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Universidad de Cantabria
1.
Getino Redondo, María.
A search for new mechanisms to inhibit plasmid conjugation.
Degree: Máster U. en Biología Molecular y Biomedicina, 2011, Universidad de Cantabria
URL: http://hdl.handle.net/10902/553 
► Infections due to antibiotic-resistant (AbR) bacteria are a major cause of morbidity and mortality throughout the world. In addition, the number of new antibiotics being…
(more)
▼ Infections due to antibiotic-resistant (AbR) bacteria are a major cause of morbidity and mortality throughout the world. In addition, the number of new antibiotics being developed has plummeted. Although resistance genes can disseminate by any horizontal gene transfer mechanism, the vast majority of reports of bacterial gene transfer in the environment involve conjugation. Our group developed a method for high-throughput analysis of conjugation. This method was used to check for host genes in the recipient cell involved in conjugation, concluding that lab strains of Escherichia coli like DH5[alfa] have no non-essential genes that play an essential role in conjugation. In recipient cells, conjugation can be inhibited by different mechanisms: restriction systems, CRISPRs, entry exclusion systems or incompatibility, among others. Our aim is to screen a set of natural
plasmids to look for conjugation broad-range inhibitor genes in enterobacterial
plasmids, in order to hopefully identify new mechanisms of inhibition that could be used to control AbR propagation.
Advisors/Committee Members: Cruz Calahorra, Fernando de la (advisor), Universidad de Cantabria (other).
Subjects/Keywords: Plasmids
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APA (6th Edition):
Getino Redondo, M. (2011). A search for new mechanisms to inhibit plasmid conjugation. (Masters Thesis). Universidad de Cantabria. Retrieved from http://hdl.handle.net/10902/553
Chicago Manual of Style (16th Edition):
Getino Redondo, María. “A search for new mechanisms to inhibit plasmid conjugation.” 2011. Masters Thesis, Universidad de Cantabria. Accessed February 27, 2021.
http://hdl.handle.net/10902/553.
MLA Handbook (7th Edition):
Getino Redondo, María. “A search for new mechanisms to inhibit plasmid conjugation.” 2011. Web. 27 Feb 2021.
Vancouver:
Getino Redondo M. A search for new mechanisms to inhibit plasmid conjugation. [Internet] [Masters thesis]. Universidad de Cantabria; 2011. [cited 2021 Feb 27].
Available from: http://hdl.handle.net/10902/553.
Council of Science Editors:
Getino Redondo M. A search for new mechanisms to inhibit plasmid conjugation. [Masters Thesis]. Universidad de Cantabria; 2011. Available from: http://hdl.handle.net/10902/553
Oregon State University
2.
Quant, Rebecca L.
Molecular relationships among multiple-sized plasmids of Pseudomonas syringae pv. phaseolicola.
Degree: MS, Genetics, 1983, Oregon State University
URL: http://hdl.handle.net/1957/41745
► Five strains of the bean pathogen Pseudomonas syringae pv. phaseolicola were analyzed for plasmid number, size, and relatedness. Each strain contained one or two indigenous…
(more)
▼ Five strains of the bean pathogen Pseudomonas syringae pv.
phaseolicola were analyzed for plasmid number, size, and relatedness.
Each strain contained one or two indigenous
plasmids which ranged in
size from 6.8 to 151 kbp. BamHI and EcoRI restriction patterns revealed that total plasmid DNA from each strain had a high level of
sequence homology with pMC7105, a 148 kbp integrative plasmid found in
a sixth strain. Solution hybridization confirmed these results;
total plasmid DNA from each strain had 76 to 100 percent sequence
homology with pPP6220, a plasmid essentially identical to pMC7105.
The distribution of homology between pMC7105 and each plasmid was
determined from filter hybridizations. Only six BamHI fragments from
the
plasmids in these strains failed to hybridize with pMC7105 probe.
Four of these fragments, three from pPP6520 and one from pPP6525 of
strain PP652, hybridized strongly to plasmid DNA from a closely-related
pathovar, P. syringae pv. glycinea. BamHI fragment 8, which
is involved in the integration of pMC7105 into the host chromosome,
contains a repeat sequence that was present on all the
plasmids except pPP6120 (6.8 kbp), pPP6310 (40 kbp), and pPP6520 (45 kbp).
Every plasmid but pPP6520 had fragments that showed weak hybridization
to the small plasmid, pPP6120. This homology suggests that a second
repetitive sequence is common to these
plasmids. The large
plasmids
(148 to 151 kbp) were essentially identical to pMC7105. The intermediate
plasmids (122 to 128 kbp) appeared to be derived mainly from
pMC7105 or a related plasmid, whereas the smaller
plasmids (6.8 to
45 kbp) appear to have been derived in part from sequences not present
in pMC7105.
Advisors/Committee Members: Mills, Dallice I. (advisor), Becker, Robert (committee member).
Subjects/Keywords: Plasmids
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APA (6th Edition):
Quant, R. L. (1983). Molecular relationships among multiple-sized plasmids of Pseudomonas syringae pv. phaseolicola. (Masters Thesis). Oregon State University. Retrieved from http://hdl.handle.net/1957/41745
Chicago Manual of Style (16th Edition):
Quant, Rebecca L. “Molecular relationships among multiple-sized plasmids of Pseudomonas syringae pv. phaseolicola.” 1983. Masters Thesis, Oregon State University. Accessed February 27, 2021.
http://hdl.handle.net/1957/41745.
MLA Handbook (7th Edition):
Quant, Rebecca L. “Molecular relationships among multiple-sized plasmids of Pseudomonas syringae pv. phaseolicola.” 1983. Web. 27 Feb 2021.
Vancouver:
Quant RL. Molecular relationships among multiple-sized plasmids of Pseudomonas syringae pv. phaseolicola. [Internet] [Masters thesis]. Oregon State University; 1983. [cited 2021 Feb 27].
Available from: http://hdl.handle.net/1957/41745.
Council of Science Editors:
Quant RL. Molecular relationships among multiple-sized plasmids of Pseudomonas syringae pv. phaseolicola. [Masters Thesis]. Oregon State University; 1983. Available from: http://hdl.handle.net/1957/41745
Oregon State University
3.
Curiale, Michael Steven.
Physical properties and genetic studies of cryptic plasmids discovered in plant pathogenic pseudomonads.
Degree: PhD, Genetics, 1980, Oregon State University
URL: http://hdl.handle.net/1957/42138
► Six plant pathogenic isolates of Pseudomonas syringae pv. glycinea, the causal agent of bacterial blight of Glycine max (L.) Merrill, and one isolate of P.…
(more)
▼ Six plant pathogenic isolates of Pseudomonas
syringae pv. glycinea, the causal agent of bacterial
blight of Glycine max (L.) Merrill, and one isolate of
P. syringae pv. phaseolicola, the causal agent of halo
blight of Phaseolus vulgaris L., have been examined for
the presence of circular duplex deoxyribonucleic acid
(DNA). Extrachromosomal DNA from each strain was
first observed by cesium chloride-ethidium bromide dye-buoyant
density gradient centrifugation. The size and
number of
plasmids of four different isolates were
initially determined by neutral sucrose gradient
centrifugation. Subsequently, plasmid DNA from each
of the seven isolates was obtained by a cleared lysate
technique and analyzed by agarose gel electrophoresis.
Each of the isolates of P. syringae pv. glycinea harbor
a different complex array of
plasmids. The
plasmids
ranged from 3 to 109 megadaltons (Mdal) in size
and there were from two to seven discrete
plasmids per isolate. The isolate of P. syringae pv. phaseolicola
contained a single plasmid which had a molecular weight
of 98 Mdal.
Endonuclease-derived fingerprints of purified total
plasmid DNA from each isolate of P. syringae pv.
glycinea served as one criterion for measuring plasmid
relatedness among these pathovars of varying geographical
origin. Similar-sized fragments were present in
the digests of some of the isolates, but no single
fragment was observed to be present in the digests of
all isolates. Evidence for relatedness among the
plasmids
of these strains was obtained by hybridization of
nick-translated total plasmid DNA from one strain to
membrane blots of fingerprints of plasmid DNA from other
strains. Fifty-five to seventy-five percent of the
plasmid fragments from five strains hybridized with
radio-labeled plasmid probe prepared from a sixth
strain. In reciprocal hybridizations, radio-labeled
probe prepared from each of four strains hybridized to
a specific fragment of pMC10 plasmid and to three fragments
of pMC11 plasmid, which were present in the digests
of the sixth strain. Other fragments of pMC10
and pMC11 showed hybridization to labeled probe from
some of the strains, but not with others.
The plasmid, pMC7105, of a mutant of P. syringae
pv. phaseolicola integrated into the bacterial chromosome.
Biochemical evidence for integration was obtained by demonstrating that all of the plasmid sequence was
present in a plasmidless isolate when membrane blots of
total restriction endonuclease-cleaved DNA was probed
with radio-labeled pMC7105 DNA. Furthermore, new
autonomous
plasmids have been detected in three strains
which have resulted from partial excision of pMC7105.
The probable insertion locus of p1/iC7105 and regions of
excision for one of the derived
plasmids were surmised
from fingerprint analysis of plasmid DNA.
The wide host-range antibiotic- resistance - plasmid
RP4 was transferred by conjugation from Escherichia coli
to strains of both pathovars. The RP4-containing
strains of P. syringae pv. glycinea produced RP4-less
…
Advisors/Committee Members: Mills, Dallice (advisor), Brown, Lyle (committee member).
Subjects/Keywords: Plasmids
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APA (6th Edition):
Curiale, M. S. (1980). Physical properties and genetic studies of cryptic plasmids discovered in plant pathogenic pseudomonads. (Doctoral Dissertation). Oregon State University. Retrieved from http://hdl.handle.net/1957/42138
Chicago Manual of Style (16th Edition):
Curiale, Michael Steven. “Physical properties and genetic studies of cryptic plasmids discovered in plant pathogenic pseudomonads.” 1980. Doctoral Dissertation, Oregon State University. Accessed February 27, 2021.
http://hdl.handle.net/1957/42138.
MLA Handbook (7th Edition):
Curiale, Michael Steven. “Physical properties and genetic studies of cryptic plasmids discovered in plant pathogenic pseudomonads.” 1980. Web. 27 Feb 2021.
Vancouver:
Curiale MS. Physical properties and genetic studies of cryptic plasmids discovered in plant pathogenic pseudomonads. [Internet] [Doctoral dissertation]. Oregon State University; 1980. [cited 2021 Feb 27].
Available from: http://hdl.handle.net/1957/42138.
Council of Science Editors:
Curiale MS. Physical properties and genetic studies of cryptic plasmids discovered in plant pathogenic pseudomonads. [Doctoral Dissertation]. Oregon State University; 1980. Available from: http://hdl.handle.net/1957/42138
Oregon State University
4.
Tsai, Huai-jen.
Conjugal transfer of plasmid genes between lactic Streptococcus and Leuconostoc starter bacteria.
Degree: PhD, Microbiology, 1986, Oregon State University
URL: http://hdl.handle.net/1957/40592
► Mutants of Streptococcus lactis 7962 with various combinations of plasmid molecular masses were screened for nisin production. Nisin was produced by both wild type and…
(more)
▼ Mutants of Streptococcus lactis 7962 with various combinations of plasmid molecular masses were screened for nisin production. Nisin was produced by both wild type and mutants that contained a 17.5 Md plasmid, which was usually obscured by the chromosomal fragments. No nisin was produced by plasmid-free mutants. Sucrose fermentation and nisin production were related. A transconjugant obtained from nisin-producing donor S. lactis 7962 and recipient Leuconostoc dextranicum 181 was a "super-nisin" producer. Like the recipient, the transconjugant was resistant to both 7962 phage and to vancomycin (>1,000 ug/ml); but the plasmid profile showed an extra 17.5 Md plasmid. Conjugation between Lac⁺ S. lactis C2 and Lac⁻ L. cremoris CAF7 was also performed. It was a high-frequency conjugation type. Transconjugants fermented lactose significantly faster than did the wild type. When grown in litmus milk fortified with 0.2% yeast extract Lac⁺ transconjugants reached pH 4.68 within 24 h at 30 C: they were also positive for diacetyl production. The Lac⁺ Leuconostoc were resistant to c2 phage and also to >500 ug/ml vancomycin. Plasmid profiles of 10 Lac⁺ transconjugants showed two unique patterns. A novel enlarged plasmid was found. Application of Southern blot hybridization technique revealed some homology with the 30 Md plasmid of donor, recipient and the transconjugants as well as with some of the remaining
plasmids of the donor.
Advisors/Committee Members: Sandine, William E. (advisor).
Subjects/Keywords: Plasmids
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APA (6th Edition):
Tsai, H. (1986). Conjugal transfer of plasmid genes between lactic Streptococcus and Leuconostoc starter bacteria. (Doctoral Dissertation). Oregon State University. Retrieved from http://hdl.handle.net/1957/40592
Chicago Manual of Style (16th Edition):
Tsai, Huai-jen. “Conjugal transfer of plasmid genes between lactic Streptococcus and Leuconostoc starter bacteria.” 1986. Doctoral Dissertation, Oregon State University. Accessed February 27, 2021.
http://hdl.handle.net/1957/40592.
MLA Handbook (7th Edition):
Tsai, Huai-jen. “Conjugal transfer of plasmid genes between lactic Streptococcus and Leuconostoc starter bacteria.” 1986. Web. 27 Feb 2021.
Vancouver:
Tsai H. Conjugal transfer of plasmid genes between lactic Streptococcus and Leuconostoc starter bacteria. [Internet] [Doctoral dissertation]. Oregon State University; 1986. [cited 2021 Feb 27].
Available from: http://hdl.handle.net/1957/40592.
Council of Science Editors:
Tsai H. Conjugal transfer of plasmid genes between lactic Streptococcus and Leuconostoc starter bacteria. [Doctoral Dissertation]. Oregon State University; 1986. Available from: http://hdl.handle.net/1957/40592
McMaster University
5.
Oloni, Martins.
New Delhi Metallo-beta-lactamase (NDM) plasmid predictions.
Degree: MSc, 2020, McMaster University
URL: http://hdl.handle.net/11375/25906
► The complete detection and genome sequencing of resistance plasmids is required to understand the co-occurrence of resistance genes on plasmids and their transmission, which is…
(more)
▼ The complete detection and genome sequencing of resistance plasmids is required to understand the co-occurrence of resistance genes on plasmids and their transmission, which is necessary to mitigate the spread of drug resistant bacterial infections. One group of multi-drug resistance plasmids of interest are the New Delhi Metallo--lactamase (NDM) associated plasmids. Bacteria pathogens with NDM genes are resistant to a broad range of beta-lactam antibiotics, including carbapenems, a mainstay for treating bacterial infections. We sequenced clinical isolates collected from patients that failed to respond to antibiotic treatments in the Hamilton-Niagara community using Next Generation Sequencing (NGS) technology, which is the standard technique for sequencing bacterial genomes. I analyzed their assembled genomes via the development of a new resistance plasmid prediction bioinformatics pipeline: RGI:Mobilome. RGI:Mobilome predicted NDM bearing plasmids in 15 isolates, along with other resistance genes on plasmids. However, the NDM plasmid predictions of all 15 isolates were fragmented due to incomplete genome assemblies, caused by repetitive sequences within bacterial genomes and use of Illumina sequencing technology. However, plasmid predictions greatly improved when we leveraged the high nucleotide accuracy of Illumina reads and the structural resolving power of long reads generated with the Oxford Nanopore Technology (ONT) to produce ‘hybrid’ genome assemblies. From the 15 putative hybrid-assembly NDM plasmids, one plasmid was a complete match to a known pKP-NDM1 plasmid, seven plasmids were “variants” of known and well-characterized plasmids, and the remaining seven plasmids were “distant homologs” of known and well-characterized plasmids, suggesting previously undescribed NDM-associated plasmids in our community. This thesis project reveals the diversity of NDM plasmid types within the Hamilton-Niagara community, which is valuable to epidemiologists and public health practitioners to devise actionable plans required to mitigate the spread of multi-drug resistance NDM plasmids and for clinicians to treat infections caused by NDM positive strains.
Thesis
Master of Science (MSc)
The spread of antimicrobial resistance often arises from the exchange of resistance plasmids between bacterial species in their immediate environment. One resistance gene carried on plasmids is the New Delhi Metallo-beta-lactamase (NDM) gene, which confers resistance to almost all beta-lactam antibiotics. To control the spread of NDM plasmids, we need to detect them completely. Using genome sequencing, we analyzed clinical isolates from patients in hospitals within the Hamilton-Niagara community for NDM plasmids. From our results, we detected one complete match to a known NDM plasmid. We also detected seven NDM-associated plasmids that are close variants of known NDM plasmids, plus another seven NDM-associated plasmid that we classified as ‘novel’ since they are widely different from known NDM plasmids. This thesis reveals the diverse…
Advisors/Committee Members: McArthur, Andrew, Biochemistry and Biomedical Sciences.
Subjects/Keywords: NDM plasmids
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APA (6th Edition):
Oloni, M. (2020). New Delhi Metallo-beta-lactamase (NDM) plasmid predictions. (Masters Thesis). McMaster University. Retrieved from http://hdl.handle.net/11375/25906
Chicago Manual of Style (16th Edition):
Oloni, Martins. “New Delhi Metallo-beta-lactamase (NDM) plasmid predictions.” 2020. Masters Thesis, McMaster University. Accessed February 27, 2021.
http://hdl.handle.net/11375/25906.
MLA Handbook (7th Edition):
Oloni, Martins. “New Delhi Metallo-beta-lactamase (NDM) plasmid predictions.” 2020. Web. 27 Feb 2021.
Vancouver:
Oloni M. New Delhi Metallo-beta-lactamase (NDM) plasmid predictions. [Internet] [Masters thesis]. McMaster University; 2020. [cited 2021 Feb 27].
Available from: http://hdl.handle.net/11375/25906.
Council of Science Editors:
Oloni M. New Delhi Metallo-beta-lactamase (NDM) plasmid predictions. [Masters Thesis]. McMaster University; 2020. Available from: http://hdl.handle.net/11375/25906
University of Manitoba
6.
Sagert, Jason.
Characterization of Listeria monocytogenes plasmids that were newly identified in whole-genome sequences of listeriosis outbreak isolates.
Degree: Medical Microbiology, 2014, University of Manitoba
URL: http://hdl.handle.net/1993/23160
► Listeria monocytogenes is a Gram-positive bacterium that is found ubiquitously throughout nature and is the etiologic agent of listeriosis. The majority of human listeriosis is…
(more)
▼ Listeria monocytogenes is a Gram-positive bacterium that is found ubiquitously throughout nature and is the etiologic agent of listeriosis. The majority of human listeriosis is foodborne, resulting from the consumption of unpasteurized and ready-to-eat foods that are contaminated during food processing. During the 2008 nationwide outbreak, the Gilmour laboratory performed the first real-time application of high-throughput whole-genome sequencing (WGS) of outbreak strains. Within this genomic data, the 77 kb plasmid, pLM5578, was newly identified in a clinical isolate, and additional Listeria
plasmids (the 80 kb pLM5026 and the 60 kb pLM0813) were subsequently identified after WGS was completed on an expanded panel of outbreak isolates. Little was known regarding how
plasmids contribute to persistence and virulence of L. monocytogenes, and to investigate these potential relationships, a panel of 147 L. monocytogenes food, environmental, and clinical isolates from Canadian public health events from the last three decades was selected for further study of the
plasmids they might contain. Strain carriage of
plasmids was determined using conventional PCR targeting known plasmid gene targets. Bioinformatic analyses were then used to predict the functions of individual genes encoded by each sequenced plasmid. These analyses were then used to direct experiments investigating the functions and associated phenotypes conferred by plasmid carriage. Phenotypic analyses included antimicrobial susceptibility testing, heavy metal resistance, and biofilm formation assays. Finally, WGS analyses was performed on isolates with plasmid screening patterns that indicated carriage of potential novel
plasmids. Screening revealed that 75 of 147 isolates were positive for the presence of a plasmid, for which WGS analysis identified 24 unique newly identified L. monocytogenes
plasmids. Phenotypically, 15 of these
plasmids were found to contribute to a decreased susceptibility to the heavy metal cadmium, whereas 4 conferred resistance to the sanitizer benzalkonium chloride. Plasmid carriage was also found to affect biofilm formation. Nine
plasmids correlated with stronger biofilm formation phenotypes; whereas 5
plasmids were correlated with weaker biofilm formation phenotypes. No known virulence factors or antibiotic resistance determinants were present in the DNA sequences of these 24 newly identified
plasmids. Numerous coding sequences predicted to assist with survival under environmental stress were identified, and it is hypothesized that these
plasmids likely contributed to persistence of L. monocytogenes within food processing environments.
Advisors/Committee Members: Gilmour, Matthew (Medical Microbiology) (supervisor), Wylie, John (Medical Microbiology) Graham, Morag (Medical Microbiology) Van Domselaar, Gary (Medical Microbiology) Holley, Rick (Food Science) (examiningcommittee).
Subjects/Keywords: Listeria monocytogenes; plasmids
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APA (6th Edition):
Sagert, J. (2014). Characterization of Listeria monocytogenes plasmids that were newly identified in whole-genome sequences of listeriosis outbreak isolates. (Masters Thesis). University of Manitoba. Retrieved from http://hdl.handle.net/1993/23160
Chicago Manual of Style (16th Edition):
Sagert, Jason. “Characterization of Listeria monocytogenes plasmids that were newly identified in whole-genome sequences of listeriosis outbreak isolates.” 2014. Masters Thesis, University of Manitoba. Accessed February 27, 2021.
http://hdl.handle.net/1993/23160.
MLA Handbook (7th Edition):
Sagert, Jason. “Characterization of Listeria monocytogenes plasmids that were newly identified in whole-genome sequences of listeriosis outbreak isolates.” 2014. Web. 27 Feb 2021.
Vancouver:
Sagert J. Characterization of Listeria monocytogenes plasmids that were newly identified in whole-genome sequences of listeriosis outbreak isolates. [Internet] [Masters thesis]. University of Manitoba; 2014. [cited 2021 Feb 27].
Available from: http://hdl.handle.net/1993/23160.
Council of Science Editors:
Sagert J. Characterization of Listeria monocytogenes plasmids that were newly identified in whole-genome sequences of listeriosis outbreak isolates. [Masters Thesis]. University of Manitoba; 2014. Available from: http://hdl.handle.net/1993/23160
Ryerson University
7.
Shamsad, Farhana.
Transfer of the Catabolic Plasmid Pjp4 in Bacterial Biofilms in Lab-Scale Continuous Flow-Through Cells.
Degree: 2012, Ryerson University
URL: https://digital.library.ryerson.ca/islandora/object/RULA%3A2119
► Limited information is available on how external environmental factors (e.g., the type of carbon source) affect biofilm architecture, conjugative transfer of the plasmid pJP4 and…
(more)
▼ Limited information is available on how external environmental factors (e.g., the type of carbon source) affect biofilm architecture, conjugative transfer of the plasmid pJP4 and xenobiotic degradation in biofilms. The aim of this project was to investigate the influence of glucose and 2,4-dichlorophenoxyacetic acid, two different carbon sources which represent the absence and presence of selective pressure, respectively, on the combined effect of biofilm architecture, transfer of the plasmid pJP4 in soil derived mixed culture biofilms and consequent biodegradation of 2,4-D. The pJP4 plasmid was transferred to soil-derived mixed culture recipients in plate mating experiments and isolated transconjugant colonies were characterized as Comamonas testosteroni. Donor and transconjugant cells were not detected microscopically in biofilms and no transconjugant colonies were isolated; however, gfp, dsRed, and tfdB genes were detected in biofilm effluents with and without selective pressure. Heterogeneous biofilm architecture was observed for both with and without selective pressure.
Advisors/Committee Members: Ryerson University (Degree grantor).
Subjects/Keywords: Biofilms; Biofilms – Analysis; Plasmids; Microbiology
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APA (6th Edition):
Shamsad, F. (2012). Transfer of the Catabolic Plasmid Pjp4 in Bacterial Biofilms in Lab-Scale Continuous Flow-Through Cells. (Thesis). Ryerson University. Retrieved from https://digital.library.ryerson.ca/islandora/object/RULA%3A2119
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Shamsad, Farhana. “Transfer of the Catabolic Plasmid Pjp4 in Bacterial Biofilms in Lab-Scale Continuous Flow-Through Cells.” 2012. Thesis, Ryerson University. Accessed February 27, 2021.
https://digital.library.ryerson.ca/islandora/object/RULA%3A2119.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Shamsad, Farhana. “Transfer of the Catabolic Plasmid Pjp4 in Bacterial Biofilms in Lab-Scale Continuous Flow-Through Cells.” 2012. Web. 27 Feb 2021.
Vancouver:
Shamsad F. Transfer of the Catabolic Plasmid Pjp4 in Bacterial Biofilms in Lab-Scale Continuous Flow-Through Cells. [Internet] [Thesis]. Ryerson University; 2012. [cited 2021 Feb 27].
Available from: https://digital.library.ryerson.ca/islandora/object/RULA%3A2119.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Shamsad F. Transfer of the Catabolic Plasmid Pjp4 in Bacterial Biofilms in Lab-Scale Continuous Flow-Through Cells. [Thesis]. Ryerson University; 2012. Available from: https://digital.library.ryerson.ca/islandora/object/RULA%3A2119
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Rhodes University
8.
Mendes, Adriano.
A baculovirus-mediated expression system for the analysis of HaSV RNA packaging.
Degree: Faculty of Science, Biochemistry, Microbiology and Biotechnology, 2012, Rhodes University
URL: http://hdl.handle.net/10962/d1004085
► The Helicoverpa armigera stunt virus (HaSV) is a member of a family of small nonenveloped (+) ssRNA insect viruses currently known as the Tetraviridae. This…
(more)
▼ The Helicoverpa armigera stunt virus (HaSV) is a member of a family of small nonenveloped (+) ssRNA insect viruses currently known as the Tetraviridae. This family is unique in terms of the T=4 quasi-symmetry of its capsid particles and the unusually narrow host range and tissue tropism. Assembly of tetraviral particles has been well characterised and involves the combination of 240 copies of a single capsid precursor protein (VCap) into a procapsid followed by autoproteolytic cleavage to yield the major (β) and minor (γ) capsid subunits within the mature particle. HaSV has two genomic RNAs, RNA 1 encoding the replicase and RNA 2 encoding VCap and p17, the ORF of which lies upstream of and overlaping with the 5’ end of the VCap ORF. Prior to this study, Vlok (2009) used a plasmid expression system to study RNA packaging in HaSV VLPs assembled in Spodoptera frugiperda 9 (Sf9) cells co-expressing p17 and VCap. The study showed that the p17 ORF was required for the packaging of RNA 2 during capsid assembly but it was unclear whether p17 expression was required for packaging. In addition, expression from the transfected plasmids was sub-optimal affecting both the yield of VLPs and the detection of p17. The aim of this study was to use the plasmid system to test whether p17 expression was required for plasmid-derived VLP RNA packaging and then develop a baculovirus-mediated system to test this hypothesis. By using a plasmid in which the start codon of p17 was mutated, it was shown that p17 expression was required for RNA 2 packaging into plasmid-VLPs. For the baculovirus system, four recombinant baculoviruses based upon the pFastBac Dual expression system, were constructed. These included Bac20, expressing wild type RNA 2, Bac21, RNA 2 with p17 silenced, Bac23, RNA 2 and p17 expressed on a separate transcript and Bac24, RNA 2 with p17 silenced plus p17 expressed on a separate transcript. Assembly of VLPs was more efficient using the baculovirus expression system and p17 expression was observed in cells infected with Bac20, Bac23 and Bac24, but not Bac21. In contrast to the plasmid-VLPs, bac-VLPs did not require p17 for the encapsidation of RNA 2. In addition to RNA 2, Bac23 and Bac24 packaged the p17 mRNA transcribed separately from RNA 2. This insinuated that bac-VLPs may be packaging RNA non-selectively. It was proposed that p17 may play a role in packaging in an RNA-limiting environment (plasmid system) but functioned differently when viral RNA was in excess (baculovirus system). This data points to the importance of developing a replication system for the analysis of the packaging pathways of these viruses and this study has laid down the foundations for such a system in which RNA 1 and RNA 2 can be introduced into a single cell by means of a single recombinant virus.
Subjects/Keywords: RNA; Baculoviruses; Helicoverpa armigera; Plasmids
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APA (6th Edition):
Mendes, A. (2012). A baculovirus-mediated expression system for the analysis of HaSV RNA packaging. (Thesis). Rhodes University. Retrieved from http://hdl.handle.net/10962/d1004085
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Mendes, Adriano. “A baculovirus-mediated expression system for the analysis of HaSV RNA packaging.” 2012. Thesis, Rhodes University. Accessed February 27, 2021.
http://hdl.handle.net/10962/d1004085.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Mendes, Adriano. “A baculovirus-mediated expression system for the analysis of HaSV RNA packaging.” 2012. Web. 27 Feb 2021.
Vancouver:
Mendes A. A baculovirus-mediated expression system for the analysis of HaSV RNA packaging. [Internet] [Thesis]. Rhodes University; 2012. [cited 2021 Feb 27].
Available from: http://hdl.handle.net/10962/d1004085.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Mendes A. A baculovirus-mediated expression system for the analysis of HaSV RNA packaging. [Thesis]. Rhodes University; 2012. Available from: http://hdl.handle.net/10962/d1004085
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Stellenbosch University
9.
Van Zyl, Leonardo Joaquim.
Analysis of the mobilization region of the broad host-range IncQ-like plasmid, pTC-F14, and its ability to interact with a related plasmid, pTF-FC2.
Degree: MSc, Microbiology, 2003, Stellenbosch University
URL: http://hdl.handle.net/10019.1/70109
► ENGLISH ABSTRACT: The 14.2 kb plasmid pTC-FI4 was isolated from the moderately thermophilic (45°- 50°C), highly acidophilic (pH 1.5 to 2.5), chemolithotrophic bacterium Acidithiobacil/us caldus…
(more)
▼ ENGLISH ABSTRACT: The 14.2 kb plasmid pTC-FI4 was isolated from the moderately thermophilic (45°-
50°C), highly acidophilic (pH 1.5 to 2.5), chemolithotrophic bacterium
Acidithiobacil/us caldus and has a replicon that is closely related to the promiscuous,
broad host-range, IncQ-family of plasmids. The region containing the mobilization
genes was sequenced and encoded five Mob proteins and an origin of transfer, which
are related to the DNA processing (Tral) region of IncPI plasmids, rather than to the
three Mob protein systems of the IncQ-l-group plasmids (e.g. plasmids RSFIOIO or
R1162). Plasmid pTC-F14 is the third example of an IncQ family plasmid that has
five mob genes, with the others being pTF-FC2 and pRAS3.1. The minimal region
that was essential for mobilization included the mobA, mobB and the mobC genes as
well as the oriT. The mobD and mobE genes were non-essential, but together
enhanced the mobilization frequency by approximately 300-fold. The repB gene
increased the mobilization frequency but was not essential for mobilization.
Mobilization of pTC-F14 between Escherichia coli strains by a chromosomally
integrated RP4 plasmid was more than 3500-fold less efficient than the mobilization
ofpTF-FC2. When both plasmids were co-resident in the same E. coli host, pTC-FI4
was mobilized at almost the same frequency as pTF-FC2. This enhanced pTC-FI4
mobilization frequency was due to the presence of a combination of the pTF-FC2
mobD and mobE gene products, the functions of which are still unknown. pTF-FC2
could mobilize the oriT of pTC-FI4 whereas pTC-F14 could only mobilize the pTFFC2
oriT if provided with some of the mobilization genes from the pTC-FC2
mobilization region. Unexpectedly either the mobEDC genes or the mobAB genes
would allow the mobilization of the pTF-FC2 oriT by pTC-F14 even though there
was no common gene between the two subsets. No evidence for any negative effect
on the transfer of one plasmid by the related, potentially competitive plasmid was
obtained.
AFRIKAANSE OPSOMMING: Die 14.2 kb plasmied, pTC-F14, is uit die matig termofiliese (45°C tot 50°C), hoogs
asidofiliese (pH 1.5 tot 2.5), chemolitooutotrofiese bakterium Acidithiobaci/lus caldus
geisoleer en beskik oor 'n replikon wat verwant is aan die vanaf die IncQ-familie van
plasmiede. Hierdie plasmiede is alom bekend vir hulle promiskuïteit tydens
konjugasie asook hul vermoë om in 'n groot aantal verskillende gasheer organismes te
kan repliseer. DNA volgorde analise van die mobiliserings area het 'n oordrags
oorsprong asook vyf oop leesrame onthul wat nader verwant is aan die DNA
prosseserings gene van die Tral area op die IncP 1 plasmiede, as die van die
mobiliserings stelsel van die IncQ-l-groep plasmiede. Plasmied pTC-Fl4 is die derde
voorbeeld, saam met pTF-FC2 en pRAS3.1, van 'n IncQ-tipe plasmied met 'n vyfgeen
mobiliserings sisteem. Die kleinste area op die plasmied nodig vir mobilisering
van pTC-Fl4 is bepaal, en het die mobA, mobB en mobC gene sowel as die oordrags
oorsprong ingesluit.…
Advisors/Committee Members: Rawlings, D. E., Stellenbosch University. Faculty of Science. Dept. of Microbiology..
Subjects/Keywords: Plasmids – Genetics
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APA (6th Edition):
Van Zyl, L. J. (2003). Analysis of the mobilization region of the broad host-range IncQ-like plasmid, pTC-F14, and its ability to interact with a related plasmid, pTF-FC2. (Masters Thesis). Stellenbosch University. Retrieved from http://hdl.handle.net/10019.1/70109
Chicago Manual of Style (16th Edition):
Van Zyl, Leonardo Joaquim. “Analysis of the mobilization region of the broad host-range IncQ-like plasmid, pTC-F14, and its ability to interact with a related plasmid, pTF-FC2.” 2003. Masters Thesis, Stellenbosch University. Accessed February 27, 2021.
http://hdl.handle.net/10019.1/70109.
MLA Handbook (7th Edition):
Van Zyl, Leonardo Joaquim. “Analysis of the mobilization region of the broad host-range IncQ-like plasmid, pTC-F14, and its ability to interact with a related plasmid, pTF-FC2.” 2003. Web. 27 Feb 2021.
Vancouver:
Van Zyl LJ. Analysis of the mobilization region of the broad host-range IncQ-like plasmid, pTC-F14, and its ability to interact with a related plasmid, pTF-FC2. [Internet] [Masters thesis]. Stellenbosch University; 2003. [cited 2021 Feb 27].
Available from: http://hdl.handle.net/10019.1/70109.
Council of Science Editors:
Van Zyl LJ. Analysis of the mobilization region of the broad host-range IncQ-like plasmid, pTC-F14, and its ability to interact with a related plasmid, pTF-FC2. [Masters Thesis]. Stellenbosch University; 2003. Available from: http://hdl.handle.net/10019.1/70109
University of Minnesota
10.
Lang, Kevin.
Transcriptional regulation of incompatibility type A/C plasmids.
Degree: PhD, Comparative and Molecular Biosciences, 2015, University of Minnesota
URL: http://hdl.handle.net/11299/174866
► Plasmids are extrachromosomal DNA elements that often carry beneficial phenotypes for the bacterial host. Incompatibility type A/C (IncA/C) plasmids are large (~100 ~ 200 kilobases…
(more)
▼ Plasmids are extrachromosomal DNA elements that often carry beneficial phenotypes for the bacterial host. Incompatibility type A/C (IncA/C) plasmids are large (~100 ~ 200 kilobases (kb)), conjugative plasmids that are carried by Gram-negative bacteria. IncA/C plasmids often carry numerous genes that confer resistance to antimicrobials and have been isolated from many types of bacteria that pose significant risks in both human and animal medicine. Although IncA/C plasmids have been described in the literature for many years, little is known about their basic biology. For the past decade, many fully sequenced IncA/C variants have been described. There has been a lack of work concerning core functions of these plasmids, such as, replication, conjugative transfer and maintenance. This dissertation focuses on how these plasmids are regulated on a transcriptional level. We used pAR060302, a prototypical IncA/C plasmid, to conduct several experiments investigating exactly what genes are transcribed and how different conditions affect their transcriptional landscape. RNA-Seq was used to understand how antimicrobial exposure can affect the way genes are expressed on IncA/C plasmids. We found that, under the conditions we tested, antimicrobials have little effect on the transcription of genes on pAR060302. However, this initial study was the first to explore genes carried IncA/C plasmids in terms of their expression. Further RNA-Seq experiments were carried out in several different bacterial genera all carrying the same IncA/C plasmid, pAR060302. These experiments attempted to characterize how a broad host-range plasmid, such as IncA/C, might be differentially regulated in different hosts. We found that only subtle changes occur in the expression of plasmid genes. Carriage of IncA/C plasmids was found to have diverse effects on chromosomal gene expression. Genes involved in 2-carbon metabolism in Escherichia coli are up-regulated due to plasmid carriage. Our results suggest that plasmid encoded factors might serve varying levels of importance depending on the host chromosomal background. Experiments were carried out on E. coli carrying plasmids with mutations in a group of predicted transcriptional regulators to determine their function. We identified the positive regulators of conjugative transfer in IncA/C plasmids, acaD and acaC. We also found a repressor of transfer, acr2, which encodes an H-NS-like protein. We further show that acr2 might regulate genes beyond those that are involved in conjugative transfer. This dissertation builds on our understanding on what mechanisms are important for the maintenance of large plasmids in Gram-negative bacteria. Understanding how plasmids might specifically tune host metabolism to improve competitive fitness would impact what evolutionary processes were involved in their emergence. Characterization of regulatory networks that govern core plasmid processes, such as conjugation, might assist in the development of new models of how these plasmids disseminate throughout bacterial…
Subjects/Keywords: antibiotic resistance; gene regulation; Plasmids
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APA (6th Edition):
Lang, K. (2015). Transcriptional regulation of incompatibility type A/C plasmids. (Doctoral Dissertation). University of Minnesota. Retrieved from http://hdl.handle.net/11299/174866
Chicago Manual of Style (16th Edition):
Lang, Kevin. “Transcriptional regulation of incompatibility type A/C plasmids.” 2015. Doctoral Dissertation, University of Minnesota. Accessed February 27, 2021.
http://hdl.handle.net/11299/174866.
MLA Handbook (7th Edition):
Lang, Kevin. “Transcriptional regulation of incompatibility type A/C plasmids.” 2015. Web. 27 Feb 2021.
Vancouver:
Lang K. Transcriptional regulation of incompatibility type A/C plasmids. [Internet] [Doctoral dissertation]. University of Minnesota; 2015. [cited 2021 Feb 27].
Available from: http://hdl.handle.net/11299/174866.
Council of Science Editors:
Lang K. Transcriptional regulation of incompatibility type A/C plasmids. [Doctoral Dissertation]. University of Minnesota; 2015. Available from: http://hdl.handle.net/11299/174866
University of British Columbia
11.
Smith, Richard D.
Isolation and characterization of plasmids carrying genes of bacteriophage T7.
Degree: MS- MSc, Microbiology and Immunology, 1978, University of British Columbia
URL: http://hdl.handle.net/2429/21468
► The restriction endonuclease Hpa I cleaves wild type T7 DNA into nineteen fragments. These Hpa I fragments were inserted into the ampicillin resistance gene of…
(more)
▼ The restriction endonuclease Hpa I cleaves wild type T7 DNA into nineteen fragments. These Hpa I fragments were inserted into the ampicillin resistance gene of the plasmid pBR322. The poly A - poly T method of construction was chosen in order to insure single T7 DNA inserts.
Two hundred fifty clones were identified as carrying T7 DNA segments
by colony hybridization with ³²P T7⁺ DNA. From these, clones carrying specific areas of the T7 genome were identified by various methods described in this thesis. Clones carrying Hpa I fragments E and G were identified by hybridization with Southern filters of electrophoressed Hpa I digested T7 DNA. Clones carrying most of gene 5 and parts of gene 4 were identified by hybridization with a purified restriction fragment carrying those genes (Hpa I fragment D). Clones containing parts of genes 10 and 11 were identified by marker rescue tests. Marker rescue "spot tests" were developed as a fast screening procedure for T7 genes and rely on generalized recombination between the T7 segment carried by the plasmid and the infecting phage DNA.
Subjects/Keywords: Plasmids; Bacteriophages
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APA (6th Edition):
Smith, R. D. (1978). Isolation and characterization of plasmids carrying genes of bacteriophage T7. (Masters Thesis). University of British Columbia. Retrieved from http://hdl.handle.net/2429/21468
Chicago Manual of Style (16th Edition):
Smith, Richard D. “Isolation and characterization of plasmids carrying genes of bacteriophage T7.” 1978. Masters Thesis, University of British Columbia. Accessed February 27, 2021.
http://hdl.handle.net/2429/21468.
MLA Handbook (7th Edition):
Smith, Richard D. “Isolation and characterization of plasmids carrying genes of bacteriophage T7.” 1978. Web. 27 Feb 2021.
Vancouver:
Smith RD. Isolation and characterization of plasmids carrying genes of bacteriophage T7. [Internet] [Masters thesis]. University of British Columbia; 1978. [cited 2021 Feb 27].
Available from: http://hdl.handle.net/2429/21468.
Council of Science Editors:
Smith RD. Isolation and characterization of plasmids carrying genes of bacteriophage T7. [Masters Thesis]. University of British Columbia; 1978. Available from: http://hdl.handle.net/2429/21468
University of Alberta
12.
Ahn, Cheol.
Bacteriocin production in Carnobacterium piscicola LV17 and
location of its genetic determinant.
Degree: PhD, Department of Food Science, 1991, University of Alberta
URL: https://era.library.ualberta.ca/files/70795b32d
Subjects/Keywords: Plasmids – Genetics.; Plasmids.; Bacteriocins.
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APA (6th Edition):
Ahn, C. (1991). Bacteriocin production in Carnobacterium piscicola LV17 and
location of its genetic determinant. (Doctoral Dissertation). University of Alberta. Retrieved from https://era.library.ualberta.ca/files/70795b32d
Chicago Manual of Style (16th Edition):
Ahn, Cheol. “Bacteriocin production in Carnobacterium piscicola LV17 and
location of its genetic determinant.” 1991. Doctoral Dissertation, University of Alberta. Accessed February 27, 2021.
https://era.library.ualberta.ca/files/70795b32d.
MLA Handbook (7th Edition):
Ahn, Cheol. “Bacteriocin production in Carnobacterium piscicola LV17 and
location of its genetic determinant.” 1991. Web. 27 Feb 2021.
Vancouver:
Ahn C. Bacteriocin production in Carnobacterium piscicola LV17 and
location of its genetic determinant. [Internet] [Doctoral dissertation]. University of Alberta; 1991. [cited 2021 Feb 27].
Available from: https://era.library.ualberta.ca/files/70795b32d.
Council of Science Editors:
Ahn C. Bacteriocin production in Carnobacterium piscicola LV17 and
location of its genetic determinant. [Doctoral Dissertation]. University of Alberta; 1991. Available from: https://era.library.ualberta.ca/files/70795b32d
University of Utah
13.
Murphy, Maria A.
Genetic exchange among linear replicons of the lyme disease spirochete, Borrelia burgdorferi;.
Degree: MS;, Oncological Sciences;, 1995, University of Utah
URL: http://content.lib.utah.edu/cdm/singleitem/collection/etd1/id/543/rec/578
► Spirochetes of the genus Borrelia are unusual among members of the prokaryotic kingdom as they have a linear chromosome of 950±20 kb. Initial physical mapping…
(more)
▼ Spirochetes of the genus Borrelia are unusual among members of the prokaryotic kingdom as they have a linear chromosome of 950±20 kb. Initial physical mapping studies of Borrelia species indicate that this linear chromosome is stable with the exception of some observed variability at the right end of the linear chromosome. Among the original seven B. burgdorferi isolates analyzed the terminal SgrAI restriction fragment was found to have three possible sizes, thus implying that some isolates carry extra DNA at the right end of the chromosome. A panel of 30 B. burgdorferi isolates was obtained to allow further investigation of this phenomenon. Isolates were first cleaved with SgrAI to determine the length of the terminal restriction fragment. These experiments demonstrated that there are four possible lengths for the B. burgdorferi chromosome. Long length chromosome isolates carry 14 kb of extra DNA that is not present on short chromosome isolates; longer and intermediate length isolates carry 12 kb and 6 kb of DNA respectively. DNA found on intermediate, longer and long length chromosome isolates but not on short chromosome isolates was termed right-end extension sequence. Cloning and sequencing of a 1.6 kb DNA fragment (from isolate 1352), which begins in sequence conserved in all chromosome lengths and extends into right-end extension sequence, revealed that chromosomal right-end extension sequence is carried on plasmids in 13 out of 30 isolates. The 30 members of the panel can carry zero, one or two copies of this cloned right-end extension sequence. When only one copy is present, it may be either on the chromosome or a linear plasmid. Further analysis of the right end revealed that some isolates carry up to 6 kb of chromosomal DNA on linear plasmids. The prevalence and conservation of these 6-14 kb right-end extension sequences imply that they must be biologically significant. Isolates representative of the other eight species do not have right-end extension sequence. B. burgdorferi must have acquired this extra DNA since diverging from a common Borrelia ancestor. The presence of B. burgdorferi chromosomal DNA on linear plasmids suggest that chromosome telomeres may be exchanging with plasmid telomeres through a single recombination event. These dynamic events may contribute to the organism’s pathogenicity, and ability to avoid the host immune response or allow the organism to alternate between life in an aartropod vector and a mammalian host.
Subjects/Keywords: DNA; Linear Plasmids
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APA (6th Edition):
Murphy, M. A. (1995). Genetic exchange among linear replicons of the lyme disease spirochete, Borrelia burgdorferi;. (Masters Thesis). University of Utah. Retrieved from http://content.lib.utah.edu/cdm/singleitem/collection/etd1/id/543/rec/578
Chicago Manual of Style (16th Edition):
Murphy, Maria A. “Genetic exchange among linear replicons of the lyme disease spirochete, Borrelia burgdorferi;.” 1995. Masters Thesis, University of Utah. Accessed February 27, 2021.
http://content.lib.utah.edu/cdm/singleitem/collection/etd1/id/543/rec/578.
MLA Handbook (7th Edition):
Murphy, Maria A. “Genetic exchange among linear replicons of the lyme disease spirochete, Borrelia burgdorferi;.” 1995. Web. 27 Feb 2021.
Vancouver:
Murphy MA. Genetic exchange among linear replicons of the lyme disease spirochete, Borrelia burgdorferi;. [Internet] [Masters thesis]. University of Utah; 1995. [cited 2021 Feb 27].
Available from: http://content.lib.utah.edu/cdm/singleitem/collection/etd1/id/543/rec/578.
Council of Science Editors:
Murphy MA. Genetic exchange among linear replicons of the lyme disease spirochete, Borrelia burgdorferi;. [Masters Thesis]. University of Utah; 1995. Available from: http://content.lib.utah.edu/cdm/singleitem/collection/etd1/id/543/rec/578
Temple University
14.
Cutrera, Jason Lewis.
Insights into the Structure and Function of PrgW and its Conserved Cysteines.
Degree: PhD, 2014, Temple University
URL: http://digital.library.temple.edu/u?/p245801coll10,299645
► Microbiology and Immunology
Enterococcus faecalis is a Gram-positive bacterial species that is typically a member of the human gastrointestinal tract microbiota. However, E. faecalis is…
(more)
▼ Microbiology and Immunology
Enterococcus faecalis is a Gram-positive bacterial species that is typically a member of the human gastrointestinal tract microbiota. However, E. faecalis is also a nosocomial pathogen, which is involved in urinary tract infections, soft tissue infections and endocarditis. In recent times, the occurrence of antibiotic resistance has complicated the treatment of these infections. One of the major differences between commensal and pathogenic strains of E. faecalis is that pathogens contain multiple mobile elements such as plasmids, transposons and integrative conjugative elements (ICE). These elements allow for the acquisition and transfer of virulence factors and resistance genes. Conjugative plasmids are a class of plasmids present in E. faecalis whose transfer to host cells is induced by a small pheromone peptide, cCF10 (LVTLVFV). This peptide is initially encoded as a 22-amino acid precursor (pre-cCF10) from the signal sequence of the chromosomal ccfA gene and is then proteolytically cleaved by signal peptidase II and Eep. Once pCF10 has been transferred a host E. faecalis cell, it is exceptionally stable. A replicon clone is maintained in greater than 85% of host cells over 100 generations in the absence of selection, suggesting the stability of pCF10 is intrinsic to the replicon. Three unique features of the replication initiation protein PrgW may contribute to this stability: (a) the interaction of PrgW with pre-cCF10, (b) disulfide bond formation at three conserved cysteines (C78, C275, and C307) in PrgW, and (c) processing of the nascent PrgW protein. Replication initiation proteins associated with theta replicons, such as pCF10, are often self-contained units. To initiate plasmid replication, the replication initiation protein (PrgW) binds to direct repeats (oriV) in its own coding sequence (prgW). In silico analysis of PrgW suggests the existence of three distinct domains within the protein. The first 122 amino acids are homologous to a conserved domain present in related replication initiation proteins, which includes a Helix-Turn-Helix (HTH) DNA binding domain. This suggests that this domain of PrgW has a DNA-binding function and binds to the oriV site in prgW. The following 61 amino acids are not similar to any known sequence, and are encoded by the DNA sequence containing the direct repeats in the oriV site. This domain may or may not have a distinct function. The remaining sequence forms a domain that contains cysteines C275 and C307, and is also similar to no known structure. It is hypothesized that this domain is related to the stability of pCF10. C307 appears to be critical, as previous experiments indicate that its mutation alone affects plasmid stability. Secondary structure analysis of this domain revealed the presence of multiple alpha-helices that contain distinct hydrophobic domains that possibly contribute to pre-cCF10 binding and PrgW tertiary structure. The positions of the conserved cysteines within these alpha-helices may stabilize a hydrophobic binding…
Advisors/Committee Members: Buttaro, Bettina A.;, Tsygankov, Alexander, Tukel, Cagla, Monestier, Marc, Grubmeyer, Charles;.
Subjects/Keywords: Microbiology;
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APA (6th Edition):
Cutrera, J. L. (2014). Insights into the Structure and Function of PrgW and its Conserved Cysteines. (Doctoral Dissertation). Temple University. Retrieved from http://digital.library.temple.edu/u?/p245801coll10,299645
Chicago Manual of Style (16th Edition):
Cutrera, Jason Lewis. “Insights into the Structure and Function of PrgW and its Conserved Cysteines.” 2014. Doctoral Dissertation, Temple University. Accessed February 27, 2021.
http://digital.library.temple.edu/u?/p245801coll10,299645.
MLA Handbook (7th Edition):
Cutrera, Jason Lewis. “Insights into the Structure and Function of PrgW and its Conserved Cysteines.” 2014. Web. 27 Feb 2021.
Vancouver:
Cutrera JL. Insights into the Structure and Function of PrgW and its Conserved Cysteines. [Internet] [Doctoral dissertation]. Temple University; 2014. [cited 2021 Feb 27].
Available from: http://digital.library.temple.edu/u?/p245801coll10,299645.
Council of Science Editors:
Cutrera JL. Insights into the Structure and Function of PrgW and its Conserved Cysteines. [Doctoral Dissertation]. Temple University; 2014. Available from: http://digital.library.temple.edu/u?/p245801coll10,299645
Cornell University
15.
Moreno Switt, Andrea.
Genomic Characterization Of Salmonella Free-Living Phages, Plasmids And Chromosomally Inserted Mobile Elements.
Degree: PhD, Food Science and Technology, 2013, Cornell University
URL: http://hdl.handle.net/1813/33807
► Salmonella is a globally distributed foodborne pathogen. Historically, Salmonella have been associated with multiple foodborne outbreaks. In the United States, Salmonella cause an estimated of…
(more)
▼ Salmonella is a globally distributed foodborne pathogen. Historically, Salmonella have been associated with multiple foodborne outbreaks. In the United States, Salmonella cause an estimated of 1 million cases, and is reported as the foodborne pathogen that causes the highest number of deaths and hospitalizations. Salmonella is a very versatile pathogen, with two species, S. enterica and S. bongori, and more than 2,500 serovars. Our current understanding of Salmonella serovars have shown that mobile elements, such as phages,
plasmids and genomic islands have played a fundamental role in the evolution of this pathogen and in the emergence of endemic strains. Currently, sequencing technologies have improved dramatically, and its applications in the study of foodborne pathogens now just started being recognized. In this work we used next generation sequencing technologies to study a variety of Salmonella mobile elements, including free-living phages isolated from dairy farms, prophages inserted in the genome of several Salmonella serovars,
plasmids, and chromosomally inserted transposons and genomic islands. Salmonella phages were isolated from 15 dairy farms, and were subsequently phenotypically (host range) and genotypically (genome size) characterized. We found a high abundance and diversity of Salmonella phage on the farms; phage diversity was represented by narrow and wide host range phages, and by a number of genome sizes. To obtain a better knowledge of the isolated phages, we selected 22 phages for whole genome sequencing. These phages were classified into 9 different clusters, including three novel clusters that represent virulent and temperate life cycles. We also identified phage-borne antimicrobial resistance, virulence, and DNA metabolism genes; finally we obtained some insights related with phage host specificity. Finally, to gain a broader picture of Salmonella mobile elements, we sequenced the genomes of 16 Salmonella strains, representing different serovars; followed by a genome-scale prediction of mobile elements. We identified four novel IncI1-IncFIB cointegrated
plasmids, two resistance
plasmids in the same isolate of Salmonella Montevideo, 42 prophages, two novel genomic islands, and an ICE encoding the Typhi type IVb pilus operon. This study represents the first comprehensive study of the "Salmonella mobilome", which included free-living phages,
plasmids, and chromosomal elements.
Advisors/Committee Members: Wiedmann, Martin (chair), Peters, Joseph E. (committee member), Altier, Craig (committee member).
Subjects/Keywords: Salmonella; phages; plasmids; genomic islands; dairy farms
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APA (6th Edition):
Moreno Switt, A. (2013). Genomic Characterization Of Salmonella Free-Living Phages, Plasmids And Chromosomally Inserted Mobile Elements. (Doctoral Dissertation). Cornell University. Retrieved from http://hdl.handle.net/1813/33807
Chicago Manual of Style (16th Edition):
Moreno Switt, Andrea. “Genomic Characterization Of Salmonella Free-Living Phages, Plasmids And Chromosomally Inserted Mobile Elements.” 2013. Doctoral Dissertation, Cornell University. Accessed February 27, 2021.
http://hdl.handle.net/1813/33807.
MLA Handbook (7th Edition):
Moreno Switt, Andrea. “Genomic Characterization Of Salmonella Free-Living Phages, Plasmids And Chromosomally Inserted Mobile Elements.” 2013. Web. 27 Feb 2021.
Vancouver:
Moreno Switt A. Genomic Characterization Of Salmonella Free-Living Phages, Plasmids And Chromosomally Inserted Mobile Elements. [Internet] [Doctoral dissertation]. Cornell University; 2013. [cited 2021 Feb 27].
Available from: http://hdl.handle.net/1813/33807.
Council of Science Editors:
Moreno Switt A. Genomic Characterization Of Salmonella Free-Living Phages, Plasmids And Chromosomally Inserted Mobile Elements. [Doctoral Dissertation]. Cornell University; 2013. Available from: http://hdl.handle.net/1813/33807
Stellenbosch University
16.
Gardner, Murray Newell.
An investigation into the replicon of a broad host range mobilizable plasmid from the moderately thermophilic bacterium Acidithiobacillus caldus.
Degree: Microbiology, 2003, Stellenbosch University
URL: http://hdl.handle.net/10019.1/53273
► Dissertation (PhD) – University of Stellenbosch, 2003.
ENGLISH ABSTRACT: The moderately thermophilic (45 to 50DC), highly acidophilic (pH 1.5 to 2.5), chemolithoautotrophic Acidithiobaci/lus caldus strain "f'…
(more)
▼ Dissertation (PhD) – University of Stellenbosch, 2003.
ENGLISH ABSTRACT: The moderately thermophilic (45 to 50DC), highly acidophilic (pH 1.5 to 2.5),
chemolithoautotrophic Acidithiobaci/lus caldus strain "f' was isolated from a
biooxidation process used to treat nickel ore concentrates. Trans-Alternating Field
Electrophoresis (TAFE) analysis of total DNA from the At. caldus cells revealed two
plasmids of approximately 14 and 45-kb. The 14-kb plasmid, designated pTC-F14,
was cloned and shown by replacement of the cloning vector with a kanamycin
resistance gene to be capable of autonomous replication in Escherichia coli.
Autonomous replication was also demonstrated in Pseudomonas putida and
Agrobacterium tumefaciens LBA 4404 which suggested that pTC-F14 was a broad
host-range plasmid. Sequence analysis of the pTC-F14 replicon region revealed five
open-reading frames, and a replicon organization like that of the broad host-range
IncQ plasmids. Three of the open-reading frames encoded replication proteins with
amino acid sequence identities similar to that of the IncQ-like plasmid pTF-FC2
(RepA, 81%; RepB, 78%; RepC, 74%). This high level of relatedness suggested that
the two replicons had evolved from a common ancestor. Since closely related
replicons are usually incompatible, the compatible replicons of pTC-F14 and pTFFC2
raised the question of how the replicons of the two sister plasmids had evolved
such that they can now co-exist in the same host cell line. Further incompatibility
testing with the IncQ-like plasmid pIEll08 and the IncQ prototype plasmid
RSF10101R11621R300B determined that pTC-F14 was compatible with pIEI108, but
incompatible with the IncQ prototype plasmid. It was found that the RepB and RepA
replication proteins ofpTF-FC2 and pIEll08 were able to complement the pTC-FI4
orthologs only ifpTC-F14 RepC was present in trans. The RepC protein ofpTC-F14
was thus plasmid-template specific, while the RepA and RepB proteins were less
plasmid-template specific. A five nucleotide possible iteron-discriminating region in
the direct repeats of IncQ-like plasmid oriV regions has been identified (Tietze, E.
(1998) Plasmid 39: 165-181). The iteron sequence ofpTC-F14 differs from pTF-FC2
and pIE 1108 by three nucleotides in this iteron-discriminating region. It was
therefore proposed that co-evolution of the iterons and the RepC protein to a point
where the RepC protein no longer recognizes the iteron sequence of a closely related
sister plasmid is the mechanism by which replicons evolve to become compatible in the same host cell. The incompatibility determinant of the IncQ prototype plasmid
RSFlOlOIR11621R300B was also sought, and subsequently localized to the region
encoding the IncQ prototype plasmid's repAC genes. Interference with the initiation
of pTC-F14 replication by the IncQ prototype plasmid was demonstrated by growth
inhibition of a replication-deficient M13 bacteriophage into which oriVpTC-F14 had
been cloned. Secondly, the IncQ prototype derivative pKE462 displaced…
Advisors/Committee Members: Rawlings, D. E., Stellenbosch University. Faculty of Science. Dept. of Microbiology..
Subjects/Keywords: Thermophilic bacteria; Plasmids
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APA (6th Edition):
Gardner, M. N. (2003). An investigation into the replicon of a broad host range mobilizable plasmid from the moderately thermophilic bacterium Acidithiobacillus caldus. (Thesis). Stellenbosch University. Retrieved from http://hdl.handle.net/10019.1/53273
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Gardner, Murray Newell. “An investigation into the replicon of a broad host range mobilizable plasmid from the moderately thermophilic bacterium Acidithiobacillus caldus.” 2003. Thesis, Stellenbosch University. Accessed February 27, 2021.
http://hdl.handle.net/10019.1/53273.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Gardner, Murray Newell. “An investigation into the replicon of a broad host range mobilizable plasmid from the moderately thermophilic bacterium Acidithiobacillus caldus.” 2003. Web. 27 Feb 2021.
Vancouver:
Gardner MN. An investigation into the replicon of a broad host range mobilizable plasmid from the moderately thermophilic bacterium Acidithiobacillus caldus. [Internet] [Thesis]. Stellenbosch University; 2003. [cited 2021 Feb 27].
Available from: http://hdl.handle.net/10019.1/53273.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Gardner MN. An investigation into the replicon of a broad host range mobilizable plasmid from the moderately thermophilic bacterium Acidithiobacillus caldus. [Thesis]. Stellenbosch University; 2003. Available from: http://hdl.handle.net/10019.1/53273
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Iowa State University
17.
Naditz, Annabel.
A comparative analysis of Listeria monocytogenes plasmids: Presence, contribution to stress and conservation.
Degree: 2020, Iowa State University
URL: https://lib.dr.iastate.edu/etd/17859
► Listeria monocytogenes is a food-borne pathogen responsible for the disease listeriosis and is commonly isolated from food products and production facilities. Some of these isolates…
(more)
▼ Listeria monocytogenes is a food-borne pathogen responsible for the disease listeriosis and is commonly isolated from food products and production facilities. Some of these isolates are known to contain plasmids, though previous plasmid studies were conducted with a limited data set of 14 sequenced strains. To determine the presence of plasmids in a variety of L. monocytogenes strains and from various sequence types (ST), a large-scale analysis of 1924 L. monocytogenes genome sequences was conducted to determine the presence of plasmids in different STs, years, countries, and isolation sources. On average, 53% of all L. monocytogenes strains, and up to 92% of the strains within a ST (i.e. ST121), analyzed contained a plasmid. These plasmids ranged in size between 3 kb to 140 kb, with the majority being around 58 kb (± 1kb) and the other around 77 kb (± 1 kb). Plasmid conservation was a minimum of 91.9% nucleotide identity across all plasmids among all STs and the average similarity was 92.3% similarity. Examination of six previously identified plasmid-encoded stress-related genes indicated the genes present in L. monocytogenes plasmids are highly conserved, but their presence in plasmids is highly variable. Analysis of the dataset also identified a novel mercury-resistance operon containing seven genes involved in increasing tolerance in the presence of mercury.
Understanding that plasmids often play a role in stress survival, it was necessary to determine the contribution of L. monocytogenes plasmids to stress tolerance when under such conditions. For this aim, we studied three isogenic pairs of L. monocytogenes ST5, ST8, and ST121 strains, isolated from dairy foods and production facilities, and that either contained or were cured of plasmids harboring putative stress response genes. Wildtype (wt) and plasmid-cured (pc) strains were exposed to sublethal concentrations of oxidative stress (0.01% H2O2), salinity (15% wt/vol NaCl, pH 5), acidity (1% v/v lactic acid, pH 3.4), heat stress, or disinfectant (benzalkonium chloride, BC). When exposed to H2O2, NaCl, or lactic acid, CFU counts for all wt strains were significantly higher (P < 0.05) than those of the pc strains. Our data revealed that L. monocytogenes ST5, ST8, and ST121 plasmids confer increased tolerance against elevated temperature, salinity, acidic environments, oxidative stress and disinfectants.
Further examination of the phenotypic and functional characteristics of plasmids was required to better understand the mechanisms used by L. monocytogenes plasmids to confer tolerance to stress conditions. Therefore, a transcriptomics survey of plasmid-carrying L. monocytogenes strains 6179 and R479a was conducted under acidic and oxidative stress conditions, using the acidic and oxidative conditions of functional characterization, with an exposure time of 30 minutes at 20°C. The data showed no significant gene expression in the oxidative stress conditions for the R479a strain, and a single differentially expressed (DE) gene in the 6179 strain, a protein of…
Subjects/Keywords: Listeria monocytogenes; Plasmids; Stress; Survey; Transcriptomics
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APA (6th Edition):
Naditz, A. (2020). A comparative analysis of Listeria monocytogenes plasmids: Presence, contribution to stress and conservation. (Thesis). Iowa State University. Retrieved from https://lib.dr.iastate.edu/etd/17859
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Naditz, Annabel. “A comparative analysis of Listeria monocytogenes plasmids: Presence, contribution to stress and conservation.” 2020. Thesis, Iowa State University. Accessed February 27, 2021.
https://lib.dr.iastate.edu/etd/17859.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Naditz, Annabel. “A comparative analysis of Listeria monocytogenes plasmids: Presence, contribution to stress and conservation.” 2020. Web. 27 Feb 2021.
Vancouver:
Naditz A. A comparative analysis of Listeria monocytogenes plasmids: Presence, contribution to stress and conservation. [Internet] [Thesis]. Iowa State University; 2020. [cited 2021 Feb 27].
Available from: https://lib.dr.iastate.edu/etd/17859.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Naditz A. A comparative analysis of Listeria monocytogenes plasmids: Presence, contribution to stress and conservation. [Thesis]. Iowa State University; 2020. Available from: https://lib.dr.iastate.edu/etd/17859
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
University of Cape Town
18.
Tatley, Fernanda Maria Palma Ribeiro da Silva.
Characterisation of a replicon of the conjugative, multiple drug resistance, moderately promiscuous, plasmid pGSH500.
Degree: Image, Division of Medical Microbiology, 1992, University of Cape Town
URL: http://hdl.handle.net/11427/25668
Subjects/Keywords: Plasmids - analysis; Replicon
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APA (6th Edition):
Tatley, F. M. P. R. d. S. (1992). Characterisation of a replicon of the conjugative, multiple drug resistance, moderately promiscuous, plasmid pGSH500. (Thesis). University of Cape Town. Retrieved from http://hdl.handle.net/11427/25668
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Tatley, Fernanda Maria Palma Ribeiro da Silva. “Characterisation of a replicon of the conjugative, multiple drug resistance, moderately promiscuous, plasmid pGSH500.” 1992. Thesis, University of Cape Town. Accessed February 27, 2021.
http://hdl.handle.net/11427/25668.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Tatley, Fernanda Maria Palma Ribeiro da Silva. “Characterisation of a replicon of the conjugative, multiple drug resistance, moderately promiscuous, plasmid pGSH500.” 1992. Web. 27 Feb 2021.
Vancouver:
Tatley FMPRdS. Characterisation of a replicon of the conjugative, multiple drug resistance, moderately promiscuous, plasmid pGSH500. [Internet] [Thesis]. University of Cape Town; 1992. [cited 2021 Feb 27].
Available from: http://hdl.handle.net/11427/25668.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Tatley FMPRdS. Characterisation of a replicon of the conjugative, multiple drug resistance, moderately promiscuous, plasmid pGSH500. [Thesis]. University of Cape Town; 1992. Available from: http://hdl.handle.net/11427/25668
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Northeastern University
19.
Avachat, Charul.
In vitro evaluation of cationic liposomal delivery of BDNF expressing plasmid DNA in experimental model of Parkinson's disease.
Degree: MS, School of Pharmacy, 2017, Northeastern University
URL: http://hdl.handle.net/2047/D20261856
► With the advent of gene therapy it has now become possible to target specific genes which have been either upregulated or down regulated in neurodegenerative…
(more)
▼ With the advent of gene therapy it has now become possible to target specific genes which have been either upregulated or down regulated in neurodegenerative disorders. Brain-derived neurotrophic factor (BDNF) belongs to the family of neurotrophins and has shown significant activity in protecting dopaminergic neurons and even promoting growth and differentiation. It has shown to inhibit apoptosis mediated cell death and neurodegeneration induced by neurotoxins. Studies have indicated that BDNF is expressed by dopaminergic neurons in the substantia nigra and ventral tegmental area. Low level of BDNF mRNA expression has been associated with depletion of dopaminergic neurons. Also BDNF has shown to improve cognition in patients with PD. The main delivery challenge is that BDNF does not cross the BBB easily, owing to its high molecular weight and low diffusion across the brain parenchyma. Thus effective strategies are required for the delivery of this molecule.; Plasmids can be used as gene delivery vectors due to its high stability as compared to other nucleic acid molecules. These plasmids when protected with the help of lipid moieties further enhances the stability of the delivery system. Cationic liposomes serve as efficient carriers since they are easily taken up by the brain as compared to anionic and neutral liposomes. Plasmid was selected as a therapeutic agent since it offers several advantages over protein therapeutics like: better invivo and invitro stability, low immunogenecity and longer half life.; This MS dissertation project focuses on the formulation of a cationic liposomal drug delivery system encapsulating Plasmid DNA capable of expressing BDNF and delivering it to mammalian cells to evaluate the transfection efficiency of the liposomes and also assess the upregulation in BDNF mRNA levels. Further it also examines the potential of BDNF plasmid to protect the cells from neurodegeneration in an invitro PD cell model.
Subjects/Keywords: Parkinson's disease; brain-derived neurotrophic factor; plasmids
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APA (6th Edition):
Avachat, C. (2017). In vitro evaluation of cationic liposomal delivery of BDNF expressing plasmid DNA in experimental model of Parkinson's disease. (Masters Thesis). Northeastern University. Retrieved from http://hdl.handle.net/2047/D20261856
Chicago Manual of Style (16th Edition):
Avachat, Charul. “In vitro evaluation of cationic liposomal delivery of BDNF expressing plasmid DNA in experimental model of Parkinson's disease.” 2017. Masters Thesis, Northeastern University. Accessed February 27, 2021.
http://hdl.handle.net/2047/D20261856.
MLA Handbook (7th Edition):
Avachat, Charul. “In vitro evaluation of cationic liposomal delivery of BDNF expressing plasmid DNA in experimental model of Parkinson's disease.” 2017. Web. 27 Feb 2021.
Vancouver:
Avachat C. In vitro evaluation of cationic liposomal delivery of BDNF expressing plasmid DNA in experimental model of Parkinson's disease. [Internet] [Masters thesis]. Northeastern University; 2017. [cited 2021 Feb 27].
Available from: http://hdl.handle.net/2047/D20261856.
Council of Science Editors:
Avachat C. In vitro evaluation of cationic liposomal delivery of BDNF expressing plasmid DNA in experimental model of Parkinson's disease. [Masters Thesis]. Northeastern University; 2017. Available from: http://hdl.handle.net/2047/D20261856
Central Connecticut State University
20.
Oppedisano, Michelle A.
The purification of the Shigella flexneri virulence plasmid.
Degree: Department of Biological Sciences, 2002, Central Connecticut State University
URL: http://content.library.ccsu.edu/u?/ccsutheses,1293
► The intent of this research project was to purify the Shigella flexneri virulence plasmid without the use of ultra-centrifugation. The ultra-centrifugation is the most commonly…
(more)
▼ The intent of this research project was to purify the Shigella flexneri virulence plasmid without the use of ultra-centrifugation. The ultra-centrifugation is the most commonly used technique; however the PFGE has proven to isolate the plasmid with the similar efficiency without decreasing quality of the DNA sample. Once the plasmid was purified, the construction of a library of the plasmid was started. A library of this particular bacterial plasmid will help greatly in identifying the genes responsible for the maintenance of this plasmid. This particular plasmid is of great interest because it is primarily responsible for the pathogenic characteristics of the bacteria. The S. flexneri virulence plasmid is a highly stable single copy plasmid that is approximately 230kb in size. The stability of the plasmid is essential for the bacteria's ability to cause disease. The identification of the stability genes encoded in the Shigella flexneri virulence plasmid would greatly increase our ability to identify a possibly treatment for shigellosis infections. The project's first goal was to successfully isolate the large virulence plasmid from the chromosomal DNA using the technique of Pulse Field Gel Electrophoresis. This gel apparatus allows much larger fragments of DNA to be separated in comparison to the standard agarose gels. In addition the sample preparation involves decreased risk of shearing the large DNA in the sample. The second goal was to create a library of the plasmid, using the appropriate enzymes and test
plasmids. Three restriction enzymes were chosen to insure that the targeted stability genes would be intact within the library. The test
plasmids were also chosen carefully to insure the later selection of the stability genes would be possible.
Advisors/Committee Members: Davis, Michael.
Subjects/Keywords: Shigella flexneri; Plasmids
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APA (6th Edition):
Oppedisano, M. A. (2002). The purification of the Shigella flexneri virulence plasmid. (Thesis). Central Connecticut State University. Retrieved from http://content.library.ccsu.edu/u?/ccsutheses,1293
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Oppedisano, Michelle A. “The purification of the Shigella flexneri virulence plasmid.” 2002. Thesis, Central Connecticut State University. Accessed February 27, 2021.
http://content.library.ccsu.edu/u?/ccsutheses,1293.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Oppedisano, Michelle A. “The purification of the Shigella flexneri virulence plasmid.” 2002. Web. 27 Feb 2021.
Vancouver:
Oppedisano MA. The purification of the Shigella flexneri virulence plasmid. [Internet] [Thesis]. Central Connecticut State University; 2002. [cited 2021 Feb 27].
Available from: http://content.library.ccsu.edu/u?/ccsutheses,1293.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Oppedisano MA. The purification of the Shigella flexneri virulence plasmid. [Thesis]. Central Connecticut State University; 2002. Available from: http://content.library.ccsu.edu/u?/ccsutheses,1293
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Universidad de Cantabria
21.
Ruiz Cantos, Miriam.
Mobilization of the ColE1 Plasmid with Synthetic Constructs.
Degree: Máster en Biología Molecular y Biomedicina, 2016, Universidad de Cantabria
URL: http://hdl.handle.net/10902/9021
Subjects/Keywords: Mobilization of plasmids
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APA (6th Edition):
Ruiz Cantos, M. (2016). Mobilization of the ColE1 Plasmid with Synthetic Constructs. (Masters Thesis). Universidad de Cantabria. Retrieved from http://hdl.handle.net/10902/9021
Chicago Manual of Style (16th Edition):
Ruiz Cantos, Miriam. “Mobilization of the ColE1 Plasmid with Synthetic Constructs.” 2016. Masters Thesis, Universidad de Cantabria. Accessed February 27, 2021.
http://hdl.handle.net/10902/9021.
MLA Handbook (7th Edition):
Ruiz Cantos, Miriam. “Mobilization of the ColE1 Plasmid with Synthetic Constructs.” 2016. Web. 27 Feb 2021.
Vancouver:
Ruiz Cantos M. Mobilization of the ColE1 Plasmid with Synthetic Constructs. [Internet] [Masters thesis]. Universidad de Cantabria; 2016. [cited 2021 Feb 27].
Available from: http://hdl.handle.net/10902/9021.
Council of Science Editors:
Ruiz Cantos M. Mobilization of the ColE1 Plasmid with Synthetic Constructs. [Masters Thesis]. Universidad de Cantabria; 2016. Available from: http://hdl.handle.net/10902/9021
Georgia Tech
22.
Scott, David Lee Jr.
Identification of a region in the central regulatory segment of plasmid R6K responsible for complexing to membranes of escherichia coli.
Degree: MS, Biology, 1994, Georgia Tech
URL: http://hdl.handle.net/1853/31050
Subjects/Keywords: Plasmids; Escherichia coli
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APA (6th Edition):
Scott, D. L. J. (1994). Identification of a region in the central regulatory segment of plasmid R6K responsible for complexing to membranes of escherichia coli. (Masters Thesis). Georgia Tech. Retrieved from http://hdl.handle.net/1853/31050
Chicago Manual of Style (16th Edition):
Scott, David Lee Jr. “Identification of a region in the central regulatory segment of plasmid R6K responsible for complexing to membranes of escherichia coli.” 1994. Masters Thesis, Georgia Tech. Accessed February 27, 2021.
http://hdl.handle.net/1853/31050.
MLA Handbook (7th Edition):
Scott, David Lee Jr. “Identification of a region in the central regulatory segment of plasmid R6K responsible for complexing to membranes of escherichia coli.” 1994. Web. 27 Feb 2021.
Vancouver:
Scott DLJ. Identification of a region in the central regulatory segment of plasmid R6K responsible for complexing to membranes of escherichia coli. [Internet] [Masters thesis]. Georgia Tech; 1994. [cited 2021 Feb 27].
Available from: http://hdl.handle.net/1853/31050.
Council of Science Editors:
Scott DLJ. Identification of a region in the central regulatory segment of plasmid R6K responsible for complexing to membranes of escherichia coli. [Masters Thesis]. Georgia Tech; 1994. Available from: http://hdl.handle.net/1853/31050
University of Waterloo
23.
Thomas, Janis.
Distribution, diversity and antimicrobial resistance of Salmonella enterica isolated from urban and rural streams.
Degree: 2011, University of Waterloo
URL: http://hdl.handle.net/10012/6071
► This study presents the spatial and temporal variability of Salmonella enterica in urban and rural streams in a model watershed (Grand River watershed, Ontario, Canada),…
(more)
▼ This study presents the spatial and temporal variability of Salmonella enterica in urban and rural streams in a model watershed (Grand River watershed, Ontario, Canada), and examines the antimicrobial resistance (AMR) and genetic diversity of various serotypes. Using a swab collection method and various media types, Salmonella were detected in 78.4% of samples between November 2003 and July 2005. A diverse range of Salmonella serotypes (n=38) were isolated from water. Predominant serotypes and phagetypes (PT), including S. Typhimurium PT 104 and S. Heidelberg PT 19, and the proportion of isolates demonstrating AMR (33%), was similar to those for humans and farm animals locally and across Canada, a trend not commonly reported. There was a greater diversity of serotypes and AMR profiles in isolates from the urban stream compared to the rural/agricultural streams. Plasmid-borne resistance was observed in 28.6% of AMR isolates, with two different plasmids responsible for resistance; the TEM-1 plasmid (8.1Kb plasmids carrying blaTEM-1, responsible for ampicillin resistance) and CMY-2 plasmid (95.5Kb plasmids carrying blaCMY-2, responsible for 3rd generation cephalosporin resistance). CMY-2 plasmids were only found in the urban stream and did not create a biological burden under non-selective conditions, indicating the long-term permanence of these plasmids. Seasonal differences in the overall diversity of serotypes and predominance of serotypes of human health significance (S. Typhimurium and S. Heidelberg) were observed. The lower occurrence of S. Typhimurium and S. Heidelberg in February and March was not the result of lower survival of these serotypes at low temperatures. Peaks in occurrence of S. Typhimurium and S. Heidelberg in the summer and spring, respectively, were pronounced in the rural/agricultural streams, as opposed to the urban stream. Pulsed-field gel electrophoresis and plasmid-typing revealed diversity within multiple drug resistant S. Typhimurium PT 104 isolates, indicating genetic differences among tributaries. The ubiquitous nature of Salmonella in water and the predominance of serotypes/phagetypes of human or veterinary health significance suggest that environmental exposure through consumption or contact with contaminated water is plausible. These streams may act as a vehicle for the dissemination of these organisms and their resistance genes between different hosts or environments.
Subjects/Keywords: Salmonella; antimicrobial resistance; water; plasmids; waterborne pathogens
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APA (6th Edition):
Thomas, J. (2011). Distribution, diversity and antimicrobial resistance of Salmonella enterica isolated from urban and rural streams. (Thesis). University of Waterloo. Retrieved from http://hdl.handle.net/10012/6071
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Thomas, Janis. “Distribution, diversity and antimicrobial resistance of Salmonella enterica isolated from urban and rural streams.” 2011. Thesis, University of Waterloo. Accessed February 27, 2021.
http://hdl.handle.net/10012/6071.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Thomas, Janis. “Distribution, diversity and antimicrobial resistance of Salmonella enterica isolated from urban and rural streams.” 2011. Web. 27 Feb 2021.
Vancouver:
Thomas J. Distribution, diversity and antimicrobial resistance of Salmonella enterica isolated from urban and rural streams. [Internet] [Thesis]. University of Waterloo; 2011. [cited 2021 Feb 27].
Available from: http://hdl.handle.net/10012/6071.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Thomas J. Distribution, diversity and antimicrobial resistance of Salmonella enterica isolated from urban and rural streams. [Thesis]. University of Waterloo; 2011. Available from: http://hdl.handle.net/10012/6071
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
24.
Asghari, Abdolkarim.
Physical characterization and restriction mapping of the SAL plasmid from Pseudomonas putida.
Degree: 1987, North Texas State University
URL: https://digital.library.unt.edu/ark:/67531/metadc798475/
► Physical and restriction mapping of the salicylate catabolic plasmid SAL from Pseudomonas putida strain PpG 2119 was carried out by standard multiple restriction analysis and…
(more)
▼ Physical and restriction mapping of the salicylate catabolic plasmid SAL from Pseudomonas putida strain PpG 2119 was carried out by standard multiple restriction analysis and by cross hybridization studies using radioactively labeled restriction fragment probes. The total numbers of fragments produced, their respective sizes, the arrangement of the restriction fragments on the plasmid and the map locations of the enzyme recognition sites for Hpal, Xhol, Dral and Smal are given.
Subjects/Keywords: pseudomonas; plasmids; biology
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25.
Verweij, Wilco.
Coexistence between Plasmids in Structured Bioflms: A Theoretical Model for Post-Segregational Killing Systems.
Degree: MS, Department of Integrative Biology, 2019, University of Guelph
URL: https://atrium.lib.uoguelph.ca/xmlui/handle/10214/16264
► Currently there is a lack of understanding about how plasmids that contain Post-Segregational Killing (PSK+) systems and plasmids that do not contain such a system…
(more)
▼ Currently there is a lack of understanding about how
plasmids that contain Post-Segregational Killing
(PSK+) systems and
plasmids that do not contain such a system coexist in a population. In this thesis
two spatially explicate models are presented to attempt to answer this gap in the literature. The rst
model is an extension on a previous single strain model, the second model has two di erent strains and
allows for the
plasmids to specialize to di erent bacterial strains. Weak coexistence was found in the single
strain model. Coexistence was found in the second model but only across a small parameter space and only
under conditions when a PSK+ plasmid is introduce by a process analogous to dispersal. The interesting
implication of this result is that it suggests coexistence is possible with specialization, but that it requires
dispersal from another locality for the coexistence process to be initiated.
Advisors/Committee Members: Griswold, Cortland (advisor).
Subjects/Keywords: Post Segregational Killing Systems; Plasmids; Theoretical Biology
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APA (6th Edition):
Verweij, W. (2019). Coexistence between Plasmids in Structured Bioflms: A Theoretical Model for Post-Segregational Killing Systems. (Masters Thesis). University of Guelph. Retrieved from https://atrium.lib.uoguelph.ca/xmlui/handle/10214/16264
Chicago Manual of Style (16th Edition):
Verweij, Wilco. “Coexistence between Plasmids in Structured Bioflms: A Theoretical Model for Post-Segregational Killing Systems.” 2019. Masters Thesis, University of Guelph. Accessed February 27, 2021.
https://atrium.lib.uoguelph.ca/xmlui/handle/10214/16264.
MLA Handbook (7th Edition):
Verweij, Wilco. “Coexistence between Plasmids in Structured Bioflms: A Theoretical Model for Post-Segregational Killing Systems.” 2019. Web. 27 Feb 2021.
Vancouver:
Verweij W. Coexistence between Plasmids in Structured Bioflms: A Theoretical Model for Post-Segregational Killing Systems. [Internet] [Masters thesis]. University of Guelph; 2019. [cited 2021 Feb 27].
Available from: https://atrium.lib.uoguelph.ca/xmlui/handle/10214/16264.
Council of Science Editors:
Verweij W. Coexistence between Plasmids in Structured Bioflms: A Theoretical Model for Post-Segregational Killing Systems. [Masters Thesis]. University of Guelph; 2019. Available from: https://atrium.lib.uoguelph.ca/xmlui/handle/10214/16264
Massey University
26.
Ward, Lawrence James Henry.
Plasmids in Rhizobium phaseoli.
Degree: MS, Microbiology, 1982, Massey University
URL: http://hdl.handle.net/10179/6547
► Fast growing strains of Rhizobium have been divided into four homology groups on the basis of DNA hybridization. Rhizobia from two of these homology groups…
(more)
▼ Fast growing strains of Rhizobium have been divided into four homology groups on the basis of DNA hybridization. Rhizobia from two of these homology groups can form effective nodules with beans (Phaseolus vulgaris). Large plasmids associated with nodulation have been demonstrated in Rhizobium sp. A study was undertaken to examine the plasmids in rhizobia from the two different homology groups capable of nodulating beans. The effectiveness of strains of Rhizobium phaseoli on bean plants were examined. Spontaneous antibiotic resistant mutants which retained the ability to nodulate beans were selected. Antibiotic resistance marked clones were incubated at elevated temperatures to produce an ineffective mutant stain of Rhizobium phaseoli NZP 5492. Methods of extracting large plasmids from Rhizobium phaseoli were developed. Plasmids were visualised by agarose gel electrophoresis and purified by cesium chloride - ethidium bromide density gradient ultracentrifugation. We demonstrated the presence of plasmids of molecular weight range 66 Md to 316 Md in Rhizobium phaseoli strains. Some strains contained a single plasmid while others contained multiple plasmids. Comparisons were made between whole plasmids and restriction endonuclease digests of the plasmids from the two groups. The fragment pattern obtained from Eco RI digests showed differences in fragment numbers and size between plasmids from DNA homology group 1 and DNA homology group 2. Further studies using gel blotting and hybridization techniques are required to ascertain the degree of homology between the plasmids, both within groups and between groups. Rhizobium phaseoli NZP 5479 and NZP 5547 a non-nodulating mutant of strain NZP 5479 were examined. Both strains had plasmids of estimated molecular weights 186 Md and 288 Md. There was no detectable difference in the size of the plasmids in the non-nodulating mutant compared to the effective parent strain. Rhizobium phaseoli NZP 5492 B5/8 (effective) and NZP 5492 B5/1 an ineffective mutant obtained from strain NZP 5492 had plasmids of similar molecular weight. Differences were observed in the Eco RI fragment pattern and possible rearrangements to the DNA to account for these differences are presented.
Subjects/Keywords: Plasmids;
Rhizobium phaseoli
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APA (6th Edition):
Ward, L. J. H. (1982). Plasmids in Rhizobium phaseoli. (Masters Thesis). Massey University. Retrieved from http://hdl.handle.net/10179/6547
Chicago Manual of Style (16th Edition):
Ward, Lawrence James Henry. “Plasmids in Rhizobium phaseoli.” 1982. Masters Thesis, Massey University. Accessed February 27, 2021.
http://hdl.handle.net/10179/6547.
MLA Handbook (7th Edition):
Ward, Lawrence James Henry. “Plasmids in Rhizobium phaseoli.” 1982. Web. 27 Feb 2021.
Vancouver:
Ward LJH. Plasmids in Rhizobium phaseoli. [Internet] [Masters thesis]. Massey University; 1982. [cited 2021 Feb 27].
Available from: http://hdl.handle.net/10179/6547.
Council of Science Editors:
Ward LJH. Plasmids in Rhizobium phaseoli. [Masters Thesis]. Massey University; 1982. Available from: http://hdl.handle.net/10179/6547
University of Texas – Austin
27.
Yang, Xianmei.
The stability system of the yeast 2 micron plasmid: analysis of plasmid and host encoded components.
Degree: PhD, Microbiology, 2002, University of Texas – Austin
URL: http://hdl.handle.net/2152/1071
► The work presented in this thesis aims to understand the molecular strategies used by an extrachromosomal selfish DNA element for its stable, high copy persistence.…
(more)
▼ The work presented in this thesis aims to understand the molecular
strategies used by an extrachromosomal selfish DNA element for its stable, high
copy persistence. The model system studied here is the 2 micron plasmid found
nearly ubiquitously in Saccharomyces yeast. By a combination of mutational and
functional analysis of a plasmid coded protein Rep1p, an essential component of
the stability system, we have provided support for the DNA-protein and proteinprotein
interactions predicted to be important in plasmid maintenance. Using cell
biological and molecular genetic methods, we have unveiled an apparent coupling
of the pathways for plasmid and chromosome segregation. Mutations that affect
equal partitioning of the chromosomes also affect the plasmid, and the two tend to
missegregate in tandem. We have identified host factors that interact with
components of the plasmid stability system, and may thus play a potential role in
plasmid partitioning. In particular, we have found that the yeast cohesin complex,
that bridges sister chromatids until they are ready to be unpaired and distributed to
the daughter cells arising from a division event, may serve an analogous function
in plasmid segregation. Our preliminary results suggest that the plasmid stability
system follows the ‘recruitment model’, in which a functional complex is
assembled by the sum of different sets of DNA-protein and protein-protein
interactions. It is possible to reconstitute an active partitioning complex through
an alternative set of interactions.
Advisors/Committee Members: Jayaram, Makkuni (advisor).
Subjects/Keywords: Saccharomyces – Genetics; Plasmids
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APA (6th Edition):
Yang, X. (2002). The stability system of the yeast 2 micron plasmid: analysis of plasmid and host encoded components. (Doctoral Dissertation). University of Texas – Austin. Retrieved from http://hdl.handle.net/2152/1071
Chicago Manual of Style (16th Edition):
Yang, Xianmei. “The stability system of the yeast 2 micron plasmid: analysis of plasmid and host encoded components.” 2002. Doctoral Dissertation, University of Texas – Austin. Accessed February 27, 2021.
http://hdl.handle.net/2152/1071.
MLA Handbook (7th Edition):
Yang, Xianmei. “The stability system of the yeast 2 micron plasmid: analysis of plasmid and host encoded components.” 2002. Web. 27 Feb 2021.
Vancouver:
Yang X. The stability system of the yeast 2 micron plasmid: analysis of plasmid and host encoded components. [Internet] [Doctoral dissertation]. University of Texas – Austin; 2002. [cited 2021 Feb 27].
Available from: http://hdl.handle.net/2152/1071.
Council of Science Editors:
Yang X. The stability system of the yeast 2 micron plasmid: analysis of plasmid and host encoded components. [Doctoral Dissertation]. University of Texas – Austin; 2002. Available from: http://hdl.handle.net/2152/1071
Michigan State University
28.
Vincent, Jeffrey Robert.
The transfer capabilities, maintenance and chromosomal integration of the IncP-1 plasmid pRD1 in Pseudomonas syringae pv. syringae PSSD220.
Degree: PhD, Department of Botany and Plant Pathology, 1985, Michigan State University
URL: http://etd.lib.msu.edu/islandora/object/etd:37678
Subjects/Keywords: Plasmids; Bacterial genetics
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APA (6th Edition):
Vincent, J. R. (1985). The transfer capabilities, maintenance and chromosomal integration of the IncP-1 plasmid pRD1 in Pseudomonas syringae pv. syringae PSSD220. (Doctoral Dissertation). Michigan State University. Retrieved from http://etd.lib.msu.edu/islandora/object/etd:37678
Chicago Manual of Style (16th Edition):
Vincent, Jeffrey Robert. “The transfer capabilities, maintenance and chromosomal integration of the IncP-1 plasmid pRD1 in Pseudomonas syringae pv. syringae PSSD220.” 1985. Doctoral Dissertation, Michigan State University. Accessed February 27, 2021.
http://etd.lib.msu.edu/islandora/object/etd:37678.
MLA Handbook (7th Edition):
Vincent, Jeffrey Robert. “The transfer capabilities, maintenance and chromosomal integration of the IncP-1 plasmid pRD1 in Pseudomonas syringae pv. syringae PSSD220.” 1985. Web. 27 Feb 2021.
Vancouver:
Vincent JR. The transfer capabilities, maintenance and chromosomal integration of the IncP-1 plasmid pRD1 in Pseudomonas syringae pv. syringae PSSD220. [Internet] [Doctoral dissertation]. Michigan State University; 1985. [cited 2021 Feb 27].
Available from: http://etd.lib.msu.edu/islandora/object/etd:37678.
Council of Science Editors:
Vincent JR. The transfer capabilities, maintenance and chromosomal integration of the IncP-1 plasmid pRD1 in Pseudomonas syringae pv. syringae PSSD220. [Doctoral Dissertation]. Michigan State University; 1985. Available from: http://etd.lib.msu.edu/islandora/object/etd:37678
University of Arizona
29.
DUBEL, JACQUELINE ROBERTA.
MOLECULAR AND GENETIC CHARACTERIZATION OF THE VIRULENCE PLASMID OF YERSINIA ENTEROCOLITICA.
Degree: 1983, University of Arizona
URL: http://hdl.handle.net/10150/187348
► Purified DNA from a nalidixic acid resistant derivative of a virulent serotype 0:3 clinical isolate of Yersinia enterocolitica was subjected to transpositional mutagenesis in an…
(more)
▼ Purified DNA from a nalidixic acid resistant derivative of a virulent serotype 0:3 clinical isolate of Yersinia enterocolitica was subjected to transpositional mutagenesis in an effort to construct avirulent mutants. The resulting transpositional mutants, as well as the wild-type virulent strain and its isogenic derivative that had been cured of the virulence plasmid, were analyzed for plasmid DNA content. The plasmid DNA content of each strain was further characterized by restriction endonuclease digestion and the transposon insertion sites for the mutants were located. All of the strains were then tested for pathogenicity by the following assays: calcium dependence, colonization of the mouse gastrointestinal tract, HEp-2 cell adherence and invasion, HEp-2 cell monolayer detachment, autoagglutination, serum resistance, outer membrane protein production and production of V antigen. In addition, the hydrophobic properties of each strain were examined by a rapid polystyrene plate method and hydrophobic interaction chromatography. The results of the tests were compared to plasmid DNA analyses for each strain in an attempt to identify any plasmid-associated genes that are related to virulence. The wild-type strain was virulent, or positive, by all of the assays employed for evaluation of pathogenicity. In contrast, its isogenic derivative that had been cured of the virulence plasmid was negative, or avirulent, for the same assays with one exception. The avirulent plasmidless strain still retained the ability to adhere to and invade HEp-2 cells, supporting the belief that these properties are probably encoded by the bacterial chromosome. In addition, three transpositional mutants were constructed that were no longer calcium dependent, capable of detaching HEp-2 cell monolayers or able to produce three unique outer membrane proteins. Restriction endonuclease analysis confirmed the presence of the transposon on the Hind III "A" fragment of the virulence plasmid and located the region responsible for the lost virulence properties. The gene or set of genes identified were designated cal and the respective calcium independent mutants Cal⁻. Furthermore, the assays for hydrophobicity indicated that the virulence plasmid, specifically the cal gene(s), codes for hydrophobic properties on the surface of the bacterium. The study demonstrated that a virulence-associated region, cal, is located on the virulence plasmid of Y. entrocolitica and responsible for calcium dependence, HEp-2 cell monolayer detachment, the production of the three plasmid-specified outer membrane proteins and cell-surface hydrophobicity.
Subjects/Keywords: Plasmids.;
Plasmids – Genetics.;
Viruses.;
Viral genetics.;
Yersinia enterocolitica – Physiology.
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APA ·
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APA (6th Edition):
DUBEL, J. R. (1983). MOLECULAR AND GENETIC CHARACTERIZATION OF THE VIRULENCE PLASMID OF YERSINIA ENTEROCOLITICA.
(Doctoral Dissertation). University of Arizona. Retrieved from http://hdl.handle.net/10150/187348
Chicago Manual of Style (16th Edition):
DUBEL, JACQUELINE ROBERTA. “MOLECULAR AND GENETIC CHARACTERIZATION OF THE VIRULENCE PLASMID OF YERSINIA ENTEROCOLITICA.
” 1983. Doctoral Dissertation, University of Arizona. Accessed February 27, 2021.
http://hdl.handle.net/10150/187348.
MLA Handbook (7th Edition):
DUBEL, JACQUELINE ROBERTA. “MOLECULAR AND GENETIC CHARACTERIZATION OF THE VIRULENCE PLASMID OF YERSINIA ENTEROCOLITICA.
” 1983. Web. 27 Feb 2021.
Vancouver:
DUBEL JR. MOLECULAR AND GENETIC CHARACTERIZATION OF THE VIRULENCE PLASMID OF YERSINIA ENTEROCOLITICA.
[Internet] [Doctoral dissertation]. University of Arizona; 1983. [cited 2021 Feb 27].
Available from: http://hdl.handle.net/10150/187348.
Council of Science Editors:
DUBEL JR. MOLECULAR AND GENETIC CHARACTERIZATION OF THE VIRULENCE PLASMID OF YERSINIA ENTEROCOLITICA.
[Doctoral Dissertation]. University of Arizona; 1983. Available from: http://hdl.handle.net/10150/187348
University of North Texas
30.
Poulter, Melinda D.
Structural Analysis of the TOL pDK1 xylGFJQK Region and Partial Characterization of the xylF and xylG Gene Products.
Degree: 1999, University of North Texas
URL: https://digital.library.unt.edu/ark:/67531/metadc2270/
► TOL plasmids encode enzymes responsible for utilization of toluene and related aromatic compounds by Pseudomonas putida, ultimately converting them to central metabolic intermediates. The nucleotide…
(more)
▼ TOL
plasmids encode enzymes responsible for utilization of toluene and related aromatic compounds by Pseudomonas putida, ultimately converting them to central metabolic intermediates. The nucleotide sequence for the 5.6 kb xylGFJQK region of the pDK1 TOL meta operon was determined. DNA sequence analysis revealed the presence of five open reading frames corresponding to xylG (1458 bp), xylF (846 bp), xylJ (783 bp), xylQ (936 bp) and xylK (1047 bp), encoding predicted protein products of 51.6, 31.3, 27.8, 32.8, and 36.6 kDa in size, respectively. The average G+C content of the xylLTEGFJQK region was 65.7%, somewhat higher than the 58.9% seen in the immediately upstream xylXYZ region and substantially more than the 50% G+C content reported for the upper TOL operon of this plasmid. Homology comparisons were made with genes and proteins of related catabolic
plasmids. The dmpCDEFG and pWWO xylGFJQK regions exhibit consistently high levels of nucleotide and amino acid homology to pDK1 xylGFJQK throughout the entire region. In contrast, although the nucleotide sequence homology of the Acinetobacter atdCDE region to xylGFJ is high, the homology of atdFG to xylQK is markedly less. Such radical changes in homology between corresponding regions of different operons, combined with variable base and codon usage patterns within and between operons, provides additional support for the idea that the upper and lower operons encoding enzymes of aromatic pathways have evolved independently of one another and that these operons have continued to exchange genetic material with homologous expression units through a series of recombination events. Recombinant
plasmids were constructed for individual expression of each of the xylGFJQK genes. HMSD (XylG) and HMSH (XylF) were partially purified and characterized with respect to substrate specificity and kinetic mechanism. Evidence was obtained suggesting that the HMSD reaction occurs via a steady state ordered mechanism or a random mechanism where binding of the first substrate effects the enzyme's affinity for the second substrate.
Advisors/Committee Members: Benjamin, Robert C., Knesek, John, O'Donovan, Gerard A., Pirtle, Robert M., Zimmerman, Earl G..
Subjects/Keywords: Plasmids – Genetics.; Pseudomonas putida.; recombinant plasmids; DNA research
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Open Access Theses and Dissertations
