subject:(Photosensitizer)

Loyola University Chicago

1. Donahue, Laura Jean. Design, Synthesis, and Biological Evaluation of Folate Targeted Photosensitizers for Photodynamic Therapy.

Degree: PhD, Chemistry, 2015, Loyola University Chicago

URL: https://ecommons.luc.edu/luc_diss/1940

► Photodynamic therapy (PDT) is used to treat cancer, and involves a highly conjugated molecule, called a photosensitizer (PS), which is excited by wavelengths of… (more)

▼ Photodynamic therapy (PDT) is used to treat cancer, and involves a highly conjugated molecule, called a photosensitizer (PS), which is excited by wavelengths of light from visible to infrared. Photosensitizers that are highly conjugated will absorb longer wavelengths (600 – 900 nm), and have the potential to destroy cells of deeper tissue cancers. In PDT, a PS is administered to the patient, and after an appropriate time delay, the tissue is then exposed to light of a specific wavelength necessary for excitation of the PS. A transfer of energy can take place between the excited PS and oxygen that is nearby. This process allows the PS to relax back to the ground state, while creating an excited singlet state oxygen molecule (1O2). Buchwald-Hartwig conditions using Pd catalysts and phosphorus containing ligands can be utilized to synthesize the photosensitizer. The reaction involves a Pd-catalyzed cross coupling of an aryl halide with a nitrogen nucleophile to form a new carbon-nitrogen bond. The PS will be PEGylated with an amine–PEG–Folate complex. Since many tumors over-express folate receptors, the PS–PEG–FA complex will be transported to cancer cells via receptor mediated endocytosis, providing greater selectivity. The killing efficiency of the PS will be tested using HeLa cells. Solutions containing PS only, PS–PEG–FA, and media only will be added to cancer cells with folate restricted media. After exposure to light, the cells will be counted to determine the percentage of cells killed by the singlet oxygen and other radical species. Variables that must be controlled in this study are the following: length of time cells have been growing; specific concentration of PS–PEG–folate complex; length of time for PS–PEG–folate complex uptake into the cell; length of time of exposure to light; length of time from light exposure to cell counting to determine necrosis. The goal of the research is to determine the concentration of PS and length of time of light exposure that is maximally effective for killing tumor cells.

Subjects/Keywords: Chlorin e6 - PEG - FA; Folate Targeted Photosensitizer; Phenothiazine Trimer; Photodynamic Therapy; Photosensitizer; Chemistry

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APA (6^th Edition):

Donahue, L. J. (2015). Design, Synthesis, and Biological Evaluation of Folate Targeted Photosensitizers for Photodynamic Therapy. (Doctoral Dissertation). Loyola University Chicago. Retrieved from https://ecommons.luc.edu/luc_diss/1940

Chicago Manual of Style (16^th Edition):

Donahue, Laura Jean. “Design, Synthesis, and Biological Evaluation of Folate Targeted Photosensitizers for Photodynamic Therapy.” 2015. Doctoral Dissertation, Loyola University Chicago. Accessed January 23, 2021. https://ecommons.luc.edu/luc_diss/1940.

MLA Handbook (7^th Edition):

Donahue, Laura Jean. “Design, Synthesis, and Biological Evaluation of Folate Targeted Photosensitizers for Photodynamic Therapy.” 2015. Web. 23 Jan 2021.

Vancouver:

Donahue LJ. Design, Synthesis, and Biological Evaluation of Folate Targeted Photosensitizers for Photodynamic Therapy. [Internet] [Doctoral dissertation]. Loyola University Chicago; 2015. [cited 2021 Jan 23]. Available from: https://ecommons.luc.edu/luc_diss/1940.

Council of Science Editors:

Donahue LJ. Design, Synthesis, and Biological Evaluation of Folate Targeted Photosensitizers for Photodynamic Therapy. [Doctoral Dissertation]. Loyola University Chicago; 2015. Available from: https://ecommons.luc.edu/luc_diss/1940

2. Bernal, Claudia. Hipericina, Photodithazine e Photogem: um estudo comparativo da atividade fotodinâmica.

Degree: PhD, Química Analítica, 2011, University of São Paulo

URL: http://www.teses.usp.br/teses/disponiveis/75/75132/tde-21062011-094049/ ;

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A Terapia Fotodinâmica (TFP) é uma técnica para tratamento de câncer que usa um fotossensibilizador (FS) na presença de luz e oxigênio gerando espécies altamente… (more)

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A Terapia Fotodinâmica (TFP) é uma técnica para tratamento de câncer que usa um fotossensibilizador (FS) na presença de luz e oxigênio gerando espécies altamente reativas de oxigênio que levam as células tumorais à morte. Neste trabalho foi realizado um estudo comparativo com três FSs: Photogem® (PG), um derivado de hematoporfirina que está sendo usado em TFD no Brasil; Photodithazine® (PZ), um derivado hidrossolúvel de mono-L-aspartil clorina, que está na fase clínica para aprovação e Hipericina (HY), um pigmento fotoativo encontrado na planta Hypericum perforatum e usado na medicina popular que está sendo considerado como um promissor agente fotodinâmico para o tratamento de tumores. Este estudo utilizou uma Hipericina sintetizada no Brasil e diversos parâmetros para comparar os três FSs: a concentração inibitória média (IC50) em linhagens celulares; a constante de velocidade de fotoxidação da albumina de soro bovino na presença dos FSs e luz determinada pelo decréscimo na fluorescência da BSA em 340 nm; a fotoxidação do ácido úrico acompanhada pelo decréscimo da banda característica do ácido úrico em 290 nm após irradiação na presença dos FSs como uma estimativa indireta do rendimento quântico de formação de oxigênio singlete (ΔΦ); o rendimento quântico de fluorescência utilizando rodamina B como padrão; a acumulação dos FSs em células em função do tempo de incubação e a estimativa da quantidade de radicais livres formados após irradiação através da técnica de captura de spins. Todos os resultados obtidos evidenciam uma maior eficiência fotodinâmica da HY seguida pelo PZ e depois por Photogem e, portanto sugerem a Hipericina como o FS de maior potencial para utilização em Terapia Fotodinâmica.

Photodynamic Therapy (PDT) is a technique for the cancer treatment that uses a photosensitizer (FS) in the presence of light and oxygen which combined are able to generate highly reactive oxygen species that lead to tumor cells death. In this investigation, a comparative study with three FSs: Photogem ® (PG), a hematoporphyrin derivative being used in PDT in Brazil; Photodithazine ® (PZ), a soluble derivative of mono-L-aspartyl chlorin, which is in clinical phase for approval and Hypericin (HY), a photoactive pigment found in the plant Hypericum perforatum and used in popular medicine that is being considered as a promising agent for photodynamic treatment of tumors. The present study used a Hypericin synthesized in Brazil and several parameters to compare these three FSs: the mean inhibitory concentration (IC50) in cell lines; the rate constant for the photooxidation of bovine serum albumin in the presence of light and the FSs determined by the decrease in the fluorescence of BSA at 340 nm; the photooxidation of uric acid assessed by the decrease of the characteristic band of uric acid at 290 nm after irradiation in the presence of the FSs as an indirect estimate of the quantum yield of formation of singlet oxygen (ΔΦ); the quantum yield of fluorescence using rhodamine B as…

Advisors/Committee Members: Perussi, Janice Rodrigues.

Subjects/Keywords: Cell culture; Cultura celular; Fotossensibilizador; Photodynamic therapy; Photosensitizer; Terapia fotodinâmica

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APA (6^th Edition):

Bernal, C. (2011). Hipericina, Photodithazine e Photogem: um estudo comparativo da atividade fotodinâmica. (Doctoral Dissertation). University of São Paulo. Retrieved from http://www.teses.usp.br/teses/disponiveis/75/75132/tde-21062011-094049/ ;

Chicago Manual of Style (16^th Edition):

Bernal, Claudia. “Hipericina, Photodithazine e Photogem: um estudo comparativo da atividade fotodinâmica.” 2011. Doctoral Dissertation, University of São Paulo. Accessed January 23, 2021. http://www.teses.usp.br/teses/disponiveis/75/75132/tde-21062011-094049/ ;.

MLA Handbook (7^th Edition):

Bernal, Claudia. “Hipericina, Photodithazine e Photogem: um estudo comparativo da atividade fotodinâmica.” 2011. Web. 23 Jan 2021.

Vancouver:

Bernal C. Hipericina, Photodithazine e Photogem: um estudo comparativo da atividade fotodinâmica. [Internet] [Doctoral dissertation]. University of São Paulo; 2011. [cited 2021 Jan 23]. Available from: http://www.teses.usp.br/teses/disponiveis/75/75132/tde-21062011-094049/ ;.

Council of Science Editors:

Bernal C. Hipericina, Photodithazine e Photogem: um estudo comparativo da atividade fotodinâmica. [Doctoral Dissertation]. University of São Paulo; 2011. Available from: http://www.teses.usp.br/teses/disponiveis/75/75132/tde-21062011-094049/ ;

3. Gonçalves, Joyce Laura da Silva. Caracterização físico-química e fotodinâmica de fotossensibilizadores: efeito da modificação química para aumentar a solubilidade em meio aquoso.

Degree: PhD, Química Analítica e Inorgânica, 2015, University of São Paulo

URL: http://www.teses.usp.br/teses/disponiveis/75/75135/tde-29062015-153459/ ;

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A hidrofobicidade e a estrutura planar do orbital π estendido de fotossensibilizadores do tipo clorina e hipericina podem favorecer a agregação destes compostos em meio… (more)

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A hidrofobicidade e a estrutura planar do orbital π estendido de fotossensibilizadores do tipo clorina e hipericina podem favorecer a agregação destes compostos em meio aquoso. Esta agregação pode reduzir a eficiência fotodinâmica e a aplicabilidade destes compostos em diagnósticos e na Terapia fotodinâmica. Uma estratégia para minimizar esta limitação é a modificação destas moléculas pela inserção de grupos hidrofílicos. Neste trabalho foram utilizadas técnicas espectroscópicas para caracterizar as propriedades físico-químicas e fotodinâmicas de derivados de clorina (CHL), e hipericina (HY) obtidos por meio de inserções dos grupos hidrofílicos trizma e glucamina, respectivamente: Clorina-Trizma (CHL-T) e Hipericina Glucamina (HY-G). Os resultados mostraram que estas modificações estruturais aumentaram em até 20% a solubilidade destes compostos em meio aquoso. No entanto, devido à solubilidade parcial dos fotossensibilizadores na ausência de cargas elétricas foram identificados agregados do tipo H em meio ácido, neutro e na presença de íons em solução aquosa. Tais agregados foram solubilizados em meio alcalino e por microambientes micelares dos surfactantes CTAB, SDS e Tween 20. Os agregados do tipo H acarretaram ainda na redução da constante de velocidade de fotobranqueamento e da formação de oxigênio singleto dos fotossensibilizadores em meio aquoso. Contudo, as clorinas foram cerca de 15 vezes mais eficientes do que a hipericinas na geração deste radical citotóxico. A análise sistemática do potencial fotodinâmico dos fotossensibilizadores em células VERO e HUVEC (não tumorais) e HEp-2 (tumoral) foi realizada por meio de um planejamento fatorial combinando-se a concentração, tempo de acumulação do fotossensibilizador no interior da célula e a dose de luz. Esta análise mostrou que o tempo de acumulação do fotossensibilizador é um parâmetro significante para se erradicar seletivamente as células cancerígenas. Ao contrário das células não tumorais, nas células HEp-2 a quantidade de fotossensibilizador acumulado foi proporcional à lipoficilidade dos fotossensibilizadores. A análise quimiométrica resultou ainda em um modelo matemático para a estimativa dos valores da concentração inibitória média que foi validada por meio de comparação estatística com os valores experimentais determinados para os fotossensibilizadores. As hipericinas foram mais fototóxicas para as células tumorais do que as clorinas. Nas células não tumorais os derivados foram menos citotóxicos, sugerindo o uso destes compostos para a inativação seletiva de células tumorais. Todas essas características permitem que os compostos sejam empregados como fotossensibilizadores em diagnósticos e tratamentos fotodinâmicos.

The hydrophobicity and planar structure of π extended orbital of photosensitizers like chlorine and hypericin may favor the aggregation of these compounds in aqueous medium. This aggregation can reduce their photodynamic efficiency and applicability in Photodynamic Therapy and diagnosis. A strategy to minimize this limitation is the…

Advisors/Committee Members: Imasato, Hidetake, Perussi, Janice Rodrigues.

Subjects/Keywords: fotossensibilizador; hidrossolubilidade e planejamento fatorial; photosensitizer; water solubility and factorial design

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Gonçalves, J. L. d. S. (2015). Caracterização físico-química e fotodinâmica de fotossensibilizadores: efeito da modificação química para aumentar a solubilidade em meio aquoso. (Doctoral Dissertation). University of São Paulo. Retrieved from http://www.teses.usp.br/teses/disponiveis/75/75135/tde-29062015-153459/ ;

Chicago Manual of Style (16^th Edition):

Gonçalves, Joyce Laura da Silva. “Caracterização físico-química e fotodinâmica de fotossensibilizadores: efeito da modificação química para aumentar a solubilidade em meio aquoso.” 2015. Doctoral Dissertation, University of São Paulo. Accessed January 23, 2021. http://www.teses.usp.br/teses/disponiveis/75/75135/tde-29062015-153459/ ;.

MLA Handbook (7^th Edition):

Gonçalves, Joyce Laura da Silva. “Caracterização físico-química e fotodinâmica de fotossensibilizadores: efeito da modificação química para aumentar a solubilidade em meio aquoso.” 2015. Web. 23 Jan 2021.

Vancouver:

Gonçalves JLdS. Caracterização físico-química e fotodinâmica de fotossensibilizadores: efeito da modificação química para aumentar a solubilidade em meio aquoso. [Internet] [Doctoral dissertation]. University of São Paulo; 2015. [cited 2021 Jan 23]. Available from: http://www.teses.usp.br/teses/disponiveis/75/75135/tde-29062015-153459/ ;.

Council of Science Editors:

Gonçalves JLdS. Caracterização físico-química e fotodinâmica de fotossensibilizadores: efeito da modificação química para aumentar a solubilidade em meio aquoso. [Doctoral Dissertation]. University of São Paulo; 2015. Available from: http://www.teses.usp.br/teses/disponiveis/75/75135/tde-29062015-153459/ ;

Vanderbilt University

4. Mitchell, Courtney Alexis. Understanding the mechanisms of blue light irradiation-induced growth reduction of pathogenic E. coli.

Degree: PhD, Chemical Engineering, 2016, Vanderbilt University

URL: http://hdl.handle.net/1803/13818

► Visible light therapy (400-700 nm), i.e. photodynamic therapy, phototherapy, etc. has been used experimentally and clinically for treatment in acne, cancer, wounds, jaundiced neonates and… (more)

▼ Visible light therapy (400-700 nm), i.e. photodynamic therapy, phototherapy, etc. has been used experimentally and clinically for treatment in acne, cancer, wounds, jaundiced neonates and other ailments. In the wake of increasing antibiotic resistance, the application of blue light (400-500 nm) as an antimicrobial strategy is appealing. Previous studies have elucidated differences on the responses of bacteria to irradiation with blue light (or blue light irradiation-BLI), ranging from a decrease in growth for some species, to stimulating proliferation in others. Although effective against a range of Gram-positive pathogens, BLI appears to be less effective at targeting Gram-negative bacteria and the basis of this phenomenon remains unknown. Studies evaluating the phototoxic effect of BLI on bacteria revealed that endogenous photosensitizers absorbing blue light lead to the formation of reactive oxygen species (ROS). In turn, these ROS, specifically singlet oxygen, can have a cytotoxic effect. Conversely, significant literature documents the ability of bacteria to sense and respond to blue light, via the use of BLUF (sensor of blue light using FAD-flavin adenine dinucleotide) domain-containing proteins. Much of the work that has been done has focused on bacteria early in its life cycle, but it is bacteria in later stages of growth that are responsible for causing infections and diseases. Through this work the growth phase dependencies on reductions in Gram-negative Escherichia coli were defined. The reduction differences between non-pathogenic and pathogenic E. coli strains were determined. The endogenous photosensitizer that is involved in the BLI-induced response at the 455 nm wavelength was also identified. Light parameters such as wavelength, energy dose, and the energy flux affect reductions in growth; findings from modulating these parameters were used to further enhance reductions in E. coli post-BLI. Through these studies and additional kinetic analysis, a proposed a model has been developed to describe the observed BLI reductions. Advisors/Committee Members: Maria Hadjifrangiskou (committee member), Scott Guelcher (committee member), Matthew Lang (committee member), Bridget Rogers (Committee Chair).

Subjects/Keywords: Escherichia coli; phototherapy; blue light; photodynamic therapy; photosensitizer; FAD

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Mitchell, C. A. (2016). Understanding the mechanisms of blue light irradiation-induced growth reduction of pathogenic E. coli. (Doctoral Dissertation). Vanderbilt University. Retrieved from http://hdl.handle.net/1803/13818

Chicago Manual of Style (16^th Edition):

Mitchell, Courtney Alexis. “Understanding the mechanisms of blue light irradiation-induced growth reduction of pathogenic E. coli.” 2016. Doctoral Dissertation, Vanderbilt University. Accessed January 23, 2021. http://hdl.handle.net/1803/13818.

MLA Handbook (7^th Edition):

Mitchell, Courtney Alexis. “Understanding the mechanisms of blue light irradiation-induced growth reduction of pathogenic E. coli.” 2016. Web. 23 Jan 2021.

Vancouver:

Mitchell CA. Understanding the mechanisms of blue light irradiation-induced growth reduction of pathogenic E. coli. [Internet] [Doctoral dissertation]. Vanderbilt University; 2016. [cited 2021 Jan 23]. Available from: http://hdl.handle.net/1803/13818.

Council of Science Editors:

Mitchell CA. Understanding the mechanisms of blue light irradiation-induced growth reduction of pathogenic E. coli. [Doctoral Dissertation]. Vanderbilt University; 2016. Available from: http://hdl.handle.net/1803/13818

University of Arizona

5. Barnett, Benjamin Joseph. Modelling and Mechanisms for the Production of Singlet Oxygen in Ideal Systems and Wastewater Effluent .

Degree: 2020, University of Arizona

URL: http://hdl.handle.net/10150/641671

► Trace organic compounds (TOrCs) in natural waters and wastewater effluents are concerning to both environmental health and water reuse. Although some TOrCs are known to… (more)

Subjects/Keywords: Methylene Blue; Photosensitizer; Quantum Yield; Singlet Oxygen; Wastewater

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Barnett, B. J. (2020). Modelling and Mechanisms for the Production of Singlet Oxygen in Ideal Systems and Wastewater Effluent . (Masters Thesis). University of Arizona. Retrieved from http://hdl.handle.net/10150/641671

Chicago Manual of Style (16^th Edition):

Barnett, Benjamin Joseph. “Modelling and Mechanisms for the Production of Singlet Oxygen in Ideal Systems and Wastewater Effluent .” 2020. Masters Thesis, University of Arizona. Accessed January 23, 2021. http://hdl.handle.net/10150/641671.

MLA Handbook (7^th Edition):

Barnett, Benjamin Joseph. “Modelling and Mechanisms for the Production of Singlet Oxygen in Ideal Systems and Wastewater Effluent .” 2020. Web. 23 Jan 2021.

Vancouver:

Barnett BJ. Modelling and Mechanisms for the Production of Singlet Oxygen in Ideal Systems and Wastewater Effluent . [Internet] [Masters thesis]. University of Arizona; 2020. [cited 2021 Jan 23]. Available from: http://hdl.handle.net/10150/641671.

Council of Science Editors:

Barnett BJ. Modelling and Mechanisms for the Production of Singlet Oxygen in Ideal Systems and Wastewater Effluent . [Masters Thesis]. University of Arizona; 2020. Available from: http://hdl.handle.net/10150/641671

University of Toronto

6. Walsh, Connor Dennis. Porphyrin Molecular Beacons – a New Approach to Osteoarthritis.

Degree: 2019, University of Toronto

URL: http://hdl.handle.net/1807/98415

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Despite the substantial burden imposed by osteoarthritis (OA) upon patients and healthcare systems across the world, there are still no effective disease-modifying treatments. A recent… (more)

Subjects/Keywords: fluorescence; matrix metalloproteinase; osteoarthritis; photodynamic therapy; photosensitizer; porphyrin; 0541

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Walsh, C. D. (2019). Porphyrin Molecular Beacons – a New Approach to Osteoarthritis. (Masters Thesis). University of Toronto. Retrieved from http://hdl.handle.net/1807/98415

Chicago Manual of Style (16^th Edition):

Walsh, Connor Dennis. “Porphyrin Molecular Beacons – a New Approach to Osteoarthritis.” 2019. Masters Thesis, University of Toronto. Accessed January 23, 2021. http://hdl.handle.net/1807/98415.

MLA Handbook (7^th Edition):

Walsh, Connor Dennis. “Porphyrin Molecular Beacons – a New Approach to Osteoarthritis.” 2019. Web. 23 Jan 2021.

Vancouver:

Walsh CD. Porphyrin Molecular Beacons – a New Approach to Osteoarthritis. [Internet] [Masters thesis]. University of Toronto; 2019. [cited 2021 Jan 23]. Available from: http://hdl.handle.net/1807/98415.

Council of Science Editors:

Walsh CD. Porphyrin Molecular Beacons – a New Approach to Osteoarthritis. [Masters Thesis]. University of Toronto; 2019. Available from: http://hdl.handle.net/1807/98415

East Tennessee State University

7. Rasheed, Senan. Photocatalytic Carbon Dioxide Reduction with Zinc(II) Dipyrrin Photosensitizers and Iron Catalyst.

Degree: MS, Chemistry, 2020, East Tennessee State University

URL: https://dc.etsu.edu/etd/3730

► Much of the energy used in the United States and around the globe is obtained from petroleum, natural gas, and coal. Photocatalytic CO₂reduction… (more)

Subjects/Keywords: Photocatalysis; CO2; reduction; Photosensitizer; Zinc; Iron catalyst; Chemistry

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APA (6^th Edition):

Rasheed, S. (2020). Photocatalytic Carbon Dioxide Reduction with Zinc(II) Dipyrrin Photosensitizers and Iron Catalyst. (Thesis). East Tennessee State University. Retrieved from https://dc.etsu.edu/etd/3730

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Rasheed, Senan. “Photocatalytic Carbon Dioxide Reduction with Zinc(II) Dipyrrin Photosensitizers and Iron Catalyst.” 2020. Thesis, East Tennessee State University. Accessed January 23, 2021. https://dc.etsu.edu/etd/3730.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Rasheed, Senan. “Photocatalytic Carbon Dioxide Reduction with Zinc(II) Dipyrrin Photosensitizers and Iron Catalyst.” 2020. Web. 23 Jan 2021.

Vancouver:

Rasheed S. Photocatalytic Carbon Dioxide Reduction with Zinc(II) Dipyrrin Photosensitizers and Iron Catalyst. [Internet] [Thesis]. East Tennessee State University; 2020. [cited 2021 Jan 23]. Available from: https://dc.etsu.edu/etd/3730.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Rasheed S. Photocatalytic Carbon Dioxide Reduction with Zinc(II) Dipyrrin Photosensitizers and Iron Catalyst. [Thesis]. East Tennessee State University; 2020. Available from: https://dc.etsu.edu/etd/3730

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Washington University in St. Louis

8. Lu, Chen. Effect of DNA Sequence Context, DNA structure, and Excitation Method on Cyclobutane Pyrimidine Dimer Formation.

Degree: PhD, Chemistry, 2018, Washington University in St. Louis

URL: https://openscholarship.wustl.edu/art_sci_etds/1559

► The cis-syn cyclobutane pyrimdine dimer (CPD) is the major photoproduct resulting from UV irradiation of duplex DNA that results in C to T mutations… (more)

▼ The cis-syn cyclobutane pyrimdine dimer (CPD) is the major photoproduct resulting from UV irradiation of duplex DNA that results in C to T mutations found in human skin. CPDs with the anti stereochemistry were recently discovered to be formed in human telomeric DNA that adopts a quadruplex structure in vitro and may also play a role in the effects of sunlight in vivo. In this thesis, the effect of telomeric DNA structure on the formation of the anti-CPDs is investigated, as well as the effect of sequence context on cis-syn CPD formation which could help explain the origin of DNA mutation hot spots and cold spots. Originally it was hypothesized that anti CPDs formed from G-quadruplex structures, but I now show that anti CPDs can also form in human telomeric DNA sequences when complexed with lithium ions that are known to disfavor G-quadruplex formation. I also show that anti CPDs can also form in the presence of potassium ion when selected guanine bases are changed to inosine to interfere with G-quartet formation. Most significantly weI show that anti-CPDs form in sequences containing A’s in place of G’s that cannot form Hoogsteen hairpins, but can form reverse Hoogsteen hairpins. These results suggest that reverse Hoogsteen hairpins may play a hitherto unrecognized role in the biology and photoreactivity of DNA in telomeres, and possibly in other purine-rich sequences found in regulatory regions. To study sequence context effects, we designed 129-mer sequences containing all 64 possible NPyPyN tetrads (where PyPy is CC, CT, TC, or TT, and N is A, C, G or T), and used a T4 endonuclease gel electrophoresis assay to determine the relative yields of photoproduct formation at the different sites. The results show that CPD yields for different tetrads varied by as much as an order of magnitude. The yield of CPDs under UVC irradiation at a given site decrease in the order TT > TC > CT > CC, whereas the yield of CPDs under UVB irradiation decreased in the order TT > TC > CC > CT. The yield of CPD formation was lowest with a 5’-G and highest with a 5’-T, whereas the yield was lowest with either a 3’-C, G or T, and highest with a 3’-flanking A. We also studied the sequence context effect on photosensitized CPD formation in the presence of acetone and norfloxacin. The results not only show that the efficiency of photosensitized CPD formation depends on the flanking bases, but also indicates that the efficiency depends on the structure and properties of the photosensitizer. These results suggest that the photosensitizers may result in unique mutation spectra that can be used to identify endogenous photosensitizers such as those implicated in chemi-excitation pathways in melanoma induction. Advisors/Committee Members: John-Stephen A. Taylor, Mikhail Berezin, Robert Blankenship, Michael L. Gross, Timothy Wencewicz.

Subjects/Keywords: CPD, DNA photochemistry, photosensitizer, reverse hoogsteen hairpin, sequence context effect; Biochemistry

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Lu, C. (2018). Effect of DNA Sequence Context, DNA structure, and Excitation Method on Cyclobutane Pyrimidine Dimer Formation. (Doctoral Dissertation). Washington University in St. Louis. Retrieved from https://openscholarship.wustl.edu/art_sci_etds/1559

Chicago Manual of Style (16^th Edition):

Lu, Chen. “Effect of DNA Sequence Context, DNA structure, and Excitation Method on Cyclobutane Pyrimidine Dimer Formation.” 2018. Doctoral Dissertation, Washington University in St. Louis. Accessed January 23, 2021. https://openscholarship.wustl.edu/art_sci_etds/1559.

MLA Handbook (7^th Edition):

Lu, Chen. “Effect of DNA Sequence Context, DNA structure, and Excitation Method on Cyclobutane Pyrimidine Dimer Formation.” 2018. Web. 23 Jan 2021.

Vancouver:

Lu C. Effect of DNA Sequence Context, DNA structure, and Excitation Method on Cyclobutane Pyrimidine Dimer Formation. [Internet] [Doctoral dissertation]. Washington University in St. Louis; 2018. [cited 2021 Jan 23]. Available from: https://openscholarship.wustl.edu/art_sci_etds/1559.

Council of Science Editors:

Lu C. Effect of DNA Sequence Context, DNA structure, and Excitation Method on Cyclobutane Pyrimidine Dimer Formation. [Doctoral Dissertation]. Washington University in St. Louis; 2018. Available from: https://openscholarship.wustl.edu/art_sci_etds/1559

9. Mauriello Jimenez, Chiara. Nanoparticules à visées théranostiques pour le traitement du cancer par thérapie photodynamique à un ou deux photons : Theranostic Nanoparticles for cancer treatment using one or two-photon photodynamic therapy.

Degree: Docteur es, Chimie et Physico-Chimie des Matériaux, 2016, Montpellier

URL: http://www.theses.fr/2016MONTT100

►

L'augmentation du nombre de cancers de faible taille dans le monde a incité le développement de nouveaux nanomatériaux multifonctionnels appliqués à de nouvelles thérapies non… (more)

▼

L'augmentation du nombre de cancers de faible taille dans le monde a incité le développement de nouveaux nanomatériaux multifonctionnels appliqués à de nouvelles thérapies non invasives. Ces nouvelles thérapies peuvent éliminer sélectivement la tumeur en réduisant ou en supprimant les effets secondaires induits dans les tissus sains par les traitements actuels, tels que la chimiothérapie ou la radiothérapie, qui présentent une efficacité élevée mais une faible sélectivité. Ce travail décrit l'élaboration de nanomatériaux pour le diagnostic et le traitement des cancers de faible taille grâce à une nouvelle thérapie: la thérapie photodynamique (PDT). Cette nouvelle technique, implique l'activation d'une molécule photosensibilisatrice (PS) grâce à des longueurs d'onde spécifiques. Cette activation conduit à des cascades de transfert d'énergie qui produisent des espèces oxygénées réactives cytotoxiques provoquant la mort cellulaire.Dans un premier temps, l'élaboration de nanoparticules de silice mésoporeuses (MSN) contenant un agent photosensibilisant de type porphyrine est présentée pour le traitement in vitro du cancer de la prostate et du rétinoblastome grâce à la thérapie photodynamique à un photon. Des nanoparticules fonctionnalisées avec de nouveaux ligands ont été essayées pour cibler les nanoparticules vers les cellules cancéreuses de la prostate. La diminution de la taille des nanoparticules à 20 nm a été élaborée pour traverser la barrière hémato-rétinienne et traiter les rétinoblastomes.D'autre part, deux nouveaux types de nanomatériaux ont été conçus pour le traitement à deux photons qui conduit à une pénétration plus profonde dans les tissus. Des nanoparticules polysilsesquioxane pontés (BS) et des nanoparticules d’organosilice mésoporeuses (PMO) ont été conçues à partir de différents types de molécules photosensibilisatrices tétra-silylées ou octa-silylées et de bis-organo-alcoxysilanes comme l'éthane, l'éthylène ou le disulfide. L’efficacité des BS en imagerie à deux photons en thérapie photodynamique a été démontrée in vitro. Des nanoparticules de BS à base de disulfides ont été conçues comme nouveaux nanomatériaux biodégradables.Enfin, en plus de l'imagerie et la thérapie, les PMO ont été testés in vitro pour la délivrance de médicaments en raison de leur mésoporosité. La gemcitabine et doxorubicine ont été encapsulées dans les pores obtenant des charges élevées en médicaments. Outre les photosensibilisateurs classiques, des PMO cœur-coquille contenant des nanodiamants ont été testés en tant que PS. Pour finir, des PMO à base de porphyrines sont présentés pour la délivrance de gènes in vitro et in vivo utilisant le poisson-zèbre comme modèle.

Nowadays, the increase of the number of low-size cancers in the world has prompted the development of novel multifunctional nanomaterials applied to new non-invasive therapies. These new therapies are expected to selectively eradicate the tumor, decreasing or suppressing the side effects induced in healthy tissues by current treatments. This study describes the…

Advisors/Committee Members: Raehm, Laurence (thesis director), Durand, Jean-Olivier (thesis director).

Subjects/Keywords: Thérapie photodynamique; Nanoparticules; Photosensibilisateur; Photodynamic therapy; Nanoparticles; Photosensitizer

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APA (6^th Edition):

Mauriello Jimenez, C. (2016). Nanoparticules à visées théranostiques pour le traitement du cancer par thérapie photodynamique à un ou deux photons : Theranostic Nanoparticles for cancer treatment using one or two-photon photodynamic therapy. (Doctoral Dissertation). Montpellier. Retrieved from http://www.theses.fr/2016MONTT100

Chicago Manual of Style (16^th Edition):

Mauriello Jimenez, Chiara. “Nanoparticules à visées théranostiques pour le traitement du cancer par thérapie photodynamique à un ou deux photons : Theranostic Nanoparticles for cancer treatment using one or two-photon photodynamic therapy.” 2016. Doctoral Dissertation, Montpellier. Accessed January 23, 2021. http://www.theses.fr/2016MONTT100.

MLA Handbook (7^th Edition):

Mauriello Jimenez, Chiara. “Nanoparticules à visées théranostiques pour le traitement du cancer par thérapie photodynamique à un ou deux photons : Theranostic Nanoparticles for cancer treatment using one or two-photon photodynamic therapy.” 2016. Web. 23 Jan 2021.

Vancouver:

Mauriello Jimenez C. Nanoparticules à visées théranostiques pour le traitement du cancer par thérapie photodynamique à un ou deux photons : Theranostic Nanoparticles for cancer treatment using one or two-photon photodynamic therapy. [Internet] [Doctoral dissertation]. Montpellier; 2016. [cited 2021 Jan 23]. Available from: http://www.theses.fr/2016MONTT100.

Council of Science Editors:

Mauriello Jimenez C. Nanoparticules à visées théranostiques pour le traitement du cancer par thérapie photodynamique à un ou deux photons : Theranostic Nanoparticles for cancer treatment using one or two-photon photodynamic therapy. [Doctoral Dissertation]. Montpellier; 2016. Available from: http://www.theses.fr/2016MONTT100

University of Minnesota

10. Boe, Benjamin. Design, Synthesis, And Characterization Of Aluminum(Iii) Porphyrin Assemblies For Use In Photochemical Cells.

Degree: MS, Chemistry, 2020, University of Minnesota

URL: http://hdl.handle.net/11299/215047

► A series of axially-coordinated aluminum(III) porphyrins were synthesized and evaluated as potential photosensitizers of a ruthenium-based water oxidation catalyst. The porphyrins themselves are 5,10,15,20-tetraphenylporphyrins, and… (more)

▼ A series of axially-coordinated aluminum(III) porphyrins were synthesized and evaluated as potential photosensitizers of a ruthenium-based water oxidation catalyst. The porphyrins themselves are 5,10,15,20-tetraphenylporphyrins, and differ in the degree of fluorination on the peripheral phenyl groups. These aluminum(III) porphyrins readily assemble into catalytic dyads through formation of a covalent ester linkage between the central aluminum atom of the porphyrin and a terminal carboxyl group on the ruthenium catalyst. The aluminum center is also able to act as a Lewis acid, forming the final triad by way of a coordinate bond with a suitable Lewis base. Catalytic dyads were successfully synthesized from all three porphyrins in the series; a set of control compounds were also prepared. The dyads and reference molecules were then characterized, with molecular structure and successful formation of the dyads being confirmed with proton NMR spectroscopy, optical properties assessed with respect to UV-Vis absorption and fluorescence spectroscopy, and redox potentials being assessed by both cyclic and differential pulse voltammetry. Formation of the final triad was achieved by titration of the catalytic dyads with a C60 fullerene functionalized to act as a Lewis base; absorption and fluorescence spectra were monitored during titration, allowing for confirmation of the triad formation, as well as calculation of binding constants. The characterization data were used to construct energy level diagrams, laying the groundwork for a theoretical abstraction of these molecule’s functioning. the catalytic systems as synthesized, as well as how they might function in a prototypical photochemical cell. ii Analysis of the results reveal these materials to be promising candidates as photoactivated water oxidation catalysts. The absorption and electrochemical data demonstrate that, when the catalytic dyads are formed, the electronic structure of the constituent parts is preserved. The fluorescence spectra of the dyads show significant quenching relative to the reference porphyrins. Control studies allowed for the exclusion of intermolecular processes as being the source of this quenching, and therefore the optically excited porphyrin must be able to interact with the attached ruthenium catalyst, either through energy or electron transfer. Based on the negligible overlap of the spectra of the catalyst with that of the porphyrins, energy transfer is unlikely. The most likely source of the fluorescence quenching is therefore electron transfer across the ester-bond. The formation and persistence of such a radical ion pair is a fundamental prerequisite for the material to function as a water oxidation catalyst, as it is on this charge-separated species that water oxidation proceeds. Coordination of the dyads with a fullerene ligand was similarly demonstrated, with the resulting triad exhibiting complete fluorescence quenching. The fullerene ligand itself was chosen specifically for its suitability as an electron acceptor, and…

Subjects/Keywords: Green Energy; Materials Chemistry; Photochemical Cell; Photosensitizer; Porphyrin; Water Oxidation

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APA (6^th Edition):

Boe, B. (2020). Design, Synthesis, And Characterization Of Aluminum(Iii) Porphyrin Assemblies For Use In Photochemical Cells. (Masters Thesis). University of Minnesota. Retrieved from http://hdl.handle.net/11299/215047

Chicago Manual of Style (16^th Edition):

Boe, Benjamin. “Design, Synthesis, And Characterization Of Aluminum(Iii) Porphyrin Assemblies For Use In Photochemical Cells.” 2020. Masters Thesis, University of Minnesota. Accessed January 23, 2021. http://hdl.handle.net/11299/215047.

MLA Handbook (7^th Edition):

Boe, Benjamin. “Design, Synthesis, And Characterization Of Aluminum(Iii) Porphyrin Assemblies For Use In Photochemical Cells.” 2020. Web. 23 Jan 2021.

Vancouver:

Boe B. Design, Synthesis, And Characterization Of Aluminum(Iii) Porphyrin Assemblies For Use In Photochemical Cells. [Internet] [Masters thesis]. University of Minnesota; 2020. [cited 2021 Jan 23]. Available from: http://hdl.handle.net/11299/215047.

Council of Science Editors:

Boe B. Design, Synthesis, And Characterization Of Aluminum(Iii) Porphyrin Assemblies For Use In Photochemical Cells. [Masters Thesis]. University of Minnesota; 2020. Available from: http://hdl.handle.net/11299/215047

University of Cincinnati

11. Wang, Peng. Development of Nanoparticle-based Platforms for Potential Applications in Biosensing and Therapeutics.

Degree: PhD, Arts and Sciences: Chemistry, 2017, University of Cincinnati

URL: http://rave.ohiolink.edu/etdc/view?acc_num=ucin151186771296011

► In this dissertation, I first developed and optimize an upconversion nanoparticles-based DNA detection scheme on different target DNA sequences, and then I explored the syntheses… (more)

▼ In this dissertation, I first developed and optimize an upconversion nanoparticles-based DNA detection scheme on different target DNA sequences, and then I explored the syntheses and characterizations of a nanomaterial-photosensitizer platform used for photodynamic therapy of cancer cells and bacteria in vitro. In the first project, a novel ligase-assisted signal-amplifiable DNA detection scheme is demonstrated based on luminescent resonance energy transfer between upconversion nanoparticles and the intercalating dye, SYBR Green I. Target DNA serves as a template for two DNA probes, one of them covalently attached to upconversion nanoparticles, to be joined into a long, hairpin-forming DNA by ligase. The number of the resulting DNA strand, which brings SYBR Green I close to the upconversion nanoparticles, is amplified through thermal cycling. The method was proven to display high sensitivity and specificity for DNA detection. Factors affecting the detection specificity and sensitivity, including ligation temperature, the amount of ligase, and the number of thermal cycles, have been investigated to optimize the performance of the detection method. Based on our result, the detection scheme can easily differentiate the BRAF V600E mutation from the wild-type sequence with a mutant-to-wild-type ratio of 1:1000. A detection limit of 1 fmole BRAF V600E mutation is achieved. While for the target sequence of EGFR T790M, the differentiate ratio is 1:100. The results show that 0.01 pmole of EGFR T790M mutant can be readily detected.In the second project, I report a hybrid singlet oxygen production system, where strong resonance coupling between silver nanoparticles and photosensitizing molecules results in exceptionally high singlet oxygen production under both visible light and near-infrared light excitation, even for the photosensitizing molecules without near-infrared absorption. Also, our results indicate that the hybrid photosensitizers display low cytotoxicity without light illumination yet highly enhanced photodynamic inhibition efficacy against Hela cells under a broad spectrum of light illuminations including the near-infrared light, which has great implication in photodynamic therapy of deep-tissue cancers. In addition, formulation of hybrid photosensitizers dispersed in PEG matrix demonstrates high bacterial killing efficacy against both S. epidermidis and P. acnes, suggesting it has immense potential for treatment of acne vulgaris without involving antibiotics. Advisors/Committee Members: Zhang, Peng (Committee Chair).

Subjects/Keywords: Nanotechnology; upconversion nanoparticle; DNA detection; photodynamic therapy; hybrid photosensitizer

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APA (6^th Edition):

Wang, P. (2017). Development of Nanoparticle-based Platforms for Potential Applications in Biosensing and Therapeutics. (Doctoral Dissertation). University of Cincinnati. Retrieved from http://rave.ohiolink.edu/etdc/view?acc_num=ucin151186771296011

Chicago Manual of Style (16^th Edition):

Wang, Peng. “Development of Nanoparticle-based Platforms for Potential Applications in Biosensing and Therapeutics.” 2017. Doctoral Dissertation, University of Cincinnati. Accessed January 23, 2021. http://rave.ohiolink.edu/etdc/view?acc_num=ucin151186771296011.

MLA Handbook (7^th Edition):

Wang, Peng. “Development of Nanoparticle-based Platforms for Potential Applications in Biosensing and Therapeutics.” 2017. Web. 23 Jan 2021.

Vancouver:

Wang P. Development of Nanoparticle-based Platforms for Potential Applications in Biosensing and Therapeutics. [Internet] [Doctoral dissertation]. University of Cincinnati; 2017. [cited 2021 Jan 23]. Available from: http://rave.ohiolink.edu/etdc/view?acc_num=ucin151186771296011.

Council of Science Editors:

Wang P. Development of Nanoparticle-based Platforms for Potential Applications in Biosensing and Therapeutics. [Doctoral Dissertation]. University of Cincinnati; 2017. Available from: http://rave.ohiolink.edu/etdc/view?acc_num=ucin151186771296011

University of Cincinnati

12. Ding, Rui. Enhanced Singlet Oxygen Production from Metal Nanoparticle Based Hybrid Photosensitizers.

Degree: PhD, Arts and Sciences: Chemistry, 2016, University of Cincinnati

URL: http://rave.ohiolink.edu/etdc/view?acc_num=ucin1447690607

► In this dissertation, we explored the syntheses and characterizations of water dispersible hybrid photosensitizers by combining metal nanoparticles and organic photosensitizers. In the first project,… (more)

▼ In this dissertation, we explored the syntheses and characterizations of water dispersible hybrid photosensitizers by combining metal nanoparticles and organic photosensitizers. In the first project, we reported a novel metal enhanced singlet oxygen production and fluorescence quenching phenomenon based on the conjugates between gold nanoparticles and rose bengal. When rose bengal covalently conjugated to the surface of average ~ 50 nm gold nanoparticles, the fluorescence was dramatically quenched; however, singlet oxygen production was implied to be enhanced by measuring its phosphorescence at ~ 1280 nm. Singlet oxygen quantum yield of the novel hybrid photosensitizer was calculated as to be 0.75 by using rose bengal as reference photosensitizer.In the second project, we discussed the employing of carboxylic modified silver nanoparticles, 6-mercaptohexanoic acid stabilized silver nanoparticles, to conjugate with toluidine blue O to form a novel hybrid photosensitizer. The silver nanoparticles were prepared by a modified Brust’s method with an average size ~ 5 nm. The covalent conjugation between silver and toluidine blue O quenched the fluorescence and enhanced the singlet oxygen production according to the spectroscopic results. The presence of singlet oxygen and the enhancement of singlet oxygen production were demonstrated by the photodecomposition of 1,3-diphenylisobenzofuran. Singlet oxygen quantum yield of the hybrid photosensitizer was calculated as 0.78.In the third project, thiol-modified amphiphilic block copolymer, poly(N-isopropylacrylamide-block-styrene), was synthesized and characterized. Then, a novel hybrid photosensitizer based on poly(N-isopropylacrylamide-block-styrene) stabilized silver nanoparticles and entrapped hydrophobic photosensitizer (hematoporphyrin) was prepared. The water dispersible hybrid photosensitizer demonstrated enhanced singlet oxygen production with broadened excitation profile by monitoring phosphorescence at ~1280 nm. The photodynamic inactivation of the hybrid photosensitizer on Staphylococcus epidermidis and Escherichia coli showed enhanced killing efficacy up to ~5 orders under white light or NIR light illumination. The hybrid photosensitizer with concentration used in photodynamic inactivation assays showed low toxicity to Hela cells under ambient light condition. Those results demonstrate great potential of such hybrid photosensitizers for photodynamic inactivation and photodynamic therapy applications.In summary, we stated the preparation and analyses of the three hybrid photosensitizers with potential application in photodynamic therapy. Advisors/Committee Members: Zhang, Peng (Committee Chair).

Subjects/Keywords: Chemistry; singlet oxygen; metal nanoparticles; photosensitizer; photodynamic therapy

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APA (6^th Edition):

Ding, R. (2016). Enhanced Singlet Oxygen Production from Metal Nanoparticle Based Hybrid Photosensitizers. (Doctoral Dissertation). University of Cincinnati. Retrieved from http://rave.ohiolink.edu/etdc/view?acc_num=ucin1447690607

Chicago Manual of Style (16^th Edition):

Ding, Rui. “Enhanced Singlet Oxygen Production from Metal Nanoparticle Based Hybrid Photosensitizers.” 2016. Doctoral Dissertation, University of Cincinnati. Accessed January 23, 2021. http://rave.ohiolink.edu/etdc/view?acc_num=ucin1447690607.

MLA Handbook (7^th Edition):

Ding, Rui. “Enhanced Singlet Oxygen Production from Metal Nanoparticle Based Hybrid Photosensitizers.” 2016. Web. 23 Jan 2021.

Vancouver:

Ding R. Enhanced Singlet Oxygen Production from Metal Nanoparticle Based Hybrid Photosensitizers. [Internet] [Doctoral dissertation]. University of Cincinnati; 2016. [cited 2021 Jan 23]. Available from: http://rave.ohiolink.edu/etdc/view?acc_num=ucin1447690607.

Council of Science Editors:

Ding R. Enhanced Singlet Oxygen Production from Metal Nanoparticle Based Hybrid Photosensitizers. [Doctoral Dissertation]. University of Cincinnati; 2016. Available from: http://rave.ohiolink.edu/etdc/view?acc_num=ucin1447690607

University of Dayton

13. Li, Kaiyu. Supramolecular Ruthenium(II) and Osmium(II) Complexes: Synthesis, Characterization, DNA Binding and DNA Photocleavage.

Degree: MS(M.S.), Chemistry, 2017, University of Dayton

URL: http://rave.ohiolink.edu/etdc/view?acc_num=dayton1513235232385279

► Two new bridging ligands have been synthesized by combining substituted benzaldehydes with phenanthrolinopyrrole (php), resulting in new polyazine bridging ligands. The ligands have been characterized… (more)

▼ Two new bridging ligands have been synthesized by combining substituted benzaldehydes with phenanthrolinopyrrole (php), resulting in new polyazine bridging ligands. The ligands have been characterized by 1H NMR, mass spectroscopy, and elemental analysis. These new ligands display ϖ-ϖ* transitions above 500 nm with modest molar absorptivities. Upon excitation at the ligand-centered charge-transfer transition, weak emission with a maximum wavelength of 612 nm is observed. When coordinated to two ruthenium (II) bis-(2,2’-bipyridine) groups, the new bimetallic complexes generated give an overall 4+ charge. The electronic transitions of the bimetallic ruthenium (II) complexes display traditional ϖ-ϖ* transitions at 287 nm and metal-to-ligand charge-transfer transitions at 452 nm with molar absorptivities greater than 30000 M^-1 cm^-1. Oxidation of the ruthenium (II) metal centers to ruthenium (III) occurs at potentials above 1.4 V versus the Ag/AgCl reference electrode. Spectroscopic and electrochemical measurements indicate that the ruthenium (II) moieties behave independently. Both complexes are water-soluble and show the ability to photo-nick plasmid DNA when irradiated with low-energy light above 550 nm. In addition, one of the complexes, [Ru(bpy)₂php]₂Van⁴⁺, shows the ability to linearize plasmid DNA and gives evidence, by gel electrophoresis, of photoinduced binding to plasmid DNA.Coordination of two Osmium(II) bis-(bipyridine) complexes to the peripheral phenanthroline of (4-hydroxy-3- methoxyphenyl)diphenanthropyrromethane ligand yields the bimetallic Os(II) complex. The spectroscopic properties are similar to those of [Os(bpy)₃]²⁺ with ligand-centered ϖ-ϖ* transitions in the UV region of the spectrum and three metal-to-ligand charge-transfer (¹MLCT) transitions in the visible region. A broad ³MLCT is observed stretching from 550 to 700 nm with modest intensity. Binding studies with calf thymus DNA (ctDNA) show binding constants as high as 10⁵ M^–1 indicating a strong interaction of the complex with DNA. When aqueous solutions of the complex and plasmid DNA are irradiated with low-energy light for brief periods of time complete photo- cleavage of the DNA is observed. Studies indicate that the mechanism of this photoreaction requires molecular oxygen and results from both the formation of ¹O₂ and oxygen radicals offering multiple modes for DNA destruction. Advisors/Committee Members: Swavey, Shawn (Committee Chair).

Subjects/Keywords: Chemistry; Photosensitizer; Ruthenium; Osmium; Bimetallic complexes; DNA Photocleavage

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APA (6^th Edition):

Li, K. (2017). Supramolecular Ruthenium(II) and Osmium(II) Complexes: Synthesis, Characterization, DNA Binding and DNA Photocleavage. (Masters Thesis). University of Dayton. Retrieved from http://rave.ohiolink.edu/etdc/view?acc_num=dayton1513235232385279

Chicago Manual of Style (16^th Edition):

Li, Kaiyu. “Supramolecular Ruthenium(II) and Osmium(II) Complexes: Synthesis, Characterization, DNA Binding and DNA Photocleavage.” 2017. Masters Thesis, University of Dayton. Accessed January 23, 2021. http://rave.ohiolink.edu/etdc/view?acc_num=dayton1513235232385279.

MLA Handbook (7^th Edition):

Li, Kaiyu. “Supramolecular Ruthenium(II) and Osmium(II) Complexes: Synthesis, Characterization, DNA Binding and DNA Photocleavage.” 2017. Web. 23 Jan 2021.

Vancouver:

Li K. Supramolecular Ruthenium(II) and Osmium(II) Complexes: Synthesis, Characterization, DNA Binding and DNA Photocleavage. [Internet] [Masters thesis]. University of Dayton; 2017. [cited 2021 Jan 23]. Available from: http://rave.ohiolink.edu/etdc/view?acc_num=dayton1513235232385279.

Council of Science Editors:

Li K. Supramolecular Ruthenium(II) and Osmium(II) Complexes: Synthesis, Characterization, DNA Binding and DNA Photocleavage. [Masters Thesis]. University of Dayton; 2017. Available from: http://rave.ohiolink.edu/etdc/view?acc_num=dayton1513235232385279

The Ohio State University

14. Shi, Xiaofeng. Time-Resolved Spectroscopic Studies of the Photochemistry of riboflavin, aromatic N-Oxides and the absolute reactivity of hydroxyl radical.

Degree: PhD, Chemistry, 2005, The Ohio State University

URL: http://rave.ohiolink.edu/etdc/view?acc_num=osu1126795561

► Both nanosecond and ultrafast laser flash photolysis with UV-visible and infrared detection were used to observe the transient species generated photochemically from a number of… (more)

▼ Both nanosecond and ultrafast laser flash photolysis with UV-visible and infrared detection were used to observe the transient species generated photochemically from a number of photosensitizers. The reactions of these transient species were monitored spectroscopically with the aid of theoretical computation.In the study of photochemical reactions of riboflavin and nucleosides, it was found that triplet riboflavin can be quenched by a silylated guanosine derivative. TRIR spectroscopy demonstrated that a hydroflavin radical is formed by an electron transfer-proton transfer mechanism. This sequential electron transfer-proton transfer between triplet riboflavin and guanosine derivative provides the direct observation of the photoinduced oxidative damage of riboflavin to the DNA nucleobase. The triplet states of isoquinoline N-oxide and benzocinnoline N-oxide react sluggishly with electron, proton and hydrogen atom donors. These triplets will react with hydroquinone by hydrogen atom transfer (proton coupled electron transfer). Triplet 4-nitroquinoline N-oxide reacts readily with electron donors to from the radical anions as previously reported. The radical anion is protonated on the oxygen atom of the N-oxide group to from a neutral radical. The three N-oxides of this study are not expected to serve as photochemical sources of hydroxyl radical. Singlet states of tirapazamine and desoxytirapazamine were identified by picosecond time-resolved absorption spectroscopy. The lifetimes of the S1 states and fluorescence quantum yields of aromatic N-oxides were found to be controlled by reversible cyclization to an oxaziridine. The S1 states of TPZ and dTPZ are reduced to radical anions by KSCN, KI and NaN3. Using LFP-based methodology, we have determined the rate coefficients for the reaction of hydroxyl radical with a number of monocyclic and polycyclic aromatic hydrocarbons in acetonitrile. We observed the reactivities of hydroxyl radical in acetonitrile. For simple aromatic hydrocarbons, the predominant reaction pathway in acetonitrile is the addition of the hydroxyl radical to the aromatic ring, rather than hydrogen-atom abstraction from the phenyl or benzylic C-H positions. Structure-reactivity analysis, based upon frontier molecular orbital and state correlation models indicate that charge-transfer interactions between hydroxyl radical and a given arene play an important role in the stabilization of the transition state for the reaction. Alpha-Alkoxy and hydroxy radicals were generated through thermal and photochemical reactions. Both the product analysis of the thermal reaction and the direct observation of the transient species involved suggest that alpha-hydroxy radical can very efficiently react with TPZ to form the TPZ-H radical, probably through a direct hydrogen atom exchange between TPZ and ketyl radicals. Alpha-alkoxy radicals can not proceed through this mechanism. Advisors/Committee Members: Platz, Matthew (Advisor).

Subjects/Keywords: Photosensitizer; Riboflavin; Tirapazamine; Hydroxyl radical

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APA (6^th Edition):

Shi, X. (2005). Time-Resolved Spectroscopic Studies of the Photochemistry of riboflavin, aromatic N-Oxides and the absolute reactivity of hydroxyl radical. (Doctoral Dissertation). The Ohio State University. Retrieved from http://rave.ohiolink.edu/etdc/view?acc_num=osu1126795561

Chicago Manual of Style (16^th Edition):

Shi, Xiaofeng. “Time-Resolved Spectroscopic Studies of the Photochemistry of riboflavin, aromatic N-Oxides and the absolute reactivity of hydroxyl radical.” 2005. Doctoral Dissertation, The Ohio State University. Accessed January 23, 2021. http://rave.ohiolink.edu/etdc/view?acc_num=osu1126795561.

MLA Handbook (7^th Edition):

Shi, Xiaofeng. “Time-Resolved Spectroscopic Studies of the Photochemistry of riboflavin, aromatic N-Oxides and the absolute reactivity of hydroxyl radical.” 2005. Web. 23 Jan 2021.

Vancouver:

Shi X. Time-Resolved Spectroscopic Studies of the Photochemistry of riboflavin, aromatic N-Oxides and the absolute reactivity of hydroxyl radical. [Internet] [Doctoral dissertation]. The Ohio State University; 2005. [cited 2021 Jan 23]. Available from: http://rave.ohiolink.edu/etdc/view?acc_num=osu1126795561.

Council of Science Editors:

Shi X. Time-Resolved Spectroscopic Studies of the Photochemistry of riboflavin, aromatic N-Oxides and the absolute reactivity of hydroxyl radical. [Doctoral Dissertation]. The Ohio State University; 2005. Available from: http://rave.ohiolink.edu/etdc/view?acc_num=osu1126795561

15. Fernanda Bettanin. Estudo químico-quântico de corantes catiônico e aniônicos para aplicação em terapia fotodinâmica.

Degree: 2011, Universidade Federal do ABC

URL: http://tede.ufabc.edu.br/tde_busca/arquivo.php?codArquivo=157

►

The present work studies the dyes Methylene Blue, Toluidine Blue, Eosin Y, Erytrosin B, Fluorescein and Rose Bengal using quantum-chemical calculations, aiming to propose an… (more)

▼

The present work studies the dyes Methylene Blue, Toluidine Blue, Eosin Y, Erytrosin B, Fluorescein and Rose Bengal using quantum-chemical calculations, aiming to propose an appropriate Density Functional Theory-based methodology and to understand the mechanisms of action of those dyes in photodynamic therapy. Structures in singlet and triplet states were optimized with B3LYP and mPW1PW91 (PW91) functionals and DGTZVP basis set. Solvation (etanol and aqueous solution) was simulated by IEFPCM model. Absorption spectra were calculated with TD-DFT (B3LYP and PW91) and ZINDO methods and emission spectra were simulated with TD-DFT (B3LYP) and MP2 methods. To study the formation of dimers, we have used the BLYP functional corrected to provide the dispersion energy with DCACP. There is no significant difference in geometry, total energy and dipole moment calculated with B3LYP or PW91 functionals. The semi-empirical method ZINDO, parametrized to reproduce absorption spectra, presented erros of 11% in dyes wavelength if compared to experimental data, while TD-DFT presented errors of 18%. The introduction of solvent effect improved TD-DFT absorption spectra in 4%. It was also found that electron transfer should occur easily than hydrogen atom transfer if mechanism type l of photodynamic therapy. Type ll mechanism was not verified because the calculation scheme does not apply to the studied molecules. The formation of dimers was confirmed in the presence of ethanol and aqueous solution.

O presente trabalho estuda por meio de cálculos químico-quânticos os corantes Azul de Metileno, Azul de Toluidina, Eosina Y, Eritrosina B, Fluoresceína e Rosa de Bengala, visando encontrar uma metodologia apropriada baseada na Teoria do Funcional da Densidade e entender os mecanismos de ação destes em terapia fotodinâmica. Foram otimizadas as estruturas com os funcionais B3LYP e mPW1PW91 (PW91) e a base DGTZVP. O solvente (etanol e água) foi simulado pelo modelo de solvatação IEFPCM, no estado singleto e tripleto. Foram calculados os espectros de absorção com os métodos TD- DFT (B3LYP e PW91) e ZINDO e os espectros de emissão com os métodos TD-DFT (B3LYP) e MP2. Para o estudo da formação de dímeros foi utilizado o funcional BLYP com correções para estimar a energia de dispersão, os chamados DCACP ́s. Não houve diferença significativa na geometria, energia total e momento de dipolo cálculados com os funcionais B3LYP e PW91. O método semi-empírico ZINDO, parametrizado para reproduzir espectros de absorção, forneceu comprimentos de onda 11% em média menores em relação aos valores experimentais, enquanto com TD-DFT os erros foram de cerca de 18%. A introdução do efeito do solvente melhorou os espectros de absorção calculados com TD- DFT em 4%. Verificou-se que no mecanismo tipo l da terapia fotodinâmica, é mais provável a transferência de elétrons que de hidrogênios. O mecanismo tipo ll não foi possível verificar, pois o esquema utilizado não se aplica ao sistema estudado. A formação de dímeros foi confirmada na presença dos solventes etanol…

Advisors/Committee Members: Paula homem de Mello.

Subjects/Keywords: fotossensibilizador; corantes; DFT; DCACPs; terapia fotodinânica; dyes; photosensitizer; DFT; DCACPs; photodynamic therapy; QUIMICA; corantes; fotossensibilizador; DFT; DCACPs; terapia fotodinânica; dyes; photosensitizer; DFT; DCACPs; photodynamic therapy

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Bettanin, F. (2011). Estudo químico-quântico de corantes catiônico e aniônicos para aplicação em terapia fotodinâmica. (Thesis). Universidade Federal do ABC. Retrieved from http://tede.ufabc.edu.br/tde_busca/arquivo.php?codArquivo=157

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Bettanin, Fernanda. “Estudo químico-quântico de corantes catiônico e aniônicos para aplicação em terapia fotodinâmica.” 2011. Thesis, Universidade Federal do ABC. Accessed January 23, 2021. http://tede.ufabc.edu.br/tde_busca/arquivo.php?codArquivo=157.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Bettanin, Fernanda. “Estudo químico-quântico de corantes catiônico e aniônicos para aplicação em terapia fotodinâmica.” 2011. Web. 23 Jan 2021.

Vancouver:

Bettanin F. Estudo químico-quântico de corantes catiônico e aniônicos para aplicação em terapia fotodinâmica. [Internet] [Thesis]. Universidade Federal do ABC; 2011. [cited 2021 Jan 23]. Available from: http://tede.ufabc.edu.br/tde_busca/arquivo.php?codArquivo=157.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Bettanin F. Estudo químico-quântico de corantes catiônico e aniônicos para aplicação em terapia fotodinâmica. [Thesis]. Universidade Federal do ABC; 2011. Available from: http://tede.ufabc.edu.br/tde_busca/arquivo.php?codArquivo=157

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

NSYSU

16. Chu, Ya-lan. Photochemical Reactivity between Graphene and Organic Peroxides.

Degree: Master, Chemistry, 2016, NSYSU

URL: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0629116-135623

► Graphene is a two-dimensional material composed of carbon atoms that are arranged in a single-layer hexagonal crystal lattice form. Due to its complete conjugated ï°… (more)

▼ Graphene is a two-dimensional material composed of carbon atoms that are arranged in a single-layer hexagonal crystal lattice form. Due to its complete conjugated ï° system, graphene is chemically stable and thus has high potential in a variety of applications. Therefore, research on graphene has become popular and challenging in recent years. Previous studies have indicated that organic peroxides could undergo photolysis upon irradiation. In this research, we select di-tert butyl peroxide (DTBP) and tert-butyl peroxybenzoate (TBPB) as the radical initiator, respectively, and 365 nm LED as the light source to investigate the thermochemistry and photochemistry between graphene and adsorbed peroxides. Experiments were carried out in our ultrahigh vacuum system and in situ surface analytic techniques, including temperature programmed desorption (TPD) and reflection adsorption infrared spectroscopy (RAIRS), were employed. The interaction of DTBP with the surface of graphene showed that only the monolayer desorption was observed at 194 K. Neither thermochemistry nor photochemistry reactions happened.The results of reacting TBPB with the surface of graphene showed that TBPB was found to be depleted by 1 hour UV irradiation in the PITPD measurement. RAIR spectra showed that both carbonyl stretching vibration at 1765 cm-1 and peroxyl stretching vibration at 835 cm-1 almost disappeared after 1 hour irradiation, but two new peaks (1210 and 930 cm -1) simultaneously emerged. Hence, we confirmed TBPB underwent photolysis along with the formation of radical intermediates. Since all IR features faded out above 300 K, covalent bond formation between the expected photochemically generated phenyl or methyl radicals and the graphene carbons didnât occurred. Instead, these radicals underwent disproportionation and thus formed benzene, tert-butanol and isobutylene epoxide as the final products. All products generated desorbed from the graphene surface before 200 K. TBPB itself does not absorb 365 nm wavelength of light but graphene does. Photoexcited graphene serves as a photosensitizer responsible for delivering hot electrons to the LUMO of TBPB. Meanwhile, an electron within the TBPB HOMO transferred to graphene, the energy transfer resulted from the electron-exchange triggered formation of ground state graphene and excited state TBPB. The excited state TBPB was decomposed via homolytic cleavage of O-O bond to render radicals. Advisors/Committee Members: Jean-Ho Chu (chair), Chao-Ming Chiang (committee member), Chun-hu Chen (chair).

Subjects/Keywords: electron exchange; photosensitizer; CVD grown graphene; disproportionation; ultrahigh vacuum system; energy transfer

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Chu, Y. (2016). Photochemical Reactivity between Graphene and Organic Peroxides. (Thesis). NSYSU. Retrieved from http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0629116-135623

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Chu, Ya-lan. “Photochemical Reactivity between Graphene and Organic Peroxides.” 2016. Thesis, NSYSU. Accessed January 23, 2021. http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0629116-135623.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Chu, Ya-lan. “Photochemical Reactivity between Graphene and Organic Peroxides.” 2016. Web. 23 Jan 2021.

Vancouver:

Chu Y. Photochemical Reactivity between Graphene and Organic Peroxides. [Internet] [Thesis]. NSYSU; 2016. [cited 2021 Jan 23]. Available from: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0629116-135623.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Chu Y. Photochemical Reactivity between Graphene and Organic Peroxides. [Thesis]. NSYSU; 2016. Available from: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0629116-135623

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Mississippi State University

17. Suwatpipat, Kullatat. STUDIES OF THE UPCONVERSION OF LIGHT BY Ru(II) COMPLEXES AS PHOTOSENSITIZERS WITH ANTHRACENE DERIVATIVES AS EMITTERS.

Degree: PhD, Chemistry, 2010, Mississippi State University

URL: http://sun.library.msstate.edu/ETD-db/theses/available/etd-06302010-110612/ ;

► High-energy light was generated from lower-energy photons through an upconversion process using a mixture of a photosensitizer and an emitter. Factors that influence… (more)

Subjects/Keywords: Upconversion; Ruthenium complex; Photosensitizer; Anthracene; Emitter

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Suwatpipat, K. (2010). STUDIES OF THE UPCONVERSION OF LIGHT BY Ru(II) COMPLEXES AS PHOTOSENSITIZERS WITH ANTHRACENE DERIVATIVES AS EMITTERS. (Doctoral Dissertation). Mississippi State University. Retrieved from http://sun.library.msstate.edu/ETD-db/theses/available/etd-06302010-110612/ ;

Chicago Manual of Style (16^th Edition):

Suwatpipat, Kullatat. “STUDIES OF THE UPCONVERSION OF LIGHT BY Ru(II) COMPLEXES AS PHOTOSENSITIZERS WITH ANTHRACENE DERIVATIVES AS EMITTERS.” 2010. Doctoral Dissertation, Mississippi State University. Accessed January 23, 2021. http://sun.library.msstate.edu/ETD-db/theses/available/etd-06302010-110612/ ;.

MLA Handbook (7^th Edition):

Suwatpipat, Kullatat. “STUDIES OF THE UPCONVERSION OF LIGHT BY Ru(II) COMPLEXES AS PHOTOSENSITIZERS WITH ANTHRACENE DERIVATIVES AS EMITTERS.” 2010. Web. 23 Jan 2021.

Vancouver:

Suwatpipat K. STUDIES OF THE UPCONVERSION OF LIGHT BY Ru(II) COMPLEXES AS PHOTOSENSITIZERS WITH ANTHRACENE DERIVATIVES AS EMITTERS. [Internet] [Doctoral dissertation]. Mississippi State University; 2010. [cited 2021 Jan 23]. Available from: http://sun.library.msstate.edu/ETD-db/theses/available/etd-06302010-110612/ ;.

Council of Science Editors:

Suwatpipat K. STUDIES OF THE UPCONVERSION OF LIGHT BY Ru(II) COMPLEXES AS PHOTOSENSITIZERS WITH ANTHRACENE DERIVATIVES AS EMITTERS. [Doctoral Dissertation]. Mississippi State University; 2010. Available from: http://sun.library.msstate.edu/ETD-db/theses/available/etd-06302010-110612/ ;

18. Vollet Filho, José Dirceu. \"Identificação e quantificação de fotossensibilizador em tecido hepático por espectroscopia de fluorescência e sua importância na terapia fotodinâmica\".

Degree: Mestrado, Física Aplicada, 2007, University of São Paulo

URL: http://www.teses.usp.br/teses/disponiveis/76/76132/tde-28032007-180658/ ;

►

A Terapia Fotodinâmica (TFD) é uma técnica que provoca dano celular pela ação de um fotossensibilizador (FS), com seletividade de localização em tecido tumoral; a… (more)

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A Terapia Fotodinâmica (TFD) é uma técnica que provoca dano celular pela ação de um fotossensibilizador (FS), com seletividade de localização em tecido tumoral; a luz que, absorvida pelo FS, leva-o a um estado tripleto metaestável; e oxigênio molecular, o qual recebe a energia absorvida pelo FS, passando a um estado singleto de alta capacidade oxidativa. A técnica é bem sucedida no tratamento de lesões como câncer, mas enfrenta, entretanto, dificuldades para a determinação de sua dosimetria. Uma delas é a quantificação da distribuição do FS no tecido tratado. Este trabalho tem três objetivos: a obtenção de informação quantitativa por espectros de fluorescência de fluoróforos em meios turvos; a demonstração da distribuição do FS Photogem® em fígados sadios de ratos Wistar e suas implicações na dosimetria; e a melhoria de um dos modelos existentes para previsão da profundidade de necrose (Ynec), importante parâmetro no estudo da TFD. Realizaram-se os experimentos em três fases: na primeira, tentou-se reconstruir o espectro do fígado sadio a partir de uma composição de espectros isolados de fluoróforos endógenos do fígado. Na segunda, realizaram-se estudos com corantes alimentícios Coralim-Mix® nas cores azul, verde e vermelho e com corantes Exciton® (Coumarin-480 e LDS-722) in vitro e in vivo, visando identificar os espectros dos corantes em misturas com meios turvos e entre eles. Na terceira, aplicou-se Photogem® em ratos Wistar e se coletou a fluorescência do FS nos fígados, relacionando-se a variação na intensidade de fluorescência com a concentração de FS presente e com perfis de necrose obtidos por TFD. Aplicou-se, por fim, os resultados obtidos na melhoria do modelo para previsão da Ynec. A reconstrução dos espectros não foi bem sucedida, tal qual a recuperação dos espectros dos corantes. Os resultados mostraram que muitos fatores contribuem para a distorção da fluorescência coletada, e que a informação obtida é prejudicada se estes forem ignorados. Verificou-se que a distribuição do FS não é homogênea num órgão fotossensibilizado. Obteve-se uma função para distribuição do FS no tecido e, através dela, foi possível melhorar o modelo para a previsão da Ynec. Observou-se que a turbidez do meio afeta de maneira complexa a coleta de fluorescência, criando obstáculos para a quantificação direta de fluoróforos nele inseridos. Fica evidente a necessidade do aprofundamento dos estudos sobre a interação da luz com as partículas dos meios turvos para remover as distorções geradas por estas. Demonstrou-se ainda a importância do mapeamento da distribuição do FS num tecido fotossensibilizado como parte da dosimetria da TFD, e que a espectroscopia de fluorescência é forte candidata à técnica mais apropriada para este mapeamento, desde que dominados os obstáculos à coleta de fluorescência.

Photodynamic Therapy (PDT) is a technique that implies in cell damage by the action of a photosensitizer (PS) with tumor tissue localization selectivity; light at PS absorption spectrum wavelengths, which leads the PS to a…

Advisors/Committee Members: Bagnato, Vanderlei Salvador.

Subjects/Keywords: dosimetria; dosimetry; fígado; fluorescence; fluorescência; fotossensibilizador; liver; PDT; PDT; photodinamic therapy; photosensitizer; terapia fotodinâmica; TFD

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Vollet Filho, J. D. (2007). \"Identificação e quantificação de fotossensibilizador em tecido hepático por espectroscopia de fluorescência e sua importância na terapia fotodinâmica\". (Masters Thesis). University of São Paulo. Retrieved from http://www.teses.usp.br/teses/disponiveis/76/76132/tde-28032007-180658/ ;

Chicago Manual of Style (16^th Edition):

Vollet Filho, José Dirceu. “\"Identificação e quantificação de fotossensibilizador em tecido hepático por espectroscopia de fluorescência e sua importância na terapia fotodinâmica\".” 2007. Masters Thesis, University of São Paulo. Accessed January 23, 2021. http://www.teses.usp.br/teses/disponiveis/76/76132/tde-28032007-180658/ ;.

MLA Handbook (7^th Edition):

Vollet Filho, José Dirceu. “\"Identificação e quantificação de fotossensibilizador em tecido hepático por espectroscopia de fluorescência e sua importância na terapia fotodinâmica\".” 2007. Web. 23 Jan 2021.

Vancouver:

Vollet Filho JD. \"Identificação e quantificação de fotossensibilizador em tecido hepático por espectroscopia de fluorescência e sua importância na terapia fotodinâmica\". [Internet] [Masters thesis]. University of São Paulo; 2007. [cited 2021 Jan 23]. Available from: http://www.teses.usp.br/teses/disponiveis/76/76132/tde-28032007-180658/ ;.

Council of Science Editors:

Vollet Filho JD. \"Identificação e quantificação de fotossensibilizador em tecido hepático por espectroscopia de fluorescência e sua importância na terapia fotodinâmica\". [Masters Thesis]. University of São Paulo; 2007. Available from: http://www.teses.usp.br/teses/disponiveis/76/76132/tde-28032007-180658/ ;

19. Natal, Aline Martins Duboc. Influência do pH na interação do Photofrin®, Photogem® e Photosan® com DMPC e lipoproteína de baixa densidade.

Degree: Mestrado, Química Analítica, 2007, University of São Paulo

URL: http://www.teses.usp.br/teses/disponiveis/75/75132/tde-27032008-110609/ ;

►

O efeito do fotossensibilizador na estrutura biológica não é apenas influenciado por suas propriedades fotofísicas, mas também por sua interação específica com biosistemas.Além disso, a… (more)

▼

O efeito do fotossensibilizador na estrutura biológica não é apenas influenciado por suas propriedades fotofísicas, mas também por sua interação específica com biosistemas.Além disso, a localização do fotossensibilizador no tecido tumoral é um importante fator que resulta em diferentes mecanismos de destruição do tumor. Muitos fotossensibilizadores, após administração sistêmica, se ligam às proteínas plasmáticas e com isso são distribuídos em diferentes sítios no organismo. Os fotossensibilizadores hidrofílicos são largamente transportados por albuminas e globilinas e se acumulam preferencialmente no estroma vascular dos tumores. Entretanto, fotossensibilizadores mais hidrofóbicos se ligam às lipoproteínas, principalmente LDL, que promove a entrada do FS na célula através de endocitose mediado por receptor. Sendo assim, a localização do FS depende de sua ligação com as deferentes proteínas plasmáticas, sua farmacocinética e também é influenciada pela diferença entre o tecido normal e tumoral. O tecido tumoral tem pH mais baixo e maior expressão de receptores de LDL do que os tecidos normais, aumentando a seletividade dos FSs as células tumorais. A incorporação de FS hidrofóbicos em lipossomas para a administração sistêmica pode realçar ao transporte deste pelas lipoproteínas. No presente trabalho estudou-se a influência do pH na interação de fotossensibilizadores com lipossomas de DMPC e LDL. Os fotossensibilizadores utilizados nesse estudo foram Photofrin®, Photogem® e Photosan® que são derivados de hematoporfirinas. A metodologia empregada constitui de variação das concentrações de DMPC e LDL para os seguintes valores de pHs 5,0; 7,4 e 9,0, esse último pH utilizou-se somente para DMPC. O complexo FS - DMPC foi obtido por incubação dos FSs na concentração de 10 micro g.mL-1 com diferentes concentrações de DMPC (0 a 400 micro M) por trinta minutos no escuro. Isolou-se o LDL do plasma humano por ultracentrifugação por gradiente de densidade. Após a separação, o complexo FS - LDL foi obtido por incubação (12 horas no escuro) do FS na concentração 10 micro g.mL-1 com diferentes concentrações de LDL (0 a 0,04 micro M). O comportamento desses complexos foi analisado por espectroscopia de absorção ótica e por espectroscopia de fluorescência.

The effect of a photosensitizing compound on biological structures is governed not only by its photophysical properties but also by the specificity of its interaction with biosystems. Moreover, localization of the photosensitizer in the tumor tissue is an important factor affecting the outcome as well as mechanism leading to tumor destruction. Following administration, most photosensitizers are bound to blood components and delivered to different sites in the organism. It is generally accepted that hydrophilic photosensitizers are largely transported by albumins and globulins and mainly accumulate in the vascular stroma of tumors. More hydrophobic sensitizers are bound to lipoproteins, which promote drug internalization by cells through endocytosis of the lipoprotein carrier. In…

Advisors/Committee Members: Imasato, Hidetake.

Subjects/Keywords: DMPC; DMPC; fotossensibilizador; LDL; LDL; lipoprotein; lipoproteina; Photofrin; Photofrin; Photogem; Photogem; Photosan; Photosan; photosensitizer

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Natal, A. M. D. (2007). Influência do pH na interação do Photofrin®, Photogem® e Photosan® com DMPC e lipoproteína de baixa densidade. (Masters Thesis). University of São Paulo. Retrieved from http://www.teses.usp.br/teses/disponiveis/75/75132/tde-27032008-110609/ ;

Chicago Manual of Style (16^th Edition):

Natal, Aline Martins Duboc. “Influência do pH na interação do Photofrin®, Photogem® e Photosan® com DMPC e lipoproteína de baixa densidade.” 2007. Masters Thesis, University of São Paulo. Accessed January 23, 2021. http://www.teses.usp.br/teses/disponiveis/75/75132/tde-27032008-110609/ ;.

MLA Handbook (7^th Edition):

Natal, Aline Martins Duboc. “Influência do pH na interação do Photofrin®, Photogem® e Photosan® com DMPC e lipoproteína de baixa densidade.” 2007. Web. 23 Jan 2021.

Vancouver:

Natal AMD. Influência do pH na interação do Photofrin®, Photogem® e Photosan® com DMPC e lipoproteína de baixa densidade. [Internet] [Masters thesis]. University of São Paulo; 2007. [cited 2021 Jan 23]. Available from: http://www.teses.usp.br/teses/disponiveis/75/75132/tde-27032008-110609/ ;.

Council of Science Editors:

Natal AMD. Influência do pH na interação do Photofrin®, Photogem® e Photosan® com DMPC e lipoproteína de baixa densidade. [Masters Thesis]. University of São Paulo; 2007. Available from: http://www.teses.usp.br/teses/disponiveis/75/75132/tde-27032008-110609/ ;

20. Aline Alves Ramos. Obtenção e caracterização de ftalocianinas com substituintes quirais com potencial aplicação em terapia fotodinâmica.

Degree: 2012, Universidade Federal do ABC

URL: http://tede.ufabc.edu.br/tde_busca/arquivo.php?codArquivo=262

►

The main objective of this project is the synthesis and characterization of new phthalocyanines with chiral substituents to improve their photophysical and photochemical properties for… (more)

▼

The main objective of this project is the synthesis and characterization of new phthalocyanines with chiral substituents to improve their photophysical and photochemical properties for use as photosensitizers in Photodynamic Therapy. Three new phthalocyanines were synthesized and characterized, one with the racemic mixture of 1-(4-bromophenyl)- ethanol, and the other two with the pure enantiomers, (R) - 1 - ( 4-bromophenyl)-ethanol and (S)-1-(4-bromophenyl)-ethanol. The compounds were characterized by 1H NMR spectroscopy and mass spectrometry. The photophysical and photochemical characteristics were determined by analysis of UV-Vis absorption, excitation and emission spectra, and the determination of the aggregation constant values. The quantum yield of generation of reactive species was determined by the indirect method, using the diphenylisobenzofuran (DPBF) as suppressor. The results showed three different values of aggregation constant for phthalocyanines sinthesized, with the macrocycle modify with enantiomeric (R) alcohol with the lowest one. The results also showed that this same phthalocyanines has a bigger quantum yield of generation of reactive oxygen species than the others. These two results are consistent with the fact that a lower aggregation results in a higher quantum yield in the generation of reactive oxygen species, and that the geometry of the substituents studied is directly related to the behavior of phthalocyanines. With respect to biological results, established by analysis of fluorescence microscopy, the three phthalocyanines interact with the cell line studied, MCF7 (breast epithelial tissue). However, through the images, was not possible to define the exact content of each compound that interact with the cell.

O objetivo deste projeto é a síntese e caracterização de novas ftalocianinas com substituintes quirais nas posições periféricas do anel, visando a melhoria das propriedades fotoquímicas e fotofísicas destes compostos para a utilização como fotossensibilizadores em Terapia Fotodinâmica. Foram sintetizadas e caracterizadas três novas ftalocianinas, sendo uma delas modificada com a mistura racêmica do composto quiral 1-(4-bromofenil)-etanol, e os outros dois macrociclos modificados com os enantiômeros puros, ou seja, o (R)- 1-(4- bromofenil)-etanol e o (S)- 1-(4-bromofenil)-etanol. Os compostos foram caracterizados por espectroscopia 1H RMN e espectrometria de massas, e suas características fotofísicas e fotoquímicas foram determinadas por análise dos espectros de absorção na região do UVVis, espectros de excitação e emissão e pela determinação de valores como a constante de agregação e outros. Ainda, o rendimento quântico de geração de espécies reativas foi determinado pelo método indireto, empregando o difenilisobenzofurano (DPBF) como supressor, bem como foram avaliadas a interação de cada um dos compostos com células do tipo MCF7 por microscopia de fluorescência. Nos resultados constatou-se que as três ftalocianinas sintetizadas apresentam valores diferentes de constante de…

Advisors/Committee Members: Vani Xavier de Oliveira Júnior, Anderson Orzari Ribeiro.

Subjects/Keywords: QUIMICA; Ftalocianianinas; Terapia Fotodinâmica; Fotossensibilizador; Fotoquímica; Síntese; Phthalocyanine; Photodynamic Therapy; Photosensitizer; Photochemistry; Synthesis

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Ramos, A. A. (2012). Obtenção e caracterização de ftalocianinas com substituintes quirais com potencial aplicação em terapia fotodinâmica. (Thesis). Universidade Federal do ABC. Retrieved from http://tede.ufabc.edu.br/tde_busca/arquivo.php?codArquivo=262

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Ramos, Aline Alves. “Obtenção e caracterização de ftalocianinas com substituintes quirais com potencial aplicação em terapia fotodinâmica.” 2012. Thesis, Universidade Federal do ABC. Accessed January 23, 2021. http://tede.ufabc.edu.br/tde_busca/arquivo.php?codArquivo=262.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Ramos, Aline Alves. “Obtenção e caracterização de ftalocianinas com substituintes quirais com potencial aplicação em terapia fotodinâmica.” 2012. Web. 23 Jan 2021.

Vancouver:

Ramos AA. Obtenção e caracterização de ftalocianinas com substituintes quirais com potencial aplicação em terapia fotodinâmica. [Internet] [Thesis]. Universidade Federal do ABC; 2012. [cited 2021 Jan 23]. Available from: http://tede.ufabc.edu.br/tde_busca/arquivo.php?codArquivo=262.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Ramos AA. Obtenção e caracterização de ftalocianinas com substituintes quirais com potencial aplicação em terapia fotodinâmica. [Thesis]. Universidade Federal do ABC; 2012. Available from: http://tede.ufabc.edu.br/tde_busca/arquivo.php?codArquivo=262

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Wollongong

21. Rineh, Ardeshir. Synthesis and biological evaluation of phenothiazinium-efflux pump inhibitor hybrids as new agents for use in antimicrobial photodynamic therapy.

Degree: PhD, 2015, University of Wollongong

URL: 030503 Organic Chemical Synthesis, 039903 Industrial Chemistry, 030499 Medicinal and Biomolecular Chemistry not elsewhere classified, 060802 Animal Cell and Molecular Biology ; https://ro.uow.edu.au/theses/4384

► Antimicrobial photodynamic therapy (aPDT) uses photosensitiser (PS) molecules in combination with visible light to generate reactive oxygen species (ROS; e.g. 1O2 and •OH) that… (more)

Subjects/Keywords: antimicrobial photodynamic therapy; efflux pump inhibitor; gram-positive S. aureus; phenothiazinium photosensitizer. methylene blue; INF55

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Rineh, A. (2015). Synthesis and biological evaluation of phenothiazinium-efflux pump inhibitor hybrids as new agents for use in antimicrobial photodynamic therapy. (Doctoral Dissertation). University of Wollongong. Retrieved from 030503 Organic Chemical Synthesis, 039903 Industrial Chemistry, 030499 Medicinal and Biomolecular Chemistry not elsewhere classified, 060802 Animal Cell and Molecular Biology ; https://ro.uow.edu.au/theses/4384

Chicago Manual of Style (16^th Edition):

Rineh, Ardeshir. “Synthesis and biological evaluation of phenothiazinium-efflux pump inhibitor hybrids as new agents for use in antimicrobial photodynamic therapy.” 2015. Doctoral Dissertation, University of Wollongong. Accessed January 23, 2021. 030503 Organic Chemical Synthesis, 039903 Industrial Chemistry, 030499 Medicinal and Biomolecular Chemistry not elsewhere classified, 060802 Animal Cell and Molecular Biology ; https://ro.uow.edu.au/theses/4384.

MLA Handbook (7^th Edition):

Rineh, Ardeshir. “Synthesis and biological evaluation of phenothiazinium-efflux pump inhibitor hybrids as new agents for use in antimicrobial photodynamic therapy.” 2015. Web. 23 Jan 2021.

Vancouver:

Rineh A. Synthesis and biological evaluation of phenothiazinium-efflux pump inhibitor hybrids as new agents for use in antimicrobial photodynamic therapy. [Internet] [Doctoral dissertation]. University of Wollongong; 2015. [cited 2021 Jan 23]. Available from: 030503 Organic Chemical Synthesis, 039903 Industrial Chemistry, 030499 Medicinal and Biomolecular Chemistry not elsewhere classified, 060802 Animal Cell and Molecular Biology ; https://ro.uow.edu.au/theses/4384.

Council of Science Editors:

Rineh A. Synthesis and biological evaluation of phenothiazinium-efflux pump inhibitor hybrids as new agents for use in antimicrobial photodynamic therapy. [Doctoral Dissertation]. University of Wollongong; 2015. Available from: 030503 Organic Chemical Synthesis, 039903 Industrial Chemistry, 030499 Medicinal and Biomolecular Chemistry not elsewhere classified, 060802 Animal Cell and Molecular Biology ; https://ro.uow.edu.au/theses/4384

Wayne State University

22. Price, Michael. A Role For Reactive Oxygen Species In Photodynamic Therapy.

Degree: PhD, Cancer Biology, 2012, Wayne State University

URL: https://digitalcommons.wayne.edu/oa_dissertations/613

► Photodynamic therapy (PDT) is based on the ability of certain photosensitizing agents to selectively localize in neoplastic cells and their vasculature. Subsequent irradiation at… (more)

▼ Photodynamic therapy (PDT) is based on the ability of certain photosensitizing agents to selectively localize in neoplastic cells and their vasculature. Subsequent irradiation at a wavelength corresponding to a photosensitizer absorbance band excites the photosensitizer molecules, leading to energy transfer reactions and fluorescence. It was initially concluded that the phototoxic effect occurred when energy from the excited state of the photosensitizer was transferred to dissolved oxygen to form singlet oxygen. This product has a very brief half-life and will cause cellular damage only in the immediate vicinity of its formation. But an excited-state photosensitizer can also interact with oxygen to form superoxide anion radical, which in turn (through the action of superoxide dismutase) is converted to hydrogen peroxide and hydroxyl radical via the Fenton reaction. These reactive oxygen species (ROS) all posses properties (i.e. lifetime, reactivity and diffusion distances) that make them at least as cytotoxic as singlet oxygen. The hypothesis examined in this dissertation was that the particular reactive oxygen species being formed might be an important determinant of photodynamic therapy efficacy. Initial studies were designed to assess specificity of several commercially available fluorogenic probes that could used for identification of ROS generated during PDT. APF (for hydroxyl radical) and DADB (for singlet oxygen) were found to be useful for cell culture studies in the context of PDT while other probes were found to have less specificity than advertised. Using an inhibitor of catalase (3-AT) and an endogenous source of catalase that localizes to peroxisomes (termed CAT_SKL) it was feasible to alter the level of hydrogen peroxide produced by PDT. Promoting persistence of hydrogen peroxide resulted in enhanced photokilling and vice versa. This indicates a role for hydrogen peroxide and its downstream product in the photokilling process. The efficacy of singlet oxygen generation vs. oxygen radicals was then compared. The photosensitizer NPe6 localizes in lysosomes and generates singlet oxygen in a high yield. The photosensitizer WST11 also localized in lysosomes, and has been reported to produce only oxygen radicals upon irradiation under aqueous conditions. This was confirmed by studies using the singlet oxygen probe DADB. The efficacy of these photosensitizers was compared under conditions where the oxygenation level was varied. In cell culture, lowering the oxygenation levels from 20% to 1% did not alter the phototoxicity of NPe6. WST11 phototoxicity was reduced in the 1% oxygen. In a cell-free system, the initial rate of formation of singlet oxygen from NPe6 was independent of the oxygen content. For both NPe6 and WST11, formation of hydroxyl was, however, highly correlated with the level of oxygenation. These data indicate important roles for several different ROS that can be formed during PDT. With… Advisors/Committee Members: David Kessel.

Subjects/Keywords: fluorescent probes; hypoxia; photodynamic therapy; photosensitizer; reactive oxygen species; Cell Biology; Oncology

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Price, M. (2012). A Role For Reactive Oxygen Species In Photodynamic Therapy. (Doctoral Dissertation). Wayne State University. Retrieved from https://digitalcommons.wayne.edu/oa_dissertations/613

Chicago Manual of Style (16^th Edition):

Price, Michael. “A Role For Reactive Oxygen Species In Photodynamic Therapy.” 2012. Doctoral Dissertation, Wayne State University. Accessed January 23, 2021. https://digitalcommons.wayne.edu/oa_dissertations/613.

MLA Handbook (7^th Edition):

Price, Michael. “A Role For Reactive Oxygen Species In Photodynamic Therapy.” 2012. Web. 23 Jan 2021.

Vancouver:

Price M. A Role For Reactive Oxygen Species In Photodynamic Therapy. [Internet] [Doctoral dissertation]. Wayne State University; 2012. [cited 2021 Jan 23]. Available from: https://digitalcommons.wayne.edu/oa_dissertations/613.

Council of Science Editors:

Price M. A Role For Reactive Oxygen Species In Photodynamic Therapy. [Doctoral Dissertation]. Wayne State University; 2012. Available from: https://digitalcommons.wayne.edu/oa_dissertations/613

23. Doychinova, Maya / Дойчинова, Мая. Dental caries prophylaxis with photosensitizers (experimental, laboratory and clinical studies) // Профилактика на зъбния кариес с фотосенсибилизатори (експериментални, лабораторни и клинични изследвания).

Degree: 2015, Medical University of Varna

URL: http://repository.mu-varna.bg/handle/nls/68

► [EN] Dental caries is a social, multietiological bacterially-mediated disease with significant consequences for the oral and the overall health of the human population. Diseases related… (more)

▼ [EN] Dental caries is a social, multietiological bacterially-mediated disease with significant consequences for the oral and the overall health of the human population. Diseases related to microbial biofilms are one of the most prevalent bacterial diseases in humans. The process of destruction of hard dental tissues due to dental caries is part of these conditions of disturbed ecological balance. The role of Mutans Streptococci (MS) and Lactobacilli, as an essential part of the biofilm on the hard dental tissues and their involvement in the emergence of carious process widely recognized. The use of antimicrobial agents and agents for chemical inhibition is a valuable addition to the methods of mechanical plaque control. However the side effects and the drawbacks of these agents prevent their long-term use. Antibiotics are effective in reducing caries activity in animals and in humans, but clinically irrelevant for the prevention. One of the innovative methods for controlling biofilm-induced diseases is the Antibacterial photodynamic therapy (APDT). Although the process of photodynamic inactivation of microorganisms existed since 1900, only in the last 25 years, the scientific community once again directs its efforts towards this method, which is an alternative to conventional antimicrobial therapy. Destruction of biofilms by APDT requires application of visible light of a specific wavelength in order to excite the sensitizing agent, and to generate cytotoxic complexes, which may cause a lethal effect on the bacteria in the biofilm. There is a need for a suitable and convenient light source that can be used to irradiate all tooth surfaces. AFDT method is still in its experimental stage and is gradually becoming a method of choice, mostly for the treatment of localized infections. Biofilm-induced diseases: periodontitis, periimplantitis, periapical infectious and dental caries are particularly suitable for the prevention and treatment AFDT as fully meet the requirements for application of the method: Controlled introduction of photosensitizer in the biofilm; easy access of light and opportunity for its topical guide; spatially limiting the impact. In this study we investigated the possibilities of an innovative approach to control cariogenic biofilm based on increasingly trending AFDT as an alternative to a conventional antibiotics and antiseptics, especially in areas where there is need and opportunity for local response. In our scientific work we investigated the possibilities of three metal phthalocyanine dyes - Zn-, Si-, Ga-metal phthalocyanin and two dyes approved for clinical use - methylene blue and toluidine blue. The product licensed for clinical use is Fotosan® (active substance toludin blue). We confirmed its efficiency on a reference laboratory strain S. mutans and on a clinically isolated strain, as well as in vivo administration limiting the mass of the biofilm, in the early stages of its structuring. We were able to compare the efficacy of APDT at various time intervals of light activation and different…

Subjects/Keywords: Caries prevention; prophylaxis; photosensitizer; Methylene Blue; FotoSun®; Antibacterial photodynamic therapy; Консервативно зъболечение / Conservative Dentistry

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Doychinova, Maya / Дойчинова, �. (2015). Dental caries prophylaxis with photosensitizers (experimental, laboratory and clinical studies) // Профилактика на зъбния кариес с фотосенсибилизатори (експериментални, лабораторни и клинични изследвания). (Thesis). Medical University of Varna. Retrieved from http://repository.mu-varna.bg/handle/nls/68

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Doychinova, Maya / Дойчинова, Мая. “Dental caries prophylaxis with photosensitizers (experimental, laboratory and clinical studies) // Профилактика на зъбния кариес с фотосенсибилизатори (експериментални, лабораторни и клинични изследвания).” 2015. Thesis, Medical University of Varna. Accessed January 23, 2021. http://repository.mu-varna.bg/handle/nls/68.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Doychinova, Maya / Дойчинова, Мая. “Dental caries prophylaxis with photosensitizers (experimental, laboratory and clinical studies) // Профилактика на зъбния кариес с фотосенсибилизатори (експериментални, лабораторни и клинични изследвания).” 2015. Web. 23 Jan 2021.

Vancouver:

Doychinova, Maya / Дойчинова �. Dental caries prophylaxis with photosensitizers (experimental, laboratory and clinical studies) // Профилактика на зъбния кариес с фотосенсибилизатори (експериментални, лабораторни и клинични изследвания). [Internet] [Thesis]. Medical University of Varna; 2015. [cited 2021 Jan 23]. Available from: http://repository.mu-varna.bg/handle/nls/68.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Doychinova, Maya / Дойчинова �. Dental caries prophylaxis with photosensitizers (experimental, laboratory and clinical studies) // Профилактика на зъбния кариес с фотосенсибилизатори (експериментални, лабораторни и клинични изследвания). [Thesis]. Medical University of Varna; 2015. Available from: http://repository.mu-varna.bg/handle/nls/68

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

24. Rigaux, Guillaume. Elaboration, caractérisation et évaluation biologique de nanoparticules biocompatibles pour la thérapie photodynamique et l’imagerie IRM : Elaboration, characterization and biological evaluation of biocompatible nanoparticles for photodynamic therapy and MRI.

Degree: Docteur es, Sciences - STS, 2015, Reims

URL: http://www.theses.fr/2015REIMS026

►

L'objectif poursuivi au cours de ce travail est l'élaboration de nanoparticules biocompatibles à visée diagnostique (IRM) et thérapeutique (PDT). Dans ce but, un protocole de… (more)

▼

L'objectif poursuivi au cours de ce travail est l'élaboration de nanoparticules biocompatibles à visée diagnostique (IRM) et thérapeutique (PDT). Dans ce but, un protocole de nanoprécipitation a été optimisé pour obtenir de façon quantitative et reproductible, des nanoparticules de PLGA de diamètre compatible avec une injection par voie parentérale. Cette formulation a été employée avec succès pour l'encapsulation d'un chélate lipophile de Gd(III), pour l'encapsulation d'un photosensibilisateur (m-THPC) et pour la co-encapsulation de ces deux substances actives. Les formulations optimales permettent d'obtenir des efficacités d'encapsulation de 7 et 46 % en chélate de gadolinium et m-THPC respectivement. La cytotoxicité et la photocytotoxicité des [email protected] ont été testées sur deux lignées cellulaires (C6 et fibroblastes) et les résultats obtenus montrent que les propriétés photocytotoxiques du m-THPC sont maintenues après l'encapsulation. L'efficacité IRM de ces nanoparticules a aussi été évaluée et les mesures NMRD et IRM à 3T montrent que l'encapsulation des chélates de gadolinium améliore leur capacité à générer un contraste en mode T1 et donc la qualité des images.

This work aimed at the synthesis of biocompatible nanoparticles for PDT and MRI applications. To reach this goal, a nanoprecipitation technique was optimized using only biocompatible starting materials. This technique allowed the preparation, in a reproducible and quantitative manner of PLGA nanoparticles, compatible with parenteral injections. This formulation was successfully applied to encapsulate a lipophilic Gd(III) chelate, a photosensitizer (m-THPC) and to co-encapsulate these two substances. Optimal formulations showed encapsulation yields of 7 and 46 % for the gadolinium chelate and m-THPC, respectively. Cytotoxicity and photocytotoxicity experiments performed for two different cell lines (C6 cells and fibroblasts) incubated with [email protected] nanoparticles showed that m-THPC photocytotoxicity was maintained after its encapsulation. MRI efficacy of [email protected] nanoparticles was also evaluated by NMRD measurements and 3T MRI images. The corresponding results indicated that gadolinium chelate encapsulation improved its tendency to generate an efficient T1 contrast and consequently, enhanced the image contrast.

Advisors/Committee Members: Chuburu, Françoise (thesis director).

Subjects/Keywords: Nanoparticules de PLGA; Nanoprécipitation; Photosensibilisateurs; Pdt; Gadolinium; Irm; PLGA nanoparticles; Nanoprecipitation; Photosensitizer; Pdt; Gadolinium; Mri

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Rigaux, G. (2015). Elaboration, caractérisation et évaluation biologique de nanoparticules biocompatibles pour la thérapie photodynamique et l’imagerie IRM : Elaboration, characterization and biological evaluation of biocompatible nanoparticles for photodynamic therapy and MRI. (Doctoral Dissertation). Reims. Retrieved from http://www.theses.fr/2015REIMS026

Chicago Manual of Style (16^th Edition):

Rigaux, Guillaume. “Elaboration, caractérisation et évaluation biologique de nanoparticules biocompatibles pour la thérapie photodynamique et l’imagerie IRM : Elaboration, characterization and biological evaluation of biocompatible nanoparticles for photodynamic therapy and MRI.” 2015. Doctoral Dissertation, Reims. Accessed January 23, 2021. http://www.theses.fr/2015REIMS026.

MLA Handbook (7^th Edition):

Rigaux, Guillaume. “Elaboration, caractérisation et évaluation biologique de nanoparticules biocompatibles pour la thérapie photodynamique et l’imagerie IRM : Elaboration, characterization and biological evaluation of biocompatible nanoparticles for photodynamic therapy and MRI.” 2015. Web. 23 Jan 2021.

Vancouver:

Rigaux G. Elaboration, caractérisation et évaluation biologique de nanoparticules biocompatibles pour la thérapie photodynamique et l’imagerie IRM : Elaboration, characterization and biological evaluation of biocompatible nanoparticles for photodynamic therapy and MRI. [Internet] [Doctoral dissertation]. Reims; 2015. [cited 2021 Jan 23]. Available from: http://www.theses.fr/2015REIMS026.

Council of Science Editors:

Rigaux G. Elaboration, caractérisation et évaluation biologique de nanoparticules biocompatibles pour la thérapie photodynamique et l’imagerie IRM : Elaboration, characterization and biological evaluation of biocompatible nanoparticles for photodynamic therapy and MRI. [Doctoral Dissertation]. Reims; 2015. Available from: http://www.theses.fr/2015REIMS026

25. Sourdon, Aude. Nano-objets tout organiques pour la thérapie photodynamique biphotonique : soft organic nanoparticles for two photon photodynamic therapy.

Degree: Docteur es, Chimie, 2013, Rennes 1

URL: http://www.theses.fr/2013REN1S193

►

La thérapie photodynamique est fondée sur l’activation sélective par la lumière de médicaments appelés photosensibilisateurs. Non toxique en l’absence d’excitation lumineuse, le photosensibilisateur est capable,… (more)

▼

La thérapie photodynamique est fondée sur l’activation sélective par la lumière de médicaments appelés photosensibilisateurs. Non toxique en l’absence d’excitation lumineuse, le photosensibilisateur est capable, une fois excité, de transférer son énergie pour former de l’oxygène singulet qui induit la mort de la cellule. L’utilisation d’une excitation biphotonique offre de nouvelles perspectives pour la thérapie photodynamique du cancer. En effet, la dépendance quadratique de l’absorption à deux photons (ADP) avec l’intensité du laser permet une très grande sélectivité spatiale, ce qui rend possible un traitement plus sélectif des tumeurs, et la lumière infra-rouge utilisée permet le traitement de tumeurs plus profondes. Dans ce travail, nous avons développé une famille de nano-objets tout organiques pour la thérapie photodynamique biphotonique. Ils présentent des sections efficaces d’ADP très élevées dans la gamme spectrale d’intérêt biologique et leur efficacité a été démontrée in vitro.

Photodynamic therapy is based on the selective activation by light of drugs called photosensitizers. Non-toxic in the absence of excitation light, the photosensitizer is able, upon excitation, to transfer energy to produce singlet oxygen, which induces cell death. Two-photon excitation offers new perspectives for photodynamic therapy of cancer. Indeed, the quadratic dependence of two-photon absorption (TPA) with the laser intensity allows high spatial selectivity, which enables a more selective treatment of tumors, and the use of infrared light allows treatment of deeper tumors. In this work, we have developed a family of fully organic nano-objects for two-photon photodynamic therapy. They exhibit very high TPA cross-sections in the biological spectral range of interest and their efficiency has been demonstrated in vitro.

Advisors/Committee Members: Blanchard-Desce, Mireille (thesis director), Mongin, Olivier (thesis director).

Subjects/Keywords: Nanophotonique; Photosensibilisant; Photothérapie dynamique; Absorption biphotonique; Nanophotonics; Photosensitizer; Photodynamic therapy; Two-photon absorption

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Sourdon, A. (2013). Nano-objets tout organiques pour la thérapie photodynamique biphotonique : soft organic nanoparticles for two photon photodynamic therapy. (Doctoral Dissertation). Rennes 1. Retrieved from http://www.theses.fr/2013REN1S193

Chicago Manual of Style (16^th Edition):

Sourdon, Aude. “Nano-objets tout organiques pour la thérapie photodynamique biphotonique : soft organic nanoparticles for two photon photodynamic therapy.” 2013. Doctoral Dissertation, Rennes 1. Accessed January 23, 2021. http://www.theses.fr/2013REN1S193.

MLA Handbook (7^th Edition):

Sourdon, Aude. “Nano-objets tout organiques pour la thérapie photodynamique biphotonique : soft organic nanoparticles for two photon photodynamic therapy.” 2013. Web. 23 Jan 2021.

Vancouver:

Sourdon A. Nano-objets tout organiques pour la thérapie photodynamique biphotonique : soft organic nanoparticles for two photon photodynamic therapy. [Internet] [Doctoral dissertation]. Rennes 1; 2013. [cited 2021 Jan 23]. Available from: http://www.theses.fr/2013REN1S193.

Council of Science Editors:

Sourdon A. Nano-objets tout organiques pour la thérapie photodynamique biphotonique : soft organic nanoparticles for two photon photodynamic therapy. [Doctoral Dissertation]. Rennes 1; 2013. Available from: http://www.theses.fr/2013REN1S193

Universiteit Utrecht

26. Shi, Yang. Polymeric micelles for drug delivery : from synthesis to in vivo studies.

Degree: 2014, Universiteit Utrecht

URL: http://dspace.library.uu.nl:8080/handle/1874/300801

► Drug delivery systems have been extensively utilized to increase water-solubility of hydrophobic chemotherapeutic drugs and target the drugs to tumors, which enhances the efficacy of… (more)

▼ Drug delivery systems have been extensively utilized to increase water-solubility of hydrophobic chemotherapeutic drugs and target the drugs to tumors, which enhances the efficacy of chemotherapy and simultaneously decreases non-specific disposition of cytostatic drugs in healthy organ/tissues, and consequently avoids or minimizes toxicity/adverse effects. Among different nano-sized drug delivery systems, polymeric micelles are one of the most successful formulations applied on anti-cancer drugs, as evidenced from the fact that several micellar formulations for chemotherapeutic drugs have entered clinical trials. Polymeric micelles are extensively utilized for this purpose for the following reasons: (1) polymeric micelles possess a hydrophobic core suitable to accommodate hydrophobic (anti-cancer) compounds; (2) the hydrophilic micellar corona can provide ‘stealth’ properties and avoid their rapid removal by the reticuloendothelial system (RES); (3) polymeric micelles have a substantially lower critical micelle concentration (CMC) and therefore a better stability compared to micelles composed of small molecule surfactants; (4) micelle-forming polymers can be chemically tailored to increase the stability of polymeric micelles by introducing physical/chemical interactions between the polymer chains; (5) stimuli-sensitive micelles can be developed which release their payloads upon a certain physical trigger or upon degradation at their site of action; (6) polymeric micelles can be decorated with targeting moieties, which may potentially improve the therapeutic response by an increased uptake of drug-loaded polymeric micelles by cancer cells. Although polymeric micelles are attractive systems for tumor-targeted drug delivery, several hurdles hamper their applicability. For example, many micellar systems suffer from low stability in the blood circulation, which causes fast elimination of drug-loaded polymeric micelles after systemic administration and severely limits the tumor targeting efficacy of the systems, which is one of the main issues that the work described in this thesis aimed to address. Furthermore, applications of polymeric micelles other than tumor-targeted drug delivery have been proposed, including polymeric micelles based imaging-guided drug delivery and photodynamic therapy. This aim of this thesis is to further advance polymeric micelles as tumor-targeted carrier systems regarding the following aspects: (1) enhance the stability of polymeric micelles and drug retention in the blood circulation by means of chemical/physical interactions between the micelle forming polymer chains; (2) investigate the possibilities for the synthesis of better defined amphiphilic polymers by controlled/living polymerization, i.e., Reversible Addition Fragmentation Chain Transfer (RAFT) polymerization; (3) validate the concept of image-guided drug delivery based on fluorescently-labeled polymeric micelles; (4) study the pharmacokinetics, biodistribution and therapeutic efficacy of stabilized polymeric micelles in tumor… Advisors/Committee Members: Hennink, Wim, van Nostrum, Rene.

Subjects/Keywords: polymeric micelles; drug delivery; tumor targeting; RAFT polymerization; micellar stability; tumor regression; imaging-guided drug delivery; photodynamic therapy; photosensitizer

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Shi, Y. (2014). Polymeric micelles for drug delivery : from synthesis to in vivo studies. (Doctoral Dissertation). Universiteit Utrecht. Retrieved from http://dspace.library.uu.nl:8080/handle/1874/300801

Chicago Manual of Style (16^th Edition):

Shi, Yang. “Polymeric micelles for drug delivery : from synthesis to in vivo studies.” 2014. Doctoral Dissertation, Universiteit Utrecht. Accessed January 23, 2021. http://dspace.library.uu.nl:8080/handle/1874/300801.

MLA Handbook (7^th Edition):

Shi, Yang. “Polymeric micelles for drug delivery : from synthesis to in vivo studies.” 2014. Web. 23 Jan 2021.

Vancouver:

Shi Y. Polymeric micelles for drug delivery : from synthesis to in vivo studies. [Internet] [Doctoral dissertation]. Universiteit Utrecht; 2014. [cited 2021 Jan 23]. Available from: http://dspace.library.uu.nl:8080/handle/1874/300801.

Council of Science Editors:

Shi Y. Polymeric micelles for drug delivery : from synthesis to in vivo studies. [Doctoral Dissertation]. Universiteit Utrecht; 2014. Available from: http://dspace.library.uu.nl:8080/handle/1874/300801

27. Andrade, Cintia Teles de. Identificação de lesões de pele por detecção fotodinâmica mediada por ácido aminolevulínico.

Degree: Mestrado, Física Aplicada, 2012, University of São Paulo

URL: http://www.teses.usp.br/teses/disponiveis/76/76132/tde-26042012-105045/ ;

►

No Brasil, o câncer de pele não-melanoma é o tipo de câncer mais comum, corresponde a cerca de 95% de todos os tipos de câncer… (more)

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No Brasil, o câncer de pele não-melanoma é o tipo de câncer mais comum, corresponde a cerca de 95% de todos os tipos de câncer de pele e 25% de todo o tipo de tumor maligno. O diagnóstico precoce permite tratar lesões logo nos primeiros estágios da doença, melhorando as condições do paciente. Assim, é de grande importância desenvolver técnicas para auxiliar o diagnóstico, como a fluorescência marcada. Ela consiste em usar substâncias fotossensíveis como biomarcadores e analisar sua resposta de fluorescência à excitação por luz. O uso do ácido aminolevulínico (ALA) é de interesse para tal, pois apresenta seletividade para formação da protoporfirina IX (PpIX) em células alteradas, substância esta que apresenta boa resposta de fluorescência à excitação por luz (região do UV-Azul). Para um diagnóstico adequado, portanto, é necessário entender melhor o comportamento da luz em meios túrbidos, como a pele. Assim, o objetivo do estudo é investigar fenômenos envolvidos com esta técnica in vitro e in vivo. Para a análise in vitro foi usado um phantom de pele contendo 2% de nanquim, 1% de Lipofundin® e 5% BRIJ-35. Um derivado de porfirinas (Photogem®) foi inserido no interior dessa solução e excitado de formas diferentes para estudar o comportamento da fluorescência, da luz de excitação e de ambas. No ensaio clínico, soluções de ALA (5% e 10%) foram aplicadas em lesões de pele malignas e potencialmente malignizáveis, e em intervalos regulares de tempo (15, 30, 45 e 60 minutos), as imagens de fluorescência foram coletadas com o protótipo de um sistema de diagnóstico por imagem de fluorescência. A diferente atenuação dos comprimentos de onda envolvidos foi quantificada in vitro. Verificou-se que o limitante do diagnóstico é a luz de excitação, sua penetração limitada em meios túrbidos impede que esta chegue à camadas profundas do tecido com uma intensidade suficiente para excitar o centro fluorescente, de modo que a fluorescência emitida possa ser detectada na superfície do tecido. Na investigação in vivo, o ALA proporcionou uma fluorescência marcada que distinguiu significantemente a pele normal da tumoral e as lesões de pele pré-malignas foram identificadas através de sua autofluorescência. A técnica de diagnóstico contribuiu também para a identificação das bordas da lesão, o que é muito importante para um tratamento eficaz.

Non melanoma skin cancer is the most common cancer lesion in Brazil. It represents about 95% of all skin cancer lesions, and 25% of tumor types. Early diagnosis allows treatment of lesions in the initial stages of the disease, improving patient´s condition. Thus, it is of great importance the development of techniques to aid diagnosis, such as marked fluorescence. This technique consists in using photosensitive substances as biomarkers and analyzing their fluorescence response to light excitation. The use of aminolevulinic acid (ALA) as a biomarker precursor is interesting because it shows selectivity for protoporphyrin IX (PpIX) formation in abnormal cells. This substance shows high fluorescence…

Advisors/Committee Members: Kurachi, Cristina.

Subjects/Keywords: Phantom de pele; Câncer de pele; Diagnosis; Diagnóstico; Fluorescence; Fluorescência; Fotossensibilizador; Photosensitizer; Skin cancer; Skin phantom

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Andrade, C. T. d. (2012). Identificação de lesões de pele por detecção fotodinâmica mediada por ácido aminolevulínico. (Masters Thesis). University of São Paulo. Retrieved from http://www.teses.usp.br/teses/disponiveis/76/76132/tde-26042012-105045/ ;

Chicago Manual of Style (16^th Edition):

Andrade, Cintia Teles de. “Identificação de lesões de pele por detecção fotodinâmica mediada por ácido aminolevulínico.” 2012. Masters Thesis, University of São Paulo. Accessed January 23, 2021. http://www.teses.usp.br/teses/disponiveis/76/76132/tde-26042012-105045/ ;.

MLA Handbook (7^th Edition):

Andrade, Cintia Teles de. “Identificação de lesões de pele por detecção fotodinâmica mediada por ácido aminolevulínico.” 2012. Web. 23 Jan 2021.

Vancouver:

Andrade CTd. Identificação de lesões de pele por detecção fotodinâmica mediada por ácido aminolevulínico. [Internet] [Masters thesis]. University of São Paulo; 2012. [cited 2021 Jan 23]. Available from: http://www.teses.usp.br/teses/disponiveis/76/76132/tde-26042012-105045/ ;.

Council of Science Editors:

Andrade CTd. Identificação de lesões de pele por detecção fotodinâmica mediada por ácido aminolevulínico. [Masters Thesis]. University of São Paulo; 2012. Available from: http://www.teses.usp.br/teses/disponiveis/76/76132/tde-26042012-105045/ ;

28. Andrade, Patrícia Freitas de. Avaliação da terapia antimicrobiana fotodinâmica na modulação das respostas imunoinflamatória e cicatricial dos tecidos periodontais. Estudo clínico, controlado e randomizado em pacientes com periodontite crônica.

Degree: PhD, Periodontia, 2010, University of São Paulo

URL: http://www.teses.usp.br/teses/disponiveis/58/58132/tde-16092010-164819/ ;

► Objetivo: O objetivo deste estudo foi analisar o efeito adjunto da terapia antimicrobiana fotodinâmica (aPDT) aos procedimentos de raspagem e alisamento radicular (RAR) na modulação… (more)

▼ Objetivo: O objetivo deste estudo foi analisar o efeito adjunto da terapia antimicrobiana fotodinâmica (aPDT) aos procedimentos de raspagem e alisamento radicular (RAR) na modulação das respostas imunoinflamatória e cicatricial dos tecidos periodontais, através de análises imunoenzimática e de expressão gênica. Material e Métodos: Foram selecionados 15 pacientes portadores de Periodontite Crônica, apresentando dentes molares inferiores bilaterais com lesões de bifurcação classe III e indicados para extração. Cada par de dentes foi dividido aleatoriamente em grupo controle (GC), no qual foi realizada a RAR, e em grupo teste (GT), no qual além da RAR, foi realizada a aPDT. Previamente à execução desta terapia inicial básica (baseline: tempo 0), amostras de fluido crevicular gengival (FCG) foram coletadas, visando à determinação dos níveis de interleucina-1β (IL-1β), metaloprotease-8 (MMP-8), osteoprotegerina/ligante do receptor de ativação do fator nuclear-κβ (OPG/RANKL) e fator de crescimento transformador-vβ(TGF-β) através do teste Elisa. Após 45 dias da realização da terapia inicial (tempo 1), coletou-se novas amostras de FCG e, em seguida, foram realizadas cirurgias a retalho associadas à RAR no GC, enquanto no GT, as cirurgias a retalho foram associadas à RAR + aPDT. Após 21 dias de pós-operatório (tempo 2), amostras de FCG foram novamente coletadas, os tecidos de granulação neoformados no interior das lesões de bifurcação classe III foram removidos e armazenados para extração do RNA total e análise através do Real-Time Polymerase Chain Reaction (Real-Time PCR) e os dentes foram devidamente extraídos. O Real-Time PCR avaliou quantitativamente os níveis de mRNAs dos seguintes genes: fator de necrose tumoral alfa (TNF-&alpha), interleucina-1β (IL-1β), interleucina-4 (IL-4), interleucina-10 (IL-10), metaloproteinase-2 (MMP-2), inibidor tecidual de metaloproteinase-2 (TIMP-2), osteoprotegerina (OPG), ligante do receptor de ativação do fator nuclear-κβ (RANKL), colágeno tipo I (COL I), fosfatase alcalina (ALP), osteopontina (OPN), osteocalcina (OCN) e sialoproteína óssea (BSP). Resultados: Teste Elisa: Foram encontradas diferenças estatisticamente significantes entre os grupos em relação à concentração de TGF-β no FCG 45 dias após a terapia inicial básica (GT = 38,35pg/µl ± 71,56pg/µl; GC = 5,92pg/µl ± 11,14pg/µl; p=0,04), bem como em relação ao volume de FCG 21 dias após a realização das cirurgias a retalho (GT = 2,34µl ± 1,81µl; GC = 3,04µl ± 2,00µl; p=0,03), a favor do GT. Em ambos os grupos, não foram encontradas diferenças estatisticamente significantes entre os diferentes tempos de avaliação em relação às quantidades e concentrações de IL-1, MMP-8, relação OPG/RANKL e TGF-β no FCG. Real-Time PCR: Quanto à análise de expressão gênica, foram encontradas diferenças estatisticamente significantes entre os grupos somente para os níveis de mRNAs da MMP-2 (GT = 3,26 ± 0,89; GC = 4,23 ± 0,97; p=0,01), relação entre TIMP-2/MMP-2 (GT = 0,91 ± 0,34; GC = 0,73… Advisors/Committee Members: Bulle, Daniela Bazan Palioto, Souza, Sergio Luis Scombatti de.

Subjects/Keywords: agentes fotosensitizadores; antimicrobial photodynamic therapy; doenças periodontais/terapia; laser; laser; periodontal diseade/therapy; photosensitizer; terapia fotodinâmica

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Andrade, P. F. d. (2010). Avaliação da terapia antimicrobiana fotodinâmica na modulação das respostas imunoinflamatória e cicatricial dos tecidos periodontais. Estudo clínico, controlado e randomizado em pacientes com periodontite crônica. (Doctoral Dissertation). University of São Paulo. Retrieved from http://www.teses.usp.br/teses/disponiveis/58/58132/tde-16092010-164819/ ;

Chicago Manual of Style (16^th Edition):

Andrade, Patrícia Freitas de. “Avaliação da terapia antimicrobiana fotodinâmica na modulação das respostas imunoinflamatória e cicatricial dos tecidos periodontais. Estudo clínico, controlado e randomizado em pacientes com periodontite crônica.” 2010. Doctoral Dissertation, University of São Paulo. Accessed January 23, 2021. http://www.teses.usp.br/teses/disponiveis/58/58132/tde-16092010-164819/ ;.

MLA Handbook (7^th Edition):

Andrade, Patrícia Freitas de. “Avaliação da terapia antimicrobiana fotodinâmica na modulação das respostas imunoinflamatória e cicatricial dos tecidos periodontais. Estudo clínico, controlado e randomizado em pacientes com periodontite crônica.” 2010. Web. 23 Jan 2021.

Vancouver:

Andrade PFd. Avaliação da terapia antimicrobiana fotodinâmica na modulação das respostas imunoinflamatória e cicatricial dos tecidos periodontais. Estudo clínico, controlado e randomizado em pacientes com periodontite crônica. [Internet] [Doctoral dissertation]. University of São Paulo; 2010. [cited 2021 Jan 23]. Available from: http://www.teses.usp.br/teses/disponiveis/58/58132/tde-16092010-164819/ ;.

Council of Science Editors:

Andrade PFd. Avaliação da terapia antimicrobiana fotodinâmica na modulação das respostas imunoinflamatória e cicatricial dos tecidos periodontais. Estudo clínico, controlado e randomizado em pacientes com periodontite crônica. [Doctoral Dissertation]. University of São Paulo; 2010. Available from: http://www.teses.usp.br/teses/disponiveis/58/58132/tde-16092010-164819/ ;

29. Li, Songying. FUNCTIONALIZED SUB-10 NM SILICA METHYLENE BLUE NANOPHOTOSENSITIZERS.

Degree: M.S., Materials Science and Engineering, Materials Science and Engineering, 2018, Cornell University

URL: http://hdl.handle.net/1813/59690

► Photodynamic therapy (PDT) presents an alternative non-invasive therapeutic modality for the treatment of cancer and other diseases. PDT relies on cytotoxic singlet oxygen that is… (more)

Subjects/Keywords: photosensitizer; silica; Materials Science; Nanoparticles; photodynamic therapy

…photosensitizer (PS) and light. Absorption of light at certain wavelength excites PS molecules… …The principle of PDT can generally be described in four steps: A photosensitizer (PS… …C’ dots encapsulating photosensitizer dyes. In order to use the well-established syntheses… …of C’ dot to incorporate the photosensitizer dye, a water-soluble and photostable… …photosensitizer is required. Here, for the photosensitizer, we choose MB2, a water-soluble Type II…

10 more images…

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Li, S. (2018). FUNCTIONALIZED SUB-10 NM SILICA METHYLENE BLUE NANOPHOTOSENSITIZERS. (Masters Thesis). Cornell University. Retrieved from http://hdl.handle.net/1813/59690

Chicago Manual of Style (16^th Edition):

Li, Songying. “FUNCTIONALIZED SUB-10 NM SILICA METHYLENE BLUE NANOPHOTOSENSITIZERS.” 2018. Masters Thesis, Cornell University. Accessed January 23, 2021. http://hdl.handle.net/1813/59690.

MLA Handbook (7^th Edition):

Li, Songying. “FUNCTIONALIZED SUB-10 NM SILICA METHYLENE BLUE NANOPHOTOSENSITIZERS.” 2018. Web. 23 Jan 2021.

Vancouver:

Li S. FUNCTIONALIZED SUB-10 NM SILICA METHYLENE BLUE NANOPHOTOSENSITIZERS. [Internet] [Masters thesis]. Cornell University; 2018. [cited 2021 Jan 23]. Available from: http://hdl.handle.net/1813/59690.

Council of Science Editors:

Li S. FUNCTIONALIZED SUB-10 NM SILICA METHYLENE BLUE NANOPHOTOSENSITIZERS. [Masters Thesis]. Cornell University; 2018. Available from: http://hdl.handle.net/1813/59690

University of Michigan

30. Savellano, Mark David. Photodynamic targeting with photosensitizer immunoconjugates.

Degree: PhD, Biomedical engineering, 2000, University of Michigan

URL: http://hdl.handle.net/2027.42/132680

► Photosensitizer immunoconjugates (PICs) offer improved photosensitizer delivery specificity and could broaden the applicability of photodynamic therapy (PDT) in the treatment of various lesions (e.g., tumors,… (more)

▼ Photosensitizer immunoconjugates (PICs) offer improved photosensitizer delivery specificity and could broaden the applicability of photodynamic therapy (PDT) in the treatment of various lesions (e.g., tumors, bacterial infections, atherosclerotic lesions). Over the past two decades, efforts to develop clinically useful PICs failed primarily due to the hydrophobic and aggregative properties of porphyrin-type photosensitizers. Another major shortcoming was the lack of a proper target antigen. The target antigen must allow delivery of sufficient amounts of photosensitizer to the lesion such that phototoxicity can be achieved using clinically practical light doses. In this thesis, several measures were taken to overcome these problems. First utilization of a novel 50% DMSO/50% aqueous two-solvent system greatly diminished the tendency of photosensitizer to aggregate and bind noncovalently to the PIC, thereby allowing for (1) efficient direct Covalent linkage of photosensitizer-activated ester to antibody lysine residues while maintaining biological activity of the antibody and (2) thorough purification of the PICs by gel filtration. Second, PEGylation of a small number of antibody lysine residues dramatically enhanced the solubility of the PICs, and an albumin-assisted exchange process permitted transfer of the PICs from 50% DMSO to purely aqueous buffer without forming large, insoluble aggregates. Third, a chimeric anti epidermal growth factor receptor (EGFR) antibody was chosen as the targeting vehicle because various tumor cells overexpress EGFR, and BPD Verteporfin (BPD) was chosen as the photosensitizer because of its exceptional phototoxic potency and nearly optimal photophysical properties. Gel electrophoresis (SDS-PAGE) of the PICs verified purity and covalent linkages. Studies using EGFR-positive, EGFR-negative, and macrophage cells demonstrated that PIC phototoxicity is very specific and predominantly mediated through binding of the PIC to EGFR. Confocal microscopy indicated that PICs undergo endocytosis and localize in lysosomes, whereas free BPD diffusely stains the cytosol and perinuclear membranes. Spectroscopic studies showed that intact PICs are less photoactive than free BPD due to static concentration quenching. Time-coursed incubations revealed that PICs lysosomally breakdown and increase in potency as the incubation period is increased. The results suggest that PICs may be useful in PDT applications requiting precise targeting, provided the targeted cells highly express and/or rapidly replenish target antigen. Advisors/Committee Members: Mooney, David (advisor), Rand, Stephen (advisor).

Subjects/Keywords: Antibody Growth Factor; Immunoconjugates; Lesions; Photodynamic Targeting; Photoimmunotherapy; Photosensitizer

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Savellano, M. D. (2000). Photodynamic targeting with photosensitizer immunoconjugates. (Doctoral Dissertation). University of Michigan. Retrieved from http://hdl.handle.net/2027.42/132680

Chicago Manual of Style (16^th Edition):

Savellano, Mark David. “Photodynamic targeting with photosensitizer immunoconjugates.” 2000. Doctoral Dissertation, University of Michigan. Accessed January 23, 2021. http://hdl.handle.net/2027.42/132680.

MLA Handbook (7^th Edition):

Savellano, Mark David. “Photodynamic targeting with photosensitizer immunoconjugates.” 2000. Web. 23 Jan 2021.

Vancouver:

Savellano MD. Photodynamic targeting with photosensitizer immunoconjugates. [Internet] [Doctoral dissertation]. University of Michigan; 2000. [cited 2021 Jan 23]. Available from: http://hdl.handle.net/2027.42/132680.

Council of Science Editors:

Savellano MD. Photodynamic targeting with photosensitizer immunoconjugates. [Doctoral Dissertation]. University of Michigan; 2000. Available from: http://hdl.handle.net/2027.42/132680

Open Access Theses and Dissertations