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NSYSU
1.
Chi, Yi-Chun.
A Comparison of PLGA and Titanium Plates When Used as a Fixation Material in Fractured Femurs of Diabetic Rats.
Degree: Master, Biological Sciences, 2010, NSYSU
URL: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0817110-020857 
► Titanium bone plates and screws are commonly used in oral and maxillofacial surgery for internal fixation. Although titanium plate is good for fixation of bone…
(more)
▼ Titanium bone plates and screws are commonly used in oral and maxillofacial surgery for internal fixation. Although titanium plate is good for fixation of bone fractures, there are still somemany disadvantages of the material. Biodegradable materials are considered to be good alternatives for treatment of the facial bone fractures.
Poor wound healing in diabetic animals and human was well known.
The aims of the studies were to compare the strength and tissue reaction of the poly lactic-co-glycolic acid ï¼PLGAï¼ with titanium plates used as fixation material in fractured-femora of diabetic and normal animals.
The results of the studies show that the wounds of the control group healed quite well and there was no discharge around the wound. However, bullas formation was found in all the diabetic wounds fixed with
PLGA. There was fistula with pus and gas discharge around the bullas. In the control group, the diameter of the
PLGA-fixed femur was larger than the titanium-fixed femura. There was a large amount of callus formation around the
PLGA-fixed fracture femur.
At the 20th day, the defects of both groups of the
PLGA treated were not healed. A large amount of new bone formation in normal titanium-treated rats was found, while in diabetic rats, mainly a fibrous tissue reaction.
At the 40th day, the diabetic
PLGA-fixed femora were poorly healed with a large amount of fibrous tissue. Similarly, after 40 days in the titanium-fixed femora of diabetic rats, extensive fibrosis of the defect was also present. In normal
PLGA-fixed fractured femora, there was a large amount of cartilage present around the defect. Most of the normal
PLGA-fixed fractured femora were distorted in shape.
At the 40th day, lamellar bone formation was found around the screw in normal titanium-fixed femora and the defect was healed.
In conclusion, the
PLGA plate is not suitable for fixation in the fractured-femora of the rats in either normal or diabetic groups when compared with those from titanium plate treat groups. It is because of less strength of the
PLGA plate and the material easily cause tissue reaction in the rat which result in the formation of large amount of fibrous tissue around the
PLGA screw instead of bone.
Advisors/Committee Members: Keng-Lian OU (chair), Yih-Chuen Shyng (committee member), Chau-Hsiang Wang (chair), David Chao (committee member).
Subjects/Keywords: Diabetic; PLGA
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APA (6th Edition):
Chi, Y. (2010). A Comparison of PLGA and Titanium Plates When Used as a Fixation Material in Fractured Femurs of Diabetic Rats. (Thesis). NSYSU. Retrieved from http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0817110-020857
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Chi, Yi-Chun. “A Comparison of PLGA and Titanium Plates When Used as a Fixation Material in Fractured Femurs of Diabetic Rats.” 2010. Thesis, NSYSU. Accessed March 04, 2021.
http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0817110-020857.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Chi, Yi-Chun. “A Comparison of PLGA and Titanium Plates When Used as a Fixation Material in Fractured Femurs of Diabetic Rats.” 2010. Web. 04 Mar 2021.
Vancouver:
Chi Y. A Comparison of PLGA and Titanium Plates When Used as a Fixation Material in Fractured Femurs of Diabetic Rats. [Internet] [Thesis]. NSYSU; 2010. [cited 2021 Mar 04].
Available from: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0817110-020857.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Chi Y. A Comparison of PLGA and Titanium Plates When Used as a Fixation Material in Fractured Femurs of Diabetic Rats. [Thesis]. NSYSU; 2010. Available from: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0817110-020857
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
2.
Thuane Castro Frabel do Nascimento.
DESENVOLVIMENTO E AVALIAÇÃO FARMACOCINÉTICA DE NANOPARTÍCULAS DE PLGA E PLGA-PEG CONTENDO CURCUMINA.
Degree: 2011, UNICENTRO – Universidade Estadual do Centro Oeste
URL: http://tede.unicentro.br/tde_busca/arquivo.php?codArquivo=120
► As nanopartículas poliméricas apresentam grande importância na área farmacêutica em virtude de serem sistemas coloidais que possuem interessantes propriedades físicoquímicas, tais como o tamanho reduzido,…
(more)
▼ As nanopartículas poliméricas apresentam grande importância na área farmacêutica em virtude de serem sistemas coloidais que possuem interessantes propriedades físicoquímicas, tais como o tamanho reduzido, a ampla área superficial, carga superficial, que as tornam eficientes sistemas para aplicação na liberação controlada de fármacos. A curcumina é um pigmento amarelo presente na Curcuma longa que possui baixa toxicidade e uma ampla faixa de atividades farmacológicas, estando entre os mais promissores e eficazes agentes quimiopreventivos e/ou antitumorais. Porém o seu uso terapêutico tem sido limitado devido a sua baixa solubilidade aquosa, sua alta velocidade de decomposição em pH neutro ou básico, além do rápido metabolismo e eliminação sistêmica, resultando em baixa biodisponibilidade. Neste trabalho obteve-se nanopartículas de ácido poli-(láctico-co-glicólico) (PLGA) e de blendas de PLGA com polietilenoglicol (PEG) contendo curcumina através da técnica de emulsificaçãoevaporação do solvente, com o objetivo de melhorar suas propriedades farmacocinéticas. Após a validação de um método por cromatografia líquida de alta eficiência (CLAE) para a quantificação da curcumina, as nanopartículas foram avaliadas quanto ao diâmetro médio e eficiência de encapsulação. Ambas as formulações obtiveram eficiência de encapsulação superior a 75% e o diâmetro médio não foi superior a 200 nm. O estudo de liberação in vitro mostrou que as nanopartículas sustentam a liberação da curcumina, e que a presença do PEG na formulação contribui para o aumento na velocidade de liberação da curcumina. Um método por cromatografia líquida acoplada a espectrometria de massas foi desenvolvido e validado e se mostrou altamente sensível, reprodutível e específico para análise de curcumina em plasma de rato. Após administração oral em ratos, as formulações de nanopartículas de PLGA e ii blendas de PLGA-PEG foram capazes de manter uma liberação sustentada da curcumina, com resultados significativamente diferentes entre as formulações. As nanopartículas de PLGA e PLGA-PEG aumentaram o tempo de meia vida da curcumina em aproximadamente 4 e 6 h, respectivamente. Comparando-se com a suspensão aquosa de curcumina, o pico máximo de concentração plasmática da curcumina a partir das nanopartículas de PLGA e PLGA-PEG foi 2,9 e 7,4 vezes superior, respectivamente. A distribuição e o metabolismo da curcumina foi reduzido quando carreada pelas nanopartículas, principalmente pelas nanopartículas de PLGA-PEG. A biodisponibilidade da curcumina encapsulada em nanopartículas de PLGA-PEG foi 3,5 vezes superior em relação a encapsulada em nanopartículas de PLGA. Comparado com a suspensão aquosa de curcumina, as nanopartículas de PLGA e PLGA-PEG aumentaram a biodisponibilidade em 15,6 e 55,4 vezes, respectivamente. Estes resultados sugerem que nanopartículas de PLGA e principalmente de PLGA-PEG são promissores carreadores de curcumina para administração oral.
The polymeric nanoparticles present great importance in the pharmaceutical field due to be colloidal systems,…
Advisors/Committee Members: Rubiana Mara Mainardes.
Subjects/Keywords: Curcumina; Biodisponibilidade; LC-MS/MS; Nanopartículas, PLGA; PLGA-PEG; Curcumin; Bioavailability; LC-MS/MS; Nanoparticles; PLGA; PLGA-PEG; FARMACIA
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APA (6th Edition):
Nascimento, T. C. F. d. (2011). DESENVOLVIMENTO E AVALIAÇÃO FARMACOCINÉTICA DE NANOPARTÍCULAS DE PLGA E PLGA-PEG CONTENDO CURCUMINA. (Thesis). UNICENTRO – Universidade Estadual do Centro Oeste. Retrieved from http://tede.unicentro.br/tde_busca/arquivo.php?codArquivo=120
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Nascimento, Thuane Castro Frabel do. “DESENVOLVIMENTO E AVALIAÇÃO FARMACOCINÉTICA DE NANOPARTÍCULAS DE PLGA E PLGA-PEG CONTENDO CURCUMINA.” 2011. Thesis, UNICENTRO – Universidade Estadual do Centro Oeste. Accessed March 04, 2021.
http://tede.unicentro.br/tde_busca/arquivo.php?codArquivo=120.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Nascimento, Thuane Castro Frabel do. “DESENVOLVIMENTO E AVALIAÇÃO FARMACOCINÉTICA DE NANOPARTÍCULAS DE PLGA E PLGA-PEG CONTENDO CURCUMINA.” 2011. Web. 04 Mar 2021.
Vancouver:
Nascimento TCFd. DESENVOLVIMENTO E AVALIAÇÃO FARMACOCINÉTICA DE NANOPARTÍCULAS DE PLGA E PLGA-PEG CONTENDO CURCUMINA. [Internet] [Thesis]. UNICENTRO – Universidade Estadual do Centro Oeste; 2011. [cited 2021 Mar 04].
Available from: http://tede.unicentro.br/tde_busca/arquivo.php?codArquivo=120.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Nascimento TCFd. DESENVOLVIMENTO E AVALIAÇÃO FARMACOCINÉTICA DE NANOPARTÍCULAS DE PLGA E PLGA-PEG CONTENDO CURCUMINA. [Thesis]. UNICENTRO – Universidade Estadual do Centro Oeste; 2011. Available from: http://tede.unicentro.br/tde_busca/arquivo.php?codArquivo=120
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
University of Saskatchewan
3.
Rafiei, Pedram 1982-.
PHARMACOKINETIC CHARACTERIZATION AND OPTIMIZATION OF POLY (LACTIDE-CO-GLYCOLIDE) NANOPARTICLES IN VIVO.
Degree: 2017, University of Saskatchewan
URL: http://hdl.handle.net/10388/7940
► To date, nanotechnology is used to modify drug delivery and confer desirable pharmacokinetics to drugs and improve pharmacodynamics. Polymeric nanoparticles of Poly (lactide-co-glycolide) (PLGA) are…
(more)
▼ To date, nanotechnology is used to modify drug delivery and confer desirable pharmacokinetics to drugs and improve pharmacodynamics. Polymeric nanoparticles of Poly (lactide-co-glycolide) (
PLGA) are proposed as suitable vehicles that desirably modify pharmacokinetics. The variability in
PLGA and nanoparticle fabrication techniques results in nanoparticles with variable characteristics. It is important to identify factors that significantly influence particle characteristics during nanoparticle preparation to fabricate nanoparticles with the desired properties. Factors like size, zeta potential, and drug loading ability influence fate of nanoparticle and loaded drug in the body.
An experimental factorial design based on the Taguchi robust model was used to evaluate the influence of preparation variables on nanoparticle characteristics. Docetaxel, an anticancer agent, was used in the design. Factors affecting nanoparticle properties with statistical significance were identified and models were built to predict particle characteristics. An optimized fabrication method was identified and used to prepare docetaxel-loaded
PLGA nanoparticles and docetaxel-loaded PEGylated
PLGA nanoparticles. Surface-modification with Poly (ethylene glycol) (PEG) conferred long-circulating properties to
PLGA nanoparticles. A mass spectrometric analysis method was developed and partially validated for detection and quantification of docetaxel in biologic/non-biologic samples.
Size, zeta potential, Poly-dispersity index, drug release, and cytotoxicity of un-modified and surface-modified nanoparticles were determined. Pharmacokinetics and bio-distribution of docetaxel loaded in nanoparticles and in free solution were evaluated in mice and compared.
PLGA and
PLGA-PEG nanoparticles had average diameters of around 120 and 180 nm, respectively, with negative zeta potential. They demonstrated a biphasic release profile and were cytotoxic to Hela cells. Nanoparticles modified docetaxel’s bio-distribution by increasing docetaxel’s area under the curve, half-life, and mean residence time in blood while decreasing systemic clearance and apparent volume of distribution. Particle size and surface characteristics likely caused the modifications in docetaxel’s pharmacokinetics.
In summary, nanoparticles modified docetaxel’s pharmacokinetics and bio-distribution. The relationship between nanoparticle properties and pharmacokinetic modifications can be established and used to design nanoparticles with intended characteristics that could intentionally change docetaxel’s pharmacokinetics. Models built from the Taguchi design offer suitable means to prepare nanoparticles with predicted characteristics.
Advisors/Committee Members: Haddadi, Azita, Blackburn, David, Alcorn, Jane, El-Anees, Anas, Badea, Ildiko, Harkness, Troy.
Subjects/Keywords: PLGA Nanoparticles; Pharmacokinetics; Docetaxel
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APA (6th Edition):
Rafiei, P. 1. (2017). PHARMACOKINETIC CHARACTERIZATION AND OPTIMIZATION OF POLY (LACTIDE-CO-GLYCOLIDE) NANOPARTICLES IN VIVO. (Thesis). University of Saskatchewan. Retrieved from http://hdl.handle.net/10388/7940
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Rafiei, Pedram 1982-. “PHARMACOKINETIC CHARACTERIZATION AND OPTIMIZATION OF POLY (LACTIDE-CO-GLYCOLIDE) NANOPARTICLES IN VIVO.” 2017. Thesis, University of Saskatchewan. Accessed March 04, 2021.
http://hdl.handle.net/10388/7940.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Rafiei, Pedram 1982-. “PHARMACOKINETIC CHARACTERIZATION AND OPTIMIZATION OF POLY (LACTIDE-CO-GLYCOLIDE) NANOPARTICLES IN VIVO.” 2017. Web. 04 Mar 2021.
Vancouver:
Rafiei P1. PHARMACOKINETIC CHARACTERIZATION AND OPTIMIZATION OF POLY (LACTIDE-CO-GLYCOLIDE) NANOPARTICLES IN VIVO. [Internet] [Thesis]. University of Saskatchewan; 2017. [cited 2021 Mar 04].
Available from: http://hdl.handle.net/10388/7940.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Rafiei P1. PHARMACOKINETIC CHARACTERIZATION AND OPTIMIZATION OF POLY (LACTIDE-CO-GLYCOLIDE) NANOPARTICLES IN VIVO. [Thesis]. University of Saskatchewan; 2017. Available from: http://hdl.handle.net/10388/7940
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Penn State University
4.
Kuo, Chung-fan.
Development Of A New Nano-scale Vehicle For Delivery Of Curcumin In Biological Systems.
Degree: 2014, Penn State University
URL: https://submit-etda.libraries.psu.edu/catalog/22459
► Curcumin, a natural compound extracted from turmeric, has anti-inflammatory, anti-oxidant and neuroprotective properties that make it an attractive therapeutic agent for treatment of disesaes such…
(more)
▼ Curcumin, a natural compound extracted from turmeric, has anti-inflammatory, anti-oxidant and neuroprotective properties that make it an attractive therapeutic agent for treatment of disesaes such as cancer and Alzheimer’s disease (AD). Its low water solubility, however, lowers its bioavailability and effectiveness in physiological environment. The goal of this study is to apply nanotechnology to carry curcumin by developing lipid/polymer hybrid nanoparticles which can encapsulate curcumin and improve its bioavailability. To this end, we fabricated and characterized lipid-enveloped poly (D, L-lactide-co-glycolic acid) nanoparticles (
PLGA NPs) as a delivery vehicle for curcumin. A modified nanoprecipitation technique was used to fabricate polymeric particles by addressing the injection rate of the polymer solution as one of parameters. Two different approaches were then applied to create lipid monolayer or bilayer coatings on the nanoparticles. We evaluated the effect of non-solvent systems, polymer concentration, injection rate, and polymer/drug ratio on the particle size. After parameter optimization, both lipid bilayer coating and lipid monolayer coating were applied to the curcumin-loaded
PLGA nanoparticles. Further examinations including size change, surface charge, colloidal stability, lipid shell stability, encapsulation efficiency, and release profile were conducted to evaluate the physicochemical properties of the lipid-coated curcumin-loaded
PLGA nanoparticles.
Advisors/Committee Members: Sheereen Majdzarringhalamaraghy, Thesis Advisor/Co-Advisor, Peter J Butler, Thesis Advisor/Co-Advisor, Mohammad Reza Abidian, Thesis Advisor/Co-Advisor.
Subjects/Keywords: Lipid-coated PLGA nanoparticles; Curcumin
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APA (6th Edition):
Kuo, C. (2014). Development Of A New Nano-scale Vehicle For Delivery Of Curcumin In Biological Systems. (Thesis). Penn State University. Retrieved from https://submit-etda.libraries.psu.edu/catalog/22459
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Kuo, Chung-fan. “Development Of A New Nano-scale Vehicle For Delivery Of Curcumin In Biological Systems.” 2014. Thesis, Penn State University. Accessed March 04, 2021.
https://submit-etda.libraries.psu.edu/catalog/22459.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Kuo, Chung-fan. “Development Of A New Nano-scale Vehicle For Delivery Of Curcumin In Biological Systems.” 2014. Web. 04 Mar 2021.
Vancouver:
Kuo C. Development Of A New Nano-scale Vehicle For Delivery Of Curcumin In Biological Systems. [Internet] [Thesis]. Penn State University; 2014. [cited 2021 Mar 04].
Available from: https://submit-etda.libraries.psu.edu/catalog/22459.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Kuo C. Development Of A New Nano-scale Vehicle For Delivery Of Curcumin In Biological Systems. [Thesis]. Penn State University; 2014. Available from: https://submit-etda.libraries.psu.edu/catalog/22459
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Louisiana State University
5.
Simon, Lacey Caroline.
Bioavailability of Alpha-Tocopherol Entrapped in Poly (lactide-co-glycolide) (PLGA) and PLGA-Chitosan Nanoparticles.
Degree: MSBAE, Engineering, 2014, Louisiana State University
URL: etd-04112014-185904
;
https://digitalcommons.lsu.edu/gradschool_theses/2290
► Alpha-tocopherol (áT) is a hydrophobic, antioxidant molecule shown to prevent or retard the effects of free radicals associated with diseases. It is hypothesized that the…
(more)
▼ Alpha-tocopherol (áT) is a hydrophobic, antioxidant molecule shown to prevent or retard the effects of free radicals associated with diseases. It is hypothesized that the bioavailability of áT can be improved when entrapped in PLGA (Poly lactide-co-glycolide) and PLGA-Chi (PLGA-Chitosan) nanoparticles and that the mucoadhesive properties of chitosan may enhance absorption more than PLGA alone. PLGA and PLGA-Chi NPs synthesized with PVA as surfactant were characterized by measuring entrapment efficiency, size, PDI, and zeta potential when suspended in DI water. Nanoparticle physical stability was evaluated via NP exposure to environmental pH ranging from 2.5-9. Chemical stability of entrapped áT was also investigated by exposing particles to simulated GI (gastro-intestinal) environments for 48 hours, and release kinetics were measured in these conditions for 72 hours. Pharmacokinetic studies were conducted by administering PLGA (áT) NPs, PLGA-Chi (áT) NPs, and free áT in a flour, water, and corn oil slurry via oral gavage in rats. Blood samples were collected over 72 hours. Entrapment efficiency was 95.4 ± 9.85%, for PLGA NPs and 77.95 ± 1.51% for PLGA-Chi NPs. The size and zeta potential of the two particle systems were 97.87 ± 2.63 nm and -36.2 ± 1.31 mV for PLGA(áT) NPs, and 134 ± 2.05 nm and 38.0 ± 2.90 mV for PLGA-Chi (áT) nanoparticles in DI water. PLGA(áT) NPs showed a change in size of only 4 nm in the pH range of 2.5-9, while PLGA-Chi(áT) nanoparticles revealed a 46 nm change in size in pH 2.5-9. Entrapped áT showed no degradation in GI conditions over 48 hours. Release kinetics also showed no release of the áT from either NP system over 72 hours. Bioavailability of nanodelivered áT was improved as indicated compared to the free áT up to 170% and 121% for PLGA and PLGA-Chi NPs, respectively. These findings revealed that both delivery systems increased bioavailability of áT, but chitosan did not improve uptake of áT, as hypothesized.
Subjects/Keywords: Alpha-tocopherol; Bioavailability; Nanoparticles; PLGA
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APA ·
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APA (6th Edition):
Simon, L. C. (2014). Bioavailability of Alpha-Tocopherol Entrapped in Poly (lactide-co-glycolide) (PLGA) and PLGA-Chitosan Nanoparticles. (Masters Thesis). Louisiana State University. Retrieved from etd-04112014-185904 ; https://digitalcommons.lsu.edu/gradschool_theses/2290
Chicago Manual of Style (16th Edition):
Simon, Lacey Caroline. “Bioavailability of Alpha-Tocopherol Entrapped in Poly (lactide-co-glycolide) (PLGA) and PLGA-Chitosan Nanoparticles.” 2014. Masters Thesis, Louisiana State University. Accessed March 04, 2021.
etd-04112014-185904 ; https://digitalcommons.lsu.edu/gradschool_theses/2290.
MLA Handbook (7th Edition):
Simon, Lacey Caroline. “Bioavailability of Alpha-Tocopherol Entrapped in Poly (lactide-co-glycolide) (PLGA) and PLGA-Chitosan Nanoparticles.” 2014. Web. 04 Mar 2021.
Vancouver:
Simon LC. Bioavailability of Alpha-Tocopherol Entrapped in Poly (lactide-co-glycolide) (PLGA) and PLGA-Chitosan Nanoparticles. [Internet] [Masters thesis]. Louisiana State University; 2014. [cited 2021 Mar 04].
Available from: etd-04112014-185904 ; https://digitalcommons.lsu.edu/gradschool_theses/2290.
Council of Science Editors:
Simon LC. Bioavailability of Alpha-Tocopherol Entrapped in Poly (lactide-co-glycolide) (PLGA) and PLGA-Chitosan Nanoparticles. [Masters Thesis]. Louisiana State University; 2014. Available from: etd-04112014-185904 ; https://digitalcommons.lsu.edu/gradschool_theses/2290
Louisiana State University
6.
Whaley, Meocha.
Physical Stability of Poly (lactic-co-glycolic acid) and PLGA/Chitosan Nanoparticles and Chemical Stability of Entrapped Alpha-Tocopherol and Lutein.
Degree: MSBAE, Engineering, 2014, Louisiana State University
URL: etd-07132014-234534
;
https://digitalcommons.lsu.edu/gradschool_theses/544
► The published benefits of polymeric nanoparticles as a system for antioxidant delivery have encompassed topics of improved oral delivery, bioavailability, and modified release to…
(more)
▼ The published benefits of polymeric nanoparticles as a system for antioxidant delivery have encompassed topics of improved oral delivery, bioavailability, and modified release to name a few. Generally accepted as safe polymers Poly(lactic-coglycolic acid) and the mucoadhesive chitosan were selected to entrap alpha-tocopherol and lutein. This study proposed to assess the physical stability of PLGA and Chi/PLGA nanoparticles with entrapped bioactives in varying biological suspension medias ( water, pbs, hbss and cell media). Also, to assess the chemical stability of the entrapped antioxidants lutein and alpha-tocopherol within the same media as deemed relevant for in-vitro and and in-vivo studies. Of the tested media, physical stability was not media dependent for the PLGA(AO) or PLGA/Chi (AO) particles. Neither the size nor the PDI appeared significantly affected by the media it was re-suspended. Entrapped alphatocopherol remained chemically stable throughout the time suspended in the various biological mediums. While the lutein emulsion stability decreased over time, the NP(L)demonstrated improved stability. Also, the entrapped lutein stability was not dependent on the selected medium. Identifying any potential effects of suspension media on polymeric nanoparticles and the entrapped bioactive affords the opportunity to improve analysis of more robust or complex nanoparticle studies. Also, presented are considerations that should be taken prior to design of an experiment.
Subjects/Keywords: nanoparticles; PLGA; chitosan; stability
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APA ·
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Manager
APA (6th Edition):
Whaley, M. (2014). Physical Stability of Poly (lactic-co-glycolic acid) and PLGA/Chitosan Nanoparticles and Chemical Stability of Entrapped Alpha-Tocopherol and Lutein. (Masters Thesis). Louisiana State University. Retrieved from etd-07132014-234534 ; https://digitalcommons.lsu.edu/gradschool_theses/544
Chicago Manual of Style (16th Edition):
Whaley, Meocha. “Physical Stability of Poly (lactic-co-glycolic acid) and PLGA/Chitosan Nanoparticles and Chemical Stability of Entrapped Alpha-Tocopherol and Lutein.” 2014. Masters Thesis, Louisiana State University. Accessed March 04, 2021.
etd-07132014-234534 ; https://digitalcommons.lsu.edu/gradschool_theses/544.
MLA Handbook (7th Edition):
Whaley, Meocha. “Physical Stability of Poly (lactic-co-glycolic acid) and PLGA/Chitosan Nanoparticles and Chemical Stability of Entrapped Alpha-Tocopherol and Lutein.” 2014. Web. 04 Mar 2021.
Vancouver:
Whaley M. Physical Stability of Poly (lactic-co-glycolic acid) and PLGA/Chitosan Nanoparticles and Chemical Stability of Entrapped Alpha-Tocopherol and Lutein. [Internet] [Masters thesis]. Louisiana State University; 2014. [cited 2021 Mar 04].
Available from: etd-07132014-234534 ; https://digitalcommons.lsu.edu/gradschool_theses/544.
Council of Science Editors:
Whaley M. Physical Stability of Poly (lactic-co-glycolic acid) and PLGA/Chitosan Nanoparticles and Chemical Stability of Entrapped Alpha-Tocopherol and Lutein. [Masters Thesis]. Louisiana State University; 2014. Available from: etd-07132014-234534 ; https://digitalcommons.lsu.edu/gradschool_theses/544
University of Texas – Austin
7.
Forciniti, Leandro.
Directing neuronal behavior via polypyrrole-based conductive biomaterials.
Degree: PhD, Chemical Engineering, 2011, University of Texas – Austin
URL: http://hdl.handle.net/2152/ETD-UT-2011-05-3521
► The objective of my thesis is to explore the use of the conducting polymer, polypyrrole, in neural applications. In addition a supplementary aspect of dissertation…
(more)
▼ The objective of my thesis is to explore the use of the conducting polymer, polypyrrole, in neural applications. In addition a supplementary aspect of dissertation will involves understanding the effects of external stimuli on nervous system cells, with the ultimate goal of designing therapeutic systems for nerve regeneration. In normal development and peripheral nervous system repair, nerves encounter naturally occurring chemical, physical, and electrical stimuli. Polypyrrole (PPy) has attracted much attention for use in numerous biomedical applications as it presents chemical, physical and electrical stimuli. In addition, PPy is particularly exciting because the extent by which chemical, physical, and electrical cues are presented to the injured nerve can be easily tailored. Thus, conducting polymers are excellent scaffolds for the exploration of how the cellular components of the nervous system (i.e., Schwann cells and neurons) interact with chemical, topographical, and electrical stimuli.
This dissertation covers three main objectives and is supplemented by two additional topics. The two additional topics explore the effect stimuli present on the conducting polymer PPy have on neural interfaces. These fundamental studies use computational modeling to gain a better understanding of cellular motility on substrates containing different stimuli. Both topics are covered in the appendices of this dissertation. With regards to the three main objectives, I first characterized and optimized the electrochemical synthesis of the conducting polymer, PPy, for Schwann cell biocompatibility. Next, I investigated the effect the application of electrical cues through PPy has on Schwann cell migration. In addition to investigating the effect of the direct electrical current on Schwann cells I also considered the effect that electrical stimulation provided by PPy has on protein adsorption. Finally, I developed a hybrid PPy material that will provide advantageous properties for neural interfaces. Specifically, I describe the development of a polypyrrole:poly-(lactic-co-glycolic) acid blend for neural applications. In summary the three specific objectives covered in my thesis are:
Specific Aim 1: Characterize and optimize the electrochemical synthesis of the conducting polymer, polypyrrole, for Schwann cell biocompatibility
Specific Aim 2: Determine the effect of electrical stimulation on Schwann cell migration
Specific Aim 3: Develop polypyrrole:poly-(lactic-co-glyolic) acid blends for neural engineering applications.
Advisors/Committee Members: Zaman, Muhammad H. (Muhammad Hamid) (advisor), Schmidt, Christine E. (advisor), Sanchez, Isaac C. (committee member), Maynard, Jennifer A. (committee member), Bonnecaze, Roger T. (committee member).
Subjects/Keywords: Polypyrrole; Schwann cells; PLGA; Migration
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APA (6th Edition):
Forciniti, L. (2011). Directing neuronal behavior via polypyrrole-based conductive biomaterials. (Doctoral Dissertation). University of Texas – Austin. Retrieved from http://hdl.handle.net/2152/ETD-UT-2011-05-3521
Chicago Manual of Style (16th Edition):
Forciniti, Leandro. “Directing neuronal behavior via polypyrrole-based conductive biomaterials.” 2011. Doctoral Dissertation, University of Texas – Austin. Accessed March 04, 2021.
http://hdl.handle.net/2152/ETD-UT-2011-05-3521.
MLA Handbook (7th Edition):
Forciniti, Leandro. “Directing neuronal behavior via polypyrrole-based conductive biomaterials.” 2011. Web. 04 Mar 2021.
Vancouver:
Forciniti L. Directing neuronal behavior via polypyrrole-based conductive biomaterials. [Internet] [Doctoral dissertation]. University of Texas – Austin; 2011. [cited 2021 Mar 04].
Available from: http://hdl.handle.net/2152/ETD-UT-2011-05-3521.
Council of Science Editors:
Forciniti L. Directing neuronal behavior via polypyrrole-based conductive biomaterials. [Doctoral Dissertation]. University of Texas – Austin; 2011. Available from: http://hdl.handle.net/2152/ETD-UT-2011-05-3521
8.
Renata Kely de Paulo Moura.
Avaliação in vitro das características de liberação de indometacina a partir de dispositivos oculares implantáveis.
Degree: 2010, Universidade Federal da Paraíba
URL: http://bdtd.biblioteca.ufpb.br/tde_busca/arquivo.php?codArquivo=1226
► A anatomia e fisiologia do olho e suas barreiras protetoras representam um desafio para o desenvolvimento de sistemas de transporte de drogas oftálmicas efetivos. O…
(more)
▼ A anatomia e fisiologia do olho e suas barreiras protetoras representam um desafio para o desenvolvimento de sistemas de transporte de drogas oftálmicas efetivos. O tratamento farmacológico de doenças oculares tem se limitado às formas convencionais de administração que não são satisfatórias para o tratamento de doenças que acometem o segmento posterior do bulbo do olho. O transporte de doses terapêuticas para os tecidos do segmento posterior do olho, que minimize os efeitos colaterais sistêmicos e locais, é o principal objetivo no tratamento de doenças oculares. Visando atingir este objetivo, estudos têm sido feitos no sentido de desenvolver novos sistemas de liberação de fármacos para o olho, entre estes estão os implantes. Esses implantes são preparados a partir de diferentes polímeros, os quais podem ser biodegradáveis ou não biodegradáveis. Os polímeros derivados dos ácidos lático e glicólico têm se revelado bastante promissores devido, principalmente, às suas características de biocompatibilidade e biodegradabilidade. Neste trabalho, dois diferentes implantes de indometacina formulados a partir do copolímero ácido lático / ácido glicólico (
PLGA 50:50) e do polímero derivado do ácido D,L-lático (PLA), através de liofilização da mistura polímero-fármaco, foram fornecidos pela empresa 3T Biopolímeros (São Paulo - SP) e avaliados através de DSC, estudos de liberação in vitro usando aparato de dissolução e estudos de difusão através de esclera de coelho utilizando câmaras de Franz. Os resultados da validação do método CLAE para quantificação da indometacina apresentaram-se dentro dos limites estabelecidos pela legislação brasileira (Resolução RE 899, 2003, ANVISA). As análises de DSC mostraram a ausência aparente de interações químicas e físicas entre o fármaco e os polímeros, sendo sugerido que o desaparecimento do pico de fusão da indometacina tenha ocorrido pela amorfização do fármaco durante o processo de liofilização ou pela diluição do fármaco no polímero. Os estudos preliminares de liberação in vitro demonstraram um perfil trifásico para os implantes formulados com
PLGA e um perfil bifásico para àqueles formulados com PLA. Os implantes formulados com
PLGA promoveram uma liberação mais rápida da indometacina (103,64%) quando comparado com os implantes formulados com PLA (49,9%) durante os trinta dias de experimento. O perfil de liberação da indometacina foi determinado pela velocidade de degradação dos polímeros, que também determinou a difusão escleral da indometacina a partir do
PLGA e do PLA (1,7 x 10-5 cm/s e 0,24 x 10-5 cm/s, respectivamente). Os estudos de difusão escleral mostraram que a esclera de coelho é permeável à indometacina e a microscopia de luz polarizada mostrou que a estrutura das fibras colágenas da esclera não foram significativamente alteradas durante o estudo de difusão nas câmaras de Franz. Os sistemas de liberação de fármacos avaliados foram capazes de liberar a indometacina de forma prolongada, servindo como um modelo para formulação de implantes de indometacina que podem ser…
Advisors/Committee Members: Eduardo de Jesus Oliveira.
Subjects/Keywords: PLA; indomethacin; Ocular implants; Implantes oculares; PLGA; Indometacina; FARMACOLOGIA; PLA; PLGA
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APA (6th Edition):
Moura, R. K. d. P. (2010). Avaliação in vitro das características de liberação de indometacina a partir de dispositivos oculares implantáveis. (Thesis). Universidade Federal da Paraíba. Retrieved from http://bdtd.biblioteca.ufpb.br/tde_busca/arquivo.php?codArquivo=1226
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Moura, Renata Kely de Paulo. “Avaliação in vitro das características de liberação de indometacina a partir de dispositivos oculares implantáveis.” 2010. Thesis, Universidade Federal da Paraíba. Accessed March 04, 2021.
http://bdtd.biblioteca.ufpb.br/tde_busca/arquivo.php?codArquivo=1226.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Moura, Renata Kely de Paulo. “Avaliação in vitro das características de liberação de indometacina a partir de dispositivos oculares implantáveis.” 2010. Web. 04 Mar 2021.
Vancouver:
Moura RKdP. Avaliação in vitro das características de liberação de indometacina a partir de dispositivos oculares implantáveis. [Internet] [Thesis]. Universidade Federal da Paraíba; 2010. [cited 2021 Mar 04].
Available from: http://bdtd.biblioteca.ufpb.br/tde_busca/arquivo.php?codArquivo=1226.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Moura RKdP. Avaliação in vitro das características de liberação de indometacina a partir de dispositivos oculares implantáveis. [Thesis]. Universidade Federal da Paraíba; 2010. Available from: http://bdtd.biblioteca.ufpb.br/tde_busca/arquivo.php?codArquivo=1226
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
9.
Courant, Thomas.
Nanoparticules incorporant des complexes inorganiques à visée diagnostique : Metal complexes-loaded nanoparticles for diagnostic and medical imaging.
Degree: Docteur es, Chimie, 2011, Reims
URL: http://www.theses.fr/2011REIMS005
► L’objectif poursuivi au cours de ce travail est l’encapsulation de complexes métalliques au sein de nanoparticules biocompatibles, et ce pour des visées diagnostiques. Dans ce…
(more)
▼ L’objectif poursuivi au cours de ce travail est l’encapsulation de complexes métalliques au sein de nanoparticules biocompatibles, et ce pour des visées diagnostiques. Dans ce but, un protocole de double émulsion-diffusion de solvant Wi/O/We, n’utilisant que des composés biocompatibles, a été mis au point et optimisé pour obtenir, de façon quantitative et reproductible, des nanoparticules de PLGA de diamètre compatible avec une injection par voie parentérale. Cette formulation a été employée avec succès pour l’encapsulation de complexes modèles de Cu(II). Les formulations optimales permettent d’obtenir des nanoparticules possédant des diamètres hydrodynamiques moyens inférieurs à 200 nm avec des efficacités d’encapsulation entre 20 et 25 %. L’utilisation de cette formulation pour l’encapsulation de chélates de gadolinium ne permet pas d’obtenir des rendements d’encapsulation satisfaisants. La modification du protocole vers une méthodologie Wi/O1/O2ne permet pas d’améliorer l’encapsulation et dénote l’absence d’affinités entre le polymère hydrophobe et les complexes hydrophiles. L’utilisation de nanoparticules composées d’une matrice hydrophile permet d’obtenir des taux de charges nettement supérieurs. Ceci conforte l’hypothèse selon laquelle les interactions entre le complexe et la matrice des nanoparticules jouent un rôle crucial pour l’encapsulation.
The goal of this work was to encapsulate metal complexes into biocompatible nanoparticles for diagnostics. To reach this purpose, a double emulsion-solvent diffusion Wi/O/We technique was optimized, using only biocompatible compounds. It allowed the obtention of PLGA nanoparticles that are compatible with parenteral injections in a reproducible and quantitative way. This formulation was successfully applied to encapsulate model Cu(II)complexes. Optimal formulations showed mean diameters below 200 nm with encapsulation yields in the 20-25 % range. The use of this formulation for gadolinium chelates did not lead to satisfactory encapsulation yields. Thereafter, a Wi/O1/O2 methodology was developed but could not allow to raise the encapsulation efficiencies. This point showed the lack of affinity between the hydrophobic polymer and the hydrophilic chelates. The use of nanoparticles made of an hydrophilic matrix showed a ten-fold increase in the drug loading efficiency. This confirms the hypothesis in which interactions between chelates and nanoparticle matrices play a crucial role for encapsulation.
Advisors/Committee Members: Chuburu, Françoise (thesis director), Andry, Marie-Christine (thesis director).
Subjects/Keywords: Encapsulation; Gadolinium; Agents de contraste.; PLGA; Encapsulation; Gadolinium; Contrast agents.; PLGA
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APA ·
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Manager
APA (6th Edition):
Courant, T. (2011). Nanoparticules incorporant des complexes inorganiques à visée diagnostique : Metal complexes-loaded nanoparticles for diagnostic and medical imaging. (Doctoral Dissertation). Reims. Retrieved from http://www.theses.fr/2011REIMS005
Chicago Manual of Style (16th Edition):
Courant, Thomas. “Nanoparticules incorporant des complexes inorganiques à visée diagnostique : Metal complexes-loaded nanoparticles for diagnostic and medical imaging.” 2011. Doctoral Dissertation, Reims. Accessed March 04, 2021.
http://www.theses.fr/2011REIMS005.
MLA Handbook (7th Edition):
Courant, Thomas. “Nanoparticules incorporant des complexes inorganiques à visée diagnostique : Metal complexes-loaded nanoparticles for diagnostic and medical imaging.” 2011. Web. 04 Mar 2021.
Vancouver:
Courant T. Nanoparticules incorporant des complexes inorganiques à visée diagnostique : Metal complexes-loaded nanoparticles for diagnostic and medical imaging. [Internet] [Doctoral dissertation]. Reims; 2011. [cited 2021 Mar 04].
Available from: http://www.theses.fr/2011REIMS005.
Council of Science Editors:
Courant T. Nanoparticules incorporant des complexes inorganiques à visée diagnostique : Metal complexes-loaded nanoparticles for diagnostic and medical imaging. [Doctoral Dissertation]. Reims; 2011. Available from: http://www.theses.fr/2011REIMS005
University of California – Irvine
10.
Rapier, Crystal Eunique.
DROPLET BASED MICROFLUIDICS FOR THE DEVELOPMENT OF A NOVEL BIOABSORBABLE IMMUNOMODULATORY MATERIAL WITH APPLICATIONS IN THE IMPLANTABLE MEDICAL DEVICE FIELD.
Degree: Biomedical Engineering, 2016, University of California – Irvine
URL: http://www.escholarship.org/uc/item/30d2s9kn
► Inflammation and the foreign body response (FBR) pose a major problem to the successful function of essentially “foreign” implanted medical devices such as vascular stents,…
(more)
▼ Inflammation and the foreign body response (FBR) pose a major problem to the successful function of essentially “foreign” implanted medical devices such as vascular stents, heart valves, hip replacements, knee replacements, etc. Conventional implants are permanent and usually made from surgical grade stainless steel metal. Despite the efficacy of conventional materials, there is a need for non-permanent implant solutions. Poly(lactic-co-glycolic acid) (PLGA) is a bioabsorbable material that is nontoxic and FDA approved for implantation. However, bioabsorbable materials such as PLGA can induce a distinct acute inflammatory immune response. To date, a highly successful solution to the prevention of the FBR to implanted materials has not been created. To circumvent this crucial problem, this dissertation describes the creation of novel a biomaterial by combining the bioabsorbable property of PLGA with the anti-inflammatory property of an immunomodulatory protein. This protein allows the biomaterial to be recognized as part of the body.In this work, droplet based microfluidics was used as a platform to generate microspheres and microcapsules using single and double emulsion methods, respectively. The immunomodulatory protein is conjugated to the surface of the microspheres or encapsulated within microcapsules. Herein, microparticles were assessed to see whether the immunomodulatory protein can tolerize the host immune system to the foreign material and reduce/inhibit an inflammatory response to PLGA.
Subjects/Keywords: Biomedical engineering; Immunology; Immunomodulatory; Microfluidics; PLGA
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APA (6th Edition):
Rapier, C. E. (2016). DROPLET BASED MICROFLUIDICS FOR THE DEVELOPMENT OF A NOVEL BIOABSORBABLE IMMUNOMODULATORY MATERIAL WITH APPLICATIONS IN THE IMPLANTABLE MEDICAL DEVICE FIELD. (Thesis). University of California – Irvine. Retrieved from http://www.escholarship.org/uc/item/30d2s9kn
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Rapier, Crystal Eunique. “DROPLET BASED MICROFLUIDICS FOR THE DEVELOPMENT OF A NOVEL BIOABSORBABLE IMMUNOMODULATORY MATERIAL WITH APPLICATIONS IN THE IMPLANTABLE MEDICAL DEVICE FIELD.” 2016. Thesis, University of California – Irvine. Accessed March 04, 2021.
http://www.escholarship.org/uc/item/30d2s9kn.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Rapier, Crystal Eunique. “DROPLET BASED MICROFLUIDICS FOR THE DEVELOPMENT OF A NOVEL BIOABSORBABLE IMMUNOMODULATORY MATERIAL WITH APPLICATIONS IN THE IMPLANTABLE MEDICAL DEVICE FIELD.” 2016. Web. 04 Mar 2021.
Vancouver:
Rapier CE. DROPLET BASED MICROFLUIDICS FOR THE DEVELOPMENT OF A NOVEL BIOABSORBABLE IMMUNOMODULATORY MATERIAL WITH APPLICATIONS IN THE IMPLANTABLE MEDICAL DEVICE FIELD. [Internet] [Thesis]. University of California – Irvine; 2016. [cited 2021 Mar 04].
Available from: http://www.escholarship.org/uc/item/30d2s9kn.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Rapier CE. DROPLET BASED MICROFLUIDICS FOR THE DEVELOPMENT OF A NOVEL BIOABSORBABLE IMMUNOMODULATORY MATERIAL WITH APPLICATIONS IN THE IMPLANTABLE MEDICAL DEVICE FIELD. [Thesis]. University of California – Irvine; 2016. Available from: http://www.escholarship.org/uc/item/30d2s9kn
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
University of Rochester
11.
Tseng, Kuang-Ching Chris (1981 - ); Elfar, John C.
The potential therapeutic approaches to improve
peripheral nerve functional recovery from injury.
Degree: PhD, 2015, University of Rochester
URL: http://hdl.handle.net/1802/29718
► Traumatic peripheral nerve crush injuries contribute a great deal of morbidity in patients and little improvement has been made in the treatment of these injuries…
(more)
▼ Traumatic peripheral nerve crush injuries
contribute a great deal of morbidity in patients and little
improvement has been made in the treatment of these injuries in
many years. Diagnosis and treatment of traumatic peripheral nerve
damage suffer from a lack of means of accurately diagnosing the
reasons for loss of nerve function and a lack of means of enhancing
the time course of repair in injuries in which nerve function is
lost for reasons other than axonal transection.
</br>
In the current work, we demonstrate that
administration of erythropoietin (EPO) shortens the time course of
recovery after experimental crush injury to the mouse sciatic
nerve. We found that systemic EPO administration maintained more
myelinated axons at the site of injury in vivo following sciatic
nerve crush. In vitro, EPO treatment promotes myelin formation and
protects myelin from the effects of nitric oxide exposure in
co-cultures of Schwann cells and dorsal root ganglion (DRG)
neurons. These results suggested local EPO application might also
be effective in promoting functional recovery, which would avoid
potential systemic side effects of systemic EPO administration. We
therefore developed a novel local treatment for peripheral nerve
injury of EPO delivered in a fibrin glue matrix at the site of
injury. Local delivery was as effective as systemic EPO
administration, and thus may provide a potentially useful approach
to enhancing recovery in peripheral nerve injuries.
</br>
We also demonstrate that a single
administration of 4-aminopyridine (4-AP), which enables impulse
conduction in demyelinated axons, transiently restores function and
allows identification of injuries in which axons are intact but not
functional. We further demonstrate that repeated 4-AP
administration markedly improves the time course of recovery from
crush injuries in the peripheral nerve as determined by behavioral
and electophysiological analyses. Equal or greater efficacy is
provided by localized delivery of slow-release formulations of 4-AP
at the lesion site. In addition, we provide biochemical and
ultrastructural evidence to show that repeated systemic 4-AP
delivery and localized slow-release delivery
enhance remyelination
after injury. Thus, we provide a single therapeutic approach that
addresses critical issues in diagnosing peripheral nerve injury and
enhancing repair, and provides the first pharmacological approach
to enhancing myelin repair. As 4-AP is already used in the clinic
for treatment of multiple
sclerosis, our approaches are highly
suitable for rapid translation from the laboratory to clinical
analysis.
Subjects/Keywords: 4-Aminopyridine; Erythropoietin; PLGA; Peripheral nerve injury
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APA (6th Edition):
Tseng, Kuang-Ching Chris (1981 - ); Elfar, J. C. (2015). The potential therapeutic approaches to improve
peripheral nerve functional recovery from injury. (Doctoral Dissertation). University of Rochester. Retrieved from http://hdl.handle.net/1802/29718
Chicago Manual of Style (16th Edition):
Tseng, Kuang-Ching Chris (1981 - ); Elfar, John C. “The potential therapeutic approaches to improve
peripheral nerve functional recovery from injury.” 2015. Doctoral Dissertation, University of Rochester. Accessed March 04, 2021.
http://hdl.handle.net/1802/29718.
MLA Handbook (7th Edition):
Tseng, Kuang-Ching Chris (1981 - ); Elfar, John C. “The potential therapeutic approaches to improve
peripheral nerve functional recovery from injury.” 2015. Web. 04 Mar 2021.
Vancouver:
Tseng, Kuang-Ching Chris (1981 - ); Elfar JC. The potential therapeutic approaches to improve
peripheral nerve functional recovery from injury. [Internet] [Doctoral dissertation]. University of Rochester; 2015. [cited 2021 Mar 04].
Available from: http://hdl.handle.net/1802/29718.
Council of Science Editors:
Tseng, Kuang-Ching Chris (1981 - ); Elfar JC. The potential therapeutic approaches to improve
peripheral nerve functional recovery from injury. [Doctoral Dissertation]. University of Rochester; 2015. Available from: http://hdl.handle.net/1802/29718
12.
Yang, Jessica Pei-Wen.
ELECTROSPUN PLGA MICROFIBERS FOR LOCALIZED DELIVERY OF SMALL MOLECULES TO INDUCE BROWN ADIPOGENESIS.
Degree: 2014, Johns Hopkins University
URL: http://jhir.library.jhu.edu/handle/1774.2/37102
► Obesity and its related disorders have been on the rise in the last few decades. Current prescribed treatments range from diet and physical activity for…
(more)
▼ Obesity and its related disorders have been on the rise in the last few decades. Current prescribed treatments range from diet and physical activity for low risk patients to pharmacological intervention and surgical procedures for higher risk patients. In the past few years, with the discovery of its presence in adult humans, brown adipose tissue has emerged as an interesting tool for reducing the risk of developing obesity or improving weight reduction in overweight or obese individuals. This study aims to create a system to deliver drugs specifically to white fat pads to increase brown fat differentiation, thus increase overall energy expenditure and improve energy metabolism in obesity and other metabolic disorders. The designed model system encapsulates rosiglitazone, a small molecule anti-diabetic drug, within electrospun poly(D,L-lactide-co-glycolide) (
PLGA) microfibers. Drug release is sustained over a period of 35 days from microfibers with 0.5% w/w
rosiglitazone loaded. In vitro experiments with the microfibers in a Transwell system demonstrate that 0.5% rosiglitazone was an optimal concentration for stimulating brown adipocyte differentiation. Furthermore, the rosiglitazone released from the microfibers was able to stimulate differentiation of white adipose-derived stem cells towards a brown adipocyte lineage. These results provide promising steps towards developing a therapy to create a localized depot of brown fat in situ that can be easily administered in the clinic to supplement current obesity treatment and prevention techniques.
Advisors/Committee Members: Yarema, Kevin (advisor).
Subjects/Keywords: Brown fat; localized delivery; rosiglitazone; PLGA
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APA (6th Edition):
Yang, J. P. (2014). ELECTROSPUN PLGA MICROFIBERS FOR LOCALIZED DELIVERY OF SMALL MOLECULES TO INDUCE BROWN ADIPOGENESIS. (Thesis). Johns Hopkins University. Retrieved from http://jhir.library.jhu.edu/handle/1774.2/37102
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Yang, Jessica Pei-Wen. “ELECTROSPUN PLGA MICROFIBERS FOR LOCALIZED DELIVERY OF SMALL MOLECULES TO INDUCE BROWN ADIPOGENESIS.” 2014. Thesis, Johns Hopkins University. Accessed March 04, 2021.
http://jhir.library.jhu.edu/handle/1774.2/37102.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Yang, Jessica Pei-Wen. “ELECTROSPUN PLGA MICROFIBERS FOR LOCALIZED DELIVERY OF SMALL MOLECULES TO INDUCE BROWN ADIPOGENESIS.” 2014. Web. 04 Mar 2021.
Vancouver:
Yang JP. ELECTROSPUN PLGA MICROFIBERS FOR LOCALIZED DELIVERY OF SMALL MOLECULES TO INDUCE BROWN ADIPOGENESIS. [Internet] [Thesis]. Johns Hopkins University; 2014. [cited 2021 Mar 04].
Available from: http://jhir.library.jhu.edu/handle/1774.2/37102.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Yang JP. ELECTROSPUN PLGA MICROFIBERS FOR LOCALIZED DELIVERY OF SMALL MOLECULES TO INDUCE BROWN ADIPOGENESIS. [Thesis]. Johns Hopkins University; 2014. Available from: http://jhir.library.jhu.edu/handle/1774.2/37102
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Penn State University
13.
Fattahi, Pouria.
Characterization Of The Size, Shape, And Drug Encapsulation Efficiency Of Plga Microcapsules Produced Via Electrojetting For Anticancer Agent Delivery.
Degree: 2013, Penn State University
URL: https://submit-etda.libraries.psu.edu/catalog/19927
► Despite significant progress in the development of new chemotherapeutic agents and drug delivery methods for brain tumors, malignant gliomas (high grade brain tumor) remains deadly…
(more)
▼ Despite significant progress in the development of new chemotherapeutic agents and drug delivery methods for brain tumors, malignant gliomas (high grade brain tumor) remains deadly with a median survival period of only about a year. The high dosage of chemotherapeutic agents required for penetration through the blood brain barrier during chemotherapy not only kills cancer cells but also damages healthy tissues and causes adverse side effects. Hence, a major unmet challenge in the treatment of malignant gliomas is the development of effective and targeted local delivery of chemotherapeutic agents at the cellular level. Drug-loaded biodegradable microcapsules with high drug encapsulation efficiency and controlled shape and size are attractive candidates for a more precise control of anticancer agent delivery at the tumor sites.
Here, I report the results of a systematic study of the size, shape, and drug release profiles of Poly(lactic-co glycolic) (
PLGA) microcapsules produced and loaded with the anticancer agent 1,3-bis(2-chloroethyl)-1-nitrosourea (BCNU) using an electrojetting technique. I report production of BCNU-loaded
PLGA microcapsules in the form of flattened microspheres, microspheres, and microfibers with significantly (1) higher drug encapsulation efficiency, (2) more tunable drug loading capacity, and (3) narrower size distribution than those generated using other encapsulation methods. To prepare BCNU-
PLGA solutions with
PLGA concentrations ranging from 1 to 10 wt%, BCNU/
PLGA mixtures (5 to 25 wt% with respect to
PLGA) dissolved in chloroform were electrojetted on gold substrates. I quantified the shape and size distribution of BCNU-loaded
PLGA microcapsules as a function of the polymer concentration and flow rate used during electrojetting, and characterized drug release profiles for microcapsules of three different morphologies: flattened microspheres, microspheres, and microfibers. Flattened microspheres were produced from 1 and 2 wt%
PLGA solutions, while microspheres and microfibers were formed as the
PLGA concentration increased to 3-5 wt% and 10% wt%, respectively. I investigated the effect of flow rate (i.e. 0.25, 0.5 and 1 mLh-1) on the size and monodispersity of BCNU-loaded
PLGA microcapsules. Microcapsules of 1 wt%
PLGA, 4 wt%
PLGA, and 10 wt%
PLGA formed at a flow rate of Q= 0.25 mLh-1 had narrower size distributions (CV= 4.6%, CV= 4.5%, and CV= 4.8%, respectively). A commonly accepted definition of monodispersity is CV < 5%. I also measured drug release profiles and developed mathematical models to estimate the effective diffusion coefficient of BCNU in different
PLGA microcapsules. The BCNU release profiles for all three microcapsule morphologies were found to be in good agreement with model predictions for drug release as a result of drug diffusion and degradation of
PLGA microcapsules.
Advisors/Committee Members: Mohammad Reza Abidian, Thesis Advisor/Co-Advisor.
Subjects/Keywords: BCNU; Drug delivery; PLGA; Mathematical modeling
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APA (6th Edition):
Fattahi, P. (2013). Characterization Of The Size, Shape, And Drug Encapsulation Efficiency Of Plga Microcapsules Produced Via Electrojetting For Anticancer Agent Delivery. (Thesis). Penn State University. Retrieved from https://submit-etda.libraries.psu.edu/catalog/19927
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Fattahi, Pouria. “Characterization Of The Size, Shape, And Drug Encapsulation Efficiency Of Plga Microcapsules Produced Via Electrojetting For Anticancer Agent Delivery.” 2013. Thesis, Penn State University. Accessed March 04, 2021.
https://submit-etda.libraries.psu.edu/catalog/19927.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Fattahi, Pouria. “Characterization Of The Size, Shape, And Drug Encapsulation Efficiency Of Plga Microcapsules Produced Via Electrojetting For Anticancer Agent Delivery.” 2013. Web. 04 Mar 2021.
Vancouver:
Fattahi P. Characterization Of The Size, Shape, And Drug Encapsulation Efficiency Of Plga Microcapsules Produced Via Electrojetting For Anticancer Agent Delivery. [Internet] [Thesis]. Penn State University; 2013. [cited 2021 Mar 04].
Available from: https://submit-etda.libraries.psu.edu/catalog/19927.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Fattahi P. Characterization Of The Size, Shape, And Drug Encapsulation Efficiency Of Plga Microcapsules Produced Via Electrojetting For Anticancer Agent Delivery. [Thesis]. Penn State University; 2013. Available from: https://submit-etda.libraries.psu.edu/catalog/19927
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Iowa State University
14.
Chen, Yunqing.
An implantable sustained-release chemotherapy delivery system for the treatment of breast cancer.
Degree: 2015, Iowa State University
URL: https://lib.dr.iastate.edu/etd/14784
► The influence of chitosan on the degradation characteristics of paclitaxel-loaded Poly (Lactic-co-Glycolic Acid) (PLGA) rod-shaped implants was investigated and modeled. The implant was designed for…
(more)
▼ The influence of chitosan on the degradation characteristics of paclitaxel-loaded Poly (Lactic-co-Glycolic Acid) (PLGA) rod-shaped implants was investigated and modeled. The implant was designed for sustained release of the hydrophobic chemotherapeutic paclitaxel (PTX) intramuscularly or subcutaneously. In this study, integration of PTX and PLGA was achieved via a solvent evaporation method resulting in a solid dispersion of the substances. To customize degradation of the implants and secure delivery of high doses of PTX, chitosan and the PLGA-PTX blend were mixed in a 30:70 mass ratio. Cryomilling was utilized to create the chitosan-PLGA-PTX mixture, due to its proven effectiveness in producing homogenous blends. Implants were then fabricated into rods by injection molding and characterized in terms of content uniformity, morphology, and thermal property. The integrity of the blends was ascertained via x-ray diffraction, while miscibility between the drug and excipients was established by differential scanning calorimetry. In vitro drug release was studied in a phosphate buffer of pH 7.4 and measured by ultraviolet-visible spectrophotometry (UV-Vis). Meanwhile, the degradation rate was determined by quantifying mass loss at various points in 30 days. This study revealed the chitosan blended PTX-loaded PLGA implant possesses a longer, yet steadier, sustained drug release behavior than the PTX-loaded PLGA implant. The results suggest that introducing chitosan into PLGA implants through this fabrication method could be integrated to regulate and control the degradation rate of PLGA implants.
Subjects/Keywords: Industrial Engineering; Chitosan; Cryomilling; PLGA; Engineering
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APA ·
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MLA ·
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CSE |
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APA (6th Edition):
Chen, Y. (2015). An implantable sustained-release chemotherapy delivery system for the treatment of breast cancer. (Thesis). Iowa State University. Retrieved from https://lib.dr.iastate.edu/etd/14784
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Chen, Yunqing. “An implantable sustained-release chemotherapy delivery system for the treatment of breast cancer.” 2015. Thesis, Iowa State University. Accessed March 04, 2021.
https://lib.dr.iastate.edu/etd/14784.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Chen, Yunqing. “An implantable sustained-release chemotherapy delivery system for the treatment of breast cancer.” 2015. Web. 04 Mar 2021.
Vancouver:
Chen Y. An implantable sustained-release chemotherapy delivery system for the treatment of breast cancer. [Internet] [Thesis]. Iowa State University; 2015. [cited 2021 Mar 04].
Available from: https://lib.dr.iastate.edu/etd/14784.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Chen Y. An implantable sustained-release chemotherapy delivery system for the treatment of breast cancer. [Thesis]. Iowa State University; 2015. Available from: https://lib.dr.iastate.edu/etd/14784
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Brno University of Technology
15.
Oborná, Jana.
Řízené uvolňování léčiv z biodegradabilních hydrogelů.: Controlled Drug Release from Biodegradable Hydrogels.
Degree: 2019, Brno University of Technology
URL: http://hdl.handle.net/11012/84164
► This dissertation is focused on the controlled release of drugs from a biodegradable amphiphilic hydrogel based on hydrophobic poly(lactic acid), poly(glycolic acid) and hydrophilic poly(ethylene…
(more)
▼ This dissertation is focused on the controlled release of drugs from a biodegradable amphiphilic hydrogel based on hydrophobic poly(lactic acid), poly(glycolic acid) and hydrophilic poly(ethylene glycol) (
PLGA-PEG-
PLGA, ABA) and its modification with itaconic anhydride (ITA). The resulting ,-itaconyl(
PLGA-PEG-
PLGA) copolymer is referred to as ITA/
PLGA-PEG-
PLGA/ITA or ITA/ABA/ITA. Itaconic acid provides reactive double bonds and a functional carboxyl group at the ends of the
PLGA-PEG-
PLGA copolymer chain, thereby rendering the modified ITA/ABA/ITA copolymer less hydrophobic and offering the possibility of forming a carrier for hydrophilic drug substances. These functional copolymers are thermosensitive and change in the external environment (e.g. temperature) causes a sol-gel phase transition due to the formation of micellar structure. The bioactive substances can thus be mixed with a copolymer which is in a low viscous phase (sol phase) and subsequently the mixture can be injected into patient's body at the target site where it forms a gel at 37 °C. This hydrogel becomes a drug depot, which gradually releases the active substance. Prediction of the substance’s release profile from the hydrogel is an effective tool to determine the frequency of administration, potentially enhancing efficacy, and assessment of side effects associated with dosing. The analgesic paracetamol and the sulfonamide antibiotic sulfathiazole were used as model drugs, representing hydrophilic and hydrophobic substances, respectively. The active substances had a significant effect on the resulting hydrogel stiffness. Type of solvent, incubation medium and nanohydroxyapatite also influenced on the gel stiffness and subsequent stability of the hydrogel-drug system. Controlled release of drugs took place in simulated conditions of the human body. Verification of Korsmeyer-Peppas (KP) drug-release model is also discussed in this thesis. The KP model was found suitable for simulating the release of sulfathiazole from ABA and ITA/ABA/ITA hydrogels. On the contrary, the performance of KP model was not suitable for describing the release of paracetamol from the ABA hydrogels. Therefore, a new regression model suitable for both buffered simulated media and water has been proposed. The proposed model fitted better the release of both sulfathiazole and paracetamol from composite material prepared from ABA hydrogel and nanohydroxyapatite.
Advisors/Committee Members: Vávrová, Milada (advisor), Chýlková, Jaromíra (referee), Kráčmar, Stanislav (referee), Kučerík, Jiří (referee).
Subjects/Keywords: hydrogel; PLGA-PEG-PLGA kopolymer; profil uvolňování léčiv; léčiva; Korsmeyer-Peppasův model; hydrogel; PLGA-PEG-PLGA copolymer; drug release profile; drugs; Korsmeyer-Peppas model
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APA ·
Chicago ·
MLA ·
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CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Oborná, J. (2019). Řízené uvolňování léčiv z biodegradabilních hydrogelů.: Controlled Drug Release from Biodegradable Hydrogels. (Thesis). Brno University of Technology. Retrieved from http://hdl.handle.net/11012/84164
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Oborná, Jana. “Řízené uvolňování léčiv z biodegradabilních hydrogelů.: Controlled Drug Release from Biodegradable Hydrogels.” 2019. Thesis, Brno University of Technology. Accessed March 04, 2021.
http://hdl.handle.net/11012/84164.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Oborná, Jana. “Řízené uvolňování léčiv z biodegradabilních hydrogelů.: Controlled Drug Release from Biodegradable Hydrogels.” 2019. Web. 04 Mar 2021.
Vancouver:
Oborná J. Řízené uvolňování léčiv z biodegradabilních hydrogelů.: Controlled Drug Release from Biodegradable Hydrogels. [Internet] [Thesis]. Brno University of Technology; 2019. [cited 2021 Mar 04].
Available from: http://hdl.handle.net/11012/84164.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Oborná J. Řízené uvolňování léčiv z biodegradabilních hydrogelů.: Controlled Drug Release from Biodegradable Hydrogels. [Thesis]. Brno University of Technology; 2019. Available from: http://hdl.handle.net/11012/84164
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Duquesne University
16.
Wang, Yiwei.
Macromolecule Loading on PLGA Particles through Surface Adsorption.
Degree: MS, Pharmaceutics, 2018, Duquesne University
URL: https://dsc.duq.edu/etd/1488
► The advent of biotherapeutics have impacted the ways in which diseases are treated. In many instances development of recombinant proteins and oligonucleotides into therapeutics…
(more)
▼ The advent of biotherapeutics have impacted the ways in which diseases are treated. In many instances development of recombinant proteins and oligonucleotides into therapeutics are slowed by poor stability of the candidate drugs. Progress has been made in using polymeric particles as carriers of macromolecule drugs. Historically the method is to encapsulate biological drugs into spherical polymeric matrices. Owing to its biocompatible and biodegradable nature, poly (d,l-lactic-co-glycolic acid) (
PLGA) has been used extensively in formulating biological drugs into nano- and micro-sized particles. The development of
PLGA biologic formulations, however, has been hampered by matrix acidification; the hydrolysis of the polymer generates non-diffusive acid fragments by which the interior pH can be driven to as low as 2, a condition unfavorable for proteins and oligonucleotides to remain intact. In addition, loading of proteins through encapsulation can lead to substantial loss of activity because of homogenization or sonication-induced denaturation. To circumvent these limitations yet still take advantage of the proven safety of
PLGA in humans, I proposed to load macromolecules onto the surface of
PLGA particles functionalized with biotin (“
PLGA-biotin”).
The specific binding between avidin and biotin (or desthiobiotin) was employed to enable surface adsorption. The pair has the strongest known non-covalent affinity, an interaction that is relatively insensitive to temperature and pH.
PLGA-biotin particles were prepared by co-emulsifying the lipid DSPE-PEG-biotin with
PLGA into a matrix. The tetravalent feature of avidin was used to bridge biotinylated proteins or oligonucleotides onto
PLGA- biotin particles. The hypothesis of this project is that solvent-accessible biotin molecules are displayed on
PLGA-biotin particles. The rationale for my hypothesis is that the co- emulsification of the biotinylated lipid DSPE-PEG-biotin and the polymer matrix
PLGA has the potential to generate
PLGA particles functionalized with biotin molecules embedded on the surface. As a result, the interaction between biotin and avidin can be utilized to load macromolecules onto the surface of these modified
PLGA particles through adsorption, circumventing the challenges to the stability and bioactivity of the biological drugs caused by the encapsulation process and the acidic environment inside
PLGA particles during the degradation of the polymer matrix. Two specific aims were carried out. In aim 1, the presence of biotin in
PLGA-biotin particles was verified and the physical attributes of the particles, namely particle size and zeta potential, were characterized. Spectroscopic and biochemical studies indicated the presence of biotin on the particle surface. In aim 2, the amount of biotin accessible on
PLGA-biotin particles was quantified. Taken together, the data generated in this thesis support the notion that surface adsorption is a general method for loading macromolecules onto
PLGA particles. The work presented is a step toward a…
Advisors/Committee Members: Wilson Meng, Ellen Gawalt, Devika Soundara Manickam.
Subjects/Keywords: PLGA; avidin; biotin; adsorption; Pharmaceutical Preparations
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APA ·
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MLA ·
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CSE |
Export
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APA (6th Edition):
Wang, Y. (2018). Macromolecule Loading on PLGA Particles through Surface Adsorption. (Thesis). Duquesne University. Retrieved from https://dsc.duq.edu/etd/1488
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Wang, Yiwei. “Macromolecule Loading on PLGA Particles through Surface Adsorption.” 2018. Thesis, Duquesne University. Accessed March 04, 2021.
https://dsc.duq.edu/etd/1488.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Wang, Yiwei. “Macromolecule Loading on PLGA Particles through Surface Adsorption.” 2018. Web. 04 Mar 2021.
Vancouver:
Wang Y. Macromolecule Loading on PLGA Particles through Surface Adsorption. [Internet] [Thesis]. Duquesne University; 2018. [cited 2021 Mar 04].
Available from: https://dsc.duq.edu/etd/1488.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Wang Y. Macromolecule Loading on PLGA Particles through Surface Adsorption. [Thesis]. Duquesne University; 2018. Available from: https://dsc.duq.edu/etd/1488
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
University of Sydney
17.
Ashhurt, Anneliese.
Pulmonary vaccines and immune responses to control tuberculosis
.
Degree: 2017, University of Sydney
URL: http://hdl.handle.net/2123/17207
► Tuberculosis remains a staggering burden on global health, despite considerable research efforts toward preventative strategies. Subunit vaccines offer potential as safe generators of protective immunity,…
(more)
▼ Tuberculosis remains a staggering burden on global health, despite considerable research efforts toward preventative strategies. Subunit vaccines offer potential as safe generators of protective immunity, and there is growing interest in exploring delivery by the pulmonary route to enhance immunogenicity. In this study, novel subunit vaccines were designed and produced utilising a variety of adjuvants and protein antigens from Mycobacterium tuberculosis (Mtb), with a focus on formulation for pulmonary delivery. The immunogenicity and protective efficacy of these vaccines was assessed in a murine model of Mtb infection. First, the novel TLR2-ligand adjuvant, Lipokel®, was non-covalently conjugated to proteins from Mtb and formulated as dry powders for inhalation, delivering antigen into the lungs in a self-adjuvanting context. Second, immunodominant peptide epitopes from Mtb were covalently conjugated to Pam2Cys to provide stable lipopeptide vaccines. Third, strategies for covalent conjugation of full length recombinant proteins from Mtb to Pam2Cys are shown. Finally, the novel polysaccharide adjuvant AdvaxTM was tested in combination with fusion proteins as a pulmonary vaccine and this resulted in highly significant protection from Mtb challenge. A different approach used biodegradable poly(lactic-co-glycolic acid) particles as a carrier for MPT83 and adjuvants, formulated for pulmonary delivery. The protective efficacy of these particulate vaccines against Mtb was compared to the effect of DDA-liposome based vaccine formulations. Chemokine receptors play an important role in the recruitment of T-cells to the lungs following immunisation or infection. Therefore the role of CXCR6 was examined in early and chronic Mtb infection, and this was compared to the CD4+ T-cell responses induced by a recombinant influenza A virus vaccine. Paradoxically, CXCR6 deficiency increased protection against these pathogens and resulted in improved control of Mtb infection. These studies emphasise the critical nature of adjuvant choice and administration route on the efficacy of TB vaccines, and the novel effective vaccines developed in this study represent a new approach to prevent TB.
Subjects/Keywords: Tuberculosis;
Pulmonary vaccines;
Pam2Cys;
Advax;
PLGA;
CXCR6
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APA ·
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Export
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Manager
APA (6th Edition):
Ashhurt, A. (2017). Pulmonary vaccines and immune responses to control tuberculosis
. (Thesis). University of Sydney. Retrieved from http://hdl.handle.net/2123/17207
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Ashhurt, Anneliese. “Pulmonary vaccines and immune responses to control tuberculosis
.” 2017. Thesis, University of Sydney. Accessed March 04, 2021.
http://hdl.handle.net/2123/17207.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Ashhurt, Anneliese. “Pulmonary vaccines and immune responses to control tuberculosis
.” 2017. Web. 04 Mar 2021.
Vancouver:
Ashhurt A. Pulmonary vaccines and immune responses to control tuberculosis
. [Internet] [Thesis]. University of Sydney; 2017. [cited 2021 Mar 04].
Available from: http://hdl.handle.net/2123/17207.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Ashhurt A. Pulmonary vaccines and immune responses to control tuberculosis
. [Thesis]. University of Sydney; 2017. Available from: http://hdl.handle.net/2123/17207
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
18.
Melo, Carina Guimarães de Souza.
Enxerto sintético e biológico, com e sem preenchimento de veia jugular externa, no reparo de nervo periférico em ratos.
Degree: Mestrado, Estomatologia e Biologia Oral, 2011, University of São Paulo
URL: http://www.teses.usp.br/teses/disponiveis/25/25149/tde-24082011-103123/
;
► Apesar do desenvolvimento da tecnologia envolvendo o campo da regeneração em nervos periféricos, ainda não existe uma técnica para recuperação do tecido nervoso que apresente…
(more)
▼ Apesar do desenvolvimento da tecnologia envolvendo o campo da regeneração em nervos periféricos, ainda não existe uma técnica para recuperação do tecido nervoso que apresente resultados totalmente satisfatórios, fato que desperta o interesse de vários pesquisadores em todo mundo e representa um desafio para os cirurgiões que realizam procedimentos reconstrutivos e estéticos. Embora o enxerto autólogo de nervo seja a melhor alternativa para a recuperação de nervos periféricos lesados, as técnicas que envolvem o reparo tubular têm alcançado excelentes resultados através da utilização de materiais sintéticos e biológicos, sob a forma de tubos, no reparo de extensos segmentos nervosos. Hoje, através dos avanços da engenharia tecidual, novos materiais estão em desenvolvimento, com o objetivo de aliar características microscópicas às técnicas cirúrgicas existentes. O colágeno, que é sabidamente um elemento promotor da proliferação celular e do reparo tecidual, tem sido amplamente utilizado em estudos de regeneração nervosa. Da mesma maneira, o ácido poli-láctico-poli-glicólico (
PLGA), um copolímero sintético, tem apresentado algumas características favoráveis ao processo de regeneração, como biocompatibilidade e propriedades mecânicas adequadas. Com o propósito de facilitar a regeneração nervosa quando ocorre perda tecidual, uma técnica já difundida pode ser destacada: o enxerto de veia invertida, em que a veia jugular externa é utilizada ao avesso, funcionando como um tubo, criando um microambiente para a regeneração nervosa, através da exposição de elementos fundamentais da camada mais externa do vaso (túnica adventícia). Neste trabalho, agregamos como diferencial a utilização de dois tipos de membranas, especialmente desenvolvidas para fins odontológicos, que visam neoformação óssea, em um estudo que visa à regeneração de um nervo periférico misto, o nervo isquiático. As membranas de colágeno e
PLGA foram colocadas na região do enxerto, sob a forma de tubo, com o objetivo de recuperar um segmento de 10 mm do nervo referido. E, por fim, aliamos a utilização de um segmento de 5 mm da veia jugular externa como tecido de preenchimento dos tubos feitos com as membranas, na tentativa de verificar a eficácia das moléculas biológicas presentes neste vaso, porém agora sendo utilizadas diretamente dentro do enxerto. Após um período de 6 semanas, o grupo que apresentou o melhor resultado foi aquele em que foi utilizada a membrana de colágeno, na forma de tubo, preenchida com a veia jugular externa. Nesse grupo, o diâmetro médio das fibras mielínicas apresentou um valor médio de 5,8 µm e espessura média da bainha de mielina de 1,65 µm. Para o período de 12 semanas, entre os grupos analisados, o maior valor médio encontrado para o diâmetro foi de 5,64 µm e 1,31 µm para a espessura, sendo que este resultado foi apresentado pelo grupo em que se utilizou a membrana de
PLGA sem preenchimento com a veia jugular. Embora os valores não sejam muito inferiores, nos dois períodos, ficaram abaixo dos valores encontrados nos grupos…
Advisors/Committee Members: Rodrigues, Antonio de Castro.
Subjects/Keywords: Colágeno; Collagen; Membranas; Membranes; Nervo periférico; Peripheral nerve; PLGA; PLGA; Regeneração; Regeneration; Tubulização; Tubulization
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APA ·
Chicago ·
MLA ·
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CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Melo, C. G. d. S. (2011). Enxerto sintético e biológico, com e sem preenchimento de veia jugular externa, no reparo de nervo periférico em ratos. (Masters Thesis). University of São Paulo. Retrieved from http://www.teses.usp.br/teses/disponiveis/25/25149/tde-24082011-103123/ ;
Chicago Manual of Style (16th Edition):
Melo, Carina Guimarães de Souza. “Enxerto sintético e biológico, com e sem preenchimento de veia jugular externa, no reparo de nervo periférico em ratos.” 2011. Masters Thesis, University of São Paulo. Accessed March 04, 2021.
http://www.teses.usp.br/teses/disponiveis/25/25149/tde-24082011-103123/ ;.
MLA Handbook (7th Edition):
Melo, Carina Guimarães de Souza. “Enxerto sintético e biológico, com e sem preenchimento de veia jugular externa, no reparo de nervo periférico em ratos.” 2011. Web. 04 Mar 2021.
Vancouver:
Melo CGdS. Enxerto sintético e biológico, com e sem preenchimento de veia jugular externa, no reparo de nervo periférico em ratos. [Internet] [Masters thesis]. University of São Paulo; 2011. [cited 2021 Mar 04].
Available from: http://www.teses.usp.br/teses/disponiveis/25/25149/tde-24082011-103123/ ;.
Council of Science Editors:
Melo CGdS. Enxerto sintético e biológico, com e sem preenchimento de veia jugular externa, no reparo de nervo periférico em ratos. [Masters Thesis]. University of São Paulo; 2011. Available from: http://www.teses.usp.br/teses/disponiveis/25/25149/tde-24082011-103123/ ;
19.
Sicchieri, Luciana Gonçalves.
Engenharia de tecido ósseo: avaliações in vitro e in vivo do biomaterial híbrido ácido poli-láctico-co-glicólico/fosfato de cálcio e células osteoblásticas derivadas de células-tronco.
Degree: Mestrado, Cirurgia Buco-Maxilo-Facial, 2010, University of São Paulo
URL: http://www.teses.usp.br/teses/disponiveis/58/58136/tde-17092010-084425/
;
► Tem sido sugerido que um adequado reparo ósseo pode ser obtido por biomateriais híbridos, produzidos pela combinação de células e materiais substitutos ósseos macroporosos. O…
(more)
▼ Tem sido sugerido que um adequado reparo ósseo pode ser obtido por biomateriais híbridos, produzidos pela combinação de células e materiais substitutos ósseos macroporosos. O objetivo geral do presente estudo foi avaliar a aplicação do biomaterial híbrido formado pelo arcabouço de PLGA/CaP e células-tronco mesenquimais e osteoblastos derivados de medula óssea na engenharia de tecido ósseo. Para verificar qual o tamanho de poro deste arcabouço é mais adequado para este fim, foram realizados experimentos in vitro, que avaliaram a proliferação celular, atividade de fosfatase alcalina (ALP) e expressão quantitativa de genes marcadores do fenótipo osteoblástico em células cultivadas sobre os arcabouços; e in vivo, que avaliaram a formação óssea após implantação do arcabouço em defeitos ósseos críticos de calvária de ratos. Também foi avaliado o efeito do tamanho dos poros sobre o carreamento celular através da força centrifuga. Para avaliar o efeito do soro fetal bovino, utilizado na suplementação do meio de cultura celular, na resposta tecidual in vivo, arcabouços expostos ao soro foram implantados em defeitos ósseos críticos de calvária de ratos. O efeito da retirada do soro fetal bovino do meio de cultura sobre osteoblastos foi analisado através da proliferação celular, atividade de ALP, conteúdo de proteína total e mineralização. Para avaliar o efeito do estágio de diferenciação celular sobre a formação óssea, células em diferentes estágios de diferenciação osteoblástica associadas ao arcabouço de PLGA/CaP foram implantadas de forma autóloga em defeitos ósseos críticos de calvária de ratos. Arcabouços com tamanhos de poros de aproximadamente 1000 µm promovem maior diferenciação osteoblástica e melhor carreamento celular, enquanto arcabouços com poros de aproximadamente 500 µm promovem maior formação óssea e vascular in vivo. O soro fetal bovino influenciou negativamente a resposta tecidual e a formação óssea. A retirada do soro fetal bovino do meio de cultura reduziu a proliferação celular e a atividade de ALP sem afetar o conteúdo de proteína total e a formação de matriz mineralizada. Células-tronco mesenquimais indiferenciadas e em fase inicial de diferenciação osteoblástica (7 dias) promoveram maior formação óssea, portanto permitiriam a obtenção de um biomaterial híbrido com maior potencial osteogênico.
It has been suggested that an adequate bone repair can be obtained by hybrid biomaterials, produced by combining osteoprogenitor cells and macroporous bone substitutes. The aim of this study was to evaluate the application of hybrid biomaterial formed by PLGA/CaP scaffold and bone marrow-derived mesenchymal stem cells and osteoblasts in bone tissue engineering. The effect of scaffold pore size was evaluated in vitro assessing cell growth, alkaline phosphatase (ALP) activity, and quantitative gene expression of osteoblastic phenotype markers in osteoblasts cultured on scaffolds; and in vivo assesseing bone formation after implantation of scaffolds in critical size rat calvaria defects. It was also evaluated…
Advisors/Committee Members: Rosa, Adalberto Luiz.
Subjects/Keywords: bone engineering; células osteoprogenitoras; engenharia óssea; osteogênese; osteogenesis; osteoprogenitor cells; PLGA/CaP; PLGA/CaP
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APA (6th Edition):
Sicchieri, L. G. (2010). Engenharia de tecido ósseo: avaliações in vitro e in vivo do biomaterial híbrido ácido poli-láctico-co-glicólico/fosfato de cálcio e células osteoblásticas derivadas de células-tronco. (Masters Thesis). University of São Paulo. Retrieved from http://www.teses.usp.br/teses/disponiveis/58/58136/tde-17092010-084425/ ;
Chicago Manual of Style (16th Edition):
Sicchieri, Luciana Gonçalves. “Engenharia de tecido ósseo: avaliações in vitro e in vivo do biomaterial híbrido ácido poli-láctico-co-glicólico/fosfato de cálcio e células osteoblásticas derivadas de células-tronco.” 2010. Masters Thesis, University of São Paulo. Accessed March 04, 2021.
http://www.teses.usp.br/teses/disponiveis/58/58136/tde-17092010-084425/ ;.
MLA Handbook (7th Edition):
Sicchieri, Luciana Gonçalves. “Engenharia de tecido ósseo: avaliações in vitro e in vivo do biomaterial híbrido ácido poli-láctico-co-glicólico/fosfato de cálcio e células osteoblásticas derivadas de células-tronco.” 2010. Web. 04 Mar 2021.
Vancouver:
Sicchieri LG. Engenharia de tecido ósseo: avaliações in vitro e in vivo do biomaterial híbrido ácido poli-láctico-co-glicólico/fosfato de cálcio e células osteoblásticas derivadas de células-tronco. [Internet] [Masters thesis]. University of São Paulo; 2010. [cited 2021 Mar 04].
Available from: http://www.teses.usp.br/teses/disponiveis/58/58136/tde-17092010-084425/ ;.
Council of Science Editors:
Sicchieri LG. Engenharia de tecido ósseo: avaliações in vitro e in vivo do biomaterial híbrido ácido poli-láctico-co-glicólico/fosfato de cálcio e células osteoblásticas derivadas de células-tronco. [Masters Thesis]. University of São Paulo; 2010. Available from: http://www.teses.usp.br/teses/disponiveis/58/58136/tde-17092010-084425/ ;
Universidade Estadual de Campinas
20.
Calderini, Adriana.
Preparação e caracterização de nano-esferas de PLGA contendo 5-fluorouracil e estudo do acoplamento de quitosana e ácido fólico em sua superfície: Preparation and characterization of PLGA nanospheres containing 5-fluorouracil and study of chitosan and folic acid attachment on their surface.
Degree: 2011, Universidade Estadual de Campinas
URL: http://repositorio.unicamp.br/jspui/handle/REPOSIP/250353
► Abstract: 5-fluorouracil (5-fluoro-1H-pyrimidine-2,4-dione) is one of the most used drug to treat solid tumors in adults, specifically gastrointestinal (stomach and colorectal) and breast carcinomas. In…
(more)
▼ Abstract: 5-fluorouracil (5-fluoro-1H-pyrimidine-2,4-dione) is one of the most used drug to treat solid tumors in adults, specifically gastrointestinal (stomach and colorectal) and breast carcinomas. In summary, the major biochemical effect of 5-FU is inhibition of DNA synthesis, since concentrations which inhibit this synthesis may still permit RNA synthesis. It causes severe adverse effects as myelosuppression, mucositis, dermatitis, diarrhea and cardiac toxicity. Its encapsulation in nanoparticles can reduce these adverse effects, prolong its release and the drug can be placed directly on its site of action. The goal of this work was to encapsulate this anti-neoplasic drug using poly (lactic-co-glycolic acid)
PLGA nanospheres as carrier system, attaching on their surface chitosan and folate-chitosan to attain an enhanced targeting, bioadesivity and less toxicity. The encapsulation of 5-FU in
PLGA nanospheres was improved through an experimental design and chemometry. Many factors were studied: methods of preparation, temperature, initial amount of 5-FU and pH. In the characterization, many techniques were employed: dynamic light scattering, determination of Zeta potential, differential scanning calorimetry, thermo gravimetric analysis, X-ray difractometry and scanning electron microscopy. Besides, we also analyzed the release profile, colloidal stability and the behavior of these systems in relation to in vitro cancer cells. The experimental design allowed obtaining nanoparticles with drug loading around 11% and encapsulation efficiency of 32%. The attachment of chitosan and folate-chitosan also allowed prolonging the drug release in solution. To store these formulations, we observed that lyophilized particles kept at 4 °C were less degraded. The best sucrose concentration to freeze-drying these particles with no size and polidispersity change was 250 mmol/L. The in vitro tests proved the efficacy of these formulations
Advisors/Committee Members: UNIVERSIDADE ESTADUAL DE CAMPINAS (CRUESP), Pessine, Francisco Benedito Teixeira, 1948- (advisor), Universidade Estadual de Campinas. Instituto de Química (institution), Programa de Pós-Graduação em Química (nameofprogram), Silva, Andre Romero da (committee member), Fraceto, Leonardo Fernandes (committee member), Jorge, Renato Atilio (committee member), Volpe, Pedro Luiz Onófrio (committee member).
Subjects/Keywords: 5-Fluorouracil; PLGA; Quitosana; Vitamina M; 5-Fluorouracil; PLGA; Chitosan; Folic acid
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APA (6th Edition):
Calderini, A. (2011). Preparação e caracterização de nano-esferas de PLGA contendo 5-fluorouracil e estudo do acoplamento de quitosana e ácido fólico em sua superfície: Preparation and characterization of PLGA nanospheres containing 5-fluorouracil and study of chitosan and folic acid attachment on their surface. (Thesis). Universidade Estadual de Campinas. Retrieved from http://repositorio.unicamp.br/jspui/handle/REPOSIP/250353
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Calderini, Adriana. “Preparação e caracterização de nano-esferas de PLGA contendo 5-fluorouracil e estudo do acoplamento de quitosana e ácido fólico em sua superfície: Preparation and characterization of PLGA nanospheres containing 5-fluorouracil and study of chitosan and folic acid attachment on their surface.” 2011. Thesis, Universidade Estadual de Campinas. Accessed March 04, 2021.
http://repositorio.unicamp.br/jspui/handle/REPOSIP/250353.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Calderini, Adriana. “Preparação e caracterização de nano-esferas de PLGA contendo 5-fluorouracil e estudo do acoplamento de quitosana e ácido fólico em sua superfície: Preparation and characterization of PLGA nanospheres containing 5-fluorouracil and study of chitosan and folic acid attachment on their surface.” 2011. Web. 04 Mar 2021.
Vancouver:
Calderini A. Preparação e caracterização de nano-esferas de PLGA contendo 5-fluorouracil e estudo do acoplamento de quitosana e ácido fólico em sua superfície: Preparation and characterization of PLGA nanospheres containing 5-fluorouracil and study of chitosan and folic acid attachment on their surface. [Internet] [Thesis]. Universidade Estadual de Campinas; 2011. [cited 2021 Mar 04].
Available from: http://repositorio.unicamp.br/jspui/handle/REPOSIP/250353.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Calderini A. Preparação e caracterização de nano-esferas de PLGA contendo 5-fluorouracil e estudo do acoplamento de quitosana e ácido fólico em sua superfície: Preparation and characterization of PLGA nanospheres containing 5-fluorouracil and study of chitosan and folic acid attachment on their surface. [Thesis]. Universidade Estadual de Campinas; 2011. Available from: http://repositorio.unicamp.br/jspui/handle/REPOSIP/250353
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Universidade Estadual de Campinas
21.
Profirio, Daniel de Moraes, 1989-.
Preparação de nanopartículas de PLGA contendo carboplatina e sua funcionalização com folato de quitosana: Preparation of PLGA nanoparticles containing carboplatin and their functionalization with folic acid-chitosan conjugate.
Degree: 2018, Universidade Estadual de Campinas
URL: http://repositorio.unicamp.br/jspui/handle/REPOSIP/333377
► Abstract: Carboplatin is a cisplatin analogue and non-specific drug that modifies DNA structure and inhibits its replication, causing cell death. Despite the potential antineoplasic effect,…
(more)
▼ Abstract: Carboplatin is a cisplatin analogue and non-specific drug that modifies DNA structure and inhibits its replication, causing cell death. Despite the potential antineoplasic effect, carboplatin causes adverse effects as myelosuppression, thrombocytopenia and its lower cellular uptake by cancer cells contributes to its low therapeutic efficacy. The goal of this work was encapsulation of carboplatin using
PLGA nanoparticles as carrier system, and TPGS as surfactant. Folic acid-conjugated chitosan-coated (FA-CS)
PLGA nanoparticles were also prepared, using experimental design for optimization of both unmodified and surface modified nanoparticles. For
PLGA nanoparticles, the results showed that mean particle size and PDI are dependents of time, amplitude of sonication and volume of aqueous solution, while Zeta potential is constant. The optimized formulation was stable over a period of 60 days when stored at 10ºC in deionized water, and dialysis showed better results as purification method. For surface modified nanoparticles, mean particle size, PDI and Zeta potential were dependent of stirring time and concentration of FA-CS solution. The best conditions for lyophilization of the particles, with no changes in mean particle size and PDI values, were obtained with 96 h and 30% w/V of trehalose. Both formulations showed good stability in PBS pH 7.4. By using fluorescence, the assay with SYBR Green I dye suggested that carboplatin could have maintained its capacity to bind DNA after encapsulation. Diffusion and swelling are involved phenomena in the release process of carboplatin from
PLGA nanoparticles
Advisors/Committee Members: UNIVERSIDADE ESTADUAL DE CAMPINAS (CRUESP), Pessine, Francisco Benedito Teixeira, 1948- (advisor), Universidade Estadual de Campinas. Instituto de Química (institution), Programa de Pós-Graduação em Química (nameofprogram), Ferreira, Marcia Miguel Castro (committee member), Corbi, Pedro Paulo (committee member), Silva, Denise de Oliveira (committee member), Silva, Andre Romero da (committee member).
Subjects/Keywords: Encapsulação; PLGA; Carboplatina; Ácido fólico; Planejamento experimental; Encapsulation; PLGA; Carboplatin; Folic acid; Experimental design
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APA (6th Edition):
Profirio, Daniel de Moraes, 1. (2018). Preparação de nanopartículas de PLGA contendo carboplatina e sua funcionalização com folato de quitosana: Preparation of PLGA nanoparticles containing carboplatin and their functionalization with folic acid-chitosan conjugate. (Thesis). Universidade Estadual de Campinas. Retrieved from http://repositorio.unicamp.br/jspui/handle/REPOSIP/333377
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Profirio, Daniel de Moraes, 1989-. “Preparação de nanopartículas de PLGA contendo carboplatina e sua funcionalização com folato de quitosana: Preparation of PLGA nanoparticles containing carboplatin and their functionalization with folic acid-chitosan conjugate.” 2018. Thesis, Universidade Estadual de Campinas. Accessed March 04, 2021.
http://repositorio.unicamp.br/jspui/handle/REPOSIP/333377.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Profirio, Daniel de Moraes, 1989-. “Preparação de nanopartículas de PLGA contendo carboplatina e sua funcionalização com folato de quitosana: Preparation of PLGA nanoparticles containing carboplatin and their functionalization with folic acid-chitosan conjugate.” 2018. Web. 04 Mar 2021.
Vancouver:
Profirio, Daniel de Moraes 1. Preparação de nanopartículas de PLGA contendo carboplatina e sua funcionalização com folato de quitosana: Preparation of PLGA nanoparticles containing carboplatin and their functionalization with folic acid-chitosan conjugate. [Internet] [Thesis]. Universidade Estadual de Campinas; 2018. [cited 2021 Mar 04].
Available from: http://repositorio.unicamp.br/jspui/handle/REPOSIP/333377.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Profirio, Daniel de Moraes 1. Preparação de nanopartículas de PLGA contendo carboplatina e sua funcionalização com folato de quitosana: Preparation of PLGA nanoparticles containing carboplatin and their functionalization with folic acid-chitosan conjugate. [Thesis]. Universidade Estadual de Campinas; 2018. Available from: http://repositorio.unicamp.br/jspui/handle/REPOSIP/333377
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Université Paris-Sud – Paris XI
22.
Giacalone, Giovanna.
Implant chargé en nanoparticules pour la libération contrôlée et le ciblage lymphatique de nucléotides et d’analogues nucléotidiques : Multi-stage delivery of nucleotides and nucleotide analogs to lymph nodes and leukocytes.
Degree: Docteur es, Pharmacotechnie et biopharmacie, 2014, Université Paris-Sud – Paris XI
URL: http://www.theses.fr/2014PA114845
► Les nucléotides naturels et les analogues nucléotidiques présentent des activités pharmacologiques importantes : par exemple, le nucléotide adénosine triphosphate (ATP) présente un intérêt pour le…
(more)
▼ Les nucléotides naturels et les analogues nucléotidiques présentent des activités pharmacologiques importantes : par exemple, le nucléotide adénosine triphosphate (ATP) présente un intérêt pour le traitement de l'ischémie ou de plaques d'athérosclérose. L'utilisation clinique de ces molécules est cependant limitée en raison de la présence d'un groupe triphosphate, qui est sujet à l'hydrolyse in vivo, et responsable de la forte hydrophilie des molécules, ce qui limite fortement leur capture par les cellules cibles et l'accès à leurs cibles pharmacologiques intracellulaires. Pour surmonter ces limitations et permettre l'administration de nucléotides et d’analogues nucléotidiques, l'utilisation de systèmes de drug delivery comme les nanoparticules pourrait assurer la protection et l'administration ciblée des molécules actives. Cependant, les nanoparticules conçues pour l’administration intraveineuse ne sont pas toujours adaptées au traitement de certaines maladies chroniques. C’est pour cela qu’un implant sous-cutané avec des caractéristiques de libération prolongée peut représenter une alternative valable, tout en étant peu invasif et capable d’atteindre les tissus lymphatiques, cible importante de plusieurs thérapies.Le premier chapitre de cette thèse porte sur la formulation de nanoparticules pour encapsuler l’ATP ou la zidovudine triphosphate (AZT-TP), grâce à la présence du chitosane (CS). Ces nanoparticules sont formées par interactions ioniques entre les charges positives du chitosane et les charges négatives des groupes triphosphates de l’ATP ou de l’AZT-TP. Dans ce travail, les nanoparticules sont caractérisées et leur délivrance cellulaire de l’ATP et de l’AZT-TP est démontrée sur une lignée cellulaire de macrophages. Dans un deuxième temps, la stabilité de ces systèmes a été améliorée afin d'obtenir un meilleur comportement en conditions physiologiques. Cette amélioration de la stabilité a été obtenue par la complexation du fer(III) au chitosane (CS-Fe). Cette stratégie a été appliquée aux nanoparticules de tripolyphosphate (TPP) et d’ATP. Les nanoparticules ont été ensuite testées sur deux lignées de cellules macrophagiques, montrant une internalisation améliorée de l’ATP par rapport aux nanoparticules précédentes. Enfin, les nanoparticules à base de CS-Fe et ATP ont été dispersées dans une solution de PLGA, dans le but de mettre au point un implant à formation in situ. Une fois en contact avec les fluides physiologiques, la suspension prend la forme d’un dépôt solide. Des études de libération in vitro montrent la capacité des systèmes de retenir les nanoparticules à l’intérieur de la matrice et de les libérer de façon progressive pendant 5 jours. Après administration sous-cutanée chez la souris, les implants de PLGA contenant les nanoparticules ont retenu l’ATP au lieu de l’injection jusqu’à 50 heures, comparé à quelques heures pour l’ATP libre et les nanoparticules libres, montrant ainsi leur pertinence comme systèmes pour la libération prolongée de nucléotides.
Natural nucleotides and nucleotide…
Advisors/Committee Members: Fattal, Elias (thesis director), Hillaireau, Hervé (thesis director).
Subjects/Keywords: Nanoparticules; ATP; Chitosane; Libération prolongée; PLGA; Vectorisation; Nanoparticles; ATP; Chitosan; PLGA; Sustained release; Drug delivery
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APA ·
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APA (6th Edition):
Giacalone, G. (2014). Implant chargé en nanoparticules pour la libération contrôlée et le ciblage lymphatique de nucléotides et d’analogues nucléotidiques : Multi-stage delivery of nucleotides and nucleotide analogs to lymph nodes and leukocytes. (Doctoral Dissertation). Université Paris-Sud – Paris XI. Retrieved from http://www.theses.fr/2014PA114845
Chicago Manual of Style (16th Edition):
Giacalone, Giovanna. “Implant chargé en nanoparticules pour la libération contrôlée et le ciblage lymphatique de nucléotides et d’analogues nucléotidiques : Multi-stage delivery of nucleotides and nucleotide analogs to lymph nodes and leukocytes.” 2014. Doctoral Dissertation, Université Paris-Sud – Paris XI. Accessed March 04, 2021.
http://www.theses.fr/2014PA114845.
MLA Handbook (7th Edition):
Giacalone, Giovanna. “Implant chargé en nanoparticules pour la libération contrôlée et le ciblage lymphatique de nucléotides et d’analogues nucléotidiques : Multi-stage delivery of nucleotides and nucleotide analogs to lymph nodes and leukocytes.” 2014. Web. 04 Mar 2021.
Vancouver:
Giacalone G. Implant chargé en nanoparticules pour la libération contrôlée et le ciblage lymphatique de nucléotides et d’analogues nucléotidiques : Multi-stage delivery of nucleotides and nucleotide analogs to lymph nodes and leukocytes. [Internet] [Doctoral dissertation]. Université Paris-Sud – Paris XI; 2014. [cited 2021 Mar 04].
Available from: http://www.theses.fr/2014PA114845.
Council of Science Editors:
Giacalone G. Implant chargé en nanoparticules pour la libération contrôlée et le ciblage lymphatique de nucléotides et d’analogues nucléotidiques : Multi-stage delivery of nucleotides and nucleotide analogs to lymph nodes and leukocytes. [Doctoral Dissertation]. Université Paris-Sud – Paris XI; 2014. Available from: http://www.theses.fr/2014PA114845
23.
Albert, Claire.
Émulsions de Pickering biodégradables stabilisées par des nanoparticules de poly(acide lactique-co-glycolique) : étude physico-chimique et potentialité pharmaceutique : Biodegradable Pickering emulsions stabilized with poly(lactic-co-glycolic acid) nanoparticles : physico-chemical study and pharmaceutical potentiality.
Degree: Docteur es, Pharmacotechnie et biopharmacie, 2017, Université Paris-Saclay (ComUE)
URL: http://www.theses.fr/2017SACLS491
► Dans ce travail de thèse, nous avons formulé des émulsions de Pickering stables, biodégradables et biocompatibles stabilisées par des nanoparticules (NPs) de poly(acide lactique-co-glycolique) (PLGA).…
(more)
▼ Dans ce travail de thèse, nous avons formulé des émulsions de Pickering stables, biodégradables et biocompatibles stabilisées par des nanoparticules (NPs) de poly(acide lactique-co-glycolique) (PLGA). De telles émulsions sont une alternative, potentiellement moins toxique et irritante, aux émulsions conventionnelles stabilisées par des tensioactifs synthétiques. Dans un premier temps, une étude physico-chimique approfondie de ces systèmes a permis de clarifier leurs structures (macroscopique, microscopique et interfaciale) ainsi que leurs mécanismes et leurs cinétiques de stabilisation. Des études de la contribution du polymère stabilisant les NPs et des caractéristiques du polymère de PLGA utilisé sur les propriétés des émulsions ont également été réalisées. Cela a permis de mieux identifier les paramètres physico-chimiques clés nécessaires à une bonne stabilisation. Dans un second temps, nous nous sommes intéressés au potentiel pharmaceutique de ces émulsions pour une application topique. Des substances actives (SA), utilisées pour le traitement du psoriasis, ont été encapsulées avec succès dans les NPs (ciclosporine A et tacrolimus) et les gouttelettes de l’émulsion (calcitriol). Cette étude est un premier pas vers l’utilisation de ces émulsions pour la co-encapsulation de deux SA dans la même formulation : une première dans les NPs et une seconde dans les gouttelettes d’huile. La co-encapsulation devrait permettre d’améliorer l’observance du patient et pourrait conduire à un effet synergique entre les deux SA.
In this thesis work, we formulated stable, biodegradable and biocompatible Pickering emulsions stabilized with nanoparticles (NPs) of poly(lactic-co-glycolic acid) (PLGA). Such emulsions are an alternative, potentially less toxic and irritating, to conventional emulsions stabilized with surfactants. Firstly, a thorough physico-chemical study of these systems was conducted in order to clarify their structures (macroscopic, microscopic and interfacial) as well as their mechanisms and kinetics of stabilization. Studies of the contribution of the polymer stabilizing the NPs and of the characteristics of the PLGA polymer on the properties of the emulsions were also carried out. This enabled a better identification of the physico-chemical key parameters responsible for a good stabilization. Secondly, we focused on the pharmaceutical potential of these emulsions for a topical application. Pharmaceutical active ingredients (API), used for the treatment of psoriasis, were successfully encapsulated in the NPs (cyclosporine A and tacrolimus) and the emulsion droplets (calcitriol). This study is a first step towards the use of these emulsions for the co-encapsulation of two API: one in the NPs and a second in the oil droplets. The co-encapsulation should improve patient compliance and could lead to a synergistic effect between the two API.
Advisors/Committee Members: Agnely, Florence (thesis director), Huang, Nicolas (thesis director).
Subjects/Keywords: Émulsion; Nanoparticule; Plga; Interface; Microstructure; Encapsulation; Emulsion; Nanoparticle; Plga; Interface; Microstructure; Encapsulation
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APA ·
Chicago ·
MLA ·
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CSE |
Export
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APA (6th Edition):
Albert, C. (2017). Émulsions de Pickering biodégradables stabilisées par des nanoparticules de poly(acide lactique-co-glycolique) : étude physico-chimique et potentialité pharmaceutique : Biodegradable Pickering emulsions stabilized with poly(lactic-co-glycolic acid) nanoparticles : physico-chemical study and pharmaceutical potentiality. (Doctoral Dissertation). Université Paris-Saclay (ComUE). Retrieved from http://www.theses.fr/2017SACLS491
Chicago Manual of Style (16th Edition):
Albert, Claire. “Émulsions de Pickering biodégradables stabilisées par des nanoparticules de poly(acide lactique-co-glycolique) : étude physico-chimique et potentialité pharmaceutique : Biodegradable Pickering emulsions stabilized with poly(lactic-co-glycolic acid) nanoparticles : physico-chemical study and pharmaceutical potentiality.” 2017. Doctoral Dissertation, Université Paris-Saclay (ComUE). Accessed March 04, 2021.
http://www.theses.fr/2017SACLS491.
MLA Handbook (7th Edition):
Albert, Claire. “Émulsions de Pickering biodégradables stabilisées par des nanoparticules de poly(acide lactique-co-glycolique) : étude physico-chimique et potentialité pharmaceutique : Biodegradable Pickering emulsions stabilized with poly(lactic-co-glycolic acid) nanoparticles : physico-chemical study and pharmaceutical potentiality.” 2017. Web. 04 Mar 2021.
Vancouver:
Albert C. Émulsions de Pickering biodégradables stabilisées par des nanoparticules de poly(acide lactique-co-glycolique) : étude physico-chimique et potentialité pharmaceutique : Biodegradable Pickering emulsions stabilized with poly(lactic-co-glycolic acid) nanoparticles : physico-chemical study and pharmaceutical potentiality. [Internet] [Doctoral dissertation]. Université Paris-Saclay (ComUE); 2017. [cited 2021 Mar 04].
Available from: http://www.theses.fr/2017SACLS491.
Council of Science Editors:
Albert C. Émulsions de Pickering biodégradables stabilisées par des nanoparticules de poly(acide lactique-co-glycolique) : étude physico-chimique et potentialité pharmaceutique : Biodegradable Pickering emulsions stabilized with poly(lactic-co-glycolic acid) nanoparticles : physico-chemical study and pharmaceutical potentiality. [Doctoral Dissertation]. Université Paris-Saclay (ComUE); 2017. Available from: http://www.theses.fr/2017SACLS491
24.
Beladjine, Mohamed.
Co-encapsulation d'un agent immunosuppresseur et d'un agent anti-inflammatoire dans une émulsion de Pickering pour le traitement topique de pathologies cutanées : Co-encapsulation of immunosuppressant and anti-inflammatory agents in a Pickering emulsion for the topical treatment of skin diseases.
Degree: Docteur es, Pharmacotechnie et biopharmacie, 2019, Université Paris-Saclay (ComUE)
URL: http://www.theses.fr/2019SACLS514
► Dans ce travail de thèse, nous avons préparées des émulsions de Pickering innovantes, stabilisées par des nanoparticules (NP) biocompatibles et biodégradables de poly (acide lactique-co-glycolique)…
(more)
▼ Dans ce travail de thèse, nous avons préparées des émulsions de Pickering innovantes, stabilisées par des nanoparticules (NP) biocompatibles et biodégradables de poly (acide lactique-co-glycolique) (PLGA), en utilisant du Miglyol comme phase huileuse. Ces systèmes permettent de s’affranchir de l’ajout de tensioactifs synthétiques, utilisés pour stabiliser les émulsions classiques, et qui sont souvent à l’origine d’irritations lors de traitements topiques chroniques. Dans ces émulsions ont été co-encapsulées deux substances actives, la première, un agent immunosuppresseur, dans les NP PLGA, la seconde, un agent anti-inflammatoire, dans les gouttelettes de Miglyol.Dans un premier temps, une étude physicochimique approndie a permis de mieux comprendre les mécanismes de stabilisation de ces émulsions, en fonction du type de NP utilisé. Aussi, l’influence de l’ajout de substances actives a été évaluée sur les structures macroscopique, microscopique et interfaciale des émulsions, mais également sur leur stabilité. Dans un deuxième temps, nous avons démontré que l’ajout de Carbopol, un polymère épaississant, permettait d’améliorer la stabilité, la texture et le pH des émulsions. Enfin, le potentiel thérapeutique d’un tel système à été évalué de manière préliminaire, notamment sa cytotoxicité sur des cultures de kératinocytes, mais aussi son activité immunosuppressive sur des cellules immunitaires activées et leur production de cytokines. Un tel système peut donc s’avérer intéressant pour le traitement de pathologies cutanées chroniques.
In this thesis work, we formulated innovative Pickering emulsions, stabilized by biocompatible and biodegradable poly (lactic-co-glycolic acid) nanoparticles (NP), using Miglyol as the oil phase. These systems could be an alternative to conventional emulsions, often stabilized with synthetic surfactants that can be responsible for irritation in particular during long-term topical treatments. Two active pharmaceutical ingredients (API) were successfully co-encapsulated, the first one, an immunosuppressant agent, entrapped in PLGA NP, the second one, an anti-inflammatory agent, incorporated in Miglyol droplets.Firstly, a thorough physicochemical characterization allowed to better understand the stabilization mechanism of these emulsions, depending on the type of NP used. Moreover, the influence of the addition of API was evaluated on the macroscopic, microscopic and interfacial structures of the emulsions, also on their stability. Secondly, we demonstrated that the addition of Carbopol, a thickening agent, improved the stability, texture and pH of emulsions. Finally, the therapeutic potential of such system was investigated through preliminary evaluation of its cytotoxicity on keratinocyte cells, but also its immunosuppressive activity on activated immune cells and their cytokine production. Such a system could be interesting for the treatment of chronical skin diseases.
Advisors/Committee Members: Agnely, Florence (thesis director), Huang, Nicolas (thesis director).
Subjects/Keywords: Nanoparticules; Plga; Formulation; Administration topique; Emulsions; Psoriasis; Plga; Nanoparticles; Formulation; Psoriasis; Topical administration; Emulsions
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APA (6th Edition):
Beladjine, M. (2019). Co-encapsulation d'un agent immunosuppresseur et d'un agent anti-inflammatoire dans une émulsion de Pickering pour le traitement topique de pathologies cutanées : Co-encapsulation of immunosuppressant and anti-inflammatory agents in a Pickering emulsion for the topical treatment of skin diseases. (Doctoral Dissertation). Université Paris-Saclay (ComUE). Retrieved from http://www.theses.fr/2019SACLS514
Chicago Manual of Style (16th Edition):
Beladjine, Mohamed. “Co-encapsulation d'un agent immunosuppresseur et d'un agent anti-inflammatoire dans une émulsion de Pickering pour le traitement topique de pathologies cutanées : Co-encapsulation of immunosuppressant and anti-inflammatory agents in a Pickering emulsion for the topical treatment of skin diseases.” 2019. Doctoral Dissertation, Université Paris-Saclay (ComUE). Accessed March 04, 2021.
http://www.theses.fr/2019SACLS514.
MLA Handbook (7th Edition):
Beladjine, Mohamed. “Co-encapsulation d'un agent immunosuppresseur et d'un agent anti-inflammatoire dans une émulsion de Pickering pour le traitement topique de pathologies cutanées : Co-encapsulation of immunosuppressant and anti-inflammatory agents in a Pickering emulsion for the topical treatment of skin diseases.” 2019. Web. 04 Mar 2021.
Vancouver:
Beladjine M. Co-encapsulation d'un agent immunosuppresseur et d'un agent anti-inflammatoire dans une émulsion de Pickering pour le traitement topique de pathologies cutanées : Co-encapsulation of immunosuppressant and anti-inflammatory agents in a Pickering emulsion for the topical treatment of skin diseases. [Internet] [Doctoral dissertation]. Université Paris-Saclay (ComUE); 2019. [cited 2021 Mar 04].
Available from: http://www.theses.fr/2019SACLS514.
Council of Science Editors:
Beladjine M. Co-encapsulation d'un agent immunosuppresseur et d'un agent anti-inflammatoire dans une émulsion de Pickering pour le traitement topique de pathologies cutanées : Co-encapsulation of immunosuppressant and anti-inflammatory agents in a Pickering emulsion for the topical treatment of skin diseases. [Doctoral Dissertation]. Université Paris-Saclay (ComUE); 2019. Available from: http://www.theses.fr/2019SACLS514
25.
Rigaux, Guillaume.
Elaboration, caractérisation et évaluation biologique de nanoparticules biocompatibles pour la thérapie photodynamique et l’imagerie IRM : Elaboration, characterization and biological evaluation of biocompatible nanoparticles for photodynamic therapy and MRI.
Degree: Docteur es, Sciences - STS, 2015, Reims
URL: http://www.theses.fr/2015REIMS026
► L'objectif poursuivi au cours de ce travail est l'élaboration de nanoparticules biocompatibles à visée diagnostique (IRM) et thérapeutique (PDT). Dans ce but, un protocole de…
(more)
▼ L'objectif poursuivi au cours de ce travail est l'élaboration de nanoparticules biocompatibles à visée diagnostique (IRM) et thérapeutique (PDT). Dans ce but, un protocole de nanoprécipitation a été optimisé pour obtenir de façon quantitative et reproductible, des nanoparticules de PLGA de diamètre compatible avec une injection par voie parentérale. Cette formulation a été employée avec succès pour l'encapsulation d'un chélate lipophile de Gd(III), pour l'encapsulation d'un photosensibilisateur (m-THPC) et pour la co-encapsulation de ces deux substances actives. Les formulations optimales permettent d'obtenir des efficacités d'encapsulation de 7 et 46 % en chélate de gadolinium et m-THPC respectivement. La cytotoxicité et la photocytotoxicité des [email protected]PLGA ont été testées sur deux lignées cellulaires (C6 et fibroblastes) et les résultats obtenus montrent que les propriétés photocytotoxiques du m-THPC sont maintenues après l'encapsulation. L'efficacité IRM de ces nanoparticules a aussi été évaluée et les mesures NMRD et IRM à 3T montrent que l'encapsulation des chélates de gadolinium améliore leur capacité à générer un contraste en mode T1 et donc la qualité des images.
This work aimed at the synthesis of biocompatible nanoparticles for PDT and MRI applications. To reach this goal, a nanoprecipitation technique was optimized using only biocompatible starting materials. This technique allowed the preparation, in a reproducible and quantitative manner of PLGA nanoparticles, compatible with parenteral injections. This formulation was successfully applied to encapsulate a lipophilic Gd(III) chelate, a photosensitizer (m-THPC) and to co-encapsulate these two substances. Optimal formulations showed encapsulation yields of 7 and 46 % for the gadolinium chelate and m-THPC, respectively. Cytotoxicity and photocytotoxicity experiments performed for two different cell lines (C6 cells and fibroblasts) incubated with [email protected]PLGA nanoparticles showed that m-THPC photocytotoxicity was maintained after its encapsulation. MRI efficacy of [email protected]PLGA nanoparticles was also evaluated by NMRD measurements and 3T MRI images. The corresponding results indicated that gadolinium chelate encapsulation improved its tendency to generate an efficient T1 contrast and consequently, enhanced the image contrast.
Advisors/Committee Members: Chuburu, Françoise (thesis director).
Subjects/Keywords: Nanoparticules de PLGA; Nanoprécipitation; Photosensibilisateurs; Pdt; Gadolinium; Irm; PLGA nanoparticles; Nanoprecipitation; Photosensitizer; Pdt; Gadolinium; Mri
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APA (6th Edition):
Rigaux, G. (2015). Elaboration, caractérisation et évaluation biologique de nanoparticules biocompatibles pour la thérapie photodynamique et l’imagerie IRM : Elaboration, characterization and biological evaluation of biocompatible nanoparticles for photodynamic therapy and MRI. (Doctoral Dissertation). Reims. Retrieved from http://www.theses.fr/2015REIMS026
Chicago Manual of Style (16th Edition):
Rigaux, Guillaume. “Elaboration, caractérisation et évaluation biologique de nanoparticules biocompatibles pour la thérapie photodynamique et l’imagerie IRM : Elaboration, characterization and biological evaluation of biocompatible nanoparticles for photodynamic therapy and MRI.” 2015. Doctoral Dissertation, Reims. Accessed March 04, 2021.
http://www.theses.fr/2015REIMS026.
MLA Handbook (7th Edition):
Rigaux, Guillaume. “Elaboration, caractérisation et évaluation biologique de nanoparticules biocompatibles pour la thérapie photodynamique et l’imagerie IRM : Elaboration, characterization and biological evaluation of biocompatible nanoparticles for photodynamic therapy and MRI.” 2015. Web. 04 Mar 2021.
Vancouver:
Rigaux G. Elaboration, caractérisation et évaluation biologique de nanoparticules biocompatibles pour la thérapie photodynamique et l’imagerie IRM : Elaboration, characterization and biological evaluation of biocompatible nanoparticles for photodynamic therapy and MRI. [Internet] [Doctoral dissertation]. Reims; 2015. [cited 2021 Mar 04].
Available from: http://www.theses.fr/2015REIMS026.
Council of Science Editors:
Rigaux G. Elaboration, caractérisation et évaluation biologique de nanoparticules biocompatibles pour la thérapie photodynamique et l’imagerie IRM : Elaboration, characterization and biological evaluation of biocompatible nanoparticles for photodynamic therapy and MRI. [Doctoral Dissertation]. Reims; 2015. Available from: http://www.theses.fr/2015REIMS026
Université de Lorraine
26.
Riffault, Mathieu.
Évaluation biologique d’un vecteur nanoparticulaire à visée ostéoarticulaire : Biological evaluation of a nanostructured vector for osteoarticular pathologies.
Degree: Docteur es, Sciences de la vie et de la santé, 2015, Université de Lorraine
URL: http://www.theses.fr/2015LORR0094
► Un des écueils du traitement des pathologies ostéoarticulaires est l’adressage de molécules aux cellules et tissus qui constituent l’articulation. L’administration de principes actifs par voie…
(more)
▼ Un des écueils du traitement des pathologies ostéoarticulaires est l’adressage de molécules aux cellules et tissus qui constituent l’articulation. L’administration de principes actifs par voie sanguine ou orale nécessite l’apport de quantités importantes et peut causer des effets indésirables. Dans ces travaux, nous avons évalué un système d’adressage de molécules hydrophiles. Des particules de polymère d’acide lactique et glycolique, marquées par un traceur fluorescent ont été évaluées in vitro puis in vivo. Ces particules possèdent un recouvrement en acide hyaluronique pour améliorer les interactions avec les cellules. Les études réalisées ont permis de mettre en évidence l’internalisation des particules dans les synoviocytes, chondrocytes, et cellules souches mésenchymateuses après 8 heures d’exposition. Parallèlement, l’évaluation in vitro de différents paramètres de cytotoxicité et d’inflammation a permis de souligner la compatibilité de ces vecteurs avec les cellules articulaires. Enfin, les effets de l’injection intra-articulaire de particules ont été évalués chez le rat sain et différents modèles pathologiques. L’analyse histologique des articulations a démontré l’absence de dégradation de la matrice cartilagineuse et de réaction inflammatoire suite à l’injection de particules. Il a été également constaté un ciblage majoritaire des particules vers la membrane synoviale. Ces travaux permettent de valider l’utilisation de ce type de vecteur pour le ciblage de l’articulation et l’apport de principes actifs. L’association de ces particules à des molécules hydrophiles (acides nucléiques, médicaments,…), pourra permettre leur apport direct dans l’articulation et ouvrir de nouvelles perspectives thérapeutiques.
One of the major issues with treatment of osteoarticular diseases is reaching cells or tissue inside the joint. Administration of an active compound by intravenous or per os requires elevated amount to be effective, and can initiate systemic and negative side effects. In this work we have evaluated a drug delivery system for hydrophilic molecule, which can be used for articular tissues. Polymeric nanoparticles of poly lactic-co-glycolic acid, labelled with a fluorescent dye were evaluated both in vitro and in vivo. These nanoparticles are covered with hyaluronic acid to favor particles-cells interaction. We demonstrated here that such particles are internalized after 8 hours of exposure in synoviocytes, chondrocytes and mesenchymal stem cells. Evaluation of cytotoxicity and inflammation revealed the neutrality of these particles for articular cell types. Finally, intraarticular injections of particles were performed in healthy rat joint and in pathological models. Histological analyses of extracellular matrix integrity and inflammatory status do not demonstrate any side-effect following particles injections. These healthy nano-device target primarily synovial cells, and their degradation inside cells does not provoke deleterious effects. This PLGA based drug delivery system would be used to safely deliver…
Advisors/Committee Members: Gillet, Pierre (thesis director), Grossin, Laurent (thesis director).
Subjects/Keywords: Nanoparticules; PLGA; Articulation; Acide hyaluronique; Vectorisation; Nanoparticles; PLGA; Joint; Hyaluronic acid; Vectorization; 615.7
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APA ·
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APA (6th Edition):
Riffault, M. (2015). Évaluation biologique d’un vecteur nanoparticulaire à visée ostéoarticulaire : Biological evaluation of a nanostructured vector for osteoarticular pathologies. (Doctoral Dissertation). Université de Lorraine. Retrieved from http://www.theses.fr/2015LORR0094
Chicago Manual of Style (16th Edition):
Riffault, Mathieu. “Évaluation biologique d’un vecteur nanoparticulaire à visée ostéoarticulaire : Biological evaluation of a nanostructured vector for osteoarticular pathologies.” 2015. Doctoral Dissertation, Université de Lorraine. Accessed March 04, 2021.
http://www.theses.fr/2015LORR0094.
MLA Handbook (7th Edition):
Riffault, Mathieu. “Évaluation biologique d’un vecteur nanoparticulaire à visée ostéoarticulaire : Biological evaluation of a nanostructured vector for osteoarticular pathologies.” 2015. Web. 04 Mar 2021.
Vancouver:
Riffault M. Évaluation biologique d’un vecteur nanoparticulaire à visée ostéoarticulaire : Biological evaluation of a nanostructured vector for osteoarticular pathologies. [Internet] [Doctoral dissertation]. Université de Lorraine; 2015. [cited 2021 Mar 04].
Available from: http://www.theses.fr/2015LORR0094.
Council of Science Editors:
Riffault M. Évaluation biologique d’un vecteur nanoparticulaire à visée ostéoarticulaire : Biological evaluation of a nanostructured vector for osteoarticular pathologies. [Doctoral Dissertation]. Université de Lorraine; 2015. Available from: http://www.theses.fr/2015LORR0094
Aristotle University Of Thessaloniki (AUTH); Αριστοτέλειο Πανεπιστήμιο Θεσσαλονίκης (ΑΠΘ)
27.
Katsikari, Athanasia.
Ανάπτυξη μεθόδων μεταφοράς βιομορίων με τη χρήση νανοσωματιδίων.
Degree: 2014, Aristotle University Of Thessaloniki (AUTH); Αριστοτέλειο Πανεπιστήμιο Θεσσαλονίκης (ΑΠΘ)
URL: http://hdl.handle.net/10442/hedi/41653
► Several problems associated with free drugs and vaccines such as lowsolubility, poor stability and unwanted side effects, had led to developingnovel drug delivery systems. Although…
(more)
▼ Several problems associated with free drugs and vaccines such as lowsolubility, poor stability and unwanted side effects, had led to developingnovel drug delivery systems. Although various innovative delivery systemshave been introduced, there still remains need for further improvement of theissues. In the past few decades particle-based delivery systems have beenenormously investigated to resolve these problems.In particular, the use of biodegradable polymeric nanoparticles withentrapped drugs or antigens represents an exciting approach for controllingthe release of drugs and vaccines. Among polymeric particles, poly (D, Llactide-co-glycolide) (PLGA)–based nanoparticles (NPs) are one of the mostfrequently studied delivery carriers for drugs, peptides, proteins, vaccines andnucleotides. PLGA is a copolymer composed of lactic acid and glycolic acidand is one of a few polymers approved for medical purposes due to itsbiodegradability and nontoxicity.The aim of the present thesis was the production of biodegradablePLGA NPs for efficient protein and DNA delivery to cells and tissues.Nanoparticles were produced by the double emulsion and solvent evaporationtechnique, after the determination of several process parameters affectingtheir physicochemical properties. The produced nanoparticles were fullycharacterized with respect to size, surface charge and morphology. Therelease profile of the encapsulated DNA, as well as the integrity of DNAextracted from nanoparticles, were also examined.The evaluation of potential NP toxicity showed that cell viability isreduced only at nanoparticles concentrations greater than 1 mg/ml. Uptakestudies revealed that nanoparticles were internalized as early as within 30minutes after incubation and that they were distributed around the cytoplasmand the peri-nuclear space. Moreover, the in vitro uptake studiesdemonstrated that cellular uptake of nanoparticles is a concentration-, timeandtemperature- dependent process and that the uptake was inhibitedsignificantly by lowering the incubation temperature at 4ºC, and by thepresence of inhibitors like sodium azide and cytochalasin D. The aboveresults suggest that uptake is an active process and that endocytosis is themain internalization mechanism of nanoparticles in vitro. After first-line in vitro assays, characterization of cellular effects by thedorsal air pouch model was straightforward. It was clearly established thatPLGA NPs possess in vivo immunomodulatory activity since they attractpolymorphonuclear cells (PMNs). Furthermore, intramuscular injection ofpMAX-GFP NPs resulted in enhanced GFP expression in quadriceps musclein Balb/c mice. Finally, it was demonstrated that after oral administration ofFITC loaded PLGA NPs in Balb/c mice nanoparticles can be detected in theintestine within 30 minutes after feeding. Significant levels of gene expressionit was also detected when mice were fed with PLGA NPs containing pMAXGFPplasmid, 12 days after the first immunization.Consequently, the results of the present doctoral thesis underline thepotential of…
Subjects/Keywords: Νανοσωματίδια PLGA; Κυτταροτοξικότητα; Πρόσληψη νανοσωματιδίων; Μεταφορά DNA; PLGA nanoparticles; Cytotoxicity; Nanoparticle uptake; DNA delivery
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APA (6th Edition):
Katsikari, A. (2014). Ανάπτυξη μεθόδων μεταφοράς βιομορίων με τη χρήση νανοσωματιδίων. (Thesis). Aristotle University Of Thessaloniki (AUTH); Αριστοτέλειο Πανεπιστήμιο Θεσσαλονίκης (ΑΠΘ). Retrieved from http://hdl.handle.net/10442/hedi/41653
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Katsikari, Athanasia. “Ανάπτυξη μεθόδων μεταφοράς βιομορίων με τη χρήση νανοσωματιδίων.” 2014. Thesis, Aristotle University Of Thessaloniki (AUTH); Αριστοτέλειο Πανεπιστήμιο Θεσσαλονίκης (ΑΠΘ). Accessed March 04, 2021.
http://hdl.handle.net/10442/hedi/41653.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Katsikari, Athanasia. “Ανάπτυξη μεθόδων μεταφοράς βιομορίων με τη χρήση νανοσωματιδίων.” 2014. Web. 04 Mar 2021.
Vancouver:
Katsikari A. Ανάπτυξη μεθόδων μεταφοράς βιομορίων με τη χρήση νανοσωματιδίων. [Internet] [Thesis]. Aristotle University Of Thessaloniki (AUTH); Αριστοτέλειο Πανεπιστήμιο Θεσσαλονίκης (ΑΠΘ); 2014. [cited 2021 Mar 04].
Available from: http://hdl.handle.net/10442/hedi/41653.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Katsikari A. Ανάπτυξη μεθόδων μεταφοράς βιομορίων με τη χρήση νανοσωματιδίων. [Thesis]. Aristotle University Of Thessaloniki (AUTH); Αριστοτέλειο Πανεπιστήμιο Θεσσαλονίκης (ΑΠΘ); 2014. Available from: http://hdl.handle.net/10442/hedi/41653
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
28.
Pereira, Priscilla Aparecida Tartari.
Efeitos antagônicos da prostaglandina D2 e prostaglandina E2 na resposta imune durante infecção experimental por Histoplasma capsulatum.
Degree: PhD, Imunologia Básica e Aplicada, 2013, University of São Paulo
URL: http://www.teses.usp.br/teses/disponiveis/17/17147/tde-06012014-081806/
;
► O Histoplasma capsulatum é um fungo dimórfico, patogênico e responsável por graves lesões pulmonares. A infecção é adquirida pela inalação de conídios e posterior conversão…
(more)
▼ O Histoplasma capsulatum é um fungo dimórfico, patogênico e responsável por graves lesões pulmonares. A infecção é adquirida pela inalação de conídios e posterior conversão para leveduras nos alvéolos e bronquíolos, onde são fagocitadas por macrófagos alveolares residentes e leucócitos que migram para o local da infecção. Recentemente, demonstramos que animais infectados com H. capsulatum e tratados com inibidor da síntese de prostaglandinas apresentaram diminuição de carga fúngica nos pulmões e baço, aumento da produção de nitrito e da fagocitose de leveduras por macrófagos alveolares, e maior sobrevivência, quando comparados com os animais somente infectados. Porém, neste estudo não foram determinados quais subtipos de prostaglandinas participam na patogênese da histoplasmose. Vários grupos de pesquisa têm demonstrado que PGD2 e PGE2 podem ter ações biológicas distintas quanto à remoção de microrganismos no hospedeiro. Desta maneira, é fundamental o entendimento do papel da PGD2 e da PGE2 nos mecanismos efetores dos macrófagos na defesa do hospedeiro, especialmente na histoplasmose. Portanto, o objetivo deste estudo foi investigar a participação da PGD2 e PGE2 na infecção experimental por H. capsulatum. Assim, demonstramos que a PGD2 aumentou a fagocitose e mecanismos microbicidas de macrófagos alveolares infectados in vitro com H. capsulatum. Observamos ainda que a 15dPGJ2, metabólito da PGD2, aumentou somente a fagocitose, e PGE2 inibiu os mecanismos efetores do macrófago. Mostramos ainda o aumento de BLT1 em macrófagos alveolares após adição de PGD2, e a possível ligação desta ao BLT1, e de LTB4 em DP2. Além disso, caracterizamos micropartículas de
PLGA contendo PGD2 (MS-PGD2), e investigamos seus efeitos. O tamanho, carga elétrica e morfologia das micropartículas foram adequados para um tratamento intranasal e para fagocitose por macrófagos alveolares. As MS-PGD2 foram fagocitadas e capazes de ativar NF-B, e consequentemente, influenciar na produção de nitrito, IL-1, TNF-, IL-6 e TGF-. Com base nestes dados, avaliamos os efeitos do tratamento da MS-PGD2 ou da MS-PGE2 em animais infectados com H. capsulatum. Estas foram administradas via intranasal em animais infectados e tratados ou não com celecoxibe. Verificamos a diminuição da carga fúngica nos pulmões e baço, diminuição do infiltrador celular no espaço broncoalveolar e de citocinas inflamatórias no pulmão após tratamento com MS-PGD2. Contrariamente, após tratamento da MS-PGE2 observamos maior carga fúngica nos pulmões e baço, e aumento da inflamação no tecido e maior produção de IL-10. Além disso, demonstramos que no 21° dia após infecção, referente ao 7° dia após o término do tratamento com MS-PGD2, a carga fúngica manteve-se reduzida nos pulmões, comprovando assim a eficácia deste tratamento. Posteriormente, utilizando inibidores específicos, HQL-79 e CAY10526, mostramos respectivamente o papel protetor da PGD2 e o deletério da PGE2 na histoplasmose. Em conjunto, nossos dados contribuíram para o entendimento das funções antagônicas da PGD2 e PGE2 nesta…
Advisors/Committee Members: Faccioli, Lucia Helena.
Subjects/Keywords: Alveolar macrophage; Histoplasma capsulatum; Histoplasma capsulatum; Macrófago alveolar; PLGA; PLGA; Prostaglandin D2; Prostaglandin E2; Prostaglandina D2; Prostaglandina E2
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APA ·
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APA (6th Edition):
Pereira, P. A. T. (2013). Efeitos antagônicos da prostaglandina D2 e prostaglandina E2 na resposta imune durante infecção experimental por Histoplasma capsulatum. (Doctoral Dissertation). University of São Paulo. Retrieved from http://www.teses.usp.br/teses/disponiveis/17/17147/tde-06012014-081806/ ;
Chicago Manual of Style (16th Edition):
Pereira, Priscilla Aparecida Tartari. “Efeitos antagônicos da prostaglandina D2 e prostaglandina E2 na resposta imune durante infecção experimental por Histoplasma capsulatum.” 2013. Doctoral Dissertation, University of São Paulo. Accessed March 04, 2021.
http://www.teses.usp.br/teses/disponiveis/17/17147/tde-06012014-081806/ ;.
MLA Handbook (7th Edition):
Pereira, Priscilla Aparecida Tartari. “Efeitos antagônicos da prostaglandina D2 e prostaglandina E2 na resposta imune durante infecção experimental por Histoplasma capsulatum.” 2013. Web. 04 Mar 2021.
Vancouver:
Pereira PAT. Efeitos antagônicos da prostaglandina D2 e prostaglandina E2 na resposta imune durante infecção experimental por Histoplasma capsulatum. [Internet] [Doctoral dissertation]. University of São Paulo; 2013. [cited 2021 Mar 04].
Available from: http://www.teses.usp.br/teses/disponiveis/17/17147/tde-06012014-081806/ ;.
Council of Science Editors:
Pereira PAT. Efeitos antagônicos da prostaglandina D2 e prostaglandina E2 na resposta imune durante infecção experimental por Histoplasma capsulatum. [Doctoral Dissertation]. University of São Paulo; 2013. Available from: http://www.teses.usp.br/teses/disponiveis/17/17147/tde-06012014-081806/ ;
29.
Violet, Fabien.
Développement d’une protéine à libération prolongée, mise au point du procédé d’encapsulation sans solvant halogéné et optimisation du profil de libération. : Development of microencapsulation process without toxic solvent, application to sustained protein release.
Degree: Docteur es, Pharmacie, 2015, Angers
URL: http://www.theses.fr/2015ANGE0040
► La régénération tissulaire est une voie prometteuse de thérapie dans le cadre des maladies dégénératives. Dans ce but sont conçus les microcarriers pharmacologiquement actifs (PAM).…
(more)
▼ La régénération tissulaire est une voie prometteuse de thérapie dans le cadre des maladies dégénératives. Dans ce but sont conçus les microcarriers pharmacologiquement actifs (PAM). Ce sont des microsphères fournissant un environnement adéquat à la survie et la différenciation de cellules souches par la libération d’un facteur de croissance protéique encapsulé.Pour potentialiser l’intérêt des PAM, les microsphères doivent (1) permettre la libération complète et prolongée de la protéine (2) être formulées sans solvant halogéné par un procédé transposable à l’échelle pilote.Deux stratégies sont menées afin d’améliorer la stabilité et la libération de la protéine. La première consiste à utiliser de nouveaux additifs. Une étude bibliographique révèle le potentiel d’additifs protéiques ; leur application a permis d’augmenter significativement l’activité biologique de la protéine libérée. La seconde stratégie consiste à moduler la matrice de copolymère PLGAP188-PLGA. La modification de ses propriétés physicochimiques (Mw, hydrophobie…) a permis d’accéder à la compréhension de la structure des microsphères et d’obtenir une libération continue.Le développement du procédé de fabrication des microsphères sans solvant toxique associe la technique du prilling avec le glycofurol comme solvant. Cette combinaison se heurte à de nombreux verrous technologiques. La mise au point du procédé a été réalisée à l’aide de plans d’expériences. Ils ont conduit à la production de particules grâce à la modélisation des propriétés physicochimiques du milieu de réception et à la prise en compte des différents paramètres du procédé.
Pharmacologically active microcarriers (PAM) have been developed as innovative tools for tissue regeneration. This microspherical platform provided an environment for the survival and the differentiation of stem cells through the release of encapsulated protein growth factor. To improve the therapeutic efficacy of the PAM, the microspheres have to (1) provide the full and sustained release of the protein (2) be formulated without halogenated solvent by a process with an easy scale-up. The protein release has been studied through two strategies. The first one was to look for a preservation of the biological activity of the protein during the release. A literature review highlighted protein additives. Some of them were incorporated into the microspheres and increased significantly the protein release. The second one was the modulation of the matrix copolymer PLGAP188-PLGA. The modification of its properties (MW,hydrophobicity) permitted to reach a continuous release and to understand the structure of the microspheres. The prilling technique and the use of glycofurol provide an easy transferable process without toxic solvent. Experimental designs were performed to overcome the technological barriers. Through the modeling the physicochemical properties of the reception medium and the study of the process parameters, the formulation has been improved to produce acceptable particles.
Advisors/Committee Members: Venier-Julienne, Marie-Claire (thesis director).
Subjects/Keywords: Microsphère; Protéine; Libération prolongée; Prilling; Plga; Poloxamère; Hsp27; Héparine; Microsphere; Protein; Drug Delivery; Prilling; Plga; Poloxamer; Hsp27; Heparin; 610
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APA (6th Edition):
Violet, F. (2015). Développement d’une protéine à libération prolongée, mise au point du procédé d’encapsulation sans solvant halogéné et optimisation du profil de libération. : Development of microencapsulation process without toxic solvent, application to sustained protein release. (Doctoral Dissertation). Angers. Retrieved from http://www.theses.fr/2015ANGE0040
Chicago Manual of Style (16th Edition):
Violet, Fabien. “Développement d’une protéine à libération prolongée, mise au point du procédé d’encapsulation sans solvant halogéné et optimisation du profil de libération. : Development of microencapsulation process without toxic solvent, application to sustained protein release.” 2015. Doctoral Dissertation, Angers. Accessed March 04, 2021.
http://www.theses.fr/2015ANGE0040.
MLA Handbook (7th Edition):
Violet, Fabien. “Développement d’une protéine à libération prolongée, mise au point du procédé d’encapsulation sans solvant halogéné et optimisation du profil de libération. : Development of microencapsulation process without toxic solvent, application to sustained protein release.” 2015. Web. 04 Mar 2021.
Vancouver:
Violet F. Développement d’une protéine à libération prolongée, mise au point du procédé d’encapsulation sans solvant halogéné et optimisation du profil de libération. : Development of microencapsulation process without toxic solvent, application to sustained protein release. [Internet] [Doctoral dissertation]. Angers; 2015. [cited 2021 Mar 04].
Available from: http://www.theses.fr/2015ANGE0040.
Council of Science Editors:
Violet F. Développement d’une protéine à libération prolongée, mise au point du procédé d’encapsulation sans solvant halogéné et optimisation du profil de libération. : Development of microencapsulation process without toxic solvent, application to sustained protein release. [Doctoral Dissertation]. Angers; 2015. Available from: http://www.theses.fr/2015ANGE0040
30.
Ben Azzouz, Seifeddine.
Libération contrôlée d'un neuroleptique par voie orale en utilisant des capsules hybrides PLGA-PEG / Alginate/ : Controlled releaseof antipsychotic by oral route using PLGA-PEG/Alginate hybrid capsules.
Degree: Docteur es, Chimie, 2017, Sorbonne Paris Cité
URL: http://www.theses.fr/2017USPCC116
► Actuellement les traitements thérapeutiques pour soigner la schizophrénie, par voie intraveineuse ou orale, ne sont qu’en partie efficaces et associés généralement à des effets extrapyramidaux…
(more)
▼ Actuellement les traitements thérapeutiques pour soigner la schizophrénie, par voie intraveineuse ou orale, ne sont qu’en partie efficaces et associés généralement à des effets extrapyramidaux souvent dangereux et très gênants pour les patients. Afin d’augmenter l’efficacité du traitement toute en neutralisant les effets indésirables, ce travail a eu comme objectif de concevoir des capsules composites (PLGA-PEG / alginate) destinées à être administrées par voie orale et capables de libérer localement, de façon spécifique et contrôlée, le neuroleptique halopéridol dans le cerveau. L’optimisation du protocole de synthèse a permis d’obtenir de façon reproductible des nanocapsules de PLGA poreuses monodisperses et peu agrégées, possédant un diamètre hydrodynamique moyen inférieur à 80 nm et une bonne stabilité en solution aqueuse. Une fois fonctionnalisées avec le Poly (éthylène glycol) diamine, des études in vitro ont montré la faible toxicité de ces nanoparticules furtives ainsi que leur capacité à encapsuler une quantité satisfaisante d’halopéridol et de libérer ce principe actif sur une durée d’un mois avec un faible effet « burst ». L’incorporation des nanoparticules pégylées dans des matrices préparées à haute concentration d’alginate et de 100 % CaCl2 a permis d’obtenir des billes nanocomposites possédants une meilleure stabilité à la sortie du milieu gastrique simulé et persistent environ 30 minutes en milieu intestinal simulé. Enfin des études in vivo préliminaires sur des souris adultes utilisant des nanoparticules injectées et des billes nanocomposites ingérées ont démontré l’efficacité de ces systèmes à délivrer l’halopéridol au cerveau.
Currently therapeutic treatments for schizophrenia, intravenously or orally, are only partially effective and generally associated with extrapyramidal effects often dangerous and very troublesome for patients. In order, to increase the treatment efficiency by neutralizing any side effects the aim of this work was to design composite capsules (PLGA-PEG / alginate) intended to be administered by way oral and able to release locally, in a specific and controlled way, the neuroleptic “haloperidol” in the brain. The optimization of the protocol of synthesis allowed to obtain in a reproducible way of the nanocapsules of monodisperse and not very aggregate porous PLGA, having an average hydrodynamic diameter lower than 80 Nm and a good stability in aqueous solution. Once functionalized with Poly (ethylene glycol) diamine, in vitro studies showed the low toxicity of these furtive nanoparticles as well as their ability to encapsulate a satisfactory amount of haloperidol and release this active principle over a period of one month with a low burst effect. The incorporation of the PEGylated nanoparticles in matrices prepared with a high concentration of alginate and 100% CaCl2 made it possible to obtain nanocomposite beads having a better stability at the exit from the simulated gastric medium and persist approximately 30 minutes in simulated intestinal medium. Finally, preliminary…
Advisors/Committee Members: Brayner, Roberta (thesis director), Coradin, Thibaud (thesis director).
Subjects/Keywords: Nanoparticules de PLGA-PEG; Alginate; Méthode double émulsion- évaporation du Solvant; PLGA-PEG nanoparticles; Alginate; Double emulsion- solvent evaporation technique
Record Details
Similar Records
Cite
Share »
Record Details
Similar Records
Cite
« Share
❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Ben Azzouz, S. (2017). Libération contrôlée d'un neuroleptique par voie orale en utilisant des capsules hybrides PLGA-PEG / Alginate/ : Controlled releaseof antipsychotic by oral route using PLGA-PEG/Alginate hybrid capsules. (Doctoral Dissertation). Sorbonne Paris Cité. Retrieved from http://www.theses.fr/2017USPCC116
Chicago Manual of Style (16th Edition):
Ben Azzouz, Seifeddine. “Libération contrôlée d'un neuroleptique par voie orale en utilisant des capsules hybrides PLGA-PEG / Alginate/ : Controlled releaseof antipsychotic by oral route using PLGA-PEG/Alginate hybrid capsules.” 2017. Doctoral Dissertation, Sorbonne Paris Cité. Accessed March 04, 2021.
http://www.theses.fr/2017USPCC116.
MLA Handbook (7th Edition):
Ben Azzouz, Seifeddine. “Libération contrôlée d'un neuroleptique par voie orale en utilisant des capsules hybrides PLGA-PEG / Alginate/ : Controlled releaseof antipsychotic by oral route using PLGA-PEG/Alginate hybrid capsules.” 2017. Web. 04 Mar 2021.
Vancouver:
Ben Azzouz S. Libération contrôlée d'un neuroleptique par voie orale en utilisant des capsules hybrides PLGA-PEG / Alginate/ : Controlled releaseof antipsychotic by oral route using PLGA-PEG/Alginate hybrid capsules. [Internet] [Doctoral dissertation]. Sorbonne Paris Cité; 2017. [cited 2021 Mar 04].
Available from: http://www.theses.fr/2017USPCC116.
Council of Science Editors:
Ben Azzouz S. Libération contrôlée d'un neuroleptique par voie orale en utilisant des capsules hybrides PLGA-PEG / Alginate/ : Controlled releaseof antipsychotic by oral route using PLGA-PEG/Alginate hybrid capsules. [Doctoral Dissertation]. Sorbonne Paris Cité; 2017. Available from: http://www.theses.fr/2017USPCC116
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Open Access Theses and Dissertations