Advanced search options

Advanced Search Options 🞨

Browse by author name (“Author name starts with…”).

Find ETDs with:

in
/  
in
/  
in
/  
in

Written in Published in Earliest date Latest date

Sorted by

Results per page:

You searched for subject:(PAT Seq). One record found.

Search Limiters

Last 2 Years | English Only

No search limiters apply to these results.

▼ Search Limiters


University of Kentucky

1. Sanchez Barrios, Andrea Marisa. BACTERIAL INOCULANTS, ENDOPHYTIC BACTERIA AND THEIR INFLUENCE ON NICOTIANA PHYSIOLOGY, DEVELOPMENT AND MICROBIOME.

Degree: 2018, University of Kentucky

Soil and root microbial communities have been studied for decades, and the incorporation of high-throughput techniques and analysis has allowed the identification of endophytic/non-culturable organisms. This has helped characterize and establish the core microbiome of many model plant species which include underground and aboveground organs. Unfortunately, the information obtained from some of these model plants is not always transferable to other agronomic species. In this project, we decided to study the microbiome of the Nicotiana genus because of its importance in plant physiological and plant-microbe interactions studies. The data obtained was used as baseline information that allowed us to better understand the effect of microbial inoculums on the assembly of the microbiome of the plant. We analyzed 16s rRNA amplicons to survey the microbiome in different plant organs and rhizosphere from four different species. Bacterial strains evaluated were screened for a consistent reduction or improvement in plant growth. Four bacterial strains were tested and used as seed inoculum (Lf-Lysinobacillus fusisormis, Ms –Micrococcus sp., Bs–Bacillus sp., Bc–Bacillus cereus). Bs and Bc inoculants caused plant growth promotion, and in contrast Ms caused retarded growth, while Lf acted as a neutral or non-inducing phenotype strain. Data supported that microbial inoculum used as seed treatment caused systemic changes in the host plant microbiome. Functionality of the inoculum was studied and the response in plant growth was linked to hormonal changes (evaluated in the plant and in the bacterial strains). Gene expression analysis using a genome-scale approach revealed that genes that could possibly be involved in stress response are down-regulated for Bc and Bs treatments and up-regulated for Ms. Flexibility variability of the inoculum was also evaluated to have a better understanding of the main factors involved in the promotion or suppression of growth, and possibly its effect in following generations. In summary, the findings of this project support that the plant functional microbiome responds to exogenous stimulation from abiotic and biotic factors by adapting endogenous hormone responses.

Subjects/Keywords: Nicotiana benthamiana; microbiome; core; inoculums; morphological traits; PAT-Seq; high-throughput; hormones; Agriculture; Computational Biology; Genetics and Genomics; Microbiology; Plant Biology

Record DetailsSimilar RecordsGoogle PlusoneFacebookTwitterCiteULikeMendeleyreddit

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6th Edition):

Sanchez Barrios, A. M. (2018). BACTERIAL INOCULANTS, ENDOPHYTIC BACTERIA AND THEIR INFLUENCE ON NICOTIANA PHYSIOLOGY, DEVELOPMENT AND MICROBIOME. (Doctoral Dissertation). University of Kentucky. Retrieved from https://uknowledge.uky.edu/pss_etds/101

Chicago Manual of Style (16th Edition):

Sanchez Barrios, Andrea Marisa. “BACTERIAL INOCULANTS, ENDOPHYTIC BACTERIA AND THEIR INFLUENCE ON NICOTIANA PHYSIOLOGY, DEVELOPMENT AND MICROBIOME.” 2018. Doctoral Dissertation, University of Kentucky. Accessed September 18, 2019. https://uknowledge.uky.edu/pss_etds/101.

MLA Handbook (7th Edition):

Sanchez Barrios, Andrea Marisa. “BACTERIAL INOCULANTS, ENDOPHYTIC BACTERIA AND THEIR INFLUENCE ON NICOTIANA PHYSIOLOGY, DEVELOPMENT AND MICROBIOME.” 2018. Web. 18 Sep 2019.

Vancouver:

Sanchez Barrios AM. BACTERIAL INOCULANTS, ENDOPHYTIC BACTERIA AND THEIR INFLUENCE ON NICOTIANA PHYSIOLOGY, DEVELOPMENT AND MICROBIOME. [Internet] [Doctoral dissertation]. University of Kentucky; 2018. [cited 2019 Sep 18]. Available from: https://uknowledge.uky.edu/pss_etds/101.

Council of Science Editors:

Sanchez Barrios AM. BACTERIAL INOCULANTS, ENDOPHYTIC BACTERIA AND THEIR INFLUENCE ON NICOTIANA PHYSIOLOGY, DEVELOPMENT AND MICROBIOME. [Doctoral Dissertation]. University of Kentucky; 2018. Available from: https://uknowledge.uky.edu/pss_etds/101

.