subject:(Nucleic acids)

University of Oxford

1. Tyburn, Agnes. Synthesis and properties of nucleic acid analogues containing artificial backbones.

Degree: PhD, 2019, University of Oxford

URL: http://ora.ox.ac.uk/objects/uuid:4f940f31-c5ea-4211-b7a7-58897aeb4191 ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.770812

► Nucleic acid analogues are very attractive molecules for therapeutic applications as in principle they can specifically inhibit the expression of virtually any gene through antisense… (more)

Subjects/Keywords: Nucleic acids

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Tyburn, A. (2019). Synthesis and properties of nucleic acid analogues containing artificial backbones. (Doctoral Dissertation). University of Oxford. Retrieved from http://ora.ox.ac.uk/objects/uuid:4f940f31-c5ea-4211-b7a7-58897aeb4191 ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.770812

Chicago Manual of Style (16^th Edition):

Tyburn, Agnes. “Synthesis and properties of nucleic acid analogues containing artificial backbones.” 2019. Doctoral Dissertation, University of Oxford. Accessed January 15, 2021. http://ora.ox.ac.uk/objects/uuid:4f940f31-c5ea-4211-b7a7-58897aeb4191 ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.770812.

MLA Handbook (7^th Edition):

Tyburn, Agnes. “Synthesis and properties of nucleic acid analogues containing artificial backbones.” 2019. Web. 15 Jan 2021.

Vancouver:

Tyburn A. Synthesis and properties of nucleic acid analogues containing artificial backbones. [Internet] [Doctoral dissertation]. University of Oxford; 2019. [cited 2021 Jan 15]. Available from: http://ora.ox.ac.uk/objects/uuid:4f940f31-c5ea-4211-b7a7-58897aeb4191 ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.770812.

Council of Science Editors:

Tyburn A. Synthesis and properties of nucleic acid analogues containing artificial backbones. [Doctoral Dissertation]. University of Oxford; 2019. Available from: http://ora.ox.ac.uk/objects/uuid:4f940f31-c5ea-4211-b7a7-58897aeb4191 ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.770812

Rutgers University

2. Panteva, Maria T., 1988-. Development and application of computational methods to study nucleic acid structure and function.

Degree: PhD, Chemistry and Chemical Biology, 2016, Rutgers University

URL: https://rucore.libraries.rutgers.edu/rutgers-lib/49273/

►

Molecular dynamics simulation is a powerful computational tool for studying biomolecular structure and function. Nucleic acid systems present special challenges since they are characterized by… (more)

▼

Molecular dynamics simulation is a powerful computational tool for studying biomolecular structure and function. Nucleic acid systems present special challenges since they are characterized by strong electrostatic forces and their structure and dynamics is sensitive to the surrounding ion environment. This dissertation is composed of a set of projects that, taken together, advance the state-of-the art modeling of nucleic acid systems. A common theme of this research is to provide a detailed atomistic description of RNA catalysis by applying a multi-scale modeling strategy. Computational RNA enzymology is complex in nature and requires consideration of many factors including direct and indirect interaction with ions, changes in residue protonation states, and conformational changes. This work aims to answer questions related to all three of these factors, which together with the chemical steps of the reaction, define the “problem space” of RNA enzymology. Chapters 3 and 4 represent a two step strategy for developing more balanced divalent ion parameters for nucleic acid simulations. In Chapter 3, we first evaluate the ability of 17 different Mg2+ non-bonded models to simultaneously reproduce structural, thermodynamic, kinetic and mass transport properties of the ion in solution. Chapter 4 introduces a set of newly developed Mg2+, Mn2+, Zn2+ and Cd2+ parameters that are accurate and balanced for interaction with nucleic acids, which are based on the Mg2+ model that performed best in aqueous solution in Chapter 3. In Chapter 5, molecular dynamics simulations are utilized to probe ion competition around nucleic acids and validate the ion models against experimental “ion counting” experiments. The effect of solvent model, salt conditions, and tether length on the structure and dynamics of a helix-junction-helix system is explored in Chapter 6. Chapter 7 concludes the thesis with a computational mutagenesis study of the glmS self-cleaving riboswitch conducted at several stages along the reaction path.

Advisors/Committee Members: York, Darrin M. (chair).

Subjects/Keywords: Nucleic acids

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Panteva, Maria T., 1. (2016). Development and application of computational methods to study nucleic acid structure and function. (Doctoral Dissertation). Rutgers University. Retrieved from https://rucore.libraries.rutgers.edu/rutgers-lib/49273/

Chicago Manual of Style (16^th Edition):

Panteva, Maria T., 1988-. “Development and application of computational methods to study nucleic acid structure and function.” 2016. Doctoral Dissertation, Rutgers University. Accessed January 15, 2021. https://rucore.libraries.rutgers.edu/rutgers-lib/49273/.

MLA Handbook (7^th Edition):

Panteva, Maria T., 1988-. “Development and application of computational methods to study nucleic acid structure and function.” 2016. Web. 15 Jan 2021.

Vancouver:

Panteva, Maria T. 1. Development and application of computational methods to study nucleic acid structure and function. [Internet] [Doctoral dissertation]. Rutgers University; 2016. [cited 2021 Jan 15]. Available from: https://rucore.libraries.rutgers.edu/rutgers-lib/49273/.

Council of Science Editors:

Panteva, Maria T. 1. Development and application of computational methods to study nucleic acid structure and function. [Doctoral Dissertation]. Rutgers University; 2016. Available from: https://rucore.libraries.rutgers.edu/rutgers-lib/49273/

Massey University

3. Doluca, Osman. Twisted intercalating nucleic acids (TINA) in guanosine-rich oligonucleotides : a thesis submitted in the partial fulfilment of the requirements for the degree of Doctor of Philosophy in Chemistry, Massey University, Palmerston North, New Zealand.

Degree: 2013, Massey University

URL: http://hdl.handle.net/10179/4725

► The main role in the structural diversity of DNA molecules belongs to guanosines due to their array of hydrogen bond donors and acceptors, large aromatic… (more)

▼ The main role in the structural diversity of DNA molecules belongs to guanosines due to their array of hydrogen bond donors and acceptors, large aromatic surface and ability to adopt syn or anti conformations. These properties lead to the formation of various DNA topologies such as triplexes or G-quadruplexes by guanosine-rich oligonucleotides. For a long time these secondary structures were mainly considered to be a fascinating phenomenon with little practical use; it was subsequently realised that these structures are likely to be formed under physiological conditions and therefore might be involved in many important biological processes, including genome recombination, telomere stability and regulation of gene expression. Thus, there is a growing interest in development and control of these non-traditional nucleic acid structures. Although the secondary structures of nucleic acids can be controlled to a certain extent by the careful design of oligonucleotide sequence this strategy alone is not always sufficient. In this thesis we investigated how to control the assemblies of guanosine-rich oligonucleotides using a novel tool, twisted intercalating nucleic acids (TINAs). The incorporation of pyrene-containing TINA monomers into guanosine-rich oligonucleotides led to the formation of stable triplexes or G-quadruplexes depending on the position of TINA monomers. In the light of our results, we have established a set of rules that helps to create a desired structure of guanosine-rich oligonucleotides using TINA molecules. In the second half of the thesis we focused on expanding the functionality of TINA conjugated oligonucleotides. In terms of fluorescence, we synthesised several fluorescently-silent triplex-forming oligonucleotides (TFOs) equipped with a dye at different positions in the DNA. Fluorescence properties were strongly dependent on the position of the dye. These fluorescently silent TFOs showed up to an 18-fold increase in fluorescent intensity upon triplex formation. These findings lay the foundation for the future design of artificial DNA sequences for expanding the repertoire of DNA secondary structures and function.

Subjects/Keywords: Oligonucleotides; Nucleic acids

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Doluca, O. (2013). Twisted intercalating nucleic acids (TINA) in guanosine-rich oligonucleotides : a thesis submitted in the partial fulfilment of the requirements for the degree of Doctor of Philosophy in Chemistry, Massey University, Palmerston North, New Zealand. (Thesis). Massey University. Retrieved from http://hdl.handle.net/10179/4725

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Doluca, Osman. “Twisted intercalating nucleic acids (TINA) in guanosine-rich oligonucleotides : a thesis submitted in the partial fulfilment of the requirements for the degree of Doctor of Philosophy in Chemistry, Massey University, Palmerston North, New Zealand. ” 2013. Thesis, Massey University. Accessed January 15, 2021. http://hdl.handle.net/10179/4725.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Doluca, Osman. “Twisted intercalating nucleic acids (TINA) in guanosine-rich oligonucleotides : a thesis submitted in the partial fulfilment of the requirements for the degree of Doctor of Philosophy in Chemistry, Massey University, Palmerston North, New Zealand. ” 2013. Web. 15 Jan 2021.

Vancouver:

Doluca O. Twisted intercalating nucleic acids (TINA) in guanosine-rich oligonucleotides : a thesis submitted in the partial fulfilment of the requirements for the degree of Doctor of Philosophy in Chemistry, Massey University, Palmerston North, New Zealand. [Internet] [Thesis]. Massey University; 2013. [cited 2021 Jan 15]. Available from: http://hdl.handle.net/10179/4725.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Doluca O. Twisted intercalating nucleic acids (TINA) in guanosine-rich oligonucleotides : a thesis submitted in the partial fulfilment of the requirements for the degree of Doctor of Philosophy in Chemistry, Massey University, Palmerston North, New Zealand. [Thesis]. Massey University; 2013. Available from: http://hdl.handle.net/10179/4725

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Oregon State University

4. Von Borstel, R. C. Effect of nucleic acid and nuclease on the cellular proliferation in the chick embryo otic vesicle.

Degree: MS, Zoology, 1949, Oregon State University

URL: http://hdl.handle.net/1957/53146

Subjects/Keywords: Nucleic acids

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Von Borstel, R. C. (1949). Effect of nucleic acid and nuclease on the cellular proliferation in the chick embryo otic vesicle. (Masters Thesis). Oregon State University. Retrieved from http://hdl.handle.net/1957/53146

Chicago Manual of Style (16^th Edition):

Von Borstel, R C. “Effect of nucleic acid and nuclease on the cellular proliferation in the chick embryo otic vesicle.” 1949. Masters Thesis, Oregon State University. Accessed January 15, 2021. http://hdl.handle.net/1957/53146.

MLA Handbook (7^th Edition):

Von Borstel, R C. “Effect of nucleic acid and nuclease on the cellular proliferation in the chick embryo otic vesicle.” 1949. Web. 15 Jan 2021.

Vancouver:

Von Borstel RC. Effect of nucleic acid and nuclease on the cellular proliferation in the chick embryo otic vesicle. [Internet] [Masters thesis]. Oregon State University; 1949. [cited 2021 Jan 15]. Available from: http://hdl.handle.net/1957/53146.

Council of Science Editors:

Von Borstel RC. Effect of nucleic acid and nuclease on the cellular proliferation in the chick embryo otic vesicle. [Masters Thesis]. Oregon State University; 1949. Available from: http://hdl.handle.net/1957/53146

Oregon State University

5. Vincent, Walter Sampson. The localization of ribo-nucleic acid in the ovary of the prepubertal rat.

Degree: MS, Zoology, 1948, Oregon State University

URL: http://hdl.handle.net/1957/53376

Subjects/Keywords: Nucleic acids

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Vincent, W. S. (1948). The localization of ribo-nucleic acid in the ovary of the prepubertal rat. (Masters Thesis). Oregon State University. Retrieved from http://hdl.handle.net/1957/53376

Chicago Manual of Style (16^th Edition):

Vincent, Walter Sampson. “The localization of ribo-nucleic acid in the ovary of the prepubertal rat.” 1948. Masters Thesis, Oregon State University. Accessed January 15, 2021. http://hdl.handle.net/1957/53376.

MLA Handbook (7^th Edition):

Vincent, Walter Sampson. “The localization of ribo-nucleic acid in the ovary of the prepubertal rat.” 1948. Web. 15 Jan 2021.

Vancouver:

Vincent WS. The localization of ribo-nucleic acid in the ovary of the prepubertal rat. [Internet] [Masters thesis]. Oregon State University; 1948. [cited 2021 Jan 15]. Available from: http://hdl.handle.net/1957/53376.

Council of Science Editors:

Vincent WS. The localization of ribo-nucleic acid in the ovary of the prepubertal rat. [Masters Thesis]. Oregon State University; 1948. Available from: http://hdl.handle.net/1957/53376

Cornell University

6. Tran, Thua. Nucleic Acid-Protein Hybrid Materials.

Degree: PhD, Agricultural and Biological Engineering, 2012, Cornell University

URL: http://hdl.handle.net/1813/31498

► DNA nanotechnology was used to interface with proteins through DNAprotein hybrid molecules for protein detection and protein intracellular delivery. EZZ protein, which can bind to… (more)

Subjects/Keywords: Nucleic Acids; Protein; Materials

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Tran, T. (2012). Nucleic Acid-Protein Hybrid Materials. (Doctoral Dissertation). Cornell University. Retrieved from http://hdl.handle.net/1813/31498

Chicago Manual of Style (16^th Edition):

Tran, Thua. “Nucleic Acid-Protein Hybrid Materials.” 2012. Doctoral Dissertation, Cornell University. Accessed January 15, 2021. http://hdl.handle.net/1813/31498.

MLA Handbook (7^th Edition):

Tran, Thua. “Nucleic Acid-Protein Hybrid Materials.” 2012. Web. 15 Jan 2021.

Vancouver:

Tran T. Nucleic Acid-Protein Hybrid Materials. [Internet] [Doctoral dissertation]. Cornell University; 2012. [cited 2021 Jan 15]. Available from: http://hdl.handle.net/1813/31498.

Council of Science Editors:

Tran T. Nucleic Acid-Protein Hybrid Materials. [Doctoral Dissertation]. Cornell University; 2012. Available from: http://hdl.handle.net/1813/31498

Oregon State University

7. Thompson, Cynthia Sue. Oligoribonucleotides directed at ribosomal protein binding sites as inhibitors of ribosomal small subunit assembly and as possible antibiotics.

Degree: PhD, Biochemistry and Biophysics, 2000, Oregon State University

URL: http://hdl.handle.net/1957/33019

► The effects of oligoribonucleotides of identical or similar sequences to a ribosomal protein binding site, specifically S8, on prokaryotic 30S ribosomal assembly were investigated in… (more)

▼ The effects of oligoribonucleotides of identical or similar sequences to a ribosomal protein binding site, specifically S8, on prokaryotic 30S ribosomal assembly were investigated in Escherichia coli. The oligoribonucleotides were expected to bind to the target protein S8 and block its incorporation into the 30S subunit, resulting in a nonfunctional particle. 30S assembly has been proposed to occur by similar pathways both in vivo and in vitro. If this block in assembly by the oligoribonucleotides occurs in vitro, it would also occur in vivo and could possibly lead to new modes of antibiotic therapy. The binding site of S8 was delivered into E. coli through a phagemid delivery system. The S8 binding site was cloned into a PBS+ vector under control of a lacZ promoter. The same phagemid with the ribosomal binding site of the lacZ gene deleted (the Shine-Dalgarno sequence and translational start site deleted) was also cloned and both transformed into E. coli. Growth experiments were performed in the presence and absence of the inducer isopropylthiogalactoside (IPTG). No change in growth upon induction of the phagemids was observed. Northern blot analysis was performed on cell lysates. By using a probe complementary to the S8 binding site and a probe 3' to the cloned S8 binding site, specific to the lacZ message, S8 binding site message from PBS-S8 was observed but not in amounts comparable to those of 16S rRNA. The phagemid with the deleted ribosomal binding site produced no message corresponding to either the S8 binding site or lacZ mRNA, possibly due to endonucleolytic attack upon the message. Omission of protein S8 in an in vitro reconstitution reaction results in subunits that show an 87-98% reduction in phenylalanine incorporation in the polyphenylalanine synthesis assay. This severe reduction of function was also used to test the hypothesis. Reconstitutions were performed in the presence of three unmodified oligoribonucleotides (CT4, CT10, CT11) identical or similar in sequence to the S8 binding site and assayed. None of the oligoribonucleotides showed a reduction of function when added at up to a 10:1 ratio of oligoribonucleotide to protein. CT11 was assayed to a 20:1 ratio of oligoribonucleotide to 30S proteins (TP30) and showed a 20% decrease of function at that ratio. Filter-binding assays were performed to test whether the oligoribonucleotides were binding to a protein in the TP30 mixture. The oligoribonucleotides bind protein but at much higher protein concentrations than 16S rRNA, signifying a lower affinity for the oligoribonuc1eotides. These data suggest that the oligoribonucleotides are binding to a protein but not with high enough affinity to compete with 16S rRNA. The concentration needed to be affective as an antibiotic would be too great to be practical. Advisors/Committee Members: Mathews, Christopher K. (advisor), Giovannoni, Steve (committee member).

Subjects/Keywords: Nucleic acids

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Thompson, C. S. (2000). Oligoribonucleotides directed at ribosomal protein binding sites as inhibitors of ribosomal small subunit assembly and as possible antibiotics. (Doctoral Dissertation). Oregon State University. Retrieved from http://hdl.handle.net/1957/33019

Chicago Manual of Style (16^th Edition):

Thompson, Cynthia Sue. “Oligoribonucleotides directed at ribosomal protein binding sites as inhibitors of ribosomal small subunit assembly and as possible antibiotics.” 2000. Doctoral Dissertation, Oregon State University. Accessed January 15, 2021. http://hdl.handle.net/1957/33019.

MLA Handbook (7^th Edition):

Thompson, Cynthia Sue. “Oligoribonucleotides directed at ribosomal protein binding sites as inhibitors of ribosomal small subunit assembly and as possible antibiotics.” 2000. Web. 15 Jan 2021.

Vancouver:

Thompson CS. Oligoribonucleotides directed at ribosomal protein binding sites as inhibitors of ribosomal small subunit assembly and as possible antibiotics. [Internet] [Doctoral dissertation]. Oregon State University; 2000. [cited 2021 Jan 15]. Available from: http://hdl.handle.net/1957/33019.

Council of Science Editors:

Thompson CS. Oligoribonucleotides directed at ribosomal protein binding sites as inhibitors of ribosomal small subunit assembly and as possible antibiotics. [Doctoral Dissertation]. Oregon State University; 2000. Available from: http://hdl.handle.net/1957/33019

Boston College

8. Pal, Ayan. Synthesis Of Nucleoside Analogues: Glycosylation, Rigid Nucleosides And Janus Wedge Derivatives.

Degree: PhD, Chemistry, 2012, Boston College

URL: http://dlib.bc.edu/islandora/object/bc-ir:101522

► Nucleic Acids are unique biopolymers capable of encoding and transferring genetic information from one generation to the next for every form of life. This fascinating… (more)

Subjects/Keywords: Nucleic Acids; Nucleotides; Nucleotsides

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Pal, A. (2012). Synthesis Of Nucleoside Analogues: Glycosylation, Rigid Nucleosides And Janus Wedge Derivatives. (Doctoral Dissertation). Boston College. Retrieved from http://dlib.bc.edu/islandora/object/bc-ir:101522

Chicago Manual of Style (16^th Edition):

Pal, Ayan. “Synthesis Of Nucleoside Analogues: Glycosylation, Rigid Nucleosides And Janus Wedge Derivatives.” 2012. Doctoral Dissertation, Boston College. Accessed January 15, 2021. http://dlib.bc.edu/islandora/object/bc-ir:101522.

MLA Handbook (7^th Edition):

Pal, Ayan. “Synthesis Of Nucleoside Analogues: Glycosylation, Rigid Nucleosides And Janus Wedge Derivatives.” 2012. Web. 15 Jan 2021.

Vancouver:

Pal A. Synthesis Of Nucleoside Analogues: Glycosylation, Rigid Nucleosides And Janus Wedge Derivatives. [Internet] [Doctoral dissertation]. Boston College; 2012. [cited 2021 Jan 15]. Available from: http://dlib.bc.edu/islandora/object/bc-ir:101522.

Council of Science Editors:

Pal A. Synthesis Of Nucleoside Analogues: Glycosylation, Rigid Nucleosides And Janus Wedge Derivatives. [Doctoral Dissertation]. Boston College; 2012. Available from: http://dlib.bc.edu/islandora/object/bc-ir:101522

Boston College

9. Yueh, Han. Synthesis and Study of Rigidified Nucleosides Analogues for Probing the Importance of the Deoxyribose DNA Backbone.

Degree: PhD, Chemistry, 2012, Boston College

URL: http://dlib.bc.edu/islandora/object/bc-ir:101475

► Nucleic acids are the only biopolymers capable of encoding and transferring information, this property has placed them at the fundamental core of all living organisms,… (more)

Subjects/Keywords: Cyclo-Nucleosides; DNA; Nucleic Acids

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Yueh, H. (2012). Synthesis and Study of Rigidified Nucleosides Analogues for Probing the Importance of the Deoxyribose DNA Backbone. (Doctoral Dissertation). Boston College. Retrieved from http://dlib.bc.edu/islandora/object/bc-ir:101475

Chicago Manual of Style (16^th Edition):

Yueh, Han. “Synthesis and Study of Rigidified Nucleosides Analogues for Probing the Importance of the Deoxyribose DNA Backbone.” 2012. Doctoral Dissertation, Boston College. Accessed January 15, 2021. http://dlib.bc.edu/islandora/object/bc-ir:101475.

MLA Handbook (7^th Edition):

Yueh, Han. “Synthesis and Study of Rigidified Nucleosides Analogues for Probing the Importance of the Deoxyribose DNA Backbone.” 2012. Web. 15 Jan 2021.

Vancouver:

Yueh H. Synthesis and Study of Rigidified Nucleosides Analogues for Probing the Importance of the Deoxyribose DNA Backbone. [Internet] [Doctoral dissertation]. Boston College; 2012. [cited 2021 Jan 15]. Available from: http://dlib.bc.edu/islandora/object/bc-ir:101475.

Council of Science Editors:

Yueh H. Synthesis and Study of Rigidified Nucleosides Analogues for Probing the Importance of the Deoxyribose DNA Backbone. [Doctoral Dissertation]. Boston College; 2012. Available from: http://dlib.bc.edu/islandora/object/bc-ir:101475

Boston College

10. Li, Yiran. Synthesis of Cyclo and Backbone Extended Nucleosides.

Degree: MS, Chemistry, 2013, Boston College

URL: http://dlib.bc.edu/islandora/object/bc-ir:101176

► Nucleic acids are essential biological molecules that encode and transfer genetic information from generation to generation. Intensive efforts have been made by scientists to study… (more)

Subjects/Keywords: Nucleic acids; Duplex stability

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Li, Y. (2013). Synthesis of Cyclo and Backbone Extended Nucleosides. (Masters Thesis). Boston College. Retrieved from http://dlib.bc.edu/islandora/object/bc-ir:101176

Chicago Manual of Style (16^th Edition):

Li, Yiran. “Synthesis of Cyclo and Backbone Extended Nucleosides.” 2013. Masters Thesis, Boston College. Accessed January 15, 2021. http://dlib.bc.edu/islandora/object/bc-ir:101176.

MLA Handbook (7^th Edition):

Li, Yiran. “Synthesis of Cyclo and Backbone Extended Nucleosides.” 2013. Web. 15 Jan 2021.

Vancouver:

Li Y. Synthesis of Cyclo and Backbone Extended Nucleosides. [Internet] [Masters thesis]. Boston College; 2013. [cited 2021 Jan 15]. Available from: http://dlib.bc.edu/islandora/object/bc-ir:101176.

Council of Science Editors:

Li Y. Synthesis of Cyclo and Backbone Extended Nucleosides. [Masters Thesis]. Boston College; 2013. Available from: http://dlib.bc.edu/islandora/object/bc-ir:101176

11. Klyashtornyy, Vladislav. Principles of protein nucleic acid : recognition on the examples of the ribosomal protein L1 and the cold shock domain of YB-1 protein : Principes de reconnaissance de proteine acide nucléique : exemples des protéines L1 du ribosome du domaine de choc thermique de la protéine YB-1.

Degree: Docteur es, Biologie cellulaire et moléculaire, 2012, Evry-Val d'Essonne

URL: http://www.theses.fr/2011EVRY0039

►

Cette thèse est une étude structurale sur l’interaction entre 2 protéines modèles et des acides nucléiques : la protéine L1 (navette ribosome/ARN messagers) et le sous… (more)

▼

Cette thèse est une étude structurale sur l’interaction entre 2 protéines modèles et des acides nucléiques : la protéine L1 (navette ribosome/ARN messagers) et le sous domaine CSD de YB-1, protéine régulatrice de la transcription et traduction. Deux méthodes sont utilisées : i) diffraction des RX par des cristaux de complexes L1:ARN ribosomal ou messager et ii) modélisation et dynamique moléculaire pour l’interaction CSD avec des homo-ribo- ou homo-deoxyribo-nucléotides simple brin. Les méthodes sont décrites avec leurs forces et limites. Les résultats sur L1-rARN/ARN éclairent les mécanismes de régulation de la traduction en montrant des différences d’affinité pour l’ARN des sous domaines I et II de L1. L’analyse de mutants de L1 dans le site de liaison à l’ARN du sous domaine I éclairent la nature des liaisons non covalentes sous tendant l’affinité de ce sous domaine pour l’ARN et souligne l’importance de la structure de L1, de sa « complémentarité » avec l’ARN et de liaisons hydrogènes non accessibles au solvant. Les travaux de modélisation et de dynamique moléculaire sur l’interaction CSD:acides nucléiques montrent que la séquence nucléotidique module l’affinité de ce complexe, l’oligonucléotide de type oligoG donnant le complexe le plus stable suivi des séquences de type oligoU et oligoA puis des oligoT et oligoC. L’orientation du brin d’ARN par rapport au CSD impacte aussi la stabilité du complexe. Une analyse des surfaces d’interaction et de la nature des liaisons intermoléculaires, montre que des principes similaires guident l’interaction L1:ARN et CSD:acides nucléiques : géométrie complémentaire des partenaires et liaisons hydrogène protégées du solvant.

This thesis is a structural study on the interaction between two model proteins and nucleic acids: the L1 protein (shuttle ribosome/mRNA) and the CSD subdomain of YB-1, a protein that regulates transcription and translation. Two methods are used: i) X-ray diffraction by crystals of L1:ribosomal or messenger RNA complexes and ii) molecular modeling and dynamics for the CSD interaction with homo-ribo or homo-deoxyribo- single-stranded nucleotide. The methods are described with their strengths and limitations. Results on L1-rARN/ARN enlighten the mechanisms regulating translation by showing differences in affinity for RNA of the subdomains I and II of L1. Analyses of L1 mutants in the RNA binding site from the subdomain I illuminate the nature of non-covalent bonds subtending the affinity of this subdomain for RNA and stress the importance of the structure of L1, its "complementarity" with RNA and of hydrogen bonds not accessible to the solvent. Molecular modeling and dynamics of the CSD:nucleic acids interaction shows that the nucleotide sequence modulates the affinity of the complex, oligoG giving the most stable complex followed by oligoU and then oligoA or oligoT and oligoC. The orientation of the RNA strand relative to the CSD also impacts the stability of the complex. An analysis of the interaction surfaces and of the nature of intermolecular bonds,…

Advisors/Committee Members: Manivet, Philippe (thesis director), Nikonov, Stanislav (thesis director).

Subjects/Keywords: Modélisation; Modelization; Nucleic acids; Proteins

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Klyashtornyy, V. (2012). Principles of protein nucleic acid : recognition on the examples of the ribosomal protein L1 and the cold shock domain of YB-1 protein : Principes de reconnaissance de proteine acide nucléique : exemples des protéines L1 du ribosome du domaine de choc thermique de la protéine YB-1. (Doctoral Dissertation). Evry-Val d'Essonne. Retrieved from http://www.theses.fr/2011EVRY0039

Chicago Manual of Style (16^th Edition):

Klyashtornyy, Vladislav. “Principles of protein nucleic acid : recognition on the examples of the ribosomal protein L1 and the cold shock domain of YB-1 protein : Principes de reconnaissance de proteine acide nucléique : exemples des protéines L1 du ribosome du domaine de choc thermique de la protéine YB-1.” 2012. Doctoral Dissertation, Evry-Val d'Essonne. Accessed January 15, 2021. http://www.theses.fr/2011EVRY0039.

MLA Handbook (7^th Edition):

Klyashtornyy, Vladislav. “Principles of protein nucleic acid : recognition on the examples of the ribosomal protein L1 and the cold shock domain of YB-1 protein : Principes de reconnaissance de proteine acide nucléique : exemples des protéines L1 du ribosome du domaine de choc thermique de la protéine YB-1.” 2012. Web. 15 Jan 2021.

Vancouver:

Klyashtornyy V. Principles of protein nucleic acid : recognition on the examples of the ribosomal protein L1 and the cold shock domain of YB-1 protein : Principes de reconnaissance de proteine acide nucléique : exemples des protéines L1 du ribosome du domaine de choc thermique de la protéine YB-1. [Internet] [Doctoral dissertation]. Evry-Val d'Essonne; 2012. [cited 2021 Jan 15]. Available from: http://www.theses.fr/2011EVRY0039.

Council of Science Editors:

Klyashtornyy V. Principles of protein nucleic acid : recognition on the examples of the ribosomal protein L1 and the cold shock domain of YB-1 protein : Principes de reconnaissance de proteine acide nucléique : exemples des protéines L1 du ribosome du domaine de choc thermique de la protéine YB-1. [Doctoral Dissertation]. Evry-Val d'Essonne; 2012. Available from: http://www.theses.fr/2011EVRY0039

University of Oxford

12. Dysko, Anna Monika. Synthesis and properties of oligonucleotides containing triazole backbone linkages and 2'-modifications for therapeutic applications.

Degree: PhD, 2016, University of Oxford

URL: https://ora.ox.ac.uk/objects/uuid:20fc1203-9751-4654-b497-5f4d97f874a1 ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.730453

► Antisense oligonucleotides are short strands of DNA, which bind to their complementary mRNA target to prevent protein translation. Although conceptually appealing, for their practical use… (more)

Subjects/Keywords: 572.8; Antisense nucleic acids

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Dysko, A. M. (2016). Synthesis and properties of oligonucleotides containing triazole backbone linkages and 2'-modifications for therapeutic applications. (Doctoral Dissertation). University of Oxford. Retrieved from https://ora.ox.ac.uk/objects/uuid:20fc1203-9751-4654-b497-5f4d97f874a1 ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.730453

Chicago Manual of Style (16^th Edition):

Dysko, Anna Monika. “Synthesis and properties of oligonucleotides containing triazole backbone linkages and 2'-modifications for therapeutic applications.” 2016. Doctoral Dissertation, University of Oxford. Accessed January 15, 2021. https://ora.ox.ac.uk/objects/uuid:20fc1203-9751-4654-b497-5f4d97f874a1 ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.730453.

MLA Handbook (7^th Edition):

Dysko, Anna Monika. “Synthesis and properties of oligonucleotides containing triazole backbone linkages and 2'-modifications for therapeutic applications.” 2016. Web. 15 Jan 2021.

Vancouver:

Dysko AM. Synthesis and properties of oligonucleotides containing triazole backbone linkages and 2'-modifications for therapeutic applications. [Internet] [Doctoral dissertation]. University of Oxford; 2016. [cited 2021 Jan 15]. Available from: https://ora.ox.ac.uk/objects/uuid:20fc1203-9751-4654-b497-5f4d97f874a1 ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.730453.

Council of Science Editors:

Dysko AM. Synthesis and properties of oligonucleotides containing triazole backbone linkages and 2'-modifications for therapeutic applications. [Doctoral Dissertation]. University of Oxford; 2016. Available from: https://ora.ox.ac.uk/objects/uuid:20fc1203-9751-4654-b497-5f4d97f874a1 ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.730453

Rice University

13. Wu, Ruojia. Simple, Multiplexed, and Ultraspecific Nucleic Acid-based Diagnostic Technologies.

Degree: PhD, Engineering, 2017, Rice University

URL: http://hdl.handle.net/1911/105491

► The economical and high-throughput detection and quantitation of disease-relevant nucleic acid sequences is a key goal in the road to widespread adoption of precision medicine,… (more)

Subjects/Keywords: Molecular diagnostics; nucleic acids

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Wu, R. (2017). Simple, Multiplexed, and Ultraspecific Nucleic Acid-based Diagnostic Technologies. (Doctoral Dissertation). Rice University. Retrieved from http://hdl.handle.net/1911/105491

Chicago Manual of Style (16^th Edition):

Wu, Ruojia. “Simple, Multiplexed, and Ultraspecific Nucleic Acid-based Diagnostic Technologies.” 2017. Doctoral Dissertation, Rice University. Accessed January 15, 2021. http://hdl.handle.net/1911/105491.

MLA Handbook (7^th Edition):

Wu, Ruojia. “Simple, Multiplexed, and Ultraspecific Nucleic Acid-based Diagnostic Technologies.” 2017. Web. 15 Jan 2021.

Vancouver:

Wu R. Simple, Multiplexed, and Ultraspecific Nucleic Acid-based Diagnostic Technologies. [Internet] [Doctoral dissertation]. Rice University; 2017. [cited 2021 Jan 15]. Available from: http://hdl.handle.net/1911/105491.

Council of Science Editors:

Wu R. Simple, Multiplexed, and Ultraspecific Nucleic Acid-based Diagnostic Technologies. [Doctoral Dissertation]. Rice University; 2017. Available from: http://hdl.handle.net/1911/105491

Michigan State University

14. Becker, Veryl Edwin. Studies on nucleic acids of plants.

Degree: PhD, Department of Botany and Plant Pathology, 1967, Michigan State University

URL: http://etd.lib.msu.edu/islandora/object/etd:34911

Subjects/Keywords: Nucleic acids

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Becker, V. E. (1967). Studies on nucleic acids of plants. (Doctoral Dissertation). Michigan State University. Retrieved from http://etd.lib.msu.edu/islandora/object/etd:34911

Chicago Manual of Style (16^th Edition):

Becker, Veryl Edwin. “Studies on nucleic acids of plants.” 1967. Doctoral Dissertation, Michigan State University. Accessed January 15, 2021. http://etd.lib.msu.edu/islandora/object/etd:34911.

MLA Handbook (7^th Edition):

Becker, Veryl Edwin. “Studies on nucleic acids of plants.” 1967. Web. 15 Jan 2021.

Vancouver:

Becker VE. Studies on nucleic acids of plants. [Internet] [Doctoral dissertation]. Michigan State University; 1967. [cited 2021 Jan 15]. Available from: http://etd.lib.msu.edu/islandora/object/etd:34911.

Council of Science Editors:

Becker VE. Studies on nucleic acids of plants. [Doctoral Dissertation]. Michigan State University; 1967. Available from: http://etd.lib.msu.edu/islandora/object/etd:34911

Michigan State University

15. Halloin, John McDonell. The 5'-linked termini of plant ribosmal ribonucleic acids.

Degree: PhD, Department of Botany and Plant Pathology, 1968, Michigan State University

URL: http://etd.lib.msu.edu/islandora/object/etd:40737

Subjects/Keywords: Nucleic acids

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Halloin, J. M. (1968). The 5'-linked termini of plant ribosmal ribonucleic acids. (Doctoral Dissertation). Michigan State University. Retrieved from http://etd.lib.msu.edu/islandora/object/etd:40737

Chicago Manual of Style (16^th Edition):

Halloin, John McDonell. “The 5'-linked termini of plant ribosmal ribonucleic acids.” 1968. Doctoral Dissertation, Michigan State University. Accessed January 15, 2021. http://etd.lib.msu.edu/islandora/object/etd:40737.

MLA Handbook (7^th Edition):

Halloin, John McDonell. “The 5'-linked termini of plant ribosmal ribonucleic acids.” 1968. Web. 15 Jan 2021.

Vancouver:

Halloin JM. The 5'-linked termini of plant ribosmal ribonucleic acids. [Internet] [Doctoral dissertation]. Michigan State University; 1968. [cited 2021 Jan 15]. Available from: http://etd.lib.msu.edu/islandora/object/etd:40737.

Council of Science Editors:

Halloin JM. The 5'-linked termini of plant ribosmal ribonucleic acids. [Doctoral Dissertation]. Michigan State University; 1968. Available from: http://etd.lib.msu.edu/islandora/object/etd:40737

University of British Columbia

16. Warrington, Robert Charles. Use of benzoylated deae-cellulose for the isolation of glycine transfer ribonucleic acids of yeast and for the development of new methods for sequence determination of nucleic acids.

Degree: PhD, Biochemistry and Molecular Biology, 1970, University of British Columbia

URL: http://hdl.handle.net/2429/34174

► Part I of this thesis describes a method for the isolation of a specific family of aminoacyl-tRNAs. Reaction of the aminoacyl-tRNAs with the N-hydroxysuccinimide ester… (more)

▼ Part I of this thesis describes a method for the isolation of a specific family of aminoacyl-tRNAs. Reaction of the aminoacyl-tRNAs with the N-hydroxysuccinimide ester of 2-naphthoxyacetic acid produces N-2-naphthoxyacetylaminoacy1-tRNAs which possess sufficient affinity for BD-cellulose to allow their separation from unmodified tRNA by simple chromatographic steps. The N-2-naphthoxyacetyl amino acid is removed by mild alkaline hydrolysis and the iso-acceptor tRNAs are separated by chromatography on BD-cellulose. tRNA₁Gly and tRNA₂Gly have been purified from brewer's yeast (Saccharomyces cerevisiae) by this method and a simplified procedure for the large scale isolation of these tRNAs was developed. The object of Part II of this thesis was to develop a new method of nucleotide sequence analysis based on the following steps: (i) specific introduction of hydrophobic groups onto the 3'- or 5'-terminals of polynucleotides, (ii) subsequent cleavage of the appropriately derivatized polymer under conditions which favor rupture of only one bond per molecule of polymer, (iii) separation of fragments bearing the derivative from those not bearing the derivative, (iv) resolution of derivatized fragments according to chain length, (v) complete degradation of the purified fragments from step (iv) with the reagent used to generate these fragments followed by identification of the components so liberated from each fragment, and finally, (vi) assembly of the data obtained from step (v) to order the composite fragments from 5' and 3' ends to derive an unambiguous primary sequence. It was reasoned that N-2-naphthoxyacetylglycyl-tRNA₁Gly and tRNA₂Gly could be used as a model polymer bearing an appropriate 3’-derivative, that BD-cel1u1ose chromatography could effect step (iii), that DEAE-cel1ulose-7 M urea column chromatography could effect step (iv) and that either DEAE-cellulose chromatography or combined chromatographic-electrophoretic techniques could effect step (v). A method for placing a hydrophobic derivative onto the 5’-terminus of polynucleotides needed to be developed. Further, it was necessary to discover if available endonuc1eases could degrade the model polymer in a manner approaching random, single-hit kinetics. To ascertain the feasibility of the proposed method, two studies were undertaken. First, the 3'-terminal fragments released by exhaustive digestion of N-2-naphthoxyacetylglycyl-tRNA₁Gly and tRNA₂Gly with RNase T₁ were isolated by the procedure to verify the efficiency of a number of the required steps. The fragments (from the results of the nucleostide-analysis and on the assumption that the 3'-sequence cytidy1y1(3’-5’)cytidy1y1 (3’5’adenosine is common to all functional tRNAs) are (Cp, Ap)CpCpA and (Up, Ap, Cp, Cp) CpCpA. The relative yields of the two fragments suggested they were derived from tRNA₁Gly and tRNA₂Gly, respectively. Second, a study was undertaken of the degradation products liberated from crude and purified glycyl-tRNAs as well as from crude and purified N-2-naphthoxyacety1g1ycy1-tRNAs by RNase T₁…

Subjects/Keywords: Nucleic acids

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Warrington, R. C. (1970). Use of benzoylated deae-cellulose for the isolation of glycine transfer ribonucleic acids of yeast and for the development of new methods for sequence determination of nucleic acids. (Doctoral Dissertation). University of British Columbia. Retrieved from http://hdl.handle.net/2429/34174

Chicago Manual of Style (16^th Edition):

Warrington, Robert Charles. “Use of benzoylated deae-cellulose for the isolation of glycine transfer ribonucleic acids of yeast and for the development of new methods for sequence determination of nucleic acids.” 1970. Doctoral Dissertation, University of British Columbia. Accessed January 15, 2021. http://hdl.handle.net/2429/34174.

MLA Handbook (7^th Edition):

Warrington, Robert Charles. “Use of benzoylated deae-cellulose for the isolation of glycine transfer ribonucleic acids of yeast and for the development of new methods for sequence determination of nucleic acids.” 1970. Web. 15 Jan 2021.

Vancouver:

Warrington RC. Use of benzoylated deae-cellulose for the isolation of glycine transfer ribonucleic acids of yeast and for the development of new methods for sequence determination of nucleic acids. [Internet] [Doctoral dissertation]. University of British Columbia; 1970. [cited 2021 Jan 15]. Available from: http://hdl.handle.net/2429/34174.

Council of Science Editors:

Warrington RC. Use of benzoylated deae-cellulose for the isolation of glycine transfer ribonucleic acids of yeast and for the development of new methods for sequence determination of nucleic acids. [Doctoral Dissertation]. University of British Columbia; 1970. Available from: http://hdl.handle.net/2429/34174

University of New South Wales

17. Yu, Albert. Integrative Genome-Wide Analysis of DNA Methyltransferase 3A Reveals a Novel Model of Transcriptional Regulation Modulated by Long Non-coding RNAs.

Degree: Biotechnology & Biomolecular Sciences, 2016, University of New South Wales

URL: http://handle.unsw.edu.au/1959.4/56093 ; https://unsworks.unsw.edu.au/fapi/datastream/unsworks:40011/SOURCE02?view=true

► LncRNAs are a class of nucleic acids that have been widely demonstrated to be involved in transcriptional regulation through epigenetic regulation via DNA methylation and… (more)

Subjects/Keywords: Nucleic acids; DNA methylation

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Yu, A. (2016). Integrative Genome-Wide Analysis of DNA Methyltransferase 3A Reveals a Novel Model of Transcriptional Regulation Modulated by Long Non-coding RNAs. (Masters Thesis). University of New South Wales. Retrieved from http://handle.unsw.edu.au/1959.4/56093 ; https://unsworks.unsw.edu.au/fapi/datastream/unsworks:40011/SOURCE02?view=true

Chicago Manual of Style (16^th Edition):

Yu, Albert. “Integrative Genome-Wide Analysis of DNA Methyltransferase 3A Reveals a Novel Model of Transcriptional Regulation Modulated by Long Non-coding RNAs.” 2016. Masters Thesis, University of New South Wales. Accessed January 15, 2021. http://handle.unsw.edu.au/1959.4/56093 ; https://unsworks.unsw.edu.au/fapi/datastream/unsworks:40011/SOURCE02?view=true.

MLA Handbook (7^th Edition):

Yu, Albert. “Integrative Genome-Wide Analysis of DNA Methyltransferase 3A Reveals a Novel Model of Transcriptional Regulation Modulated by Long Non-coding RNAs.” 2016. Web. 15 Jan 2021.

Vancouver:

Yu A. Integrative Genome-Wide Analysis of DNA Methyltransferase 3A Reveals a Novel Model of Transcriptional Regulation Modulated by Long Non-coding RNAs. [Internet] [Masters thesis]. University of New South Wales; 2016. [cited 2021 Jan 15]. Available from: http://handle.unsw.edu.au/1959.4/56093 ; https://unsworks.unsw.edu.au/fapi/datastream/unsworks:40011/SOURCE02?view=true.

Council of Science Editors:

Yu A. Integrative Genome-Wide Analysis of DNA Methyltransferase 3A Reveals a Novel Model of Transcriptional Regulation Modulated by Long Non-coding RNAs. [Masters Thesis]. University of New South Wales; 2016. Available from: http://handle.unsw.edu.au/1959.4/56093 ; https://unsworks.unsw.edu.au/fapi/datastream/unsworks:40011/SOURCE02?view=true

University of Texas – Austin

18. Levy, Matthew. Design and evolution of functional nucleic acids.

Degree: PhD, Cell and Molecular Biology, 2003, University of Texas – Austin

URL: http://hdl.handle.net/2152/1106

► Functional nucleic acids provide insight into the ‘RNA world,’ the proposed period in Earth’s history where RNA served both as catalyst and as the genetic… (more)

▼ Functional nucleic acids provide insight into the ‘RNA world,’ the proposed period in Earth’s history where RNA served both as catalyst and as the genetic material. Additionally, they are potentially valuable tools for biotechnical applications. Like catalytic RNA, DNA has proven capable of catalyzing chemical reactions. Using a novel ligation chemistry, we have employed in vitro selection to isolate a catalytic DNA molecule (deoxyribozyme) that is capable of forming an unnatural nucleotide linkage. In addition, we have used a combination of rational design and in vitro selection to construct effector-dependent deoxyribozymes that are allosterically activated by the small molecule ATP. These results further strengthen the idea that life arose from a nucleic acid based metabolism and also bode well for the design of more stable nucleic acid based analyte detection systems. In addition to the design of novel DNA catalysts, we have been interested in the origin of the first simple self replicating systems. Simple replicators based on short oligonucleotides as well as peptides have been demonstrated. We have designed a cross-catalytic system based on a well characterized peptide-RNA aptamer interaction, in which the peptide serves as a template for the ligation of RNA aptamer half-molecules. Our results demonstrate that the peptide could specifically enhance the rate of RNA ligation, and suggest the possibility for increased diversity of early replicators. We have also designed an autocatalytic system based on the fast and efficient RNA cleaving 10-23 deoxyribozyme. In this system, two complementary deoxyribozymes have been inactivated by circularization. The circular deoxyribozymes are capable of serving as substrates for the linear enzymes such that linearization results in a cascade of cleaving reactions that exhibits exponential growth. A selection scheme based on this reaction resulted in optimal sequence selection and demonstrates the first in vitro selection experiment conducted in the absence of proteins. Advisors/Committee Members: Ellington, Andrew D. (advisor).

Subjects/Keywords: Nucleic acids

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Levy, M. (2003). Design and evolution of functional nucleic acids. (Doctoral Dissertation). University of Texas – Austin. Retrieved from http://hdl.handle.net/2152/1106

Chicago Manual of Style (16^th Edition):

Levy, Matthew. “Design and evolution of functional nucleic acids.” 2003. Doctoral Dissertation, University of Texas – Austin. Accessed January 15, 2021. http://hdl.handle.net/2152/1106.

MLA Handbook (7^th Edition):

Levy, Matthew. “Design and evolution of functional nucleic acids.” 2003. Web. 15 Jan 2021.

Vancouver:

Levy M. Design and evolution of functional nucleic acids. [Internet] [Doctoral dissertation]. University of Texas – Austin; 2003. [cited 2021 Jan 15]. Available from: http://hdl.handle.net/2152/1106.

Council of Science Editors:

Levy M. Design and evolution of functional nucleic acids. [Doctoral Dissertation]. University of Texas – Austin; 2003. Available from: http://hdl.handle.net/2152/1106

University of British Columbia

19. Scrimgeour, Kenneth Gray. Studies on the biosynthesis of nucleic acids by Novikoff hepatoma tissue in vitro.

Degree: MS- MSc, Biochemistry and Molecular Biology, 1957, University of British Columbia

URL: http://hdl.handle.net/2429/40238

► Nucleic acid metabolism has been studied in vitro with suspensions of Novikoff rat hepatoma cells. The formation of acid soluble and nucleic acid purines from… (more)

Subjects/Keywords: Nucleic acids

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Scrimgeour, K. G. (1957). Studies on the biosynthesis of nucleic acids by Novikoff hepatoma tissue in vitro. (Masters Thesis). University of British Columbia. Retrieved from http://hdl.handle.net/2429/40238

Chicago Manual of Style (16^th Edition):

Scrimgeour, Kenneth Gray. “Studies on the biosynthesis of nucleic acids by Novikoff hepatoma tissue in vitro.” 1957. Masters Thesis, University of British Columbia. Accessed January 15, 2021. http://hdl.handle.net/2429/40238.

MLA Handbook (7^th Edition):

Scrimgeour, Kenneth Gray. “Studies on the biosynthesis of nucleic acids by Novikoff hepatoma tissue in vitro.” 1957. Web. 15 Jan 2021.

Vancouver:

Scrimgeour KG. Studies on the biosynthesis of nucleic acids by Novikoff hepatoma tissue in vitro. [Internet] [Masters thesis]. University of British Columbia; 1957. [cited 2021 Jan 15]. Available from: http://hdl.handle.net/2429/40238.

Council of Science Editors:

Scrimgeour KG. Studies on the biosynthesis of nucleic acids by Novikoff hepatoma tissue in vitro. [Masters Thesis]. University of British Columbia; 1957. Available from: http://hdl.handle.net/2429/40238

University of British Columbia

20. Paterson, Alan Robb Phillips. The metabolism of 2-C¹⁴-adenine in the adult male rat.

Degree: MA- MA, Biochemistry and Molecular Biology, 1952, University of British Columbia

URL: http://hdl.handle.net/2429/40955

► Isotopic adenine, labeled with C¹⁴ in position 2, has been prepared in three steps; (a) formylation of 4-amino - 5-imidazolecarboxamidine in aqueous C¹⁴-formic acid, (b)… (more)

Subjects/Keywords: Nucleic acids

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Paterson, A. R. P. (1952). The metabolism of 2-C¹⁴-adenine in the adult male rat. (Masters Thesis). University of British Columbia. Retrieved from http://hdl.handle.net/2429/40955

Chicago Manual of Style (16^th Edition):

Paterson, Alan Robb Phillips. “The metabolism of 2-C¹⁴-adenine in the adult male rat.” 1952. Masters Thesis, University of British Columbia. Accessed January 15, 2021. http://hdl.handle.net/2429/40955.

MLA Handbook (7^th Edition):

Paterson, Alan Robb Phillips. “The metabolism of 2-C¹⁴-adenine in the adult male rat.” 1952. Web. 15 Jan 2021.

Vancouver:

Paterson ARP. The metabolism of 2-C¹⁴-adenine in the adult male rat. [Internet] [Masters thesis]. University of British Columbia; 1952. [cited 2021 Jan 15]. Available from: http://hdl.handle.net/2429/40955.

Council of Science Editors:

Paterson ARP. The metabolism of 2-C¹⁴-adenine in the adult male rat. [Masters Thesis]. University of British Columbia; 1952. Available from: http://hdl.handle.net/2429/40955

Oregon State University

21. Hicks, Joshua M. Analysis of secondary structures in nucleic acid binding proteins and nuclear magnetic resonance investigation of helix propagation and residual motions in proteins.

Degree: PhD, Biochemistry and Biophysics, 2005, Oregon State University

URL: http://hdl.handle.net/1957/29424

Subjects/Keywords: Nucleic acids – Analysis

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Hicks, J. M. (2005). Analysis of secondary structures in nucleic acid binding proteins and nuclear magnetic resonance investigation of helix propagation and residual motions in proteins. (Doctoral Dissertation). Oregon State University. Retrieved from http://hdl.handle.net/1957/29424

Chicago Manual of Style (16^th Edition):

Hicks, Joshua M. “Analysis of secondary structures in nucleic acid binding proteins and nuclear magnetic resonance investigation of helix propagation and residual motions in proteins.” 2005. Doctoral Dissertation, Oregon State University. Accessed January 15, 2021. http://hdl.handle.net/1957/29424.

MLA Handbook (7^th Edition):

Hicks, Joshua M. “Analysis of secondary structures in nucleic acid binding proteins and nuclear magnetic resonance investigation of helix propagation and residual motions in proteins.” 2005. Web. 15 Jan 2021.

Vancouver:

Hicks JM. Analysis of secondary structures in nucleic acid binding proteins and nuclear magnetic resonance investigation of helix propagation and residual motions in proteins. [Internet] [Doctoral dissertation]. Oregon State University; 2005. [cited 2021 Jan 15]. Available from: http://hdl.handle.net/1957/29424.

Council of Science Editors:

Hicks JM. Analysis of secondary structures in nucleic acid binding proteins and nuclear magnetic resonance investigation of helix propagation and residual motions in proteins. [Doctoral Dissertation]. Oregon State University; 2005. Available from: http://hdl.handle.net/1957/29424

McMaster University

22. Chang, Dingran. Developing DNA based biosensors for Clostridium difficile detection.

Degree: PhD, 2018, McMaster University

URL: http://hdl.handle.net/11375/23769

► Over the last 20 years, the incidence of Clostridium difficile infection (CDI) has increased dramatically, making it one of the most common healthcare-associated infections. This… (more)

▼ Over the last 20 years, the incidence of Clostridium difficile infection (CDI) has increased dramatically, making it one of the most common healthcare-associated infections. This has been linked to the emergence of hypervirulent C. difficile strains. Currently, cell cytotoxicity assay (CTA) and toxigenic culture are the gold-standard methods for CDI diagnosis. However, they are time-consuming and labour-intensive. Other methods, like enzyme immunoassays (EIAs) and nucleic acid amplification-based tests (NAATs), allow for rapid testing but have poor sensitivity and/or specificity. Additionally, most of these methods target toxins or their associated genes and are unable to discriminate between epidemic and non-epidemic strains. The work described in this dissertation aims to develop easy-to-use and reliable biosensors for C. difficile, with a particular focus on epidemic strains of C. difficile. The development of the in vitro selection technique has allowed for the discovery of a big array of functional DNA, with excellent ability in both target recognition and enzymatic catalysis. My key interest is to employ functional DNA molecules as target recognition elements to develop colorimetric biosensors for C. difficile detection. The first research project aimed to develop a colorimetric detection platform that can be coupled with functional DNA molecules to achieve hypersensitive detection of different targets. This test should be easy-to-use, have broad target applicability and not require expensive equipment. To do so, a colorimetric biosensing platform was created, which takes advantage of the signal amplification ability of rolling circle amplification (RCA) and the simplicity of the classic litmus test. In the presence of the target of interest, RCA will be triggered, and the biotinylated RCA products can hybridize a number of the urease-labelled single-stranded DNA and immobilize urease onto magnetic beads through streptavidin-biotin interactions. The urease can then be used to hydrolyze urea, resulting in significant pH elevation, that can be detected easily using a litmus test. To prove the concept, we have demonstrated that this platform can be employed to visually detect thrombin and platelet-derived growth factor (PDGF) with high sensitivity, by coupling it with an anti-thrombin aptamer and an anti-PDGF aptamer, respectively. We have also shown that the biosensing platform can be incorporated into simple paper-based devices. The second project focuses on the development of a colorimetric DNA detection method for epidemic strains of C. difficile that utilizes both the polymerase chain reaction (PCR) and the litmus test. The strategy makes use of a modified set of primers for PCR to facilitate ensuing manipulations of resultant DNA amplicons: their tagging with urease and immobilization onto magnetic beads. The amplicon/urease-laden beads are then used to hydrolyze urea, resulting in an increase of pH that can be conveniently reported by a pH-sensitive dye. We have successfully applied this strategy for the… Advisors/Committee Members: Li, Yingfu, Biochemistry and Biomedical Sciences.

Subjects/Keywords: biosensor; Clostridium difficile; functional nucleic acids

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Chang, D. (2018). Developing DNA based biosensors for Clostridium difficile detection. (Doctoral Dissertation). McMaster University. Retrieved from http://hdl.handle.net/11375/23769

Chicago Manual of Style (16^th Edition):

Chang, Dingran. “Developing DNA based biosensors for Clostridium difficile detection.” 2018. Doctoral Dissertation, McMaster University. Accessed January 15, 2021. http://hdl.handle.net/11375/23769.

MLA Handbook (7^th Edition):

Chang, Dingran. “Developing DNA based biosensors for Clostridium difficile detection.” 2018. Web. 15 Jan 2021.

Vancouver:

Chang D. Developing DNA based biosensors for Clostridium difficile detection. [Internet] [Doctoral dissertation]. McMaster University; 2018. [cited 2021 Jan 15]. Available from: http://hdl.handle.net/11375/23769.

Council of Science Editors:

Chang D. Developing DNA based biosensors for Clostridium difficile detection. [Doctoral Dissertation]. McMaster University; 2018. Available from: http://hdl.handle.net/11375/23769

McMaster University

23. Tram, Kha. EXPLORING SYNTHETIC FUNCTIONAL DNA MOLECULES FOR BIOSENSOR DEVELOPMENT.

Degree: PhD, 2015, McMaster University

URL: http://hdl.handle.net/11375/16884

►

The development of the in vitro selection technique permits the creation of synthetic DNA molecules with ligand-binding capabilities (DNA aptamers), or abilities to catalyze chemical… (more)

Subjects/Keywords: DNAzyme; Aptamer; Biosensors; Functional Nucleic Acids

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Tram, K. (2015). EXPLORING SYNTHETIC FUNCTIONAL DNA MOLECULES FOR BIOSENSOR DEVELOPMENT. (Doctoral Dissertation). McMaster University. Retrieved from http://hdl.handle.net/11375/16884

Chicago Manual of Style (16^th Edition):

Tram, Kha. “EXPLORING SYNTHETIC FUNCTIONAL DNA MOLECULES FOR BIOSENSOR DEVELOPMENT.” 2015. Doctoral Dissertation, McMaster University. Accessed January 15, 2021. http://hdl.handle.net/11375/16884.

MLA Handbook (7^th Edition):

Tram, Kha. “EXPLORING SYNTHETIC FUNCTIONAL DNA MOLECULES FOR BIOSENSOR DEVELOPMENT.” 2015. Web. 15 Jan 2021.

Vancouver:

Tram K. EXPLORING SYNTHETIC FUNCTIONAL DNA MOLECULES FOR BIOSENSOR DEVELOPMENT. [Internet] [Doctoral dissertation]. McMaster University; 2015. [cited 2021 Jan 15]. Available from: http://hdl.handle.net/11375/16884.

Council of Science Editors:

Tram K. EXPLORING SYNTHETIC FUNCTIONAL DNA MOLECULES FOR BIOSENSOR DEVELOPMENT. [Doctoral Dissertation]. McMaster University; 2015. Available from: http://hdl.handle.net/11375/16884

24. Hamilton, Tatyana. Protein-nucleic acid interactions of Wilms' Tumor and TFIIIA zinc finger proteins.

Degree: Department of Biochemistry and Microbiology, 2017, University of Victoria

URL: http://hdl.handle.net/1828/8289

► This Ph.D. work represents the study of nucleic acid interactions of two zinc finger proteins: mammalian Wilms' tumor suppressor (WT1) and Xenopus transcription factor IIIA… (more)

▼ This Ph.D. work represents the study of nucleic acid interactions of two zinc finger proteins: mammalian Wilms' tumor suppressor (WT1) and Xenopus transcription factor IIIA proteins (TFIIIA). WT1 is a putative transcriptional regulatory protein which is inactivated in a subtype of Wilm s' tumours. Using selection and amplification binding assay (SAAB) we determined that the highest affinity binding sites for WT1[-KTS] consist of a 12 base pair sequence GCG-TGG-GCG-(T/G)(G/A/T)(T/G). These sequences have a four-fold higher affinity for the protein than the nonselected sequence GCG-TGG -GCG-CCC , as measured by a quantitative nitrocellulose filter binding assay. The effects of Denys-Drash syndrome (DOS) point mutations on the DNA binding activity of WT1 were determined. SAAB assay revealed that none of the DDS mutant proteins give rise to a new sequence specificity. One mutation, R394W abolishes specific binding of the protein. The remaining mutations result in reduced DNA- binding activity, ranging from 1.4 to 14-fold, which suggests that even small changes in DNA-binding activity may precipitate the clinical phenotype of Denys-Drash syndrome. Comparative analysis of the DNA binding characteristics of Wilms' tumour and Early growth response proteins was conducted . The stoichiometry of the DNA-protein complexes, their stability to dissociation, and the effects of pH, temperature and salt concentration on the equilibrium binding of these proteins to their cognate DNA sequences have been determined. Under the conditions of 0.1 M salt, p H 7.5, and 22 * C WT1-ZF has an apparent dissociation constant (Kd) of 1.14± 0.2 X 10⁻⁰⁹ M, and EGR-1 protein has a Kd of 3.55 ± 0.4 x 10⁻⁰⁹ M. In addition, we tested relative contribution of each base pair in the consensus binding site to the high affinity binding by point mutational analysis, and identified important differences that exist in the binding modes of the two proteins. Transcription factor IIIA controls the expression of the 5S ribosomal RN A genes during development of Xenopus laevis., and specifically interacts with both 5S DNA and 5S rRNA molecules. The present study assesses contributions of the central zinc fingers four through seven to specific DNA and RNA binding activities of the protein. The results demonstrate that each zinc finger in the zf 4-7 region contributes to both the high affinity DNA and RNA interactions: the largest effect on TFlllA-DNA binding (10-fold) wa s produced when zinc finger 5 of TFIIIA was replaced with the donor sequences of either p43 or WT1. However, while all the zinc fingers 4-7 contribute to the high affinity 5S rRNA binding, substitution of an α- helical portion of zinc finger 6 with the equivalent sequences from WT1 abolished RNA-binding activity of TFIIIA, suggesting that zIinc finger 6 p lay s a particularly im portant role in binding to RNA. Advisors/Committee Members: Romaniuk, Paul John (supervisor).

Subjects/Keywords: Nucleic acids

…194 C h ap ter 7.0 The stu d y of the nucleic acid interactions of zinc finger 4-7 region o… …transcription factors. 175 F ig u re 6.5 Am ino a d d sequence of the nucleic a d d binding d o m a in… …ribonuclease inhibitor XIX R N P : ribonucleoprotein rR N A : ribosom al nucleic acid S: Svedberg… …e am ino acids. T here is o n e series o f sev en consecutive se rin e-th reo n in e…

1 more image…

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Hamilton, T. (2017). Protein-nucleic acid interactions of Wilms' Tumor and TFIIIA zinc finger proteins. (Thesis). University of Victoria. Retrieved from http://hdl.handle.net/1828/8289

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Hamilton, Tatyana. “Protein-nucleic acid interactions of Wilms' Tumor and TFIIIA zinc finger proteins.” 2017. Thesis, University of Victoria. Accessed January 15, 2021. http://hdl.handle.net/1828/8289.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Hamilton, Tatyana. “Protein-nucleic acid interactions of Wilms' Tumor and TFIIIA zinc finger proteins.” 2017. Web. 15 Jan 2021.

Vancouver:

Hamilton T. Protein-nucleic acid interactions of Wilms' Tumor and TFIIIA zinc finger proteins. [Internet] [Thesis]. University of Victoria; 2017. [cited 2021 Jan 15]. Available from: http://hdl.handle.net/1828/8289.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Hamilton T. Protein-nucleic acid interactions of Wilms' Tumor and TFIIIA zinc finger proteins. [Thesis]. University of Victoria; 2017. Available from: http://hdl.handle.net/1828/8289

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Toronto

25. Arnold, Amy Elizabeth. Bioconjugate Strategies for Antisense Therapeutic Delivery to Glioblastoma Stem Cells.

Degree: PhD, 2020, University of Toronto

URL: http://hdl.handle.net/1807/100910

► Antisense therapeutics, including antisense oligonucleotides (AONs) and small interfering ribonucleic acids (siRNAs), are powerful tools for regulating genes, making them a promising therapy for diseases… (more)

▼ Antisense therapeutics, including antisense oligonucleotides (AONs) and small interfering ribonucleic acids (siRNAs), are powerful tools for regulating genes, making them a promising therapy for diseases such as cancer where oncogenic genes are over-expressed. The delivery of antisense therapeutics to target cells presents a significant challenge due to the many barriers a nucleic acid must face in order to reach the cytoplasm where it exerts its effects. In this thesis, I explored multiple strategies for delivery of AONs and siRNAs, focusing on targeting the desired cell population, inducing endocytosis, and facilitating endosomal escape. This was done within the context of glioblastoma (GBM), and specifically the glioblastoma stem cells (GSCs), an aggressive subpopulation of GBM cells that are involved in resistance, migration, and recurrence. Antisense oligonucleotides against a relevant GBM gene were conjugated to an antibody engineered to target CD44, a cell surface receptor which is highly expressed on GSCs. Using this system, we demonstrated functional targeting, endocytosis, and gene knockdown in the GSCs, leading to a morphological change in the cells. This represented the first time an antibody-oligonucleotide conjugate was used to target the GSC population. We were challenged with a lack of endosomal escape when using the antibody delivery platform, so we next looked at using a protein with a native endosomal escape mechanism to facilitate oligonucleotide delivery. For the second strategy, I conjugated attenuated diphtheria toxin (aDT), a protein which escapes the endolysosomal pathway, to siRNAs against relevant gene targets involved in GSC proliferation and invasion. Using this aDT-siRNA conjugate, we could downregulate genes of interest in the glioblastoma stem cells, leading to significant changes in cell viability and the invasive capacity of these cells. This is the first diphtheria toxin-based siRNA delivery vehicle and represents a platform technology for siRNA- and AON-based therapies. Advisors/Committee Members: Shoichet, Molly S, Chemistry.

Subjects/Keywords: Drug Delivery; Glioblastoma; Nucleic Acids; 0485

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Arnold, A. E. (2020). Bioconjugate Strategies for Antisense Therapeutic Delivery to Glioblastoma Stem Cells. (Doctoral Dissertation). University of Toronto. Retrieved from http://hdl.handle.net/1807/100910

Chicago Manual of Style (16^th Edition):

Arnold, Amy Elizabeth. “Bioconjugate Strategies for Antisense Therapeutic Delivery to Glioblastoma Stem Cells.” 2020. Doctoral Dissertation, University of Toronto. Accessed January 15, 2021. http://hdl.handle.net/1807/100910.

MLA Handbook (7^th Edition):

Arnold, Amy Elizabeth. “Bioconjugate Strategies for Antisense Therapeutic Delivery to Glioblastoma Stem Cells.” 2020. Web. 15 Jan 2021.

Vancouver:

Arnold AE. Bioconjugate Strategies for Antisense Therapeutic Delivery to Glioblastoma Stem Cells. [Internet] [Doctoral dissertation]. University of Toronto; 2020. [cited 2021 Jan 15]. Available from: http://hdl.handle.net/1807/100910.

Council of Science Editors:

Arnold AE. Bioconjugate Strategies for Antisense Therapeutic Delivery to Glioblastoma Stem Cells. [Doctoral Dissertation]. University of Toronto; 2020. Available from: http://hdl.handle.net/1807/100910

Oregon State University

26. Fu, Yingxian. Studies of time-resolved fluorescence spectroscopy and resolved absorption spectra of nucleic acid components.

Degree: PhD, Chemistry, 1993, Oregon State University

URL: http://hdl.handle.net/1957/35388

Subjects/Keywords: Nucleic acids – Spectra

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Fu, Y. (1993). Studies of time-resolved fluorescence spectroscopy and resolved absorption spectra of nucleic acid components. (Doctoral Dissertation). Oregon State University. Retrieved from http://hdl.handle.net/1957/35388

Chicago Manual of Style (16^th Edition):

Fu, Yingxian. “Studies of time-resolved fluorescence spectroscopy and resolved absorption spectra of nucleic acid components.” 1993. Doctoral Dissertation, Oregon State University. Accessed January 15, 2021. http://hdl.handle.net/1957/35388.

MLA Handbook (7^th Edition):

Fu, Yingxian. “Studies of time-resolved fluorescence spectroscopy and resolved absorption spectra of nucleic acid components.” 1993. Web. 15 Jan 2021.

Vancouver:

Fu Y. Studies of time-resolved fluorescence spectroscopy and resolved absorption spectra of nucleic acid components. [Internet] [Doctoral dissertation]. Oregon State University; 1993. [cited 2021 Jan 15]. Available from: http://hdl.handle.net/1957/35388.

Council of Science Editors:

Fu Y. Studies of time-resolved fluorescence spectroscopy and resolved absorption spectra of nucleic acid components. [Doctoral Dissertation]. Oregon State University; 1993. Available from: http://hdl.handle.net/1957/35388

Oregon State University

27. Winzeler, Elizabeth A. The effects of pH on the torsional flexibility of DNA bound to a nucleosome core particle.

Degree: MS, Biochemistry and Biophysics, 1990, Oregon State University

URL: http://hdl.handle.net/1957/37666

► The effects of pH on the torsional flexibility of DNA bound to a nucleosome core particle were investigated by studying the time-resolved fluorescence anisotropy decays… (more)

Subjects/Keywords: Nucleic acids – Analysis

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Winzeler, E. A. (1990). The effects of pH on the torsional flexibility of DNA bound to a nucleosome core particle. (Masters Thesis). Oregon State University. Retrieved from http://hdl.handle.net/1957/37666

Chicago Manual of Style (16^th Edition):

Winzeler, Elizabeth A. “The effects of pH on the torsional flexibility of DNA bound to a nucleosome core particle.” 1990. Masters Thesis, Oregon State University. Accessed January 15, 2021. http://hdl.handle.net/1957/37666.

MLA Handbook (7^th Edition):

Winzeler, Elizabeth A. “The effects of pH on the torsional flexibility of DNA bound to a nucleosome core particle.” 1990. Web. 15 Jan 2021.

Vancouver:

Winzeler EA. The effects of pH on the torsional flexibility of DNA bound to a nucleosome core particle. [Internet] [Masters thesis]. Oregon State University; 1990. [cited 2021 Jan 15]. Available from: http://hdl.handle.net/1957/37666.

Council of Science Editors:

Winzeler EA. The effects of pH on the torsional flexibility of DNA bound to a nucleosome core particle. [Masters Thesis]. Oregon State University; 1990. Available from: http://hdl.handle.net/1957/37666

University of Delaware

28. Foster, Abbygail A. Improving non-viral gene delivery: polymer carriers for spatial and temporal control of nucleic acid release.

Degree: PhD, University of Delaware, Department of Chemical Engineering, 2014, University of Delaware

URL: http://udspace.udel.edu/handle/19716/13381

► Gene therapy has garnered significant interest over the past few decades as an innovative approach for regulating protein expression towards the treatment of (i) genetic… (more)

▼ Gene therapy has garnered significant interest over the past few decades as an innovative approach for regulating protein expression towards the treatment of (i) genetic disorders including cystic fibrosis, severe combined immunodeficiency (SCID), and muscular dystrophy, (ii) acquired/infectious diseases such as Hepatitis and HIV/AIDS, and (iii) multiple varieties of cancer. Non-viral gene delivery approaches have received particular attention due their low toxicity, high nucleic acid storage capacity, and tailorability as compared to their viral counterparts. However, the efficiency and clinical realization of non-viral delivery vehicles has been limited by an incomplete understanding of their assembly, subcellular trafficking, and intracellular release mechanisms. Thus, the goal of this work was to develop strategies to understand and direct gene association and release using non-viral carriers, with the aim of improving current delivery platforms. Specific milestones of these studies include (1) the identification of the structural and functional consequences of fluorescent labeling of polymeric carriers for intracellular investigations, (2) the development of materials with tunable gene association and release capacity, as well as (3) the identification of novel non-viral approaches to address the functional requirements of gene transfer while promoting versatile preparation methods and facile cell-responsive delivery. A systematic investigation of the impact of fluorescent modification of an established polymer carrier, polyethylenimine, indicated that the incorporation of hydrophobic labeling moieties weakened gene association and promoted increased heterogeneity in polyplex structure. Additionally, cellular investigations identified extracellular aggregation and reduced polyplex uptake as a result of this routinely employed structural modification. In the design of controllable methods for promoting gene association and release, a polymeric delivery platform with light-responsive groups along the cationic segment of the polymer backbone was designed to provide controlled nucleic acid assembly and a user-defined method for intracellular release. Additionally, light-mediated polyplex destabilization demonstrated significant utility in siRNA delivery as observed through efficient siRNA release and enhanced protein silencing compared to a commercial Lipofectamine lipoplex. The demonstrated benefits of this stimuli-responsive delivery method motivated a cell-responsive approach to control gene association and release. A novel polymer-peptide conjugate with functional capabilities for controlled release, cell targeting, and endosomal escape was developed to address the numerous functional requirements in the gene delivery pathway. Ultimately, the clinical realization of gene therapies will rely on the development of methods to elucidate and control gene association and release along the delivery pathway. This will require the development of delivery vehicles whose physical and biochemical properties enable them to… Advisors/Committee Members: Sullivan, Millicent O..

Subjects/Keywords: Nucleic acids.; Polymers.; Controlled release technology.; Genes.

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Foster, A. A. (2014). Improving non-viral gene delivery: polymer carriers for spatial and temporal control of nucleic acid release. (Doctoral Dissertation). University of Delaware. Retrieved from http://udspace.udel.edu/handle/19716/13381

Chicago Manual of Style (16^th Edition):

Foster, Abbygail A. “Improving non-viral gene delivery: polymer carriers for spatial and temporal control of nucleic acid release.” 2014. Doctoral Dissertation, University of Delaware. Accessed January 15, 2021. http://udspace.udel.edu/handle/19716/13381.

MLA Handbook (7^th Edition):

Foster, Abbygail A. “Improving non-viral gene delivery: polymer carriers for spatial and temporal control of nucleic acid release.” 2014. Web. 15 Jan 2021.

Vancouver:

Foster AA. Improving non-viral gene delivery: polymer carriers for spatial and temporal control of nucleic acid release. [Internet] [Doctoral dissertation]. University of Delaware; 2014. [cited 2021 Jan 15]. Available from: http://udspace.udel.edu/handle/19716/13381.

Council of Science Editors:

Foster AA. Improving non-viral gene delivery: polymer carriers for spatial and temporal control of nucleic acid release. [Doctoral Dissertation]. University of Delaware; 2014. Available from: http://udspace.udel.edu/handle/19716/13381

University of Cape Town

29. Mew, Ronald Terence. Biochemical characterization of the nucleic acids of some human and animal viruses.

Degree: Image, Division of Chemical Pathology, 1982, University of Cape Town

URL: http://hdl.handle.net/11427/26479

► In Part I, the isolation and partial characterization of human polyomaviruses from a number of renal transplant patients is described. These isolates proved refractory to… (more)

▼ In Part I, the isolation and partial characterization of human polyomaviruses from a number of renal transplant patients is described. These isolates proved refractory to cell culture propagation by the methods used, and were thus extracted directly from large volumes of patient's urine. This approach has the advantage that the virus cannot undergo any possible genomic modification, as tends to occur during adaptation to cell culture. Human polyomavirus DNA is very susceptible to mutation during cell passage. Four isolates from different patients yielded sufficient DNA for limited restriction endonuclease characterization. Surprisingly, all four gave the same patterns with EcoRI, BamHI and HindIII. Two isolates that were also digested with PstI gave an identical pattern. These patterns are similar to, but distinct from, other strains of the human polyomavirus BK that have been described. Our isolates had a similar-sized genome to BK, but only 3 HindIII sites compared with 4 in the prototype, and 2 PstI sites compared with only 1 in the prototype. The quantity of DNA obtained directly from urine was usually very limited. In order to produce adequate DNA for complete analysis, viral DNA was recombined with· a bacterial vector (pBR322) and cloned into Escherichia coli strains HB101 and C600. Initially, the well-studied strain BK(MM) was successfully cloned. This clone was used to prepare radioactively-labelled DNA probes for the detection of BK-specific sequences in urine isolates and in subsequent recombinants with patient material. Such cloned material is easier to prepare in bulk than DNA from virus passaged in cell culture. Early attempts to clone DNA from clinical isolates failed, but BK-specific DNA from a patient (P.R.) has recently been cloned successfully. These clones are presently being used to investigate the differences in sequence between our isolates and the known strains of BK. It is hoped that this will shed light on the mechanisms of gene expression of these potentially oncogenic viruses. In Part II, the genomes of four rotaviruses were studied. "Simian agent 11" (SAll) and "offal agent" (OA) were cell culture-adapted strains, whereas "epizootic diarrhoea of infant mice virus" (EDIM) and "infantile gastroenteritis virus" (IGV) were isolated from stool specimens. Experiments were performed to confirm the double-stranded RNA (dsRNA) nature of the SAll genome. It ran at a characteristic density of l.595g/ml in caesium sulphate density gradients, and was resistant to DNase and RNase at high ionic strengths, but susceptible to RNase at low ionic strength. At the start of the project few or no polyacrylamide gel pictures of the nucleic acids of these viruses had been published, although it was known they resembled reovirus in consisting of segmented double-stranded RNA. Such pictures were obtained, and molecular weight estimations made by comparison with dsRNA markers of known MW from a cytoplasmic polyhedrosis virus (CPV) from Heliothis armigera (Harley, Rubenstein, Losman and Lutton, 1977. Virology 76:…

Subjects/Keywords: Viruses; Nucleic acids

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Mew, R. T. (1982). Biochemical characterization of the nucleic acids of some human and animal viruses. (Thesis). University of Cape Town. Retrieved from http://hdl.handle.net/11427/26479

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Mew, Ronald Terence. “Biochemical characterization of the nucleic acids of some human and animal viruses.” 1982. Thesis, University of Cape Town. Accessed January 15, 2021. http://hdl.handle.net/11427/26479.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Mew, Ronald Terence. “Biochemical characterization of the nucleic acids of some human and animal viruses.” 1982. Web. 15 Jan 2021.

Vancouver:

Mew RT. Biochemical characterization of the nucleic acids of some human and animal viruses. [Internet] [Thesis]. University of Cape Town; 1982. [cited 2021 Jan 15]. Available from: http://hdl.handle.net/11427/26479.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Mew RT. Biochemical characterization of the nucleic acids of some human and animal viruses. [Thesis]. University of Cape Town; 1982. Available from: http://hdl.handle.net/11427/26479

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Boston College

30. Theile, Christopher Stone. Synthesis of Cyclo, Ring Expanded, and Backbone Extended Nucleosides.

Degree: PhD, Chemistry, 2012, Boston College

URL: http://dlib.bc.edu/islandora/object/bc-ir:101931

► Nucleic acids are responsible for maintaining the biological information responsible for the activities of all known living organisms. Research of nucleic acids provides opportunities to… (more)

Subjects/Keywords: cyclonucleoside; DNA; Nucleic Acids; Nucleosides; nucleotides; RNA

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Theile, C. S. (2012). Synthesis of Cyclo, Ring Expanded, and Backbone Extended Nucleosides. (Doctoral Dissertation). Boston College. Retrieved from http://dlib.bc.edu/islandora/object/bc-ir:101931

Chicago Manual of Style (16^th Edition):

Theile, Christopher Stone. “Synthesis of Cyclo, Ring Expanded, and Backbone Extended Nucleosides.” 2012. Doctoral Dissertation, Boston College. Accessed January 15, 2021. http://dlib.bc.edu/islandora/object/bc-ir:101931.

MLA Handbook (7^th Edition):

Theile, Christopher Stone. “Synthesis of Cyclo, Ring Expanded, and Backbone Extended Nucleosides.” 2012. Web. 15 Jan 2021.

Vancouver:

Theile CS. Synthesis of Cyclo, Ring Expanded, and Backbone Extended Nucleosides. [Internet] [Doctoral dissertation]. Boston College; 2012. [cited 2021 Jan 15]. Available from: http://dlib.bc.edu/islandora/object/bc-ir:101931.

Council of Science Editors:

Theile CS. Synthesis of Cyclo, Ring Expanded, and Backbone Extended Nucleosides. [Doctoral Dissertation]. Boston College; 2012. Available from: http://dlib.bc.edu/islandora/object/bc-ir:101931

Open Access Theses and Dissertations