subject:(Phenylalanine)

University of Manitoba

1. Yudistira, Harry. PHENYLALANINE CATABOLISM IN BURKHOLDERIA CENOCEPACIA K56-2.

Degree: Microbiology, 2010, University of Manitoba

URL: http://hdl.handle.net/1993/4276

► Synthetic cystic fibrosis sputum medium (SCFM) is rich in amino acids and supports robust growth of Burkholderia cenocepacia, a member of the Burkholderia cepacia complex… (more)

Subjects/Keywords: Phenylalanine Catabolism; Burkholderia cenocepacia

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APA (6^th Edition):

Yudistira, H. (2010). PHENYLALANINE CATABOLISM IN BURKHOLDERIA CENOCEPACIA K56-2. (Masters Thesis). University of Manitoba. Retrieved from http://hdl.handle.net/1993/4276

Chicago Manual of Style (16^th Edition):

Yudistira, Harry. “PHENYLALANINE CATABOLISM IN BURKHOLDERIA CENOCEPACIA K56-2.” 2010. Masters Thesis, University of Manitoba. Accessed January 18, 2021. http://hdl.handle.net/1993/4276.

MLA Handbook (7^th Edition):

Yudistira, Harry. “PHENYLALANINE CATABOLISM IN BURKHOLDERIA CENOCEPACIA K56-2.” 2010. Web. 18 Jan 2021.

Vancouver:

Yudistira H. PHENYLALANINE CATABOLISM IN BURKHOLDERIA CENOCEPACIA K56-2. [Internet] [Masters thesis]. University of Manitoba; 2010. [cited 2021 Jan 18]. Available from: http://hdl.handle.net/1993/4276.

Council of Science Editors:

Yudistira H. PHENYLALANINE CATABOLISM IN BURKHOLDERIA CENOCEPACIA K56-2. [Masters Thesis]. University of Manitoba; 2010. Available from: http://hdl.handle.net/1993/4276

University of Rochester

2. Rajbhandary, Annada. Investigating the relationship between structure and self-assembling behavior of Fmoc-protected phenylalanine derivatives.

Degree: PhD, 2016, University of Rochester

URL: http://hdl.handle.net/1802/31450

► Low molecular weight (LMW) hydrogelators have been utilized for numerous biomedical applications in cell culture, drug delivery and tissue engineering. Fluorenylmethoxycarbonyl (Fmoc) protected phenylalanine (Fmoc-Phe)… (more)

▼ Low molecular weight (LMW) hydrogelators have been utilized for numerous biomedical applications in cell culture, drug delivery and tissue engineering. Fluorenylmethoxycarbonyl (Fmoc) protected phenylalanine (Fmoc-Phe) derivatives are a privileged class of LMW hydrogelators that have excellent gelling abilities and have found uses in various functional applications. Since a large number of LMW hydrogelators have been discovered serendipitously, further understanding of the molecular structure and conditions that drive self-assembly and hydrogelation processes is imperative for intelligent design of biomaterials. This thesis investigates the structural differences of molecules which facilitate various types of self-assembly and hydrogelation processes. The first project investigates new ways of forming hydrogels by simple dissolution in water. While most gelling processes either rely on toxic organic cosolvents or stringent pH conditions to induce hydrogelation, our investigated method of forming hydrogels in unbuffered water in the absence of harsh condition, is comparatively more biocompatible for uses in cell culture applications. We utilized a cationic Fmoc-Phe based hydrogelators that can form gels in water upon addition of salt. These hydrogelators also form homogenous nanotubes at high gel concentrations which are exclusively dependent on the presence of the cationic charges. In the second project, we investigated the importance of hydrogen bonding in LMW gelators in giving directionality to the one-dimensional (1D) fibrils. Self-assembly behavior of amino acid and peptoid derivatives of Fmoc-phe monomers were compared. It was found that while amino acid monomers prefer formation of one-dimensional (1D) fibrils, peptoid monomers prefer forming two-dimensional (2D) sheets in the absence of hydrogen bonds. Crystals of the variants were obtained in different solvent-water conditions which were analyzed via X-ray diffraction (XRD). The XRD data revealed stark differences in packing arrangements of Fmoc-Phe amino acid and peptoid monomers. Amino acid monomers preferred parallel π–π stacking whereas most peptoid monomers displayed antiparallel and intramolecular π–π stacking. Finally in the third project, we investigated the self-assembly behavior of peptide/peptoid hybrids of the most widely studied dipeptide, Fmoc-Phe-Phe to understand it’s self-assembly propensity in the absence of hydrogen bonds and changes in the spatial arrangement of side chains. Hybrid peptide/peptoid variants were able to self-assemble even in the presence of only single hydrogen bond although fibrillization was not possible in the absence of both hydrogen bonds. Although self-assembly of the hybrid variants to form fibrils was possible, the gelling ability of these variants were greatly reduced mostly due to the stark differences in the hydrogen bonding abilities and spatial arrangement of the side chains. Therefore, with the research work presented in this thesis, insight into understanding of the structural and spatial arrangement of…

Subjects/Keywords: Bioorganic chemistry; phenylalanine derivatives; self-assembling

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APA (6^th Edition):

Rajbhandary, A. (2016). Investigating the relationship between structure and self-assembling behavior of Fmoc-protected phenylalanine derivatives. (Doctoral Dissertation). University of Rochester. Retrieved from http://hdl.handle.net/1802/31450

Chicago Manual of Style (16^th Edition):

Rajbhandary, Annada. “Investigating the relationship between structure and self-assembling behavior of Fmoc-protected phenylalanine derivatives.” 2016. Doctoral Dissertation, University of Rochester. Accessed January 18, 2021. http://hdl.handle.net/1802/31450.

MLA Handbook (7^th Edition):

Rajbhandary, Annada. “Investigating the relationship between structure and self-assembling behavior of Fmoc-protected phenylalanine derivatives.” 2016. Web. 18 Jan 2021.

Vancouver:

Rajbhandary A. Investigating the relationship between structure and self-assembling behavior of Fmoc-protected phenylalanine derivatives. [Internet] [Doctoral dissertation]. University of Rochester; 2016. [cited 2021 Jan 18]. Available from: http://hdl.handle.net/1802/31450.

Council of Science Editors:

Rajbhandary A. Investigating the relationship between structure and self-assembling behavior of Fmoc-protected phenylalanine derivatives. [Doctoral Dissertation]. University of Rochester; 2016. Available from: http://hdl.handle.net/1802/31450

Jawaharlal Nehru University

3. Naga Prasad, S V. Studies on the Phenylalanine ammonia Lyase PAL Gene S of Arabidopsis Thaliana; -.

Degree: School of Life Science, 1996, Jawaharlal Nehru University

URL: http://shodhganga.inflibnet.ac.in/handle/10603/32243

None

References p.172-188

Advisors/Committee Members: Upadhaya,Kailash c.

Subjects/Keywords: Phenylalanine; Arabidopsis; Thaliana

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APA (6^th Edition):

Naga Prasad, S. V. (1996). Studies on the Phenylalanine ammonia Lyase PAL Gene S of Arabidopsis Thaliana; -. (Thesis). Jawaharlal Nehru University. Retrieved from http://shodhganga.inflibnet.ac.in/handle/10603/32243

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Naga Prasad, S V. “Studies on the Phenylalanine ammonia Lyase PAL Gene S of Arabidopsis Thaliana; -.” 1996. Thesis, Jawaharlal Nehru University. Accessed January 18, 2021. http://shodhganga.inflibnet.ac.in/handle/10603/32243.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Naga Prasad, S V. “Studies on the Phenylalanine ammonia Lyase PAL Gene S of Arabidopsis Thaliana; -.” 1996. Web. 18 Jan 2021.

Vancouver:

Naga Prasad SV. Studies on the Phenylalanine ammonia Lyase PAL Gene S of Arabidopsis Thaliana; -. [Internet] [Thesis]. Jawaharlal Nehru University; 1996. [cited 2021 Jan 18]. Available from: http://shodhganga.inflibnet.ac.in/handle/10603/32243.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Naga Prasad SV. Studies on the Phenylalanine ammonia Lyase PAL Gene S of Arabidopsis Thaliana; -. [Thesis]. Jawaharlal Nehru University; 1996. Available from: http://shodhganga.inflibnet.ac.in/handle/10603/32243

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

The Ohio State University

4. Wrona, Ronald Matthew. Clinical epidemiologic study of hyperphenylalaninemia .

Degree: PhD, Graduate School, 1977, The Ohio State University

URL: http://rave.ohiolink.edu/etdc/view?acc_num=osu1487065361260532

Subjects/Keywords: Health Sciences; Phenylalanine

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APA (6^th Edition):

Wrona, R. M. (1977). Clinical epidemiologic study of hyperphenylalaninemia . (Doctoral Dissertation). The Ohio State University. Retrieved from http://rave.ohiolink.edu/etdc/view?acc_num=osu1487065361260532

Chicago Manual of Style (16^th Edition):

Wrona, Ronald Matthew. “Clinical epidemiologic study of hyperphenylalaninemia .” 1977. Doctoral Dissertation, The Ohio State University. Accessed January 18, 2021. http://rave.ohiolink.edu/etdc/view?acc_num=osu1487065361260532.

MLA Handbook (7^th Edition):

Wrona, Ronald Matthew. “Clinical epidemiologic study of hyperphenylalaninemia .” 1977. Web. 18 Jan 2021.

Vancouver:

Wrona RM. Clinical epidemiologic study of hyperphenylalaninemia . [Internet] [Doctoral dissertation]. The Ohio State University; 1977. [cited 2021 Jan 18]. Available from: http://rave.ohiolink.edu/etdc/view?acc_num=osu1487065361260532.

Council of Science Editors:

Wrona RM. Clinical epidemiologic study of hyperphenylalaninemia . [Doctoral Dissertation]. The Ohio State University; 1977. Available from: http://rave.ohiolink.edu/etdc/view?acc_num=osu1487065361260532

Michigan State University

5. Mutatu, Washington. Mechanistic evaluation and substrate specificity analysis of a phenylalanine aminomutase.

Degree: PhD, Department of Chemistry, 2010, Michigan State University

URL: http://etd.lib.msu.edu/islandora/object/etd:10343

Subjects/Keywords: Phenylalanine – Analysis; Stereochemistry

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Mutatu, W. (2010). Mechanistic evaluation and substrate specificity analysis of a phenylalanine aminomutase. (Doctoral Dissertation). Michigan State University. Retrieved from http://etd.lib.msu.edu/islandora/object/etd:10343

Chicago Manual of Style (16^th Edition):

Mutatu, Washington. “Mechanistic evaluation and substrate specificity analysis of a phenylalanine aminomutase.” 2010. Doctoral Dissertation, Michigan State University. Accessed January 18, 2021. http://etd.lib.msu.edu/islandora/object/etd:10343.

MLA Handbook (7^th Edition):

Mutatu, Washington. “Mechanistic evaluation and substrate specificity analysis of a phenylalanine aminomutase.” 2010. Web. 18 Jan 2021.

Vancouver:

Mutatu W. Mechanistic evaluation and substrate specificity analysis of a phenylalanine aminomutase. [Internet] [Doctoral dissertation]. Michigan State University; 2010. [cited 2021 Jan 18]. Available from: http://etd.lib.msu.edu/islandora/object/etd:10343.

Council of Science Editors:

Mutatu W. Mechanistic evaluation and substrate specificity analysis of a phenylalanine aminomutase. [Doctoral Dissertation]. Michigan State University; 2010. Available from: http://etd.lib.msu.edu/islandora/object/etd:10343

University of New Mexico

6. He, Jingxuan. DEVELOPMENT AND APPLICATION OF AN EXPANDED STREPTOMYCES GENETIC CODE.

Degree: Department of Chemistry and Chemical Biology, 2016, University of New Mexico

URL: https://digitalrepository.unm.edu/chem_etds/57

► Actinobacteria, especially those of genus Streptomyces, are a prominent source of bioactive natural products. The ability to site-specifically incorporate unnatural amino acids (UAAs) into… (more)

▼ Actinobacteria, especially those of genus Streptomyces, are a prominent source of bioactive natural products. The ability to site-specifically incorporate unnatural amino acids (UAAs) into natural product biosynthetic enzymes and ribosomally derived peptides in these organisms would constitute a valuable tool for drug discovery and development. The work described in this dissertation focuses on development and application of an expanded Streptomyces genetic code, including development of UAA incorporation systems based on amber suppression and sense codon reassignment, structural diversification of the model thiopeptide natural product thiostrepton using UAAs, and mapping protein-protein interactions in type II polyketide biosynthetic enzymes using photocrosslinking UAAs. First, we developed an amber suppression-based system of site-specific incorporation of p-iodo-L-phenylalanine (pIPhe) and p-azido-L-phenylalanine (pAzPhe) into superfolder GFP (sfGFP) in the model natural product producer Streptomyces venezuelae ATCC 15439. Next, the rare leucine codon TTA was reassigned to encode pIPhe and p-benzoyl-L-phenylalanine (pBpa) in S. coelicolor J1681 (ΔbldA), in which the unique tRNA^Leu_UAA (bldA) that recognizes the TTA codon was deleted. In the S. venezuelae ΔbldA strain, we achieved 20-fold higher yields of UAA containing protein using the TTA reassignment system compared to the amber suppression-based system; and were able to incorporate up to 10 scattered or 5 tandem UAAs in a single protein using TTA reassignment. Finally, we have carried out preliminary work on two applications. In the first, we constructed and tested functionality of a system designed to incorporate pAzPhe into the actinorhodin ketosynthase β (KSβ) in S. coelicolor J1681 to interrogate protein-protein interactions in actinorhodin biosynthesis. In the second, we have begun developing a system for incorporation of UAAs into thiostrepton in the native producer Streptomyces laurentii ATCC 31255. Preliminary results confirm the functionality of amber suppression system in S. laurentii; and demonstrated development of a TipA-based fluorescent biosensor for detecting thiopeptide antibiotics in S. venezuelae. Work on these two applications has laid the foundation for development of tools to structurally diversify the ribosomally synthesized peptides and to address questions related to natural product biosynthesis and mechanism of action that are relevant to drug discovery and development. Advisors/Committee Members: Charles E. Melançon III, Debra Dunaway-Mariano, Fu-sen Liang, Jeremy S. Edwards.

Subjects/Keywords: unnatural amino acids; Streptomyces; p-azido-L-phenylalanine; p-iodo-L-phenylalanine; natural products; Chemistry

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

He, J. (2016). DEVELOPMENT AND APPLICATION OF AN EXPANDED STREPTOMYCES GENETIC CODE. (Doctoral Dissertation). University of New Mexico. Retrieved from https://digitalrepository.unm.edu/chem_etds/57

Chicago Manual of Style (16^th Edition):

He, Jingxuan. “DEVELOPMENT AND APPLICATION OF AN EXPANDED STREPTOMYCES GENETIC CODE.” 2016. Doctoral Dissertation, University of New Mexico. Accessed January 18, 2021. https://digitalrepository.unm.edu/chem_etds/57.

MLA Handbook (7^th Edition):

He, Jingxuan. “DEVELOPMENT AND APPLICATION OF AN EXPANDED STREPTOMYCES GENETIC CODE.” 2016. Web. 18 Jan 2021.

Vancouver:

He J. DEVELOPMENT AND APPLICATION OF AN EXPANDED STREPTOMYCES GENETIC CODE. [Internet] [Doctoral dissertation]. University of New Mexico; 2016. [cited 2021 Jan 18]. Available from: https://digitalrepository.unm.edu/chem_etds/57.

Council of Science Editors:

He J. DEVELOPMENT AND APPLICATION OF AN EXPANDED STREPTOMYCES GENETIC CODE. [Doctoral Dissertation]. University of New Mexico; 2016. Available from: https://digitalrepository.unm.edu/chem_etds/57

Michigan State University

7. Wanninayake, Udayanga. A mechanistic investigation of mio-based aminomutases.

Degree: 2013, Michigan State University

URL: http://etd.lib.msu.edu/islandora/object/etd:3339

►

Thesis Ph. D. Michigan State University. Chemistry - Doctor of Philosophy 2013.

β-Amino acids are biologically active compounds of interest in medicinal chemistry, which are… (more)

▼

Thesis Ph. D. Michigan State University. Chemistry - Doctor of Philosophy 2013.

β-Amino acids are biologically active compounds of interest in medicinal chemistry, which are used as precursors for the biosynthesis of several biologically active compounds such as taxol, andrimid, chondromides and C-1027. Also they are used as important precursors for the synthesis of β-lactams and β-peptides. A class I lyase-like family of aminomutases isomerizes (S)-α-arylalanines to the corresponding β-amino acids by exchange of the NH2/H pair. This family uses a 3,5-dihydro-5-methylidene-4H-imidazol-4-one (MIO) group within the active site to initiate the reaction. The structures of a phenylalanine aminomutases from Taxus canadensis (TcPAM) and from Pantoea agglomerans (PaPAM) have been determined at 2.4 Å and 1.7 Å resolution, respectively. TcPAM isomerizes (S)-α-phenylalanine to the (R)-β-isomer, and PaPAM isomerizes (S)-α-phenylalanine to the (S)-β-isomer by exchange of the NH2/H pair. Both enzymes share similar tertiary- and quaternary-structures and have several highly conserved aliphatic residues positioned analogously in their active sites for substrate recognition. The active site of the TcPAM structure was solved in complex with (E)-cinnamate, which functions as both a substrate and an intermediate. The structure of PaPAM was solved as α- and β-phenylpropanoid adducts, presumably with (S)-α- and (S)-β-phenylalanine. These covalent intermediates provide strong evidence that PaPAM reacts by an alkylamine elimination pathway (Hoffmann-type or E2 elimination process), which involves covalent attachment between MIO cofactor and the amino group of the substrate or the product. The results indicate that the conformation of the carbon skeleton of the (S)-phenylalanine substrate remains as one rotamer after elimination of the NH2/H pair. It is presumed that the the exocyclic C-N bond of the amine-MIO adduct rotates into position below the α- and β-carbon atoms of the cinnamate intermediate to complete the isomerization reaction. Thus, the X-ray crystal structure of PaPAM also confirms the inversion of configuration at each migration terminus during the isomerization of the α-amino acid substrate into its β-isomer.To account for the distinct (3R)-β-amino acid stereochemistry catalyzed by TcPAM, the cinnamate skeleton must rotate the C1−Cα and Cβ−Cipso bonds 180° in the active site prior to exchange and rebinding of the NH2/H pair to the cinnamate, an event that is not required for the corresponding acrylate intermediate in the PaPAM reaction. Moreover, the aromatic ring of the intermediate makes only one direct hydrophobic interaction with Leu-104. A L104A mutant of TcPAM demonstrated a ~ 1.5-fold increase in kcat and a decrease in KM values for sterically demanding 3′-methyl-α-phenylalanine and styryl-α-alanine substrates, compared to the kinetic parameters for wild type TcPAM. These parameters did not…

Advisors/Committee Members: Walker, Kevin D, Geiger, James E, Spence, Dana, Borhan, Babak.

Subjects/Keywords: Pharmaceutical chemistry; Stereochemistry; Chemistry; Biochemistry; Aminomutases; Mio-based aminomutases; Phenylalanine aminomutases; Phenylalanine isomerization; Taxus canadensis (TcPAM); Pantoea agglomerans (PaPAM); -Amino acids

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APA (6^th Edition):

Wanninayake, U. (2013). A mechanistic investigation of mio-based aminomutases. (Thesis). Michigan State University. Retrieved from http://etd.lib.msu.edu/islandora/object/etd:3339

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Wanninayake, Udayanga. “A mechanistic investigation of mio-based aminomutases.” 2013. Thesis, Michigan State University. Accessed January 18, 2021. http://etd.lib.msu.edu/islandora/object/etd:3339.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Wanninayake, Udayanga. “A mechanistic investigation of mio-based aminomutases.” 2013. Web. 18 Jan 2021.

Vancouver:

Wanninayake U. A mechanistic investigation of mio-based aminomutases. [Internet] [Thesis]. Michigan State University; 2013. [cited 2021 Jan 18]. Available from: http://etd.lib.msu.edu/islandora/object/etd:3339.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Wanninayake U. A mechanistic investigation of mio-based aminomutases. [Thesis]. Michigan State University; 2013. Available from: http://etd.lib.msu.edu/islandora/object/etd:3339

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Universidade Federal de Mato Grosso do Sul

8. Oliveira, Aurora Maria Rosa de. Proteínas de defesa em folhas de memora peregrina (miers) sandwith (bignoniaceae) – uma espécie invasora de pastagens .

Degree: 2009, Universidade Federal de Mato Grosso do Sul

URL: http://repositorio.cbc.ufms.br:8080/jspui/handle/123456789/2583

► A espécie Memora peregrina (Miers) Sandwith (Bignoniaceae), popularmente conhecida como ciganinha, é nativa dos Cerrados de Mato Grosso do Sul, Mato Grosso, Goiás e Minas… (more)

▼ A espécie Memora peregrina (Miers) Sandwith (Bignoniaceae), popularmente conhecida como ciganinha, é nativa dos Cerrados de Mato Grosso do Sul, Mato Grosso, Goiás e Minas Gerais. No seu ambiente natural a ocorrência desta planta é esparsa, restrita a clareiras. Em áreas desmatadas, cujo manejo não é feito corretamente, para formação de pastagens, esta espécie aparece competindo com forrageiras, chegando a dificultar a pastagem de bovinos, pois floresce, frutifica e produz sementes o ano todo. Os pecuaristas geralmente utilizam a poda mecânica para o controle da espécie; entretanto, este método elimina a parte aérea e fragmenta o sistema subterrâneo, estimulando o desenvolvimento desta estrutura, bem como disseminando propágulos de reprodução vegetativa, tornando a espécie mais competitiva, pois permite a ocupação da área manejada em curto espaço de tempo. O objetivo deste trabalho foi acompanhar a taxa de crescimento, a produção de proteínas solúveis totais e a atividade das enzimas 1,3 glucanase, peroxidase e fenilalanina amônia-liase em folhas de indivíduos podados e não podados de M.peregrina em duas áreas do Mato Grosso do Sul, com alto grau de infestação por esta espécie, com a finalidade de verificar o impacto da injúria mecânica sobre a síntese de proteínas de defesa. Os indivíduos de Memora peregrina submetidos à poda mecânica apresentaram uma maior taxa de crescimento do que os indivíduos não podados, em ambas as áreas estudadas, corroborando os resultados obtidos por outros autores. Os teores de proteínas solúveis e a atividade das peroxidases nos pHs 5,0, 7,0 e 8,6 foram maiores nas folhas nos indivíduos podados de M.peregrina do que nos indivíduos não podados, durante alguns períodos após a poda, que foram diferentes para cada grupo de macromoléculas analisadas. É sugerido que as variações observadas na atividade das peroxidases de folhas de indivíduos podados de M.peregrina pode ser uma resposta à poda mecânica (aumento das peroxidases básicas e neutras), bem como o resultado da participação das mesmas nos processos de crescimento (aumento das peroxidases ácidas). A atividade das enzimas β-1,3-glucanase e da fenilalanina-amônia-liase foi semelhante entre indivíduos de M.peregrina podados e não podados, indicando que a poda mecânica não é um fator indutor destes processos de defesa nesta espécie. Advisors/Committee Members: Marques, Maria Rita (advisor).

Subjects/Keywords: Plantas - desenvolvimento; Plants - development; Proteínas; Proteins; Fenilalanina; Phenylalanine; Cerrados; Pastagens; Pastures

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Oliveira, A. M. R. d. (2009). Proteínas de defesa em folhas de memora peregrina (miers) sandwith (bignoniaceae) – uma espécie invasora de pastagens . (Thesis). Universidade Federal de Mato Grosso do Sul. Retrieved from http://repositorio.cbc.ufms.br:8080/jspui/handle/123456789/2583

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Oliveira, Aurora Maria Rosa de. “Proteínas de defesa em folhas de memora peregrina (miers) sandwith (bignoniaceae) – uma espécie invasora de pastagens .” 2009. Thesis, Universidade Federal de Mato Grosso do Sul. Accessed January 18, 2021. http://repositorio.cbc.ufms.br:8080/jspui/handle/123456789/2583.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Oliveira, Aurora Maria Rosa de. “Proteínas de defesa em folhas de memora peregrina (miers) sandwith (bignoniaceae) – uma espécie invasora de pastagens .” 2009. Web. 18 Jan 2021.

Vancouver:

Oliveira AMRd. Proteínas de defesa em folhas de memora peregrina (miers) sandwith (bignoniaceae) – uma espécie invasora de pastagens . [Internet] [Thesis]. Universidade Federal de Mato Grosso do Sul; 2009. [cited 2021 Jan 18]. Available from: http://repositorio.cbc.ufms.br:8080/jspui/handle/123456789/2583.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Oliveira AMRd. Proteínas de defesa em folhas de memora peregrina (miers) sandwith (bignoniaceae) – uma espécie invasora de pastagens . [Thesis]. Universidade Federal de Mato Grosso do Sul; 2009. Available from: http://repositorio.cbc.ufms.br:8080/jspui/handle/123456789/2583

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Texas A&M University

9. Panay Escobar, Aram Joel. Mechanistic and Structural Studies of Phenylalanine Hydroxylase from Chromobacterium violaceum.

Degree: PhD, Biochemistry, 2011, Texas A&M University

URL: http://hdl.handle.net/1969.1/ETD-TAMU-2010-08-8584

► The phenylalanine hydroxylase from Chromobacterium violaceum (CvPheH) is a non-heme iron monooxygenase that catalyzes the hydroxylation of phenylalanine. This study presents the use of kinetic… (more)

▼ The phenylalanine hydroxylase from Chromobacterium violaceum (CvPheH) is a non-heme iron monooxygenase that catalyzes the hydroxylation of phenylalanine. This study presents the use of kinetic isotope effects (KIE) as mechanistic probes to compare the reactivity of CvPheH and that of the eukaryotic aromatic amino acid hydroxylases. This study also describes the use of different spectroscopic and kinetic techniques to identify the hydroxylating intermediate for this enzyme and the assignment of the NMR backbone resonances of CvPheH. Kinetic isotope effects on aromatic and benzylic hydroxylation were used to establish that bacterial and eukaryotic phenylalanine hydroxylases have similar reactivity. The observed KIE on aromatic hydroxylation of 1.4 was shown to be a combination of an inverse isotope effect on the hydroxylation of the amino acid and a normal isotope effect on a subsequent step in the reaction. An isotope effect on benzylic hydroxylation of 10 was found for CvPheH. This result establishes the similar reactivity for CvPheH and the eukaryotic aromatic amino acid hydroxylases and suggests the involvement of a common hydroxylating intermediate. Kinetic isotope effects were used to study the hydroxylation of the aliphatic substrate cyclohexylalanine. The Dkcat value with [1,2,2,3,3,4,4,5,5,6,6-2H11]- cyclohexylalanine is unity with wild-type CvPheH, suggesting that chemistry is not ratelimiting with this substrate. The intramolecular isotope effect calculated using [1,2,3,4,5,6-2H6]-cyclohexylalanine yields a value of 14. This result is evidence for the involvement of a reactive iron species capable of abstracting a hydrogen atom from the aliphatic carbon in cyclohexylalanine. Analysis of the CvPheH reaction using freeze-quench Mössbauer spectroscopy allowed the detection of an Fe(IV) species in the first turnover of the enzyme. Chemical quench and stopped-flow spectrophotometric methods were used to establish the kinetic competency of the Fe(IV) intermediate as the hydroxylating species. The NMR amide backbone resonances in the HSQC spectrum of CvPheH were assigned to the corresponding amino acid residues using a suite of TROSY-based threedimensional triple resonance experiments. We were able to assign 224 residues out of the 278 assignable residues in CvPheH, this constitutes 81 percent of the assignable protein sequence. Advisors/Committee Members: Fitzpatrick, Paul F. (advisor), Reinhart, Gregory D. (advisor), Sholtz, Marty J. (committee member), Kunkel, Gary (committee member).

Subjects/Keywords: Phenylalanine hydroxylase; Mechanistic studies; Isotope effects; NMR backbone assignment; ligand binding

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APA (6^th Edition):

Panay Escobar, A. J. (2011). Mechanistic and Structural Studies of Phenylalanine Hydroxylase from Chromobacterium violaceum. (Doctoral Dissertation). Texas A&M University. Retrieved from http://hdl.handle.net/1969.1/ETD-TAMU-2010-08-8584

Chicago Manual of Style (16^th Edition):

Panay Escobar, Aram Joel. “Mechanistic and Structural Studies of Phenylalanine Hydroxylase from Chromobacterium violaceum.” 2011. Doctoral Dissertation, Texas A&M University. Accessed January 18, 2021. http://hdl.handle.net/1969.1/ETD-TAMU-2010-08-8584.

MLA Handbook (7^th Edition):

Panay Escobar, Aram Joel. “Mechanistic and Structural Studies of Phenylalanine Hydroxylase from Chromobacterium violaceum.” 2011. Web. 18 Jan 2021.

Vancouver:

Panay Escobar AJ. Mechanistic and Structural Studies of Phenylalanine Hydroxylase from Chromobacterium violaceum. [Internet] [Doctoral dissertation]. Texas A&M University; 2011. [cited 2021 Jan 18]. Available from: http://hdl.handle.net/1969.1/ETD-TAMU-2010-08-8584.

Council of Science Editors:

Panay Escobar AJ. Mechanistic and Structural Studies of Phenylalanine Hydroxylase from Chromobacterium violaceum. [Doctoral Dissertation]. Texas A&M University; 2011. Available from: http://hdl.handle.net/1969.1/ETD-TAMU-2010-08-8584

10. Li, Jun. Structural Studies of the Catalytic and Regulatory Mechanisms of Phenylalanine Hydroxylase.

Degree: PhD, Biochemistry, 2011, Texas A&M University

URL: http://hdl.handle.net/1969.1/ETD-TAMU-2010-08-8285

► The catalytic and regulatory mechanisms of phenylalanine hydroxylase were investigated by structural studies of in this research. Phenylalanine hydroxylase (PheH) hydroxylates phenylalanine to produce tyrosine… (more)

▼ The catalytic and regulatory mechanisms of phenylalanine hydroxylase were investigated by structural studies of in this research. Phenylalanine hydroxylase (PheH) hydroxylates phenylalanine to produce tyrosine using tetrahydrobiopterin (BH4) and oxygen. The three ligands to the iron, His285, His290, and Glu330, were mutated to glutamine, glutamate, and histidine. All the mutants had low but measurable activity. Mutation of Glu330 had the greatest effect on activity and mutation of His290 the least. All the mutations resulted in an excess of tetrahydropterin oxidized relative to tyrosine formation, with mutation of His285 having the greatest effect on the coupling of the two partial reactions. All the mutants greatly decreased the affinity for iron, with mutation of Glu330 the most deleterious. The results complement previous results with tyrosine hydroxylase in establishing the plasticity of the individual iron ligands in this enzyme family. Hydrogen/deuterium exchange and mass spectrometry showed that peptides lying in the interface between the regulatory and catalytic domains display large increases of deuterium incorporation in the presence of phenylalanine. However, the effects of phenylalanine on a mutant enzyme lacking the regulatory domain are limited to peptides surrounding the binding site of phenylalanine. These results support the autoinhibitory function of the N-terminus of PheH. No peptides show a changed deuterium incorporation pattern in the presence of BH4, suggesting that BH4 binding does not change the structure significantly from the resting form. In phosphorylated PheH, three peptides show a deuterium incorporation pattern similar to that of unphosphorylated PheH plus phenylalanine, while the other peptides sensitive to phenylalanine binding in unphosphorylated PheH show the same pattern as that of unphosphorylated PheH without phenylalanine. Therefore, the conformational changes induced by phosphorylation are similar to but not identical to those associated with phenylalanine activation. The isolated regulatory domain (PheH1-117) was expressed and purified using a QSepharose column followed by a gel filtration column. Analytical gel filtration shows that PheH1-117 exists as a dimer in solution. In the presence of phenylalanine, the retention time of PheH1-117 is significantly changed. The 1H-15N NMR spectra of PheH1- 117 show that the cross-peaks of several residues are altered in the presence of phenylalanine. These results support the existence of a regulatory binding site for phenylalanine in the regulatory domain of PheH. Advisors/Committee Members: Fitzpatrick, Paul (advisor), Reinhart, Gregory (committee member), Kunkel, Gary (committee member), Raushel, Frank (committee member).

Subjects/Keywords: Phenylalanine hydroxylase; Structural study

…phenylalanine hydroxylase ........... 4 Figure 1.2. The position of the N-terminal sequence (… …presence of phenylalanine................................................ 65 Peptides of wild… …phenylalanine .............. 68 Comparison of deuterium incorporation of wild-type PheH in the… …absence and presence of phenylalanine after 2 h ................................. 70 The… …peptides showing increased deuterium incorporation in the presence of phenylalanine…

10 more images…

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APA (6^th Edition):

Li, J. (2011). Structural Studies of the Catalytic and Regulatory Mechanisms of Phenylalanine Hydroxylase. (Doctoral Dissertation). Texas A&M University. Retrieved from http://hdl.handle.net/1969.1/ETD-TAMU-2010-08-8285

Chicago Manual of Style (16^th Edition):

Li, Jun. “Structural Studies of the Catalytic and Regulatory Mechanisms of Phenylalanine Hydroxylase.” 2011. Doctoral Dissertation, Texas A&M University. Accessed January 18, 2021. http://hdl.handle.net/1969.1/ETD-TAMU-2010-08-8285.

MLA Handbook (7^th Edition):

Li, Jun. “Structural Studies of the Catalytic and Regulatory Mechanisms of Phenylalanine Hydroxylase.” 2011. Web. 18 Jan 2021.

Vancouver:

Li J. Structural Studies of the Catalytic and Regulatory Mechanisms of Phenylalanine Hydroxylase. [Internet] [Doctoral dissertation]. Texas A&M University; 2011. [cited 2021 Jan 18]. Available from: http://hdl.handle.net/1969.1/ETD-TAMU-2010-08-8285.

Council of Science Editors:

Li J. Structural Studies of the Catalytic and Regulatory Mechanisms of Phenylalanine Hydroxylase. [Doctoral Dissertation]. Texas A&M University; 2011. Available from: http://hdl.handle.net/1969.1/ETD-TAMU-2010-08-8285

University of Oxford

11. Lopez, Raul Cesar Gerardo. Studies on the synthesis of the cofactor, methoxatin and on simple thioaldehydes.

Degree: PhD, 1982, University of Oxford

URL: http://ora.ox.ac.uk/objects/uuid:a5bf53c8-bb79-49d3-a635-2a4d074da99f ; http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.344292

Subjects/Keywords: 547; Coenzymes : Aldehydes : Phenylalanine

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APA (6^th Edition):

Lopez, R. C. G. (1982). Studies on the synthesis of the cofactor, methoxatin and on simple thioaldehydes. (Doctoral Dissertation). University of Oxford. Retrieved from http://ora.ox.ac.uk/objects/uuid:a5bf53c8-bb79-49d3-a635-2a4d074da99f ; http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.344292

Chicago Manual of Style (16^th Edition):

Lopez, Raul Cesar Gerardo. “Studies on the synthesis of the cofactor, methoxatin and on simple thioaldehydes.” 1982. Doctoral Dissertation, University of Oxford. Accessed January 18, 2021. http://ora.ox.ac.uk/objects/uuid:a5bf53c8-bb79-49d3-a635-2a4d074da99f ; http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.344292.

MLA Handbook (7^th Edition):

Lopez, Raul Cesar Gerardo. “Studies on the synthesis of the cofactor, methoxatin and on simple thioaldehydes.” 1982. Web. 18 Jan 2021.

Vancouver:

Lopez RCG. Studies on the synthesis of the cofactor, methoxatin and on simple thioaldehydes. [Internet] [Doctoral dissertation]. University of Oxford; 1982. [cited 2021 Jan 18]. Available from: http://ora.ox.ac.uk/objects/uuid:a5bf53c8-bb79-49d3-a635-2a4d074da99f ; http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.344292.

Council of Science Editors:

Lopez RCG. Studies on the synthesis of the cofactor, methoxatin and on simple thioaldehydes. [Doctoral Dissertation]. University of Oxford; 1982. Available from: http://ora.ox.ac.uk/objects/uuid:a5bf53c8-bb79-49d3-a635-2a4d074da99f ; http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.344292

Iowa State University

12. Baker, Robert Swain. Response of Lactobacillus arabinosus to p-fluorophenylalanine.

Degree: 1953, Iowa State University

URL: https://lib.dr.iastate.edu/rtd/13084

Subjects/Keywords: Phenylalanine; Lactobacillus plantarum; Biochemistry

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APA (6^th Edition):

Baker, R. S. (1953). Response of Lactobacillus arabinosus to p-fluorophenylalanine. (Thesis). Iowa State University. Retrieved from https://lib.dr.iastate.edu/rtd/13084

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Baker, Robert Swain. “Response of Lactobacillus arabinosus to p-fluorophenylalanine.” 1953. Thesis, Iowa State University. Accessed January 18, 2021. https://lib.dr.iastate.edu/rtd/13084.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Baker, Robert Swain. “Response of Lactobacillus arabinosus to p-fluorophenylalanine.” 1953. Web. 18 Jan 2021.

Vancouver:

Baker RS. Response of Lactobacillus arabinosus to p-fluorophenylalanine. [Internet] [Thesis]. Iowa State University; 1953. [cited 2021 Jan 18]. Available from: https://lib.dr.iastate.edu/rtd/13084.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Baker RS. Response of Lactobacillus arabinosus to p-fluorophenylalanine. [Thesis]. Iowa State University; 1953. Available from: https://lib.dr.iastate.edu/rtd/13084

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of South Florida

13. Kelly, Katrina E. Synthesis, Oxidation, and Distribution of Polyphenols in Strawberry Fruit During Cold Storage.

Degree: 2018, University of South Florida

URL: https://scholarcommons.usf.edu/etd/7314

► Plants inherently produce polyphenols (i.e., antioxidants) as a response to reduce oxidative stress caused by abusive environmental pre- and postharvest conditions. These antioxidants, as well… (more)

▼ Plants inherently produce polyphenols (i.e., antioxidants) as a response to reduce oxidative stress caused by abusive environmental pre- and postharvest conditions. These antioxidants, as well as vitamin C, are present in considerable levels in strawberries; however, excessive oxidative stress brought on by improper postharvest handling conditions can reduce the levels of antioxidants in the fruit and shorten the shelf-life of strawberries. Nevertheless, it may be possible to utilize strawberry’s naturally occurring polyphenols to reduce postharvest stress and extend their shelf life. The polyphenolic profile has been previously investigated in several strawberry cultivars, however no studies have determined the unique polyphenolic profiles of three important Florida strawberry cultivars (‘Florida Radiance’, Sweet Sensation® ‘Florida 127’and ‘Florida Beauty’) at harvest and during cold storage. Therefore, in order to better understand the distribution of individual polyphenols within these cultivars and their impact on postharvest shelf-life, this study examined the polyphenolic profiles throughout 7 days of cold storage (1 °C) using an HPLC-DAD. The activity of phenylalanine ammonia lyase (PAL), a key enzyme in the biosynthesis of polyphenols, and polyphenol oxidase (PPO), the enzyme responsible for polyphenol degradation, were also examined during cold storage to understand their possible influences on postharvest synthesis or degradation of polyphenols. This study revealed that the polyphenolic profile of strawberry fruit was genotype dependent; however, pelargonidin 3-glucoside was consistently the anthocyanin found in higher concentrations in the fruit regardless of the cultivar. Apart from the anthocyanins, the flavonols showed the most variation among the three cultivars. PAL was slightly induced during strawberry postharvest storage suggesting that a stress response occurred during cold storage while PPO showed variable induction patterns across all three cultivars most likely due to their different polyphenol profiles. Analysis of the distribution of polyphenols in the cortex and pith of strawberries showed that polyphenols were mostly concentrated in the cortex of the fruit and that the concentration of individual polyphenol in each fruit tissue varied by cultivar. These results indicate that the oxidative stress response varies in each of the strawberry cultivars studied contributing to their unique polyphenolic profile. Results from this study can ultimately help to identify the polyphenols and enzymes related to superior postharvest quality in future studies.

Subjects/Keywords: abiotic stress; anthocyanins; phenylalanine ammonia-lyase; polyphenol oxidase; polyphenols; strawberry; Biology

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APA (6^th Edition):

Kelly, K. E. (2018). Synthesis, Oxidation, and Distribution of Polyphenols in Strawberry Fruit During Cold Storage. (Thesis). University of South Florida. Retrieved from https://scholarcommons.usf.edu/etd/7314

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Kelly, Katrina E. “Synthesis, Oxidation, and Distribution of Polyphenols in Strawberry Fruit During Cold Storage.” 2018. Thesis, University of South Florida. Accessed January 18, 2021. https://scholarcommons.usf.edu/etd/7314.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Kelly, Katrina E. “Synthesis, Oxidation, and Distribution of Polyphenols in Strawberry Fruit During Cold Storage.” 2018. Web. 18 Jan 2021.

Vancouver:

Kelly KE. Synthesis, Oxidation, and Distribution of Polyphenols in Strawberry Fruit During Cold Storage. [Internet] [Thesis]. University of South Florida; 2018. [cited 2021 Jan 18]. Available from: https://scholarcommons.usf.edu/etd/7314.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Kelly KE. Synthesis, Oxidation, and Distribution of Polyphenols in Strawberry Fruit During Cold Storage. [Thesis]. University of South Florida; 2018. Available from: https://scholarcommons.usf.edu/etd/7314

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Georgia Tech

14. Sirimanne, Sarath Ranjith. Syntheses and enzymatic studies of novel substrate analogs of phenylalanine hydroxylase and dopamine beta monooxygenase.

Degree: PhD, Chemistry and biochemistry, 1988, Georgia Tech

URL: http://hdl.handle.net/1853/30937

Subjects/Keywords: Dopamine; Phenylalanine; Organic compounds Synthesis

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APA (6^th Edition):

Sirimanne, S. R. (1988). Syntheses and enzymatic studies of novel substrate analogs of phenylalanine hydroxylase and dopamine beta monooxygenase. (Doctoral Dissertation). Georgia Tech. Retrieved from http://hdl.handle.net/1853/30937

Chicago Manual of Style (16^th Edition):

Sirimanne, Sarath Ranjith. “Syntheses and enzymatic studies of novel substrate analogs of phenylalanine hydroxylase and dopamine beta monooxygenase.” 1988. Doctoral Dissertation, Georgia Tech. Accessed January 18, 2021. http://hdl.handle.net/1853/30937.

MLA Handbook (7^th Edition):

Sirimanne, Sarath Ranjith. “Syntheses and enzymatic studies of novel substrate analogs of phenylalanine hydroxylase and dopamine beta monooxygenase.” 1988. Web. 18 Jan 2021.

Vancouver:

Sirimanne SR. Syntheses and enzymatic studies of novel substrate analogs of phenylalanine hydroxylase and dopamine beta monooxygenase. [Internet] [Doctoral dissertation]. Georgia Tech; 1988. [cited 2021 Jan 18]. Available from: http://hdl.handle.net/1853/30937.

Council of Science Editors:

Sirimanne SR. Syntheses and enzymatic studies of novel substrate analogs of phenylalanine hydroxylase and dopamine beta monooxygenase. [Doctoral Dissertation]. Georgia Tech; 1988. Available from: http://hdl.handle.net/1853/30937

University of Bath

15. Woodford, Andrew. Modelling the skin and systemic dispositions of amino acids to assess the potential for transdermal, non-invasive monitoring : phenylalanine as a case study.

Degree: PhD, 2017, University of Bath

URL: https://researchportal.bath.ac.uk/en/studentthesis/modelling-the-skin-and-systemic-dispositions-of-amino-acids-to-assess-the-potential-for-transdermal-noninvasive-monitoring-phenylalanine-as-a-case-study(b4da8b48-e9f4-4d3f-964d-ae0f116cb575).html ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.715255

► This thesis investigates the potential for monitoring current and historic blood serum concentrations of amino acids via transdermal extraction using phenylalanine as a case study.… (more)

Subjects/Keywords: 616; Reverse iontophoresis; Transdermal delivery; Phenylalanine; amino acid

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APA (6^th Edition):

Woodford, A. (2017). Modelling the skin and systemic dispositions of amino acids to assess the potential for transdermal, non-invasive monitoring : phenylalanine as a case study. (Doctoral Dissertation). University of Bath. Retrieved from https://researchportal.bath.ac.uk/en/studentthesis/modelling-the-skin-and-systemic-dispositions-of-amino-acids-to-assess-the-potential-for-transdermal-noninvasive-monitoring-phenylalanine-as-a-case-study(b4da8b48-e9f4-4d3f-964d-ae0f116cb575).html ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.715255

Chicago Manual of Style (16^th Edition):

Woodford, Andrew. “Modelling the skin and systemic dispositions of amino acids to assess the potential for transdermal, non-invasive monitoring : phenylalanine as a case study.” 2017. Doctoral Dissertation, University of Bath. Accessed January 18, 2021. https://researchportal.bath.ac.uk/en/studentthesis/modelling-the-skin-and-systemic-dispositions-of-amino-acids-to-assess-the-potential-for-transdermal-noninvasive-monitoring-phenylalanine-as-a-case-study(b4da8b48-e9f4-4d3f-964d-ae0f116cb575).html ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.715255.

MLA Handbook (7^th Edition):

Woodford, Andrew. “Modelling the skin and systemic dispositions of amino acids to assess the potential for transdermal, non-invasive monitoring : phenylalanine as a case study.” 2017. Web. 18 Jan 2021.

Vancouver:

Woodford A. Modelling the skin and systemic dispositions of amino acids to assess the potential for transdermal, non-invasive monitoring : phenylalanine as a case study. [Internet] [Doctoral dissertation]. University of Bath; 2017. [cited 2021 Jan 18]. Available from: https://researchportal.bath.ac.uk/en/studentthesis/modelling-the-skin-and-systemic-dispositions-of-amino-acids-to-assess-the-potential-for-transdermal-noninvasive-monitoring-phenylalanine-as-a-case-study(b4da8b48-e9f4-4d3f-964d-ae0f116cb575).html ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.715255.

Council of Science Editors:

Woodford A. Modelling the skin and systemic dispositions of amino acids to assess the potential for transdermal, non-invasive monitoring : phenylalanine as a case study. [Doctoral Dissertation]. University of Bath; 2017. Available from: https://researchportal.bath.ac.uk/en/studentthesis/modelling-the-skin-and-systemic-dispositions-of-amino-acids-to-assess-the-potential-for-transdermal-noninvasive-monitoring-phenylalanine-as-a-case-study(b4da8b48-e9f4-4d3f-964d-ae0f116cb575).html ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.715255

University of British Columbia

16. Sá, Mário Moniz de. Induction of phenylpropanoid metabolism in elicitor-treated hybrid poplar suspension-cultured cells.

Degree: MS- MSc, Botany, 1991, University of British Columbia

URL: http://hdl.handle.net/2429/30296

► Induction of phenylpropanoid metabolism in many plants is associated with the induction of plant defence responses. Among these are the accumulation of phenylpropanoid-derived phytoalexins, increase… (more)

Subjects/Keywords: Plant defenses; Phenylalanine – Metabolism

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APA (6^th Edition):

Sá, M. M. d. (1991). Induction of phenylpropanoid metabolism in elicitor-treated hybrid poplar suspension-cultured cells. (Masters Thesis). University of British Columbia. Retrieved from http://hdl.handle.net/2429/30296

Chicago Manual of Style (16^th Edition):

Sá, Mário Moniz de. “Induction of phenylpropanoid metabolism in elicitor-treated hybrid poplar suspension-cultured cells.” 1991. Masters Thesis, University of British Columbia. Accessed January 18, 2021. http://hdl.handle.net/2429/30296.

MLA Handbook (7^th Edition):

Sá, Mário Moniz de. “Induction of phenylpropanoid metabolism in elicitor-treated hybrid poplar suspension-cultured cells.” 1991. Web. 18 Jan 2021.

Vancouver:

Sá MMd. Induction of phenylpropanoid metabolism in elicitor-treated hybrid poplar suspension-cultured cells. [Internet] [Masters thesis]. University of British Columbia; 1991. [cited 2021 Jan 18]. Available from: http://hdl.handle.net/2429/30296.

Council of Science Editors:

Sá MMd. Induction of phenylpropanoid metabolism in elicitor-treated hybrid poplar suspension-cultured cells. [Masters Thesis]. University of British Columbia; 1991. Available from: http://hdl.handle.net/2429/30296

University of British Columbia

17. Camm, Edith Ellen. Tyrosine and phenylalanine ammonia lyases in Sporobolomyces roseus.

Degree: MS- MSc, Botany, 1968, University of British Columbia

URL: http://hdl.handle.net/2429/35836

► The enzymes phenylalanine ammonia lyase (PAL) and tyrosine ammonia lyase (TAL) were studied in the yeast Sporobolomyces roseus (Kluyver and van Neil). Cells grown on… (more)

Subjects/Keywords: Sporobolomyces roseus; Tyrosine; Phenylalanine

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APA (6^th Edition):

Camm, E. E. (1968). Tyrosine and phenylalanine ammonia lyases in Sporobolomyces roseus. (Masters Thesis). University of British Columbia. Retrieved from http://hdl.handle.net/2429/35836

Chicago Manual of Style (16^th Edition):

Camm, Edith Ellen. “Tyrosine and phenylalanine ammonia lyases in Sporobolomyces roseus.” 1968. Masters Thesis, University of British Columbia. Accessed January 18, 2021. http://hdl.handle.net/2429/35836.

MLA Handbook (7^th Edition):

Camm, Edith Ellen. “Tyrosine and phenylalanine ammonia lyases in Sporobolomyces roseus.” 1968. Web. 18 Jan 2021.

Vancouver:

Camm EE. Tyrosine and phenylalanine ammonia lyases in Sporobolomyces roseus. [Internet] [Masters thesis]. University of British Columbia; 1968. [cited 2021 Jan 18]. Available from: http://hdl.handle.net/2429/35836.

Council of Science Editors:

Camm EE. Tyrosine and phenylalanine ammonia lyases in Sporobolomyces roseus. [Masters Thesis]. University of British Columbia; 1968. Available from: http://hdl.handle.net/2429/35836

University of Stirling

18. Griffiths, Peter V. Treatment factors and neuropsychological outcome in phenylketonuria.

Degree: PhD, 1997, University of Stirling

URL: http://hdl.handle.net/1893/22863

► Phenylketonuria (PKU) is an inherited metabolic disease that affects about one in 10,000 of the population worldwide. In the classical form of the condition, the… (more)

▼ Phenylketonuria (PKU) is an inherited metabolic disease that affects about one in 10,000 of the population worldwide. In the classical form of the condition, the hepatic enzyme phenylalanine hydroxlase is absent or much reduced. If untreated, severe or profound mental handicap customarily results due to the accumulation of dietary phenylalanine (phe) which is neurotoxic. The mechanism by which phe impairs growth in the immature nervous system is little understood, but myelin metabolism appears to be disturbed. Treatment is by reduction of phe in daily food intake. Treatment should ideally begin in the neonatal period if intellectual loss is to be avoided. However, the safe range of phe concentrations during treatment and the age at which treatment can be discontinued without further damage being inflicted are uncertain. The studies reported in this volume investigated neuropsychological outcomes of treatment control and cessation factors. In addition, the question of whether executive functions are especially vulnerable to elevated phe concentrations during treatment was addressed. Patient samples conformed to the practice adopted in the West of Scotland regional centre for the management of PKU of maintaining dietary treatment until age 10 or beyond. Almost exclusively, negative findings emerged. These suggested that, if control of phe intake conforms to current UK recommendations for the preschool and primary years, neither global nor specific intellectual deficit result. Furthermore, the data supported the view that cessation of treatment at 10 years of age does not have harmful consequences. These findings have direct implications for the formulation of clinical policy on the treatment of PKU, but it must be recognized that the history of the successful treatment of PKU and mass screening for the disease spans a mere three decades. Thus, treatment outcome research to date is based only on children and young adults. In future investigations, a life-span approach will be required before the issues raised in this thesis can be finally settled.

Subjects/Keywords: Phenylketonuria; Phenylalanine Metabolism; Metabolism Disorders

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APA (6^th Edition):

Griffiths, P. V. (1997). Treatment factors and neuropsychological outcome in phenylketonuria. (Doctoral Dissertation). University of Stirling. Retrieved from http://hdl.handle.net/1893/22863

Chicago Manual of Style (16^th Edition):

Griffiths, Peter V. “Treatment factors and neuropsychological outcome in phenylketonuria.” 1997. Doctoral Dissertation, University of Stirling. Accessed January 18, 2021. http://hdl.handle.net/1893/22863.

MLA Handbook (7^th Edition):

Griffiths, Peter V. “Treatment factors and neuropsychological outcome in phenylketonuria.” 1997. Web. 18 Jan 2021.

Vancouver:

Griffiths PV. Treatment factors and neuropsychological outcome in phenylketonuria. [Internet] [Doctoral dissertation]. University of Stirling; 1997. [cited 2021 Jan 18]. Available from: http://hdl.handle.net/1893/22863.

Council of Science Editors:

Griffiths PV. Treatment factors and neuropsychological outcome in phenylketonuria. [Doctoral Dissertation]. University of Stirling; 1997. Available from: http://hdl.handle.net/1893/22863

19. Κουρέλης, Θεόδωρος. Σύνθεση πεπτιδικών αναλόγων της χλωραμφαινικόλης και μελέτη της βιολογικής τους δραστικότητας.

Degree: 2009, University of Patras

URL: http://nemertes.lis.upatras.gr/jspui/handle/10889/2460

► Στην παρούσα εργασία συνθέσαμε ένα άμινο-άκυλο- και ένα πεπτίδυλο- ανάλογο της χλωραμφαινικόλης. Τα ανάλογα αυτά ήταν η β-αλανίνη-χλωραμφαινικόλη (β-alaCAM) και η φαινυλαλανίνη-φαινυλαλανίνη-χλωραμφαινικόλη (PhePheCAM). Στην συνέχεια… (more)

▼ Στην παρούσα εργασία συνθέσαμε ένα άμινο-άκυλο- και ένα πεπτίδυλο- ανάλογο της χλωραμφαινικόλης. Τα ανάλογα αυτά ήταν η β-αλανίνη-χλωραμφαινικόλη (β-alaCAM) και η φαινυλαλανίνη-φαινυλαλανίνη-χλωραμφαινικόλη (PhePheCAM). Στην συνέχεια μελετήσαμε την βιολογική συμπεριφορά των αναλόγων αυτών μέσα από την μελέτη της κινητικής της αναστολής του σχηματισμού πεπτιδικού δεσμού που επιφέρουν τα εν λόγω ανάλογα. Σε πρωτεϊνοσυνθετικό σύστημα ριβοσωμάτων εκπορευόμενων από Escherichia coli η σύνθεση ακέτυλο-φαινυλαλάνυλο-πουρομυκίνης πραγματοποιείται μέσω μιας αντίδρασης ψευδοπρώτης τάξεως μεταξύ συμπλέγματος C, δηλαδή ακέτυλο-φαινυλαλάνυλο-poly(U)-ριβοσωμάτων, και περίσσειας πουρομυκίνης. Τόσο η β-alaCAM, όσο και η PhePheCAM μελετήθηκαν ως αναστολείς της αντίδρασης σύνθεσης ακέτυλο-φαινυλαλάνυλο-πουρομυκίνης και τα αποτελέσματα της κινητικής της αναστολής που επέφεραν συγκρίθηκαν με γνωστά από την βιβλιογραφία αντίστοιχα αποτελέσματα που αφορούν τόσο την μητρική ένωση, όσο και άλλα άμινο-άκυλο- και πεπτιδικά ανάλογα αυτής. Αρχικά παρατηρήσαμε ότι, απουσία αναστολέα, η αντίδραση ακολουθεί κινητική πρώτης τάξεως καθόλη την χρονική διάρκεια της χημικής αντίδρασης. Ωστόσο, στη συνέχεια παρατηρήσαμε ότι η παρουσία τόσο της β-alaCAM, όσο και της PhePheCAM είχε σαν αποτέλεσμα διφασικές λογαριθμικές συναρτήσεις συγκέντρωσης – χρόνου, όπου υφίστατο μία αρχική ή πρώτη χρονική φάση και μία τελική ή δεύτερη χρονική φάση της χημικής αντίδρασης πουρομυκίνης. Ακολούθησε λεπτομερής κινητική ανάλυση, αρχικά μέσω διαγραμμάτων διπλού αντιστρόφου για τις αρχικές και τις τελικές κλίσεις των λογαριθμικών χρονοκαμπυλών, καθώς και στη συνέχεια μέσω επαναδιαγραμμάτων αρχικών και τελικών κλίσεων έναντι της συγκέντρωσης του αναστολέα. Με τον τρόπο αυτό υπολογίστηκαν οι κινητικές σταθερές αναστολής Κi οι οποίες και συγκρίθηκαν με την κινητική σταθερά αναστολής της μητρικής ένωσης. Τέλος, μέσω υπολογιστικού προγράμματος προσομοίωσης, σχεδιάστηκαν οι συναρτήσεις της φαινομενικής σταθεράς εξισορρόπησης keq έναντι της συγκέντρωσης του αναστολέα και υπολογίστηκαν οι σταθερές k6 και k7. Tόσο η β-alaCAM όσο και η PhePheCAM εμφάνισαν συμπεριφορά βραδέως προσδενομένου συναγωνιστικού αναστολέα ανεξάρτητα από την συγκέντρωσή τους, σε αντίθεση με την μητρική ένωση η οποία εμφανίζει συμπεριφορά συναγωνιστικού αναστολέα σε μικρές συγκεντρώσεις αυτής και συμπεριφορά μικτού μη-συναγωνιστικού αναστολέα σε μεγαλύτερες συγκεντρώσεις αυτής. H β-alaCAM ευρέθηκε 4,6 φορές περισσότερο βιολογικά δραστική από την PhePheCAM και 14,3 βιολογικά ασθενέστερη από την μητρική ένωση. Σε αντίθεση με τη μητρική ένωση, η οποία δεν υφίσταται ισομερισμό, τόσο η β-alaCAM όσο και η PhePheCAM δίνουν, στην τελική ή δεύτερη χρονική φάση της αντίδρασης πουρομυκίνης, γένεση στο ισομερισμένο σύμπλοκο C*I. Αξιοσημείωτη είναι η παρατήρηση ότι ο σχηματισμός του ισομερισμένου συμπλόκου C*I λαμβάνει χώρα μέσω δύο κινητικών βημάτων στην περίπτωση της β-alaCAM, αλλά μέσω ενός μόνο κινητικού βήματος στην περίπτωση της PhePheCAM. Προτείνουμε, ως μοντέλο επεξηγηματικό του μηχανισμού βιολογικής δράσης… Advisors/Committee Members: Μάμος, Πέτρος, Kourelis, Theodore, Καλπαξής, Δημήτριος, Παπαϊωάννου, Διονύσιος, Μάμος, Πέτρος.

Subjects/Keywords: Χλωραμφαινικόλη; Πρωτεϊνοσύνθεση; Πουρομυκίνη; Βήτα αλανίνη χλωραμφαινικόλη; Φαινυλαλανίνη φαινυλαλανίνη χλωραμφαινικόλη; Κινητική; Αμινοακυλο ανάλογα; Πεπτιδικά ανάλογα; 615.329; Chloramphenicol; Proteinosynthesis; Puromycin; Beta alanine chloramphenicol; Phenylalanine phenylalanine chloramphenicol; Kinetics; Aminoacyl analogs; Peptidyl analogs

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Κουρέλης, �. (2009). Σύνθεση πεπτιδικών αναλόγων της χλωραμφαινικόλης και μελέτη της βιολογικής τους δραστικότητας. (Masters Thesis). University of Patras. Retrieved from http://nemertes.lis.upatras.gr/jspui/handle/10889/2460

Chicago Manual of Style (16^th Edition):

Κουρέλης, Θεόδωρος. “Σύνθεση πεπτιδικών αναλόγων της χλωραμφαινικόλης και μελέτη της βιολογικής τους δραστικότητας.” 2009. Masters Thesis, University of Patras. Accessed January 18, 2021. http://nemertes.lis.upatras.gr/jspui/handle/10889/2460.

MLA Handbook (7^th Edition):

Κουρέλης, Θεόδωρος. “Σύνθεση πεπτιδικών αναλόγων της χλωραμφαινικόλης και μελέτη της βιολογικής τους δραστικότητας.” 2009. Web. 18 Jan 2021.

Vancouver:

Κουρέλης �. Σύνθεση πεπτιδικών αναλόγων της χλωραμφαινικόλης και μελέτη της βιολογικής τους δραστικότητας. [Internet] [Masters thesis]. University of Patras; 2009. [cited 2021 Jan 18]. Available from: http://nemertes.lis.upatras.gr/jspui/handle/10889/2460.

Council of Science Editors:

Κουρέλης �. Σύνθεση πεπτιδικών αναλόγων της χλωραμφαινικόλης και μελέτη της βιολογικής τους δραστικότητας. [Masters Thesis]. University of Patras; 2009. Available from: http://nemertes.lis.upatras.gr/jspui/handle/10889/2460

Universidade do Rio Grande do Sul

20. Giugliani, Luciana. Hiperfenilalaninemia por deficiência de fenilalanina hidroxilase : identificação de indivíduos responsivos à administração de tetrahidrobiopterina por via oral.

Degree: 2009, Universidade do Rio Grande do Sul

URL: http://hdl.handle.net/10183/19023

►

Introdução: A Hiperfenilalaninemia por deficiência de fenilalanina hidroxilase (HPAPAH) é um erro inato do metabolismo no qual ocorre aumento dos níveis séricos de fenilalanina (Phe).… (more)

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Introdução: A Hiperfenilalaninemia por deficiência de fenilalanina hidroxilase (HPAPAH) é um erro inato do metabolismo no qual ocorre aumento dos níveis séricos de fenilalanina (Phe). Estudos recentes, realizados em várias populações, demonstraram que pacientes com HPA-PAH podem apresentar redução das concentrações plasmáticas de Phe mediante a administração oral de tetrahidrobiopterina (BH4). Objetivo: Identificar em uma amostra de pacientes brasileiros com HPA-PAH aqueles que são responsivos à administração de BH4 por via oral. Métodos: Para um paciente ser incluído no estudo, era necessário ter diagnóstico de HPA-PAH e idade igual ou superior a 7 anos, estar em tratamento dietético e apresentar nível de Phe igual ou superior a 6 mg/dL em todas as medidas realizadas no ano anterior à inclusão no estudo. No dia anterior à sobrecarga de BH4 (Dia 1), os pacientes foram submetidos a três coletas de sangue para mensuração dos níveis de Phe. No Dia 2, os pacientes receberam dose única de 20mg/Kg de BH4. As coletas de sangue foram, então, realizadas nos pontos de hora: 0, 4 e 8h (Dia 2) e 24h (Dias 3) após a ingestão do medicamento. Os níveis de Phe foram determinados através da espectrometria de massa in tandem. Foram utilizados dois critérios para definir a presença de responsividade ao BH4: Critério 1: redução 30% de Phe após 8h da administração do medicamento; Critério 2: redução 30% de Phe após 24h da administração do medicamento. Resultados: Dezoito pacientes foram incluídos no estudo, com mediana de idade de 14 anos, sendo 66,7% do sexo masculino. Onze apresentavam a forma clássica da doença e três a forma atípica. Três (forma clássica: 1, forma atípica: 2) e cinco (forma clássica: 2, forma atípica: 2 e forma não-definida: 1) pacientes foram considerados responsivos ao BH4 conforme critérios 1 e 2, respectivamente. Os níveis de Phe plasmáticos do dia anterior ao teste de sobrecarga não demonstraram variação nos pontos de hora (p=0,523). Entretanto, quando comparamos os níveis de Phe nos pontos de hora do dia pré e pós BH4, encontrou-se variação significativa entre eles (p=0,006). A análise da associação genótipo-fenótipo, para os pacientes com dados disponíveis (n=6) mostrou que a mesma é multifatorial. Conclusão: Nossos achados estão de acordo com a literatura, e indicaram que um número considerável de pacientes brasileiros com HPA-PAH poderá ser beneficiado com a administração oral de BH4.

Introduction: Hyperphenylalaninemia by phenylalanine hydroxylase deficiency (HPAPAH) is an inborn error of metabolism in which increased serum levels of phenylalanine (Phe) occur. Recent studies on several populations showed that patients with HPA-PAH can have their serum levels reduced when receiving oral tetrahydrobiopterin (BH4). Objective: to identify in a sample of Brazilian HPA-PAH the patients who are responsive to the oral administration of BH4. Methods: the following inclusion criteria were used: diagnosis of HPA-PAH, age 7 years, on dietary treatment and Phe levels 6 mg/dL in all tests performed one year prior to…

Advisors/Committee Members: Giugliani, Roberto.

Subjects/Keywords: Fenilcetonúrias; Phenylketonuria; PKU; Fenilalanina hidroxilase; Tetrahydrobiopterin (BH4); Biopterina; Responsiveness to BH4; Phenylalanine; Chaperones

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Giugliani, L. (2009). Hiperfenilalaninemia por deficiência de fenilalanina hidroxilase : identificação de indivíduos responsivos à administração de tetrahidrobiopterina por via oral. (Thesis). Universidade do Rio Grande do Sul. Retrieved from http://hdl.handle.net/10183/19023

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Giugliani, Luciana. “Hiperfenilalaninemia por deficiência de fenilalanina hidroxilase : identificação de indivíduos responsivos à administração de tetrahidrobiopterina por via oral.” 2009. Thesis, Universidade do Rio Grande do Sul. Accessed January 18, 2021. http://hdl.handle.net/10183/19023.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Giugliani, Luciana. “Hiperfenilalaninemia por deficiência de fenilalanina hidroxilase : identificação de indivíduos responsivos à administração de tetrahidrobiopterina por via oral.” 2009. Web. 18 Jan 2021.

Vancouver:

Giugliani L. Hiperfenilalaninemia por deficiência de fenilalanina hidroxilase : identificação de indivíduos responsivos à administração de tetrahidrobiopterina por via oral. [Internet] [Thesis]. Universidade do Rio Grande do Sul; 2009. [cited 2021 Jan 18]. Available from: http://hdl.handle.net/10183/19023.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Giugliani L. Hiperfenilalaninemia por deficiência de fenilalanina hidroxilase : identificação de indivíduos responsivos à administração de tetrahidrobiopterina por via oral. [Thesis]. Universidade do Rio Grande do Sul; 2009. Available from: http://hdl.handle.net/10183/19023

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

21. Simone Maria Menegatti de Oliveira. AvaliaÃÃo da produÃÃo biotecnolÃgica de 2-feniletanol em resÃduo lÃquido de fecularia.

Degree: 2010, Universidade Estadual do Oeste do Parana

URL: http://tede.unioeste.br/tede//tde_busca/arquivo.php?codArquivo=665

►

2-phenylethanol is the most used fragrance in the food, cosmetics and fragrance industry. It is mainly produced by chemical process, but with many impurities. The… (more)

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2-phenylethanol is the most used fragrance in the food, cosmetics and fragrance industry. It is mainly produced by chemical process, but with many impurities. The biotechnological process of production is feasible and is being studied with various microrganisms, among them, Geotrichum fragrans, Saccharomyces cerevisiae and Kluyveromyces marxianus. The use of cassava wastewater as substrate in this process makes it less expensive and reduce environmental pollution caused by it. The objective or this work was to evaluate the production of 2-phenylethanol in the cultivation of Geotrichum fragrans, Saccharomyces cerevisiae and Kluyveromyces marxianus in cassava wastewater, analyzing the effect of two carbon sources on production of the aroma, besides increasing the production of 2 - phenylethanol to the greater efficiency of conversion carbon source/2-feniletanol, varying concentrations of carbon source and phenylalanine at cassava wastewater. For this, the three microrganisms mentioned were cultured in 50mL cassava wastewater plus 50g.L-1 of glucose and 3g.L-1 of L-phenylalanine in sterile Erlenmeyer flasks, on shaker at 150 rpm, 24 Â C for 120 hours, to check the time of peak production of 2-phenylethanol and what microrganism largest producer. It was then checked the production of aroma with 50g.L-1 of glucose and fructose, while maintaining the same amount of L-phenylalanine. And finally, by fatorial design, were tested concentrations of glucose and L-phenylalanine, in order to obtain higher yield and bioconversion of 2-phenylethanol. The experimental model was validated. It was analyzed in the pH, COD, reducing sugars, L-phenylalanine and 2-phenylethanol. In the precipitate was quantified indirectly as biomass, the Volatile Suspended Solids. The results showed that S. cerevisiae was the best producer of 2-phenylethanol in the effluent of cassava starch, obtaining 0.74 g.L-1, followed by K. marxianus, with 0.19 g.L-1 and finally by G. fragrans with 0.08 g.L-1. Glucose was the best carbon source for obtention of 2- phenylethanol for S. cerevisiae and K. marxianus. There was no significant difference between the production of 2-phenylethanol using glucose or fructose for G. fragrans. The best combination of glucose and L-phenylalanine for production of 2-phenylethanol, was 20,0 and 5.5 g.L-1 respectively, obtaining production of 1.33 g.L-1of aroma in the validation.

O 2-feniletanol Ã a fragrÃncia mais utilizada na indÃstria de alimentos, cosmÃtica e de perfumes. Ã produzido principalmente por processo quÃmico, porÃm com muitas impurezas. O processo biotecnolÃgico de produÃÃo Ã viÃvel e estÃ sendo estudado com vÃrios microrganismos, entre eles, o Geotrichum fragrans (GF), Saccharomyces cerevisiae (SC) e Kluyveromyces marxianus (KM). O uso de resÃduo lÃquido de fecularia como substrato neste processo torna-o menos dispendioso, alÃm de reduzir a poluiÃÃo ambiental causada pelo mesmo. O objetivo deste trabalho foi avaliar a produÃÃo de 2-feniletanol no cultivo de GF, SC e KM em resÃduo lÃquido de fecularia, analisando o…

Advisors/Committee Members: Simone Damasceno Gomes.

Subjects/Keywords: aroma; cultivo aerÃbio; L-fenilalanina; aroma; aerobic cultivation; L-phenylalanine; ENGENHARIA AGRICOLA

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Oliveira, S. M. M. d. (2010). AvaliaÃÃo da produÃÃo biotecnolÃgica de 2-feniletanol em resÃduo lÃquido de fecularia. (Thesis). Universidade Estadual do Oeste do Parana. Retrieved from http://tede.unioeste.br/tede//tde_busca/arquivo.php?codArquivo=665

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Oliveira, Simone Maria Menegatti de. “AvaliaÃÃo da produÃÃo biotecnolÃgica de 2-feniletanol em resÃduo lÃquido de fecularia.” 2010. Thesis, Universidade Estadual do Oeste do Parana. Accessed January 18, 2021. http://tede.unioeste.br/tede//tde_busca/arquivo.php?codArquivo=665.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Oliveira, Simone Maria Menegatti de. “AvaliaÃÃo da produÃÃo biotecnolÃgica de 2-feniletanol em resÃduo lÃquido de fecularia.” 2010. Web. 18 Jan 2021.

Vancouver:

Oliveira SMMd. AvaliaÃÃo da produÃÃo biotecnolÃgica de 2-feniletanol em resÃduo lÃquido de fecularia. [Internet] [Thesis]. Universidade Estadual do Oeste do Parana; 2010. [cited 2021 Jan 18]. Available from: http://tede.unioeste.br/tede//tde_busca/arquivo.php?codArquivo=665.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Oliveira SMMd. AvaliaÃÃo da produÃÃo biotecnolÃgica de 2-feniletanol em resÃduo lÃquido de fecularia. [Thesis]. Universidade Estadual do Oeste do Parana; 2010. Available from: http://tede.unioeste.br/tede//tde_busca/arquivo.php?codArquivo=665

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Texas A&M University

22. Tuley, Alfred. Expanding the Genetic Code to Probe Biological Systems.

Degree: PhD, Chemistry, 2015, Texas A&M University

URL: http://hdl.handle.net/1969.1/187469

► The chemical modification of proteins has been a longstanding interest in the scientific community. In addition to the natural modifications necessary for life to function,… (more)

▼ The chemical modification of proteins has been a longstanding interest in the scientific community. In addition to the natural modifications necessary for life to function, unnatural covalent modifications are particularly useful because they facilitate research efforts that require the precise manipulation of protein, including the installation of fluorescent labels, post-translational modification mimics, and affinity reagents. Historically, appending such modifications onto proteins was achieved by generating covalent adducts onto one of the twenty canonical amino acids. However, such modifications are not site-selective, and may interfere with the native function of the modified protein. Genetic code expansion can overcome the limitations inherent to canonical amino acid modification, especially when bioorthogonal functional groups are incorporated. Using orthogonal aminoacyl-tRNA synthetase-tRNA pairs, one can reliably obtain homogenous samples of modified protein in a site-selective manner. In order to fully understand the steric requirements of a rationally designed pyrrolysyl-tRNA synthetase, several large meta-substituted phenylalanine derivatives were synthesized and incorporated into superfolder green fluorescent protein using this synthetase. All synthesized substrates were incorporated, albeit with differing incorporation efficiencies. Moreover, this synthetase was found to incorporate 3-formyl-phenylalanine, an aldehyde-based amino acid that can be directly installed onto proteins. Prior to this work, only indirect post-translational approaches could install aldehydes, and these methodologies were limited as to where the modification could occur. Aldehyde labeling occurs rapidly at neutral pH, and peptide cyclization has been accomplished using the aldehyde to form a thiazolidine linkage with an N-terminal cysteine, further demonstrating the rich chemistry available to aldehydes. Finally, efforts to optimize azidophenylalanine bioconjugation led to a kinetic investigation of copper-catalyzed click chemistry, which led to a revision of the currently accepted mechanism; it is proposed that copper-catalyzed click chemistry requires two separate copper-chelating events, with one equivalent of copper binding to azide, and another equivalent of copper binding to alkyne. The work presented herein demonstrates that phenylalanine derivatives are useful substrates for probing and manipulating biological systems, as well as providing opportunities for discovery in chemical biology. Advisors/Committee Members: Liu, Wenshe (advisor), Begley, Tadhg P (committee member), Raushel, Frank M (committee member), Porter, Weston (committee member).

Subjects/Keywords: Genetic Code Expansion; Pyrrolysine; Phenylalanine; Non-Canonical Amino Acids; Aldehyde; Click Chemistry; Phage Display

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Tuley, A. (2015). Expanding the Genetic Code to Probe Biological Systems. (Doctoral Dissertation). Texas A&M University. Retrieved from http://hdl.handle.net/1969.1/187469

Chicago Manual of Style (16^th Edition):

Tuley, Alfred. “Expanding the Genetic Code to Probe Biological Systems.” 2015. Doctoral Dissertation, Texas A&M University. Accessed January 18, 2021. http://hdl.handle.net/1969.1/187469.

MLA Handbook (7^th Edition):

Tuley, Alfred. “Expanding the Genetic Code to Probe Biological Systems.” 2015. Web. 18 Jan 2021.

Vancouver:

Tuley A. Expanding the Genetic Code to Probe Biological Systems. [Internet] [Doctoral dissertation]. Texas A&M University; 2015. [cited 2021 Jan 18]. Available from: http://hdl.handle.net/1969.1/187469.

Council of Science Editors:

Tuley A. Expanding the Genetic Code to Probe Biological Systems. [Doctoral Dissertation]. Texas A&M University; 2015. Available from: http://hdl.handle.net/1969.1/187469

Washington State University

23. [No author]. Arabidopsis Arogenate Dehydratases: Influence on Aromatic Amino Acid and Downstream Phenylpropanoid Biosynthesis .

Degree: 2011, Washington State University

URL: http://hdl.handle.net/2376/3526

► Arogenate dehydratases (ADTs) perform the final step of phenylalanine (Phe) biosynthesis in plants. As one of twenty protein-coding amino acids, Phe is essential in all… (more)

▼ Arogenate dehydratases (ADTs) perform the final step of phenylalanine (Phe) biosynthesis in plants. As one of twenty protein-coding amino acids, Phe is essential in all living organisms, and in plants it is also a precursor to thousands of different phenylpropanoid that have wide-ranging functions in planta. Undoubtedly, the most important of these (in terms of vascular plant evolution and survival), is the polymer lignin, which provides additional strength to cell walls, enabling plants to grow upright, and retain water in a desiccating environment. The goal of this study was to understand the role of ADTs in the chloroplast-localized pathway upstream of Phe, particularly regarding possible influences on downstream cytosol/cell wall localized phenylpropanoid biosynthesis. All six ADT isoenzymes from Arabidopsis thaliana were first individually biochemically characterized; this confirmed Arabidopsis ADTs preferentially utilized arogenate as a substrate. Subsequent studies focused on characterization of ADTs in planta. Accordingly, Arabidopsis knockout (KO) lines were obtained for five/six ADTs and were crossed to obtain double, triple and quadruple KO lines. These KOs were assessed for potential reductions in free Phe levels and lignin contents over the course of growth and development. Results of these analyses identified vast reductions in lignin levels in certain ADT KO lines (up to 70% in a quadruple KO compared to WT). Amino acid analyses identified diminished Phe levels in the same lines with reduced lignin contents, but apparently only in lignified tissue during times when lignin biosynthesis and deposition are most active. Together, these analyses revealed that the isoenzyme ADT5 is prominently involved in the synthesis of Phe as a precursor for lignin. Finally, an analysis of ADT expression/localization using the â-glucuronidase (GUS) reporter gene, found that ADT5 is mainly expressed in the lignifying regions of the stem, further supporting the above findings. Together, these studies showed that individual ADTs in Arabidopsis have distinct roles to accommodate the many different requirements for Phe in different cell types and tissues. Advisors/Committee Members: Lewis, Norman G (advisor).

Subjects/Keywords: Molecular biology; Biochemistry; Plant biology; Arabidopsis; Arogenate dehydratase; Lignin; Phenylalanine; Phenylpropanoid biosynthesis

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APA (6^th Edition):

author], [. (2011). Arabidopsis Arogenate Dehydratases: Influence on Aromatic Amino Acid and Downstream Phenylpropanoid Biosynthesis . (Thesis). Washington State University. Retrieved from http://hdl.handle.net/2376/3526

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

author], [No. “Arabidopsis Arogenate Dehydratases: Influence on Aromatic Amino Acid and Downstream Phenylpropanoid Biosynthesis .” 2011. Thesis, Washington State University. Accessed January 18, 2021. http://hdl.handle.net/2376/3526.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

author], [No. “Arabidopsis Arogenate Dehydratases: Influence on Aromatic Amino Acid and Downstream Phenylpropanoid Biosynthesis .” 2011. Web. 18 Jan 2021.

Vancouver:

author] [. Arabidopsis Arogenate Dehydratases: Influence on Aromatic Amino Acid and Downstream Phenylpropanoid Biosynthesis . [Internet] [Thesis]. Washington State University; 2011. [cited 2021 Jan 18]. Available from: http://hdl.handle.net/2376/3526.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

author] [. Arabidopsis Arogenate Dehydratases: Influence on Aromatic Amino Acid and Downstream Phenylpropanoid Biosynthesis . [Thesis]. Washington State University; 2011. Available from: http://hdl.handle.net/2376/3526

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Universidade do Rio Grande do Sul

24. Nalin, Tatiéle. Hiperfenilalaninemia por deficiência de fenilalanina hidroxilase : avaliação da responsividade ao BH4 em pacientes acompanhados no Serviço de Genética Médica do HCPA e que apresentam controle metabólico adequado.

Degree: 2011, Universidade do Rio Grande do Sul

URL: http://hdl.handle.net/10183/31887

► Introdução: Estudos recentes, utilizando vários protocolos, têm demonstrado que pacientes com Hiperfenilalaninemia por deficiência de fenilalanina hidroxilase (HPA-PAH) podem apresentar redução das concentrações plasmáticas de… (more)

▼ Introdução: Estudos recentes, utilizando vários protocolos, têm demonstrado que pacientes com Hiperfenilalaninemia por deficiência de fenilalanina hidroxilase (HPA-PAH) podem apresentar redução das concentrações plasmáticas de fenilalanina (Phe) mediante a administração de tetrahidrobiopterina (BH4). Objetivo: Determinar, em uma amostra de pacientes brasileiros com HPA-PAH, a responsividade à administração de dose única de BH4 por meio de um protocolo incluindo o teste de sobrecarga simples de Phe e o teste combinado de sobrecarga de Phe+BH4. Métodos: Foram incluídos no estudo pacientes com idade ≥ 4 anos, em tratamento dietético e que possuíam mediana de Phe plasmática inferior a 10mg/dL no ano anterior à inclusão. Foram realizados teste de sobrecarga simples de Phe, utilizando 100mg/kg de L-Phe (Teste 1) e teste combinado de Phe+BH4, utilizando 100mg/kg de L-Phe e 20mg/kg de BH4 (Teste 2), com intervalo de uma semana entre ambos. A ingestão do BH4 ocorreu após três horas da ingestão da Phe. Foram realizadas coletas de sangue no ponto basal e após três, 11 e 27h da ingestão de Phe (T0, T1, T2 e T3 dos testes 1 e 2, respectivamente). Para ser considerado responsivo, o paciente deveria apresentar evidência de redução dos níveis de Phe associada à administração do BH4 de acordo com pelo menos um dos seguintes critérios: critério A – análise das diferenças, em percentual, dos valores de Phe nos pontos T1 e T2 dos Testes 1 e 2; critério B – análise das diferenças, em percentual, dos valores de Phe nos pontos T1 e T3 dos Testes 1 e 2 e critério C – análise da diferença, em percentual, das áreas abaixo da curva de Phe entre os Testes 1 e 2. A classificação de responsividade foi também comparada com quatro critérios adicionais: critério D – análise da diferença, em percentual, dos valores de Phe nos pontos T1 e T2 do Teste 2; critério E – análise da diferença, em percentual, dos valores de Phe nos pontos T1 e T3 do Teste 2; cinco pacientes participaram de estudo anterior do grupo e foram também classificados através do critério F – análise da diferença, em percentual, dos valores de Phe após 8h da sobrecarga simples com BH4 e do critério G – análise da diferença, em percentual, dos valores de Phe após 24h da sobrecarga simples com BH4. Para todos os critérios foi utilizado como ponte de corte redução ≥ 30%. Resultados: Dezoito pacientes, com mediana de idade de 12 anos, participaram do estudo. Dez pacientes apresentavam a forma Leve de HPA-PAH e oito a forma Clássica. Seis pacientes foram considerados responsivos de acordo com os critérios adotados (Clássica: 2; Leve: 4). Houve concordância de responsividade entre os critérios A e B em relação ao C (Índice Kappa=0,557; p=0,017). Dos pacientes com genótipo disponível (n=16), seis possuíam dados de responsividade ao BH4 descritos na literatura, que foram concordantes com os encontrados no presente estudo. Conclusão: Dados relativos à responsividade, tipo de HPA-PAH e genotipagem estão de acordo com descrito na literatura. Haja vista a diferença de responsividade dos… Advisors/Committee Members: Schwartz, Ida Vanessa Doederlein.

Subjects/Keywords: Fenilcetonúrias; Inborn errors of metabolism; Hyperphenylalaninemia; Fenilalanina hidroxilase; Genética médica; Phenylketonuria; Phenylalanine; Tetrahydrobiopterin

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Nalin, T. (2011). Hiperfenilalaninemia por deficiência de fenilalanina hidroxilase : avaliação da responsividade ao BH4 em pacientes acompanhados no Serviço de Genética Médica do HCPA e que apresentam controle metabólico adequado. (Thesis). Universidade do Rio Grande do Sul. Retrieved from http://hdl.handle.net/10183/31887

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Nalin, Tatiéle. “Hiperfenilalaninemia por deficiência de fenilalanina hidroxilase : avaliação da responsividade ao BH4 em pacientes acompanhados no Serviço de Genética Médica do HCPA e que apresentam controle metabólico adequado.” 2011. Thesis, Universidade do Rio Grande do Sul. Accessed January 18, 2021. http://hdl.handle.net/10183/31887.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Nalin, Tatiéle. “Hiperfenilalaninemia por deficiência de fenilalanina hidroxilase : avaliação da responsividade ao BH4 em pacientes acompanhados no Serviço de Genética Médica do HCPA e que apresentam controle metabólico adequado.” 2011. Web. 18 Jan 2021.

Vancouver:

Nalin T. Hiperfenilalaninemia por deficiência de fenilalanina hidroxilase : avaliação da responsividade ao BH4 em pacientes acompanhados no Serviço de Genética Médica do HCPA e que apresentam controle metabólico adequado. [Internet] [Thesis]. Universidade do Rio Grande do Sul; 2011. [cited 2021 Jan 18]. Available from: http://hdl.handle.net/10183/31887.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Nalin T. Hiperfenilalaninemia por deficiência de fenilalanina hidroxilase : avaliação da responsividade ao BH4 em pacientes acompanhados no Serviço de Genética Médica do HCPA e que apresentam controle metabólico adequado. [Thesis]. Universidade do Rio Grande do Sul; 2011. Available from: http://hdl.handle.net/10183/31887

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Queensland University of Technology

25. Sharman, Rachael Rebecca. Neuropsychological development in children with early and continuously treated phenylketonuria : association with biochemical markers.

Degree: 2011, Queensland University of Technology

URL: https://eprints.qut.edu.au/46646/

► PKU is a genetically inherited inborn error of metabolism caused by a deficiency of the enzyme phenylalanine hydroxylase. The failure of this enzyme causes incomplete… (more)

▼ PKU is a genetically inherited inborn error of metabolism caused by a deficiency of the enzyme phenylalanine hydroxylase. The failure of this enzyme causes incomplete metabolism of protein ingested in the diet, specifically the conversion of one amino acid, phenylalanine, to tyrosine, which is a precursor to the neurotransmitter dopamine. Rising levels of phenylalanine is toxic to the developing brain, disrupting the formation of white matter tracts. The impact of tyrosine deficiency is not as well understood, but is hypothesized to lead to a low dopamine environment for the developing brain. Detection in the newborn period and continuous treatment (a low protein phe-restricted diet supplemented with phenylalanine-free protein formulas) has resulted in children with early and continuously treated PKU now developing normal I.Q. However, deficits in executive function (EF) are common, leading to a rate of Attention Deficit Hyperactivity Disorder (ADHD) up to five times the norm. EF worsens with exposure to higher phenylalanine levels, however recent research has demonstrated that a high phenylalanine to tyrosine ratio (phenylalanine:tyrosine ratio), which is hypothesised to lead to poorer dopamine function, has a more negative impact on EF than phenylalanine levels alone. Research and treatment of PKU is currently phenylalanine-focused, with little investigation of the impact of tyrosine on neuropsychological development. There is no current consensus as to the veracity of tyrosine monitoring or treatment in this population. Further, the research agenda in this population has demonstrated a primary focus on EF impairment alone, even though there may be additional neuropsychological skills compromised (e.g., mood, visuospatial deficits). The aim of this PhD research was to identify residual neuropsychological deficits in a cohort of children with early and continuously treated phenylketonuria, at two time points in development (early childhood and early adolescence), separated by eight years. In addition, this research sought to determine which biochemical markers were associated with neuropsychological impairments. A clinical practice survey was also undertaken to ascertain the current level of monitoring/treatment of tyrosine in this population. Thirteen children with early and continuously treated PKU were tested at mean age 5.9 years and again at mean age 13.95 years on several neuropsychological measures. Four children with hyperphenylalaninemia (a milder version of PKU) were also tested at both time points and provide a comparison group in analyses. Associations between neuropsychological function and biochemical markers were analysed. A between groups analysis in adolescence was also conducted (children with PKU compared to their siblings) on parent report measures of EF and mood. Minor EF impairments were evident in the PKU group by age 6 years and these persisted into adolescence. Life-long exposure to high phenylalanine:tyrosine ratio and/or low tyrosine independent of phenylalanine…

Subjects/Keywords: phenylketonuria; phenylalanine; tyrosine; dopamine; executive function; working memory; phenylalanine:tyrosine ratio; depression; practice survey

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APA (6^th Edition):

Sharman, R. R. (2011). Neuropsychological development in children with early and continuously treated phenylketonuria : association with biochemical markers. (Thesis). Queensland University of Technology. Retrieved from https://eprints.qut.edu.au/46646/

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Sharman, Rachael Rebecca. “Neuropsychological development in children with early and continuously treated phenylketonuria : association with biochemical markers.” 2011. Thesis, Queensland University of Technology. Accessed January 18, 2021. https://eprints.qut.edu.au/46646/.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Sharman, Rachael Rebecca. “Neuropsychological development in children with early and continuously treated phenylketonuria : association with biochemical markers.” 2011. Web. 18 Jan 2021.

Vancouver:

Sharman RR. Neuropsychological development in children with early and continuously treated phenylketonuria : association with biochemical markers. [Internet] [Thesis]. Queensland University of Technology; 2011. [cited 2021 Jan 18]. Available from: https://eprints.qut.edu.au/46646/.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Sharman RR. Neuropsychological development in children with early and continuously treated phenylketonuria : association with biochemical markers. [Thesis]. Queensland University of Technology; 2011. Available from: https://eprints.qut.edu.au/46646/

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Adelaide

26. Lines, David R. The effect of phenylalanine analogues on the transport of metabolism of phenylalanine : with special reference to the possible use in phenylketonuria / by David Robin Lines.

Degree: 1984, University of Adelaide

URL: http://hdl.handle.net/2440/38355

Subjects/Keywords: 616.3995061 19; Phenylalanine Metabolism.; Phenylketonuria Chemotherapy.

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APA (6^th Edition):

Lines, D. R. (1984). The effect of phenylalanine analogues on the transport of metabolism of phenylalanine : with special reference to the possible use in phenylketonuria / by David Robin Lines. (Thesis). University of Adelaide. Retrieved from http://hdl.handle.net/2440/38355

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Lines, David R. “The effect of phenylalanine analogues on the transport of metabolism of phenylalanine : with special reference to the possible use in phenylketonuria / by David Robin Lines.” 1984. Thesis, University of Adelaide. Accessed January 18, 2021. http://hdl.handle.net/2440/38355.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Lines, David R. “The effect of phenylalanine analogues on the transport of metabolism of phenylalanine : with special reference to the possible use in phenylketonuria / by David Robin Lines.” 1984. Web. 18 Jan 2021.

Vancouver:

Lines DR. The effect of phenylalanine analogues on the transport of metabolism of phenylalanine : with special reference to the possible use in phenylketonuria / by David Robin Lines. [Internet] [Thesis]. University of Adelaide; 1984. [cited 2021 Jan 18]. Available from: http://hdl.handle.net/2440/38355.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Lines DR. The effect of phenylalanine analogues on the transport of metabolism of phenylalanine : with special reference to the possible use in phenylketonuria / by David Robin Lines. [Thesis]. University of Adelaide; 1984. Available from: http://hdl.handle.net/2440/38355

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Western Ontario

27. Van Brenk, Jordan B. Express Yourself: Analysis of the mRNA Expression Patterns of the AROGENATE DEHYDRATASE Family in Arabidopsis thaliana.

Degree: 2020, University of Western Ontario

URL: https://ir.lib.uwo.ca/etd/7046

► Phenylalanine (Phe) is an essential aromatic amino acid synthesized by plants and the final reaction in its biosynthesis is catalyzed by AROGENATE DEHYDRATASEs (ADTs). In… (more)

Subjects/Keywords: phenylalanine; arogenate dehydratase; transcriptional expression; core promoter; proximal promoter; cis-regulatory motif; gene family; Biology

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APA (6^th Edition):

Van Brenk, J. B. (2020). Express Yourself: Analysis of the mRNA Expression Patterns of the AROGENATE DEHYDRATASE Family in Arabidopsis thaliana. (Thesis). University of Western Ontario. Retrieved from https://ir.lib.uwo.ca/etd/7046

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Van Brenk, Jordan B. “Express Yourself: Analysis of the mRNA Expression Patterns of the AROGENATE DEHYDRATASE Family in Arabidopsis thaliana.” 2020. Thesis, University of Western Ontario. Accessed January 18, 2021. https://ir.lib.uwo.ca/etd/7046.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Van Brenk, Jordan B. “Express Yourself: Analysis of the mRNA Expression Patterns of the AROGENATE DEHYDRATASE Family in Arabidopsis thaliana.” 2020. Web. 18 Jan 2021.

Vancouver:

Van Brenk JB. Express Yourself: Analysis of the mRNA Expression Patterns of the AROGENATE DEHYDRATASE Family in Arabidopsis thaliana. [Internet] [Thesis]. University of Western Ontario; 2020. [cited 2021 Jan 18]. Available from: https://ir.lib.uwo.ca/etd/7046.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Van Brenk JB. Express Yourself: Analysis of the mRNA Expression Patterns of the AROGENATE DEHYDRATASE Family in Arabidopsis thaliana. [Thesis]. University of Western Ontario; 2020. Available from: https://ir.lib.uwo.ca/etd/7046

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Missouri University of Science and Technology

28. Pringle, Erin Kathleen. The identification and characterization of phenylalanine ammonia-lyase gene family members in glycine max.

Degree: M.S. in Applied and Environmental Biology, Applied and Environmental Biology, Missouri University of Science and Technology

URL: https://scholarsmine.mst.edu/masters_theses/5017

► "Gene families are collections of genes with similar functions. Studying gene families is important for understanding the evolution of genes and manipulating genes. Phenylalanine ammonia-lyase… (more)

Subjects/Keywords: Phenylalanine ammonia-lyase; Biology; Environmental Sciences

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APA (6^th Edition):

Pringle, E. K. (n.d.). The identification and characterization of phenylalanine ammonia-lyase gene family members in glycine max. (Masters Thesis). Missouri University of Science and Technology. Retrieved from https://scholarsmine.mst.edu/masters_theses/5017

Note: this citation may be lacking information needed for this citation format:
No year of publication.

Chicago Manual of Style (16^th Edition):

Pringle, Erin Kathleen. “The identification and characterization of phenylalanine ammonia-lyase gene family members in glycine max.” Masters Thesis, Missouri University of Science and Technology. Accessed January 18, 2021. https://scholarsmine.mst.edu/masters_theses/5017.

Note: this citation may be lacking information needed for this citation format:
No year of publication.

MLA Handbook (7^th Edition):

Pringle, Erin Kathleen. “The identification and characterization of phenylalanine ammonia-lyase gene family members in glycine max.” Web. 18 Jan 2021.

Note: this citation may be lacking information needed for this citation format:
No year of publication.

Vancouver:

Pringle EK. The identification and characterization of phenylalanine ammonia-lyase gene family members in glycine max. [Internet] [Masters thesis]. Missouri University of Science and Technology; [cited 2021 Jan 18]. Available from: https://scholarsmine.mst.edu/masters_theses/5017.

Note: this citation may be lacking information needed for this citation format:
No year of publication.

Council of Science Editors:

Pringle EK. The identification and characterization of phenylalanine ammonia-lyase gene family members in glycine max. [Masters Thesis]. Missouri University of Science and Technology; Available from: https://scholarsmine.mst.edu/masters_theses/5017

Note: this citation may be lacking information needed for this citation format:
No year of publication.

University of Colorado

29. Griffith, Elizabeth Campbell. Chemical and Physical Changes Unique in and on Water: Implications for Life and Its Origins.

Degree: PhD, Chemistry & Biochemistry, 2014, University of Colorado

URL: https://scholar.colorado.edu/chem_gradetds/114

► In this thesis, the unique environment provided by water and its surface is exploited to aid in answering some of the questions surrounding life… (more)

▼ In this thesis, the unique environment provided by water and its surface is exploited to aid in answering some of the questions surrounding life and its origins. First, the photochemistry of pyruvic acid, a molecule important in modern metabolism and present throughout the modern atmosphere, is explored in the gas phase, atmospheric aerosol particles, and into aqueous solution, illustrating the sensitivity of chemical reactions to environmental conditions. Then, this chemistry is extended to the photochemistry of a single-tailed surfactant of similar chemical functionality, 2-oxooctanoic acid, producing a double-tailed surfactant abiotically followed by spontaneous self-assembly into stable vesicles. This yields a prebiotically plausible synthesis of a primitive enclosure and provides a contribution to the evolution of early membranes in the origin of life. Further, the water surface is shown to be an advantageous environment for unique chemistry beyond what is available in bulk aqueous solution by selectively concentrating, aligning, and altering the ionization state of useful reactants. In this thesis the ionization state of L-phenylalanine, a natural amino acid, is examined in situ spectroscopically in the surface region, illustrating the change in ionization state at the surface compared with bulk aqueous solution. Further, abiotic peptide bond formation is demonstrated exclusively at the water surface and observed in situ. Condensation reactions such as peptide bond formation are unlikely in bulk aqueous solution on both thermodynamic and kinetic grounds, yet are necessary in many biopolymers essential to modern life. The water surface provides a unique environment for this chemistry. Finally, mixed surfactant films of differing hydrophobic structure at the water surface are investigated in this thesis. The natural environment is quite complex, stemming from the multifaceted emissions from both biogenic and anthropogenic sources. The specific interactions of water-soluble aromatics (L-phenylalanine, benzoic acid, benzaldehyde) on a stearic acid monolayer film at the air - water interface are studied here relevant to the surfaces of atmospheric aerosol particles and their potential influence on climate. Finally, the same methods are used to explore the interactions of a perturbant (L-phenylalanine) on a model cell membrane (DPPC), contributing to an understanding of the mechanism of disease promoted by the presence of aromatics. Advisors/Committee Members: Veronica Vaida, Joel D. Eaves, Carol E. Cleland, Veronica M. Bierbaum, Tom R. Cech.

Subjects/Keywords: water surface; pyruvic acid; 2-oxooctanoic acid; L-phenylalanine; peptide bond; surfactant film; Physical Chemistry

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Griffith, E. C. (2014). Chemical and Physical Changes Unique in and on Water: Implications for Life and Its Origins. (Doctoral Dissertation). University of Colorado. Retrieved from https://scholar.colorado.edu/chem_gradetds/114

Chicago Manual of Style (16^th Edition):

Griffith, Elizabeth Campbell. “Chemical and Physical Changes Unique in and on Water: Implications for Life and Its Origins.” 2014. Doctoral Dissertation, University of Colorado. Accessed January 18, 2021. https://scholar.colorado.edu/chem_gradetds/114.

MLA Handbook (7^th Edition):

Griffith, Elizabeth Campbell. “Chemical and Physical Changes Unique in and on Water: Implications for Life and Its Origins.” 2014. Web. 18 Jan 2021.

Vancouver:

Griffith EC. Chemical and Physical Changes Unique in and on Water: Implications for Life and Its Origins. [Internet] [Doctoral dissertation]. University of Colorado; 2014. [cited 2021 Jan 18]. Available from: https://scholar.colorado.edu/chem_gradetds/114.

Council of Science Editors:

Griffith EC. Chemical and Physical Changes Unique in and on Water: Implications for Life and Its Origins. [Doctoral Dissertation]. University of Colorado; 2014. Available from: https://scholar.colorado.edu/chem_gradetds/114

University of Western Ontario

30. Cornelius, Emily J. Characterization of six Arabidopsis AROGENATE DEHYDRATASE promoters.

Degree: 2017, University of Western Ontario

URL: https://ir.lib.uwo.ca/etd/5081

► Phenylalanine is an important aromatic amino acid synthesized by higher plants, and is a major component of numerous specialized metabolites including structural components, pigments, and… (more)

Subjects/Keywords: Phenylalanine; specialized metabolism; arogenate dehydratase; cis regulatory motif; co-expression; transcriptional regulation; Biology

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Cornelius, E. J. (2017). Characterization of six Arabidopsis AROGENATE DEHYDRATASE promoters. (Thesis). University of Western Ontario. Retrieved from https://ir.lib.uwo.ca/etd/5081

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Cornelius, Emily J. “Characterization of six Arabidopsis AROGENATE DEHYDRATASE promoters.” 2017. Thesis, University of Western Ontario. Accessed January 18, 2021. https://ir.lib.uwo.ca/etd/5081.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Cornelius, Emily J. “Characterization of six Arabidopsis AROGENATE DEHYDRATASE promoters.” 2017. Web. 18 Jan 2021.

Vancouver:

Cornelius EJ. Characterization of six Arabidopsis AROGENATE DEHYDRATASE promoters. [Internet] [Thesis]. University of Western Ontario; 2017. [cited 2021 Jan 18]. Available from: https://ir.lib.uwo.ca/etd/5081.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Cornelius EJ. Characterization of six Arabidopsis AROGENATE DEHYDRATASE promoters. [Thesis]. University of Western Ontario; 2017. Available from: https://ir.lib.uwo.ca/etd/5081

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Open Access Theses and Dissertations