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1.
Oates, Richard.
Model Compounds for the Photoactivation of Glycosyl Donors.
Degree: 2008, Wake Forest University
URL: http://hdl.handle.net/10339/14910 
► Nature exhibits complex arrays of carbohydrates in many different forms. Carbohydrate chemists still face many challenges in the synthesis of these complex substrates. This project…
(more)
▼ Nature exhibits complex arrays of carbohydrates in many different forms.
Carbohydrate chemists still face many challenges in the synthesis of these complex
substrates. This project aims to offer carbohydrate chemists a novel way of activating the
anomeric position of a sugar by light to promote glycosylation. This involved
photolyzing model pyran compounds, in the presence of various photosensitizers, to
induce an intramolecular cyclization. This cyclization leads to the generation of an
oxonium intermediate that may alkylate other nucleophiles, which should be analogous to
a glycosylation reaction. It was determined that a moiety with an internal sensitizer could
photochemically exchange a tetrahydropyran at 300 nm. It was also determined that 6-
methylhex-5-en-1-ol-THP ether, 6-methylhept-5-en-2-ol-THP ether, and 3-methylbut-2-
en-1-ol-THP ether will transfer a pyran to a nucleophile in the presence of stoichiometric
tetrachloroquinone at 350 nm. THP-ethers such as 6-methylhex-5-en-1-ol-THP ether and
3-methylbut-2-en-1-ol-THP will transfer a pyran to a nucleophile in presence of catalytic
dicyanobenzene at 350 nm. α-Terpineol-THP Ether will transfer a pyran at 419 nm,
using catalytic amounts of dichloroquinone. A fast and efficient way to photochemically
install a THP group on an alcohol was also discovered. The synthesis, characterization,
and mechanistic principles of these compounds are discussed as potential candidates for
glycosyl activation.
Subjects/Keywords: photoactivation
…PHOTOACTIVATION OF GLYCOSYL DONORS
Nature exhibits complex arrays of carbohydrates in many different…
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APA (6th Edition):
Oates, R. (2008). Model Compounds for the Photoactivation of Glycosyl Donors. (Thesis). Wake Forest University. Retrieved from http://hdl.handle.net/10339/14910
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Oates, Richard. “Model Compounds for the Photoactivation of Glycosyl Donors.” 2008. Thesis, Wake Forest University. Accessed January 20, 2021.
http://hdl.handle.net/10339/14910.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Oates, Richard. “Model Compounds for the Photoactivation of Glycosyl Donors.” 2008. Web. 20 Jan 2021.
Vancouver:
Oates R. Model Compounds for the Photoactivation of Glycosyl Donors. [Internet] [Thesis]. Wake Forest University; 2008. [cited 2021 Jan 20].
Available from: http://hdl.handle.net/10339/14910.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Oates R. Model Compounds for the Photoactivation of Glycosyl Donors. [Thesis]. Wake Forest University; 2008. Available from: http://hdl.handle.net/10339/14910
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Louisiana State University
2.
Hendrix, Amanda Yoesting.
Base-caged Adenosine Triphosphate as a Model System for Photoactivatable Small Interfering RNA.
Degree: MSBAE, Engineering, 2012, Louisiana State University
URL: etd-06062012-164102
;
https://digitalcommons.lsu.edu/gradschool_theses/527
► Photocaged adenosine triphosphate (ATP) is one of the earliest examples of exerting spatial-temporal control over the activity of a substrate. The activity of ATP is…
(more)
▼ Photocaged adenosine triphosphate (ATP) is one of the earliest examples of exerting spatial-temporal control over the activity of a substrate. The activity of ATP is blocked until near-ultraviolet light exposure photocleaves the cage moiety. Caged ATP has been used for a myriad of applications including kinetic studies of ATP-dependent enzymes. Traditional caging of ATP occurs at the gamma-phosphate, which has been found to competitively inhibit several enzymatic systems. It was hypothesized that blocking access to the adenosine N6 position via cage molecule would prevent the initial enzyme-substrate binding event from occurring prior to photolysis, effectively minimizing competitive inhibition. Utilizing a convertible nucleoside analog of ATP, this work synthesized, purified, and characterized a form of caged ATP which, by attaching the cage molecule to the nucleobase, did not inhibit the enzymatic activity of luciferase in vitro. Characterization was accomplished via UV/Vis spectroscopy, high performance liquid chromatography (HPLC), nuclear magnetic resonance (NMR), and mass spectrometry (MS). Base-caged ATP was evaluated in a firefly luciferase enzymatic assay to determine the degree of bioactivity in the caged and photoactivated states and compared to the results of native (uncaged) ATP and gamma-NPE-caged ATP. Photolysis was conducted via 308 nm light from a transilluminator. Base-caged ATP did not inhibit the enzymatic system and the convertible nucleoside synthesis approach offers significant advantages over other caging techniques.
Subjects/Keywords: competitive inhibition; photoactivation; ATP; caged
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APA (6th Edition):
Hendrix, A. Y. (2012). Base-caged Adenosine Triphosphate as a Model System for Photoactivatable Small Interfering RNA. (Masters Thesis). Louisiana State University. Retrieved from etd-06062012-164102 ; https://digitalcommons.lsu.edu/gradschool_theses/527
Chicago Manual of Style (16th Edition):
Hendrix, Amanda Yoesting. “Base-caged Adenosine Triphosphate as a Model System for Photoactivatable Small Interfering RNA.” 2012. Masters Thesis, Louisiana State University. Accessed January 20, 2021.
etd-06062012-164102 ; https://digitalcommons.lsu.edu/gradschool_theses/527.
MLA Handbook (7th Edition):
Hendrix, Amanda Yoesting. “Base-caged Adenosine Triphosphate as a Model System for Photoactivatable Small Interfering RNA.” 2012. Web. 20 Jan 2021.
Vancouver:
Hendrix AY. Base-caged Adenosine Triphosphate as a Model System for Photoactivatable Small Interfering RNA. [Internet] [Masters thesis]. Louisiana State University; 2012. [cited 2021 Jan 20].
Available from: etd-06062012-164102 ; https://digitalcommons.lsu.edu/gradschool_theses/527.
Council of Science Editors:
Hendrix AY. Base-caged Adenosine Triphosphate as a Model System for Photoactivatable Small Interfering RNA. [Masters Thesis]. Louisiana State University; 2012. Available from: etd-06062012-164102 ; https://digitalcommons.lsu.edu/gradschool_theses/527
Louisiana State University
3.
Moll, Alyson Nanette.
Silver Nanoparticle Oligonucleotide Conjugate For Targeted Gene Silencing.
Degree: MSES, Engineering, 2014, Louisiana State University
URL: etd-11152014-131055
;
https://digitalcommons.lsu.edu/gradschool_theses/499
► This project explored a gene-regulated chemotherapy using a silver nanoparticle (SNP) conjugated with deoxyribozyme (DNAzyme) oligonucleotides which target a mutated gene in select cancer cells,…
(more)
▼ This project explored a gene-regulated chemotherapy using a silver nanoparticle (SNP) conjugated with deoxyribozyme (DNAzyme) oligonucleotides which target a mutated gene in select cancer cells, sensitize them to doxorubicin treatment. Light exposure to the SNP-DNAzyme conjugates disengages the oligonucleotides and permits specific cleavage of the Kirsten Rat Sarcomal Oncogene Homolog (K-RAS) mRNA. These conjugates could provide spatiotemporal specificity in killing only those photoexposed cells with the mutant gene. Synthesis, functionalization and characterization of citrate and hydroxypropyl cellulose SNP conjugates confirmed attachment and photolytic release of the thiol-modified 10-23 DNAzyme. Gel electrophoresis was used to demonstrate DNAzyme photoactivation, showing greater K-RAS RNA degradation when disengaged compared to the SNP-tethered form. DNAzyme in the tethered form was also protected from DNAse degradation compared to photolyzed DNAzyme. Characterization of the toxicity and localization of the nanoparticle drug delivery system constructed for the release of a photolabile DNA oligonucleotide was checked within several sets of cells to check for temporal and spatial control efficiency. MTS, alamar blue, and flow cytometry assays were performed to assess cell viability in several sets of cell cultures, including HEK293 and MCF-7 (wildtype K-RAS), SW480 and MDA-MB-231 (mutant K-RAS), and 3T3 (negative control) lines. Following the 5-day experimental protocol involving staggered treatment with SNP-DNAzyme, UV light, and doxorubicin, no cell group showed the intended pattern of necrosis in mutant K-RAS cells without morbidity in controls or partial treatments. Thus further evaluation of K-RAS+/- cells which respond consistently in viability assays is necessary before this strategy can be deemed of potential as a targeted therapeutic.
Subjects/Keywords: silver nanoparticles; photoactivation; deoxyribozy
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APA (6th Edition):
Moll, A. N. (2014). Silver Nanoparticle Oligonucleotide Conjugate For Targeted Gene Silencing. (Masters Thesis). Louisiana State University. Retrieved from etd-11152014-131055 ; https://digitalcommons.lsu.edu/gradschool_theses/499
Chicago Manual of Style (16th Edition):
Moll, Alyson Nanette. “Silver Nanoparticle Oligonucleotide Conjugate For Targeted Gene Silencing.” 2014. Masters Thesis, Louisiana State University. Accessed January 20, 2021.
etd-11152014-131055 ; https://digitalcommons.lsu.edu/gradschool_theses/499.
MLA Handbook (7th Edition):
Moll, Alyson Nanette. “Silver Nanoparticle Oligonucleotide Conjugate For Targeted Gene Silencing.” 2014. Web. 20 Jan 2021.
Vancouver:
Moll AN. Silver Nanoparticle Oligonucleotide Conjugate For Targeted Gene Silencing. [Internet] [Masters thesis]. Louisiana State University; 2014. [cited 2021 Jan 20].
Available from: etd-11152014-131055 ; https://digitalcommons.lsu.edu/gradschool_theses/499.
Council of Science Editors:
Moll AN. Silver Nanoparticle Oligonucleotide Conjugate For Targeted Gene Silencing. [Masters Thesis]. Louisiana State University; 2014. Available from: etd-11152014-131055 ; https://digitalcommons.lsu.edu/gradschool_theses/499
Iowa State University
4.
Peterson, Julie Ann.
Design and synthesis of photoactivated BODIPY dyes for photocaging and superresolution imaging with visible light.
Degree: 2020, Iowa State University
URL: https://lib.dr.iastate.edu/etd/17893
► The research herein describes design of BODIPY derived photoremovable protecting groups. Chapter 1 describes the design and synthesis of red-shifted BODIPY photoremovable protecting groups. The…
(more)
▼ The research herein describes design of BODIPY derived photoremovable protecting groups. Chapter 1 describes the design and synthesis of red-shifted BODIPY photoremovable protecting groups. The initial boron-fluorinated BODIPY derivatives have low quantum yields of photorelease. Boron-methylation of these red-shifted BODIPY photoremovable protecting groups effectively increases quantum yields of release. The boron methyl bond is thermally stable; however, upon irradiation it is cleaved and replaced with solvent, resulting in a chromophore with a higher quantum yield of fluorescence. Chapter 2 describes the application of this photoproduct for single molecule localization microscopy. Chapter 3 discusses the ability for the boron-methylated BODIPY derivatives to release alcohols directly from the meso position without use of a carbonate linkage. Chapter 4 describes selective photorelease of red and green BODIPY photoremovable protecting groups along with a UV-absorbing coumarin photoremovable protecting group.
Subjects/Keywords: BODIPY; Photoactivation; Photocage; Photoremovable protecting group
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APA (6th Edition):
Peterson, J. A. (2020). Design and synthesis of photoactivated BODIPY dyes for photocaging and superresolution imaging with visible light. (Thesis). Iowa State University. Retrieved from https://lib.dr.iastate.edu/etd/17893
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Peterson, Julie Ann. “Design and synthesis of photoactivated BODIPY dyes for photocaging and superresolution imaging with visible light.” 2020. Thesis, Iowa State University. Accessed January 20, 2021.
https://lib.dr.iastate.edu/etd/17893.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Peterson, Julie Ann. “Design and synthesis of photoactivated BODIPY dyes for photocaging and superresolution imaging with visible light.” 2020. Web. 20 Jan 2021.
Vancouver:
Peterson JA. Design and synthesis of photoactivated BODIPY dyes for photocaging and superresolution imaging with visible light. [Internet] [Thesis]. Iowa State University; 2020. [cited 2021 Jan 20].
Available from: https://lib.dr.iastate.edu/etd/17893.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Peterson JA. Design and synthesis of photoactivated BODIPY dyes for photocaging and superresolution imaging with visible light. [Thesis]. Iowa State University; 2020. Available from: https://lib.dr.iastate.edu/etd/17893
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
5.
Chaabouni, Slim.
Contribution à la chimie des cinnamates ortho-fonctionnalisés : Exploitation comme (1) précurseurs synthétiques d'hétérocycles, (2) antennes photoactivables de complexes d'ions lanthanides luminescents : Contribution to the chemistry of ortho-functionalized cinnamates : exploitation as (1) building blocks for heterocyclic synthesis, (2) photoactivatable sensitizers of luminescent lanthanide complexes.
Degree: Docteur es, Chimie organique, 2017, Université Toulouse III – Paul Sabatier
URL: http://www.theses.fr/2017TOU30104
► La chimie des cinnamates ortho-fonctionnalisés est particulièrement riche et variée, comme en témoigne le nombre important de méthodologies de synthèse reposant sur leur utilisation comme…
(more)
▼ La chimie des cinnamates ortho-fonctionnalisés est particulièrement riche et variée, comme en témoigne le nombre important de méthodologies de synthèse reposant sur leur utilisation comme substrats précurseurs d'hétérocycles d'intérêt biologique. En effet, suivant la nature du groupe fonctionnel et les conditions opératoires, des substrats à base cinnamique ont déjà été exploités avec succès pour la construction d'un large panel de squelettes hétérocycliques, allant de systèmes cycliques à cinq chaînons (benzofurane, indole, ...) à des cycles à six chaînons (coumarine, quinolin(on)e, ...). Dans ce contexte, ma thèse avait pour objectif principal d'apporter une contribution à cette chimie propre aux cinnamates ortho-fonctionnalisés. Le premier objectif de mon travail a porté sur le développement de nouvelles méthodes de synthèse hétérocyclique employant des cinnamates ortho-fonctionnalisés comme précurseurs. D'une part, une méthode de synthèse de coumarines et de quinolones 3-trifluorométhylées a été mise au point à partir de d'ortho-hydroxy- et d'ortho-aminocinnamates. Une étude mécanistique a également été conduite et a permis de mettre évidence la nature radicalaire de la réaction de trifluorométhylation concernée. D'autre part, nous avons évalué avec succès le potentiel synthétique d'ortho-azidocinnamates pour la construction photoinduite d'indoles et de quinoléines. Le second objectif de mon travail a concerné la conception, la synthèse et l'évaluation photophysique/photochimique de nouvelles sondes photoactivables luminescentes à base d'ions lanthanides. Dans ce cas, le motif cinnamique a été installé sur un complexe de lanthanide non luminescent afin de jouer le rôle d'antenne photoactivable. Après une première étude ayant permis de prouver le concept, toute une série de composés a été préparée afin d'établir des relations structures-propriétés, relations qui nous ont finalement permis d'optimiser la performance de cette nouvelle classe de sondes luminescentes photoactivables.
The chemistry of ortho-functionalized cinnamates is particularly rich and varied, as evidenced by the large number of methods using them as building blocks for the synthesis of biologically-relevant heterocycles. In fact, depending on the nature of the functional group and the reaction conditions, various cinnamic-based substrates have already been successfully employed for the synthesis of a wide range of heterocyclic skeletons, ranging from five-membered ring systems (benzofuran, indole, ...) to six-membered rings (coumarin, quinolin(on)e, ...). In this context, the main goal of my thesis was to make a significant contribution to the chemistry peculiar to ortho-functionalized cinnamates. The first objective of my work dealt with the development of new methods for heterocyclic synthesis using ortho-functionalized cinnamates as building blocks. On one hand, a method for the regioselective synthesis of 3-trifluoromethylated coumarins and quinolones was elaborated starting from ortho-hydroxy- and ortho-aminocinnamates. The reaction…
Advisors/Committee Members: Chassaing, Stefan (thesis director), Abid, Souhir (thesis director).
Subjects/Keywords: Cinnamates; Coumarines; Indoles; Lanthanide; Photoactivation; Luminescence; Cinnamates; Coumarins; Indoles; Lanthanide; Photoactivation; Luminescence
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APA (6th Edition):
Chaabouni, S. (2017). Contribution à la chimie des cinnamates ortho-fonctionnalisés : Exploitation comme (1) précurseurs synthétiques d'hétérocycles, (2) antennes photoactivables de complexes d'ions lanthanides luminescents : Contribution to the chemistry of ortho-functionalized cinnamates : exploitation as (1) building blocks for heterocyclic synthesis, (2) photoactivatable sensitizers of luminescent lanthanide complexes. (Doctoral Dissertation). Université Toulouse III – Paul Sabatier. Retrieved from http://www.theses.fr/2017TOU30104
Chicago Manual of Style (16th Edition):
Chaabouni, Slim. “Contribution à la chimie des cinnamates ortho-fonctionnalisés : Exploitation comme (1) précurseurs synthétiques d'hétérocycles, (2) antennes photoactivables de complexes d'ions lanthanides luminescents : Contribution to the chemistry of ortho-functionalized cinnamates : exploitation as (1) building blocks for heterocyclic synthesis, (2) photoactivatable sensitizers of luminescent lanthanide complexes.” 2017. Doctoral Dissertation, Université Toulouse III – Paul Sabatier. Accessed January 20, 2021.
http://www.theses.fr/2017TOU30104.
MLA Handbook (7th Edition):
Chaabouni, Slim. “Contribution à la chimie des cinnamates ortho-fonctionnalisés : Exploitation comme (1) précurseurs synthétiques d'hétérocycles, (2) antennes photoactivables de complexes d'ions lanthanides luminescents : Contribution to the chemistry of ortho-functionalized cinnamates : exploitation as (1) building blocks for heterocyclic synthesis, (2) photoactivatable sensitizers of luminescent lanthanide complexes.” 2017. Web. 20 Jan 2021.
Vancouver:
Chaabouni S. Contribution à la chimie des cinnamates ortho-fonctionnalisés : Exploitation comme (1) précurseurs synthétiques d'hétérocycles, (2) antennes photoactivables de complexes d'ions lanthanides luminescents : Contribution to the chemistry of ortho-functionalized cinnamates : exploitation as (1) building blocks for heterocyclic synthesis, (2) photoactivatable sensitizers of luminescent lanthanide complexes. [Internet] [Doctoral dissertation]. Université Toulouse III – Paul Sabatier; 2017. [cited 2021 Jan 20].
Available from: http://www.theses.fr/2017TOU30104.
Council of Science Editors:
Chaabouni S. Contribution à la chimie des cinnamates ortho-fonctionnalisés : Exploitation comme (1) précurseurs synthétiques d'hétérocycles, (2) antennes photoactivables de complexes d'ions lanthanides luminescents : Contribution to the chemistry of ortho-functionalized cinnamates : exploitation as (1) building blocks for heterocyclic synthesis, (2) photoactivatable sensitizers of luminescent lanthanide complexes. [Doctoral Dissertation]. Université Toulouse III – Paul Sabatier; 2017. Available from: http://www.theses.fr/2017TOU30104
6.
Gimenez, Paul.
Radiothérapie par photoactivation de nanoparticules et effet Mössbauer : Radiotherapy by photoactivation of nanoparticles and Mössbauer effect.
Degree: Docteur es, Biotechnologie, instrumentation, signal et imagerie pour la biologie, la médecine et l'environnement, 2015, Université Grenoble Alpes (ComUE)
URL: http://www.theses.fr/2015GREAS038
► Une radiothérapie efficace nécessite un dépôt de dose localisé à la tumeur, et donc un contraste entre le tissu tumoral et les tissus sains environnants.…
(more)
▼ Une radiothérapie efficace nécessite un dépôt de dose localisé à la tumeur, et donc un contraste entre le tissu tumoral et les tissus sains environnants. Une irradiation de basse énergie monochromatique au synchrotron d'une tumeur chargée en éléments lourds permet de maximiser l'interaction photoélectrique dans la tumeur et d'épargner les tissus sains, car les photoélectrons et les électrons Auger produits ont un TEL très élevé et déposent leur énergie autour des éléments lourds, augmentant fortement le dépôt de dose. Ils peuvent induire des dommages à l'ADN (cassures double brin) fortement létaux. Un autre phénomène permet également de promouvoir l'émission d'électrons Auger et d'augmenter ainsi la dose, l'effet Mössbauer. Cette interaction résonante et sans recul spécifique à certains isotopes dont le 57Fe présente une section efficace 450 fois plus importante que celle de l'effet photoélectrique. Ce travail de doctorat a évalué l'utilisation in vitro de nanoparticules de magnétite combinées à ces deux effets physiques. Les nanoparticules présentent une internalisation et une distribution dans les cellules F98 qui sont très propices à la radiosensibilisation : de grandes concentrations proches du noyau des cellules, et peu de toxicité ont été obtenues. Ceci a permis d'obtenir par photoactivation des NPFe un facteur d'augmentation de 3 ce qui est considérable. Ce travail multidisciplinaire rassemble des expériences de physique, de biologie et de chimie, pour évaluer les applications de nanoparticules de fer à la radiothérapie.
An efficient radiotherapy needs a localized dose to the tumour, which means a high contrast between tumorous and healthy tissues. A synchrotron low energy monochromatic irradiation of a tumour charged in high-Z elements allows maximizing photoelectric interactions in the tumour and spare the healthy tissues. Photoelectrons and high LET Auger electrons thus produced deposit their energy locally, enhancing radiation dose to tumor cells. Another interaction allows to enhance the dose by Auger electrons: the Mössbauer effect. This resonant and recoilless interaction specific to some isotopes like 57Fe has a cross section 450 times bigger than photoelectric effect. This thesis evaluates the in vitro use of magnetite nanoparticles combined with those 2 types of interactions. The nanoparticles evaluated present a high internalisation and a perinuclear distribution inside F98 cells. A dose-enhancement factor of 3 was obtained by photo activation of the iron Nps, this represents a huge increase. This multidisciplinary work encompasses experiments in chemistry, physics and biology in order to evaluate the applications of magnetite nanoparticles to radiotherapy.
Advisors/Committee Members: Elleaume, Hélène (thesis director), Ravanat, Jean-Luc (thesis director).
Subjects/Keywords: Nanoparticules; Gliome; Auger; Photoactivation; Synchrotron; Mössbauer; Nanoparticles; Glioma; Auger; Photoactivation; Synchrotron; Mössbauer; 570
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APA ·
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APA (6th Edition):
Gimenez, P. (2015). Radiothérapie par photoactivation de nanoparticules et effet Mössbauer : Radiotherapy by photoactivation of nanoparticles and Mössbauer effect. (Doctoral Dissertation). Université Grenoble Alpes (ComUE). Retrieved from http://www.theses.fr/2015GREAS038
Chicago Manual of Style (16th Edition):
Gimenez, Paul. “Radiothérapie par photoactivation de nanoparticules et effet Mössbauer : Radiotherapy by photoactivation of nanoparticles and Mössbauer effect.” 2015. Doctoral Dissertation, Université Grenoble Alpes (ComUE). Accessed January 20, 2021.
http://www.theses.fr/2015GREAS038.
MLA Handbook (7th Edition):
Gimenez, Paul. “Radiothérapie par photoactivation de nanoparticules et effet Mössbauer : Radiotherapy by photoactivation of nanoparticles and Mössbauer effect.” 2015. Web. 20 Jan 2021.
Vancouver:
Gimenez P. Radiothérapie par photoactivation de nanoparticules et effet Mössbauer : Radiotherapy by photoactivation of nanoparticles and Mössbauer effect. [Internet] [Doctoral dissertation]. Université Grenoble Alpes (ComUE); 2015. [cited 2021 Jan 20].
Available from: http://www.theses.fr/2015GREAS038.
Council of Science Editors:
Gimenez P. Radiothérapie par photoactivation de nanoparticules et effet Mössbauer : Radiotherapy by photoactivation of nanoparticles and Mössbauer effect. [Doctoral Dissertation]. Université Grenoble Alpes (ComUE); 2015. Available from: http://www.theses.fr/2015GREAS038
7.
Martin, Paul Ryan.
Studies on the Primary Mechanisms of (6-4) photolyase : Photoactivation and DNA Repair : Etudes sur les mécanismes primaires de la photolyase (6-4) : photoactivation et photoréparation de l'ADN.
Degree: Docteur es, Chimie physique et chimie analytique, 2014, Université Pierre et Marie Curie – Paris VI
URL: http://www.theses.fr/2014PA066375
► Ce travail concerne les mécanismes photoinduits d’une flavoprotéine appartenant à la famille des cryptochromes et photolyases (CPF) : la photolyase (6-4). En utilisant de la…
(more)
▼ Ce travail concerne les mécanismes photoinduits d’une flavoprotéine appartenant à la famille des cryptochromes et photolyases (CPF) : la photolyase (6-4). En utilisant de la lumière bleue cette protéine répare un dommage de l’ADN induit par les UV, le photoproduit (6-4). Nous avons étudié cette photoreparation ainsi qu’une autre réaction photoactivée que la photolyase utilise pour réduire son cofacteur flavine, la photoactivation. Nous avons faits nos études en utilisant la photolyase (6-4) de Xenopus laevis. Nous avons étudié la photoréduction du chromophore FADox de l’enzyme par spectroscopie d’absorption transitoire femtoseconde polarisée. Nous avons observé un transfert d’électron ultrarapide (~400 fs) après excitation du chromophore FADox. Nous avons caractérisés un résidu tryptophane comme réducteur. Nous avons cherché de distinguer entre les différents tryptophanes présents dans le site de photoactivation par des mesures d’anisotropie. Les résultats obtenus suggèrent que le mécanisme de transfert d’électron dans la photolyase n’est pas compatible avec le mécanisme supposé chez les photolyases qui consiste des transferts d’électron de long d’une chaine de trois résidus tryptophane. Grâce à des trains d’impulsions courtes, nous avons démontré que la photolyase (6-4) répare l’ADN par un mécanisme à deux photons successif. Le premier photon sert à convertir le défaut (6-4) en un intermédiaire métastable, X, qui a une durée de vie de ~2 min. Un second photon absorbé pendant cette durée de vie permet d’achever le cycle de réparation.
We studied the light-induced reactions of the (6-4) photolyase, a flavoenzyme of the cryptochrome/photolyase family that repairs the UV-induced (6-4) photodamage in DNA with the aid of blue light. We studied this photorepair reaction as well as the light-induced cofactor reduction called photoactivation that the enzyme uses to bring itself to a repair-active state in the (6-4) photolyase from Xenopus laevis. We have studied the photoactivation of the FADox cofactor of the enzyme using femtosecond polarised transient absorption spectroscopy. We observed a sub-picosecond electron transfer (~400 fs) after excitation of the FADox cofactor. We were able to characterise a tryptophan residue as the electron donor. We sought to differentiate the spectroscopically identical but differently oriented tryptophan residues within the protein’s photoactivation site by transient anisotropy measurements. Our results suggest that the photoactivation mechanism is not fully compatible with the mechanism thought to be conserved among photolyases: an electron transfer mechanism via electron hopping along a chain of three highly conserved tryptophan residues.Using series of single turnover flashes, we have found that the repair reaction proceeds by a successive two-photon mechanism. The first photon converts the (6-4) lesion into a metastable intermediate X, the lifetime of which is ~2 min. Absorption of a second photon within the lifetime of X results to the restoration of intact nucleobases. In light of…
Advisors/Committee Members: Plaza, Pascal (thesis director).
Subjects/Keywords: Défauts ADN; Photoproduit (6-4); Photolyase; Photoréparation; Photoactivation; Transfert d'électron photoinduit; Photoactivation; Repair; 547.2
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APA (6th Edition):
Martin, P. R. (2014). Studies on the Primary Mechanisms of (6-4) photolyase : Photoactivation and DNA Repair : Etudes sur les mécanismes primaires de la photolyase (6-4) : photoactivation et photoréparation de l'ADN. (Doctoral Dissertation). Université Pierre et Marie Curie – Paris VI. Retrieved from http://www.theses.fr/2014PA066375
Chicago Manual of Style (16th Edition):
Martin, Paul Ryan. “Studies on the Primary Mechanisms of (6-4) photolyase : Photoactivation and DNA Repair : Etudes sur les mécanismes primaires de la photolyase (6-4) : photoactivation et photoréparation de l'ADN.” 2014. Doctoral Dissertation, Université Pierre et Marie Curie – Paris VI. Accessed January 20, 2021.
http://www.theses.fr/2014PA066375.
MLA Handbook (7th Edition):
Martin, Paul Ryan. “Studies on the Primary Mechanisms of (6-4) photolyase : Photoactivation and DNA Repair : Etudes sur les mécanismes primaires de la photolyase (6-4) : photoactivation et photoréparation de l'ADN.” 2014. Web. 20 Jan 2021.
Vancouver:
Martin PR. Studies on the Primary Mechanisms of (6-4) photolyase : Photoactivation and DNA Repair : Etudes sur les mécanismes primaires de la photolyase (6-4) : photoactivation et photoréparation de l'ADN. [Internet] [Doctoral dissertation]. Université Pierre et Marie Curie – Paris VI; 2014. [cited 2021 Jan 20].
Available from: http://www.theses.fr/2014PA066375.
Council of Science Editors:
Martin PR. Studies on the Primary Mechanisms of (6-4) photolyase : Photoactivation and DNA Repair : Etudes sur les mécanismes primaires de la photolyase (6-4) : photoactivation et photoréparation de l'ADN. [Doctoral Dissertation]. Université Pierre et Marie Curie – Paris VI; 2014. Available from: http://www.theses.fr/2014PA066375
Université de Grenoble
8.
Taupin, Florence.
Nanoparticules et rayonnement synchrotron pour le traitement des tumeurs cérébrales : Nanoparticles and Synchrotron Light for Brain Tumors Therapy.
Degree: Docteur es, Biotechnologie, Instrumentation, Signal et imagerie pour la m´edecine, la biologie et l’environnement, 2013, Université de Grenoble
URL: http://www.theses.fr/2013GRENS021
► Le traitement des gliomes de haut grade constitue aujourd'hui encore un réel enjeu médical. Les techniques actuellement disponibles sont principalement palliatives et permettent d'augmenter la…
(more)
▼ Le traitement des gliomes de haut grade constitue aujourd'hui encore un réel enjeu médical. Les techniques actuellement disponibles sont principalement palliatives et permettent d'augmenter la survie des patients de quelques mois seulement. Une technique innovante de radiothérapie consiste à renforcer la dose déposée dans la tumeur grâce à l'injection d'atomes lourds de manière spécifique dans celle-ci au préalable d'une irradiation de photons de basse énergie (50-100 keV). Cette technique a fait l'objet d'essais précliniques et maintenant d'essais cliniques de phases I et II sur la ligne médicale du synchrotron Européen dont le rayonnement monochromatique et intense est particulièrement adapté pour l'application. L'utilisation d'un agent de contraste (AC) iodé (Z=53) injecté par voie veineuse permet d'améliorer le bénéfice de la radiothérapie mais n'est cependant pas suffisante pour l'élimination complète de la tumeur. En effet, l'accumulation passive d'atomes lourds dans la tumeur n'est pas assez importante et le caractère extracellulaire d'un AC ne maximise pas l'efficacité biologique de l'irradiation. Les nanoparticules (NPs) métalliques apparaissent comme un moyen efficace pour repousser ces limites. Dans le cadre de cette thèse, des études ont été conduites sur la lignée cellulaire de gliome F98 afin de caractériser la toxicité et l'internalisation de trois types de nanoparticules différents : nanoparticules de gadolinium (GdNPs 3 nm), d'or (AuNPs 13 nm) et de platine (PtNPs 6 nm). La survie cellulaire a également été évaluée après différentes conditions d'irradiation de photons monochromatiques en présence de ces nano-objets. La dépendance de la réponse cellulaire à l'énergie du rayonnement incident ainsi qu'à la distribution subcellulaire des NPs a permis de mettre en évidence plusieurs mécanismes mis en jeu dans ce traitement. A concentration identique, les NPs diminuent la survie cellulaire de manière plus importante qu'un AC, validant ainsi l'intérêt microdosimétrique des NPs. L'effet est préférentiel à basse énergie (keV) indiquant que la
photoactivation des atomes lourds est en partie responsable la réponse cellulaire. Par ailleurs, les GdNPs et les PtNPs se sont aussi montrées efficaces pour diminuer la survie cellulaire en combinaison à une irradiation à haute énergie (1.25 MeV) indiquant qu'un mécanisme de radiosensibilisation différent de la
photoactivation intervient également. Les études précliniques, ont montré que le recouvrement complet de la tumeur par les NPs constitue un point clé pour garantir le bénéfice thérapeutique du traitement. Dans cette optique, une méthode de tomographie à deux énergies développée au synchrotron, a été caractérisée dans le cadre de ce travail. L'étude a permis d'imager de manière quantitative et simultanée la tumeur (mise en évidence par un AC iodé) et son recouvrement par des GdNPs (injectées par voie directe) chez le rongeur porteur d'un gliome. La correspondance entre la distribution de l'AC et la tumeur a également été étudiée à l'aide de techniques d'imagerie à…
Advisors/Committee Members: Elleaume, Hélène (thesis director), Ravanat, Jean-Luc (thesis director).
Subjects/Keywords: Nanoparticules; Photoactivation; Radiosensibilisation; Tomographie à deux énergies; Tumeur cérébrale; Synchrotron; Nanoparticles; Photoactivation; Radiosensitization; Dual energy CT; Brain tumor; Synchrotron
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APA (6th Edition):
Taupin, F. (2013). Nanoparticules et rayonnement synchrotron pour le traitement des tumeurs cérébrales : Nanoparticles and Synchrotron Light for Brain Tumors Therapy. (Doctoral Dissertation). Université de Grenoble. Retrieved from http://www.theses.fr/2013GRENS021
Chicago Manual of Style (16th Edition):
Taupin, Florence. “Nanoparticules et rayonnement synchrotron pour le traitement des tumeurs cérébrales : Nanoparticles and Synchrotron Light for Brain Tumors Therapy.” 2013. Doctoral Dissertation, Université de Grenoble. Accessed January 20, 2021.
http://www.theses.fr/2013GRENS021.
MLA Handbook (7th Edition):
Taupin, Florence. “Nanoparticules et rayonnement synchrotron pour le traitement des tumeurs cérébrales : Nanoparticles and Synchrotron Light for Brain Tumors Therapy.” 2013. Web. 20 Jan 2021.
Vancouver:
Taupin F. Nanoparticules et rayonnement synchrotron pour le traitement des tumeurs cérébrales : Nanoparticles and Synchrotron Light for Brain Tumors Therapy. [Internet] [Doctoral dissertation]. Université de Grenoble; 2013. [cited 2021 Jan 20].
Available from: http://www.theses.fr/2013GRENS021.
Council of Science Editors:
Taupin F. Nanoparticules et rayonnement synchrotron pour le traitement des tumeurs cérébrales : Nanoparticles and Synchrotron Light for Brain Tumors Therapy. [Doctoral Dissertation]. Université de Grenoble; 2013. Available from: http://www.theses.fr/2013GRENS021
University of Cambridge
9.
Sun, Shuang.
Peptide/protein stapling and unstapling : introduction and photorelease of isobutylene graft.
Degree: PhD, 2019, University of Cambridge
URL: https://doi.org/10.17863/CAM.44729
;
https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.787876
► Intramolecular side-chain-to-side-chain crosslinking, also termed "stapling", is an important technology in the development of bioactive peptide-based therapeutics. Among all of the tools, S-alkylation is one…
(more)
▼ Intramolecular side-chain-to-side-chain crosslinking, also termed "stapling", is an important technology in the development of bioactive peptide-based therapeutics. Among all of the tools, S-alkylation is one of the most flexible approaches, as a wide range of bis-thiol-reactive linkers are commercially available. More importantly, this efficient and mild modification can be applied on large biomolecules by re-bridging their disulfides. At the same time, the handle introduced by the method provides further opportunities for manipulating the bioactivity of the molecule. The first part of this work describes a new peptide-macrocyclisation strategy with an isobutylene graft. The reaction is mild and proceeds rapidly and efficiently both with linear and cyclic peptide substrates. The resulting isobutylene-grafted peptides possess improved passive membrane permeability, and are stable in human plasma and in the presence of glutathione. This strategy can be applied to bioactive cyclic peptides such as somatostatin. Importantly, we found that structural preorganisation forced by the isobutylene graft leads to a significant improvement in the binding affinity of somatostatin to its receptor. Next, the same approach was optimised for re-bridging the disulfides of monoclonal antibodies. A 'one-pot' stapling strategy using isobutylene motifs was able to stabilise the interchain disulfides of antibodies by avoiding Fab exchange and disulfide shuffling. This general method was applied to an anti-HER2 Fab fragment and full-length IgGs under mild and biocompatible conditions. The binding affinity of the antibody was enhanced, relative to its native form, after stapling. The stapled structure maintained its effector functions and behaved similarly to its native form in vivo. The last part of the thesis describes a photoactivation decaging method of isobutylene-caged thiols through a UV-initiated thiol-ene reaction. The method was demonstrated with an isobutylene-caged cysteine, cyclic disulfide-peptide, and thiol-containing drug, all of which were rapidly and efficiently released under mild UV irradiation in the presence of thiol sources and a photoinitiator. Importantly, it was shown that the activity of histone deacetylase inhibitor largazole can be switched off when stapled, but selectively switched on within cancer cells when irradiated with non-phototoxic light. Further optimisation also demonstrated a potential application on isobutylene grafted protein-small molecule conjugates. Overall, the work presented here offers a new tool for producing cyclic bioactive peptides, stabilising therapeutic antibodies and photoactivation of thiol-containing drugs.
Subjects/Keywords: cyclised peptide; disulfides; isobutylene; stapling; thiol-ene; photoactivation
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APA (6th Edition):
Sun, S. (2019). Peptide/protein stapling and unstapling : introduction and photorelease of isobutylene graft. (Doctoral Dissertation). University of Cambridge. Retrieved from https://doi.org/10.17863/CAM.44729 ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.787876
Chicago Manual of Style (16th Edition):
Sun, Shuang. “Peptide/protein stapling and unstapling : introduction and photorelease of isobutylene graft.” 2019. Doctoral Dissertation, University of Cambridge. Accessed January 20, 2021.
https://doi.org/10.17863/CAM.44729 ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.787876.
MLA Handbook (7th Edition):
Sun, Shuang. “Peptide/protein stapling and unstapling : introduction and photorelease of isobutylene graft.” 2019. Web. 20 Jan 2021.
Vancouver:
Sun S. Peptide/protein stapling and unstapling : introduction and photorelease of isobutylene graft. [Internet] [Doctoral dissertation]. University of Cambridge; 2019. [cited 2021 Jan 20].
Available from: https://doi.org/10.17863/CAM.44729 ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.787876.
Council of Science Editors:
Sun S. Peptide/protein stapling and unstapling : introduction and photorelease of isobutylene graft. [Doctoral Dissertation]. University of Cambridge; 2019. Available from: https://doi.org/10.17863/CAM.44729 ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.787876
University of Edinburgh
10.
Farley, Sarah J.
Platinum complexes as potential photochemotherapeutic agents.
Degree: PhD, 2010, University of Edinburgh
URL: http://hdl.handle.net/1842/3769
► A major challenge of platinum anticancer therapy lies in overcoming the severe side-effects associated with treatment. Photoactivatable PtIV azido complexes, which are stable in the…
(more)
▼ A major challenge of platinum anticancer therapy lies in overcoming the severe side-effects associated with treatment. Photoactivatable PtIV azido complexes, which are stable in the dark and reduced to cytotoxic PtII species upon irradiation, have recently emerged as a potential site-specific treatment. This thesis is concerned with the investigation of PtII and PtIV azido complexes as potential cytotoxic and photochemotherapeutic agents. PtII azido complexes such as [Pt(en)(N3)2] were shown to bind to both 5'-guanosine monophosphate (5'-GMP) and glutathione, at a much reduced rate compared with their PtII chlorido analogues. Interestingly, and unexpectedly, these PtII azido complexes showed moderate cytotoxicity towards the A2780 cancer cell line (IC50 21–47 μM). Binding to 5'-GMP was observed to occur more rapidly upon irradiation with UVA light, although the extent of binding was low and the complexes did not demonstrate phototoxicity towards HaCaT keratinocytes. The pendant hydroxyl group of a PtII azido complex was functionalised with a fluorescent probe; conjugation to one axial hydroxyl ligand of a PtIV azido complex was also achieved. The latter conjugate showed a rapid increase in fluorescence intensity upon irradiation, resulting from loss of the axial ligands upon photoreduction. The functionalisation of quantum dots with PtII complexes was also investigated. Water soluble CdSe-ZnS quantum dots were synthesised and derivatised with an amine ligand to which platinum was bound. Conjugation of apo-transferrin to quantum dots was also achieved, with subsequent platinum binding yielding a conjugate with improved aqueous solubility and fluorescence properties. However, the conjugate was inactive towards the A2780 cancer cell line, likely due to surface modifications preventing cellular internalisation. PtII chlorido and azido conjugates with a porphyrin were synthesised and found to show differing behaviour upon irradiation with visible light; evidence of hydrogen peroxide generation from the chlorido complex was much reduced in the case of the azido complex; it is suggested this may result from quenching of reactive oxygen species by the azide anion released upon irradiation. PtII chlorido and azido complexes of highly coloured azo ligands were synthesised in an attempt to shift the wavelength of activation into the visible region. TD-DFT calculations allowed frontier orbital analysis and assignment of the transitions in the absorption spectra. Irradiation of the PtII azido complexes with UVA or broadband visible light led to their decomposition; one water-soluble complex was found to show moderate cytotoxicity and phototoxicity; in addition, its intense blue colour allowed for visual monitoring of this complex inside cells.
Subjects/Keywords: 615; platinum; anticancer; photoactivation
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APA (6th Edition):
Farley, S. J. (2010). Platinum complexes as potential photochemotherapeutic agents. (Doctoral Dissertation). University of Edinburgh. Retrieved from http://hdl.handle.net/1842/3769
Chicago Manual of Style (16th Edition):
Farley, Sarah J. “Platinum complexes as potential photochemotherapeutic agents.” 2010. Doctoral Dissertation, University of Edinburgh. Accessed January 20, 2021.
http://hdl.handle.net/1842/3769.
MLA Handbook (7th Edition):
Farley, Sarah J. “Platinum complexes as potential photochemotherapeutic agents.” 2010. Web. 20 Jan 2021.
Vancouver:
Farley SJ. Platinum complexes as potential photochemotherapeutic agents. [Internet] [Doctoral dissertation]. University of Edinburgh; 2010. [cited 2021 Jan 20].
Available from: http://hdl.handle.net/1842/3769.
Council of Science Editors:
Farley SJ. Platinum complexes as potential photochemotherapeutic agents. [Doctoral Dissertation]. University of Edinburgh; 2010. Available from: http://hdl.handle.net/1842/3769
11.
Szabo, Vivien.
Optogenetics in freely behaving mice with a fiberscope : Optogénétique chez la souris éveillée et mobile à l’aide d’un fibroscope.
Degree: Docteur es, Neurosciences, 2013, Université Paris Descartes – Paris V
URL: http://www.theses.fr/2013PA05T089
► Les techniques optogénétiques ont laissé entrevoir un potentiel exceptionnel dans l’étude des mécanismes gouvernant l’intégration de l’information dans le système nerveux. Afin d’établir les relations…
(more)
▼ Les techniques optogénétiques ont laissé entrevoir un potentiel exceptionnel dans l’étude des mécanismes gouvernant l’intégration de l’information dans le système nerveux. Afin d’établir les relations existant entre des séquences d’activité neuronale définies et le comportement, les techniques optiques doivent permettre d’appréhender des groupes de neurones avec une résolution cellulaire chez l’animal éveillé et mobile. Jusqu’alors, l’activité neuronale chez l’animal vigile non contraint n’a été contrôlée que via l’illumination en champs large. Dans ce travail, nous démontrons une résolution proche de la cellule unique pour la photoactivation, chez l’animal éveillé et mobile, à l’aide d’un fibroscope. Les motifs de photoactivation, produits par holographie, sont transmis jusqu’à la souris par un guide d’image couplé à un micro-objectif. Une imagerie de fluorescence permet de localiser les cellules d’intérêt et d’enregistrer l’activité neuronale, par épifluorescence, illumination structurée, ou encore microscopie confocale multi-point sans balayage. Le fibroscope est testé chez l’animal anesthésié et chez l’animal éveillé et mobile, dont les interneurones de la couche moléculaire du cervelet expriment les protéines ChR2-tdTomato et GCaMP. Nous avons généré des signaux calciques somatiques en ciblant les corps cellulaires avec des spots de photoactivation de 5µm de diamètre. Chez l’animal anesthésié, nous avons démontré que la photoactivation pouvait être réalisée avec une résolution latérale de 10µm et une résolution axiale de 40µm, en considérant la demi-largeur à mi-hauteur de la courbe de résolution. Nous avons montré qu’un ou plusieurs soma pouvaient être ciblés sélectivement. Chez l’animal éveillé et mobile, le champs de vue est resté stable au cours des acquisitions. Nous avons trouvé une résolution latérale pour la photoactivation égale à 10µm, démontrant une résolution de photoactivation proche de la cellule unique chez l’animal vigile non contraint.
Optogenetics has shown great potential to study the mechanisms governing information integration in the brain. To link specific spatiotemporal activity patterns and behaviours, optical methods should provide simultaneous access to a group of neurons with single cell resolution in freely behaving animals. So far, however, optogenetic control of neural activity in freely behaving rodents has been performed with widefield illumination only. Here, we demonstrate photoactivation with near-cellular resolution in freely behaving mice using a fiberscope. Photoactivation patterns, produced with computer-generated holography, were transmitted to the mouse using a fiber bundle coupled to a micro-objective. Fluorescence imaging allowed locating cells and recording neuronal activity, via either epifluorescence, structured illumination, or scanless multi-point confocal microscopy. The fiberscope was tested both in anesthetized and freely-behaving mice co-expressing ChR2-tdTomato and GCaMP proteins in cerebellar molecular layer interneurons. By targeting an interneuron soma…
Advisors/Committee Members: Emiliani, Valentina (thesis director).
Subjects/Keywords: Photoactivation; Imagerie calcique; Microscopie; Neurosciences; In vivo; Souris éveillée et mobile; Photoactivation; Calcium imaging; Microscopy; Neurosciences; In vivo; Freely behaving mice; 612.823 3
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APA ·
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APA (6th Edition):
Szabo, V. (2013). Optogenetics in freely behaving mice with a fiberscope : Optogénétique chez la souris éveillée et mobile à l’aide d’un fibroscope. (Doctoral Dissertation). Université Paris Descartes – Paris V. Retrieved from http://www.theses.fr/2013PA05T089
Chicago Manual of Style (16th Edition):
Szabo, Vivien. “Optogenetics in freely behaving mice with a fiberscope : Optogénétique chez la souris éveillée et mobile à l’aide d’un fibroscope.” 2013. Doctoral Dissertation, Université Paris Descartes – Paris V. Accessed January 20, 2021.
http://www.theses.fr/2013PA05T089.
MLA Handbook (7th Edition):
Szabo, Vivien. “Optogenetics in freely behaving mice with a fiberscope : Optogénétique chez la souris éveillée et mobile à l’aide d’un fibroscope.” 2013. Web. 20 Jan 2021.
Vancouver:
Szabo V. Optogenetics in freely behaving mice with a fiberscope : Optogénétique chez la souris éveillée et mobile à l’aide d’un fibroscope. [Internet] [Doctoral dissertation]. Université Paris Descartes – Paris V; 2013. [cited 2021 Jan 20].
Available from: http://www.theses.fr/2013PA05T089.
Council of Science Editors:
Szabo V. Optogenetics in freely behaving mice with a fiberscope : Optogénétique chez la souris éveillée et mobile à l’aide d’un fibroscope. [Doctoral Dissertation]. Université Paris Descartes – Paris V; 2013. Available from: http://www.theses.fr/2013PA05T089
Université Paris-Sud – Paris XI
12.
Sedoud, Arezki.
Transfert d'électrons dans le photosystème II : Electron transfer in photosystem II.
Degree: Docteur es, Biochimie, biophysique, 2011, Université Paris-Sud – Paris XI
URL: http://www.theses.fr/2011PA112028
► Le photosystème II (PSII) est un complexe multi-protéique qui utilise l'énergie solaire pour oxyder l'eau et réduire des quinones. Le site catalytique d'oxydation de l'eau…
(more)
▼ Le photosystème II (PSII) est un complexe multi-protéique qui utilise l'énergie solaire pour oxyder l'eau et réduire des quinones. Le site catalytique d'oxydation de l'eau est localisé coté lumen du complexe, alors, que le site de réduction comprenant deux quinones (QA et QB) et un fer non-hémique est localisé sur le coté stromal du complexe membranaire. Dans cette thèse j'ai étudié les deux cotés accepteur et donneur d'électrons du PSII.QA•- et QB•- sont couplés magnétiquement au fer non-hémique donnant de faibles signaux RPE. Le fer non-hémique possède quatre ligands histidines et un ligand (bi)carbonate échangeable. Le formate peut échanger le ligand (bi)carbonate induisant un ralentissement dans le transfert d'électrons. Ici, je décris une modification du signal RPE de QB•- Fe2+ lorsque le formate est substitué au (bi)carbonate. J'ai aussi découvert un second signal RPE dû à la présence du formate à la place du (bi)carbonate lorsque QB est doublement réduit. De plus, j'ai trouvé que les signaux RPE natifs de QA•- Fe2+ et QB•- Fe2+ possèdent une signature intense encore jamais détectée. Tous les signaux RPE rapportés dans cette thèse devraient faciliter le titrage redox de QB par RPE. J'ai aussi observé que QB•- peut oxyder le fer non-hémique à l'obscurité en anaérobie. Cette observation implique qu'au moins dans une fraction des centres, le couple QB•-/QBH2 possède un potentiel redox plus haut que supposé. La quantification du nombre de centres où cette oxydation du fer se produit par le couple QB•-/QBH2 reste à faire. La réduction du PSII par le dithionite génère un signal modifié de QA•-Fe2+, un changement structural du PSII observé par électrophorèse. Cela peut indiquer la réduction d'un pont disulfure à l'intérieur du PSII. Concernant le site d’oxydation de l'eau, j'ai étudié la première étape de l'assemblage du site catalytique (Mn4Ca), en suivant l'oxydation du Mn2+ par RPE en bande X et haut champ. J'ai mis au point des conditions expérimentales permettant le piégeage du premier intermédiaire et j'ai aussi trouvé une incohérence avec des travaux publiés dans la littérature. J'ai aussi trouvé que le dithionite pouvait réduire le site catalytique Mn4Ca, en formant des états sur-réduits qui peuvent correspondre aux intermédiaires de l'assemblage du cluster Mn4Ca.
Photosystem II (PSII) uses light energy to oxidise water and reduce quinone. The water oxidation site is a Mn4Ca cluster located on the luminal side of the membrane protein complex, while the quinone reduction site is made up of two quinones (QA and QB) and a non-heme Fe2+ located on the stromal side of the membrane protein. In this thesis I worked on both oxidation and reduction functions of the enzyme. QA•- and QB•- are magnetically couple to the Fe2+ giving weak and complex EPR signals. The distorted octahedral Fe2+ has four histidines ligands and an exchangeable (bi)carbonate ligand. Formate can displace the exchangeable (bi)carbonate ligand, slowing electron transfer out of the PSII reaction centre. Here I report the formate-modified QB•-…
Advisors/Committee Members: Ruetherford, Alfred william (thesis director).
Subjects/Keywords: Photosystème II; Rpe; Transfert d'électrons; Quinones; Semiquinones; Manganèse; Photoactivation; Formate; Bicarbonate.; Photosytem II; Epr; Electron transfer; Quinones; Semiquinones; Manganese; Photoactivation; Formate; Bicarbonate
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APA (6th Edition):
Sedoud, A. (2011). Transfert d'électrons dans le photosystème II : Electron transfer in photosystem II. (Doctoral Dissertation). Université Paris-Sud – Paris XI. Retrieved from http://www.theses.fr/2011PA112028
Chicago Manual of Style (16th Edition):
Sedoud, Arezki. “Transfert d'électrons dans le photosystème II : Electron transfer in photosystem II.” 2011. Doctoral Dissertation, Université Paris-Sud – Paris XI. Accessed January 20, 2021.
http://www.theses.fr/2011PA112028.
MLA Handbook (7th Edition):
Sedoud, Arezki. “Transfert d'électrons dans le photosystème II : Electron transfer in photosystem II.” 2011. Web. 20 Jan 2021.
Vancouver:
Sedoud A. Transfert d'électrons dans le photosystème II : Electron transfer in photosystem II. [Internet] [Doctoral dissertation]. Université Paris-Sud – Paris XI; 2011. [cited 2021 Jan 20].
Available from: http://www.theses.fr/2011PA112028.
Council of Science Editors:
Sedoud A. Transfert d'électrons dans le photosystème II : Electron transfer in photosystem II. [Doctoral Dissertation]. Université Paris-Sud – Paris XI; 2011. Available from: http://www.theses.fr/2011PA112028
13.
Jacomassi, Denis Pablo.
Estudo da fotoativação de resina composta variando o comprimento de onda com laser de argônio por meio, dos testes de microdureza, variação térmica, grau de conversão e ablação.
Degree: Mestrado, Ciência e Engenharia de Materiais, 2007, University of São Paulo
URL: http://www.teses.usp.br/teses/disponiveis/88/88131/tde-13092007-162139/
;
► O objetivo deste trabalho foi investigar a variação do comprimento de onda na fotoativação de resina composta com Laser de argônio, por meio, dos testes…
(more)
▼ O objetivo deste trabalho foi investigar a variação do comprimento de onda na fotoativação de resina composta com Laser de argônio, por meio, dos testes de microdureza Vickres, variação de temperatura durante o processo de polimerização, grau de conversão e ablação. Os experimentos de microdureza foram conduzidos utilizando o aparelho MMT-3 Hardness Tester (Buehler Lake Bluff, IlIinois USA) equipado com diamante Vickers. Para medir a temperatura foi utilizado um termistor de alta precisão conectado a multímetro onde foram medidos valores de resistência em (Ohm). Para medir o grau de conversão utilizou-se o Espectrofotômetro FTIR BOMEM, modelo MB-102, na faixa espectral de 4000 a 400 cm-1. As amostras foram ablacionadas com o laser de Er:YAG pulsado, no regime de microssegundos, após a ablação os diâmetros e profundidades foram medidos com uma lupa com aumento de 40X e um relógio comparador. As amostras foram ablacionadas com Laser Libra-S (Coherent, Pala Alto, CA, EUA), no regime de femtossegundos, e medidos os diâmetros das microcavidades por meio de uma imagem gerada pelo microscópio eletrônico de varredura MEV (DSM 960, Zeiss, Jena, Germany). As imagens foram processadas em um programa matemático, onde foram calculados os diâmetros e com isso, calculada a intensidade threshold de ablação. As amostras foram confeccionadas em resina composta do tipo microhíbrida Z-250 (3M-Espe) fotoativada com o laser de argônio (Coherent, Innova 200-20, USA). Foram utilizados os comprimentos de ondas de 476.5,488.0, 501.7 e 514 nm, com tempos de exposições de 15, 40, 60, 300, 900 segundos. Considerando os testes realizados o melhor comprimento de onda para a fotoativação foi 488.0 nm, conforme pôde ser visto nos experimentos abordados nessa dissertação.
The aims this work to investigate the variation of the wavelength in the photoactivation of composite resin with argon, by means, them tests of microhardness Vikres, variation of temperature during the polymerization process, degree of conversion and ablation. The microhardness experiments were lead using device MMT-3 Microhardness Tester (Buehler Lake Bluff, Illinois the USA) equipped with Vickers diamond. To measure the temperature rise the multimeter was used a thermister of high precision hardwired where values of resistance in (Ohm) were measured. To the degree of conversion was used Spectrophotometer FTIR BOMEM, model MB-102, in the spectral band of 4000 to 400cm-1. The samples were ablateds with the Er:YAG Laser, in the regimen of microseconds, the ablation the diameter and depth has been after measured with a magnifying glass with increase of 40X and comparing a clock. The sample were ablated with Libra-S Laser (Coherent, High Palo, CA, U.S.A.), in the regiment femtosecond, and measured the diameter of the microcavities, by means, of a image generated for the electron microscope of sweepings SEM (DSM 960, Zeiss, Jena, Germany). The images were processed in a mathematical program, where the diameters were calculated and with this, calculated the threshold intensity of…
Advisors/Committee Members: Bagnato, Vanderlei Salvador.
Subjects/Keywords: Argon laser; Compósito dental; Dental composite; Fotoativação; Laser de argônio; Photoactivation; Polimerização; Polymerization
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APA (6th Edition):
Jacomassi, D. P. (2007). Estudo da fotoativação de resina composta variando o comprimento de onda com laser de argônio por meio, dos testes de microdureza, variação térmica, grau de conversão e ablação. (Masters Thesis). University of São Paulo. Retrieved from http://www.teses.usp.br/teses/disponiveis/88/88131/tde-13092007-162139/ ;
Chicago Manual of Style (16th Edition):
Jacomassi, Denis Pablo. “Estudo da fotoativação de resina composta variando o comprimento de onda com laser de argônio por meio, dos testes de microdureza, variação térmica, grau de conversão e ablação.” 2007. Masters Thesis, University of São Paulo. Accessed January 20, 2021.
http://www.teses.usp.br/teses/disponiveis/88/88131/tde-13092007-162139/ ;.
MLA Handbook (7th Edition):
Jacomassi, Denis Pablo. “Estudo da fotoativação de resina composta variando o comprimento de onda com laser de argônio por meio, dos testes de microdureza, variação térmica, grau de conversão e ablação.” 2007. Web. 20 Jan 2021.
Vancouver:
Jacomassi DP. Estudo da fotoativação de resina composta variando o comprimento de onda com laser de argônio por meio, dos testes de microdureza, variação térmica, grau de conversão e ablação. [Internet] [Masters thesis]. University of São Paulo; 2007. [cited 2021 Jan 20].
Available from: http://www.teses.usp.br/teses/disponiveis/88/88131/tde-13092007-162139/ ;.
Council of Science Editors:
Jacomassi DP. Estudo da fotoativação de resina composta variando o comprimento de onda com laser de argônio por meio, dos testes de microdureza, variação térmica, grau de conversão e ablação. [Masters Thesis]. University of São Paulo; 2007. Available from: http://www.teses.usp.br/teses/disponiveis/88/88131/tde-13092007-162139/ ;
University of Manchester
14.
Wang, De-Yao.
The role of neuronal integrin-mediated adhesions in
sensing biochemical and mechanical properties of the extracellular
environment.
Degree: 2015, University of Manchester
URL: http://www.manchester.ac.uk/escholar/uk-ac-man-scw:259643
► In fibroblasts integrin receptors are the primary adhesion receptors and act as the “sensory organs” of cells to sense external signals. My aim was to…
(more)
▼ In fibroblasts integrin receptors are the primary
adhesion receptors and act as the “sensory organs” of cells to
sense external signals. My aim was to understand how integrins,
together with the adhesion-associated plaque proteins Talin and
Vinculin, regulate sensing of extracellular biochemical and
mechanical stimuli in neurons. A first set of experiments served to
characterise the sensing behaviour of CNS-derived primary cells and
cell lines on different extracellular matrix (ECM). Integrin
antibodies helped to characterise their adhesion receptor profiles
and showed that specific integrin family members were involved in
adhesion and axon/neurite outgrowth when plated on ECM proteins of
laminin (LN), fibronectin, and collagen. Data showed that
integrin-ECM interactions affected axon/neurite outgrowth and
pathfinding. Presentation of LN promoted axon/neurite outgrowth and
furthermore, presenting it in stripes dramatically enhanced
outgrowth speeds showing that not only ECM but how it is presented
directs growth. In a further set of experiments I established
procedures that enabled me to determine the impact of
mechanosensing in axon/neurite outgrowth. The established
methodology helped to define point contact areas at the base of
filopodia as sites of traction force exertion. Growth cones exerted
dynamic stresses of around 10-30 Pa. To examine whether and how
growth cones sense mechanical differences in their surrounding
environment axon/neurite outgrowth was tracked when cells were
plated on polyacrylamide. Data showed that axon/neurite outgrowth
lengths and speeds were significantly longer and faster,
respectively, on soft (0.4 kPa) than on stiff (8 kPa) substrates.
Interestingly, the depletion of vinculin in neurons blocked the
ability of growth cones to determine between soft and stiff
substrates. Altogether, the interdisciplinary approach in this
study helped to gain new insight into how neurons sense biochemical
and mechanical properties of the ECM.
Advisors/Committee Members: PROKOP, ANDREAS A, Prokop, Andreas, Ballestrem, Christoph.
Subjects/Keywords: Extracellular matrix; Mechanosensing; Adhesion signalling; Traction force; Integrin; Talin; Vinculin; Neuron; Photoactivation; Microcontact printing
Record Details
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❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Wang, D. (2015). The role of neuronal integrin-mediated adhesions in
sensing biochemical and mechanical properties of the extracellular
environment. (Doctoral Dissertation). University of Manchester. Retrieved from http://www.manchester.ac.uk/escholar/uk-ac-man-scw:259643
Chicago Manual of Style (16th Edition):
Wang, De-Yao. “The role of neuronal integrin-mediated adhesions in
sensing biochemical and mechanical properties of the extracellular
environment.” 2015. Doctoral Dissertation, University of Manchester. Accessed January 20, 2021.
http://www.manchester.ac.uk/escholar/uk-ac-man-scw:259643.
MLA Handbook (7th Edition):
Wang, De-Yao. “The role of neuronal integrin-mediated adhesions in
sensing biochemical and mechanical properties of the extracellular
environment.” 2015. Web. 20 Jan 2021.
Vancouver:
Wang D. The role of neuronal integrin-mediated adhesions in
sensing biochemical and mechanical properties of the extracellular
environment. [Internet] [Doctoral dissertation]. University of Manchester; 2015. [cited 2021 Jan 20].
Available from: http://www.manchester.ac.uk/escholar/uk-ac-man-scw:259643.
Council of Science Editors:
Wang D. The role of neuronal integrin-mediated adhesions in
sensing biochemical and mechanical properties of the extracellular
environment. [Doctoral Dissertation]. University of Manchester; 2015. Available from: http://www.manchester.ac.uk/escholar/uk-ac-man-scw:259643
University of Manchester
15.
Wang, De-Yao.
The role of neuronal integrin-mediated adhesions in sensing biochemical and mechanical properties of the extracellular environment.
Degree: PhD, 2015, University of Manchester
URL: https://www.research.manchester.ac.uk/portal/en/theses/the-role-of-neuronal-integrinmediated-adhesions-in-sensing-biochemical-and-mechanical-properties-of-the-extracellular-environment(0375a6be-784e-4768-9c9c-edf69b46e93d).html
;
https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.799244
► In fibroblasts integrin receptors are the primary adhesion receptors and act as the "sensory organs" of cells to sense external signals. My aim was to…
(more)
▼ In fibroblasts integrin receptors are the primary adhesion receptors and act as the "sensory organs" of cells to sense external signals. My aim was to understand how integrins, together with the adhesion-associated plaque proteins Talin and Vinculin, regulate sensing of extracellular biochemical and mechanical stimuli in neurons. A first set of experiments served to characterise the sensing behaviour of CNS-derived primary cells and cell lines on different extracellular matrix (ECM). Integrin antibodies helped to characterise their adhesion receptor profiles and showed that specific integrin family members were involved in adhesion and axon/neurite outgrowth when plated on ECM proteins of laminin (LN), fibronectin, and collagen. Data showed that integrin-ECM interactions affected axon/neurite outgrowth and pathfinding. Presentation of LN promoted axon/neurite outgrowth and furthermore, presenting it in stripes dramatically enhanced outgrowth speeds showing that not only ECM but how it is presented directs growth. In a further set of experiments I established procedures that enabled me to determine the impact of mechanosensing in axon/neurite outgrowth. The established methodology helped to define point contact areas at the base of filopodia as sites of traction force exertion. Growth cones exerted dynamic stresses of around 10-30 Pa. To examine whether and how growth cones sense mechanical differences in their surrounding environment axon/neurite outgrowth was tracked when cells were plated on polyacrylamide. Data showed that axon/neurite outgrowth lengths and speeds were significantly longer and faster, respectively, on soft (0.4 kPa) than on stiff (8 kPa) substrates. Interestingly, the depletion of vinculin in neurons blocked the ability of growth cones to determine between soft and stiff substrates. Altogether, the interdisciplinary approach in this study helped to gain new insight into how neurons sense biochemical and mechanical properties of the ECM.
Subjects/Keywords: Neuron; Vinculin; Talin; Integrin; Traction force; Mechanosensing; Extracellular matrix; Photoactivation; Adhesion signalling; Microcontact printing
Record Details
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Record Details
Similar Records
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« Share
❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Wang, D. (2015). The role of neuronal integrin-mediated adhesions in sensing biochemical and mechanical properties of the extracellular environment. (Doctoral Dissertation). University of Manchester. Retrieved from https://www.research.manchester.ac.uk/portal/en/theses/the-role-of-neuronal-integrinmediated-adhesions-in-sensing-biochemical-and-mechanical-properties-of-the-extracellular-environment(0375a6be-784e-4768-9c9c-edf69b46e93d).html ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.799244
Chicago Manual of Style (16th Edition):
Wang, De-Yao. “The role of neuronal integrin-mediated adhesions in sensing biochemical and mechanical properties of the extracellular environment.” 2015. Doctoral Dissertation, University of Manchester. Accessed January 20, 2021.
https://www.research.manchester.ac.uk/portal/en/theses/the-role-of-neuronal-integrinmediated-adhesions-in-sensing-biochemical-and-mechanical-properties-of-the-extracellular-environment(0375a6be-784e-4768-9c9c-edf69b46e93d).html ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.799244.
MLA Handbook (7th Edition):
Wang, De-Yao. “The role of neuronal integrin-mediated adhesions in sensing biochemical and mechanical properties of the extracellular environment.” 2015. Web. 20 Jan 2021.
Vancouver:
Wang D. The role of neuronal integrin-mediated adhesions in sensing biochemical and mechanical properties of the extracellular environment. [Internet] [Doctoral dissertation]. University of Manchester; 2015. [cited 2021 Jan 20].
Available from: https://www.research.manchester.ac.uk/portal/en/theses/the-role-of-neuronal-integrinmediated-adhesions-in-sensing-biochemical-and-mechanical-properties-of-the-extracellular-environment(0375a6be-784e-4768-9c9c-edf69b46e93d).html ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.799244.
Council of Science Editors:
Wang D. The role of neuronal integrin-mediated adhesions in sensing biochemical and mechanical properties of the extracellular environment. [Doctoral Dissertation]. University of Manchester; 2015. Available from: https://www.research.manchester.ac.uk/portal/en/theses/the-role-of-neuronal-integrinmediated-adhesions-in-sensing-biochemical-and-mechanical-properties-of-the-extracellular-environment(0375a6be-784e-4768-9c9c-edf69b46e93d).html ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.799244
The Ohio State University
16.
Rohrabaugh, Thomas Nelson, Jr.
The Application of Ru(II) Polypyridyl Photoinduced Ligand
Exchange from Drug Delivery to Photoactivation of Fluorescent
Dyes.
Degree: PhD, Chemistry, 2018, The Ohio State University
URL: http://rave.ohiolink.edu/etdc/view?acc_num=osu1533226287168103
► Photoinduced ligand exchange is a phenomenon that has been observed in Ru(II) polypyridyl complexes, where upon excitation with visible light, a bond between the metal…
(more)
▼ Photoinduced ligand exchange is a phenomenon that has
been observed in Ru(II) polypyridyl complexes, where upon
excitation with visible light, a bond between the metal and ligand
is broken, the ligand is released, and the resulting open
coordination site on the metal is filled by a solvent molecule. The
work herein describes the application of photoinduced ligand
exchange in compounds designed to possess dual activity against
cancer and other diseases in the form of a combination of
photochemotherapy (PCT) and photodynamic therapy (PDT). The poor
selectivity of conventional chemotherapy drugs, such as cisplatin,
lead to several negative side effects. PCT and PDT agents offer an
alternative to traditional chemotherapy with increased selectivity,
since the compound is only active in tissue that is exposed to
light. In addition, photoinduced ligand exchange can to applied to
photoactivation of fluorescent dyes, such that when the dye ligand
is tethered to the Ru metal center its emission quneched. Upon
irradaition with visible light, the dye ligand is released or
"uncaged", turning on its emission. Most of the photoactivatable
dyes reported to date feature organic caging groups that require
high energy UV light to release the caged molecule and activate its
fluorescence. This is not ideal for biologial applications since
high energy UV light can cause damage to living systems. Therefore,
Ru(II)–dye complexes, were synthesized and characterized. Before
photolysis, the Ru(II)-dye complexes were weakly emissive due to
quenching by the Ru(II) center.
Advisors/Committee Members: Turro, Claudia (Advisor).
Subjects/Keywords: Chemistry; Photoinduced ligand exchange; Ru polypyridyl complexes; Photochemotherapy; Photodynamic Therapy; Photoactivation of fluorescent dyes; Photochemistry
Record Details
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APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Rohrabaugh, Thomas Nelson, J. (2018). The Application of Ru(II) Polypyridyl Photoinduced Ligand
Exchange from Drug Delivery to Photoactivation of Fluorescent
Dyes. (Doctoral Dissertation). The Ohio State University. Retrieved from http://rave.ohiolink.edu/etdc/view?acc_num=osu1533226287168103
Chicago Manual of Style (16th Edition):
Rohrabaugh, Thomas Nelson, Jr. “The Application of Ru(II) Polypyridyl Photoinduced Ligand
Exchange from Drug Delivery to Photoactivation of Fluorescent
Dyes.” 2018. Doctoral Dissertation, The Ohio State University. Accessed January 20, 2021.
http://rave.ohiolink.edu/etdc/view?acc_num=osu1533226287168103.
MLA Handbook (7th Edition):
Rohrabaugh, Thomas Nelson, Jr. “The Application of Ru(II) Polypyridyl Photoinduced Ligand
Exchange from Drug Delivery to Photoactivation of Fluorescent
Dyes.” 2018. Web. 20 Jan 2021.
Vancouver:
Rohrabaugh, Thomas Nelson J. The Application of Ru(II) Polypyridyl Photoinduced Ligand
Exchange from Drug Delivery to Photoactivation of Fluorescent
Dyes. [Internet] [Doctoral dissertation]. The Ohio State University; 2018. [cited 2021 Jan 20].
Available from: http://rave.ohiolink.edu/etdc/view?acc_num=osu1533226287168103.
Council of Science Editors:
Rohrabaugh, Thomas Nelson J. The Application of Ru(II) Polypyridyl Photoinduced Ligand
Exchange from Drug Delivery to Photoactivation of Fluorescent
Dyes. [Doctoral Dissertation]. The Ohio State University; 2018. Available from: http://rave.ohiolink.edu/etdc/view?acc_num=osu1533226287168103
17.
Cusido, Janet.
Lighting Molecules with Light: Fluorescence Activation with Photochromic Oxazines.
Degree: PhD, Chemistry (Arts and Sciences), 2013, University of Miami
URL: https://scholarlyrepository.miami.edu/oa_dissertations/1067
► Photoactivatable fluorophores switch from a nonemissive to an emissive state upon illumination at an activating wavelength and then emit after irradiation at an exciting wavelength.…
(more)
▼ Photoactivatable fluorophores switch from a nonemissive to an emissive state upon illumination at an activating wavelength and then emit after irradiation at an exciting wavelength. The interplay of such activation and excitation events can be exploited to switch fluorescence on in a defined region of space at a given interval of time. In turn, the spatiotemporal control of fluorescence translates into the opportunity to implement imaging and spectroscopic schemes that are not possible with conventional fluorophores. Specifically, photoactivatable fluorophores permit the monitoring of dynamic processes in real time as well as the reconstruction of images with subdiffraction resolution. These promising applications can have a significant impact on the characterization of the structures and functions of biomolecular systems. As a result, strategies to implement mechanisms for fluorescence
photoactivation with synthetic fluorophores are particularly valuable. In this context, the work described in this thesis explores operating principles to activate the emission of organic chromophores with the aid of photochromic auxochromes. Specifically, I synthesized molecular dyads combining fluorescent and photochromic components within their covalent skeleton. These photoswitchable compounds all have an oxazine as their photochromic component and differ in the nature of their fluorescent component. In particular, I incorporated arene, borondipyrromethene (BODIPY), coumarin, cyanine, and oligothiophene fluorophores in these dyads and characterized their photochemical and photophysical properties with a combination of steady-state and time-resolved spectroscopic measurements. The compound integrating a coumarin-oxazine fluorophore turned out to be the dyad with the most spectroscopic signature for the acquisition of fluorescence images with subdifraction resolution. As a result, I developed also hydrophilic derivatives of this compound as well as a supramolecular strategy to impose hydrophilic character on it. The latter protocol is based on the encapsulation of the photoactivatable compound within the hydrophobic interior of polymer nanoparticles. Indeed, in collaborative studies, we demonstrated that these nanoscaled assemblies can be resolved in space even when they are separated by subdifraction distances on the basis of fluorescence
photoactivation.
Advisors/Committee Members: Françisco M. Raymo, Norito Takenaka, James D. Baker, James N. Wilson.
Subjects/Keywords: Photoactivatable fluorophores; oxazines; photochromism, fluorescence photoactivation, diffraction
…3
1.3. Mechanisms and Structural Designs for Irreversible Fluorescence Photoactivation… …5
1.4. Mechanisms and Structural Designs for Reversible Fluorescence Photoactivation… …the photoactivation of
fluorescence. Interest in such photoactivatable fluorophores was… …fluorescence photoactivation permits the monitoring of
dynamic processes in real time13–24 as well as… …fluorescence photoactivation with
fluorescent proteins and synthetic dyes together with their…
Record Details
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Share »
Record Details
Similar Records
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« Share
❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Cusido, J. (2013). Lighting Molecules with Light: Fluorescence Activation with Photochromic Oxazines. (Doctoral Dissertation). University of Miami. Retrieved from https://scholarlyrepository.miami.edu/oa_dissertations/1067
Chicago Manual of Style (16th Edition):
Cusido, Janet. “Lighting Molecules with Light: Fluorescence Activation with Photochromic Oxazines.” 2013. Doctoral Dissertation, University of Miami. Accessed January 20, 2021.
https://scholarlyrepository.miami.edu/oa_dissertations/1067.
MLA Handbook (7th Edition):
Cusido, Janet. “Lighting Molecules with Light: Fluorescence Activation with Photochromic Oxazines.” 2013. Web. 20 Jan 2021.
Vancouver:
Cusido J. Lighting Molecules with Light: Fluorescence Activation with Photochromic Oxazines. [Internet] [Doctoral dissertation]. University of Miami; 2013. [cited 2021 Jan 20].
Available from: https://scholarlyrepository.miami.edu/oa_dissertations/1067.
Council of Science Editors:
Cusido J. Lighting Molecules with Light: Fluorescence Activation with Photochromic Oxazines. [Doctoral Dissertation]. University of Miami; 2013. Available from: https://scholarlyrepository.miami.edu/oa_dissertations/1067
18.
ZHENG XIANG.
DEVELOPMENT OF UPCONVERSION LUMINESCENT NANOPARTICLES FOR BIOMOLECULE PHOTOACTIVATION.
Degree: 2019, National University of Singapore
URL: https://scholarbank.nus.edu.sg/handle/10635/162749
Subjects/Keywords: upconversion luminescence; lanthanide; nanomaterials; nanophotonic; photoactivation; biomedical
Record Details
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❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
XIANG, Z. (2019). DEVELOPMENT OF UPCONVERSION LUMINESCENT NANOPARTICLES FOR BIOMOLECULE PHOTOACTIVATION. (Thesis). National University of Singapore. Retrieved from https://scholarbank.nus.edu.sg/handle/10635/162749
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
XIANG, ZHENG. “DEVELOPMENT OF UPCONVERSION LUMINESCENT NANOPARTICLES FOR BIOMOLECULE PHOTOACTIVATION.” 2019. Thesis, National University of Singapore. Accessed January 20, 2021.
https://scholarbank.nus.edu.sg/handle/10635/162749.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
XIANG, ZHENG. “DEVELOPMENT OF UPCONVERSION LUMINESCENT NANOPARTICLES FOR BIOMOLECULE PHOTOACTIVATION.” 2019. Web. 20 Jan 2021.
Vancouver:
XIANG Z. DEVELOPMENT OF UPCONVERSION LUMINESCENT NANOPARTICLES FOR BIOMOLECULE PHOTOACTIVATION. [Internet] [Thesis]. National University of Singapore; 2019. [cited 2021 Jan 20].
Available from: https://scholarbank.nus.edu.sg/handle/10635/162749.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
XIANG Z. DEVELOPMENT OF UPCONVERSION LUMINESCENT NANOPARTICLES FOR BIOMOLECULE PHOTOACTIVATION. [Thesis]. National University of Singapore; 2019. Available from: https://scholarbank.nus.edu.sg/handle/10635/162749
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Louisiana State University
19.
Forman, Julianne.
Photobiological and thermal effects of UVA light on cell culture.
Degree: MSBAE, Engineering, 2005, Louisiana State University
URL: etd-11162005-100643
;
https://digitalcommons.lsu.edu/gradschool_theses/3505
► The objective of this study is to characterize the photobiological and thermal effects of UVA light on cell cultures. While near-ultraviolet light has been widely…
(more)
▼ The objective of this study is to characterize the photobiological and thermal effects of UVA light on cell cultures. While near-ultraviolet light has been widely used to photoactivate fluorophores and caged compounds in cells, little is known of the long-term biological effects of this light. UVA (320-400nm) photoactivating light has been used for studying fast kinetic processes and is now being employed in higher doses to target longer duration phenomena (e.g. gene expression and silencing). Photoexposure experiments using 365nm light will examine how longer duration and increased UV doses affect photoactivation cell studies. Apoptotic and cellular injury assays have been used to determine the nature and threshold of this light-induced effect. UVA light sources of low and high intensity have been used for light doses up to 23.85 J/cm2. HeLa cells exposed to staurosporine, high intensity UVA, and equivalent UVB served as positive UVA-induced and UVB-induced apoptotic controls. Cells were stained with annexin V-Cy5 and propidium iodide for apoptosis analysis with a conventional flow cytometer. Cell cultures at lower densities had higher percentages of apoptotic and dead cells, and were also more susceptible to UVA damage than more dense cell seedings. The dose to induce apoptosis and death in 50% of the cells (dose1/2) was determined for two different commercially available UVA light sources: 7.6 J/cm2 for the GreenSpot photocuring system and 2.52 J/cm2 for the BlakRay lamp. No significant cellular responses were found for doses below 1.6 J/cm2 from the GreenSpot light source. A temperature control and measurement system was used to determine direct heating from the UVA sources and also the effect that cooling culture dishes has on minimizing cell damage. Photoduration was also found to be significant in determining UVA photoactivation doses. Together, these results show that many of these parameters are important for optimizing photobiological studies.
Subjects/Keywords: apoptosis; HeLa; photoactivation; UVA; annexin V; thermal effects
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APA (6th Edition):
Forman, J. (2005). Photobiological and thermal effects of UVA light on cell culture. (Masters Thesis). Louisiana State University. Retrieved from etd-11162005-100643 ; https://digitalcommons.lsu.edu/gradschool_theses/3505
Chicago Manual of Style (16th Edition):
Forman, Julianne. “Photobiological and thermal effects of UVA light on cell culture.” 2005. Masters Thesis, Louisiana State University. Accessed January 20, 2021.
etd-11162005-100643 ; https://digitalcommons.lsu.edu/gradschool_theses/3505.
MLA Handbook (7th Edition):
Forman, Julianne. “Photobiological and thermal effects of UVA light on cell culture.” 2005. Web. 20 Jan 2021.
Vancouver:
Forman J. Photobiological and thermal effects of UVA light on cell culture. [Internet] [Masters thesis]. Louisiana State University; 2005. [cited 2021 Jan 20].
Available from: etd-11162005-100643 ; https://digitalcommons.lsu.edu/gradschool_theses/3505.
Council of Science Editors:
Forman J. Photobiological and thermal effects of UVA light on cell culture. [Masters Thesis]. Louisiana State University; 2005. Available from: etd-11162005-100643 ; https://digitalcommons.lsu.edu/gradschool_theses/3505
20.
Garcia, Mikael.
Rôle du couplage N-cadhérine/actine dans les mécanismes de motilité et de différentiation synaptique dans les neurones : Mechanical coupling between N-cadherin and actin in motility mechanisms and in synaptic differentiation in neurons.
Degree: Docteur es, Sciences, technologie, santé. Neurosciences, 2013, Université de Bordeaux Segalen
URL: http://www.theses.fr/2013BOR22055
► Les protéines d’adhésions homophiles N-cadhérine jouent un rôle majeur dans le développement du cerveau, notamment en agissant sur la croissance et la plasticité synaptique. Au…
(more)
▼ Les protéines d’adhésions homophiles N-cadhérine jouent un rôle majeur dans le développement du cerveau, notamment en agissant sur la croissance et la plasticité synaptique. Au cours de ma thèse, j’ai étudié le rôle de la N-cadhérine dans ces deux processus en utilisant des neurones issus de cultures primaires déposés sur des substrats micropatternés. Ces substrats sont recouverts de N-cadhérine purifiée afin d’induire des adhésions N-cadhérines sélectives au niveau de micro-motifs régulièrement espacés. Mes deux premières études sont basées sur le modèle d’embrayage moléculaire, décrivant le processus par lequel la motilité du cytosquelette d’actine se couple aux adhésions au niveau de la membrane cellulaire afin de générer des forces de traction aux zones de contact avec le substrat, permettant ainsi l’avancée cellulaire (Giannone et al., 2009). Plusieurs études ont mis en avant l’existence d’un tel modèle (Mitchison et Kirschner, 1988 ; Suter et Forscher, 1998), cependant le mécanisme exact permettant d’expliquer ce couplage mécanique de l’actine aux protéines d’adhésions reste mal connu. Via des techniques de pinces optiques, des travaux précédemment menés dans l’équipe ont prouvé l’existence d’un couplage entre le flux d’actine et les adhésions N-cadhérine permettant la migration du cône de croissance (Bard et al., 2008). Cette technique n’a cependant pas permis la visualisation directe de l’engagement d’un tel mécanisme. Nous avons donc couplé l’utilisation des substrats micro-patternés à la microscopie haute résolution sptPALM/TIRF afin de visualiser directement la dynamique des protéines impliquées dans l’embrayage moléculaire. Dans le premier article, j’ai montré pour la première fois l’existence d’interactions transitoires entre le flux d’actine et les adhésions N-cadhérines au niveau du cône de croissance, reflétant un embrayage glissant à l’échelle de la molécule unique (Garcia et al., en préparation). Dans le second article, en travaillant sur des neurones plus matures, nous avons pu montrer l’engagement d’un embrayage moléculaire trans-synaptique entre adhésions N-cadhérines et flux d’actine permettant la stabilisation du filopode dendritique et ainsi sa transition en épine mature (Chazeau/Garcia et al., en préparation). J’ai également participé à une troisième étude dans laquelle j’ai observé l’effet des substrats micropatternés recouverts de N-cadhérine, sur la synaptogenèse. J’ai ainsi pu prouver que la N-cadhérine déposée sur les micro-motifs, stimule la croissance dendritique et axonale et joue un rôle prépondérant dans la maturation morphologique des neurones. Cependant, la N-cadhérine est incapable d’induire la formation de synapses contrairement aux protéines d’adhésion neurexine/neuroligine ou SynCam (Czöndör et al., 2013).
The homophilic adhesion molecule N-cadherin plays major roles in brain development, notably affecting axon outgrowth and synaptic plasticity. During my PhD work, I addressed the role of N-cadherin in these two processes, using primary neurons cultured on micro-patterned…
Advisors/Committee Members: Thoumine, Olivier (thesis director).
Subjects/Keywords: Substrats micro-patternés; Actine; N-cadhérine; Photoactivation Localization Microscopy (PALM); Motilité; Migration du cône de croissance; Filopode dendritique; Épine dendritique; Micro-patterned substrates; Actin; N-cadherin; Photoactivation Localization Microscopy (PALM); Motility; Growth cone migration; Dendritic filopodia; Dendritic spine
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APA (6th Edition):
Garcia, M. (2013). Rôle du couplage N-cadhérine/actine dans les mécanismes de motilité et de différentiation synaptique dans les neurones : Mechanical coupling between N-cadherin and actin in motility mechanisms and in synaptic differentiation in neurons. (Doctoral Dissertation). Université de Bordeaux Segalen. Retrieved from http://www.theses.fr/2013BOR22055
Chicago Manual of Style (16th Edition):
Garcia, Mikael. “Rôle du couplage N-cadhérine/actine dans les mécanismes de motilité et de différentiation synaptique dans les neurones : Mechanical coupling between N-cadherin and actin in motility mechanisms and in synaptic differentiation in neurons.” 2013. Doctoral Dissertation, Université de Bordeaux Segalen. Accessed January 20, 2021.
http://www.theses.fr/2013BOR22055.
MLA Handbook (7th Edition):
Garcia, Mikael. “Rôle du couplage N-cadhérine/actine dans les mécanismes de motilité et de différentiation synaptique dans les neurones : Mechanical coupling between N-cadherin and actin in motility mechanisms and in synaptic differentiation in neurons.” 2013. Web. 20 Jan 2021.
Vancouver:
Garcia M. Rôle du couplage N-cadhérine/actine dans les mécanismes de motilité et de différentiation synaptique dans les neurones : Mechanical coupling between N-cadherin and actin in motility mechanisms and in synaptic differentiation in neurons. [Internet] [Doctoral dissertation]. Université de Bordeaux Segalen; 2013. [cited 2021 Jan 20].
Available from: http://www.theses.fr/2013BOR22055.
Council of Science Editors:
Garcia M. Rôle du couplage N-cadhérine/actine dans les mécanismes de motilité et de différentiation synaptique dans les neurones : Mechanical coupling between N-cadherin and actin in motility mechanisms and in synaptic differentiation in neurons. [Doctoral Dissertation]. Université de Bordeaux Segalen; 2013. Available from: http://www.theses.fr/2013BOR22055
21.
Sun, Shuang.
Peptide/Protein Stapling and Unstapling: Introduction and Photorelease of Isobutylene Graft.
Degree: PhD, 2020, University of Cambridge
URL: https://www.repository.cam.ac.uk/handle/1810/297675
► Intramolecular side-chain-to-side-chain crosslinking, also termed “stapling”, is an important technology in the development of bioactive peptide-based therapeutics. Among all of the tools, S-alkylation is one…
(more)
▼ Intramolecular side-chain-to-side-chain crosslinking, also termed “stapling”, is an important technology in the development of bioactive peptide-based therapeutics. Among all of the tools, S-alkylation is one of the most flexible approaches, as a wide range of bis-thiol-reactive linkers are commercially available. More importantly, this efficient and mild modification can be applied on large biomolecules by re-bridging their disulfides. At the same time, the handle introduced by the method provides further opportunities for manipulating the bioactivity of the molecule.
The first part of this work describes a new peptide-macrocyclisation strategy with an isobutylene graft. The reaction is mild and proceeds rapidly and efficiently both with linear and cyclic peptide substrates. The resulting isobutylene-grafted peptides possess improved passive membrane permeability, and are stable in human plasma and in the presence of glutathione. This strategy can be applied to bioactive cyclic peptides such as somatostatin. Importantly, we found that structural preorganisation forced by the isobutylene graft leads to a significant improvement in the binding affinity of somatostatin to its receptor.
Next, the same approach was optimised for re-bridging the disulfides of monoclonal antibodies. A ‘one-pot’ stapling strategy using isobutylene motifs was able to stabilise the interchain disulfides of antibodies by avoiding Fab exchange and disulfide shuffling. This general method was applied to an anti-HER2 Fab fragment and full-length IgGs under mild and biocompatible conditions. The binding affinity of the antibody was enhanced, relative to its native form, after stapling. The stapled structure maintained its effector functions and behaved similarly to its native form in vivo.
The last part of the thesis describes a photoactivation decaging method of isobutylene-caged thiols through a UV-initiated thiol-ene reaction. The method was demonstrated with an isobutylene-caged cysteine, cyclic disulfide-peptide, and thiol-containing drug, all of which were rapidly and efficiently released under mild UV irradiation in the presence of thiol sources and a photoinitiator. Importantly, it was shown that the activity of histone deacetylase inhibitor largazole can be switched off when stapled, but selectively switched on within cancer cells when irradiated with non-phototoxic light. Further optimisation also demonstrated a potential application on isobutylene grafted protein-small molecule conjugates.
Overall, the work presented here offers a new tool for producing cyclic bioactive peptides, stabilising therapeutic antibodies and photoactivation of thiol-containing drugs.
Subjects/Keywords: cyclised peptide; disulfides; isobutylene; stapling; thiol-ene; photoactivation
…Chapter 3
2. Radical‐mediated thiol‐ene strategy for photoactivation of thiol‐containing drugs… …91
CHAPTER 4 PHOTOACTIVATION OF THIOL-CONTAINING DRUGS VIA THIOL-ENE
STRATEGY… …154
5.4. PHOTOACTIVATION OF THIOL-CONTAINING DRUGS VIA THIOL-ENE STRATEGY ........... 155… …177
5.4.9. Photoactivation of stapled largazole in HCT-116 cells… …99
FIGURE 4.3 THE PHOTOACTIVATION OF ISOBUTYLENE-GRAFTED LARGAZOLE THIOL. A) THIOL…
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APA ·
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APA (6th Edition):
Sun, S. (2020). Peptide/Protein Stapling and Unstapling: Introduction and Photorelease of Isobutylene Graft. (Doctoral Dissertation). University of Cambridge. Retrieved from https://www.repository.cam.ac.uk/handle/1810/297675
Chicago Manual of Style (16th Edition):
Sun, Shuang. “Peptide/Protein Stapling and Unstapling: Introduction and Photorelease of Isobutylene Graft.” 2020. Doctoral Dissertation, University of Cambridge. Accessed January 20, 2021.
https://www.repository.cam.ac.uk/handle/1810/297675.
MLA Handbook (7th Edition):
Sun, Shuang. “Peptide/Protein Stapling and Unstapling: Introduction and Photorelease of Isobutylene Graft.” 2020. Web. 20 Jan 2021.
Vancouver:
Sun S. Peptide/Protein Stapling and Unstapling: Introduction and Photorelease of Isobutylene Graft. [Internet] [Doctoral dissertation]. University of Cambridge; 2020. [cited 2021 Jan 20].
Available from: https://www.repository.cam.ac.uk/handle/1810/297675.
Council of Science Editors:
Sun S. Peptide/Protein Stapling and Unstapling: Introduction and Photorelease of Isobutylene Graft. [Doctoral Dissertation]. University of Cambridge; 2020. Available from: https://www.repository.cam.ac.uk/handle/1810/297675
22.
Gundugola, Aditya Swaroop V.
Synthesis,
photochemistry and DNA photocleavage of compounds containing
tetrazolethione scaffolds.
Degree: PhD, Department of
Chemistry, 2011, Kansas State University
URL: http://hdl.handle.net/2097/12022
► This dissertation focused on the synthesis of 1-(2-ethynylphenyl)-4-phenyl tetrazole-5-thione derivatives 1 and evaluating their potential as a new class of photoactivated DNA cleaving prodrugs. We…
(more)
▼ This dissertation focused on the synthesis of
1-(2-ethynylphenyl)-4-phenyl tetrazole-5-thione derivatives 1 and
evaluating their potential as a new class of photoactivated DNA
cleaving prodrugs. We hypothesized that light activation of 1 would
cause the decomposition of the tetrazolethione ring system to
enyne-carbodiimide 2 with simultaneous loss of dinitrogen and
sulfur via 1,3-triplet biradicals 1′, which would be spontaneously
followed by Schmittel cyclization to indoloquinolines 3 via
benzofulvene type biradicals 2′. The biradicals 1′ and 2′ would
have the potential to cause DNA cleavage by abstracting hydrogens
from its sugar phosphate backbone, analogous to the mechanism of
action of naturally occurring enediyne antitumor antibiotics. Note
that our proposed prodrugs contained a 1,4-diaryl tetrazolethione
functionality and a direct synthetic route for their construction
was lacking in the literature despite their wide spread
applications. Therefore, our initial efforts were directed towards
developing a general strategy to obtain these ring systems. We
employed a highly versatile and efficient copper mediated
N-arylation to first obtain a series of 1,4-diaryl tetrazol-5-ones
which were thionated with Lawesson’s reagent to afford the
corresponding 1,4-diaryl tetrazole-5-thiones in moderate yields.
Specifically, the synthesis of 1 involved Sonogashira coupling of
the obtained 1-(2-bromophenyl)-4-phenyl-1H-tetrazole-5(4H)-thione
with the appropriate ethynyl compounds (Chapter 2). Since the
tetrazole ring is an important structural component in many
biologically and medicinally relevant compounds, we were interested
in evaluating the anticancer activity of these compounds in the
absence of photochemical activation. The moderate IC50 values
against leukemia and breast cancer cell lines showed that the
anticancer activity of these compounds prior to photoirradiation
was minimal (Chapter 3). Independent studies have shown that the
photodecomposition of tetrazolethiones gives carbodiimides via
biradicals, and photocyclization of enyne-carbodiimide forms
indoloquinoline also via biradicals. However, it was not known
whether these two photoreactions could happen sequentially in one
pot with one light source from a substrate like 1, generating
biradicals 1′ and 2′ which could later be employed for DNA
photocleavage as hypothesized. Therefere, we photolysed 1 in
acetonitrile, and our results show clean formation of a mixture of
enyne-carbodiimides and indoloquinolines via biradicals 1′ and 2′
(Chapter 4). Finally, we investigated DNA photocleavage by 1 at 350
nm and our results showed significant DNA cleavage in
concentrations as low as 100 μM (Chapter 5).
Advisors/Committee Members: Sundeep RayatSundeep Rayat.
Subjects/Keywords: Ethynyl;
Tetrazolethiones; Novel
chemotherapeutic drugs;
Chemotherapy;
Photoactivation;
Biradicals; Chemistry (0485)
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APA (6th Edition):
Gundugola, A. S. V. (2011). Synthesis,
photochemistry and DNA photocleavage of compounds containing
tetrazolethione scaffolds. (Doctoral Dissertation). Kansas State University. Retrieved from http://hdl.handle.net/2097/12022
Chicago Manual of Style (16th Edition):
Gundugola, Aditya Swaroop V. “Synthesis,
photochemistry and DNA photocleavage of compounds containing
tetrazolethione scaffolds.” 2011. Doctoral Dissertation, Kansas State University. Accessed January 20, 2021.
http://hdl.handle.net/2097/12022.
MLA Handbook (7th Edition):
Gundugola, Aditya Swaroop V. “Synthesis,
photochemistry and DNA photocleavage of compounds containing
tetrazolethione scaffolds.” 2011. Web. 20 Jan 2021.
Vancouver:
Gundugola ASV. Synthesis,
photochemistry and DNA photocleavage of compounds containing
tetrazolethione scaffolds. [Internet] [Doctoral dissertation]. Kansas State University; 2011. [cited 2021 Jan 20].
Available from: http://hdl.handle.net/2097/12022.
Council of Science Editors:
Gundugola ASV. Synthesis,
photochemistry and DNA photocleavage of compounds containing
tetrazolethione scaffolds. [Doctoral Dissertation]. Kansas State University; 2011. Available from: http://hdl.handle.net/2097/12022
Université Paris-Sud – Paris XI
23.
Delorme, Rachel.
Radiothérapie par Photoactivation de Nanoparticules : modélisation à l'Échelle Sub-Micrométrique et Comparaison Expérimentale : Photo-activation Therapy with Nanoparticles : modeling at a Sub-Micrometer Level and Experimental Comparison.
Degree: Docteur es, Physique médicale, 2013, Université Paris-Sud – Paris XI
URL: http://www.theses.fr/2013PA112028
► Une approche thérapeutique innovante utilisant l'adjonction d'éléments de numéro atomique élevé à une radiothérapie de basse énergie semble offrir une voie prometteuse pour le traitement…
(more)
▼ Une approche thérapeutique innovante utilisant l'adjonction d'éléments de numéro atomique élevé à une radiothérapie de basse énergie semble offrir une voie prometteuse pour le traitement des tumeurs cérébrales résistantes. Une telle technique est notamment développée sur la ligne médicale de l'ESRF (European Synchrotron Radiation Facility) utilisant un rayonnement monochromatique allant de 25 à 90 keV [Adam 2003, Adam 2006]. Des résultats encourageants ont été obtenus en traitant des souris cancéreuses après injection de nanoparticules d'or (AuNP) [Hainfeld 2004]. Cependant, les processus physiques et l'impact biologique issus de la photoactivation de nanoparticules sont encore aujourd'hui mal compris et ne peuvent être expliqués par des calculs de doses macroscopiques [Cho 2005, Zhang 2009]. Le but de ce travail est d'évaluer par simulation Monte Carlo l'augmentation locale de dose en présence de nanoparticules ainsi que les caractéristiques des électrons secondaires produits. Dans un premier temps, des simulations ont été réalisées en utilisant une géométrie cellulaire, de manière à comparer les données simulées aux expérimentations menées à l'ESRF. Des tests de clonogénicité ont été réalisés pour mesurer le taux de radiosensibilité des cellules pour une irradiation de 4 Gy (SER4Gy) en présence de gadolinium, pour différentes énergies d'irradiation (25 keV à 1250 keV). Ces études, expérimentales et numériques, ont permis l'évaluation de l'influence de la localisation du gadolinium au sein de la cellule et la forme de ce dernier (nanoparticules ou agent de contraste). D'autre part, une étude comparative a été menée pour caractériser le comportement d'une nanoparticule sous irradiation à une échelle nanométrique, en fonction de l'énergie de faisceau, du rayon de la nanoparticule et de l'élément lourd (or et gadolinium).
An innovative approach using X-ray interactions with heavy elements seems to open a promising way of treatment for resistant cancers, such as high-grade gliomas. Such a technique is developed at the medical beam line of ESRF using monochromatic X-rays in the 25-90 keV range for the treatment of brain tumors [Adam 2003, Adam 2006]. The use of gold nanoparticles (AuNP) to treat mice bearing subcutaneous tumors led to encouraging results [Hainfeld 2004]. However, the physical processes and biological impact of the photon activation of nanoparticles are not yet well understood. The experimental results cannot be explained by macroscopic dose calculations [Cho 2005, Zhang 2009]. The aim of this work was to evaluate, at the sub-cellular level, the dose enhancement in presence of nanoparticles and the properties of the secondary electrons production using Monte Carlo simulations. In a first step, simulations were performed using cell geometry, in order to compare the simulated data to the experiments realized on the ID17 beamline of ESRF. Clonogenic assays have been performed on F98 cells to measure the “Sensitizer Enhancement Ratio” for an irradiation of 4 Gy (SER4Gy) in the presence of gadolinium, for…
Advisors/Committee Members: Champion, Christophe (thesis director).
Subjects/Keywords: Radiothérapie; Monte Carlo; Simulation; Modélisation; Nanoparticules; Photoactivation; Radiosensibilité; Radiobiologie; Électrons; RX; Basse énergie; Radiotherapy; Monte Carlo; Simulation; Modeling; Nanoparticles; Photon activation; Radiosensitization; Radiobiology; Electrons; X-ray; Low energy
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APA ·
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APA (6th Edition):
Delorme, R. (2013). Radiothérapie par Photoactivation de Nanoparticules : modélisation à l'Échelle Sub-Micrométrique et Comparaison Expérimentale : Photo-activation Therapy with Nanoparticles : modeling at a Sub-Micrometer Level and Experimental Comparison. (Doctoral Dissertation). Université Paris-Sud – Paris XI. Retrieved from http://www.theses.fr/2013PA112028
Chicago Manual of Style (16th Edition):
Delorme, Rachel. “Radiothérapie par Photoactivation de Nanoparticules : modélisation à l'Échelle Sub-Micrométrique et Comparaison Expérimentale : Photo-activation Therapy with Nanoparticles : modeling at a Sub-Micrometer Level and Experimental Comparison.” 2013. Doctoral Dissertation, Université Paris-Sud – Paris XI. Accessed January 20, 2021.
http://www.theses.fr/2013PA112028.
MLA Handbook (7th Edition):
Delorme, Rachel. “Radiothérapie par Photoactivation de Nanoparticules : modélisation à l'Échelle Sub-Micrométrique et Comparaison Expérimentale : Photo-activation Therapy with Nanoparticles : modeling at a Sub-Micrometer Level and Experimental Comparison.” 2013. Web. 20 Jan 2021.
Vancouver:
Delorme R. Radiothérapie par Photoactivation de Nanoparticules : modélisation à l'Échelle Sub-Micrométrique et Comparaison Expérimentale : Photo-activation Therapy with Nanoparticles : modeling at a Sub-Micrometer Level and Experimental Comparison. [Internet] [Doctoral dissertation]. Université Paris-Sud – Paris XI; 2013. [cited 2021 Jan 20].
Available from: http://www.theses.fr/2013PA112028.
Council of Science Editors:
Delorme R. Radiothérapie par Photoactivation de Nanoparticules : modélisation à l'Échelle Sub-Micrométrique et Comparaison Expérimentale : Photo-activation Therapy with Nanoparticles : modeling at a Sub-Micrometer Level and Experimental Comparison. [Doctoral Dissertation]. Université Paris-Sud – Paris XI; 2013. Available from: http://www.theses.fr/2013PA112028
24.
Calderon, Abram.
Acute perturbation and activity measurement of Rho GTPases in living cells.
Degree: 2014, Technische Universität Dortmund
URL: http://dx.doi.org/10.17877/DE290R-15472
Subjects/Keywords: Cytoskeleton; Actin; Rho GTPase; Rac1; Cdc42; Crosstalk; Chemically induced dimerization; CID; Optogenetics; Photoactivation; Total internal reflection fluorescence; TIRF; RhoA; 570; 540
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APA ·
Chicago ·
MLA ·
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APA (6th Edition):
Calderon, A. (2014). Acute perturbation and activity measurement of Rho GTPases in living cells. (Doctoral Dissertation). Technische Universität Dortmund. Retrieved from http://dx.doi.org/10.17877/DE290R-15472
Chicago Manual of Style (16th Edition):
Calderon, Abram. “Acute perturbation and activity measurement of Rho GTPases in living cells.” 2014. Doctoral Dissertation, Technische Universität Dortmund. Accessed January 20, 2021.
http://dx.doi.org/10.17877/DE290R-15472.
MLA Handbook (7th Edition):
Calderon, Abram. “Acute perturbation and activity measurement of Rho GTPases in living cells.” 2014. Web. 20 Jan 2021.
Vancouver:
Calderon A. Acute perturbation and activity measurement of Rho GTPases in living cells. [Internet] [Doctoral dissertation]. Technische Universität Dortmund; 2014. [cited 2021 Jan 20].
Available from: http://dx.doi.org/10.17877/DE290R-15472.
Council of Science Editors:
Calderon A. Acute perturbation and activity measurement of Rho GTPases in living cells. [Doctoral Dissertation]. Technische Universität Dortmund; 2014. Available from: http://dx.doi.org/10.17877/DE290R-15472
University of Otago
25.
Nield-Patterson, Andrea.
The Effect of Photoactivation on the Mechanism of Action of tDodSNO
.
Degree: University of Otago
URL: http://hdl.handle.net/10523/5842
► Since the discovery and identification of nitric oxide (NO) in vivo more than 30 years ago, a wealth of knowledge has been collected regarding its…
(more)
▼ Since the discovery and identification of nitric oxide (NO) in vivo more than 30 years ago, a wealth of knowledge has been collected regarding its biological action. More recently, NO has been shown to be a valuable chemotherapeutic, however its application clinically has been hindered by its short half-life and toxic systemic effects. The development of NO donors has provided an avenue by which NO may be administered without unwanted side-effects, with the S-nitrosothiol class of donors showing particular promise in this regard.
The recent development of a novel photoactivated NO donor, tert-Dodecane S-nitrosothiol (tDodSNO), has further improved upon selectively controlling NO generation. With the application of light, the decomposition of tDodSNO may be controlled, making the drug relatively inert in its absence, providing a mechanism by which NO release may be modulated. tDodSNO has shown some promise as a cytotoxic agent in previous studies; however, its combination with other well established chemotherapeutics may show more potential. Despite this, little is still known about the immediate cellular action of tDodSNO following catalysis of the pro-drug to liberate NO. The present study aimed to investigate the mechanism of action of the novel photoactivated NO donor drug tDodSNO in order to establish therapeutic uses in combination with other well established chemotherapeutics. To assess this, a model of cancer was established in the MDA-MB-231 cell line and the outcomes of tDodSNO treatment were determined.
At concentrations greater than 50 μM, tDodSNO was shown to increase cellular oxidation in a
photoactivation dependent manner by up to 20.6± 0.9%. This increase in oxidation initiated cellular lipid peroxidation in a concentration-dependent manner; however this effect was not modulated by light exposure, indicating it occurred by an independent mechanism to cellular oxidation. Further, nitration and S-nitrosylation of proteins was observed at the highest 100 μM concentration, while thiol oxidation was not altered. The tumour suppressor protein, p53 was found to be accumulated 24 hours post-treatment with a 1.37 ± 0.9 fold-increase from control with 100 µM photoactivated tDodSNO, indicating cellular stress and potential DNA damage. Some of the cellular changes observed were further enhanced by irradiation of tDodSNO, which induced rapid decomposition to release NO. These findings implicate a wide range of cellular effects of tDodSNO and provided evidence for the use of tDodSNO in combination with a number of other chemotherapeutics to produce synergistic effects.
Advisors/Committee Members: Giles, Gregory (advisor).
Subjects/Keywords: Nitric Oxide Donor;
NO;
Cancer;
tDodSNO;
Oxidative Stress;
Reactive Oxygen Species;
Photoactivation;
Drug Mechanism;
Cell Signalling;
Redox
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APA (6th Edition):
Nield-Patterson, A. (n.d.). The Effect of Photoactivation on the Mechanism of Action of tDodSNO
. (Masters Thesis). University of Otago. Retrieved from http://hdl.handle.net/10523/5842
Note: this citation may be lacking information needed for this citation format:
No year of publication.
Chicago Manual of Style (16th Edition):
Nield-Patterson, Andrea. “The Effect of Photoactivation on the Mechanism of Action of tDodSNO
.” Masters Thesis, University of Otago. Accessed January 20, 2021.
http://hdl.handle.net/10523/5842.
Note: this citation may be lacking information needed for this citation format:
No year of publication.
MLA Handbook (7th Edition):
Nield-Patterson, Andrea. “The Effect of Photoactivation on the Mechanism of Action of tDodSNO
.” Web. 20 Jan 2021.
Note: this citation may be lacking information needed for this citation format:
No year of publication.
Vancouver:
Nield-Patterson A. The Effect of Photoactivation on the Mechanism of Action of tDodSNO
. [Internet] [Masters thesis]. University of Otago; [cited 2021 Jan 20].
Available from: http://hdl.handle.net/10523/5842.
Note: this citation may be lacking information needed for this citation format:
No year of publication.
Council of Science Editors:
Nield-Patterson A. The Effect of Photoactivation on the Mechanism of Action of tDodSNO
. [Masters Thesis]. University of Otago; Available from: http://hdl.handle.net/10523/5842
Note: this citation may be lacking information needed for this citation format:
No year of publication.
Freie Universität Berlin
26.
Barra Cabello, Marcos Patricio.
Disassemblierung und Assemblierung des Mangankomplexes im Photosystem II.
Degree: 2008, Freie Universität Berlin
URL: https://refubium.fu-berlin.de/handle/fub188/10717
► Licht ist essentiell sowohl als Energieressource für die photosynthesische Wasseroxidation als auch als Stimulus für die Aktivierung des Sauerstoffbildungssystems. Der Wasseroxidationskomplex des Photosystems II (PSII)…
(more)
▼ Licht ist essentiell sowohl als Energieressource für die photosynthesische
Wasseroxidation als auch als Stimulus für die Aktivierung des
Sauerstoffbildungssystems. Der Wasseroxidationskomplex des Photosystems II
(PSII) auf der Lumenseite der Thylakoidmembran im Chloroplasten spaltet Wasser
in molekularen Sauerstoff, Protonen und Elektronen. Der Mn4Ca-Komplex des
aktiven Zentrums kann vier oxidierende Redoxäquivalente akkumulieren, die
simultan durch die Oxidation zweier Wassermoleküle neutralisiert werden. Der
genaue Aufbau des Mn4Ca- Komplexes und seine Assoziierung mit den PSII
Polypeptiden werden noch kontrovers diskutiert. Die vorliegende Arbeit
beinhaltet die Ergebnisse experimenteller Untersuchungen zur Disassemblierung
und zur Assemblierung des Mangankomplexes (Mn Komplex) im PS II. Die
Kombination vielfältiger biochemischer und biophysikalischer Methoden
ermöglichte detaillierte Untersuchungen der intermediären Stadien beider
Prozesse. Durch die Anwendung einer Temperaturerhöhung (Temperatursprung auf
47°) wurde es möglich, die relative langsame schrittweise Zerlegung des Mn
Komplexes im PS II zu studieren, wobei verschiedene Intermediatzustände
auftreten. Zunächst wurden die Inaktivierung und der schrittweise Abbau des
Mn-Komplexes durch Erwärmung auf 47°C für ansteigende Zeitintervalle
untersucht. Die Wärmebehandlung wurde gewählt, um den normalerweise langsamen
Abbauprozess des Mn Komplexes zu beschleunigen und klar vom Abbauprozessen der
PSII-Proteine abzugrenzen. Durch die kombinierte Anwendung von Polarographie,
Röntgenabsorptionsspektroskopie (XAS) an der Mn K-Kante, Paragmanetisches
Elektronen resonanz (EPR), Atomabsorptionsspektroskopie (AAS) und
Rekombinations-Chlorophyll-Fluoreszenztechniken konnte gezeigt werden, dass
beim wärmeinduzierten Abbau mindestens drei Schritte ablaufen. (1) Der
schnellste Schritt ereignet sich innerhalb von fünf Minuten und ist an die
Freisetzung des extrinsischen 18 kDa Proteins des PSII gekoppelt. Die
vorliegenden Ergebnisse legen nahe, dass gleichzeitig die Affinität des
essentiellen Ca2+ Ions am Mn Komplex reduziert wird und zu der beobachteten
Inaktivierung der Sauerstoffbildung führt. (2) In einem zweiten Schritt,
werden innerhalb von fünfzehn Minuten zwei Mn Ionen zu MnII reduziert und aus
ihrer Bindung gelöst: der verbleibende binukleare Komplex beinhaltet zwei über
eine di-µ-oxo Brücke verbundene MnIII Ionen. (3) Der dritte Schritt erfolgt
erst nach Stunden und führt zur Reduktion und Freisetzung der verbleibenden
beiden Mn Ionen als MnII, wobei sich der apo-PSII mit einer leeren Mn/Ca
Bindungsstelle bildet. Der zweite Teil dieser Arbeit beinhaltet die Ergebnisse
zur Assemblierung des Mangankomplexes. Der Aufbau erfolgt im Licht durch die
sogenannte Photoaktivierung. Ausgehend von den durch Temperatursprung
erhaltenen Intermediaten wurde die Wiederherstellung der
Sauerstoffbildungsaktivität nach Photoaktivierung durch
Sauerstoffpolarographie und Messungen der verzögerten Fluoreszenz beobachtet.
Der Oxidationszustand und strukturelle Eigenschaften der…
Advisors/Committee Members: n (gender), Prof. Dr. Holger Dau (firstReferee), Prof. Dr. Wolfram Saenger (furtherReferee).
Subjects/Keywords: Photosynthesis; Photosystem II; Manganese complex; photoactivation; oxygen evolution; 500 Naturwissenschaften und Mathematik::540 Chemie::540 Chemie und zugeordnete Wissenschaften
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APA ·
Chicago ·
MLA ·
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CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Barra Cabello, M. P. (2008). Disassemblierung und Assemblierung des Mangankomplexes im Photosystem II. (Thesis). Freie Universität Berlin. Retrieved from https://refubium.fu-berlin.de/handle/fub188/10717
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Barra Cabello, Marcos Patricio. “Disassemblierung und Assemblierung des Mangankomplexes im Photosystem II.” 2008. Thesis, Freie Universität Berlin. Accessed January 20, 2021.
https://refubium.fu-berlin.de/handle/fub188/10717.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Barra Cabello, Marcos Patricio. “Disassemblierung und Assemblierung des Mangankomplexes im Photosystem II.” 2008. Web. 20 Jan 2021.
Vancouver:
Barra Cabello MP. Disassemblierung und Assemblierung des Mangankomplexes im Photosystem II. [Internet] [Thesis]. Freie Universität Berlin; 2008. [cited 2021 Jan 20].
Available from: https://refubium.fu-berlin.de/handle/fub188/10717.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Barra Cabello MP. Disassemblierung und Assemblierung des Mangankomplexes im Photosystem II. [Thesis]. Freie Universität Berlin; 2008. Available from: https://refubium.fu-berlin.de/handle/fub188/10717
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
27.
Souffrin, Agathe.
Conception, synthèse et évaluation pharmacologique de maléimides, inhibiteurs potentiels de l'intégrase du VIH-1 : Design, synthesis and pharmacological evaluation of maleimides, potential inhibitors of HIV-1 integrase.
Degree: Docteur es, Sciences de la vie et de la santé, spécialité Chimie organique, 2012, Université François-Rabelais de Tours
URL: http://www.theses.fr/2012TOUR3801
► Notre équipe de recherche a récemment développé les premiers inhibiteurs d’une enzyme à DDE, la transposase MOS1, une enzyme analogue à l’intégrase du VIH-1. Ces…
(more)
▼ Notre équipe de recherche a récemment développé les premiers inhibiteurs d’une enzyme à DDE, la transposase MOS1, une enzyme analogue à l’intégrase du VIH-1. Ces molécules, de type bisfurylmaléimides, ont également montré une efficacité contre les activités enzymatiques de l’intégrase du VIH. En se basant sur ces résultats, nous avons entrepris la synthèse de nouveaux bisfurylmaléimides dans le but d’identifier de nouveaux inhibiteurs de l’intégrase et de proposer de nouvelles molécules dans la lutte contre le virus responsable du SIDA. L’originalité de notre démarche est l’utilisation de la transposase MOS1 comme modèle pour concevoir nos molécules. Les méthodologies utilisées pour accéder à ces molécules font essentiellement appel à la chimie catalysée par des métaux de transition mais aussi à des réactions de chimie hétérocyclique telles que des réactions de Mitsunobu, de Knoevenagel ou encore de Horner-Wadsworth-Emmons. L’ensemble des molécules synthétisées a fait l’objet d’une évaluation de leurs activités inhibitrices sur la transposase MOS1 et l’intégrase du VIH-1. Leurs propriétés antivirales contre le VIH ont également été évaluées. Parallèlement à ces travaux, nous nous sommes intéressés à la réactivité du noyau maléimide dans des réactions de couplage pallado-catalysées et plus particulièrement dans des couplages de Sonogashira.
Our research team has recently developed the first inhibitors of a DDE enzyme, MOS1 transposase, an enzyme similar to the HIV-1 integrase. These molecules, having a bisfurylmaleimide structure, also showed efficacy against enzymatic activities of HIV integrase. Based on these results, we undertook the synthesis of new bisfurylmaleimides in order to identify new integrase inhibitors and propose new molecules in the fight against the virus that causes AIDS. The originality of our approach is the use of MOS1 transposase as a model for designing our molecules. Methodologies used to access these molecules are essentially involving chemistry catalyzed by transition metals but also reactions of heterocyclic chemistry such as Mitsunobu, Knoevenagel or Horner-Wadsworth-Emmons reactions. All synthesized molecules has been evaluated for their inhibitory activities on the MOS1 transposase and HIV-1 integrase. Their antiviral properties against HIV were also evaluated. Parallel to this work, we investigated the reactivity of the maleimide core in palladium-catalyzed cross-coupling reactions especially in Sonogashira couplings.
Advisors/Committee Members: Viaud-Massuard, Marie-Claude (thesis director).
Subjects/Keywords: SIDA; VIH; Intégrase; Enzyme à DDE; Transposase; Inhibiteur; Maléimide; Pharmacomodulation; Synthèse multi-étape; Chimie hétérocyclique; Réaction de couplage pallado-catalysée; Modélisation moléculaire; Complexation; Évaluation pharmacologique; Photoactivation; AIDS; HIV; Integrase; DDE enzyme; Transposase; Inhibitor; Maleimide; Pharmacomodulation; Multi-step synthesis; Heterocyclic chemistry; Palladium-catalyzed cross-coupling reaction; Molecular modeling; Complexation; Pharmacological evaluation; Photoaffinity labeling
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APA ·
Chicago ·
MLA ·
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to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Souffrin, A. (2012). Conception, synthèse et évaluation pharmacologique de maléimides, inhibiteurs potentiels de l'intégrase du VIH-1 : Design, synthesis and pharmacological evaluation of maleimides, potential inhibitors of HIV-1 integrase. (Doctoral Dissertation). Université François-Rabelais de Tours. Retrieved from http://www.theses.fr/2012TOUR3801
Chicago Manual of Style (16th Edition):
Souffrin, Agathe. “Conception, synthèse et évaluation pharmacologique de maléimides, inhibiteurs potentiels de l'intégrase du VIH-1 : Design, synthesis and pharmacological evaluation of maleimides, potential inhibitors of HIV-1 integrase.” 2012. Doctoral Dissertation, Université François-Rabelais de Tours. Accessed January 20, 2021.
http://www.theses.fr/2012TOUR3801.
MLA Handbook (7th Edition):
Souffrin, Agathe. “Conception, synthèse et évaluation pharmacologique de maléimides, inhibiteurs potentiels de l'intégrase du VIH-1 : Design, synthesis and pharmacological evaluation of maleimides, potential inhibitors of HIV-1 integrase.” 2012. Web. 20 Jan 2021.
Vancouver:
Souffrin A. Conception, synthèse et évaluation pharmacologique de maléimides, inhibiteurs potentiels de l'intégrase du VIH-1 : Design, synthesis and pharmacological evaluation of maleimides, potential inhibitors of HIV-1 integrase. [Internet] [Doctoral dissertation]. Université François-Rabelais de Tours; 2012. [cited 2021 Jan 20].
Available from: http://www.theses.fr/2012TOUR3801.
Council of Science Editors:
Souffrin A. Conception, synthèse et évaluation pharmacologique de maléimides, inhibiteurs potentiels de l'intégrase du VIH-1 : Design, synthesis and pharmacological evaluation of maleimides, potential inhibitors of HIV-1 integrase. [Doctoral Dissertation]. Université François-Rabelais de Tours; 2012. Available from: http://www.theses.fr/2012TOUR3801
Oklahoma State University
29.
Sun, Tzeli Julia.
Photoactivated DNA cleavage, enzyme inactivation, bacterial inhibition, and viral inactivation by the cotton phytoalexin 2,7-dihydroxycadalene, isolation of phytoalexin-resistant mutants of the cotton pathogen Xanthomonas campestris pv. malvacearum, and characterization of the pathogen's mutability.
Degree: Biochemistry, 1987, Oklahoma State University
URL: http://hdl.handle.net/11244/14410
Subjects/Keywords: gossypium hirsutum; xanthomonas campestris pv. malvacearum; 2,7-dihydroxycadalene; lacinilene c; sesquiterpenoid phytoalexins; photosensitizer; dna cleavage; active oxygen; deoxyribonuclease I; malate dehydrogenase; cauliflower mosaic virus; brassica rapa; photoactivation; dna-protein cross-linking; infectivity; cyshis box; nucleic acid binding domain; phytoalexin; antiviral
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APA ·
Chicago ·
MLA ·
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CSE |
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to Zotero / EndNote / Reference
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APA (6th Edition):
Sun, T. J. (1987). Photoactivated DNA cleavage, enzyme inactivation, bacterial inhibition, and viral inactivation by the cotton phytoalexin 2,7-dihydroxycadalene, isolation of phytoalexin-resistant mutants of the cotton pathogen Xanthomonas campestris pv. malvacearum, and characterization of the pathogen's mutability. (Thesis). Oklahoma State University. Retrieved from http://hdl.handle.net/11244/14410
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Sun, Tzeli Julia. “Photoactivated DNA cleavage, enzyme inactivation, bacterial inhibition, and viral inactivation by the cotton phytoalexin 2,7-dihydroxycadalene, isolation of phytoalexin-resistant mutants of the cotton pathogen Xanthomonas campestris pv. malvacearum, and characterization of the pathogen's mutability.” 1987. Thesis, Oklahoma State University. Accessed January 20, 2021.
http://hdl.handle.net/11244/14410.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Sun, Tzeli Julia. “Photoactivated DNA cleavage, enzyme inactivation, bacterial inhibition, and viral inactivation by the cotton phytoalexin 2,7-dihydroxycadalene, isolation of phytoalexin-resistant mutants of the cotton pathogen Xanthomonas campestris pv. malvacearum, and characterization of the pathogen's mutability.” 1987. Web. 20 Jan 2021.
Vancouver:
Sun TJ. Photoactivated DNA cleavage, enzyme inactivation, bacterial inhibition, and viral inactivation by the cotton phytoalexin 2,7-dihydroxycadalene, isolation of phytoalexin-resistant mutants of the cotton pathogen Xanthomonas campestris pv. malvacearum, and characterization of the pathogen's mutability. [Internet] [Thesis]. Oklahoma State University; 1987. [cited 2021 Jan 20].
Available from: http://hdl.handle.net/11244/14410.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Sun TJ. Photoactivated DNA cleavage, enzyme inactivation, bacterial inhibition, and viral inactivation by the cotton phytoalexin 2,7-dihydroxycadalene, isolation of phytoalexin-resistant mutants of the cotton pathogen Xanthomonas campestris pv. malvacearum, and characterization of the pathogen's mutability. [Thesis]. Oklahoma State University; 1987. Available from: http://hdl.handle.net/11244/14410
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
30.
Hazel, Ilke van.
Molecular Evolution and Functional Characterization of the Visual Pigment Proteins of the Great Bowerbird (Chlamydera nuchalis) and Other Vertebrates.
Degree: 2012, University of Toronto
URL: http://hdl.handle.net/1807/43401
► Visual pigments are light sensitive receptors in the eye that form the basis of sensory visual transduction. This thesis presents three studies that explore visual…
(more)
▼ Visual pigments are light sensitive receptors in the eye that form the basis of sensory visual transduction. This thesis presents three studies that explore visual pigment proteins in vertebrates using a number of computational and experimental methods in an evolutionary framework. The objective is not only to identify, but also to experimentally investigate the functional consequences of genetic variation in vertebrate visual pigments. The focus is on great bowerbirds (Chlamydera nuchalis), which are a model system in visual ecology due to their spectacular behaviour of building and decorating courtship bowers. There are 4 chapters: Chapter 1 introduces background information on visual pigments and vision in birds. Among visual pigment types, the short-wavelength-sensitive (SWS1) pigments have garnered particular interest due to the broad spectral range among vertebrates and the importance of UV signals in communication. Chapter 2 investigates the evolutionary history of SWS1 in vertebrates with a view toward its utility as a phylogenetic marker. Chapter 3 investigates SWS1 evolution and short-wavelength vision in birds, with particular focus on C. nuchalis and its SWS1. The evolution of spectral tuning mechanisms mediating UV/violet vision in passerines and parrots is elucidated in this chapter using site-directed mutagenesis, protein expression, and phylogenetic recreation of ancestral opsins. While cone opsins mediate colour vision in bright light, the rhodopsin visual pigment contained in rod photoreceptors is critical for dim light vision. Detailed characterization of rhodopsin function has only been conducted on a few model systems. Chapter 4 examines C. nuchalis RH1 using a number of functional assays in addition to absorbance spectra, including hydroxylamine sensitivity and the rate of retinal release. This chapter includes an investigation into the role of amino acid mutations typical of dim-light adapted vertebrates, D83N and A292S, in regulating functional properties of bovine and avian RH1s using site-directed mutagenesis. Together these chapters describe naturally occurring mutations in visual pigments and explore the way they can influence visual perception. These represent one of the few investigations of visual pigments from a species that is not a model lab organism and form a significant contribution to the field of visual pigment biochemistry and evolution.
PhD
Advisors/Committee Members: Chang, Belinda, Ecology and Evolutionary Biology.
Subjects/Keywords: bowerbird; visual pigments; opsin; vertebrate phylogenetics; molecular marker; avian vision; visual behaviour; adaptation; mutagenesis; protein expression; short wavelength sensitive 1; SWS1; spectral tuning; evolution; ancestral sequence reconstruction; maximum likelihood; rhodopsin; rod opsin; RH1; retinal release; photoactivation cycle; dim light adaptation; molecular evolution; PAML; 0307; 0317; 0715; 0306
…variation in the photoactivation cycle and of rod
and cone visual pigments. This is followed by a…
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APA ·
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MLA ·
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CSE |
Export
to Zotero / EndNote / Reference
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APA (6th Edition):
Hazel, I. v. (2012). Molecular Evolution and Functional Characterization of the Visual Pigment Proteins of the Great Bowerbird (Chlamydera nuchalis) and Other Vertebrates. (Doctoral Dissertation). University of Toronto. Retrieved from http://hdl.handle.net/1807/43401
Chicago Manual of Style (16th Edition):
Hazel, Ilke van. “Molecular Evolution and Functional Characterization of the Visual Pigment Proteins of the Great Bowerbird (Chlamydera nuchalis) and Other Vertebrates.” 2012. Doctoral Dissertation, University of Toronto. Accessed January 20, 2021.
http://hdl.handle.net/1807/43401.
MLA Handbook (7th Edition):
Hazel, Ilke van. “Molecular Evolution and Functional Characterization of the Visual Pigment Proteins of the Great Bowerbird (Chlamydera nuchalis) and Other Vertebrates.” 2012. Web. 20 Jan 2021.
Vancouver:
Hazel Iv. Molecular Evolution and Functional Characterization of the Visual Pigment Proteins of the Great Bowerbird (Chlamydera nuchalis) and Other Vertebrates. [Internet] [Doctoral dissertation]. University of Toronto; 2012. [cited 2021 Jan 20].
Available from: http://hdl.handle.net/1807/43401.
Council of Science Editors:
Hazel Iv. Molecular Evolution and Functional Characterization of the Visual Pigment Proteins of the Great Bowerbird (Chlamydera nuchalis) and Other Vertebrates. [Doctoral Dissertation]. University of Toronto; 2012. Available from: http://hdl.handle.net/1807/43401
. 
Open Access Theses and Dissertations
