subject:(Parthenogenesis)

1. Marle, Gerard. Parthenogenese : [een literatuurstudie over natuurlijke parthenogenese bij gewervelde dieren met aparte bibliografie : een experimentele studie over parthenogenetische stimulatie van muize - eicellen, de vroege in vitro ontwikkeling en pogingen tot bevruchting van deze parthenogenonten].

Degree: 1983, Erasmus University Medical Center

URL: http://hdl.handle.net/1765/37551

► textabstractDit proefschrift handelt over parthenogenese bij gewervelde dieren. Uit de literatuur blijkt dat het verschijnsel parthenogenese op vele wijzen is gedefinieerd en onderverdeeld in verschillende… (more)

Subjects/Keywords: parthenogenesis; reproduction; vertebrates

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Marle, G. (1983). Parthenogenese : [een literatuurstudie over natuurlijke parthenogenese bij gewervelde dieren met aparte bibliografie : een experimentele studie over parthenogenetische stimulatie van muize - eicellen, de vroege in vitro ontwikkeling en pogingen tot bevruchting van deze parthenogenonten]. (Doctoral Dissertation). Erasmus University Medical Center. Retrieved from http://hdl.handle.net/1765/37551

Chicago Manual of Style (16^th Edition):

Marle, Gerard. “Parthenogenese : [een literatuurstudie over natuurlijke parthenogenese bij gewervelde dieren met aparte bibliografie : een experimentele studie over parthenogenetische stimulatie van muize - eicellen, de vroege in vitro ontwikkeling en pogingen tot bevruchting van deze parthenogenonten].” 1983. Doctoral Dissertation, Erasmus University Medical Center. Accessed April 17, 2021. http://hdl.handle.net/1765/37551.

MLA Handbook (7^th Edition):

Marle, Gerard. “Parthenogenese : [een literatuurstudie over natuurlijke parthenogenese bij gewervelde dieren met aparte bibliografie : een experimentele studie over parthenogenetische stimulatie van muize - eicellen, de vroege in vitro ontwikkeling en pogingen tot bevruchting van deze parthenogenonten].” 1983. Web. 17 Apr 2021.

Vancouver:

Marle G. Parthenogenese : [een literatuurstudie over natuurlijke parthenogenese bij gewervelde dieren met aparte bibliografie : een experimentele studie over parthenogenetische stimulatie van muize - eicellen, de vroege in vitro ontwikkeling en pogingen tot bevruchting van deze parthenogenonten]. [Internet] [Doctoral dissertation]. Erasmus University Medical Center; 1983. [cited 2021 Apr 17]. Available from: http://hdl.handle.net/1765/37551.

Council of Science Editors:

Marle G. Parthenogenese : [een literatuurstudie over natuurlijke parthenogenese bij gewervelde dieren met aparte bibliografie : een experimentele studie over parthenogenetische stimulatie van muize - eicellen, de vroege in vitro ontwikkeling en pogingen tot bevruchting van deze parthenogenonten]. [Doctoral Dissertation]. Erasmus University Medical Center; 1983. Available from: http://hdl.handle.net/1765/37551

University of Kansas

2. Lutes, Aracely. Oogenesis in Unisexual Whiptail Lizards (genus Aspidoscelis).

Degree: PhD, Biochemistry & Molecular Biology, 2012, University of Kansas

URL: http://hdl.handle.net/1808/13019

► Parthenogenesis is a reproductive mode that does not require males. Though theoretically advantageous, its rarity among metazoans suggests otherwise. Paradoxically, some unisexual species within the… (more)

▼ Parthenogenesis is a reproductive mode that does not require males. Though theoretically advantageous, its rarity among metazoans suggests otherwise. Paradoxically, some unisexual species within the genus Aspidoscelis appear to be thriving by reproducing through obligate parthenogenesis. Formed via hybridization between different bisexual species, these lizards apparently reap the benefits of both hybrid vigor and higher reproductive potential. Previous studies have demonstrated the high degree of heterozygosity between generations, which contributes to the success of these lineages; a loss of heterozygosity would likely be accompanied by a decrease in fitness. How meiosis is modified in these animals is unknown. The predominant hypothesis suggests that endoreplication takes place prior to meiosis, resulting in a two-fold increase in DNA; however this has not been definitively shown. Further, the downstream modifications that result in a heterozygous gamete have merely been speculated upon. In the following thesis, meiosis within three parthenogenetic species is investigated. Quantification of DNA in germinal vesicles of the diploid parthenogen A. tesselata and the bisexual species A. gularis demonstrated that the parthenogen contains two-fold more DNA, despite equivalent somatic cell ploidy. Chiasmata were present on diplotene-stage chromosomes in both species, indicating that recombination is not bypassed. Additionally, synaptonemal complexes were found during pachytene in each species. Maintenance of heterozygosity is highly dependent on chromosome pairing in the parthenogen. Pairing between homologous chromosomes would result in a decrease; whereas pairing between identical (sister) chromosomes resulting from the additional DNA doubling event would preserve heterozygosity. Using homolog specific FISH probes in the parthenogen A. neomexicana, sister chromosome pairing is revealed. Thus, sister chromosome pairing after an additional DNA doubling allows for maintenance of heterozygosity in Aspidoscelis parthenogens. Secondly, four self-sustaining lineages of a new tetraploid species were generated from the mating between the diploid bisexual species Aspidoscelis inornata and triploid parthenogen A. exsanguis. The identity of these hybrids was confirmed by genotyping analysis. Females retain the ability to reproduce parthenogenetically through the doubling mechanism described in diploid species. These tetraploids have demonstrated how ploidy elevation hypothetically occurs in natural parthenogens. The mechanisms described in this thesis may be utilized in other parthenogens. Recent findings and future directions based on this work are presented. Advisors/Committee Members: Baumann, Peter (advisor), Albertini, David (cmtemember), Hawley, R. Scott (cmtemember), Li, Rong (cmtemember), Neaves, William (cmtemember), Zückert, Wolfram (cmtemember).

Subjects/Keywords: Biology; Aspidoscelis; Meiosis; Parthenogenesis; Reptile; Whiptail lizard

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APA (6^th Edition):

Lutes, A. (2012). Oogenesis in Unisexual Whiptail Lizards (genus Aspidoscelis). (Doctoral Dissertation). University of Kansas. Retrieved from http://hdl.handle.net/1808/13019

Chicago Manual of Style (16^th Edition):

Lutes, Aracely. “Oogenesis in Unisexual Whiptail Lizards (genus Aspidoscelis).” 2012. Doctoral Dissertation, University of Kansas. Accessed April 17, 2021. http://hdl.handle.net/1808/13019.

MLA Handbook (7^th Edition):

Lutes, Aracely. “Oogenesis in Unisexual Whiptail Lizards (genus Aspidoscelis).” 2012. Web. 17 Apr 2021.

Vancouver:

Lutes A. Oogenesis in Unisexual Whiptail Lizards (genus Aspidoscelis). [Internet] [Doctoral dissertation]. University of Kansas; 2012. [cited 2021 Apr 17]. Available from: http://hdl.handle.net/1808/13019.

Council of Science Editors:

Lutes A. Oogenesis in Unisexual Whiptail Lizards (genus Aspidoscelis). [Doctoral Dissertation]. University of Kansas; 2012. Available from: http://hdl.handle.net/1808/13019

Michigan State University

3. Namazi, Joseph. Attempts to induce parthenogenesis in plants.

Degree: MS, 1951, Michigan State University

URL: http://etd.lib.msu.edu/islandora/object/etd:12877

Subjects/Keywords: Parthenogenesis in plants

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Namazi, J. (1951). Attempts to induce parthenogenesis in plants. (Masters Thesis). Michigan State University. Retrieved from http://etd.lib.msu.edu/islandora/object/etd:12877

Chicago Manual of Style (16^th Edition):

Namazi, Joseph. “Attempts to induce parthenogenesis in plants.” 1951. Masters Thesis, Michigan State University. Accessed April 17, 2021. http://etd.lib.msu.edu/islandora/object/etd:12877.

MLA Handbook (7^th Edition):

Namazi, Joseph. “Attempts to induce parthenogenesis in plants.” 1951. Web. 17 Apr 2021.

Vancouver:

Namazi J. Attempts to induce parthenogenesis in plants. [Internet] [Masters thesis]. Michigan State University; 1951. [cited 2021 Apr 17]. Available from: http://etd.lib.msu.edu/islandora/object/etd:12877.

Council of Science Editors:

Namazi J. Attempts to induce parthenogenesis in plants. [Masters Thesis]. Michigan State University; 1951. Available from: http://etd.lib.msu.edu/islandora/object/etd:12877

Universitat de Valencia

4. Maccari, Marta. Origin and evolution of Artemia reproductive and genetic diversity .

Degree: 2017, Universitat de Valencia

URL: http://hdl.handle.net/10550/61342

► El modo de reproducción de una especie determina su diversidad genética y, a su vez, su éxito ecológico y evolutivo (Normarck et al., 2003; Simon… (more)

▼ El modo de reproducción de una especie determina su diversidad genética y, a su vez, su éxito ecológico y evolutivo (Normarck et al., 2003; Simon et al., 2003; De Meeûs et al, 2007). En una población sexual, la recombinación meiótica permite que nuevas combinaciones de genes se formen y destruyan constantemente. De hecho, las poblaciones sexuales son generalmente más diversas genéticamente en comparación con las poblaciones asexuales. Por el contrario, en un linaje estrictamente asexual, donde se supone que la mutación (con la mayoría de mutantes deletéreos) sea la única fuente de diversidad genética, se espera que la diversidad clonal de la población se reduzca en cada generación. Por esto las especies asexuales suelen ser consideradas ramas evolutivas sin salida, lo que hace presuponer que tengan una breve vida evolutiva y se extingan a corto plazo (104 - 105 generaciones) (Lynch and Gabriel, 1990). A pesar de ello, diversos estudios han demostrado que la diversidad genética de las poblaciones asexuales puede ser comparable a la de las poblaciones sexuales, si se generan repetidamente o si se producen a través de mecanismos distintos (Schwander et al., 2011, Delmotte et al., 2001, 2002, 2003). En estos casos las poblaciones asexuales producirán linajes asexuales polifiléticos muy diversos. Por ello es muy importante conocer el origen y la evolución de los linajes asexuales, y comprender cómo se genera y preserva la diversidad genética en dichos linajes. Esto nos permitirá conocer la adaptabilidad ecológica y la competitividad de las poblaciones asexuales frente a las especies sexuales emparentadas, y evaluar su potencial evolutivo (Bell, 1982; Simon et al. 2003). Artemia (Crustacea, Anostraca) es un organismo cosmopolita que vive en ecosistemas hipersalinos litorales y continentales de todo el mundo, excepto en la Antártida (Triantaphyllidis et al., 1998; Van Stappen 2002). Su importancia procede tanto de su uso práctico en acuicultura como de su aplicación científica como especie modelo en una gran variedad de investigaciones genéticas y ecológicas. Otra cualidad de este organismo, que lo hace muy interesante desde un punto de vista evolutivo, se debe a la existencia de varias especies sexuales y distintos linajes partenogenéticos de diversa ploidía (diploides, triploides, tetraploides) dentro del género (Abatzopoulos 2002), que con frecuencia coexisten. Esto nos da una oportunidad única de estudiar su diversidad genética, el origen de los linajes partenogenéticos y sus interacciones evolutivas con especies sexuales. Las poblaciones partenogenéticas diploides de Artemia en particular, uno de sus linajes mas extendidos biogeográficamente, son muy interesantes por varios aspectos. Las cepas o estirpes asexuales poliploides se reproducen por apomixis, ello implica que las divisiones de los ovocitos serán mitóticas, y que los descendientes serán verdaderos clones de la madre. Por su parte, los linajes partenogenéticos diploides se reproducen por partenogénesis automíctica. La meiosis y la recombinación génica… Advisors/Committee Members: Amat Doménech, Francisco (advisor).

Subjects/Keywords: artemia; parthenogenesis; contagious parthenogenesis; rare males; hybridization; sexuality; asexuality; reproductive isolation; phylogeography; microsatellites

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Maccari, M. (2017). Origin and evolution of Artemia reproductive and genetic diversity . (Doctoral Dissertation). Universitat de Valencia. Retrieved from http://hdl.handle.net/10550/61342

Chicago Manual of Style (16^th Edition):

Maccari, Marta. “Origin and evolution of Artemia reproductive and genetic diversity .” 2017. Doctoral Dissertation, Universitat de Valencia. Accessed April 17, 2021. http://hdl.handle.net/10550/61342.

MLA Handbook (7^th Edition):

Maccari, Marta. “Origin and evolution of Artemia reproductive and genetic diversity .” 2017. Web. 17 Apr 2021.

Vancouver:

Maccari M. Origin and evolution of Artemia reproductive and genetic diversity . [Internet] [Doctoral dissertation]. Universitat de Valencia; 2017. [cited 2021 Apr 17]. Available from: http://hdl.handle.net/10550/61342.

Council of Science Editors:

Maccari M. Origin and evolution of Artemia reproductive and genetic diversity . [Doctoral Dissertation]. Universitat de Valencia; 2017. Available from: http://hdl.handle.net/10550/61342

University of Canterbury

5. McGee, Rob. Allelism and allele sequence divergence of LOP, the locus of parthenogenesis in the model apomict Hieracium praealtum (Asteraceae).

Degree: MS, Botany, 2013, University of Canterbury

URL: http://dx.doi.org/10.26021/8231

► Apomixis, or asexual seed development, if introduced into crop species, has the potential to greatly improve global food production. Towards this goal, this study focused… (more)

▼ Apomixis, or asexual seed development, if introduced into crop species, has the potential to greatly improve global food production. Towards this goal, this study focused on uncovering the genetic mechanisms that control the parthenogenesis step within apomixis whereby fertilisation is avoided. In the model apomict, Hieracium praealtum (Asteraceae), parthenogenesis is controlled by the LOSS OF PARTHENOGENESIS (LOP) locus. Previous research showed that in addition to genomic copies of candidate genes at LOP, the genome has at least three other copies referred to as alternative alleles. The main goal of this study was to investigate four candidate genes, Genes B, X, H and Y, at LOP by generating segregation data of the alternative alleles. BAC clones containing alternative allele sequences were identified and Roche 454 pyrosequenced. These sequences were used to design alternative allele specific primers for genotyping two Hieracium praealtum polyhaploid populations (~ 300 plants). Four major conclusions were drawn from this study. First, the alternative alleles were in fact acting like alleles to the LOP alleles of Genes B, X and Y. Second, allelic sequence divergence (ASD) of the LOP alleles of Genes B and X relative to the alternative alleles, indicated a recent and separate evolutionary history. Third and, unexpectedly, recombination was detected at the LOP locus, in contrast to other apomixis loci reported in the literature. Furthermore, Gene B was found to be very closely associated with parthenogenesis in the polyhaploid population indicating that it may be essential to parthenogenesis and therefore requires further investigation. On the other hand, the absence of Genes X, Y and H, due to recombination, had no impact upon parthenogenesis. Fourth, the sequence data suggested that the LOP and alternative alleles originated from a shared common allele ancestor. It is hoped that these findings have made a significant contribution towards the future goal of introducing apomixis into crop species.

Subjects/Keywords: Apomixis; Hieracium; Hieracium praealtum; parthenogenesis; LOSS OF PARTHENOGENESIS; LOP; Allelic Sequence Divergence; ASD; repressed recombination; polyhaploids; asexual seed production; allelism

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

McGee, R. (2013). Allelism and allele sequence divergence of LOP, the locus of parthenogenesis in the model apomict Hieracium praealtum (Asteraceae). (Masters Thesis). University of Canterbury. Retrieved from http://dx.doi.org/10.26021/8231

Chicago Manual of Style (16^th Edition):

McGee, Rob. “Allelism and allele sequence divergence of LOP, the locus of parthenogenesis in the model apomict Hieracium praealtum (Asteraceae).” 2013. Masters Thesis, University of Canterbury. Accessed April 17, 2021. http://dx.doi.org/10.26021/8231.

MLA Handbook (7^th Edition):

McGee, Rob. “Allelism and allele sequence divergence of LOP, the locus of parthenogenesis in the model apomict Hieracium praealtum (Asteraceae).” 2013. Web. 17 Apr 2021.

Vancouver:

McGee R. Allelism and allele sequence divergence of LOP, the locus of parthenogenesis in the model apomict Hieracium praealtum (Asteraceae). [Internet] [Masters thesis]. University of Canterbury; 2013. [cited 2021 Apr 17]. Available from: http://dx.doi.org/10.26021/8231.

Council of Science Editors:

McGee R. Allelism and allele sequence divergence of LOP, the locus of parthenogenesis in the model apomict Hieracium praealtum (Asteraceae). [Masters Thesis]. University of Canterbury; 2013. Available from: http://dx.doi.org/10.26021/8231

6. Griffin, Clare Louise. A comparison of the ecology and behaviour of parthenogenetic and sexual taxa of the Australian skink, Menetia greyii: implications for coexistence.

Degree: 2006, Flinders University

URL: http://catalogue.flinders.edu.au./local/adt/public/adt-SFU20070202.132116

► Menetia greyii, a small Australian skink, has recently been determined to be a species complex that consists of both sexual and parthenogenetic taxa (Adams et… (more)

▼ Menetia greyii, a small Australian skink, has recently been determined to be a species complex that consists of both sexual and parthenogenetic taxa (Adams et al. 2003). In total, seven distinct taxa have been identified in the south-central region of Australia. This includes three sexual taxa, three apparent parthenogen lineages, and one lizard of uncertain status The study population occurs near Bundey Bore station in the semi-arid region of South Australia (approximately 160km north east of Adelaide). At this site, one sexual taxon (SAS) and two all-female parthenogenetic taxa (WP and RP3) were found to occur in sympatry. In a search for ecological differences, I examined spatial, thermal, physiological and morphological niche relationships in the parthenogenetic and sexual forms. Capture rates were used to determine microhabitat and macrohabitat use in the field. The use of different microhabitats and the amount of time spent occupying different exposures (sun vs. shade) were also examined under laboratory conditions. Thermal preferences, physiological performance (sprint speed ability) and daily activity periods were investigated in the laboratory. The study failed to find any major differences among the different taxa that would indicate they are partitioning resources and therefore explain how the sexual and parthenogenetic forms are coexisting. The only difference observed was that the parthenogens expressed superior sprinting ability, running faster than the sexuals over a range of temperatures. In addition, I found that sexual and parthenogenetic females within this population differed very little in their reproductive effort and output, indicating that RP3 and WP parthenogens possess a reproductive advantage over sexual females as a result of not having to produce males (Williams 1975, Maynard-Smith 1978, Bell 1982). In staged interactions between pairs of sexual and parthenogen individuals, the parthenogens were more aggressive and dominated the sexuals. As a result, the parthenogens were able to outcompete the sexuals for food items. This had serious consequences on fitness, with the sexuals losing significantly more weight than the parthenogens. All of these factors would suggest that the parthenogens should eliminate the sexuals at Bundey Bore. Despite this, the parthenogenetic females at Bundey Bore do not outnumber the sexual subpopulation. This raises the question of how the sexuals are persisting. An examination of endoparasites in the scats of parthenogen and sexual M. greyii found that WP parthenogens had significantly higher parasite prevalence than sexuals. Further to this, there is evidence of matings occurring within the study population between sexual males and WP parthenogen females with five tetraploid males being captured. Therefore, WP parthenogens may be suffering from destabilising hybridization. These factors may account for why the parthenogens (or at least the WP parthenogens) have not competitively excluded sexual M. greyii from Bundey Bore. Other possible reasons are…

Subjects/Keywords: parthenogenesis; asexual; Menetia greyii; competition

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Griffin, C. L. (2006). A comparison of the ecology and behaviour of parthenogenetic and sexual taxa of the Australian skink, Menetia greyii: implications for coexistence. (Thesis). Flinders University. Retrieved from http://catalogue.flinders.edu.au./local/adt/public/adt-SFU20070202.132116

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Griffin, Clare Louise. “A comparison of the ecology and behaviour of parthenogenetic and sexual taxa of the Australian skink, Menetia greyii: implications for coexistence.” 2006. Thesis, Flinders University. Accessed April 17, 2021. http://catalogue.flinders.edu.au./local/adt/public/adt-SFU20070202.132116.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Griffin, Clare Louise. “A comparison of the ecology and behaviour of parthenogenetic and sexual taxa of the Australian skink, Menetia greyii: implications for coexistence.” 2006. Web. 17 Apr 2021.

Vancouver:

Griffin CL. A comparison of the ecology and behaviour of parthenogenetic and sexual taxa of the Australian skink, Menetia greyii: implications for coexistence. [Internet] [Thesis]. Flinders University; 2006. [cited 2021 Apr 17]. Available from: http://catalogue.flinders.edu.au./local/adt/public/adt-SFU20070202.132116.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Griffin CL. A comparison of the ecology and behaviour of parthenogenetic and sexual taxa of the Australian skink, Menetia greyii: implications for coexistence. [Thesis]. Flinders University; 2006. Available from: http://catalogue.flinders.edu.au./local/adt/public/adt-SFU20070202.132116

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of California – Riverside

7. Tulgetske, Genet Michelle. Investigations into the Mechanisms of Wolbachia Induced Parthenogenesis and Sex Determination in the Parasitoid Wasp, Trichogramma.

Degree: Entomology, 2010, University of California – Riverside

URL: http://www.escholarship.org/uc/item/52w0b481

► In Trichogramma parasitoid wasps, infection with the intracellular bacterium, Wolbachia, alters host reproduction, inducing gamete duplication and thus the parthenogenetic production of female offspring from… (more)

▼ In Trichogramma parasitoid wasps, infection with the intracellular bacterium, Wolbachia, alters host reproduction, inducing gamete duplication and thus the parthenogenetic production of female offspring from unfertilized eggs. Little is known about the mechanisms governing sex determination in Trichogramma. Beyond outlining the basic mechanism of gamete duplication, few studies have investigated the interactions between Wolbachia and its host which allow parthenogenesis induction. This dissertation manipulates Wolbachia infection in Trichogramma kaykai to investigate the mechanisms involved in sex determination and the extent of Wolbachia's role in parthenogenesis induction. The production of males and intersexes by parthenogenetic females, and the factors contributing to their formation, provide valuable insight into mechanisms of sex determination and Wolbachia manipulation. Males and intersexes regularly appeared among the offspring of aging infected females as a result of incomplete parthenogenesis induction (Chapters 3-6). Intersexes ranged from very feminine to very masculine (Chapter 2) and flow cytometry confirmed that all were of a single genetic constitution (haploid or diploid) and therefore, not mosaics (Chapter 4). Infected females also produced occasional diploid males (Chapters 4-6). Together, these findings suggest a two-step mechanism of Wolbachia-induced parthenogenesis, requiring both gamete duplication and feminization. The complete lack of diploid males and intersexes in the absence of Wolbachia infection strongly supports this mechanism and points to a method of sex determination consistent with the imprinting model recently proposed for Nasonia vitripennis. Here, female development relies on a paternally derived sex allele to compensate for an imprinted maternal allele. The production of triploid daughters by diploid males strengthens this finding (Chapter 5). Factors modulating expression of Wolbachia-induced parthenogenesis in T. kaykai were found to include host age, host genetic background, and to a lesser extent heat (Chapter 3). Quantification of Wolbachia density (Chapter 6) revealed a clear relationship between bacterial density and wasp ploidy, with diploids harboring 7 times more than haploids. However, the relationship between Wolbachia density and sexual phenotype was not clear.

Subjects/Keywords: Biology, Entomology; Diploid males; Intersex; Parthenogenesis; Sex Determination; Trichogramma; Wolbachia

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Tulgetske, G. M. (2010). Investigations into the Mechanisms of Wolbachia Induced Parthenogenesis and Sex Determination in the Parasitoid Wasp, Trichogramma. (Thesis). University of California – Riverside. Retrieved from http://www.escholarship.org/uc/item/52w0b481

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Tulgetske, Genet Michelle. “Investigations into the Mechanisms of Wolbachia Induced Parthenogenesis and Sex Determination in the Parasitoid Wasp, Trichogramma.” 2010. Thesis, University of California – Riverside. Accessed April 17, 2021. http://www.escholarship.org/uc/item/52w0b481.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Tulgetske, Genet Michelle. “Investigations into the Mechanisms of Wolbachia Induced Parthenogenesis and Sex Determination in the Parasitoid Wasp, Trichogramma.” 2010. Web. 17 Apr 2021.

Vancouver:

Tulgetske GM. Investigations into the Mechanisms of Wolbachia Induced Parthenogenesis and Sex Determination in the Parasitoid Wasp, Trichogramma. [Internet] [Thesis]. University of California – Riverside; 2010. [cited 2021 Apr 17]. Available from: http://www.escholarship.org/uc/item/52w0b481.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Tulgetske GM. Investigations into the Mechanisms of Wolbachia Induced Parthenogenesis and Sex Determination in the Parasitoid Wasp, Trichogramma. [Thesis]. University of California – Riverside; 2010. Available from: http://www.escholarship.org/uc/item/52w0b481

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

8. Tochimara Aparecida Miyauchi. Aspectos biológicos do desenvolvimento pré- implantacional de embriões bovinos partenogenéticos ou fecundados in vitro.

Degree: 2012, Universidade Jose do Rosario Vellano

URL: http://tede.unifenas.br/tde_busca/arquivo.php?codArquivo=114

► A partenogênese tem sido descrita como um método alternativo para produzir embriões para estudos com células embrionárias, principalmente em humanos para os quais existe a… (more)

▼ A partenogênese tem sido descrita como um método alternativo para produzir embriões para estudos com células embrionárias, principalmente em humanos para os quais existe a restrição do uso de embriões fecundados. Procedimentos utilizados na partenogênese são também necessários para se produzir embriões por transferência nuclear com células somáticas nas espécies domésticas. Contudo, muitos aspectos biológicos, celulares e moleculares dos embriões partenogenéticos ainda são desconhecidos. Este estudo objetivou comparar a cinética do desenvolvimento, índice de apoptose e expressão de genes de estresse e metabolismo celular em embriões bovinos partenogenéticos e embriões fecundados in vitro. Oócitos (n=1541) obtidos de ovários de matadouro foram maturados in vitro e submetidos à ativação partenogenética (4,62 M ionomicina por 5 min seguido de 2 mM 6-DMAP por 4h) ou fecundação in vitro (2 x 106 espermatozoides/ml por 20h, com sêmen de uma única partida). Com 72h pós-ativação/fecundação (hpaf) parte dos embriões (8 células) foram congelados para posterior análise da expressão gênica e outra parte foi separada em grupos de alto ou baixo potencial de desenvolvimento: Part≥8 - embriões partenogenéticos com 8 ou mais células (alto potencial de desenvolvimento); Part<8 - embriões com menos de 8 células (baixo potencial de desenvolvimento); FIV≥8 - embriões fecundados in vitro com 8 ou mais células; e FIV<8: embriões com menos de 8 células. Os embriões foram cultivados em meio CR2aa com 2,5% SFB em 5%CO2, 5%O2, 90% N2 a 38,5C e avaliadas as taxas de blastocistos com 168hpaf (D7) e 196hpaf (D8). Embriões com 8 células obtidos após 72hpfa foram analisados quanto à expressão gênica. Blastocistos em D8 foram fixados e posteriormente foram avaliados pela técnica de TUNEL o índice apoptótico. Os dados foram comparados por análise de variância e as médias por teste de Student Newman Keuls. A expressão gênica foi avaliada pelo software REST. Os valores são mostrados como médiaerro padrão. Embriões com 8 ou mais células produziram maiores (P<0,01) taxas de blastocistos no D7 e D8 do que os embriões com menos de oitos células mostrando seu maior potencial de desenvolvimento, independentemente se foram partenogenéticos ou fecundados. Embriões do grupo Part≥8 apresentaram maior (P<0,05) taxa de blastocistos no D7 (63,63,4%) que os FIV≥8 (45,38,9%), porém a taxa no D8 foi semelhante (56,73,0% e 44,28,9% para Part≥8 e FIV≥8, respectivamente; (P<0,05). Não houve diferença (P<0,05) quanto às taxas de blastocistos no D7 e D8 entre embriões com menos de 8 células oriundos da partenogênese ou fecundação. Não houve diferença quanto ao número total de células (92,03,4; 102,304,8), células apoptóticas (10,81,22; 9,51,07) e índice apoptótico (11,41,27; 9,81,8) nos blastocistos partenogenéticos e FIV, respectivamente. Nos embriões com 8 células analisados, houve subexpressão dos genes DNAJB1, HSPA1L, HSF1, GLUT1 nos Part em relação aos FIV, enquanto o HSF2 esteve sobrexpresso nos Part quando comparado aos FIV.… Advisors/Committee Members: Carlos Antônio de Carvalho Fernandes, Eduardo Kenji Nunes Arashiro, Luiz Sérgio de Almeida Camargo.

Subjects/Keywords: partenogênese; expressão gênica; embrião; bovino; EMBRIOLOGIA; parthenogenesis; gene expression; embryo; bovine

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Miyauchi, T. A. (2012). Aspectos biológicos do desenvolvimento pré- implantacional de embriões bovinos partenogenéticos ou fecundados in vitro. (Thesis). Universidade Jose do Rosario Vellano. Retrieved from http://tede.unifenas.br/tde_busca/arquivo.php?codArquivo=114

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Miyauchi, Tochimara Aparecida. “Aspectos biológicos do desenvolvimento pré- implantacional de embriões bovinos partenogenéticos ou fecundados in vitro.” 2012. Thesis, Universidade Jose do Rosario Vellano. Accessed April 17, 2021. http://tede.unifenas.br/tde_busca/arquivo.php?codArquivo=114.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Miyauchi, Tochimara Aparecida. “Aspectos biológicos do desenvolvimento pré- implantacional de embriões bovinos partenogenéticos ou fecundados in vitro.” 2012. Web. 17 Apr 2021.

Vancouver:

Miyauchi TA. Aspectos biológicos do desenvolvimento pré- implantacional de embriões bovinos partenogenéticos ou fecundados in vitro. [Internet] [Thesis]. Universidade Jose do Rosario Vellano; 2012. [cited 2021 Apr 17]. Available from: http://tede.unifenas.br/tde_busca/arquivo.php?codArquivo=114.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Miyauchi TA. Aspectos biológicos do desenvolvimento pré- implantacional de embriões bovinos partenogenéticos ou fecundados in vitro. [Thesis]. Universidade Jose do Rosario Vellano; 2012. Available from: http://tede.unifenas.br/tde_busca/arquivo.php?codArquivo=114

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Penn State University

9. Vasquez, Kate. Post-embryonic mutations are inherited in the next generation of corals.

Degree: 2019, Penn State University

URL: https://submit-etda.libraries.psu.edu/catalog/17223klv9

► Increasingly frequent wide-spread coral bleaching caused by rising seawater temperatures is a strong selective force that is changing the genetic composition of surviving coral populations.… (more)

▼ Increasingly frequent wide-spread coral bleaching caused by rising seawater temperatures is a strong selective force that is changing the genetic composition of surviving coral populations. Successful sexual reproduction of these survivors is the most commonly-studied means by which species can adapt to rapid changes. However, the dominant mode of reproduction in corals is often asexual via budding of polyps in a colony or breakage and reattachment of pieces of the colony. In this way, coral genets can persist for hundreds to thousands of years while accumulating a substantial pool of post-embryonic mutations. Here we show through the analysis of genetic marker inheritance that Caribbean Elkhorn coral (Acropora palmata) colonies transfer post-embryonic mutations to the next generation. Furthermore, we show that a subset of colonies produce parthenogenetic offspring that are not completely identical to their parent due to recombination. In this way, colonies that are producing parthenogenetic larvae may still contribute to future reef genetic diversity. With the need to implement management strategies that create genetically diverse reefs, the necessity for population structure software to produce accurate results is more than ever important. The software program STRUCTURE relies on a Bayesian iterative clustering algorithm and is one of the most cited tools for determining genetic population structure. The program uses multi-locus genotype data to cluster genetic samples. Among the most common methods for inferring the optimal number of clusters is the ΔK method. However, a recent study relying on modeled data demonstrated that this method may have a downward bias in its estimation of K and may also be sensitive to uneven sampling. If this finding holds for empirical datasets, conclusions about the scale of gene flow may have to be revised for a large number of studies. Advisors/Committee Members: Iliana Brigitta Baums, Thesis Advisor/Co-Advisor, Stephen Wade Schaeffer, Committee Member, Anton Nekrutenko, Committee Member, Stephen Wade Schaeffer, Program Head/Chair.

Subjects/Keywords: Post-embryonic mutation; parthenogenesis; Acropora palmata; Microsatellite; Single Nucleotide Polymorphism

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APA (6^th Edition):

Vasquez, K. (2019). Post-embryonic mutations are inherited in the next generation of corals. (Thesis). Penn State University. Retrieved from https://submit-etda.libraries.psu.edu/catalog/17223klv9

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Vasquez, Kate. “Post-embryonic mutations are inherited in the next generation of corals.” 2019. Thesis, Penn State University. Accessed April 17, 2021. https://submit-etda.libraries.psu.edu/catalog/17223klv9.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Vasquez, Kate. “Post-embryonic mutations are inherited in the next generation of corals.” 2019. Web. 17 Apr 2021.

Vancouver:

Vasquez K. Post-embryonic mutations are inherited in the next generation of corals. [Internet] [Thesis]. Penn State University; 2019. [cited 2021 Apr 17]. Available from: https://submit-etda.libraries.psu.edu/catalog/17223klv9.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Vasquez K. Post-embryonic mutations are inherited in the next generation of corals. [Thesis]. Penn State University; 2019. Available from: https://submit-etda.libraries.psu.edu/catalog/17223klv9

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Iowa

10. Hanson, Sara Jeanette. The molecular evolution of reproduction in animals: insights from sexual and asexual rotifers.

Degree: PhD, Genetics, 2013, University of Iowa

URL: https://ir.uiowa.edu/etd/1618

► Sex and meiosis are ubiquitous in eukaryotes as the primary mode of reproduction. This suggests that despite the theoretical energetic advantages of asexual reproduction,… (more)

▼ Sex and meiosis are ubiquitous in eukaryotes as the primary mode of reproduction. This suggests that despite the theoretical energetic advantages of asexual reproduction, organisms capable of sexual reproduction are at a much greater long-term evolutionary advantage. Rotifers, a group of microinvertebrates, offer unique opportunities to examine the evolution of sex due to their extensive proliferation, successful adaptation to a wide variety of ecological niches, and the diversity of reproductive modes represented in the group. The cyclically parthenogenetic monogonont rotifers have overcome constraints on the loss of sexual reproduction in order to frequently transition between sexual and asexual generations, making them a powerful system with which to address the maintenance of sex in animals. Obligately asexual bdelloid rotifers appear to have thrived without sex for tens of millions of years, a period of time much longer than expected given the hypothesized advantages of sexual reproduction. However, the molecular nature of sex and parthenogenesis is poorly understood in any rotifer species. To expand our knowledge of the molecular mechanisms of monogonont reproduction, we sequenced genomes of two distantly related species, Brachionus calyciflorus and Brachionus manjavacas and identified over 80 homologs for genes involved in meiotic processes. Several of these genes have undergone duplication events specific to the monogonont lineage, including genes with known roles in regulation of cell cycle transitions during meiosis. In addition, global gene expression patterns were determined using obligate parthenogenetic (OP) and cyclical parthenogenetic (CP) strains of B. calyciflorus. Quantitative comparison of expression between these strains revealed differentially expressed genes specific to sexual and asexual reproduction in this species, including genes related to dormancy/resting egg formation, meiosis, and hormone signaling pathways that are thought to be involved in the induction of sexual reproduction in monogononts. Finally, we analyzed gene expression in bdelloid rotifers for evidence of sexual reproduction or the utilization of meiotic genes under conditions inducing high levels of recombination. Through this work, we have established molecular markers for sexuality and asexuality in monogonont rotifers, and used these markers to evaluate reproduction in bdelloids. The data generated specifically allows for more informed analyses of the evolution of cyclical parthenogenesis and rotifer reproduction. Furthermore, this work extends the use of monogononts as a model system for addressing broader questions regarding the evolution of sexual reproduction. Advisors/Committee Members: Logsdon, John M., Jr. (supervisor).

Subjects/Keywords: Cyclical Parthenogenesis; Genomics; Phylogenetics; Rotifer; Sexual Reproduction; Transcriptomics; Genetics

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Hanson, S. J. (2013). The molecular evolution of reproduction in animals: insights from sexual and asexual rotifers. (Doctoral Dissertation). University of Iowa. Retrieved from https://ir.uiowa.edu/etd/1618

Chicago Manual of Style (16^th Edition):

Hanson, Sara Jeanette. “The molecular evolution of reproduction in animals: insights from sexual and asexual rotifers.” 2013. Doctoral Dissertation, University of Iowa. Accessed April 17, 2021. https://ir.uiowa.edu/etd/1618.

MLA Handbook (7^th Edition):

Hanson, Sara Jeanette. “The molecular evolution of reproduction in animals: insights from sexual and asexual rotifers.” 2013. Web. 17 Apr 2021.

Vancouver:

Hanson SJ. The molecular evolution of reproduction in animals: insights from sexual and asexual rotifers. [Internet] [Doctoral dissertation]. University of Iowa; 2013. [cited 2021 Apr 17]. Available from: https://ir.uiowa.edu/etd/1618.

Council of Science Editors:

Hanson SJ. The molecular evolution of reproduction in animals: insights from sexual and asexual rotifers. [Doctoral Dissertation]. University of Iowa; 2013. Available from: https://ir.uiowa.edu/etd/1618

University of Melbourne

11. Gagliardi, Bryant Samuel. Differentiating pollutant-induced effects from non-contaminant stress responses in aquatic midges (Diptera: Chironomidae).

Degree: 2017, University of Melbourne

URL: http://hdl.handle.net/11343/193008

► Ecotoxicology is the study of environmental contaminants and their effects on organisms. Laboratory ecotoxicology typically investigates contaminant responses in standard, model test organisms. There is… (more)

▼ Ecotoxicology is the study of environmental contaminants and their effects on organisms. Laboratory ecotoxicology typically investigates contaminant responses in standard, model test organisms. There is often a lack of understanding of the role of non-contaminant factors in inducing these stress effects. In the freshwater model insect Chironomus (Diptera: Chironomidae), deformed larvae have been observed since the early 1970s, and these have been proposed as a potentially useful sublethal contaminant response for monitoring studies. However, despite decades of research, the causal links between contaminants and deformities are uncertain, limiting the application of this endpoint in ecotoxicology. There is also a poor understanding of the role of non-contaminant environmental stressors in inducing deformities. Additionally, inbreeding depression has been shown to be a widespread compromising factor in Chironomus assays investigating lethality, reproduction and deformity effects. Inbreeding in cultures appears to be a difficult issue to resolve. The aims of this thesis were to clarify the causes of larval deformities in Chironomus, considering chemical and non-chemical causal factors, and to investigate a potential solution to the widespread issues surrounding inbreeding in chironomid ecotoxicology. Firstly, I conducted a meta-analysis of published laboratory Chironomus deformity assays, characterising and quantifying the inconsistency of published data across > 45 years of research. I also aimed to diagnose potential causes of this inconsistency between assays, focussing on the influence of potential extraneous laboratory stressors in assays, and mortality of larvae in assays as a potential confounding variable. Significant inconsistency was observed across published assays, and mortality effects and extraneous stressors were found to be potentially contributing to this inconsistency. Secondly, I conducted laboratory assays investigating chemical and non-chemical deformity causes in the Australian model chironomid C. tepperi. I aimed to address the key issues identified in the meta-analysis as limiting to the understanding of deformity causes. These issues were the presence of extraneous laboratory stressors (as indicated by high control deformity incidences), potential confounding of sublethal deformity assays by mortality (avoided by ensuring sublethal conditions), and the widespread failure to consider non-contaminant causes (addressed by testing non-chemical factors: food limitation and onset of pupation). None of the tested factors were observed to induce significant deformity effects. Finally, I aimed to investigate a potential solution to prevalent inbreeding issues in ecotoxicology associated with the use of Chironomus as a model insect. This involved developing a standard culturing methodology, and conducting preliminary toxicological investigations for the cosmopolitan nonmodel chironomid Paratanytarsus grimmii. This species reproduces asexually, by apomictic parthenogenesis. No mating takes…

Subjects/Keywords: Chironomidae; deformities; inbreeding depression; survivorship bias; parthenogenesis; aquatic ecotoxicology

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Gagliardi, B. S. (2017). Differentiating pollutant-induced effects from non-contaminant stress responses in aquatic midges (Diptera: Chironomidae). (Doctoral Dissertation). University of Melbourne. Retrieved from http://hdl.handle.net/11343/193008

Chicago Manual of Style (16^th Edition):

Gagliardi, Bryant Samuel. “Differentiating pollutant-induced effects from non-contaminant stress responses in aquatic midges (Diptera: Chironomidae).” 2017. Doctoral Dissertation, University of Melbourne. Accessed April 17, 2021. http://hdl.handle.net/11343/193008.

MLA Handbook (7^th Edition):

Gagliardi, Bryant Samuel. “Differentiating pollutant-induced effects from non-contaminant stress responses in aquatic midges (Diptera: Chironomidae).” 2017. Web. 17 Apr 2021.

Vancouver:

Gagliardi BS. Differentiating pollutant-induced effects from non-contaminant stress responses in aquatic midges (Diptera: Chironomidae). [Internet] [Doctoral dissertation]. University of Melbourne; 2017. [cited 2021 Apr 17]. Available from: http://hdl.handle.net/11343/193008.

Council of Science Editors:

Gagliardi BS. Differentiating pollutant-induced effects from non-contaminant stress responses in aquatic midges (Diptera: Chironomidae). [Doctoral Dissertation]. University of Melbourne; 2017. Available from: http://hdl.handle.net/11343/193008

Massey University

12. Nakano, Mari. Male mate choice in the stick insect Clitarchus hookeri : sexual vs. parthenogenetic females.

Degree: MS, Zoology, 2018, Massey University

URL: http://hdl.handle.net/10179/15286

► Learning about reproductive strategies in animals is an important step for understanding the evolution of species. New Zealand stick insect, Clitarchus hookeri include both sexual… (more)

Subjects/Keywords: Stick insects; New Zealand; Reproduction; Courtship of animals; Parthenogenesis in animals|

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APA (6^th Edition):

Nakano, M. (2018). Male mate choice in the stick insect Clitarchus hookeri : sexual vs. parthenogenetic females. (Masters Thesis). Massey University. Retrieved from http://hdl.handle.net/10179/15286

Chicago Manual of Style (16^th Edition):

Nakano, Mari. “Male mate choice in the stick insect Clitarchus hookeri : sexual vs. parthenogenetic females.” 2018. Masters Thesis, Massey University. Accessed April 17, 2021. http://hdl.handle.net/10179/15286.

MLA Handbook (7^th Edition):

Nakano, Mari. “Male mate choice in the stick insect Clitarchus hookeri : sexual vs. parthenogenetic females.” 2018. Web. 17 Apr 2021.

Vancouver:

Nakano M. Male mate choice in the stick insect Clitarchus hookeri : sexual vs. parthenogenetic females. [Internet] [Masters thesis]. Massey University; 2018. [cited 2021 Apr 17]. Available from: http://hdl.handle.net/10179/15286.

Council of Science Editors:

Nakano M. Male mate choice in the stick insect Clitarchus hookeri : sexual vs. parthenogenetic females. [Masters Thesis]. Massey University; 2018. Available from: http://hdl.handle.net/10179/15286

13. Gangopadhyay, Debnirmalya. Application of artificial parthenogenesis in the development of breeds of the mulberry silkworm, Bombyx mori L;.

Degree: 2007, University of Mysore

URL: http://shodhganga.inflibnet.ac.in/handle/10603/39860

newline

Advisors/Committee Members: Singh, Ravindra.

Subjects/Keywords: Ameiotic Parthenogenesis; Artificial Parthenogenesis; Bivoltine F1 Hybrids; Bombyx mori L.; Mulberry Silkworm

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Gangopadhyay, D. (2007). Application of artificial parthenogenesis in the development of breeds of the mulberry silkworm, Bombyx mori L;. (Thesis). University of Mysore. Retrieved from http://shodhganga.inflibnet.ac.in/handle/10603/39860

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Gangopadhyay, Debnirmalya. “Application of artificial parthenogenesis in the development of breeds of the mulberry silkworm, Bombyx mori L;.” 2007. Thesis, University of Mysore. Accessed April 17, 2021. http://shodhganga.inflibnet.ac.in/handle/10603/39860.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Gangopadhyay, Debnirmalya. “Application of artificial parthenogenesis in the development of breeds of the mulberry silkworm, Bombyx mori L;.” 2007. Web. 17 Apr 2021.

Vancouver:

Gangopadhyay D. Application of artificial parthenogenesis in the development of breeds of the mulberry silkworm, Bombyx mori L;. [Internet] [Thesis]. University of Mysore; 2007. [cited 2021 Apr 17]. Available from: http://shodhganga.inflibnet.ac.in/handle/10603/39860.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Gangopadhyay D. Application of artificial parthenogenesis in the development of breeds of the mulberry silkworm, Bombyx mori L;. [Thesis]. University of Mysore; 2007. Available from: http://shodhganga.inflibnet.ac.in/handle/10603/39860

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

14. Takase, Kikuya. Apoptosis in the Degeneration Process of Unfertilized Mouse Ova : マウス未受精卵の変性過程におけるアポトーシスの検討.

Degree: 1995, Kindai University / 近畿大学

URL: http://id.nii.ac.jp/1391/00002114/

►

[Abstract] In mammals, ova which are not fertilized undergo degeneration. Thus, it seems that the ovum is programmed to die unless fertilization andembryogenesis occurs, but… (more)

Subjects/Keywords: ovum; polar body; apoptosis; fertilization; parthenogenesis

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APA (6^th Edition):

Takase, . K. (1995). Apoptosis in the Degeneration Process of Unfertilized Mouse Ova : マウス未受精卵の変性過程におけるアポトーシスの検討. (Thesis). Kindai University / 近畿大学. Retrieved from http://id.nii.ac.jp/1391/00002114/

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Takase, Kikuya. “Apoptosis in the Degeneration Process of Unfertilized Mouse Ova : マウス未受精卵の変性過程におけるアポトーシスの検討.” 1995. Thesis, Kindai University / 近畿大学. Accessed April 17, 2021. http://id.nii.ac.jp/1391/00002114/.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Takase, Kikuya. “Apoptosis in the Degeneration Process of Unfertilized Mouse Ova : マウス未受精卵の変性過程におけるアポトーシスの検討.” 1995. Web. 17 Apr 2021.

Vancouver:

Takase K. Apoptosis in the Degeneration Process of Unfertilized Mouse Ova : マウス未受精卵の変性過程におけるアポトーシスの検討. [Internet] [Thesis]. Kindai University / 近畿大学; 1995. [cited 2021 Apr 17]. Available from: http://id.nii.ac.jp/1391/00002114/.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Takase K. Apoptosis in the Degeneration Process of Unfertilized Mouse Ova : マウス未受精卵の変性過程におけるアポトーシスの検討. [Thesis]. Kindai University / 近畿大学; 1995. Available from: http://id.nii.ac.jp/1391/00002114/

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Mississippi State University

15. Ramachandran, Reshma. Normal fertilization and factors influencing the process of parthenogenesis in Chinese painted quail.

Degree: PhD, Poultry Science, 2018, Mississippi State University

URL: http://sun.library.msstate.edu/ETD-db/theses/available/etd-06192018-135228/ ;

► In the modern poultry industry, intense genetic selection for meat production has negatively influenced the reproductive performance of commercial birds. Parthenogenesis, embryonic development in unfertilized… (more)

▼ In the modern poultry industry, intense genetic selection for meat production has negatively influenced the reproductive performance of commercial birds. Parthenogenesis, embryonic development in unfertilized eggs without any sperm-egg interactions, is known to hinder the normal fertilization process and could be one of the reasons for this reduced reproductive performance in the poultry industry. Therefore, the overall objective of this research was to gain a better understanding of the process of parthenogenesis using Chinese painted quail as the model. Studies on Chinese painted quail reproduction revealed that they are very inefficient in sustained sperm storage and that number of sperm penetrating the egg and subsequent embryonic development potentially alter egg transit time through the oviduct. This poor sperm storage capacity and high sperm-egg interaction requirement might be responsible for the occurrence of parthenogenesis in this species; and in fact, this makes Chinese painted quail an excellent choice for parthenogenesis research. Further, dams selected for parthenogenesis as well as embryonic development, including parthenogen size, alter egg components by possibly delaying the transit time of the egg through the oviduct. Also, both dams and sires selected for the parthenogenesis trait appear to influence their progenies performance, including 1st wk mortality and occurrence of parthenogenesis. Additionally, vaccination of virgin hens with live pigeon pox virus increases parthenogenesis as well as parthenogen size and livability by the direct action of the virus on the embryo. Moreover, live Newcastle disease virus under in vitro conditions was found to have similar effects on the embryo. Because parthenogenesis exists in the modern poultry industry, even the accidental selection of the trait in either males or females could have a negative impact on overall chick production and performance. Also, as vaccination is a routine practice in the industry, it is possible that vaccination of birds that carry the trait will reduce fertility and hatchability due to enhanced parthenogenesis. Overall, currently it appears that, parthenogenesis is adversely affecting the poultry industry; and therefore, additional research on the accurate determination of losses in the poultry industry due to parthenogenesis could further benefit the industry. Advisors/Committee Members: Christopher D. McDaniel (chair), E. David Peebles (committee member), Aaron S. Kiess (committee member), Kelley G.S.Wamsley (committee member).

Subjects/Keywords: Parthenogenesis; sperm-egg interactions; egg components; embryo development; pigeon pox virus; newcastle disease virus; poultry

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APA (6^th Edition):

Ramachandran, R. (2018). Normal fertilization and factors influencing the process of parthenogenesis in Chinese painted quail. (Doctoral Dissertation). Mississippi State University. Retrieved from http://sun.library.msstate.edu/ETD-db/theses/available/etd-06192018-135228/ ;

Chicago Manual of Style (16^th Edition):

Ramachandran, Reshma. “Normal fertilization and factors influencing the process of parthenogenesis in Chinese painted quail.” 2018. Doctoral Dissertation, Mississippi State University. Accessed April 17, 2021. http://sun.library.msstate.edu/ETD-db/theses/available/etd-06192018-135228/ ;.

MLA Handbook (7^th Edition):

Ramachandran, Reshma. “Normal fertilization and factors influencing the process of parthenogenesis in Chinese painted quail.” 2018. Web. 17 Apr 2021.

Vancouver:

Ramachandran R. Normal fertilization and factors influencing the process of parthenogenesis in Chinese painted quail. [Internet] [Doctoral dissertation]. Mississippi State University; 2018. [cited 2021 Apr 17]. Available from: http://sun.library.msstate.edu/ETD-db/theses/available/etd-06192018-135228/ ;.

Council of Science Editors:

Ramachandran R. Normal fertilization and factors influencing the process of parthenogenesis in Chinese painted quail. [Doctoral Dissertation]. Mississippi State University; 2018. Available from: http://sun.library.msstate.edu/ETD-db/theses/available/etd-06192018-135228/ ;

16. Tochimara Aparecida Miyauchi. Aspectos biológicos do desenvolvimento pré- implantacional de embriões bovinos partenogenéticos ou fecundados in vitro.

Degree: 2012, Universidade Jose do Rosario Vellano

URL: http://tede.unifenas.br/tde_busca/arquivo.php?codArquivo=130

►

Parthenogenesis has been described as an alternative method to produce embryos for studies with embryonic cells, particularly in humans which have a restriction of fertilized… (more)

▼

Parthenogenesis has been described as an alternative method to produce embryos for studies with embryonic cells, particularly in humans which have a restriction of fertilized embryos. Procedures used in parthenogenesis are also required to produce embryos by somatic cell nuclear transfer in domestic species. However, many biological, cellular and molecular aspects of parthenogenetic embryos are still unknown. This study aimed to compare the kinetics of development, apoptosis rate and gene expression of stress and celular metabolism in bovine parthenogenetic embryos and embryos fertilized in vitro. Oocytes (n = 1541) obtained from slaughterhouse ovaries were matured in vitro and submitted to parthenogenetic activation (4.62 M ionomycin for 5 min followed by 2 mM 6-DMAP for 4 h) or in vitro fertilization (2 x 106 sperm/mL for 20h with semen from a single departure). 72h postactivation/fertilization (hpaf), embryos (8-cell) were frozen for subsequent analysis of gene expression and another part has been separated into groups of high or low potential of development: Part ≥ 8 - parthenogenetic embryos with 8 or more cells (high development potential); Part <8 - embryos less than 8 cells (low development potential); ≥ 8 IVF - in vitro fertilized embryos with 8 or more cells; and IVF <8 - embryos with less than 8 cells. Embryos were cultured in CR2aa medium with 2.5% BFS in 5% CO2, 5% O2, 90% N2 at 38.5 C and were evaluated rates of blastocyst 168hpaf (D7) and 196hpaf (D8). 8-cell embryos obtained after 72hpfa were analyzed for gene expression. D8 blastocysts were fixed and subsequently apoptotic index was analyzed by TUNEL. Data were compared by analysis of variance and means by Student Newman Keuls test. Gene expression was evaluated by REST software. Values are shown as mean standard error. Embryos with 8 or more cells produced higher (P <0.01) blastocysts rates in D7 and D8 compared to embryos with less than eight cells, showing its greatest development potential, regardless if they were parthenogenetic or fertilized. Embryos of Part ≥ 8 group had higher (P <0.05) blastocyst rate in D7 (63.6 3.4%) than IVF ≥ 8 (45.3 8.9%), but at D8 rate was similar (56.7 3.0% and 44.2 8.9% for Part ≥ 8 and IVF ≥ 8, respectively; P <0.05). There was no difference (P <0.05) on blastocyst rates at D7 and D8 between embryos with less than 8 cells derived from parthenogenesis or fertilization. There was no difference in total cell number (92.0 3.4, 102.30 4.8), apoptotic cells (10.8 1.22, 9.5 1.07) and apoptotic index (11.4 1.27, 9.8 1.8) for parthenogenetic and IVF blastocysts, respectively. In 8-cell embryos analyzed, there was a subexpression of genes DNAJB1, HSPA1L, HSF1 and GLUT1 for Part in relation to FIV, while HSF2 was overexpressed in Part compared to FIV. In conclusion, bovine parthenogenetic embryos differ to embryos fertilized in vitro in hability of preimplantation development in vitro and gene expression, which may limit the use of parthenogenesis in studies of embryonic development.

A…

Advisors/Committee Members: Carlos Antônio de Carvalho Fernandes, Eduardo Kenji Nunes Arashiro, Luiz Sérgio de Almeida Camargo.

Subjects/Keywords: partenogênese; expressão gênica; embrião; bovino; MEDICINA VETERINARIA; parthenogenesis; gene expression; embryo; bovine

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Miyauchi, T. A. (2012). Aspectos biológicos do desenvolvimento pré- implantacional de embriões bovinos partenogenéticos ou fecundados in vitro. (Thesis). Universidade Jose do Rosario Vellano. Retrieved from http://tede.unifenas.br/tde_busca/arquivo.php?codArquivo=130

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Miyauchi, Tochimara Aparecida. “Aspectos biológicos do desenvolvimento pré- implantacional de embriões bovinos partenogenéticos ou fecundados in vitro.” 2012. Thesis, Universidade Jose do Rosario Vellano. Accessed April 17, 2021. http://tede.unifenas.br/tde_busca/arquivo.php?codArquivo=130.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Miyauchi, Tochimara Aparecida. “Aspectos biológicos do desenvolvimento pré- implantacional de embriões bovinos partenogenéticos ou fecundados in vitro.” 2012. Web. 17 Apr 2021.

Vancouver:

Miyauchi TA. Aspectos biológicos do desenvolvimento pré- implantacional de embriões bovinos partenogenéticos ou fecundados in vitro. [Internet] [Thesis]. Universidade Jose do Rosario Vellano; 2012. [cited 2021 Apr 17]. Available from: http://tede.unifenas.br/tde_busca/arquivo.php?codArquivo=130.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Miyauchi TA. Aspectos biológicos do desenvolvimento pré- implantacional de embriões bovinos partenogenéticos ou fecundados in vitro. [Thesis]. Universidade Jose do Rosario Vellano; 2012. Available from: http://tede.unifenas.br/tde_busca/arquivo.php?codArquivo=130

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Guelph

17. Gonzalez Grajales, Leslie Antonio. Development of bison embryos produced by parthenogenesis, interspecies somatic cell nuclear transfer, and ooplasm transfer.

Degree: PhD, Department of Biomedical Sciences, 2015, University of Guelph

URL: https://atrium.lib.uoguelph.ca/xmlui/handle/10214/8638

► The success of reproductive biotechnologies including in vitro embryo production in bison species has been low although several techniques, such as interspecies somatic cell nuclear… (more)

▼ The success of reproductive biotechnologies including in vitro embryo production in bison species has been low although several techniques, such as interspecies somatic cell nuclear transfer (iSCNT) hold promise of efficacy in this species. However, interactions between cattle ooplasmic components and bison nuclear structures influence early development in bison iSCNT embryos. Differences in ATP content, incidence of apoptosis, and gene expression between cattle SCNT and bison iSCNT embryos suggest altered interactions in nuclear and mitochondrial genomes, although no differences in developmental rates were observed. A cattle IVM maturation and IVC system were used to determine the developmental potential of plains bison oocytes as cytoplasts. Maturation and developmental rates were similar between cattle and plains bison. The majority of oocytes in both groups reached metaphase meiosis II at 24 hours post maturation and the 8-16 cell stage on day 4. Although total blastocyst rate was similar between cattle and plains bison, embryos in the latter group consistently reached the blastocyst stage 24 to 48 hours after cattle parthenogenic embryos. Modifications to previously described ooplasm transfer techniques were performed to decrease the number of micromanipulations needed when combined with SCNT. The effects of ooplasm transfer into cattle SCNT and iSCNT plains bison embryos was investigated and demonstrated that supplementation of ooplasm matching the genetic origin of the somatic cell in reconstructed SCNT or iSCNT embryos had no effects on development, ATP content, and gene expression profiles at the 8-16 cell stage. Finally, embryo quality between vitrified-thawed wood bison blastocysts produced in vivo and wood bison iSCNT blastocysts generated in vitro was investigated. The percentage of in vivo-derived embryos showing normal morphology was only 20% of that in vitro. Although in vivo embryos expanded, initiated the hatching process, and had higher total cell number, no differences were found in apoptosis incidence when compared to iSCNT embryos. Delayed development at the blastocyst stage was observed in iSCNT wood bison embryos when compared to in vivo embryos. The present study demostrated developmental alterations in iSCNT bison embryos, no effects on development by ooplasm supplementation, and compromised competence of in vivo-derived wood bison embryos. Advisors/Committee Members: King, W. Allan (advisor).

Subjects/Keywords: bison; embryo; interspecies somatic cell nuclear transfer; parthenogenesis; reproductive biotechnologies; in vitro embryo production

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Gonzalez Grajales, L. A. (2015). Development of bison embryos produced by parthenogenesis, interspecies somatic cell nuclear transfer, and ooplasm transfer. (Doctoral Dissertation). University of Guelph. Retrieved from https://atrium.lib.uoguelph.ca/xmlui/handle/10214/8638

Chicago Manual of Style (16^th Edition):

Gonzalez Grajales, Leslie Antonio. “Development of bison embryos produced by parthenogenesis, interspecies somatic cell nuclear transfer, and ooplasm transfer.” 2015. Doctoral Dissertation, University of Guelph. Accessed April 17, 2021. https://atrium.lib.uoguelph.ca/xmlui/handle/10214/8638.

MLA Handbook (7^th Edition):

Gonzalez Grajales, Leslie Antonio. “Development of bison embryos produced by parthenogenesis, interspecies somatic cell nuclear transfer, and ooplasm transfer.” 2015. Web. 17 Apr 2021.

Vancouver:

Gonzalez Grajales LA. Development of bison embryos produced by parthenogenesis, interspecies somatic cell nuclear transfer, and ooplasm transfer. [Internet] [Doctoral dissertation]. University of Guelph; 2015. [cited 2021 Apr 17]. Available from: https://atrium.lib.uoguelph.ca/xmlui/handle/10214/8638.

Council of Science Editors:

Gonzalez Grajales LA. Development of bison embryos produced by parthenogenesis, interspecies somatic cell nuclear transfer, and ooplasm transfer. [Doctoral Dissertation]. University of Guelph; 2015. Available from: https://atrium.lib.uoguelph.ca/xmlui/handle/10214/8638

Uniwersytet im. Adama Mickiewicza w Poznaniu

18. Piosik, Łukasz. Obcy gatunkowo pyłek i czynnik chemiczny w indukcji rozwoju zarodków haploidalnych u Lactuca sativa L.

Degree: 2014, Uniwersytet im. Adama Mickiewicza w Poznaniu

URL: http://hdl.handle.net/10593/12207

► Sałata siewna (Lactuca sativa) jest popularną oraz cenną z ekonomicznego punktu widzenia rośliną warzywną. Głównym celem tej pracy było opracowanie efektywnej metody haploidyzacji Lactuca sativa,… (more)

Subjects/Keywords: Krzyżowanie międzyrodzajowe; Intergeneric crossing; Lactuca sativa; Partenogeneza; Parthenogenesis; Chemiczna indukcja; Haploidyzacja; Chemical induction; Haploidization

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Piosik, �. (2014). Obcy gatunkowo pyłek i czynnik chemiczny w indukcji rozwoju zarodków haploidalnych u Lactuca sativa L. (Doctoral Dissertation). Uniwersytet im. Adama Mickiewicza w Poznaniu. Retrieved from http://hdl.handle.net/10593/12207

Chicago Manual of Style (16^th Edition):

Piosik, Łukasz. “Obcy gatunkowo pyłek i czynnik chemiczny w indukcji rozwoju zarodków haploidalnych u Lactuca sativa L. ” 2014. Doctoral Dissertation, Uniwersytet im. Adama Mickiewicza w Poznaniu. Accessed April 17, 2021. http://hdl.handle.net/10593/12207.

MLA Handbook (7^th Edition):

Piosik, Łukasz. “Obcy gatunkowo pyłek i czynnik chemiczny w indukcji rozwoju zarodków haploidalnych u Lactuca sativa L. ” 2014. Web. 17 Apr 2021.

Vancouver:

Piosik �. Obcy gatunkowo pyłek i czynnik chemiczny w indukcji rozwoju zarodków haploidalnych u Lactuca sativa L. [Internet] [Doctoral dissertation]. Uniwersytet im. Adama Mickiewicza w Poznaniu; 2014. [cited 2021 Apr 17]. Available from: http://hdl.handle.net/10593/12207.

Council of Science Editors:

Piosik �. Obcy gatunkowo pyłek i czynnik chemiczny w indukcji rozwoju zarodków haploidalnych u Lactuca sativa L. [Doctoral Dissertation]. Uniwersytet im. Adama Mickiewicza w Poznaniu; 2014. Available from: http://hdl.handle.net/10593/12207

19. Rey, Olivier. Systèmes de reproduction et scénarios d’invasion chez la petite fourmi de feu, Wasmannia auropunctata : Breeding system and scenario of invasion in the little fire ant, Wasmannia auropunctata.

Degree: Docteur es, Biologie des organismes et des populations, 2011, Montpellier, SupAgro

URL: http://www.theses.fr/2011NSAM0046

►

Cette thèse vise à améliorer notre connaissance des processus évolutifs et écologiques liés aux invasions biologiques au travers de l'étude de populations envahissantes et non… (more)

▼

Cette thèse vise à améliorer notre connaissance des processus évolutifs et écologiques liés aux invasions biologiques au travers de l'étude de populations envahissantes et non envahissantes de la petite fourmi de feu, Wasmannia auropunctata. Cette espèce présente un polymorphisme du système de reproduction original. Dans les populations ancestrales, les reines et les mâles se reproduisent selon le mode de reproduction sexué classique des hyménoptères (haplo-diploïde). Dans d'autres populations, les reines sont parthénogénétiques et les mâles sont produits de manière clonale via les œufs pondus par la reine. Ces reines et ces mâles produisent néanmoins des ouvrières stériles sexuellement. Ce mode de reproduction clonal semble associé indirectement au succès d'invasion des populations. Dans un premier temps nous avons identifié les mécanismes sous-jacents au système de reproduction des populations clonales. Nos résultats montrent que les reines utilisent la parthénogenèse automictique associée à une réduction du taux de recombinaison pour la production de reines, l'androgenèse pour la production des mâles et la reproduction sexuée pour la production d'ouvrières stériles. La fixation des génomes parentaux dans les descendances successives permet la reproduction entre individus d'une même cohorte en évitant la dépression de consanguinité dans la descendance ouvrière. Nous avons ensuite montré que le changement de système de reproduction de la sexualité vers la clonalité est associé à un changement adaptatif permettant aux ouvrières des populations clonales de mieux tolérer les températures stressantes caractéristiques des localités envahies, comparativement aux ouvrières des populations sexuées ancestrales. Enfin, l'utilisation d'une approche multidisciplinaire couplant des modèles de distribution d'espèces, des analyses de génétique des populations et des expériences en laboratoire, nous a permis de montrer que les changements évolutifs clefs associés au succès d'invasion des populations, ont lieu dans des habitats marginaux de l'aire native, avant la dispersion vers des localités distantes caractérisées par des conditions environnementales similaires.

The main goal of this thesis is to provide new insights on the evolutionary processes associated to biological invasions through the study of invasive and non-invasive populations of the little fire ant, W. auropunctata. This species is characterised by an eccentric breeding system polymorphism. In ancestral populations, queens and males reproduce following the classical sexual reproduction system of hymenopteran species (haplo-diploid). In some other populations, queens reproduce by parthenogenesis and the males are reproduced clonally through queens' eggs. These clonal queens and males nevertheless produce sterile workers sexually. Interestingly this clonal reproduction seems indirectly associated with the invasive success of populations. In this study, we first identified the mechanisms underlying the breeding system of clonal populations. Our results indicate that…

Advisors/Committee Members: Estoup, Arnaud (thesis director).

Subjects/Keywords: Wasmannia auropunctata; Invasion; Parthénogenèse; Androgenèse; Evolution; Adaptation; Wasmannia auropunctata; Invasion; Parthenogenesis; Androgenesis; Evolution; Adaptation

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Rey, O. (2011). Systèmes de reproduction et scénarios d’invasion chez la petite fourmi de feu, Wasmannia auropunctata : Breeding system and scenario of invasion in the little fire ant, Wasmannia auropunctata. (Doctoral Dissertation). Montpellier, SupAgro. Retrieved from http://www.theses.fr/2011NSAM0046

Chicago Manual of Style (16^th Edition):

Rey, Olivier. “Systèmes de reproduction et scénarios d’invasion chez la petite fourmi de feu, Wasmannia auropunctata : Breeding system and scenario of invasion in the little fire ant, Wasmannia auropunctata.” 2011. Doctoral Dissertation, Montpellier, SupAgro. Accessed April 17, 2021. http://www.theses.fr/2011NSAM0046.

MLA Handbook (7^th Edition):

Rey, Olivier. “Systèmes de reproduction et scénarios d’invasion chez la petite fourmi de feu, Wasmannia auropunctata : Breeding system and scenario of invasion in the little fire ant, Wasmannia auropunctata.” 2011. Web. 17 Apr 2021.

Vancouver:

Rey O. Systèmes de reproduction et scénarios d’invasion chez la petite fourmi de feu, Wasmannia auropunctata : Breeding system and scenario of invasion in the little fire ant, Wasmannia auropunctata. [Internet] [Doctoral dissertation]. Montpellier, SupAgro; 2011. [cited 2021 Apr 17]. Available from: http://www.theses.fr/2011NSAM0046.

Council of Science Editors:

Rey O. Systèmes de reproduction et scénarios d’invasion chez la petite fourmi de feu, Wasmannia auropunctata : Breeding system and scenario of invasion in the little fire ant, Wasmannia auropunctata. [Doctoral Dissertation]. Montpellier, SupAgro; 2011. Available from: http://www.theses.fr/2011NSAM0046

20. Miyauchi, Tochimara Aparecida. Aspectos biológicos do desenvolvimento pré- implantacional de embriões bovinos partenogenéticos ou fecundados in vitro.

Degree: 2012, Universidade Jose do Rosario Vellano; Programa de Mestrado em Medicina Veterinária; UNIFENAS; BR; Reprodução Animal

URL: http://tede2.unifenas.br:8080/jspui/handle/jspui/140

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Made available in DSpace on 2016-05-02T13:55:47Z (GMT). No. of bitstreams: 1 TochimaraAparecidaMiyauchi-dissertacao.pdf: 1277501 bytes, checksum: 28f321c050df674dd0101fe282f265af (MD5) Previous issue date: 2012-09-28

Coordenacao de Aperfeicoamento de… (more)

▼

Made available in DSpace on 2016-05-02T13:55:47Z (GMT). No. of bitstreams: 1 TochimaraAparecidaMiyauchi-dissertacao.pdf: 1277501 bytes, checksum: 28f321c050df674dd0101fe282f265af (MD5) Previous issue date: 2012-09-28

Coordenacao de Aperfeicoamento de Pessoal de Nïvel Superior

Parthenogenesis has been described as an alternative method to produce embryos for studies with embryonic cells, particularly in humans which have a restriction of fertilized embryos. Procedures used in parthenogenesis are also required to produce embryos by somatic cell nuclear transfer in domestic species. However, many biological, cellular and molecular aspects of parthenogenetic embryos are still unknown. This study aimed to compare the kinetics of development, apoptosis rate and gene expression of stress and celular metabolism in bovine parthenogenetic embryos and embryos fertilized in vitro. Oocytes (n = 1541) obtained from slaughterhouse ovaries were matured in vitro and submitted to parthenogenetic activation (4.62 µM ionomycin for 5 min followed by 2 mM 6-DMAP for 4 h) or in vitro fertilization (2 x 106 sperm/mL for 20h with semen from a single departure). 72h postactivation/fertilization (hpaf), embryos (8-cell) were frozen for subsequent analysis of gene expression and another part has been separated into groups of high or low potential of development: Part ≥ 8 - parthenogenetic embryos with 8 or more cells (high development potential); Part < 8 - embryos less than 8 cells (low development potential); ≥ 8 IVF - in vitro fertilized embryos with 8 or more cells; and IVF < 8 - embryos with less than 8 cells. Embryos were cultured in CR2aa medium with 2.5% BFS in 5% CO2, 5% O2, 90% N2 at 38.5° C and were evaluated rates of blastocyst 168hpaf (D7) and 196hpaf (D8). 8-cell embryos obtained after 72hpfa were analyzed for gene expression. D8 blastocysts were fixed and subsequently apoptotic index was analyzed by TUNEL. Data were compared by analysis of variance and means by Student Newman Keuls test. Gene expression was evaluated by REST® software. Values are shown as mean ± standard error. Embryos with 8 or more cells produced higher (P < 0.01) blastocysts rates in D7 and D8 compared to embryos with less than eight cells, showing its greatest development potential, regardless if they were parthenogenetic or fertilized. Embryos of Part ≥ 8 group had higher (P <0.05) blastocyst rate in D7 (63.6 ± 3.4%) than IVF ≥ 8 (45.3 ± 8.9%), but at D8 rate was similar (56.7 ± 3.0% and 44.2 ± 8.9% for Part ≥ 8 and IVF ≥ 8, respectively; P <0.05). There was no difference (P < 0.05) on blastocyst rates at D7 and D8 between embryos with less than 8 cells derived from parthenogenesis or fertilization. There was no difference in total cell number (92.0 ± 3.4, 102.30 ± 4.8), apoptotic cells (10.8 ± 1.22, 9.5 ± 1.07) and apoptotic index (11.4 ± 1.27, 9.8 ± 1.8) for parthenogenetic and IVF blastocysts, respectively. In 8-cell embryos analyzed, there was a subexpression of genes DNAJB1, HSPA1L, HSF1 and GLUT1 for Part in…

Advisors/Committee Members: Camargo, Luiz Sérgio de Almeida, Fernandes, Carlos Antônio de Carvalho, Arashiro, Eduardo Kenji Nunes.

Subjects/Keywords: partenogênese; expressão gênica; embrião; bovino; parthenogenesis; gene expression; embryo; bovine; CNPQ::CIENCIAS AGRARIAS::MEDICINA VETERINARIA

Record Details Similar Records Cite « Share

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Miyauchi, T. A. (2012). Aspectos biológicos do desenvolvimento pré- implantacional de embriões bovinos partenogenéticos ou fecundados in vitro. (Masters Thesis). Universidade Jose do Rosario Vellano; Programa de Mestrado em Medicina Veterinária; UNIFENAS; BR; Reprodução Animal. Retrieved from http://tede2.unifenas.br:8080/jspui/handle/jspui/140

Chicago Manual of Style (16^th Edition):

Miyauchi, Tochimara Aparecida. “Aspectos biológicos do desenvolvimento pré- implantacional de embriões bovinos partenogenéticos ou fecundados in vitro.” 2012. Masters Thesis, Universidade Jose do Rosario Vellano; Programa de Mestrado em Medicina Veterinária; UNIFENAS; BR; Reprodução Animal. Accessed April 17, 2021. http://tede2.unifenas.br:8080/jspui/handle/jspui/140.

MLA Handbook (7^th Edition):

Miyauchi, Tochimara Aparecida. “Aspectos biológicos do desenvolvimento pré- implantacional de embriões bovinos partenogenéticos ou fecundados in vitro.” 2012. Web. 17 Apr 2021.

Vancouver:

Miyauchi TA. Aspectos biológicos do desenvolvimento pré- implantacional de embriões bovinos partenogenéticos ou fecundados in vitro. [Internet] [Masters thesis]. Universidade Jose do Rosario Vellano; Programa de Mestrado em Medicina Veterinária; UNIFENAS; BR; Reprodução Animal; 2012. [cited 2021 Apr 17]. Available from: http://tede2.unifenas.br:8080/jspui/handle/jspui/140.

Council of Science Editors:

Miyauchi TA. Aspectos biológicos do desenvolvimento pré- implantacional de embriões bovinos partenogenéticos ou fecundados in vitro. [Masters Thesis]. Universidade Jose do Rosario Vellano; Programa de Mestrado em Medicina Veterinária; UNIFENAS; BR; Reprodução Animal; 2012. Available from: http://tede2.unifenas.br:8080/jspui/handle/jspui/140

21. Miyauchi, Tochimara Aparecida. Aspectos biológicos do desenvolvimento pré- implantacional de embriões bovinos partenogenéticos ou fecundados in vitro.

Degree: 2012, Universidade Jose do Rosario Vellano; Programa de Mestrado em Medicina Veterinária; UNIFENAS; BR; Reprodução Animal

URL: http://tede2.unifenas.br:8080/jspui/handle/jspui/141

►

Made available in DSpace on 2016-05-02T13:55:48Z (GMT). No. of bitstreams: 1 Tochimara Aparecida Myauchi Dissertacao.pdf: 1469858 bytes, checksum: d9554237da81a72762ef7629c0dfa1b8 (MD5) Previous issue date: 2012-09-28

Coordenacao… (more)

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Made available in DSpace on 2016-05-02T13:55:48Z (GMT). No. of bitstreams: 1 Tochimara Aparecida Myauchi Dissertacao.pdf: 1469858 bytes, checksum: d9554237da81a72762ef7629c0dfa1b8 (MD5) Previous issue date: 2012-09-28

Coordenacao de Aperfeicoamento de Pessoal de Nïvel Superior

Parthenogenesis has been described as an alternative method to produce embryos for studies with embryonic cells, particularly in humans which have a restriction of fertilized embryos. Procedures used in parthenogenesis are also required to produce embryos by somatic cell nuclear transfer in domestic species. However, many biological, cellular and molecular aspects of parthenogenetic embryos are still unknown. This study aimed to compare the kinetics of development, apoptosis rate and gene expression of stress and celular metabolism in bovine parthenogenetic embryos and embryos fertilized in vitro. Oocytes (n = 1541) obtained from slaughterhouse ovaries were matured in vitro and submitted to parthenogenetic activation (4.62 µM ionomycin for 5 min followed by 2 mM 6-DMAP for 4 h) or in vitro fertilization (2 x 106 sperm/mL for 20h with semen from a single departure). 72h postactivation/fertilization (hpaf), embryos (8-cell) were frozen for subsequent analysis of gene expression and another part has been separated into groups of high or low potential of development: Part ≥ 8 - parthenogenetic embryos with 8 or more cells (high development potential); Part < 8 - embryos less than 8 cells (low development potential); ≥ 8 IVF - in vitro fertilized embryos with 8 or more cells; and IVF < 8 - embryos with less than 8 cells. Embryos were cultured in CR2aa medium with 2.5% BFS in 5% CO2, 5% O2, 90% N2 at 38.5° C and were evaluated rates of blastocyst 168hpaf (D7) and 196hpaf (D8). 8-cell embryos obtained after 72hpfa were analyzed for gene expression. D8 blastocysts were fixed and subsequently apoptotic index was analyzed by TUNEL. Data were compared by analysis of variance and means by Student Newman Keuls test. Gene expression was evaluated by REST® software. Values are shown as mean ± standard error. Embryos with 8 or more cells produced higher (P < 0.01) blastocysts rates in D7 and D8 compared to embryos with less than eight cells, showing its greatest development potential, regardless if they were parthenogenetic or fertilized. Embryos of Part ≥ 8 group had higher (P <0.05) blastocyst rate in D7 (63.6 ± 3.4%) than IVF ≥ 8 (45.3 ± 8.9%), but at D8 rate was similar (56.7 ± 3.0% and 44.2 ± 8.9% for Part ≥ 8 and IVF ≥ 8, respectively; P <0.05). There was no difference (P < 0.05) on blastocyst rates at D7 and D8 between embryos with less than 8 cells derived from parthenogenesis or fertilization. There was no difference in total cell number (92.0 ± 3.4, 102.30 ± 4.8), apoptotic cells (10.8 ± 1.22, 9.5 ± 1.07) and apoptotic index (11.4 ± 1.27, 9.8 ± 1.8) for parthenogenetic and IVF blastocysts, respectively. In 8-cell embryos analyzed, there was a subexpression of genes DNAJB1, HSPA1L, HSF1 and GLUT1 for Part in…

Advisors/Committee Members: Camargo, Luiz Sérgio de Almeida, Fernandes, Carlos Antônio de Carvalho, Arashiro, Eduardo Kenji Nunes.

Subjects/Keywords: partenogênese; expressão gênica; embrião; bovino; parthenogenesis; gene expression.; embryo; bovine; CNPQ::CIENCIAS AGRARIAS::MEDICINA VETERINARIA

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Miyauchi, T. A. (2012). Aspectos biológicos do desenvolvimento pré- implantacional de embriões bovinos partenogenéticos ou fecundados in vitro. (Masters Thesis). Universidade Jose do Rosario Vellano; Programa de Mestrado em Medicina Veterinária; UNIFENAS; BR; Reprodução Animal. Retrieved from http://tede2.unifenas.br:8080/jspui/handle/jspui/141

Chicago Manual of Style (16^th Edition):

Miyauchi, Tochimara Aparecida. “Aspectos biológicos do desenvolvimento pré- implantacional de embriões bovinos partenogenéticos ou fecundados in vitro.” 2012. Masters Thesis, Universidade Jose do Rosario Vellano; Programa de Mestrado em Medicina Veterinária; UNIFENAS; BR; Reprodução Animal. Accessed April 17, 2021. http://tede2.unifenas.br:8080/jspui/handle/jspui/141.

MLA Handbook (7^th Edition):

Miyauchi, Tochimara Aparecida. “Aspectos biológicos do desenvolvimento pré- implantacional de embriões bovinos partenogenéticos ou fecundados in vitro.” 2012. Web. 17 Apr 2021.

Vancouver:

Miyauchi TA. Aspectos biológicos do desenvolvimento pré- implantacional de embriões bovinos partenogenéticos ou fecundados in vitro. [Internet] [Masters thesis]. Universidade Jose do Rosario Vellano; Programa de Mestrado em Medicina Veterinária; UNIFENAS; BR; Reprodução Animal; 2012. [cited 2021 Apr 17]. Available from: http://tede2.unifenas.br:8080/jspui/handle/jspui/141.

Council of Science Editors:

Miyauchi TA. Aspectos biológicos do desenvolvimento pré- implantacional de embriões bovinos partenogenéticos ou fecundados in vitro. [Masters Thesis]. Universidade Jose do Rosario Vellano; Programa de Mestrado em Medicina Veterinária; UNIFENAS; BR; Reprodução Animal; 2012. Available from: http://tede2.unifenas.br:8080/jspui/handle/jspui/141

22. Miyauchi, Tochimara Aparecida. Aspectos biológicos do desenvolvimento pré- implantacional de embriões bovinos partenogenéticos ou fecundados in vitro.

Degree: 2012, Universidade Jose do Rosario Vellano; Programa de Mestrado em Medicina Veterinária; UNIFENAS; BR; Reprodução Animal

URL: http://tede2.unifenas.br:8080/jspui/handle/jspui/149

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Made available in DSpace on 2016-05-02T13:55:51Z (GMT). No. of bitstreams: 1 TochimaraAparecidaMiyauchi-dissertacao.pdf: 849651 bytes, checksum: ef29d9bc544c7cef9bb8b27b98b198ea (MD5) Previous issue date: 2012-09-28

Coordenacao de Aperfeicoamento de… (more)

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Made available in DSpace on 2016-05-02T13:55:51Z (GMT). No. of bitstreams: 1 TochimaraAparecidaMiyauchi-dissertacao.pdf: 849651 bytes, checksum: ef29d9bc544c7cef9bb8b27b98b198ea (MD5) Previous issue date: 2012-09-28

Coordenacao de Aperfeicoamento de Pessoal de Nïvel Superior

Parthenogenesis has been described as an alternative method to produce embryos for studies with embryonic cells, particularly in humans which have a restriction of fertilized embryos. Procedures used in parthenogenesis are also required to produce embryos by somatic cell nuclear transfer in domestic species. However, many biological, cellular and molecular aspects of parthenogenetic embryos are still unknown. This study aimed to compare the kinetics of development, apoptosis rate and gene expression of stress and celular metabolism in bovine parthenogenetic embryos and embryos fertilized in vitro. Oocytes (n = 1541) obtained from slaughterhouse ovaries were matured in vitro and submitted to parthenogenetic activation (4.62 µM ionomycin for 5 min followed by 2 mM 6-DMAP for 4 h) or in vitro fertilization (2 x 106 sperm/mL for 20h with semen from a single departure). 72h postactivation/fertilization (hpaf), embryos (8-cell) were frozen for subsequent analysis of gene expression and another part has been separated into groups of high or low potential of development: Part ≥ 8 - parthenogenetic embryos with 8 or more cells (high development potential); Part < 8 - embryos less than 8 cells (low development potential); ≥ 8 IVF - in vitro fertilized embryos with 8 or more cells; and IVF < 8 - embryos with less than 8 cells. Embryos were cultured in CR2aa medium with 2.5% BFS in 5% CO2, 5% O2, 90% N2 at 38.5° C and were evaluated rates of blastocyst 168hpaf (D7) and 196hpaf (D8). 8-cell embryos obtained after 72hpfa were analyzed for gene expression. D8 blastocysts were fixed and subsequently apoptotic index was analyzed by TUNEL. Data were compared by analysis of variance and means by Student Newman Keuls test. Gene expression was evaluated by REST® software. Values are shown as mean ± standard error. Embryos with 8 or more cells produced higher (P < 0.01) blastocysts rates in D7 and D8 compared to embryos with less than eight cells, showing its greatest development potential, regardless if they were parthenogenetic or fertilized. Embryos of Part ≥ 8 group had higher (P <0.05) blastocyst rate in D7 (63.6 ± 3.4%) than IVF ≥ 8 (45.3 ± 8.9%), but at D8 rate was similar (56.7 ± 3.0% and 44.2 ± 8.9% for Part ≥ 8 and IVF ≥ 8, respectively; P <0.05). There was no difference (P < 0.05) on blastocyst rates at D7 and D8 between embryos with less than 8 cells derived from parthenogenesis or fertilization. There was no difference in total cell number (92.0 ± 3.4, 102.30 ± 4.8), apoptotic cells (10.8 ± 1.22, 9.5 ± 1.07) and apoptotic index (11.4 ± 1.27, 9.8 ± 1.8) for parthenogenetic and IVF blastocysts, respectively. In 8-cell embryos analyzed, there was a subexpression of genes DNAJB1, HSPA1L, HSF1 and GLUT1 for Part in…

Advisors/Committee Members: Camargo, Luiz Sérgio de Almeida, Fernandes, Carlos Antônio de Carvalho, Arashiro, Eduardo Kenji Nunes.

Subjects/Keywords: partenogênese; expressão gênica; embrião; bovino; parthenogenesis; gene expression; embryo; bovine; CNPQ::CIENCIAS BIOLOGICAS::MORFOLOGIA::EMBRIOLOGIA

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Miyauchi, T. A. (2012). Aspectos biológicos do desenvolvimento pré- implantacional de embriões bovinos partenogenéticos ou fecundados in vitro. (Masters Thesis). Universidade Jose do Rosario Vellano; Programa de Mestrado em Medicina Veterinária; UNIFENAS; BR; Reprodução Animal. Retrieved from http://tede2.unifenas.br:8080/jspui/handle/jspui/149

Chicago Manual of Style (16^th Edition):

Miyauchi, Tochimara Aparecida. “Aspectos biológicos do desenvolvimento pré- implantacional de embriões bovinos partenogenéticos ou fecundados in vitro.” 2012. Masters Thesis, Universidade Jose do Rosario Vellano; Programa de Mestrado em Medicina Veterinária; UNIFENAS; BR; Reprodução Animal. Accessed April 17, 2021. http://tede2.unifenas.br:8080/jspui/handle/jspui/149.

MLA Handbook (7^th Edition):

Miyauchi, Tochimara Aparecida. “Aspectos biológicos do desenvolvimento pré- implantacional de embriões bovinos partenogenéticos ou fecundados in vitro.” 2012. Web. 17 Apr 2021.

Vancouver:

Miyauchi TA. Aspectos biológicos do desenvolvimento pré- implantacional de embriões bovinos partenogenéticos ou fecundados in vitro. [Internet] [Masters thesis]. Universidade Jose do Rosario Vellano; Programa de Mestrado em Medicina Veterinária; UNIFENAS; BR; Reprodução Animal; 2012. [cited 2021 Apr 17]. Available from: http://tede2.unifenas.br:8080/jspui/handle/jspui/149.

Council of Science Editors:

Miyauchi TA. Aspectos biológicos do desenvolvimento pré- implantacional de embriões bovinos partenogenéticos ou fecundados in vitro. [Masters Thesis]. Universidade Jose do Rosario Vellano; Programa de Mestrado em Medicina Veterinária; UNIFENAS; BR; Reprodução Animal; 2012. Available from: http://tede2.unifenas.br:8080/jspui/handle/jspui/149

University of Alberta

23. Smith, Alexander. Rising from the ashes; an unanticipated failure with the engorgement factor voraxin leads to advances in three areas of tick biology: Developmental abnormalities and parthenogenesis, identification of a Coxiella-like symbiont and the molecular characterization of vitellogenesis in the southern African bont tick, Amblyomma hebraeum Koch (1844).

Degree: PhD, Department of Biological Sciences, 2012, University of Alberta

URL: https://era.library.ualberta.ca/files/2z10wq62x

► Weiss and Kaufman (2004, PNAS 101: 5874-5879) demonstrated that injections of fed male gonad homogenates stimulate engorgement in virgin female Amblyomma hebraeum ticks, due to… (more)

▼ Weiss and Kaufman (2004, PNAS 101: 5874-5879) demonstrated that injections of fed male gonad homogenates stimulate engorgement in virgin female Amblyomma hebraeum ticks, due to the presence of the engorgement factor voraxin. They also showed voraxin's potential as part of an anti-tick vaccine: 74% of mated females fed on a rabbit immunized against voraxin failed to engorge. The original aim of this thesis was to further characterize and better evaluate the potential of voraxin as a component of a novel anti-tick vaccine. However, I was unable to confirm Weiss and Kaufman's results. Injections of male gonad homogenates or recombinant voraxin produced in two different bacterial expression systems into virgin females, both on and off the host, failed to stimulate engorgement. Immunization of rabbits against the recombinant voraxin proteins also failed to inhibit tick feeding. Silencing of voraxin via RNAi was unsuccessful, but I was able to silence subolesin, an important modulator of tick feeding and reproduction. I also observed a large number of morphological abnormalities in our ticks, as well as several virgin females that engorged and laid viable eggs. Although low levels of parthenogenesis have been reported in many other normally bisexual tick species, it has not been previously reported in A. hebraeum. The causes of these abnormalities are unknown, but could be due, in part, to the high degree of inbreeding in our lab colony. In addition to the numerous bacterial pathogens transmitted by ticks, many tick species also harbour a number of potentially mutualistic endosymbiotic bacteria. Here I have determined that out tick colony possess both Coxiella-like and Rickettsia-like symbionts. Their impact on the biology of the tick is unknown. Although much is known about vitellogenesis and its regulation in insects, our knowledge in this area is much more limited in ticks. I have sequenced the full-length cDNAs encoding two vitellogenins and the vitellogenin receptor from A. hebraeum, which are similar to those of other arthropods. RT-PCR analysis of gene expression showed that the vitellogenin receptor is expressed only in the ovary of fed females, whereas vitellogenin is produced in the fat body and midgut.

Subjects/Keywords: engorgement factor; ticks; vitellogenin; Ixodida; reproduction; Amblyomma hebraeum; morphological abnormalities; morphology; vitellogenesis; vitellogenin receptor; Acari; parthenogenesis; voraxin

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Smith, A. (2012). Rising from the ashes; an unanticipated failure with the engorgement factor voraxin leads to advances in three areas of tick biology: Developmental abnormalities and parthenogenesis, identification of a Coxiella-like symbiont and the molecular characterization of vitellogenesis in the southern African bont tick, Amblyomma hebraeum Koch (1844). (Doctoral Dissertation). University of Alberta. Retrieved from https://era.library.ualberta.ca/files/2z10wq62x

Chicago Manual of Style (16^th Edition):

Smith, Alexander. “Rising from the ashes; an unanticipated failure with the engorgement factor voraxin leads to advances in three areas of tick biology: Developmental abnormalities and parthenogenesis, identification of a Coxiella-like symbiont and the molecular characterization of vitellogenesis in the southern African bont tick, Amblyomma hebraeum Koch (1844).” 2012. Doctoral Dissertation, University of Alberta. Accessed April 17, 2021. https://era.library.ualberta.ca/files/2z10wq62x.

MLA Handbook (7^th Edition):

Smith, Alexander. “Rising from the ashes; an unanticipated failure with the engorgement factor voraxin leads to advances in three areas of tick biology: Developmental abnormalities and parthenogenesis, identification of a Coxiella-like symbiont and the molecular characterization of vitellogenesis in the southern African bont tick, Amblyomma hebraeum Koch (1844).” 2012. Web. 17 Apr 2021.

Vancouver:

Smith A. Rising from the ashes; an unanticipated failure with the engorgement factor voraxin leads to advances in three areas of tick biology: Developmental abnormalities and parthenogenesis, identification of a Coxiella-like symbiont and the molecular characterization of vitellogenesis in the southern African bont tick, Amblyomma hebraeum Koch (1844). [Internet] [Doctoral dissertation]. University of Alberta; 2012. [cited 2021 Apr 17]. Available from: https://era.library.ualberta.ca/files/2z10wq62x.

Council of Science Editors:

Smith A. Rising from the ashes; an unanticipated failure with the engorgement factor voraxin leads to advances in three areas of tick biology: Developmental abnormalities and parthenogenesis, identification of a Coxiella-like symbiont and the molecular characterization of vitellogenesis in the southern African bont tick, Amblyomma hebraeum Koch (1844). [Doctoral Dissertation]. University of Alberta; 2012. Available from: https://era.library.ualberta.ca/files/2z10wq62x

24. Joana Claudia Mezzalira. Efeito da heteroplasmia na densidade celular e desenvolvimento embrionÃrio in vitro de embriÃes bovinos clonados por transferÃncia nuclear de cÃlula somÃtica.

Degree: 2009, Universidade do Estado de Santa Catarina

URL: http://www.tede.udesc.br/tde_busca/arquivo.php?codArquivo=3548

► Na clonagem por transferÃncia nuclear com cÃlula somÃtica (TNCS), o tipo de citoplasto receptor desempenha papel chave na reprogramaÃÃo nuclear. Distintos citoplastos e carioplastos e… (more)

▼ Na clonagem por transferÃncia nuclear com cÃlula somÃtica (TNCS), o tipo de citoplasto receptor desempenha papel chave na reprogramaÃÃo nuclear. Distintos citoplastos e carioplastos e condiÃÃes de ativaÃÃo foram utilizadas na reconstruÃÃo de embriÃes bovinos com o objetivo de avaliar o efeito do tipo de citoplasto (oÃcito e/ou zigoto) e do mÃtodo de ativaÃÃo (quÃmica, AQ, ou espermÃtica, AE) no desenvolvimento de blastocistos clonados produzidos pela tÃcnica de clonagem manual (Handmade Cloning, HMC). ApÃs 17 h de maturaÃÃo in vitro (MIV), 2.946 oÃcitos foram enucleados por bissecÃÃo manual, resultando em hemi-oÃcitos enucleados (citoplastos MII) e nÃo enucleados (carioplastos MII). Outros 2.368 oÃcitos submetidos a 6 h de fecundaÃÃo in vitro (FIV) foram bisseccionados manualmente e segregados em hemi-zigotos enucleados (citoplastos FIV) e nÃo enucleados (carioplastos FIV). CÃlulas de um cultivo celular estabelecido a partir da biÃpsia auricular de uma fÃmea bovina adulta foram utilizadas como nÃcleos doadores (carioplasto CS). As estruturas foram dispostas em (a) grupos controle: FIV; partenogÃnese com oÃcitos com (PG c/) ou sem zona pelÃcida (PG s/); e clone por TNCS; ou (b) grupos experimentais: G1, citoplasto FIV + citoplasto MII + carioplasto CS; G2, citoplasto MII + carioplasto FIV; G3, citoplasto FIV + carioplasto FIV; G4, citoplasto FIV + citoplasto FIV + carioplasto CS; e G5, citoplasto MII + carioplasto MII. ApÃs a eletrofusÃo das estruturas, os grupos experimentais G1 a G45 foram divididos em subgrupos de AQ ou AE. O cultivo in vitro foi realizado pelo sistema WOW (well-of-the-well). ApÃs 20 repetiÃÃes, as taxas de clivagem (D2) e blastocisto (D7) foram comparadas pelos testes de χ2 e os valores para o nÃmero total de cÃlulas e a alocaÃÃo das linhagens celulares nos blastocistos, determinados por coloraÃÃo diferencial, foram avaliados por anÃlise de variÃncia, com pareamento comparativo pelo teste de Tukey, para P<0,05. O Ãnico grupo experimental que apresentou desenvolvimento embrionÃrio no D7 semelhante aos controles FIV (27,0%) e TNCS (31,4%) foi o subgrupo G1 AE (28,2%). Isso pode ser atribuÃdo a uma melhor sincronia do ciclo celular entre citoplastos e/ou carioplasto e um mais adequado processo de ativaÃÃo. O desenvolvimento embrionÃrio nos grupos G1 AQ (13,7%), G4 AQ (6,4%) e G4 AE (8,7%) foi menor do que o G1 AE, possivelmente devido Ã assincronia do processo de ativaÃÃo ou ciclo celular. O desenvolvimento embrionÃrio nulo dos grupos G2 e G3, independente da ativaÃÃo, possivelmente foi decorrente da manipulaÃÃo das estruturas em um momento biologicamente sensÃvel. Da mesma forma, a baixa clivagem (57,0%) e o desenvolvimento nulo no grupo G5 de ativaÃÃo espontÃnea demonstraram de fato que a manipulaÃÃo estimulou o processo de ativaÃÃo embrionÃria de forma sub-limiar. Em geral, nÃo houve diferenÃa no nÃmero de cÃlulas e alocaÃÃo celular nos grupos onde houve desenvolvimento atÃ o estÃdio de blastocisto. Conclui-se que o processo de ativaÃÃo foi tÃo significativo para o desenvolvimento embrionÃrio… Advisors/Committee Members: Marcelo Bertolini.

Subjects/Keywords: SCNT; handmade cloning; partenogÃnese; bovinos; ciclo celular; ativaÃÃo embrionÃria; SCNT; handmade cloning; parthenogenesis; cattle; cell cycle; embryo activation; MEDICINA VETERINARIA

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Mezzalira, J. C. (2009). Efeito da heteroplasmia na densidade celular e desenvolvimento embrionÃrio in vitro de embriÃes bovinos clonados por transferÃncia nuclear de cÃlula somÃtica. (Thesis). Universidade do Estado de Santa Catarina. Retrieved from http://www.tede.udesc.br/tde_busca/arquivo.php?codArquivo=3548

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Mezzalira, Joana Claudia. “Efeito da heteroplasmia na densidade celular e desenvolvimento embrionÃrio in vitro de embriÃes bovinos clonados por transferÃncia nuclear de cÃlula somÃtica.” 2009. Thesis, Universidade do Estado de Santa Catarina. Accessed April 17, 2021. http://www.tede.udesc.br/tde_busca/arquivo.php?codArquivo=3548.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Mezzalira, Joana Claudia. “Efeito da heteroplasmia na densidade celular e desenvolvimento embrionÃrio in vitro de embriÃes bovinos clonados por transferÃncia nuclear de cÃlula somÃtica.” 2009. Web. 17 Apr 2021.

Vancouver:

Mezzalira JC. Efeito da heteroplasmia na densidade celular e desenvolvimento embrionÃrio in vitro de embriÃes bovinos clonados por transferÃncia nuclear de cÃlula somÃtica. [Internet] [Thesis]. Universidade do Estado de Santa Catarina; 2009. [cited 2021 Apr 17]. Available from: http://www.tede.udesc.br/tde_busca/arquivo.php?codArquivo=3548.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Mezzalira JC. Efeito da heteroplasmia na densidade celular e desenvolvimento embrionÃrio in vitro de embriÃes bovinos clonados por transferÃncia nuclear de cÃlula somÃtica. [Thesis]. Universidade do Estado de Santa Catarina; 2009. Available from: http://www.tede.udesc.br/tde_busca/arquivo.php?codArquivo=3548

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Texas A&M University

25. Dickey, Aaron. Host-Associated Differentiation in an Insect Community.

Degree: PhD, Entomology, 2011, Texas A&M University

URL: http://hdl.handle.net/1969.1/ETD-TAMU-2010-12-8695

► Host-Associated Differentiation (HAD) is the formation of genetically divergent hostassociated lineages maintained by ecological isolation. HAD is potentially an important route to ecological speciation in… (more)

▼ Host-Associated Differentiation (HAD) is the formation of genetically divergent hostassociated lineages maintained by ecological isolation. HAD is potentially an important route to ecological speciation in parasites including many insects. While HAD case studies are accumulating, there is a dearth of negative results in the literature making it difficult to know how common the phenomenon really is or whether there are specific traits of parasites which promote HAD. To address these two problems, studies are needed which both publish negative results (i.e., parasites not showing HAD) and test for HAD in multiple parasite species on the same pair of host species (i.e., control for host plant effects). In this study, HAD was tested in three species of herbivorous insects and one parasitoid species on the same two host tree species: pecan and water hickory. The insects were selected based on the presence or absence of two traits, parthenogenesis and endophagy. A test for HAD was considered “positive” when population substructure was explained by host-association. To test for the presence of HAD, insects were sampled sympatrically to eliminate geographical isolation as a confounding factor, sampling was replicated spatially to assure that HAD persisted, and multiple loci were sampled from each individual. Genetic data was analyzed using cluster analyses. HAD was found in both pecan leaf phylloxera and yellow pecan aphid but not in pecan bud moth or in the parasitoid of the yellow pecan aphid, Aphelinus perpallidus. Interestingly, both taxa showing HAD are parthenogenetic and both taxa not showing HAD reproduce sexually. Species showing HAD were tested for the presence of a pre-mating reproductive isolating mechanism (RIM) which could be maintaining HAD despite the potential for gene flow. Selection against migrants to the alternative host was tested in yellow pecan aphid using a no-choice fitness experiment. The overall contribution of this RIM to total isolation was positive and ranged from 0.614 to 0.850. The RIM of “habitat preference” was tested in pecan leaf phylloxera using a dual-choice preference experiment. In this species, preference was only detected for phylloxera originating from water hickory suggesting that host discrimination ability may be a less important factor promoting differentiation in phylloxera. Advisors/Committee Members: Medina, Raul F. (advisor), Eubanks, Micky D. (committee member), Harris, Marvin K. (committee member), DeWitt, Thomas J. (committee member).

Subjects/Keywords: Parthenogenesis; Endophagy; Aphids; Carya, Solidago, Genetic Differentiation; Amplified Fragment Length Polymorphisms; Host-Parasite Interactions; Reciprocal Transplant; Host Plant Discrimination; Sequential Radiation

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Dickey, A. (2011). Host-Associated Differentiation in an Insect Community. (Doctoral Dissertation). Texas A&M University. Retrieved from http://hdl.handle.net/1969.1/ETD-TAMU-2010-12-8695

Chicago Manual of Style (16^th Edition):

Dickey, Aaron. “Host-Associated Differentiation in an Insect Community.” 2011. Doctoral Dissertation, Texas A&M University. Accessed April 17, 2021. http://hdl.handle.net/1969.1/ETD-TAMU-2010-12-8695.

MLA Handbook (7^th Edition):

Dickey, Aaron. “Host-Associated Differentiation in an Insect Community.” 2011. Web. 17 Apr 2021.

Vancouver:

Dickey A. Host-Associated Differentiation in an Insect Community. [Internet] [Doctoral dissertation]. Texas A&M University; 2011. [cited 2021 Apr 17]. Available from: http://hdl.handle.net/1969.1/ETD-TAMU-2010-12-8695.

Council of Science Editors:

Dickey A. Host-Associated Differentiation in an Insect Community. [Doctoral Dissertation]. Texas A&M University; 2011. Available from: http://hdl.handle.net/1969.1/ETD-TAMU-2010-12-8695

26. Vollmer, Alicia A. Rare Parthenogenic Reproduction in a Common Reef Coral, Porites astreoides.

Degree: 2018, Nova Southeastern University

URL: https://nsuworks.nova.edu/occ_stuetd/464

► Multiple stressors have caused a decline in coral populations. Broadcast spawning corals once dominated the Florida Reef Tract (FRT), but since their decline, smaller brooding… (more)

Subjects/Keywords: Porites astreoides; parthenogenesis; planulation; density dependence; asexual reproduction; Marine Biology; Oceanography and Atmospheric Sciences and Meteorology

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Vollmer, A. A. (2018). Rare Parthenogenic Reproduction in a Common Reef Coral, Porites astreoides. (Thesis). Nova Southeastern University. Retrieved from https://nsuworks.nova.edu/occ_stuetd/464

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Vollmer, Alicia A. “Rare Parthenogenic Reproduction in a Common Reef Coral, Porites astreoides.” 2018. Thesis, Nova Southeastern University. Accessed April 17, 2021. https://nsuworks.nova.edu/occ_stuetd/464.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Vollmer, Alicia A. “Rare Parthenogenic Reproduction in a Common Reef Coral, Porites astreoides.” 2018. Web. 17 Apr 2021.

Vancouver:

Vollmer AA. Rare Parthenogenic Reproduction in a Common Reef Coral, Porites astreoides. [Internet] [Thesis]. Nova Southeastern University; 2018. [cited 2021 Apr 17]. Available from: https://nsuworks.nova.edu/occ_stuetd/464.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Vollmer AA. Rare Parthenogenic Reproduction in a Common Reef Coral, Porites astreoides. [Thesis]. Nova Southeastern University; 2018. Available from: https://nsuworks.nova.edu/occ_stuetd/464

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Georgia

27. Zeng, Yajuan. Identification and characterization of apospory candidate genes in Pennisetum and Cenchrus.

Degree: 2014, University of Georgia

URL: http://hdl.handle.net/10724/26204

► Apomixis, asexual seed reproduction by avoidance of meiosis and fertilization of the egg cell, holds great potential for agriculture as a means to fix hybrid… (more)

▼ Apomixis, asexual seed reproduction by avoidance of meiosis and fertilization of the egg cell, holds great potential for agriculture as a means to fix hybrid vigor since offspring generated through apomixis are genetic clones of the maternal plant. Apospory is a form of apomixis where the embryo develops from an unreduced egg that is derived from the aposporous embryo sac. In both Pennisetum squamulatum and Cenchrus ciliaris, apospory inherits as a dominant trait under genetic control of a single locus and transmits with an apospory-specific genomic region (ASGR). In order to understand the molecular mechanism regulating apospory, two major studies have been conducted. Aposporous initial specification is a critical event for the occurrence of apospory. To elucidate the mechanism controlling this process, two transcriptomes, derived from ovules of an apomictic donor parent and its apomictic backcross derivative at the stage of apospory initiation, were sequenced using 454-FLX technology. Analysis of the two transcriptomes allowed identification of the ASGR-carrier chromosome linked transcripts. Moreover, one of these alien expressed genes was assigned to the ASGR by screening a limited number of apomictic and sexual F1s. Identification of potential ASGR-linked candidate genes will provide significant insight into the regulation of apospory initiation. The results suggest that the strategy of comparative sequencing of donor parent and backcross transcriptomes is an efficient approach to identify alien expressed genes in a recurrent parent background. Previous functional analysis of BABY BOOM (BBM) genes from other species suggests that BBM plays a role in embryo development. To investigate the function of BABY BOOM-like genes identified from the ASGR compared with related genes not associated with the ASGR, ASGR-BBM-like and non-ASGR-BBM-like (N-ASGR-BBM-like) genes isolated from Cenchrus ciliaris were subjected to sequence analysis and expression characterization. Semi-quantitative RT-PCR with aposporous ovaries from different stages indicated that ASGR-BBM-like genes started transcription before pollination and the transcription was up-regulated upon pollination. In contrast, there was no expression of N-ASGR-BBM-like genes until one day after pollination. Moreover, the transcription level of N-ASGR-BBM-like was much lower than that of ASGR-BBM-like at this time point. Temporal and quantitative differences in transcript level between the two suggest that ASGR-BBM-like plays a role in parthenogenesis in buffelgrass.

Subjects/Keywords: Pennisetum squamulatum; Cenchrus ciliaris; apospory initiation; 454 sequencing; parthenogenesis; semi-quantitative RT-PCR; BBM-like gene

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Zeng, Y. (2014). Identification and characterization of apospory candidate genes in Pennisetum and Cenchrus. (Thesis). University of Georgia. Retrieved from http://hdl.handle.net/10724/26204

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Zeng, Yajuan. “Identification and characterization of apospory candidate genes in Pennisetum and Cenchrus.” 2014. Thesis, University of Georgia. Accessed April 17, 2021. http://hdl.handle.net/10724/26204.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Zeng, Yajuan. “Identification and characterization of apospory candidate genes in Pennisetum and Cenchrus.” 2014. Web. 17 Apr 2021.

Vancouver:

Zeng Y. Identification and characterization of apospory candidate genes in Pennisetum and Cenchrus. [Internet] [Thesis]. University of Georgia; 2014. [cited 2021 Apr 17]. Available from: http://hdl.handle.net/10724/26204.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Zeng Y. Identification and characterization of apospory candidate genes in Pennisetum and Cenchrus. [Thesis]. University of Georgia; 2014. Available from: http://hdl.handle.net/10724/26204

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Aristotle University Of Thessaloniki (AUTH); Αριστοτέλειο Πανεπιστήμιο Θεσσαλονίκης (ΑΠΘ)

28. Maniatsi, Stefania. Η παρθενογένεση στην Artemia: μοριακή προσέγγιση.

Degree: 2011, Aristotle University Of Thessaloniki (AUTH); Αριστοτέλειο Πανεπιστήμιο Θεσσαλονίκης (ΑΠΘ)

URL: http://hdl.handle.net/10442/hedi/30182

► Ενώ η πλειονότητα των ευκαρυωτικών οργανισμών αναπαράγονται μεφυλετικό τρόπο, υπάρχει μια μικρή κατηγορία διαφορετικών και ενδιαφερόντωνοργανισμών που παρεκκλίνουν από το γενικό κανόνα, καθώς αναπαράγονταιχρησιμοποιώντας εναλλακτικούς… (more)

▼ Ενώ η πλειονότητα των ευκαρυωτικών οργανισμών αναπαράγονται μεφυλετικό τρόπο, υπάρχει μια μικρή κατηγορία διαφορετικών και ενδιαφερόντωνοργανισμών που παρεκκλίνουν από το γενικό κανόνα, καθώς αναπαράγονταιχρησιμοποιώντας εναλλακτικούς τρόπους. Εν συντομία, αυτές οιαναπαραγωγικές στρατηγικές εκτείνονται από την εναλλαγή του φυλετικού καιτου αφυλετικού τρόπου κατά τον κύκλο ζωής του οργανισμού έως την αυστηρήκλωνική αναπαραγωγή κατά την οποία δε συμβαίνει ανασυνδυασμός.Η αφυλετική αναπαραγωγή πιστεύεται ότι είναι σπάνια στο ζωικόβασίλειο. Γενικά, οι αφυλετικοί οργανισμοί θεωρούνται εξελικτικά αδιέξοδαγιατί έρχονται αντιμέτωποι με σημαντικά μειονεκτήματα, τόσο άμεσα όσο καιέμμεσα, που απειλούν την εξελικτική τους συνέχεια. Ωστόσο, διάφοροιγενετικοί παράγοντες που σχετίζονται με την αφυλετική αναπαραγωγή, όπως γιαπαράδειγμα η πολυπλοειδία, μπορούν πιθανόν να ανανεώσουν τη γονιδιακήδεξαμενή των κλωνικών στελεχών, συμβάλλοντας θετικά στην προσαρμογή τους.Στην παρούσα εργασία, η Artemia χρησιμοποιήθηκε ως οργανισμός-μοντέλο για τον έλεγχο των βαθμών πλοειδίας, τη σύσταση τωνπαρθενογενετικών πληθυσμών καθώς και τη προέλευση και το βαθμό τηςκλωνικής ποικιλότητας των παρθενογενετικών στελεχών. Επιπλέον, έγινεπροσδιορισμός του φαινομένου της παρθενογένεσης και της πολυπλοειδίαςμέσα στο γένος, λαμβάνοντας υπόψη τις φυλογενετικές σχέσεις και ταγεωγραφικά πρότυπα των παρθενογενετικών πληθυσμών και των συγγενικώντους αμφιγονικών ειδών.Το γένος Artemia περιλαμβάνει οργανισμούς που ανήκουν στακαρκινοειδή και ειδικότερα στην τάξη των ανοστράκων, οι οποίοι έχουνπαγκόσμια εξάπλωση (εκτός της Ανταρκτικής), και είναι ευρύτερα γνωστοί ως«γαρίδες της άλμης». Μέχρι τον προηγούμενο αιώνα, οι περισσότερες μελέτεςπου είχαν γίνει αφορούσαν τη βιολογία και την οικολογία της Artemia. Αν καιείναι περισσότερο γνωστή για τη χρήση της ως ζωντανή τροφή για θαλάσσιουςοργανισμούς και οργανισμούς του γλυκού νερού, από εξελικτικής άποψης,αυτό το είδος είναι εξίσου συναρπαστικό. Δύο διαφορετικοί τρόποιαναπαραγωγής μπορούν να ανιχνευτούν: η φυλετική αναπαραγωγή και ηπαρθενογένεση. Πιο αναλυτικά, το γένος Artemia περιλαμβάνει έξι καλάχαρακτηρισμένα αμφιγονικά είδη και έναν μεγάλο αριθμό παρθενογενετικών στελεχών, τα οποία εμφανίζουν διαφορετικούς βαθμούς πλοειδίας (2n – 5n).Όσον αφορά τα παρθενογενετικά στελέχη, τα πολυπλοειδή θεωρούνταιαπομικτικά σε αντίθεση με τα αυτομικτικά διπλοειδή στελέχη. Επίσης, όλα ταάτομα Artemia, ανεξάρτητα από τον τρόπο αναπαραγωγής τους, μπορούν ναπαράγουν ναυπλίους (ωοζωοτοκία) ή εγκυστομένα διαπαυσιακά έμβρυα, τιςκύστεις (ωοτοκία). Η απελευθέρωση των ναυπλίων επιτρέπει τη ταχεία ανάπτυξητου πληθυσμού όταν οι συνθήκες το επιτρέπουν, ενώ, όταν πάψουν να είναιευνοϊκές, η ωοτοκία εξασφαλίζει την επιβίωση του πληθυσμού. Επιπλέον, οικύστεις μπορούν να μεταφερθούν μέσω των ζωικών οργανισμών, του ανέμου ήτου ανθρώπου, επιτρέποντας την εξάπλωση του οργανισμού σε άλλαενδιαιτήματα (παθητική μεταφορά). Τέλος, η συσσώρευση των κύστεων στονπυθμένα των υδατοσυλλογών έχει ως αποτέλεσμα τη δημιουργία «τραπεζών» μεδιαπαυσιακές μορφές.…

Subjects/Keywords: Αφυλετικότητα; Γεωγραφική παρθενογένεση; Κυτταρομετρία ροής; Μικροδορυφορικοί δείκτες; Αρτέμια; Asexuality; Geographic parthenogenesis; Flow cytometry; Microsatellite markers; Artemia; Wolbachia

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Maniatsi, S. (2011). Η παρθενογένεση στην Artemia: μοριακή προσέγγιση. (Thesis). Aristotle University Of Thessaloniki (AUTH); Αριστοτέλειο Πανεπιστήμιο Θεσσαλονίκης (ΑΠΘ). Retrieved from http://hdl.handle.net/10442/hedi/30182

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Maniatsi, Stefania. “Η παρθενογένεση στην Artemia: μοριακή προσέγγιση.” 2011. Thesis, Aristotle University Of Thessaloniki (AUTH); Αριστοτέλειο Πανεπιστήμιο Θεσσαλονίκης (ΑΠΘ). Accessed April 17, 2021. http://hdl.handle.net/10442/hedi/30182.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Maniatsi, Stefania. “Η παρθενογένεση στην Artemia: μοριακή προσέγγιση.” 2011. Web. 17 Apr 2021.

Vancouver:

Maniatsi S. Η παρθενογένεση στην Artemia: μοριακή προσέγγιση. [Internet] [Thesis]. Aristotle University Of Thessaloniki (AUTH); Αριστοτέλειο Πανεπιστήμιο Θεσσαλονίκης (ΑΠΘ); 2011. [cited 2021 Apr 17]. Available from: http://hdl.handle.net/10442/hedi/30182.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Maniatsi S. Η παρθενογένεση στην Artemia: μοριακή προσέγγιση. [Thesis]. Aristotle University Of Thessaloniki (AUTH); Αριστοτέλειο Πανεπιστήμιο Θεσσαλονίκης (ΑΠΘ); 2011. Available from: http://hdl.handle.net/10442/hedi/30182

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Utah State University

29. Sherwood, David Alan. A Simple Metabolic Switch May Activate Apomixis in Arabidopsis thaliana.

Degree: PhD, Plants, Soils, and Climate, 2018, Utah State University

URL: https://digitalcommons.usu.edu/etd/7409

► Apomixis, asexual or clonal seed production in plants, can decrease the cost of producing hybrid seed and enable currently open pollinated crops to be… (more)

Subjects/Keywords: apomeiosis; brassinosteroid; expression profiling; parthenogenesis; TARGET OF RAPAMYCIN (TOR); Agronomy and Crop Sciences; Molecular Biology; Plant Sciences

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Sherwood, D. A. (2018). A Simple Metabolic Switch May Activate Apomixis in Arabidopsis thaliana. (Doctoral Dissertation). Utah State University. Retrieved from https://digitalcommons.usu.edu/etd/7409

Chicago Manual of Style (16^th Edition):

Sherwood, David Alan. “A Simple Metabolic Switch May Activate Apomixis in Arabidopsis thaliana.” 2018. Doctoral Dissertation, Utah State University. Accessed April 17, 2021. https://digitalcommons.usu.edu/etd/7409.

MLA Handbook (7^th Edition):

Sherwood, David Alan. “A Simple Metabolic Switch May Activate Apomixis in Arabidopsis thaliana.” 2018. Web. 17 Apr 2021.

Vancouver:

Sherwood DA. A Simple Metabolic Switch May Activate Apomixis in Arabidopsis thaliana. [Internet] [Doctoral dissertation]. Utah State University; 2018. [cited 2021 Apr 17]. Available from: https://digitalcommons.usu.edu/etd/7409.

Council of Science Editors:

Sherwood DA. A Simple Metabolic Switch May Activate Apomixis in Arabidopsis thaliana. [Doctoral Dissertation]. Utah State University; 2018. Available from: https://digitalcommons.usu.edu/etd/7409

University of Vienna

30. Cosendai, Anne-Caroline. Geographical parthenogenesis.

Degree: 2010, University of Vienna

URL: http://othes.univie.ac.at/11233/

► Diese Dissertation hat zum Ziel, das Phänomen der geographische Parthenogenese (GP) zu verstehen. GP bedeutet, dass sexuelle und asexuelle Populationen desselben Artkomplexes nicht die gleiche… (more)

▼ Diese Dissertation hat zum Ziel, das Phänomen der geographische Parthenogenese (GP) zu verstehen. GP bedeutet, dass sexuelle und asexuelle Populationen desselben Artkomplexes nicht die gleiche geographische Verbreitung aufweisen. Im allgemeinen besetzen asexuelle Populationen höhere Lagen und Breitengrade, und sind weiter verbreitet. Geographische Parthenogenese kombiniert verschiedene Aspekte, die für das Verständnis der Verbreiungsmuster betrachtet werden müssen: das Repoduktion-System, Polyploidisierung, genetische Diversität und die Fähigkeit zur Kolonisierung. Die alpine Art Ranunculus kuepferi ist als Modell zum Studium der geographischen Parthenogenese geeignet, da polyploide, asexuelle Populationen im gesamten Alpengebiet weit verbreitet sind, während die diploiden sexuellen Populationen auf glaziale Refugien beschränkt sind. Ich habe verschiedene Formen der Reproduktion und die Stabilität der Zytotypen mittels Flow Cytometry (FC) und Durchflusszytometrie an Samen (FCSS) untersucht. Die Herkunft der Polyploidisierung und die genetische Vielfalt der Populationen wurde mit molekular-biologischen Methoden, mit Amplified Fragment Length Polymorphism (AFLPs; dominante Marker) und Mikrosatelliten (SSR; kodominanten Marker) analysiert. Diese Studien wurden an 60 Populationen aus dem ganzen Verbreitungsgebiet dieser Art durchgeführt. All diese Marker haben dazu beigetragen, Aufschluss über die genetische Struktur und den Einfluss der Reproduktion auf die Entstehung von geographischer Parthenogenese zu erhalten. Die drei Kapitel der Arbeit behandeln die verschiedenen Aspekte wie folgt: Die Stabilität der Zytotypen wurde mittels FC, der Fortpflanzungsmodus mittels FCSS bestimmt. Diese Analyse ergab, dass die diploiden Populationen stabil und voll sexuell bleiben. Triploide individuen kommen in der Kontaktzone vor und sind ein Produkt von Rückkreuzungen zwischen diploiden und tetraploiden Populationen. Außerhalb der Kontaktzone weisen in tetraploiden Populationen c. 30% der Samen triploide Embryos auf, aber triploide erwachsene Pflanzen treten nur einmal im ganzen tetraploiden Verbreitungsgebiet auf. Die Mehrheit der Tetraploiden behält eine stabile Ploidiestufe mittels gametophytischer Apomixis, wobei die Bildung des Endosperms entweder pseudogam oder autonom erfolgt. Einige wenige Prozent der Samen der tetraploiden Pflanzen sind auf sexuellem Weg entstanden. Polyploidisierung kann eine Erklärung geben, warum Apomixis in der Art Ranunculus kuepferi entstanden ist. Die autopolyploide Herkunft der Tetraploiden wurde mittels Bayesian-Analyse der Populations-Struktur (BAPS) Analyse der SSRs nachgewiesen. Damit konnte gezeigt werden, dass die tetraploiden aus diploiden Populationen ohne den Beitrag eines anderen Genoms entstanden sind. Die tetraploiden entstanden mehrfach von diploiden Populationen, da sie fast keine genetischen Unterschiede und nur wenige neue SSRs-Allele zeigen. AFLPs bestätigen in einer Neighbor Joining Analyse, dass diploide und tetraploide Populationen den gleichen Gen-Pool aufweisen. Daher ist der…

Subjects/Keywords: 30.00 Naturwissenschaften allgemein: Allgemeines; 30.99 Naturwissenschaften allgemein: Sonstiges; Geographical parthenogenesis / Evolution / Reproduction system / Apomixis / Autopolyploidy / Genetic diversity and Ranunculus kuepferi; Geographical parthenogenesis / Evolution / Reproduction system / Apomixis / Autopolyploidy / Genetic diversity and Ranunculus kuepferi

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Cosendai, A. (2010). Geographical parthenogenesis. (Thesis). University of Vienna. Retrieved from http://othes.univie.ac.at/11233/

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Cosendai, Anne-Caroline. “Geographical parthenogenesis.” 2010. Thesis, University of Vienna. Accessed April 17, 2021. http://othes.univie.ac.at/11233/.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Cosendai, Anne-Caroline. “Geographical parthenogenesis.” 2010. Web. 17 Apr 2021.

Vancouver:

Cosendai A. Geographical parthenogenesis. [Internet] [Thesis]. University of Vienna; 2010. [cited 2021 Apr 17]. Available from: http://othes.univie.ac.at/11233/.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Cosendai A. Geographical parthenogenesis. [Thesis]. University of Vienna; 2010. Available from: http://othes.univie.ac.at/11233/

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

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