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You searched for subject:(PROTEIN ISOFORMS analysis). Showing records 1 – 3 of 3 total matches.

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Université Paris-Sud – Paris XI

1. Sagne, Charlotte. Polymorphisms in G-quadruplex regions of the TP53 tumour suppressor gene : Impact on cancer susceptibility and expression of p53 N-terminal isoforms : Polymorphismes situés dans les régions de type G-quadruplexe du gène suppresseur de tumeur TP53 : Impact sur la susceptibilité au cancer et l’expression des isoformes en N-terminal de p53.

Degree: Docteur es, Biologie, 2013, Université Paris-Sud – Paris XI

Le gène TP53 est extrêmement polymorphique avec 85 polymorphismes décrits. Certains de ces polymorphismes sont associés à une augmentation du risque de cancer, par exemple rs10425222 peut moduler les fonctions de p53. Cependant, pour d’autres, comme le rs17878362 qui est le polymorphisme intronique le plus étudié, leur association avec une augmentation du riques au cancer est controversée.Pour analyser l’association entre le polymorphisme rs17878362 et la susceptibilité au cancer, nous avons analysé son rôle dans des contextes de cancers sporadiques et familiaux. Les résultats obtenus pour le polymorphisme rs17878362 sont paradoxaux avec une augmentation des cancers sporadiques associée avec le génotype A2A2 alors que l’allèle A2 est associé avec un effet « protectif » chez les patients atteints du syndrome de Li-Fraumeni porteurs d’une mutation germinale de TP53 situé sur l’haplotype A1. Ces observations suggèrent que des haplotypes spécifiques de TP53 pourraient moduler les capacités suppressives de p53. Une hypothèse possible est que les différents haplotypes de TP53 présenteraienrt des mutations somatiques à des fréquences différentes dans la population.De plus, le gène TP53 exprime différentes isoformes, comme le D40p53, inhibant l’activité suppressive de p53. Le D40p53 peut être produite par le maintien de l’intron 2 par épissage alternatif. Nous avons montré que les G-quadruplexes, des structures tridimensionnelles formées dans des régions riches en G, sont formés dans l’intron 3 et régulent la rétention de l’intron 2 et la formation du transcrit p53I2. Nous avons aussi observé que le polymorphisme rs1652785 (localisé dans l’intron 2) semble réguler la stabilité du p53I2. Ces résultats suggèrent que les polymorphismes de TP53 localisés dans une région de 412 pb située entre l’exon 2 et l’exon 4 régulent l’expression des isoformes de p53 dans une séquence temporelle d’évènements en modulant la formation des pré-ARNm (rs17878362), la stabilité des ARNm (rs1642785) et les fonctions protéiques (rs10425222).L’expression des isoformes de p53 est donc finement régulée par des mécanismes impliquant les polymorphismes de TP53 qui sont aussi associés avec une altération dans la susceptibilité au cancer.

The TP53 gene is a highly polymorphic gene with 85 polymorphisms described. Some of these have been associated with an increase of cancer susceptibility, for example rs10425222 that can modulate certain p53 activities. However for others such as rs17878362, the most studied intronic polymorphism, the association with cancer risk is more controversial. To investigate the influence of rs17878362 on cancer susceptibility, we analysed its role in sporadic and familial contexts. The results are paradoxical with an increase of sporadic cancer associated with the rs17878362 A2A2 genotype whereas the rs17878362 A2 allele is associated with a “protective” effect in the context of Li-Fraumeni patients carrying a TP53 germline mutation on an A1 haplotype. These observations suggest that specific TP53 haplotypes could modulate…

Advisors/Committee Members: Hall, Janet (thesis director).

Subjects/Keywords: Protéine suppresseur de tumeur; P53; Isoformes; Polymorphismes; Rs17878362; Épissage alternatif; Structure de type G-quadruplexe; Syndrome de Li-Fraumeni; Méta-analyse; Tumour suppressor protein; P53; Isoforms; Polymorphisms; Rs17878362; Alternative splicing; G-quadruplex structure; Li-Fraumeni syndrome; Meta-analysis

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APA (6th Edition):

Sagne, C. (2013). Polymorphisms in G-quadruplex regions of the TP53 tumour suppressor gene : Impact on cancer susceptibility and expression of p53 N-terminal isoforms : Polymorphismes situés dans les régions de type G-quadruplexe du gène suppresseur de tumeur TP53 : Impact sur la susceptibilité au cancer et l’expression des isoformes en N-terminal de p53. (Doctoral Dissertation). Université Paris-Sud – Paris XI. Retrieved from http://www.theses.fr/2013PA11T072

Chicago Manual of Style (16th Edition):

Sagne, Charlotte. “Polymorphisms in G-quadruplex regions of the TP53 tumour suppressor gene : Impact on cancer susceptibility and expression of p53 N-terminal isoforms : Polymorphismes situés dans les régions de type G-quadruplexe du gène suppresseur de tumeur TP53 : Impact sur la susceptibilité au cancer et l’expression des isoformes en N-terminal de p53.” 2013. Doctoral Dissertation, Université Paris-Sud – Paris XI. Accessed November 29, 2020. http://www.theses.fr/2013PA11T072.

MLA Handbook (7th Edition):

Sagne, Charlotte. “Polymorphisms in G-quadruplex regions of the TP53 tumour suppressor gene : Impact on cancer susceptibility and expression of p53 N-terminal isoforms : Polymorphismes situés dans les régions de type G-quadruplexe du gène suppresseur de tumeur TP53 : Impact sur la susceptibilité au cancer et l’expression des isoformes en N-terminal de p53.” 2013. Web. 29 Nov 2020.

Vancouver:

Sagne C. Polymorphisms in G-quadruplex regions of the TP53 tumour suppressor gene : Impact on cancer susceptibility and expression of p53 N-terminal isoforms : Polymorphismes situés dans les régions de type G-quadruplexe du gène suppresseur de tumeur TP53 : Impact sur la susceptibilité au cancer et l’expression des isoformes en N-terminal de p53. [Internet] [Doctoral dissertation]. Université Paris-Sud – Paris XI; 2013. [cited 2020 Nov 29]. Available from: http://www.theses.fr/2013PA11T072.

Council of Science Editors:

Sagne C. Polymorphisms in G-quadruplex regions of the TP53 tumour suppressor gene : Impact on cancer susceptibility and expression of p53 N-terminal isoforms : Polymorphismes situés dans les régions de type G-quadruplexe du gène suppresseur de tumeur TP53 : Impact sur la susceptibilité au cancer et l’expression des isoformes en N-terminal de p53. [Doctoral Dissertation]. Université Paris-Sud – Paris XI; 2013. Available from: http://www.theses.fr/2013PA11T072


Indian Institute of Science

2. Grover, Richa. Translational Control Of p53 And Its Isoform By Internal Initiation.

Degree: PhD, Faculty of Science, 2010, Indian Institute of Science

Tumor suppressor p53, the guardian of the genome, has been intensely studied molecule owing to its central role in maintaining cellular integrity. While the level of p53 protein is maintained low in unstressed conditions, there is a rapid increase in the functional p53 protein levels during stress conditions. It is now well documented in literature that p53 protein accumulates in the cells following DNA damage by posttranslational modifications leading to increased stability and half life of protein. Additionally, recent studies have also highlighted the significance of increased p53 translation during stress conditions. Interestingly, an alternative initiation codon has been shown to be present within the coding region of p53 mRNA. Translation initiation from this internal AUG results in an N-terminally truncated p53 isoform, described as ΔN-p53. However, the mechanisms underlying co-translational regulation of p53 and ΔN-p53 are still poorly understood. Studies have suggested that synthesis of both p53 and its ΔN-p53 isoform is regulated during cell cycle and also stress and cell-type specific manner. Interestingly, reports also demonstrate continued synthesis of both p53 isoforms during stress conditions. In contrast, global rates of cap-dependent translation initiation are shown to be reduced during stress conditions. This translation attenuation is observed mainly due to restricted availability of critical initiation factors. Interestingly, preferential synthesis of a vital pool of survival factors persists even during these circumstances. Studies have suggested that this selective translation is mediated via alternative mechanisms of translation initiation. One of the important mechanisms used for protein synthesis during these conditions is internal initiation. In this mechanism, the ribosomes are recruited to a complex RNA structural element known as ‘Internal Ribosome Entry Site (IRES)’, generally present in the 5’ untranslated region (UTR) of mRNA. Therefore, it is possible that the translation of p53 and ΔN-p53 could also be regulated by IRES mediated translation, especially during stress conditions. In this thesis the role of internal initiation in translational control of p53 and ΔN-p53 has been investigated. Additionally, the putative secondary structure of p53 IRES RNA has been determined. Further, it has been shown that polypyrimidine tract binding (PTB) protein acts as an important regulator of p53 IRES activities. The probable mechanism of action of PTB protein has also been investigated. The results suggest that interaction with PTB alters the p53 IRES conformation which could facilitate translation initiation. Finally, the possible physiological significance of existence of p53 IRES elements has been addressed. In the first part of the thesis, the presence of internal ribosome entry site within p53 mRNA has been investigated. As a first step, the 5’UTRs mediating the translation of both p53 and ΔN-p53 were cloned in the intercistronic regions of bicistronic constructs. Results of in vivo… Advisors/Committee Members: Das, Saumitra (advisor).

Subjects/Keywords: Ribonucleic Acid (RNA); Proteins; RNA Viruses; p53 Protein; p53 Protein Isoforms; p53 RNA - Structural Analysis; Protein Translation; Polypyrimidine Tract Binding Protein; Protein Regulation; IRES RNA; PTB Binding; p53 mRNA; Biochemical Genetics

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6th Edition):

Grover, R. (2010). Translational Control Of p53 And Its Isoform By Internal Initiation. (Doctoral Dissertation). Indian Institute of Science. Retrieved from http://etd.iisc.ac.in/handle/2005/764

Chicago Manual of Style (16th Edition):

Grover, Richa. “Translational Control Of p53 And Its Isoform By Internal Initiation.” 2010. Doctoral Dissertation, Indian Institute of Science. Accessed November 29, 2020. http://etd.iisc.ac.in/handle/2005/764.

MLA Handbook (7th Edition):

Grover, Richa. “Translational Control Of p53 And Its Isoform By Internal Initiation.” 2010. Web. 29 Nov 2020.

Vancouver:

Grover R. Translational Control Of p53 And Its Isoform By Internal Initiation. [Internet] [Doctoral dissertation]. Indian Institute of Science; 2010. [cited 2020 Nov 29]. Available from: http://etd.iisc.ac.in/handle/2005/764.

Council of Science Editors:

Grover R. Translational Control Of p53 And Its Isoform By Internal Initiation. [Doctoral Dissertation]. Indian Institute of Science; 2010. Available from: http://etd.iisc.ac.in/handle/2005/764


University of Florida

3. Daniels, Kellye K., 1967-. Properties of high-affinity L-Glutamate transport in glial- and neuronal-enriched fractions from rat brain Kellye K. Daniels.

Degree: 1997, University of Florida

Subjects/Keywords: Brain; Enzymes; Neuroglia; Neurons; Phorbol esters; Phosphatases; Phosphorylation; Protein isoforms; Rats; Synaptosomes; Biological Transport  – physiology ( mesh ); Brain ( mesh ); Cell Aging ( mesh ); Department of Neuroscience thesis Ph.D ( mesh ); Glutamates  – metabolism ( mesh ); Glutamates  – physiology ( mesh ); Neuroglia ( mesh ); Neurons ( mesh ); Neurotransmitter Uptake Inhibitors  – pharmacokinetics ( mesh ); Phosphorylation ( mesh ); Protein Kinase C ( mesh ); Rats ( mesh ); Research ( mesh ); Subcellular Fractions  – analysis ( mesh ); Subcellular Fractions  – isolation &; purification ( mesh )

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6th Edition):

Daniels, Kellye K., 1. (1997). Properties of high-affinity L-Glutamate transport in glial- and neuronal-enriched fractions from rat brain Kellye K. Daniels. (Thesis). University of Florida. Retrieved from https://ufdc.ufl.edu/AA00031499

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16th Edition):

Daniels, Kellye K., 1967-. “Properties of high-affinity L-Glutamate transport in glial- and neuronal-enriched fractions from rat brain Kellye K. Daniels.” 1997. Thesis, University of Florida. Accessed November 29, 2020. https://ufdc.ufl.edu/AA00031499.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7th Edition):

Daniels, Kellye K., 1967-. “Properties of high-affinity L-Glutamate transport in glial- and neuronal-enriched fractions from rat brain Kellye K. Daniels.” 1997. Web. 29 Nov 2020.

Vancouver:

Daniels, Kellye K. 1. Properties of high-affinity L-Glutamate transport in glial- and neuronal-enriched fractions from rat brain Kellye K. Daniels. [Internet] [Thesis]. University of Florida; 1997. [cited 2020 Nov 29]. Available from: https://ufdc.ufl.edu/AA00031499.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Daniels, Kellye K. 1. Properties of high-affinity L-Glutamate transport in glial- and neuronal-enriched fractions from rat brain Kellye K. Daniels. [Thesis]. University of Florida; 1997. Available from: https://ufdc.ufl.edu/AA00031499

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

.