subject:(Nucleotides)

Oregon State University

1. Albin, Edward Elmo. Cyclic nucleotides, cyclic nucleotide phosphodiesterase, protein kinase, and phosphoprotein phosphatase in the central nervous sytem of Manduca sexta.

Degree: PhD, Biochemistry and Biophysics, 1973, Oregon State University

URL: http://hdl.handle.net/1957/45233

► Adenosine 3', 5' -cyclic monophosphate (cyclic AMP, cAMP) and guanosine 3', 5' -cyclic monophosphate (cyclic GMP, cGMP) were quantitated in the central nervous system (CNS)… (more)

▼ Adenosine 3', 5' -cyclic monophosphate (cyclic AMP, cAMP) and guanosine 3', 5' -cyclic monophosphate (cyclic GMP, cGMP) were quantitated in the central nervous system (CNS) of the insect Manduca sexta by competitive protein binding techniques. The M. sexta CNS was found to contain a strikingly high level of cGMP, about 100-fold greater than that of mammalian brain. The level of cAMP, however, was estimated to be about one-sixth that of mammalian brain. The basal ratio of cGMP/cAMP in the insect CNS was approximately ten, whereas this ratio has been reported to be less than one in a variety of vertebrate and invertebrate tissues. Acetylcholine caused a great elevation of cGMP, but not cAMP, in the M. sexta CNS. Short-term incubation with ecdysterone (insect metamorphosis hormone) promoted the accumulation of both cyclic nucleotides. The existence of cyclic nucleotide- stimulable protein kinases (ATP: protein phosphotransferase, EC 2. 7. 1. 37), a system of enzymes postulated to be instrumental in the biochemical expression of cAMP and cGMP, was demonstrated in the CNS of both larval and adult M. sexta. At low concentrations, cAMP was a much more effective activator of kinase activity than cGMP, Cyclic AMP lowered the Km of the CNS kinase for ATP, a phenomenon which has been reported to be unique to nervous tissue in mammals. A number of the enzymological properties of the insect kinase were similar to those reported in the literature for this enzyme in mammalian tissues. This insect CNS was also shown to possess a potent enzyme system, viz. phosphoprotein phosphatase, for the dephosphorylation of the phosphorylated products of kinase. Phosphatase activity was gauged using phosphoprotamine as substrate. Both kinase and phosphatase activities were found to be enriched in particulate fractions of the CNS. An enzyme system for the destruction of cAMP and cGMP, cyclic nucleotide phosphodiesterase (nucleoside 3', 5' -cyclic phosphate nucleoside 5' -phosphate 31 -phosphohydrolase, EC 3.1. 4< c), was examined in the M. sexta larval and adult CNS. Phosphodiesterase (PDE) was found in both soluble and particulate fractions of the CNS, but highest specific activity PDE was noted in a crude mitochondrial preparation, a fraction presumably containing synaptic elements.PDE was greatly enriched in the brain relative to the other CNS ganglia, and was present at higher levels in nervous relative to nonnervous tissues. The hydrolysis of both cAMP and cGMP appeared to be the function of a single enzyme (or similar isozymes) in the larval CNS. Kinetic evidence suggested that PDE in the insect is a cooperative enzyme and is characterized by non-linear, biphasic double-reciprocal plots. PDE could be effectively inhibited by presumed physiological levels of ATP. Advisors/Committee Members: Newburgh, R. W. (advisor).

Subjects/Keywords: Nucleotides

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APA (6^th Edition):

Albin, E. E. (1973). Cyclic nucleotides, cyclic nucleotide phosphodiesterase, protein kinase, and phosphoprotein phosphatase in the central nervous sytem of Manduca sexta. (Doctoral Dissertation). Oregon State University. Retrieved from http://hdl.handle.net/1957/45233

Chicago Manual of Style (16^th Edition):

Albin, Edward Elmo. “Cyclic nucleotides, cyclic nucleotide phosphodiesterase, protein kinase, and phosphoprotein phosphatase in the central nervous sytem of Manduca sexta.” 1973. Doctoral Dissertation, Oregon State University. Accessed March 06, 2021. http://hdl.handle.net/1957/45233.

MLA Handbook (7^th Edition):

Albin, Edward Elmo. “Cyclic nucleotides, cyclic nucleotide phosphodiesterase, protein kinase, and phosphoprotein phosphatase in the central nervous sytem of Manduca sexta.” 1973. Web. 06 Mar 2021.

Vancouver:

Albin EE. Cyclic nucleotides, cyclic nucleotide phosphodiesterase, protein kinase, and phosphoprotein phosphatase in the central nervous sytem of Manduca sexta. [Internet] [Doctoral dissertation]. Oregon State University; 1973. [cited 2021 Mar 06]. Available from: http://hdl.handle.net/1957/45233.

Council of Science Editors:

Albin EE. Cyclic nucleotides, cyclic nucleotide phosphodiesterase, protein kinase, and phosphoprotein phosphatase in the central nervous sytem of Manduca sexta. [Doctoral Dissertation]. Oregon State University; 1973. Available from: http://hdl.handle.net/1957/45233

University of Adelaide

2. Chandler, Bruce Stanford. Spectral studies on thymine deoxyribopolynucleotides.

Degree: 1970, University of Adelaide

URL: http://hdl.handle.net/2440/20685

Subjects/Keywords: Nucleotides.

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APA (6^th Edition):

Chandler, B. S. (1970). Spectral studies on thymine deoxyribopolynucleotides. (Thesis). University of Adelaide. Retrieved from http://hdl.handle.net/2440/20685

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Chandler, Bruce Stanford. “Spectral studies on thymine deoxyribopolynucleotides.” 1970. Thesis, University of Adelaide. Accessed March 06, 2021. http://hdl.handle.net/2440/20685.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Chandler, Bruce Stanford. “Spectral studies on thymine deoxyribopolynucleotides.” 1970. Web. 06 Mar 2021.

Vancouver:

Chandler BS. Spectral studies on thymine deoxyribopolynucleotides. [Internet] [Thesis]. University of Adelaide; 1970. [cited 2021 Mar 06]. Available from: http://hdl.handle.net/2440/20685.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Chandler BS. Spectral studies on thymine deoxyribopolynucleotides. [Thesis]. University of Adelaide; 1970. Available from: http://hdl.handle.net/2440/20685

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Michigan State University

3. Marshall, Christopher Lyle. Sodium 5'-guanosine monophosphate aggregation : stoichiometry, modes of specific sodium ion complexation, and binding to ethidium.

Degree: PhD, Department of Chemistry, 1980, Michigan State University

URL: http://etd.lib.msu.edu/islandora/object/etd:41448

Subjects/Keywords: Nucleotides

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APA (6^th Edition):

Marshall, C. L. (1980). Sodium 5'-guanosine monophosphate aggregation : stoichiometry, modes of specific sodium ion complexation, and binding to ethidium. (Doctoral Dissertation). Michigan State University. Retrieved from http://etd.lib.msu.edu/islandora/object/etd:41448

Chicago Manual of Style (16^th Edition):

Marshall, Christopher Lyle. “Sodium 5'-guanosine monophosphate aggregation : stoichiometry, modes of specific sodium ion complexation, and binding to ethidium.” 1980. Doctoral Dissertation, Michigan State University. Accessed March 06, 2021. http://etd.lib.msu.edu/islandora/object/etd:41448.

MLA Handbook (7^th Edition):

Marshall, Christopher Lyle. “Sodium 5'-guanosine monophosphate aggregation : stoichiometry, modes of specific sodium ion complexation, and binding to ethidium.” 1980. Web. 06 Mar 2021.

Vancouver:

Marshall CL. Sodium 5'-guanosine monophosphate aggregation : stoichiometry, modes of specific sodium ion complexation, and binding to ethidium. [Internet] [Doctoral dissertation]. Michigan State University; 1980. [cited 2021 Mar 06]. Available from: http://etd.lib.msu.edu/islandora/object/etd:41448.

Council of Science Editors:

Marshall CL. Sodium 5'-guanosine monophosphate aggregation : stoichiometry, modes of specific sodium ion complexation, and binding to ethidium. [Doctoral Dissertation]. Michigan State University; 1980. Available from: http://etd.lib.msu.edu/islandora/object/etd:41448

4. Sun, Xuejun, 1977-. Gas phase studies of hypoxanthine:.

Degree: PhD, Chemistry and Chemical Biology, 2010, Rutgers University

URL: http://hdl.rutgers.edu/1782.2/rucore10001600001.ETD.000052158

►

Hypoxanthine is not only a naturally occurring nucleobase in tRNA but also a damaged one in DNA arising from oxidative deamination of adenine. This thesis… (more)

▼

Hypoxanthine is not only a naturally occurring nucleobase in tRNA but also a damaged one in DNA arising from oxidative deamination of adenine. This thesis describes studies of the intrinsic reactivity of hypoxanthine as a free base and the stability of DNA duplexes containing hypoxanthine in the gas phase versus in solution by calculation and mass spectrometry methods. Firstly, the free base of hypoxanthine is studied as a damaged nucleobase. Hypoxanthine is one of the damaged nucleobases that can be excised by Alkyladenine DNA glycosylase (AAG) in humans. To understand the intrinsic properties of hypoxanthine, we examined the gas phase acidity and proton affinity using quantum mechanical calculations and gas-phase mass spectrometric experimental methods. We find that the N9-H of hypoxanthine is more acidic than that of adenine and guanine, pointing to a way by which AAG may discriminate damaged bases from normal bases. We hypothesize that AAG may cleave certain damaged nucleobases as anions and the active site may take advantage of a nonpolar environment to favor deprotonated hypoxanthine as a better leaving group than adenine and guanine. The acidities of AAG substrates have been compared with those of normal bases by calculations and Cooks kinetics method. In addition, the acidity differences between damaged and normal bases are enhanced in the gas phase when we compare them with the acidities in solution. These results support our hypothesis. Secondly, to understand the effect of hypoxanthine on DNA stability, we study a series of 9-mer DNA duplexes with the sequence 5'-d(GGTTXTTGG)-3'/3'-d(CCAAYAACC)-5', where the central base X or Y = adenine (A), guanine (G), thymine (T), cytosine (C) and hypoxanthine (H). Comparison of the duplex stability in the gas phase versus in solution indicates that hypoxanthine has much less of a destabilizing effect in the gas phase versus in solution, relative to the normal complementary duplexes. The biological implications of these results are discussed in the context of hypoxanthine both as a universal base and as a damaged base.

Advisors/Committee Members: Sun, Xuejun, 1977- (author), Lee, Jeehiun (chair), Goldman, Alan (internal member), Warmuth, Ralf (internal member), Buckley, Brian (outside member).

Subjects/Keywords: Nucleotides

…long chain polymer of linked nucleotides; 5 each nucleotide is composed of a nucleobase, a…

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APA (6^th Edition):

Sun, Xuejun, 1. (2010). Gas phase studies of hypoxanthine:. (Doctoral Dissertation). Rutgers University. Retrieved from http://hdl.rutgers.edu/1782.2/rucore10001600001.ETD.000052158

Chicago Manual of Style (16^th Edition):

Sun, Xuejun, 1977-. “Gas phase studies of hypoxanthine:.” 2010. Doctoral Dissertation, Rutgers University. Accessed March 06, 2021. http://hdl.rutgers.edu/1782.2/rucore10001600001.ETD.000052158.

MLA Handbook (7^th Edition):

Sun, Xuejun, 1977-. “Gas phase studies of hypoxanthine:.” 2010. Web. 06 Mar 2021.

Vancouver:

Sun, Xuejun 1. Gas phase studies of hypoxanthine:. [Internet] [Doctoral dissertation]. Rutgers University; 2010. [cited 2021 Mar 06]. Available from: http://hdl.rutgers.edu/1782.2/rucore10001600001.ETD.000052158.

Council of Science Editors:

Sun, Xuejun 1. Gas phase studies of hypoxanthine:. [Doctoral Dissertation]. Rutgers University; 2010. Available from: http://hdl.rutgers.edu/1782.2/rucore10001600001.ETD.000052158

Hong Kong University of Science and Technology

5. Lin, Li. Nucleotides-mediated size differentiation of gold nanoparticles and its application for enzymatic assay.

Degree: 2011, Hong Kong University of Science and Technology

URL: http://repository.ust.hk/ir/Record/1783.1-7138 ; https://doi.org/10.14711/thesis-b1136621 ; http://repository.ust.hk/ir/bitstream/1783.1-7138/1/th_redirect.html

► Gold nanoparticles (Au-nps) are one of the most attractive nanomaterials used in nanotechnology and biomedical science. The wide applications of nanoparticles are highly dependent on… (more)

Subjects/Keywords: Nanoparticles – Synthesis ; Nucleotides

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APA (6^th Edition):

Lin, L. (2011). Nucleotides-mediated size differentiation of gold nanoparticles and its application for enzymatic assay. (Thesis). Hong Kong University of Science and Technology. Retrieved from http://repository.ust.hk/ir/Record/1783.1-7138 ; https://doi.org/10.14711/thesis-b1136621 ; http://repository.ust.hk/ir/bitstream/1783.1-7138/1/th_redirect.html

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Lin, Li. “Nucleotides-mediated size differentiation of gold nanoparticles and its application for enzymatic assay.” 2011. Thesis, Hong Kong University of Science and Technology. Accessed March 06, 2021. http://repository.ust.hk/ir/Record/1783.1-7138 ; https://doi.org/10.14711/thesis-b1136621 ; http://repository.ust.hk/ir/bitstream/1783.1-7138/1/th_redirect.html.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Lin, Li. “Nucleotides-mediated size differentiation of gold nanoparticles and its application for enzymatic assay.” 2011. Web. 06 Mar 2021.

Vancouver:

Lin L. Nucleotides-mediated size differentiation of gold nanoparticles and its application for enzymatic assay. [Internet] [Thesis]. Hong Kong University of Science and Technology; 2011. [cited 2021 Mar 06]. Available from: http://repository.ust.hk/ir/Record/1783.1-7138 ; https://doi.org/10.14711/thesis-b1136621 ; http://repository.ust.hk/ir/bitstream/1783.1-7138/1/th_redirect.html.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Lin L. Nucleotides-mediated size differentiation of gold nanoparticles and its application for enzymatic assay. [Thesis]. Hong Kong University of Science and Technology; 2011. Available from: http://repository.ust.hk/ir/Record/1783.1-7138 ; https://doi.org/10.14711/thesis-b1136621 ; http://repository.ust.hk/ir/bitstream/1783.1-7138/1/th_redirect.html

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Utah

6. Westcott, Wayne L. The chemistry of the nucleic acids.

Degree: MS;, Biochemistry;, 1949, University of Utah

URL: http://content.lib.utah.edu/cdm/singleitem/collection/etd2/id/198/rec/1146

► Much more is known about the components of pentose nucleic acid than is known about those of desoxypentose nucleic acid. However, the manner of the… (more)

▼ Much more is known about the components of pentose nucleic acid than is known about those of desoxypentose nucleic acid. However, the manner of the inter-nucleotide linkage of both is still unknown. An experimental approach was suggested in an attempt to settle this point for the pentose nucleic acids. Because of the extreme lability of the carbohydrate component of the desoxypentose nucleic acid a different experimental approach to the problem of inter-nucleotide linkage must be found. 2-desoxy-D-ribose has been demonstrated only in the guanosine isolated from the desoxypentose nucleic acid and its furatnose structure was demonstrated indirectly in thymidine. Infra-red studies might be carried out by determining the characteristic patters of all the known constituents of both types of the nucleic acids as well as of the nucleic acids isolated from different sources and “purified” by different methods. In this manner it may be possible to gain an insight into the over-all structures of the nucleic acid macromolecules. Studies with labeled nitrogen in the ring structure of the nitrogenous bases should be extended. The amount of each of the nitrogenous bases in the different nucleic acids could be readily determined by means of isotope dilution using the paper chromatograph for final separation. The incorporation of N15 in the ring structure of adenine at position (7) or (9) would provide a labeled nitrogenous base. This would be added to the nucleic acid before hydrolysis. The adenosine could be isolated from the hydrolysate chromatographically and the amount of adenine originally present in the nucleic acid could be determined. This procedure would have to be repeated for each nitrogenous base in order to determine the amounts originally present in the nucleic acids. Enzymatic studies also have shown the probability of more than one type of inter-nucleotide linkage occurring within the nucleic acid macromolecule. One type is thought to be quite resistant to enzymatic action as only about thirty percent of the nucleic acid is hydrolyzed under optimal conditions. It is suggested that the nucleic acids be partially hydrolyzed by the “depolymerases” and that both the hydrolyzed and non-hydrolyzed fractions be subjected to exhaustive methylation. Subsequent determinations of the carbohydrate oxidation products would aid in the location of the inter-nucleotide linkages.

Subjects/Keywords: Analysis; Nucleotides

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APA (6^th Edition):

Westcott, W. L. (1949). The chemistry of the nucleic acids. (Masters Thesis). University of Utah. Retrieved from http://content.lib.utah.edu/cdm/singleitem/collection/etd2/id/198/rec/1146

Chicago Manual of Style (16^th Edition):

Westcott, Wayne L. “The chemistry of the nucleic acids.” 1949. Masters Thesis, University of Utah. Accessed March 06, 2021. http://content.lib.utah.edu/cdm/singleitem/collection/etd2/id/198/rec/1146.

MLA Handbook (7^th Edition):

Westcott, Wayne L. “The chemistry of the nucleic acids.” 1949. Web. 06 Mar 2021.

Vancouver:

Westcott WL. The chemistry of the nucleic acids. [Internet] [Masters thesis]. University of Utah; 1949. [cited 2021 Mar 06]. Available from: http://content.lib.utah.edu/cdm/singleitem/collection/etd2/id/198/rec/1146.

Council of Science Editors:

Westcott WL. The chemistry of the nucleic acids. [Masters Thesis]. University of Utah; 1949. Available from: http://content.lib.utah.edu/cdm/singleitem/collection/etd2/id/198/rec/1146

University of Utah

7. Clemons, William Melvon. Structure of the small ribosomal subunit;.

Degree: PhD, Biochemistry;, 2000, University of Utah

URL: http://content.lib.utah.edu/cdm/singleitem/collection/etd1/id/271/rec/1255

► The ribosome is one of the most important components of the cell. It is solely responsible for protein synthesis in every organism. Aside from its… (more)

Subjects/Keywords: Genetics; Nucleotides

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APA (6^th Edition):

Clemons, W. M. (2000). Structure of the small ribosomal subunit;. (Doctoral Dissertation). University of Utah. Retrieved from http://content.lib.utah.edu/cdm/singleitem/collection/etd1/id/271/rec/1255

Chicago Manual of Style (16^th Edition):

Clemons, William Melvon. “Structure of the small ribosomal subunit;.” 2000. Doctoral Dissertation, University of Utah. Accessed March 06, 2021. http://content.lib.utah.edu/cdm/singleitem/collection/etd1/id/271/rec/1255.

MLA Handbook (7^th Edition):

Clemons, William Melvon. “Structure of the small ribosomal subunit;.” 2000. Web. 06 Mar 2021.

Vancouver:

Clemons WM. Structure of the small ribosomal subunit;. [Internet] [Doctoral dissertation]. University of Utah; 2000. [cited 2021 Mar 06]. Available from: http://content.lib.utah.edu/cdm/singleitem/collection/etd1/id/271/rec/1255.

Council of Science Editors:

Clemons WM. Structure of the small ribosomal subunit;. [Doctoral Dissertation]. University of Utah; 2000. Available from: http://content.lib.utah.edu/cdm/singleitem/collection/etd1/id/271/rec/1255

Oregon State University

8. Marchitto, Kevin Scott. The metabolism of 5'-deoxy-5'-methylthioadenosine in Saccharomyces cerevisiae.

Degree: PhD, Microbiology, 1982, Oregon State University

URL: http://hdl.handle.net/1957/41686

Subjects/Keywords: Nucleotides – Metabolism

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APA (6^th Edition):

Marchitto, K. S. (1982). The metabolism of 5'-deoxy-5'-methylthioadenosine in Saccharomyces cerevisiae. (Doctoral Dissertation). Oregon State University. Retrieved from http://hdl.handle.net/1957/41686

Chicago Manual of Style (16^th Edition):

Marchitto, Kevin Scott. “The metabolism of 5'-deoxy-5'-methylthioadenosine in Saccharomyces cerevisiae.” 1982. Doctoral Dissertation, Oregon State University. Accessed March 06, 2021. http://hdl.handle.net/1957/41686.

MLA Handbook (7^th Edition):

Marchitto, Kevin Scott. “The metabolism of 5'-deoxy-5'-methylthioadenosine in Saccharomyces cerevisiae.” 1982. Web. 06 Mar 2021.

Vancouver:

Marchitto KS. The metabolism of 5'-deoxy-5'-methylthioadenosine in Saccharomyces cerevisiae. [Internet] [Doctoral dissertation]. Oregon State University; 1982. [cited 2021 Mar 06]. Available from: http://hdl.handle.net/1957/41686.

Council of Science Editors:

Marchitto KS. The metabolism of 5'-deoxy-5'-methylthioadenosine in Saccharomyces cerevisiae. [Doctoral Dissertation]. Oregon State University; 1982. Available from: http://hdl.handle.net/1957/41686

Texas A&M University

9. Anguiano, Maritza. Effects of Dietary Nucleotides on Growth, Immunology, and Disease Resistance of Juvenile Nile Tilapia (Oreochromis niloticus).

Degree: MS, Wildlife and Fisheries Sciences, 2012, Texas A&M University

URL: http://hdl.handle.net/1969.1/ETD-TAMU-2011-12-10403

► In order to improve production efficiency and profitability in tilapia aquaculture, further research is needed to develop methods to improve weight gain, feed utilization, and… (more)

▼ In order to improve production efficiency and profitability in tilapia aquaculture, further research is needed to develop methods to improve weight gain, feed utilization, and immune function of these fish. In this regard, numerous studies with several fish species have reported that dietary nucleotides can enhance growth performance, immune responses and disease resistance. Therefore, two feeding experiments were conducted to investigate the effects of a purified nucleotide mix on juvenile Nile tilapia, Oreochromis niloticus. A basal diet was formulated to contain 34% crude protein from fishmeal and soybean meal. A nucleotide mix containing salts of cytidine, uridine, adenosine, inosine, guanosine, and thymine was supplemented to the basal diet at 0.5, 1 and 2% of dry weight. In the first experiment, three replicate groups of 15 fish were fed the experimental diets. At the end of 8 weeks, weight gain, feed efficiency and survival were computed. Blood samples were analyzed for neutrophil oxidative radical production and plasma lysozyme activity. In the second trial, three replicate groups of 20 fish were fed the same experimental diets. At the end of 4 weeks, blood and kidney samples were analyzed for macrophage extracellular and intracellular superoxide anion production, blood neutrophil oxidative radical production, plasma lysozyme activity, and peripheral blood lymphocyte proliferation. Then, 12 fish per treatment were challenged with Streptococcus iniae, via intraperitoneal injection, and mortality was recorded for 21 days. Results showed that none of the nucleotide-supplemented diets induced significant (P < 0.05) effects on growth performance. On the other hand, the 0.5% treatment produced significantly (P < 0.05) higher intracellular superoxide anion (O2-) production and both the 0.5 and 1% treatments significantly (P < 0.05) increased lymphocyte proliferation. The disease challenge failed to show significant survival differences among treatments; however, the 2% nucleotide treatment tended to produce higher survivability. Results from both experiments lead to the conclusion that this particular nucleotide mix does not provide marked improvements in growth performance and disease resistance; however, dietary nucleotide supplementation did affect some components of the immune system of Nile tilapia. Advisors/Committee Members: Buentello, Alejandro (advisor), Gatlin III, Delbert M. (advisor), Lupiani, Blanca (committee member).

Subjects/Keywords: Nile tilapia; nucleotides; Streptococcus iniae

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APA (6^th Edition):

Anguiano, M. (2012). Effects of Dietary Nucleotides on Growth, Immunology, and Disease Resistance of Juvenile Nile Tilapia (Oreochromis niloticus). (Masters Thesis). Texas A&M University. Retrieved from http://hdl.handle.net/1969.1/ETD-TAMU-2011-12-10403

Chicago Manual of Style (16^th Edition):

Anguiano, Maritza. “Effects of Dietary Nucleotides on Growth, Immunology, and Disease Resistance of Juvenile Nile Tilapia (Oreochromis niloticus).” 2012. Masters Thesis, Texas A&M University. Accessed March 06, 2021. http://hdl.handle.net/1969.1/ETD-TAMU-2011-12-10403.

MLA Handbook (7^th Edition):

Anguiano, Maritza. “Effects of Dietary Nucleotides on Growth, Immunology, and Disease Resistance of Juvenile Nile Tilapia (Oreochromis niloticus).” 2012. Web. 06 Mar 2021.

Vancouver:

Anguiano M. Effects of Dietary Nucleotides on Growth, Immunology, and Disease Resistance of Juvenile Nile Tilapia (Oreochromis niloticus). [Internet] [Masters thesis]. Texas A&M University; 2012. [cited 2021 Mar 06]. Available from: http://hdl.handle.net/1969.1/ETD-TAMU-2011-12-10403.

Council of Science Editors:

Anguiano M. Effects of Dietary Nucleotides on Growth, Immunology, and Disease Resistance of Juvenile Nile Tilapia (Oreochromis niloticus). [Masters Thesis]. Texas A&M University; 2012. Available from: http://hdl.handle.net/1969.1/ETD-TAMU-2011-12-10403

Boston College

10. Pal, Ayan. Synthesis Of Nucleoside Analogues: Glycosylation, Rigid Nucleosides And Janus Wedge Derivatives.

Degree: PhD, Chemistry, 2012, Boston College

URL: http://dlib.bc.edu/islandora/object/bc-ir:101522

► Nucleic Acids are unique biopolymers capable of encoding and transferring genetic information from one generation to the next for every form of life. This fascinating… (more)

Subjects/Keywords: Nucleic Acids; Nucleotides; Nucleotsides

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APA (6^th Edition):

Pal, A. (2012). Synthesis Of Nucleoside Analogues: Glycosylation, Rigid Nucleosides And Janus Wedge Derivatives. (Doctoral Dissertation). Boston College. Retrieved from http://dlib.bc.edu/islandora/object/bc-ir:101522

Chicago Manual of Style (16^th Edition):

Pal, Ayan. “Synthesis Of Nucleoside Analogues: Glycosylation, Rigid Nucleosides And Janus Wedge Derivatives.” 2012. Doctoral Dissertation, Boston College. Accessed March 06, 2021. http://dlib.bc.edu/islandora/object/bc-ir:101522.

MLA Handbook (7^th Edition):

Pal, Ayan. “Synthesis Of Nucleoside Analogues: Glycosylation, Rigid Nucleosides And Janus Wedge Derivatives.” 2012. Web. 06 Mar 2021.

Vancouver:

Pal A. Synthesis Of Nucleoside Analogues: Glycosylation, Rigid Nucleosides And Janus Wedge Derivatives. [Internet] [Doctoral dissertation]. Boston College; 2012. [cited 2021 Mar 06]. Available from: http://dlib.bc.edu/islandora/object/bc-ir:101522.

Council of Science Editors:

Pal A. Synthesis Of Nucleoside Analogues: Glycosylation, Rigid Nucleosides And Janus Wedge Derivatives. [Doctoral Dissertation]. Boston College; 2012. Available from: http://dlib.bc.edu/islandora/object/bc-ir:101522

University of Adelaide

11. Collins, Graham G. The effect of gibberellic acid on free nucleotide metabolism in aleurone cells of wheat / [by] Graham G. Collins.

Degree: 1970, University of Adelaide

URL: http://hdl.handle.net/2440/20708

Subjects/Keywords: Gibberellin.; Nucleotides.

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APA (6^th Edition):

Collins, G. G. (1970). The effect of gibberellic acid on free nucleotide metabolism in aleurone cells of wheat / [by] Graham G. Collins. (Thesis). University of Adelaide. Retrieved from http://hdl.handle.net/2440/20708

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Collins, Graham G. “The effect of gibberellic acid on free nucleotide metabolism in aleurone cells of wheat / [by] Graham G. Collins.” 1970. Thesis, University of Adelaide. Accessed March 06, 2021. http://hdl.handle.net/2440/20708.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Collins, Graham G. “The effect of gibberellic acid on free nucleotide metabolism in aleurone cells of wheat / [by] Graham G. Collins.” 1970. Web. 06 Mar 2021.

Vancouver:

Collins GG. The effect of gibberellic acid on free nucleotide metabolism in aleurone cells of wheat / [by] Graham G. Collins. [Internet] [Thesis]. University of Adelaide; 1970. [cited 2021 Mar 06]. Available from: http://hdl.handle.net/2440/20708.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Collins GG. The effect of gibberellic acid on free nucleotide metabolism in aleurone cells of wheat / [by] Graham G. Collins. [Thesis]. University of Adelaide; 1970. Available from: http://hdl.handle.net/2440/20708

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Adelaide

12. Grivell, Anthony Robert. Thymidine nucleotide metabolism in Neurospora crassa and other organisms : problems of specific isotype labelling of DNA in fungi.

Degree: 1970, University of Adelaide

URL: http://hdl.handle.net/2440/20816

Subjects/Keywords: Thymidine.; Nucleotides.

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APA (6^th Edition):

Grivell, A. R. (1970). Thymidine nucleotide metabolism in Neurospora crassa and other organisms : problems of specific isotype labelling of DNA in fungi. (Thesis). University of Adelaide. Retrieved from http://hdl.handle.net/2440/20816

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Grivell, Anthony Robert. “Thymidine nucleotide metabolism in Neurospora crassa and other organisms : problems of specific isotype labelling of DNA in fungi.” 1970. Thesis, University of Adelaide. Accessed March 06, 2021. http://hdl.handle.net/2440/20816.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Grivell, Anthony Robert. “Thymidine nucleotide metabolism in Neurospora crassa and other organisms : problems of specific isotype labelling of DNA in fungi.” 1970. Web. 06 Mar 2021.

Vancouver:

Grivell AR. Thymidine nucleotide metabolism in Neurospora crassa and other organisms : problems of specific isotype labelling of DNA in fungi. [Internet] [Thesis]. University of Adelaide; 1970. [cited 2021 Mar 06]. Available from: http://hdl.handle.net/2440/20816.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Grivell AR. Thymidine nucleotide metabolism in Neurospora crassa and other organisms : problems of specific isotype labelling of DNA in fungi. [Thesis]. University of Adelaide; 1970. Available from: http://hdl.handle.net/2440/20816

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Montana State University

13. Wilson, Robert Wilfred. Polarized fluorescence of the dinucleotides.

Degree: PhD, College of Letters & Science, 1974, Montana State University

URL: https://scholarworks.montana.edu/xmlui/handle/1/4671

Subjects/Keywords: Nucleotides.; Fluorescence.

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APA (6^th Edition):

Wilson, R. W. (1974). Polarized fluorescence of the dinucleotides. (Doctoral Dissertation). Montana State University. Retrieved from https://scholarworks.montana.edu/xmlui/handle/1/4671

Chicago Manual of Style (16^th Edition):

Wilson, Robert Wilfred. “Polarized fluorescence of the dinucleotides.” 1974. Doctoral Dissertation, Montana State University. Accessed March 06, 2021. https://scholarworks.montana.edu/xmlui/handle/1/4671.

MLA Handbook (7^th Edition):

Wilson, Robert Wilfred. “Polarized fluorescence of the dinucleotides.” 1974. Web. 06 Mar 2021.

Vancouver:

Wilson RW. Polarized fluorescence of the dinucleotides. [Internet] [Doctoral dissertation]. Montana State University; 1974. [cited 2021 Mar 06]. Available from: https://scholarworks.montana.edu/xmlui/handle/1/4671.

Council of Science Editors:

Wilson RW. Polarized fluorescence of the dinucleotides. [Doctoral Dissertation]. Montana State University; 1974. Available from: https://scholarworks.montana.edu/xmlui/handle/1/4671

Montana State University

14. Morgan, James Paul. Photochemistry and photophysics of guanines.

Degree: PhD, College of Letters & Science, 1974, Montana State University

URL: https://scholarworks.montana.edu/xmlui/handle/1/4458

Subjects/Keywords: Nucleotides.; Photochemistry.

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APA (6^th Edition):

Morgan, J. P. (1974). Photochemistry and photophysics of guanines. (Doctoral Dissertation). Montana State University. Retrieved from https://scholarworks.montana.edu/xmlui/handle/1/4458

Chicago Manual of Style (16^th Edition):

Morgan, James Paul. “Photochemistry and photophysics of guanines.” 1974. Doctoral Dissertation, Montana State University. Accessed March 06, 2021. https://scholarworks.montana.edu/xmlui/handle/1/4458.

MLA Handbook (7^th Edition):

Morgan, James Paul. “Photochemistry and photophysics of guanines.” 1974. Web. 06 Mar 2021.

Vancouver:

Morgan JP. Photochemistry and photophysics of guanines. [Internet] [Doctoral dissertation]. Montana State University; 1974. [cited 2021 Mar 06]. Available from: https://scholarworks.montana.edu/xmlui/handle/1/4458.

Council of Science Editors:

Morgan JP. Photochemistry and photophysics of guanines. [Doctoral Dissertation]. Montana State University; 1974. Available from: https://scholarworks.montana.edu/xmlui/handle/1/4458

University of Southern California

15. Chamberlain, Brian Thomas. Deoxynucleotide analog probes and model compounds for studying DNA polymerase structure and mechanism: synthesis and evaluation of alkyl-, azido-, and halomethylene bisphosphonate-substituted triphosphates.

Degree: PhD, Chemistry, 2012, University of Southern California

URL: http://digitallibrary.usc.edu/cdm/compoundobject/collection/p15799coll3/id/44705/rec/1840

► A variety of triphosphate analogs that replace a natural phosphate anhydride P-O-P linkage with a phosphonate P-CXY-P moiety have been synthesized for the characterization of… (more)

▼ A variety of triphosphate analogs that replace a natural phosphate anhydride P-O-P linkage with a phosphonate P-CXY-P moiety have been synthesized for the characterization of DNA polymerase β (pol β) mechanism and structure. A suite of β,γ-CXY-dTTP compounds (X,Y = H, F, Cl, Br) was synthesized to complement a previous study conducted with series of β,γ-CXY-dGTP analogs. The high purity of the β,γ-CXY-dTTP following dual-pass HPLC purification is demonstrated by NMR, MS, and HPLC data and illustrates the merit of the DCC-mediated morpholidate coupling approach to Pᵦ-CXY-Pᵧ triphosphate mimics. ❧ Novel α-azido bisphosphonates [(RO)₂P(O)]₂CXN₃ (R = i-Pr, X = Me; R = i-Pr, X = H; R = H, X = Me; and R = H, X = H) were developed to examine the impact of the unique azido steric profile on the synthesis and bioactivity of α,β-CXN₃ and β,γ-CXN₃ dNTP analogs. For one example, α,β-CMeN₃-dATP, RP HPLC separated dADP precursors that were used to generate the first examples of nucleoside triphosphate analogs with isolated stereochemistry at an asymmetrically substituted bridging carbon. The acidity constants of the α-azido bisphosphonic acids were determined by potentiometric titration and selected reactions including reduction and UV photolysis are introduced. ❧ The α,β-C(Me)₂-dATP and (R/S)-α,β-CHF-dATP analogs were synthesized and, together with the α,β-CXN₃ dATP analogs, the binding affinities (Kd) with pol β were determined. (R/S)-α,β-CHF-dATP was crystallized with a DNA-pol β binary complex. X-ray structure analysis revealed only the (S)-α,β-CFH-dATP diastereomer was present in the enzyme active site. Analysis of this structure provides evidence for a non-covalent stabilizing interaction between an active site water bound to Asp276 and the fluorine in (S)-α,β-CFH-dATP. Molecular docking studies further demonstrate the importance of this structural water by suggesting a steric clash with the methyl group of α,β-C(Me)2-dATP. ❧ A series of novel β,γ-CH₂, -CHF, and -CF₂ bisphosphonophosphate alkyl monoesters were synthesized and used as model compounds intended to estimate the activation parameters for the non-enzymatic hydrolysis of the triphosphate Pα-O-Pᵦ moiety. ¹⁸O-labeled water experiments showed that the exclusive site of nucleophilic attack in these systems is at Pᵦ. Thus, the catalytic efficiency for a small class of diphosphokinases could be approximated and the upper limit for the rate of non-enzymatic hydrolysis at Pα established. Supplementary compounds that increase Pᵦ-O bond stability by substituting Pᵧ with a phenyl ring are introduced. Preliminary hydrolysis experiments of these compounds highlight the relative stability of the Pα-O anhydride bond in triphosphates. Advisors/Committee Members: McKenna, Charles E. (Committee Chair), Goodman, Myron F. (Committee Member), Haworth, Ian S. (Committee Member).

Subjects/Keywords: bisphosphonates; organoazides; nucleotides; polymerase

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APA (6^th Edition):

Chamberlain, B. T. (2012). Deoxynucleotide analog probes and model compounds for studying DNA polymerase structure and mechanism: synthesis and evaluation of alkyl-, azido-, and halomethylene bisphosphonate-substituted triphosphates. (Doctoral Dissertation). University of Southern California. Retrieved from http://digitallibrary.usc.edu/cdm/compoundobject/collection/p15799coll3/id/44705/rec/1840

Chicago Manual of Style (16^th Edition):

Chamberlain, Brian Thomas. “Deoxynucleotide analog probes and model compounds for studying DNA polymerase structure and mechanism: synthesis and evaluation of alkyl-, azido-, and halomethylene bisphosphonate-substituted triphosphates.” 2012. Doctoral Dissertation, University of Southern California. Accessed March 06, 2021. http://digitallibrary.usc.edu/cdm/compoundobject/collection/p15799coll3/id/44705/rec/1840.

MLA Handbook (7^th Edition):

Chamberlain, Brian Thomas. “Deoxynucleotide analog probes and model compounds for studying DNA polymerase structure and mechanism: synthesis and evaluation of alkyl-, azido-, and halomethylene bisphosphonate-substituted triphosphates.” 2012. Web. 06 Mar 2021.

Vancouver:

Chamberlain BT. Deoxynucleotide analog probes and model compounds for studying DNA polymerase structure and mechanism: synthesis and evaluation of alkyl-, azido-, and halomethylene bisphosphonate-substituted triphosphates. [Internet] [Doctoral dissertation]. University of Southern California; 2012. [cited 2021 Mar 06]. Available from: http://digitallibrary.usc.edu/cdm/compoundobject/collection/p15799coll3/id/44705/rec/1840.

Council of Science Editors:

Chamberlain BT. Deoxynucleotide analog probes and model compounds for studying DNA polymerase structure and mechanism: synthesis and evaluation of alkyl-, azido-, and halomethylene bisphosphonate-substituted triphosphates. [Doctoral Dissertation]. University of Southern California; 2012. Available from: http://digitallibrary.usc.edu/cdm/compoundobject/collection/p15799coll3/id/44705/rec/1840

University of Hong Kong

16. 王勇. Evolution mechanisms and biological significance of CG dinucleotide compositional bias in genomes.

Degree: 2003, University of Hong Kong

URL: http://hdl.handle.net/10722/30444

Subjects/Keywords: Genomes.; Nucleotides.

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APA (6^th Edition):

王勇. (2003). Evolution mechanisms and biological significance of CG dinucleotide compositional bias in genomes. (Thesis). University of Hong Kong. Retrieved from http://hdl.handle.net/10722/30444

Note: this citation may be lacking information needed for this citation format:
Author name may be incomplete
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

王勇. “Evolution mechanisms and biological significance of CG dinucleotide compositional bias in genomes.” 2003. Thesis, University of Hong Kong. Accessed March 06, 2021. http://hdl.handle.net/10722/30444.

Note: this citation may be lacking information needed for this citation format:
Author name may be incomplete
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

王勇. “Evolution mechanisms and biological significance of CG dinucleotide compositional bias in genomes.” 2003. Web. 06 Mar 2021.

Note: this citation may be lacking information needed for this citation format:
Author name may be incomplete

Vancouver:

王勇. Evolution mechanisms and biological significance of CG dinucleotide compositional bias in genomes. [Internet] [Thesis]. University of Hong Kong; 2003. [cited 2021 Mar 06]. Available from: http://hdl.handle.net/10722/30444.

Note: this citation may be lacking information needed for this citation format:
Author name may be incomplete
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

王勇. Evolution mechanisms and biological significance of CG dinucleotide compositional bias in genomes. [Thesis]. University of Hong Kong; 2003. Available from: http://hdl.handle.net/10722/30444

Note: this citation may be lacking information needed for this citation format:
Author name may be incomplete
Not specified: Masters Thesis or Doctoral Dissertation

University of Georgia

17. Jung, Byoungyun. Evaluation of dietary nucleotides for broilers.

Degree: 2014, University of Georgia

URL: http://hdl.handle.net/10724/27184

► Nucleotides are the basic units that make up nucleic acids, such as deoxyribonucleic acd (DNA) and ribonucleic acid (RNA). Nucleotides are involved in many biological… (more)

▼ Nucleotides are the basic units that make up nucleic acids, such as deoxyribonucleic acd (DNA) and ribonucleic acid (RNA). Nucleotides are involved in many biological processes. They are important in nucleic acids (e.g. DNAs and RNAs) synthesis and as a source of cellular energy. They affect the immune system and are involved in physiological and morphological changes in small intestinal development. Nucleotides are not considered an essential nutrient because they can be synthesized by a de novo pathway from amino acid precursors and recycled through a salvage pathway. However, these mechanisms may not always be adequate to supply nucleotides for an organism during times of stress. Stressors which may impact nucleotide requirements include high stocking density, unsanitary conditions, disease challenges, and recovery from injuries. A series of experiments were conducted to determine the effect of the dietary nucleotides on the performance and development of the gastrointestinal tract (GIT) of broilers under certain stress conditions. In the first experiment, Torula yeast RNA (as a source of dietary nucleotide supplementation) and a commercial nucleotide product (Nupro®) were fed to broilers raised under normal conditions and environmental stressed conditions; previously used litter. Body weight gain, feed intake, feed conversion ratios, mortality, and physical and morphological development of the gastrointestinal tract were measured. Supplementing the diet with dietary nucleotides had no effect on performance of broilers under normal conditions; however, dietary nucleotide supplementation improved performance of birds reared in previously used litter. In the second experiment, the effect of Torula yeast RNA supplementation on performance, intestinal tract development and morphology of broilers challenged with a high-dose Coccivac-B® vaccine and reared in an increased stocking density were determined. Supplementing the diets with Torula yeast RNA had improved performance of birds challenged with a high-dose Coccivac-B® vaccine and reared in the increased stocking density conditions. In the third experiment, two dietary nucleotides (Torula yeast RNA and Nupro®) and a nucleotide precursor (glutamine) were fed to broilers challenged with Coccivac-B® vaccine. Broiler performance and nutrient utilization (nitrogen corrected apparent metabolizable energy; AMEN) were measured. Dietary nucleotide (0.25% Torula yeast RNA and 2% Nupro®) supplementation was effective at alleviating the negative effects of Coccidiosis vaccine challenge. The increased AMEN value of diets with supplemental levels of Torula yeast RNA and Nupro® resulted in increased growth performance. However, the supplementation of a nucleotide precursor, glutamine, did not replace nucleotides in broilers. In the fourth experiment, Torula yeast RNA was added to semi-purified diets containg high levels of casein or crystalline amino acids mixture of broilers challenged with Coccivac-B® vaccine. Performance and relative weight, morphology, and gross lesion score in…

Subjects/Keywords: Nucleotides; Performance; Stress conditions

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APA (6^th Edition):

Jung, B. (2014). Evaluation of dietary nucleotides for broilers. (Thesis). University of Georgia. Retrieved from http://hdl.handle.net/10724/27184

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Jung, Byoungyun. “Evaluation of dietary nucleotides for broilers.” 2014. Thesis, University of Georgia. Accessed March 06, 2021. http://hdl.handle.net/10724/27184.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Jung, Byoungyun. “Evaluation of dietary nucleotides for broilers.” 2014. Web. 06 Mar 2021.

Vancouver:

Jung B. Evaluation of dietary nucleotides for broilers. [Internet] [Thesis]. University of Georgia; 2014. [cited 2021 Mar 06]. Available from: http://hdl.handle.net/10724/27184.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Jung B. Evaluation of dietary nucleotides for broilers. [Thesis]. University of Georgia; 2014. Available from: http://hdl.handle.net/10724/27184

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Michigan State University

18. Dannert, Robert Dale. The determination of 5'-mononucleotides in meats.

Degree: PhD, Department of Food Science, 1966, Michigan State University

URL: http://etd.lib.msu.edu/islandora/object/etd:17702

Subjects/Keywords: Nucleotides; Meat

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APA (6^th Edition):

Dannert, R. D. (1966). The determination of 5'-mononucleotides in meats. (Doctoral Dissertation). Michigan State University. Retrieved from http://etd.lib.msu.edu/islandora/object/etd:17702

Chicago Manual of Style (16^th Edition):

Dannert, Robert Dale. “The determination of 5'-mononucleotides in meats.” 1966. Doctoral Dissertation, Michigan State University. Accessed March 06, 2021. http://etd.lib.msu.edu/islandora/object/etd:17702.

MLA Handbook (7^th Edition):

Dannert, Robert Dale. “The determination of 5'-mononucleotides in meats.” 1966. Web. 06 Mar 2021.

Vancouver:

Dannert RD. The determination of 5'-mononucleotides in meats. [Internet] [Doctoral dissertation]. Michigan State University; 1966. [cited 2021 Mar 06]. Available from: http://etd.lib.msu.edu/islandora/object/etd:17702.

Council of Science Editors:

Dannert RD. The determination of 5'-mononucleotides in meats. [Doctoral Dissertation]. Michigan State University; 1966. Available from: http://etd.lib.msu.edu/islandora/object/etd:17702

Brigham Young University

19. Rowe, Mark J. Enzymatic synthesis of oligoribonucleotides of defined base sequence.

Degree: PhD, 1972, Brigham Young University

URL: https://scholarsarchive.byu.edu/cgi/viewcontent.cgi?article=9344&context=etd

► The synthetic reaction of ribonuclease T_1 has been studied as to its potential for the synthesis of oligoribonucleotides of defined base sequence. Conditions have… (more)

Subjects/Keywords: Nucleotides; Chemistry

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APA (6^th Edition):

Rowe, M. J. (1972). Enzymatic synthesis of oligoribonucleotides of defined base sequence. (Doctoral Dissertation). Brigham Young University. Retrieved from https://scholarsarchive.byu.edu/cgi/viewcontent.cgi?article=9344&context=etd

Chicago Manual of Style (16^th Edition):

Rowe, Mark J. “Enzymatic synthesis of oligoribonucleotides of defined base sequence.” 1972. Doctoral Dissertation, Brigham Young University. Accessed March 06, 2021. https://scholarsarchive.byu.edu/cgi/viewcontent.cgi?article=9344&context=etd.

MLA Handbook (7^th Edition):

Rowe, Mark J. “Enzymatic synthesis of oligoribonucleotides of defined base sequence.” 1972. Web. 06 Mar 2021.

Vancouver:

Rowe MJ. Enzymatic synthesis of oligoribonucleotides of defined base sequence. [Internet] [Doctoral dissertation]. Brigham Young University; 1972. [cited 2021 Mar 06]. Available from: https://scholarsarchive.byu.edu/cgi/viewcontent.cgi?article=9344&context=etd.

Council of Science Editors:

Rowe MJ. Enzymatic synthesis of oligoribonucleotides of defined base sequence. [Doctoral Dissertation]. Brigham Young University; 1972. Available from: https://scholarsarchive.byu.edu/cgi/viewcontent.cgi?article=9344&context=etd

The Ohio State University

20. Pagnucco, Rinaldo Gene. Synthesis of purine nucleosides of D-galactose and D-galacturonic acid derivatives.

Degree: PhD, Graduate School, 1965, The Ohio State University

URL: http://rave.ohiolink.edu/etdc/view?acc_num=osu1486567958464259

Subjects/Keywords: Chemistry; Nucleotides

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APA (6^th Edition):

Pagnucco, R. G. (1965). Synthesis of purine nucleosides of D-galactose and D-galacturonic acid derivatives. (Doctoral Dissertation). The Ohio State University. Retrieved from http://rave.ohiolink.edu/etdc/view?acc_num=osu1486567958464259

Chicago Manual of Style (16^th Edition):

Pagnucco, Rinaldo Gene. “Synthesis of purine nucleosides of D-galactose and D-galacturonic acid derivatives.” 1965. Doctoral Dissertation, The Ohio State University. Accessed March 06, 2021. http://rave.ohiolink.edu/etdc/view?acc_num=osu1486567958464259.

MLA Handbook (7^th Edition):

Pagnucco, Rinaldo Gene. “Synthesis of purine nucleosides of D-galactose and D-galacturonic acid derivatives.” 1965. Web. 06 Mar 2021.

Vancouver:

Pagnucco RG. Synthesis of purine nucleosides of D-galactose and D-galacturonic acid derivatives. [Internet] [Doctoral dissertation]. The Ohio State University; 1965. [cited 2021 Mar 06]. Available from: http://rave.ohiolink.edu/etdc/view?acc_num=osu1486567958464259.

Council of Science Editors:

Pagnucco RG. Synthesis of purine nucleosides of D-galactose and D-galacturonic acid derivatives. [Doctoral Dissertation]. The Ohio State University; 1965. Available from: http://rave.ohiolink.edu/etdc/view?acc_num=osu1486567958464259

Rutgers University

21. Hicks, Allison, 1988-. Cell tropism predicts long-term nucleotide substitution rates of mammalian RNA viruses.

Degree: MS, Microbial Biology, 2013, Rutgers University

URL: https://rucore.libraries.rutgers.edu/rutgers-lib/41786/

► The high rates of RNA virus evolution are generally attributed to replication with error-prone RNA-dependent RNA polymerases. However, these long-term nucleotide substitution rates span three… (more)

▼ The high rates of RNA virus evolution are generally attributed to replication with error-prone RNA-dependent RNA polymerases. However, these long-term nucleotide substitution rates span three orders of magnitude and do not correlate well with mutation rates or selection pressures. This substitution rate variation may be explained by differences in virus ecology or intrinsic genomic properties. We generated long-term nucleotide substitution rate estimates for mammalian RNA viruses and compiled comparable published rates, yielding a dataset of 118 substitution rates of structural genes from 51 different species, as well as 40 rates of non-structural genes from 28 species. Through multiple regression analyses, we evaluated the relationships between these rates and four ecological factors: target cell, transmission route, host range, infection duration; and three genomic properties: genome length, genome sense, genome segmentation. Of these seven factors, we found target cells to be the only significant predictors of viral substitution rates, with tropisms for epithelial cells (P<2x10-5 for the structural genes) or neurons (P<3x10-7 and P<0.01 for the structural genes and non-structural genes, respectively) as the most significant predictors. Further, one-tailed t-tests showed that viruses primarily infecting epithelial cells evolve significantly faster than neurotropic viruses (P=1.83x10-10 and P=6.30x10-4 for the structural genes and non-structural genes, respectively). These results provide strong evidence that the fastest evolving mammalian RNA viruses infect cells with the highest turnover rates: the highly proliferative epithelial cells. Estimated viral generation times suggest that epithelial-infecting viruses replicate more quickly than viruses with different cell tropisms. Our results indicate that cell tropism is a key factor in viral evolvability. Advisors/Committee Members: Duffy, Siobain (chair), Hillman, Bradley (internal member), Kahn, Peter C. (internal member).

Subjects/Keywords: RNA viruses; Nucleotides; Mammals – Cytology

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APA (6^th Edition):

Hicks, Allison, 1. (2013). Cell tropism predicts long-term nucleotide substitution rates of mammalian RNA viruses. (Masters Thesis). Rutgers University. Retrieved from https://rucore.libraries.rutgers.edu/rutgers-lib/41786/

Chicago Manual of Style (16^th Edition):

Hicks, Allison, 1988-. “Cell tropism predicts long-term nucleotide substitution rates of mammalian RNA viruses.” 2013. Masters Thesis, Rutgers University. Accessed March 06, 2021. https://rucore.libraries.rutgers.edu/rutgers-lib/41786/.

MLA Handbook (7^th Edition):

Hicks, Allison, 1988-. “Cell tropism predicts long-term nucleotide substitution rates of mammalian RNA viruses.” 2013. Web. 06 Mar 2021.

Vancouver:

Hicks, Allison 1. Cell tropism predicts long-term nucleotide substitution rates of mammalian RNA viruses. [Internet] [Masters thesis]. Rutgers University; 2013. [cited 2021 Mar 06]. Available from: https://rucore.libraries.rutgers.edu/rutgers-lib/41786/.

Council of Science Editors:

Hicks, Allison 1. Cell tropism predicts long-term nucleotide substitution rates of mammalian RNA viruses. [Masters Thesis]. Rutgers University; 2013. Available from: https://rucore.libraries.rutgers.edu/rutgers-lib/41786/

Michigan State University

22. Kroon, Daniel John, 1949-. Analogs of nicotinamide adenine dinucleotide as probes of the stereochemical relationships in the ternary complex of alcohol dehydrogenases.

Degree: PhD, 1976, Michigan State University

URL: http://etd.lib.msu.edu/islandora/object/etd:34989

Subjects/Keywords: Nucleotides; Nucleotides – Metabolism; Dehydrogenases; Stereochemistry

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APA (6^th Edition):

Kroon, Daniel John, 1. (1976). Analogs of nicotinamide adenine dinucleotide as probes of the stereochemical relationships in the ternary complex of alcohol dehydrogenases. (Doctoral Dissertation). Michigan State University. Retrieved from http://etd.lib.msu.edu/islandora/object/etd:34989

Chicago Manual of Style (16^th Edition):

Kroon, Daniel John, 1949-. “Analogs of nicotinamide adenine dinucleotide as probes of the stereochemical relationships in the ternary complex of alcohol dehydrogenases.” 1976. Doctoral Dissertation, Michigan State University. Accessed March 06, 2021. http://etd.lib.msu.edu/islandora/object/etd:34989.

MLA Handbook (7^th Edition):

Kroon, Daniel John, 1949-. “Analogs of nicotinamide adenine dinucleotide as probes of the stereochemical relationships in the ternary complex of alcohol dehydrogenases.” 1976. Web. 06 Mar 2021.

Vancouver:

Kroon, Daniel John 1. Analogs of nicotinamide adenine dinucleotide as probes of the stereochemical relationships in the ternary complex of alcohol dehydrogenases. [Internet] [Doctoral dissertation]. Michigan State University; 1976. [cited 2021 Mar 06]. Available from: http://etd.lib.msu.edu/islandora/object/etd:34989.

Council of Science Editors:

Kroon, Daniel John 1. Analogs of nicotinamide adenine dinucleotide as probes of the stereochemical relationships in the ternary complex of alcohol dehydrogenases. [Doctoral Dissertation]. Michigan State University; 1976. Available from: http://etd.lib.msu.edu/islandora/object/etd:34989

Dalhousie University

23. Beaton, Stephen A. Evaluation and Synthesis of Sugar 1-Phosphate Substrates for Nucleotidylyltransferases.

Degree: MS, Department of Chemistry, 2010, Dalhousie University

URL: http://hdl.handle.net/10222/12815

► The study of many of glycosyltransferases is limited due to an inadequate access to sugar nucleotides. Preparation of sugar nucleotides through the use of nucleotidylyltransferases… (more)

Subjects/Keywords: Nucleotidylyltransferases; organic synthesis; enzymes; sugar nucleotides

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APA (6^th Edition):

Beaton, S. A. (2010). Evaluation and Synthesis of Sugar 1-Phosphate Substrates for Nucleotidylyltransferases. (Masters Thesis). Dalhousie University. Retrieved from http://hdl.handle.net/10222/12815

Chicago Manual of Style (16^th Edition):

Beaton, Stephen A. “Evaluation and Synthesis of Sugar 1-Phosphate Substrates for Nucleotidylyltransferases.” 2010. Masters Thesis, Dalhousie University. Accessed March 06, 2021. http://hdl.handle.net/10222/12815.

MLA Handbook (7^th Edition):

Beaton, Stephen A. “Evaluation and Synthesis of Sugar 1-Phosphate Substrates for Nucleotidylyltransferases.” 2010. Web. 06 Mar 2021.

Vancouver:

Beaton SA. Evaluation and Synthesis of Sugar 1-Phosphate Substrates for Nucleotidylyltransferases. [Internet] [Masters thesis]. Dalhousie University; 2010. [cited 2021 Mar 06]. Available from: http://hdl.handle.net/10222/12815.

Council of Science Editors:

Beaton SA. Evaluation and Synthesis of Sugar 1-Phosphate Substrates for Nucleotidylyltransferases. [Masters Thesis]. Dalhousie University; 2010. Available from: http://hdl.handle.net/10222/12815

Queensland University of Technology

24. Narayan, Vicrant S. HPLC analysis of nucleotides.

Degree: 2017, Queensland University of Technology

URL: https://eprints.qut.edu.au/104440/

► This project developed a novel hydrophilic interaction chromatography method for the detection and identification of nucleotides. The method was validated by testing biological specimens; adult… (more)

Subjects/Keywords: HILIC; Saliva; HPLC-UV; Nucleotides; HILIC-UV

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APA (6^th Edition):

Narayan, V. S. (2017). HPLC analysis of nucleotides. (Thesis). Queensland University of Technology. Retrieved from https://eprints.qut.edu.au/104440/

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Narayan, Vicrant S. “HPLC analysis of nucleotides.” 2017. Thesis, Queensland University of Technology. Accessed March 06, 2021. https://eprints.qut.edu.au/104440/.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Narayan, Vicrant S. “HPLC analysis of nucleotides.” 2017. Web. 06 Mar 2021.

Vancouver:

Narayan VS. HPLC analysis of nucleotides. [Internet] [Thesis]. Queensland University of Technology; 2017. [cited 2021 Mar 06]. Available from: https://eprints.qut.edu.au/104440/.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Narayan VS. HPLC analysis of nucleotides. [Thesis]. Queensland University of Technology; 2017. Available from: https://eprints.qut.edu.au/104440/

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Penn State University

25. Kehoe, Sylvia Inga. COLOSTRUM COMPONENTS AND THEIR IMPACT ON DIGESTIVE FUNCTION AND GROWTH OF DAIRY CALVES .

Degree: 2008, Penn State University

URL: https://submit-etda.libraries.psu.edu/catalog/7379

► Colostrum is the first important step in raising healthy dairy calves. Feeding of adequate volumes of colostrum within the first 2 hours after birth improves… (more)

▼ Colostrum is the first important step in raising healthy dairy calves. Feeding of adequate volumes of colostrum within the first 2 hours after birth improves calf health, immunity and productive lifespan. Two experiments were conducted, the first was to evaluate and record colostrum management and nutrient composition to determine any improved or deleterious changes. The second was to evaluate the effects of a particular component of colostrum, nucleotides, on enhancing absorptive capacity and decreasing diarrhea in neonatal dairy calves. For the first experiment, data and samples were obtained from 55 farms around the state of Pennsylvania. Samples were analyzed for fat, protein, lactose, total solids, ash, immunoglobulins, lactoferrin, water- and fat-soluble vitamins and minerals. Results of means of fat, protein, lactose and lactoferrin were as follows: 6.7%, 14.92%, 2.49% and 0.82 mg/ml, respectively. Immunoglobulin G1, G2, A and M were 35.1 ± 11.9, 5.6 ± 1.9, 1.7 ± 0.9 and 4.3 ± 2.8 g/L, respectively. Fat-soluble vitamins included retinol, tocopherol and B-carotene and were 4.9, 2.92 and 0.68 ug/g, respectively. Means of water-soluble vitamins were 0.34, 0.90, 4.55, 0.60, 0.15, 0.21 and 0.04 for niacinamide, thiamine, riboflavin, cyanocobalamin, pyridoxal, pyridoxamine and pyridoxine, respectively. Means of minerals (mg/kg) consisted of calcium 4716.08; phosphorus 4452.12; magnesium 733.24; sodium 1058.93; potassium 2845.89; zinc 38.10; iron 5.33; copper 0.34 and manganese 0.10. In comparison to previous reviews of bovine colostrum, most nutrient levels have increased. Management practices have also improved in areas of colostrum storage and feeding. Calves were fed by bottle in 85% of farms and only 1 farm allowed calves to nurse the dam. Calves were fed first colostrum within 6 hours of birth in 95% of farms and 38% of farms had a supply of frozen colostrum readily available for use. For the second experiment, to analyze for effects of feeding purified or yeast derived nucleotides, calves were fed milk replacer at 10% of BW daily and treatments consisted of no nucleotide supplementation (C), purified nucleotide supplementation (N) and yeast supplementation (P; Alltech, Inc.). Growth, health, fecal dry matters and fecal bacteria were monitored and blood was analyzed for hematocrits, glucose, blood urea nitrogen and creatinine. After the second day of scouring, xylose (0.5 g/kg of BW) was administered orally once and blood was taken before administration for a baseline as well as 1, 2, 3 and 4 h post-dosing. Concentrations of plasma xylose increased with time post-dosing, but did not vary by treatment. Hematocrits were significantly higher for treatment N for weeks 2 and 5. Growth parameters and plasma concentrations of glucose, BUN, creatinine and IgG were not different between treatments. There were also no treatment effects on fecal dry matters and concentrations of fecal bacteria. Intestinal parameters, including morphology, enzyme activities, DNA and protein concentrations were… Advisors/Committee Members: Arlyn Judson Heinrichs, Committee Chair/Co-Chair, Craig Richard Baumrucker, Committee Member, Chad Daniel Dechow, Committee Member, Bhushan M Jayarao, Committee Member, Gabriella Anne Varga, Committee Member.

Subjects/Keywords: nucleotides; calves; colostrum

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APA (6^th Edition):

Kehoe, S. I. (2008). COLOSTRUM COMPONENTS AND THEIR IMPACT ON DIGESTIVE FUNCTION AND GROWTH OF DAIRY CALVES . (Thesis). Penn State University. Retrieved from https://submit-etda.libraries.psu.edu/catalog/7379

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Kehoe, Sylvia Inga. “COLOSTRUM COMPONENTS AND THEIR IMPACT ON DIGESTIVE FUNCTION AND GROWTH OF DAIRY CALVES .” 2008. Thesis, Penn State University. Accessed March 06, 2021. https://submit-etda.libraries.psu.edu/catalog/7379.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Kehoe, Sylvia Inga. “COLOSTRUM COMPONENTS AND THEIR IMPACT ON DIGESTIVE FUNCTION AND GROWTH OF DAIRY CALVES .” 2008. Web. 06 Mar 2021.

Vancouver:

Kehoe SI. COLOSTRUM COMPONENTS AND THEIR IMPACT ON DIGESTIVE FUNCTION AND GROWTH OF DAIRY CALVES . [Internet] [Thesis]. Penn State University; 2008. [cited 2021 Mar 06]. Available from: https://submit-etda.libraries.psu.edu/catalog/7379.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Kehoe SI. COLOSTRUM COMPONENTS AND THEIR IMPACT ON DIGESTIVE FUNCTION AND GROWTH OF DAIRY CALVES . [Thesis]. Penn State University; 2008. Available from: https://submit-etda.libraries.psu.edu/catalog/7379

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Delaware

26. Lertsuwan, Jomnarong. Effects of adenine nucleotide agents and their therapeutic potential on triple negative breast cancer.

Degree: PhD, University of Delaware, Department of Biological Sciences, 2014, University of Delaware

URL: http://udspace.udel.edu/handle/19716/16758

► Breast cancer (BCa) is the most frequently diagnosed non-cutaneous cancer in the United States and the second leading cause of cancer death of American women.… (more)

▼ Breast cancer (BCa) is the most frequently diagnosed non-cutaneous cancer in the United States and the second leading cause of cancer death of American women. Over the last several decades, enhancement of BCa screening and early detection improved patients survival rate and patients' well-being. However, some aggressive types of BCa still threaten BCa patients' well- being. Triple negative breast cancer (TNBC) is one of the most aggressive types of breast cancer and needs new promising therapeutic agents due to its lack in responsiveness to hormonal therapies or HER2 targeting drugs due to the absence of progesterone receptor, estrogen receptor, and HER2 receptor. Therefore, developing novel therapeutic agents and/or strategies aimed to prevent TNBC growth and to attenuate its behavior associated with metastasis will at improve patients' well-being ultimately cure the disease. ATP was considered originally as only an intracellular energy source. The concept that ATP is an extracellular messenger was not widely accepted until its receptors was cloned in early 1990s. Subsequently, purinergic signaling was investigated and its association with cell growth and proliferation was discovered. One of ATP analogs, 2 feet, 3 feet-O-(4-benzoylbenzoyl)-ATP (BzATP) showed wide range of both in vitro and in vivo tumor suppression such as cell proliferation inhibition and apoptosis induction. The inhibitory effects on cell proliferation, cell migration, and cell invasion of adenine nucleotides and other adenine agents on BCa were observed in vitro. Colony formation assay revealed a suppression of adenine nucleotides especially BzATP on ability of TNBC cells to form colonies. The pilot in vivo experiment demonstrated tumor growth inhibition effect of BzATP in vivo. These in vivo data confirmed an inhibitory effect of BzATP on TNBC tumor growth in vitro. P2Y11 was identified as a receptor mediating inhibitory effect of BzATP. Further analysis on cell death mechanism revealed that BzATP induced ER stress, autophagy and apoptosis, but not necrosis, through P2Y11 receptor. Unlike autophagy reported in many literatures that it is survival pathway cancer cells exploited to avoid apoptosis, BzATP-induced autophagy may not be a survival mechanism. Remarkably, BzATP did not induce apoptosis in non-cancerous mammary epithelial cells, MCF10A. Advisors/Committee Members: Sikes, Robert A..

Subjects/Keywords: Adenine nucleotides.; Breast – Cancer.; Adenosine triphosphate.

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Lertsuwan, J. (2014). Effects of adenine nucleotide agents and their therapeutic potential on triple negative breast cancer. (Doctoral Dissertation). University of Delaware. Retrieved from http://udspace.udel.edu/handle/19716/16758

Chicago Manual of Style (16^th Edition):

Lertsuwan, Jomnarong. “Effects of adenine nucleotide agents and their therapeutic potential on triple negative breast cancer.” 2014. Doctoral Dissertation, University of Delaware. Accessed March 06, 2021. http://udspace.udel.edu/handle/19716/16758.

MLA Handbook (7^th Edition):

Lertsuwan, Jomnarong. “Effects of adenine nucleotide agents and their therapeutic potential on triple negative breast cancer.” 2014. Web. 06 Mar 2021.

Vancouver:

Lertsuwan J. Effects of adenine nucleotide agents and their therapeutic potential on triple negative breast cancer. [Internet] [Doctoral dissertation]. University of Delaware; 2014. [cited 2021 Mar 06]. Available from: http://udspace.udel.edu/handle/19716/16758.

Council of Science Editors:

Lertsuwan J. Effects of adenine nucleotide agents and their therapeutic potential on triple negative breast cancer. [Doctoral Dissertation]. University of Delaware; 2014. Available from: http://udspace.udel.edu/handle/19716/16758

University College London (University of London)

27. Hoebertz, Astrid. Purinergic signalling in bone cells.

Degree: PhD, 2001, University College London (University of London)

URL: https://discovery.ucl.ac.uk/id/eprint/10098451/ ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.249706

► There is increasing evidence that ATP and other extracellular nucleotides, signalling through P2 receptors, play an important role in bone remodelling. I used immunohistochemistry and… (more)

▼ There is increasing evidence that ATP and other extracellular nucleotides, signalling through P2 receptors, play an important role in bone remodelling. I used immunohistochemistry and in situ hybridisation to study the expression of P2 receptors in rat bone sections and on cultured bone cells: osteoclasts, the multinucleated cells responsible for bone resorption, osteoblasts, the bone-forming cells, and chondrocytes were all shown to express a number of P2 receptors, both of the ionotropic P2X family and the metabotropic P2Y family. ATP has previously been shown to be a potent stimulator of bone resorption, however, it was not known which receptor subtypes mediate this stimulatory effect. Three different models were used to study the effects of a wider range of P2 receptor agonists on osteoclast recruitment and resorption: the 26 hour disaggregated rat osteoclast assay, 72 hour whole organ mouse calvaria cultures, and 10 day mouse marrow cultures. My main discovery is that extracellular ADP, the first degradation product of ATP, is a powerful stimulator, at nanomolar concentrations, of osteoclast activation and recruitment. Furthermore, evidence is provided, using subtype-selective agonists and antagonists, that these ADP effects are probably mediated via the P2Y1 receptor. It is suggested that in vivo, ADP could be involved in the mediation of inflammatory bone loss. The effects of nucleotides on osteoblast function were studied using the bone nodule assay by culturing primary rat calvarial osteoblasts for 3 weeks and by analysing the formation of mineralised bone nodules. Both ATP and UTP, a P2Y2 and P2Y4 receptor agonist, significantly inhibited bone nodule number at concentrations 0.1-10μM, whereas ADP showed no effect. Taken together, these results suggest subtype-specific roles for P2 receptors present in bone cells: ADP is a powerful stimulator of bone resorption signalling via the P2Y1 receptor, whereas UTP, signalling via the P2Y2 receptor, could play a role as an inhibitor of bone formation. In parallel, further functional experiments were carried out to assess the interactions of low pH and of osteolytic stimuli, and to investigate whether human osteoclasts are as acid-sensitive as rodent and avian osteoclasts. Interestingly, both nucleotides (ADP and ATP) and the cytokine RANKL require low pH to show their full stimulatory effect on bone resorption, suggesting that low pH is a key requirement for resorption to occur. In conclusion, this is one of the first studies linking specific P2 receptor subtypes to key functional actions of extracellular nucleotides on bone.

Subjects/Keywords: 610; Extracellular nucleotides

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APA (6^th Edition):

Hoebertz, A. (2001). Purinergic signalling in bone cells. (Doctoral Dissertation). University College London (University of London). Retrieved from https://discovery.ucl.ac.uk/id/eprint/10098451/ ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.249706

Chicago Manual of Style (16^th Edition):

Hoebertz, Astrid. “Purinergic signalling in bone cells.” 2001. Doctoral Dissertation, University College London (University of London). Accessed March 06, 2021. https://discovery.ucl.ac.uk/id/eprint/10098451/ ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.249706.

MLA Handbook (7^th Edition):

Hoebertz, Astrid. “Purinergic signalling in bone cells.” 2001. Web. 06 Mar 2021.

Vancouver:

Hoebertz A. Purinergic signalling in bone cells. [Internet] [Doctoral dissertation]. University College London (University of London); 2001. [cited 2021 Mar 06]. Available from: https://discovery.ucl.ac.uk/id/eprint/10098451/ ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.249706.

Council of Science Editors:

Hoebertz A. Purinergic signalling in bone cells. [Doctoral Dissertation]. University College London (University of London); 2001. Available from: https://discovery.ucl.ac.uk/id/eprint/10098451/ ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.249706

Boston College

28. Theile, Christopher Stone. Synthesis of Cyclo, Ring Expanded, and Backbone Extended Nucleosides.

Degree: PhD, Chemistry, 2012, Boston College

URL: http://dlib.bc.edu/islandora/object/bc-ir:101931

► Nucleic acids are responsible for maintaining the biological information responsible for the activities of all known living organisms. Research of nucleic acids provides opportunities to… (more)

Subjects/Keywords: cyclonucleoside; DNA; Nucleic Acids; Nucleosides; nucleotides; RNA

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APA (6^th Edition):

Theile, C. S. (2012). Synthesis of Cyclo, Ring Expanded, and Backbone Extended Nucleosides. (Doctoral Dissertation). Boston College. Retrieved from http://dlib.bc.edu/islandora/object/bc-ir:101931

Chicago Manual of Style (16^th Edition):

Theile, Christopher Stone. “Synthesis of Cyclo, Ring Expanded, and Backbone Extended Nucleosides.” 2012. Doctoral Dissertation, Boston College. Accessed March 06, 2021. http://dlib.bc.edu/islandora/object/bc-ir:101931.

MLA Handbook (7^th Edition):

Theile, Christopher Stone. “Synthesis of Cyclo, Ring Expanded, and Backbone Extended Nucleosides.” 2012. Web. 06 Mar 2021.

Vancouver:

Theile CS. Synthesis of Cyclo, Ring Expanded, and Backbone Extended Nucleosides. [Internet] [Doctoral dissertation]. Boston College; 2012. [cited 2021 Mar 06]. Available from: http://dlib.bc.edu/islandora/object/bc-ir:101931.

Council of Science Editors:

Theile CS. Synthesis of Cyclo, Ring Expanded, and Backbone Extended Nucleosides. [Doctoral Dissertation]. Boston College; 2012. Available from: http://dlib.bc.edu/islandora/object/bc-ir:101931

Hong Kong University of Science and Technology

29. Zhao, Wenting. A nucleotide-mediated strategy for the synthesis of bio-functionalized gold-nanoparticles.

Degree: 2010, Hong Kong University of Science and Technology

URL: http://repository.ust.hk/ir/Record/1783.1-7001 ; https://doi.org/10.14711/thesis-b1114999 ; http://repository.ust.hk/ir/bitstream/1783.1-7001/1/th_redirect.html

► Bio-functionalized gold nanoparticles (Au-nps) are among the most attractive nanomaterials and are widely used in nanotechnology and biomedical science. They were intensively studied as versatile… (more)

▼ Bio-functionalized gold nanoparticles (Au-nps) are among the most attractive nanomaterials and are widely used in nanotechnology and biomedical science. They were intensively studied as versatile reporters and carriers in developing molecular diagnostic methods, regulating intracellular gene expression, as well as building blocks for various nanostructures. One of the fundamental necessary abilities for implementing the above applications is to efficiently conjugate biomolecules to Au-nps without sacrificing either the stability of the nanoparticles or the functionality of the biomolecules. In this thesis work, we developed a novel nucleotide-mediated strategy for bio-functionalization of Au-nps that combines rapid Au-nps stabilization with facile biomolecular conjugation. Mononucleotides were found to form an adsorption layer on the Au-nps surface in a few minutes, thus stabilizing the Au-nps against salt-induced aggregation. This layer is thermally detachable, enabling its fast replacement by thiolated biomolecules. This newly developed bio-functionalization method therefore enables functionalization of the Au-nps in salt environment, unlike previous methods, with an overall synthesis time shortened significantly from hours and days to minutes without compromising the biomolecular loading on the Au-nps. Further surface density control was achieved by tuning the conjugation speed through salt concentration and by defining the conjugation time through stopping reagent introduced in a synergistic fashion. As a more advanced step towards applications, peptides were also conjugated to the Au-nps using a similar approach. Various strategies for DNA and peptide multi-functionalization on Au-nps were further investigated. The versatile function of DNA and peptide co-conjugated Au-nps was briefly demonstrated by multiplex detection of nuclease and protease using nanoparticle-based gel electrophoresis. The developed nucleotide-mediated functionalization strategy offers an attractive alternative for the preparation of bio-functionalized Au-nps. The ability to modify Au-nps with various DNA and peptide strands in a rapid, controlled and versatile manner opens up new exciting opportunities for nano- and bio- applications.

Subjects/Keywords: Nanoparticles – Synthesis ; Nucleotides

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APA (6^th Edition):

Zhao, W. (2010). A nucleotide-mediated strategy for the synthesis of bio-functionalized gold-nanoparticles. (Thesis). Hong Kong University of Science and Technology. Retrieved from http://repository.ust.hk/ir/Record/1783.1-7001 ; https://doi.org/10.14711/thesis-b1114999 ; http://repository.ust.hk/ir/bitstream/1783.1-7001/1/th_redirect.html

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Zhao, Wenting. “A nucleotide-mediated strategy for the synthesis of bio-functionalized gold-nanoparticles.” 2010. Thesis, Hong Kong University of Science and Technology. Accessed March 06, 2021. http://repository.ust.hk/ir/Record/1783.1-7001 ; https://doi.org/10.14711/thesis-b1114999 ; http://repository.ust.hk/ir/bitstream/1783.1-7001/1/th_redirect.html.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Zhao, Wenting. “A nucleotide-mediated strategy for the synthesis of bio-functionalized gold-nanoparticles.” 2010. Web. 06 Mar 2021.

Vancouver:

Zhao W. A nucleotide-mediated strategy for the synthesis of bio-functionalized gold-nanoparticles. [Internet] [Thesis]. Hong Kong University of Science and Technology; 2010. [cited 2021 Mar 06]. Available from: http://repository.ust.hk/ir/Record/1783.1-7001 ; https://doi.org/10.14711/thesis-b1114999 ; http://repository.ust.hk/ir/bitstream/1783.1-7001/1/th_redirect.html.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Zhao W. A nucleotide-mediated strategy for the synthesis of bio-functionalized gold-nanoparticles. [Thesis]. Hong Kong University of Science and Technology; 2010. Available from: http://repository.ust.hk/ir/Record/1783.1-7001 ; https://doi.org/10.14711/thesis-b1114999 ; http://repository.ust.hk/ir/bitstream/1783.1-7001/1/th_redirect.html

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Tartu University

30. Alves Oliveira, Sofia Raquel. HPLC analysis of bacterial alarmone nucleotide (p)ppGpp and its toxic analogue ppApp .

Degree: 2020, Tartu University

URL: http://hdl.handle.net/10062/69497

► Bakteritel on evolutsiooni käigus välja kujunenud arvukalt kohanemismehhanisme, mis aitavad neil ellu jääda ka karmides keskkonnatingimustes. Keerukad molekulaarsed võrgustikud kontrollivad adaptiivseid füsioloogilisi vastuseid, näiteks antibiootikumiresistentsust,… (more)

▼ Bakteritel on evolutsiooni käigus välja kujunenud arvukalt kohanemismehhanisme, mis aitavad neil ellu jääda ka karmides keskkonnatingimustes. Keerukad molekulaarsed võrgustikud kontrollivad adaptiivseid füsioloogilisi vastuseid, näiteks antibiootikumiresistentsust, biokile moodustumist ja bakterite minekut uinunud olekusse. Sellised kohanemismehhanismid sõltuvad stressi tajuvate ja sellele reageerivate valkude ensümaatilistest aktiivsustest. Üheks oluliseks komponendiks stressivastuses on signaalmolekulide süntees ja lagundamine. Käesolevas töös uuriti ühte kõige laiemalt levinud adaptiivset mehhanismi, mida nimetatakse poomisvastuseks. Selle mehhanismi puhul on võtmetähtsusega RelA / SpoT homoloogsed (RSH) ensüümid, mis sünteesivad ja lagundavad alarmoon-nukleotiide ppGpp ja ppp(G)pp. Nende nukleotiidide ühiseks nimetamiseks kasutatakse tähistust (p)ppGpp. Need molekulid mõjutavad mitmeid protsesse bakterirakus, näiteks virulentsust ja antibiootikumitolerantsust. Käesoleva töö eesmärgiks oli välja töötada metoodika nukleotiidide, sealhulgas (p)ppGpp, tasemete kvantifitseerimiseks. Rakendades seda metoodikat uuriti nukleotiidide taset bakterite kasvul ning antibiootikumitöötluse käigus. Nukleotiidide, sealhulgas (p)ppGpp taseme kvantifitseerimiseks töötati välja HPLC-l põhinev meetod. Nukleotiidide kvantifitseerimise meetodid sisaldavad kolme etappi: proovi kogumine, nukleotiidide ekstraheerimine ja kvantifitseerimine. Kogumisetapis filtreeriti bakterikultuur ja nukleotiidide ekstraheerimiseks viidi filter äädikhappesse. (p)ppGpp kvantifitseerimiseks rakendati HPLC metoodikat 5 µm 4,6 x 150 mm tugeval anioonvahetuskolonnil. Teiste nukleotiidide tuvastamiseks ja kvantifitseerimiseks kasutati ioon-paar pöördfaasi (IPRP) kromatograafiat Kinetex C18 2,6 µm 4,6 x 150 mm kolonnil. Kasutades väljatöötatud metoodikaid uuriti nukleotiidide tasemete muutust bakterite stressivastuse korral. Soolekepikesel (Escherichia coli) analüüsiti nukleotiidide tasemeid kasvukõvera erinevates faasides ja aminohapete nälja puhul. Aminohapete nälja puhul täheldati kiiret (p)ppGpp taseme tõusu. Translatsiooni inhibeerivate antibiootikumide (tiostreptooni, klooramfenikooli ja tetratsükliini) mõju (p)ppGpp ja teiste nukleotiidide tasemetele bakterirakus uuriti nii Gram-negatiivsetes kui ka Gram-positiivsetes bakterites, esindajateks vastavalt E.coli ja Bacillus subtilis. (p)ppGpp kuhjumise indutseerimiseks kasutati eeltöötlust muprirotsiiniga. Seejärel lisati uuritav antibiootikum subinhibeerivas kontsentratsioonis. Mõlema bakteriliigi korral pidurdasid kõik testitud translatsiooni inhibiitorid (p)ppGpp kuhjumist. Meie uurimisrühma bioinformaatiline analüüs tuvastas, et mõnedes bakteriliikides on RSH ensüümid, millel on ainult (p)ppGpp sünteesi eest vastutav osa. Leiti, et selline ensüüm bakteris Cellulomonas marina võib fosforüleerida ka adenosiini, tekitades molekuli ppApp. Koos paralleelselt ilmunud töödega teistest laboritest on alust arvata, et tegemist on uudse regulaatornukleotiidiga. Selle nukleotiidi täpse rolli… Advisors/Committee Members: Tenson, Tanel, juhendaja (advisor), Hauryliuk, Vasili, juhendaja (advisor).

Subjects/Keywords: bacteria; nucleotides; high pressure liquid chromatography

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APA (6^th Edition):

Alves Oliveira, S. R. (2020). HPLC analysis of bacterial alarmone nucleotide (p)ppGpp and its toxic analogue ppApp . (Thesis). Tartu University. Retrieved from http://hdl.handle.net/10062/69497

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Alves Oliveira, Sofia Raquel. “HPLC analysis of bacterial alarmone nucleotide (p)ppGpp and its toxic analogue ppApp .” 2020. Thesis, Tartu University. Accessed March 06, 2021. http://hdl.handle.net/10062/69497.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Alves Oliveira, Sofia Raquel. “HPLC analysis of bacterial alarmone nucleotide (p)ppGpp and its toxic analogue ppApp .” 2020. Web. 06 Mar 2021.

Vancouver:

Alves Oliveira SR. HPLC analysis of bacterial alarmone nucleotide (p)ppGpp and its toxic analogue ppApp . [Internet] [Thesis]. Tartu University; 2020. [cited 2021 Mar 06]. Available from: http://hdl.handle.net/10062/69497.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Alves Oliveira SR. HPLC analysis of bacterial alarmone nucleotide (p)ppGpp and its toxic analogue ppApp . [Thesis]. Tartu University; 2020. Available from: http://hdl.handle.net/10062/69497

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Open Access Theses and Dissertations