subject:(Perfusion)

University of Arizona

1. Dimeo, Nicholas. Adult Renal Near Infrared Spectroscopy and Oxygen Delivery During CPB: Correlations to Prevent Acute Kidney Injury .

Degree: 2017, University of Arizona

URL: http://hdl.handle.net/10150/624114

► A major complication associated with cardiopulmonary bypass (CPB) is acute kidney injury (AKI), with around 30% of patients experiencing some sort of renal insult (31).… (more)

▼ A major complication associated with cardiopulmonary bypass (CPB) is acute kidney injury (AKI), with around 30% of patients experiencing some sort of renal insult (31). Kidney performance is strongly linked to cardiac performance, so perfusionists can play a major role in implementing strategies to reduce the incidence of AKI. The use of Near Infrared Spectroscopy (NIRS) has been validated for the use of cerebral oximetry for both pediatric and adult patients, unlike somatic monitoring where only pediatric randomized control trials have proven to be successful. Since the distance from sensor to organ for adults is greater than 1.4 centimeters, there are not any studies to correlate perfusion parameters to adult renal oximetry. The primary goal of this pilot study was to correlate renal oximetry values to pre-established perfusion outcome markers that are routinely measured during CPB. In this way, renal NIRS may be used as a real time trending device to help prevent AKI. The INVOS™ system was used for both cerebral and renal NIRS monitoring. Renal oximetry pads were placed between the 11th-12th intercostal spaces and the most accurate baseline rSO2 (regional oxygen saturation) level was obtained before sedating the patients. Baseline variables obtained were: age, weight, height, body surface area, history of diabetes, ejection fraction, creatinine, hemoglobin, hematocrit, cerebral oximetry, renal oximetry, and lactate values. Operative variables obtained were: hemoglobin, mean arterial pressure, pump flow, cardiac index, cerebral oximetry, renal oximetry, lactate, temperature, venous oxygen saturation, and oxygen delivery. A multivariable statistical analysis model was used to correlate the data. The results showed the strongest statistical correlations of renal oximetry with hemoglobin (p<0.01, p=0.01), cardiac index (p<0.0001, p<0.01), and oxygen delivery (p<0.0001, p<0.0001). The higher these variables were, the higher the renal oximetry values and vice versa. The changes in oxygen delivery were correlated to the changes in renal oximetry values. Specifically as the DO2 increases 1.15 mL/min1/m2 (p<0.01), the percent change in the left renal oximetry increases 1%. As for the right renal, when the DO2 increases 0.94 mL/min1/m2 (p<0.01), the percent change in the right oximetry increases 1%. A renal oximetry value with a decrease of more than 20% from pre-operative baseline is associated with a significantly lower DO2 than renal oximetry values without a decrease more than 20% from pre-operative (p=0.01). The DO2 difference was calculated at 21.97 ml/min1/m2. There is a direct correlation between oxygen delivery values and renal oximetry saturation values. In conclusion, this pilot observational study has shown the INVOS™ system to be a valuable real-time trending device for renal oximetry saturation values with perfusion parameters to help prevent or reduce acute kidney injuries for cardiopulmonary bypass patients. Advisors/Committee Members: Wong, Raymond K (advisor), Wong, Raymond K. (committeemember), Khalpey, Zain I. (committeemember), Paidy, Samata R. (committeemember).

Subjects/Keywords: Perfusion Science

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APA (6^th Edition):

Dimeo, N. (2017). Adult Renal Near Infrared Spectroscopy and Oxygen Delivery During CPB: Correlations to Prevent Acute Kidney Injury . (Masters Thesis). University of Arizona. Retrieved from http://hdl.handle.net/10150/624114

Chicago Manual of Style (16^th Edition):

Dimeo, Nicholas. “Adult Renal Near Infrared Spectroscopy and Oxygen Delivery During CPB: Correlations to Prevent Acute Kidney Injury .” 2017. Masters Thesis, University of Arizona. Accessed April 11, 2021. http://hdl.handle.net/10150/624114.

MLA Handbook (7^th Edition):

Dimeo, Nicholas. “Adult Renal Near Infrared Spectroscopy and Oxygen Delivery During CPB: Correlations to Prevent Acute Kidney Injury .” 2017. Web. 11 Apr 2021.

Vancouver:

Dimeo N. Adult Renal Near Infrared Spectroscopy and Oxygen Delivery During CPB: Correlations to Prevent Acute Kidney Injury . [Internet] [Masters thesis]. University of Arizona; 2017. [cited 2021 Apr 11]. Available from: http://hdl.handle.net/10150/624114.

Council of Science Editors:

Dimeo N. Adult Renal Near Infrared Spectroscopy and Oxygen Delivery During CPB: Correlations to Prevent Acute Kidney Injury . [Masters Thesis]. University of Arizona; 2017. Available from: http://hdl.handle.net/10150/624114

University of Adelaide

2. Kuan, Kean Guan. Extracorporeal normothermic pancreas perfusion.

Degree: 2016, University of Adelaide

URL: http://hdl.handle.net/2440/102708

► Pancreas and islet transplantation are important treatment options for insulin dependent diabetes. However, one of the main challenges in pancreas and islet transplantation lies in… (more)

Subjects/Keywords: extracorporeal perfusion; ex-vivo perfusion; normothermic perfusion; pancreas

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APA (6^th Edition):

Kuan, K. G. (2016). Extracorporeal normothermic pancreas perfusion. (Thesis). University of Adelaide. Retrieved from http://hdl.handle.net/2440/102708

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Kuan, Kean Guan. “Extracorporeal normothermic pancreas perfusion.” 2016. Thesis, University of Adelaide. Accessed April 11, 2021. http://hdl.handle.net/2440/102708.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Kuan, Kean Guan. “Extracorporeal normothermic pancreas perfusion.” 2016. Web. 11 Apr 2021.

Vancouver:

Kuan KG. Extracorporeal normothermic pancreas perfusion. [Internet] [Thesis]. University of Adelaide; 2016. [cited 2021 Apr 11]. Available from: http://hdl.handle.net/2440/102708.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Kuan KG. Extracorporeal normothermic pancreas perfusion. [Thesis]. University of Adelaide; 2016. Available from: http://hdl.handle.net/2440/102708

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Western Ontario

3. Marants, Raanan. Exploring the Effects of Hemodialysis on Renal and Hepatic Blood Flow and Function using CT Perfusion Imaging.

Degree: 2020, University of Western Ontario

URL: https://ir.lib.uwo.ca/etd/7081

► Hemodialysis (HD) is the most common form of renal replacement therapy for end-stage renal disease. However, patients develop complications that are driven by HD-induced circulatory… (more)

▼ Hemodialysis (HD) is the most common form of renal replacement therapy for end-stage renal disease. However, patients develop complications that are driven by HD-induced circulatory stress from rapidly removing large fluid volumes during HD, making various vascular beds vulnerable to ischemia. By assessing how HD-induced circulatory stress affects different organs, it may be possible to characterize the mechanisms behind these complications and evaluate therapeutic interventions. This thesis aims to explore how HD affects renal and hepatic blood flow and function using CT perfusion imaging. For this work, patients received either standard or cooled HD first in a two-visit, crossover study design, where imaging was performed before, during and after each HD session. Residual renal function is linked to improved clinical outcomes, yet characteristically declines upon HD initiation. In the first thesis project, we determined that renal perfusion deceases during HD, which could be an early manifestation of HD-mediated residual renal function loss. Although the liver normally clears endotoxin, increased circulating endotoxin levels have been found in HD patients. In the second thesis project, we showed that concurrent hepatic perfusion redistribution and decreased liver function during HD are likely responsible for increased circulating toxin levels. Dialysate cooling is a low-cost, feasible intervention that ameliorates HD-induced circulatory stress. In the first and second thesis projects, we found that cooling trended towards mitigating the drop in renal perfusion during HD and ameliorating the changes in liver perfusion and function during HD. If it were possible to accurately assess glomerular filtration rate (GFR) in HD patients, HD prescriptions could be adjusted in accordance with residual renal function to preserve remaining function. In the third thesis project, we extended the CT perfusion technique to measure GFR in HD patients, yielding physiologically realistic GFR values, thus demonstrating the feasibility of this approach in terms of reliability and accuracy. These findings help explain residual renal function loss and endotoxemia in HD patients, and showcases the protective potential of dialysate cooling. In addition, this work demonstrates the benefit of using CT perfusion as a functional imaging technique to further characterize and evaluate therapies for end-stage renal disease pathologies.

Subjects/Keywords: hemodialysis; computed tomography perfusion; renal perfusion; hepatic perfusion; glomerular filtration rate; dialysate cooling; Medical Biophysics

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APA (6^th Edition):

Marants, R. (2020). Exploring the Effects of Hemodialysis on Renal and Hepatic Blood Flow and Function using CT Perfusion Imaging. (Thesis). University of Western Ontario. Retrieved from https://ir.lib.uwo.ca/etd/7081

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Marants, Raanan. “Exploring the Effects of Hemodialysis on Renal and Hepatic Blood Flow and Function using CT Perfusion Imaging.” 2020. Thesis, University of Western Ontario. Accessed April 11, 2021. https://ir.lib.uwo.ca/etd/7081.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Marants, Raanan. “Exploring the Effects of Hemodialysis on Renal and Hepatic Blood Flow and Function using CT Perfusion Imaging.” 2020. Web. 11 Apr 2021.

Vancouver:

Marants R. Exploring the Effects of Hemodialysis on Renal and Hepatic Blood Flow and Function using CT Perfusion Imaging. [Internet] [Thesis]. University of Western Ontario; 2020. [cited 2021 Apr 11]. Available from: https://ir.lib.uwo.ca/etd/7081.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Marants R. Exploring the Effects of Hemodialysis on Renal and Hepatic Blood Flow and Function using CT Perfusion Imaging. [Thesis]. University of Western Ontario; 2020. Available from: https://ir.lib.uwo.ca/etd/7081

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Louisville

4. Buller, Mitchell J. Development of a physiologic ex vivo vessel perfusion system.

Degree: M. Eng., 2013, University of Louisville

URL: 10.18297/etd/177 ; https://ir.library.louisville.edu/etd/177

► Introduction: Over time, continuous flow ventricular assist devices (VADs) have become the primary form of implanted mechanical circulatory support (MCS) due to their smaller size,… (more)

▼ Introduction: Over time, continuous flow ventricular assist devices (VADs) have become the primary form of implanted mechanical circulatory support (MCS) due to their smaller size, higher energy efficiency, longer durability, and fewer LVAD-related complications when compared to pulsatile flow VADs. However, continuous and pulsatile flows may elicit different cellular and tissue response, particularly in the arterial vasculature, which could have a profound impact on the future operation of MCS devices. Therefore, a unique ex vivo perfusion system integrated with a mock adult circulatory system was design to study the impact of VAD-generated flow patterns on vascular function. Methods: The benefits of a mock circulatory loop and an ex vivo perfusion system were combined by designing and integrating a vessel perfusion chamber to an adult-sized mock circulatory loop as a parallel flow branch distal to VAD outflow. Testing was conducted using a mock over several physiologic conditions (normal, heart failure, and hypertension) and at various levels of VAD flow. The system was integrated into an incubator to allow for control of pH and temperature in future studies and fitted with a vessel for feasibility testing. Data was collected using a custom Labview program and analyzed using the HEART program, an automated beat-to-beat cardiovascular analysis program based in Matlab. Results: The chamber was successfully fabricated and installed in the mock circulatory system, allowing for perfusion and longitudinal stretching of bovine carotid arteries. The waveforms and values for pressures and flows created in the mock loop were similar to physiologic values under each tested condition. Under normal hemodynamic conditions (CO = 4.5 L/min, MAP = 91 mmHg) perfusion chamber flow was 0.51 L/min, while under HF conditions (CO = 3.3 L/min, MAP = 81 mmHg) it was reduced to 0.18 L/min, which are representative of in vivo carotid artery hemodynamics. Due to physiologic preloads and afterloads, VAD performance was as would be expected in clinical application. The system was found to be sufficient for future testing with bovine carotid arteries and extended perfusion times (>24 hours). Conclusions: This study resulted in an ex vivo vessel perfusion system that can successfully expose bovine carotid arteries to physiologic and VAD-specific hemodynamic waveforms. The ability to combine the mock ventricle with clinically implanted VADs makes this system both unique and clinically relevant for studying the effects of continuous versus pulsatile flow on the peripheral vasculature. Advisors/Committee Members: Koenig, Steven Christopher.

Subjects/Keywords: Ex vivo perfusion; Vessel perfusion; Physiologic perfusion; Mock circulation; Pulsatile flow; Carotid artery

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APA (6^th Edition):

Buller, M. J. (2013). Development of a physiologic ex vivo vessel perfusion system. (Masters Thesis). University of Louisville. Retrieved from 10.18297/etd/177 ; https://ir.library.louisville.edu/etd/177

Chicago Manual of Style (16^th Edition):

Buller, Mitchell J. “Development of a physiologic ex vivo vessel perfusion system.” 2013. Masters Thesis, University of Louisville. Accessed April 11, 2021. 10.18297/etd/177 ; https://ir.library.louisville.edu/etd/177.

MLA Handbook (7^th Edition):

Buller, Mitchell J. “Development of a physiologic ex vivo vessel perfusion system.” 2013. Web. 11 Apr 2021.

Vancouver:

Buller MJ. Development of a physiologic ex vivo vessel perfusion system. [Internet] [Masters thesis]. University of Louisville; 2013. [cited 2021 Apr 11]. Available from: 10.18297/etd/177 ; https://ir.library.louisville.edu/etd/177.

Council of Science Editors:

Buller MJ. Development of a physiologic ex vivo vessel perfusion system. [Masters Thesis]. University of Louisville; 2013. Available from: 10.18297/etd/177 ; https://ir.library.louisville.edu/etd/177

Ruhr Universität Bochum

5. Sarigiannis, Konstantinos. Wert der klinischen Symptome und D-Dimere für die Verdachtsdiagnose "Lungenembolie" in der Computertomographie.

Degree: 2014, Ruhr Universität Bochum

URL: http://nbn-resolving.de/urn/resolver.pl?urn=urn:nbn:de:hbz:294-42235

► Methode und Patienten: Es wurden 278 Patienten im Zeitraum vom 01.01.2009 bis zum 31.12.2009 unter der Verdachtsdiagnose "Lungenembolie" im CT untersucht. Dabei handelte es sich… (more)

Subjects/Keywords: Lungenembolie; Dimere; Computertomographie; Symptombildung; Perfusion

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APA (6^th Edition):

Sarigiannis, K. (2014). Wert der klinischen Symptome und D-Dimere für die Verdachtsdiagnose "Lungenembolie" in der Computertomographie. (Thesis). Ruhr Universität Bochum. Retrieved from http://nbn-resolving.de/urn/resolver.pl?urn=urn:nbn:de:hbz:294-42235

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Sarigiannis, Konstantinos. “Wert der klinischen Symptome und D-Dimere für die Verdachtsdiagnose "Lungenembolie" in der Computertomographie.” 2014. Thesis, Ruhr Universität Bochum. Accessed April 11, 2021. http://nbn-resolving.de/urn/resolver.pl?urn=urn:nbn:de:hbz:294-42235.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Sarigiannis, Konstantinos. “Wert der klinischen Symptome und D-Dimere für die Verdachtsdiagnose "Lungenembolie" in der Computertomographie.” 2014. Web. 11 Apr 2021.

Vancouver:

Sarigiannis K. Wert der klinischen Symptome und D-Dimere für die Verdachtsdiagnose "Lungenembolie" in der Computertomographie. [Internet] [Thesis]. Ruhr Universität Bochum; 2014. [cited 2021 Apr 11]. Available from: http://nbn-resolving.de/urn/resolver.pl?urn=urn:nbn:de:hbz:294-42235.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Sarigiannis K. Wert der klinischen Symptome und D-Dimere für die Verdachtsdiagnose "Lungenembolie" in der Computertomographie. [Thesis]. Ruhr Universität Bochum; 2014. Available from: http://nbn-resolving.de/urn/resolver.pl?urn=urn:nbn:de:hbz:294-42235

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Utah

6. Kennedy, James Paul. A cellularized biomaterial model of cardiac fibroblasts for evaluation of fibroblast induced changes in stiffness.

Degree: PhD, Bioengineering, 2013, University of Utah

URL: http://content.lib.utah.edu/cdm/singleitem/collection/etd3/id/2300/rec/15

► Chronic heart failure (CHF) is a life-altering long-term condition that contributesa substantial burden to our healthcare system. It is caused by a maladaptive remodeling ofthe… (more)

▼ Chronic heart failure (CHF) is a life-altering long-term condition that contributesa substantial burden to our healthcare system. It is caused by a maladaptive remodeling ofthe heart mediated through fibroblast synthesis, degradation, and modification ofextracellular matrix (ECM). It is currently managed through pharmacologic interventionor medical device treatment, but can be reversed only through heart transplantation. Celltherapy is a new approach to treating CHF that promises to prevent and potentiallyreverse cardiac remodeling through interaction with cardiac fibroblasts. Adherent bonemarrow derived stem cells (MSC) are one of the most promising candidates for use incell therapies. The major challenge hindering standard clinical application of MSCtherapy is limited understanding of how MSC interact with heart cells to reverseremodeling. Numerous techniques are available to harvest, isolate, and modify MSC,however these techniques are believed to influence the efficacy of the treatment. Currenttechniques for evaluating efficacy of MSC treatments are either prohibitively difficult orsignificantly limited in their ability to assess functional changes. Establishing an in vitroplatform for evaluating the influence of MSC coculture on performance characteristics ofcardiac fibroblasts is a logical and efficient step prior to successful clinicalimplementation of MSC therapy. The objective of this research was to develop a threedimensional(3D) tissue model that allows investigation of the underlying mechanismresponsible for MSC mediated cardiac regeneration. The three phases of this workincluded: (1) development of a biomaterial substrate capable of sustaining fibroblastattachment, proliferation, and alignment, (2) development of a culture platform andseeding techniques capable of providing sufficient mass transport to sustain a relativelythick 3D scaffold populated with both fibroblasts and MSC (3) application of thesubstrate and culture platform to evaluate changes in mechanical properties and celldistribution resulting from MSC coculture with cardiac fibroblasts.

Subjects/Keywords: bioreactor; cardiac fibroblasts; perfusion; scaffold

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APA (6^th Edition):

Kennedy, J. P. (2013). A cellularized biomaterial model of cardiac fibroblasts for evaluation of fibroblast induced changes in stiffness. (Doctoral Dissertation). University of Utah. Retrieved from http://content.lib.utah.edu/cdm/singleitem/collection/etd3/id/2300/rec/15

Chicago Manual of Style (16^th Edition):

Kennedy, James Paul. “A cellularized biomaterial model of cardiac fibroblasts for evaluation of fibroblast induced changes in stiffness.” 2013. Doctoral Dissertation, University of Utah. Accessed April 11, 2021. http://content.lib.utah.edu/cdm/singleitem/collection/etd3/id/2300/rec/15.

MLA Handbook (7^th Edition):

Kennedy, James Paul. “A cellularized biomaterial model of cardiac fibroblasts for evaluation of fibroblast induced changes in stiffness.” 2013. Web. 11 Apr 2021.

Vancouver:

Kennedy JP. A cellularized biomaterial model of cardiac fibroblasts for evaluation of fibroblast induced changes in stiffness. [Internet] [Doctoral dissertation]. University of Utah; 2013. [cited 2021 Apr 11]. Available from: http://content.lib.utah.edu/cdm/singleitem/collection/etd3/id/2300/rec/15.

Council of Science Editors:

Kennedy JP. A cellularized biomaterial model of cardiac fibroblasts for evaluation of fibroblast induced changes in stiffness. [Doctoral Dissertation]. University of Utah; 2013. Available from: http://content.lib.utah.edu/cdm/singleitem/collection/etd3/id/2300/rec/15

7. Mora Álvarez, María G. Arterial Spin Labeling (ASL) Imaging of Cerebral Perfusion at High Magnetic Fields.

Degree: MS, Department of Biomedical Engineering, 2016, University of Alberta

URL: https://era.library.ualberta.ca/files/c2f75r823n

► Arterial spin labeling (ASL) is a magnetic resonance imaging (MRI) noninvasive method, capable of measuring perfusion, i.e. blood flow, with blood as an intrinsic contrast.… (more)

Subjects/Keywords: ASL; MRI; Brain; Perfusion

11 more images…

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APA (6^th Edition):

Mora Álvarez, M. G. (2016). Arterial Spin Labeling (ASL) Imaging of Cerebral Perfusion at High Magnetic Fields. (Masters Thesis). University of Alberta. Retrieved from https://era.library.ualberta.ca/files/c2f75r823n

Chicago Manual of Style (16^th Edition):

Mora Álvarez, María G. “Arterial Spin Labeling (ASL) Imaging of Cerebral Perfusion at High Magnetic Fields.” 2016. Masters Thesis, University of Alberta. Accessed April 11, 2021. https://era.library.ualberta.ca/files/c2f75r823n.

MLA Handbook (7^th Edition):

Mora Álvarez, María G. “Arterial Spin Labeling (ASL) Imaging of Cerebral Perfusion at High Magnetic Fields.” 2016. Web. 11 Apr 2021.

Vancouver:

Mora Álvarez MG. Arterial Spin Labeling (ASL) Imaging of Cerebral Perfusion at High Magnetic Fields. [Internet] [Masters thesis]. University of Alberta; 2016. [cited 2021 Apr 11]. Available from: https://era.library.ualberta.ca/files/c2f75r823n.

Council of Science Editors:

Mora Álvarez MG. Arterial Spin Labeling (ASL) Imaging of Cerebral Perfusion at High Magnetic Fields. [Masters Thesis]. University of Alberta; 2016. Available from: https://era.library.ualberta.ca/files/c2f75r823n

Cornell University

8. Xu, Bo. Interrogating Tissue Perfusion And Oxygenation Using Dynamic Magnetic Resonance Imaging And Quantitative Susceptibility Mapping.

Degree: PhD, Biomedical Engineering, 2014, Cornell University

URL: http://hdl.handle.net/1813/36055

► Magnetic resonance imaging (MRI) is a widely used non-invasive imaging technique, with rich contrast for interrogation of tissue physiology and pathology. MRI can be used… (more)

Subjects/Keywords: Dynamic MRI; Perfusion MRI; QSM

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APA (6^th Edition):

Xu, B. (2014). Interrogating Tissue Perfusion And Oxygenation Using Dynamic Magnetic Resonance Imaging And Quantitative Susceptibility Mapping. (Doctoral Dissertation). Cornell University. Retrieved from http://hdl.handle.net/1813/36055

Chicago Manual of Style (16^th Edition):

Xu, Bo. “Interrogating Tissue Perfusion And Oxygenation Using Dynamic Magnetic Resonance Imaging And Quantitative Susceptibility Mapping.” 2014. Doctoral Dissertation, Cornell University. Accessed April 11, 2021. http://hdl.handle.net/1813/36055.

MLA Handbook (7^th Edition):

Xu, Bo. “Interrogating Tissue Perfusion And Oxygenation Using Dynamic Magnetic Resonance Imaging And Quantitative Susceptibility Mapping.” 2014. Web. 11 Apr 2021.

Vancouver:

Xu B. Interrogating Tissue Perfusion And Oxygenation Using Dynamic Magnetic Resonance Imaging And Quantitative Susceptibility Mapping. [Internet] [Doctoral dissertation]. Cornell University; 2014. [cited 2021 Apr 11]. Available from: http://hdl.handle.net/1813/36055.

Council of Science Editors:

Xu B. Interrogating Tissue Perfusion And Oxygenation Using Dynamic Magnetic Resonance Imaging And Quantitative Susceptibility Mapping. [Doctoral Dissertation]. Cornell University; 2014. Available from: http://hdl.handle.net/1813/36055

Vanderbilt University

9. Watchmaker, Jennifer Morgan. Intracranial Hemodynamic Compensation Mechanisms in Patients with Cerebrovascular Disease.

Degree: PhD, Chemical and Physical Biology, 2017, Vanderbilt University

URL: http://hdl.handle.net/1803/14079

► Cerebrovascular disease (CVD) encompasses conditions that affect the blood supply to the brain. CVD results in hemodynamic impairment, and when the brain can no longer… (more)

Subjects/Keywords: MRI; perfusion; stroke; cerebrovascular disease

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APA (6^th Edition):

Watchmaker, J. M. (2017). Intracranial Hemodynamic Compensation Mechanisms in Patients with Cerebrovascular Disease. (Doctoral Dissertation). Vanderbilt University. Retrieved from http://hdl.handle.net/1803/14079

Chicago Manual of Style (16^th Edition):

Watchmaker, Jennifer Morgan. “Intracranial Hemodynamic Compensation Mechanisms in Patients with Cerebrovascular Disease.” 2017. Doctoral Dissertation, Vanderbilt University. Accessed April 11, 2021. http://hdl.handle.net/1803/14079.

MLA Handbook (7^th Edition):

Watchmaker, Jennifer Morgan. “Intracranial Hemodynamic Compensation Mechanisms in Patients with Cerebrovascular Disease.” 2017. Web. 11 Apr 2021.

Vancouver:

Watchmaker JM. Intracranial Hemodynamic Compensation Mechanisms in Patients with Cerebrovascular Disease. [Internet] [Doctoral dissertation]. Vanderbilt University; 2017. [cited 2021 Apr 11]. Available from: http://hdl.handle.net/1803/14079.

Council of Science Editors:

Watchmaker JM. Intracranial Hemodynamic Compensation Mechanisms in Patients with Cerebrovascular Disease. [Doctoral Dissertation]. Vanderbilt University; 2017. Available from: http://hdl.handle.net/1803/14079

Harvard University

10. Brush, Benjamin. Evaluation of the Change in Vascular Tone Following Brachial Plexus Nerve Block Using Amplification of Video From a Mobile Device.

Degree: Doctor of Medicine, 2016, Harvard University

URL: http://nrs.harvard.edu/urn-3:HUL.InstRepos:40620277

►

Purpose: To evaluate the efficacy of using an iPhone as a pulse plethysmograph in detecting changes in cutaneous perfusion and vascular tone caused by regional… (more)

Subjects/Keywords: perfusion; nerve block; iPhone; plethysmograph

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APA (6^th Edition):

Brush, B. (2016). Evaluation of the Change in Vascular Tone Following Brachial Plexus Nerve Block Using Amplification of Video From a Mobile Device. (Doctoral Dissertation). Harvard University. Retrieved from http://nrs.harvard.edu/urn-3:HUL.InstRepos:40620277

Chicago Manual of Style (16^th Edition):

Brush, Benjamin. “Evaluation of the Change in Vascular Tone Following Brachial Plexus Nerve Block Using Amplification of Video From a Mobile Device.” 2016. Doctoral Dissertation, Harvard University. Accessed April 11, 2021. http://nrs.harvard.edu/urn-3:HUL.InstRepos:40620277.

MLA Handbook (7^th Edition):

Brush, Benjamin. “Evaluation of the Change in Vascular Tone Following Brachial Plexus Nerve Block Using Amplification of Video From a Mobile Device.” 2016. Web. 11 Apr 2021.

Vancouver:

Brush B. Evaluation of the Change in Vascular Tone Following Brachial Plexus Nerve Block Using Amplification of Video From a Mobile Device. [Internet] [Doctoral dissertation]. Harvard University; 2016. [cited 2021 Apr 11]. Available from: http://nrs.harvard.edu/urn-3:HUL.InstRepos:40620277.

Council of Science Editors:

Brush B. Evaluation of the Change in Vascular Tone Following Brachial Plexus Nerve Block Using Amplification of Video From a Mobile Device. [Doctoral Dissertation]. Harvard University; 2016. Available from: http://nrs.harvard.edu/urn-3:HUL.InstRepos:40620277

University of Toronto

11. Peladeau-Pigeon, Melanie. Simulation of Perfusion Flow Dynamics for Contrast Enhanced Imaging.

Degree: 2012, University of Toronto

URL: http://hdl.handle.net/1807/33495

►

Dynamic Contrast Enhanced Computed Tomography is an imaging tool that aids in evaluating functional characteristics in different stages of disease assessment: diagnostic, treatment effectiveness and… (more)

Subjects/Keywords: CT; DCE-CT; Perfusion; 0541

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APA (6^th Edition):

Peladeau-Pigeon, M. (2012). Simulation of Perfusion Flow Dynamics for Contrast Enhanced Imaging. (Masters Thesis). University of Toronto. Retrieved from http://hdl.handle.net/1807/33495

Chicago Manual of Style (16^th Edition):

Peladeau-Pigeon, Melanie. “Simulation of Perfusion Flow Dynamics for Contrast Enhanced Imaging.” 2012. Masters Thesis, University of Toronto. Accessed April 11, 2021. http://hdl.handle.net/1807/33495.

MLA Handbook (7^th Edition):

Peladeau-Pigeon, Melanie. “Simulation of Perfusion Flow Dynamics for Contrast Enhanced Imaging.” 2012. Web. 11 Apr 2021.

Vancouver:

Peladeau-Pigeon M. Simulation of Perfusion Flow Dynamics for Contrast Enhanced Imaging. [Internet] [Masters thesis]. University of Toronto; 2012. [cited 2021 Apr 11]. Available from: http://hdl.handle.net/1807/33495.

Council of Science Editors:

Peladeau-Pigeon M. Simulation of Perfusion Flow Dynamics for Contrast Enhanced Imaging. [Masters Thesis]. University of Toronto; 2012. Available from: http://hdl.handle.net/1807/33495

Syracuse University

12. Sawyer, Stephen William. STRUCTURALLY SUPPORTED CELL-LADEN SCAFFOLDS FOR BONE TISSUE REGENERATION.

Degree: PhD, Biomedical and Chemical Engineering, 2018, Syracuse University

URL: https://surface.syr.edu/etd/978

► Due to challenges associated with current clinical techniques used to treat bone defects, there has been an increased focus on finding a tissue engineered… (more)

▼ Due to challenges associated with current clinical techniques used to treat bone defects, there has been an increased focus on finding a tissue engineered solution. However, while great progress in this field has been achieved, researchers have yet to suitably combine the proper biological and structural environments needed to serve as a complete bone tissue substitute that is comparable to modern clinical solutions. To achieve the goal of creating a model bone tissue substitute which could eventually serve as a viable therapy for bone trauma, be it caused by congenital medical conditions, age related diseases or high impact forces, three areas of the engineered construct architecture and composition were identified and studied in a successive fashion. First, a soft, biocompatible matrix within which cells could be encapsulated was studied, followed by an investigation on how to combine the soft matrix with a 3D printed structural frame. Finally, user-defined perfusable vasculature was added to the soft matrix in order to create a model bone tissue engineering construct capable of possible in vivo implantation. In Chapter 2 of this work, osteoblast-like human osteosarcoma cells (Saos-2) were encapsulated within gelatin methacrylate (GelMA) hydrogels and the effect of the hydrogel density on cellular morphology and mineralization was investigated. It was found that the less dense hydrogels allowed for increased cell viability and spreading, while the denser gels appeared to encourage more mineral deposition on the construct periphery. Building upon Chapter 2, Chapter 3 focused on the 3D printing of polycaprolactone (PCL) and composite PCL cages which could be combined with the soft GelMA matrices used for cellular encapsulation. It was found that while PCL and PCL composite cages could be reproducibly printed via a Makerbot 3D printer, the structural strengths did not surpass those of standard poly lactic acid (PLA) thermoplastic cages. Furthermore, it was demonstrated that the cell-laden GelMA hydrogels containing encapsulated Soas-2 cells could be incorporated with the 3D printed structures for potential bone tissue engineering applications. In Chapter 4, a simpler version of the cages produced in Chapter 3 were combined with sacrificial 3D printed polyvinyl alcohol (PVA) pipes and the dense cell-laden hydrogels investigated in Chapter 2 to create structurally supported, cell-laden hydrogel constructs. It was found that encapsulated cells could be stimulated to deposit mineral in the centers of the constructs via direct perfusion. However, in a larger version of the construct containing multiple pipes, mineralization was impeded due to diffusion issues caused by individual channel mineralization. Finally, in Chapter 5 future strategies to improve upon the structurally supported cell-laden hydrogels are discussed which would solve the issues found in Chapter 4. Additionally, potential in vivo applications for this system are… Advisors/Committee Members: Pranav Soman.

Subjects/Keywords: Bone; Perfusion; Tissue Engineering; Engineering

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Sawyer, S. W. (2018). STRUCTURALLY SUPPORTED CELL-LADEN SCAFFOLDS FOR BONE TISSUE REGENERATION. (Doctoral Dissertation). Syracuse University. Retrieved from https://surface.syr.edu/etd/978

Chicago Manual of Style (16^th Edition):

Sawyer, Stephen William. “STRUCTURALLY SUPPORTED CELL-LADEN SCAFFOLDS FOR BONE TISSUE REGENERATION.” 2018. Doctoral Dissertation, Syracuse University. Accessed April 11, 2021. https://surface.syr.edu/etd/978.

MLA Handbook (7^th Edition):

Sawyer, Stephen William. “STRUCTURALLY SUPPORTED CELL-LADEN SCAFFOLDS FOR BONE TISSUE REGENERATION.” 2018. Web. 11 Apr 2021.

Vancouver:

Sawyer SW. STRUCTURALLY SUPPORTED CELL-LADEN SCAFFOLDS FOR BONE TISSUE REGENERATION. [Internet] [Doctoral dissertation]. Syracuse University; 2018. [cited 2021 Apr 11]. Available from: https://surface.syr.edu/etd/978.

Council of Science Editors:

Sawyer SW. STRUCTURALLY SUPPORTED CELL-LADEN SCAFFOLDS FOR BONE TISSUE REGENERATION. [Doctoral Dissertation]. Syracuse University; 2018. Available from: https://surface.syr.edu/etd/978

13. Abou Samra, Waleed Ali. Effet du glaucome et des facteurs de risque de glaucome sur l'hémodynamique choroïdienne.

Degree: 2007, Université de Genève

URL: http://doc.rero.ch/record/7905

► Dans la présente étude, nous avons évalué quantitativement l'hémodynamique de la circulation choroïdienne subfovéolaire chez des patients atteints de glaucome à angle ouvert et des… (more)

Subjects/Keywords: perfusion pressure

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APA (6^th Edition):

Abou Samra, W. A. (2007). Effet du glaucome et des facteurs de risque de glaucome sur l'hémodynamique choroïdienne. (Thesis). Université de Genève. Retrieved from http://doc.rero.ch/record/7905

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Abou Samra, Waleed Ali. “Effet du glaucome et des facteurs de risque de glaucome sur l'hémodynamique choroïdienne.” 2007. Thesis, Université de Genève. Accessed April 11, 2021. http://doc.rero.ch/record/7905.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Abou Samra, Waleed Ali. “Effet du glaucome et des facteurs de risque de glaucome sur l'hémodynamique choroïdienne.” 2007. Web. 11 Apr 2021.

Vancouver:

Abou Samra WA. Effet du glaucome et des facteurs de risque de glaucome sur l'hémodynamique choroïdienne. [Internet] [Thesis]. Université de Genève; 2007. [cited 2021 Apr 11]. Available from: http://doc.rero.ch/record/7905.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Abou Samra WA. Effet du glaucome et des facteurs de risque de glaucome sur l'hémodynamique choroïdienne. [Thesis]. Université de Genève; 2007. Available from: http://doc.rero.ch/record/7905

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

14. Jalnefjord, Oscar. Intravoxel incoherent motion modeling - Optimization of acquisition, analysis and tumor tissue characterization.

Degree: 2018, University of Gothenburg / Göteborgs Universitet

URL: http://hdl.handle.net/2077/56355

► Intravoxel incoherent motion (IVIM) analysis provides a means to obtain information on diffusion and perfusion from a single MRI sequence. The measurements are completely noninvasive… (more)

▼ Intravoxel incoherent motion (IVIM) analysis provides a means to obtain information on diffusion and perfusion from a single MRI sequence. The measurements are completely noninvasive and the results have been shown to be of interest, for example, in oncological applications. Although the use of IVIM analysis has increased substantially the last decade, choice of acquisition parameters and analysis methods are still open questions. The aim of this thesis was to improve IVIM analysis by optimization of the image acquisition and parameter estimation methods, and to study the ability of IVIM parameters to be used for tumor tissue characterization. With standard model-fitting methods and data quality, IVIM parameter estimation uncertainty is typically high. However, several Bayesian approaches have been shown to improve parameter quality. In Paper I, these Bayesian approaches are compared using simulated data and data from a tumor mouse model. The results emphasize the impact of methodological choices, especially the prior distribution, at typical noise levels. Quick and robust IVIM examinations are important for clinical adoption, but consensus regarding methodology is lacking. To address this issue a framework for protocol optimization is presented in Paper III and a comparison of estimation methods was done in Paper II. To test the optimization framework, a protocol for liver examination was generated and tested on simulated data and data from healthy volunteers resulting in improved IVIM parameter quality. The compared estimation methods were evaluated on simulated data and data from patients with liver metastases with similar results for all methods, thereby making the computationally most effective method preferable. Studies of tumors using quantitative imaging methods such as IVIM often only extract an average parameter value from the entire tumor and may thus miss important information. Paper IV explores the ability of IVIM parameters to identify tumor subregions of functionally different status using clustering methods. The obtained subregions were found to have different proliferative status as derived from histological analysis. The work presented in this thesis has resulted in improved IVIM acquisition and analysis methods. It also shows that IVIM has the potential to provide insight into tumor physiology and be used as a noninvasive imaging biomarker.

Subjects/Keywords: IVIM; MRI; diffusion; perfusion; cancer

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APA (6^th Edition):

Jalnefjord, O. (2018). Intravoxel incoherent motion modeling - Optimization of acquisition, analysis and tumor tissue characterization. (Thesis). University of Gothenburg / Göteborgs Universitet. Retrieved from http://hdl.handle.net/2077/56355

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Jalnefjord, Oscar. “Intravoxel incoherent motion modeling - Optimization of acquisition, analysis and tumor tissue characterization.” 2018. Thesis, University of Gothenburg / Göteborgs Universitet. Accessed April 11, 2021. http://hdl.handle.net/2077/56355.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Jalnefjord, Oscar. “Intravoxel incoherent motion modeling - Optimization of acquisition, analysis and tumor tissue characterization.” 2018. Web. 11 Apr 2021.

Vancouver:

Jalnefjord O. Intravoxel incoherent motion modeling - Optimization of acquisition, analysis and tumor tissue characterization. [Internet] [Thesis]. University of Gothenburg / Göteborgs Universitet; 2018. [cited 2021 Apr 11]. Available from: http://hdl.handle.net/2077/56355.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Jalnefjord O. Intravoxel incoherent motion modeling - Optimization of acquisition, analysis and tumor tissue characterization. [Thesis]. University of Gothenburg / Göteborgs Universitet; 2018. Available from: http://hdl.handle.net/2077/56355

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Rice University

15. Santoro, Marco. Development of a High-Throughput 3D Tumor Model for Bone Sarcomas.

Degree: PhD, Engineering, 2016, Rice University

URL: http://hdl.handle.net/1911/95618

► Preclinical drug testing commonly relies on the use of two-dimensional (2D) cultures, which allow for rapid drug screening in vitro. However, 2D cultures are unable… (more)

▼ Preclinical drug testing commonly relies on the use of two-dimensional (2D) cultures, which allow for rapid drug screening in vitro. However, 2D cultures are unable to capture the complexity of the native three-dimensional (3D) tumor microenvironment, resulting in a lack of correspondence between preclinical data and clinical trial outcomes. The establishment of high-throughput 3D models able to describe distinctive aspects of the tumor niche would advance our understanding of tumor biology and would allow for drug testing in a physiologically relevant setup. Along this rationale, this thesis focuses on the development of a high-throughput 3D tumor model of bone sarcoma based on tissue-engineered polymeric scaffolds in combination with a flow perfusion bioreactor. First, we investigated the effects of flow perfusion on a 3D culture of Ewing sarcoma (ES) cells. We found that increasing levels of flow-derived shear stress promoted the secretion of insulin-like growth factor-1 (IGF-1) which, in turn, resulted in shear stress-dependent cell sensitivity to the IGF-1 receptor (IGF-1R) blockade, a central player in ES progression. We then leveraged these findings to culture ES cells on 3D-printed scaffolds under flow perfusion conditions. By designing 3D scaffolds with a defined porosity gradient, ES cells were exposed to a shear stress gradient that resulted in a gradient in cell response. In this way we sought to model variable levels of shear stress present within ES tumors due to intratumoral heterogeneity. In the final part of this thesis we investigated how the simultaneous presence of mesenchymal stem cells (MSCs) and flow perfusion affected drug sensitivity and phenotype of ES cells. We showed that the presence of MSCs within the coculture induces a progressive inhibition of cell growth and resistance to the IGF-1R blockade, highlighting the role of mechanically-sensitive mesodermal stroma on ES drug resistance. Overall, in this thesis we present a tissue-engineered tumor model that reliably mimics key features of the bone microenvironment, specifically the effects of biomechanical stimulation and of tumor-stroma interactions. The model hereby developed is amenable to further mechanistic studies on tumor biology and allows for a more accurate high-throughput screening of novel drug candidates. Advisors/Committee Members: Mikos, Antonios G (advisor), Ludwig, Joseph A (advisor).

Subjects/Keywords: tumor model; flow perfusion bioreactor

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Santoro, M. (2016). Development of a High-Throughput 3D Tumor Model for Bone Sarcomas. (Doctoral Dissertation). Rice University. Retrieved from http://hdl.handle.net/1911/95618

Chicago Manual of Style (16^th Edition):

Santoro, Marco. “Development of a High-Throughput 3D Tumor Model for Bone Sarcomas.” 2016. Doctoral Dissertation, Rice University. Accessed April 11, 2021. http://hdl.handle.net/1911/95618.

MLA Handbook (7^th Edition):

Santoro, Marco. “Development of a High-Throughput 3D Tumor Model for Bone Sarcomas.” 2016. Web. 11 Apr 2021.

Vancouver:

Santoro M. Development of a High-Throughput 3D Tumor Model for Bone Sarcomas. [Internet] [Doctoral dissertation]. Rice University; 2016. [cited 2021 Apr 11]. Available from: http://hdl.handle.net/1911/95618.

Council of Science Editors:

Santoro M. Development of a High-Throughput 3D Tumor Model for Bone Sarcomas. [Doctoral Dissertation]. Rice University; 2016. Available from: http://hdl.handle.net/1911/95618

University of Minnesota

16. Riesberg, Jeremiah. Creation of Perfusable Tissue Engineering Constructs through Biological Assembly.

Degree: PhD, Chemical Engineering, 2015, University of Minnesota

URL: http://hdl.handle.net/11299/191459

► One of the biggest barriers in creating large tissues is the lack of oxygen and nutrient transport required for cell growth and tissue development in… (more)

Subjects/Keywords: Perfusion Bioreactor; Tissue Engineering

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APA (6^th Edition):

Riesberg, J. (2015). Creation of Perfusable Tissue Engineering Constructs through Biological Assembly. (Doctoral Dissertation). University of Minnesota. Retrieved from http://hdl.handle.net/11299/191459

Chicago Manual of Style (16^th Edition):

Riesberg, Jeremiah. “Creation of Perfusable Tissue Engineering Constructs through Biological Assembly.” 2015. Doctoral Dissertation, University of Minnesota. Accessed April 11, 2021. http://hdl.handle.net/11299/191459.

MLA Handbook (7^th Edition):

Riesberg, Jeremiah. “Creation of Perfusable Tissue Engineering Constructs through Biological Assembly.” 2015. Web. 11 Apr 2021.

Vancouver:

Riesberg J. Creation of Perfusable Tissue Engineering Constructs through Biological Assembly. [Internet] [Doctoral dissertation]. University of Minnesota; 2015. [cited 2021 Apr 11]. Available from: http://hdl.handle.net/11299/191459.

Council of Science Editors:

Riesberg J. Creation of Perfusable Tissue Engineering Constructs through Biological Assembly. [Doctoral Dissertation]. University of Minnesota; 2015. Available from: http://hdl.handle.net/11299/191459

Queens University

17. Fleming, Sarah. Development of a Perfusion Bioreactor Strategy for Human Adipose-Derived Stem Cell Expansion .

Degree: Chemical Engineering, 2011, Queens University

URL: http://hdl.handle.net/1974/6867

► Developing an optimized growth environment for adipose-derived stems cells (ASCs) to obtain clinically useable cell quantities from relatively small tissue biopsies would significantly impact the… (more)

▼ Developing an optimized growth environment for adipose-derived stems cells (ASCs) to obtain clinically useable cell quantities from relatively small tissue biopsies would significantly impact the field of tissue engineering. To date, ASCs have been differentiated into adipose, bone, cartilage, smooth muscle, endothelial, skeletal muscle, nervous, and cardiac tissue. Therefore, ASCs have potential for use in the treatment of a wide variety of clinical conditions ranging from myocardial infarction, to musculoskeletal disorders, and the repair of soft tissue defects. In this work, a custom-designed, 3-dimensional (3-D) scaffold-based perfusion bioreactor system was investigated in the culture of ASCs. Decellularized adipose tissue (DAT) was used to provide a 3-dimensional scaffold, as it possesses the native extracellular matrix (ECM) architecture and composition of human adipose tissue. The DAT had a permeability of 149 m2, based on a perfusion rate of 1.5 mL/min over a 200 mg DAT sample, and the culturing medium was evenly perfused throughout the DAT, thereby permitting possible cell growth within the central regions. Initial culturing studies of human ASCs on tissue culture polystyrene (TCPS) demonstrated that hypoxic (5% O2) conditions decreased the doubling time, and resulted in enhanced cell proliferation, as compared to normoxic (21% O2) conditions. The cell imaging and DNA quantification results showed that suspension seeding of the ASCs permitted cell attachment to the DAT scaffold, but did not support long-term ASC growth. In contrast, when the ASCs were seeded as multicellular aggregates, the cells attached and underwent measurable proliferation. The optimal seeding density observed was 1 x 106 ASCs/scaffold; or 50 aggregates (20,000 ASCs/aggregate) per scaffold. Based on the confocal imaging, the ASCs remained spherical in morphology during the entire culturing period. Moreover, results illustrated that the perfusion bioreactor provided an improved culturing environment for ASCs over traditional static culturing. Hypoxic (5% O2) conditions showed improved proliferation over normoxic (21% O2) conditions, within the bioreactor system. After a 14-day hypoxic culturing period in the perfusion bioreactor, the seeded ASCs retained the ability to undergo adipogenesis, as indicated by Glycerol-3-Phsophate Dehydrogenase (GPDH) enzymatic activity measurements, demonstrating the promise of this approach for soft tissue engineering applications

Subjects/Keywords: Perfusion bioreactor ; Stem cell

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APA (6^th Edition):

Fleming, S. (2011). Development of a Perfusion Bioreactor Strategy for Human Adipose-Derived Stem Cell Expansion . (Thesis). Queens University. Retrieved from http://hdl.handle.net/1974/6867

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Fleming, Sarah. “Development of a Perfusion Bioreactor Strategy for Human Adipose-Derived Stem Cell Expansion .” 2011. Thesis, Queens University. Accessed April 11, 2021. http://hdl.handle.net/1974/6867.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Fleming, Sarah. “Development of a Perfusion Bioreactor Strategy for Human Adipose-Derived Stem Cell Expansion .” 2011. Web. 11 Apr 2021.

Vancouver:

Fleming S. Development of a Perfusion Bioreactor Strategy for Human Adipose-Derived Stem Cell Expansion . [Internet] [Thesis]. Queens University; 2011. [cited 2021 Apr 11]. Available from: http://hdl.handle.net/1974/6867.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Fleming S. Development of a Perfusion Bioreactor Strategy for Human Adipose-Derived Stem Cell Expansion . [Thesis]. Queens University; 2011. Available from: http://hdl.handle.net/1974/6867

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Kansas State University

18. Rand, Taylor Ann. Effect of head up tilt on tumor perfusion in a pre-clinical model of prostate cancer.

Degree: MS, Department of Kinesiology, 2018, Kansas State University

URL: http://hdl.handle.net/2097/39407

► Introduction: Prostate tumor arterioles lack functional smooth muscle and have a diminished myogenic response. Previous research has demonstrated an enhanced prostate tumor blood flow and… (more)

▼ Introduction: Prostate tumor arterioles lack functional smooth muscle and have a diminished myogenic response. Previous research has demonstrated an enhanced prostate tumor blood flow and oxygenation associated with the augmented mean arterial pressure during exercise. Thus, we tested the hypothesis that elevations in the heart-to-prostate tumor hydrostatic gradient via adoption of the 70˚ head-up tilt (HUT) body position would enhance perfusion of the prostate tumor, which may improve tumor oxygenation and radiation therapy outcomes (Study I). Based upon those findings, we performed a secondary analysis (Study II) on previously published prostate hemodynamic responses to an identical tilt-test between young and aged animals. Methods: Study I: Dunning Cell AT-1 tumor cells (100,000) were injected into the ventral lobe of the prostate in male Copenhagen rats (4 mo.; n = 7). Four to six weeks after injection blood flow to the prostate tumor, kidneys, and soleus muscle was measured via the fluorescent microsphere technique in the supine and HUT position. Study II: A secondary analysis was performed on blood flow to the prostate (host tissue of the tumor) in young (6 mo.; n =9) and aged (24 mo.; n=7) male Fisher 344 rats from Ramsey et al., 2007 (39) to determine potential age-associated differences in conductance to this tissue. Results: Study I: No significant difference was observed in blood pressure between the two body positions. Compared to the supine posture, there was a significant reduction in blood flow to the soleus muscle. There was no difference in prostate tumor blood flow or vascular conductance between the supine and HUT position. Study II: In response to tilt, there was a significant reduction in prostate vascular conductance in young rats versus that in the supine posture (P<0.05). In the aged animals, there was no difference in prostate vascular conductance with tilt. Discussion: Contrary to our hypothesis, we did not see any significant differences in either blood flow or vascular conductance to the prostate tumor with manipulations in body position. Importantly, we believe this may be an age-associated effect. Given tumors both co-opt existing arterioles from the host tissue that retain vasomotor control and develop new vessels that lack functional smooth muscle, the enhanced vascular resistance in the prostate with young animals during tilt likely contributed to the lack of change in tumor perfusion with body position given the rats from study I were also young. Given the lack of change in vascular conductance in the prostate with tilt in aged animals, future studies should be performed in aged models of prostate cancer, of which currently there are no immunocompetent aged rodent models of prostate cancer. Advisors/Committee Members: Brad J. Behnke.

Subjects/Keywords: Prostate Cancer Tumor Perfusion

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APA (6^th Edition):

Rand, T. A. (2018). Effect of head up tilt on tumor perfusion in a pre-clinical model of prostate cancer. (Masters Thesis). Kansas State University. Retrieved from http://hdl.handle.net/2097/39407

Chicago Manual of Style (16^th Edition):

Rand, Taylor Ann. “Effect of head up tilt on tumor perfusion in a pre-clinical model of prostate cancer.” 2018. Masters Thesis, Kansas State University. Accessed April 11, 2021. http://hdl.handle.net/2097/39407.

MLA Handbook (7^th Edition):

Rand, Taylor Ann. “Effect of head up tilt on tumor perfusion in a pre-clinical model of prostate cancer.” 2018. Web. 11 Apr 2021.

Vancouver:

Rand TA. Effect of head up tilt on tumor perfusion in a pre-clinical model of prostate cancer. [Internet] [Masters thesis]. Kansas State University; 2018. [cited 2021 Apr 11]. Available from: http://hdl.handle.net/2097/39407.

Council of Science Editors:

Rand TA. Effect of head up tilt on tumor perfusion in a pre-clinical model of prostate cancer. [Masters Thesis]. Kansas State University; 2018. Available from: http://hdl.handle.net/2097/39407

Virginia Tech

19. Freeman, Kendra D. Microangiographic comparison of the effects of the three-loop pulley and six strand Savage tenorrhaphy techniques on the equine superficial digital flexor tendon.

Degree: MS, Biomedical and Veterinary Sciences, 2014, Virginia Tech

URL: http://hdl.handle.net/10919/56839

► Injuries to the equine distal limb are common and often involve synovial, tendinous and/or ligamentous structures. Historically, lacerations involving the equine digital flexor tendons carried… (more)

▼ Injuries to the equine distal limb are common and often involve synovial, tendinous and/or ligamentous structures. Historically, lacerations involving the equine digital flexor tendons carried a poor prognosis for return to athletic function due to contamination of the site at presentation, involvement of multiple anatomic structures and the need for immediate weight bearing after surgery. The need for weight bearing after surgery places strain on the tenorrhaphy site that exceeds the strength of the repair itself. Extrapolation of complex, stronger tenorrhaphy patterns from human literature and applying them to equine patients has been challenging. Human tenorrhaphy techniques initially focused on strong repairs, which are able to match or exceed the strength of tendon itself. Adhesion formation is problematic in human flexor tenorrhaphies, as most injuries occur to tendons surrounded by synovial structures. Human literature now focuses on using repairs that provide initial strength, minimal damage to intrinsic tendon architecture, and allow for early mobilization. This treatment protocol has greatly improved the functional outcome of human tenorrhaphies. Recent studies have evaluated the strength of complex tenorrhaphy patterns in equine superficial digital flexor tendons, using modifications of the Savage technique. The newly evaluated patterns are stronger than previously tested and commonly used techniques, such as the three-loop pulley (3LP). A review of tendon vasculature across species and healing characteristics of tendons highlights the importance of intrinsic tendon vasculature in the healing process. Using tenorrhaphy techniques that preserve this vasculature may improve the clinical outcome in these cases. Only one study has previously evaluated the effect of tenorrhaphy patterns on intrinsic tendon vasculature in equine superficial digital flexor tendon. This study compared perfusion of intrinsic tendon vasculature of equine superficial digital flexor tendon (SDFT) after 3LP and six-strand Savage (SSS) tenorrhaphies. We hypothesized that the SSS technique would significantly decrease vascular perfusion compared to the 3LP technique. Under general anesthesia, eight pairs of forelimb SDFTs were transected and either SSS or 3LP tenorrhaphy was performed on each forelimb. The horses were heparinized, euthanatized, and forelimbs perfused with barium sulfate solution then fixed with formalin under tension. The tendons were transected every 5mm and microangiographic images were obtained using a Faxitron X-ray cabinet with computed radiography imaging. Microvascular analysis of sections proximal to the tenorrhaphy, throughout the tenorrhaphy and distal to the tenorrhaphy was completed using Image J software and a custom macro. A significant reduction in the number of perfused vessels was seen in the SSS compared to the 3LP at two locations within the tenorrhaphy (p=0.004 and 0.039). The SSS technique took on average 4.7 ± 0.9 times longer to place. The SSS technique causes a reduction in tendon… Advisors/Committee Members: Barrett, Jennifer G. (committeechair), White, Nathaniel A. (committee member), Sullins, Kenneth E. (committee member).

Subjects/Keywords: tendon; perfusion; equine; laceration

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APA (6^th Edition):

Freeman, K. D. (2014). Microangiographic comparison of the effects of the three-loop pulley and six strand Savage tenorrhaphy techniques on the equine superficial digital flexor tendon. (Masters Thesis). Virginia Tech. Retrieved from http://hdl.handle.net/10919/56839

Chicago Manual of Style (16^th Edition):

Freeman, Kendra D. “Microangiographic comparison of the effects of the three-loop pulley and six strand Savage tenorrhaphy techniques on the equine superficial digital flexor tendon.” 2014. Masters Thesis, Virginia Tech. Accessed April 11, 2021. http://hdl.handle.net/10919/56839.

MLA Handbook (7^th Edition):

Freeman, Kendra D. “Microangiographic comparison of the effects of the three-loop pulley and six strand Savage tenorrhaphy techniques on the equine superficial digital flexor tendon.” 2014. Web. 11 Apr 2021.

Vancouver:

Freeman KD. Microangiographic comparison of the effects of the three-loop pulley and six strand Savage tenorrhaphy techniques on the equine superficial digital flexor tendon. [Internet] [Masters thesis]. Virginia Tech; 2014. [cited 2021 Apr 11]. Available from: http://hdl.handle.net/10919/56839.

Council of Science Editors:

Freeman KD. Microangiographic comparison of the effects of the three-loop pulley and six strand Savage tenorrhaphy techniques on the equine superficial digital flexor tendon. [Masters Thesis]. Virginia Tech; 2014. Available from: http://hdl.handle.net/10919/56839

University of Sydney

20. Hameed, Ahmer Mohammad. Modifying Donor Organ Retrieval and Preservation to Enhance Transplant Outcomes .

Degree: 2018, University of Sydney

URL: http://hdl.handle.net/2123/20363

► The last 1-2 decades have seen remarkable advances in organ procurement and preservation practices, especially with renewed enthusiasm for machine perfusion (MP) technology. However, cold… (more)

▼ The last 1-2 decades have seen remarkable advances in organ procurement and preservation practices, especially with renewed enthusiasm for machine perfusion (MP) technology. However, cold static storage (CS) remains the most popular world-wide approach for the preservation of organs such as the kidneys, liver, and pancreas, largely due to its simplicity. It is clear that CS techniques have limited potential for further improvement, and will likely be supplanted and/or supplemented with MP technologies over the coming years due to the reparative, resuscitative, and assessment capabilities afforded by MP. This is especially important as we increase our utilisation of marginal and/or donation after circulatory death (DCD) organs to meet the ever-increasing demand requirements for transplantation. This dissertation explores selected aspects of abdominal organ procurement and preservation as targets for improvement and/or modification with the aim to enhance recipient transplantation outcomes. The kidney is used as a model organ for the development and exploration of MP as a means to ameliorate transplant organ ischaemia-reperfusion injury (IRI), including through the targeted delivery of anti-IRI drugs. In contrast, the optimization of CS protocols, including identification of ideal perfusion fluids and in situ perfusion routes, forms the basis for liver and pancreas transplantation work in this thesis. Such investigations are necessary to promote uniformity of practice between centres, and allow appropriate comparisons between MP and CS. The kidney MP work was guided by a systematic review and meta-analysis comparing MP and CS in the clinical and pre-clinical setting. Although hypothermic MP (HMP) was shown to enhance short-term graft outcomes, results were equivocal with respect to graft survival, especially in the DCD setting. Preliminary evidence indicated the potential superiority of normothermic MP (NMP) above HMP or CS, which may be further enhanced by using NMP as a conduit for directed drug delivery to the kidney to ameliorate IRI. We therefore developed and optimized a local NMP set-up using a series of porcine kidneys, which was then utilized to deliver the anti-IRI agent CD47-blocking antibody (αCD47Ab) in a porcine DCD model. The significant potential of this agent was initially confirmed by testing in a murine model of severe warm IRI, including its comparative efficacy to two other promising IRI agents, soluble complement receptor 1 (sCR1), and recombinant thrombomodulin, and also sCR1 in combination with αCD47Ab. αCD47Ab was successfully delivered to porcine DCD kidneys using NMP, with subsequent downstream positive impacts upon renal perfusion, and some functional and IRI-related parameters. The clinical utilisation of renal NMP has so far been limited to the UK, and this modality has not been tested in human kidneys in Australasia. Furthermore, the mechanistic basis of brief renal NMP is not entirely clear. Therefore, and as a prelude to a phase I clinical trial, NMP was tested in discarded deceased…

Subjects/Keywords: Transplantation; organ preservation; machine perfusion

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Hameed, A. M. (2018). Modifying Donor Organ Retrieval and Preservation to Enhance Transplant Outcomes . (Thesis). University of Sydney. Retrieved from http://hdl.handle.net/2123/20363

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Hameed, Ahmer Mohammad. “Modifying Donor Organ Retrieval and Preservation to Enhance Transplant Outcomes .” 2018. Thesis, University of Sydney. Accessed April 11, 2021. http://hdl.handle.net/2123/20363.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Hameed, Ahmer Mohammad. “Modifying Donor Organ Retrieval and Preservation to Enhance Transplant Outcomes .” 2018. Web. 11 Apr 2021.

Vancouver:

Hameed AM. Modifying Donor Organ Retrieval and Preservation to Enhance Transplant Outcomes . [Internet] [Thesis]. University of Sydney; 2018. [cited 2021 Apr 11]. Available from: http://hdl.handle.net/2123/20363.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Hameed AM. Modifying Donor Organ Retrieval and Preservation to Enhance Transplant Outcomes . [Thesis]. University of Sydney; 2018. Available from: http://hdl.handle.net/2123/20363

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

21. JoÃo Alison de Moraes Silveira. Efeitos renais e alteraÃÃes morfolÃgicas causadas pelo peptÃdeo natriurÃtico sintÃtico do veneno Crotalus durissus cascavella.

Degree: 2015, Universidade Federal do CearÃ; Programa de PÃs-GraduaÃÃo em Farmacologia; UFC; BR

URL: http://www.teses.ufc.br/tde_busca/arquivo.php?codArquivo=13878

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Conselho Nacional de Desenvolvimento CientÃfico e TecnolÃgico

Os PeptÃdeos NatriurÃticos (NPs) tÃm significativa participaÃÃo na regulaÃÃo na homeostasia cardiovascular, renal e endÃcrina, e tÃm sido… (more)

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Conselho Nacional de Desenvolvimento CientÃfico e TecnolÃgico

Os PeptÃdeos NatriurÃticos (NPs) tÃm significativa participaÃÃo na regulaÃÃo na homeostasia cardiovascular, renal e endÃcrina, e tÃm sido descritos nos venenos de serpentes peÃonhentas. A Crotalus durissus cascavella, uma serpente terrÃcola caracterÃstica da caatinga do Nordeste do Brasil, possui em seu veneno total um NP (NPCdc), ao qual sÃo relatados efeitos vasculares e renais. O objetivo desse trabalho foi sintetizar em fase sÃlida o NPCdc e avaliar suas aÃÃes renais atravÃs da perfusÃo de rim isolado e em cultura de cÃlulas tubulares renais da linhagem MDCK e LLC-MK2. Rins de ratos Wistar machos, pesando entre 250-300 g, foram excisados cirurgicamente e perfundidos com soluÃÃo de Krebs-Henseleit modificada com 6% p/v de albumina bovina previamente dialisada. Foram investigados os efeitos do NPCdc em quatro concentraÃÃes (0,03 Âg/mL; 0,1 Âg/mL; 0,3 Âg/mL e 1 Âg/mL; n=6). As cÃlulas MDCK e LLC-MK2 foram cultivadas em meio de cultura RPMI 1640 suplementado com 10% v/v de Soro Bovino Fetal e entÃo avaliadas na presenÃa do NPCdc em diversas concentraÃÃes em um perÃodo de incubaÃÃo de 24 horas. ApÃs esse perÃodo, foram realizados ensaios de viabilidade celular. Os dados foram comparados estatisticamente considerando P<0,05. Houve aumento na pressÃo de perfusÃo (PP) em 0,03 Âg/mL e reduÃÃo em 1 Âg/mL. A resistÃncia vascular renal (RVR) apresentou aumento em 0,03 Âg/mL. O fluxo urinÃrio (FU) aumentou em 0,03 Âg/mL e diminuiu em 0,1 Âg/mL e 1 Âg/mL. O ritmo de filtraÃÃo glomerular (RFG) encontrou-se diminuÃdo nas quatro concentraÃÃes testadas. O clearance osmolar (COsm) apresentou-se aumentado em 0,03 μg/mL e reduzido em 0,1 μg/mL e 1 μg/mL. O percentual de transporte tubular total e proximal de sÃdio (%TNa+ e %TpNa+, respectivamente) e cloreto (%TCl- e %TpCl-, respectivamente) apresentaram reduÃÃes nas quatro concentraÃÃes testadas. JÃ o percentual de transporte tubular total e proximal de potÃssio (%TK+ e %TpK+, respectivamente) apresentaram-se reduzidos em 0,03 μg/mL e 0,3 μg/mL. A anÃlise histopatolÃgica mostrou a presenÃa de alteraÃÃes morfolÃgicas significativas, como degeneraÃÃo hidrÃpica concentraÃÃo-dependente em todas as concentraÃÃes, juntamente com discreta a moderada deposiÃÃo de material proteÃceo nos tÃbulos na concentraÃÃo de 0,03 μg/mL. Na cultura de cÃlulas MDCK e LLC-MK2, porÃm, o NPCdc nÃo foi capaz de diminuir a viabilidade celular. Esses resultados demonstram que o NPCdc modificou todos os parÃmetros avaliados na perfusÃo de rim isolado, alÃm de revelar alteraÃÃes de carÃter citotÃxico na anÃlise histopatolÃgica dos mesmos, todavia, nÃo as apresentando em culturas de cÃlulas tubulares renais.

Natriuretic Peptides (NPs) have significant interest in regulating the cardiovascular, renal and endocrine homeostasis, and have been described in the venom of venomous snakes. Crotalus durissus cascavella, a characteristic terrestrial snake of caatinga biome of northeastern Brazil, has in his whole venom an NP…

Advisors/Committee Members: Helena Serra Azul Monteiro, Gandhi RÃdis Baptista, Nilberto Robson FalcÃo do Nascimento.

Subjects/Keywords: Crotalus; Perfusion; Cell Survival; FARMACOLOGIA

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Silveira, J. A. d. M. (2015). Efeitos renais e alteraÃÃes morfolÃgicas causadas pelo peptÃdeo natriurÃtico sintÃtico do veneno Crotalus durissus cascavella. (Masters Thesis). Universidade Federal do CearÃ; Programa de PÃs-GraduaÃÃo em Farmacologia; UFC; BR. Retrieved from http://www.teses.ufc.br/tde_busca/arquivo.php?codArquivo=13878

Chicago Manual of Style (16^th Edition):

Silveira, JoÃo Alison de Moraes. “Efeitos renais e alteraÃÃes morfolÃgicas causadas pelo peptÃdeo natriurÃtico sintÃtico do veneno Crotalus durissus cascavella.” 2015. Masters Thesis, Universidade Federal do CearÃ; Programa de PÃs-GraduaÃÃo em Farmacologia; UFC; BR. Accessed April 11, 2021. http://www.teses.ufc.br/tde_busca/arquivo.php?codArquivo=13878.

MLA Handbook (7^th Edition):

Silveira, JoÃo Alison de Moraes. “Efeitos renais e alteraÃÃes morfolÃgicas causadas pelo peptÃdeo natriurÃtico sintÃtico do veneno Crotalus durissus cascavella.” 2015. Web. 11 Apr 2021.

Vancouver:

Silveira JAdM. Efeitos renais e alteraÃÃes morfolÃgicas causadas pelo peptÃdeo natriurÃtico sintÃtico do veneno Crotalus durissus cascavella. [Internet] [Masters thesis]. Universidade Federal do CearÃ; Programa de PÃs-GraduaÃÃo em Farmacologia; UFC; BR; 2015. [cited 2021 Apr 11]. Available from: http://www.teses.ufc.br/tde_busca/arquivo.php?codArquivo=13878.

Council of Science Editors:

Silveira JAdM. Efeitos renais e alteraÃÃes morfolÃgicas causadas pelo peptÃdeo natriurÃtico sintÃtico do veneno Crotalus durissus cascavella. [Masters Thesis]. Universidade Federal do CearÃ; Programa de PÃs-GraduaÃÃo em Farmacologia; UFC; BR; 2015. Available from: http://www.teses.ufc.br/tde_busca/arquivo.php?codArquivo=13878

McGill University

22. Lalonde, Donald H. Bone Grafting: A Comparative Analysis of Vascularized and Non Vascularized Autografts.

Degree: MS, Department of Plastic Surgery, 1982, McGill University

URL: https://escholarship.mcgill.ca/downloads/7p88ck02h.pdf ; https://escholarship.mcgill.ca/concern/theses/bc386m86v

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Vascular perfusion studies, histology, tetracycline labelling, and technitium99 scintigraphy were used to compare conventional rib grafts with and without periosteum to rib grafts vascularized by… (more)

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Vascular perfusion studies, histology, tetracycline labelling, and technitium99 scintigraphy were used to compare conventional rib grafts with and without periosteum to rib grafts vascularized by vascular bundle implantation, by dermis island flaps, and by microvascular anastomosis of graft nutrient vessels in dogs. Results indicated that: 1) The vascular pattern of posterior microvascularized rib grafts is identical to that of normal ribs. 2) Implanted vascular bundles remain patent and revascularize bone grafts. 3) Circulation in rib grafts and the patency of anastomoses of microvascularized rib grafts are accurately reflected in technitium99 bone scans. 4) Tetracycline labelling is not a reliable indicator of the patency of a bone graft's pedicle blood supply. 5) The vascular invasion of failed microvascularlzed rib grafts is slower than that of conventional rib grafts. 6) The presence or absence of periosteum has no observable effect on graft revascularization. 7) Dermis island flaps did not enhance graft vascularization.

Nous avons mené des études de perfusion vasculaire, de marquage à la tétracycline, de scintigraphie osseuse au technitium99 et d'histologie pour comparer les greffes costales conventionelles, avec ou sans périoste, aux greffes costales nourries par implantation du paquet vasculaire, par ilot dermique et par anastomose microvasculaire des vaisseaux nourriciers du greffon dans le chien. Nos résultats indiquent que: 1) La vasculature des greffons costaux postérieurs microvascularisés est identique à celle des cotes normales. 2)Les implants du paquet vasculaire demeurent perméables et revascularisent les greffes osseuses. 3) la scintigraphie au technitium99 est un reflet précis de la circulation dans les greffons osseux et de la perméabilité des anastomoses dans les greffons microvascularises, 4)Le marquage a la tétracycline n'est pas un indicateur fiable de la perméabilité des vasseaux sanguins nourriciers de greffons osseux. 5)L'invasion vasculaire d'un greffon costal microvascularisé non-perméable est plus lente· que celle observée dans un greffon costal conventional. 6)La présence ou l'absence de périoste n'a pas d'effet observable sur la revascularisation du greffon, 7)Les ilots dermiques n’ont pas accéléré la vascularisation des greffons.

Advisors/Committee Members: Williams, Bruce.

Subjects/Keywords: Vascular perfusion

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Lalonde, D. H. (1982). Bone Grafting: A Comparative Analysis of Vascularized and Non Vascularized Autografts. (Masters Thesis). McGill University. Retrieved from https://escholarship.mcgill.ca/downloads/7p88ck02h.pdf ; https://escholarship.mcgill.ca/concern/theses/bc386m86v

Chicago Manual of Style (16^th Edition):

Lalonde, Donald H. “Bone Grafting: A Comparative Analysis of Vascularized and Non Vascularized Autografts.” 1982. Masters Thesis, McGill University. Accessed April 11, 2021. https://escholarship.mcgill.ca/downloads/7p88ck02h.pdf ; https://escholarship.mcgill.ca/concern/theses/bc386m86v.

MLA Handbook (7^th Edition):

Lalonde, Donald H. “Bone Grafting: A Comparative Analysis of Vascularized and Non Vascularized Autografts.” 1982. Web. 11 Apr 2021.

Vancouver:

Lalonde DH. Bone Grafting: A Comparative Analysis of Vascularized and Non Vascularized Autografts. [Internet] [Masters thesis]. McGill University; 1982. [cited 2021 Apr 11]. Available from: https://escholarship.mcgill.ca/downloads/7p88ck02h.pdf ; https://escholarship.mcgill.ca/concern/theses/bc386m86v.

Council of Science Editors:

Lalonde DH. Bone Grafting: A Comparative Analysis of Vascularized and Non Vascularized Autografts. [Masters Thesis]. McGill University; 1982. Available from: https://escholarship.mcgill.ca/downloads/7p88ck02h.pdf ; https://escholarship.mcgill.ca/concern/theses/bc386m86v

Universiteit Utrecht

23. Grootenboers, M.J.J.H. Regional Chemotherapy of the Lung: Investigations of Isolated Lung Perfusion and Selective Pulmonary Artery Perfusion.

Degree: 2008, Universiteit Utrecht

URL: http://dspace.library.uu.nl:8080/handle/1874/25766

► The aim of this thesis was two-fold. First, - part I of the dissertation -, to explore ILuP with melphalan and hyperthermia followed by pulmonary… (more)

Subjects/Keywords: Geneeskunde; isolated lung perfusion; pulmonary artery perfusion; melphalan; gemcitabine

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Grootenboers, M. J. J. H. (2008). Regional Chemotherapy of the Lung: Investigations of Isolated Lung Perfusion and Selective Pulmonary Artery Perfusion. (Doctoral Dissertation). Universiteit Utrecht. Retrieved from http://dspace.library.uu.nl:8080/handle/1874/25766

Chicago Manual of Style (16^th Edition):

Grootenboers, M J J H. “Regional Chemotherapy of the Lung: Investigations of Isolated Lung Perfusion and Selective Pulmonary Artery Perfusion.” 2008. Doctoral Dissertation, Universiteit Utrecht. Accessed April 11, 2021. http://dspace.library.uu.nl:8080/handle/1874/25766.

MLA Handbook (7^th Edition):

Grootenboers, M J J H. “Regional Chemotherapy of the Lung: Investigations of Isolated Lung Perfusion and Selective Pulmonary Artery Perfusion.” 2008. Web. 11 Apr 2021.

Vancouver:

Grootenboers MJJH. Regional Chemotherapy of the Lung: Investigations of Isolated Lung Perfusion and Selective Pulmonary Artery Perfusion. [Internet] [Doctoral dissertation]. Universiteit Utrecht; 2008. [cited 2021 Apr 11]. Available from: http://dspace.library.uu.nl:8080/handle/1874/25766.

Council of Science Editors:

Grootenboers MJJH. Regional Chemotherapy of the Lung: Investigations of Isolated Lung Perfusion and Selective Pulmonary Artery Perfusion. [Doctoral Dissertation]. Universiteit Utrecht; 2008. Available from: http://dspace.library.uu.nl:8080/handle/1874/25766

24. Romain, Blandine. Modélisation de la perfusion abdominale sur des séquences dynamiques d'images tomodensitométriques avec injection de produit de constraste : Modeling of abdominal perfusion on CT image sequences with contrast product injection.

Degree: Docteur es, Mathématiques appliquées, 2014, Châtenay-Malabry, Ecole centrale de Paris

URL: http://www.theses.fr/2014ECAP0005

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L'objectif général du travail de cette thèse est de proposer des méthodes robustes pour permettre d’obtenir des critères sur l’évolution de la pathologie tumorale à… (more)

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L'objectif général du travail de cette thèse est de proposer des méthodes robustes pour permettre d’obtenir des critères sur l’évolution de la pathologie tumorale à partir d’études dynamiques. Actuellement, l’appréciation de l’efficacité d’un traitement antiangiogénique (destruction des vaisseaux alimentant la tumeur) repose principalement sur l’imagerie fonctionnelle dont l’objectif est de quantifier la microcirculation tumorale à partir d’acquisitions dynamiques de perfusion. Cependant, différentes limites concernant le suivi de la réponse précoce des lésions par imagerie existent (mauvaise maîtrise des mouvements respiratoires, pas de consensus sur les paramètres permettant de quantifier la microcirculation tumorale, estimation paramétrique faite à partir de données extrêmement bruitées et pour un grand nombre de zones - une estimation par voxel de la séquence dynamique d’images). Dans un contexte clinique extrêmement contraignant, nous avons mis en place un cadre rigoureux comprenant l’ensemble des étapes nécessaires pour une caractérisation plus fiable de la microcirculation tumorale à partir de séquences d’images acquises sous perfusion de produit de contraste : les contributions principales de cette thèse couvrent ainsi l’optimisation des paramètres de reconstruction, le développement d’une méthode de recalage adaptée à nos données, la sélection argumentée d’un modèle de perfusion et enfin le développement d’une méthode robuste d’estimation des paramètres. Ces travaux permettent d’envisager l’utilisation des modèles de perfusion pour la caractérisation et la prédiction de la réponse d’un patient à différents traitements antitumoraux.

The main objective is to propose robust methods to allow estimation of functional markers reflecting the tumor evolution from dynamic studies. Currently, in this domain, assessing of the efficiency evaluation of an anti-angiogenic therapy (destruction of vessels which feed the tumor) is mainly based on the functional imaging of the microcirculation, which the objective is to quantify the tumor microcirculation by dynamic acquisitions with injection of contrast product. However, several limitations are present (lack of control of the breathing movement, no consensus on the parameters permitting the quantification of tumor microcirculation, parameter estimation computed from noisy data and a large number of regions - one estimation by voxel or group of voxel of the dynamic image sequence). In a restrictive clinical context (noisy data, few number), we have developed a complete pipeline with a set of necessary steps to a reliable characterization of the tumor microcirculation from dynamic perfusion image sequence: the main contributions of this thesis cover the reconstruction parameters optimization, the development of a registration method, the argued selection of a perfusion model and the development of a robust method of parameter estimation. With these works, we can envision the utilization of these perfusion models to the characterization and the prediction of the therapy…

Advisors/Committee Members: Alché-Buc, Florence d' (thesis director).

Subjects/Keywords: TDM de perfusion; Recalage; Estimation paramétrique; Perfusion CT; Registration; Parameter estimation

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Romain, B. (2014). Modélisation de la perfusion abdominale sur des séquences dynamiques d'images tomodensitométriques avec injection de produit de constraste : Modeling of abdominal perfusion on CT image sequences with contrast product injection. (Doctoral Dissertation). Châtenay-Malabry, Ecole centrale de Paris. Retrieved from http://www.theses.fr/2014ECAP0005

Chicago Manual of Style (16^th Edition):

Romain, Blandine. “Modélisation de la perfusion abdominale sur des séquences dynamiques d'images tomodensitométriques avec injection de produit de constraste : Modeling of abdominal perfusion on CT image sequences with contrast product injection.” 2014. Doctoral Dissertation, Châtenay-Malabry, Ecole centrale de Paris. Accessed April 11, 2021. http://www.theses.fr/2014ECAP0005.

MLA Handbook (7^th Edition):

Romain, Blandine. “Modélisation de la perfusion abdominale sur des séquences dynamiques d'images tomodensitométriques avec injection de produit de constraste : Modeling of abdominal perfusion on CT image sequences with contrast product injection.” 2014. Web. 11 Apr 2021.

Vancouver:

Romain B. Modélisation de la perfusion abdominale sur des séquences dynamiques d'images tomodensitométriques avec injection de produit de constraste : Modeling of abdominal perfusion on CT image sequences with contrast product injection. [Internet] [Doctoral dissertation]. Châtenay-Malabry, Ecole centrale de Paris; 2014. [cited 2021 Apr 11]. Available from: http://www.theses.fr/2014ECAP0005.

Council of Science Editors:

Romain B. Modélisation de la perfusion abdominale sur des séquences dynamiques d'images tomodensitométriques avec injection de produit de constraste : Modeling of abdominal perfusion on CT image sequences with contrast product injection. [Doctoral Dissertation]. Châtenay-Malabry, Ecole centrale de Paris; 2014. Available from: http://www.theses.fr/2014ECAP0005

Université de Grenoble

25. Debacker, Clément. Développement de l'imagerie de perfusion cérébrale par marquage des spins artériels : Development of brain perfusion imaging using arterial spin labeling.

Degree: Docteur es, Physique pour les Sciences du Vivant, 2014, Université de Grenoble

URL: http://www.theses.fr/2014GRENY018

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Ce travail de thèse, principalement méthodologique, s'est intéressé aux techniques d'imagerie par résonance magnétique (IRM) permettant de mesurer le flux sanguin cérébral (CBF) et plus… (more)

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Ce travail de thèse, principalement méthodologique, s'est intéressé aux techniques d'imagerie par résonance magnétique (IRM) permettant de mesurer le flux sanguin cérébral (CBF) et plus particulièrement aux techniques de marquage de spins artériels (ASL), qui utilisent les protons de l'eau du sang comme marqueur. Nous avons mis en place la séquence ASL de marquage pseudo-continu (pCASL) et évalué sa réponse à un stimulus hypercapnique. Nous avons évalué différentes stratégies pour optimiser l'efficacité d'inversion. Pour cela, nous avons également mis en place des outils de simulations numériques des approches ASL. Nos résultats démontrent que l'efficacité d'inversion est influencée par l'homogénéité du champ magnétique dans la région de marquage, ce qui pose un problème à haut champ magnétique. Le protocole pCASL optimisé a ensuite été évalué chez le rat à trois champ magnétiques (4.7, 7, et 11.7T) et comparé avec une approche en ASL continu classique (CASL). Cette comparaison a montré une excellente reproductibilité inter-animal et inter-champ de la méthode développée. Dans une seconde partie, nous nous sommes également intéressés à l'influence du temps de relaxation longitudinal (T1) du tissu cérébral sur les valeurs du CBF calculées. Pour cela, nous avons modifié le T1 du tissu par une injection intra-cérébrale de manganèse. Cette étude a montré la difficulté de prendre en compte le changement de T1 du tissu. Dans une troisième partie, nous avons évalué l'apport d'une antenne de marquage spécifique pour l'approche CASL en comparant les mesures de CBF obtenues avec celles de la pCASL. Nous avons observé une bonne concordance entre ces deux méthodes à travers les coupes. Nos résultats illustrent également l'importante contribution du transfert d'aimantation dans les séquences de CASL. Les outils développés au cours de cette thèse sont en cours d'application dans des protocoles d'étude de modèles de tumeurs cérébrales, d'accident vasculaire cérébral et de traumatisme crânien.

This PhD work, mainly methodological, focused on the techniques of magnetic resonance imaging (MRI) to measure cerebral blood flow (CBF) and more particularly on arterial spin labeling (ASL), which uses water protons from the blood as markers. We implemented the pseudo-continuous ASL labeling sequence (pCASL) and evaluated its response to a hypercapnic stimulus. We evaluated different strategies to maximize the labeling inversion effeciency. For this, we implemented numerical simulation tools of ASL approaches. Our results demonstrated that the inversion efficiency is influenced by the homogeneity of the magnetic field in the labeling region, which becomes a problem at high magnetic field. The optimized pCASL protocol was then evaluated in rats at three magnetic fields (4.7, 7, and 11.7T) and compared with a conventional continuous ASL approach (CASL). This comparison showed excellent inter-animal and inter-field reproducibility of the developed method. In a second part, we evaluated the influence of the longitudinal relaxation time (T1) of…

Advisors/Committee Members: Barbier, Emmanuel (thesis director), Warnking, Jan (thesis director).

Subjects/Keywords: IRM; Cerveau; Perfusion; Asl; MRI; Brain; Perfusion; Asl; 570

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Debacker, C. (2014). Développement de l'imagerie de perfusion cérébrale par marquage des spins artériels : Development of brain perfusion imaging using arterial spin labeling. (Doctoral Dissertation). Université de Grenoble. Retrieved from http://www.theses.fr/2014GRENY018

Chicago Manual of Style (16^th Edition):

Debacker, Clément. “Développement de l'imagerie de perfusion cérébrale par marquage des spins artériels : Development of brain perfusion imaging using arterial spin labeling.” 2014. Doctoral Dissertation, Université de Grenoble. Accessed April 11, 2021. http://www.theses.fr/2014GRENY018.

MLA Handbook (7^th Edition):

Debacker, Clément. “Développement de l'imagerie de perfusion cérébrale par marquage des spins artériels : Development of brain perfusion imaging using arterial spin labeling.” 2014. Web. 11 Apr 2021.

Vancouver:

Debacker C. Développement de l'imagerie de perfusion cérébrale par marquage des spins artériels : Development of brain perfusion imaging using arterial spin labeling. [Internet] [Doctoral dissertation]. Université de Grenoble; 2014. [cited 2021 Apr 11]. Available from: http://www.theses.fr/2014GRENY018.

Council of Science Editors:

Debacker C. Développement de l'imagerie de perfusion cérébrale par marquage des spins artériels : Development of brain perfusion imaging using arterial spin labeling. [Doctoral Dissertation]. Université de Grenoble; 2014. Available from: http://www.theses.fr/2014GRENY018

Université de Grenoble

26. Bouvier, Julien. Méthodologie et évaluation clinique de l'imagerie de l'oxygénation cérébrale par IRM : Methodology and clinical evaluation of cerebral oxygenation using MRI.

Degree: Docteur es, Biotechnologie, instrumentation, signal et imagerie pour la biologie, la médecine et l'environnement, 2013, Université de Grenoble

URL: http://www.theses.fr/2013GRENS036

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Ce travail méthodologique a porté sur la mise en place et l'évaluation d'une technique de cartographie de l'oxygénation cérébrale en imagerie par résonance magnétique (IRM)… (more)

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Ce travail méthodologique a porté sur la mise en place et l'évaluation d'une technique de cartographie de l'oxygénation cérébrale en imagerie par résonance magnétique (IRM) à 3 T. La technique repose sur la quantification de signaux IRM issus du contraste BOLD (pour « blood level oxygen dependent ») à l'aide d'une approche multiparamétrique (approche mqBOLD pour multiparametric quantitative BOLD) et permet d'obtenir une estimation de la saturation tissulaire en oxygène (StO2). Un premier travail de simulation numérique a porté sur la prise en compte du biais induit par la susceptibilité magnétique de la myéline dans la mesure de StO2. Ce travail a permis de montrer l'influence de l'orientation des fibres de substance blanche par rapport au champ magnétique principal sur la mesure de StO2. La méthode mqBOLD a tout d'abord été appliquée chez l'animal, sur le primate non-humain. L'approche semble suffisamment précise pour observer des différences inter-espèces. Puis, cette approche a été adaptée pour une utilisation en clinique et appliquée à différentes pathologies. Chez des patients souffrant d'un accident vasculaire cérébral à la phase aiguë, nous avons observé une différence spatiale entre les régions pour lesquelles le débit sanguin et la StO2 sont abaissés, différence qui répondrait aux critères définissant la pénombre ischémique. Chez des patients atteints d'une sténose intracrânienne artérielle sévère, nous avons étudié la relation entre la mesure de la vasoréactivité cérébrale avec celle de l'oxygénation. Les résultats montrent une corrélation entre l'altération de la vasoréactivité et celle de l'oxygénation dans le territoire de l'artère cérébrale moyenne. Enfin, nous montrons au travers d'une analyse par cluster que l'étude de l'oxygénation apporte des informations complémentaires aux paramètres de perfusion dans la détermination du grade tumoral. L'ensemble des résultats obtenus au cours de ce travail démontrent la faisabilité de la cartographie de StO2 par IRM et la complémentarité de cette approche avec les techniques de caractérisation de perfusion tissulaire.

This work focuses on the implementation and the evaluation of a technique to map cerebral oxygenation by MRI at 3T. The technique is based on the quantification of MRI signals from the BOLD contrast (for "blood oxygen level dependent") using a multiparametric (mqBOLD multiparametric approach for quantitative BOLD) and provides an estimate of the tissue oxygen saturation (StO2). A study based on a numerical simulation focused on taking into account the bias induced by the magnetic susceptibility of myelin in the measurement of StO2. This work demonstrated the influence of the orientation of white matter fibers with respect to the main magnetic field on the measurement of StO2. The mqBOLD method was applied in animals, the non-human primate. The approach seemed accurate enough to observe differences between species. Then, this approach was adapted for clinical practice and applied to different pathologies. In acute stroke patients, we observed a…

Advisors/Committee Members: Krainik, Alexandre (thesis director), Barbier, Emmanuel (thesis director).

Subjects/Keywords: IRM; Oxygénation; Cerveau; Perfusion; Vasoréactivité; MRI; Oxygenation; Brain; Perfusion; Vasoreactivity; 610

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Bouvier, J. (2013). Méthodologie et évaluation clinique de l'imagerie de l'oxygénation cérébrale par IRM : Methodology and clinical evaluation of cerebral oxygenation using MRI. (Doctoral Dissertation). Université de Grenoble. Retrieved from http://www.theses.fr/2013GRENS036

Chicago Manual of Style (16^th Edition):

Bouvier, Julien. “Méthodologie et évaluation clinique de l'imagerie de l'oxygénation cérébrale par IRM : Methodology and clinical evaluation of cerebral oxygenation using MRI.” 2013. Doctoral Dissertation, Université de Grenoble. Accessed April 11, 2021. http://www.theses.fr/2013GRENS036.

MLA Handbook (7^th Edition):

Bouvier, Julien. “Méthodologie et évaluation clinique de l'imagerie de l'oxygénation cérébrale par IRM : Methodology and clinical evaluation of cerebral oxygenation using MRI.” 2013. Web. 11 Apr 2021.

Vancouver:

Bouvier J. Méthodologie et évaluation clinique de l'imagerie de l'oxygénation cérébrale par IRM : Methodology and clinical evaluation of cerebral oxygenation using MRI. [Internet] [Doctoral dissertation]. Université de Grenoble; 2013. [cited 2021 Apr 11]. Available from: http://www.theses.fr/2013GRENS036.

Council of Science Editors:

Bouvier J. Méthodologie et évaluation clinique de l'imagerie de l'oxygénation cérébrale par IRM : Methodology and clinical evaluation of cerebral oxygenation using MRI. [Doctoral Dissertation]. Université de Grenoble; 2013. Available from: http://www.theses.fr/2013GRENS036

27. Minnella, Walter Settimo Leonardo. Development of microfluidic tools for biological applications : Développement d'outils microfluidiques pour des applications biologiques.

Degree: Docteur es, Physico-chimie de la matière condensée, 2017, Bordeaux

URL: http://www.theses.fr/2017BORD0664

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Cette thèse traite le développement de dispositifs, basés sur la technologie "laboratoire sur puce"(LOC) qui visent à contrôler l'environnement des systèmes biologiques pour des applications… (more)

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Cette thèse traite le développement de dispositifs, basés sur la technologie "laboratoire sur puce"(LOC) qui visent à contrôler l'environnement des systèmes biologiques pour des applications macro et microbiologiques. En effet, les caractéristiques de la microfluidique permettent de manipuler l'environnement cellulaire à un niveau supérieur à celui du degré de contrôle atteignable avec les techniques ordinaires. Dans ce travail de thèse sera explorée la possibilité de profiter de ces fonctions afin de développer des outils de diagnostic peu coûteux et pourtant efficaces. En particulier, on rapporte le développement de systèmes microfluidiques permettant une perfusion des médias fluide et rapide, ainsi qu'une plateforme LOC capable de réaliser des PCRq hautement multiplexes. Au sujet des systèmes de perfusion, le but était d'obtenir une substitution du médium entourant les particules afin d'augmenter les capacités de séparation des modules de tri microfluidiques couplés. L'efficacité de notre approche a été validée par les hauts taux de séparation obtenus (>90%) avec l'utilisation de notre système de perfusion microfluidique couplé à une puce d'acoustophorèse. De plus, nous avons conçu et développé un système de thermalisation microfluidique capable d'opérer des changements de température en moins de 1s. Plus spécifiquement, cette plateforme exploite l'échange de chaleur entre un liquide de thermalisation qui circule dans une puce microfluidique et l'échantillon. Ces performances de thermalisation, et le rapport surface/volume élevé typique des appareils microfluidiques, ont permis d'effectuer 50 cycles de PCRq et l'analyse de courbe de fusion en moins de dix minutes.

The topic of this manuscript is the development of microdevices, based on "lab on chip" (LOC) technology, aimed to the environmental control and regulation of biological systems for macro and microbiological applications. Indeed, microfluidics possesses some inherent features which allow the manipulation of the environment at the cell and sub-cell level which are superior than the degree of control achievable with standard techniques. In this thesis work the possibility to leverage these features to develop inexpensive yet effective diagnostic tools is explored. In particular, we report the development of microfluidic systems which allow seamless and fast media perfusion and a novel LOC platform capable of performing highly multiplexed real-time PCR assays. Concerning the microfluidic perfusion systems, the aim was to achieve in-flow substitution of the particles' surrounding media in order to enhance the separation capabilities of the coupled microfluidic sorting modules. The effectiveness of our approach was validated by obtaining high separation purities (>90%) using our microfluidic perfusion system coupled with an acoustophoresis chip to discern two population of micro-sized beads. Moreover, we conceived and developed a microfluidic thermalisation system capable of sub-second temperature switches. Specifically, this platform relies on conductive…

Advisors/Committee Members: Sandre, Olivier (thesis director).

Subjects/Keywords: Microfluidique; PCRq; Perfusion; Biotechnologie; Microfluidics; QPCR; Perfusion; Biotechnology

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Minnella, W. S. L. (2017). Development of microfluidic tools for biological applications : Développement d'outils microfluidiques pour des applications biologiques. (Doctoral Dissertation). Bordeaux. Retrieved from http://www.theses.fr/2017BORD0664

Chicago Manual of Style (16^th Edition):

Minnella, Walter Settimo Leonardo. “Development of microfluidic tools for biological applications : Développement d'outils microfluidiques pour des applications biologiques.” 2017. Doctoral Dissertation, Bordeaux. Accessed April 11, 2021. http://www.theses.fr/2017BORD0664.

MLA Handbook (7^th Edition):

Minnella, Walter Settimo Leonardo. “Development of microfluidic tools for biological applications : Développement d'outils microfluidiques pour des applications biologiques.” 2017. Web. 11 Apr 2021.

Vancouver:

Minnella WSL. Development of microfluidic tools for biological applications : Développement d'outils microfluidiques pour des applications biologiques. [Internet] [Doctoral dissertation]. Bordeaux; 2017. [cited 2021 Apr 11]. Available from: http://www.theses.fr/2017BORD0664.

Council of Science Editors:

Minnella WSL. Development of microfluidic tools for biological applications : Développement d'outils microfluidiques pour des applications biologiques. [Doctoral Dissertation]. Bordeaux; 2017. Available from: http://www.theses.fr/2017BORD0664

Delft University of Technology

28. Amesz, Jorik (author). Cardiac mapping on ex vivo perfused porcine slaughterhouse hearts: A Langendorff perfusion protocol for epicardial mapping on isolated beating hearts.

Degree: 2020, Delft University of Technology

URL: http://resolver.tudelft.nl/uuid:e2bc6f6b-ed3f-44b0-b851-74ea066a47c6

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Cardiac mapping techniques, in which an electrode-array is placed directly on the surface of the heart, were developed to gain a better insight in the… (more)

Subjects/Keywords: Ex vivo heart perfusion; Langendorff perfusion; Cardiac mapping; Electrophysiology

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Amesz, J. (. (2020). Cardiac mapping on ex vivo perfused porcine slaughterhouse hearts: A Langendorff perfusion protocol for epicardial mapping on isolated beating hearts. (Masters Thesis). Delft University of Technology. Retrieved from http://resolver.tudelft.nl/uuid:e2bc6f6b-ed3f-44b0-b851-74ea066a47c6

Chicago Manual of Style (16^th Edition):

Amesz, Jorik (author). “Cardiac mapping on ex vivo perfused porcine slaughterhouse hearts: A Langendorff perfusion protocol for epicardial mapping on isolated beating hearts.” 2020. Masters Thesis, Delft University of Technology. Accessed April 11, 2021. http://resolver.tudelft.nl/uuid:e2bc6f6b-ed3f-44b0-b851-74ea066a47c6.

MLA Handbook (7^th Edition):

Amesz, Jorik (author). “Cardiac mapping on ex vivo perfused porcine slaughterhouse hearts: A Langendorff perfusion protocol for epicardial mapping on isolated beating hearts.” 2020. Web. 11 Apr 2021.

Vancouver:

Amesz J(. Cardiac mapping on ex vivo perfused porcine slaughterhouse hearts: A Langendorff perfusion protocol for epicardial mapping on isolated beating hearts. [Internet] [Masters thesis]. Delft University of Technology; 2020. [cited 2021 Apr 11]. Available from: http://resolver.tudelft.nl/uuid:e2bc6f6b-ed3f-44b0-b851-74ea066a47c6.

Council of Science Editors:

Amesz J(. Cardiac mapping on ex vivo perfused porcine slaughterhouse hearts: A Langendorff perfusion protocol for epicardial mapping on isolated beating hearts. [Masters Thesis]. Delft University of Technology; 2020. Available from: http://resolver.tudelft.nl/uuid:e2bc6f6b-ed3f-44b0-b851-74ea066a47c6

University of Melbourne

29. Campbell, Bruce C. V. Acute stroke imaging: predicting response to therapy.

Degree: 2012, University of Melbourne

URL: http://hdl.handle.net/11343/37161

► Acute ischemic stroke is caused by a blocked blood vessel in the cerebral circulation. It is the most common form of stroke worldwide and a… (more)

▼ Acute ischemic stroke is caused by a blocked blood vessel in the cerebral circulation. It is the most common form of stroke worldwide and a major cause of disability and death. Treatments to re-open the blocked blood vessel and reperfuse the brain are available but their effectiveness, when applied to all patients, rapidly decreases over the first few hours after stroke onset. However, there is significant pathophysiological heterogeneity within acute stroke patients which can be revealed using advanced MRI and CT techniques. The principle of “ischemic penumbra” – hypoperfused and often non-functioning brain that will, nonetheless, potentially recover if reperfused – underlies all therapies aiming to restore blood flow in acute ischemic stroke. Perfusion-diffusion mismatch using MRI is a surrogate marker of ischemic penumbra that has been refined over the last decade. This thesis examines the validity of the mismatch paradigm and confirms the use of diffusion imaging as a reliable indicator of irreversibly damaged brain. Diffusion imaging at 24 hours (a commonly used timepoint to assess reperfusion and hemorrhage) is also established as an accurate measure of final infarct volume. This allows calculation of infarct growth as a surrogate outcome whilst minimising loss to follow-up and is a strong predictor of clinical recovery. A less predictable outcome is the proportion of hypoperfused brain that will proceed to infarction in the absence of reperfusion. Collateral blood flow is shown to be a dynamic phenomenon with alterations correlating with infarct growth. The relationship between collateral flow and perfusion-diffusion mismatch is explored. The mismatch paradigm is then translated to CT perfusion which is more widely accessible in most centres but has, until recently, lacked thorough validation. Perfusion thresholds such as Tmax>6sec translate directly to CT. The best correlate of diffusion imaging for infarct core is shown to be relative cerebral blood flow (relCBF), with the exact threshold highly dependent on the software used in the analysis. This is a shift from previous work which had suggested cerebral blood volume (CBV) was the optimal parameter. Applying mismatch-based treatment decisions in clinical practice is also examined with a comparison of simple visual assessment of mismatch with fully automated volumetric software and manual volumetric calculation. The risk of bleeding after reperfusion (hemorrhagic transformation) is the chief concern when considering reperfusion therapies. This thesis examines predictors of hemorrhage and how they may be applied in clinical practice. The ultimate aim is to move beyond simple time-based windows for treatment to an individualized treatment decision based on the particular pathophysiology revealed by imaging. The amount of potentially salvageable brain tissue can be weighed against the risk of hemorrhage to make an informed treatment decision.

Subjects/Keywords: ischemic stroke; brain imaging; perfusion; diffusion; magnetic resonance imaging; computed tomography; CT perfusion; ischemic penumbra; perfusion-diffusion mismatch; hemorrhagic transformation; thrombolysis

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Campbell, B. C. V. (2012). Acute stroke imaging: predicting response to therapy. (Doctoral Dissertation). University of Melbourne. Retrieved from http://hdl.handle.net/11343/37161

Chicago Manual of Style (16^th Edition):

Campbell, Bruce C V. “Acute stroke imaging: predicting response to therapy.” 2012. Doctoral Dissertation, University of Melbourne. Accessed April 11, 2021. http://hdl.handle.net/11343/37161.

MLA Handbook (7^th Edition):

Campbell, Bruce C V. “Acute stroke imaging: predicting response to therapy.” 2012. Web. 11 Apr 2021.

Vancouver:

Campbell BCV. Acute stroke imaging: predicting response to therapy. [Internet] [Doctoral dissertation]. University of Melbourne; 2012. [cited 2021 Apr 11]. Available from: http://hdl.handle.net/11343/37161.

Council of Science Editors:

Campbell BCV. Acute stroke imaging: predicting response to therapy. [Doctoral Dissertation]. University of Melbourne; 2012. Available from: http://hdl.handle.net/11343/37161

Vanderbilt University

30. Tierney, Jaime Elizabeth. Non-Contrast Perfusion Ultrasound Imaging for Evaluating Transarterial Chemoembolization.

Degree: PhD, Biomedical Engineering, 2019, Vanderbilt University

URL: http://hdl.handle.net/1803/15424

► Transarterial chemoembolization (TACE) treatment of liver tumors aims to reduce tumor perfusion and therefore relies on accurate perfusion imaging to monitor and evaluate treatment response.… (more)

▼ Transarterial chemoembolization (TACE) treatment of liver tumors aims to reduce tumor perfusion and therefore relies on accurate perfusion imaging to monitor and evaluate treatment response. Although effective, contrast-enhanced imaging modalities suffer from treatment-induced artifacts that necessitate late follow-up imaging, typically 4-6 weeks post-treatment. The inability to assess treatment adequacy in real-time is a potential reason for lack of treatment response. Non-contrast perfusion ultrasound is a potential solution to this problem. However, perfusion ultrasound imaging without contrast is difficult and was shown to be ineffective for TACE treatment evaluation in the past. Without contrast enhancement, blood ultrasound signal is weak compared to tissue signal, and, because perfusion occurs in the smallest, most randomly oriented vessels, perfusion Doppler signal is particularly difficult to detect. Perfusion also constitutes the slowest flow and is comparable to tissue velocity caused by patient and sonographer hand motion. This causes a spectral overlap in the slow-time frequency domain which is conventionally used for separating and removing tissue signal. There is also evidence that this motion causes an overlap in the eigen-domain, affecting more advanced principal component analysis (PCA)-based tissue filtering techniques. Therefore, despite advances in Doppler processing, beamforming, and tissue filtering, tissue clutter interference with blood has remained a barrier for realizing non-contrast perfusion ultrasound imaging for real-time TACE treatment evaluation. To address this, we developed an adaptive time-domain tissue clutter demodulation scheme to reduce the tissue clutter bandwidth prior to tissue filtering to better separate tissue and blood. The method was validated and shown to potentially allow for blood velocities to be detected that were previously thought to be impossible to visualize without contrast. The technique was improved upon and combined with other perfusion-focused advancements. Additionally, an independent component analysis (ICA)-based tissue clutter filtering method was developed and integrated with other advancements, including the adaptive demodulation method, and was shown to be superior to PCA-based techniques. Finally, the method in combination with other perfusion-focused improvements was successfully applied during a preliminary study of patients undergoing TACE. Advisors/Committee Members: Charles F. Caskey (committee member), Daniel B. Brown (committee member), Don M. Wilkes (committee member), Michael I. Miga (committee member), Brett C. Byram (Committee Chair).

Subjects/Keywords: ultrasound; adaptive demodulation; TACE; perfusion; power Doppler

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Tierney, J. E. (2019). Non-Contrast Perfusion Ultrasound Imaging for Evaluating Transarterial Chemoembolization. (Doctoral Dissertation). Vanderbilt University. Retrieved from http://hdl.handle.net/1803/15424

Chicago Manual of Style (16^th Edition):

Tierney, Jaime Elizabeth. “Non-Contrast Perfusion Ultrasound Imaging for Evaluating Transarterial Chemoembolization.” 2019. Doctoral Dissertation, Vanderbilt University. Accessed April 11, 2021. http://hdl.handle.net/1803/15424.

MLA Handbook (7^th Edition):

Tierney, Jaime Elizabeth. “Non-Contrast Perfusion Ultrasound Imaging for Evaluating Transarterial Chemoembolization.” 2019. Web. 11 Apr 2021.

Vancouver:

Tierney JE. Non-Contrast Perfusion Ultrasound Imaging for Evaluating Transarterial Chemoembolization. [Internet] [Doctoral dissertation]. Vanderbilt University; 2019. [cited 2021 Apr 11]. Available from: http://hdl.handle.net/1803/15424.

Council of Science Editors:

Tierney JE. Non-Contrast Perfusion Ultrasound Imaging for Evaluating Transarterial Chemoembolization. [Doctoral Dissertation]. Vanderbilt University; 2019. Available from: http://hdl.handle.net/1803/15424

Open Access Theses and Dissertations