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You searched for subject:(Pirin). Showing records 1 – 2 of 2 total matches.

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University of Illinois – Chicago

1. Orozco-Nunnelly, Danielle A. Determining the Role of Pirin1 in Light-Regulated Growth and Cellular Responses in Arabidopsis thaliana.

Degree: 2015, University of Illinois – Chicago

As photoautotrophic and sessile organisms, plants are both reliant upon, and vulnerable to abiotic signals. Different wavelengths of light provide information for plant development, but these signals can also become stressors at specific levels. PRN1, a G protein α effector and a member of the cupin superfamily, is reported to be involved in the transcription of a light-regulated gene, as well as in seed germination in <i>Arabidopsis thaliana</i>. My work herein reveals that PRN1 can act as a quercetinase <i>in vitro</i> and that this activity is regulated by the activation status of the G protein α subunit. My research also demonstrates that <i>prn1</i> null mutants contain more of the UV-screening quercetin compound, and thus can withstand longer amounts of UV-C radiation than wild type seedlings. This thesis pioneers the use of PRN1 in plant transgenes, where it is demonstrated that both the overexpression of PRN1, and the lack of PRN1 cause disoriented growth in response to white light. Moreover, <i>PRN</i>::PRN1-GFP plants indicate that PRN1 is a mainly nuclear localized protein, with diffuse expression in other cellular areas. While the PRN1 transcript and protein are not strictly diurnally or circadian regulated, Western blot analysis indicates that the protein levels in the cell vary in response to light conditions. When <i>in vitro</i> translated PRN1 protein has been treated briefly with a total cell extract (with only large cell debris removed), there is a shift in band size to higher molecular mass, indicating post-translational modification of PRN1. Treatment of wild type plants with MG-132, a cell permeable proteasome inhibitor, indicates that the larger molecular weight species (corresponding to the anti-PRN1 specific antibody) are likely ubiquitinated. These data reveal many activities of and indicate the regulation of PRN1, and demonstrate the important roles that this protein may play in light-regulated growth and cellular responses to light in <i>Arabidopsis thaliana</i>. Advisors/Committee Members: Okkema, Peter (advisor), Kay, Brian (committee member), Rasenick, Mark (committee member), Stone, David (committee member), Warpeha, Katherine (committee member).

Subjects/Keywords: Arabidopsis; G protein pathway; Light signaling; Photomorphogenesis; Pirin; Plant transgenes; Posttranslational modification; Quercetin; Regulation of transcript and protein; Seedling development

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6th Edition):

Orozco-Nunnelly, D. A. (2015). Determining the Role of Pirin1 in Light-Regulated Growth and Cellular Responses in Arabidopsis thaliana. (Thesis). University of Illinois – Chicago. Retrieved from http://hdl.handle.net/10027/19577

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16th Edition):

Orozco-Nunnelly, Danielle A. “Determining the Role of Pirin1 in Light-Regulated Growth and Cellular Responses in Arabidopsis thaliana.” 2015. Thesis, University of Illinois – Chicago. Accessed January 19, 2020. http://hdl.handle.net/10027/19577.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7th Edition):

Orozco-Nunnelly, Danielle A. “Determining the Role of Pirin1 in Light-Regulated Growth and Cellular Responses in Arabidopsis thaliana.” 2015. Web. 19 Jan 2020.

Vancouver:

Orozco-Nunnelly DA. Determining the Role of Pirin1 in Light-Regulated Growth and Cellular Responses in Arabidopsis thaliana. [Internet] [Thesis]. University of Illinois – Chicago; 2015. [cited 2020 Jan 19]. Available from: http://hdl.handle.net/10027/19577.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Orozco-Nunnelly DA. Determining the Role of Pirin1 in Light-Regulated Growth and Cellular Responses in Arabidopsis thaliana. [Thesis]. University of Illinois – Chicago; 2015. Available from: http://hdl.handle.net/10027/19577

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

2. Turumtay, Halbay. Cell wall engineering for better conversion of lignocellulosic biomass.

Degree: 2014, Ghent University

The objective of our research was to use the flexibility of the cell wall to its extremes by modifying its composition while avoiding deleterious effects on plant physiology. One of the strategies to achieve this is the heterologous expression of genes coding for cell wall degrading (CWD) enzymes in plants. These can alter the cell wall structure during plant growth and might improve saccharification yield of the lignocellulosic biomass. In this PhD study 29 genes coding for cell wall degrading enzymes (66 different constructs) were introduced in Arabidopsis and one of the enzymes (a galactanase) was studied in detail. Interestingly, galactanase expression improved the saccharification efficiency without causing a dramatic yield penalty. In parallel, an attempt was made to modify cell wall properties by the expression of the A. caulinodans NodC gene in Arabidopsis. The obtained transgenic lines accumulated GlcNAc mono- and oligosaccharides in their apoplast, which influenced the overall cell wall architecture and modified the cell wall properties. Furthermore, since lignin is major limiting factor that affect saccharification yield, reducing lignin content became a potential strategy to improve the saccharification yield. Here, we have generated Arabidopsis lines with an altered PIRIN2 and PIRIN4 expression. The lines had an altered lignin composition, reduced lignin content and an improved saccharification yield. Despite their modified cell wall, no dramatic effect on plant growth and development was detected. Advisors/Committee Members: Gheysen, Godelieve, Vanholme, Bartel.

Subjects/Keywords: Biology and Life Sciences; Cell Wall; Bio-fuels; Cell wall degrading enzymes; Galactanase; Lignocellulosic biomass; Pirin; NODC

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6th Edition):

Turumtay, H. (2014). Cell wall engineering for better conversion of lignocellulosic biomass. (Thesis). Ghent University. Retrieved from http://hdl.handle.net/1854/LU-5750077

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16th Edition):

Turumtay, Halbay. “Cell wall engineering for better conversion of lignocellulosic biomass.” 2014. Thesis, Ghent University. Accessed January 19, 2020. http://hdl.handle.net/1854/LU-5750077.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7th Edition):

Turumtay, Halbay. “Cell wall engineering for better conversion of lignocellulosic biomass.” 2014. Web. 19 Jan 2020.

Vancouver:

Turumtay H. Cell wall engineering for better conversion of lignocellulosic biomass. [Internet] [Thesis]. Ghent University; 2014. [cited 2020 Jan 19]. Available from: http://hdl.handle.net/1854/LU-5750077.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Turumtay H. Cell wall engineering for better conversion of lignocellulosic biomass. [Thesis]. Ghent University; 2014. Available from: http://hdl.handle.net/1854/LU-5750077

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

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