subject:(Oxidative stress)

Oregon State University

1. Barker, Tyler. Oxidative stress and muscle dysfunction following anterior cruciate ligament surgery.

Degree: PhD, Exercise and Sports Science, 2009, Oregon State University

URL: http://hdl.handle.net/1957/11289

► Despite the advances in surgery, physical therapy, and pharmaceutical agents, muscle dysfunction (i.e., atrophy and weakness) continues to impair recovery from an anterior cruciate ligament… (more)

▼ Despite the advances in surgery, physical therapy, and pharmaceutical agents, muscle dysfunction (i.e., atrophy and weakness) continues to impair recovery from an anterior cruciate ligament (ACL) injury and surgery. Ischemia-reperfusion injury during surgery and the subsequent limb disuse are two events experienced by patients having ACL surgery. Oxidative stress and inflammation mediate muscle dysfunction; both of which can be modulated by antioxidants. The purpose of this project was to test the hypothesis that vitamin E and C supplementation would ameliorate muscle dysfunction following ACL surgery by attenuating the increase in mediators of muscle dysfunction. A randomized, double-blind, placebo-controlled study was conducted in men who received one of two supplements: 1) antioxidant (AO; 400 IU of vitamin E and 1000 mg vitamin C per day), or 2) matching placebo (PL) starting ~2-weeks prior to (baseline) and concluding 3-months post-surgery. Lower limb strength and skeletal muscle fiber cross-sectional area measurements were used to assess muscle dysfunction; markers of oxidative stress and inflammation were evaluated in the circulation and muscle. Plasma α-tocopherol (α-T) and ascorbic acid (AA) increased, while γ-T concentrations decreased significantly with AO supplementation. Following surgery, oxidative stress (F₂-isoprostanes), inflammation and muscle damage increased significantly in both groups. Compared to the PL group, AO supplementation ameliorated the increase in an anti-inflammatory cytokine (interleukin (IL)-10) and the depression of a pro- to anti-inflammatory cytokine ratio (IL-6:IL-10) immediately following surgery. Elevated AA decreased in the AO group and inversely correlated with a neutrophil chemoattractant cytokine immediately following surgery. In contrast to our expectations, a significant atrophy response from pre- to post-surgery was not observed in muscle biopsies, using histologic techniques. In fact, AO supplementation increased inducible nitric oxide synthase and myeloperoxidase expression in the muscle following surgery. Furthermore, and unlike the PL group, the recovery in peak isometric force of the injured limb within the AO group did not significantly increase from baseline to 3-months post-surgery. In summary, AO supplementation protected against immuno-suppression (increase in anti-inflammatory cytokines), but was ineffective in lowering oxidative stress induced by surgery. Moreover, AO supplementation did not minimize, and potentially contributed to muscle dysfunction following ACL reconstructive surgery. Advisors/Committee Members: Traber, Maret (advisor), Manore, Melinda (committee member).

Subjects/Keywords: Oxidative Stress; Oxidative stress

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APA (6^th Edition):

Barker, T. (2009). Oxidative stress and muscle dysfunction following anterior cruciate ligament surgery. (Doctoral Dissertation). Oregon State University. Retrieved from http://hdl.handle.net/1957/11289

Chicago Manual of Style (16^th Edition):

Barker, Tyler. “Oxidative stress and muscle dysfunction following anterior cruciate ligament surgery.” 2009. Doctoral Dissertation, Oregon State University. Accessed March 02, 2021. http://hdl.handle.net/1957/11289.

MLA Handbook (7^th Edition):

Barker, Tyler. “Oxidative stress and muscle dysfunction following anterior cruciate ligament surgery.” 2009. Web. 02 Mar 2021.

Vancouver:

Barker T. Oxidative stress and muscle dysfunction following anterior cruciate ligament surgery. [Internet] [Doctoral dissertation]. Oregon State University; 2009. [cited 2021 Mar 02]. Available from: http://hdl.handle.net/1957/11289.

Council of Science Editors:

Barker T. Oxidative stress and muscle dysfunction following anterior cruciate ligament surgery. [Doctoral Dissertation]. Oregon State University; 2009. Available from: http://hdl.handle.net/1957/11289

Oregon State University

2. Muniz, Juan Fermin. Biomarkers of oxidative stress and DNA damage in agricultural workers.

Degree: PhD, Toxicology, 2009, Oregon State University

URL: http://hdl.handle.net/1957/13998

► Pesticides are among the most pervasive environmental contaminants and they are an important potential risk for human health. Agricultural workers are constantly exposed to pesticide… (more)

▼ Pesticides are among the most pervasive environmental contaminants and they are an important potential risk for human health. Agricultural workers are constantly exposed to pesticide spray, drift and residues in the soil and foliage. Many agricultural pesticides are readily absorbed by the body, through contact with the skin, the respiratory track, the eyes, and the gastrointestinal system. Multiple studies have reported a strong association between pesticide exposure and various health outcomes including cancer. Oxidative stress and DNA damage have been proposed as mechanisms linking pesticide exposure to health effects and neurological diseases. The focus of the present translational study is to examine the relationship between human exposure to the organophosphate pesticide azinphos methyl (AZM) and oxidative stress by measuring biomarkers of oxidative stress in biological fluids (i.e., urine, serum) and peripheral blood lymphocytes (PBLs) of agricultural workers. The findings from these field studies will be validated in vitro by examining cultures of human lymphocytes treated with AZM for similar biomarkers of oxidative stress. Since the collection of PBLs from study participants is highly invasive and not suitable for studies involving younger subjects, we also examined buccal cells for biomarkers of oxidative stress (i.e., DNA damage) as a more universal source of human tissue to assess oxidative stress in pesticide exposed individuals. We demonstrated in this study that AZM induces oxidative stress and causes DNA damage in human tissues. Agricultural workers who had been exposed to AZM showed elevated serum levels of lipid peroxides, increased urinary levels of 8-OH-dG, and lymphocytes from these individuals showed increased DNA damage and associated changes in oxidative DNA repair enzymes. Biomarkers of oxidative stress were also elevated in human lymphocytes treated with physiologically relevant concentrations of AZM. In cultures of human lymphocytes, AZM caused a concentration-dependent loss of viability and associated increases in ROS and a reduction in intracellular GSH. We also demonstrated that viable leukocytes from the oral cavity can be readily obtained from humans and these buccal cells can be used to assess DNA damage following exposure to occupational and environmental genotoxicants. We also noted that oral leukocytes are especially sensitive to cryopreservation with DMSO and thus, these cells must be cryoprotected with 5% DMSO to preserve the viability of these cells for subsequent biochemical studies. In summary, these in vivo and in vitro studies demonstrated that AZM induces oxidative stress in a dose-dependent matter and that oral lymphocytes are a good source of human tissue for assessing DNA damage and possibly other biochemical changes. The possible health implications of the variations in these biomarkers of oxidative stress and DNA damage are undetermined. Yet the findings from these studies have provided a strong foundation for determining the mechanism by which pesticide induce… Advisors/Committee Members: Kisby, Glen E. (advisor), Curtis, Larry (committee member).

Subjects/Keywords: biomarkers of oxidative stress; Oxidative stress

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APA (6^th Edition):

Muniz, J. F. (2009). Biomarkers of oxidative stress and DNA damage in agricultural workers. (Doctoral Dissertation). Oregon State University. Retrieved from http://hdl.handle.net/1957/13998

Chicago Manual of Style (16^th Edition):

Muniz, Juan Fermin. “Biomarkers of oxidative stress and DNA damage in agricultural workers.” 2009. Doctoral Dissertation, Oregon State University. Accessed March 02, 2021. http://hdl.handle.net/1957/13998.

MLA Handbook (7^th Edition):

Muniz, Juan Fermin. “Biomarkers of oxidative stress and DNA damage in agricultural workers.” 2009. Web. 02 Mar 2021.

Vancouver:

Muniz JF. Biomarkers of oxidative stress and DNA damage in agricultural workers. [Internet] [Doctoral dissertation]. Oregon State University; 2009. [cited 2021 Mar 02]. Available from: http://hdl.handle.net/1957/13998.

Council of Science Editors:

Muniz JF. Biomarkers of oxidative stress and DNA damage in agricultural workers. [Doctoral Dissertation]. Oregon State University; 2009. Available from: http://hdl.handle.net/1957/13998

University of Adelaide

3. Dolman, Fleur Catherine. Functional characterisation of plant cytosolic thioredoxins.

Degree: 2010, University of Adelaide

URL: http://hdl.handle.net/2440/64583

► Thioredoxins are small, ubiquitous, disulfide oxidoreductase proteins characterised by a conserved dicysteine active site. Within the cell, they are believed to maintain the redox environment… (more)

▼ Thioredoxins are small, ubiquitous, disulfide oxidoreductase proteins characterised by a conserved dicysteine active site. Within the cell, they are believed to maintain the redox environment and participate in a broad range of biochemical processes. Plant thioredoxins are a diverse multigene family, primarily classified according to the system by which they are reduced and their subcellular localization. Thioredoxins located in the cytoplasm (type -h) are usually dependent on NADPH for reduction by NADPH-thioredoxin reductase. There are four cytosolic thioredoxins in grass species, with subclass 4 believed to be the most ancient. The highly conserved nature of thioredoxin-h4, in plant species as diverse as angiosperms and gymnosperms, implies a conservation of gene function. Discovery of thioredoxin-h4 function in barley (Hordeum vulgare) was the core focus of the research presented in this thesis. The characterisation of thioredoxin-h4 was approached from both, genetic, and protein biochemistry perspectives. To commence the research, the transcript profile of barley thioreodoxin-h4 (HvTrx-h4) was examined in barley reproductive tissues. As a direct consequence of findings, anther and stigma tissues were used in protein interaction studies employing a mono-cystenic active-site HvTrx-h4 affinity chromatography technique. HvTrx-h4 was mutated, recombinantly expressed, purified and immobilised in order to isolate and identify proteins with which it interacted. Identification of HvTrx-h4 protein targets sought to reveal the pathways in which thioredoxin-h4 is involved. To further characterise the expression of HvTrx-h4, the promoter and 5′ untranslated regions of genomic sequence were isolated and used to drive expression of green fluorescent protein in transgenically modified barley. This enabled examination of the temporal and spatial regulation of HvTrx-h4 under normal growth conditions, as well as in response to abiotic stress and plant hormone treatments. Through these studies it was discovered that HvTrx-h4 is likely to be the subject of post-transcriptional modifications. Subsequent investigations revealed HvTrx-h4 is also regulated at the post-translational level through glutathionylation. Previous studies have ascribed a role for thioredoxins in plant oxidative stress defence. The question of whether modulation of HvTrx-h4 expression could be manipulated to alter plant oxidative stress tolerance was considered. To investigate, transgenic tobacco plants (Nicotiana tabacum) containing altered amounts of thioredoxin-h4 protein were subjected to various stresses; abiotic, biotic and chemical, in nature. Tobacco constitutively over-expressing thioredoxin-h4 displayed increased tolerance to ultraviolet light B, heat and methyl viologen treatment. Knowledge acquired by this study and presented in this thesis, suggest a role for barley thioredoxin-h4 in the oxidative stress response. Furthermore, the description of both post-transcriptional and post-translational regulation of HvTrx-h4 constitutes the first report of… Advisors/Committee Members: Baumann, Ute (advisor), Juttner, Juan Antony (advisor), Comis, Alfio (advisor), School of Agriculture, Food and Wine (school).

Subjects/Keywords: thioredoxin; oxidative stress

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APA (6^th Edition):

Dolman, F. C. (2010). Functional characterisation of plant cytosolic thioredoxins. (Thesis). University of Adelaide. Retrieved from http://hdl.handle.net/2440/64583

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Dolman, Fleur Catherine. “Functional characterisation of plant cytosolic thioredoxins.” 2010. Thesis, University of Adelaide. Accessed March 02, 2021. http://hdl.handle.net/2440/64583.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Dolman, Fleur Catherine. “Functional characterisation of plant cytosolic thioredoxins.” 2010. Web. 02 Mar 2021.

Vancouver:

Dolman FC. Functional characterisation of plant cytosolic thioredoxins. [Internet] [Thesis]. University of Adelaide; 2010. [cited 2021 Mar 02]. Available from: http://hdl.handle.net/2440/64583.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Dolman FC. Functional characterisation of plant cytosolic thioredoxins. [Thesis]. University of Adelaide; 2010. Available from: http://hdl.handle.net/2440/64583

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Rochester

4. Rahman, Mohammed Mahidur. The Role of the Nrf2 Dimerization Partner Maf-S in Drosophila Gene Regulation, Stress Response and Aging.

Degree: PhD, 2012, University of Rochester

URL: http://hdl.handle.net/1802/25519

► Aging is an unavoidable and universal process of physiological and physical decline of living beings. It is associated with declining ability to perform basic functions,… (more)

▼ Aging is an unavoidable and universal process of physiological and physical decline of living beings. It is associated with declining ability to perform basic functions, resist stress and various other deteriorating conditions. According to the oxidative stress hypothesis of aging, oxidative damage is causative for the process of aging and exacerbates age-associated pathologies. The transcription factor Nrf2 is a major regulator of anti-oxidant, detoxification and stress defense genes. Several studies have demonstrated that Nrf2 signaling can counteract a number of age-associated pathologies and that the activation of Nrf2 can promote longevity in model organisms. Studies in our laboratory established that the antioxidant Nrf2 pathway is functionally and structurally conserved in the Drosophila melanogaster. It was shown that the protein product of the cap’n’collar, splice form C or CncC is the ortholog of mammalian Nrf2. Furthermore, the product of the gene CG3962 is the Drosophila counterpart of the mammalian Keap1, an inhibitor of Nrf2. In this study, we characterized a putative small Maf protein called Maf-S as a bona fide co-factor of CncC, thus completing the characterization of the three core components of the Nrf2 pathway in Drosophila. My studies on the CncC pathway indicate that the effective regulation of protective and antioxidant gene expression programs deteriorate in aging organisms. Whereas the steady state levels of Nrf2-regulated mRNAs do not decline with age, their inducible expression in response to acute stress exposure becomes less effective. We found evidence to suggest that the activity of the Nrf2 dimerization partner, MafS, is critical for effective induction of Nrf2 target genes. Elevated MafS levels, affected by ubiquitous transgenic over expression can restore the inducibility of antioxidant gene expression in old flies and augment their stress resistance, heart performance and general fitness but has no effect on young animals. Taken together these data suggests that MafS is an essential component of the CncC/Nrf2 pathway and is required for maintenance of the effective response of the pathway to acute stress.

Subjects/Keywords: Oxidative Stress; Oxidative Damage; Anti-Oxidant

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APA (6^th Edition):

Rahman, M. M. (2012). The Role of the Nrf2 Dimerization Partner Maf-S in Drosophila Gene Regulation, Stress Response and Aging. (Doctoral Dissertation). University of Rochester. Retrieved from http://hdl.handle.net/1802/25519

Chicago Manual of Style (16^th Edition):

Rahman, Mohammed Mahidur. “The Role of the Nrf2 Dimerization Partner Maf-S in Drosophila Gene Regulation, Stress Response and Aging.” 2012. Doctoral Dissertation, University of Rochester. Accessed March 02, 2021. http://hdl.handle.net/1802/25519.

MLA Handbook (7^th Edition):

Rahman, Mohammed Mahidur. “The Role of the Nrf2 Dimerization Partner Maf-S in Drosophila Gene Regulation, Stress Response and Aging.” 2012. Web. 02 Mar 2021.

Vancouver:

Rahman MM. The Role of the Nrf2 Dimerization Partner Maf-S in Drosophila Gene Regulation, Stress Response and Aging. [Internet] [Doctoral dissertation]. University of Rochester; 2012. [cited 2021 Mar 02]. Available from: http://hdl.handle.net/1802/25519.

Council of Science Editors:

Rahman MM. The Role of the Nrf2 Dimerization Partner Maf-S in Drosophila Gene Regulation, Stress Response and Aging. [Doctoral Dissertation]. University of Rochester; 2012. Available from: http://hdl.handle.net/1802/25519

Texas A&M University

5. Yuen, Evelyn P. Effects of High Dietary Iron and Gamma Radiation on Oxidative Stress and Bone.

Degree: MS, Nutrition, 2013, Texas A&M University

URL: http://hdl.handle.net/1969.1/149574

► Astronauts in space flight missions are exposed to increased iron (Fe) stores and galactic cosmic radiation, both of which independently induce oxidative stress. Oxidative stress… (more)

▼ Astronauts in space flight missions are exposed to increased iron (Fe) stores and galactic cosmic radiation, both of which independently induce oxidative stress. Oxidative stress can result in protein, lipid, and DNA oxidation. Recent evidence has linked oxidative stress to bone loss with aging and estrogen deficiency. Whether the increased iron stores and radiation that astronauts face are exacerbating their extreme bone loss while in space is unclear. We hypothesized that elevated iron levels (induced by feeding a high iron diet) and gamma radiation exposure would independently increase markers of oxidative stress and markers of oxidative damage and result in loss of bone mass, with the combined treatment having additive or synergistic effects. Male Sprague-Dawley rats (15-weeks old, n=32) were randomized to receive an adequate (45 mg Fe/kg diet) or high (650 mg Fe/kg diet) Fe diet for 4 weeks and either 3 Gy (8 fractions, 0.375 Gy each) of 137Cs radiation (γRAD) or sham exposure every other day over 16 days starting on day 14. Serum Fe and catalase and liver Fe and glutathione peroxidase (GPX) were assessed by standard techniques. Immunostaining for 8-hydroxy-2-deoxyguanosine (8-OHdG, marker of DNA adducts) quantified the number of cells with oxidative damage in cortical bone. Bone histomorphometry assessed bone cell activity and cancellous bone microarchitecture in the metaphyseal region. Ex vivo pQCT quantified volumetric bone mineral density (vBMD); bone mechanical strength was assessed by 3-pt bending at the midshaft tibia and compression of the femoral neck. High Fe diet increased liver Fe and decreased volume per total volume (BV/TV). γRAD decreased osteoid surface per bone surface (OS/BS) and osteocyte density. The combined treatment increased serum catalase, liver GPX, and serum iron and decreased cancellous vBMD and trabecular number (Tb.N). High Fe diet and γRAD independently increased number of osteocytes stained positive for 8-OHdG, with the combined treatment exhibiting twice as many osteocytes positively stained compared to the control. Higher serum Fe levels were associated with higher oxidative damage (r =0.38) and lower proximal tibial cancellous vBMD (r =–0.38). Higher serum catalase levels were associated with higher oxidative damage (r =0.48), lower BV/TV (r =–0.40) and lower cancellous vBMD (r =–0.39). High dietary iron and fractionated 137Cs γRAD leads to a moderate elevation in iron stores and results in oxidative damage in bone and are associated with decreased cancellous bone density. Moderate elevations in iron stores are not only found in astronauts, but also naturally occur in healthy human populations. This healthy population with elevated iron stores may also have increased levels of oxidative stress in the body. Elevated levels of oxidative stress not only increase one’s risk for accelerated bone loss, but also the risk of developing other chronic diseases such as insulin resistance, hypertension, dyslipidemia, and metabolic syndrome. Advisors/Committee Members: Bloomfield, Susan A (advisor), Turner, Nancy D (advisor), Hogan, Harry A (committee member).

Subjects/Keywords: bone; iron; radiation; oxidative stress; oxidative damage

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Yuen, E. P. (2013). Effects of High Dietary Iron and Gamma Radiation on Oxidative Stress and Bone. (Masters Thesis). Texas A&M University. Retrieved from http://hdl.handle.net/1969.1/149574

Chicago Manual of Style (16^th Edition):

Yuen, Evelyn P. “Effects of High Dietary Iron and Gamma Radiation on Oxidative Stress and Bone.” 2013. Masters Thesis, Texas A&M University. Accessed March 02, 2021. http://hdl.handle.net/1969.1/149574.

MLA Handbook (7^th Edition):

Yuen, Evelyn P. “Effects of High Dietary Iron and Gamma Radiation on Oxidative Stress and Bone.” 2013. Web. 02 Mar 2021.

Vancouver:

Yuen EP. Effects of High Dietary Iron and Gamma Radiation on Oxidative Stress and Bone. [Internet] [Masters thesis]. Texas A&M University; 2013. [cited 2021 Mar 02]. Available from: http://hdl.handle.net/1969.1/149574.

Council of Science Editors:

Yuen EP. Effects of High Dietary Iron and Gamma Radiation on Oxidative Stress and Bone. [Masters Thesis]. Texas A&M University; 2013. Available from: http://hdl.handle.net/1969.1/149574

Oregon State University

6. Wu, Jianyong. Tandem mass spectrometric analysis of protein and peptide adducts of lipid peroxidation-derived aldehydes.

Degree: PhD, Chemistry, 2010, Oregon State University

URL: http://hdl.handle.net/1957/13823

► The adduction of proteins and other biomolecules by electrophilic lipid peroxidation products such as 4-hydroxy-2-nonenal (HNE), 4-oxo-2-nonenal (ONE), malondialdehyde (MDA) or acrolein (ACR) is thought… (more)

▼ The adduction of proteins and other biomolecules by electrophilic lipid peroxidation products such as 4-hydroxy-2-nonenal (HNE), 4-oxo-2-nonenal (ONE), malondialdehyde (MDA) or acrolein (ACR) is thought to be an initiating and/or propagating factor in the pathophysiology of several diseases such as atherosclerosis, diabetes, Alzheimer's, Parkinson's and other age-related disorders. The identification of protein sites modified by oxylipids is of key relevance for advancing our understanding how oxidative damage affects structure and function of proteins. Here, the use of MALDI tandem mass spectrometry with high energy collision-induced dissociation (CID) on a TOF/TOF instrument for sequencing oxylipid-peptide conjugates was systematically studied. Three synthesized model peptides containing one nucleophilic residue (i.e. Cys, His or Lys) were reacted with MDA, HNE, ONE and ACR. MALDI-MS analysis and MS/MS analysis were performed to confirm the adduct type and the modification sites. Michael adducts and Schiff bases were the predominant products under pH 7.4 within 2 hours. All MS/MS spectra of Michael adducts show the neutral loss of the oxylipid moiety ions. MS/MS spectra of Cys-containing peptide oxylipid conjugates exhibit additional characteristic neutral loss of HS-oxylipid moiety ions. MS/MS spectra of His-containing peptide oxylipid conjugates show characteristic oxylipid-containing His immonium ions. Spectra of Lys-containing peptide oxylipid conjugates (Schiff base) also show oxylipid-containing Lys immonium ions. However, there is no neutral loss of the oxylipid moiety ion for these Schiff bases. Determining the extent or relative amounts of the oxidative damage in cells could provide valuable insights into the molecular mechanisms of the diseases caused by oxidative stress. Relative quantitation of oxylipid-modified proteins in biological samples is a challenging problem because of the complexity and extreme dynamic range that characterize these samples. In this study, the reagents, N'-aminooxymethylcarbonylhydrazino-D-biotin (ARP) and iodoacetyl-PEO2- biotin (IPB), were used to enrich acrolein-modified Cys-containing peptides and the corresponding unmodified ones from subsarcolemmal mitochondria (SSM). The ratios between them were determined by nanoLC-SRM analysis. Model Cys-containing peptides labeled with ARP-acrolein and IPB were employed to demonstrate this method. Seven acrolein-modifed Cys-containing peptides from five mitochondrial proteins were quantified. The ratios for those seven peptides from the CCl₄-treated rats are higher than the control ones indicating that the ratios of acrolein-modified peptides to unmodified ones are potential markers of oxidative stress in vivo. Age-dependent changes of protein carbonyls were investigated in subsarcolemmal mitochondria by using LC-SRM analysis of distinct ACR-modified Cys-containing peptides. Immunochemical analysis using an anti-ACR monoclonal antibody supported an increase of proteins modified by acrolein with age. However, total protein carbonyls… Advisors/Committee Members: Maier, Claudia S. (advisor), Barofsky, Doug (committee member).

Subjects/Keywords: Mass Spectrometry; Oxidative stress

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Wu, J. (2010). Tandem mass spectrometric analysis of protein and peptide adducts of lipid peroxidation-derived aldehydes. (Doctoral Dissertation). Oregon State University. Retrieved from http://hdl.handle.net/1957/13823

Chicago Manual of Style (16^th Edition):

Wu, Jianyong. “Tandem mass spectrometric analysis of protein and peptide adducts of lipid peroxidation-derived aldehydes.” 2010. Doctoral Dissertation, Oregon State University. Accessed March 02, 2021. http://hdl.handle.net/1957/13823.

MLA Handbook (7^th Edition):

Wu, Jianyong. “Tandem mass spectrometric analysis of protein and peptide adducts of lipid peroxidation-derived aldehydes.” 2010. Web. 02 Mar 2021.

Vancouver:

Wu J. Tandem mass spectrometric analysis of protein and peptide adducts of lipid peroxidation-derived aldehydes. [Internet] [Doctoral dissertation]. Oregon State University; 2010. [cited 2021 Mar 02]. Available from: http://hdl.handle.net/1957/13823.

Council of Science Editors:

Wu J. Tandem mass spectrometric analysis of protein and peptide adducts of lipid peroxidation-derived aldehydes. [Doctoral Dissertation]. Oregon State University; 2010. Available from: http://hdl.handle.net/1957/13823

Oregon State University

7. Chavez, Juan D. Mass spectrometry-based identification and characterization of protein and peptide adducts of lipoxidation-derived aldehydes.

Degree: PhD, Chemistry, 2010, Oregon State University

URL: http://hdl.handle.net/1957/13848

► Oxidative stress is recognized as an important underlying factor in the pathogenesis of many degenerative diseases as well as normal senescence. The free radicals, reactive… (more)

▼ Oxidative stress is recognized as an important underlying factor in the pathogenesis of many degenerative diseases as well as normal senescence. The free radicals, reactive oxygen species (ROS) and electrophiles produced during oxidative stress are capable of modifying nucleic acids, lipids and proteins. There are a variety of oxidative modifications that occur to proteins including: cleavage of the protein backbone, direct oxidation of amino acid side chains by ROS, and adduction by electrophilic species such as lipid peroxidation products. Many of these oxidative modifications result in the introduction of carbonyl groups into the proteins. Protein carbonylation levels are commonly used as a biomarker to assess the degree of oxidative damage to a system. However the most commonly employed methods for measuring oxidative modifications to proteins, typically fail to provide any information about the identity of the modified protein, site of modification, or the chemical nature of the modification. In the present study we develop an analytical technique based on affinity labeling with N'-aminooxymethylcarbonylhydrazino-D-biotin (aldehyde reactive probe, ARP), along with mass spectrometric analysis which allows for the full characterization of protein carbonylation modifications. The ability of the ARP method was first demonstrated for the case of oxylipid peptide and protein conjugates formed by Michael addition-type conjugation reactions with α,β- unsaturated aldehydic lipid peroxidation products with nucleophilic amino acid residue side chains. ARP was used to label a 4-hydroxy-2-nonenal (HNE) modified cysteine containing model peptide, and HNE modified E. coli thioredoxin, which were characterized using ESI-MS/MS and MALDI-MS/MS. ARP was also used to label the oxidative modifications alpha-aminoadipic semialdehyde (AAS) and gamma-glutamic semialdehyde (GGS), formed during the metal catalyzed oxidation of GAPDH. After demonstrating the utility of the technique on model systems, it was then applied to complex biological systems. In one case, subsarcolemmal mitochondria (SSM) isolated from rat cardiac tissue. Mitochondria are well known to be a major source of ROS within the cell. They are therefore important mediators of oxidative stress, as well as regulators of cell death. We were able to identify 39 unique sites on 27 mitochondrial proteins which were modified by six different α,β-unsaturated aldehydes, including acrolein, β-hydroxyacrolein, crotonaldehyde, 4-hydroxy-2-hexenal, 4-hydroxy-2-nonenal and 4-oxo-2- nonenal. Additionally we identified nine Lys residues on four mitochondrial proteins that were oxidized to AAS and subsequently labeled with ARP. The proteins identified with oxidative modifications include members of the mitochondrial electron transport chain, TCA cycle, membrane transport, lipid metabolism, and other important mitochondrial enzymes. The ARP technique was also applied to identify… Advisors/Committee Members: Maier, Claudia S. (advisor), Barofsky, Doug (committee member).

Subjects/Keywords: mass spectrometry; Oxidative stress

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APA (6^th Edition):

Chavez, J. D. (2010). Mass spectrometry-based identification and characterization of protein and peptide adducts of lipoxidation-derived aldehydes. (Doctoral Dissertation). Oregon State University. Retrieved from http://hdl.handle.net/1957/13848

Chicago Manual of Style (16^th Edition):

Chavez, Juan D. “Mass spectrometry-based identification and characterization of protein and peptide adducts of lipoxidation-derived aldehydes.” 2010. Doctoral Dissertation, Oregon State University. Accessed March 02, 2021. http://hdl.handle.net/1957/13848.

MLA Handbook (7^th Edition):

Chavez, Juan D. “Mass spectrometry-based identification and characterization of protein and peptide adducts of lipoxidation-derived aldehydes.” 2010. Web. 02 Mar 2021.

Vancouver:

Chavez JD. Mass spectrometry-based identification and characterization of protein and peptide adducts of lipoxidation-derived aldehydes. [Internet] [Doctoral dissertation]. Oregon State University; 2010. [cited 2021 Mar 02]. Available from: http://hdl.handle.net/1957/13848.

Council of Science Editors:

Chavez JD. Mass spectrometry-based identification and characterization of protein and peptide adducts of lipoxidation-derived aldehydes. [Doctoral Dissertation]. Oregon State University; 2010. Available from: http://hdl.handle.net/1957/13848

Universiteit Utrecht

8. Vorage, M.S.C.E. What are the roles of oxidative stress and pro-inflammatory cytokines in fibromyalgia?.

Degree: 2011, Universiteit Utrecht

URL: http://dspace.library.uu.nl:8080/handle/1874/213023

► Fibromyalgia (FM) is a common disorder of unclear aetiology, characterized by chronic widespread pain and painful tender points (body pressure points). Several researchers suggest that… (more)

Subjects/Keywords: Fibromyalgia; cytokines; oxidative stress

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APA (6^th Edition):

Vorage, M. S. C. E. (2011). What are the roles of oxidative stress and pro-inflammatory cytokines in fibromyalgia?. (Masters Thesis). Universiteit Utrecht. Retrieved from http://dspace.library.uu.nl:8080/handle/1874/213023

Chicago Manual of Style (16^th Edition):

Vorage, M S C E. “What are the roles of oxidative stress and pro-inflammatory cytokines in fibromyalgia?.” 2011. Masters Thesis, Universiteit Utrecht. Accessed March 02, 2021. http://dspace.library.uu.nl:8080/handle/1874/213023.

MLA Handbook (7^th Edition):

Vorage, M S C E. “What are the roles of oxidative stress and pro-inflammatory cytokines in fibromyalgia?.” 2011. Web. 02 Mar 2021.

Vancouver:

Vorage MSCE. What are the roles of oxidative stress and pro-inflammatory cytokines in fibromyalgia?. [Internet] [Masters thesis]. Universiteit Utrecht; 2011. [cited 2021 Mar 02]. Available from: http://dspace.library.uu.nl:8080/handle/1874/213023.

Council of Science Editors:

Vorage MSCE. What are the roles of oxidative stress and pro-inflammatory cytokines in fibromyalgia?. [Masters Thesis]. Universiteit Utrecht; 2011. Available from: http://dspace.library.uu.nl:8080/handle/1874/213023

Mississippi State University

9. Basham, Steven Allen. Effect of curcumin supplementation on exercise-induced oxidative stress, inflammation, and muscle damage.

Degree: MS, Kinesiology, 2018, Mississippi State University

URL: http://sun.library.msstate.edu/ETD-db/theses/available/etd-03212018-144057/ ;

► Oxidative stress (OS) and inflammation can be detrimental to exercise performance. Antioxidants such as curcumin are shown to reduce exercise-induced OS, inflammation, muscle damage,… (more)

Subjects/Keywords: Oxidative Stress; Curcumin; Inflammation

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APA (6^th Edition):

Basham, S. A. (2018). Effect of curcumin supplementation on exercise-induced oxidative stress, inflammation, and muscle damage. (Masters Thesis). Mississippi State University. Retrieved from http://sun.library.msstate.edu/ETD-db/theses/available/etd-03212018-144057/ ;

Chicago Manual of Style (16^th Edition):

Basham, Steven Allen. “Effect of curcumin supplementation on exercise-induced oxidative stress, inflammation, and muscle damage.” 2018. Masters Thesis, Mississippi State University. Accessed March 02, 2021. http://sun.library.msstate.edu/ETD-db/theses/available/etd-03212018-144057/ ;.

MLA Handbook (7^th Edition):

Basham, Steven Allen. “Effect of curcumin supplementation on exercise-induced oxidative stress, inflammation, and muscle damage.” 2018. Web. 02 Mar 2021.

Vancouver:

Basham SA. Effect of curcumin supplementation on exercise-induced oxidative stress, inflammation, and muscle damage. [Internet] [Masters thesis]. Mississippi State University; 2018. [cited 2021 Mar 02]. Available from: http://sun.library.msstate.edu/ETD-db/theses/available/etd-03212018-144057/ ;.

Council of Science Editors:

Basham SA. Effect of curcumin supplementation on exercise-induced oxidative stress, inflammation, and muscle damage. [Masters Thesis]. Mississippi State University; 2018. Available from: http://sun.library.msstate.edu/ETD-db/theses/available/etd-03212018-144057/ ;

Vanderbilt University

10. Ni, Mingwei. Acute response of primary glial cells to methylmercury exposure.

Degree: PhD, Pharmacology, 2011, Vanderbilt University

URL: http://hdl.handle.net/1803/14320

► Mercury accumulates in fish-eating populations. Glial cells have diverse functions including providing nutrition[1], maintaining CNS homeostasis, removing pathogens, inducing neuronal differentiation and mediating CNS immune… (more)

▼ Mercury accumulates in fish-eating populations. Glial cells have diverse functions including providing nutrition[1], maintaining CNS homeostasis, removing pathogens, inducing neuronal differentiation and mediating CNS immune responsiveness. Dysfunction of glial cells is contributed to MeHg-induced brain damage. We hypothesized that 1) the cellular responses to MeHg are cell-type specific; 2) differences exist in the uptake of MeHg between astrocytes and microglia, leading to differential temporal cellular responses. I tested the unique response of primary glial cells to MeHg exposure at physiologically relevant concentration. The results are presented in this dissertation. Firstly, I established the methodology to separate a large amount of rat primary microglial cells with high purity (>95%) from mixed glial cell culture. Secondly, I investigated the toxic effects of MeHg on primary glial cells as well as their cellular response to acute MeHg exposure at environmentally relevant concentrations (0.1µM~ 5µM). The results showed MeHg treatment caused a concentration- and time- dependent microglial cell death, intracellular ROS generation and GSH depletion. I analyzed the dynamic changes of nuclear factor erythroid- derived 2- like 2 (NFE2L2) in both cytosolic fraction and nuclear fraction. My results suggested that the increase in Nrf2 protein level and the subsequent nuclear translocation are regulated by ROS in both glial cell types. However, Nrf2 changes in astrocytes occurred on a protracted time scale. Thirdly, the effects of Nrf2 on its downstream gene expression and cell viability post MeHg exposure were further studied, using short hairpin RNA (shRNA) approach. Finally the responses of rat primary microglial cells post MeHg treatment were compared with those of rat primary astrocytes. In conclusion, our study has demonstrated that microglial cells are more sensitive than astrocytes to MeHg. Their regulation kinetics differ, therefore allowing astrocytes and microglial cells to play different roles in mediating MeHg toxicity. Advisors/Committee Members: Eugenia Gurevich (committee member), Michael Aschner (committee member), Ronald Wiley (committee member), Aaron Bowman (committee member), Ariel Deutch (Committee Chair).

Subjects/Keywords: methylmercury; Nrf2; oxidative stress; glia

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APA (6^th Edition):

Ni, M. (2011). Acute response of primary glial cells to methylmercury exposure. (Doctoral Dissertation). Vanderbilt University. Retrieved from http://hdl.handle.net/1803/14320

Chicago Manual of Style (16^th Edition):

Ni, Mingwei. “Acute response of primary glial cells to methylmercury exposure.” 2011. Doctoral Dissertation, Vanderbilt University. Accessed March 02, 2021. http://hdl.handle.net/1803/14320.

MLA Handbook (7^th Edition):

Ni, Mingwei. “Acute response of primary glial cells to methylmercury exposure.” 2011. Web. 02 Mar 2021.

Vancouver:

Ni M. Acute response of primary glial cells to methylmercury exposure. [Internet] [Doctoral dissertation]. Vanderbilt University; 2011. [cited 2021 Mar 02]. Available from: http://hdl.handle.net/1803/14320.

Council of Science Editors:

Ni M. Acute response of primary glial cells to methylmercury exposure. [Doctoral Dissertation]. Vanderbilt University; 2011. Available from: http://hdl.handle.net/1803/14320

11. Asrar Ahmad. Biochemical studies on markers of oxidative stress and antioxidants in patients with obstructive airway diseases; -.

Degree: Biochemistry, 2012, Aligarh Muslim University

URL: http://shodhganga.inflibnet.ac.in/handle/10603/11336

None

Summary p.111-114, Bibliography p.115-150, List of publications and presentations p.151-153, Appendices p.154-156

Advisors/Committee Members: Qayyum Husain.

Subjects/Keywords: biochemistry; oxidative stress; asthma

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APA (6^th Edition):

Ahmad, A. (2012). Biochemical studies on markers of oxidative stress and antioxidants in patients with obstructive airway diseases; -. (Thesis). Aligarh Muslim University. Retrieved from http://shodhganga.inflibnet.ac.in/handle/10603/11336

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Ahmad, Asrar. “Biochemical studies on markers of oxidative stress and antioxidants in patients with obstructive airway diseases; -.” 2012. Thesis, Aligarh Muslim University. Accessed March 02, 2021. http://shodhganga.inflibnet.ac.in/handle/10603/11336.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Ahmad, Asrar. “Biochemical studies on markers of oxidative stress and antioxidants in patients with obstructive airway diseases; -.” 2012. Web. 02 Mar 2021.

Vancouver:

Ahmad A. Biochemical studies on markers of oxidative stress and antioxidants in patients with obstructive airway diseases; -. [Internet] [Thesis]. Aligarh Muslim University; 2012. [cited 2021 Mar 02]. Available from: http://shodhganga.inflibnet.ac.in/handle/10603/11336.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Ahmad A. Biochemical studies on markers of oxidative stress and antioxidants in patients with obstructive airway diseases; -. [Thesis]. Aligarh Muslim University; 2012. Available from: http://shodhganga.inflibnet.ac.in/handle/10603/11336

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Saskatchewan

12. Peng, Fei. Mitochondrial uptake of anthocyanidins and protection from oxidative stress.

Degree: 2012, University of Saskatchewan

URL: http://hdl.handle.net/10388/ETD-2012-08-626

► The anthocyanins show efficient antioxidant properties and free radical scavenging properties which result in various health-promoting benefits. This research investigated the ability of anthocyanidins to… (more)

▼ The anthocyanins show efficient antioxidant properties and free radical scavenging properties which result in various health-promoting benefits. This research investigated the ability of anthocyanidins to distribute into mitochondria and protect mitochondria from oxidative stress. In an in vitro study, the uptake of pure cyanidin and quercetin, and their 3-glucosylated forms into isolated rat liver mitochondria was tested, along with their effects on mitochondrial oxidative stress parameters. The absorption of cyanidin was significantly higher (67% uptake of 125 µM) than the other three flavonoids. Measurements indicated that the cyanidin was taken up into or tightly bound by mitochondria. Also, results suggested that cyanidin uptake was partially dependent on membrane potential. When incubated together (internally and externally) with mitochondria all tested flavonoids decreased reactive oxygen species (ROS) generation during mitochondrial respiration, and inhibited lipid peroxidation to different extents. Importantly, pre-loaded CY showed much stronger effects against oxidative stress in two analyses than other flavonoids. Due to its greater uptake by mitochondria, cyanidin may provide greater protection in vivo. In an in vivo study, cyanidin, quercetin and their 3-glucosides were administered into rat tail vein to give a dose of 7.6 µmol/Kg body weight. Cyanidin and its glucoside had greater affinity to liver and kidney than did quercetin and its glucoside; particularly, all test tissues contained a significantly higher amount of cyanidin than other test flavonoids. Also, cyanidin accumulated more in liver mitochondria than other flavonoids, and consistent with in vitro results was present in mitochondria to a much greater extent than cyanidin glucoside. However, delivery of the flavonoids at this dose did not significantly affect the liver mitochondria susceptibility to lipid peroxidation or the level of endogenous tissue oxidative damage. Altogether the results show that cyanidin can rapidly and efficiently accumulate in mitochondria, wherein it exhibits strong bio-antioxidant activity against oxidative stress and may help protect mitochondrial function and integrity. Also, the anthocyanidin and its 3-glucoside have greater ability than flavonols to accumulate in organs; especially cyanidin presented in liver mitochondria to a much greater extent. Cyanidin could be a potent natural antioxidant compound that is effective in mitochondria-protective therapies. Advisors/Committee Members: Bandy, Brian, Alcorn, Jane, Low, Nicholas.

Subjects/Keywords: Anthocyanidins; Oxidative stress; Antioxidant; Mitochondria

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APA (6^th Edition):

Peng, F. (2012). Mitochondrial uptake of anthocyanidins and protection from oxidative stress. (Thesis). University of Saskatchewan. Retrieved from http://hdl.handle.net/10388/ETD-2012-08-626

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Peng, Fei. “Mitochondrial uptake of anthocyanidins and protection from oxidative stress.” 2012. Thesis, University of Saskatchewan. Accessed March 02, 2021. http://hdl.handle.net/10388/ETD-2012-08-626.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Peng, Fei. “Mitochondrial uptake of anthocyanidins and protection from oxidative stress.” 2012. Web. 02 Mar 2021.

Vancouver:

Peng F. Mitochondrial uptake of anthocyanidins and protection from oxidative stress. [Internet] [Thesis]. University of Saskatchewan; 2012. [cited 2021 Mar 02]. Available from: http://hdl.handle.net/10388/ETD-2012-08-626.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Peng F. Mitochondrial uptake of anthocyanidins and protection from oxidative stress. [Thesis]. University of Saskatchewan; 2012. Available from: http://hdl.handle.net/10388/ETD-2012-08-626

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Vilnius University

13. Stražnickienė, Alina. Flavonoidų poveikis Hep 22a linijoms ląstelėms.

Degree: Master, 2014, Vilnius University

URL: http://vddb.laba.lt/obj/LT-eLABa-0001:E.02~2006~D_20140702_190409-12658 ;

►

Ląstelės metabolizme svarbų vaidmenį vaidina oksidacijos – redukcijos reakcijos, kuriose deguonis yra elektronų akceptorius. Įvairių cheminių reakcijų metu gali susidaryti ir aktyviosios deguonies formos –… (more)

▼

Ląstelės metabolizme svarbų vaidmenį vaidina oksidacijos – redukcijos reakcijos, kuriose deguonis yra elektronų akceptorius. Įvairių cheminių reakcijų metu gali susidaryti ir aktyviosios deguonies formos – superoksido anijonas, vandenilio peroksidas, hidroksilo radikalas, singletinis deguonis, kurios pažeidžia įvairias biomolekules. Augalai nuo seniausių laikų yra vartojami įvairioms ligoms gydyti. Flavonoidai – tai augalinės kilmės junginiai, randami vaisiuose, daržovėse arbatose, kurie pasižymi antioksidacinėmis savybėmis. Sintetiniai antioksidantai polifenoliai labai plačiai naudojami maisto pramonėje, kaip įvairūs priedai ir konservantai. Vienas jų - kvercetinas. Kvercetino antioksidacinis poveikis priklauso nuo to, kad jis reaguoja su laisvaisiais radikalais, sudarydamas fenoksradikalus, kurie yra ne tokie aktyvūs. Tačiau aukštos kvercetino koncentracijos yra citotoksiškos, o citotoksiškumo mechanizmai, nors ir labai plačiai tyrinėjami visame pasaulyje, lieka neaiškūs. Mūsų darbo tikslas ir buvo ištirti kvercetino ir kitų flavonoidų poveikį Hep 22a linijos ląstelėms. Hep 22a ląstelių linija pasirinkta neatsitiktinai. Tai pelių hepatomos ląstelių kultūra, kuri pasižymi navikinėms ląstelėms būdingomis savybėmis – neribota proliferacija ir ląstelių migracija. Vyrauja epitelinio tipo ląstelės, kurias persėjus po oda susidaro navikai. Ląstelės gerai auga tiek in vivo, tiek in vitro. Ląstelės pailgos, prisitvirtinusios prie substrato, sudaro monosluoksnį. Tirtų flavonoidų... [toliau žr. visą tekstą]

ABSTRACT Flavonoids are widely distributed in edible plants, and considered to be dietary antioxidants. Flavonoids can protect cell from „oxidative stress“, but the same flavonoids compound could behave in two ways as an both antioxidant and prooxidant, depending on the concentration used and free radical source. Among the flavonoids, quercetin is one of the most widely studied flavonoid and has biological, pharmocological, and medicinal properties. Besides the chemopreventive effects, other biological functions of quercetin are believed to improve antioxidant defence systems in living organizms. The aim of this work was to analize the effects of flavonoids (quercetin, myricetin and morin) in Hep 22a cells. Materials and methods: 1. Cell culture cytotoxicity studies; Flavonoids and the other components were obtained from Sigma, and used as received. 2. Study with fluorescence microscope 3. Statistical analysis Results and discusion: Hep 22a cell line is a mouse hepatoma cell line, which posseses the unlimited proliferation and migration features. Quercetin concentration for 50 % death of Hep 22a cells (cL50) was 160 µM, myricetin concentration was 60 µM, and morin concentration was 190 µM,. The citotoxity of flavonoids in Hep 22a cells was partly inhibited by catalase, by the antioxidant N,N‘-diphenyl-p-phenylene diamine DPPD, desferrioxamine and by dicumarol and an inhibitor of DT-diaphorase thus showing its prooxidant character. Inhibitors of cytochromes P-450, α... [to full text]

Advisors/Committee Members: Nemeikaitė-Čėnienė, Aušra (Master's thesis supervisor).

Subjects/Keywords: Oxidative stress; Flavonoids; quercetin; Apoptosis

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APA (6^th Edition):

Stražnickienė, Alina. (2014). Flavonoidų poveikis Hep 22a linijoms ląstelėms. (Masters Thesis). Vilnius University. Retrieved from http://vddb.laba.lt/obj/LT-eLABa-0001:E.02~2006~D_20140702_190409-12658 ;

Note: this citation may be lacking information needed for this citation format:
Author name may be incomplete

Chicago Manual of Style (16^th Edition):

Stražnickienė, Alina. “Flavonoidų poveikis Hep 22a linijoms ląstelėms.” 2014. Masters Thesis, Vilnius University. Accessed March 02, 2021. http://vddb.laba.lt/obj/LT-eLABa-0001:E.02~2006~D_20140702_190409-12658 ;.

Note: this citation may be lacking information needed for this citation format:
Author name may be incomplete

MLA Handbook (7^th Edition):

Stražnickienė, Alina. “Flavonoidų poveikis Hep 22a linijoms ląstelėms.” 2014. Web. 02 Mar 2021.

Note: this citation may be lacking information needed for this citation format:
Author name may be incomplete

Vancouver:

Stražnickienė, Alina. Flavonoidų poveikis Hep 22a linijoms ląstelėms. [Internet] [Masters thesis]. Vilnius University; 2014. [cited 2021 Mar 02]. Available from: http://vddb.laba.lt/obj/LT-eLABa-0001:E.02~2006~D_20140702_190409-12658 ;.

Note: this citation may be lacking information needed for this citation format:
Author name may be incomplete

Council of Science Editors:

Stražnickienė, Alina. Flavonoidų poveikis Hep 22a linijoms ląstelėms. [Masters Thesis]. Vilnius University; 2014. Available from: http://vddb.laba.lt/obj/LT-eLABa-0001:E.02~2006~D_20140702_190409-12658 ;

Note: this citation may be lacking information needed for this citation format:
Author name may be incomplete

University of Connecticut

14. DiMarco, Diana M. Reduction of Obesity-Associated Oxidative Stress by Low-Fat Yogurt Consumption.

Degree: MS, Nutritional Science, 2014, University of Connecticut

URL: https://opencommons.uconn.edu/gs_theses/668

► Excess macronutrient intake leads to an increase in formation of reactive oxygen species (ROS). This causes an imbalance between ROS generation and the ability… (more)

▼ Excess macronutrient intake leads to an increase in formation of reactive oxygen species (ROS). This causes an imbalance between ROS generation and the ability of the body to neutralize these radicals, a state known as oxidative stress. Previous research suggests the ability of dairy products to attenuate the postprandial response, which may reduce ROS formation. Therefore, this study aimed to elucidate the impact of low-fat yogurt consumption on fasting and postprandial oxidative stress in obese women. We hypothesized that co-consumption of low-fat yogurt and a high-calorie, high-fat meal would reduce postprandial elevations in oxidative stress in obese women. To test this hypothesis, healthy, lean and obese women co-consumed low-fat yogurt or soy pudding (control) and a high-calorie, high-fat meal on 2 occasions separated by 9-week daily yogurt or pudding consumption. Postprandial blood samples were collected for 4 h during meal challenges and fasting samples were collected at 3 week intervals throughout the duration of the intervention. Fasting and postprandial plasma samples were analyzed for malondialdehyde (MDA), total thiols (SH), and advanced glycation endproducts (AGEs). A secondary aim of this work was to classify changes in dietary intake associated with the intervention; data was based on 3-day self-reported dietary records. Low-fat yogurt consumption did not lead to changes in BMI, waist circumference, or blood pressure. Consumption of low-fat yogurt attenuated postprandial increases in MDA in obese women at both the initial and final meal challenges. However, 9-week yogurt consumption did not further attenuate the postprandial response and had no effect on fasting MDA concentrations in obese women. Yogurt and pudding prevented postprandial changes in SH and AGEs, while chronic plasma SH and AGEs were unaffected by 9-week yogurt or soy pudding intake. Daily consumption of low-fat yogurt and soy pudding increased calcium and vitamin D intake and yogurt increased dairy intake in lean and obese women. Therefore, the addition of low-fat yogurt to the diet may be a strategy to increase dairy, calcium, and vitamin D intake without inducing any unfavorable changes in body composition, blood pressure, and postprandial or chronic levels of oxidative stress. Advisors/Committee Members: Dr. Ock Chun and Dr. Maria-Luz Fernandez, Dr. Bradley Bolling.

Subjects/Keywords: Obesity; Yogurt; Dairy; Oxidative Stress

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APA (6^th Edition):

DiMarco, D. M. (2014). Reduction of Obesity-Associated Oxidative Stress by Low-Fat Yogurt Consumption. (Masters Thesis). University of Connecticut. Retrieved from https://opencommons.uconn.edu/gs_theses/668

Chicago Manual of Style (16^th Edition):

DiMarco, Diana M. “Reduction of Obesity-Associated Oxidative Stress by Low-Fat Yogurt Consumption.” 2014. Masters Thesis, University of Connecticut. Accessed March 02, 2021. https://opencommons.uconn.edu/gs_theses/668.

MLA Handbook (7^th Edition):

DiMarco, Diana M. “Reduction of Obesity-Associated Oxidative Stress by Low-Fat Yogurt Consumption.” 2014. Web. 02 Mar 2021.

Vancouver:

DiMarco DM. Reduction of Obesity-Associated Oxidative Stress by Low-Fat Yogurt Consumption. [Internet] [Masters thesis]. University of Connecticut; 2014. [cited 2021 Mar 02]. Available from: https://opencommons.uconn.edu/gs_theses/668.

Council of Science Editors:

DiMarco DM. Reduction of Obesity-Associated Oxidative Stress by Low-Fat Yogurt Consumption. [Masters Thesis]. University of Connecticut; 2014. Available from: https://opencommons.uconn.edu/gs_theses/668

Colorado State University

15. Bruns, Danielle Reuland. Oxidative and energetic stress: regulation of Nrf2 and mitochondrial biogenesis for slowed aging interventions.

Degree: PhD, Health and Exercise Science, 2013, Colorado State University

URL: http://hdl.handle.net/10217/80934

► The following dissertation describes a series of experiments with the overall aim to understand the cellular energetic and oxidative stresses associated with aging, and to… (more)

▼ The following dissertation describes a series of experiments with the overall aim to understand the cellular energetic and oxidative stresses associated with aging, and to investigate treatments which may attenuate these stresses and promote healthspan. The specific aims of the four sets of experiments were 1) to determine if treatment with the phytochemicals in Protandim activates Nrf2 and 2) the mechanisms by which this activation occurs; 3) to assess sexually dimorphic Nrf2 signaling across three rodent models of longevity; and 4) to determine if mitochondrial related proteins are preferentially translated under energetic stress. In Experiments1 and #2, we found that phytochemicals activate Nrf2 and protect cells against oxidant stress. None of the mechanisms we investigated appear to be responsible for phytochemical-induced Nrf2 activation, and continued investigations must be undertaken to identify how Protandim robustly induces Nrf2 nuclear accumulation. In Experiment #3, we found that Nrf2 signaling was not consistently upregulated in tissues from long-lived models compared to controls, but we did elucidate important sex differences, with female mice generally displaying greater Nrf2 signaling than male mice. We believe this finding, in the context of sexual dimorphism in aging, warrants future investigations into Nrf2, stress resistance, and longevity between males and females. In Experiment #4, we found that mitochondrial proteins were preferentially translated upon pharmaceutical energetic stress, and that this selective translation occurred in the vicinity of the mitochondria. Our results indicate activation of Nrf2 protects cells against oxidant stress, and may be a therapeutic target for cardiovascular diseases and other age-related diseases. Further, we assess selective translation of mitochondrial proteins during energetic stress as a means of understanding how energetic stress mimetics selectively facilitate the translation of key mitochondrial proteins. Taken together, these studies provide the basis for future work aimed at attenuating diseases with oxidant stress and mitochondrial dysfunction components. Advisors/Committee Members: Hamilton, Karyn L. (advisor), Miller, Benjamin F. (advisor), Wilusz, Carol J. (committee member), Pagliassotti, Michael J. (committee member).

Subjects/Keywords: aging; oxidative stress; Nrf2; mitochondria

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APA (6^th Edition):

Bruns, D. R. (2013). Oxidative and energetic stress: regulation of Nrf2 and mitochondrial biogenesis for slowed aging interventions. (Doctoral Dissertation). Colorado State University. Retrieved from http://hdl.handle.net/10217/80934

Chicago Manual of Style (16^th Edition):

Bruns, Danielle Reuland. “Oxidative and energetic stress: regulation of Nrf2 and mitochondrial biogenesis for slowed aging interventions.” 2013. Doctoral Dissertation, Colorado State University. Accessed March 02, 2021. http://hdl.handle.net/10217/80934.

MLA Handbook (7^th Edition):

Bruns, Danielle Reuland. “Oxidative and energetic stress: regulation of Nrf2 and mitochondrial biogenesis for slowed aging interventions.” 2013. Web. 02 Mar 2021.

Vancouver:

Bruns DR. Oxidative and energetic stress: regulation of Nrf2 and mitochondrial biogenesis for slowed aging interventions. [Internet] [Doctoral dissertation]. Colorado State University; 2013. [cited 2021 Mar 02]. Available from: http://hdl.handle.net/10217/80934.

Council of Science Editors:

Bruns DR. Oxidative and energetic stress: regulation of Nrf2 and mitochondrial biogenesis for slowed aging interventions. [Doctoral Dissertation]. Colorado State University; 2013. Available from: http://hdl.handle.net/10217/80934

Colorado State University

16. Merriman, Leslie A. Relationship quality and men's oxidative stress.

Degree: MS(M.S.), Psychology, 2013, Colorado State University

URL: http://hdl.handle.net/10217/81045

► The association between oxidative stress and quality of romantic relationships was investigated in a sample of 98 college males. Given a postulated life history trade-off… (more)

Subjects/Keywords: men's health; relationships; oxidative stress

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APA (6^th Edition):

Merriman, L. A. (2013). Relationship quality and men's oxidative stress. (Masters Thesis). Colorado State University. Retrieved from http://hdl.handle.net/10217/81045

Chicago Manual of Style (16^th Edition):

Merriman, Leslie A. “Relationship quality and men's oxidative stress.” 2013. Masters Thesis, Colorado State University. Accessed March 02, 2021. http://hdl.handle.net/10217/81045.

MLA Handbook (7^th Edition):

Merriman, Leslie A. “Relationship quality and men's oxidative stress.” 2013. Web. 02 Mar 2021.

Vancouver:

Merriman LA. Relationship quality and men's oxidative stress. [Internet] [Masters thesis]. Colorado State University; 2013. [cited 2021 Mar 02]. Available from: http://hdl.handle.net/10217/81045.

Council of Science Editors:

Merriman LA. Relationship quality and men's oxidative stress. [Masters Thesis]. Colorado State University; 2013. Available from: http://hdl.handle.net/10217/81045

University of Adelaide

17. Moussavi Nik, Seyyed Hani. Molecular responses to low oxygen levels/oxidative stress in zebrafish.

Degree: 2011, University of Adelaide

URL: http://hdl.handle.net/2440/72269

► Alzheimer’s disease (AD) is a progressive neurodegenerative disorder with pathologies such as neuron loss, glial cell proliferation, extracellular deposition of senile plaques from the accumulation… (more)

▼ Alzheimer’s disease (AD) is a progressive neurodegenerative disorder with pathologies such as neuron loss, glial cell proliferation, extracellular deposition of senile plaques from the accumulation of amyloid beta (Aβ) peptides and deposition of intracellular neurofibrillary tangles. Aβ is created from the cleavage of the Amyloid Precursor Protein (APP) by two different types of aspartyl proteases, β- and γ-secretase. The majority of AD cases are sporadic and have a late onset.Mutations in the genes encoding APP, PRESENILIN1 and 2 (PSEN1 and PSEN2) genes cause an autosomal dominant inherited form of the disease with an early onset known as familial AD. In some sporadic cases an aberrant splice variant of PSEN2named PS2V is formed that can be found in inclusion bodies in the brain. PS2V results from the binding of the High Mobility Group A1a (HMGA1a) protein close to the splice donor site of exon 5 of PSEN2. The High Mobility Group A1 protein,HMGA1, is widely expressed during embryo development but not in adults. Its expression can be induced in adult neurons by hypoxia/oxidative stress and it is commonly reactivated in many types of cancer. Hypoxia can be a direct consequence of hypoperfusion, a common vascular component among Alzheimer’s disease risk factors and may play an important role in AD pathogenesis. BETA-SITE AMYLOID BETA A4 PRECURSOR PROTEIN-CLEAVING ENZYME 1, BACE1 is responsible, with γ-secretase, for cleavage of AMYLOID PRECURSOR PROTEIN, APP to produce Aβ peptide. A recent study observed that oxidative stress upregulates BACE1 expression via a regulatory pathway that is dependent on γ-secretase cleavage of APP and that results in increased Aβ peptide production. In this thesis, we define strategies for exposure of zebrafish to hypoxia and “chemical hypoxia”. We identifiyendogenous zebrafish hmga1 in an attempt to investigate PS2V formation in fish. We also demonstrate that responses to low oxygen/oxidative stress by genes involved in Alzheimer’s disease are evolutionarily conserved in fish. Paper1 (thesis chapter in the form of a manuscript) describes the identification of the hmga1 gene in zebrafish which is an orthologue of human HMGA1. It also examines the regulation of this gene under hypoxia/oxidative stress conditions and demonstrates thathmga1 expression is induced under these conditions. However no PS2V-like splice variant of zebrafish psen2 is observed. Paper 2 (thesis chapter in the form of a manuscript) describes the identification of the zebrafish bace1 gene which is orthologous to human BACE1. It also examines the regulation of AD-related genes under hypoxia/oxidative stress. We show that the response of the BACE1-PSEN-APPregulatory axisto hypoxia/oxidative stress is evolutionarily conserved between fish and mammals. Therefore, we also demonstrate that zebrafish are a valid model system for analysis of the effects of hypoxia/oxidative stress on genes associated with Alzheimer’s disease. Advisors/Committee Members: Lardelli, Michael T. (advisor), School of Molecular and Biomedical Science (school).

Subjects/Keywords: hypoxia; zebrafish; oxidative stress

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Moussavi Nik, S. H. (2011). Molecular responses to low oxygen levels/oxidative stress in zebrafish. (Thesis). University of Adelaide. Retrieved from http://hdl.handle.net/2440/72269

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Moussavi Nik, Seyyed Hani. “Molecular responses to low oxygen levels/oxidative stress in zebrafish.” 2011. Thesis, University of Adelaide. Accessed March 02, 2021. http://hdl.handle.net/2440/72269.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Moussavi Nik, Seyyed Hani. “Molecular responses to low oxygen levels/oxidative stress in zebrafish.” 2011. Web. 02 Mar 2021.

Vancouver:

Moussavi Nik SH. Molecular responses to low oxygen levels/oxidative stress in zebrafish. [Internet] [Thesis]. University of Adelaide; 2011. [cited 2021 Mar 02]. Available from: http://hdl.handle.net/2440/72269.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Moussavi Nik SH. Molecular responses to low oxygen levels/oxidative stress in zebrafish. [Thesis]. University of Adelaide; 2011. Available from: http://hdl.handle.net/2440/72269

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Adelaide

18. Oliver-Baxter, Jodie Merle. The psychoneuroimmunology of women experiencing stressful life events: testing the oxidative model.

Degree: 2011, University of Adelaide

URL: http://hdl.handle.net/2440/74664

► It has been acknowledged that psychological stress can impact on one’s health. A definitive link between psychological state, immune suppression, and disease has yet to… (more)

▼ It has been acknowledged that psychological stress can impact on one’s health. A definitive link between psychological state, immune suppression, and disease has yet to be established. A possible mechanism has been termed The Oxidative Model. This refers to the oxidative imbalance of cells associated with antioxidant status and psychological distress. The aim of this dissertation was to use this theoretical model to establish an evidence basis for future interventions in vulnerable populations. For cancer patients the post-treatment period has been identified as psychologically challenging. In addition bio-psycho-immunological models remain underexplored in post-treatment breast cancer samples to date. Two longitudinal studies were employed. The first, an observational study of a sample of women (N=17) concluding treatment (chemotherapy and/or radiotherapy) for early stage (I-III) breast cancer at the Royal Adelaide Hospital, South Australia. The second study tested the benefits of antioxidants during prolonged stress using an 8-week RCT. A sample of general population women (N=60) reporting mild to severe psychological distress was recruited. Psychological parameters measured included Psychological Distress, Defense Styles, Loneliness, Anger Expression, Psychological Adjustment, the Impact of Events Scale (IES-R), and State-Trait Depression, Curiosity, Anxiety, and Anger. Biochemical parameters included 5’-ectonucleotidase (NT), homocysteine (HCY), tissue ascorbate (VIT C), c-reactive protein (CRP), cholesterol (CHOL), folate (FOLATE), Vitamin B12 (VIT B12), and inflammatory cytokines (IL-1 β, IL-5, IL-6, IFN-γ, TNF-α, TNF-β, and IL-10). Findings from study 1 indicated severe psychological distress was experienced for a subset of breast cancer patients post-treatment. Fluctuating levels of psychological distress, anger, anxiety, and curiosity were observed across the 20-weeks. A pro-oxidant state was evident during this period. Pro-inflammatory measures were low and relatively stable. Associations between psychological measures and biomarkers supported Oxidative Model relationships. The second study revealed improved pro-oxidant and pro-inflammatory biomarkers favoured the multivitamin supplemented group. Collectively both studies reveal the influence of demographic and health behaviours on bio-psycho-social measures central to the Oxidative Model propositions. This thesis brings out the case for exploring complementary interventions, like multivitamin use, in the post-treatment period for those patients experiencing distress. Advisors/Committee Members: Turnbull, Deborah Anne (advisor), Olver, Ian N. (advisor), Whitford, Hayley (advisor), School of Psychology (school).

Subjects/Keywords: psychoneuroimmunology; stress; the oxidative model

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APA (6^th Edition):

Oliver-Baxter, J. M. (2011). The psychoneuroimmunology of women experiencing stressful life events: testing the oxidative model. (Thesis). University of Adelaide. Retrieved from http://hdl.handle.net/2440/74664

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Oliver-Baxter, Jodie Merle. “The psychoneuroimmunology of women experiencing stressful life events: testing the oxidative model.” 2011. Thesis, University of Adelaide. Accessed March 02, 2021. http://hdl.handle.net/2440/74664.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Oliver-Baxter, Jodie Merle. “The psychoneuroimmunology of women experiencing stressful life events: testing the oxidative model.” 2011. Web. 02 Mar 2021.

Vancouver:

Oliver-Baxter JM. The psychoneuroimmunology of women experiencing stressful life events: testing the oxidative model. [Internet] [Thesis]. University of Adelaide; 2011. [cited 2021 Mar 02]. Available from: http://hdl.handle.net/2440/74664.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Oliver-Baxter JM. The psychoneuroimmunology of women experiencing stressful life events: testing the oxidative model. [Thesis]. University of Adelaide; 2011. Available from: http://hdl.handle.net/2440/74664

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Toronto

19. Chiavaroli, Laura. Oxidative Stress and Risk of Cardiovascular Disease Associated with Low- and High-monounsaturated Fat Portfolio Diets.

Degree: 2010, University of Toronto

URL: http://hdl.handle.net/1807/24549

►

The objective was to assess the effect of a high-monounsaturated fat (MUFA) dietary portfolio of cholesterol-lowering foods on oxidative stress and cardiovascular risk. Twenty-four hyperlipidemic… (more)

Subjects/Keywords: oxidative stress; cholesterol; 0570

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APA (6^th Edition):

Chiavaroli, L. (2010). Oxidative Stress and Risk of Cardiovascular Disease Associated with Low- and High-monounsaturated Fat Portfolio Diets. (Masters Thesis). University of Toronto. Retrieved from http://hdl.handle.net/1807/24549

Chicago Manual of Style (16^th Edition):

Chiavaroli, Laura. “Oxidative Stress and Risk of Cardiovascular Disease Associated with Low- and High-monounsaturated Fat Portfolio Diets.” 2010. Masters Thesis, University of Toronto. Accessed March 02, 2021. http://hdl.handle.net/1807/24549.

MLA Handbook (7^th Edition):

Chiavaroli, Laura. “Oxidative Stress and Risk of Cardiovascular Disease Associated with Low- and High-monounsaturated Fat Portfolio Diets.” 2010. Web. 02 Mar 2021.

Vancouver:

Chiavaroli L. Oxidative Stress and Risk of Cardiovascular Disease Associated with Low- and High-monounsaturated Fat Portfolio Diets. [Internet] [Masters thesis]. University of Toronto; 2010. [cited 2021 Mar 02]. Available from: http://hdl.handle.net/1807/24549.

Council of Science Editors:

Chiavaroli L. Oxidative Stress and Risk of Cardiovascular Disease Associated with Low- and High-monounsaturated Fat Portfolio Diets. [Masters Thesis]. University of Toronto; 2010. Available from: http://hdl.handle.net/1807/24549

University of Manitoba

20. Morales, Maria. Fecal microbiome, feeding patterns and oxidative stress among preterm infants: an exploratory study.

Degree: Human Nutritional Sciences, 2016, University of Manitoba

URL: http://hdl.handle.net/1993/32032

► It is known that the birth process and initial life exposures, such as feeding, may have an important impact on the acquisition of bacterial communities… (more)

Subjects/Keywords: microbiome; oxidative stress; prematurity

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APA (6^th Edition):

Morales, M. (2016). Fecal microbiome, feeding patterns and oxidative stress among preterm infants: an exploratory study. (Masters Thesis). University of Manitoba. Retrieved from http://hdl.handle.net/1993/32032

Chicago Manual of Style (16^th Edition):

Morales, Maria. “Fecal microbiome, feeding patterns and oxidative stress among preterm infants: an exploratory study.” 2016. Masters Thesis, University of Manitoba. Accessed March 02, 2021. http://hdl.handle.net/1993/32032.

MLA Handbook (7^th Edition):

Morales, Maria. “Fecal microbiome, feeding patterns and oxidative stress among preterm infants: an exploratory study.” 2016. Web. 02 Mar 2021.

Vancouver:

Morales M. Fecal microbiome, feeding patterns and oxidative stress among preterm infants: an exploratory study. [Internet] [Masters thesis]. University of Manitoba; 2016. [cited 2021 Mar 02]. Available from: http://hdl.handle.net/1993/32032.

Council of Science Editors:

Morales M. Fecal microbiome, feeding patterns and oxidative stress among preterm infants: an exploratory study. [Masters Thesis]. University of Manitoba; 2016. Available from: http://hdl.handle.net/1993/32032

University of Exeter

21. Knight, Annie Rose. Measurement of nitric oxide metabolites and protein nitration in healthy and inflammatory human tissues and bio-fluids.

Degree: PhD, 2016, University of Exeter

URL: http://hdl.handle.net/10871/25578

► The central thesis of this project is that damage caused by reactive nitrogen species, e.g. 3-nitrotyrosine (Tyr-NO2), constitutes a marker of disease progression/severity. A new… (more)

▼ The central thesis of this project is that damage caused by reactive nitrogen species, e.g. 3-nitrotyrosine (Tyr-NO2), constitutes a marker of disease progression/severity. A new sensitive electrochemiluminescence ELISA was optimised and validated for Tyr-NO2 measurement, giving a lower limit of quantification of 0.04 nM BSA-NO2, intra- and inter-assay CVs of 6.5% and 11.3%, an average recovery of 106 ± 3% and average linearity 0.998 ± 0.001. Nitrative stress, carbonyl stress and C-reactive protein (CRP) concentrations were measured before and after major elective surgery. CRP measurements confirmed the induction of an inflammatory response. Median serum Tyr-NO2 levels increased post-surgery to a median (inter-quartile range) value of 0.97 (0 – 1.7) fmol nitrated BSA (BSA-NO2) equivalents/mg protein compared with a pre-surgery level of 0.59 (0 – 1.3) fmol BSA-NO2 equivalents/mg protein (p<0.05). Oxidative damage was confirmed by serum protein carbonyl levels (p<0.05). In a second pre-/post- surgery study, patients who developed sepsis postoperatively had significantly higher serum Tyr-NO2 levels one day prior to diagnosis (median (IQR) 4.5 (1.65 – 8.21) fmol BSA-NO2 equivalents/mg protein) compared to patients without sepsis (1.2 (0.74 – 5.97) fmol BSA-NO2 equivalents/mg protein; p<0.05). Tyr-NO2 levels have not previously been measured before clinical diagnosis. However, Tyr-NO2 did not improve upon CRP as a diagnostic marker (area under the curve: Tyr-NO2 0.69 versus CRP 0.88). Nitrate (NO3¯) supplementation in healthy smokers was also studied. Plasma Tyr-NO2 levels were unaltered by supplementation or smoking status. Salivary nitration was unaffected by smoking and decreased with NO3¯ supplementation: the median (IQR) pre-supplementation was 0.67 (0.31-1.14) and post-supplementation was 0.43 (0.12-0.61) pmol BSA-NO2 equivalents/mg protein. Ozone-based chemiluminescence was utilised for nitrite (NO2¯) and NO3¯ measurement as indicators of ˙NO production. Plasma and salivary NO2¯ and NO3¯ concentrations increased significantly with NO3¯ supplementation (p<0.05). In contrast to published studies, brain frontal lobe Tyr-NO2 levels were not higher in dementia: the median (IQR) levels in dementia were 0.29 (0.19-0.57) and in non-dementia controls were 0.3 (0.22-0.55) pmol BSA-NO2 equivalents/mg protein. However, the median brain tissue NO2¯ concentration was significantly higher in the Alzheimer’s disease group (p<0.05). Western blotting revealed that nitration was predominantly in a few select proteins, with TOF-MS/MS analysis suggesting haemoglobin is one of these proteins. Measurement of nitrative stress using ozone-based chemiluminescence and an electrochemiluminescence-based-ELISA overcomes earlier methodological flaws, such as low sensitivity. Detection of total Tyr-NO2 in different inflammatory states indicates that its measurement could have potential as a marker of disease, but measurement of nitration in specific proteins may be more informative than total Tyr-NO2.

Subjects/Keywords: 612.8; oxidative stress; nitrotyrosine; inflammation

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Knight, A. R. (2016). Measurement of nitric oxide metabolites and protein nitration in healthy and inflammatory human tissues and bio-fluids. (Doctoral Dissertation). University of Exeter. Retrieved from http://hdl.handle.net/10871/25578

Chicago Manual of Style (16^th Edition):

Knight, Annie Rose. “Measurement of nitric oxide metabolites and protein nitration in healthy and inflammatory human tissues and bio-fluids.” 2016. Doctoral Dissertation, University of Exeter. Accessed March 02, 2021. http://hdl.handle.net/10871/25578.

MLA Handbook (7^th Edition):

Knight, Annie Rose. “Measurement of nitric oxide metabolites and protein nitration in healthy and inflammatory human tissues and bio-fluids.” 2016. Web. 02 Mar 2021.

Vancouver:

Knight AR. Measurement of nitric oxide metabolites and protein nitration in healthy and inflammatory human tissues and bio-fluids. [Internet] [Doctoral dissertation]. University of Exeter; 2016. [cited 2021 Mar 02]. Available from: http://hdl.handle.net/10871/25578.

Council of Science Editors:

Knight AR. Measurement of nitric oxide metabolites and protein nitration in healthy and inflammatory human tissues and bio-fluids. [Doctoral Dissertation]. University of Exeter; 2016. Available from: http://hdl.handle.net/10871/25578

University of Dundee

22. Auciello, Francesca Romana. Canonical and non-canonical regulation of AMP-activated protein kinase.

Degree: PhD, 2015, University of Dundee

URL: https://discovery.dundee.ac.uk/en/studentTheses/2720a2b7-3f1e-445c-b008-c5c235f35395 ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.679087

► The AMP-activated protein kinase (AMPK) is a sensor of cellular energy stress that, once activated, promotes ATP-producing process while it switches off ATP-consuming pathways, in… (more)

▼ The AMP-activated protein kinase (AMPK) is a sensor of cellular energy stress that, once activated, promotes ATP-producing process while it switches off ATP-consuming pathways, in order to restore the cellular energetic balance under conditions of stress. Activation of AMPK is dependent on the phosphorylation of the residue Thr172 in its α subunit. This phosphorylation is generally mediated by the known tumour suppressor LKB1, but also CaMKKβ has been shown to phosphorylate AMPK. As its name suggests, AMPK is also activated by the binding of AMP to its γ subunit. This binding causes a >10 fold allosteric stimulation, promotes phosphorylation of Thr172 by upstream kinases and protects AMPK from dephosphorylation of Thr172 by protein phosphatase(s). In 2010 it was reported that oxidative stress mediated by H₂O₂ activated AMPK by increasing the cellular AMP:ATP and ADP:ATP ratios (Hawley et al, 2010). However, the same year another work suggested that the mechanism of activation of AMPK by H₂O₂was direct, independent of AMP and involved the oxidation of two cysteine residues in the α subunit of AMPK (Zmijewski et al, 2010). Given this discrepancy, here we provided evidence that H₂O₂, generated by addition of glucose oxidase in the cell medium, activates AMPK mostly through an increase of AMP:ATP and ADP:ATP ratios, as previously suggested in our laboratory. However, it seems that there might be a second, minor mechanism of activation that is independent of the changes in cellular nucleotides. This second mechanism was not identified in our previous work because we were not aware of how rapidly a single bolus of H₂O₂ can be metabolized by the antioxidant defences of the cell. We could not identify the alternative mechanism of activation by H₂O₂but showed that H₂O₂ could protect Thr172 from dephosphorylation, which might suggest a direct effect of H₂O₂ on the phosphatase(s) dephosphorylating AMPK. However, since the identity of this phosphatase(s) remains unclear, we could not rule out the possibility that the protection from dephosphorylation that we observed could still be mediated by the increase in AMP:ATP and ADP:ATP ratios. Moreover, it remains still possible that a direct effect of H₂O₂ on AMPK might be responsible for the small but significant activation we detected in cell expressing a nucleotides-insensitive mutant of AMPK. Recently, a new crystal structure of AMPK obtained by Xiao et al (2013) provided new insights about AMPK structure and regulation. In particular, the authors identified a new binding pocket located at the interface between the N-lobe of the α-kinase domain and the β-CBM of AMPK, which appeared to be the binding site for two direct activators of AMPK: A769662 and 991. Here we confirm that this novel binding pocket is indeed the binding site for both A769662 and 991, and provide evidence that another direct activator of…

Subjects/Keywords: 616; AMPK; Oxidative stress

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APA (6^th Edition):

Auciello, F. R. (2015). Canonical and non-canonical regulation of AMP-activated protein kinase. (Doctoral Dissertation). University of Dundee. Retrieved from https://discovery.dundee.ac.uk/en/studentTheses/2720a2b7-3f1e-445c-b008-c5c235f35395 ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.679087

Chicago Manual of Style (16^th Edition):

Auciello, Francesca Romana. “Canonical and non-canonical regulation of AMP-activated protein kinase.” 2015. Doctoral Dissertation, University of Dundee. Accessed March 02, 2021. https://discovery.dundee.ac.uk/en/studentTheses/2720a2b7-3f1e-445c-b008-c5c235f35395 ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.679087.

MLA Handbook (7^th Edition):

Auciello, Francesca Romana. “Canonical and non-canonical regulation of AMP-activated protein kinase.” 2015. Web. 02 Mar 2021.

Vancouver:

Auciello FR. Canonical and non-canonical regulation of AMP-activated protein kinase. [Internet] [Doctoral dissertation]. University of Dundee; 2015. [cited 2021 Mar 02]. Available from: https://discovery.dundee.ac.uk/en/studentTheses/2720a2b7-3f1e-445c-b008-c5c235f35395 ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.679087.

Council of Science Editors:

Auciello FR. Canonical and non-canonical regulation of AMP-activated protein kinase. [Doctoral Dissertation]. University of Dundee; 2015. Available from: https://discovery.dundee.ac.uk/en/studentTheses/2720a2b7-3f1e-445c-b008-c5c235f35395 ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.679087

Victoria University of Wellington

23. Quek, Natelle C H. The Characterization of TA-289, a Novel Antifungal from Fusarium sp.

Degree: 2011, Victoria University of Wellington

URL: http://hdl.handle.net/10063/1891

► Natural products offer vast structural and chemical diversity highly sought after in drug discovery research. Saccharomyces cerevisiae makes an ideal model eukaryotic organism for drug… (more)

▼ Natural products offer vast structural and chemical diversity highly sought after in drug discovery research. Saccharomyces cerevisiae makes an ideal model eukaryotic organism for drug mode-of-action studies owing to ease of growth, sophistication of genetic tools and overall homology to higher eukaryotes. Equisetin and a closely related novel natural product, TA-289, are cytotoxic to fermenting yeast, but seemingly less so when yeast actively respire. Cell cycle analyses by flow cytometry revealed a cell cycle block at S-G2/M phase caused by TA-289; previously described oxidative stress-inducing compounds causing cell cycle delay led to further investigation in the involvement of equisetin and TA-289 in mitochondrial-mediated generation of reactive oxygen species. Chemical genomic profiling involving genome-wide scans of yeast deletion mutant strains for TA-289 sensitivity revealed sensitization of genes involved in the mitochondria, DNA damage repair and oxidative stress responses, consistent with a possible mechanism-of-action at the mitochondrion. Flow cytometric detection of reactive oxygen species (ROS) generation caused by TA-289 suggests that the compound may induce cell death via ROS production. The generation of a mutant strain resistant to TA-289 also displayed resistance to a known oxidant, H2O2, at concentrations that were cytotoxic to wild-type cells. The resistant mutant displayed a higher basal level of ROS production compared to the wild-type parent, indicating that the resistance mutation led to an up-regulation of antioxidant capacity which provides cell survival in the presence of TA-289. Yeast mitochondrial morphology was visualized by confocal light microscopy, where it was observed that cells treated with TA-289 displayed abnormal mitochondria phenotypes, further indicating that the compound is acting primarily at the mitochondrion. Similar effects observed with equisetin treatment suggest that both compounds share the same mechanism, eliciting cell death via ROS production in the mitochondrial respiratory chain. Advisors/Committee Members: Bellows, David, Atkinson, Paul.

Subjects/Keywords: Drug; Oxidative stress; Saccharomyces cerevisiae

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APA (6^th Edition):

Quek, N. C. H. (2011). The Characterization of TA-289, a Novel Antifungal from Fusarium sp. (Masters Thesis). Victoria University of Wellington. Retrieved from http://hdl.handle.net/10063/1891

Chicago Manual of Style (16^th Edition):

Quek, Natelle C H. “The Characterization of TA-289, a Novel Antifungal from Fusarium sp.” 2011. Masters Thesis, Victoria University of Wellington. Accessed March 02, 2021. http://hdl.handle.net/10063/1891.

MLA Handbook (7^th Edition):

Quek, Natelle C H. “The Characterization of TA-289, a Novel Antifungal from Fusarium sp.” 2011. Web. 02 Mar 2021.

Vancouver:

Quek NCH. The Characterization of TA-289, a Novel Antifungal from Fusarium sp. [Internet] [Masters thesis]. Victoria University of Wellington; 2011. [cited 2021 Mar 02]. Available from: http://hdl.handle.net/10063/1891.

Council of Science Editors:

Quek NCH. The Characterization of TA-289, a Novel Antifungal from Fusarium sp. [Masters Thesis]. Victoria University of Wellington; 2011. Available from: http://hdl.handle.net/10063/1891

University of Toronto

24. Sarkar, Susmita. The Role Of Oxidative Stress Following Antenatal Synthetic Glucocorticoid Therapy.

Degree: 2018, University of Toronto

URL: http://hdl.handle.net/1807/102832

►

We investigated the role of oxidative stress following sGC treatment in a guinea pig model of gestation through measures including: expression of antioxidant enzyme genes,… (more)

Subjects/Keywords: Antenatal; Glucocorticoid; Oxidative stress; 0719

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Sarkar, S. (2018). The Role Of Oxidative Stress Following Antenatal Synthetic Glucocorticoid Therapy. (Masters Thesis). University of Toronto. Retrieved from http://hdl.handle.net/1807/102832

Chicago Manual of Style (16^th Edition):

Sarkar, Susmita. “The Role Of Oxidative Stress Following Antenatal Synthetic Glucocorticoid Therapy.” 2018. Masters Thesis, University of Toronto. Accessed March 02, 2021. http://hdl.handle.net/1807/102832.

MLA Handbook (7^th Edition):

Sarkar, Susmita. “The Role Of Oxidative Stress Following Antenatal Synthetic Glucocorticoid Therapy.” 2018. Web. 02 Mar 2021.

Vancouver:

Sarkar S. The Role Of Oxidative Stress Following Antenatal Synthetic Glucocorticoid Therapy. [Internet] [Masters thesis]. University of Toronto; 2018. [cited 2021 Mar 02]. Available from: http://hdl.handle.net/1807/102832.

Council of Science Editors:

Sarkar S. The Role Of Oxidative Stress Following Antenatal Synthetic Glucocorticoid Therapy. [Masters Thesis]. University of Toronto; 2018. Available from: http://hdl.handle.net/1807/102832

East Carolina University

25. Brock, Megan Elizabeth. Transcriptional Analysis of the Bacteroides fragilis Starch Utilization Operon osuA.

Degree: 2011, East Carolina University

URL: http://libres.uncg.edu/ir/listing.aspx?styp=ti&id=14805

► The opportunistic pathogen Bacteroides fragilis is a symbiotic organism that inhabits the human gastrointestinal tract where it utilizes dietary and host-derived polysaccharides as carbon and… (more)

▼ The opportunistic pathogen Bacteroides fragilis is a symbiotic organism that inhabits the human gastrointestinal tract where it utilizes dietary and host-derived polysaccharides as carbon and energy sources. If abdominal injury occurs this otherwise commensal organism can migrate from the anaerobic environment of the large intestine to the more aerobic peritoneum. In this new extraintestinal environment B. fragilis frequently contributes to the development of intra-abdominal abscesses and is often the most common isolate from such anaerobic infections which can lead to systemic infections and death if left untreated. The organism's ability to shift from commensalist to pathogen is inextricably linked with the complex oxidative stress response (OSR) it has evolved. The studies described in this thesis have focused on the characterization of the promoter for the oxidative starch utilization operon osu and the identification of regulatory sequences involved in transcription activation during growth in maltose or exposure to oxygen. The results of this promoter deletional analysis study have demonstrated that the osu promoter is indeed responsive to both maltose and oxygen and that regulatory regions important for activation of transcription in response to both stimuli are likely found within the same 50 bp region of the promoter. Consistent with this observation was the discovery of a LacI-type binding region in this site. In addition studies demonstrated that there is the possibility of an additional weak oxygen-responsive promoter that exists in a separate region of the osu promoter. The previously identified transcriptional activator OsuR may also play a critical role in transcription activation of the osu promoter regardless of whether the inducing agent is maltose or oxygen. The mechanism of osu protection during oxidative stress is not fully understood but results of this thesis offer a more complex model of transcriptional activation of the osu operon than was initially theorized. More studies will be necessary to further elucidate the role of osu in maintaining the OSR of B. fragilis during oxygen exposure. ; Microbiology, Molecular biology, Genetics Advisors/Committee Members: C. Jeffrey Smith (advisor).

Subjects/Keywords: Bacteroides; Symbiosis; Oxidative stress

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APA (6^th Edition):

Brock, M. E. (2011). Transcriptional Analysis of the Bacteroides fragilis Starch Utilization Operon osuA. (Masters Thesis). East Carolina University. Retrieved from http://libres.uncg.edu/ir/listing.aspx?styp=ti&id=14805

Chicago Manual of Style (16^th Edition):

Brock, Megan Elizabeth. “Transcriptional Analysis of the Bacteroides fragilis Starch Utilization Operon osuA.” 2011. Masters Thesis, East Carolina University. Accessed March 02, 2021. http://libres.uncg.edu/ir/listing.aspx?styp=ti&id=14805.

MLA Handbook (7^th Edition):

Brock, Megan Elizabeth. “Transcriptional Analysis of the Bacteroides fragilis Starch Utilization Operon osuA.” 2011. Web. 02 Mar 2021.

Vancouver:

Brock ME. Transcriptional Analysis of the Bacteroides fragilis Starch Utilization Operon osuA. [Internet] [Masters thesis]. East Carolina University; 2011. [cited 2021 Mar 02]. Available from: http://libres.uncg.edu/ir/listing.aspx?styp=ti&id=14805.

Council of Science Editors:

Brock ME. Transcriptional Analysis of the Bacteroides fragilis Starch Utilization Operon osuA. [Masters Thesis]. East Carolina University; 2011. Available from: http://libres.uncg.edu/ir/listing.aspx?styp=ti&id=14805

26. Leetham, Mallory Spencer. Lymphocyte subpopulations and oxidative stress following sub-acute exposure to natural dust collected from the Nellis Dunes Recreational Area.

Degree: MS, College of Letters & Science, 2015, Montana State University

URL: https://scholarworks.montana.edu/xmlui/handle/1/9057

► Exposure to particulate matter containing heavy metals has been linked to adverse health effects when exposure occurs in industrial settings; however, little data exist on… (more)

▼ Exposure to particulate matter containing heavy metals has been linked to adverse health effects when exposure occurs in industrial settings; however, little data exist on effects associated with natural exposure settings. In this study, markers of oxidative stress and lymphocyte subpopulations in mice were observed following sub-acute exposure to metals-containing dust collected from a natural setting used heavily for off-road vehicle (ORV) recreation. Adult female B6C3F1 mice were exposed to concentrations of dust collected from seven types of surfaces at the Nellis Dunes Recreation Area. Dust representing each of the seven map units was prepared with a median diameter of < or = 4.5m and suspended in PBS immediately prior to oropharyngeal aspiration at concentrations from 0.01 - 100 mg of dust/kg body weight. Four exposures were given a week apart over 28-days to mimic a month of weekend exposures. Thymi, spleens and blood for evaluation of oxidative stress markers and lymphocyte sub-populations were collected 24 hours after the final exposure. Blood markers of oxidative stress included levels of free radicals, superoxide dismutase, total antioxidant capacity, and total glutathione. CD4, CD8, FoxP3, CD25, IL-17, and B220 cell surface markers were used for T and B cell identification using flow cytometry. Overall, no single surface type was able to consistently induce markers of oxidative stress at a particular dose or in a dose-responsive manner. The two highest concentrations of dust from one surface type increased two markers of oxidative stress, but results of other surface types were inconsistent. No statistically significant changes were observed in the splenic B220+ cells following NDRA dust exposure. Three CBN units (1, 2, and 6) showed decreases in splenic CD4+/CD25+/FoxP3- cells. These observations were relatively consistent with TiO 2, where a significant change at the highest exposure level was observed in only one measure of oxidative stress. Additionally, the TiO 2 dosing groups showed no significant changes in lymphocyte subpopulations. These results indicate that exposure to these natural, mineral dusts, under the exposure scenario of our study, while are unlikely to considerably increase the risk of oxidative damage systemically, may induce a reduction in some cell populations in exposed individuals. Advisors/Committee Members: Chairperson, Graduate Committee: Deborah Keil (advisor), Deborah Keil and Jamie DeWitt were co-authors of the article, 'Oxidative stress and lung histopathology following sub-acute exposure to natural dust collected from the Nellis Dunes Recreation Area' submitted to the journal 'Bureau of Land Management Report' which is contained within this thesis. (other).

Subjects/Keywords: Mineral dusts.; Respiration.; Oxidative stress.

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APA (6^th Edition):

Leetham, M. S. (2015). Lymphocyte subpopulations and oxidative stress following sub-acute exposure to natural dust collected from the Nellis Dunes Recreational Area. (Masters Thesis). Montana State University. Retrieved from https://scholarworks.montana.edu/xmlui/handle/1/9057

Chicago Manual of Style (16^th Edition):

Leetham, Mallory Spencer. “Lymphocyte subpopulations and oxidative stress following sub-acute exposure to natural dust collected from the Nellis Dunes Recreational Area.” 2015. Masters Thesis, Montana State University. Accessed March 02, 2021. https://scholarworks.montana.edu/xmlui/handle/1/9057.

MLA Handbook (7^th Edition):

Leetham, Mallory Spencer. “Lymphocyte subpopulations and oxidative stress following sub-acute exposure to natural dust collected from the Nellis Dunes Recreational Area.” 2015. Web. 02 Mar 2021.

Vancouver:

Leetham MS. Lymphocyte subpopulations and oxidative stress following sub-acute exposure to natural dust collected from the Nellis Dunes Recreational Area. [Internet] [Masters thesis]. Montana State University; 2015. [cited 2021 Mar 02]. Available from: https://scholarworks.montana.edu/xmlui/handle/1/9057.

Council of Science Editors:

Leetham MS. Lymphocyte subpopulations and oxidative stress following sub-acute exposure to natural dust collected from the Nellis Dunes Recreational Area. [Masters Thesis]. Montana State University; 2015. Available from: https://scholarworks.montana.edu/xmlui/handle/1/9057

University of Minnesota

27. Peterson, Katie. Lanthanide-Based Probes for Oxidative Stress.

Degree: PhD, Chemistry, 2014, University of Minnesota

URL: http://hdl.handle.net/11299/191469

► Oxidative stress, or the imbalance of reactive oxidative species and antioxidants, is implicated in a wide variety of physiological functions and diseases. Currently, little is… (more)

▼ Oxidative stress, or the imbalance of reactive oxidative species and antioxidants, is implicated in a wide variety of physiological functions and diseases. Currently, little is known about the biological concentrations and the exact roles of individual species. In particular, the cellular concentration of hydroxyl radical and the etiology of this reactive oxygen species in disease states are unclear. The photophysical properties of luminescent lanthanide-based imaging agents and the magnetic properties of fluorinated contrast agents make them favorable candidates to monitor oxidative species in biological environments. Luminescent lanthanide-based probes for hydroxyl radical are presented. These probes utilize aromatic acid pre-antennas that sensitize terbium emission upon hydroxylation. The ability of hydroxylated and non-hydroxylated aromatic acids including benzoate, benzamide, isophthalate, isophthalamide, trimesate, and trimesamide to sensitize Tb DO3A was evaluated by time-delayed luminescence spectroscopy. The formation of a weak ternary complex between hydroxytrimeasamide and Tb-DO3A was confirmed by temperature-dependent titrations. The luminescence response of the bimolecular Tb DO3A and trimesamide probe to hydroxyl radical generated by the photolysis of hydrogen peroxide was investigated. The system exhibits excellent selectivity for hydroxyl radical over other biologically relevant reactive oxygen and nitrogen species. Next, fluorinated magnetic resonance imaging contrast agents responsive to hydroxyl radical are described. The 3,5-difluorobenzoic acid probe is water soluble and ratiometrically responds to hydroxyl radical. Upon hydroxylation, a fluoride ion is released. The relative signal intensity of the product and that of the unreacted contrast agent can then be used to monitor the analyte in a ratiometric manner by 19F NMR and 19F MRI. The selectivity of the system towards hydroxyl radical compared to other reactive oxygen and nitrogen species is also measured. Paramagnetic, lanthanide-based contrast agents incorporating the sensing moiety are also evaluated for increased sensitivity of detection compared to the diamagnetic analogs. Additionally, a family of lanthanide-based luminescent complexes based on a macrocyclic core featuring different sensitizing antennas and variable pendant arms are investigated in terms of their biological compatibility. The cellular uptake of Tb-DOTA complexes containing hydroxyisophthalamide (IAM), methoxyisophthalamide (IAM(OMe)), or phenathridine (Phen) antenna were comparable despite their differences in hydrophobicity. The luminescence quenching of Tb-DOTA-IAM(OMe) was also investigated in cell lysate by time-delayed spectroscopy. Pendant arms varying in hydrophobicity and charge were used to evaluate the effect of structural and electronic properties on cellular viability and cell association as measured by a MTT assay and ICP-MS, respectively. Regardless of the amide substituents, complexes based on Tb-DOTAm-IAM(OMe) core exhibited low…

Subjects/Keywords: Imaging; Lanthanide; Oxidative Stress

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APA (6^th Edition):

Peterson, K. (2014). Lanthanide-Based Probes for Oxidative Stress. (Doctoral Dissertation). University of Minnesota. Retrieved from http://hdl.handle.net/11299/191469

Chicago Manual of Style (16^th Edition):

Peterson, Katie. “Lanthanide-Based Probes for Oxidative Stress.” 2014. Doctoral Dissertation, University of Minnesota. Accessed March 02, 2021. http://hdl.handle.net/11299/191469.

MLA Handbook (7^th Edition):

Peterson, Katie. “Lanthanide-Based Probes for Oxidative Stress.” 2014. Web. 02 Mar 2021.

Vancouver:

Peterson K. Lanthanide-Based Probes for Oxidative Stress. [Internet] [Doctoral dissertation]. University of Minnesota; 2014. [cited 2021 Mar 02]. Available from: http://hdl.handle.net/11299/191469.

Council of Science Editors:

Peterson K. Lanthanide-Based Probes for Oxidative Stress. [Doctoral Dissertation]. University of Minnesota; 2014. Available from: http://hdl.handle.net/11299/191469

28. Cates- Gatto, Chelsea. Effects of Zinc on Ethanol Drinking, Behavior, and Oxidative Stress Following Chronic Intermittent Ethanol Exposure .

Degree: 2013, California State University – San Marcos

URL: http://hdl.handle.net/10211.8/419

► The negative effects associated with increased alcohol consumption have been well documented, and include increased risk for depression, anxiety, sleep disorders, stroke, heart disease, certain… (more)

Subjects/Keywords: Ethanol; oxidative stress; mouse; behavior

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APA (6^th Edition):

Cates- Gatto, C. (2013). Effects of Zinc on Ethanol Drinking, Behavior, and Oxidative Stress Following Chronic Intermittent Ethanol Exposure . (Thesis). California State University – San Marcos. Retrieved from http://hdl.handle.net/10211.8/419

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Cates- Gatto, Chelsea. “Effects of Zinc on Ethanol Drinking, Behavior, and Oxidative Stress Following Chronic Intermittent Ethanol Exposure .” 2013. Thesis, California State University – San Marcos. Accessed March 02, 2021. http://hdl.handle.net/10211.8/419.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Cates- Gatto, Chelsea. “Effects of Zinc on Ethanol Drinking, Behavior, and Oxidative Stress Following Chronic Intermittent Ethanol Exposure .” 2013. Web. 02 Mar 2021.

Vancouver:

Cates- Gatto C. Effects of Zinc on Ethanol Drinking, Behavior, and Oxidative Stress Following Chronic Intermittent Ethanol Exposure . [Internet] [Thesis]. California State University – San Marcos; 2013. [cited 2021 Mar 02]. Available from: http://hdl.handle.net/10211.8/419.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Cates- Gatto C. Effects of Zinc on Ethanol Drinking, Behavior, and Oxidative Stress Following Chronic Intermittent Ethanol Exposure . [Thesis]. California State University – San Marcos; 2013. Available from: http://hdl.handle.net/10211.8/419

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

29. Tuet, Wing-Yin. Cellular and acellular assays for measuring oxidative stress induced by ambient and laboratory-generated aerosol.

Degree: PhD, Chemical and Biomolecular Engineering, 2018, Georgia Tech

URL: http://hdl.handle.net/1853/59870

► Exposure to atmospheric particulate matter (PM) is a leading global health risk with various proposed mechanisms of action, including the induction of oxidative stress through… (more)

▼ Exposure to atmospheric particulate matter (PM) is a leading global health risk with various proposed mechanisms of action, including the induction of oxidative stress through PM-initiated production/release of reactive oxygen and nitrogen species (ROS/RNS). This dissertation explores cellular and acellular measurements of PM-induced oxidative stress through systematic laboratory chamber experiments and ambient field studies. A cell-based assay involving murine alveolar macrophages was developed to measure intracellular ROS/RNS produced as a result of aerosol exposure. The area under the dose-response curve was identified as a robust metric to represent ROS/RNS for comparison with different endpoints. A large ambient study with samples collected from urban and rural sites around the greater Atlanta area (n = 104) was conducted using the optimized assay and significant correlations between ROS/RNS and organic constituents were observed for summer samples, highlighting the potential contribution of organic aerosol, particularly summertime photochemically-driven secondary organic aerosol (SOA). To explore these findings, SOA was generated in a series of laboratory experiments from various biogenic (isoprene, α-pinene, β-caryophyllene) and anthropogenic (pentadecane, m-xylene, naphthalene) precursors under different formation conditions (dry vs. humid, NOx, ammonium sulfate vs. iron sulfate seed particles) to probe their effects on PM toxicity. For chemical oxidative potential as measured by dithiothreitol consumption (OP), precursor identity influenced toxicity significantly, with isoprene and naphthalene SOA having the lowest and highest OP, respectively. Both precursor identity and formation conditions influenced ROS/RNS and cytokine (tumor necrosis factor-α and interleukin-6) production. Several response patterns were identified for SOA precursors whose photooxidation products share similar carbon chain length and functionalities. A significant correlation between ROS/RNS levels and aerosol carbon oxidation state was also observed, which may have significant implications as atmospheric aerosol have an atmospheric lifetime of a week, over which oxidation state increases due to photochemical aging, potentially resulting in more toxic aerosol. Advisors/Committee Members: Ng, Nga L. (advisor), Champion, Julie A. (committee member), Grosberg, Anna (committee member), Lu, Hang (committee member), Weber, Rodney J. (committee member).

Subjects/Keywords: Particulate matter; Oxidative stress

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APA (6^th Edition):

Tuet, W. (2018). Cellular and acellular assays for measuring oxidative stress induced by ambient and laboratory-generated aerosol. (Doctoral Dissertation). Georgia Tech. Retrieved from http://hdl.handle.net/1853/59870

Chicago Manual of Style (16^th Edition):

Tuet, Wing-Yin. “Cellular and acellular assays for measuring oxidative stress induced by ambient and laboratory-generated aerosol.” 2018. Doctoral Dissertation, Georgia Tech. Accessed March 02, 2021. http://hdl.handle.net/1853/59870.

MLA Handbook (7^th Edition):

Tuet, Wing-Yin. “Cellular and acellular assays for measuring oxidative stress induced by ambient and laboratory-generated aerosol.” 2018. Web. 02 Mar 2021.

Vancouver:

Tuet W. Cellular and acellular assays for measuring oxidative stress induced by ambient and laboratory-generated aerosol. [Internet] [Doctoral dissertation]. Georgia Tech; 2018. [cited 2021 Mar 02]. Available from: http://hdl.handle.net/1853/59870.

Council of Science Editors:

Tuet W. Cellular and acellular assays for measuring oxidative stress induced by ambient and laboratory-generated aerosol. [Doctoral Dissertation]. Georgia Tech; 2018. Available from: http://hdl.handle.net/1853/59870

Louisiana State University

30. Nguyen, Khoa Huynh. The roles of Deinococcus radiodurans Dps-1 and Dps-2 in nucleoid organization and in survival during oxidative stress.

Degree: PhD, 2013, Louisiana State University

URL: etd-03042013-205101 ; https://digitalcommons.lsu.edu/gradschool_dissertations/2659

► DNA protection during starvation (Dps) proteins are important for bacterial oxidative stress responses. This study aims to understand the roles and characteristics of the two… (more)

▼ DNA protection during starvation (Dps) proteins are important for bacterial oxidative stress responses. This study aims to understand the roles and characteristics of the two Dps homologs in Deinococcus radiodurans: Dps-1 and Dps-2. Dps-2 contains a predicted signal peptide and in vivo localization of Dps-2 reveals that its location is non-cytoplasmic. β-galactosidase assays show that the Dps-2 promoter is upregulated in the presence of H2O2 and the results from the DNA protection assay show that Dps-2 is able to protect DNA efficiently against reactive oxygen species (ROS). Dps-2 has a C-terminal extension that is needed for assembly into a dodecamer but not for DNA binding. Dps-1 is assembled from six dimers and stoichiometric experiments reveal that the stoichiometry of Dps-1 binding to 22 bp DNA substrate is 1:6, meaning that Dps-1has six DNA binding sites. However, for the 26 bp DNA substrate, the stoichiometry is 1:4, suggesting that the protein can interact with both faces of a DNA duplex provided there are two consecutive major grooves on each face. Furthermore, mutation of the surface arginine (Arg132) causes a decrease in DNA binding, which indicates that this residue is involved in the path of DNA binding of Dps-1 after the initial contact is made with the N-terminus. A model for the mode of Dps-1 binding to genomic DNA is proposed based on these observations. Dps-1 has a unique metal site at the end of the N-terminal extension and mutations of this site cause the protein to exist as a hexamer and this lead to a significant reduction in DNA binding. The mutant protein (Dps-HE) breaks down into dimers and loses its ability to bind DNA upon removal of divalent metals, wherease removal of metals from full-length Dps-1 has no effect on oligomeric state. These findings suggest that the N-terminal metal site is needed for proper assembly, but once the protein oligomerizes to a dodecamer, metals are no longer required to maintain the dodecameric state. These results suggest that the role of Dps-1 might be to organize genomic DNA while the role of Dps-2 might be to provide protection against incoming ROS.

Subjects/Keywords: deinococcus; dps; oxidative stress

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APA (6^th Edition):

Nguyen, K. H. (2013). The roles of Deinococcus radiodurans Dps-1 and Dps-2 in nucleoid organization and in survival during oxidative stress. (Doctoral Dissertation). Louisiana State University. Retrieved from etd-03042013-205101 ; https://digitalcommons.lsu.edu/gradschool_dissertations/2659

Chicago Manual of Style (16^th Edition):

Nguyen, Khoa Huynh. “The roles of Deinococcus radiodurans Dps-1 and Dps-2 in nucleoid organization and in survival during oxidative stress.” 2013. Doctoral Dissertation, Louisiana State University. Accessed March 02, 2021. etd-03042013-205101 ; https://digitalcommons.lsu.edu/gradschool_dissertations/2659.

MLA Handbook (7^th Edition):

Nguyen, Khoa Huynh. “The roles of Deinococcus radiodurans Dps-1 and Dps-2 in nucleoid organization and in survival during oxidative stress.” 2013. Web. 02 Mar 2021.

Vancouver:

Nguyen KH. The roles of Deinococcus radiodurans Dps-1 and Dps-2 in nucleoid organization and in survival during oxidative stress. [Internet] [Doctoral dissertation]. Louisiana State University; 2013. [cited 2021 Mar 02]. Available from: etd-03042013-205101 ; https://digitalcommons.lsu.edu/gradschool_dissertations/2659.

Council of Science Editors:

Nguyen KH. The roles of Deinococcus radiodurans Dps-1 and Dps-2 in nucleoid organization and in survival during oxidative stress. [Doctoral Dissertation]. Louisiana State University; 2013. Available from: etd-03042013-205101 ; https://digitalcommons.lsu.edu/gradschool_dissertations/2659

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Open Access Theses and Dissertations