapêutica aos antimaláricos convencionais (como cloroquina e mefloquina) em infecções por P.
falciparum, principalmente, mas também por P. vivax. Sendo assim, terapias combinadas com artemisinina e seus derivados (ACT) são atualmente recomendadas. No entanto, resistência aos derivados da artemisinina já é evidente e a busca por novos compostos com atividade antimalárica é urgente. A violaceína, pigmento violeta extraído de bactérias Gram negativas, demostrou apresentar elevada atividade antimalárica in vitro e in vivo em trabalho anterior de nosso grupo. Neste sentido, este projeto tem como objetivo aprofundar a investigação a respeito da atividade antimalárica da violaceína avaliando seu papel em isolados amazônicos de P.
falciparum adaptados recentemente em cultura, e em P. vivax após imediata coleta de sangue de pacientes infectados. Além disso, investigamos o potencial da violaceína como terapia combinada junto ao artesunato no tratamento de parasitas murinos resistentes a este e a outros antimaláricos como a cloroquina. Inicialmente foram testadas dois diferentes tipos de violaceína in vitro contra P.
falciparum 3D7: uma comercial extraída de Janthinobacterium lividum (vJl- IC50: 227 nM) e outra extraída de Chromobacterium violaceum (vCv- IC50: 390 nM). Apesar de não termos encontrado uma diferença na atividade antimalárica entre as duas violaceínas, a extraída de C. violaceum teve uma baixa toxicidade em eritrócitos (<400 nM) em células de hepatoma humano (<800 nM). Devido a esta baixa toxicidade, somente vCv foi avaliada quanto sua atividade antimalárica. Demonstramos que vCv apresentou IC50 similar ao encontrado para P.
falciparum 3D7 (IC50 média= 419,8 nM) em 7 isolados de campo de P.
falciparum. Ainda, em ensaios in vivo, utilizando cepas murinas de P. chabaudi, vCv conseguiu diminuir significativamente (P<0,05) a parasitemia no dia pico da infeção em cepas resistentes à cloroquina (30CQ) e a artesunato e mefloquina (ATNMF1). Adicionalmente, nos testes realizados em P. vivax a vCv parece evitar o amadurecimento parasitário nos quatro isolados testados. Coletivamente, podemos concluir que a vCv apresentou um efeito antimalárico em cepas de P.
falciparum e pode ser especialmente útil quando usada em combinação com artesunato no tratamento de camundongos infectados com cepas resistentes. Finalmente, ensaios adicionais de amadurecimento com isolados de P. vivax necessitam ser conduzidos para a comprovação do efeito da vCv nesta espécie; Abstract: Malaria is responsible for about 300 million infections and one million deaths per year. In Brazil in 2012, about 1500 cases were reported and malaria vivax accounts for 85% of these. Still, it is frequently reported treatment failure with conventional antimalarials (as chloroquine and mefloquine) mainly in…
Advisors/Committee Members: Costa, Fabio Trindade Maranhão, 1972- (advisor), Lopes, Stefanie Costa Pinto, 1983- (advisor), Brocchi, Marcelo (committee member), Cravo, Pedro Vitor Lemos (committee member).
Subjects/Keywords: Violaceina;
Plasmodium chabaudi;
Plasmodium falciparum;
Artemisinina
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APA ·
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APA (6th Edition):
Naranjo Prado, Isabel Cristina, 1. (2014). Avaliação ex-vivo dos efeitos antimaláricos da violaceína em isolados amazônicos de Plasmodium vivax e P. falciparum e análise da sua atividade em camundongos infectados com cepas de P. chabaudi resistentes a antimaláricos
. (Thesis). Universidade Estadual de Campinas. Retrieved from http://repositorio.unicamp.br/jspui/handle/REPOSIP/316728
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Naranjo Prado, Isabel Cristina, 1987-. “Avaliação ex-vivo dos efeitos antimaláricos da violaceína em isolados amazônicos de Plasmodium vivax e P. falciparum e análise da sua atividade em camundongos infectados com cepas de P. chabaudi resistentes a antimaláricos
.” 2014. Thesis, Universidade Estadual de Campinas. Accessed February 22, 2020.
http://repositorio.unicamp.br/jspui/handle/REPOSIP/316728.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Naranjo Prado, Isabel Cristina, 1987-. “Avaliação ex-vivo dos efeitos antimaláricos da violaceína em isolados amazônicos de Plasmodium vivax e P. falciparum e análise da sua atividade em camundongos infectados com cepas de P. chabaudi resistentes a antimaláricos
.” 2014. Web. 22 Feb 2020.
Vancouver:
Naranjo Prado, Isabel Cristina 1. Avaliação ex-vivo dos efeitos antimaláricos da violaceína em isolados amazônicos de Plasmodium vivax e P. falciparum e análise da sua atividade em camundongos infectados com cepas de P. chabaudi resistentes a antimaláricos
. [Internet] [Thesis]. Universidade Estadual de Campinas; 2014. [cited 2020 Feb 22].
Available from: http://repositorio.unicamp.br/jspui/handle/REPOSIP/316728.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Naranjo Prado, Isabel Cristina 1. Avaliação ex-vivo dos efeitos antimaláricos da violaceína em isolados amazônicos de Plasmodium vivax e P. falciparum e análise da sua atividade em camundongos infectados com cepas de P. chabaudi resistentes a antimaláricos
. [Thesis]. Universidade Estadual de Campinas; 2014. Available from: http://repositorio.unicamp.br/jspui/handle/REPOSIP/316728
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Addis Ababa University
22.
Ahmed, Muzemil.
Determination of Artemisinin and essential oil contents of Artemisia annua L. grown in Ethiopia and In vivo Antimalarial activity of its crude extracts against Plasmodium berghei in mice
.
Degree: 2009, Addis Ababa University
URL: http://etd.aau.edu.et/dspace/handle/123456789/3662
► Abstract Malaria is a major public health problem in the world in general and developing country in particular. It causes about 1.5-3 millions deaths per…
(more)
▼ Abstract
Malaria is a major public health problem in the world in general and developing
country in particular. It causes about 1.5-3 millions deaths per year, an annual
incidence of 300-500 million clinical cases. Chloroquine has been the drug of
choice for chemoprophylaxis and treatment of P.
falciparum for several decades, but
its clinical utility greatly reduced in many malaria endemic regions due to the spread
of chloroquine resistance. Chinese investigators introduced a new compound,
artemisinin, a sesquiterpene lactone, from Artemisia annua a few years back. It
possesses a potent antimalarial activity with different mechanism of action from that
of the conventional drugs in use. Currently the plant is introduced to many African
countries including Ethiopia. Reports at different countries showed the variability of
artemisinin content for the same plant grown at different geographical regions.
Seventy percent ethanol and hot water crude extracts from the dried leaves of A.
annua grown at Wondogenet (Ethiopia) gave 20.2 and 14.00 % yields per dry weight
respectively. Preliminary phytochemical screening using standard procedures
showed the presence of steroidal compounds, phenolics, flavonoids and trace
amount of tannins. Quantification of artemisinin from leaf of crude ethanol extract of
A. annua was carried out using HPLC-UV with an attempt to show the plant is
biosynthesizing artemisinin. The limiting step in sample preparation was the
presence of large amounts of chlorophyll in the crude extract. The standard
calibration curve for the quantification was linear with correlation coefficient of
0.96150. In this study the average content of artemisinin (w/w) was determined to be
low in concentration (0.014% ± 0.001). Analysis of essential oil (0.32%) from A.
annua by GC/MS gave a total of 38 compounds comprised of 99.72 % of the total
volatile constituents and of these twenty-two monoterpenes (57.89%), fourteen
sesquiterpens (36.84%) and two phenols (5.55%) were identified. Among the volatile
constituents camphor was identified to be the major component (43.84%).
An in vivo experiment was performed to know weather A. annua grown in Ethiopia
has retained its antimalarial activity. The two crude extracts at three different doses
viii
were tested on mice infected with
Plasmodium berghei, in a four day test procedure.
The results of the in vivo experiment showed that the parasite multiplication was
inhibited by 77.97 % and 64.75 % for 70 % ethanol and hot water extracts
respectively at dose level of 450 mg/kg. The study revealed that A. annua grown in
Ethiopia retained the desired antiplasmodial activity, as seen from its suppressive
effects against P. berghei.
Advisors/Committee Members: Dr. Ariaya Hymete,Dr. Mekuria Tadesse,Ato Abebe Animute (advisor).
Subjects/Keywords: Malaria; Plasmodium falciparum; Plasmodium berghei; Artemisia annua
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APA ·
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Export
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APA (6th Edition):
Ahmed, M. (2009). Determination of Artemisinin and essential oil contents of Artemisia annua L. grown in Ethiopia and In vivo Antimalarial activity of its crude extracts against Plasmodium berghei in mice
. (Thesis). Addis Ababa University. Retrieved from http://etd.aau.edu.et/dspace/handle/123456789/3662
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Ahmed, Muzemil. “Determination of Artemisinin and essential oil contents of Artemisia annua L. grown in Ethiopia and In vivo Antimalarial activity of its crude extracts against Plasmodium berghei in mice
.” 2009. Thesis, Addis Ababa University. Accessed February 22, 2020.
http://etd.aau.edu.et/dspace/handle/123456789/3662.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Ahmed, Muzemil. “Determination of Artemisinin and essential oil contents of Artemisia annua L. grown in Ethiopia and In vivo Antimalarial activity of its crude extracts against Plasmodium berghei in mice
.” 2009. Web. 22 Feb 2020.
Vancouver:
Ahmed M. Determination of Artemisinin and essential oil contents of Artemisia annua L. grown in Ethiopia and In vivo Antimalarial activity of its crude extracts against Plasmodium berghei in mice
. [Internet] [Thesis]. Addis Ababa University; 2009. [cited 2020 Feb 22].
Available from: http://etd.aau.edu.et/dspace/handle/123456789/3662.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Ahmed M. Determination of Artemisinin and essential oil contents of Artemisia annua L. grown in Ethiopia and In vivo Antimalarial activity of its crude extracts against Plasmodium berghei in mice
. [Thesis]. Addis Ababa University; 2009. Available from: http://etd.aau.edu.et/dspace/handle/123456789/3662
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
23.
Parraguez de la Cruz, Jorge Enrique.
Antígenos relevantes de Plasmodium vivax y Plasmodium falciparum detectados mediante inmunoblot : Iquitos 2004.
Degree: 2008, National University of San Marcos
URL: http://cybertesis.unmsm.edu.pe/handle/cybertesis/448
;
http://cybertesis.unmsm.edu.pe/bitstream/cybertesis%2F448/1/bitstream
► El objetivo del presente trabajo fue identificar antígenos relevantes de valor diagnóstico de aislados de P. vivax y P. falciparum provenientes del departamento de Loreto,…
(more)
▼ El objetivo del presente trabajo fue identificar antígenos relevantes de valor diagnóstico de aislados de P. vivax y P.
falciparum provenientes del departamento de Loreto, mediante la técnica de inmunoblot. Se seleccionaron pacientes entre 3 y 64 años con diagnóstico de malaria, gota gruesa positiva, procedentes de centros de salud en el departamento de Loreto. Fueron analizadas 4 mezclas de antígenos, una de P.
falciparum (PF1) y tres de P. vivax (PV1, PV4 y PV5), preparadas a partir de 36 muestras de pacientes con alta parasitemia por P. vivax (2 700 – 69 000 parásitos/μL) y P.
falciparum (2 750 – 10 000 parásitos/μL). Las mezclas de antígenos fueron enfrentadas a 39 sueros (12 de P.
falciparum y 27 de P. vivax) mediante ensayos de inmunoblot.
Advisors/Committee Members: Cornejo Medina, William Renee.
Subjects/Keywords: Malaria - Diagnóstico; Plasmodium falciparum; Plasmodium vivax
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APA (6th Edition):
Parraguez de la Cruz, J. E. (2008). Antígenos relevantes de Plasmodium vivax y Plasmodium falciparum detectados mediante inmunoblot : Iquitos 2004. (Thesis). National University of San Marcos. Retrieved from http://cybertesis.unmsm.edu.pe/handle/cybertesis/448 ; http://cybertesis.unmsm.edu.pe/bitstream/cybertesis%2F448/1/bitstream
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Parraguez de la Cruz, Jorge Enrique. “Antígenos relevantes de Plasmodium vivax y Plasmodium falciparum detectados mediante inmunoblot : Iquitos 2004.” 2008. Thesis, National University of San Marcos. Accessed February 22, 2020.
http://cybertesis.unmsm.edu.pe/handle/cybertesis/448 ; http://cybertesis.unmsm.edu.pe/bitstream/cybertesis%2F448/1/bitstream.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Parraguez de la Cruz, Jorge Enrique. “Antígenos relevantes de Plasmodium vivax y Plasmodium falciparum detectados mediante inmunoblot : Iquitos 2004.” 2008. Web. 22 Feb 2020.
Vancouver:
Parraguez de la Cruz JE. Antígenos relevantes de Plasmodium vivax y Plasmodium falciparum detectados mediante inmunoblot : Iquitos 2004. [Internet] [Thesis]. National University of San Marcos; 2008. [cited 2020 Feb 22].
Available from: http://cybertesis.unmsm.edu.pe/handle/cybertesis/448 ; http://cybertesis.unmsm.edu.pe/bitstream/cybertesis%2F448/1/bitstream.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Parraguez de la Cruz JE. Antígenos relevantes de Plasmodium vivax y Plasmodium falciparum detectados mediante inmunoblot : Iquitos 2004. [Thesis]. National University of San Marcos; 2008. Available from: http://cybertesis.unmsm.edu.pe/handle/cybertesis/448 ; http://cybertesis.unmsm.edu.pe/bitstream/cybertesis%2F448/1/bitstream
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
24.
Sethia, Sonal.
Etudes métabolomiques du métabolisme du carbone des stades érythrocytaires asexués du parasite du paludisme humain Plasmodium falciparum. : Use of metabolomics to decipher parasite carbon metabolism of asexual erythrocytic stages of the human malaria parasite Plasmodium falciparum.
Degree: Docteur es, Biologie Santé, 2015, Montpellier
URL: http://www.theses.fr/2015MONTT046
► Le paludisme est une des maladies tropicales les plus dévastatrices au monde causée par des parasites protozoaires intracellulaires du genre Plasmodium. Cinq espèces de plasmodies…
(more)
▼ Le paludisme est une des maladies tropicales les plus dévastatrices au monde causée par des parasites protozoaires intracellulaires du genre
Plasmodium. Cinq espèces de plasmodies sont responsables du paludisme chez l'homme et causent 600 000 décès par an principalement chez les enfants de moins de 5 ans et les femmes enceintes vivant dans les régions les plus pauvres du globe. Les parasites ont généré une résistance contre les chimiothérapies existantes et aucun vaccin efficace n'est encore disponible. Il est donc impératif d'identifier et de valider de nouvelles cibles qui peuvent être exploitées pour la découverte de nouveaux médicaments.Cette étude a porté sur la caractérisation d'un enzyme, la phosphoénolpyruvate carboxylase (PEPC), produit d'un gène spécifique au parasite et absent chez l'hôte humain, ce qui constitue l'un des pré-requis d'une cible potentielle pour la découverte de médicaments. Le gène avait été montré comme essentiel pour des parasites seulement en absence de malate ou de fumarate, suggérant un rôle de la protéine dans le métabolisme du carbone intermédiaire des parasites.Mes études de thèse avaient pour but de caractériser le rôle de la PEPC en utilisant la métabolomique. J'ai d'abord établi et normalisé une méthodologie d'analyses métabolomiques des globules rouges infectés par
Plasmodium et optimisé l'analyse des métabolites hydrophiles présents dans le parasite intracellulaire et sa cellule hôte. Nous nous sommes concentrés sur les métabolites du métabolisme du carbone intermédiaire, où la PEPC pouvait jouer un rôle déterminant par analogie avec les plantes et les bactéries. Des analyses ciblées utilisant un marquage isotopique du métabolome à partir de 13C-U-glucose, 13C-bicarbonate et 13C, 15N-glutamine ont aussi été réalisées permettant de mieux appréhender les conséquences d'un KO de l'enzyme PEPC sur le métabolisme du parasite.Les données montrent que l'enzyme PEPC permet une fixation du bicarbonate et catalyse une réaction anaplérotique conduisant à du malate qui est introduit dans le cycle de l'acide tricarboxylique mitochondrial, transférant ainsi des équivalents réducteurs du cytoplasme à la mitochondrie et fournissant aussi un point d'entrée du squelette carboné dans le cycle. Les résultats montrent surtout que les parasites possèdent un cycle complet et de type oxydatif de l'acide tricarboxylique mitochondrial. Il parait y avoir trois points d'entrée: 1. l'acétyl CoA résultant du pyruvate généré par la glycolyse et décarboxylé dans la mitochondrie; 2. l'acide alpha-cétoglutarique provenant du glutamate, qui lui-même résulte de la désamination de la glutamine essentiellement fournie par l'environnement externe; 3. le malate, produit en aval de la malate déshydrogénase qui réduit l'oxaloacétate produit par la PEPC. En aval de la PEPC, la biosynthèse des pyrimidines opère grâce à l'activité de l'aspartate aminotransférase agissant sur oxaloacétate.En dehors du malate, le fumarate est le seul autre métabolite qui permet de s'opposer au défaut de croissance des parasites déficients…
Advisors/Committee Members: Müller, Sylke (thesis director), Vial, Henri (thesis director).
Subjects/Keywords: Plasmodium Falciparum; Métabolomique; Métabolisme du carbone; Plasmodium Falciparum; Metabolomics; Carbon metabolism
Record Details
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APA ·
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Export
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APA (6th Edition):
Sethia, S. (2015). Etudes métabolomiques du métabolisme du carbone des stades érythrocytaires asexués du parasite du paludisme humain Plasmodium falciparum. : Use of metabolomics to decipher parasite carbon metabolism of asexual erythrocytic stages of the human malaria parasite Plasmodium falciparum. (Doctoral Dissertation). Montpellier. Retrieved from http://www.theses.fr/2015MONTT046
Chicago Manual of Style (16th Edition):
Sethia, Sonal. “Etudes métabolomiques du métabolisme du carbone des stades érythrocytaires asexués du parasite du paludisme humain Plasmodium falciparum. : Use of metabolomics to decipher parasite carbon metabolism of asexual erythrocytic stages of the human malaria parasite Plasmodium falciparum.” 2015. Doctoral Dissertation, Montpellier. Accessed February 22, 2020.
http://www.theses.fr/2015MONTT046.
MLA Handbook (7th Edition):
Sethia, Sonal. “Etudes métabolomiques du métabolisme du carbone des stades érythrocytaires asexués du parasite du paludisme humain Plasmodium falciparum. : Use of metabolomics to decipher parasite carbon metabolism of asexual erythrocytic stages of the human malaria parasite Plasmodium falciparum.” 2015. Web. 22 Feb 2020.
Vancouver:
Sethia S. Etudes métabolomiques du métabolisme du carbone des stades érythrocytaires asexués du parasite du paludisme humain Plasmodium falciparum. : Use of metabolomics to decipher parasite carbon metabolism of asexual erythrocytic stages of the human malaria parasite Plasmodium falciparum. [Internet] [Doctoral dissertation]. Montpellier; 2015. [cited 2020 Feb 22].
Available from: http://www.theses.fr/2015MONTT046.
Council of Science Editors:
Sethia S. Etudes métabolomiques du métabolisme du carbone des stades érythrocytaires asexués du parasite du paludisme humain Plasmodium falciparum. : Use of metabolomics to decipher parasite carbon metabolism of asexual erythrocytic stages of the human malaria parasite Plasmodium falciparum. [Doctoral Dissertation]. Montpellier; 2015. Available from: http://www.theses.fr/2015MONTT046
Université Montpellier II
25.
Pulcini, Serena.
Studies on the mechanisms of action of artemisinins and the role of PfATP6 : Les études sur les mécanismes d'action de l'artémisinine et le rôle des PfATP6.
Degree: Docteur es, Interface chimie-Biologie : systèmes moléculaires à visée thérapeutique, 2011, Université Montpellier II
URL: http://www.theses.fr/2011MON20138
► La pompe ATPase Ca2+ du réticulum sarco-endoplasmique Plasmodium falciparum (PfATP6) est une protéine de dix transmembranes, impliqué dans la régulation de l'homéostasie du calcium dans…
(more)
▼ La pompe ATPase Ca2+ du réticulum sarco-endoplasmique
Plasmodium falciparum (PfATP6) est une protéine de dix transmembranes, impliqué dans la régulation de l'homéostasie du calcium dans le parasite. L'importance d'étudier cette protéine repose sur l'hypothèse d'être engagé dans le mécanisme d'action et de résistance des artémisinines. Des travaux précédents, fondé sur l'expression hétérologue dans des ovocytes de Xenopus laevis et Saccharomyces cerevisiae, ont montré des résultats opposés, générant de nombreux corollaires vérifiables. Par conséquent, des travaux supplémentaires sont nécessaires pour mieux comprendre la nature des interactions entre les artémisinines et transporteurs de type SERCA.Afin d'évaluer le caractère essentiel du gène de
Plasmodium spp., une approche de génétique inverse a été utilisée. Knockout du gène, soit P.
falciparum et berghei, ne pouvant pas être obtenu. La complémentation de sauvetage épisomique a été jugée impossible. Marquage à la fin 3' de PfATP6 et PbATP6 a été, également, tenté pour étudier la localisation et l'expression de la protéine chez les parasites. La manipulation des gènes à cette place n'a pas permis la survie du parasite. Nos résultats, pris ensemble, montrent que ATP6 est essentiel dans
Plasmodium spp..Au cours de nos études génétiques, un phénotype stable et particulier de parasites du genre
Plasmodium falciparum 3D7 a été distingué. Les étranges parasites “monstres" contiennent une vacuole digestive inhabituelle gonflées à travers toutes les étapes du développement du parasite. Caractérisation de l'insolite
Plasmodium a été réalisée, montrant une sensibilité accrue à la chloroquine, mais pas à l'artémisinine ou de la méfloquine. Tenant compte de la similitude du PfATP6 avec la pompe SERCA orthologue mammifère, de nouvelles molécules, connu et synthétisé pour cibler spécifiquement la protéine chez les mammifères, ont été testés sur P.
falciparum. Quatre classes différentes de composés (sHA 14-1, BHQ, chalcone et des analogues de l'ACP) a montré le blocage de la croissance in vitro du P.
falciparum 3D7 et Dd2 à des concentrations inférieure au range micromolaire. En outre, une nouvelle classe de molécules (thaperoxides), conçu comme un hybride entre l'artémisinine et thapsigargine, a été testé contre le type sauvage 7G8 et la ligne muté L263E. Ce dernier porte une mutation ponctuelle unique de nucléotides dans PfATP6, déjà connu d'être impliqué dans la résistance du l'artémisinine.Compte tenu de la difficulté à manipuler les gènes du parasite, et afin de mieux caractériser PfATP6, un gène synthétique a été optimisé pour l'expression hétérologue chez S. cerevisiae. De cette façon, la complémentation d'une ligne de levure mutée (K616) sans les pompes endogènes Ca2+ de type P a été permis avec succès, montrant le sauvetage de la croissance de la levure en présence de forte concentration de calcium libre. Différents inhibiteurs de SERCA, comme la thapsigargine et l'acide cyclopiazonique, ont été testés sur la levure complémenté K616 PfATP6, afin de vérifier l'inhibition…
Advisors/Committee Members: Krishna, Sanjeev (thesis director).
Subjects/Keywords: Paludisme; Plasmodium falciparum; Artémisinines; PfATP6; Malaria; Plasmodium falciparum; Artemisinins; PfATP6
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APA (6th Edition):
Pulcini, S. (2011). Studies on the mechanisms of action of artemisinins and the role of PfATP6 : Les études sur les mécanismes d'action de l'artémisinine et le rôle des PfATP6. (Doctoral Dissertation). Université Montpellier II. Retrieved from http://www.theses.fr/2011MON20138
Chicago Manual of Style (16th Edition):
Pulcini, Serena. “Studies on the mechanisms of action of artemisinins and the role of PfATP6 : Les études sur les mécanismes d'action de l'artémisinine et le rôle des PfATP6.” 2011. Doctoral Dissertation, Université Montpellier II. Accessed February 22, 2020.
http://www.theses.fr/2011MON20138.
MLA Handbook (7th Edition):
Pulcini, Serena. “Studies on the mechanisms of action of artemisinins and the role of PfATP6 : Les études sur les mécanismes d'action de l'artémisinine et le rôle des PfATP6.” 2011. Web. 22 Feb 2020.
Vancouver:
Pulcini S. Studies on the mechanisms of action of artemisinins and the role of PfATP6 : Les études sur les mécanismes d'action de l'artémisinine et le rôle des PfATP6. [Internet] [Doctoral dissertation]. Université Montpellier II; 2011. [cited 2020 Feb 22].
Available from: http://www.theses.fr/2011MON20138.
Council of Science Editors:
Pulcini S. Studies on the mechanisms of action of artemisinins and the role of PfATP6 : Les études sur les mécanismes d'action de l'artémisinine et le rôle des PfATP6. [Doctoral Dissertation]. Université Montpellier II; 2011. Available from: http://www.theses.fr/2011MON20138
University of Ghana
26.
Quansah, E.B.
Functional Characterization of Plasmodium Falciparum Claudin-Like Apicomplexan Microneme Protein (Pfclamp) (Pf3d7_1030200)
.
Degree: 2019, University of Ghana
URL: http://ugspace.ug.edu.gh/handle/123456789/34631
► Malaria is still a public health burden. With the recent reports of artemisinin resistance in Plasmodium falciparum coupled with the low efficacy of the RTS,…
(more)
▼ Malaria is still a public health burden. With the recent reports of artemisinin resistance in Plasmodium falciparum coupled with the low efficacy of the RTS, S vaccine currently under a WHO-recommended pilot malaria vaccine implementation programme (MVIP), there is the need to continue identifying new targets by functionally characterizing some of the ~60% of the parasite’s genes with unknown functions. This will foster the identification of viable vaccine and possible drug targets for the development of interventions against the parasite. This project focused on studying P. falciparum Claudin-Like Apicomplexan Microneme Protein (PfCLAMP) with gene ID (Pf3D7_1030200) and its role during invasion of the parasite. CLAMP has been shown to be highly conserved in apicomplexans, with its orthologue in P. falciparum being essential for parasite growth and invasion. Here, using specific antibodies raised against the extracellular domain of the PfCLAMP, we report that PfCLAMP localizes at the apical portion of merozoites. The generated antibodies inhibited parasite invasion in a dose dependent manner. We have also observed that PfCLAMP is differentially expressed across the different asexual stages of the parasite, with peak expression occurring at the late stages of the parasite. We also discovered that some clinical isolates habour multiple copies of the PfCLAMP gene. Additionally, we show that PfCLAMP conditional gene knockout reduced invasion by up to 30% in the first cycle of the parasite development. Altogether, our data demonstrates that PfCLAMP provides a potentially attractive target for further investigation for drug and vaccine development.
Subjects/Keywords: Malaria;
Plasmodium Falciparum;
Ligand-Receptor;
Erythrocyte Invasion;
Plasmodium Falciparum
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APA (6th Edition):
Quansah, E. B. (2019). Functional Characterization of Plasmodium Falciparum Claudin-Like Apicomplexan Microneme Protein (Pfclamp) (Pf3d7_1030200)
. (Masters Thesis). University of Ghana. Retrieved from http://ugspace.ug.edu.gh/handle/123456789/34631
Chicago Manual of Style (16th Edition):
Quansah, E B. “Functional Characterization of Plasmodium Falciparum Claudin-Like Apicomplexan Microneme Protein (Pfclamp) (Pf3d7_1030200)
.” 2019. Masters Thesis, University of Ghana. Accessed February 22, 2020.
http://ugspace.ug.edu.gh/handle/123456789/34631.
MLA Handbook (7th Edition):
Quansah, E B. “Functional Characterization of Plasmodium Falciparum Claudin-Like Apicomplexan Microneme Protein (Pfclamp) (Pf3d7_1030200)
.” 2019. Web. 22 Feb 2020.
Vancouver:
Quansah EB. Functional Characterization of Plasmodium Falciparum Claudin-Like Apicomplexan Microneme Protein (Pfclamp) (Pf3d7_1030200)
. [Internet] [Masters thesis]. University of Ghana; 2019. [cited 2020 Feb 22].
Available from: http://ugspace.ug.edu.gh/handle/123456789/34631.
Council of Science Editors:
Quansah EB. Functional Characterization of Plasmodium Falciparum Claudin-Like Apicomplexan Microneme Protein (Pfclamp) (Pf3d7_1030200)
. [Masters Thesis]. University of Ghana; 2019. Available from: http://ugspace.ug.edu.gh/handle/123456789/34631
Rhodes University
27.
Goble, Jessica Leigh.
The druggable antimalarial target 1-deoxy-D-xylulose-5-phosphate reductoisomerase: purfication, kinetic characterization and inhibition studies.
Degree: Faculty of Science, Biochemistry, Microbiology and Biotechnology, 2011, Rhodes University
URL: http://hdl.handle.net/10962/d1004008
► Plasmodium falciparum 1–deoxy–D–xylulose–5 phosphatereductoisomerase (PfDXR) plays a role in isoprenoid biosynthesis in the malaria parasite and is absent in the human host, making this parasite…
(more)
▼ Plasmodium falciparum 1–deoxy–D–xylulose–5 phosphatereductoisomerase (PfDXR) plays a role in isoprenoid biosynthesis in the malaria parasite and is absent in the human host, making this parasite enzyme an attractive target for antimalarial drug design. To characterize PfDXR, it is necessary to produce large quantities of the enzyme in a soluble and functional form. However, the over–production of malarial proteins in prokaryotic host systems often results in the formation of truncated proteins or insoluble protein aggregates. A heterologous expression system was developed for the production of active PfDXR using codon harmonization and tight control of expression in the presence of lac repressor. Yields of up to 2 mg/l of enzyme were reported using the optimised expression system, which is 8 to 10– fold greater than previously reported yields. The kinetic parameters Km, Vmax and kcat were determined for PfDXR; values reported in this study were consistent with those reported in the literature for other DXR enzymes. A three–dimensional model of the malarial drug target protein PfDXR was generated, and validated using structure–checking programs and protein docking studies. Structural and functional features unique to PfDXR were identified using the model and comparative sequence analyses with apicomplexan and non–apicomplexan DXR proteins. Residues Val44 and Asn45, essential for NADPH binding; and catalytic hatch residues Lys224 and Lys226, which are unique to the species of Plasmodium, were mutated to resemble those of E. coli DXR. Interestingly,these mutations resulted in significant reductions in substrate affinity, when compared to the unmutated PfDXR. Mutant enzymes PfDXR(VN43,44AG) and PfDXR(KK224,226NS) also demonstrated a decreased ability to turnover substrate by 4–fold and 2–fold respectively. This study indicates a difference in the role of the catalytic hatch of PfDXR with regards to the way in which it captures substrates. The study also highlights subtle differences in cofactor binding to PfDXR, compared with the well characterized EcDXR enzyme. The validated PfDXR model was also used to develop a novel efficient in silico screening method for potential tool compounds for use in the rational design of novel DXR inhibitors. Following in silico screening of 46 potential DXR inhibitors, a two–tiered in vitro screening approach was undertaken. DXR inhibition was assessed for the 46 novel compounds using an NADPH– ependant DXP enzyme inhibition assay and antimalarial potential was assessed using P.falciparum–infected erythrocyte growth assays. Select compounds were tested in human cells in order to determine whether they were toxic to the host. From the parallel in silico and in vitro drug screening, it was evident that only a single compound demonstrated reasonable potential binding to DXR (determined using in silico docking), inhibited DXR in vitro and inhibited P. falciparum growth, without being toxic to human cells. Its potential as a lead compound in antimalarial drug development is therefore feasible.…
Subjects/Keywords: Antimalarials – Development; Plasmodium falciparum; Drug development; Plasmodium falciparum – Purification; Plasmodium falciparum – Inhibitors; Enzyme kinetics; Malaria – Chemotherapy
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APA ·
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APA (6th Edition):
Goble, J. L. (2011). The druggable antimalarial target 1-deoxy-D-xylulose-5-phosphate reductoisomerase: purfication, kinetic characterization and inhibition studies. (Thesis). Rhodes University. Retrieved from http://hdl.handle.net/10962/d1004008
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Goble, Jessica Leigh. “The druggable antimalarial target 1-deoxy-D-xylulose-5-phosphate reductoisomerase: purfication, kinetic characterization and inhibition studies.” 2011. Thesis, Rhodes University. Accessed February 22, 2020.
http://hdl.handle.net/10962/d1004008.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Goble, Jessica Leigh. “The druggable antimalarial target 1-deoxy-D-xylulose-5-phosphate reductoisomerase: purfication, kinetic characterization and inhibition studies.” 2011. Web. 22 Feb 2020.
Vancouver:
Goble JL. The druggable antimalarial target 1-deoxy-D-xylulose-5-phosphate reductoisomerase: purfication, kinetic characterization and inhibition studies. [Internet] [Thesis]. Rhodes University; 2011. [cited 2020 Feb 22].
Available from: http://hdl.handle.net/10962/d1004008.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Goble JL. The druggable antimalarial target 1-deoxy-D-xylulose-5-phosphate reductoisomerase: purfication, kinetic characterization and inhibition studies. [Thesis]. Rhodes University; 2011. Available from: http://hdl.handle.net/10962/d1004008
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Universidad del Rosario
28.
Patiño Molano, Liliana Catherine.
Caracterización de la proteína del cuello de las roptrias 5 en plasmodium falciparum (PfRON5).
Degree: 2011, Universidad del Rosario
URL: http://repository.urosario.edu.co/handle/10336/2678
► El conocimiento de las proteínas implicadas en el proceso de invasión de los merozoitos a los eritrocitos por Plasmodium es el punto de partida para…
(more)
▼ El conocimiento de las proteínas implicadas en el proceso de invasión de los merozoitos a los eritrocitos por Plasmodium es el punto de partida para el desarrollo de nuevas estrategias para controlar la malaria. Muchas de estas proteínas han sido estudiadas en Toxoplasma gondii, donde se han identificado las proteínas que pertenecen al Tight Junction (TJ), el cual permite una interacción fuerte entre las membranas de la célula huésped y el parásito, necesaria para la invasión parasitaria. En este género, cuatro proteínas del cuello de las roptrias (RON2, RON4, RON5 y RON8) y una proteína de micronemas (TgAMA-1) se han encontrado como parte del TJ. En Plasmodium falciparum, se han caracterizado las proteínas PfRON2 y PfRON4. En el presente estudio se realiza la identificación de la proteína PfRON5, una proteína de ~110 kDa que se expresa en las etapas de merozoitos y esquizontes de la cepa FCB-2 utilizando técnicas de biología molecular, bioinformática e inmuoquímica.
Gathering knowledge about the proteins involved in erythrocyte invasion by Plasmodium merozoites is the starting point for developing new strategies to control malarial disease. Many of these proteins have been studied in Toxoplasma gondii, where some belonging to the Moving Junction complex have been identified. This complex allows a strong interaction between host cell and parasite membranes, required for parasite invasion. In this genus, four rhoptry proteins (RON2, RON4, RON5 and RON8) and one micronemal protein (TgAMA-1) have been found as part of the complex. In Plasmodium falciparum, RON2 and RON4 have been characterized. In the present study, we identify PfRON5, a ~110 kDa protein which is expressed in merozoite and schizont stages of the FCB-2 strain using techniques of molecular biology, bioinformatics and inmuochemistry.
Fundación Instituto de Inmunología de Colombia
Advisors/Committee Members: Curtidor, Hernando.
Subjects/Keywords: Malaria; Homología; Merozoito; Plasmodium falciparum; MALARIA - INVESTIGACIONES; EPIDEMIOLOGIA - INVESTIGACIONES; Malaria; Homology; Merozoite; Plasmodium falciparum; PLASMODIUM FALCIPARUM - INVESTIGACIONES
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APA ·
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Export
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APA (6th Edition):
Patiño Molano, L. C. (2011). Caracterización de la proteína del cuello de las roptrias 5 en plasmodium falciparum (PfRON5). (Thesis). Universidad del Rosario. Retrieved from http://repository.urosario.edu.co/handle/10336/2678
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Patiño Molano, Liliana Catherine. “Caracterización de la proteína del cuello de las roptrias 5 en plasmodium falciparum (PfRON5).” 2011. Thesis, Universidad del Rosario. Accessed February 22, 2020.
http://repository.urosario.edu.co/handle/10336/2678.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Patiño Molano, Liliana Catherine. “Caracterización de la proteína del cuello de las roptrias 5 en plasmodium falciparum (PfRON5).” 2011. Web. 22 Feb 2020.
Vancouver:
Patiño Molano LC. Caracterización de la proteína del cuello de las roptrias 5 en plasmodium falciparum (PfRON5). [Internet] [Thesis]. Universidad del Rosario; 2011. [cited 2020 Feb 22].
Available from: http://repository.urosario.edu.co/handle/10336/2678.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Patiño Molano LC. Caracterización de la proteína del cuello de las roptrias 5 en plasmodium falciparum (PfRON5). [Thesis]. Universidad del Rosario; 2011. Available from: http://repository.urosario.edu.co/handle/10336/2678
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
29.
Martha Cecilia Suárez-Mutis.
Epidemiologia da malária em comunidades do rio Padauiri, médio rio Negro, uma área de extrativismo vegetal da piaçaba no estado de Amazonas,Brasil.
Degree: 2007, Fundação Oswaldo Cruz
URL: http://www.bdtd.cict.fiocruz.br/tedesimplificado/tde_busca/arquivo.php?codArquivo=104
► A região do rio Padauiri, no médio rio Negro, Estado do Amazonas, é uma área de extração de piaçaba com alta incidência de malária cuja…
(more)
▼ A região do rio Padauiri, no médio rio Negro, Estado do Amazonas, é uma área de extração de piaçaba com alta incidência de malária cuja epidemiologia local ainda não tinha sido estudada. Com o objetivo de avaliar a morbidade da malária nessa área foi desenhado um estudo observacional misto. Para conhecer a situação de base da malária e como não existia informação sistemática anterior foi realizado inicialmente um estudo retrospectivo, posteriormente um estudo piloto seccional para avaliar os antecedentes de malária, a partir do qual foi estabelecida uma coorte aberta durante 18 meses para acompanhar as pessoas que adquiriram infecção pelo Plasmódium e quem tinha feito quadro clínico de malária. As pessoas foram entrevistadas, submetidas a um exame físico direcionado aos sinais da malária e coletado sangue para realização de gota espessa, esfregaço de sangue periférico, PCR para diagnóstico molecular do parasita e sorologia para determinação de níveis de transmissão. Concomitantemente foram feitos estudos entomológicos procurando conhecer os principais vetores e seu comportamento assim como os prováveis criadouros naturais dos mesmos. Foi definido como caso de malária, toda pessoa com sintomas ou sinais da doença e com presença de plasmódios na gota espessa e como infecção assintomática toda pessoa com gota espessa positiva e/ou PCR positivo para qualquer uma das espécies do
Plasmodium que não tivesse tido sintomas em até 30 dias antes e depois da coleta da amostra. Para classificar o caso como assintomático a pessoa não podia ter tomado medicamentos antimaláricos nos últimos 30 dias antes da coleta da amostra. Foram acompanhadas 188 pessoas. Durante o período de acompanhamento não houve óbitos nem internações devidas à malária na coorte estudada. No total, 53,2% das pessoas (100/188) tiveram malária com 169 episódios diferentes da doença. A incidência parasitária anual na área foi de 602,3 para cada 1000 habitantes. Houve uma média de 1,7 episódios de malária por pessoa sendo as crianças com menos de 5 anos aquelas que tiveram maior risco para contrair a doença quando comparadas com o resto da população (RR:1,64 IC95%:1,26-2,14, p=0,012); a média de episódios de malária nessas crianças foi de 2,2. Apesar da ocorrência dos dois tipos de
Plasmodium mais comuns nas Américas, nos últimos anos há um predomínio de infecção pelo P. vivax na área, mostrando um padrão de mudança quando comparados com a década anterior. Foram encontrados casos de infecção assintomática por
Plasmodium (tanto P. vivax quanto P.
falciparum) em um percentual que flutuou entre 8,2 a 24%. Os episódios clínicos de malária foram sazonais e houve uma tendência a ocorrer no inicio do período das chuvas e no final das mesmas. A infecção assintomática, ao contrario foi mais freqüente durante o período da estação da seca. O principal transmissor encontrado foi o An. darlingi que apesar de ter sido coletado em todas as localidades, sua freqüência foi muito maior nas comunidades mais afastadas da desembocadura do rio Padauiri no rio Negro, nas áreas próximas…
Advisors/Committee Members: José Rodrigues Coura.
Subjects/Keywords: Malária; Comunidades Pequenas; Plasmodium falciparum; Plasmodium vivax; DOENCAS INFECCIOSAS E PARASITARIAS; Malaria; Small Communities; Plasmodium falciparum; Plasmodium vivax
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APA ·
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MLA ·
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CSE |
Export
to Zotero / EndNote / Reference
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APA (6th Edition):
Suárez-Mutis, M. C. (2007). Epidemiologia da malária em comunidades do rio Padauiri, médio rio Negro, uma área de extrativismo vegetal da piaçaba no estado de Amazonas,Brasil. (Thesis). Fundação Oswaldo Cruz. Retrieved from http://www.bdtd.cict.fiocruz.br/tedesimplificado/tde_busca/arquivo.php?codArquivo=104
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Suárez-Mutis, Martha Cecilia. “Epidemiologia da malária em comunidades do rio Padauiri, médio rio Negro, uma área de extrativismo vegetal da piaçaba no estado de Amazonas,Brasil.” 2007. Thesis, Fundação Oswaldo Cruz. Accessed February 22, 2020.
http://www.bdtd.cict.fiocruz.br/tedesimplificado/tde_busca/arquivo.php?codArquivo=104.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Suárez-Mutis, Martha Cecilia. “Epidemiologia da malária em comunidades do rio Padauiri, médio rio Negro, uma área de extrativismo vegetal da piaçaba no estado de Amazonas,Brasil.” 2007. Web. 22 Feb 2020.
Vancouver:
Suárez-Mutis MC. Epidemiologia da malária em comunidades do rio Padauiri, médio rio Negro, uma área de extrativismo vegetal da piaçaba no estado de Amazonas,Brasil. [Internet] [Thesis]. Fundação Oswaldo Cruz; 2007. [cited 2020 Feb 22].
Available from: http://www.bdtd.cict.fiocruz.br/tedesimplificado/tde_busca/arquivo.php?codArquivo=104.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Suárez-Mutis MC. Epidemiologia da malária em comunidades do rio Padauiri, médio rio Negro, uma área de extrativismo vegetal da piaçaba no estado de Amazonas,Brasil. [Thesis]. Fundação Oswaldo Cruz; 2007. Available from: http://www.bdtd.cict.fiocruz.br/tedesimplificado/tde_busca/arquivo.php?codArquivo=104
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
30.
Gaillard, Tiphaine.
Identification et validation de marqueurs moléculaires de la résistance de Plasmodium falciparum à la doxycycline : Identification and validation of molecular markers of resistance of plasmodium falciparum to doxycycline.
Degree: Docteur es, Pathologie humaine. Maladies infectieuses, 2015, Aix Marseille Université
URL: http://www.theses.fr/2015AIXM5036
► La doxycycline est l’une des molécules recommandées par l’OMS en prophylaxie pour les voyageurs dans les zones d’endémie palustre, en particulier dans les zones de…
(more)
▼ La doxycycline est l’une des molécules recommandées par l’OMS en prophylaxie pour les voyageurs dans les zones d’endémie palustre, en particulier dans les zones de multirésistance. Une étude récente avait suggéré que les isolats de P. falciparum présentaient différents niveaux de sensibilité à la doxycycline et que l’augmentation du nombre de copies de deux gènes, pfmdt ou pftetQ, pouvait être associée à une baisse de sensibilité.Le premier objectif de ce travail a consisté à valider ce modèle à partir d’un nouvel échantillonnage d’isolats africains. Le second objectif était d’évaluer le nombre de copies de ces deux gènes sur des isolats originaires de Thaïlande. Le troisième objectif a consisté à rechercher d’autres sources de résistance en investiguant le polymorphisme des gènes codant l’ARN ribosomal plasmodial potentiellement impliqués dans la résistance in vitro à la doxycycline.Les résultats nous ont permis de confirmer que le nombre de copies des gènes pfmdt ou pftetQ pouvait être impliqué dans la résistance in vitro à la doxycycline en Afrique. Les résultats concernant les isolats Thaï n’ont pas permis de corréler le nombre de copies des gènes pfmdt et pftetQ au phénotype CI50. Ces éléments montrent que ce mécanisme de résistance seul est insuffisant pour expliquer la résistance à la doxycycline ; les résultats sont en faveur d’une résistance médiée par plusieurs gènes.La recherche de points de mutation sur le gène pfssrRNA codant pour la petite sous-unité ribosomale de l’ADN plasmodial n’a pas abouti. D’autres cibles moléculaires sont en cours d’étude pour expliquer les mécanismes de résistance de P. falciparum à la doxycycline.
Doxycycline is currently one of the recommended chemoprophylactic regimens for travellers visiting malaria-endemic, particularly in countries with a high prevalence of resistance to chloroquine and multiresistance. A previous study suggested that increased pfmdt or pftetQ copy number could be associated with a lower susceptibility to doxycycline.The first aim of this study was to validate the pre-established model involving these two molecular markers with other African isolates. The second was to evaluate these markers in P. falciparum isolates coming from a multiresistance area in Thaïland. The third was to investigate the eventual association between the polymorphism in genes encoding ribosomal rRNA and in vitro resistance to doxycycline.The results confirm that pfmdt or pftetQ copy numbers should be involved in in vitro susceptibility to doxycycline in African P. falciparum isolates. The results concerning the Thai isolates indicate that there is no correlation between the pfmdt and pftetQ genes copy numbers and the belonging to the high doxycycline IC50 phenotype; this implies that this mechanism of resistance is not enough by itself to explain resistance to doxycycline; it augurs that the resistance to doxycycline should be controlled by multiple genes, and that these genetic markers could be continent-dependent. The search for points of mutation in isolates from the…
Advisors/Committee Members: Pradines, Bruno (thesis director).
Subjects/Keywords: Plasmodium; Plasmodium falciparum; Marqueurs moléculaires; Doxycycline; Tétracyclines; Macrolides; Antibiotiques; Plasmodium; Plasmodium falciparum; Molecular markers; Doxycycline; Tetracyclines; Macrolides; Antibiotics
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APA (6th Edition):
Gaillard, T. (2015). Identification et validation de marqueurs moléculaires de la résistance de Plasmodium falciparum à la doxycycline : Identification and validation of molecular markers of resistance of plasmodium falciparum to doxycycline. (Doctoral Dissertation). Aix Marseille Université. Retrieved from http://www.theses.fr/2015AIXM5036
Chicago Manual of Style (16th Edition):
Gaillard, Tiphaine. “Identification et validation de marqueurs moléculaires de la résistance de Plasmodium falciparum à la doxycycline : Identification and validation of molecular markers of resistance of plasmodium falciparum to doxycycline.” 2015. Doctoral Dissertation, Aix Marseille Université. Accessed February 22, 2020.
http://www.theses.fr/2015AIXM5036.
MLA Handbook (7th Edition):
Gaillard, Tiphaine. “Identification et validation de marqueurs moléculaires de la résistance de Plasmodium falciparum à la doxycycline : Identification and validation of molecular markers of resistance of plasmodium falciparum to doxycycline.” 2015. Web. 22 Feb 2020.
Vancouver:
Gaillard T. Identification et validation de marqueurs moléculaires de la résistance de Plasmodium falciparum à la doxycycline : Identification and validation of molecular markers of resistance of plasmodium falciparum to doxycycline. [Internet] [Doctoral dissertation]. Aix Marseille Université 2015. [cited 2020 Feb 22].
Available from: http://www.theses.fr/2015AIXM5036.
Council of Science Editors:
Gaillard T. Identification et validation de marqueurs moléculaires de la résistance de Plasmodium falciparum à la doxycycline : Identification and validation of molecular markers of resistance of plasmodium falciparum to doxycycline. [Doctoral Dissertation]. Aix Marseille Université 2015. Available from: http://www.theses.fr/2015AIXM5036
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