subject:(Peroxynitrite)

NSYSU

1. Tseng, Hsiu- Ting. Peritonitis-induced Peroxynitrite Production of Hematopoietic Cells and Lung Damage Depends on the JNK Signaling Pathway.

Degree: Master, Biological Sciences, 2008, NSYSU

URL: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0804108-215703

► Abdominal sepsis is a common, life-threatening condition in the critically ill patients. The c-Jun N-terminal kinase (JNK) is known as a stress-activated protein kinase, in… (more)

Subjects/Keywords: JNK; Peroxynitrite

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Tseng, H. T. (2008). Peritonitis-induced Peroxynitrite Production of Hematopoietic Cells and Lung Damage Depends on the JNK Signaling Pathway. (Thesis). NSYSU. Retrieved from http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0804108-215703

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Tseng, Hsiu- Ting. “Peritonitis-induced Peroxynitrite Production of Hematopoietic Cells and Lung Damage Depends on the JNK Signaling Pathway.” 2008. Thesis, NSYSU. Accessed January 18, 2021. http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0804108-215703.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Tseng, Hsiu- Ting. “Peritonitis-induced Peroxynitrite Production of Hematopoietic Cells and Lung Damage Depends on the JNK Signaling Pathway.” 2008. Web. 18 Jan 2021.

Vancouver:

Tseng HT. Peritonitis-induced Peroxynitrite Production of Hematopoietic Cells and Lung Damage Depends on the JNK Signaling Pathway. [Internet] [Thesis]. NSYSU; 2008. [cited 2021 Jan 18]. Available from: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0804108-215703.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Tseng HT. Peritonitis-induced Peroxynitrite Production of Hematopoietic Cells and Lung Damage Depends on the JNK Signaling Pathway. [Thesis]. NSYSU; 2008. Available from: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0804108-215703

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

2. Khan, Asad. Studies on experimentally produced Antibodies against peroxynitrite Modified histones; -.

Degree: Biochemistry, 2008, Aligarh Muslim University

URL: http://shodhganga.inflibnet.ac.in/handle/10603/17961

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Reactive nitrogen and oxygen species are produced under physiological newlineconditions. However, excess of these radicals may damage cellular lipids, proteins and newlinenucleic acids. These reactive… (more)

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Reactive nitrogen and oxygen species are produced under physiological newlineconditions. However, excess of these radicals may damage cellular lipids, proteins and newlinenucleic acids. These reactive species have been implicated in many disease conditions newlineincluding chronic inflammation, atherosclerosis, rheumatoid arthritis, some newlineneurodegenerative diseases and systemic lupus erythematosus. Systemic lupus newlineerythematosus and rheumatoid arthritis are autoimmune diseases with complex etiology newlineand pathogenesis. The abnormal level of nitrotyrosine detected in tissues affected by newlineabove diseases have been attributed to peroxynitrite mediated hypernitration of newlinetyrosine residues in proteins. Peroxynitrite is a potent oxidant as well as nitrating agent newlineand has in vivo existence. It is formed when nitric oxide reacts with superoxide radical. newlinePeroxynitrite is a powerful pro-inflammatory substance and may increase vascular newlinepermeability in inflamed tissues. newlineIn this doctoral thesis, physico-chemical and immunological studies have been newlinecarried out on histones modified by peroxynitrite with an objective of studying the newlinepossible role of oxidatively nitrated proteins in the initiation/progression of systemic newlinelupus erythematosus and rheumatoid arthritis. newlineAnalysis of UV absorption profile of peroxynitrite modified H1, H2A, H2B newlineand H3 histones revealed peak shift to higher wavelength and hyperchromicity at 276 newlinenm compared to native histones. Furthermore, in case of modified histones an newlineadditional peak was observed at 420 nm which corresponds to nitrotyrosine because a newlinestandard solution of 3-nitrotyrosine had given a similar peak under our experimental newlineconditions. The hyperchromicity observed in peroxynitrite modified histones samples newlinemight be due to nitration of tyrosine residues which ultimately enhances the molar newlineabsorptivity compared to native histones. HPLC analysis confirmed generation of newlinenitrotyrosine in peroxynitrite modified histones.

Bibliography p.142-160

Advisors/Committee Members: Alam, Khursheed.

Subjects/Keywords: Biotechnology; peroxynitrite; Antibodies; experimentally

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APA (6^th Edition):

Khan, A. (2008). Studies on experimentally produced Antibodies against peroxynitrite Modified histones; -. (Thesis). Aligarh Muslim University. Retrieved from http://shodhganga.inflibnet.ac.in/handle/10603/17961

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Khan, Asad. “Studies on experimentally produced Antibodies against peroxynitrite Modified histones; -.” 2008. Thesis, Aligarh Muslim University. Accessed January 18, 2021. http://shodhganga.inflibnet.ac.in/handle/10603/17961.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Khan, Asad. “Studies on experimentally produced Antibodies against peroxynitrite Modified histones; -.” 2008. Web. 18 Jan 2021.

Vancouver:

Khan A. Studies on experimentally produced Antibodies against peroxynitrite Modified histones; -. [Internet] [Thesis]. Aligarh Muslim University; 2008. [cited 2021 Jan 18]. Available from: http://shodhganga.inflibnet.ac.in/handle/10603/17961.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Khan A. Studies on experimentally produced Antibodies against peroxynitrite Modified histones; -. [Thesis]. Aligarh Muslim University; 2008. Available from: http://shodhganga.inflibnet.ac.in/handle/10603/17961

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Waterloo

3. Mariani, Rachelle. The role of phospholamban (PLN) in potential protection of sarc(endo)plasmic reticulum Ca2+-ATPase (SERCA) against oxidative stress.

Degree: 2015, University of Waterloo

URL: http://hdl.handle.net/10012/9144

► Sarco(endo)plasmic reticulum Ca2+ ATPase (SERCA) sequesters Ca2+ from the cytosol to: i) aid in muscular relaxation and ii) refill SR Ca2+ stores for release upon… (more)

▼ Sarco(endo)plasmic reticulum Ca2+ ATPase (SERCA) sequesters Ca2+ from the cytosol to: i) aid in muscular relaxation and ii) refill SR Ca2+ stores for release upon subsequent stimulus for contraction. SERCA function is modulated by physical interaction with phospholamban (PLN), where phosphorylation of PLN relieves its inhibitory action and increases SERCA activity. Protein-protein interactions have been shown to be protective against heat-induced SERCA2 modifications within HEK-293 cells (Fu & Tupling, 2009). Unpublished work from our lab has specifically shown SERCA2 protection in whole left ventricle (LV) when co-expressed with its regulatory modulatory protein, PLN (Gamu & Tupling, unpublished). The current thesis builds on this work by pairing vasomotor functional data from an isolated artery segment preparation with biochemical data to further explore the protective interaction between PLN and SERCA2. Peroxynitrite (ONOO-) is an oxidant known to decrease SERCA2 function through irreversible damage (Viner, Williams, & Schöneich, 1999). Thoracic aorta from wild type (WT) and phopsholamban null (PLN-/-) mice were exposed to varying concentrations of ONOO- (80 μM, 150 μM and 300 μM). Following this oxidative incubation, vasoactivity was assessed and compared. Furthermore, specific SERCA2 activity and structural adaptations through 3-NY formation were investigated. Interestingly, ONOO- incubation (150 μM) elicited genotype differences throughout the incubation period, where PLN-/- vessels had an increased basal tension response compared to WT vessels (p<0.05). Also, following 150 μM ONOO- incubation, EC50 to SNP-induced relaxation was significantly increased in PLN-/- vessels compared to WT (p<0.05). Both genotypes experienced reductions in PE-induced contractility, highlighting the ability for ONOO- incubation to decrease contractility (p<0.05) though total SERCA2 activity was significantly reduced only following the 300μM ONOO- incubation (p<0.05). No significant differences in SERCA2 activity were found between genotypes, indicating SERCA was not protected through its interaction with PLN from exposure to high ONOO-. This data shows high concentrations of ONOO-incubation is able to significantly reduce SERCA2 activity, however we only have some evidence to support that PLN may be protective. This stands as a basis for future work to be completed regarding the protective nature of PLN and SERCA2 interaction

Subjects/Keywords: SERCA; Phophsolamban; Peroxynitrite; oxidative stress; Protection

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APA (6^th Edition):

Mariani, R. (2015). The role of phospholamban (PLN) in potential protection of sarc(endo)plasmic reticulum Ca2+-ATPase (SERCA) against oxidative stress. (Thesis). University of Waterloo. Retrieved from http://hdl.handle.net/10012/9144

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Mariani, Rachelle. “The role of phospholamban (PLN) in potential protection of sarc(endo)plasmic reticulum Ca2+-ATPase (SERCA) against oxidative stress.” 2015. Thesis, University of Waterloo. Accessed January 18, 2021. http://hdl.handle.net/10012/9144.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Mariani, Rachelle. “The role of phospholamban (PLN) in potential protection of sarc(endo)plasmic reticulum Ca2+-ATPase (SERCA) against oxidative stress.” 2015. Web. 18 Jan 2021.

Vancouver:

Mariani R. The role of phospholamban (PLN) in potential protection of sarc(endo)plasmic reticulum Ca2+-ATPase (SERCA) against oxidative stress. [Internet] [Thesis]. University of Waterloo; 2015. [cited 2021 Jan 18]. Available from: http://hdl.handle.net/10012/9144.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Mariani R. The role of phospholamban (PLN) in potential protection of sarc(endo)plasmic reticulum Ca2+-ATPase (SERCA) against oxidative stress. [Thesis]. University of Waterloo; 2015. Available from: http://hdl.handle.net/10012/9144

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Georgia

4. Prakash, Roshini. Cerebrovascular remodeling and plasticity in diabetes.

Degree: 2014, University of Georgia

URL: http://hdl.handle.net/10724/28584

► We previously reported enhanced cerebrovascular remodeling and arteriogenesis in experimental type 2 diabetes. This study tested the hypotheses: 1) cerebral but not peripheral angiogenesis is… (more)

Subjects/Keywords: VEGF; peroxynitrite; cerebrovascular disease; type-2 diabetes

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APA (6^th Edition):

Prakash, R. (2014). Cerebrovascular remodeling and plasticity in diabetes. (Thesis). University of Georgia. Retrieved from http://hdl.handle.net/10724/28584

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Prakash, Roshini. “Cerebrovascular remodeling and plasticity in diabetes.” 2014. Thesis, University of Georgia. Accessed January 18, 2021. http://hdl.handle.net/10724/28584.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Prakash, Roshini. “Cerebrovascular remodeling and plasticity in diabetes.” 2014. Web. 18 Jan 2021.

Vancouver:

Prakash R. Cerebrovascular remodeling and plasticity in diabetes. [Internet] [Thesis]. University of Georgia; 2014. [cited 2021 Jan 18]. Available from: http://hdl.handle.net/10724/28584.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Prakash R. Cerebrovascular remodeling and plasticity in diabetes. [Thesis]. University of Georgia; 2014. Available from: http://hdl.handle.net/10724/28584

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Ohio University

5. Wang, Han. The Role of Nitric Oxide and Peroxynitrite in Human Embryonic Stem Cell Differentiation.

Degree: PhD, Chemistry and Biochemistry (Arts and Sciences), 2013, Ohio University

URL: http://rave.ohiolink.edu/etdc/view?acc_num=ohiou1363779566

► Human embryonic stem cells (hESCs) have the potential to grow into almost all types of cells in the human body. This special property makes hESC… (more)

▼ Human embryonic stem cells (hESCs) have the potential to grow into almost all types of cells in the human body. This special property makes hESC invaluable for the restoration of tissue function after damage from different diseases like Parkinson's disease, Alzheimer’s disease, stroke, and diabetes. The potential therapeutic applications of hESC have been hindered by a lack of knowledge of the differentiation mechanisms. It is not clear what kind of message has to be delivered to hESC to start differentiation, and what process leads to the selected cell differentiation directions. At this stage of research, it is impossible to direct the differentiation of hESC to functional specific cells for the restoration of damaged tissues. This study focused on the role of small, diffusible molecules on hESC differentiation. We hypothesized that nitric oxide (NO) and/or peroxynitrite (ONOO-) may play a role as redox mediators during early embryogenesis. After having working cell banks of mouse fibroblasts and hESCs, an eighteen-day hESC early differentiation system was established through the formation of embryoid bodies (EBs). The differentiation status of cells was evaluated through flow cytometry, proteome profiler arrays, and immunocytostaining. A nanomedical approach was used in this study. The bioavailable NO and ONOO- concentration was measured with selective electrochemical nanosensors during the differentiation process. A detailed profile of the NO and ONOO- concentrations as a function of time during the differentiation process was established. Also, several compounds which can influence the NO or ONOO- production were used to treat the cells during the differentiation process. We found that in the established differentiation system, the differentiation of hESC towards all three germ layers occurred. NO and ONOO- were both available in the microenvironment during hESC differentiation. We proved that both NO and ONOO- were crucial to the differentiation process and had a facilitating effect on the production of neuroectoderm cells. NO has a profound inhibitory effect on the production of mesoderm cells. This study is the first to demonstrate on the molecular level that both NO and ONOO- play a fundamental redox regulatory role in the early differentiation of hESCs. Advisors/Committee Members: Malinski, Tadeusz (Committee Chair).

Subjects/Keywords: Biochemistry; Nitric Oxide; Stem Cell; Peroxynitrite; Differentiation

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APA (6^th Edition):

Wang, H. (2013). The Role of Nitric Oxide and Peroxynitrite in Human Embryonic Stem Cell Differentiation. (Doctoral Dissertation). Ohio University. Retrieved from http://rave.ohiolink.edu/etdc/view?acc_num=ohiou1363779566

Chicago Manual of Style (16^th Edition):

Wang, Han. “The Role of Nitric Oxide and Peroxynitrite in Human Embryonic Stem Cell Differentiation.” 2013. Doctoral Dissertation, Ohio University. Accessed January 18, 2021. http://rave.ohiolink.edu/etdc/view?acc_num=ohiou1363779566.

MLA Handbook (7^th Edition):

Wang, Han. “The Role of Nitric Oxide and Peroxynitrite in Human Embryonic Stem Cell Differentiation.” 2013. Web. 18 Jan 2021.

Vancouver:

Wang H. The Role of Nitric Oxide and Peroxynitrite in Human Embryonic Stem Cell Differentiation. [Internet] [Doctoral dissertation]. Ohio University; 2013. [cited 2021 Jan 18]. Available from: http://rave.ohiolink.edu/etdc/view?acc_num=ohiou1363779566.

Council of Science Editors:

Wang H. The Role of Nitric Oxide and Peroxynitrite in Human Embryonic Stem Cell Differentiation. [Doctoral Dissertation]. Ohio University; 2013. Available from: http://rave.ohiolink.edu/etdc/view?acc_num=ohiou1363779566

Ohio University

6. Jiang, Lu-Lin. The Pivotal Role of Nitric Oxide and Peroxynitrite Imbalance in Epileptic Seizures.

Degree: PhD, Chemistry and Biochemistry (Arts and Sciences), 2014, Ohio University

URL: http://rave.ohiolink.edu/etdc/view?acc_num=ohiou1407723694

► Epilepsy is one of the most severe neurological disorders. However, the detailed molecular mechanism in triggering epileptic seizures is still unclear. Nitric oxide (NO) is… (more)

Subjects/Keywords: Biochemistry; Epileptic Seizure, Nitric Oxide, Peroxynitrite

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Jiang, L. (2014). The Pivotal Role of Nitric Oxide and Peroxynitrite Imbalance in Epileptic Seizures. (Doctoral Dissertation). Ohio University. Retrieved from http://rave.ohiolink.edu/etdc/view?acc_num=ohiou1407723694

Chicago Manual of Style (16^th Edition):

Jiang, Lu-Lin. “The Pivotal Role of Nitric Oxide and Peroxynitrite Imbalance in Epileptic Seizures.” 2014. Doctoral Dissertation, Ohio University. Accessed January 18, 2021. http://rave.ohiolink.edu/etdc/view?acc_num=ohiou1407723694.

MLA Handbook (7^th Edition):

Jiang, Lu-Lin. “The Pivotal Role of Nitric Oxide and Peroxynitrite Imbalance in Epileptic Seizures.” 2014. Web. 18 Jan 2021.

Vancouver:

Jiang L. The Pivotal Role of Nitric Oxide and Peroxynitrite Imbalance in Epileptic Seizures. [Internet] [Doctoral dissertation]. Ohio University; 2014. [cited 2021 Jan 18]. Available from: http://rave.ohiolink.edu/etdc/view?acc_num=ohiou1407723694.

Council of Science Editors:

Jiang L. The Pivotal Role of Nitric Oxide and Peroxynitrite Imbalance in Epileptic Seizures. [Doctoral Dissertation]. Ohio University; 2014. Available from: http://rave.ohiolink.edu/etdc/view?acc_num=ohiou1407723694

University of Arizona

7. Slosky, Lauren M. Targeting the Cystine/Glutamate Antiporter System xc⁻ in Cancer-Induced Bone Pain .

Degree: 2015, University of Arizona

URL: http://hdl.handle.net/10150/594941

► Many common cancers, including breast, prostate and lung cancers, have a propensity to metastasize to bone. Although these cancers go undetected in their native tissues,… (more)

▼ Many common cancers, including breast, prostate and lung cancers, have a propensity to metastasize to bone. Although these cancers go undetected in their native tissues, bone metastases often produce excruciating pain, the etiology of which is poorly understood. Cancer-induced bone pain (CIBP) is not well-controlled with existing medications, severely compromising patient quality of life. While CIBP is multifaceted, increased level of the excitatory neurotransmitter glutamate in the bone-tumor microenvironment may contribute to the pain state. Here, we demonstrate for the first time a relationship between reactive oxygen/nitrogen species, glutamate in the bone-tumor microenvironment and pain behaviors. The murine mammary adenocarcinoma cell line 66.1 is found to release glutamate via the cystine/glutamate antiporter system xc⁻. In a syngeneic model of breast CIBP in which 66.1 cells are inoculated into the femur intramedullary space, administration of sulfasalazine, an established system xc⁻ inhibitor and anti-inflammatory agent, reduces femur glutamate level and attenuates CIBP-related behaviors. Peroxynitrite, a reactive nitrogen species known to be generated in breast tumors, is shown to drive 66.1 system xc⁻ functional expression and tumor cell glutamate release. The elimination of peroxynitrite with the redox modulators FeTMPyP or SRI10 not only modulates tumor cell system xc⁻ functional expression in vitro and in vivo, significantly altering glutamate levels, but also assuages CIBP. In sum, we demonstrate that pharmacological inhibition of system xc⁻ transport attenuates CIBP-related behaviors. These data support a role for tumor-derived glutamate in CIBP and validate system xc⁻ an analgesic target in this pain state. Advisors/Committee Members: Vanderah, Todd W (advisor), Vanderah, Todd W. (committeemember), French, Edward D. (committeemember), Largent-Milnes, Tally M. (committeemember), Mantyh, Patrick W. (committeemember), Tome, Margaret E. (committeemember).

Subjects/Keywords: Cancer pain; Glutamate; Peroxynitrite; Peroxynitrite decomposition catalyst; Superoxide; Medical Pharmacology; Bone pain

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Slosky, L. M. (2015). Targeting the Cystine/Glutamate Antiporter System xc⁻ in Cancer-Induced Bone Pain . (Doctoral Dissertation). University of Arizona. Retrieved from http://hdl.handle.net/10150/594941

Chicago Manual of Style (16^th Edition):

Slosky, Lauren M. “Targeting the Cystine/Glutamate Antiporter System xc⁻ in Cancer-Induced Bone Pain .” 2015. Doctoral Dissertation, University of Arizona. Accessed January 18, 2021. http://hdl.handle.net/10150/594941.

MLA Handbook (7^th Edition):

Slosky, Lauren M. “Targeting the Cystine/Glutamate Antiporter System xc⁻ in Cancer-Induced Bone Pain .” 2015. Web. 18 Jan 2021.

Vancouver:

Slosky LM. Targeting the Cystine/Glutamate Antiporter System xc⁻ in Cancer-Induced Bone Pain . [Internet] [Doctoral dissertation]. University of Arizona; 2015. [cited 2021 Jan 18]. Available from: http://hdl.handle.net/10150/594941.

Council of Science Editors:

Slosky LM. Targeting the Cystine/Glutamate Antiporter System xc⁻ in Cancer-Induced Bone Pain . [Doctoral Dissertation]. University of Arizona; 2015. Available from: http://hdl.handle.net/10150/594941

University of California – Santa Cruz

8. Deboer, Tara Renee. The Design and Construction of a Biomimetic Peroxynitrite-Generating Platform: An Analytical Tool to Study the Dynamic Chemistry of Peroxynitrite.

Degree: Chemistry, 2015, University of California – Santa Cruz

URL: http://www.escholarship.org/uc/item/4tz4s8h4

► Peroxynitrite is a reactive nitrogen species that has remained at the center of controversy since the time of its discovery in the early 1990’s. The… (more)

▼ Peroxynitrite is a reactive nitrogen species that has remained at the center of controversy since the time of its discovery in the early 1990’s. The reactive nature of PN required unique chemical synthetic approaches and techniques to be developed to study the chemistry of this species. Unfortunately, many of these sources are highly alkaline, produce unwanted byproducts, and can be difficult to control. With these shortcomings in mind, a multi-well platform was designed and constructed that is capable of evolving sustained levels of peroxynitrite precursors nitric oxide and superoxide under controlled conditions. Nitric oxide and superoxide rapidly react in the wells of the platform to generate peroxynitrite in situ. This thesis describes the design process and construction of this biomimetic two-component peroxynitrite-generating system. In Chapter 2 the fabrication and characterization of the platform is discussed in detail and concludes with results obtained were intrinsic platform probe uric acid was used to effectively demonstrate the control of the system. The work highlighted in Chapter 3 establishes the application of the platform through the use of PN-target tyrosine. Investigation into PN-mediated nitration of free tyrosine and tyrosine-containing model peptides within the wells of the platform revealed extents of nitration (~40 %) significantly greater than values previously reported in the literature where alternative sources of PN were employed. Chapter 4 describes our first attempts to study an unexplored chemistry between peroxynitrite and 3-nitrotyrosine within the wells of the platform. Results from this work revealed a unique dimer product that was characterized by detailed mass spectral analysis. Based on the results of Chapters 2, 3 and 4, the designed platform has been firmly established as a powerful bioanalytical tool. Recognizing this potential, Chapter 5 describes preliminary results observed in recent studies investigating the metabolites of selected anti-inflammatory drugs upon exposure to peroxynitrite and its precursors nitric oxide and superoxide. Descriptions of the results are presented with the intentions of establishing a future direction for the peroxynitrite-generating platform.

Subjects/Keywords: Chemistry; Metal Nitrosyl; Nitric Oxide; Peroxynitrite; Platform; Superoxide

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APA (6^th Edition):

Deboer, T. R. (2015). The Design and Construction of a Biomimetic Peroxynitrite-Generating Platform: An Analytical Tool to Study the Dynamic Chemistry of Peroxynitrite. (Thesis). University of California – Santa Cruz. Retrieved from http://www.escholarship.org/uc/item/4tz4s8h4

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Deboer, Tara Renee. “The Design and Construction of a Biomimetic Peroxynitrite-Generating Platform: An Analytical Tool to Study the Dynamic Chemistry of Peroxynitrite.” 2015. Thesis, University of California – Santa Cruz. Accessed January 18, 2021. http://www.escholarship.org/uc/item/4tz4s8h4.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Deboer, Tara Renee. “The Design and Construction of a Biomimetic Peroxynitrite-Generating Platform: An Analytical Tool to Study the Dynamic Chemistry of Peroxynitrite.” 2015. Web. 18 Jan 2021.

Vancouver:

Deboer TR. The Design and Construction of a Biomimetic Peroxynitrite-Generating Platform: An Analytical Tool to Study the Dynamic Chemistry of Peroxynitrite. [Internet] [Thesis]. University of California – Santa Cruz; 2015. [cited 2021 Jan 18]. Available from: http://www.escholarship.org/uc/item/4tz4s8h4.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Deboer TR. The Design and Construction of a Biomimetic Peroxynitrite-Generating Platform: An Analytical Tool to Study the Dynamic Chemistry of Peroxynitrite. [Thesis]. University of California – Santa Cruz; 2015. Available from: http://www.escholarship.org/uc/item/4tz4s8h4

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

9. Vaz, Sandra Muntz. Oxidação e nitração de proteínas mediadas por peroxinitrito e peroxidases. Mecanismos, inibição por tempol e implicações patofisiológicas.

Degree: PhD, Bioquímica, 2008, University of São Paulo

URL: http://www.teses.usp.br/teses/disponiveis/46/46131/tde-28032008-102144/ ;

►

Os oxidantes derivados do peroxinitrito e das peroxidases, como mieloperoxidase (MPO), e os danos que ocasionam em proteínas vêm sendo muito estudados pela sua relevância… (more)

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Os oxidantes derivados do peroxinitrito e das peroxidases, como mieloperoxidase (MPO), e os danos que ocasionam em proteínas vêm sendo muito estudados pela sua relevância em processos inflamatórios. Neste trabalho, as proteínas RNase e lisozima foram empregadas como alvos de oxidação e nitração mediadas por peroxinitrito e MPO/H<SUB.2O2/NO2-. Experimentos de EPR indicaram que as oxidações envolvem a formação de radicais protéicos sendo que os principais foram caracterizados como RNase-tirosila e lisozima-tirosila exposto e não exposto ao solvente, respectivamente. Estimativas do rendimento de radicais protéicos e produtos nitrados nos pHs 5,4, 6,4 e 7,4 mostrou que o peroxinitrito e o sistema MPO/H<SUB.2O2/NO2- são oxidantes mais efetivos nos pHs 7,4 e 5,4, respectivamente. Na condição ótima para cada oxidante foram identificados produtos de oxidação/nitração de resíduos de Tyr e Trp por HPLC-UV/MS-ESI. Para localização dos resíduos modificados nas estruturas das proteínas tratadas, elas foram digeridas com tripsina e os peptídeos resultantes submetidos a análise por HPLC/MS-MALDI-ToF. Desses resultados pode-se concluir que a RNase foi nitrada preferencialmente nos fragmentos contendo o(s)resíduo(s) Tyr115 > Tyr92/97 > Tyr73/76 por peroxinitrito e em praticamente todos os resíduos de tirosina por MPOH<SUB.2O2/NO2-. No caso da lisozima, o peroxinitrito oxidou principalmente o fragmento contendo os resíduos Trp62/63 que se mostrou nitrado e oxidado a dímero e quinurenina. Já o sistema MPO/H<SUB.2O2/NO2- nitrou o fragmento contendo os resíduos Tyr23/28 e nitrou e oxidou a dímeros e quinurenina o fragmento contendo os resíduos Trp62/63. As relações entre a acessibilidade dos resíduos específicos nas estruturas terciárias e a formação de produtos de oxidação/nitração são discutidas. Também, a possível importância da oxidação de resíduos de triptofano em agregação de proteínas é enfatizada. Paralelamente, examinou-se os efeitos do nitróxido tempol sobre a nitração da RNase mediada por MPO ou HRP/H<SUB.2O2/NO2- em condições de máxima nitração. De fato, as interações de tempol com peroxidases eram pouco conhecidas apesar da eficiência do nitróxido em reduzir a injúria e os níveis de 3-nitrotirosina em proteínas de tecidos de animais submetidos a condições inflamatórias. Foram determinadas as constantes de velocidade da reação do tempol com os intermediários oxidantes da MPO e HRP e também, o consumo de reagentes e a formação de produtos. A simulação dos resultados experimentais indicou que o tempol inibe a nitração da RNase mediada por peroxidases principalmente pela sua capacidade de reagir rapidamente com o •NO2 com formação de nitrito e cátion oxamônio que, por sua vez, recicla para tempol reagindo com H2O2 para produzir O2.

The oxidants derived from peroxynitrite and peroxidase enzymes, such as myeloperoxidase (MPO), and the lesions they promote in proteins are being extensively investigated because of their relevance in inflammatory processes. Here, the proteins RNase and lysozyme were employed as targets of…

Advisors/Committee Members: Augusto, Ohara.

Subjects/Keywords: Nitração; Nitration; Oxidação; Oxidation; Peroxidases; Peroxidases; Peroxinitrito; Peroxynitrite; Tempol; Tempol

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Vaz, S. M. (2008). Oxidação e nitração de proteínas mediadas por peroxinitrito e peroxidases. Mecanismos, inibição por tempol e implicações patofisiológicas. (Doctoral Dissertation). University of São Paulo. Retrieved from http://www.teses.usp.br/teses/disponiveis/46/46131/tde-28032008-102144/ ;

Chicago Manual of Style (16^th Edition):

Vaz, Sandra Muntz. “Oxidação e nitração de proteínas mediadas por peroxinitrito e peroxidases. Mecanismos, inibição por tempol e implicações patofisiológicas.” 2008. Doctoral Dissertation, University of São Paulo. Accessed January 18, 2021. http://www.teses.usp.br/teses/disponiveis/46/46131/tde-28032008-102144/ ;.

MLA Handbook (7^th Edition):

Vaz, Sandra Muntz. “Oxidação e nitração de proteínas mediadas por peroxinitrito e peroxidases. Mecanismos, inibição por tempol e implicações patofisiológicas.” 2008. Web. 18 Jan 2021.

Vancouver:

Vaz SM. Oxidação e nitração de proteínas mediadas por peroxinitrito e peroxidases. Mecanismos, inibição por tempol e implicações patofisiológicas. [Internet] [Doctoral dissertation]. University of São Paulo; 2008. [cited 2021 Jan 18]. Available from: http://www.teses.usp.br/teses/disponiveis/46/46131/tde-28032008-102144/ ;.

Council of Science Editors:

Vaz SM. Oxidação e nitração de proteínas mediadas por peroxinitrito e peroxidases. Mecanismos, inibição por tempol e implicações patofisiológicas. [Doctoral Dissertation]. University of São Paulo; 2008. Available from: http://www.teses.usp.br/teses/disponiveis/46/46131/tde-28032008-102144/ ;

Jawaharlal Nehru University

10. Kandpal, Usha. Regulation of CD4 T cell responses; -.

Degree: immunology, 2005, Jawaharlal Nehru University

URL: http://shodhganga.inflibnet.ac.in/handle/10603/15916

None

Bibliography p. 115-133

Advisors/Committee Members: Bal, Vineeta.

Subjects/Keywords: Histocompatibility; Materials; Micromolar; Milligram; Peroxynitrite

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APA (6^th Edition):

Kandpal, U. (2005). Regulation of CD4 T cell responses; -. (Thesis). Jawaharlal Nehru University. Retrieved from http://shodhganga.inflibnet.ac.in/handle/10603/15916

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Kandpal, Usha. “Regulation of CD4 T cell responses; -.” 2005. Thesis, Jawaharlal Nehru University. Accessed January 18, 2021. http://shodhganga.inflibnet.ac.in/handle/10603/15916.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Kandpal, Usha. “Regulation of CD4 T cell responses; -.” 2005. Web. 18 Jan 2021.

Vancouver:

Kandpal U. Regulation of CD4 T cell responses; -. [Internet] [Thesis]. Jawaharlal Nehru University; 2005. [cited 2021 Jan 18]. Available from: http://shodhganga.inflibnet.ac.in/handle/10603/15916.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Kandpal U. Regulation of CD4 T cell responses; -. [Thesis]. Jawaharlal Nehru University; 2005. Available from: http://shodhganga.inflibnet.ac.in/handle/10603/15916

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

11. Khan, Md. Asad. Studies on experimentally produced antibodies against peroxynitrite modified_histones;.

Degree: Biochemistry, 2008, Aligarh Muslim University

URL: http://shodhganga.inflibnet.ac.in/handle/10603/53556

Abstract available newline newline

Bibliography p. 142-159

Advisors/Committee Members: Alam, Khursheed.

Subjects/Keywords: Produced; Antibodies; Peroxynitrite; Histones; Physiological

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Khan, M. A. (2008). Studies on experimentally produced antibodies against peroxynitrite modified_histones;. (Thesis). Aligarh Muslim University. Retrieved from http://shodhganga.inflibnet.ac.in/handle/10603/53556

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Khan, Md Asad. “Studies on experimentally produced antibodies against peroxynitrite modified_histones;.” 2008. Thesis, Aligarh Muslim University. Accessed January 18, 2021. http://shodhganga.inflibnet.ac.in/handle/10603/53556.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Khan, Md Asad. “Studies on experimentally produced antibodies against peroxynitrite modified_histones;.” 2008. Web. 18 Jan 2021.

Vancouver:

Khan MA. Studies on experimentally produced antibodies against peroxynitrite modified_histones;. [Internet] [Thesis]. Aligarh Muslim University; 2008. [cited 2021 Jan 18]. Available from: http://shodhganga.inflibnet.ac.in/handle/10603/53556.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Khan MA. Studies on experimentally produced antibodies against peroxynitrite modified_histones;. [Thesis]. Aligarh Muslim University; 2008. Available from: http://shodhganga.inflibnet.ac.in/handle/10603/53556

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

12. Khan, Md. Asad. Studies on experimentally produced antibodies against peroxynitrite modified_histones;.

Degree: Biochemistry, 2008, Aligarh Muslim University

URL: http://shodhganga.inflibnet.ac.in/handle/10603/53557

Abstract available newline newline

Bibliography p. 142-159

Advisors/Committee Members: Alam, Khursheed.

Subjects/Keywords: Produced; Antibodies; Peroxynitrite; Histones; Physiological

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Khan, M. A. (2008). Studies on experimentally produced antibodies against peroxynitrite modified_histones;. (Thesis). Aligarh Muslim University. Retrieved from http://shodhganga.inflibnet.ac.in/handle/10603/53557

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Khan, Md Asad. “Studies on experimentally produced antibodies against peroxynitrite modified_histones;.” 2008. Thesis, Aligarh Muslim University. Accessed January 18, 2021. http://shodhganga.inflibnet.ac.in/handle/10603/53557.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Khan, Md Asad. “Studies on experimentally produced antibodies against peroxynitrite modified_histones;.” 2008. Web. 18 Jan 2021.

Vancouver:

Khan MA. Studies on experimentally produced antibodies against peroxynitrite modified_histones;. [Internet] [Thesis]. Aligarh Muslim University; 2008. [cited 2021 Jan 18]. Available from: http://shodhganga.inflibnet.ac.in/handle/10603/53557.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Khan MA. Studies on experimentally produced antibodies against peroxynitrite modified_histones;. [Thesis]. Aligarh Muslim University; 2008. Available from: http://shodhganga.inflibnet.ac.in/handle/10603/53557

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

13. Khan, Humera. Immunochemical investigations on peroxynitrite _ modified immunoglobulin G;.

Degree: Biochemistry, 2010, Aligarh Muslim University

URL: http://shodhganga.inflibnet.ac.in/handle/10603/53744

Abstract available newline newline

Bibliography p. 158-183

Advisors/Committee Members: Alam, Khursheed.

Subjects/Keywords: Immunochemical; Investigations; Peroxynitrite; Immunoglobulin G

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Khan, H. (2010). Immunochemical investigations on peroxynitrite _ modified immunoglobulin G;. (Thesis). Aligarh Muslim University. Retrieved from http://shodhganga.inflibnet.ac.in/handle/10603/53744

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Khan, Humera. “Immunochemical investigations on peroxynitrite _ modified immunoglobulin G;.” 2010. Thesis, Aligarh Muslim University. Accessed January 18, 2021. http://shodhganga.inflibnet.ac.in/handle/10603/53744.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Khan, Humera. “Immunochemical investigations on peroxynitrite _ modified immunoglobulin G;.” 2010. Web. 18 Jan 2021.

Vancouver:

Khan H. Immunochemical investigations on peroxynitrite _ modified immunoglobulin G;. [Internet] [Thesis]. Aligarh Muslim University; 2010. [cited 2021 Jan 18]. Available from: http://shodhganga.inflibnet.ac.in/handle/10603/53744.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Khan H. Immunochemical investigations on peroxynitrite _ modified immunoglobulin G;. [Thesis]. Aligarh Muslim University; 2010. Available from: http://shodhganga.inflibnet.ac.in/handle/10603/53744

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Penn State University

14. Behan, Rachel Koren. SPECTROSCOPIC CHARACTERIZATION OF HIGH-VALENT INTERMEDIATES IN CYTOCHROME P450 AND OTHER HEME ENZYMES .

Degree: 2008, Penn State University

URL: https://submit-etda.libraries.psu.edu/catalog/8116

► Our group is particularly interested in the oxygen activation chemistry performed by heme peroxidases and cytochrome P450s. We set out to study compound II in… (more)

▼ Our group is particularly interested in the oxygen activation chemistry performed by heme peroxidases and cytochrome P450s. We set out to study compound II in cytochrome P450 and peroxidases because the identity of this intermediate is still the subject of debate. Spectroscopic studies on compound II in peroxidases have indicated that these intermediates are Fe(IV)oxo species. X-ray crystal structures, however, have indicated long Fe-O bonds for the ferryl intermediates in horseradish peroxidase, cytochrome c peroxidase, catalase, and myoglobin suggesting that these intermediates are protonated. To verify the X-ray crystal structure results, we performed both Mössbauer and resonance Raman spectroscopies on the same sample of ferryl myoglobin. Our experiments clearly supported the existence of only the Fe(IV)oxo species from pH 3.5 to 8.5. This result implies that the pKa of ferryl myoglobin is less than 3.5 and suggests that the long Fe-O bonds from the crystal structures are a consequence of photoreduction and does not represent the true nature of the intermediate. Although ferryl myoglobin was not protonated over a wide pH range, we have shown that compound II in cytochrome P450s is protonated at physiological pH. The ferryl intermediates in P450BM3, P450cam, and CYP158 were studied by Mössbauer spectroscopy in conjunction with DFT calculations. As was observed in chloroperoxidase compound II, a large quadrupole splitting (and an Fe(IV)-like isomer shift) was obtained for the three intermediates. The experimental Mössbauer parameters also agreed well with the theoretically determined parameters for protonated ferryl models. High-field Mössbauer measurements on P450BM3 compound II also confirmed this assignment because this intermediate had an S = 1 spin state. Based on previous experiments concerning the reaction of heme enzymes with peroxynitrite, it was evident that peroxynitrite could be used as an oxidant to generate ferryl intermediates. In our quest to study the electronic structure of P450 compound II, we utilized peroxynitrite as an oxidant to generate P450BM3 compound II. On characterization of this intermediate using UV/visible, resonance Raman, and Mössbauer spectroscopies, as well as DFT calculations, we determined that the PN intermediate in P450BM3 was not a ferryl intermediate, but rather an S = 0, ferric nitrosyl species. We studied the reaction of peroxynitrite with five other thiolate-ligated heme proteins and found similar results; however, when the reaction was performed with horseradish peroxidase and cytochrome c peroxidase (histidine-ligated heme proteins) a ferryl intermediate was found. Advisors/Committee Members: Michael Thomas Green, Committee Chair/Co-Chair, Joseph M Bollinger Jr., Committee Member, Carsten Krebs, Committee Member, Patrick Cirino, Committee Member.

Subjects/Keywords: ferryl; heme; cytochrome P450; peroxynitrite

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Behan, R. K. (2008). SPECTROSCOPIC CHARACTERIZATION OF HIGH-VALENT INTERMEDIATES IN CYTOCHROME P450 AND OTHER HEME ENZYMES . (Thesis). Penn State University. Retrieved from https://submit-etda.libraries.psu.edu/catalog/8116

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Behan, Rachel Koren. “SPECTROSCOPIC CHARACTERIZATION OF HIGH-VALENT INTERMEDIATES IN CYTOCHROME P450 AND OTHER HEME ENZYMES .” 2008. Thesis, Penn State University. Accessed January 18, 2021. https://submit-etda.libraries.psu.edu/catalog/8116.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Behan, Rachel Koren. “SPECTROSCOPIC CHARACTERIZATION OF HIGH-VALENT INTERMEDIATES IN CYTOCHROME P450 AND OTHER HEME ENZYMES .” 2008. Web. 18 Jan 2021.

Vancouver:

Behan RK. SPECTROSCOPIC CHARACTERIZATION OF HIGH-VALENT INTERMEDIATES IN CYTOCHROME P450 AND OTHER HEME ENZYMES . [Internet] [Thesis]. Penn State University; 2008. [cited 2021 Jan 18]. Available from: https://submit-etda.libraries.psu.edu/catalog/8116.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Behan RK. SPECTROSCOPIC CHARACTERIZATION OF HIGH-VALENT INTERMEDIATES IN CYTOCHROME P450 AND OTHER HEME ENZYMES . [Thesis]. Penn State University; 2008. Available from: https://submit-etda.libraries.psu.edu/catalog/8116

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Penn State University

15. Onderko, Elizabeth Lois. Selenocysteine-ligated Cytochrome P450 Intermediates.

Degree: 2015, Penn State University

URL: https://submit-etda.libraries.psu.edu/catalog/27424

► Cytochrome P450s are thiolate-ligated heme enzymes that are capable of performing challenging C-H bond activation reactions. P450s are responsible for the metabolism of many pharmaceuticals… (more)

▼ Cytochrome P450s are thiolate-ligated heme enzymes that are capable of performing challenging C-H bond activation reactions. P450s are responsible for the metabolism of many pharmaceuticals as well as the biosynthesis of steroids and other biomolecules. The electron donating ability of the thiolate ligand is believed to be responsible for their ability to oxidize unactivated C-H bonds. This proposed role of the thiolate ligand was investigated using several axial ligand variants. Cysteine-containing axial ligand variants of histidine-ligated heme enzymes were successful in reproducing some spectroscopic features of P450s, but not their reactivity. Axial ligand variants of P450s where cysteine was replaced with histidine, methionine, and serine suffered from poor heme incorporation and misfolding as well as negligible levels of monooxygenase activity. Investigations of axial ligand variants where cysteine was replaced with the more electron-donating selenocysteine indicated that these variants could still perform monooxygenase reactions. However, the intermediates directly involved, compound I and compound II, were not observed. We have produced a selenocysteine-ligated variant of the P450, CYP119, and successfully trapped a selenolate-ligated compound I species. While the UV-visible spectrum resembles the WT CYP119-I spectrum, a larger quadrupole splitting value and J/D ratio result in distinct Mössbauer and EPR spectra. Analysis of the reaction with substrate suggests that SeCYP119-I may be more reactive than the compound I of the WT enzyme. The selenolate-ligated P450 compound I species was further characterized using variable temperature Mössbauer and X-ray absorption spectroscopy (XAS). The dramatic increases in both zero field splitting and exchange coupling parameters obtained from the variable temperature Mössbauer analyses are believed to result from the intrinsic properties of the ligating selenium. However, the greater increase in J relative to D suggests that the selenocysteine ligand may allow for greater spin delocalization onto the axial ligands in compound I. The features of a selenolate-ligated P450 compound II were investigated using a selenocysteine-ligated variant of CYP158A2 (SeCYP158A2 C158S). Mössbauer and EXAFS measurements confirmed that compound II of SeCYP158A2 C158S is a ferryl hydroxide. While a pH-dependent UV-visible transition was observed, Se K-edge XANES measurements indicate that at high pH the selenocysteine ligand becomes oxidized. Peroxynitrite (PN) has been controversial in its use to generate high-valent intermediates in heme proteins. The peroxynitrite-derived P450 compound I and compound II species are unusually unreactive compared to those generated using more conventional peroxides. Furthermore, Mössbauer and UV-visible measurements have suggested that P450-PN is a nitrosyl complex. We have spectroscopically characterized P450-PN and P450-NO via Mössbauer, resonance Raman and X-ray absorption spectroscopies as well as UV-visible stopped flow spectrophotometry, confirming… Advisors/Committee Members: Michael Thomas Green, Dissertation Advisor/Co-Advisor, Michael Thomas Green, Committee Chair/Co-Chair, Carsten Krebs, Committee Member, Squire J Booker, Committee Member, Ming Tien, Committee Member.

Subjects/Keywords: cytochrome P450; selenocysteine; compound I; compound II; peroxynitrite

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Onderko, E. L. (2015). Selenocysteine-ligated Cytochrome P450 Intermediates. (Thesis). Penn State University. Retrieved from https://submit-etda.libraries.psu.edu/catalog/27424

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Onderko, Elizabeth Lois. “Selenocysteine-ligated Cytochrome P450 Intermediates.” 2015. Thesis, Penn State University. Accessed January 18, 2021. https://submit-etda.libraries.psu.edu/catalog/27424.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Onderko, Elizabeth Lois. “Selenocysteine-ligated Cytochrome P450 Intermediates.” 2015. Web. 18 Jan 2021.

Vancouver:

Onderko EL. Selenocysteine-ligated Cytochrome P450 Intermediates. [Internet] [Thesis]. Penn State University; 2015. [cited 2021 Jan 18]. Available from: https://submit-etda.libraries.psu.edu/catalog/27424.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Onderko EL. Selenocysteine-ligated Cytochrome P450 Intermediates. [Thesis]. Penn State University; 2015. Available from: https://submit-etda.libraries.psu.edu/catalog/27424

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Durham University

16. Coupe, Paul J. Mechanistic investigations of the S-nitrosothiol, peroxynitrous acid and thiol system.

Degree: PhD, 2001, Durham University

URL: http://etheses.dur.ac.uk/4211/ ; http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.369484

► S-Nitrosothiols have been found to undergo nucleophilic attack by the hydroperoxide anion to effect electrophilic nitrosation of the nucleophile. Peroxynitrite anion is formed in almost… (more)

Subjects/Keywords: 547; Thionitrite; Peroxynitrite; Nitrosation; Bromate

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Coupe, P. J. (2001). Mechanistic investigations of the S-nitrosothiol, peroxynitrous acid and thiol system. (Doctoral Dissertation). Durham University. Retrieved from http://etheses.dur.ac.uk/4211/ ; http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.369484

Chicago Manual of Style (16^th Edition):

Coupe, Paul J. “Mechanistic investigations of the S-nitrosothiol, peroxynitrous acid and thiol system.” 2001. Doctoral Dissertation, Durham University. Accessed January 18, 2021. http://etheses.dur.ac.uk/4211/ ; http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.369484.

MLA Handbook (7^th Edition):

Coupe, Paul J. “Mechanistic investigations of the S-nitrosothiol, peroxynitrous acid and thiol system.” 2001. Web. 18 Jan 2021.

Vancouver:

Coupe PJ. Mechanistic investigations of the S-nitrosothiol, peroxynitrous acid and thiol system. [Internet] [Doctoral dissertation]. Durham University; 2001. [cited 2021 Jan 18]. Available from: http://etheses.dur.ac.uk/4211/ ; http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.369484.

Council of Science Editors:

Coupe PJ. Mechanistic investigations of the S-nitrosothiol, peroxynitrous acid and thiol system. [Doctoral Dissertation]. Durham University; 2001. Available from: http://etheses.dur.ac.uk/4211/ ; http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.369484

University of Hong Kong

17. 陈幸苗. The role of peroxynitrite in neurotoxicity and neurogenesis, friend or foe?.

Degree: 2012, University of Hong Kong

URL: http://hdl.handle.net/10722/198798

► Peroxynitrite (ONOO-), produced from the reaction of superoxide anion and nitric oxide (NO), plays important roles in many physiological and pathological processes as it is… (more)

▼ Peroxynitrite (ONOO-), produced from the reaction of superoxide anion and nitric oxide (NO), plays important roles in many physiological and pathological processes as it is far more active than its precursors. However, the lack of specific and direct detection methods slow down the pace of its related research. To this end, five new peroxynitrite probes have been tested with different properties, including HKGreen-4A, 9-32, HKYellow-AM, 9-40 and MitoPN-1. Overwhelming evidence shows peroxynitrite is responsible for neurotoxicity in cerebral ischemia-reperfusion injury. Development of drugs for scavenging ONOO- becomes an important therapeutic strategy for ischemic stroke. Danshen, the dried root of salvia miltiorrhiza Bunge, is a traditional Chinese Medicine commonly used for cerebrovascular diseases. With the screening platform based on the new probe HKYellow-AM, several hydrophilic compounds from Danshen were identified to be peroxynitrite scavengers. Among them, Salvianolic acid B (SAB) possessed the highest peroxynitrite scavenging activity. Another compound, Sodium Danshensu (SDSS), the sodium salt of Danshensu, is not only a representative active compound of Danshen but also the degradation product of these hydrophilic compounds in vivo. Therefore, the potential neuroprotective effects of SAB and SDSS were studied. Both SAB and SDSS possessed strong ONOO- scavenging activity and consequently protected neuronal cell line SH-SY5Y and cortical neurons from both ONOO- and oxygen-glucose deprivation–reoxygenation induced cell death. SDSS also inhibited ONOO- formation partly by scavenging NO and superoxide. In vivo focal cerebral ischemia-reperfusion experiments revealed that both SAB and SDSS remarkably reduced protein nitration level, cell death and infarct volume in ischemia-reperfused rat brains. However, as “coin has its two sides”, whether peroxynitrite could also act as a signaling molecule has not been well known yet. The discovery of adult neurogenesis brings hope for brain repair after ischemic stroke. Recent studies indicate low concentration of peroxynitrite promotes endothelial cell growth for angiogenesis and contributes to hypoxia-induced muscle cell proliferation. To investigate the role of peroxynitrite in neurogenesis, several experiments on neural stem/progenitor cells (NSCs) were performed. The results indicated low concentrations of peroxynitrite promoted NSCs proliferation, self-renewal and neuronal differentiation. The increased peroxynitrite during hypoxia has been visualized by MitoPN-1 staining. Meanwhile, peroxynitrite decomposition catalysts (PDCs, FeTMPyP and FeTPPS) treatment reduced hypoxia-induced peroxynitrite formation, NSCs proliferation, self-renewal and neuronal differentiation. Moreover, effects of peroxynitrite on neurogenesis were partly through activating HIF-1α correlated with enhanced Wnt/β-catenin signaling pathway. In addition, the different effects of peroxynitrite on neurogenesis and neurotoxicity depended on its concentration. In summary, five new probes with…

Subjects/Keywords: Peroxynitrite; Developmental neurobiology; Neurotoxicology

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APA (6^th Edition):

陈幸苗. (2012). The role of peroxynitrite in neurotoxicity and neurogenesis, friend or foe?. (Thesis). University of Hong Kong. Retrieved from http://hdl.handle.net/10722/198798

Note: this citation may be lacking information needed for this citation format:
Author name may be incomplete
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

陈幸苗. “The role of peroxynitrite in neurotoxicity and neurogenesis, friend or foe?.” 2012. Thesis, University of Hong Kong. Accessed January 18, 2021. http://hdl.handle.net/10722/198798.

Note: this citation may be lacking information needed for this citation format:
Author name may be incomplete
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

陈幸苗. “The role of peroxynitrite in neurotoxicity and neurogenesis, friend or foe?.” 2012. Web. 18 Jan 2021.

Note: this citation may be lacking information needed for this citation format:
Author name may be incomplete

Vancouver:

陈幸苗. The role of peroxynitrite in neurotoxicity and neurogenesis, friend or foe?. [Internet] [Thesis]. University of Hong Kong; 2012. [cited 2021 Jan 18]. Available from: http://hdl.handle.net/10722/198798.

Note: this citation may be lacking information needed for this citation format:
Author name may be incomplete
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

陈幸苗. The role of peroxynitrite in neurotoxicity and neurogenesis, friend or foe?. [Thesis]. University of Hong Kong; 2012. Available from: http://hdl.handle.net/10722/198798

Note: this citation may be lacking information needed for this citation format:
Author name may be incomplete
Not specified: Masters Thesis or Doctoral Dissertation

The Ohio State University

18. Allen, Rebecca G. Macrophage Microbicidal Activity is Enhanced by Stressor-Exposure.

Degree: PhD, Integrated Biomedical Science Graduate Program, 2012, The Ohio State University

URL: http://rave.ohiolink.edu/etdc/view?acc_num=osu1324960758

► Exposure to social stressors is known to prime the innate immune system for enhanced reactivity to inflammatory stimuli, but the mechanisms by which stressor… (more)

▼ Exposure to social stressors is known to prime the innate immune system for enhanced reactivity to inflammatory stimuli, but the mechanisms by which stressor exposure can enhance immune activity are not well-defined. In mice, exposure to a social stressor called social disruption (SDR) increases circulating cytokines and primes splenic macrophages for an enhanced capacity to kill Escherichia coli, primarily through an increased production of the highly microbicidal compound peroxynitrite. Previous results demonstrate that the intestinal microbiota are in part responsible for the SDR-induced increase in circulating cytokines; reducing the microbiota through the use of a broad spectrum antibiotic cocktail prevented the SDR-induced increase in IL-6 and MCP-1. These studies tested the hypothesis that intestinal microbiota also contribute to the stressor-induced increase in the ability of splenic macrophages to kill <i>E.coli</i>. To test this hypothesis with SDR, groups of co-housed male mice were repeatedly defeated through direct interactions between the resident mice and an aggressive intruder. Following stress, <i>E. coli</i> were co-cultured with the splenic macrophages, and the number of bacteria within the macrophages was enumerated at 20 and 90 min (to determine the number of bacteria phagocytosed and then killed, respectively). We also measured changes in iNOS and pro-inflammatory cytokine expression as well as production of both superoxide anion and its reaction product with nitric oxide, peroxynitrite. When endogenous bacterial populations were eliminated through the use of germ free mice or reduced in mice treated with an antibiotic cocktail were stressed with SDR, we failed to observe the characteristic increases in pro-inflammatory cytokine and iNOS expression, superoxide anion, and peroxynitrite production. This lack of stressor-induced changes in splenic macrophage activity was associated with the failure of the stressor to enhance bacterial killing typically associated with SDR. However, when germ free mice were conventionalized to contain the normal gut microbiota and exposed to SDR, the enhanced pro-inflammatory cytokine and iNOS expression, superoxide anion, peroxynitrite, and ultimately the enhanced killing were restored. Additionally, the ability of the gut microbiota to prime splenic macrophages is associated with stressor-increased translocation of bacteria and their products from the intestinal lumen, as evidenced by an increase in circulating peptidoglycan. Importantly, the enhanced translocation of bacterial products is linked with stressor-induced mast cell degranulation and disruption of the intestinal epithelial barrier. When mast cells were inhibited from degranulating in vivo, stressor-exposure failed to enhance splenic macrophage microbicidal activity. Overall, these results indicate that the stressor-enhanced activity of CD11b+ splenic macrophages requires the degranulation of mast cells to disrupt the intestinal barrier and allow the translocation of bacteria and their… Advisors/Committee Members: Bailey, Michael (Advisor), Sheridan, John (Committee Chair).

Subjects/Keywords: Biomedical Research; Immunology; Stress; macrophage; bacterial killing; gut microbiota; peroxynitrite

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APA (6^th Edition):

Allen, R. G. (2012). Macrophage Microbicidal Activity is Enhanced by Stressor-Exposure. (Doctoral Dissertation). The Ohio State University. Retrieved from http://rave.ohiolink.edu/etdc/view?acc_num=osu1324960758

Chicago Manual of Style (16^th Edition):

Allen, Rebecca G. “Macrophage Microbicidal Activity is Enhanced by Stressor-Exposure.” 2012. Doctoral Dissertation, The Ohio State University. Accessed January 18, 2021. http://rave.ohiolink.edu/etdc/view?acc_num=osu1324960758.

MLA Handbook (7^th Edition):

Allen, Rebecca G. “Macrophage Microbicidal Activity is Enhanced by Stressor-Exposure.” 2012. Web. 18 Jan 2021.

Vancouver:

Allen RG. Macrophage Microbicidal Activity is Enhanced by Stressor-Exposure. [Internet] [Doctoral dissertation]. The Ohio State University; 2012. [cited 2021 Jan 18]. Available from: http://rave.ohiolink.edu/etdc/view?acc_num=osu1324960758.

Council of Science Editors:

Allen RG. Macrophage Microbicidal Activity is Enhanced by Stressor-Exposure. [Doctoral Dissertation]. The Ohio State University; 2012. Available from: http://rave.ohiolink.edu/etdc/view?acc_num=osu1324960758

Ohio University

19. Alhumaid, Haidar S. Nanoanalytical Studies of Bacterial Adhesion to the Membrane of Endothelial Cells.

Degree: MS, Chemistry and Biochemistry (Arts and Sciences), 2016, Ohio University

URL: http://rave.ohiolink.edu/etdc/view?acc_num=ohiou1470946411

► Adhesion of bacteria to human cells is the first step of bacterial infection. It is suspected that the endothelium is where bacterial infection takes place.… (more)

Subjects/Keywords: Chemistry; Nanoanalytical study; Adhesion of bacteria; Endothelial cells; Nitric Oxide; Peroxynitrite

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APA (6^th Edition):

Alhumaid, H. S. (2016). Nanoanalytical Studies of Bacterial Adhesion to the Membrane of Endothelial Cells. (Masters Thesis). Ohio University. Retrieved from http://rave.ohiolink.edu/etdc/view?acc_num=ohiou1470946411

Chicago Manual of Style (16^th Edition):

Alhumaid, Haidar S. “Nanoanalytical Studies of Bacterial Adhesion to the Membrane of Endothelial Cells.” 2016. Masters Thesis, Ohio University. Accessed January 18, 2021. http://rave.ohiolink.edu/etdc/view?acc_num=ohiou1470946411.

MLA Handbook (7^th Edition):

Alhumaid, Haidar S. “Nanoanalytical Studies of Bacterial Adhesion to the Membrane of Endothelial Cells.” 2016. Web. 18 Jan 2021.

Vancouver:

Alhumaid HS. Nanoanalytical Studies of Bacterial Adhesion to the Membrane of Endothelial Cells. [Internet] [Masters thesis]. Ohio University; 2016. [cited 2021 Jan 18]. Available from: http://rave.ohiolink.edu/etdc/view?acc_num=ohiou1470946411.

Council of Science Editors:

Alhumaid HS. Nanoanalytical Studies of Bacterial Adhesion to the Membrane of Endothelial Cells. [Masters Thesis]. Ohio University; 2016. Available from: http://rave.ohiolink.edu/etdc/view?acc_num=ohiou1470946411

Ohio University

20. Mahmud, Farina J. Nitric Oxide and Peroxynitrite Imbalance Triggers Cortical Hyper-Excitability and Migraine Headaches.

Degree: PhD, Chemistry and Biochemistry (Arts and Sciences), 2017, Ohio University

URL: http://rave.ohiolink.edu/etdc/view?acc_num=ohiou1481716721296335

► Migraine is a neurovascular disorder which exhibits as debilitating pain and other auxiliary symptoms. The headache is incapacitating enough to render the patient inactive and… (more)

Subjects/Keywords: Biochemistry; Neurosciences; Molecular Chemistry; Migraine; nitric oxide; peroxynitrite; cortical spreading depression

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APA (6^th Edition):

Mahmud, F. J. (2017). Nitric Oxide and Peroxynitrite Imbalance Triggers Cortical Hyper-Excitability and Migraine Headaches. (Doctoral Dissertation). Ohio University. Retrieved from http://rave.ohiolink.edu/etdc/view?acc_num=ohiou1481716721296335

Chicago Manual of Style (16^th Edition):

Mahmud, Farina J. “Nitric Oxide and Peroxynitrite Imbalance Triggers Cortical Hyper-Excitability and Migraine Headaches.” 2017. Doctoral Dissertation, Ohio University. Accessed January 18, 2021. http://rave.ohiolink.edu/etdc/view?acc_num=ohiou1481716721296335.

MLA Handbook (7^th Edition):

Mahmud, Farina J. “Nitric Oxide and Peroxynitrite Imbalance Triggers Cortical Hyper-Excitability and Migraine Headaches.” 2017. Web. 18 Jan 2021.

Vancouver:

Mahmud FJ. Nitric Oxide and Peroxynitrite Imbalance Triggers Cortical Hyper-Excitability and Migraine Headaches. [Internet] [Doctoral dissertation]. Ohio University; 2017. [cited 2021 Jan 18]. Available from: http://rave.ohiolink.edu/etdc/view?acc_num=ohiou1481716721296335.

Council of Science Editors:

Mahmud FJ. Nitric Oxide and Peroxynitrite Imbalance Triggers Cortical Hyper-Excitability and Migraine Headaches. [Doctoral Dissertation]. Ohio University; 2017. Available from: http://rave.ohiolink.edu/etdc/view?acc_num=ohiou1481716721296335

Cleveland State University

21. Kalil, Haitham Fawzy Mohamed. NANOMATERIALS-BASED SENSORS FOR PEROXYNITRITE DETECTION AND QUANTIFICATION.

Degree: PhD, College of Sciences and Health Professions, 2017, Cleveland State University

URL: http://rave.ohiolink.edu/etdc/view?acc_num=csu151336709631904

► Peroxynitrite (ONOO-, PON) plays a crucial role in several cardiovascular dysfunctions and other diseases triggered by oxidative stress. PON is a strong oxidizing agent produced… (more)

▼ Peroxynitrite (ONOO-, PON) plays a crucial role in several cardiovascular dysfunctions and other diseases triggered by oxidative stress. PON is a strong oxidizing agent produced from the diffusion-controlled reaction between nitric oxide radical and superoxide anion-radical. It is also a member of the reactive oxygen-nitrogen species family, which attacks vital components inside the body and initiates deleterious effects via direct and indirect interactions. PON reacts directly with lipids, DNA, and proteins, whereas indirectly, it acts as an initiator of radical-chain reactions.In this work, we have explored various interfaces of manganese-oxide-decorated graphene/hemin and selenium-containing compound for PON detection and quantification. The combination of manganese oxide nanoparticles with the graphene/hemin matrix has allowed for more hemin molecules to be adsorbed on the final composite matrix. As a result, the adsorbed hemin has enhanced the catalytic activity of the final composite and improved the sensitivity towards PON detection. In the same context, a selenium-containing compound (aniline-selenide) has been synthesized and grafted on the electrode surface using the chemistry of diazonium salt. The aniline-selenide-modified electrode showed an increase of approximately 40 times the catalytic current as the aniline-modified electrode.Throughout this project, the preparation methods of the electroactive nanomaterials were described in detail. Moreover, the characterizations of the prepared-materials have been investigated by various physicochemical methods using scanning electron microscopy (SEM), energy dispersive X-ray spectroscopy (EDS), ultraviolet/visible measurements (UV/Vis), and X-ray photoelectron spectroscopy (XPS).This study showed the importance of using selenium and manganese interfaces as sensitive platforms for PON detection. It also provides the initial stage to extend the use of these interfaces to ultramicroelectrodes sensors for use at the level of single cells. Advisors/Committee Members: Bayachou, Mekki (Committee Chair).

Subjects/Keywords: Chemistry; Peroxynitrite; Cyclic Voltammetry; Manganese functionalized graphene-hemin; Aniline selenide

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APA (6^th Edition):

Kalil, H. F. M. (2017). NANOMATERIALS-BASED SENSORS FOR PEROXYNITRITE DETECTION AND QUANTIFICATION. (Doctoral Dissertation). Cleveland State University. Retrieved from http://rave.ohiolink.edu/etdc/view?acc_num=csu151336709631904

Chicago Manual of Style (16^th Edition):

Kalil, Haitham Fawzy Mohamed. “NANOMATERIALS-BASED SENSORS FOR PEROXYNITRITE DETECTION AND QUANTIFICATION.” 2017. Doctoral Dissertation, Cleveland State University. Accessed January 18, 2021. http://rave.ohiolink.edu/etdc/view?acc_num=csu151336709631904.

MLA Handbook (7^th Edition):

Kalil, Haitham Fawzy Mohamed. “NANOMATERIALS-BASED SENSORS FOR PEROXYNITRITE DETECTION AND QUANTIFICATION.” 2017. Web. 18 Jan 2021.

Vancouver:

Kalil HFM. NANOMATERIALS-BASED SENSORS FOR PEROXYNITRITE DETECTION AND QUANTIFICATION. [Internet] [Doctoral dissertation]. Cleveland State University; 2017. [cited 2021 Jan 18]. Available from: http://rave.ohiolink.edu/etdc/view?acc_num=csu151336709631904.

Council of Science Editors:

Kalil HFM. NANOMATERIALS-BASED SENSORS FOR PEROXYNITRITE DETECTION AND QUANTIFICATION. [Doctoral Dissertation]. Cleveland State University; 2017. Available from: http://rave.ohiolink.edu/etdc/view?acc_num=csu151336709631904

Kent State University

22. Mukherjee, Riya. Studies on the Coordination Chemistry of Vanadium, Barium and Cobalamins.

Degree: PhD, College of Arts and Sciences / Department of Chemistry, 2011, Kent State University

URL: http://rave.ohiolink.edu/etdc/view?acc_num=kent1300909587

► Part 1 of this dissertation concerns studies on polynuclear V(III)/carboxylato complexes (Chapter 2) and a Ba(II)/thiodiacetato complex (Chapter 3) formed in aqueous solution. The… (more)

▼ Part 1 of this dissertation concerns studies on polynuclear V(III)/carboxylato complexes (Chapter 2) and a Ba(II)/thiodiacetato complex (Chapter 3) formed in aqueous solution. The structures of[V3(µ3-O)(µ-OOCCH2Br)6(OH2)3]CF3SO3•H2O and [V3(µ3-O)(µ-OOCCH2CH3)6(OH2)3]Cl•2H2O have been determined by X-ray diffraction in chapter 2. Electrospray mass spectrometry, 1H NMR and UV-vis spectroscopy and diffusion coefficient measurements suggest that trinuclear and tetranuclear V(III)/carboxylato complexes are present in solution. Chapter 3 concerns the synthesis of polymeric {Ba[S(CH2COO)2(H2O)3]•2H2O}n, characterized by X-ray diffraction, elemental analysis, FT-IR spectroscopy, thermogravimetric analysis and 1H and 13C NMR spectroscopy. Ba2+ is ten-coordinate, ligated to four tda (O and S) and three aqua ligands. Bridging of Ba2+ centers via the tda ligand creates an extended 2-D polymeric layered structure. Part 2 of this dissertation is concerned with cobalamin (= vitamin B12) chemistry. The development of vanadate (V(V)) therapeutics as oral insulin enhancing agents substitutes for treating diabetes is an active research field. By coordinating the therapeutic to the β axial site of cobalamin, the absorption and cellular uptake is significantly enhanced. In Chapter 4, the extensive solution characterization of two vanadate/B12 conjugates incorporating a (3-hydroxy-2-methyl-1H-pyridin-4-one)propyl linker is reported. The conjugates are tested in the streptozotocin rat model for Type 1 diabetes. The orally administered therapeutic captopril is widely used for treating hypertension and cardiovascular disease, with undesirable side effects. Chapter 5 reports the synthesis of captoprilcobalamin (CapSCbl). Characterization by 1H NMR spectroscopy and X-ray diffraction shows that CapSCbl exists as two geometric isomers. Kinetic studies on the formation of CapSCbl and its decomposition in acidic solution are also reported. The last two chapters report rate constants and proposed mechanisms for the reactions of peroxynitrite/peroxynitrous acid (ONOO-/ONOOH) with the reduced forms of vitamin B12, cob(II)alamin (Chapter 6) and cob(I)alamin (Chapter 7). ONOO-/ONOOH is implicated in multiple inflammatory and neurodegenerative diseases. ONOOH reacts directly with Cb(II) to give cob(III)alamin and •NO2, followed by a rapid reaction between •NO2 and Cbl(II) to primarily form nitrocobalamin. A mechanism involving rate-determining 1e- oxidation of Cbl(I) by ONOO(H) to yield Cbl(I) and •NO2 followed by multiple fast steps leading ultimately to oxidation of 5Cbl(I) to 5Cbl(II) and N2 formation is proposed. Advisors/Committee Members: Brasch, Nicola E. (Advisor).

Subjects/Keywords: Inorganic Chemistry; cobalamins; peroxynitrite; bioconjugates; vanadium; barium; captopril

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APA (6^th Edition):

Mukherjee, R. (2011). Studies on the Coordination Chemistry of Vanadium, Barium and Cobalamins. (Doctoral Dissertation). Kent State University. Retrieved from http://rave.ohiolink.edu/etdc/view?acc_num=kent1300909587

Chicago Manual of Style (16^th Edition):

Mukherjee, Riya. “Studies on the Coordination Chemistry of Vanadium, Barium and Cobalamins.” 2011. Doctoral Dissertation, Kent State University. Accessed January 18, 2021. http://rave.ohiolink.edu/etdc/view?acc_num=kent1300909587.

MLA Handbook (7^th Edition):

Mukherjee, Riya. “Studies on the Coordination Chemistry of Vanadium, Barium and Cobalamins.” 2011. Web. 18 Jan 2021.

Vancouver:

Mukherjee R. Studies on the Coordination Chemistry of Vanadium, Barium and Cobalamins. [Internet] [Doctoral dissertation]. Kent State University; 2011. [cited 2021 Jan 18]. Available from: http://rave.ohiolink.edu/etdc/view?acc_num=kent1300909587.

Council of Science Editors:

Mukherjee R. Studies on the Coordination Chemistry of Vanadium, Barium and Cobalamins. [Doctoral Dissertation]. Kent State University; 2011. Available from: http://rave.ohiolink.edu/etdc/view?acc_num=kent1300909587

23. Tokikawa, Rita. Acetilação radicalar de amino ácidos, peptídeos e nucleobases pelos sistemas biacetilo/peroxinitrito e metilglioxal/peroxinitrito.

Degree: PhD, Bioquímica, 2012, University of São Paulo

URL: http://www.teses.usp.br/teses/disponiveis/46/46131/tde-04092012-144221/ ;

►

Biacetilo (2,3-butanediona) é um contaminante de comida e cigarro, também implicado na hepatoxicidade do álcool e em doenças pulmonares. O metilglioxal (MG), um α-oxoaldeído reativo… (more)

▼

Biacetilo (2,3-butanediona) é um contaminante de comida e cigarro, também implicado na hepatoxicidade do álcool e em doenças pulmonares. O metilglioxal (MG), um α-oxoaldeído reativo frequentemente associado ao diabetes e envelhecimento, é produto da fragmentação oxidativa de trioses fosfato, acetona e aminoacetona. Por sua vez, peroxinitrito - um potente oxidante, agente nitrante e nucleófilo formado in vivo pela reação controlada por difusão do ânion radical superóxido com o radical óxido nítrico (k ~1010 M-1s-1) é capaz de se adicionar a CO2 e compostos carbonílicos, gerando produtos potencialmente tóxicos ou sinalizadores celulares. Aminoácidos, peptídeos e nucleobases podem ser acetilados nos grupos amina e na porção desoxiribose. Relativamente ao tratamento com peroxinitrito isolado, níveis superiores de 3-nitrotirosina foram detectados quando tirosina foi tratada com peroxinitrito/biacetilo ou metilglioxal. Ambos os grupos amina de lisina (Lys) ou um deles de derivados de lisina bloqueados e um deles (Ac-Lys-OMe, Z-Lys-OMe) foram acetilados pelo sistema biacetilo ou metilglioxal/peroxinitrito. Em tetrapeptídeos sintéticos contendo lisina como aminoácido amino-terminal (H-KALA-OH, Ac-KALA-OH and H-K(Boc)ALA-OH), a lisina foi acetilada pelo sistemas dicarbonilico/peroxinitrito no grupo α-amina (em maior extensão) e/ou no ε-amina (em menor extensão). No conjunto, estes resultados podem ser interpretados à luz do mecanismo proposto para a reação de compostos α-dicarbonílicos com peroxinitrito, o qual envolve sequencialmente: (i) adição nucleofílica de peroxinitrito à carbonila; (ii) homólise do aduto peroxinitroso formado, liberando •NO2 e um radical oxila do reagente carbonílico; (iii) β-clivagem do radical oxila a um ácido carboxílico (ácido acético no caso de biacetilo e ácido fórmico, a partir de metilglioxal) e radical acetila; (iv) captação do radical acetila pelo oxigênio molecular dissolvido dando acetato, ou por aminoácido ou nucleobase, se presentes, gerando o produto acetilado. Tais resultados são interessantes ao levantar a hipótese de acetilação radicalar como mecanismo de modificação pós-traducional de proteínas, até então considerado um processo realizado apenas por acetilases.

Diacetyl (2,3-butanedione) is a food and cigarette contaminant recently implicated in alcohol hepatotoxicity and lung disease. In turn, methylglyoxal (MG) is an α-oxoaldehyde frequently associated with diabetes and aging that is putatively formed by the oxidative fragmentation of trioses phosphate, acetone and aminoacetone. Peroxynitrite - a potent oxidant, nitrating agent and nucleophile formed in vivo by the diffusion-controlled reaction of superoxide radical with nitric oxide (k ~1010 M-1s-1) - is able to form adducts with carbon dioxide and carbonyl compounds. When initially present in the reaction mixtures before addition of ONOO-, amino acids, peptides and nucleobases undergo acetylation at the amino group and purine moieties in the presence of biacetyl or methylglyoxal. Higher…

Advisors/Committee Members: Bechara, Etelvino Jose Henriques, Machini, Maria Teresa.

Subjects/Keywords: Acetilação radicalar; Biacetilo; Biacetyl; Biochemistry; Bioquímica; Lisina; Lysine; Methylglyoxal; Metilglioxal; Peroxinitrito; Peroxynitrite; Radical Acetylation

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APA (6^th Edition):

Tokikawa, R. (2012). Acetilação radicalar de amino ácidos, peptídeos e nucleobases pelos sistemas biacetilo/peroxinitrito e metilglioxal/peroxinitrito. (Doctoral Dissertation). University of São Paulo. Retrieved from http://www.teses.usp.br/teses/disponiveis/46/46131/tde-04092012-144221/ ;

Chicago Manual of Style (16^th Edition):

Tokikawa, Rita. “Acetilação radicalar de amino ácidos, peptídeos e nucleobases pelos sistemas biacetilo/peroxinitrito e metilglioxal/peroxinitrito.” 2012. Doctoral Dissertation, University of São Paulo. Accessed January 18, 2021. http://www.teses.usp.br/teses/disponiveis/46/46131/tde-04092012-144221/ ;.

MLA Handbook (7^th Edition):

Tokikawa, Rita. “Acetilação radicalar de amino ácidos, peptídeos e nucleobases pelos sistemas biacetilo/peroxinitrito e metilglioxal/peroxinitrito.” 2012. Web. 18 Jan 2021.

Vancouver:

Tokikawa R. Acetilação radicalar de amino ácidos, peptídeos e nucleobases pelos sistemas biacetilo/peroxinitrito e metilglioxal/peroxinitrito. [Internet] [Doctoral dissertation]. University of São Paulo; 2012. [cited 2021 Jan 18]. Available from: http://www.teses.usp.br/teses/disponiveis/46/46131/tde-04092012-144221/ ;.

Council of Science Editors:

Tokikawa R. Acetilação radicalar de amino ácidos, peptídeos e nucleobases pelos sistemas biacetilo/peroxinitrito e metilglioxal/peroxinitrito. [Doctoral Dissertation]. University of São Paulo; 2012. Available from: http://www.teses.usp.br/teses/disponiveis/46/46131/tde-04092012-144221/ ;

University of Georgia

24. Hartman, Nathan. The effect of nitrosative and oxidative stress on Helicobacter pylori.

Degree: 2016, University of Georgia

URL: http://hdl.handle.net/10724/35728

► The gastric pathogen Helicobacter pylori is able to withstand an onslaught of oxidative and nitrosative stress from the host immune system. One such stress arises… (more)

Subjects/Keywords: Helicobacter pylori; Peroxynitrite; Tyrosine nitration; Oxidative stress; Protein L-isoaspartate methyltransferase; Isoaspartate

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APA (6^th Edition):

Hartman, N. (2016). The effect of nitrosative and oxidative stress on Helicobacter pylori. (Thesis). University of Georgia. Retrieved from http://hdl.handle.net/10724/35728

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Hartman, Nathan. “The effect of nitrosative and oxidative stress on Helicobacter pylori.” 2016. Thesis, University of Georgia. Accessed January 18, 2021. http://hdl.handle.net/10724/35728.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Hartman, Nathan. “The effect of nitrosative and oxidative stress on Helicobacter pylori.” 2016. Web. 18 Jan 2021.

Vancouver:

Hartman N. The effect of nitrosative and oxidative stress on Helicobacter pylori. [Internet] [Thesis]. University of Georgia; 2016. [cited 2021 Jan 18]. Available from: http://hdl.handle.net/10724/35728.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Hartman N. The effect of nitrosative and oxidative stress on Helicobacter pylori. [Thesis]. University of Georgia; 2016. Available from: http://hdl.handle.net/10724/35728

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Saskatchewan

25. Polewicz, Dorota Katarzyna. Oxidative stress-induced, peroxynitrite-dependent, modifications of myosin light chain 1 lead to its increased degradation by matrix metalloproteinase-2.

Degree: 2010, University of Saskatchewan

URL: http://hdl.handle.net/10388/etd-06232010-154218

► Damage to cardiac contractile proteins such as myosin light chain 1 (MLC1), during oxidative stress is mediated by reactive oxygen species such as peroxynitrite (ONOO-),… (more)

Subjects/Keywords: MLC1; oxidative stress; MMP-2; peroxynitrite

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APA (6^th Edition):

Polewicz, D. K. (2010). Oxidative stress-induced, peroxynitrite-dependent, modifications of myosin light chain 1 lead to its increased degradation by matrix metalloproteinase-2. (Thesis). University of Saskatchewan. Retrieved from http://hdl.handle.net/10388/etd-06232010-154218

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Polewicz, Dorota Katarzyna. “Oxidative stress-induced, peroxynitrite-dependent, modifications of myosin light chain 1 lead to its increased degradation by matrix metalloproteinase-2.” 2010. Thesis, University of Saskatchewan. Accessed January 18, 2021. http://hdl.handle.net/10388/etd-06232010-154218.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Polewicz, Dorota Katarzyna. “Oxidative stress-induced, peroxynitrite-dependent, modifications of myosin light chain 1 lead to its increased degradation by matrix metalloproteinase-2.” 2010. Web. 18 Jan 2021.

Vancouver:

Polewicz DK. Oxidative stress-induced, peroxynitrite-dependent, modifications of myosin light chain 1 lead to its increased degradation by matrix metalloproteinase-2. [Internet] [Thesis]. University of Saskatchewan; 2010. [cited 2021 Jan 18]. Available from: http://hdl.handle.net/10388/etd-06232010-154218.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Polewicz DK. Oxidative stress-induced, peroxynitrite-dependent, modifications of myosin light chain 1 lead to its increased degradation by matrix metalloproteinase-2. [Thesis]. University of Saskatchewan; 2010. Available from: http://hdl.handle.net/10388/etd-06232010-154218

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Ohio University

26. Tang, Yuanyuan. Nitric Oxide/Peroxynitrite Imbalance Induces Adhesion of Cancer Cells to Lymphatic Endothelium - Clinical Implications for Cancer Metastasis.

Degree: PhD, Chemistry and Biochemistry (Arts and Sciences), 2015, Ohio University

URL: http://rave.ohiolink.edu/etdc/view?acc_num=ohiou1439563414

► Adhesion of cancer cells to endothelial cells is a key step in metastasis. However, the molecular mechanisms mediating cancer cell/endothelial cell interaction and adhesion process… (more)

Subjects/Keywords: Chemistry; Biochemistry; Analytical Chemistry; nitric oxide; peroxynitrite; cell adhesion; lymphatic endothelial cells; cancer cells

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APA (6^th Edition):

Tang, Y. (2015). Nitric Oxide/Peroxynitrite Imbalance Induces Adhesion of Cancer Cells to Lymphatic Endothelium - Clinical Implications for Cancer Metastasis. (Doctoral Dissertation). Ohio University. Retrieved from http://rave.ohiolink.edu/etdc/view?acc_num=ohiou1439563414

Chicago Manual of Style (16^th Edition):

Tang, Yuanyuan. “Nitric Oxide/Peroxynitrite Imbalance Induces Adhesion of Cancer Cells to Lymphatic Endothelium - Clinical Implications for Cancer Metastasis.” 2015. Doctoral Dissertation, Ohio University. Accessed January 18, 2021. http://rave.ohiolink.edu/etdc/view?acc_num=ohiou1439563414.

MLA Handbook (7^th Edition):

Tang, Yuanyuan. “Nitric Oxide/Peroxynitrite Imbalance Induces Adhesion of Cancer Cells to Lymphatic Endothelium - Clinical Implications for Cancer Metastasis.” 2015. Web. 18 Jan 2021.

Vancouver:

Tang Y. Nitric Oxide/Peroxynitrite Imbalance Induces Adhesion of Cancer Cells to Lymphatic Endothelium - Clinical Implications for Cancer Metastasis. [Internet] [Doctoral dissertation]. Ohio University; 2015. [cited 2021 Jan 18]. Available from: http://rave.ohiolink.edu/etdc/view?acc_num=ohiou1439563414.

Council of Science Editors:

Tang Y. Nitric Oxide/Peroxynitrite Imbalance Induces Adhesion of Cancer Cells to Lymphatic Endothelium - Clinical Implications for Cancer Metastasis. [Doctoral Dissertation]. Ohio University; 2015. Available from: http://rave.ohiolink.edu/etdc/view?acc_num=ohiou1439563414

The Ohio State University

27. Kohr, Mark Jeffrey, Jr. Modulation of Cardiac Contraction by Reactive Nitrogen Species.

Degree: PhD, Integrated Biomedical Sciences, 2009, The Ohio State University

URL: http://rave.ohiolink.edu/etdc/view?acc_num=osu1237753236

► Reactive nitrogen species, including peroxynitrite and nitroxyl, have been demonstrated to affect myocardial contractility. Many of these reactive nitrogen species are produced endogenously within… (more)

▼ Reactive nitrogen species, including peroxynitrite and nitroxyl, have been demonstrated to affect myocardial contractility. Many of these reactive nitrogen species are produced endogenously within cardiac myocytes and serve as critical regulators of myocyte function. Alterations in the production of these reactive species may be responsible for part of the dysfunction that is observed during many pathophysiological conditions of the myocardium. Although studies have partially documented the contractile effects of many different reactive nitrogen species, few have examined the specific mechanism of action. Therefore, in order to address deficiencies in regards to the physiological and pathophysiological regulation of myocardial function by reactive nitrogen species, we set out to characterize the contractile effects and specific mechanism of action for two distinct reactive nitrogen species, peroxynitrite and nitroxyl. We first characterized the effects of both high and low concentrations of peroxynitrite on cardiac myocyte function during three different contractile states (basal, submaximal and maximal beta-adrenergic stimulation). High peroxynitrite produced a negative effect on myocyte function that became more pronounced as the contractile state in the cardiac myocyte was increased. Conversely, low peroxynitrite produced a positive effect on myocyte function that was greatest during basal contraction and diminished as the contractile state in the cardiac myocyte was increased. Interestingly, the effects of both high and low peroxynitrite were completely absent during all contractile states in phospholamban knockout myocytes. These results indicate that high and low peroxynitrite both exert effects on cardiac myocyte contraction by targeting the critical excitation-contraction coupling phosphoprotein, phospholamban. In order to elucidate the specific signaling pathway of high peroxynitrite, we examined phospholamban phosphorylation at the cAMP-dependent kinase site (serine 16) and observed that high peroxynitrite decreased beta-adrenergic-stimulated phospholamban phosphorylation. However, protein phosphatase inhibition (okadaic acid) inhibited the negative effects of high peroxynitrite on myocyte contraction and phospholamban phosphorylation. High peroxynitrite also increased total protein phosphatase activity and promoted the interaction between phospholamban and protein phosphatase 2a. Therefore, high levels of peroxynitrite decrease beta-adrenergic-stimulated myocyte contraction by inducing the dephosphorylation of phospholamban via protein phosphatase 2a activation. We next examined the specific signaling pathway underlying the effects of low peroxynitrite. Since we determined that the effects of high peroxynitrite occurred through a protein phosphatase-dependent mechanism, we repeated our functional measurements in the presence of protein phosphatase inhibition. However, protein phosphatase inhibition did not alter the positive effect of low peroxynitrite on myocyte function. Low… Advisors/Committee Members: Ziolo, Mark (Advisor).

Subjects/Keywords: Biomedical Research; nitric oxide; peroxynitrite; nitroxyl

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Kohr, Mark Jeffrey, J. (2009). Modulation of Cardiac Contraction by Reactive Nitrogen Species. (Doctoral Dissertation). The Ohio State University. Retrieved from http://rave.ohiolink.edu/etdc/view?acc_num=osu1237753236

Chicago Manual of Style (16^th Edition):

Kohr, Mark Jeffrey, Jr. “Modulation of Cardiac Contraction by Reactive Nitrogen Species.” 2009. Doctoral Dissertation, The Ohio State University. Accessed January 18, 2021. http://rave.ohiolink.edu/etdc/view?acc_num=osu1237753236.

MLA Handbook (7^th Edition):

Kohr, Mark Jeffrey, Jr. “Modulation of Cardiac Contraction by Reactive Nitrogen Species.” 2009. Web. 18 Jan 2021.

Vancouver:

Kohr, Mark Jeffrey J. Modulation of Cardiac Contraction by Reactive Nitrogen Species. [Internet] [Doctoral dissertation]. The Ohio State University; 2009. [cited 2021 Jan 18]. Available from: http://rave.ohiolink.edu/etdc/view?acc_num=osu1237753236.

Council of Science Editors:

Kohr, Mark Jeffrey J. Modulation of Cardiac Contraction by Reactive Nitrogen Species. [Doctoral Dissertation]. The Ohio State University; 2009. Available from: http://rave.ohiolink.edu/etdc/view?acc_num=osu1237753236

University of California – Riverside

28. Chen, Zhijie. Illuminating Biology With Engineered Fluorescent Proteins.

Degree: Chemistry, 2016, University of California – Riverside

URL: http://www.escholarship.org/uc/item/3zp2n2zt

► Fluorescent proteins (FPs) emerged in the mid-90s as a powerful tool for life science research. Among its numerous applications, FPs is best known as reporter… (more)

▼ Fluorescent proteins (FPs) emerged in the mid-90s as a powerful tool for life science research. Among its numerous applications, FPs is best known as reporter genes to study the localization and dynamics of proteins. In this dissertation, we explore new uses of FPs as genetically encoded fluorescent probes to image cellular chemical species. In chapter 2, we incorporated of p-azidophenylalanine (pAzF) into circularly permuted GFP (cpGFP), which gave rise to the first genetically encoded fluorescent probe for H2S. Further engineering of the cpGFP scaffold, followed by directed evolution, has resulted in an improved fluorescent probe for H2S–hsGFP. In chapter 3, we incorporated p-boronophenylalanine (pBoF) into different cpGFP templates, which serendipitously resulted in pnGFP, the first genetically encoded fluorescent probe for the selective detection of peroxynitrite (ONOO–). In chapter 4, we examined the molecular mechanism underlying the unusual chemoselectivity of pnGFP towards ONOO– over H2O2 by using site-directed mutagenesis, X-ray crystallography, 11B NMR, and computational simulation studies. Surprisingly, the data collectively support an sp3-hybridized boron in pnGFP through a water bridge mediated N-B interaction between His 9 and the boronic acid moiety on the chromophore. In chapter 5, we described the engineering and biological application of “G-CaMP”-type indicators for zinc ion (Zn2+). Insertion of a naturally evolved zinc-binding domain into the β-barrel of single green or red FP, followed by directed protein evolution and functional screening, we identified single-FP based green and red Zn2+ indicators, ZnGreen and ZnRed. These optically compatible indicators enabled dual color imaging of Zn2+ at single cell resolution. We further demonstrated the use of ZnGreen1 in imaging of glucose-stimulated Zn2+ secretion in pancreatic β cells. Finally, in chapter 6, we laid out challenges in the field and provide future outlooks on possible new directions.

Subjects/Keywords: Chemistry; Biochemistry; Biology; Fluorescent Proteins; Genetically Encoded Fluorescent Probes; Hydrogen Sulfide; Peroxynitrite; Unnatural Amino Acids; Zinc Ion

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Chen, Z. (2016). Illuminating Biology With Engineered Fluorescent Proteins. (Thesis). University of California – Riverside. Retrieved from http://www.escholarship.org/uc/item/3zp2n2zt

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Chen, Zhijie. “Illuminating Biology With Engineered Fluorescent Proteins.” 2016. Thesis, University of California – Riverside. Accessed January 18, 2021. http://www.escholarship.org/uc/item/3zp2n2zt.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Chen, Zhijie. “Illuminating Biology With Engineered Fluorescent Proteins.” 2016. Web. 18 Jan 2021.

Vancouver:

Chen Z. Illuminating Biology With Engineered Fluorescent Proteins. [Internet] [Thesis]. University of California – Riverside; 2016. [cited 2021 Jan 18]. Available from: http://www.escholarship.org/uc/item/3zp2n2zt.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Chen Z. Illuminating Biology With Engineered Fluorescent Proteins. [Thesis]. University of California – Riverside; 2016. Available from: http://www.escholarship.org/uc/item/3zp2n2zt

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Rochester

29. Barth, Kenneth Raymond. Nitric Oxide Metabolism and Resistance to Peroxynitrite by Neisseria Species.

Degree: PhD, 2009, University of Rochester

URL: http://hdl.handle.net/1802/8461

► Neisseria gonorrhoeae is the causative agent of gonorrhea, a sexually transmitted disease that primarily infects the urogenital tract. Prolonged infection often leads to complications, such… (more)

▼ Neisseria gonorrhoeae is the causative agent of gonorrhea, a sexually transmitted disease that primarily infects the urogenital tract. Prolonged infection often leads to complications, such as disseminated gonococcal infection and pelvic inflammatory disease. Women specifically have high rates of asymptomatic disease that we believe are partially an effect of bacterial metabolism of nitric oxide, considering nitric oxide levels directly correlate with immune system state of activation. The central role of nitric oxide within the immune system involves not only its signaling properties, but also its toxic properties. My studies have focused on examining the ability of Neisseria species to both produce and degrade nitric oxide, while establishing a nitric oxide steady-state in the presence of nitrite or an exogenous source of nitric oxide, as well as its resistance to the toxic nitrogen species, nitric oxide and peroxynitrite. Both pathogenic and commensal Neisseria species were found to have similar enzymatic activities for the production and degradation of nitric oxide, in addition to being equally efficient at modulating nitric oxide levels from pro-inflammatory concentrations to non-inflammatory concentrations during denitrification. Auxotrophic strains requiring arginine, hypoxanthine, and uracil were found to establish unusually low nitric oxide steady-states in the presence of nitrite. These same strains have been correlated with causing asymptomatic infections in men, meaning that even in the presence of high levels of nitrite, nitric oxide levels would remain in the range where it is involved in establishing non-inflammatory conditions. Respiratory control was shown to have a direct influence on denitrification, causing changes to the nitric oxide steady-state level and the activities of nitrite reductase and nitric oxide reductase. The toxic properties of nitric oxide include additional direct and indirect effects as a result of generation of other reactive nitrogen species being generated, such as peroxynitrite. This strong oxidant represents a valuable means for the immune system to control and eradicate bacterial pathogens. N. gonorrhoeae was shown to have a high level of resistance to peroxynitrite, while N. meningitidis and E. coli showed high sensitivity to the reactive species. Mutational analysis of gonococcal orthologs to genes identified to be involved in nitrosative defense in other bacteria had no effect on peroxynitrite resistance in N. gonorrhoeae. This suggests that the gonococcus has redundant and/or unique mechanisms to survive the bactericidal effects of peroxynitrite that would allow for increased persistence of the pathogen.

Subjects/Keywords: Neisseria Gonorrhoeae; Nitric Oxide; Denitrification; RNS; Peroxynitrite

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Barth, K. R. (2009). Nitric Oxide Metabolism and Resistance to Peroxynitrite by Neisseria Species. (Doctoral Dissertation). University of Rochester. Retrieved from http://hdl.handle.net/1802/8461

Chicago Manual of Style (16^th Edition):

Barth, Kenneth Raymond. “Nitric Oxide Metabolism and Resistance to Peroxynitrite by Neisseria Species.” 2009. Doctoral Dissertation, University of Rochester. Accessed January 18, 2021. http://hdl.handle.net/1802/8461.

MLA Handbook (7^th Edition):

Barth, Kenneth Raymond. “Nitric Oxide Metabolism and Resistance to Peroxynitrite by Neisseria Species.” 2009. Web. 18 Jan 2021.

Vancouver:

Barth KR. Nitric Oxide Metabolism and Resistance to Peroxynitrite by Neisseria Species. [Internet] [Doctoral dissertation]. University of Rochester; 2009. [cited 2021 Jan 18]. Available from: http://hdl.handle.net/1802/8461.

Council of Science Editors:

Barth KR. Nitric Oxide Metabolism and Resistance to Peroxynitrite by Neisseria Species. [Doctoral Dissertation]. University of Rochester; 2009. Available from: http://hdl.handle.net/1802/8461

30. Massari Filho, Júlio. Mecanismos da reação de metabólitos α-dicarbonílicos com peroxinitrito: geração de radicais livres e oxigênio singlete. Possíveis implicações biológicas.

Degree: PhD, Química, 2014, University of São Paulo

URL: http://www.teses.usp.br/teses/disponiveis/46/46136/tde-02102014-081602/ ;

►

Peroxinitrito é um potente agente oxidante, nitrante e nucleofilico formado in vivo pela reação difusional do radical ânion superóxido com óxido nítrico, cuja produção exacerbada… (more)

▼

Peroxinitrito é um potente agente oxidante, nitrante e nucleofilico formado in vivo pela reação difusional do radical ânion superóxido com óxido nítrico, cuja produção exacerbada em situações de estresse oxidativo e nitrosativo pode resultar em danos a biomoléculas e estruturas sub-celulares. Por outro lado, vários compostos carbonílicos reativos tais como acroleína e compostos α-dicarbonílicos são descritos como citotóxicos e genotóxicos, pois reagem com biomoléculas aminadas resultando em perda de funções nativas, situação esta denominada de ëstresse carbonílico\". Dentre os metabólitos α-dicarbonílicos, altamente suscetíveis a adições nucleofílicas, destacam-se o biacetilo, derivado do metabolismo hepático de etanol e contaminante de alimentos, e metilglioxal e glioxal, ambos catabólitos de glicose, proteínas e lipídios acumulados em doenças relacionadas ao envelhecimento. Neste trabalho, observou-se que, em tampão fosfato normalmente aerado de pH próximo à neutralidade, (i) estes três compostos sofrem adição nucleofílica de peroxinitrito com constantes de velocidade de segunda ordem uma a três ordens de grandeza acima dos valores relatados para compostos monocarbonílicos (k2 ≈ 4-100 M-1s-1); (ii) os sistemas biacetilo ou metilglioxal/peroxinitrito consomem o oxigênio dissolvido com produção de acetato ou acetato e formiato, respectivamente, via radical acetila capaz de acetilar histidina, lisina e 2\'-desoxiguanosina se adicionados à mistura reacional; e (iii) o sistema glioxal/peroxinitrito gera sucessivamente radical formila e radical formilhidroperoxila, cujo desproporcionamento a formiato e gás carbônico é acompanhado da emissão de luz no infra-vermelho próximo (λmax = 1270 nm), atribuída a oxigênio molecular no estado singlete (O21Δg) (Reação de Russell). Estes estudos evidenciam que a reação de metabólitos α-dicarbonílicos com peroxinitrito gera radicais livres e embasam a hipótese de que possam contribuir para a acetilação radicalar, não-enzimática intracelular, de proteínas (epigenética) e DNA, portanto potencialmente implicadas na fisiologia e patologia do envelhecimento e desordens metabólicas, nas quais a participação de espécies reativas de oxigênio, nitrogênio e compostos carbonílicos foi relatada. Deve-se ainda notar que a descoberta de acetilação radicalar de biomoléculas por metabólitos α-dicarbonilicos e peroxinitrito prepara o caminho para a identificação de novas reações químicas de biomoléculas, não catalisadas por enzimas, que possam eventualmente revelar novos biomarcadores teciduais em doenças adquiridas e inatas.

Peroxynitrite is a strong biological oxidant, nitrating and nucleophilic agent, formed by the diffusion-controlled reaction of the superoxide anion radical with nitric oxide, whose exacerbated production in oxidative and nitrosative stress leads to chemical damage to biomolecules and sub-cellular structures. On the other hand, various reactive carbonyl compounds like acrolein and α-dicarbonyls are reportedly cytotoxic and genotoxic for…

Advisors/Committee Members: Bechara, Etelvino Jose Henriques.

Subjects/Keywords: Acetyl radical; Biacetilo; Biacetyl; Estresse oxidativo; Glioxal; Glyoxal; Methylglyoxal; Metilglioxal; Oxidative stress; Oxigênio singlete; Peroxinitrito; Peroxynitrite; Radical acetila; Singlet oxyge

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Massari Filho, J. (2014). Mecanismos da reação de metabólitos α-dicarbonílicos com peroxinitrito: geração de radicais livres e oxigênio singlete. Possíveis implicações biológicas. (Doctoral Dissertation). University of São Paulo. Retrieved from http://www.teses.usp.br/teses/disponiveis/46/46136/tde-02102014-081602/ ;

Chicago Manual of Style (16^th Edition):

Massari Filho, Júlio. “Mecanismos da reação de metabólitos α-dicarbonílicos com peroxinitrito: geração de radicais livres e oxigênio singlete. Possíveis implicações biológicas.” 2014. Doctoral Dissertation, University of São Paulo. Accessed January 18, 2021. http://www.teses.usp.br/teses/disponiveis/46/46136/tde-02102014-081602/ ;.

MLA Handbook (7^th Edition):

Massari Filho, Júlio. “Mecanismos da reação de metabólitos α-dicarbonílicos com peroxinitrito: geração de radicais livres e oxigênio singlete. Possíveis implicações biológicas.” 2014. Web. 18 Jan 2021.

Vancouver:

Massari Filho J. Mecanismos da reação de metabólitos α-dicarbonílicos com peroxinitrito: geração de radicais livres e oxigênio singlete. Possíveis implicações biológicas. [Internet] [Doctoral dissertation]. University of São Paulo; 2014. [cited 2021 Jan 18]. Available from: http://www.teses.usp.br/teses/disponiveis/46/46136/tde-02102014-081602/ ;.

Council of Science Editors:

Massari Filho J. Mecanismos da reação de metabólitos α-dicarbonílicos com peroxinitrito: geração de radicais livres e oxigênio singlete. Possíveis implicações biológicas. [Doctoral Dissertation]. University of São Paulo; 2014. Available from: http://www.teses.usp.br/teses/disponiveis/46/46136/tde-02102014-081602/ ;

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