subject:(PLGA)

NSYSU

1. Chi, Yi-Chun. A Comparison of PLGA and Titanium Plates When Used as a Fixation Material in Fractured Femurs of Diabetic Rats.

Degree: Master, Biological Sciences, 2010, NSYSU

URL: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0817110-020857

► Titanium bone plates and screws are commonly used in oral and maxillofacial surgery for internal fixation. Although titanium plate is good for fixation of bone… (more)

▼ Titanium bone plates and screws are commonly used in oral and maxillofacial surgery for internal fixation. Although titanium plate is good for fixation of bone fractures, there are still somemany disadvantages of the material. Biodegradable materials are considered to be good alternatives for treatment of the facial bone fractures. Poor wound healing in diabetic animals and human was well known. The aims of the studies were to compare the strength and tissue reaction of the poly lactic-co-glycolic acid ï¼PLGAï¼ with titanium plates used as fixation material in fractured-femora of diabetic and normal animals. The results of the studies show that the wounds of the control group healed quite well and there was no discharge around the wound. However, bullas formation was found in all the diabetic wounds fixed with PLGA. There was fistula with pus and gas discharge around the bullas. In the control group, the diameter of the PLGA-fixed femur was larger than the titanium-fixed femura. There was a large amount of callus formation around the PLGA-fixed fracture femur. At the 20th day, the defects of both groups of the PLGA treated were not healed. A large amount of new bone formation in normal titanium-treated rats was found, while in diabetic rats, mainly a fibrous tissue reaction. At the 40th day, the diabetic PLGA-fixed femora were poorly healed with a large amount of fibrous tissue. Similarly, after 40 days in the titanium-fixed femora of diabetic rats, extensive fibrosis of the defect was also present. In normal PLGA-fixed fractured femora, there was a large amount of cartilage present around the defect. Most of the normal PLGA-fixed fractured femora were distorted in shape. At the 40th day, lamellar bone formation was found around the screw in normal titanium-fixed femora and the defect was healed. In conclusion, the PLGA plate is not suitable for fixation in the fractured-femora of the rats in either normal or diabetic groups when compared with those from titanium plate treat groups. It is because of less strength of the PLGA plate and the material easily cause tissue reaction in the rat which result in the formation of large amount of fibrous tissue around the PLGA screw instead of bone. Advisors/Committee Members: Keng-Lian OU (chair), Yih-Chuen Shyng (committee member), Chau-Hsiang Wang (chair), David Chao (committee member).

Subjects/Keywords: Diabetic; PLGA

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APA (6^th Edition):

Chi, Y. (2010). A Comparison of PLGA and Titanium Plates When Used as a Fixation Material in Fractured Femurs of Diabetic Rats. (Thesis). NSYSU. Retrieved from http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0817110-020857

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Chi, Yi-Chun. “A Comparison of PLGA and Titanium Plates When Used as a Fixation Material in Fractured Femurs of Diabetic Rats.” 2010. Thesis, NSYSU. Accessed October 15, 2019. http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0817110-020857.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Chi, Yi-Chun. “A Comparison of PLGA and Titanium Plates When Used as a Fixation Material in Fractured Femurs of Diabetic Rats.” 2010. Web. 15 Oct 2019.

Vancouver:

Chi Y. A Comparison of PLGA and Titanium Plates When Used as a Fixation Material in Fractured Femurs of Diabetic Rats. [Internet] [Thesis]. NSYSU; 2010. [cited 2019 Oct 15]. Available from: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0817110-020857.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Chi Y. A Comparison of PLGA and Titanium Plates When Used as a Fixation Material in Fractured Femurs of Diabetic Rats. [Thesis]. NSYSU; 2010. Available from: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0817110-020857

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

2. Thuane Castro Frabel do Nascimento. DESENVOLVIMENTO E AVALIAÇÃO FARMACOCINÉTICA DE NANOPARTÍCULAS DE PLGA E PLGA-PEG CONTENDO CURCUMINA.

Degree: 2011, UNICENTRO – Universidade Estadual do Centro Oeste

URL: http://tede.unicentro.br/tde_busca/arquivo.php?codArquivo=120

►

As nanopartículas poliméricas apresentam grande importância na área farmacêutica em virtude de serem sistemas coloidais que possuem interessantes propriedades físicoquímicas, tais como o tamanho reduzido,… (more)

▼

As nanopartículas poliméricas apresentam grande importância na área farmacêutica em virtude de serem sistemas coloidais que possuem interessantes propriedades físicoquímicas, tais como o tamanho reduzido, a ampla área superficial, carga superficial, que as tornam eficientes sistemas para aplicação na liberação controlada de fármacos. A curcumina é um pigmento amarelo presente na Curcuma longa que possui baixa toxicidade e uma ampla faixa de atividades farmacológicas, estando entre os mais promissores e eficazes agentes quimiopreventivos e/ou antitumorais. Porém o seu uso terapêutico tem sido limitado devido a sua baixa solubilidade aquosa, sua alta velocidade de decomposição em pH neutro ou básico, além do rápido metabolismo e eliminação sistêmica, resultando em baixa biodisponibilidade. Neste trabalho obteve-se nanopartículas de ácido poli-(láctico-co-glicólico) (PLGA) e de blendas de PLGA com polietilenoglicol (PEG) contendo curcumina através da técnica de emulsificaçãoevaporação do solvente, com o objetivo de melhorar suas propriedades farmacocinéticas. Após a validação de um método por cromatografia líquida de alta eficiência (CLAE) para a quantificação da curcumina, as nanopartículas foram avaliadas quanto ao diâmetro médio e eficiência de encapsulação. Ambas as formulações obtiveram eficiência de encapsulação superior a 75% e o diâmetro médio não foi superior a 200 nm. O estudo de liberação in vitro mostrou que as nanopartículas sustentam a liberação da curcumina, e que a presença do PEG na formulação contribui para o aumento na velocidade de liberação da curcumina. Um método por cromatografia líquida acoplada a espectrometria de massas foi desenvolvido e validado e se mostrou altamente sensível, reprodutível e específico para análise de curcumina em plasma de rato. Após administração oral em ratos, as formulações de nanopartículas de PLGA e ii blendas de PLGA-PEG foram capazes de manter uma liberação sustentada da curcumina, com resultados significativamente diferentes entre as formulações. As nanopartículas de PLGA e PLGA-PEG aumentaram o tempo de meia vida da curcumina em aproximadamente 4 e 6 h, respectivamente. Comparando-se com a suspensão aquosa de curcumina, o pico máximo de concentração plasmática da curcumina a partir das nanopartículas de PLGA e PLGA-PEG foi 2,9 e 7,4 vezes superior, respectivamente. A distribuição e o metabolismo da curcumina foi reduzido quando carreada pelas nanopartículas, principalmente pelas nanopartículas de PLGA-PEG. A biodisponibilidade da curcumina encapsulada em nanopartículas de PLGA-PEG foi 3,5 vezes superior em relação a encapsulada em nanopartículas de PLGA. Comparado com a suspensão aquosa de curcumina, as nanopartículas de PLGA e PLGA-PEG aumentaram a biodisponibilidade em 15,6 e 55,4 vezes, respectivamente. Estes resultados sugerem que nanopartículas de PLGA e principalmente de PLGA-PEG são promissores carreadores de curcumina para administração oral.

The polymeric nanoparticles present great importance in the pharmaceutical field due to be colloidal systems,…

Advisors/Committee Members: Rubiana Mara Mainardes.

Subjects/Keywords: Curcumina; Biodisponibilidade; LC-MS/MS; Nanopartículas, PLGA; PLGA-PEG; Curcumin; Bioavailability; LC-MS/MS; Nanoparticles; PLGA; PLGA-PEG; FARMACIA

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Nascimento, T. C. F. d. (2011). DESENVOLVIMENTO E AVALIAÇÃO FARMACOCINÉTICA DE NANOPARTÍCULAS DE PLGA E PLGA-PEG CONTENDO CURCUMINA. (Thesis). UNICENTRO – Universidade Estadual do Centro Oeste. Retrieved from http://tede.unicentro.br/tde_busca/arquivo.php?codArquivo=120

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Nascimento, Thuane Castro Frabel do. “DESENVOLVIMENTO E AVALIAÇÃO FARMACOCINÉTICA DE NANOPARTÍCULAS DE PLGA E PLGA-PEG CONTENDO CURCUMINA.” 2011. Thesis, UNICENTRO – Universidade Estadual do Centro Oeste. Accessed October 15, 2019. http://tede.unicentro.br/tde_busca/arquivo.php?codArquivo=120.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Nascimento, Thuane Castro Frabel do. “DESENVOLVIMENTO E AVALIAÇÃO FARMACOCINÉTICA DE NANOPARTÍCULAS DE PLGA E PLGA-PEG CONTENDO CURCUMINA.” 2011. Web. 15 Oct 2019.

Vancouver:

Nascimento TCFd. DESENVOLVIMENTO E AVALIAÇÃO FARMACOCINÉTICA DE NANOPARTÍCULAS DE PLGA E PLGA-PEG CONTENDO CURCUMINA. [Internet] [Thesis]. UNICENTRO – Universidade Estadual do Centro Oeste; 2011. [cited 2019 Oct 15]. Available from: http://tede.unicentro.br/tde_busca/arquivo.php?codArquivo=120.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Nascimento TCFd. DESENVOLVIMENTO E AVALIAÇÃO FARMACOCINÉTICA DE NANOPARTÍCULAS DE PLGA E PLGA-PEG CONTENDO CURCUMINA. [Thesis]. UNICENTRO – Universidade Estadual do Centro Oeste; 2011. Available from: http://tede.unicentro.br/tde_busca/arquivo.php?codArquivo=120

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Penn State University

3. Kuo, Chung-fan. Development Of A New Nano-scale Vehicle For Delivery Of Curcumin In Biological Systems.

Degree: MS, Bioengineering, 2014, Penn State University

URL: https://etda.libraries.psu.edu/catalog/22459

► Curcumin, a natural compound extracted from turmeric, has anti-inflammatory, anti-oxidant and neuroprotective properties that make it an attractive therapeutic agent for treatment of disesaes such… (more)

Subjects/Keywords: Lipid-coated PLGA nanoparticles; Curcumin

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APA (6^th Edition):

Kuo, C. (2014). Development Of A New Nano-scale Vehicle For Delivery Of Curcumin In Biological Systems. (Masters Thesis). Penn State University. Retrieved from https://etda.libraries.psu.edu/catalog/22459

Chicago Manual of Style (16^th Edition):

Kuo, Chung-fan. “Development Of A New Nano-scale Vehicle For Delivery Of Curcumin In Biological Systems.” 2014. Masters Thesis, Penn State University. Accessed October 15, 2019. https://etda.libraries.psu.edu/catalog/22459.

MLA Handbook (7^th Edition):

Kuo, Chung-fan. “Development Of A New Nano-scale Vehicle For Delivery Of Curcumin In Biological Systems.” 2014. Web. 15 Oct 2019.

Vancouver:

Kuo C. Development Of A New Nano-scale Vehicle For Delivery Of Curcumin In Biological Systems. [Internet] [Masters thesis]. Penn State University; 2014. [cited 2019 Oct 15]. Available from: https://etda.libraries.psu.edu/catalog/22459.

Council of Science Editors:

Kuo C. Development Of A New Nano-scale Vehicle For Delivery Of Curcumin In Biological Systems. [Masters Thesis]. Penn State University; 2014. Available from: https://etda.libraries.psu.edu/catalog/22459

Louisiana State University

4. Whaley, Meocha. Physical Stability of Poly (lactic-co-glycolic acid) and PLGA/Chitosan Nanoparticles and Chemical Stability of Entrapped Alpha-Tocopherol and Lutein.

Degree: MSBAE, Engineering, 2014, Louisiana State University

URL: etd-07132014-234534 ; https://digitalcommons.lsu.edu/gradschool_theses/544

► The published benefits of polymeric nanoparticles as a system for antioxidant delivery have encompassed topics of improved oral delivery, bioavailability, and modified release to… (more)

Subjects/Keywords: nanoparticles; PLGA; chitosan; stability

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APA (6^th Edition):

Whaley, M. (2014). Physical Stability of Poly (lactic-co-glycolic acid) and PLGA/Chitosan Nanoparticles and Chemical Stability of Entrapped Alpha-Tocopherol and Lutein. (Masters Thesis). Louisiana State University. Retrieved from etd-07132014-234534 ; https://digitalcommons.lsu.edu/gradschool_theses/544

Chicago Manual of Style (16^th Edition):

Whaley, Meocha. “Physical Stability of Poly (lactic-co-glycolic acid) and PLGA/Chitosan Nanoparticles and Chemical Stability of Entrapped Alpha-Tocopherol and Lutein.” 2014. Masters Thesis, Louisiana State University. Accessed October 15, 2019. etd-07132014-234534 ; https://digitalcommons.lsu.edu/gradschool_theses/544.

MLA Handbook (7^th Edition):

Whaley, Meocha. “Physical Stability of Poly (lactic-co-glycolic acid) and PLGA/Chitosan Nanoparticles and Chemical Stability of Entrapped Alpha-Tocopherol and Lutein.” 2014. Web. 15 Oct 2019.

Vancouver:

Whaley M. Physical Stability of Poly (lactic-co-glycolic acid) and PLGA/Chitosan Nanoparticles and Chemical Stability of Entrapped Alpha-Tocopherol and Lutein. [Internet] [Masters thesis]. Louisiana State University; 2014. [cited 2019 Oct 15]. Available from: etd-07132014-234534 ; https://digitalcommons.lsu.edu/gradschool_theses/544.

Council of Science Editors:

Whaley M. Physical Stability of Poly (lactic-co-glycolic acid) and PLGA/Chitosan Nanoparticles and Chemical Stability of Entrapped Alpha-Tocopherol and Lutein. [Masters Thesis]. Louisiana State University; 2014. Available from: etd-07132014-234534 ; https://digitalcommons.lsu.edu/gradschool_theses/544

Louisiana State University

5. Simon, Lacey Caroline. Bioavailability of Alpha-Tocopherol Entrapped in Poly (lactide-co-glycolide) (PLGA) and PLGA-Chitosan Nanoparticles.

Degree: MSBAE, Engineering, 2014, Louisiana State University

URL: etd-04112014-185904 ; https://digitalcommons.lsu.edu/gradschool_theses/2290

► Alpha-tocopherol (áT) is a hydrophobic, antioxidant molecule shown to prevent or retard the effects of free radicals associated with diseases. It is hypothesized that the… (more)

▼ Alpha-tocopherol (áT) is a hydrophobic, antioxidant molecule shown to prevent or retard the effects of free radicals associated with diseases. It is hypothesized that the bioavailability of áT can be improved when entrapped in PLGA (Poly lactide-co-glycolide) and PLGA-Chi (PLGA-Chitosan) nanoparticles and that the mucoadhesive properties of chitosan may enhance absorption more than PLGA alone. PLGA and PLGA-Chi NPs synthesized with PVA as surfactant were characterized by measuring entrapment efficiency, size, PDI, and zeta potential when suspended in DI water. Nanoparticle physical stability was evaluated via NP exposure to environmental pH ranging from 2.5-9. Chemical stability of entrapped áT was also investigated by exposing particles to simulated GI (gastro-intestinal) environments for 48 hours, and release kinetics were measured in these conditions for 72 hours. Pharmacokinetic studies were conducted by administering PLGA (áT) NPs, PLGA-Chi (áT) NPs, and free áT in a flour, water, and corn oil slurry via oral gavage in rats. Blood samples were collected over 72 hours. Entrapment efficiency was 95.4 ± 9.85%, for PLGA NPs and 77.95 ± 1.51% for PLGA-Chi NPs. The size and zeta potential of the two particle systems were 97.87 ± 2.63 nm and -36.2 ± 1.31 mV for PLGA(áT) NPs, and 134 ± 2.05 nm and 38.0 ± 2.90 mV for PLGA-Chi (áT) nanoparticles in DI water. PLGA(áT) NPs showed a change in size of only 4 nm in the pH range of 2.5-9, while PLGA-Chi(áT) nanoparticles revealed a 46 nm change in size in pH 2.5-9. Entrapped áT showed no degradation in GI conditions over 48 hours. Release kinetics also showed no release of the áT from either NP system over 72 hours. Bioavailability of nanodelivered áT was improved as indicated compared to the free áT up to 170% and 121% for PLGA and PLGA-Chi NPs, respectively. These findings revealed that both delivery systems increased bioavailability of áT, but chitosan did not improve uptake of áT, as hypothesized.

Subjects/Keywords: Alpha-tocopherol; Bioavailability; Nanoparticles; PLGA

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APA (6^th Edition):

Simon, L. C. (2014). Bioavailability of Alpha-Tocopherol Entrapped in Poly (lactide-co-glycolide) (PLGA) and PLGA-Chitosan Nanoparticles. (Masters Thesis). Louisiana State University. Retrieved from etd-04112014-185904 ; https://digitalcommons.lsu.edu/gradschool_theses/2290

Chicago Manual of Style (16^th Edition):

Simon, Lacey Caroline. “Bioavailability of Alpha-Tocopherol Entrapped in Poly (lactide-co-glycolide) (PLGA) and PLGA-Chitosan Nanoparticles.” 2014. Masters Thesis, Louisiana State University. Accessed October 15, 2019. etd-04112014-185904 ; https://digitalcommons.lsu.edu/gradschool_theses/2290.

MLA Handbook (7^th Edition):

Simon, Lacey Caroline. “Bioavailability of Alpha-Tocopherol Entrapped in Poly (lactide-co-glycolide) (PLGA) and PLGA-Chitosan Nanoparticles.” 2014. Web. 15 Oct 2019.

Vancouver:

Simon LC. Bioavailability of Alpha-Tocopherol Entrapped in Poly (lactide-co-glycolide) (PLGA) and PLGA-Chitosan Nanoparticles. [Internet] [Masters thesis]. Louisiana State University; 2014. [cited 2019 Oct 15]. Available from: etd-04112014-185904 ; https://digitalcommons.lsu.edu/gradschool_theses/2290.

Council of Science Editors:

Simon LC. Bioavailability of Alpha-Tocopherol Entrapped in Poly (lactide-co-glycolide) (PLGA) and PLGA-Chitosan Nanoparticles. [Masters Thesis]. Louisiana State University; 2014. Available from: etd-04112014-185904 ; https://digitalcommons.lsu.edu/gradschool_theses/2290

University of Saskatchewan

6. Jahan, Sheikh Tasnim 1984-. DESIGN AND DEVELOPMENT OF CD205 TARGETED PLGA NANOPARTICLES AND EVALUATION OF ANTIGEN SPECIFIC IMMUNE RESPONSES.

Degree: 2017, University of Saskatchewan

URL: http://hdl.handle.net/10388/7881

► Stimulation of a patient’s immune system to fight cancer is the underlying mechanism of immunotherapy. Cancer immunotherapy manipulates the dendritic cells (DCs) to identify the… (more)

▼ Stimulation of a patient’s immune system to fight cancer is the underlying mechanism of immunotherapy. Cancer immunotherapy manipulates the dendritic cells (DCs) to identify the non-self present in the immunosuppressive microenvironment. This vaccination strategy based on nanoparticulate drug delivery system has the potential to treat cancer through packaging of therapeutic cargoes and delivering them to target immune cells (DCs). FDA approved poly-(D, L-lactic-co-glycolide) is approved for use in human due to its widely accepted properties such as low immunogenicity, minimal toxicity, biocompatibility and biodegradability. The goal of this project is to develop an understanding of a comprehensive relationship between nanoparticle (NP) structure and activity; and address the important requirements of NP structure and chemistry to selectively target specific markers. Plain NPs were prepared by emulsification solvent evaporation method with number of preparation variables. Double emulsification solvent evaporation method was used to prepare ovalbumin (OVA) and/or adjuvant loaded NPs. The DC targeting ligand (anti-CD205 monoclonal antibody) was attached to the NPs through two methods: covalent binding in presence of spacer molecule and physical adsorption method. Infra-red (IR) spectroscopy was performed to ensure the structural modification of NPs. Formulations were evaluated in respect to particle size, polydispersity index, zeta potential, surface display, cytotoxicity assay, OVA release studies, structural integrity of OVA in formulations, DC uptake study, DC maturation study, T cell proliferation study, estimation of total IgG and cytokine secretion profile. Results indicated that different formulation groups of NPs with different viscosity grades had desirable physicochemical properties. In case of the ligand (anti-CD205 antibody) conjugated NP’s spectrum, there was presence of amide-I vibrations resulted from C=O stretching vibration near 1610 cm-1 and N-H stretching of high intensity between 3310 to 3250 cm-1. These characteristic IR peak reflects the antibody conjugation with the NPs. DC uptake study shows when ligand was adsorbed onto the surface, these NPs were better uptaken compared to covalently attached formulations. No significant correlation was observed in uptake due to change of polymer viscosity and type. Highest expression of markers CD40, CD86 and MHCII molecules were observed with adjuvant (monophosphoryl lipid A)-antigen loaded targeted NPs. Though, high viscosity grade polymers (ester or COOH terminated) had higher OVA loading, they expressed lower percentage of markers compared to low viscosity formulations. These could be attributed to the release mechanism of the respective PLGA NP formulation. In addition, sufficient secretion of T helper cell 1 (Th1) and Th2 cytokines was observed. This confirmed the maturation of DCs as well as activation of the T cells. The T cell proliferation study confirmed the proliferation of cells in-vitro for both wild type balb/c and TCR transgenic (OT1) mice.… Advisors/Committee Members: Nazarali, Adil, Yang, Jian, Xiang, Jim, Blackburn, David, Haddadi, Azita.

Subjects/Keywords: PLGA Nanoparticle; Dendritic cells; Vaccine

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APA (6^th Edition):

Jahan, S. T. 1. (2017). DESIGN AND DEVELOPMENT OF CD205 TARGETED PLGA NANOPARTICLES AND EVALUATION OF ANTIGEN SPECIFIC IMMUNE RESPONSES. (Thesis). University of Saskatchewan. Retrieved from http://hdl.handle.net/10388/7881

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Jahan, Sheikh Tasnim 1984-. “DESIGN AND DEVELOPMENT OF CD205 TARGETED PLGA NANOPARTICLES AND EVALUATION OF ANTIGEN SPECIFIC IMMUNE RESPONSES.” 2017. Thesis, University of Saskatchewan. Accessed October 15, 2019. http://hdl.handle.net/10388/7881.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Jahan, Sheikh Tasnim 1984-. “DESIGN AND DEVELOPMENT OF CD205 TARGETED PLGA NANOPARTICLES AND EVALUATION OF ANTIGEN SPECIFIC IMMUNE RESPONSES.” 2017. Web. 15 Oct 2019.

Vancouver:

Jahan ST1. DESIGN AND DEVELOPMENT OF CD205 TARGETED PLGA NANOPARTICLES AND EVALUATION OF ANTIGEN SPECIFIC IMMUNE RESPONSES. [Internet] [Thesis]. University of Saskatchewan; 2017. [cited 2019 Oct 15]. Available from: http://hdl.handle.net/10388/7881.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Jahan ST1. DESIGN AND DEVELOPMENT OF CD205 TARGETED PLGA NANOPARTICLES AND EVALUATION OF ANTIGEN SPECIFIC IMMUNE RESPONSES. [Thesis]. University of Saskatchewan; 2017. Available from: http://hdl.handle.net/10388/7881

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Oklahoma State University

7. Sharma, Munish. Development of a Novel Nanotechnology-Based Ophthalmic Drug Delivery Device.

Degree: School of Chemical Engineering, 2010, Oklahoma State University

URL: http://hdl.handle.net/11244/9665

► Topical delivery by eye drops, which accounts for approximately 90% of all ophthalmic formulations, is extremely inefficient. Only approximately 5% of the drug applied as… (more)

▼ Topical delivery by eye drops, which accounts for approximately 90% of all ophthalmic formulations, is extremely inefficient. Only approximately 5% of the drug applied as drops penetrates the outer layer of the eye and reaches the target, while the rest is lost due to tear drainage. A number of conditions like glaucoma, conjunctivitis, and proliferative retinopathy need a sustained release of drug inside the eye for drug to be therapeutically effective. In addition to eye drops, other ocular drug delivery systems include drug-loaded liposomes or nanoparticles and drug-loaded contact lenses (hydrogels). Particle drug delivery systems also have difficulty penetrating the outer layer of the eye and can be lost before the drug can be released. Contact lens delivery systems often show a burst release with poor release over long periods of time. To improve sustain delivery of drugs to the eye, we have designed a novel system that includes drug-loaded nanoparticles suspended within a thin, membrane that can be attached to a standard commercial-grade contact lens for support. The lens system will provide constant contact with the eye's surface and the particles will supply a continuous release of medication, resulting in more drug reaching the target. First, PLGA nanoparticles, synthesized by the emulsion solvent evaporation technique, are loaded with the hydrophobic drug lidocaine. Next, the nanoparticles are loaded within a thin, collagen membrane that can be stored dried, and later rehydrated and attached to a contact lens for delivery to the eye. Drug loading and drug release rates must be controlled within the novel delivery system in order to ensure that therapeutic levels of the drug are delivered safely to the eye. The PLGA nanoparticles were found to be irregular in shape, 247 nm in diameter, and contained 5.17 wt% of lidocaine. The nanoparticles loaded into the collagen membrane did not have a significant effect on light transmittance through the membrane, compared to a commercially available contact lens. For initial drug release in the first 48 hours, the complete lens system, nanoparticles only, and collagen membrane only released 21.8%, 53.0% and 7.6% of the initial drug loading, respectively. For the first 400 hours tested, the complete lens system, nanoparticles only, and collagen membrane only released 20.8%, 63.9 and 7.0% of the initial drug loading, respectively.

Subjects/Keywords: collagen; gc; lidocaine; loading; plga

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APA (6^th Edition):

Sharma, M. (2010). Development of a Novel Nanotechnology-Based Ophthalmic Drug Delivery Device. (Thesis). Oklahoma State University. Retrieved from http://hdl.handle.net/11244/9665

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Sharma, Munish. “Development of a Novel Nanotechnology-Based Ophthalmic Drug Delivery Device.” 2010. Thesis, Oklahoma State University. Accessed October 15, 2019. http://hdl.handle.net/11244/9665.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Sharma, Munish. “Development of a Novel Nanotechnology-Based Ophthalmic Drug Delivery Device.” 2010. Web. 15 Oct 2019.

Vancouver:

Sharma M. Development of a Novel Nanotechnology-Based Ophthalmic Drug Delivery Device. [Internet] [Thesis]. Oklahoma State University; 2010. [cited 2019 Oct 15]. Available from: http://hdl.handle.net/11244/9665.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Sharma M. Development of a Novel Nanotechnology-Based Ophthalmic Drug Delivery Device. [Thesis]. Oklahoma State University; 2010. Available from: http://hdl.handle.net/11244/9665

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Missouri – Kansas City

8. Boddu, Sai Hanuman Sagar, 1981-. Fomulation and Delivery of Drugs for Macular Edema and Retinoblastoma; Synthesis and In Vitro Characterization of Doxorubicin Loaded Surface Modified Nanoparticles Using PLGA-PEG-PLGA Polymer .

Degree: 2011, University of Missouri – Kansas City

URL: http://hdl.handle.net/10355/9588

► Macular edema (ME) is caused by central extravascular swelling of the macula resulting in a significant loss of visual activity. Corticosteroids are widely used in… (more)

▼ Macular edema (ME) is caused by central extravascular swelling of the macula resulting in a significant loss of visual activity. Corticosteroids are widely used in the treatment of ME. In this project, nanoparticles of dexamethasone (DEX), hydrocortisone acetate (HA) and prednisolone acetate (PA) were developed and characterized for size, shape, polydispersity, and in vitro release. Further the effect of PLGA-PEG-PLGA thermosensitive gel on the release mechanisms of steroids from PLGA nanoparticles was also studied. Release from nanoparticles suspended in thermosensitive gels followed zero-order kinetics with no apparent burst effect. Ex vivo permeability studies further confirmed sustained release of DEX from nanoparticles suspended in thermosensitive gels. Retinoblastoma (RB) represents a common form of intraocular malignancy affecting the retina. Recent work has explored the systemic/intravitreal administration of topotecan, etoposide, carboplatin and vincristine for the treatment of RB. However, the current therapy is associated with non-specific toxicity and patient non-compliance. An attempt was made to develop and evaluate a novel folate receptor targeted and sustained drug delivery system for RB cells using doxorubicin (DOX) as a model drug. Uptake of DOX was approximately four times higher with DOX-loaded PLGA-PEG-FOL micelles (DOXM) than DOX in Y-79 cells over-expressing folate receptors. Dispersion of DOXM in PLGA-PEG-PLGA gel sustained drug release over a period of two weeks. Nanoparticles are colloidal particulate systems widely employed in targeted delivery of drugs to cancer cells. In this project, a novel strategy was proposed for the synthesis of folate conjugated nanoparticles for both hydrophilic and hydrophobic drug molecules using PLGA-PEG-FOL polymer. Nanoparticles were evaluated for the entrapment efficiency, morphology, particle size and in vitro release. Presence of folate on the nanoparticle surface was confirmed by using 1HNMR and TEM studies. Quantitative uptake and cell viability studies were carried out in folate receptor-positive ovarian cancer (SKOV3) cells. Folate conjugated nanoparticles (size ~200 nm) were successfully prepared using single (O/W) and double emulsion (W/O/W) methods. Folate conjugated nanoparticles exhibited higher uptake and cytotoxicity in SKOV3 cells in comparison with the pure DOX and unmodified nanoparticles. Advisors/Committee Members: Mitra, Ashim K., 1954- (advisor).

Subjects/Keywords: Targeted Delivery; PLGA-PEG-FOL

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Boddu, Sai Hanuman Sagar, 1. (2011). Fomulation and Delivery of Drugs for Macular Edema and Retinoblastoma; Synthesis and In Vitro Characterization of Doxorubicin Loaded Surface Modified Nanoparticles Using PLGA-PEG-PLGA Polymer . (Thesis). University of Missouri – Kansas City. Retrieved from http://hdl.handle.net/10355/9588

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Boddu, Sai Hanuman Sagar, 1981-. “Fomulation and Delivery of Drugs for Macular Edema and Retinoblastoma; Synthesis and In Vitro Characterization of Doxorubicin Loaded Surface Modified Nanoparticles Using PLGA-PEG-PLGA Polymer .” 2011. Thesis, University of Missouri – Kansas City. Accessed October 15, 2019. http://hdl.handle.net/10355/9588.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Boddu, Sai Hanuman Sagar, 1981-. “Fomulation and Delivery of Drugs for Macular Edema and Retinoblastoma; Synthesis and In Vitro Characterization of Doxorubicin Loaded Surface Modified Nanoparticles Using PLGA-PEG-PLGA Polymer .” 2011. Web. 15 Oct 2019.

Vancouver:

Boddu, Sai Hanuman Sagar 1. Fomulation and Delivery of Drugs for Macular Edema and Retinoblastoma; Synthesis and In Vitro Characterization of Doxorubicin Loaded Surface Modified Nanoparticles Using PLGA-PEG-PLGA Polymer . [Internet] [Thesis]. University of Missouri – Kansas City; 2011. [cited 2019 Oct 15]. Available from: http://hdl.handle.net/10355/9588.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Boddu, Sai Hanuman Sagar 1. Fomulation and Delivery of Drugs for Macular Edema and Retinoblastoma; Synthesis and In Vitro Characterization of Doxorubicin Loaded Surface Modified Nanoparticles Using PLGA-PEG-PLGA Polymer . [Thesis]. University of Missouri – Kansas City; 2011. Available from: http://hdl.handle.net/10355/9588

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

9. Renata Kely de Paulo Moura. Avaliação in vitro das características de liberação de indometacina a partir de dispositivos oculares implantáveis.

Degree: 2010, Universidade Federal da Paraíba

URL: http://bdtd.biblioteca.ufpb.br/tde_busca/arquivo.php?codArquivo=1226

► A anatomia e fisiologia do olho e suas barreiras protetoras representam um desafio para o desenvolvimento de sistemas de transporte de drogas oftálmicas efetivos. O… (more)

▼ A anatomia e fisiologia do olho e suas barreiras protetoras representam um desafio para o desenvolvimento de sistemas de transporte de drogas oftálmicas efetivos. O tratamento farmacológico de doenças oculares tem se limitado às formas convencionais de administração que não são satisfatórias para o tratamento de doenças que acometem o segmento posterior do bulbo do olho. O transporte de doses terapêuticas para os tecidos do segmento posterior do olho, que minimize os efeitos colaterais sistêmicos e locais, é o principal objetivo no tratamento de doenças oculares. Visando atingir este objetivo, estudos têm sido feitos no sentido de desenvolver novos sistemas de liberação de fármacos para o olho, entre estes estão os implantes. Esses implantes são preparados a partir de diferentes polímeros, os quais podem ser biodegradáveis ou não biodegradáveis. Os polímeros derivados dos ácidos lático e glicólico têm se revelado bastante promissores devido, principalmente, às suas características de biocompatibilidade e biodegradabilidade. Neste trabalho, dois diferentes implantes de indometacina formulados a partir do copolímero ácido lático / ácido glicólico (PLGA 50:50) e do polímero derivado do ácido D,L-lático (PLA), através de liofilização da mistura polímero-fármaco, foram fornecidos pela empresa 3T Biopolímeros (São Paulo - SP) e avaliados através de DSC, estudos de liberação in vitro usando aparato de dissolução e estudos de difusão através de esclera de coelho utilizando câmaras de Franz. Os resultados da validação do método CLAE para quantificação da indometacina apresentaram-se dentro dos limites estabelecidos pela legislação brasileira (Resolução RE 899, 2003, ANVISA). As análises de DSC mostraram a ausência aparente de interações químicas e físicas entre o fármaco e os polímeros, sendo sugerido que o desaparecimento do pico de fusão da indometacina tenha ocorrido pela amorfização do fármaco durante o processo de liofilização ou pela diluição do fármaco no polímero. Os estudos preliminares de liberação in vitro demonstraram um perfil trifásico para os implantes formulados com PLGA e um perfil bifásico para àqueles formulados com PLA. Os implantes formulados com PLGA promoveram uma liberação mais rápida da indometacina (103,64%) quando comparado com os implantes formulados com PLA (49,9%) durante os trinta dias de experimento. O perfil de liberação da indometacina foi determinado pela velocidade de degradação dos polímeros, que também determinou a difusão escleral da indometacina a partir do PLGA e do PLA (1,7 x 10-5 cm/s e 0,24 x 10-5 cm/s, respectivamente). Os estudos de difusão escleral mostraram que a esclera de coelho é permeável à indometacina e a microscopia de luz polarizada mostrou que a estrutura das fibras colágenas da esclera não foram significativamente alteradas durante o estudo de difusão nas câmaras de Franz. Os sistemas de liberação de fármacos avaliados foram capazes de liberar a indometacina de forma prolongada, servindo como um modelo para formulação de implantes de indometacina que podem ser… Advisors/Committee Members: Eduardo de Jesus Oliveira.

Subjects/Keywords: PLA; indomethacin; Ocular implants; Implantes oculares; PLGA; Indometacina; FARMACOLOGIA; PLA; PLGA

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Moura, R. K. d. P. (2010). Avaliação in vitro das características de liberação de indometacina a partir de dispositivos oculares implantáveis. (Thesis). Universidade Federal da Paraíba. Retrieved from http://bdtd.biblioteca.ufpb.br/tde_busca/arquivo.php?codArquivo=1226

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Moura, Renata Kely de Paulo. “Avaliação in vitro das características de liberação de indometacina a partir de dispositivos oculares implantáveis.” 2010. Thesis, Universidade Federal da Paraíba. Accessed October 15, 2019. http://bdtd.biblioteca.ufpb.br/tde_busca/arquivo.php?codArquivo=1226.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Moura, Renata Kely de Paulo. “Avaliação in vitro das características de liberação de indometacina a partir de dispositivos oculares implantáveis.” 2010. Web. 15 Oct 2019.

Vancouver:

Moura RKdP. Avaliação in vitro das características de liberação de indometacina a partir de dispositivos oculares implantáveis. [Internet] [Thesis]. Universidade Federal da Paraíba; 2010. [cited 2019 Oct 15]. Available from: http://bdtd.biblioteca.ufpb.br/tde_busca/arquivo.php?codArquivo=1226.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Moura RKdP. Avaliação in vitro das características de liberação de indometacina a partir de dispositivos oculares implantáveis. [Thesis]. Universidade Federal da Paraíba; 2010. Available from: http://bdtd.biblioteca.ufpb.br/tde_busca/arquivo.php?codArquivo=1226

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

10. Courant, Thomas. Nanoparticules incorporant des complexes inorganiques à visée diagnostique : Metal complexes-loaded nanoparticles for diagnostic and medical imaging.

Degree: Docteur es, Chimie, 2011, Reims

URL: http://www.theses.fr/2011REIMS005

►

L’objectif poursuivi au cours de ce travail est l’encapsulation de complexes métalliques au sein de nanoparticules biocompatibles, et ce pour des visées diagnostiques. Dans ce… (more)

▼

L’objectif poursuivi au cours de ce travail est l’encapsulation de complexes métalliques au sein de nanoparticules biocompatibles, et ce pour des visées diagnostiques. Dans ce but, un protocole de double émulsion-diffusion de solvant Wi/O/We, n’utilisant que des composés biocompatibles, a été mis au point et optimisé pour obtenir, de façon quantitative et reproductible, des nanoparticules de PLGA de diamètre compatible avec une injection par voie parentérale. Cette formulation a été employée avec succès pour l’encapsulation de complexes modèles de Cu(II). Les formulations optimales permettent d’obtenir des nanoparticules possédant des diamètres hydrodynamiques moyens inférieurs à 200 nm avec des efficacités d’encapsulation entre 20 et 25 %. L’utilisation de cette formulation pour l’encapsulation de chélates de gadolinium ne permet pas d’obtenir des rendements d’encapsulation satisfaisants. La modification du protocole vers une méthodologie Wi/O1/O2ne permet pas d’améliorer l’encapsulation et dénote l’absence d’affinités entre le polymère hydrophobe et les complexes hydrophiles. L’utilisation de nanoparticules composées d’une matrice hydrophile permet d’obtenir des taux de charges nettement supérieurs. Ceci conforte l’hypothèse selon laquelle les interactions entre le complexe et la matrice des nanoparticules jouent un rôle crucial pour l’encapsulation.

The goal of this work was to encapsulate metal complexes into biocompatible nanoparticles for diagnostics. To reach this purpose, a double emulsion-solvent diffusion Wi/O/We technique was optimized, using only biocompatible compounds. It allowed the obtention of PLGA nanoparticles that are compatible with parenteral injections in a reproducible and quantitative way. This formulation was successfully applied to encapsulate model Cu(II)complexes. Optimal formulations showed mean diameters below 200 nm with encapsulation yields in the 20-25 % range. The use of this formulation for gadolinium chelates did not lead to satisfactory encapsulation yields. Thereafter, a Wi/O1/O2 methodology was developed but could not allow to raise the encapsulation efficiencies. This point showed the lack of affinity between the hydrophobic polymer and the hydrophilic chelates. The use of nanoparticles made of an hydrophilic matrix showed a ten-fold increase in the drug loading efficiency. This confirms the hypothesis in which interactions between chelates and nanoparticle matrices play a crucial role for encapsulation.

Advisors/Committee Members: Chuburu, Françoise (thesis director), Andry, Marie-Christine (thesis director).

Subjects/Keywords: Encapsulation; Gadolinium; Agents de contraste.; PLGA; Encapsulation; Gadolinium; Contrast agents.; PLGA

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Courant, T. (2011). Nanoparticules incorporant des complexes inorganiques à visée diagnostique : Metal complexes-loaded nanoparticles for diagnostic and medical imaging. (Doctoral Dissertation). Reims. Retrieved from http://www.theses.fr/2011REIMS005

Chicago Manual of Style (16^th Edition):

Courant, Thomas. “Nanoparticules incorporant des complexes inorganiques à visée diagnostique : Metal complexes-loaded nanoparticles for diagnostic and medical imaging.” 2011. Doctoral Dissertation, Reims. Accessed October 15, 2019. http://www.theses.fr/2011REIMS005.

MLA Handbook (7^th Edition):

Courant, Thomas. “Nanoparticules incorporant des complexes inorganiques à visée diagnostique : Metal complexes-loaded nanoparticles for diagnostic and medical imaging.” 2011. Web. 15 Oct 2019.

Vancouver:

Courant T. Nanoparticules incorporant des complexes inorganiques à visée diagnostique : Metal complexes-loaded nanoparticles for diagnostic and medical imaging. [Internet] [Doctoral dissertation]. Reims; 2011. [cited 2019 Oct 15]. Available from: http://www.theses.fr/2011REIMS005.

Council of Science Editors:

Courant T. Nanoparticules incorporant des complexes inorganiques à visée diagnostique : Metal complexes-loaded nanoparticles for diagnostic and medical imaging. [Doctoral Dissertation]. Reims; 2011. Available from: http://www.theses.fr/2011REIMS005

University of Akron

11. Cheung, Maureen Elizabeth. A Swine Model for the Quantification of Pelvic Adhesions and the Encapsulation of Ketorolac Tromethamine for the Prevention of Adhesion Formation.

Degree: MSin Engineering, Chemical Engineering, 2010, University of Akron

URL: http://rave.ohiolink.edu/etdc/view?acc_num=akron1280274930

► Introduction: Adhesions following surgery represent a significant problem often resulting in pain, disability, and additional surgeries. Compounds are available for the prevention of postoperative… (more)

▼ Introduction: Adhesions following surgery represent a significant problem often resulting in pain, disability, and additional surgeries. Compounds are available for the prevention of postoperative adhesions, but effectiveness is difficult to assess; current models of adhesion comparison are limited to qualitative methods with much potential bias. Objectives: The objectives of the research performed were to create a quantitative model of adhesion strength assessment, to successfully encapsulate ketorolac tromethamine (KT) into poly(lactic-co-glycolic acid) microspheres, and to characterize of the microspheres for use in preventing adhesion formation. Quantitative Model Methods: The primary focus of this research was the creation of an adhesion complex that was suitable to quantitative testing using the Material Testing System (MTS™ System Corp, Eden Prairie, MN) machine platform. Following a midline infraumbilical laparotomy, bowel packing and retraction, and adequate exposure of the uterine horns and adjacent pelvic sidewall, a salpingostomy is made using electrocautery 1cm caudal the uterus-fallopian tube junction. A 7cm 8fr. latex urinary catheter, reinforced with a coaxial internal semi-rigid 5fr. polypropylene catheter, is inserted until it lies entirely within the lumen of the uterus. A 10cm segment of 6.35mm ID latex rubber drain tubing is secured to the dorsal aspect of the broad ligament medial the uterine horn; this is placed to prevent sidewall-broad ligament adhesion avoiding interference with the sidewall-uterus adhesion. The uterus and latex rubber drain are attached to the sidewall of the pelvis. The peritoneum lateral to the attached uterus is coagulated along the full length of the catheter insert at a setting of 6/10 (17W output) using a shielded electrocautery tip; cauterized area corresponds to the uterine horn lie and is limited to the peritoneum only. This injury is mirrored on the cannulated uterus to desiccate the superficial layer, and repeated on the contralateral side. Upon completion of the injury, antibiotic rinse is administered, excess fluid is removed via suction, packing and retractors are removed and abdomen is closed. Following a 2-week survival, a midline laparotomy is again performed; the surgeon visual assesses the pelvic uterine horn adhesion. Euthanasia is achieved and the entire complex of cannulated uterine horn, sutures, adherent pelvic sidewall and muscle is removed en bloc. The sample is marked cranial and caudally for reference; muscle, broad ligament, and latex rubber drain are cautiously dissected away. The remaining uterine horn and pelvic sidewall is cut at 1.5cm and 4.5cm from the cranial suture. The catheters are replaced by a 0.64cm OD by 8cm stainless steel rod. The length of uterus being tested is measured and recorded. Rubber O-rings, 0.95cm OD and 1.28cm OD, are placed to prevent lateral movement and stabilize the specimen during testing. The peritoneal sidewall is tightly secured within a jig clamp and the complex is loaded on the MTS… Advisors/Committee Members: Newby, Dr. Bi-min (Advisor).

Subjects/Keywords: Engineering; Ketorlac Tromethamine; Adhesions; Swine; PLGA; microspheres

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Cheung, M. E. (2010). A Swine Model for the Quantification of Pelvic Adhesions and the Encapsulation of Ketorolac Tromethamine for the Prevention of Adhesion Formation. (Masters Thesis). University of Akron. Retrieved from http://rave.ohiolink.edu/etdc/view?acc_num=akron1280274930

Chicago Manual of Style (16^th Edition):

Cheung, Maureen Elizabeth. “A Swine Model for the Quantification of Pelvic Adhesions and the Encapsulation of Ketorolac Tromethamine for the Prevention of Adhesion Formation.” 2010. Masters Thesis, University of Akron. Accessed October 15, 2019. http://rave.ohiolink.edu/etdc/view?acc_num=akron1280274930.

MLA Handbook (7^th Edition):

Cheung, Maureen Elizabeth. “A Swine Model for the Quantification of Pelvic Adhesions and the Encapsulation of Ketorolac Tromethamine for the Prevention of Adhesion Formation.” 2010. Web. 15 Oct 2019.

Vancouver:

Cheung ME. A Swine Model for the Quantification of Pelvic Adhesions and the Encapsulation of Ketorolac Tromethamine for the Prevention of Adhesion Formation. [Internet] [Masters thesis]. University of Akron; 2010. [cited 2019 Oct 15]. Available from: http://rave.ohiolink.edu/etdc/view?acc_num=akron1280274930.

Council of Science Editors:

Cheung ME. A Swine Model for the Quantification of Pelvic Adhesions and the Encapsulation of Ketorolac Tromethamine for the Prevention of Adhesion Formation. [Masters Thesis]. University of Akron; 2010. Available from: http://rave.ohiolink.edu/etdc/view?acc_num=akron1280274930

12. Yang, Jessica Pei-Wen. ELECTROSPUN PLGA MICROFIBERS FOR LOCALIZED DELIVERY OF SMALL MOLECULES TO INDUCE BROWN ADIPOGENESIS.

Degree: 2014, Johns Hopkins University

URL: http://jhir.library.jhu.edu/handle/1774.2/37102

► Obesity and its related disorders have been on the rise in the last few decades. Current prescribed treatments range from diet and physical activity for… (more)

Subjects/Keywords: Brown fat; localized delivery; rosiglitazone; PLGA

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APA (6^th Edition):

Yang, J. P. (2014). ELECTROSPUN PLGA MICROFIBERS FOR LOCALIZED DELIVERY OF SMALL MOLECULES TO INDUCE BROWN ADIPOGENESIS. (Thesis). Johns Hopkins University. Retrieved from http://jhir.library.jhu.edu/handle/1774.2/37102

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Yang, Jessica Pei-Wen. “ELECTROSPUN PLGA MICROFIBERS FOR LOCALIZED DELIVERY OF SMALL MOLECULES TO INDUCE BROWN ADIPOGENESIS.” 2014. Thesis, Johns Hopkins University. Accessed October 15, 2019. http://jhir.library.jhu.edu/handle/1774.2/37102.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Yang, Jessica Pei-Wen. “ELECTROSPUN PLGA MICROFIBERS FOR LOCALIZED DELIVERY OF SMALL MOLECULES TO INDUCE BROWN ADIPOGENESIS.” 2014. Web. 15 Oct 2019.

Vancouver:

Yang JP. ELECTROSPUN PLGA MICROFIBERS FOR LOCALIZED DELIVERY OF SMALL MOLECULES TO INDUCE BROWN ADIPOGENESIS. [Internet] [Thesis]. Johns Hopkins University; 2014. [cited 2019 Oct 15]. Available from: http://jhir.library.jhu.edu/handle/1774.2/37102.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Yang JP. ELECTROSPUN PLGA MICROFIBERS FOR LOCALIZED DELIVERY OF SMALL MOLECULES TO INDUCE BROWN ADIPOGENESIS. [Thesis]. Johns Hopkins University; 2014. Available from: http://jhir.library.jhu.edu/handle/1774.2/37102

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Penn State University

13. Fattahi, Pouria. Characterization Of The Size, Shape, And Drug Encapsulation Efficiency Of Plga Microcapsules Produced Via Electrojetting For Anticancer Agent Delivery.

Degree: MS, Bioengineering, 2013, Penn State University

URL: https://etda.libraries.psu.edu/catalog/19927

► Despite significant progress in the development of new chemotherapeutic agents and drug delivery methods for brain tumors, malignant gliomas (high grade brain tumor) remains deadly… (more)

▼ Despite significant progress in the development of new chemotherapeutic agents and drug delivery methods for brain tumors, malignant gliomas (high grade brain tumor) remains deadly with a median survival period of only about a year. The high dosage of chemotherapeutic agents required for penetration through the blood brain barrier during chemotherapy not only kills cancer cells but also damages healthy tissues and causes adverse side effects. Hence, a major unmet challenge in the treatment of malignant gliomas is the development of effective and targeted local delivery of chemotherapeutic agents at the cellular level. Drug-loaded biodegradable microcapsules with high drug encapsulation efficiency and controlled shape and size are attractive candidates for a more precise control of anticancer agent delivery at the tumor sites. Here, I report the results of a systematic study of the size, shape, and drug release profiles of Poly(lactic-co glycolic) (PLGA) microcapsules produced and loaded with the anticancer agent 1,3-bis(2-chloroethyl)-1-nitrosourea (BCNU) using an electrojetting technique. I report production of BCNU-loaded PLGA microcapsules in the form of flattened microspheres, microspheres, and microfibers with significantly (1) higher drug encapsulation efficiency, (2) more tunable drug loading capacity, and (3) narrower size distribution than those generated using other encapsulation methods. To prepare BCNU-PLGA solutions with PLGA concentrations ranging from 1 to 10 wt%, BCNU/PLGA mixtures (5 to 25 wt% with respect to PLGA) dissolved in chloroform were electrojetted on gold substrates. I quantified the shape and size distribution of BCNU-loaded PLGA microcapsules as a function of the polymer concentration and flow rate used during electrojetting, and characterized drug release profiles for microcapsules of three different morphologies: flattened microspheres, microspheres, and microfibers. Flattened microspheres were produced from 1 and 2 wt% PLGA solutions, while microspheres and microfibers were formed as the PLGA concentration increased to 3-5 wt% and 10% wt%, respectively. I investigated the effect of flow rate (i.e. 0.25, 0.5 and 1 mLh-1) on the size and monodispersity of BCNU-loaded PLGA microcapsules. Microcapsules of 1 wt% PLGA, 4 wt% PLGA, and 10 wt% PLGA formed at a flow rate of Q= 0.25 mLh-1 had narrower size distributions (CV= 4.6%, CV= 4.5%, and CV= 4.8%, respectively). A commonly accepted definition of monodispersity is CV < 5%. I also measured drug release profiles and developed mathematical models to estimate the effective diffusion coefficient of BCNU in different PLGA microcapsules. The BCNU release profiles for all three microcapsule morphologies were found to be in good agreement with model predictions for drug release as a result of drug diffusion and degradation of PLGA microcapsules.

Subjects/Keywords: BCNU; Drug delivery; PLGA; Mathematical modeling

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Fattahi, P. (2013). Characterization Of The Size, Shape, And Drug Encapsulation Efficiency Of Plga Microcapsules Produced Via Electrojetting For Anticancer Agent Delivery. (Masters Thesis). Penn State University. Retrieved from https://etda.libraries.psu.edu/catalog/19927

Chicago Manual of Style (16^th Edition):

Fattahi, Pouria. “Characterization Of The Size, Shape, And Drug Encapsulation Efficiency Of Plga Microcapsules Produced Via Electrojetting For Anticancer Agent Delivery.” 2013. Masters Thesis, Penn State University. Accessed October 15, 2019. https://etda.libraries.psu.edu/catalog/19927.

MLA Handbook (7^th Edition):

Fattahi, Pouria. “Characterization Of The Size, Shape, And Drug Encapsulation Efficiency Of Plga Microcapsules Produced Via Electrojetting For Anticancer Agent Delivery.” 2013. Web. 15 Oct 2019.

Vancouver:

Fattahi P. Characterization Of The Size, Shape, And Drug Encapsulation Efficiency Of Plga Microcapsules Produced Via Electrojetting For Anticancer Agent Delivery. [Internet] [Masters thesis]. Penn State University; 2013. [cited 2019 Oct 15]. Available from: https://etda.libraries.psu.edu/catalog/19927.

Council of Science Editors:

Fattahi P. Characterization Of The Size, Shape, And Drug Encapsulation Efficiency Of Plga Microcapsules Produced Via Electrojetting For Anticancer Agent Delivery. [Masters Thesis]. Penn State University; 2013. Available from: https://etda.libraries.psu.edu/catalog/19927

University of California – Irvine

14. Rapier, Crystal Eunique. DROPLET BASED MICROFLUIDICS FOR THE DEVELOPMENT OF A NOVEL BIOABSORBABLE IMMUNOMODULATORY MATERIAL WITH APPLICATIONS IN THE IMPLANTABLE MEDICAL DEVICE FIELD.

Degree: Biomedical Engineering, 2016, University of California – Irvine

URL: http://www.escholarship.org/uc/item/30d2s9kn

► Inflammation and the foreign body response (FBR) pose a major problem to the successful function of essentially “foreign” implanted medical devices such as vascular stents,… (more)

Subjects/Keywords: Biomedical engineering; Immunology; Immunomodulatory; Microfluidics; PLGA

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APA (6^th Edition):

Rapier, C. E. (2016). DROPLET BASED MICROFLUIDICS FOR THE DEVELOPMENT OF A NOVEL BIOABSORBABLE IMMUNOMODULATORY MATERIAL WITH APPLICATIONS IN THE IMPLANTABLE MEDICAL DEVICE FIELD. (Thesis). University of California – Irvine. Retrieved from http://www.escholarship.org/uc/item/30d2s9kn

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Rapier, Crystal Eunique. “DROPLET BASED MICROFLUIDICS FOR THE DEVELOPMENT OF A NOVEL BIOABSORBABLE IMMUNOMODULATORY MATERIAL WITH APPLICATIONS IN THE IMPLANTABLE MEDICAL DEVICE FIELD.” 2016. Thesis, University of California – Irvine. Accessed October 15, 2019. http://www.escholarship.org/uc/item/30d2s9kn.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Rapier, Crystal Eunique. “DROPLET BASED MICROFLUIDICS FOR THE DEVELOPMENT OF A NOVEL BIOABSORBABLE IMMUNOMODULATORY MATERIAL WITH APPLICATIONS IN THE IMPLANTABLE MEDICAL DEVICE FIELD.” 2016. Web. 15 Oct 2019.

Vancouver:

Rapier CE. DROPLET BASED MICROFLUIDICS FOR THE DEVELOPMENT OF A NOVEL BIOABSORBABLE IMMUNOMODULATORY MATERIAL WITH APPLICATIONS IN THE IMPLANTABLE MEDICAL DEVICE FIELD. [Internet] [Thesis]. University of California – Irvine; 2016. [cited 2019 Oct 15]. Available from: http://www.escholarship.org/uc/item/30d2s9kn.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Rapier CE. DROPLET BASED MICROFLUIDICS FOR THE DEVELOPMENT OF A NOVEL BIOABSORBABLE IMMUNOMODULATORY MATERIAL WITH APPLICATIONS IN THE IMPLANTABLE MEDICAL DEVICE FIELD. [Thesis]. University of California – Irvine; 2016. Available from: http://www.escholarship.org/uc/item/30d2s9kn

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Sydney

15. Ashhurt, Anneliese. Pulmonary vaccines and immune responses to control tuberculosis .

Degree: 2017, University of Sydney

URL: http://hdl.handle.net/2123/17207

► Tuberculosis remains a staggering burden on global health, despite considerable research efforts toward preventative strategies. Subunit vaccines offer potential as safe generators of protective immunity,… (more)

▼ Tuberculosis remains a staggering burden on global health, despite considerable research efforts toward preventative strategies. Subunit vaccines offer potential as safe generators of protective immunity, and there is growing interest in exploring delivery by the pulmonary route to enhance immunogenicity. In this study, novel subunit vaccines were designed and produced utilising a variety of adjuvants and protein antigens from Mycobacterium tuberculosis (Mtb), with a focus on formulation for pulmonary delivery. The immunogenicity and protective efficacy of these vaccines was assessed in a murine model of Mtb infection. First, the novel TLR2-ligand adjuvant, Lipokel®, was non-covalently conjugated to proteins from Mtb and formulated as dry powders for inhalation, delivering antigen into the lungs in a self-adjuvanting context. Second, immunodominant peptide epitopes from Mtb were covalently conjugated to Pam2Cys to provide stable lipopeptide vaccines. Third, strategies for covalent conjugation of full length recombinant proteins from Mtb to Pam2Cys are shown. Finally, the novel polysaccharide adjuvant AdvaxTM was tested in combination with fusion proteins as a pulmonary vaccine and this resulted in highly significant protection from Mtb challenge. A different approach used biodegradable poly(lactic-co-glycolic acid) particles as a carrier for MPT83 and adjuvants, formulated for pulmonary delivery. The protective efficacy of these particulate vaccines against Mtb was compared to the effect of DDA-liposome based vaccine formulations. Chemokine receptors play an important role in the recruitment of T-cells to the lungs following immunisation or infection. Therefore the role of CXCR6 was examined in early and chronic Mtb infection, and this was compared to the CD4+ T-cell responses induced by a recombinant influenza A virus vaccine. Paradoxically, CXCR6 deficiency increased protection against these pathogens and resulted in improved control of Mtb infection. These studies emphasise the critical nature of adjuvant choice and administration route on the efficacy of TB vaccines, and the novel effective vaccines developed in this study represent a new approach to prevent TB.

Subjects/Keywords: Tuberculosis; Pulmonary vaccines; Pam2Cys; Advax; PLGA; CXCR6

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Ashhurt, A. (2017). Pulmonary vaccines and immune responses to control tuberculosis . (Thesis). University of Sydney. Retrieved from http://hdl.handle.net/2123/17207

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Ashhurt, Anneliese. “Pulmonary vaccines and immune responses to control tuberculosis .” 2017. Thesis, University of Sydney. Accessed October 15, 2019. http://hdl.handle.net/2123/17207.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Ashhurt, Anneliese. “Pulmonary vaccines and immune responses to control tuberculosis .” 2017. Web. 15 Oct 2019.

Vancouver:

Ashhurt A. Pulmonary vaccines and immune responses to control tuberculosis . [Internet] [Thesis]. University of Sydney; 2017. [cited 2019 Oct 15]. Available from: http://hdl.handle.net/2123/17207.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Ashhurt A. Pulmonary vaccines and immune responses to control tuberculosis . [Thesis]. University of Sydney; 2017. Available from: http://hdl.handle.net/2123/17207

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Manchester

16. Fraylich, Michael. Colloidal Delivery Systems.

Degree: 2010, University of Manchester

URL: http://www.manchester.ac.uk/escholar/uk-ac-man-scw:89507

► In this project we aim to produce a thermally triggered PLGA particulate gel, which is injectable and biocompatible. This will act as a scaffold for… (more)

Subjects/Keywords: Colloid; PEGMA; PPGMA; PLGA; Thermal; Temperature; Gelation

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Fraylich, M. (2010). Colloidal Delivery Systems. (Doctoral Dissertation). University of Manchester. Retrieved from http://www.manchester.ac.uk/escholar/uk-ac-man-scw:89507

Chicago Manual of Style (16^th Edition):

Fraylich, Michael. “Colloidal Delivery Systems.” 2010. Doctoral Dissertation, University of Manchester. Accessed October 15, 2019. http://www.manchester.ac.uk/escholar/uk-ac-man-scw:89507.

MLA Handbook (7^th Edition):

Fraylich, Michael. “Colloidal Delivery Systems.” 2010. Web. 15 Oct 2019.

Vancouver:

Fraylich M. Colloidal Delivery Systems. [Internet] [Doctoral dissertation]. University of Manchester; 2010. [cited 2019 Oct 15]. Available from: http://www.manchester.ac.uk/escholar/uk-ac-man-scw:89507.

Council of Science Editors:

Fraylich M. Colloidal Delivery Systems. [Doctoral Dissertation]. University of Manchester; 2010. Available from: http://www.manchester.ac.uk/escholar/uk-ac-man-scw:89507

Universidade Estadual de Campinas

17. Profirio, Daniel de Moraes, 1989-. Preparação de nanopartículas de PLGA contendo carboplatina e sua funcionalização com folato de quitosana .

Degree: 2018, Universidade Estadual de Campinas

URL: http://repositorio.unicamp.br/jspui/handle/REPOSIP/333377

► Resumo: Carboplatina é um fármaco análogo da cisplatina, não-específico, que modifica a estrutura do DNA e inibe sua replicação, causando morte celular. Apesar do potencial… (more)

▼ Resumo: Carboplatina é um fármaco análogo da cisplatina, não-específico, que modifica a estrutura do DNA e inibe sua replicação, causando morte celular. Apesar do potencial efeito antineoplásico, a carboplatina provoca efeitos adversos como mielossupressão, trombocitopenia e a baixa captação celular pelas células de câncer contribui para sua baixa eficiência terapêutica. Este trabalho teve como objetivo a encapsulação da carboplatina utilizando nanopartículas de PLGA como sistema carreador, e TPGS como surfactante. Nanopartículas de PLGA recobertas com folato de quitosana (QTS-FOL) também foram preparadas, sendo utilizado planejamento experimental para a otimização de ambos os sistemas de nanopartículas. Para as partículas não-modificadas, os resultados mostraram que o diâmetro médio e o PDI são dependentes do tempo, amplitude de sonicação e volume da fase aquosa, enquanto o potencial Zeta é constante. A formulação otimizada foi estável por um período de 60 dias quando armazenada a 10ºC em água deionizada, e a diálise apresentou melhores resultados como método de purificação. Para as partículas funcionalizadas, o diâmetro médio, PDI e potencial Zeta foram dependentes do tempo de agitação e da concentração da solução de folato de quitosana. As melhores condições para liofilização das partículas, sem mudanças nos valores de diâmetro médio e PDI, foram obtidas com 96 h e 30% m/V de trealose. Ambas as formulações apresentaram boa estabilidade em tampão PBS pH 7,4. A partir da técnica de fluorescência, o ensaio com o corante SYBR Green I sugeriu que a carboplatina pode ter mantido sua capacidade de se ligar ao DNA após a encapsulação. Difusão e intumescimento são fenômenos envolvidos no processo de liberação da carboplatina a partir das nanopartículas de PLGA; Abstract: Carboplatin is a cisplatin analogue and non-specific drug that modifies DNA structure and inhibits its replication, causing cell death. Despite the potential antineoplasic effect, carboplatin causes adverse effects as myelosuppression, thrombocytopenia and its lower cellular uptake by cancer cells contributes to its low therapeutic efficacy. The goal of this work was encapsulation of carboplatin using PLGA nanoparticles as carrier system, and TPGS as surfactant. Folic acid-conjugated chitosan-coated (FA-CS) PLGA nanoparticles were also prepared, using experimental design for optimization of both unmodified and surface modified nanoparticles. For PLGA nanoparticles, the results showed that mean particle size and PDI are dependents of time, amplitude of sonication and volume of aqueous solution, while Zeta potential is constant. The optimized formulation was stable over a period of 60 days when stored at 10ºC in deionized water, and dialysis showed better results as purification method. For surface modified nanoparticles, mean particle size, PDI and Zeta potential were dependent of stirring time and concentration of FA-CS solution. The best conditions for lyophilization of the particles, with no changes in mean particle size and PDI values, were obtained… Advisors/Committee Members: Pessine, Francisco Benedito Teixeira, 1948- (advisor), Ferreira, Marcia Miguel Castro (committee member), Corbi, Pedro Paulo (committee member), Silva, Denise de Oliveira (committee member), Silva, Andre Romero da (committee member).

Subjects/Keywords: Encapsulação; PLGA; Carboplatina; Ácido fólico; Planejamento experimental

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Profirio, Daniel de Moraes, 1. (2018). Preparação de nanopartículas de PLGA contendo carboplatina e sua funcionalização com folato de quitosana . (Thesis). Universidade Estadual de Campinas. Retrieved from http://repositorio.unicamp.br/jspui/handle/REPOSIP/333377

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Profirio, Daniel de Moraes, 1989-. “Preparação de nanopartículas de PLGA contendo carboplatina e sua funcionalização com folato de quitosana .” 2018. Thesis, Universidade Estadual de Campinas. Accessed October 15, 2019. http://repositorio.unicamp.br/jspui/handle/REPOSIP/333377.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Profirio, Daniel de Moraes, 1989-. “Preparação de nanopartículas de PLGA contendo carboplatina e sua funcionalização com folato de quitosana .” 2018. Web. 15 Oct 2019.

Vancouver:

Profirio, Daniel de Moraes 1. Preparação de nanopartículas de PLGA contendo carboplatina e sua funcionalização com folato de quitosana . [Internet] [Thesis]. Universidade Estadual de Campinas; 2018. [cited 2019 Oct 15]. Available from: http://repositorio.unicamp.br/jspui/handle/REPOSIP/333377.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Profirio, Daniel de Moraes 1. Preparação de nanopartículas de PLGA contendo carboplatina e sua funcionalização com folato de quitosana . [Thesis]. Universidade Estadual de Campinas; 2018. Available from: http://repositorio.unicamp.br/jspui/handle/REPOSIP/333377

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Brno University of Technology

18. Oborná, Jana. Řízené uvolňování léčiv z biodegradabilních hydrogelů.

Degree: 2018, Brno University of Technology

URL: http://hdl.handle.net/11012/84164

► Předkládaná dizertační práce je zaměřena na řízené uvolňování léčiv z biodegradabilních amfifilních hydrogelů na bázi kopolymeru hydrofobní kyseliny poly(mléčné) a poly(glykolové) a hydrofilního polyethylenglykolu (PLGA-PEG-PLGA,… (more)

▼ Předkládaná dizertační práce je zaměřena na řízené uvolňování léčiv z biodegradabilních amfifilních hydrogelů na bázi kopolymeru hydrofobní kyseliny poly(mléčné) a poly(glykolové) a hydrofilního polyethylenglykolu (PLGA-PEG-PLGA, ABA) a jeho modifikace anhydridem kyseliny itakonové (ITA). Výsledný ,-itakonyl(PLGA-PEG-PLGA) je označovaný v této práci jako ITA/PLGA-PEG-PLGA/ITA neboli ITA/ABA/ITA. Kyselina itakonová poskytuje reaktivní dvojné vazby a funkční karboxylovou skupinu na koncích řetězce PLGA-PEG-PLGA kopolymeru, čímž se modifikovaný kopolymer ITA/ABA/ITA stává méně hydrofobním a nabízí tak možnost tvořit nosič i pro léčivé látky hydrofilní povahy. Tyto funkční kopolymery jsou citlivé na teplo a změnou vnějšího prostředí (teploty) vykazují sol-gel fázový přechod díky vzniku micelární struktury gelu. Léčivé látky tak mohou být smíchány s kopolymerem, který je v nízko viskózní fázi (sol fáze) a následně tato směs může být injektována do těla pacienta na cílové místo, kde vytvoří gel při 37 °C. Tento hydrogel se stává zásobníkem léčivé látky, odkud se postupně substance uvolňuje. Predikce trendu uvolňování léčivé látky z hydrogelu je účinný nástroj pro zjištění frekvence podávání, zvýšení účinnosti léčiva a také pro zjištění nežádoucích účinků spojené s dávkováním. Jako hydrofilní modelové léčivo bylo vybráno analgetikum paracetamol. Zástupce hydrofobního modelového léčiva bylo použito sulfonamidové antibiotikum sulfathiazol. Přídání léčivé látky do roztoku kopolymeru mělo následně významný vliv na výslednou tuhost hydrogelu. Vliv na tuhost hydrogelu a následnou stabilitu systému mělo použití nanohydroxyapatitu i různých rozpouštědel resp. inkubačních médií. Řízené uvolňování léčiv probíhalo v simulovaných podmínkách lidského těla. Tato práce se zabývá ověřením Korsmeyer-Peppasova modelu vhodného pro popis uvolňování hydrofobního modelového léčiva sulfathiazolu z hydrogelu ABA i ITA/ABA/ITA. Naopak bylo prokázáno, že Korsmeyer-Peppasův model není vhodný pro popis uvolňování hydrofilního léčiva paracetamolu z ABA hydrogelů, proto byl navržen nový regresní model, vhodný jak pro pufrované simulované prostředí, tak i pro vodu. Tento model byl také ověřen za velmi těsný i pro popis uvolňování sulfathiazolu i paracetamolu z kompozitního materiálu připraveného z ABA hydrogelu a nanohydroxyapatitu.; This dissertation is focused on the controlled release of drugs from a biodegradable amphiphilic hydrogel based on hydrophobic poly(lactic acid), poly(glycolic acid) and hydrophilic poly(ethylene glycol) (PLGA-PEG-PLGA, ABA) and its modification with itaconic anhydride (ITA). The resulting ,-itaconyl(PLGA-PEG-PLGA) copolymer is referred to as ITA/PLGA-PEG-PLGA/ITA or ITA/ABA/ITA. Itaconic acid provides reactive double bonds and a functional carboxyl group at the ends of the PLGA-PEG-PLGA copolymer chain, thereby rendering the modified ITA/ABA/ITA copolymer less hydrophobic and offering the possibility of forming a carrier for hydrophilic drug substances. These functional copolymers are thermosensitive and change in the… Advisors/Committee Members: Vávrová, Milada (advisor).

Subjects/Keywords: hydrogel; PLGA-PEG-PLGA kopolymer; profil uvolňování léčiv; léčiva; Korsmeyer-Peppasův model; hydrogel; PLGA-PEG-PLGA copolymer; drug release profile; drugs; Korsmeyer-Peppas model

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Oborná, J. (2018). Řízené uvolňování léčiv z biodegradabilních hydrogelů. (Thesis). Brno University of Technology. Retrieved from http://hdl.handle.net/11012/84164

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Oborná, Jana. “Řízené uvolňování léčiv z biodegradabilních hydrogelů. ” 2018. Thesis, Brno University of Technology. Accessed October 15, 2019. http://hdl.handle.net/11012/84164.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Oborná, Jana. “Řízené uvolňování léčiv z biodegradabilních hydrogelů. ” 2018. Web. 15 Oct 2019.

Vancouver:

Oborná J. Řízené uvolňování léčiv z biodegradabilních hydrogelů. [Internet] [Thesis]. Brno University of Technology; 2018. [cited 2019 Oct 15]. Available from: http://hdl.handle.net/11012/84164.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Oborná J. Řízené uvolňování léčiv z biodegradabilních hydrogelů. [Thesis]. Brno University of Technology; 2018. Available from: http://hdl.handle.net/11012/84164

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Iowa State University

19. Chen, Yunqing. An implantable sustained-release chemotherapy delivery system for the treatment of breast cancer.

Degree: 2015, Iowa State University

URL: https://lib.dr.iastate.edu/etd/14784

► The influence of chitosan on the degradation characteristics of paclitaxel-loaded Poly (Lactic-co-Glycolic Acid) (PLGA) rod-shaped implants was investigated and modeled. The implant was designed for… (more)

Subjects/Keywords: Industrial Engineering; Chitosan; Cryomilling; PLGA; Engineering

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Chen, Y. (2015). An implantable sustained-release chemotherapy delivery system for the treatment of breast cancer. (Thesis). Iowa State University. Retrieved from https://lib.dr.iastate.edu/etd/14784

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Chen, Yunqing. “An implantable sustained-release chemotherapy delivery system for the treatment of breast cancer.” 2015. Thesis, Iowa State University. Accessed October 15, 2019. https://lib.dr.iastate.edu/etd/14784.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Chen, Yunqing. “An implantable sustained-release chemotherapy delivery system for the treatment of breast cancer.” 2015. Web. 15 Oct 2019.

Vancouver:

Chen Y. An implantable sustained-release chemotherapy delivery system for the treatment of breast cancer. [Internet] [Thesis]. Iowa State University; 2015. [cited 2019 Oct 15]. Available from: https://lib.dr.iastate.edu/etd/14784.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Chen Y. An implantable sustained-release chemotherapy delivery system for the treatment of breast cancer. [Thesis]. Iowa State University; 2015. Available from: https://lib.dr.iastate.edu/etd/14784

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Universidade Estadual de Campinas

20. Calderini, Adriana. Preparação e caracterização de nano-esferas de PLGA contendo 5-fluorouracil e estudo do acoplamento de quitosana e ácido fólico em sua superfície .

Degree: 2011, Universidade Estadual de Campinas

URL: http://repositorio.unicamp.br/jspui/handle/REPOSIP/250353

► Resumo: 5-Fluorouracil (5-FU) e um dos fármacos mais úteis no tratamento de tumores sólidos em adultos, especificamente carcinomas do trato gastrointestinal (estômago, cólon e reto)… (more)

▼ Resumo: 5-Fluorouracil (5-FU) e um dos fármacos mais úteis no tratamento de tumores sólidos em adultos, especificamente carcinomas do trato gastrointestinal (estômago, cólon e reto) e da mama. Em resumo, seu maior efeito bioquímico e a inibição da síntese do DNA, pois a concentração que inibe sua síntese pode ainda permitir a sintese do RNA. Causa severos efeitos adversos como mielo supressão, mucosite, dermatite, diarréia e toxicidade cardíaca. Em razão disto, têm sido realizadas várias tentativas para encapsular 5-FU a fim de reduzir os efeitos adversos que ele provoca. O objetivo deste projeto foi encapsular este anti-neoplásico utilizando nano-esferas de ácido poli(lático-co-glicólico), PLGA, como sistema carreador, acoplando à sua superfície quitosana e folato de quitosana para melhor endereçamento aos locais de ação, bioadesividade e menor toxicidade. A encapsulação de 5-FU em nano-esferas de PLGA foi aperfeiçoada através de planejamento experimental e por análise quimiométrica. Muitos fatores foram estudados: métodos de preparação, temperatura, quantidade inicial de 5-FU e pH. Na caracterização destes sistemas foram utilizadas diversas técnicas: espalhamento dinâmico de luz, determinação do potencial Zeta, calorimetria diferencial de varredura, análise termogravimétrica, difratometria de raios-X e microscopia eletrônica de varredura. Além disso, foram realizados ensaios de perfil de liberação, de estabilidade coloidal e estudo do comportamento desses sistemas em relação às células in vitro. O planejamento experimental permitiu obter nanoparticulas com capacidade de carregamento em torno de 11% e eficiência de encapsulação de 32%. O acoplamento de quitosana e folato de quitosana permitiu retardar a liberação do fármaco em solução. Para armazenagem destas partículas, observou-se que elas foram menos degradadas quando estão liofilizadas e mantidas a 4 °C. A melhor concentração de sacarose para liofilizá-las, sem que ocorra aumento de tamanho e polidispersão, foi 250 mmol/L. Os testes in vitro comprovaram a eficácia destas formulações; Abstract: 5-fluorouracil (5-fluoro-1H-pyrimidine-2,4-dione) is one of the most used drug to treat solid tumors in adults, specifically gastrointestinal (stomach and colorectal) and breast carcinomas. In summary, the major biochemical effect of 5-FU is inhibition of DNA synthesis, since concentrations which inhibit this synthesis may still permit RNA synthesis. It causes severe adverse effects as myelosuppression, mucositis, dermatitis, diarrhea and cardiac toxicity. Its encapsulation in nanoparticles can reduce these adverse effects, prolong its release and the drug can be placed directly on its site of action. The goal of this work was to encapsulate this anti-neoplasic drug using poly (lactic-co-glycolic acid) PLGA nanospheres as carrier system, attaching on their surface chitosan and folate-chitosan to attain an enhanced targeting, bioadesivity and less toxicity. The encapsulation of 5-FU in PLGA nanospheres was improved through an experimental design and chemometry. Many factors… Advisors/Committee Members: Pessine, Francisco Benedito Teixeira, 1948- (advisor), Silva, Andre Romero da (committee member), Fraceto, Leonardo Fernandes (committee member), Jorge, Renato Atilio (committee member), Volpe, Pedro Luiz Onófrio (committee member).

Subjects/Keywords: 5-Fluorouracil; PLGA; Quitosana; Vitamina M

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Calderini, A. (2011). Preparação e caracterização de nano-esferas de PLGA contendo 5-fluorouracil e estudo do acoplamento de quitosana e ácido fólico em sua superfície . (Thesis). Universidade Estadual de Campinas. Retrieved from http://repositorio.unicamp.br/jspui/handle/REPOSIP/250353

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Calderini, Adriana. “Preparação e caracterização de nano-esferas de PLGA contendo 5-fluorouracil e estudo do acoplamento de quitosana e ácido fólico em sua superfície .” 2011. Thesis, Universidade Estadual de Campinas. Accessed October 15, 2019. http://repositorio.unicamp.br/jspui/handle/REPOSIP/250353.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Calderini, Adriana. “Preparação e caracterização de nano-esferas de PLGA contendo 5-fluorouracil e estudo do acoplamento de quitosana e ácido fólico em sua superfície .” 2011. Web. 15 Oct 2019.

Vancouver:

Calderini A. Preparação e caracterização de nano-esferas de PLGA contendo 5-fluorouracil e estudo do acoplamento de quitosana e ácido fólico em sua superfície . [Internet] [Thesis]. Universidade Estadual de Campinas; 2011. [cited 2019 Oct 15]. Available from: http://repositorio.unicamp.br/jspui/handle/REPOSIP/250353.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Calderini A. Preparação e caracterização de nano-esferas de PLGA contendo 5-fluorouracil e estudo do acoplamento de quitosana e ácido fólico em sua superfície . [Thesis]. Universidade Estadual de Campinas; 2011. Available from: http://repositorio.unicamp.br/jspui/handle/REPOSIP/250353

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Rochester

21. Tseng, Kuang-Ching Chris (1981 - ); Elfar, John C. The potential therapeutic approaches to improve peripheral nerve functional recovery from injury.

Degree: PhD, 2015, University of Rochester

URL: http://hdl.handle.net/1802/29718

► Traumatic peripheral nerve crush injuries contribute a great deal of morbidity in patients and little improvement has been made in the treatment of these injuries… (more)

▼ Traumatic peripheral nerve crush injuries contribute a great deal of morbidity in patients and little improvement has been made in the treatment of these injuries in many years. Diagnosis and treatment of traumatic peripheral nerve damage suffer from a lack of means of accurately diagnosing the reasons for loss of nerve function and a lack of means of enhancing the time course of repair in injuries in which nerve function is lost for reasons other than axonal transection. </br> In the current work, we demonstrate that administration of erythropoietin (EPO) shortens the time course of recovery after experimental crush injury to the mouse sciatic nerve. We found that systemic EPO administration maintained more myelinated axons at the site of injury in vivo following sciatic nerve crush. In vitro, EPO treatment promotes myelin formation and protects myelin from the effects of nitric oxide exposure in co-cultures of Schwann cells and dorsal root ganglion (DRG) neurons. These results suggested local EPO application might also be effective in promoting functional recovery, which would avoid potential systemic side effects of systemic EPO administration. We therefore developed a novel local treatment for peripheral nerve injury of EPO delivered in a fibrin glue matrix at the site of injury. Local delivery was as effective as systemic EPO administration, and thus may provide a potentially useful approach to enhancing recovery in peripheral nerve injuries. </br> We also demonstrate that a single administration of 4-aminopyridine (4-AP), which enables impulse conduction in demyelinated axons, transiently restores function and allows identification of injuries in which axons are intact but not functional. We further demonstrate that repeated 4-AP administration markedly improves the time course of recovery from crush injuries in the peripheral nerve as determined by behavioral and electophysiological analyses. Equal or greater efficacy is provided by localized delivery of slow-release formulations of 4-AP at the lesion site. In addition, we provide biochemical and ultrastructural evidence to show that repeated systemic 4-AP delivery and localized slow-release delivery enhance remyelination after injury. Thus, we provide a single therapeutic approach that addresses critical issues in diagnosing peripheral nerve injury and enhancing repair, and provides the first pharmacological approach to enhancing myelin repair. As 4-AP is already used in the clinic for treatment of multiple sclerosis, our approaches are highly suitable for rapid translation from the laboratory to clinical analysis.

Subjects/Keywords: 4-Aminopyridine; Erythropoietin; PLGA; Peripheral nerve injury

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Tseng, Kuang-Ching Chris (1981 - ); Elfar, J. C. (2015). The potential therapeutic approaches to improve peripheral nerve functional recovery from injury. (Doctoral Dissertation). University of Rochester. Retrieved from http://hdl.handle.net/1802/29718

Chicago Manual of Style (16^th Edition):

Tseng, Kuang-Ching Chris (1981 - ); Elfar, John C. “The potential therapeutic approaches to improve peripheral nerve functional recovery from injury.” 2015. Doctoral Dissertation, University of Rochester. Accessed October 15, 2019. http://hdl.handle.net/1802/29718.

MLA Handbook (7^th Edition):

Tseng, Kuang-Ching Chris (1981 - ); Elfar, John C. “The potential therapeutic approaches to improve peripheral nerve functional recovery from injury.” 2015. Web. 15 Oct 2019.

Vancouver:

Tseng, Kuang-Ching Chris (1981 - ); Elfar JC. The potential therapeutic approaches to improve peripheral nerve functional recovery from injury. [Internet] [Doctoral dissertation]. University of Rochester; 2015. [cited 2019 Oct 15]. Available from: http://hdl.handle.net/1802/29718.

Council of Science Editors:

Tseng, Kuang-Ching Chris (1981 - ); Elfar JC. The potential therapeutic approaches to improve peripheral nerve functional recovery from injury. [Doctoral Dissertation]. University of Rochester; 2015. Available from: http://hdl.handle.net/1802/29718

22. Melo, Carina Guimarães de Souza. Enxerto sintético e biológico, com e sem preenchimento de veia jugular externa, no reparo de nervo periférico em ratos.

Degree: Mestrado, Estomatologia e Biologia Oral, 2011, University of São Paulo

URL: http://www.teses.usp.br/teses/disponiveis/25/25149/tde-24082011-103123/ ;

► Apesar do desenvolvimento da tecnologia envolvendo o campo da regeneração em nervos periféricos, ainda não existe uma técnica para recuperação do tecido nervoso que apresente… (more)

▼ Apesar do desenvolvimento da tecnologia envolvendo o campo da regeneração em nervos periféricos, ainda não existe uma técnica para recuperação do tecido nervoso que apresente resultados totalmente satisfatórios, fato que desperta o interesse de vários pesquisadores em todo mundo e representa um desafio para os cirurgiões que realizam procedimentos reconstrutivos e estéticos. Embora o enxerto autólogo de nervo seja a melhor alternativa para a recuperação de nervos periféricos lesados, as técnicas que envolvem o reparo tubular têm alcançado excelentes resultados através da utilização de materiais sintéticos e biológicos, sob a forma de tubos, no reparo de extensos segmentos nervosos. Hoje, através dos avanços da engenharia tecidual, novos materiais estão em desenvolvimento, com o objetivo de aliar características microscópicas às técnicas cirúrgicas existentes. O colágeno, que é sabidamente um elemento promotor da proliferação celular e do reparo tecidual, tem sido amplamente utilizado em estudos de regeneração nervosa. Da mesma maneira, o ácido poli-láctico-poli-glicólico (PLGA), um copolímero sintético, tem apresentado algumas características favoráveis ao processo de regeneração, como biocompatibilidade e propriedades mecânicas adequadas. Com o propósito de facilitar a regeneração nervosa quando ocorre perda tecidual, uma técnica já difundida pode ser destacada: o enxerto de veia invertida, em que a veia jugular externa é utilizada ao avesso, funcionando como um tubo, criando um microambiente para a regeneração nervosa, através da exposição de elementos fundamentais da camada mais externa do vaso (túnica adventícia). Neste trabalho, agregamos como diferencial a utilização de dois tipos de membranas, especialmente desenvolvidas para fins odontológicos, que visam neoformação óssea, em um estudo que visa à regeneração de um nervo periférico misto, o nervo isquiático. As membranas de colágeno e PLGA foram colocadas na região do enxerto, sob a forma de tubo, com o objetivo de recuperar um segmento de 10 mm do nervo referido. E, por fim, aliamos a utilização de um segmento de 5 mm da veia jugular externa como tecido de preenchimento dos tubos feitos com as membranas, na tentativa de verificar a eficácia das moléculas biológicas presentes neste vaso, porém agora sendo utilizadas diretamente dentro do enxerto. Após um período de 6 semanas, o grupo que apresentou o melhor resultado foi aquele em que foi utilizada a membrana de colágeno, na forma de tubo, preenchida com a veia jugular externa. Nesse grupo, o diâmetro médio das fibras mielínicas apresentou um valor médio de 5,8 µm e espessura média da bainha de mielina de 1,65 µm. Para o período de 12 semanas, entre os grupos analisados, o maior valor médio encontrado para o diâmetro foi de 5,64 µm e 1,31 µm para a espessura, sendo que este resultado foi apresentado pelo grupo em que se utilizou a membrana de PLGA sem preenchimento com a veia jugular. Embora os valores não sejam muito inferiores, nos dois períodos, ficaram abaixo dos valores encontrados nos grupos… Advisors/Committee Members: Rodrigues, Antonio de Castro.

Subjects/Keywords: Colágeno; Collagen; Membranas; Membranes; Nervo periférico; Peripheral nerve; PLGA; PLGA; Regeneração; Regeneration; Tubulização; Tubulization

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Melo, C. G. d. S. (2011). Enxerto sintético e biológico, com e sem preenchimento de veia jugular externa, no reparo de nervo periférico em ratos. (Masters Thesis). University of São Paulo. Retrieved from http://www.teses.usp.br/teses/disponiveis/25/25149/tde-24082011-103123/ ;

Chicago Manual of Style (16^th Edition):

Melo, Carina Guimarães de Souza. “Enxerto sintético e biológico, com e sem preenchimento de veia jugular externa, no reparo de nervo periférico em ratos.” 2011. Masters Thesis, University of São Paulo. Accessed October 15, 2019. http://www.teses.usp.br/teses/disponiveis/25/25149/tde-24082011-103123/ ;.

MLA Handbook (7^th Edition):

Melo, Carina Guimarães de Souza. “Enxerto sintético e biológico, com e sem preenchimento de veia jugular externa, no reparo de nervo periférico em ratos.” 2011. Web. 15 Oct 2019.

Vancouver:

Melo CGdS. Enxerto sintético e biológico, com e sem preenchimento de veia jugular externa, no reparo de nervo periférico em ratos. [Internet] [Masters thesis]. University of São Paulo; 2011. [cited 2019 Oct 15]. Available from: http://www.teses.usp.br/teses/disponiveis/25/25149/tde-24082011-103123/ ;.

Council of Science Editors:

Melo CGdS. Enxerto sintético e biológico, com e sem preenchimento de veia jugular externa, no reparo de nervo periférico em ratos. [Masters Thesis]. University of São Paulo; 2011. Available from: http://www.teses.usp.br/teses/disponiveis/25/25149/tde-24082011-103123/ ;

23. Sicchieri, Luciana Gonçalves. Engenharia de tecido ósseo: avaliações in vitro e in vivo do biomaterial híbrido ácido poli-láctico-co-glicólico/fosfato de cálcio e células osteoblásticas derivadas de células-tronco.

Degree: Mestrado, Cirurgia Buco-Maxilo-Facial, 2010, University of São Paulo

URL: http://www.teses.usp.br/teses/disponiveis/58/58136/tde-17092010-084425/ ;

►

Tem sido sugerido que um adequado reparo ósseo pode ser obtido por biomateriais híbridos, produzidos pela combinação de células e materiais substitutos ósseos macroporosos. O… (more)

▼

Tem sido sugerido que um adequado reparo ósseo pode ser obtido por biomateriais híbridos, produzidos pela combinação de células e materiais substitutos ósseos macroporosos. O objetivo geral do presente estudo foi avaliar a aplicação do biomaterial híbrido formado pelo arcabouço de PLGA/CaP e células-tronco mesenquimais e osteoblastos derivados de medula óssea na engenharia de tecido ósseo. Para verificar qual o tamanho de poro deste arcabouço é mais adequado para este fim, foram realizados experimentos in vitro, que avaliaram a proliferação celular, atividade de fosfatase alcalina (ALP) e expressão quantitativa de genes marcadores do fenótipo osteoblástico em células cultivadas sobre os arcabouços; e in vivo, que avaliaram a formação óssea após implantação do arcabouço em defeitos ósseos críticos de calvária de ratos. Também foi avaliado o efeito do tamanho dos poros sobre o carreamento celular através da força centrifuga. Para avaliar o efeito do soro fetal bovino, utilizado na suplementação do meio de cultura celular, na resposta tecidual in vivo, arcabouços expostos ao soro foram implantados em defeitos ósseos críticos de calvária de ratos. O efeito da retirada do soro fetal bovino do meio de cultura sobre osteoblastos foi analisado através da proliferação celular, atividade de ALP, conteúdo de proteína total e mineralização. Para avaliar o efeito do estágio de diferenciação celular sobre a formação óssea, células em diferentes estágios de diferenciação osteoblástica associadas ao arcabouço de PLGA/CaP foram implantadas de forma autóloga em defeitos ósseos críticos de calvária de ratos. Arcabouços com tamanhos de poros de aproximadamente 1000 µm promovem maior diferenciação osteoblástica e melhor carreamento celular, enquanto arcabouços com poros de aproximadamente 500 µm promovem maior formação óssea e vascular in vivo. O soro fetal bovino influenciou negativamente a resposta tecidual e a formação óssea. A retirada do soro fetal bovino do meio de cultura reduziu a proliferação celular e a atividade de ALP sem afetar o conteúdo de proteína total e a formação de matriz mineralizada. Células-tronco mesenquimais indiferenciadas e em fase inicial de diferenciação osteoblástica (7 dias) promoveram maior formação óssea, portanto permitiriam a obtenção de um biomaterial híbrido com maior potencial osteogênico.

It has been suggested that an adequate bone repair can be obtained by hybrid biomaterials, produced by combining osteoprogenitor cells and macroporous bone substitutes. The aim of this study was to evaluate the application of hybrid biomaterial formed by PLGA/CaP scaffold and bone marrow-derived mesenchymal stem cells and osteoblasts in bone tissue engineering. The effect of scaffold pore size was evaluated in vitro assessing cell growth, alkaline phosphatase (ALP) activity, and quantitative gene expression of osteoblastic phenotype markers in osteoblasts cultured on scaffolds; and in vivo assesseing bone formation after implantation of scaffolds in critical size rat calvaria defects. It was also evaluated…

Advisors/Committee Members: Rosa, Adalberto Luiz.

Subjects/Keywords: bone engineering; células osteoprogenitoras; engenharia óssea; osteogênese; osteogenesis; osteoprogenitor cells; PLGA/CaP; PLGA/CaP

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Sicchieri, L. G. (2010). Engenharia de tecido ósseo: avaliações in vitro e in vivo do biomaterial híbrido ácido poli-láctico-co-glicólico/fosfato de cálcio e células osteoblásticas derivadas de células-tronco. (Masters Thesis). University of São Paulo. Retrieved from http://www.teses.usp.br/teses/disponiveis/58/58136/tde-17092010-084425/ ;

Chicago Manual of Style (16^th Edition):

Sicchieri, Luciana Gonçalves. “Engenharia de tecido ósseo: avaliações in vitro e in vivo do biomaterial híbrido ácido poli-láctico-co-glicólico/fosfato de cálcio e células osteoblásticas derivadas de células-tronco.” 2010. Masters Thesis, University of São Paulo. Accessed October 15, 2019. http://www.teses.usp.br/teses/disponiveis/58/58136/tde-17092010-084425/ ;.

MLA Handbook (7^th Edition):

Sicchieri, Luciana Gonçalves. “Engenharia de tecido ósseo: avaliações in vitro e in vivo do biomaterial híbrido ácido poli-láctico-co-glicólico/fosfato de cálcio e células osteoblásticas derivadas de células-tronco.” 2010. Web. 15 Oct 2019.

Vancouver:

Sicchieri LG. Engenharia de tecido ósseo: avaliações in vitro e in vivo do biomaterial híbrido ácido poli-láctico-co-glicólico/fosfato de cálcio e células osteoblásticas derivadas de células-tronco. [Internet] [Masters thesis]. University of São Paulo; 2010. [cited 2019 Oct 15]. Available from: http://www.teses.usp.br/teses/disponiveis/58/58136/tde-17092010-084425/ ;.

Council of Science Editors:

Sicchieri LG. Engenharia de tecido ósseo: avaliações in vitro e in vivo do biomaterial híbrido ácido poli-láctico-co-glicólico/fosfato de cálcio e células osteoblásticas derivadas de células-tronco. [Masters Thesis]. University of São Paulo; 2010. Available from: http://www.teses.usp.br/teses/disponiveis/58/58136/tde-17092010-084425/ ;

Aristotle University Of Thessaloniki (AUTH); Αριστοτέλειο Πανεπιστήμιο Θεσσαλονίκης (ΑΠΘ)

24. Katsikari, Athanasia. Ανάπτυξη μεθόδων μεταφοράς βιομορίων με τη χρήση νανοσωματιδίων.

Degree: 2014, Aristotle University Of Thessaloniki (AUTH); Αριστοτέλειο Πανεπιστήμιο Θεσσαλονίκης (ΑΠΘ)

URL: http://hdl.handle.net/10442/hedi/41653

► Several problems associated with free drugs and vaccines such as lowsolubility, poor stability and unwanted side effects, had led to developingnovel drug delivery systems. Although… (more)

▼ Several problems associated with free drugs and vaccines such as lowsolubility, poor stability and unwanted side effects, had led to developingnovel drug delivery systems. Although various innovative delivery systemshave been introduced, there still remains need for further improvement of theissues. In the past few decades particle-based delivery systems have beenenormously investigated to resolve these problems.In particular, the use of biodegradable polymeric nanoparticles withentrapped drugs or antigens represents an exciting approach for controllingthe release of drugs and vaccines. Among polymeric particles, poly (D, Llactide-co-glycolide) (PLGA)–based nanoparticles (NPs) are one of the mostfrequently studied delivery carriers for drugs, peptides, proteins, vaccines andnucleotides. PLGA is a copolymer composed of lactic acid and glycolic acidand is one of a few polymers approved for medical purposes due to itsbiodegradability and nontoxicity.The aim of the present thesis was the production of biodegradablePLGA NPs for efficient protein and DNA delivery to cells and tissues.Nanoparticles were produced by the double emulsion and solvent evaporationtechnique, after the determination of several process parameters affectingtheir physicochemical properties. The produced nanoparticles were fullycharacterized with respect to size, surface charge and morphology. Therelease profile of the encapsulated DNA, as well as the integrity of DNAextracted from nanoparticles, were also examined.The evaluation of potential NP toxicity showed that cell viability isreduced only at nanoparticles concentrations greater than 1 mg/ml. Uptakestudies revealed that nanoparticles were internalized as early as within 30minutes after incubation and that they were distributed around the cytoplasmand the peri-nuclear space. Moreover, the in vitro uptake studiesdemonstrated that cellular uptake of nanoparticles is a concentration-, timeandtemperature- dependent process and that the uptake was inhibitedsignificantly by lowering the incubation temperature at 4ºC, and by thepresence of inhibitors like sodium azide and cytochalasin D. The aboveresults suggest that uptake is an active process and that endocytosis is themain internalization mechanism of nanoparticles in vitro. After first-line in vitro assays, characterization of cellular effects by thedorsal air pouch model was straightforward. It was clearly established thatPLGA NPs possess in vivo immunomodulatory activity since they attractpolymorphonuclear cells (PMNs). Furthermore, intramuscular injection ofpMAX-GFP NPs resulted in enhanced GFP expression in quadriceps musclein Balb/c mice. Finally, it was demonstrated that after oral administration ofFITC loaded PLGA NPs in Balb/c mice nanoparticles can be detected in theintestine within 30 minutes after feeding. Significant levels of gene expressionit was also detected when mice were fed with PLGA NPs containing pMAXGFPplasmid, 12 days after the first immunization.Consequently, the results of the present doctoral thesis underline thepotential of…

Subjects/Keywords: Νανοσωματίδια PLGA; Κυτταροτοξικότητα; Πρόσληψη νανοσωματιδίων; Μεταφορά DNA; PLGA nanoparticles; Cytotoxicity; Nanoparticle uptake; DNA delivery

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Katsikari, A. (2014). Ανάπτυξη μεθόδων μεταφοράς βιομορίων με τη χρήση νανοσωματιδίων. (Thesis). Aristotle University Of Thessaloniki (AUTH); Αριστοτέλειο Πανεπιστήμιο Θεσσαλονίκης (ΑΠΘ). Retrieved from http://hdl.handle.net/10442/hedi/41653

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Katsikari, Athanasia. “Ανάπτυξη μεθόδων μεταφοράς βιομορίων με τη χρήση νανοσωματιδίων.” 2014. Thesis, Aristotle University Of Thessaloniki (AUTH); Αριστοτέλειο Πανεπιστήμιο Θεσσαλονίκης (ΑΠΘ). Accessed October 15, 2019. http://hdl.handle.net/10442/hedi/41653.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Katsikari, Athanasia. “Ανάπτυξη μεθόδων μεταφοράς βιομορίων με τη χρήση νανοσωματιδίων.” 2014. Web. 15 Oct 2019.

Vancouver:

Katsikari A. Ανάπτυξη μεθόδων μεταφοράς βιομορίων με τη χρήση νανοσωματιδίων. [Internet] [Thesis]. Aristotle University Of Thessaloniki (AUTH); Αριστοτέλειο Πανεπιστήμιο Θεσσαλονίκης (ΑΠΘ); 2014. [cited 2019 Oct 15]. Available from: http://hdl.handle.net/10442/hedi/41653.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Katsikari A. Ανάπτυξη μεθόδων μεταφοράς βιομορίων με τη χρήση νανοσωματιδίων. [Thesis]. Aristotle University Of Thessaloniki (AUTH); Αριστοτέλειο Πανεπιστήμιο Θεσσαλονίκης (ΑΠΘ); 2014. Available from: http://hdl.handle.net/10442/hedi/41653

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

25. Rigaux, Guillaume. Elaboration, caractérisation et évaluation biologique de nanoparticules biocompatibles pour la thérapie photodynamique et l’imagerie IRM : Elaboration, characterization and biological evaluation of biocompatible nanoparticles for photodynamic therapy and MRI.

Degree: Docteur es, Sciences - STS, 2015, Reims

URL: http://www.theses.fr/2015REIMS026

►

L'objectif poursuivi au cours de ce travail est l'élaboration de nanoparticules biocompatibles à visée diagnostique (IRM) et thérapeutique (PDT). Dans ce but, un protocole de… (more)

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L'objectif poursuivi au cours de ce travail est l'élaboration de nanoparticules biocompatibles à visée diagnostique (IRM) et thérapeutique (PDT). Dans ce but, un protocole de nanoprécipitation a été optimisé pour obtenir de façon quantitative et reproductible, des nanoparticules de PLGA de diamètre compatible avec une injection par voie parentérale. Cette formulation a été employée avec succès pour l'encapsulation d'un chélate lipophile de Gd(III), pour l'encapsulation d'un photosensibilisateur (m-THPC) et pour la co-encapsulation de ces deux substances actives. Les formulations optimales permettent d'obtenir des efficacités d'encapsulation de 7 et 46 % en chélate de gadolinium et m-THPC respectivement. La cytotoxicité et la photocytotoxicité des [email protected]PLGA ont été testées sur deux lignées cellulaires (C6 et fibroblastes) et les résultats obtenus montrent que les propriétés photocytotoxiques du m-THPC sont maintenues après l'encapsulation. L'efficacité IRM de ces nanoparticules a aussi été évaluée et les mesures NMRD et IRM à 3T montrent que l'encapsulation des chélates de gadolinium améliore leur capacité à générer un contraste en mode T1 et donc la qualité des images.

This work aimed at the synthesis of biocompatible nanoparticles for PDT and MRI applications. To reach this goal, a nanoprecipitation technique was optimized using only biocompatible starting materials. This technique allowed the preparation, in a reproducible and quantitative manner of PLGA nanoparticles, compatible with parenteral injections. This formulation was successfully applied to encapsulate a lipophilic Gd(III) chelate, a photosensitizer (m-THPC) and to co-encapsulate these two substances. Optimal formulations showed encapsulation yields of 7 and 46 % for the gadolinium chelate and m-THPC, respectively. Cytotoxicity and photocytotoxicity experiments performed for two different cell lines (C6 cells and fibroblasts) incubated with [email protected]PLGA nanoparticles showed that m-THPC photocytotoxicity was maintained after its encapsulation. MRI efficacy of [email protected]PLGA nanoparticles was also evaluated by NMRD measurements and 3T MRI images. The corresponding results indicated that gadolinium chelate encapsulation improved its tendency to generate an efficient T1 contrast and consequently, enhanced the image contrast.

Advisors/Committee Members: Chuburu, Françoise (thesis director).

Subjects/Keywords: Nanoparticules de PLGA; Nanoprécipitation; Photosensibilisateurs; Pdt; Gadolinium; Irm; PLGA nanoparticles; Nanoprecipitation; Photosensitizer; Pdt; Gadolinium; Mri

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Rigaux, G. (2015). Elaboration, caractérisation et évaluation biologique de nanoparticules biocompatibles pour la thérapie photodynamique et l’imagerie IRM : Elaboration, characterization and biological evaluation of biocompatible nanoparticles for photodynamic therapy and MRI. (Doctoral Dissertation). Reims. Retrieved from http://www.theses.fr/2015REIMS026

Chicago Manual of Style (16^th Edition):

Rigaux, Guillaume. “Elaboration, caractérisation et évaluation biologique de nanoparticules biocompatibles pour la thérapie photodynamique et l’imagerie IRM : Elaboration, characterization and biological evaluation of biocompatible nanoparticles for photodynamic therapy and MRI.” 2015. Doctoral Dissertation, Reims. Accessed October 15, 2019. http://www.theses.fr/2015REIMS026.

MLA Handbook (7^th Edition):

Rigaux, Guillaume. “Elaboration, caractérisation et évaluation biologique de nanoparticules biocompatibles pour la thérapie photodynamique et l’imagerie IRM : Elaboration, characterization and biological evaluation of biocompatible nanoparticles for photodynamic therapy and MRI.” 2015. Web. 15 Oct 2019.

Vancouver:

Rigaux G. Elaboration, caractérisation et évaluation biologique de nanoparticules biocompatibles pour la thérapie photodynamique et l’imagerie IRM : Elaboration, characterization and biological evaluation of biocompatible nanoparticles for photodynamic therapy and MRI. [Internet] [Doctoral dissertation]. Reims; 2015. [cited 2019 Oct 15]. Available from: http://www.theses.fr/2015REIMS026.

Council of Science Editors:

Rigaux G. Elaboration, caractérisation et évaluation biologique de nanoparticules biocompatibles pour la thérapie photodynamique et l’imagerie IRM : Elaboration, characterization and biological evaluation of biocompatible nanoparticles for photodynamic therapy and MRI. [Doctoral Dissertation]. Reims; 2015. Available from: http://www.theses.fr/2015REIMS026

Université de Lorraine

26. Riffault, Mathieu. Évaluation biologique d’un vecteur nanoparticulaire à visée ostéoarticulaire : Biological evaluation of a nanostructured vector for osteoarticular pathologies.

Degree: Docteur es, Sciences de la vie et de la santé, 2015, Université de Lorraine

URL: http://www.theses.fr/2015LORR0094

►

Un des écueils du traitement des pathologies ostéoarticulaires est l’adressage de molécules aux cellules et tissus qui constituent l’articulation. L’administration de principes actifs par voie… (more)

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Un des écueils du traitement des pathologies ostéoarticulaires est l’adressage de molécules aux cellules et tissus qui constituent l’articulation. L’administration de principes actifs par voie sanguine ou orale nécessite l’apport de quantités importantes et peut causer des effets indésirables. Dans ces travaux, nous avons évalué un système d’adressage de molécules hydrophiles. Des particules de polymère d’acide lactique et glycolique, marquées par un traceur fluorescent ont été évaluées in vitro puis in vivo. Ces particules possèdent un recouvrement en acide hyaluronique pour améliorer les interactions avec les cellules. Les études réalisées ont permis de mettre en évidence l’internalisation des particules dans les synoviocytes, chondrocytes, et cellules souches mésenchymateuses après 8 heures d’exposition. Parallèlement, l’évaluation in vitro de différents paramètres de cytotoxicité et d’inflammation a permis de souligner la compatibilité de ces vecteurs avec les cellules articulaires. Enfin, les effets de l’injection intra-articulaire de particules ont été évalués chez le rat sain et différents modèles pathologiques. L’analyse histologique des articulations a démontré l’absence de dégradation de la matrice cartilagineuse et de réaction inflammatoire suite à l’injection de particules. Il a été également constaté un ciblage majoritaire des particules vers la membrane synoviale. Ces travaux permettent de valider l’utilisation de ce type de vecteur pour le ciblage de l’articulation et l’apport de principes actifs. L’association de ces particules à des molécules hydrophiles (acides nucléiques, médicaments,…), pourra permettre leur apport direct dans l’articulation et ouvrir de nouvelles perspectives thérapeutiques.

One of the major issues with treatment of osteoarticular diseases is reaching cells or tissue inside the joint. Administration of an active compound by intravenous or per os requires elevated amount to be effective, and can initiate systemic and negative side effects. In this work we have evaluated a drug delivery system for hydrophilic molecule, which can be used for articular tissues. Polymeric nanoparticles of poly lactic-co-glycolic acid, labelled with a fluorescent dye were evaluated both in vitro and in vivo. These nanoparticles are covered with hyaluronic acid to favor particles-cells interaction. We demonstrated here that such particles are internalized after 8 hours of exposure in synoviocytes, chondrocytes and mesenchymal stem cells. Evaluation of cytotoxicity and inflammation revealed the neutrality of these particles for articular cell types. Finally, intraarticular injections of particles were performed in healthy rat joint and in pathological models. Histological analyses of extracellular matrix integrity and inflammatory status do not demonstrate any side-effect following particles injections. These healthy nano-device target primarily synovial cells, and their degradation inside cells does not provoke deleterious effects. This PLGA based drug delivery system would be used to safely deliver…

Advisors/Committee Members: Gillet, Pierre (thesis director), Grossin, Laurent (thesis director).

Subjects/Keywords: Nanoparticules; PLGA; Articulation; Acide hyaluronique; Vectorisation; Nanoparticles; PLGA; Joint; Hyaluronic acid; Vectorization; 615.7

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Riffault, M. (2015). Évaluation biologique d’un vecteur nanoparticulaire à visée ostéoarticulaire : Biological evaluation of a nanostructured vector for osteoarticular pathologies. (Doctoral Dissertation). Université de Lorraine. Retrieved from http://www.theses.fr/2015LORR0094

Chicago Manual of Style (16^th Edition):

Riffault, Mathieu. “Évaluation biologique d’un vecteur nanoparticulaire à visée ostéoarticulaire : Biological evaluation of a nanostructured vector for osteoarticular pathologies.” 2015. Doctoral Dissertation, Université de Lorraine. Accessed October 15, 2019. http://www.theses.fr/2015LORR0094.

MLA Handbook (7^th Edition):

Riffault, Mathieu. “Évaluation biologique d’un vecteur nanoparticulaire à visée ostéoarticulaire : Biological evaluation of a nanostructured vector for osteoarticular pathologies.” 2015. Web. 15 Oct 2019.

Vancouver:

Riffault M. Évaluation biologique d’un vecteur nanoparticulaire à visée ostéoarticulaire : Biological evaluation of a nanostructured vector for osteoarticular pathologies. [Internet] [Doctoral dissertation]. Université de Lorraine; 2015. [cited 2019 Oct 15]. Available from: http://www.theses.fr/2015LORR0094.

Council of Science Editors:

Riffault M. Évaluation biologique d’un vecteur nanoparticulaire à visée ostéoarticulaire : Biological evaluation of a nanostructured vector for osteoarticular pathologies. [Doctoral Dissertation]. Université de Lorraine; 2015. Available from: http://www.theses.fr/2015LORR0094

Université Paris-Sud – Paris XI

27. Giacalone, Giovanna. Implant chargé en nanoparticules pour la libération contrôlée et le ciblage lymphatique de nucléotides et d’analogues nucléotidiques : Multi-stage delivery of nucleotides and nucleotide analogs to lymph nodes and leukocytes.

Degree: Docteur es, Pharmacotechnie et biopharmacie, 2014, Université Paris-Sud – Paris XI

URL: http://www.theses.fr/2014PA114845

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Les nucléotides naturels et les analogues nucléotidiques présentent des activités pharmacologiques importantes : par exemple, le nucléotide adénosine triphosphate (ATP) présente un intérêt pour le… (more)

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Les nucléotides naturels et les analogues nucléotidiques présentent des activités pharmacologiques importantes : par exemple, le nucléotide adénosine triphosphate (ATP) présente un intérêt pour le traitement de l'ischémie ou de plaques d'athérosclérose. L'utilisation clinique de ces molécules est cependant limitée en raison de la présence d'un groupe triphosphate, qui est sujet à l'hydrolyse in vivo, et responsable de la forte hydrophilie des molécules, ce qui limite fortement leur capture par les cellules cibles et l'accès à leurs cibles pharmacologiques intracellulaires. Pour surmonter ces limitations et permettre l'administration de nucléotides et d’analogues nucléotidiques, l'utilisation de systèmes de drug delivery comme les nanoparticules pourrait assurer la protection et l'administration ciblée des molécules actives. Cependant, les nanoparticules conçues pour l’administration intraveineuse ne sont pas toujours adaptées au traitement de certaines maladies chroniques. C’est pour cela qu’un implant sous-cutané avec des caractéristiques de libération prolongée peut représenter une alternative valable, tout en étant peu invasif et capable d’atteindre les tissus lymphatiques, cible importante de plusieurs thérapies.Le premier chapitre de cette thèse porte sur la formulation de nanoparticules pour encapsuler l’ATP ou la zidovudine triphosphate (AZT-TP), grâce à la présence du chitosane (CS). Ces nanoparticules sont formées par interactions ioniques entre les charges positives du chitosane et les charges négatives des groupes triphosphates de l’ATP ou de l’AZT-TP. Dans ce travail, les nanoparticules sont caractérisées et leur délivrance cellulaire de l’ATP et de l’AZT-TP est démontrée sur une lignée cellulaire de macrophages. Dans un deuxième temps, la stabilité de ces systèmes a été améliorée afin d'obtenir un meilleur comportement en conditions physiologiques. Cette amélioration de la stabilité a été obtenue par la complexation du fer(III) au chitosane (CS-Fe). Cette stratégie a été appliquée aux nanoparticules de tripolyphosphate (TPP) et d’ATP. Les nanoparticules ont été ensuite testées sur deux lignées de cellules macrophagiques, montrant une internalisation améliorée de l’ATP par rapport aux nanoparticules précédentes. Enfin, les nanoparticules à base de CS-Fe et ATP ont été dispersées dans une solution de PLGA, dans le but de mettre au point un implant à formation in situ. Une fois en contact avec les fluides physiologiques, la suspension prend la forme d’un dépôt solide. Des études de libération in vitro montrent la capacité des systèmes de retenir les nanoparticules à l’intérieur de la matrice et de les libérer de façon progressive pendant 5 jours. Après administration sous-cutanée chez la souris, les implants de PLGA contenant les nanoparticules ont retenu l’ATP au lieu de l’injection jusqu’à 50 heures, comparé à quelques heures pour l’ATP libre et les nanoparticules libres, montrant ainsi leur pertinence comme systèmes pour la libération prolongée de nucléotides.

Natural nucleotides and nucleotide…

Advisors/Committee Members: Fattal, Elias (thesis director), Hillaireau, Hervé (thesis director).

Subjects/Keywords: Nanoparticules; ATP; Chitosane; Libération prolongée; PLGA; Vectorisation; Nanoparticles; ATP; Chitosan; PLGA; Sustained release; Drug delivery

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Giacalone, G. (2014). Implant chargé en nanoparticules pour la libération contrôlée et le ciblage lymphatique de nucléotides et d’analogues nucléotidiques : Multi-stage delivery of nucleotides and nucleotide analogs to lymph nodes and leukocytes. (Doctoral Dissertation). Université Paris-Sud – Paris XI. Retrieved from http://www.theses.fr/2014PA114845

Chicago Manual of Style (16^th Edition):

Giacalone, Giovanna. “Implant chargé en nanoparticules pour la libération contrôlée et le ciblage lymphatique de nucléotides et d’analogues nucléotidiques : Multi-stage delivery of nucleotides and nucleotide analogs to lymph nodes and leukocytes.” 2014. Doctoral Dissertation, Université Paris-Sud – Paris XI. Accessed October 15, 2019. http://www.theses.fr/2014PA114845.

MLA Handbook (7^th Edition):

Giacalone, Giovanna. “Implant chargé en nanoparticules pour la libération contrôlée et le ciblage lymphatique de nucléotides et d’analogues nucléotidiques : Multi-stage delivery of nucleotides and nucleotide analogs to lymph nodes and leukocytes.” 2014. Web. 15 Oct 2019.

Vancouver:

Giacalone G. Implant chargé en nanoparticules pour la libération contrôlée et le ciblage lymphatique de nucléotides et d’analogues nucléotidiques : Multi-stage delivery of nucleotides and nucleotide analogs to lymph nodes and leukocytes. [Internet] [Doctoral dissertation]. Université Paris-Sud – Paris XI; 2014. [cited 2019 Oct 15]. Available from: http://www.theses.fr/2014PA114845.

Council of Science Editors:

Giacalone G. Implant chargé en nanoparticules pour la libération contrôlée et le ciblage lymphatique de nucléotides et d’analogues nucléotidiques : Multi-stage delivery of nucleotides and nucleotide analogs to lymph nodes and leukocytes. [Doctoral Dissertation]. Université Paris-Sud – Paris XI; 2014. Available from: http://www.theses.fr/2014PA114845

University of Illinois – Urbana-Champaign

28. Stovall, Kalena D. Investigation of properties affecting controlled release of macromolecules from PLGA microspheres.

Degree: PhD, 0300, 2010, University of Illinois – Urbana-Champaign

URL: http://hdl.handle.net/2142/14576

► Poly(lactide-co-glycolide), or PLGA, microspheres offer a widely-studied biodegradable option for controlled release of therapeutics. An array of fabrication methodologies have been developed to produce these… (more)

▼ Poly(lactide-co-glycolide), or PLGA, microspheres offer a widely-studied biodegradable option for controlled release of therapeutics. An array of fabrication methodologies have been developed to produce these microspheres with the capacity to encapsulate therapeutics of various types; and produce microspheres of a wide range of sizes for different methods of delivery. The encapsulation, stability, and release profiles of therapeutic release based on physical and thermodynamic properties has also been studied and modeled to an extent. Much research has been devoted to tailoring formulations for improved therapeutic encapsulation and stability as well as selective release profiles. Despite the breadth of available research on PLGA microspheres, further analysis of fundamental principles regarding the microsphere degradation, formation, and therapeutic encapsulation is necessary. This work aims to examine additional fundamental principles related to PLGA microsphere formation and degradation from solvent-evaporation of preformed polymer. In particular, mapping the development of the acidic microenvironment inside the microsphere during degradation and erosion is discussed. Also, the effect of macromolecule size and conformation is examined with respect to microsphere diameter and PLGA molecular weight. Lastly, the effects of mechanical shearing and protein exposure to aqueous media during microsphere formation are examined. In an effort to better understand the acidic microenvironment development across the microsphere diameter, pH sensitive dye conjugated to protein that undergoes conformational change at different acidic pH values was encapsulated in PLGA microspheres of diameters ranging from 40 µm to 80 µm, and used in conjunction with fluorescence resonance energy transfer to measure the radial pH change in the microspheres. Qualitative analysis of confocal micrographs was used to correlate fluorescence intensity with pH value, and obtain the radial pH across the center of the microsphere. Therapeutic encapsulation and release from polymeric microspheres is governed by an interconnected variety of factors, including the therapeutic itself. The globular protein bovine serum albumin, and the elongated and significantly smaller enzyme, lysozyme, were encapsulated in PLGA microspheres ranging from 40 µm to 80 µm in diameter. The initial surface morphology upon microsphere formation, release profiles, and microsphere erosion characteristics were explored in an effort to better understand the effect of protein size, conformation, and known PLGA interaction on the formation and degradation of PLGA microspheres and macromolecule release, with respect to PLGA molecular weight and microsphere diameter. In addition to PLGA behavior and macromolecule behavior, the effect of mechanical stresses during fabrication was examined. Two similar solvent extraction techniques were compared for the fabrication of albumin loaded microspheres. In particular, the homogeneity of the microspheres as well as capacity to retain… Advisors/Committee Members: Pack, Daniel W. (advisor), Pack, Daniel W. (Committee Chair), Braatz, Richard D. (committee member), Chen, Jianjun (committee member), Higdon, Jonathan J.L. (committee member).

Subjects/Keywords: Microspheres; controlled release; precision fabrication; Poly(lactide-co-glycolide) (PLGA); macromolecule release; PLGA microspheres

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Stovall, K. D. (2010). Investigation of properties affecting controlled release of macromolecules from PLGA microspheres. (Doctoral Dissertation). University of Illinois – Urbana-Champaign. Retrieved from http://hdl.handle.net/2142/14576

Chicago Manual of Style (16^th Edition):

Stovall, Kalena D. “Investigation of properties affecting controlled release of macromolecules from PLGA microspheres.” 2010. Doctoral Dissertation, University of Illinois – Urbana-Champaign. Accessed October 15, 2019. http://hdl.handle.net/2142/14576.

MLA Handbook (7^th Edition):

Stovall, Kalena D. “Investigation of properties affecting controlled release of macromolecules from PLGA microspheres.” 2010. Web. 15 Oct 2019.

Vancouver:

Stovall KD. Investigation of properties affecting controlled release of macromolecules from PLGA microspheres. [Internet] [Doctoral dissertation]. University of Illinois – Urbana-Champaign; 2010. [cited 2019 Oct 15]. Available from: http://hdl.handle.net/2142/14576.

Council of Science Editors:

Stovall KD. Investigation of properties affecting controlled release of macromolecules from PLGA microspheres. [Doctoral Dissertation]. University of Illinois – Urbana-Champaign; 2010. Available from: http://hdl.handle.net/2142/14576

29. Albert, Claire. Émulsions de Pickering biodégradables stabilisées par des nanoparticules de poly(acide lactique-co-glycolique) : étude physico-chimique et potentialité pharmaceutique : Biodegradable Pickering emulsions stabilized with poly(lactic-co-glycolic acid) nanoparticles : physico-chemical study and pharmaceutical potentiality.

Degree: Docteur es, Pharmacotechnie et biopharmacie, 2017, Paris Saclay

URL: http://www.theses.fr/2017SACLS491

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Dans ce travail de thèse, nous avons formulé des émulsions de Pickering stables, biodégradables et biocompatibles stabilisées par des nanoparticules (NPs) de poly(acide lactique-co-glycolique) (PLGA).… (more)

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Dans ce travail de thèse, nous avons formulé des émulsions de Pickering stables, biodégradables et biocompatibles stabilisées par des nanoparticules (NPs) de poly(acide lactique-co-glycolique) (PLGA). De telles émulsions sont une alternative, potentiellement moins toxique et irritante, aux émulsions conventionnelles stabilisées par des tensioactifs synthétiques. Dans un premier temps, une étude physico-chimique approfondie de ces systèmes a permis de clarifier leurs structures (macroscopique, microscopique et interfaciale) ainsi que leurs mécanismes et leurs cinétiques de stabilisation. Des études de la contribution du polymère stabilisant les NPs et des caractéristiques du polymère de PLGA utilisé sur les propriétés des émulsions ont également été réalisées. Cela a permis de mieux identifier les paramètres physico-chimiques clés nécessaires à une bonne stabilisation. Dans un second temps, nous nous sommes intéressés au potentiel pharmaceutique de ces émulsions pour une application topique. Des substances actives (SA), utilisées pour le traitement du psoriasis, ont été encapsulées avec succès dans les NPs (ciclosporine A et tacrolimus) et les gouttelettes de l’émulsion (calcitriol). Cette étude est un premier pas vers l’utilisation de ces émulsions pour la co-encapsulation de deux SA dans la même formulation : une première dans les NPs et une seconde dans les gouttelettes d’huile. La co-encapsulation devrait permettre d’améliorer l’observance du patient et pourrait conduire à un effet synergique entre les deux SA.

In this thesis work, we formulated stable, biodegradable and biocompatible Pickering emulsions stabilized with nanoparticles (NPs) of poly(lactic-co-glycolic acid) (PLGA). Such emulsions are an alternative, potentially less toxic and irritating, to conventional emulsions stabilized with surfactants. Firstly, a thorough physico-chemical study of these systems was conducted in order to clarify their structures (macroscopic, microscopic and interfacial) as well as their mechanisms and kinetics of stabilization. Studies of the contribution of the polymer stabilizing the NPs and of the characteristics of the PLGA polymer on the properties of the emulsions were also carried out. This enabled a better identification of the physico-chemical key parameters responsible for a good stabilization. Secondly, we focused on the pharmaceutical potential of these emulsions for a topical application. Pharmaceutical active ingredients (API), used for the treatment of psoriasis, were successfully encapsulated in the NPs (cyclosporine A and tacrolimus) and the emulsion droplets (calcitriol). This study is a first step towards the use of these emulsions for the co-encapsulation of two API: one in the NPs and a second in the oil droplets. The co-encapsulation should improve patient compliance and could lead to a synergistic effect between the two API.

Advisors/Committee Members: Agnely, Florence (thesis director), Huang, Nicolas (thesis director).

Subjects/Keywords: Émulsion; Nanoparticule; Plga; Interface; Microstructure; Encapsulation; Emulsion; Nanoparticle; Plga; Interface; Microstructure; Encapsulation

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Albert, C. (2017). Émulsions de Pickering biodégradables stabilisées par des nanoparticules de poly(acide lactique-co-glycolique) : étude physico-chimique et potentialité pharmaceutique : Biodegradable Pickering emulsions stabilized with poly(lactic-co-glycolic acid) nanoparticles : physico-chemical study and pharmaceutical potentiality. (Doctoral Dissertation). Paris Saclay. Retrieved from http://www.theses.fr/2017SACLS491

Chicago Manual of Style (16^th Edition):

Albert, Claire. “Émulsions de Pickering biodégradables stabilisées par des nanoparticules de poly(acide lactique-co-glycolique) : étude physico-chimique et potentialité pharmaceutique : Biodegradable Pickering emulsions stabilized with poly(lactic-co-glycolic acid) nanoparticles : physico-chemical study and pharmaceutical potentiality.” 2017. Doctoral Dissertation, Paris Saclay. Accessed October 15, 2019. http://www.theses.fr/2017SACLS491.

MLA Handbook (7^th Edition):

Albert, Claire. “Émulsions de Pickering biodégradables stabilisées par des nanoparticules de poly(acide lactique-co-glycolique) : étude physico-chimique et potentialité pharmaceutique : Biodegradable Pickering emulsions stabilized with poly(lactic-co-glycolic acid) nanoparticles : physico-chemical study and pharmaceutical potentiality.” 2017. Web. 15 Oct 2019.

Vancouver:

Albert C. Émulsions de Pickering biodégradables stabilisées par des nanoparticules de poly(acide lactique-co-glycolique) : étude physico-chimique et potentialité pharmaceutique : Biodegradable Pickering emulsions stabilized with poly(lactic-co-glycolic acid) nanoparticles : physico-chemical study and pharmaceutical potentiality. [Internet] [Doctoral dissertation]. Paris Saclay; 2017. [cited 2019 Oct 15]. Available from: http://www.theses.fr/2017SACLS491.

Council of Science Editors:

Albert C. Émulsions de Pickering biodégradables stabilisées par des nanoparticules de poly(acide lactique-co-glycolique) : étude physico-chimique et potentialité pharmaceutique : Biodegradable Pickering emulsions stabilized with poly(lactic-co-glycolic acid) nanoparticles : physico-chemical study and pharmaceutical potentiality. [Doctoral Dissertation]. Paris Saclay; 2017. Available from: http://www.theses.fr/2017SACLS491

30. Pereira, Priscilla Aparecida Tartari. Efeitos antagônicos da prostaglandina D2 e prostaglandina E2 na resposta imune durante infecção experimental por Histoplasma capsulatum.

Degree: PhD, Imunologia Básica e Aplicada, 2013, University of São Paulo

URL: http://www.teses.usp.br/teses/disponiveis/17/17147/tde-06012014-081806/ ;

► O Histoplasma capsulatum é um fungo dimórfico, patogênico e responsável por graves lesões pulmonares. A infecção é adquirida pela inalação de conídios e posterior conversão… (more)

▼ O Histoplasma capsulatum é um fungo dimórfico, patogênico e responsável por graves lesões pulmonares. A infecção é adquirida pela inalação de conídios e posterior conversão para leveduras nos alvéolos e bronquíolos, onde são fagocitadas por macrófagos alveolares residentes e leucócitos que migram para o local da infecção. Recentemente, demonstramos que animais infectados com H. capsulatum e tratados com inibidor da síntese de prostaglandinas apresentaram diminuição de carga fúngica nos pulmões e baço, aumento da produção de nitrito e da fagocitose de leveduras por macrófagos alveolares, e maior sobrevivência, quando comparados com os animais somente infectados. Porém, neste estudo não foram determinados quais subtipos de prostaglandinas participam na patogênese da histoplasmose. Vários grupos de pesquisa têm demonstrado que PGD2 e PGE2 podem ter ações biológicas distintas quanto à remoção de microrganismos no hospedeiro. Desta maneira, é fundamental o entendimento do papel da PGD2 e da PGE2 nos mecanismos efetores dos macrófagos na defesa do hospedeiro, especialmente na histoplasmose. Portanto, o objetivo deste estudo foi investigar a participação da PGD2 e PGE2 na infecção experimental por H. capsulatum. Assim, demonstramos que a PGD2 aumentou a fagocitose e mecanismos microbicidas de macrófagos alveolares infectados in vitro com H. capsulatum. Observamos ainda que a 15dPGJ2, metabólito da PGD2, aumentou somente a fagocitose, e PGE2 inibiu os mecanismos efetores do macrófago. Mostramos ainda o aumento de BLT1 em macrófagos alveolares após adição de PGD2, e a possível ligação desta ao BLT1, e de LTB4 em DP2. Além disso, caracterizamos micropartículas de PLGA contendo PGD2 (MS-PGD2), e investigamos seus efeitos. O tamanho, carga elétrica e morfologia das micropartículas foram adequados para um tratamento intranasal e para fagocitose por macrófagos alveolares. As MS-PGD2 foram fagocitadas e capazes de ativar NF-B, e consequentemente, influenciar na produção de nitrito, IL-1, TNF-, IL-6 e TGF-. Com base nestes dados, avaliamos os efeitos do tratamento da MS-PGD2 ou da MS-PGE2 em animais infectados com H. capsulatum. Estas foram administradas via intranasal em animais infectados e tratados ou não com celecoxibe. Verificamos a diminuição da carga fúngica nos pulmões e baço, diminuição do infiltrador celular no espaço broncoalveolar e de citocinas inflamatórias no pulmão após tratamento com MS-PGD2. Contrariamente, após tratamento da MS-PGE2 observamos maior carga fúngica nos pulmões e baço, e aumento da inflamação no tecido e maior produção de IL-10. Além disso, demonstramos que no 21° dia após infecção, referente ao 7° dia após o término do tratamento com MS-PGD2, a carga fúngica manteve-se reduzida nos pulmões, comprovando assim a eficácia deste tratamento. Posteriormente, utilizando inibidores específicos, HQL-79 e CAY10526, mostramos respectivamente o papel protetor da PGD2 e o deletério da PGE2 na histoplasmose. Em conjunto, nossos dados contribuíram para o entendimento das funções antagônicas da PGD2 e PGE2 nesta… Advisors/Committee Members: Faccioli, Lucia Helena.

Subjects/Keywords: Alveolar macrophage; Histoplasma capsulatum; Histoplasma capsulatum; Macrófago alveolar; PLGA; PLGA; Prostaglandin D2; Prostaglandin E2; Prostaglandina D2; Prostaglandina E2

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Pereira, P. A. T. (2013). Efeitos antagônicos da prostaglandina D2 e prostaglandina E2 na resposta imune durante infecção experimental por Histoplasma capsulatum. (Doctoral Dissertation). University of São Paulo. Retrieved from http://www.teses.usp.br/teses/disponiveis/17/17147/tde-06012014-081806/ ;

Chicago Manual of Style (16^th Edition):

Pereira, Priscilla Aparecida Tartari. “Efeitos antagônicos da prostaglandina D2 e prostaglandina E2 na resposta imune durante infecção experimental por Histoplasma capsulatum.” 2013. Doctoral Dissertation, University of São Paulo. Accessed October 15, 2019. http://www.teses.usp.br/teses/disponiveis/17/17147/tde-06012014-081806/ ;.

MLA Handbook (7^th Edition):

Pereira, Priscilla Aparecida Tartari. “Efeitos antagônicos da prostaglandina D2 e prostaglandina E2 na resposta imune durante infecção experimental por Histoplasma capsulatum.” 2013. Web. 15 Oct 2019.

Vancouver:

Pereira PAT. Efeitos antagônicos da prostaglandina D2 e prostaglandina E2 na resposta imune durante infecção experimental por Histoplasma capsulatum. [Internet] [Doctoral dissertation]. University of São Paulo; 2013. [cited 2019 Oct 15]. Available from: http://www.teses.usp.br/teses/disponiveis/17/17147/tde-06012014-081806/ ;.

Council of Science Editors:

Pereira PAT. Efeitos antagônicos da prostaglandina D2 e prostaglandina E2 na resposta imune durante infecção experimental por Histoplasma capsulatum. [Doctoral Dissertation]. University of São Paulo; 2013. Available from: http://www.teses.usp.br/teses/disponiveis/17/17147/tde-06012014-081806/ ;

Open Access Theses and Dissertations