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You searched for subject:(O glycan peeling). One record found.

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Leiden University

1. Kozak, R.P. Rapid and sensitive methods for the analysis and identification of O-glycans from glycoproteins.

Degree: 2017, Leiden University

Changes in both N- and O-glycosylation have been associated with many states of health and disease, providing prognostic and diagnostic information. O-glycans have been relatively little studied through lack of suitable analytical tools but the evidence is that they could be promising biomarkers. The release and analysis of O-glycans remains very challenging. The main reason for this is that there is no enzyme available for universal O-glycan release. Therefore, chemical release is the most effective method. Unfortunately all of the O-glycan release methods show a side reaction known as “peeling”. Peeling is well known problem when performing the O-glycan release and often results in poor repeatability. The aim of the work described in this thesis was to develop or improve methods for the release of O-glycans with free reducing termini with high yield and limited degradation. The development of sample preparation techniques for O-glycan release (in their nonreduced form) by hydrazinolysis are described. The use of procainamide for fluorescent labelling of glycans followed by HILIC-UHPLC-ESI-MS/MS analysis is introduced. The application of the improved protocols for O-glycan release by hydrazinolysis combined with developed procainamide labelling system followed by HILIC-UHPLC-ESI-MS/MS analysis for evaluation of O-glycosylation changes in human saliva. Advisors/Committee Members: Supervisor: A.M. Deelder, Fernandes, M. Wuhrer Co-Supervisor: D.L..

Subjects/Keywords: O-linked glycans; Hydrazinolysis; O-glycan peeling; Glycan release; Procainamide; HILIC–(U)HPLC; ESI–MS; Saliva; O-linked glycans; Hydrazinolysis; O-glycan peeling; Glycan release; Procainamide; HILIC–(U)HPLC; ESI–MS; Saliva

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6th Edition):

Kozak, R. P. (2017). Rapid and sensitive methods for the analysis and identification of O-glycans from glycoproteins. (Doctoral Dissertation). Leiden University. Retrieved from http://hdl.handle.net/1887/45434

Chicago Manual of Style (16th Edition):

Kozak, R P. “Rapid and sensitive methods for the analysis and identification of O-glycans from glycoproteins.” 2017. Doctoral Dissertation, Leiden University. Accessed December 12, 2019. http://hdl.handle.net/1887/45434.

MLA Handbook (7th Edition):

Kozak, R P. “Rapid and sensitive methods for the analysis and identification of O-glycans from glycoproteins.” 2017. Web. 12 Dec 2019.

Vancouver:

Kozak RP. Rapid and sensitive methods for the analysis and identification of O-glycans from glycoproteins. [Internet] [Doctoral dissertation]. Leiden University; 2017. [cited 2019 Dec 12]. Available from: http://hdl.handle.net/1887/45434.

Council of Science Editors:

Kozak RP. Rapid and sensitive methods for the analysis and identification of O-glycans from glycoproteins. [Doctoral Dissertation]. Leiden University; 2017. Available from: http://hdl.handle.net/1887/45434

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