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University of Guelph
1.
Ficht, Alexandra.
Genetic Analysis of Sucrose Concentration in Soybean Seeds Using a Historical Soybean Genomic Panel
.
Degree: 2018, University of Guelph
URL: https://atrium.lib.uoguelph.ca/xmlui/handle/10214/13023 
► Sucrose concentration in soybean (Glycine max L. Merr) seed is becoming an increasingly important trait for the soy food industry. The objectives of this thesis…
(more)
▼ Sucrose concentration in soybean (Glycine max L. Merr) seed is becoming an increasingly important trait for the soy food industry. The objectives of this thesis were to: 1) identify quantitative trait loci (QTL) for sucrose concentration, 2) explore the allelic variation present in a genomic panel for candidate genes related to sucrose synthesis in soybean, and 3) determine the effect of genotype (G), environment (E) and genotype-by-environment interaction (GE) on varying sucrose levels in the seed. A University of Guelph panel of 296 pedigree-related genotypes was used for this analysis. Significant G, E, and GE interaction effects were observed for sucrose over three years and four locations in Ontario, which allowed for determining the trait stability. QTL analysis was performed using single nucleotide polymorphisms (SNPs) detected using a genotyping-by-sequencing (GBS) approach. A putative QTL was identified in the genomic region harbouring a potential gene involved in sucrose metabolism. Improved understanding of the
genetics of sucrose biosynthesis allows for the future development of high sucrose soybean cultivars.
Advisors/Committee Members: Rajcan, Istvan (advisor).
Subjects/Keywords: Plant Breeding;
Genetics
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APA (6th Edition):
Ficht, A. (2018). Genetic Analysis of Sucrose Concentration in Soybean Seeds Using a Historical Soybean Genomic Panel
. (Thesis). University of Guelph. Retrieved from https://atrium.lib.uoguelph.ca/xmlui/handle/10214/13023
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Ficht, Alexandra. “Genetic Analysis of Sucrose Concentration in Soybean Seeds Using a Historical Soybean Genomic Panel
.” 2018. Thesis, University of Guelph. Accessed September 17, 2019.
https://atrium.lib.uoguelph.ca/xmlui/handle/10214/13023.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Ficht, Alexandra. “Genetic Analysis of Sucrose Concentration in Soybean Seeds Using a Historical Soybean Genomic Panel
.” 2018. Web. 17 Sep 2019.
Vancouver:
Ficht A. Genetic Analysis of Sucrose Concentration in Soybean Seeds Using a Historical Soybean Genomic Panel
. [Internet] [Thesis]. University of Guelph; 2018. [cited 2019 Sep 17].
Available from: https://atrium.lib.uoguelph.ca/xmlui/handle/10214/13023.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Ficht A. Genetic Analysis of Sucrose Concentration in Soybean Seeds Using a Historical Soybean Genomic Panel
. [Thesis]. University of Guelph; 2018. Available from: https://atrium.lib.uoguelph.ca/xmlui/handle/10214/13023
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
2.
Sood, Ritu.
Genetic analysis of morphological biochemical and
molecular traits of some novelty rices of Himachal
Pradesh;.
Degree: 2004, INFLIBNET
URL: http://shodhganga.inflibnet.ac.in/handle/10603/10183
► newline ABSTRACT newline newline Genetic analysis of morphological, biochemical and molecular traits of some novelty rices of Himachal Pradesh was carried out by using 17…
(more)
▼ newline ABSTRACT newline newline Genetic analysis
of morphological, biochemical and molecular traits of some novelty
rices of Himachal Pradesh was carried out by using 17 red pericarp
rices, 19 purple leaved rices and 11 quality rices along with three
checks in Randomized Block Design at Rice and Wheat Research
Centre, Malan (Kangra). The data was analyzed using statistical
procedures. Based on overall performance, Achhoo Baldhar,
IC3131159, Kijun and Tiyun among red pericarp rices; RLC-3, Lal
Nakanda-41, R-575 and Purple Baldhar among purple leaved rices and
LC99-5B, IC3131155 and Rajpur Basmati among quality rices were
found to be promising genotypes for further exploitation. Tiyun had
high sugar (10.4 mg/100g) and high protein content (14.0%). R-575
had high 1000-grain weight (32.1 g). IC3131166 and Rajpur Basmati
had long slender grains, high elongation after cooking and high
brown rice protein. Grain yield had significant positive
correlation with grains per panicle, plant height and spikelets per
panicle thus direct selection for those characters will be
effective. Based on path coefficient analysis tillers per plant,
panicle length, grains per panicle and 1000-grain weight had high
positive direct effects on yield. Molecular analysis based on RAPD
markers grouped 41 genotypes into cluster I, 25 of these were
grouped into cluster II of ISSR and 33 in cluster I of
D2-statistics (combined over years). This suggested similarity in
clustering pattern based on these techniques. Grouping of majority
of the genotypes into one cluster suggested affinity among the
genotypes indicating their origin in same geographical area.
Grouping based on pooling two types of markers revealed that
majority of the red pericarp rices and purple leaved rices grouped
into two distinct sub-clusters. Tremendous variation in anthocyanin
pigmentation of different plant parts was present in all red,
purple and quality rices. Among check variation was present only in
case of size of auricle. Auricles were absent in Achhoo and Achhoo
Baldhar. Varieties namely, China Purple, HPR-1194, HPR-2089,
HPR-2178, Palampur Purple and Nagrota Purple had dark purple leaf
blade, purple auricle, purple stigma and purple apiculus. Achhoo
Baldhar had long grains with dark red pericarp, whereas IC3131171
and IC3131159 had light red pericarp and long grains. newline
newline newline¬¬¬¬¬¬¬_______________________ _____________________
newlineSignature of student with date Signature of Major Advisor
newline newlineCountersigned newline newline________________
newlineHead of Department newline newline
Advisors/Committee Members: kaushik, R.P..
Subjects/Keywords: Plant breeding; Plant genetics; Rice
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APA (6th Edition):
Sood, R. (2004). Genetic analysis of morphological biochemical and
molecular traits of some novelty rices of Himachal
Pradesh;. (Thesis). INFLIBNET. Retrieved from http://shodhganga.inflibnet.ac.in/handle/10603/10183
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Sood, Ritu. “Genetic analysis of morphological biochemical and
molecular traits of some novelty rices of Himachal
Pradesh;.” 2004. Thesis, INFLIBNET. Accessed September 17, 2019.
http://shodhganga.inflibnet.ac.in/handle/10603/10183.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Sood, Ritu. “Genetic analysis of morphological biochemical and
molecular traits of some novelty rices of Himachal
Pradesh;.” 2004. Web. 17 Sep 2019.
Vancouver:
Sood R. Genetic analysis of morphological biochemical and
molecular traits of some novelty rices of Himachal
Pradesh;. [Internet] [Thesis]. INFLIBNET; 2004. [cited 2019 Sep 17].
Available from: http://shodhganga.inflibnet.ac.in/handle/10603/10183.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Sood R. Genetic analysis of morphological biochemical and
molecular traits of some novelty rices of Himachal
Pradesh;. [Thesis]. INFLIBNET; 2004. Available from: http://shodhganga.inflibnet.ac.in/handle/10603/10183
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
3.
Damodran, Suresh.
Regulation of GmHD-ZIPIII During Soybean Root Lateral Organ Development.
Degree: MS, Plant Science, 2012, South Dakota State University
URL: http://openprairie.sdstate.edu/etd/1339
► Biological nitrogen fixation in symbiotic nodules of leguminous crops viz. soybean reduces the use of chemical fertilizers providing economic and environmental benefits. Though not…
(more)
▼ Biological nitrogen fixation in symbiotic nodules of leguminous crops viz. soybean reduces the use of chemical fertilizers providing economic and environmental benefits. Though not completely understood, the molecular signaling events during nodule development are being identified. Class III homeodomain leucine zipper (HDZIPIII) proteins are a group of
plant specific transcriptional factors that are known to regulate several developmental process in Arabidopsis and other plants. Transcriptional, post-transcriptional and post-translational regulation of two GmHD-ZIPIII genes (GmHD-ZIPIII-1 & GmHD-ZIPIII-2) was examined in soybean roots inoculated with its symbiont Bradyrhizobium japonicum, to understand their role in root and nodule development. The expression of these genes was monitored using promoter:GUS and promoter:tdTomato constructs in hairy root composite soybean plants, at different time points post B. japonicum inoculation. These genes were expressed in the root tips, root vasculature, emerging lateral root, emerging nodule and vasculature of mature nodules suggesting a possible role of GmHD-ZIPIII in root lateral organ development. Examination of GmHD-ZIPIII gene expression along a time course of nodule development suggested that GmHD-ZIPIII-2 is up-regulated by B. japonicum inoculation. HD-ZIPIIIs are regulated in a post-transcriptional manner by miR166 in many
plant species. Cleavage of GmHD-ZIPIII transcripts by miR166 was experimentally validated using 5'-RLM-RACE assays. The expression levels of miR166 and the target GmHD-ZIPIIIs were identified using qRT-PCR and RNA sequencing respectively in emerging lateral roots, young lateral roots, emerging nodules and mature nodules. The results suggested that GmHD-ZIPIIIs are expressed at higher levels in emerging lateral organs compared to the respective mature organs. Examination of miR166 activity using a miR166 sensor suggested that miR166 spatially restricts GmHDZIPIII expression to the metaxylem and epidermis in the roots as observed in Arabidopsis. In symbiotic nodules, miR166 appears to spatially restrict GmHD-ZIPIII expression to the basal part of the nodule vasculature. The activity of HD-ZIPIII proteins are regulated in a post translational manner by small leucine zipper proteins (ZPRs). The interaction of GmHD-ZIPIII-1 & 2 proteins with all eight predicted ZPR proteins in soybean was examined using yeast-two-hybrid assay. Both GmHD-ZIPIII genes showed positive interaction with ZPR3b, ZPR3c and ZPR3d. Quantitative assays of interaction based on β-GAL activity (a marker gene in the assay) indicated that ZPR3d and GmHDZIPIII- 2 pair had the strongest interaction among the tested GmHD-ZIPIII and ZPR pairs. These observations suggested that GmHD-ZIPIII genes are indeed regulated at the transcriptional, post-transcriptional and post-translational levels in soybean. Subsequent experiments to examine significance of GmHD-ZIPIII regulation during nodule development would provide crucial insights in this crucial process.
Advisors/Committee Members: Senthil Subramanian.
Subjects/Keywords: Plant Breeding and Genetics
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APA (6th Edition):
Damodran, S. (2012). Regulation of GmHD-ZIPIII During Soybean Root Lateral Organ Development. (Masters Thesis). South Dakota State University. Retrieved from http://openprairie.sdstate.edu/etd/1339
Chicago Manual of Style (16th Edition):
Damodran, Suresh. “Regulation of GmHD-ZIPIII During Soybean Root Lateral Organ Development.” 2012. Masters Thesis, South Dakota State University. Accessed September 17, 2019.
http://openprairie.sdstate.edu/etd/1339.
MLA Handbook (7th Edition):
Damodran, Suresh. “Regulation of GmHD-ZIPIII During Soybean Root Lateral Organ Development.” 2012. Web. 17 Sep 2019.
Vancouver:
Damodran S. Regulation of GmHD-ZIPIII During Soybean Root Lateral Organ Development. [Internet] [Masters thesis]. South Dakota State University; 2012. [cited 2019 Sep 17].
Available from: http://openprairie.sdstate.edu/etd/1339.
Council of Science Editors:
Damodran S. Regulation of GmHD-ZIPIII During Soybean Root Lateral Organ Development. [Masters Thesis]. South Dakota State University; 2012. Available from: http://openprairie.sdstate.edu/etd/1339
4.
Wilson, Nicholas Charles.
The distribution, growth, reproduction and population genetics of a mangrove species, Rhizophora stylosa Griff, near its southern limits in New South Wales, Australia.
Degree: PhD, 2009, Australian Catholic University
URL: https://researchbank.acu.edu.au/theses/268
► Rhizophora stylosa is a common and widespread mangrove species on tropical coasts in the Indo-West Pacific and very common and widespread in northern Australia. This…
(more)
▼ Rhizophora stylosa is a common and widespread mangrove species on tropical coasts in the Indo-West Pacific and very common and widespread in northern Australia. This aim of this study was to investigate R. stylosa's distribution, growth, reproduction and population genetics over the last 300 kms of its range at its southern limits in northern New South Wales on the Australian east coast. Rhizophora stylosa was found to be more widespread between and within estuaries in northern New South Wales than previously recorded, with evident spread over recent decades. A new southern limit of South West Rocks Creek was determined, with only two mature trees are present. Rhizophora stylosa is in small numbers in most the 16 estuaries now known to contain R. stylosa in NSW. Its distribution is illustrated from intra-estuarine scales in small 'incipient' stands developing within estuaries to the colonisation of new estuaries. Despite the evidence of recent population spread, several restricted old stands were recorded, indicating a limited presence of R. stylosa at high latitudes for much longer periods, somewhat complicating a simple model of expanding range with recently warming climate. Mangrove species are generally understood to be limited by cold, but significant provisos on ascribing R. stylosa's recent spread to the warming trends of the last several decades exist. A shoot tracking methodology was applied for 2.5 years in three estuaries, including the most southerly, and detected little if any reduction of growth over a range of 260 km. Rhizophora stylosa has a reiterative mode of growth and an average of three leaf emergences (6 leaves) per annum was found. The growth results overall are comparable to some tropical studies, particularly if herbivory is accounted for, and R. stylosa appears not to be at its thermal tolerance at its known southern limits.;Leaf longevity was greater than comparable tropical studies, suggesting the trees were compensating for climatic factors in this manner. A reproductive study conducted at the same time on the same shoots found trees reproduce successfully even at the southern limits, with little or no evidence of a decline with latitude across the study area. Only a small proportion of buds finally form propagules (overall 2.2%), but again fecundity is comparable to tropical studies, although the full reproductive cycle from flower bud primordia to viviparous propagule may be slightly longer. A generally low level of genetic diversity measured as allelic richness and heterozygosity and evidence of inbreeding was detected during the genetic study on samples from eight New South Wales estuaries, three localities in Moreton Bay and eight localities in north Queensland. Spatially, the New South Wales and Moreton Bay localities are from one pool, but there is differentiation between localities and little geographic coherence along the coast. Different origins and histories are likely for the estuaries, supporting a hypothesis arising from the distribution of R. stylosa in New South Wales that…
Subjects/Keywords: Plant Breeding and Genetics
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APA ·
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APA (6th Edition):
Wilson, N. C. (2009). The distribution, growth, reproduction and population genetics of a mangrove species, Rhizophora stylosa Griff, near its southern limits in New South Wales, Australia. (Doctoral Dissertation). Australian Catholic University. Retrieved from https://researchbank.acu.edu.au/theses/268
Chicago Manual of Style (16th Edition):
Wilson, Nicholas Charles. “The distribution, growth, reproduction and population genetics of a mangrove species, Rhizophora stylosa Griff, near its southern limits in New South Wales, Australia.” 2009. Doctoral Dissertation, Australian Catholic University. Accessed September 17, 2019.
https://researchbank.acu.edu.au/theses/268.
MLA Handbook (7th Edition):
Wilson, Nicholas Charles. “The distribution, growth, reproduction and population genetics of a mangrove species, Rhizophora stylosa Griff, near its southern limits in New South Wales, Australia.” 2009. Web. 17 Sep 2019.
Vancouver:
Wilson NC. The distribution, growth, reproduction and population genetics of a mangrove species, Rhizophora stylosa Griff, near its southern limits in New South Wales, Australia. [Internet] [Doctoral dissertation]. Australian Catholic University; 2009. [cited 2019 Sep 17].
Available from: https://researchbank.acu.edu.au/theses/268.
Council of Science Editors:
Wilson NC. The distribution, growth, reproduction and population genetics of a mangrove species, Rhizophora stylosa Griff, near its southern limits in New South Wales, Australia. [Doctoral Dissertation]. Australian Catholic University; 2009. Available from: https://researchbank.acu.edu.au/theses/268
Wilfrid Laurier University
5.
Long, Chengli.
The Pleiotropic Mutant R50 (sym16): A Link Between Cytokinin and Starch Metabolism May Explain Its Seed Phenotype.
Degree: 2010, Wilfrid Laurier University
URL: https://scholars.wlu.ca/etd/1027
► Cytokinins are a class of adenine-based plant hormones that stimulate cell divisions and are therefore essential for organ growth and development. The role that cytokinin…
(more)
▼ Cytokinins are a class of adenine-based plant hormones that stimulate cell divisions and are therefore essential for organ growth and development. The role that cytokinin plays in nodulation and that of R50 (syml6) in particular is very complex. In this study, a cytokinin antagonist and a cytokinin oxidase (CKX) inhibitor were used as tools to study the effects of cytokinin perception or cytokinin degradation on nodulation in pea, the cytokinin transduction pathway, especially that induced by CRE1/AHK4, and CKX play a significant role in pea nodulation. Comparison of the full length sequences PsCKX2 cDNAs from Sparkle and R50 indicated that PsCKX2 is likely not syml6. The availability of the full length PsCKX2 cDNA facilitated the assessment of PsCKX1 and PsCKX2 expression profiles in seedling development using semi-quantitative RT-PCR. Along with a morphological study (dry weight of R50 cotyledons are not decreasing as fast as those of wild type) and microscopic studies (R50 starch grains within the cotyledons are not digested as fast as those of wild type) of seedling development, a workmg model which integrates cytokinm and starch metabolism is proposed to explain the seedlmg phenotype of R50.
Subjects/Keywords: Plant Breeding and Genetics
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APA (6th Edition):
Long, C. (2010). The Pleiotropic Mutant R50 (sym16): A Link Between Cytokinin and Starch Metabolism May Explain Its Seed Phenotype. (Thesis). Wilfrid Laurier University. Retrieved from https://scholars.wlu.ca/etd/1027
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Long, Chengli. “The Pleiotropic Mutant R50 (sym16): A Link Between Cytokinin and Starch Metabolism May Explain Its Seed Phenotype.” 2010. Thesis, Wilfrid Laurier University. Accessed September 17, 2019.
https://scholars.wlu.ca/etd/1027.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Long, Chengli. “The Pleiotropic Mutant R50 (sym16): A Link Between Cytokinin and Starch Metabolism May Explain Its Seed Phenotype.” 2010. Web. 17 Sep 2019.
Vancouver:
Long C. The Pleiotropic Mutant R50 (sym16): A Link Between Cytokinin and Starch Metabolism May Explain Its Seed Phenotype. [Internet] [Thesis]. Wilfrid Laurier University; 2010. [cited 2019 Sep 17].
Available from: https://scholars.wlu.ca/etd/1027.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Long C. The Pleiotropic Mutant R50 (sym16): A Link Between Cytokinin and Starch Metabolism May Explain Its Seed Phenotype. [Thesis]. Wilfrid Laurier University; 2010. Available from: https://scholars.wlu.ca/etd/1027
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Wilfrid Laurier University
6.
Wright, Michael.
The Evolution of Sexual Reproduction in Cuscuta (Convolvulaceae).
Degree: 2011, Wilfrid Laurier University
URL: https://scholars.wlu.ca/etd/1039
► Cuscuta (Convolvulaceae), the dodders, is a genus of ca. 200 species of obligate stem parasites distributed across a great diversity of habitats worldwide. The existence…
(more)
▼ Cuscuta (Convolvulaceae), the dodders, is a genus of ca. 200 species of obligate stem parasites distributed across a great diversity of habitats worldwide. The existence of a handful of species that are dangerous crop weeds has led researchers to historically focus on their growth and control. Consequently, there is a dearth of information about their biodiversity, ecology, and in particular their reproductive biology. This thesis surveys aspects of sex allocation, floral evolution, floral rewards for pollinators, and mechanisms of reproductive assurance across the genus. I demonstrate that Cuscuta has evolved a broad spectrum of breeding systems, from obligate selfing to obligate outcrossing. Predictions made by sex allocation theory of negative correlations between pollen number and pollen grain size, and between male investment and female investment are shown to be false in Cuscuta. Histological examination of the floral nectary demonstrates that it is typical in structure, and I predict that it is functional in most facultatively and obligately xenogamous species. Cuscuta pollen is variable in the proportions of lipid and starch reserves, and has a sticky external pollenkitt. The role of the infrastaminal scales is narrowed to 1) defense against seed predators, and 2) a shield against early self-pollination in some strongly protandrous species. Lastlly, I demonstrate that the evolution of two styles, followed by unequal styles in Cuscuta, were critical for the radiation of the genus. The more flexible floral design enabled Cuscuta to evolve different mechanisms of reproductive assurance in coordination with their exploitation of novel host species and new pollination environments.
Subjects/Keywords: Plant Breeding and Genetics
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APA (6th Edition):
Wright, M. (2011). The Evolution of Sexual Reproduction in Cuscuta (Convolvulaceae). (Thesis). Wilfrid Laurier University. Retrieved from https://scholars.wlu.ca/etd/1039
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Wright, Michael. “The Evolution of Sexual Reproduction in Cuscuta (Convolvulaceae).” 2011. Thesis, Wilfrid Laurier University. Accessed September 17, 2019.
https://scholars.wlu.ca/etd/1039.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Wright, Michael. “The Evolution of Sexual Reproduction in Cuscuta (Convolvulaceae).” 2011. Web. 17 Sep 2019.
Vancouver:
Wright M. The Evolution of Sexual Reproduction in Cuscuta (Convolvulaceae). [Internet] [Thesis]. Wilfrid Laurier University; 2011. [cited 2019 Sep 17].
Available from: https://scholars.wlu.ca/etd/1039.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Wright M. The Evolution of Sexual Reproduction in Cuscuta (Convolvulaceae). [Thesis]. Wilfrid Laurier University; 2011. Available from: https://scholars.wlu.ca/etd/1039
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
University of Minnesota
7.
Ziyomo, Cathrine.
Genetic analysis to improve drought and low nitrogen tolerance of corn in monoculture and in a kura clover intercropping system.
Degree: PhD, Applied plant sciences, 2012, University of Minnesota
URL: http://purl.umn.edu/144409
► Direct selection for grain yield under stress conditions is often inefficient because the heritability for grain yield is greatly reduced under stress. The objectives of…
(more)
▼ Direct selection for grain yield under stress conditions is often inefficient because the heritability for grain yield is greatly reduced under stress. The objectives of the first study described in this thesis were to determine the efficiency of indirect selection for corn (Zea mays L.) grain yield under drought and low N conditions using secondary traits or molecular markers. Testcrosses of 238 intermated B73 x Mo17 recombinant inbreds were evaluated under drought and low N stress conditions. Results indicated that direct selection for grain yield in the targeted stress environment is more efficient than using secondary traits under both drought stress and low N stress. Using significant markers only was not more efficient than direct phenotypic selection for grain yield. The relative efficiency of genomewide selection was significantly greater than 1.0 for grain yield under drought stress but not for grain yield under low N stress. The results suggest that selection based on molecular markers is more efficient than phenotypic selection alone for the improvement of grain yield under drought stress, while for grain yield under low N stress, selection based on markers alone can only be more efficient if gains per unit time and cost are considered. In addition to the genetic improvement of corn for stress tolerance, the use of drought tolerant corn in a kura clover (Trifolium ambiguum M. Bieb.) intercropping system can reduce the competition for moisture between the grain crop and the cover crop. The objectives of the second study were to determine if drought tolerant corn can minimize the yield losses incurred when corn is intercropped with kura clover. Results indicate that drought-tolerant corn can maintain high yields and allow sufficient regrowth of kura clover and therefore significantly reduce the risk associated with intercropping corn with living mulch.
Subjects/Keywords: Plant breeding; Quantitative genetics
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APA (6th Edition):
Ziyomo, C. (2012). Genetic analysis to improve drought and low nitrogen tolerance of corn in monoculture and in a kura clover intercropping system. (Doctoral Dissertation). University of Minnesota. Retrieved from http://purl.umn.edu/144409
Chicago Manual of Style (16th Edition):
Ziyomo, Cathrine. “Genetic analysis to improve drought and low nitrogen tolerance of corn in monoculture and in a kura clover intercropping system.” 2012. Doctoral Dissertation, University of Minnesota. Accessed September 17, 2019.
http://purl.umn.edu/144409.
MLA Handbook (7th Edition):
Ziyomo, Cathrine. “Genetic analysis to improve drought and low nitrogen tolerance of corn in monoculture and in a kura clover intercropping system.” 2012. Web. 17 Sep 2019.
Vancouver:
Ziyomo C. Genetic analysis to improve drought and low nitrogen tolerance of corn in monoculture and in a kura clover intercropping system. [Internet] [Doctoral dissertation]. University of Minnesota; 2012. [cited 2019 Sep 17].
Available from: http://purl.umn.edu/144409.
Council of Science Editors:
Ziyomo C. Genetic analysis to improve drought and low nitrogen tolerance of corn in monoculture and in a kura clover intercropping system. [Doctoral Dissertation]. University of Minnesota; 2012. Available from: http://purl.umn.edu/144409
Eastern Illinois University
8.
Parks, Tyler.
An Efficient Protocol for Root Studies in the Common Sunflower Using Composite Plants.
Degree: MS, Biological Sciences, 2018, Eastern Illinois University
URL: https://thekeep.eiu.edu/theses/4405
► Sunflower, Helianthus annuus, is one of the most important oilseed crop in the world. Oil from the seeds is prized for its' exceptional quality…
(more)
▼ Sunflower,
Helianthus annuus, is one of the most important oilseed crop in the world. Oil from the seeds is prized for its' exceptional quality and flavor. Despite this, sunflowers have been forced onto marginal lands often on semi-arid and non-fertile soil, due to disease pressures and economics making it critical to produce more resilient lines and varieties to withstand these new stresses. The recent availability of the sunflower genome can allow genome-wide characterization of gene families. Stable transformation protocols, which can be used for characterization studies, have been developed for
H. annuus using
Agrobacterium tumefaciens, but they produce low efficiency and are time-consuming. For this reason, very few functional studies have been done in the sunflower. Composite plants, produced using
Agrobacterium rhizogenes, are plants with transgenic 'hairy' roots and wild type shoots. Composite plants offer benefits over creating fully transgenic plants, namely time and cost. With
plant transformation usually being the rate limiting step for gene functional studies of non-model plants, composite plants can alleviate this bottleneck. Originally produced
in vitro, the method developed here outlines a less expensive
ex vitro alternative to produce composite sunflowers for study. The critical steps and parameters are outlined and discussed further herein. The testing of more than a dozen genotypes validates the utility and efficiency of this protocol. We also study the performance of three constitutive promoters for the expression of genes of interest. The functional characterization of a root specific transcription factor HaLBD16, via overexpression and RNAi silencing, further reinforce the utility of the system.
Advisors/Committee Members: Yordan S. Yordanov, Barbara S. Carlsward, Thomas Canam.
Subjects/Keywords: Plant Breeding and Genetics
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APA (6th Edition):
Parks, T. (2018). An Efficient Protocol for Root Studies in the Common Sunflower Using Composite Plants. (Masters Thesis). Eastern Illinois University. Retrieved from https://thekeep.eiu.edu/theses/4405
Chicago Manual of Style (16th Edition):
Parks, Tyler. “An Efficient Protocol for Root Studies in the Common Sunflower Using Composite Plants.” 2018. Masters Thesis, Eastern Illinois University. Accessed September 17, 2019.
https://thekeep.eiu.edu/theses/4405.
MLA Handbook (7th Edition):
Parks, Tyler. “An Efficient Protocol for Root Studies in the Common Sunflower Using Composite Plants.” 2018. Web. 17 Sep 2019.
Vancouver:
Parks T. An Efficient Protocol for Root Studies in the Common Sunflower Using Composite Plants. [Internet] [Masters thesis]. Eastern Illinois University; 2018. [cited 2019 Sep 17].
Available from: https://thekeep.eiu.edu/theses/4405.
Council of Science Editors:
Parks T. An Efficient Protocol for Root Studies in the Common Sunflower Using Composite Plants. [Masters Thesis]. Eastern Illinois University; 2018. Available from: https://thekeep.eiu.edu/theses/4405
University of Manitoba
9.
Cuthbert, Richard.
Molecular mapping of septoria tritici blotch resistance in hexaploid wheat (Triticum aestivum L.).
Degree: Plant Science, 2011, University of Manitoba
URL: http://hdl.handle.net/1993/4760
► Septoria tritici blotch (Stb) is a major foliar disease of wheat worldwide caused by the fungal pathogen Mycosphaerella graminicola. This project mapped the chromosomal locations…
(more)
▼ Septoria tritici blotch (Stb) is a major foliar disease of wheat worldwide caused by the fungal pathogen Mycosphaerella graminicola. This project mapped the chromosomal locations of Stb resistance, which will be useful for wheat cultivar enhancement. In the first study of this project, Stb resistance and grain yield quantitative trait loci (QTL) were identified in a winter wheat doubled-haploid (DH) population produced from the cultivars Pastiche (resistant) and Torfrida (susceptible). A genetic map of the population was constructed using 104 microsatellite and 202 DArT markers. Separate disease and yield field trials were conducted in north-western Europe to measure natural Stb infection and grain yield, respectively. A resistance QTL from Pastiche was identified (QStb.jic-2A) that reduced flag leaf pycnidial density by 31.2% compared to the population mean. A second QTL from Pastiche (QYld.jic-2A) co-localized with QStb.jic-2A, and conferred an increase in grain yield of approximately 2.0% (0.19 t ha-1).
The second study determined the map location of the third multiple-isolate resistance gene in the hexaploid landrace Salamouni. A population was developed using the resistant DH line 98S05B*13 (Salamouni/Katepwa) crossed to the susceptible cultivar Katepwa. The parent 98S05B*13 was found to contain the resistance gene Stb14 on chromosome 3BS, therefore, to minimize the effect of this gene, individual plants of the F2 population were selected for self-pollination based on presence or absence of Stb14 using flanking microsatellite markers. The Stb14(+) F2:3 families were screened with M. graminicola isolate MG96-36. Bulked segregant analysis identified a possible linkage to the gene of interest on chromosome 3AS. Polymorphic microsatellite markers on 3AS were used to construct a linkage group. The markers barc321 and barc12 were found to flank the resistance gene at genetic distances of 1.9 and 2.5 cM, respectively. This position on 3AS has not been previously linked to Stb resistance and this gene will be designated StbSm3.
The third population was developed to fine map the isolate-specific resistance gene Stb14 located on chromosome 3B of Salamouni. A large population of 84 fixed recombinant F4 families was developed using the co-dominant microsatellite markers flanking Stb14. Attempts to identify clearly polymorphic molecular markers within the interval containing Stb14 were unsuccessful.
Advisors/Committee Members: Brûlé-Babel, Anita (Plant Science) (supervisor), Somers, Daryl (Plant Science) Gilbert, Jeannie (Biological Sciences) Goodwin, Stephen (Purdue University) (examiningcommittee).
Subjects/Keywords: Genetics; Pathology; Plant Breeding
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APA (6th Edition):
Cuthbert, R. (2011). Molecular mapping of septoria tritici blotch resistance in hexaploid wheat (Triticum aestivum L.). (Thesis). University of Manitoba. Retrieved from http://hdl.handle.net/1993/4760
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Cuthbert, Richard. “Molecular mapping of septoria tritici blotch resistance in hexaploid wheat (Triticum aestivum L.).” 2011. Thesis, University of Manitoba. Accessed September 17, 2019.
http://hdl.handle.net/1993/4760.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Cuthbert, Richard. “Molecular mapping of septoria tritici blotch resistance in hexaploid wheat (Triticum aestivum L.).” 2011. Web. 17 Sep 2019.
Vancouver:
Cuthbert R. Molecular mapping of septoria tritici blotch resistance in hexaploid wheat (Triticum aestivum L.). [Internet] [Thesis]. University of Manitoba; 2011. [cited 2019 Sep 17].
Available from: http://hdl.handle.net/1993/4760.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Cuthbert R. Molecular mapping of septoria tritici blotch resistance in hexaploid wheat (Triticum aestivum L.). [Thesis]. University of Manitoba; 2011. Available from: http://hdl.handle.net/1993/4760
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
University of KwaZulu-Natal
10.
[No author].
Genetic diversity of proprietary inbred lines of sunflower, determined by mapped SSR markers and total protein analysis.
Degree: Plant breeding, 2008, University of KwaZulu-Natal
URL: http://hdl.handle.net/10413/733
► This study compared DNA based SSR markers with total seed protein markers, used to evaluate genetic diversity of sunflower. The multiplex-ability, cost effectiveness and applicability…
(more)
▼ This study compared DNA based SSR markers with total seed protein markers, used to evaluate genetic diversity of sunflower. The multiplex-ability, cost effectiveness and applicability of microsatellites as molecular markers for a genetic diversity study were investigated and evaluated based on pedigree data of the sunflower germplasm. A solution for oil and fat interference in ultrathin iso-electric focusing gels was investigated, in order to make imaging and interpretation easier and clearer. Total protein analysis was utilized for the determination of genetic diversity on the same inbred material used for the DNA analysis. Finally a correlation is made between the data obtained on DNA vs Protein compared with phenotype and expected pedigree data. A set of 73 SSR markers with known mapped positions were utilized to determine genetic similarity in a group of sunflower inbred lines. Cluster analysis of genetic similarity revealed an excellent correlation with the
breeding background and source information obtained from breeders on all inbred lines used in this study. Cluster analysis gave a clear differentiation between B and R-lines, showing clearly defined heterotic groups of the proprietary set of inbred lines. The most outstanding single-locus SSR markers in the set used for this study were identified and used as a core set. Multiplex assays were designed and optimized for the most cost and time effective method for rapid variety identification. The selected markers produced robust PCR products, amplified a single locus each, were polymorphic among the elite inbred lines and supplied a good, genome-wide framework of completely co-dominant, single-locus DNA markers for molecular
breeding. The use of a fluorescent-tailed primer technique resulted in a considerable cost saving. Furthermore, the SSR markers can be multiplexed through optimization, in order to avoid undesirable primer-primer interactions and non-specific amplification. First stage iso-electric focusing of total protein extracts were used to analyze sunflower looking at genetic purity and genetic variety verification on diverse sunflower germplasm. Severe visual interference was visible on most seed storage protein extracts of sunflower. This interference was visible as a distortion in the gel matrix on the anodal end of the gel, and caused important proteins to denature in the presence of heightened field strength and the absence of a uniform matrix. Adjustment of the extraction solutions removed this interference. Total protein profiles were generated with the use ultrathin layer iso-electric focusing (UTLIEF) to assess the level of genetic diversity on the same set of sunflower lines used for the SSR analysis. Finally, the genetic diversity of the sunflower germplasm was analysed by comparing proteomic, genomic and pedigree data from the same germplasm. A total of 295 alleles were amplified with a set of 73 SSR markers with known mapped positions. These were utilized to determine the genetic relatedness of a group of B-lines and R-lines of sunflower.…
Advisors/Committee Members: Laing, Mark D (advisor).
Subjects/Keywords: Sunflowers – Genetics.;
Sunflowers – Breeding.;
Plant molecular genetics.;
Genetic markers.;
Crops – Genetics.;
Plant breeding – Research.;
Sunflowers – Molecular genetics.;
Plant genetics.;
Plant breeding.
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APA (6th Edition):
author], [. (2008). Genetic diversity of proprietary inbred lines of sunflower, determined by mapped SSR markers and total protein analysis.
(Thesis). University of KwaZulu-Natal. Retrieved from http://hdl.handle.net/10413/733
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
author], [No. “Genetic diversity of proprietary inbred lines of sunflower, determined by mapped SSR markers and total protein analysis.
” 2008. Thesis, University of KwaZulu-Natal. Accessed September 17, 2019.
http://hdl.handle.net/10413/733.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
author], [No. “Genetic diversity of proprietary inbred lines of sunflower, determined by mapped SSR markers and total protein analysis.
” 2008. Web. 17 Sep 2019.
Vancouver:
author] [. Genetic diversity of proprietary inbred lines of sunflower, determined by mapped SSR markers and total protein analysis.
[Internet] [Thesis]. University of KwaZulu-Natal; 2008. [cited 2019 Sep 17].
Available from: http://hdl.handle.net/10413/733.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
author] [. Genetic diversity of proprietary inbred lines of sunflower, determined by mapped SSR markers and total protein analysis.
[Thesis]. University of KwaZulu-Natal; 2008. Available from: http://hdl.handle.net/10413/733
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
University of Tennessee – Knoxville
11.
Smallwood, Christopher Joseph.
Molecular Breeding Strategies for Improvement of Complex Traits in Soybean.
Degree: 2015, University of Tennessee – Knoxville
URL: https://trace.tennessee.edu/utk_graddiss/3610
► Soybean [Glycine max (L.) Merrill] is the leading oilseed crop grown in the world. Yield, fatty acids, protein, and oil are commercially important soybean traits;…
(more)
▼ Soybean [Glycine max (L.) Merrill] is the leading oilseed crop grown in the world. Yield, fatty acids, protein, and oil are commercially important soybean traits; thus evaluation of breeding strategies for improvement of these traits is merited. To accomplish this, a comparison of molecular and phenotypic breeding strategies from progeny row selections was performed. From this it was determined that molecular strategies consistently outperformed phenotypic selections (PS) in the progeny row stage for soybean yield, fatty acids, protein, and oil. For yield, Epistacy was the preferred selection method. For fatty acids, protein, and oil, the genomic selection (GS) strategies were preferred. Additionally, a second comparison of molecular and phenotypic strategies was performed with selections from replicated field trials. These comparisons displayed mixed results except for yield, for which PS was the dominant method. With selection from replicated field trials, PS and GS methods were comparable for fatty acids, protein, and oil; indicating that either of these methods could be useful for making improvements. In addition to selection method evaluation, increased knowledge of genomic regions governing soybean yield, fatty acids, protein, and oil would be helpful. Thus, quantitative trait loci (QTL) detection was performed for these traits, with a total of 29 identified. Of these QTLs, three were candidates for confirmed status and four were candidates for positional confirmations. Additionally, possible candidate genes for soybean yield, fatty acids, protein and oil associated with QTLs in this study were identified; as were pleiotropic effects between protein and oil and between the fatty acids. The results from this research should be beneficial for those seeking to make soybean improvements. Researchers making selections from both progeny rows and replicated field trials can draw from these results when choosing which selection strategy to use. The gained knowledge of influential genomic regions for these traits can have application in improvement efforts. Future research seeking to implement high performing molecular breeding strategies and to identify causative genes for vi these and other QTLs impacting targeted traits will be important for the soybean breeding community.
Subjects/Keywords: soybean; plant breeding; genomic selection; quantitative trait; molecular breeding; fatty acid; Plant Breeding and Genetics
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APA (6th Edition):
Smallwood, C. J. (2015). Molecular Breeding Strategies for Improvement of Complex Traits in Soybean. (Doctoral Dissertation). University of Tennessee – Knoxville. Retrieved from https://trace.tennessee.edu/utk_graddiss/3610
Chicago Manual of Style (16th Edition):
Smallwood, Christopher Joseph. “Molecular Breeding Strategies for Improvement of Complex Traits in Soybean.” 2015. Doctoral Dissertation, University of Tennessee – Knoxville. Accessed September 17, 2019.
https://trace.tennessee.edu/utk_graddiss/3610.
MLA Handbook (7th Edition):
Smallwood, Christopher Joseph. “Molecular Breeding Strategies for Improvement of Complex Traits in Soybean.” 2015. Web. 17 Sep 2019.
Vancouver:
Smallwood CJ. Molecular Breeding Strategies for Improvement of Complex Traits in Soybean. [Internet] [Doctoral dissertation]. University of Tennessee – Knoxville; 2015. [cited 2019 Sep 17].
Available from: https://trace.tennessee.edu/utk_graddiss/3610.
Council of Science Editors:
Smallwood CJ. Molecular Breeding Strategies for Improvement of Complex Traits in Soybean. [Doctoral Dissertation]. University of Tennessee – Knoxville; 2015. Available from: https://trace.tennessee.edu/utk_graddiss/3610
12.
Tripathi, Rajiv Kumar.
Genetical studies for agronomic and quality traits in
Indian mustard; -.
Degree: genetics, 2000, Bundelkhand University
URL: http://shodhganga.inflibnet.ac.in/handle/10603/12525
None
Bibliography p.106-128
Advisors/Committee Members: Dabral, R K.Subjects/Keywords: Genetics; Plant Breeding
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APA (6th Edition):
Tripathi, R. K. (2000). Genetical studies for agronomic and quality traits in
Indian mustard; -. (Thesis). Bundelkhand University. Retrieved from http://shodhganga.inflibnet.ac.in/handle/10603/12525
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Tripathi, Rajiv Kumar. “Genetical studies for agronomic and quality traits in
Indian mustard; -.” 2000. Thesis, Bundelkhand University. Accessed September 17, 2019.
http://shodhganga.inflibnet.ac.in/handle/10603/12525.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Tripathi, Rajiv Kumar. “Genetical studies for agronomic and quality traits in
Indian mustard; -.” 2000. Web. 17 Sep 2019.
Vancouver:
Tripathi RK. Genetical studies for agronomic and quality traits in
Indian mustard; -. [Internet] [Thesis]. Bundelkhand University; 2000. [cited 2019 Sep 17].
Available from: http://shodhganga.inflibnet.ac.in/handle/10603/12525.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Tripathi RK. Genetical studies for agronomic and quality traits in
Indian mustard; -. [Thesis]. Bundelkhand University; 2000. Available from: http://shodhganga.inflibnet.ac.in/handle/10603/12525
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
University of Zambia
13.
Nkhoma, Nelia.
Stability of yield and Antioxidant content of selected advanced Cowpea(Vigna unguiculata [L]Walp.) mutation derived lines
.
Degree: 2014, University of Zambia
URL: http://hdl.handle.net/123456789/3413
► Zambia has a number of cowpea germplasm which are high yielding and contain antioxidants which are useful for preventing a lot of health problems i.e.…
(more)
▼ Zambia has a number of cowpea germplasm which are high yielding and contain antioxidants which are useful for preventing a lot of health problems i.e. Heart attack, Hypertension, Obesity and Cancer. Unfortunately the production suitability of these germplasm in the different areas of the country is not known. Based on this, a study was conducted to evaluate the stability of cowpea yield and antioxidants (total phenolic content and condensed tannin content) in the three agro ecological zones as well as to determine the relationship between the antioxidants and the seed coat colour of the cowpeas. Multilocation field trials involving ten cowpea genotypes were conducted at three different Agro-ecological Regions. A randomized complete block design was employed with 3 replications. Cowpea grain yield and antioxidant contents of the seed were determined and a stability bases analysis tool, Additive Main effect and Multiplicative Model (AMMI) was employed for data analysis. Assessment of genotype x environment (GxE) interaction on cowpea grain yield stability indicated that GxE was not present for yield indicating that genotypes did not respond differently to varying environmental conditions. However, some genotypes had higher yields than others indicating genotype identification to specific environments. Genotypes MS1/8/1/4 and LT11/3/3/12 were adapted to high potential yielding environment and were unstable while BB4/2/4/1 and LT11/5/2/2 were adapted to low yielding environment and were stable environments. Assessment for antioxidants showed that GxE was significant (p<0.01) and higher yielding genotypes had low antioxidant contents compared to low yielding genotypes. Genotype LT PRT had higher antioxidant concentration (3.47mg/100mgCE) and stable (IPCA2 0.022) while MS PRT had lower concentrations (0.17mg/100mgCE) and unstable (IPCA1 0.630). The genotypes which had higher antioxidant concentrations had darker seed coat colour (yellowish brown and purplish brown) compared to the ones which had low antioxidant concentration (white). This study identified stable genotypes in both yield and antioxidants. However, further studies for assessing yield stability are necessary and could be achieved by including more seasons and sites to get a better understanding of GxE and yield stability of cowpea in Zambia.
Subjects/Keywords: Cowpea-Germplasm Resources;
Plant Breeding;
Cowpea-Genetics
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APA (6th Edition):
Nkhoma, N. (2014). Stability of yield and Antioxidant content of selected advanced Cowpea(Vigna unguiculata [L]Walp.) mutation derived lines
. (Thesis). University of Zambia. Retrieved from http://hdl.handle.net/123456789/3413
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Nkhoma, Nelia. “Stability of yield and Antioxidant content of selected advanced Cowpea(Vigna unguiculata [L]Walp.) mutation derived lines
.” 2014. Thesis, University of Zambia. Accessed September 17, 2019.
http://hdl.handle.net/123456789/3413.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Nkhoma, Nelia. “Stability of yield and Antioxidant content of selected advanced Cowpea(Vigna unguiculata [L]Walp.) mutation derived lines
.” 2014. Web. 17 Sep 2019.
Vancouver:
Nkhoma N. Stability of yield and Antioxidant content of selected advanced Cowpea(Vigna unguiculata [L]Walp.) mutation derived lines
. [Internet] [Thesis]. University of Zambia; 2014. [cited 2019 Sep 17].
Available from: http://hdl.handle.net/123456789/3413.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Nkhoma N. Stability of yield and Antioxidant content of selected advanced Cowpea(Vigna unguiculata [L]Walp.) mutation derived lines
. [Thesis]. University of Zambia; 2014. Available from: http://hdl.handle.net/123456789/3413
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
University of Manitoba
14.
Asselin, Sean Robert.
Seed quality improvement in the ogu-INRA CMS system in Oilseed Rape (Brassica napus L.).
Degree: Plant Science, 2012, University of Manitoba
URL: http://hdl.handle.net/1993/8363
► The ogu-INRA cytoplasmic male sterility (CMS) system is the global leader for the development of high quality hybrid canola and high erucic acid rapeseed (HEAR)…
(more)
▼ The ogu-INRA cytoplasmic male sterility (CMS) system is the global leader for the development of high quality hybrid canola and high erucic acid rapeseed (HEAR) cultivars of oilseed rape (Brassica napus L.). The largest challenge for
plant breeders using this system is the development of high quality restorer lines (R-lines) due to tight linkage of the radish (Raphanus sativus L.) derived restorer gene PPR-B and elevated seed glucosinolate concentration.
The purpose of this study was to identify improved quality restorer lines for hybrid cultivar development through both field studies and molecular marker development. In the first study 67 R-lines of different genetic backgrounds were screened over the course of two growing seasons and lines with significantly reduced glucosinolate concentration were identified. In the second study a new sequence characterized amplified region (SCAR) marker was successfully developed for the rapid screening of selections for the ogu-INRA CMS restorer gene PPR-B.
Advisors/Committee Members: McVetty, Peter B.E. (Plant Science) (supervisor), Crow, Gary (Animal Science) Li, Genyi (Plant Science) (examiningcommittee).
Subjects/Keywords: Plant Breeding; Genetics; Seed quality; Agronomy
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APA (6th Edition):
Asselin, S. R. (2012). Seed quality improvement in the ogu-INRA CMS system in Oilseed Rape (Brassica napus L.). (Masters Thesis). University of Manitoba. Retrieved from http://hdl.handle.net/1993/8363
Chicago Manual of Style (16th Edition):
Asselin, Sean Robert. “Seed quality improvement in the ogu-INRA CMS system in Oilseed Rape (Brassica napus L.).” 2012. Masters Thesis, University of Manitoba. Accessed September 17, 2019.
http://hdl.handle.net/1993/8363.
MLA Handbook (7th Edition):
Asselin, Sean Robert. “Seed quality improvement in the ogu-INRA CMS system in Oilseed Rape (Brassica napus L.).” 2012. Web. 17 Sep 2019.
Vancouver:
Asselin SR. Seed quality improvement in the ogu-INRA CMS system in Oilseed Rape (Brassica napus L.). [Internet] [Masters thesis]. University of Manitoba; 2012. [cited 2019 Sep 17].
Available from: http://hdl.handle.net/1993/8363.
Council of Science Editors:
Asselin SR. Seed quality improvement in the ogu-INRA CMS system in Oilseed Rape (Brassica napus L.). [Masters Thesis]. University of Manitoba; 2012. Available from: http://hdl.handle.net/1993/8363
Rutgers University
15.
Grimshaw, Austin Lee, 1988-.
Evaluation and breeding of fine fescues for low maintenance applications.
Degree: PhD, Fine fescue, 2019, Rutgers University
URL: https://rucore.libraries.rutgers.edu/rutgers-lib/60736/
► Fine fescues (Festuca spp.) are being bred for low-maintenance turfgrass applications. One of the major limitations to the widespread use of fine fescue is summer…
(more)
▼ Fine fescues (Festuca spp.) are being bred for low-maintenance turfgrass applications. One of the major limitations to the widespread use of fine fescue is summer patch susceptibility and traffic tolerance. Magnaporthiopsis poae (Landschoot & Jackson), is the long known causal organism of summer patch, however recent research has found a new species, Magnaporthiopsis meyeri-festucae (Luo & Zhang) from the diseased roots of fine fescue turfgrasses exhibiting summer patch symptoms.
Breeding for improved tolerance to summer patch is critical but in order to do so a better understanding of the pathogen(s) is necessary. During 2017 and 2018, isolates of M. meyeri-festucae were compared to isolates of M. poae through
plant-fungal interaction in growth chamber experiments and in vitro fungicide sensitivity assays with penthiopyrad, azoxystrobin, and metconazole. In the
plant-fungal interaction experiments, M. poae was shown to exhibit higher levels of virulence than M. meyeri-festucae; however, certain isolates of the two species were ranked equal. In the fungicide sensitivity assays, an isolate of M. meyeri-festucae was shown to be 9.5 times more tolerant to azoxystrobin than a M. poae isolate. These results indicate that M. meyeri-festucae may be involved with summer patch symptoms of fine fescue under field conditions and should be considered along with M. poae when
breeding for tolerance and developing best management strategies for controlling summer patch disease in fine fescue.
Genetic resistance is an important control strategy and could reduce fungicide use. This study determined narrow-sense heritability of summer patch resistance in hard fescue (F. brevilipa R. Tracey) and evaluated inheritance characteristics of summer patch disease resistance. Inheritance characteristics such as heterosis were determined by evaluating the disease severity of progeny from crosses between resistant and susceptible hard fescue clones. Parental clones and progenies from crosses were established in a field trial in a randomized complete block design and inoculated with an isolate of both M. poae and M. meyeri-festucae applied at a rate of 3 cc per
plant of prepared inoculum. Differences in progeny means between crosses were observed. Progeny from resistant × resistant crosses had less disease severity than resistant × susceptible and susceptible × susceptible crosses. Medium narrow-sense heritability estimates support the idea that additive gene action plays a role in disease resistance and that summer patch resistance is possibly quantitatively inherited.
To better understand the tolerance to wear and traffic replicated field studies were established in North Brunswick, NJ and St. Paul, MN, and each included 157 Chewings fescue (F. rubra ssp. commutata Gaudin; syn. F. rubra ssp. fallax (Thuill.) Nyman), 155 hard fescue, and 149 strong creeping red fescue (F. rubra L. rubra Gaudin) genotypes. Wear tolerance was evaluated in North Brunswick and traffic tolerance was evaluated in St. Paul during 2015 and 2016 using different…
Advisors/Committee Members: Bonos, Stacy (chair), Meyer, William (internal member), Zhang, Ning (internal member), Watkins, Eric (outside member), School of Graduate Studies.
Subjects/Keywords: Plant Biology; Fescue – Breeding; Fescue – Genetics
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APA (6th Edition):
Grimshaw, Austin Lee, 1. (2019). Evaluation and breeding of fine fescues for low maintenance applications. (Doctoral Dissertation). Rutgers University. Retrieved from https://rucore.libraries.rutgers.edu/rutgers-lib/60736/
Chicago Manual of Style (16th Edition):
Grimshaw, Austin Lee, 1988-. “Evaluation and breeding of fine fescues for low maintenance applications.” 2019. Doctoral Dissertation, Rutgers University. Accessed September 17, 2019.
https://rucore.libraries.rutgers.edu/rutgers-lib/60736/.
MLA Handbook (7th Edition):
Grimshaw, Austin Lee, 1988-. “Evaluation and breeding of fine fescues for low maintenance applications.” 2019. Web. 17 Sep 2019.
Vancouver:
Grimshaw, Austin Lee 1. Evaluation and breeding of fine fescues for low maintenance applications. [Internet] [Doctoral dissertation]. Rutgers University; 2019. [cited 2019 Sep 17].
Available from: https://rucore.libraries.rutgers.edu/rutgers-lib/60736/.
Council of Science Editors:
Grimshaw, Austin Lee 1. Evaluation and breeding of fine fescues for low maintenance applications. [Doctoral Dissertation]. Rutgers University; 2019. Available from: https://rucore.libraries.rutgers.edu/rutgers-lib/60736/
University of Arkansas
16.
Hummer, Wade Stiles.
Linkage Mapping for Soybean (Glycine Max) Flood Tolerance.
Degree: MS, 2018, University of Arkansas
URL: https://scholarworks.uark.edu/etd/3074
► Flood tolerance in soybean (Glycine max) is not a well-characterized trait, yet flooding damage is second only to drought stress in terms of yield…
(more)
▼ Flood tolerance in soybean (Glycine max) is not a well-characterized trait, yet flooding damage is second only to drought stress in terms of yield reduction. The objectives of this study were to determine genetic variation for flooding tolerance in two populations of soybean recombinant inbred lines (RILs) and to identify and confirm flood tolerant QTL. Population A (WHA) consisted of 111 RILs derived from the cross 5002T by 91210-350 and Population B (WHB) consisted of 79 RILs from the cross RA-452 by Osage. Experiments were conducted at the Rice Research and Extension Center (RREC) near Stuttgart, AR in 2015 and 2016. Flood damage score (FDS) was rated at three, six, and nine days after the flooding treatment was drained. In addition to FDS,
plant population was measured before and after the flooding treatment to calculate the percentage of surviving plants (PS). In 2015, Chlorophyll content was measured using a SPAD meter and normalized difference vegetative index (NDVI) was calculated in 2016. Both RIL populations and parents were genotyped using the SoySNP 6k beadchip with 908 and 1,466 polymorphic markers detected in WHA and WHB, respectively. Twenty nine QTL were identified, five of which validate prior studies involving soybean flood tolerance. Logarithm of Odds (LOD) values from ranged from 3.03 to 6.56 with R squared values ranging from 0.09 - 0.29. A QTL was identified on chromosome 7 that was stable across both WHA and WHB. In population WHA, this QTL was associated with both AUFPC and FDS and explained 9-10% of phenotypic variance. In population WHB, this QTL was associated with PS and explained 15% of phenotypic variance. The results of this study will aid in future development of flood-tolerant soybean germplasm and cultivars by validating previously reported QTL and demonstrating the usefulness of instruments in evaluating flood-tolerance.
Advisors/Committee Members: Richard E. Mason, Pengyin Chen, Kristofer Brye.
Subjects/Keywords: Agronomy; Genetics; Plant Breeding; Soybean; Agronomy and Crop Sciences; Plant Breeding and Genetics
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APA (6th Edition):
Hummer, W. S. (2018). Linkage Mapping for Soybean (Glycine Max) Flood Tolerance. (Masters Thesis). University of Arkansas. Retrieved from https://scholarworks.uark.edu/etd/3074
Chicago Manual of Style (16th Edition):
Hummer, Wade Stiles. “Linkage Mapping for Soybean (Glycine Max) Flood Tolerance.” 2018. Masters Thesis, University of Arkansas. Accessed September 17, 2019.
https://scholarworks.uark.edu/etd/3074.
MLA Handbook (7th Edition):
Hummer, Wade Stiles. “Linkage Mapping for Soybean (Glycine Max) Flood Tolerance.” 2018. Web. 17 Sep 2019.
Vancouver:
Hummer WS. Linkage Mapping for Soybean (Glycine Max) Flood Tolerance. [Internet] [Masters thesis]. University of Arkansas; 2018. [cited 2019 Sep 17].
Available from: https://scholarworks.uark.edu/etd/3074.
Council of Science Editors:
Hummer WS. Linkage Mapping for Soybean (Glycine Max) Flood Tolerance. [Masters Thesis]. University of Arkansas; 2018. Available from: https://scholarworks.uark.edu/etd/3074
University of Arkansas
17.
Salgado Rojas, Alejandra Andrea.
Applying Molecular and Phenotypic Tools to Characterize Flesh Texture and Acidity Traits in the Arkansas Peach Breeding Program and Understanding the Crispy Texture in the Arkansas Blackberry Breeding Program.
Degree: PhD, 2015, University of Arkansas
URL: https://scholarworks.uark.edu/etd/1346
► Quantitative trait loci (QTL) utilizing a pedigree-based analysis (PBA) approach was performed in the peach breeding program for the first time. The pedigree consisted…
(more)
▼ Quantitative trait loci (QTL) utilizing a pedigree-based analysis (PBA) approach was performed in the peach
breeding program for the first time. The pedigree consisted of seven-F1 populations, their parents, ancestors, and cultivars. Flesh firmness and titratable acidity (TA) were examined in 2011, 2012, and 2013. For TA, a consistent and strong QTL was identified on the proximal end of linkage group (LG) 5 of the peach genome. For flesh firmness, two QTLs were located on LG 4. The first QTL was located on the chromosomal region where the slow-melting flesh (SMF) DNA test was identified, and the second QTL was identified in the region of the endopolygalacturonase (endoPG) gene. However, the QTLs on LG 4 were not always consistent. These results indicate that PBA approach for QTL analysis can be applied with success in this mature and ongoing peach
breeding program with the aim to find molecular markers associated with relevant quality traits, which is the first step to apply marker-assisted
breeding (MAB). Also, DNA tests associated with TA and flesh firmness were analyzed with data taken in 2013 and 2014 on this pedigree and other seedlings, selections, and cultivars to predict acidity levels and flesh texture with the final goal to validate these DNA tests and apply MAB. These tests were able to predict correctly the expected acidity levels and flesh textures of the tested individuals.
Firmness and texture are critical traits in blackberry for breeders, growers, and consumers. Crispy and extremely firm fruits were characterized for two blackberry selections which had been observed to have low color reversion (a postharvest disorder). Firmness of these selections and its seedlings were tested in 2013 and 2014. Results indicated that crispy selections had superior firmness and a higher postharvest storage potential compared to cultivars and other selections in the program. These results are important, because firmness will be increased and color reversion will be reduced by use of this germplasm. This research also contributed to better understanding of the physical aspects of crispy and non-crispy genotypes, providing more information about this aspect of blackberries.
Advisors/Committee Members: John R. Clark, Maria Elena Garcia, Richard E. Mason.
Subjects/Keywords: Biological sciences; Fruit crops; Genetics; Plant breeding; Fruit Science; Plant Breeding and Genetics
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APA (6th Edition):
Salgado Rojas, A. A. (2015). Applying Molecular and Phenotypic Tools to Characterize Flesh Texture and Acidity Traits in the Arkansas Peach Breeding Program and Understanding the Crispy Texture in the Arkansas Blackberry Breeding Program. (Doctoral Dissertation). University of Arkansas. Retrieved from https://scholarworks.uark.edu/etd/1346
Chicago Manual of Style (16th Edition):
Salgado Rojas, Alejandra Andrea. “Applying Molecular and Phenotypic Tools to Characterize Flesh Texture and Acidity Traits in the Arkansas Peach Breeding Program and Understanding the Crispy Texture in the Arkansas Blackberry Breeding Program.” 2015. Doctoral Dissertation, University of Arkansas. Accessed September 17, 2019.
https://scholarworks.uark.edu/etd/1346.
MLA Handbook (7th Edition):
Salgado Rojas, Alejandra Andrea. “Applying Molecular and Phenotypic Tools to Characterize Flesh Texture and Acidity Traits in the Arkansas Peach Breeding Program and Understanding the Crispy Texture in the Arkansas Blackberry Breeding Program.” 2015. Web. 17 Sep 2019.
Vancouver:
Salgado Rojas AA. Applying Molecular and Phenotypic Tools to Characterize Flesh Texture and Acidity Traits in the Arkansas Peach Breeding Program and Understanding the Crispy Texture in the Arkansas Blackberry Breeding Program. [Internet] [Doctoral dissertation]. University of Arkansas; 2015. [cited 2019 Sep 17].
Available from: https://scholarworks.uark.edu/etd/1346.
Council of Science Editors:
Salgado Rojas AA. Applying Molecular and Phenotypic Tools to Characterize Flesh Texture and Acidity Traits in the Arkansas Peach Breeding Program and Understanding the Crispy Texture in the Arkansas Blackberry Breeding Program. [Doctoral Dissertation]. University of Arkansas; 2015. Available from: https://scholarworks.uark.edu/etd/1346
18.
Bhusal, Siddhi Jeewan.
Evaluation and Identification of Soybean Aphid Resistance Sources and Mapping of Soybean Aphid Resistance Loci in Early Maturing Soybean Germplasm Accessions.
Degree: PhD, Plant Science, 2016, South Dakota State University
URL: http://openprairie.sdstate.edu/etd/1007
► Soybean aphid (Aphis glycines Matsumura) has been a major pest of soybean [Glycine max (L.) Merr.] in North America particularly in the northern United…
(more)
▼ Soybean aphid (Aphis glycines Matsumura) has been a major pest of soybean [Glycine max (L.) Merr.] in North America particularly in the northern United States and three Canadian provinces. At least four biotypes of soybean aphid have been confirmed in the United States. Identification of soybean aphid resistance sources in early-maturing soybeans and genetic characterization of new sources of resistance will facilitate to expand the gene pool of soybean aphid resistance and thus will help to develop soybean aphid resistant cultivars. To identify new sources of resistance in early maturing soybeans, 330 soybean germplasm accessions from Maturity Group (MG) I, along with 11 resistant or susceptible checks, were evaluated against soybean aphid in greenhouse by caged (no-choice) and non-caged tests. Germplasm accessions that showed resistance in these tests were then tested in the field under natural infestations supplemented by artificial infestation. In the greenhouse tests, accessions PI 189946, PI 153214, and PI 437075 exhibited low SA levels (test) similar to those on resistant checks. Six accessions (PI 378663, PI 603587A, PI 567250A, PI 603326, PI 603339A, and PI 603546A) showed moderate aphid resistance withfield, PI 567250A and PI 603339A performed similar to resistant checks, and PI 153214 and PI 437075 showed moderate aphid resistance. Across all three tests, PI 567250A and PI 603339A exhibited consistently low levels of aphid infestation and thus may be useful in soybean
breeding as newly identified sources of SA resistance. To characterize the genetic basis of soybean aphid resistance in PI 603712, a newly identified resistant gemplasm line, 142 F2 plants derived from the cross ‘Roberts’ x PI 603712 and their parents were evaluated for soybean aphid resistance in the greenhouse, and were genotyped with BARCSoySNP6K Illumina Infinium II BeadChip. A genome-wide molecular linkage map was constructed with 1495 polymorphic SNP markers. QTL analysis revealed that PI 603712 possessed two major loci of soybean aphid resistance, which were located on chromosome 7 and 16, respectively. The locus on chromosome 7 was dominantly expressed and positioned about one Mega-base-pair (Mb) far from the previously identified resistance locus Rag1. The locus on chromosome 16 was partially dominant and positioned near the previously identified resistance locus Rag3. Interestingly, two minor loci for susceptibility were also detected on chromosomes 13 and 17 in PI 603712. In developing soybean aphid resistant cultivars through marker-assisted selection, an appropriate combination of resistance loci should be selected when PI 603712 is used as a donor parent of resistance. To dissect the genetic architecture of soybean aphid resistance, genome-wide association study (GWAS) was conducted in a population consisting of 330 early maturing germplasm accessions which were genotyped with the Illumina Infinium SoySNP50K BeadChip. Phenotyping was performed in the USDA-ARS Eastern South Dakota Soil and Water Research Farm near Brookings in…
Advisors/Committee Members: Jose L. Gonzalez-Hernandez.
Subjects/Keywords: Plant Biology; Plant Breeding and Genetics; Plant Sciences
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Export
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APA (6th Edition):
Bhusal, S. J. (2016). Evaluation and Identification of Soybean Aphid Resistance Sources and Mapping of Soybean Aphid Resistance Loci in Early Maturing Soybean Germplasm Accessions. (Doctoral Dissertation). South Dakota State University. Retrieved from http://openprairie.sdstate.edu/etd/1007
Chicago Manual of Style (16th Edition):
Bhusal, Siddhi Jeewan. “Evaluation and Identification of Soybean Aphid Resistance Sources and Mapping of Soybean Aphid Resistance Loci in Early Maturing Soybean Germplasm Accessions.” 2016. Doctoral Dissertation, South Dakota State University. Accessed September 17, 2019.
http://openprairie.sdstate.edu/etd/1007.
MLA Handbook (7th Edition):
Bhusal, Siddhi Jeewan. “Evaluation and Identification of Soybean Aphid Resistance Sources and Mapping of Soybean Aphid Resistance Loci in Early Maturing Soybean Germplasm Accessions.” 2016. Web. 17 Sep 2019.
Vancouver:
Bhusal SJ. Evaluation and Identification of Soybean Aphid Resistance Sources and Mapping of Soybean Aphid Resistance Loci in Early Maturing Soybean Germplasm Accessions. [Internet] [Doctoral dissertation]. South Dakota State University; 2016. [cited 2019 Sep 17].
Available from: http://openprairie.sdstate.edu/etd/1007.
Council of Science Editors:
Bhusal SJ. Evaluation and Identification of Soybean Aphid Resistance Sources and Mapping of Soybean Aphid Resistance Loci in Early Maturing Soybean Germplasm Accessions. [Doctoral Dissertation]. South Dakota State University; 2016. Available from: http://openprairie.sdstate.edu/etd/1007
Wilfrid Laurier University
19.
Clemow, Scott.
Insights Into The World of Pea Nodulation Using the Low Nodulator R50.
Degree: 2010, Wilfrid Laurier University
URL: https://scholars.wlu.ca/etd/1019
► Cytokinin oxidase (CKX) is the enzyme responsible for the degradation of cytokinin, a class of adenine-based plant hormones that stimulate cell division, among other physiological…
(more)
▼ Cytokinin oxidase (CKX) is the enzyme responsible for the degradation of cytokinin, a class of adenine-based plant hormones that stimulate cell division, among other physiological processes. The pea mutant R50 is characterized by having a pale leaf phenotype, dwarf stature, few lateral roots, low nodule formation, and elevated levels of endogenous cytokinins in its shoots, roots and nodules. When compared to that of the wild-type Sparkle, total CKX activity is low but the transcript levels of PsCKX1 are significantly higher. In this study, I investigated the expression of PsCKX1 throughout the development of a nodule and the localization of PsCKX1 in the nodules of Sparkle and R50. To achieve this goal, a spot-inoculation technique was developed to capture different stages of nodule organogenesis. Sparkle nodules developed as expected. The nodules of R50, however, were delayed with root hairs exhibiting abnormal waving. Furthermore, an accumulation of starch grains was noticeable in the mature nodules which formed. Transcription of PsCKX1 was up-regulated upon inoculation, reaching a plateau for Sparkle but continuously increasing for R50. There were no differences in the localization of PsCKX1 between the two lines, but the immunofluorescence signal was much higher in the mutant. Finally, a protocol for the creation of composite plants was worked out with the goal of using the technique for later complementation of R50. All evidence allowed me to suggest that R50 produces a defective PsCKX1 protein which maybe the cause of abnormal nodulation.
Subjects/Keywords: Plant Biology; Plant Breeding and Genetics; Plant Sciences
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MLA ·
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CSE |
Export
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APA (6th Edition):
Clemow, S. (2010). Insights Into The World of Pea Nodulation Using the Low Nodulator R50. (Thesis). Wilfrid Laurier University. Retrieved from https://scholars.wlu.ca/etd/1019
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Clemow, Scott. “Insights Into The World of Pea Nodulation Using the Low Nodulator R50.” 2010. Thesis, Wilfrid Laurier University. Accessed September 17, 2019.
https://scholars.wlu.ca/etd/1019.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Clemow, Scott. “Insights Into The World of Pea Nodulation Using the Low Nodulator R50.” 2010. Web. 17 Sep 2019.
Vancouver:
Clemow S. Insights Into The World of Pea Nodulation Using the Low Nodulator R50. [Internet] [Thesis]. Wilfrid Laurier University; 2010. [cited 2019 Sep 17].
Available from: https://scholars.wlu.ca/etd/1019.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Clemow S. Insights Into The World of Pea Nodulation Using the Low Nodulator R50. [Thesis]. Wilfrid Laurier University; 2010. Available from: https://scholars.wlu.ca/etd/1019
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
University of Tennessee – Knoxville
20.
Hughes, Eifion Wyn.
Genetic Variability for Biomass Yield, Yield Components, and Ethanol Yield among Half-sibs of Switchgrass.
Degree: MS, Plant Sciences, 2014, University of Tennessee – Knoxville
URL: https://trace.tennessee.edu/utk_gradthes/2725
► For bioenergy crops to be an economical alternative to fossil fuels, rapid biological and technological advancements will need to occur. Some advancements can be…
(more)
▼ For bioenergy crops to be an economical alternative to fossil fuels, rapid biological and technological advancements will need to occur. Some advancements can be accomplished by producing new switchgrass (
Panicum virgatum L.) varieties with higher biomass and ethanol yields. The objective of this research was to quantify biomass and ethanol yield potential of four F
1 [F1] half-sib populations for future variety development as a bioenergy crop.
The four parental lines were PI 421999 (AR), PI 607837 (TX), PI 421552 (Cimarron), and Exp. NSL-2001-1 (OK). Seed for one hundred and forty F
1 [F1] half-sib progeny were produced in a polycross nursery at the East Tennessee Research and Education Center (ETREC),
Plant Sciences Unit, Knoxville. The parents and half-sibs were evaluated at the ETREC, Holston Unit. Evaluations were based on a fall one-cut biomass system in 2010 – 2011 and a fall biomass harvest following a spring forage harvest system in 2012 - 2013. Samples of the above ground biomass harvest for each
plant were collected every year and analyzed for ethanol production. Agronomic trait ratings (
plant height, tiller count, tiller size, leaf angle, leaf width, and bloom score) were conducted each year.
Mean biomass yield was 1.04 kg
plant-1 [
plant-1] for all populations and years, with average biomass yields among populations ranging from 0.57 to 2.12 kg
plant-1 [
plant-1]. Panmictic heterosis was observed in two of the four years (2011 and 2012) of the study. Within family genetic variances for 2010, 2011, 2012, and 2013 ranged from 0 to 0.10, 0.61, 0.44, and 0.06 respectively. Broad-sense heritability values ranged from 0 to a high of 0.78. Correlations were observed between yield and
plant height (r=0.65) and leaf width (r=0.36).
Predicted ethanol yield was 0.27 L ethanol
plant-1 [
plant-1] across years and populations. The highest ethanol yield was 1.32 L
plant-1 [
plant-1]. Mean lignin content was 76 g lignin kg DM
-1 [DM-1]. Data from 2012 indicated greater ethanol yields from stems than from leaves. The leaves contained higher percentages of cellulose (41-42%) than the stems (40-42%), while the stems were comprised of higher percentages of both hemicellulose (43-44%) and lignin (6.0 – 6.1%).
Advisors/Committee Members: Fred L. Allen, Hem S. Bhandari, Dennis R. West, Arnold M. Saxton.
Subjects/Keywords: Switchgrass; Breeding; Ethanol; Biomass; Yield; Heterosis; Plant Breeding and Genetics
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APA ·
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CSE |
Export
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APA (6th Edition):
Hughes, E. W. (2014). Genetic Variability for Biomass Yield, Yield Components, and Ethanol Yield among Half-sibs of Switchgrass. (Thesis). University of Tennessee – Knoxville. Retrieved from https://trace.tennessee.edu/utk_gradthes/2725
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Hughes, Eifion Wyn. “Genetic Variability for Biomass Yield, Yield Components, and Ethanol Yield among Half-sibs of Switchgrass.” 2014. Thesis, University of Tennessee – Knoxville. Accessed September 17, 2019.
https://trace.tennessee.edu/utk_gradthes/2725.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Hughes, Eifion Wyn. “Genetic Variability for Biomass Yield, Yield Components, and Ethanol Yield among Half-sibs of Switchgrass.” 2014. Web. 17 Sep 2019.
Vancouver:
Hughes EW. Genetic Variability for Biomass Yield, Yield Components, and Ethanol Yield among Half-sibs of Switchgrass. [Internet] [Thesis]. University of Tennessee – Knoxville; 2014. [cited 2019 Sep 17].
Available from: https://trace.tennessee.edu/utk_gradthes/2725.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Hughes EW. Genetic Variability for Biomass Yield, Yield Components, and Ethanol Yield among Half-sibs of Switchgrass. [Thesis]. University of Tennessee – Knoxville; 2014. Available from: https://trace.tennessee.edu/utk_gradthes/2725
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
University of Arkansas
21.
Frett, Terrence James.
Genetic Determinism of Xanthomonas arboricola pv. pruni (Xap) Resistance, Fruit Quality, and Phenological Traits in Peach and Incorporation of Marker-Assisted Selection (MAS) in the University of Arkansas Peach and Nectarine Breeding Program.
Degree: PhD, 2016, University of Arkansas
URL: https://scholarworks.uark.edu/etd/1448
► For over two decades the incorporation of marker-assisted selection (MAS) has been discussed as an innovative approach to increase peach breeding efficiency. Although hundreds…
(more)
▼ For over two decades the incorporation of marker-assisted selection (MAS) has been discussed as an innovative approach to increase peach
breeding efficiency. Although hundreds of quantitative trait loci (QTLs) have been identified, only a few have been converted into usable MAS tools. This highlights a disconnect between genomic discoveries and practical application in
breeding programs, which has delayed use of MAS.
In this dissertation, three sequential steps were performed with the objective to bridge this gap and develop
breeding-relevant DNA tests for MAS of fruit bacterial spot resistance (Xap1 and Xap6), fruit quality [blush (Rf), acidity (D), and acidity and soluble solid content (G7Flav)], and phenological traits [maturity date (G4mat), fruit type (G), and flesh color (Y)] across four RosBREED peach
breeding programs. First, previously identified QTLs were further investigated and 32 SNPs were grouped into haplotypes and validated for association with
breeding-relevant trait differences across RosBREED germplasm. The SNPs were divided into two mini-arrays and advanced
breeding material from all programs were outsourced for design and testing. The validated SNP loci were used in marker-assisted parent selection (MAPS) in 2013-2015 at the University of Arkansas (UA) program to combine horizontal Xap resistance with high fruit quality spanning the season. Secondly, four of the SNP-based tests (Xap1, Xap6, G, and Y loci) were converted into sequence length polymorphism-based (SLP-based) tests (Ppe-XapF1-SSR, Ppe-XapF6-SSR, indelG, and PpCCD4b-SSR) and screened on the UA RosBREED germplasm and 22 additional F1 populations. Results were compared across both DNA tests to identify the most efficient genotyping approach for each trait. In 2015, two of the SLP-based DNA tests, the indelG (pubescent vs. glabrous) and PpCCD4b-SSR (white vs. yellow flesh) were advanced to test in marker-assisted seedling selection (MASS).
In the final step, QTL analysis was conducted for Xap fruit, Xap leaf, and Xap leaf-assay resistance along with seven fruit quality and phenological traits using the Pedigree-Based Analysis (PBA) approach and the UA RosBREED pedigree. Overall 20 QTLs were identified, 10 for Xap resistance and 10 for quality and phenological traits. These 20 QTLs are optimal targets for future DNA test development, validation, and use in MAS.
Advisors/Committee Members: John R. Clark, Burton H. Bluhm, Elena M. Garcia.
Subjects/Keywords: Biological sciences; Breeding; Disease resistance breeding; Fruit; Horticulture; Marker-assisted breeding; Qtl; Fruit Science; Plant Biology; Plant Breeding and Genetics
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APA ·
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MLA ·
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CSE |
Export
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Manager
APA (6th Edition):
Frett, T. J. (2016). Genetic Determinism of Xanthomonas arboricola pv. pruni (Xap) Resistance, Fruit Quality, and Phenological Traits in Peach and Incorporation of Marker-Assisted Selection (MAS) in the University of Arkansas Peach and Nectarine Breeding Program. (Doctoral Dissertation). University of Arkansas. Retrieved from https://scholarworks.uark.edu/etd/1448
Chicago Manual of Style (16th Edition):
Frett, Terrence James. “Genetic Determinism of Xanthomonas arboricola pv. pruni (Xap) Resistance, Fruit Quality, and Phenological Traits in Peach and Incorporation of Marker-Assisted Selection (MAS) in the University of Arkansas Peach and Nectarine Breeding Program.” 2016. Doctoral Dissertation, University of Arkansas. Accessed September 17, 2019.
https://scholarworks.uark.edu/etd/1448.
MLA Handbook (7th Edition):
Frett, Terrence James. “Genetic Determinism of Xanthomonas arboricola pv. pruni (Xap) Resistance, Fruit Quality, and Phenological Traits in Peach and Incorporation of Marker-Assisted Selection (MAS) in the University of Arkansas Peach and Nectarine Breeding Program.” 2016. Web. 17 Sep 2019.
Vancouver:
Frett TJ. Genetic Determinism of Xanthomonas arboricola pv. pruni (Xap) Resistance, Fruit Quality, and Phenological Traits in Peach and Incorporation of Marker-Assisted Selection (MAS) in the University of Arkansas Peach and Nectarine Breeding Program. [Internet] [Doctoral dissertation]. University of Arkansas; 2016. [cited 2019 Sep 17].
Available from: https://scholarworks.uark.edu/etd/1448.
Council of Science Editors:
Frett TJ. Genetic Determinism of Xanthomonas arboricola pv. pruni (Xap) Resistance, Fruit Quality, and Phenological Traits in Peach and Incorporation of Marker-Assisted Selection (MAS) in the University of Arkansas Peach and Nectarine Breeding Program. [Doctoral Dissertation]. University of Arkansas; 2016. Available from: https://scholarworks.uark.edu/etd/1448
University of Tennessee – Knoxville
22.
Wiggins, Suzannah Joy.
Molecular Marker Assisted Development and Stability Analysis of Low Phytate, High Inorganic Phosphorus Soybean [Glycine max (L.) Merrill].
Degree: MS, Plant Sciences, 2012, University of Tennessee – Knoxville
URL: https://trace.tennessee.edu/utk_gradthes/1221
► Phytate [myo-inositol 1,2,3,4,5,6-hexakisphosphate] is a mixed cation salt of phytic acid which binds to other minerals, in many field crops including soybean [Glycine max…
(more)
▼ Phytate [
myo-inositol 1,2,3,4,5,6-hexa
kisphosphate] is a mixed cation salt of phytic acid which binds to other minerals, in many field crops including soybean [Glycine max (L.) Merr.]. Livestock with monogastric digestive systems lack the ability to break bonds between phytate and these minerals, causing phytate phosphorus (P) to be excreted in the waste and contributing to possible P and other mineral deficiencies. Discovery of single sequence repeat (SSR) and single nucleotide polymorphism (SNP) markers associated with low phytate QTL (cqPha-001 and cqPha-002) have aided in development of low phytate soybean lines. The objectives of this study were to 1) utilize SSRs and SNPs to identify the heterozygous and recessive allelic forms of low phytate loci during trait introgression into a high yielding commercial cultivar; 2) evaluate differences in genotypic values for agronomic and seed quality traits, and seed inorganic phosphorous (Pi) concentration between the recurrent parent 5601T and BC
4, BC
3, and BC
2 derived progeny lines; and 3) evaluate those traits and environmental stability of inorganic P in the recurrent parent 5601T and the BC
4 derived line TN09-239 across ten southern U.S. environments. Successful trait introgression was confirmed in the BC
4 line TN09-239 via SNP assay. In comparison to the recurrent parent 5601T (222.9 ng µL
-1) the BC
4 derived line TN09-239 (1675.9 ng µL
-1) contained significantly higher Pi (P-1 for 5601T and 3151 kg ha
-1 for TN09-239) (P0.05), while TN09-239 had a slope significantly different from zero as well as from one (P
Advisors/Committee Members: Vincent R. Pantalone, Dennis R. West, Dean A. Kopsell, Carl E. Sams.
Subjects/Keywords: Soybean breeding; low phytate; Agricultural Science; Agronomy and Crop Sciences; Genetics; Plant Breeding and Genetics
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APA (6th Edition):
Wiggins, S. J. (2012). Molecular Marker Assisted Development and Stability Analysis of Low Phytate, High Inorganic Phosphorus Soybean [Glycine max (L.) Merrill]. (Thesis). University of Tennessee – Knoxville. Retrieved from https://trace.tennessee.edu/utk_gradthes/1221
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Wiggins, Suzannah Joy. “Molecular Marker Assisted Development and Stability Analysis of Low Phytate, High Inorganic Phosphorus Soybean [Glycine max (L.) Merrill].” 2012. Thesis, University of Tennessee – Knoxville. Accessed September 17, 2019.
https://trace.tennessee.edu/utk_gradthes/1221.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Wiggins, Suzannah Joy. “Molecular Marker Assisted Development and Stability Analysis of Low Phytate, High Inorganic Phosphorus Soybean [Glycine max (L.) Merrill].” 2012. Web. 17 Sep 2019.
Vancouver:
Wiggins SJ. Molecular Marker Assisted Development and Stability Analysis of Low Phytate, High Inorganic Phosphorus Soybean [Glycine max (L.) Merrill]. [Internet] [Thesis]. University of Tennessee – Knoxville; 2012. [cited 2019 Sep 17].
Available from: https://trace.tennessee.edu/utk_gradthes/1221.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Wiggins SJ. Molecular Marker Assisted Development and Stability Analysis of Low Phytate, High Inorganic Phosphorus Soybean [Glycine max (L.) Merrill]. [Thesis]. University of Tennessee – Knoxville; 2012. Available from: https://trace.tennessee.edu/utk_gradthes/1221
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
23.
Dean, Deborah.
Assessing the genetic diversity of the genus <i>Viburnum</i> using simple sequence repeats.
Degree: 2014, University of Tennessee – Knoxville
URL: https://trace.tennessee.edu/utk_graddiss/3118
► The genus Viburnum was established in 1753 by Linnaeus and is the largest genus in the Adoxaceae and consists of approximately 160 species. Viburnum species…
(more)
▼ The genus Viburnum was established in 1753 by Linnaeus and is the largest genus in the Adoxaceae and consists of approximately 160 species. Viburnum species are small trees which grow throughout the temperate regions of the Northern Hemisphere (Hoch, 1995). While this genus shares little variety in fruit and flower morphology, it is diverse in many other traits. As molecular studies advance, this large genus continues to undergo reclassification. Here three SSR libraries were constructed to discern additional molecular insight into this vast genus.
Microsatellite markers were developed to characterize Viburnum on several different levels. V. dilatatum is an introduced Asian species that has a wide range of desirable horticultural traits, but this is countered with a propensity for its seedlings to become invasive, therefore creating an interest in the genetics and breeding of this species. Eleven SSR markers were used to characterize V. dilatatum, and are expected to aid in breeding programs that are attempting to develop new cultivars and assist with early detection and screening of plants that have escaped cultivation.
Genetic diversity and population structure was examined in the native species V. rufidulum. The populations examined were found to have a low to moderate genetic differentiation and high level of gene flow. The greatest genetic variation was found to exist within populations. This coupled with the high cross transferability of the SSR markers to other Viburnum species are expected to be helpful in refining the phylogenetic relationship of Viburnum and other genera in Adoxaceae.
In the past the Viburnum has undergone various reclassifications based on morphological and molecular studies. Thirty-three microsatellite markers were developed from V. dilatatum, V. farreri, and V. rufidulum and utilized to characterize the relationships between various taxa. Cross transferability of the loci was analyzed and the polymorphic information content ranged from 0.42 to 0.97%. Three loci were used to construct a phylogenic tree. Sequence alignments indicated well preserved primer sites and resulted in cross transferability among many Viburnum species and to other genera. We expect this set of SSR markers to of utility in future genetic studies, marker assisted selection, and breeding programs.
Subjects/Keywords: Viburnum; SSR; population genetics; genetic diversity; Plant Breeding and Genetics
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APA (6th Edition):
Dean, D. (2014). Assessing the genetic diversity of the genus <i>Viburnum</i> using simple sequence repeats. (Doctoral Dissertation). University of Tennessee – Knoxville. Retrieved from https://trace.tennessee.edu/utk_graddiss/3118
Chicago Manual of Style (16th Edition):
Dean, Deborah. “Assessing the genetic diversity of the genus <i>Viburnum</i> using simple sequence repeats.” 2014. Doctoral Dissertation, University of Tennessee – Knoxville. Accessed September 17, 2019.
https://trace.tennessee.edu/utk_graddiss/3118.
MLA Handbook (7th Edition):
Dean, Deborah. “Assessing the genetic diversity of the genus <i>Viburnum</i> using simple sequence repeats.” 2014. Web. 17 Sep 2019.
Vancouver:
Dean D. Assessing the genetic diversity of the genus <i>Viburnum</i> using simple sequence repeats. [Internet] [Doctoral dissertation]. University of Tennessee – Knoxville; 2014. [cited 2019 Sep 17].
Available from: https://trace.tennessee.edu/utk_graddiss/3118.
Council of Science Editors:
Dean D. Assessing the genetic diversity of the genus <i>Viburnum</i> using simple sequence repeats. [Doctoral Dissertation]. University of Tennessee – Knoxville; 2014. Available from: https://trace.tennessee.edu/utk_graddiss/3118
Cornell University
24.
ROMERO NAVARRO, JORGE ALBERTO.
Comprehensive characterization of maize landraces: integrative strategies to identify and deploy useful allelic diversity
.
Degree: 2017, Cornell University
URL: http://hdl.handle.net/1813/56994
► The characterization of natural genetic diversity and the exploration of its relationship with variation in phenotypic traits is of great interest for evolutionary, conservation, and…
(more)
▼ The characterization of natural genetic diversity and the exploration of its relationship with variation in phenotypic traits is of great interest for evolutionary, conservation, and improvement purposes; in addition, understanding the relationships between genotype, phenotype, and the environment can provide insight on the molecular pathways controlling quantitative traits, as well as their fitness implications. The following work entails the characterization of a comprehensive panel of maize landraces from Latin America and is divided in 5 sections.
The chapter "Identifying the diamond in the rough: a study of allelic diversity underlying flowering time adaptation in maize landraces" describes the general experimental design used to characterize the landrace panel. In this chapter the relationship between large scale altitude and latitude adaptation is also explored, and the trait flowering time is used as a case study to explore the genetic architecture of a complex trait through field experiments. The chapter "Genome-environment association allows identifying useful adaptive alleles from maize landraces" explores further the relationship between the genotypic variation in landraces and their adaptation to local abiotic environmental conditions. Because landraces have evolved for thousands of years in those environments, we observe significant association at candidate genes and observed that adaptive alleles are common and shared across populations, which has important consequences for future
breeding efforts. The chapter "Genome wide association for
plant height variation in maize landraces" represents the analysis of the genetic basis of
plant height variation in landraces.
Plant height, like yield, is a very complex trait with a significant heritable component. The association at key hormonal regulators, as well as flowering time associated regions, shows the potential to unveil genes underlying this trait, however the results of phenotypic prediction suggest that higher marker density is necessary to study traits on this order of complexity in a panel of very diverse landraces. The chapter "Exploring the potential for finding sources of resistance to Fusarium ear rot among maize landraces" represents the analyses of phenotypic evaluation of inoculated ear rot trials. Lastly, the final chapter "Integration of controlled populations and association mapping to score cytological features in the maize genome" describes the joint analyses of a mapping population segregating for the abnormal chromosome 10 and the landraces accessions. By combining the results of both populations, putative calls are made in the landraces regarding their chromosome 10 allele.
Advisors/Committee Members: Mezey, Jason G. (committeeMember), Smith Einarson, Margaret Elizabeth (committeeMember).
Subjects/Keywords: Landraces;
Maize;
Quantitative Genetics;
Genetics;
Genomics;
Plant Breeding
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APA (6th Edition):
ROMERO NAVARRO, J. A. (2017). Comprehensive characterization of maize landraces: integrative strategies to identify and deploy useful allelic diversity
. (Thesis). Cornell University. Retrieved from http://hdl.handle.net/1813/56994
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
ROMERO NAVARRO, JORGE ALBERTO. “Comprehensive characterization of maize landraces: integrative strategies to identify and deploy useful allelic diversity
.” 2017. Thesis, Cornell University. Accessed September 17, 2019.
http://hdl.handle.net/1813/56994.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
ROMERO NAVARRO, JORGE ALBERTO. “Comprehensive characterization of maize landraces: integrative strategies to identify and deploy useful allelic diversity
.” 2017. Web. 17 Sep 2019.
Vancouver:
ROMERO NAVARRO JA. Comprehensive characterization of maize landraces: integrative strategies to identify and deploy useful allelic diversity
. [Internet] [Thesis]. Cornell University; 2017. [cited 2019 Sep 17].
Available from: http://hdl.handle.net/1813/56994.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
ROMERO NAVARRO JA. Comprehensive characterization of maize landraces: integrative strategies to identify and deploy useful allelic diversity
. [Thesis]. Cornell University; 2017. Available from: http://hdl.handle.net/1813/56994
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
University of Maine
25.
Sun, Youping.
Genetic Diversity, Micro Propagation, and Cold Hardiness of Ilex glabra (L.) A. Gray.
Degree: PhD, Plant Science, 2010, University of Maine
URL: https://digitalcommons.library.umaine.edu/etd/261
► Ilex glabra (L.) A.Gray (inkberry) is a native evergreen shrub with dark green foliage and compact habit. This shrub has gained popularity in the…
(more)
▼ Ilex glabra (L.) A.Gray (inkberry) is a native evergreen shrub with dark green foliage and compact habit. This shrub has gained popularity in the northern landscapes of the United States and more nursery growers would like to produce it. To better understand genetic relationships among inkberry cultivars and breed cold-hardy cultivars for northern nursery growers and landscape specialists, the following projects were conducted. A group of 48 inkberry accessions and two other Ilex species (Ilex crenata Thunb. and I. mutchagara Makino) were studied using amplified fragment length polymorphism (AFLP) markers. A total of 229 markers between 50 and 500 base pairs (bps) were produced from eight AFLP primer combinations. Eighty-seven percent of the markers were polymorphic. The markers for each primer-pair ranged from 22 to 45 and the genetic distance ranged from 0.001 to 0.349. Within the inkberry clade, 48 accessions were classified into six groups including, a wild species group and five cultivated groups, ‘Densa’, f. leucocarpa, ‘Pretty Girl’, ‘Shamrock’ and ‘Viridis’. Within each group, the legitimacy of named cultivars and distinguished clones were discussed based on both morphological and molecular data. Inkberry was hybridized with cold-hardy male Ilex verticillata (L.) A. Gray (common winterberry) and Ilex × meserveae S.Y. Hu (meserve holly) to increase the cold hardiness. Cross pollination of inkberry and its five cultivars with both male plants was carried out in a greenhouse. Inkberry ‘Chamzin’ and ‘Densa’ had higher compatibility with either common winterberry or meserve holly, while inkberry wild species and its cultivar ‘Compacta’ were less compatible, and ‘Nigra’ and ‘Shamrock’ were almost incompatible. The pollen germination in situ observed with fluorescence microscope also supported the above results. Pollen germination of common winterberry and meserve holly on the stigma of inkberry wild species and inkberry ‘Chamzin’, ‘Compacta’, ‘Densa’ was more than that on inkberry ‘Nigra’ and ‘Shamrock’. A dramatic reduction in the number of pollen tubes was observed as they grew along the style and into ovary. The percentage of pollen tubes reaching the ovary of inkberry wild species and inkberry ‘Chamzin’, ‘Compacta’, ‘Densa’ was higher than that of inkberry ‘Nigra’ and ‘Shamrock’. Most of their fruit set were aborted and the fully developed seeds were less than 54.2% (meserve holly) and 32.4% (common winterberry). Reproduction barriers, including the inhibition of pollen germination, pollen tube growth to the style and the ovary, and lack of fertilization, resulted in the cross incompatibility of inkberry with both cold hardy species. Further cross-pollination should consider the incompatibility of cultivar variations. Nodal segments containing one axillary bud (1-1.5 cm) of inkberry were established on a Murashige and Skoog (MS) medium without hormones. The sprouted shoots (~1.0 cm) were cultured on a MS medium supplemented with BAP, KIN or ZT at 2.3, 4.5, 9.1, or…
Advisors/Committee Members: Donglin Zhang, Stephanie Burnett, Michael Day.
Subjects/Keywords: Genetic transformation; Pokeweed; Life Sciences; Plant Breeding and Genetics; Plant Sciences
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APA ·
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APA (6th Edition):
Sun, Y. (2010). Genetic Diversity, Micro Propagation, and Cold Hardiness of Ilex glabra (L.) A. Gray. (Doctoral Dissertation). University of Maine. Retrieved from https://digitalcommons.library.umaine.edu/etd/261
Chicago Manual of Style (16th Edition):
Sun, Youping. “Genetic Diversity, Micro Propagation, and Cold Hardiness of Ilex glabra (L.) A. Gray.” 2010. Doctoral Dissertation, University of Maine. Accessed September 17, 2019.
https://digitalcommons.library.umaine.edu/etd/261.
MLA Handbook (7th Edition):
Sun, Youping. “Genetic Diversity, Micro Propagation, and Cold Hardiness of Ilex glabra (L.) A. Gray.” 2010. Web. 17 Sep 2019.
Vancouver:
Sun Y. Genetic Diversity, Micro Propagation, and Cold Hardiness of Ilex glabra (L.) A. Gray. [Internet] [Doctoral dissertation]. University of Maine; 2010. [cited 2019 Sep 17].
Available from: https://digitalcommons.library.umaine.edu/etd/261.
Council of Science Editors:
Sun Y. Genetic Diversity, Micro Propagation, and Cold Hardiness of Ilex glabra (L.) A. Gray. [Doctoral Dissertation]. University of Maine; 2010. Available from: https://digitalcommons.library.umaine.edu/etd/261
Iowa State University
26.
Ibore, Martha.
Genetic architecture of antibiosis and antixenosis aphid resistance in soybean and elucidation of the molecular effect of pyramiding Rag1 and Rag2 resistance genes.
Degree: 2017, Iowa State University
URL: https://lib.dr.iastate.edu/etd/16517
► Soybean aphids are a major problem to soybean growers worldwide and they cause great yield losses. In the United States, soybean aphids can cause yield…
(more)
▼ Soybean aphids are a major problem to soybean growers worldwide and they cause great yield losses. In the United States, soybean aphids can cause yield losses of up to 50% especially on susceptible varieties. Yield losses attributed to feeding by soybean aphids can be reduced by cultivating resistant soybean varieties. In recent years, certain biotypes of soybean aphids that can colonize resistant soybean plants have been reported, suggesting the continued need for identification of novel sources of aphid resistance to include in plant breeding programs. While gene pyramiding, in which certain resistant soybean genotypes carry more than one aphid resistance gene has been effectively used to mitigate the problem of virulent aphid biotypes on resistant soybean, the molecular mechanisms of increased resistance in soybean genotypes with multiple genes is still unknown. Research presented in this dissertation utilized a genome-wide association study (GWAS) to identify novel sources of soybean aphid resistance and characterize the genetic architecture of aphid resistance in a diverse panel of USDA soybean accessions. Using RNA-seq, the molecular mechanisms of increased resistance that results from pyramiding two aphid-resistance genes (Rag1 and Rag2) in one soybean genotype (Rag1Rag2) were elucidated.
In the GWAS project, we identified significant Single Nucleotide Polymorphisms (SNPs) located in genomic regions of four soybean chromosomes that contained prospective candidate genes associated with aphid resistance. Two chromosomes have never been reported before. Identification of novel resistance sources allows the possibility of using the accessions and SNPs for marker assisted selection in plant breeding programs, reducing the time needed to develop resistant soybean genotypes.
Our RNA-seq study revealed that pyramiding the two aphid-resistance genes in one soybean genotype resulted in a synergistic effect on resistance to soybean aphids at the molecular level. Specifically, there were sets of differentially expressed genes that were present in the Rag1Rag2 response but absent in the Rag1-alone or Rag2-alone response, indicating activation of different defense pathways in Rag1Rag2 soybean genotype compared to genotypes with individual genes. This project allowed the identification of aphid-responsive genes that can be targeted in future functional genomics studies to validate their role in resistance to soybean aphids.
Subjects/Keywords: Aphids; Breeding; Genes; Plant; Resistance; Soybean; Agriculture; Genetics; Plant Sciences
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APA (6th Edition):
Ibore, M. (2017). Genetic architecture of antibiosis and antixenosis aphid resistance in soybean and elucidation of the molecular effect of pyramiding Rag1 and Rag2 resistance genes. (Thesis). Iowa State University. Retrieved from https://lib.dr.iastate.edu/etd/16517
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Ibore, Martha. “Genetic architecture of antibiosis and antixenosis aphid resistance in soybean and elucidation of the molecular effect of pyramiding Rag1 and Rag2 resistance genes.” 2017. Thesis, Iowa State University. Accessed September 17, 2019.
https://lib.dr.iastate.edu/etd/16517.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Ibore, Martha. “Genetic architecture of antibiosis and antixenosis aphid resistance in soybean and elucidation of the molecular effect of pyramiding Rag1 and Rag2 resistance genes.” 2017. Web. 17 Sep 2019.
Vancouver:
Ibore M. Genetic architecture of antibiosis and antixenosis aphid resistance in soybean and elucidation of the molecular effect of pyramiding Rag1 and Rag2 resistance genes. [Internet] [Thesis]. Iowa State University; 2017. [cited 2019 Sep 17].
Available from: https://lib.dr.iastate.edu/etd/16517.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Ibore M. Genetic architecture of antibiosis and antixenosis aphid resistance in soybean and elucidation of the molecular effect of pyramiding Rag1 and Rag2 resistance genes. [Thesis]. Iowa State University; 2017. Available from: https://lib.dr.iastate.edu/etd/16517
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Iowa State University
27.
De La Fuente, Gerald Neil.
Improvements to the maize (Zea mays L.) in vivo maternal doubled haploid system.
Degree: 2015, Iowa State University
URL: https://lib.dr.iastate.edu/etd/14767
► The maize (Zea mays L.) in vivo maternal doubled haploid system has been widely applied to maize breeding and genetics in recent decades and is…
(more)
▼ The maize (Zea mays L.) in vivo maternal doubled haploid system has been widely applied to maize breeding and genetics in recent decades and is an important part of the majority of public and private maize breeding programs today. The principal advantage of the doubled haploid system is the ability to generate completely homozygous inbred lines in as little as two seasons. Other advantages to this system include more rapid integration of loci of interest and increased usefulness over traditional lines developed through self-pollination. In this dissertation, some of the major problems in the maternal doubled haploid system are addressed. Namely, improvement of maternal inducers, improved understanding of the genetics controlling inducibility, development of an automated system to sort haploid kernels, investigation and application of spontaneous chromosome doubling, and a proposal for the acceleration of the breeding cycle beyond doubled haploids through the in vitro nursery. This dissertation provides some new insight into these problems, as follows. The development and release of a new improved maternal haploid inducer for use in doubled haploid programs. Improved understanding of the quantitative nature of inducibility and the effects of misclassification are discussed. Successful automated discrimination of haploid and diploid kernels using optical and fluorescence methods is described. In an effort to make the doubled haploid system more efficient and safe, a bypass of the colchicine doubling step is proposed through the application and investigation of spontaneous chromosome doubling in haploid plants. Finally, as a proposal for what could be the next step in accelerating the breeding cycle, the in vitro nursery and its applications is discussed.
Subjects/Keywords: Plant Breeding; doubled haploids; maize; Agriculture; Genetics; Plant Sciences
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APA (6th Edition):
De La Fuente, G. N. (2015). Improvements to the maize (Zea mays L.) in vivo maternal doubled haploid system. (Thesis). Iowa State University. Retrieved from https://lib.dr.iastate.edu/etd/14767
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
De La Fuente, Gerald Neil. “Improvements to the maize (Zea mays L.) in vivo maternal doubled haploid system.” 2015. Thesis, Iowa State University. Accessed September 17, 2019.
https://lib.dr.iastate.edu/etd/14767.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
De La Fuente, Gerald Neil. “Improvements to the maize (Zea mays L.) in vivo maternal doubled haploid system.” 2015. Web. 17 Sep 2019.
Vancouver:
De La Fuente GN. Improvements to the maize (Zea mays L.) in vivo maternal doubled haploid system. [Internet] [Thesis]. Iowa State University; 2015. [cited 2019 Sep 17].
Available from: https://lib.dr.iastate.edu/etd/14767.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
De La Fuente GN. Improvements to the maize (Zea mays L.) in vivo maternal doubled haploid system. [Thesis]. Iowa State University; 2015. Available from: https://lib.dr.iastate.edu/etd/14767
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Aberystwyth University
28.
Manwaring, Hanna Rose.
Accessing and dissecting useful genetic variations for iron and zinc content in pearl millet (Pennisetum glaucum) using genome wide association studies.
Degree: PhD, 2018, Aberystwyth University
URL: http://hdl.handle.net/2160/80bc0075-8c54-4e04-ae56-a472882a712f
► Iron/zinc deficiencies cause extensive health problems in developing countries and contribute to a loss of human potential. Many who suffer from micronutrient deficiencies are dependent…
(more)
▼ Iron/zinc deficiencies cause extensive health problems in developing countries and contribute to a loss of human potential. Many who suffer from micronutrient deficiencies are dependent on staple crops to meet their dietary requirements. Thus, the biofortification of crop cultivars with elevated levels of grain micronutrients is becoming increasingly attractive and is largely facilitated by genetics/genomic platforms. Pearl millet is an excellent candidate for biofortification as it is accessible to many populations suffering with micronutrient malnutrition. It contains naturally high levels of micronutrients and thrives in dry, semi-arid regions where farming conditions are often unfavourable; therefore it is considered climate change ready. The aim of this project was to utilise natural genetic variations, present in a germplasm diversity panel of pearl millet to identify genes associated with iron/zinc uptake, with potential to contribute to the development of micronutrient-rich varieties. Iron/zinc levels were quantified in 230 lines by Inductively Coupled Plasma-Atomic Emission Spectroscopy (ICP-AES) and ranged between 29.18-135.27mg/kg and 22.07-93.28mg/kg, respectively. Anti-nutritional factors affecting mineral bioavailability were also considered, including phytate and metal-chelating soluble phenolics. STRUCTURE analysis using Single Nucleotide Polymorphisms (SNPs), generated by Genotyping by Sequencing (GBS) revealed insignificant population structure, further supported by principal component analysis. The extent of Linkage Disequilibrium (LD) was also assessed among all pairs of loci and was found to be prominent on chromosomes 3 and 5. Genome Wide Association Studies (GWAS) resulted in hundreds of significant marker-trait-associations for grain iron/zinc content with p-values ranging from 3.99 E-06-7.54 E-07. Using the 4kb region surrounding the 35 most significant SNPs, a BLAST search of the NCBI database revealed 6 candidate genes associated with iron/zinc uptake. The most significant was YUCCA11, which drives zinc efficiency via auxin biosynthesis. Additionally, haplotypes covering the YUCCA11 gene were identified and their association with trait data was assessed.
Subjects/Keywords: plant genetics; micronutrients; GWAS; genomics; plant breeding; micronutrient malnutrition
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APA (6th Edition):
Manwaring, H. R. (2018). Accessing and dissecting useful genetic variations for iron and zinc content in pearl millet (Pennisetum glaucum) using genome wide association studies. (Doctoral Dissertation). Aberystwyth University. Retrieved from http://hdl.handle.net/2160/80bc0075-8c54-4e04-ae56-a472882a712f
Chicago Manual of Style (16th Edition):
Manwaring, Hanna Rose. “Accessing and dissecting useful genetic variations for iron and zinc content in pearl millet (Pennisetum glaucum) using genome wide association studies.” 2018. Doctoral Dissertation, Aberystwyth University. Accessed September 17, 2019.
http://hdl.handle.net/2160/80bc0075-8c54-4e04-ae56-a472882a712f.
MLA Handbook (7th Edition):
Manwaring, Hanna Rose. “Accessing and dissecting useful genetic variations for iron and zinc content in pearl millet (Pennisetum glaucum) using genome wide association studies.” 2018. Web. 17 Sep 2019.
Vancouver:
Manwaring HR. Accessing and dissecting useful genetic variations for iron and zinc content in pearl millet (Pennisetum glaucum) using genome wide association studies. [Internet] [Doctoral dissertation]. Aberystwyth University; 2018. [cited 2019 Sep 17].
Available from: http://hdl.handle.net/2160/80bc0075-8c54-4e04-ae56-a472882a712f.
Council of Science Editors:
Manwaring HR. Accessing and dissecting useful genetic variations for iron and zinc content in pearl millet (Pennisetum glaucum) using genome wide association studies. [Doctoral Dissertation]. Aberystwyth University; 2018. Available from: http://hdl.handle.net/2160/80bc0075-8c54-4e04-ae56-a472882a712f
University of Kentucky
29.
Fan, Cui.
Evolutionary and functional characterization of Os-POLLUX, a rice gene orthologous to a common symbiosis gene in legume.
Degree: 2008, University of Kentucky
URL: http://uknowledge.uky.edu/gradschool_theses/554
► Root symbioses with arbuscular mycorrhizal fungi and rhizobial bacteria share a common signaling pathway in legumes. Among the common symbiosis genes are CASTOR and POLLUX,…
(more)
▼ Root symbioses with arbuscular mycorrhizal fungi and rhizobial bacteria share a common signaling pathway in legumes. Among the common symbiosis genes are CASTOR and POLLUX, the twin homologous genes in Lotus japonicus that encode putative ion channel proteins. Orthologs of CASTOR and POLLUX are ubiquitously present in both legumes and non-legumes, but their function in non-legumes remains to be elucidated. Here, we use reverse genetic approaches to demonstrate that the rice (Oryza sativa) ortholog of POLLUX, namely Os-POLLUX, is indispensible for mycorrhizal symbiosis in rice. Furthermore, we show that Os-POLLUX can restore nodulation, but not rhizobial infection, to a M. truncatula dmi1 mutant.
Subjects/Keywords: Reverse genetics|rice|root symbiosis|mycorrhization|nodulation; Plant Breeding and Genetics; Plant Sciences
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Manager
APA (6th Edition):
Fan, C. (2008). Evolutionary and functional characterization of Os-POLLUX, a rice gene orthologous to a common symbiosis gene in legume. (Masters Thesis). University of Kentucky. Retrieved from http://uknowledge.uky.edu/gradschool_theses/554
Chicago Manual of Style (16th Edition):
Fan, Cui. “Evolutionary and functional characterization of Os-POLLUX, a rice gene orthologous to a common symbiosis gene in legume.” 2008. Masters Thesis, University of Kentucky. Accessed September 17, 2019.
http://uknowledge.uky.edu/gradschool_theses/554.
MLA Handbook (7th Edition):
Fan, Cui. “Evolutionary and functional characterization of Os-POLLUX, a rice gene orthologous to a common symbiosis gene in legume.” 2008. Web. 17 Sep 2019.
Vancouver:
Fan C. Evolutionary and functional characterization of Os-POLLUX, a rice gene orthologous to a common symbiosis gene in legume. [Internet] [Masters thesis]. University of Kentucky; 2008. [cited 2019 Sep 17].
Available from: http://uknowledge.uky.edu/gradschool_theses/554.
Council of Science Editors:
Fan C. Evolutionary and functional characterization of Os-POLLUX, a rice gene orthologous to a common symbiosis gene in legume. [Masters Thesis]. University of Kentucky; 2008. Available from: http://uknowledge.uky.edu/gradschool_theses/554
Iowa State University
30.
Goiffon, Matthew Daniel.
Optimal population value selection: A population-based selection strategy for genomic selection.
Degree: 2016, Iowa State University
URL: https://lib.dr.iastate.edu/etd/15704
► In order to feed the world’s growing population, an interdisciplinary effort is needed. In this thesis, operations research tools of mathematical modeling, optimization, and simulation…
(more)
▼ In order to feed the world’s growing population, an interdisciplinary effort is needed. In this thesis, operations research tools of mathematical modeling, optimization, and simulation are used to improve an existing plant breeding method, genomic selection. To do this, a new method, called optimal population value (OPV) selection, is proposed. In this paper, OPV selection is first defined as an optimization problem that selects a breeding population using a population metric, instead of individual metrics. Then, OPV selection is thoroughly tested in a simulation study against the existing methods of genomic selection, weighted genomic selection, and optimal haploid value selection. From the results of the simulation study, up to an 8.3%, or 0.58 base standard deviations, greater mean response can be expected than when using traditional genomic selection. These results suggest that population-based selection methods are a promising future research direction.
Subjects/Keywords: Genetics; Genomic selection; Optimization; Plant breeding; Simulation; Systems Engineering; Agriculture; Genetics; Industrial Engineering; Plant Sciences
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Manager
APA (6th Edition):
Goiffon, M. D. (2016). Optimal population value selection: A population-based selection strategy for genomic selection. (Thesis). Iowa State University. Retrieved from https://lib.dr.iastate.edu/etd/15704
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Goiffon, Matthew Daniel. “Optimal population value selection: A population-based selection strategy for genomic selection.” 2016. Thesis, Iowa State University. Accessed September 17, 2019.
https://lib.dr.iastate.edu/etd/15704.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Goiffon, Matthew Daniel. “Optimal population value selection: A population-based selection strategy for genomic selection.” 2016. Web. 17 Sep 2019.
Vancouver:
Goiffon MD. Optimal population value selection: A population-based selection strategy for genomic selection. [Internet] [Thesis]. Iowa State University; 2016. [cited 2019 Sep 17].
Available from: https://lib.dr.iastate.edu/etd/15704.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Goiffon MD. Optimal population value selection: A population-based selection strategy for genomic selection. [Thesis]. Iowa State University; 2016. Available from: https://lib.dr.iastate.edu/etd/15704
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
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