You searched for subject:(Nanobiotechnology).
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Deakin University
1.
Motilal Mathesh Shanmugam.
Enzyme architectonics on graphene oxides.
Degree: School of Life and Environmental Sciences, 2016, Deakin University
URL: http://hdl.handle.net/10536/DRO/DU:30089367 
► The thesis focuses on use of carbon nanoparticles, namely graphene oxides for studying the effect of hydrophobicity on enzyme structure and how they it influences…
(more)
▼ The thesis focuses on use of carbon nanoparticles, namely graphene oxides for studying the effect of hydrophobicity on enzyme structure and how they it influences the enzyme activity at molecular level. It was observed that controlling the hydrophobicity of the nanoparticle is a key towards modulating enzyme activity.
Advisors/Committee Members: Yang, Wenrong, Barrow, Colin.
Subjects/Keywords: Nanobiotechnology; Molecular electronics; Enzymes
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APA (6th Edition):
Shanmugam, M. M. (2016). Enzyme architectonics on graphene oxides. (Thesis). Deakin University. Retrieved from http://hdl.handle.net/10536/DRO/DU:30089367
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Shanmugam, Motilal Mathesh. “Enzyme architectonics on graphene oxides.” 2016. Thesis, Deakin University. Accessed January 15, 2021.
http://hdl.handle.net/10536/DRO/DU:30089367.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Shanmugam, Motilal Mathesh. “Enzyme architectonics on graphene oxides.” 2016. Web. 15 Jan 2021.
Vancouver:
Shanmugam MM. Enzyme architectonics on graphene oxides. [Internet] [Thesis]. Deakin University; 2016. [cited 2021 Jan 15].
Available from: http://hdl.handle.net/10536/DRO/DU:30089367.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Shanmugam MM. Enzyme architectonics on graphene oxides. [Thesis]. Deakin University; 2016. Available from: http://hdl.handle.net/10536/DRO/DU:30089367
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Rutgers University
2.
Bennett, Neal, 1989-.
Design of reprogrammed neuronal transplantation and nanobiomaterial-based microglial therapeutic technologies for management of Parkinson's disease.
Degree: PhD, Biomedical Engineering, 2016, Rutgers University
URL: https://rucore.libraries.rutgers.edu/rutgers-lib/51233/
► Parkinson's disease (PD) is a progressive neurodegenerative disorder marked by motor dysfunction, eventual cognitive impairment and dementia in advanced stages. These symptoms arise as a…
(more)
▼ Parkinson's disease (PD) is a progressive neurodegenerative disorder marked by motor dysfunction, eventual cognitive impairment and dementia in advanced stages. These symptoms arise as a result of decreased activity or death of dopaminergic (DA) neurons in the substantia nigra region of the brain, leading to dopamine depletion in the striatum disruption of neuronal circuitry in the basal ganglia. The current most common treatment for PD is levodopa, which can be converted into dopamine by surviving DA neurons, offering temporary relief of the motor dysfunction symptoms of PD. However, a key disadvantage of levodopa as a therapeutic strategy is that it does little to address the progression of PD and symptoms typically worsen as DA neurons continue to degenerate or die. Based on the a critical need for more comprehensive therapeutic approaches to PD that do more than relieve dopamine deficiency, but also disrupt the factors causing disease progression, the work in this dissertation focus on our efforts to address key aspects of Parkinson's disease pathology: neuronal degeneration, neuroinflammation, and synucleinopathy. To address neuronal degeneration, we investigated the potential for 3D fibrous synthetic substrates to support and transplant populations of human reprogrammed neurons into the brain. We found that fibrous substrate geometries could be tuned to shift reprogrammed cell populations towards either neuronal differentiation or maintenance of pluripotency. Microscale scaffolds generated from these fibrous substrates improved transplanted neuronal survival by at least an order of magnitude over traditional cell transplantation techniques. These proof-of-concept studies could be used to inform the future design of transplantable scaffolds supporting neurons reprogrammed to best address DA deficiency in PD. To address neuroinflammation and synucleinopathy, we examined the potential of microglia-targeting nanotherapeutics. We first identified scavenger receptors as a microglial receptor for α-synuclein (ASYN), a protein that forms characteristic protein aggregates and activates microglia in PD. We then designed nanoparticles targeting this interaction using synthetic amphiphilic scavenger receptor ligands. These amphiphilic molecules could reduce ASYN internalization and intracellular aggregation by microglia. We used nanoparticle constructs made using these synthetic ligands to target delivery of antioxidants to microglia, decreasing microglial activation in response to aggregated ASYN in vitro and in vivo. In summary, the studies described in this dissertation establish a valuable foundation for future therapeutic strategies addressing key features of PD pathophysiology and progression.
Advisors/Committee Members: Moghe, Prabhas V (chair), Pang, Zhiping (internal member), Grumet, Martin (internal member), Baum, Jean (outside member).
Subjects/Keywords: Parkinson's disease – Treatment; Nanobiotechnology
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APA (6th Edition):
Bennett, Neal, 1. (2016). Design of reprogrammed neuronal transplantation and nanobiomaterial-based microglial therapeutic technologies for management of Parkinson's disease. (Doctoral Dissertation). Rutgers University. Retrieved from https://rucore.libraries.rutgers.edu/rutgers-lib/51233/
Chicago Manual of Style (16th Edition):
Bennett, Neal, 1989-. “Design of reprogrammed neuronal transplantation and nanobiomaterial-based microglial therapeutic technologies for management of Parkinson's disease.” 2016. Doctoral Dissertation, Rutgers University. Accessed January 15, 2021.
https://rucore.libraries.rutgers.edu/rutgers-lib/51233/.
MLA Handbook (7th Edition):
Bennett, Neal, 1989-. “Design of reprogrammed neuronal transplantation and nanobiomaterial-based microglial therapeutic technologies for management of Parkinson's disease.” 2016. Web. 15 Jan 2021.
Vancouver:
Bennett, Neal 1. Design of reprogrammed neuronal transplantation and nanobiomaterial-based microglial therapeutic technologies for management of Parkinson's disease. [Internet] [Doctoral dissertation]. Rutgers University; 2016. [cited 2021 Jan 15].
Available from: https://rucore.libraries.rutgers.edu/rutgers-lib/51233/.
Council of Science Editors:
Bennett, Neal 1. Design of reprogrammed neuronal transplantation and nanobiomaterial-based microglial therapeutic technologies for management of Parkinson's disease. [Doctoral Dissertation]. Rutgers University; 2016. Available from: https://rucore.libraries.rutgers.edu/rutgers-lib/51233/
Anna University
3.
Padmavathy, B.
Development of nanobiosensors for diagnostics of
bacterial pathogens; -.
Degree: Biotechnology, 2013, Anna University
URL: http://shodhganga.inflibnet.ac.in/handle/10603/24765
► Infectious diseases are posing to be a major healthcare problem newlinethroughout the world The contagious nature of these diseases makes it newlineimperative for early diagnosis…
(more)
▼ Infectious diseases are posing to be a major
healthcare problem newlinethroughout the world The contagious
nature of these diseases makes it newlineimperative for early
diagnosis of the microbial cause of infection to prevent newlinethe
spread of epidemics Conventional standard diagnostic methods have
newlinelimitations including laborious sample preparation poor
sensitivity and newlinedelayed data readout Hence rapid field
deployable diagnostic modalities are newlinein urgent need to
combat the problems in identification of food contamination
newlinedirect pathogen detection from clinical samples etc
Nanobiotechnology based newlineapproach present a great opportunity
to develop fast accurate and cost newlineeffective diagnostics The
nanoparticles posses unique optical electrical and newlinemagnetic
properties based on their core materials that renders them as a
newlinesensitive signaling probes Also nanoparticles are amenable
to surface newlinemodifications and biofunctionalization which can
be exploited as target newlinespecific and sensitive signaling tool
in the development of diagnostic newlinebiosensors newlineThis
thesis aims to develop and compare various surface
newlinechemistries biofunctionalization and detection strategies
using nanoparticles newlinefor invitro diagnostics of infectious
diseases suitable for developing newlinecountries To realize these
objectives the magnetic nanoparticles gold newlinenanoparticles and
dye doped silica nanoparticles were employed for nucleic
newlineacid and antibody based detection of bacterial pathogens The
magnetic newlinenanoparticles were covalently attached to antibody
specific to the target newlinepathogen These immunomagnetic
nanoparticles after incubation with newlinecontaminated food
samples form a complex with the target pathogen A newlinesimple
magnetic field can be applied to localize and collect the target
newlinepathogen while the other contaminants can be easily washed
away The newlineimmunomagnetic nanoparticles based approach thus
speeds up sample newlinepreparation from low bacterial load samples
to yield sufficient high purity newlineDNA required for PCR based
detection newline newline newline newline newline newline newline
newline newline newline newline newline newline
newline
-
Advisors/Committee Members: Jaffar Ali, B M.
Subjects/Keywords: Bacterial Pathogens; contamination; Diagnostics; Nanobiosensors; Nanobiotechnology
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APA (6th Edition):
Padmavathy, B. (2013). Development of nanobiosensors for diagnostics of
bacterial pathogens; -. (Thesis). Anna University. Retrieved from http://shodhganga.inflibnet.ac.in/handle/10603/24765
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Padmavathy, B. “Development of nanobiosensors for diagnostics of
bacterial pathogens; -.” 2013. Thesis, Anna University. Accessed January 15, 2021.
http://shodhganga.inflibnet.ac.in/handle/10603/24765.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Padmavathy, B. “Development of nanobiosensors for diagnostics of
bacterial pathogens; -.” 2013. Web. 15 Jan 2021.
Vancouver:
Padmavathy B. Development of nanobiosensors for diagnostics of
bacterial pathogens; -. [Internet] [Thesis]. Anna University; 2013. [cited 2021 Jan 15].
Available from: http://shodhganga.inflibnet.ac.in/handle/10603/24765.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Padmavathy B. Development of nanobiosensors for diagnostics of
bacterial pathogens; -. [Thesis]. Anna University; 2013. Available from: http://shodhganga.inflibnet.ac.in/handle/10603/24765
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Massey University
4.
Sattar, Sadia.
Filamentous phage-derived nano-rods for applications in diagnostics and vaccines.
Degree: PhD, Biochemistry, 2013, Massey University
URL: http://hdl.handle.net/10179/5539
► Filamentous bacteriophage, as their name indicates are filament-like bacterial viruses. The F-pilus-specific filamentous phage of Escherichia coli, Ff (f1, M13 and fd) are resistant to…
(more)
▼ Filamentous bacteriophage, as their name indicates are filament-like bacterial viruses. The F-pilus-specific filamentous phage of Escherichia coli, Ff (f1, M13 and fd) are resistant to heat, pH extremes and detergents. Their structural properties and amenability to engineering using recombinant DNA technology have enabled their extensive use in modern biotechnology. For example, Ff can be functionalized by displaying up to five different proteins and peptides on their surface. Ff phage have been successfully employed in diagnostic devices. Moreover, direct use as antigen-carriers is also a subject of interest in vaccine development. However, use of Ff-phage vaccines and in the at-home diagnostic devices is controversial, mainly because of their ability to replicate in gut E. coli, and possibility of mobilization and horizontal gene transfer of antibiotic resistance or virulence factor-encoding genes transfer among the gut and environmental bacteria. Moreover, the large length-to-diameter ratio of the virion (1000 nm x 6 nm) impairs diffusion of filamentous phage through complex matrices and could restrict use of filamentous phage in lateral flow diagnostic devices.
To overcome both of these problems we have constructed much shorter, rod-like functionalized particles (50 nm x 6 nm), named “Ff-nano”, which do not carry any genes. The properties of these short particles were investigated, showing that they have superior resistance to heating in the presence of ionic detergent sodium dodecyl sulphate (SDS) in comparison to the full-length phage of the same virion composition. The Ff-nano particles displaying a bacterial Fibronectin-Binding (FnB) protein as fusion to virion protein pIII, localized in five copies at one of the two ends of the virion, were produced and purified. These functionalized nanorods were tested in two applications: as detector particles in a dip-stick-type lateral flow device and as antigen carrier in a vaccine trial. The FnB-displaying nanorods were able to quantitatively detect fibronectin in solution. In the vaccine trial, the Ff-nano particles elicited a weak response to the FnB displayed at a low-copy-number at the nanorod end. In contrast, the response to the major protein pVIII was strong, indicating that the multi-copy display of antigenic peptides along the rod, as fusion to the major coat protein pVIII, is required for using the Ff-nano effectively as vaccine carriers.
Subjects/Keywords: Bacteriophages;
Biotechnology;
Vaccine development;
Diagnostics;
Nanobiotechnology
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APA (6th Edition):
Sattar, S. (2013). Filamentous phage-derived nano-rods for applications in diagnostics and vaccines. (Doctoral Dissertation). Massey University. Retrieved from http://hdl.handle.net/10179/5539
Chicago Manual of Style (16th Edition):
Sattar, Sadia. “Filamentous phage-derived nano-rods for applications in diagnostics and vaccines.” 2013. Doctoral Dissertation, Massey University. Accessed January 15, 2021.
http://hdl.handle.net/10179/5539.
MLA Handbook (7th Edition):
Sattar, Sadia. “Filamentous phage-derived nano-rods for applications in diagnostics and vaccines.” 2013. Web. 15 Jan 2021.
Vancouver:
Sattar S. Filamentous phage-derived nano-rods for applications in diagnostics and vaccines. [Internet] [Doctoral dissertation]. Massey University; 2013. [cited 2021 Jan 15].
Available from: http://hdl.handle.net/10179/5539.
Council of Science Editors:
Sattar S. Filamentous phage-derived nano-rods for applications in diagnostics and vaccines. [Doctoral Dissertation]. Massey University; 2013. Available from: http://hdl.handle.net/10179/5539
Michigan State University
5.
Song, Bo.
Real-time multimodal sensing in nano/bio environment.
Degree: 2016, Michigan State University
URL: http://etd.lib.msu.edu/islandora/object/etd:4488
► Thesis Ph. D. Michigan State University. Electrical Engineering 2016
As a sensing device in nano-scale, scanning probe microscopy (SPM) is a powerful tool for exploring…
(more)
▼ Thesis Ph. D. Michigan State University. Electrical Engineering 2016
As a sensing device in nano-scale, scanning probe microscopy (SPM) is a powerful tool for exploring nano world. Nevertheless two fundamental problems tackle the development and application of SPM based imaging and measurement: slow imaging/measurement speed and inaccuracy of motion or position control. Usually, SPM imaging/properties measuring speed is too slow to capture a dynamic observation on sample surface. In addition, Both SPM imaging and properties measurement always experience positioning inaccuracy problems caused by hysteresis and creep of the piezo scanner. This dissertation will try to solve these issues and proposed a SPM based real-time multimodal sensing system which can be used in nano/bio environment. First, a compressive sensing based video rate fast SPM imaging system is shown as an efficient method to dynamically capture the sample surface change with the imaging speed 1.5 frame/s with the scan size of 500 nm * 500 nm. Besides topography imaging, a new additional modal of SPM: vibration mode, will be introduced, and it is developed by us to investigate the subsurface mechanical properties of the elastic sample such as cells and bacteria. A followed up study of enzymatic hydrolysis will demonstrate the ability of in situ observation of single molecule event using video rate SPM. After that we will introduce another modal of this SPM sensing system: accurate electrical properties measurement. In this electrical properties measurement mode, a compressive feedbacks based non-vector space control approach is proposed in order to improve the accuracy of SPM based nanomanipulations. Instead of sensors, the local images are used as both the input and feedback of a non-vector space closed-loop controller. A followed up study will also be introduced to shown the important role of non-vector space control in the study of conductivity distribution of multi-wall carbon nanotubes. At the end of this dissertation, some future work will be also proposed to fulfill the development and validation of this real-time multimodal sensing system.
Description based on online resource;
Advisors/Committee Members: Dong, Lixin, Khalil, Hassan K, Wang, Donna, Tan, Xiaobo.
Subjects/Keywords: Scanning probe microscopy; Nanobiotechnology; Electrical engineering; Nanotechnology
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APA (6th Edition):
Song, B. (2016). Real-time multimodal sensing in nano/bio environment. (Thesis). Michigan State University. Retrieved from http://etd.lib.msu.edu/islandora/object/etd:4488
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Song, Bo. “Real-time multimodal sensing in nano/bio environment.” 2016. Thesis, Michigan State University. Accessed January 15, 2021.
http://etd.lib.msu.edu/islandora/object/etd:4488.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Song, Bo. “Real-time multimodal sensing in nano/bio environment.” 2016. Web. 15 Jan 2021.
Vancouver:
Song B. Real-time multimodal sensing in nano/bio environment. [Internet] [Thesis]. Michigan State University; 2016. [cited 2021 Jan 15].
Available from: http://etd.lib.msu.edu/islandora/object/etd:4488.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Song B. Real-time multimodal sensing in nano/bio environment. [Thesis]. Michigan State University; 2016. Available from: http://etd.lib.msu.edu/islandora/object/etd:4488
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
6.
Sajimol Augustine, M.
Investigations on novel polymer nano composites and bio-compatible manganese doped ZnS nanocrystals for photonic and bio-imaging applications.
Degree: Physics, 2012, Cochin University of Science and Technology
URL: http://dyuthi.cusat.ac.in/purl/3691
► Fluorescence is a powerful tool in biological research, the relevance of which relies greatly on the availability of sensitive and selective fluorescent probes. Nanometer sized…
(more)
▼ Fluorescence is a powerful tool in biological research, the relevance of
which relies greatly on the availability of sensitive and selective fluorescent
probes. Nanometer sized fluorescent semiconductor materials have attracted considerable attention in recent years due to the high luminescence intensity, low
photobleaching, large Stokes’ shift and high photochemical stability. The optical
and spectroscopic features of nanoparticles make them very convincing
alternatives to traditional fluorophores in a range of applications. Efficient surface
capping agents make these nanocrystals bio-compatible. They can provide a novel
platform on which many biomolecules such as DNA, RNA and proteins can be
covalently linked. In the second phase of the present work, bio-compatible,
fluorescent, manganese doped ZnS (ZnS:Mn) nanocrystals suitable for bioimaging
applications have been developed and their cytocompatibility has been
assessed. Functionalization of ZnS:Mn nanocrystals by safe materials results in
considerable reduction of toxicity and allows conjugation with specific
biomolecules. The highly fluorescent, bio-compatible and water- dispersible
ZnS:Mn nanocrystals are found to be ideal fluorescent probes for biological
labeling
Cochin University of Science and
Technology
Subjects/Keywords: Nanoscience; Nanotechnology; Nanobiotechnology; Nanoscale materials; Zinc Oxide/Polyvinyl Alcohol
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APA (6th Edition):
Sajimol Augustine, M. (2012). Investigations on novel polymer nano composites and bio-compatible manganese doped ZnS nanocrystals for photonic and bio-imaging applications. (Thesis). Cochin University of Science and Technology. Retrieved from http://dyuthi.cusat.ac.in/purl/3691
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Sajimol Augustine, M. “Investigations on novel polymer nano composites and bio-compatible manganese doped ZnS nanocrystals for photonic and bio-imaging applications.” 2012. Thesis, Cochin University of Science and Technology. Accessed January 15, 2021.
http://dyuthi.cusat.ac.in/purl/3691.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Sajimol Augustine, M. “Investigations on novel polymer nano composites and bio-compatible manganese doped ZnS nanocrystals for photonic and bio-imaging applications.” 2012. Web. 15 Jan 2021.
Vancouver:
Sajimol Augustine M. Investigations on novel polymer nano composites and bio-compatible manganese doped ZnS nanocrystals for photonic and bio-imaging applications. [Internet] [Thesis]. Cochin University of Science and Technology; 2012. [cited 2021 Jan 15].
Available from: http://dyuthi.cusat.ac.in/purl/3691.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Sajimol Augustine M. Investigations on novel polymer nano composites and bio-compatible manganese doped ZnS nanocrystals for photonic and bio-imaging applications. [Thesis]. Cochin University of Science and Technology; 2012. Available from: http://dyuthi.cusat.ac.in/purl/3691
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Ryerson University
7.
Yohan, Darren.
Gold nanoparticle transport in multilayered cell cultures.
Degree: 2015, Ryerson University
URL: https://digital.library.ryerson.ca/islandora/object/RULA%3A3715
► Gold nanoparticles (GNPs) possess a number of useful characteristics that have catapulted them into the mainstream of cancer research. Their optical properties enable them to…
(more)
▼ Gold nanoparticles (GNPs) possess a number of useful characteristics that have catapulted them into the mainstream of cancer research. Their optical properties enable them to be used in photodynamic and photothermal therapy as well as contrast agents in photoacoustic imaging. In addition, the ability to bind ligands to the GNP surface has made them valuable bio-markeraware drug carriers. But the effectiveness of any cancer fighting tool relies on homogenous distribution and penetration throughout the tumor, and the uptake and transport dynamics of GNPs has previously been held to monolayer cell models. In this work, multicellular layers (MCLs) are used as a solid tumor model to measure the penetration and uptake of GNPs in tumor tissue. MCLs offer a unique way to bridge the gap between in vitro single-layer cell models and the in vivo tumor. The effects of increased cell-to-cell connections, extracellular matrix and tumor characteristics are investigated to deliver new insights into the transport of GNPs in tissue.
Advisors/Committee Members: Ryerson University (Degree grantor).
Subjects/Keywords: Cancer – Research; Nanoparticles – Research; Gold; Nanomedicine.; Nanobiotechnology; Biotechnology – Materials
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APA (6th Edition):
Yohan, D. (2015). Gold nanoparticle transport in multilayered cell cultures. (Thesis). Ryerson University. Retrieved from https://digital.library.ryerson.ca/islandora/object/RULA%3A3715
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Yohan, Darren. “Gold nanoparticle transport in multilayered cell cultures.” 2015. Thesis, Ryerson University. Accessed January 15, 2021.
https://digital.library.ryerson.ca/islandora/object/RULA%3A3715.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Yohan, Darren. “Gold nanoparticle transport in multilayered cell cultures.” 2015. Web. 15 Jan 2021.
Vancouver:
Yohan D. Gold nanoparticle transport in multilayered cell cultures. [Internet] [Thesis]. Ryerson University; 2015. [cited 2021 Jan 15].
Available from: https://digital.library.ryerson.ca/islandora/object/RULA%3A3715.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Yohan D. Gold nanoparticle transport in multilayered cell cultures. [Thesis]. Ryerson University; 2015. Available from: https://digital.library.ryerson.ca/islandora/object/RULA%3A3715
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Columbia University
8.
Fazio, Teresa.
Nanolithographic Control of Double-Stranded DNA at the Single-Molecule Level.
Degree: 2012, Columbia University
URL: https://doi.org/10.7916/D8SQ9C1T
► This thesis describes methods for constructing nanopatterned surfaces to array DNA. These surfaces enable direct observation of heretofore-unseen single-molecule reactions, eliminating bulk effects and enabling…
(more)
▼ This thesis describes methods for constructing nanopatterned surfaces to array DNA. These surfaces enable direct observation of heretofore-unseen single-molecule reactions, eliminating bulk effects and enabling scientists to examine DNA mismatch repair and replication, including the first direct visualization of proteins binding to a target mismatch. This also facilitates directed self-organization of nanoscale features on a patterned substrate using DNA as an assembly tool.
To make techniques for single-molecule visualization of biological processes more accessible, we have developed a novel technology called "DNA curtains," in which a combination of fluid lipid bilayers, nanofabricated barriers to lipid diffusion, and hydrodynamic flow can organize lipid-tethered DNA molecules into dened patterns on the surface of a microfluidic sample chamber.
Using DNA curtains, aligned DNA molecules can be visualized by total internal reflection fluorescence microscopy, allowing simultaneous observation of hundreds of individual molecules within a field-of-view. Ultimately, this results in a 100X improvement in experimental throughput, and a corresponding increase in statistically signicant amounts of data.
We also demonstrate site-specific labeling of DNA using DNA analogues, such as peptide nucleic acid (PNA), locked nucleic acid (LNA), and techniques such as nick-translation. Through PNA invasion, labeled DNA was self-assembled in arrays on surfaces and tagged with gold nanoparticles. In this work, DNA formed a template to self-assemble a nanoparticle in between nanoimprinted AuPd dots. Surface-based self-assembly methods offer potential for DNA employment in bottom-up construction of nanoscale arrays. This offers further proof that DNA can be useful in directed self-assembly of nanoscale architectures.
Subjects/Keywords: Materials science; Nanolithography; Nanobiotechnology; DNA – Analysis; Nanostructured materials
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APA (6th Edition):
Fazio, T. (2012). Nanolithographic Control of Double-Stranded DNA at the Single-Molecule Level. (Doctoral Dissertation). Columbia University. Retrieved from https://doi.org/10.7916/D8SQ9C1T
Chicago Manual of Style (16th Edition):
Fazio, Teresa. “Nanolithographic Control of Double-Stranded DNA at the Single-Molecule Level.” 2012. Doctoral Dissertation, Columbia University. Accessed January 15, 2021.
https://doi.org/10.7916/D8SQ9C1T.
MLA Handbook (7th Edition):
Fazio, Teresa. “Nanolithographic Control of Double-Stranded DNA at the Single-Molecule Level.” 2012. Web. 15 Jan 2021.
Vancouver:
Fazio T. Nanolithographic Control of Double-Stranded DNA at the Single-Molecule Level. [Internet] [Doctoral dissertation]. Columbia University; 2012. [cited 2021 Jan 15].
Available from: https://doi.org/10.7916/D8SQ9C1T.
Council of Science Editors:
Fazio T. Nanolithographic Control of Double-Stranded DNA at the Single-Molecule Level. [Doctoral Dissertation]. Columbia University; 2012. Available from: https://doi.org/10.7916/D8SQ9C1T
Hong Kong University of Science and Technology
9.
Wang, Wen.
Self-assembly of complex nanoarchitectures from multiple-stranded DNA tiles and DNA nanodevices for cell biology.
Degree: 2014, Hong Kong University of Science and Technology
URL: http://repository.ust.hk/ir/Record/1783.1-88755
;
https://doi.org/10.14711/thesis-b1432230
;
http://repository.ust.hk/ir/bitstream/1783.1-88755/1/th_redirect.html
► Programmed self-assembly of nucleic acid (DNA and RNA) provides a powerful approach for constructing a variety of remarkable nanoscale structures and devices. Because of its…
(more)
▼ Programmed self-assembly of nucleic acid (DNA and RNA) provides a powerful approach for constructing a variety of remarkable nanoscale structures and devices. Because of its self-assembling nature, DNA is an excellent candidate for creating predictable and programmable nanoarchitectures. With the ability to combine designed DNA-branched junctions with sticky-end cohesion, structural DNA nanotechnology (SDN) has developed over the past 30 years. A wide range of multidimensional nanoarchitectures has been constructed, providing all sorts of potential applications. In this thesis, we first describe the design and self-assembly of complex nanoarchitectures from multiple-stranded DNA motifs. Unpurified strands without perfect stoichiometry were mixed to form finite-sized structures. By utilizing legacy double crossover (DX) motifs with different domain length designs, we successfully constructed the corresponding 2D arrays, and characterized the structure by atomic force microscopy (AFM). A variety of wire-frame structures with different mesh sizes were also successfully built with three-arm junction, four-arm junction and hexagonal motifs. Secondly, with a strong interest in the cellular applications of DNA nanoarchitectures, we designed and constructed a DNA-based, pH-responsive nanocarrier system for targeted drug delivery. We used the PSMA A10 RNA aptamer to specifically target human prostate LNCaP cells, and employed i-motif as the pH-responsive unit. The nanocarriers were characterized by a particle size analyzer and scanning electron microscopy (SEM). The in vitro pH-responsive drug release was verified by fluorescence spectroscopy. We also demonstrated the specific targeting ability of the nanocarrier system by anti-proliferation assay, as well as cellular uptake visualized by confocal microscopy. Finally, we developed a DNA-based nanothermometer that utilized triple fluorescent labeled oligonucleotides. Combining FRET effect and temperature-responsive DNA, this thermometer could measure the temperature increases in living Hela cells. The in vitro and in vivo temperature-responsiveness were confirmed by fluorescence spectrometry and confocal microscopy.
Subjects/Keywords: Nanostructured materials
; Design and construction
; Nanobiotechnology
; Self-assembly (Chemistry)
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APA (6th Edition):
Wang, W. (2014). Self-assembly of complex nanoarchitectures from multiple-stranded DNA tiles and DNA nanodevices for cell biology. (Thesis). Hong Kong University of Science and Technology. Retrieved from http://repository.ust.hk/ir/Record/1783.1-88755 ; https://doi.org/10.14711/thesis-b1432230 ; http://repository.ust.hk/ir/bitstream/1783.1-88755/1/th_redirect.html
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Wang, Wen. “Self-assembly of complex nanoarchitectures from multiple-stranded DNA tiles and DNA nanodevices for cell biology.” 2014. Thesis, Hong Kong University of Science and Technology. Accessed January 15, 2021.
http://repository.ust.hk/ir/Record/1783.1-88755 ; https://doi.org/10.14711/thesis-b1432230 ; http://repository.ust.hk/ir/bitstream/1783.1-88755/1/th_redirect.html.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Wang, Wen. “Self-assembly of complex nanoarchitectures from multiple-stranded DNA tiles and DNA nanodevices for cell biology.” 2014. Web. 15 Jan 2021.
Vancouver:
Wang W. Self-assembly of complex nanoarchitectures from multiple-stranded DNA tiles and DNA nanodevices for cell biology. [Internet] [Thesis]. Hong Kong University of Science and Technology; 2014. [cited 2021 Jan 15].
Available from: http://repository.ust.hk/ir/Record/1783.1-88755 ; https://doi.org/10.14711/thesis-b1432230 ; http://repository.ust.hk/ir/bitstream/1783.1-88755/1/th_redirect.html.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Wang W. Self-assembly of complex nanoarchitectures from multiple-stranded DNA tiles and DNA nanodevices for cell biology. [Thesis]. Hong Kong University of Science and Technology; 2014. Available from: http://repository.ust.hk/ir/Record/1783.1-88755 ; https://doi.org/10.14711/thesis-b1432230 ; http://repository.ust.hk/ir/bitstream/1783.1-88755/1/th_redirect.html
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
University of Illinois – Urbana-Champaign
10.
Carr, Rogan C.
Modeling the interface between biological and synthetic components in hybrid nanosystems.
Degree: PhD, 0240, 2012, University of Illinois – Urbana-Champaign
URL: http://hdl.handle.net/2142/29413
► The fusion of biology and nanotechnology holds amazing promise for revolutionizing medicine and personal technology. In order to take advantage of the great feats of…
(more)
▼ The fusion of biology and nanotechnology holds amazing promise for revolutionizing medicine and personal technology. In order to take advantage of the great feats of engineering coming out of these fields, there needs to be theories and computational tools capable of describing the interface between the pristine and ordered world of precision electronics and the hot, wet, and stochastic world of biology. The success of these technologies will depend on our abilities to design and optimize interactions of biomolecules and solid-state materials down to the atomic scale. In my research at the University of Illinois, I have used molecular dynamics (MD) as a tool to describe the atomic-scale interactions driving the function of biomolecules and their interface with solid-state devices, and I have sought to use it as a starting point to create new methods for modeling, designing, and optimizing these interactions. In my dissertation, I show the methodologies that I use in my work and the range of possibilities that they present for researchers in the ???eld, and I present my research on the modeling of the interface between biological and synthetic materials in (1) immunosurfaces used for detection of live bacteria; (2) protein transport through a nanochannel; (3) deriving the Langmuir constant for adsorption for small, organic molecules on synthetic surfaces; (4) creating a multiscale model for transport in micro- and nanofluidic devices; (5) synthetic analogs of biological ion channels. It is my hope that the research that I have performed in my doctoral studies here at the University of Illinois at Urbana-Champaign will be a template for future research and interdisciplinary science. The work I have done here has shown the possibilities of the MD method for studying the physical interface of biological and synthetic components, and I have developed new techniques that can be used by researchers in field to further the science and engineering of bionanotechnology and
nanobiotechnology.
Advisors/Committee Members: Aksimentiev, Aleksei (advisor), Clegg, Robert M. (Committee Chair), Aksimentiev, Aleksei (committee member), Olson, Luke N. (committee member), Stack, John D. (committee member).
Subjects/Keywords: Molecular Dynamics; Bionanotechnology; Nanobiotechnology; Nanofluidics; Adsorption; Langmuir Isotherm; Nanochannel; Polyoxometalates; Immunosurfaces
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APA (6th Edition):
Carr, R. C. (2012). Modeling the interface between biological and synthetic components in hybrid nanosystems. (Doctoral Dissertation). University of Illinois – Urbana-Champaign. Retrieved from http://hdl.handle.net/2142/29413
Chicago Manual of Style (16th Edition):
Carr, Rogan C. “Modeling the interface between biological and synthetic components in hybrid nanosystems.” 2012. Doctoral Dissertation, University of Illinois – Urbana-Champaign. Accessed January 15, 2021.
http://hdl.handle.net/2142/29413.
MLA Handbook (7th Edition):
Carr, Rogan C. “Modeling the interface between biological and synthetic components in hybrid nanosystems.” 2012. Web. 15 Jan 2021.
Vancouver:
Carr RC. Modeling the interface between biological and synthetic components in hybrid nanosystems. [Internet] [Doctoral dissertation]. University of Illinois – Urbana-Champaign; 2012. [cited 2021 Jan 15].
Available from: http://hdl.handle.net/2142/29413.
Council of Science Editors:
Carr RC. Modeling the interface between biological and synthetic components in hybrid nanosystems. [Doctoral Dissertation]. University of Illinois – Urbana-Champaign; 2012. Available from: http://hdl.handle.net/2142/29413
University of Waterloo
11.
Bedford, Erin.
Gold Surface Nanostructuring for Separation and Sensing of Biomolecules.
Degree: 2016, University of Waterloo
URL: http://hdl.handle.net/10012/11107
► Detecting biomolecules in physiological environments is critical to health care and environmental monitoring. In this work, we study and use gold surfaces for biomolecule detection…
(more)
▼ Detecting biomolecules in physiological environments is critical to health care and environmental monitoring. In this work, we study and use gold surfaces for biomolecule detection while incorporating nanoscale components—specifically, self-assembled monolayers (SAMs) of alkanethiols and gold nanostructured shells—with the goal of improving biomolecule detection methods. Using SAMs to functionalize gold surfaces can offer control over biomolecule binding density and orientation while still keeping the biomolecules near the sensing surface. Using surface IR spectroscopy, x-ray photoelectron spectroscopy (XPS), and density functional theory (DFT) modeling, we found that SAMs of short-chain and long-chain amine-terminated alkanethiols on gold had different sulphur binding environments. We also found that protein binding and recognition on the two different SAMs varied with SAM chain length and was also influenced by the presence of a cross-linker. In the second part of this work, we synthesized gold nanostructured shells on magnetic particles for combined separation and detection of biomolecules. We demonstrated their use as substrates for surface-enhanced Raman spectroscopy (SERS) As a proof-of-concept, we demonstrated the use of these particles to detect oligonucleotide binding and hybridization with SERS using a Raman-tagged oligonucleotide hairpin probe.
Subjects/Keywords: nanobiotechnology; nanoparticles; gold; self-assembled monolayers; biosensors; surface-enhanced Raman spectroscopy
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APA (6th Edition):
Bedford, E. (2016). Gold Surface Nanostructuring for Separation and Sensing of Biomolecules. (Thesis). University of Waterloo. Retrieved from http://hdl.handle.net/10012/11107
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Bedford, Erin. “Gold Surface Nanostructuring for Separation and Sensing of Biomolecules.” 2016. Thesis, University of Waterloo. Accessed January 15, 2021.
http://hdl.handle.net/10012/11107.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Bedford, Erin. “Gold Surface Nanostructuring for Separation and Sensing of Biomolecules.” 2016. Web. 15 Jan 2021.
Vancouver:
Bedford E. Gold Surface Nanostructuring for Separation and Sensing of Biomolecules. [Internet] [Thesis]. University of Waterloo; 2016. [cited 2021 Jan 15].
Available from: http://hdl.handle.net/10012/11107.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Bedford E. Gold Surface Nanostructuring for Separation and Sensing of Biomolecules. [Thesis]. University of Waterloo; 2016. Available from: http://hdl.handle.net/10012/11107
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Tampere University
12.
Leppiniemi, Jenni.
The Development of Novel Biomolecular Tools Based on Avidins, DNA and Chitosan
.
Degree: 2014, Tampere University
URL: https://trepo.tuni.fi/handle/10024/95261
► Nanobioteknologia ja bionanoteknologia ovat suhteellisen uusia, nopeasti kehittyviä tieteenaloja, jotka hyödyntävät biomolekyylejä. Biomolekyylien luonnolliset ominaisuudet ja toiminta täytyy tuntea hyvin, jotta niitä voidaan hyödyntää moderneissa…
(more)
▼ Nanobioteknologia ja bionanoteknologia ovat suhteellisen uusia, nopeasti kehittyviä tieteenaloja, jotka hyödyntävät biomolekyylejä. Biomolekyylien luonnolliset ominaisuudet ja toiminta täytyy tuntea hyvin, jotta niitä voidaan hyödyntää moderneissa teknisissä sovelluksissa. Biomolekyylien ominaisuuksia täytyy myös usein muokata joko geneettisesti tai kemiallisesti, jotta ne soveltuvat tiettyyn käyttökohteeseen.
Tämän tutkimuksen tavoitteena oli karakterisoida ja muokata biomolekyylien ominaisuuksia, jotta niistä voitaisiin valmistaa molekyylityökaluja nanobioteknologian ja bionanoteknologian tarpeisiin. Avidiini-proteiinit valittiin muokattaviksi sen vuoksi, että niitä käytetään jo laajalti bioteknologian sovelluksissa, sillä ne sitovat poikkeuksellisen voimakkaasti pientä vitamiini-molekyyliä, D-biotiinia (Kd ~10-15 M). Tässä työssä muokattiin aiemmin kehitetyn kaksoisketjuavidiinin (dcAvd) toista ligandin sitoutumispaikkaa muodostamaan kovalentin sidoksen ligandin kanssa. Näin aikaansaatu uusi proteiini, dcAvd-Cys, kykenee sitoutumaan kahdenlaisten molekyylien, tioli-reaktiivisten sekä biotinyloitujen molekyylien kanssa samanaikaisesti, joten sitä voitaisiin hyödyntää molekyylien hallitussa immobilisoinnissa.
Työssä määritettiin kahden avidiinin kiderakenteet suurella tarkkuudella. Rakenteen, biokemiallisen ja biofysikaalisen karakterisoinnin perusteella näiden proteiinien käyttäytyminen poikkeaa aiemmin tutkituista avidiineista. Tetrameerisen villityypin bradavidiinin C-terminaaliset aminohappotähteet vuorovaikuttavat viereisen monomeerin ligandinsitomistaskun kanssa, käyttäytyen kuten monomeerien välinen sisäinen ligandi. Tämä havainto johti bradavidiini-spesifisen Brad-tag:n kehittämiseen, ja Brad-tag:n osoitettiin olevan käyttökelpoinen affiniteettikahva (Kd ~2,5 × 10-5 M). Suurin osa tutkituista (strept)avidiineista on tetrameerisia tai dimeerisiä proteiineja, kun taas bradavidiini II:n oligomeerisuusaste osoittautui vaihtelevan ympäröivien olosuhteiden mukaan. Bradavidiini II:n heikkoa oligomerisoitumispyrkimystä voitaisiin hyödyntää fuusioproteiinien kehittämisessä.
Avidiinien ohella tutkittiin kahta erilaista biomolekyylien muodostamaa kompleksia. Ensimmäiseksi hyödynnettiin deoksiribonukleiinihappojen (DNA) ominaisuuksia kehitettäessä määrätyn kokoinen, itsejärjestyvä DNA-rakenne (B–A–B-kompleksi). Kompleksi ohjattiin kullasta valmistettujen nanoelektrodien väliin dielektroforeesin ja tioli-kulta sidoksen avulla. Streptavidiinin avulla osoitettiin, että kompleksi voidaan funktionalisoida muilla molekyyleillä. B–A–B-kompleksin mitattu johtavuus oli lähes olematonta. Kehitetty kompleksi voisi toimia alustana, johon kyettäisiin tarkasti sijoittamaan muita molekyylielektroniikan komponentteja. Toiseksi hyödynnettiin kitosaanin ja DNA:n sähköstaattista vuorovaikutusta valmistettaessa kitosaani-DNA nanopartikkeleita geenien kuljettamiksi. Nanopartikkelit funktionalisoitiin fluoresoivilla leimoilla ja kohdentavilla peptideillä. Soluviljelyanalyysissä nanopartikkelit kulkeutuivat kohdesolujen…
Subjects/Keywords: molekyylityökalu
;
nanobioteknologia
;
avidiini
;
DNA
;
kitosaani
;
biomolecular tool
;
nanobiotechnology
;
avidin
;
chitosan
Record Details
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❌
APA ·
Chicago ·
MLA ·
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CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Leppiniemi, J. (2014). The Development of Novel Biomolecular Tools Based on Avidins, DNA and Chitosan
. (Doctoral Dissertation). Tampere University. Retrieved from https://trepo.tuni.fi/handle/10024/95261
Chicago Manual of Style (16th Edition):
Leppiniemi, Jenni. “The Development of Novel Biomolecular Tools Based on Avidins, DNA and Chitosan
.” 2014. Doctoral Dissertation, Tampere University. Accessed January 15, 2021.
https://trepo.tuni.fi/handle/10024/95261.
MLA Handbook (7th Edition):
Leppiniemi, Jenni. “The Development of Novel Biomolecular Tools Based on Avidins, DNA and Chitosan
.” 2014. Web. 15 Jan 2021.
Vancouver:
Leppiniemi J. The Development of Novel Biomolecular Tools Based on Avidins, DNA and Chitosan
. [Internet] [Doctoral dissertation]. Tampere University; 2014. [cited 2021 Jan 15].
Available from: https://trepo.tuni.fi/handle/10024/95261.
Council of Science Editors:
Leppiniemi J. The Development of Novel Biomolecular Tools Based on Avidins, DNA and Chitosan
. [Doctoral Dissertation]. Tampere University; 2014. Available from: https://trepo.tuni.fi/handle/10024/95261
Colorado School of Mines
13.
Zheng, Wan.
Development of well-defined inorganic and polymeric nanostructures for bio-nanoengineering.
Degree: PhD, Metallurgical and Materials Engineering, 2015, Colorado School of Mines
URL: http://hdl.handle.net/11124/17115
► Bio-nanoengineering, as one of the most fascinating branches of nanoengineering, adapts bio-mimetic, bio-inspired, and bio-hybrid approaches to solve problems in diverse fields of nanoengineering. We…
(more)
▼ Bio-nanoengineering, as one of the most fascinating branches of nanoengineering, adapts bio-mimetic, bio-inspired, and bio-hybrid approaches to solve problems in diverse fields of nanoengineering. We study the bio-nanoengineering approaches on four different materials design projects in this thesis, i.e. nanoparticle assembly, polymeric antibiotic design, development of robust polymeric membranes to support membrane protein(MP) functions, and the development of stimuli-responsive nanocarriers for drug delivery. We identified a bio-inspired strategy that uses highly specific "click" reaction pairs reminiscent to DNA base-pairing or antigen-antibody interactions in biology to directly assemble nanoparticles into well-defined colloidal "molecular" architectures. Inspired by the structural features of bacterial- invading viruses that kill specific bacterial strains with high potency, we developed a bio-mimetic approach to discover a family of novel and highly efficient polymeric antibiotics that show double selectivity. To exploit MPs as biologically-derived high-performance nanomaterials in engineered systems, we created robust and scalable amphiphilic-block-copolymer-based nanomembranes that mimic lipid bilayers to support MP functions, and developed novel polymersome membranes with switchable surface charge states to study the membrane-dependent, MP-mediated transport and recognition performance. Finally, we performed proof-of-concept study on a stimuli-responsive nanocarrier design that integrates biocompatible and biodegradable chitosan with mesoporous silica nanoparticles (MSNs) via a pH-responsive Schiff base bond for the controlled delivery of pharmaceutical payloads.
Advisors/Committee Members: Liang, Hongjun (advisor), Liberatore, Matthew W. (committee member), Wu, Ning (committee member), Richards, Ryan (committee member).
Subjects/Keywords: nanomaterials; polymer chemistry; bioengineering; Nanotechnology; Nanobiotechnology; Nanostructures; Nanoparticles; Polymers; Colloids; Copolymers
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APA ·
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MLA ·
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CSE |
Export
to Zotero / EndNote / Reference
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APA (6th Edition):
Zheng, W. (2015). Development of well-defined inorganic and polymeric nanostructures for bio-nanoengineering. (Doctoral Dissertation). Colorado School of Mines. Retrieved from http://hdl.handle.net/11124/17115
Chicago Manual of Style (16th Edition):
Zheng, Wan. “Development of well-defined inorganic and polymeric nanostructures for bio-nanoengineering.” 2015. Doctoral Dissertation, Colorado School of Mines. Accessed January 15, 2021.
http://hdl.handle.net/11124/17115.
MLA Handbook (7th Edition):
Zheng, Wan. “Development of well-defined inorganic and polymeric nanostructures for bio-nanoengineering.” 2015. Web. 15 Jan 2021.
Vancouver:
Zheng W. Development of well-defined inorganic and polymeric nanostructures for bio-nanoengineering. [Internet] [Doctoral dissertation]. Colorado School of Mines; 2015. [cited 2021 Jan 15].
Available from: http://hdl.handle.net/11124/17115.
Council of Science Editors:
Zheng W. Development of well-defined inorganic and polymeric nanostructures for bio-nanoengineering. [Doctoral Dissertation]. Colorado School of Mines; 2015. Available from: http://hdl.handle.net/11124/17115
RMIT University
14.
Zohora, Nafisa.
Shape controlled biosynthesis of gold nanoprisms using Cinnamomum tamala leaf extract for mercuric ion sensing.
Degree: 2014, RMIT University
URL: http://researchbank.rmit.edu.au/view/rmit:160828
► Biosynthesis of metal nanoparticles using microbes, plants and plant extracts provides a viable alternative green approach for nanoparticle synthesis in comparison to conventional chemical approaches.…
(more)
▼ Biosynthesis of metal nanoparticles using microbes, plants and plant extracts provides a viable alternative green approach for nanoparticle synthesis in comparison to conventional chemical approaches. The use of biosynthesis offers significant advantages over chemical synthesis in context of reduction of energy consumption, generation of non-hazardous by-products and the potential biocompatibility of nanomaterials. The idea behind the current work focuses on understanding the role of the physico-chemical environment in controlling the optical properties of gold nanoparticles synthesised using Cinnamomum tamala leaf extract. This control over the optical property was utilised to show the applicability of gold nanoparticles for mercuric ion sensing. This research therefore provides important information on the role of physical (extraction method, temperature and amount of reducing agent) and chemical (halide ions and surfactant) environment in controlling (i) the rate of reduction of gold ions, (ii) the tunability of optical properties, (iii) shape of nanoparticles, and (iv) size of nanoparticles. The work presented herein is divided into four sections each focussing on different aspects of research. Firstly, the important role of physical parameters in controlling the shape, size and optical property of nanoparticles is addressed. Primarily the method of extraction was assessed to provide experimental evidence for providing reproducible optical tuning of the gold nanoparticles. Further, as temperature is known to influence the rate of reduction of metal ions and reaction kinetics, synthesis of gold nanoparticles at different temperatures using different amount of plant extracts established its critical role in controlling the size and shape of gold nanoparticles. The use of different temperatures allowed high degree of tunability in the surface plasmon resonance with lower temperatures promoting anisotropic growth of nanoparticles. This study, for the first time, also provided mechanistic understanding into the role of functional groups present in plant extract in reducing and stabilising the nanoparticles. Further, the role of externally added chemical agents in controlling the shape, size and optical properties of gold nanoparticles was studied. In particular, the effect of halide ions was addressed and its role in promoting specific facets of gold was studied. More importantly, the role of a surfactant (CTAB) during biosynthesis showed for the first time, branch-like super structures of gold providing clues into its role in assisting hierarchical assemblies. The final aspect of this thesis provided evidence of the practical applicability of biosynthesised gold nanoparticles in mercuric ion sensing. This was achieved by studying the change in the transverse and longitudinal surface plasmon resonance features in response to interaction of biosynthesised gold nanoparticles with mercury ions. This addresses a significant problem as obtaining monodisperse anisotropic nanoparticles is a challenge for mercury ion…
Subjects/Keywords: Fields of Research; Gold Nanoparticles; Nanoprisms; Biosynthesis; Mechanism; Plant extract; Plant Nanotechnology; Nanobiotechnology; Mercury sensing
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APA ·
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Export
to Zotero / EndNote / Reference
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APA (6th Edition):
Zohora, N. (2014). Shape controlled biosynthesis of gold nanoprisms using Cinnamomum tamala leaf extract for mercuric ion sensing. (Thesis). RMIT University. Retrieved from http://researchbank.rmit.edu.au/view/rmit:160828
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Zohora, Nafisa. “Shape controlled biosynthesis of gold nanoprisms using Cinnamomum tamala leaf extract for mercuric ion sensing.” 2014. Thesis, RMIT University. Accessed January 15, 2021.
http://researchbank.rmit.edu.au/view/rmit:160828.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Zohora, Nafisa. “Shape controlled biosynthesis of gold nanoprisms using Cinnamomum tamala leaf extract for mercuric ion sensing.” 2014. Web. 15 Jan 2021.
Vancouver:
Zohora N. Shape controlled biosynthesis of gold nanoprisms using Cinnamomum tamala leaf extract for mercuric ion sensing. [Internet] [Thesis]. RMIT University; 2014. [cited 2021 Jan 15].
Available from: http://researchbank.rmit.edu.au/view/rmit:160828.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Zohora N. Shape controlled biosynthesis of gold nanoprisms using Cinnamomum tamala leaf extract for mercuric ion sensing. [Thesis]. RMIT University; 2014. Available from: http://researchbank.rmit.edu.au/view/rmit:160828
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
RMIT University
15.
Li, V.
Advancing silver nanostructures towards antibacterial applications.
Degree: 2014, RMIT University
URL: http://researchbank.rmit.edu.au/view/rmit:160929
► There is a growing concern on the emergence and re-emergence of drug-resistant pathogens such as multi-resistant bacterial strains. Hence, the development of new antimicrobial compounds…
(more)
▼ There is a growing concern on the emergence and re-emergence of drug-resistant pathogens such as multi-resistant bacterial strains. Hence, the development of new antimicrobial compounds or the modification of those that already exist in order to improve antimicrobial activity is a high priority area of research. Silver has a strong antimicrobial potential, which has been exploited since ancient times. Therefore, the exploration of various Ag-based nanomaterials for antimicrobial applications was pursued. A range of Ag nanomaterials were synthesised, characterised and tested for their antibacterial activity. Different shapes of Ag nanoparticles (spheres, cubes and prisms) were synthesised and tested for their antibacterial activity against model strains of Gram negative bacteria Escherichia coli and Gram positive bacteria Staphylococcus albus . All shapes of Ag nanoparticles exhibited antibacterial activity against both bacterial strains. However, Ag nanocubes exhibited the highest antibacterial activity. Tyrosine reduced spherical Ag nanoparticles were selected as the model Ag nanoparticles for further studies. These nanoparticles were employed as antibiotic carriers for antibacterial activity. Traditional antibiotics (ampicillin, penicillin G and polymyxin B) were utilised as functional conjugates to influence antibacterial capabilities on the surface of Ag nanoparticles. The combination of Ag nanoparticles and antibiotics demonstrated synergistic effects at lower concentrations of silver and revealed physical mode of action against bacteria causing cell wall cleavage and lysis. The control of surface functionalisation and composition of nanoparticles via a green approach was also achieved. In particular, three particular phenolic compounds including tyrosine, curcumin and epigallocatechin gallate (EGCG) were utilised as reducing as well as capping agent to synthesise functional Ag nanoparticles. These phenolic compounds incorporate one or multiple phenolic groups which were instigated as organic surface coronas. Ag nanoparticles containing equimolar and various mole ratios of these phenolic compounds were synthesised. These functionalised Ag nanoparticles were tested for their antibacterial activity and a correlation between surface coronas and composition of nanoparticles was studied. The functionalised Ag nanoparticles of various mole ratios all exhibited significant antibacterial activity with physical damage to bacterial cells. Efforts were made to understand the role of surface functionalisation of Ag nanoparticles in dictating the ability of these nanoparticles to differentially interact with bacterial membranes. This led to the mechanistic insight into the antibacterial performance of Ag nanomaterials. Electrochemical and biological techniques were elucidated to understand the proposed mechanism of Ag nanomaterials’ interaction with Gram negative and Gram positive bacterial cells. The interaction of nanoparticles with bacterial membrane proteins was also studied. Membrane protein studies demonstrated…
Subjects/Keywords: Fields of Research; nanotechnology; nanobiotechnology; silver nanoparticles; antibacterial; E. coli; S. albus
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APA ·
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Manager
APA (6th Edition):
Li, V. (2014). Advancing silver nanostructures towards antibacterial applications. (Thesis). RMIT University. Retrieved from http://researchbank.rmit.edu.au/view/rmit:160929
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Li, V. “Advancing silver nanostructures towards antibacterial applications.” 2014. Thesis, RMIT University. Accessed January 15, 2021.
http://researchbank.rmit.edu.au/view/rmit:160929.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Li, V. “Advancing silver nanostructures towards antibacterial applications.” 2014. Web. 15 Jan 2021.
Vancouver:
Li V. Advancing silver nanostructures towards antibacterial applications. [Internet] [Thesis]. RMIT University; 2014. [cited 2021 Jan 15].
Available from: http://researchbank.rmit.edu.au/view/rmit:160929.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Li V. Advancing silver nanostructures towards antibacterial applications. [Thesis]. RMIT University; 2014. Available from: http://researchbank.rmit.edu.au/view/rmit:160929
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Ryerson University
16.
Keshavarz, Meysam.
Hybrid Silicon-Based Nanomaterials Synthesized Via Femtosecond Laser For Theranostics Applications.
Degree: 2017, Ryerson University
URL: https://digital.library.ryerson.ca/islandora/object/RULA%3A6896
► Biocompatibility and bio-stability are two very important criteria of the materials being used in biology and biological related applications. Among biocompatible materials, Silicon has been…
(more)
▼ Biocompatibility and bio-stability are two very important criteria of the materials being used in biology and biological related applications. Among biocompatible materials, Silicon has been widely used in biomedical applications such as logistic for drug delivery and scaffolds for tissue engineering. Therefore, Silicon is chosen to be further investigated in terms of its potential therapeutic and diagnostic applications. The main objective of this thesis is to explore capabilities
of silicon-based nanomaterials on therapeutic and diagnostic of cancer HeLa and mammalian Fibroblast cell lines. The uniqueness of silicon-based nanomaterials synthesized in this study relies on utilization of femtosecond laser, a versatile yet precise method to generate nanoscale siliconbased materials.
In the initial phase of this study, the need for manufacturing an alternative tool to modulate cells behavior is addressed. During this phase, it is shown that Induced Residual Stress (IRS) onto the Silicon chip via Ultrashort Pulsed Laser (USPL) irradiation is remarkably capable of remotely tuning cellular behavior by which the fate and directionality of seeded cells could be modulated. The second phase is dedicated to synthesis of a novel nanostructure to overcome the limitations of using Silicon-based bio-template for therapeutic applications. Thus far, Nano-scale silicon material has been employed in the form of either nanoparticles (NPs) or 3-D structure for drug delivery and scaffold respectively. Here, a self-assembled Silica Nano-web (SNW) that concurrently exhibits the therapeutic and proliferative attributions of NPs and 3-D structure is introduced. This SNW also demonstrates selective functionality by which mammalian cells and cancer cells are treated differently.
The third phase of this study focuses on diagnostic applications of the silicon-based materials. In this phase, a label-free multiplex photoluminescent silicon nano-probe (PLSN-probe) as a potential substitute for quantum dots (QDs) in bioimaging is synthesized. An inherently nonphotoluminescent silicon substrate is altered to create the PLSN-probe, which overcomes the major drawbacks of presently available QDs. The PLSN-probe not only demonstrates unique optical properties that can be utilized for bioimaging but also exhibits cell-selective uptake that allows the differentiation and diagnosis of HeLa and fibroblast cells. Last but not least, the final study is a unique approach of using polygonal Silicon quantum-probe (polygonal Si-QP) that utilizes a surface-enhanced Raman scattering (SERS) for diagnosis and differentiating of cancerous HeLa and fibroblast cells. It is demonstrated that the polygonal SiQPs could be used for in-situ live cell analysis. Live Raman sensing has also exhibited promising outcome by which tracing of chemical transition in a cell could lead us to a better understanding of cellular changes.
The obtained results indicate multifunctional feasibility of nano/quantum scale silicon based structures for therapeutic, bioimaging and…
Subjects/Keywords: Nanosilicon; Nanobiotechnology – Research; Cancer cells; Nanomedicine – Research; Nanostructured materials – Testing; Drug delivery systems
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APA (6th Edition):
Keshavarz, M. (2017). Hybrid Silicon-Based Nanomaterials Synthesized Via Femtosecond Laser For Theranostics Applications. (Thesis). Ryerson University. Retrieved from https://digital.library.ryerson.ca/islandora/object/RULA%3A6896
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Keshavarz, Meysam. “Hybrid Silicon-Based Nanomaterials Synthesized Via Femtosecond Laser For Theranostics Applications.” 2017. Thesis, Ryerson University. Accessed January 15, 2021.
https://digital.library.ryerson.ca/islandora/object/RULA%3A6896.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Keshavarz, Meysam. “Hybrid Silicon-Based Nanomaterials Synthesized Via Femtosecond Laser For Theranostics Applications.” 2017. Web. 15 Jan 2021.
Vancouver:
Keshavarz M. Hybrid Silicon-Based Nanomaterials Synthesized Via Femtosecond Laser For Theranostics Applications. [Internet] [Thesis]. Ryerson University; 2017. [cited 2021 Jan 15].
Available from: https://digital.library.ryerson.ca/islandora/object/RULA%3A6896.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Keshavarz M. Hybrid Silicon-Based Nanomaterials Synthesized Via Femtosecond Laser For Theranostics Applications. [Thesis]. Ryerson University; 2017. Available from: https://digital.library.ryerson.ca/islandora/object/RULA%3A6896
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Ryerson University
17.
Cruje, Charmainne.
Enhanced uptake of polyethylene glycol coated gold nanoparticles for improved therapeutics.
Degree: 2015, Ryerson University
URL: https://digital.library.ryerson.ca/islandora/object/RULA%3A3705
► Polyethylene glycol (PEG) has promoted the prospective cancer treatment applications of gold nanoparticles (GNPs). PEG is widely used in providing GNPs with stealth properties, hence…
(more)
▼ Polyethylene glycol (PEG) has promoted the prospective cancer treatment applications of gold nanoparticles (GNPs). PEG is widely used in providing GNPs with stealth properties, hence prolonging blood circulation times. GNPs coated with PEG (PEG-GNPs) take advantage of the enhanced permeability and retention effect in tumor environments, making them suitable for targeted treatment. The cellular uptake of PEG-GNPs is significantly lower than uncoated GNPs in vitro. PEG minimizes PEG-GNP interaction with ligands that mediate cancer cell uptake, causing reduced GNP uptake in comparison to uncoated GNP. As intracellular localization of GNPs maximizes its therapeutic enhancement, there is a need to improve the uptake of PEG-GNPs. To improve cell entry, receptor mediated endocytosis peptides were conjugated with PEG-GNPs of varying core sizes. Spherical GNPs of diameters 14, 50 and 70 nm with a PEG chain length of 2 kDa were used to determine a preferred core size for uptake in vitro in HeLa and MDA-MB-231 cells. A preliminary study using surface-modified GNPs as a radiosensitizer to a megavoltage clinical photon beam was done to assess its therapeutic application.
Advisors/Committee Members: Chithrani, Devika (Thesis advisor), Ryerson University (Degree grantor).
Subjects/Keywords: Polyethylene glycol – Therapeutic use; Nanoparticles – Therapeutic use; Cancer – Treatment; Polyethylene glycol – Biotechnology; Nanobiotechnology; Gold
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APA (6th Edition):
Cruje, C. (2015). Enhanced uptake of polyethylene glycol coated gold nanoparticles for improved therapeutics. (Thesis). Ryerson University. Retrieved from https://digital.library.ryerson.ca/islandora/object/RULA%3A3705
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Cruje, Charmainne. “Enhanced uptake of polyethylene glycol coated gold nanoparticles for improved therapeutics.” 2015. Thesis, Ryerson University. Accessed January 15, 2021.
https://digital.library.ryerson.ca/islandora/object/RULA%3A3705.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Cruje, Charmainne. “Enhanced uptake of polyethylene glycol coated gold nanoparticles for improved therapeutics.” 2015. Web. 15 Jan 2021.
Vancouver:
Cruje C. Enhanced uptake of polyethylene glycol coated gold nanoparticles for improved therapeutics. [Internet] [Thesis]. Ryerson University; 2015. [cited 2021 Jan 15].
Available from: https://digital.library.ryerson.ca/islandora/object/RULA%3A3705.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Cruje C. Enhanced uptake of polyethylene glycol coated gold nanoparticles for improved therapeutics. [Thesis]. Ryerson University; 2015. Available from: https://digital.library.ryerson.ca/islandora/object/RULA%3A3705
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Universidade de Brasília
18.
Júlia Poubel Coelho.
Avaliação da biocompatibilidade de magnetolipossomas à base de nanopartículas de maghemita.
Degree: 2008, Universidade de Brasília
URL: http://bdtd.bce.unb.br/tedesimplificado/tde_busca/arquivo.php?codArquivo=3355
► Nanobiotechnology represents an emerging and promising research field. Nanostructured materials, such as magnetic fluids and magnetoliposomes (MLs), have been proposed for drug-delivery systems and thermal-based…
(more)
▼ Nanobiotechnology represents an emerging and promising research field. Nanostructured materials, such as magnetic fluids and magnetoliposomes (MLs), have been proposed for drug-delivery systems and thermal-based cancer therapy amongst other several applications in biomedicine. In particular, MLs are physiologically stable structures, consisting of magnetic nanoparticles (MNPs) wrapped by a phospholipid bilayer. A ML sample containing citrate-coated maghemite nanoparticles encapsulated in liposomes (called ML-Magh) was synthesized for biomedical applications purposes. Polyethylene glycol (PEG) was grafted onto the liposome bilayer. The aim of this work was to investigate the biological behaviour of ML-Magh (1,0 × 10 14 particle/ mL), by evaluating their biocompatibility/ toxicity through in vitro and in vivo tests. The particle size and surface charge potential of MLs were determined. They presented a mean particle size of 124 nm and a negative surface charge, as determined by measuring the zeta potential (-24,4 mV). To perform the in vitro test, human submandibular duct cells (HSG) and mesangial cells (CM) were cultivated during 24 hours in the presence of the ML-Magh sample at two different doses (50 L and 100 L). Analysis by light microscopy showed that ML-Magh does not induce morphological alterations on both cellular types. For the in vivo tests, 100 L of the ML-Magh sample were endovenously or intraperitoneally administrated to female Swiss mice (n=5-6). Control animals (n=3) were treated with phosphate buffered saline (PBS). ML-Magh sample effects were investigated from 30 minutes until 30 days after the administration. The viability of peritoneal cells was not affected by the ML-Magh treatment, as investigated by two different methods: nigrosin dye exclusion and propidium iodide exclusion. The leukocytes cytometry indicated that ML-Magh has no pro-inflammatory action. The eosinophil population presented an increase one hour after ML-Magh administration, suggesting a slight allergic process. Absence of genotoxicity and cytotoxicity was confirmed by micronucleus test and polychromatic erythrocyte percentage, respectively. The histopathology analysis performed in three tissues showed few particle clusters in the lungs and spleen. Clusters were not observed in the liver. No morphological alterations were found in these tissues during all the experimental time. The rare inflammatory infiltration observed in the liver and lungs of ML- Magh-treated mice was also observed in control animals. Biochemical blood tests showed irrelevant alterations. Concentrations of alanine aminotransferase (ALT), alkaline phosphatase (ALP), urea, and creatinine were constant almost all over the experiment, suggesting no hepatic or splenic injury. The levels of serum iron were not affected by ML-Magh treatment. Nevertheless, serum iron levels are dose-dependent. Data suggest that the investigated sample is biocompatible and it has potential to be used in biomedical applications, especially as agents for anticancer therapy through…
Advisors/Committee Members: Zulmira Guerrero Marques Lacava, Marcio José Poças Fonseca, Maria Helena de Araujo Guedes.
Subjects/Keywords: Nanotoxicology; Nanobiotecnologia; Nanotoxicologia; Magnetolipossomas; Nanopartículas magnéticas; Biocompatibilidade; Nanobiotechnology; Magnetic nanoparticles; Magnetoliposomes; Biocompatibility; BIOLOGIA MOLECULAR
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APA (6th Edition):
Coelho, J. P. (2008). Avaliação da biocompatibilidade de magnetolipossomas à base de nanopartículas de maghemita. (Thesis). Universidade de Brasília. Retrieved from http://bdtd.bce.unb.br/tedesimplificado/tde_busca/arquivo.php?codArquivo=3355
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Coelho, Júlia Poubel. “Avaliação da biocompatibilidade de magnetolipossomas à base de nanopartículas de maghemita.” 2008. Thesis, Universidade de Brasília. Accessed January 15, 2021.
http://bdtd.bce.unb.br/tedesimplificado/tde_busca/arquivo.php?codArquivo=3355.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Coelho, Júlia Poubel. “Avaliação da biocompatibilidade de magnetolipossomas à base de nanopartículas de maghemita.” 2008. Web. 15 Jan 2021.
Vancouver:
Coelho JP. Avaliação da biocompatibilidade de magnetolipossomas à base de nanopartículas de maghemita. [Internet] [Thesis]. Universidade de Brasília; 2008. [cited 2021 Jan 15].
Available from: http://bdtd.bce.unb.br/tedesimplificado/tde_busca/arquivo.php?codArquivo=3355.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Coelho JP. Avaliação da biocompatibilidade de magnetolipossomas à base de nanopartículas de maghemita. [Thesis]. Universidade de Brasília; 2008. Available from: http://bdtd.bce.unb.br/tedesimplificado/tde_busca/arquivo.php?codArquivo=3355
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
19.
Ediz, Ege.
Phaseolus vulgaris l.'den gümüş nanopartiküllerin biyosentezi ve antifungal etkinliklerinin incelenmesi: Biosynthesis of silver nanoparticles from Phaseolus vulgaris L. and investigation of their antifungal activities.
Degree: Fen Fakültesi, 2018, University of Ankara
URL: http://hdl.handle.net/20.500.12575/68864
► Bu tez çalışmasında, protein içeriği yüksek bir baklagil olan Phaseolus vulgaris L. (Yunus-90) bitkisinin farklı bölümlerinden fito-nano teknolojik yöntemle hazırlanmış gümüş nanopartiküllerin, hedef bitkide patojenik…
(more)
▼ Bu tez çalışmasında, protein içeriği yüksek bir baklagil olan Phaseolus vulgaris L. (Yunus-90) bitkisinin farklı bölümlerinden fito-nano teknolojik yöntemle hazırlanmış gümüş nanopartiküllerin, hedef bitkide patojenik etki gösterdiği bilinen çeşitli fungus türlerine karşı antifungal etkinliklerinin tespit edilmesi hedeflenmiştir. Araştırmanın ilk bölümünde, çalışma kapsamında kullanılacak bitki örnekleri, steril edilen bitki tohumlarından, perlit ortamında Hoagland besi çözeltisi kullanılarak iklim kabininde yetiştirilmiştir. Takip eden aşamada; bitkinin yaprak, gövde ve kök bölgelerinden fito-nanogümüş partiküllerin elde edilmesi gerçekleştirilmiştir. Sentezi başarılı bir biçimde gerçekleştirilen nanopartiküllerin yapısı uygun spektral analiz yöntemleri (UV-vis, FT-IR, TEM, SEM ve DLS) kullanılarak aydınlatılmıştır. Tez çalışmasının son bölümünde, Colletotrichum sp., Fusarium oxysporum, F. acuminatum, F. tricinctum, F. graminearum, F. incarnatum, F. culvarum, Rhizoctonia solani, Sclerotinia sclerotiorum, Alternaria alternata ve Sclerotium rolfsii fungal patojen türlerinin gelişimlerinin baskılanmasında, hem fito-nano yöntemle hem de standart kimyasal yöntemlerle sentezlenen gümüş nanopartiküllerin etkinlikleri karşılaştırmalı olarak incelenmiştir.
In this thesis, we aimed to synthesize silver nanoparticles by the phyto-nanotechnological method from the different parts of Phaseolus vulgaris L. (Yunus-90), a highly proteinaceous plant legume, and to determine their antifungal activities against various fungi species known to have a pathogenic effect on the target plant organism. In the first part of the study, plant samples to be used during the study were grown from the sterilized plant seeds in a climate cabinet using Hoagland's nutrient solution in the perlite medium. The following step; includes the preparation of phyto-nanoparticles from leaf, stem and root regions of the plant. The structure of the successfully synthesized nanoparticles was elucidated using appropriate spectral analysis methods (UV-vis, FT-IR, TEM, SEM, and DLS). In the last part of the study, the efficacy of silver nanoparticles synthesized by both phyto-nano method and standard chemical methods in the suppression of Colletotrichum sp., Fusarium oxysporum, F. acuminatum, F. tricinctum, F. graminearum, F. incarnatum, F. culvarum, Rhizoctonia solani, Sclerotinia sclerotiorum, Alternaria alternata, and Sclerotium rolfsii's fungal pathogen isolate development has been comparatively investigated.
Advisors/Committee Members: Aras, Sümer (advisor).
Subjects/Keywords: Fito nanobiyoteknoloji; Yeşil sentez; Gümüş nanopartikül; Phyto nanobiotechnology; Green synthesis; Phaseolus vulgaris
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APA ·
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Export
to Zotero / EndNote / Reference
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APA (6th Edition):
Ediz, E. (2018). Phaseolus vulgaris l.'den gümüş nanopartiküllerin biyosentezi ve antifungal etkinliklerinin incelenmesi: Biosynthesis of silver nanoparticles from Phaseolus vulgaris L. and investigation of their antifungal activities. (Masters Thesis). University of Ankara. Retrieved from http://hdl.handle.net/20.500.12575/68864
Chicago Manual of Style (16th Edition):
Ediz, Ege. “Phaseolus vulgaris l.'den gümüş nanopartiküllerin biyosentezi ve antifungal etkinliklerinin incelenmesi: Biosynthesis of silver nanoparticles from Phaseolus vulgaris L. and investigation of their antifungal activities.” 2018. Masters Thesis, University of Ankara. Accessed January 15, 2021.
http://hdl.handle.net/20.500.12575/68864.
MLA Handbook (7th Edition):
Ediz, Ege. “Phaseolus vulgaris l.'den gümüş nanopartiküllerin biyosentezi ve antifungal etkinliklerinin incelenmesi: Biosynthesis of silver nanoparticles from Phaseolus vulgaris L. and investigation of their antifungal activities.” 2018. Web. 15 Jan 2021.
Vancouver:
Ediz E. Phaseolus vulgaris l.'den gümüş nanopartiküllerin biyosentezi ve antifungal etkinliklerinin incelenmesi: Biosynthesis of silver nanoparticles from Phaseolus vulgaris L. and investigation of their antifungal activities. [Internet] [Masters thesis]. University of Ankara; 2018. [cited 2021 Jan 15].
Available from: http://hdl.handle.net/20.500.12575/68864.
Council of Science Editors:
Ediz E. Phaseolus vulgaris l.'den gümüş nanopartiküllerin biyosentezi ve antifungal etkinliklerinin incelenmesi: Biosynthesis of silver nanoparticles from Phaseolus vulgaris L. and investigation of their antifungal activities. [Masters Thesis]. University of Ankara; 2018. Available from: http://hdl.handle.net/20.500.12575/68864
University of Miami
20.
Vannoy, Charles H.
Behavioral Effects of Functionalized CdSe/ZnS Quantum Dots in Self-Organization and Protein Fibrillation.
Degree: PhD, Chemistry (Arts and Sciences), 2010, University of Miami
URL: https://scholarlyrepository.miami.edu/oa_dissertations/431
► Advances in recent nanoscience technologies have generated a new compilation of biocompatible, fluorescent nanoparticles derived from semiconductor quantum dots (QDs). QDs are extremely small…
(more)
▼ Advances in recent nanoscience technologies have generated a new compilation of biocompatible, fluorescent nanoparticles derived from semiconductor quantum dots (QDs). QDs are extremely small in size and possess very large surface areas, which gives them unique physical properties and applications distinct from those of bulk systems. When exposed to biological fluid, these QDs may become coated with proteins and other biomolecules given their dynamic nature. These protein-QD systems may affect or enhance the changes in protein structure and stability, leading to the destruction of biological function. It is believed that these QDs can act as nucleation centers and subsequently promote protein fibril formation. Protein fibrillation is closely associated with many fatal human diseases, including neurodegenerative diseases and a variety of systemic amyloidoses. This topic of protein-QD interaction brings about many key issues and concerns, especially with respect to the potential risks to human health and the environment. Herein, the behavioral effects of dihydrolipoic acid (DHLA)-capped CdSe/ZnS (core/shell) QDs in hen egg-white lysozyme (HEWL) and human serum albumin (HSA) protein systems were systematically analyzed. This study gives rise to a better understanding of the potentially useful application of these protein-QD systems in
nanobiotechnology and nanomedicine as a bioimaging tool and/or as a reference for controlled biological self-assembly processes.
Advisors/Committee Members: Francisco M. Raymo, Thomas K. Harris, Roger M. Leblanc, George C. Boynton.
Subjects/Keywords: Human Serum Albumin; Protein Fibrils; Hen Egg-white Lysozyme; Nanobiotechnology; Protein-nanoparticle Interactions
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APA (6th Edition):
Vannoy, C. H. (2010). Behavioral Effects of Functionalized CdSe/ZnS Quantum Dots in Self-Organization and Protein Fibrillation. (Doctoral Dissertation). University of Miami. Retrieved from https://scholarlyrepository.miami.edu/oa_dissertations/431
Chicago Manual of Style (16th Edition):
Vannoy, Charles H. “Behavioral Effects of Functionalized CdSe/ZnS Quantum Dots in Self-Organization and Protein Fibrillation.” 2010. Doctoral Dissertation, University of Miami. Accessed January 15, 2021.
https://scholarlyrepository.miami.edu/oa_dissertations/431.
MLA Handbook (7th Edition):
Vannoy, Charles H. “Behavioral Effects of Functionalized CdSe/ZnS Quantum Dots in Self-Organization and Protein Fibrillation.” 2010. Web. 15 Jan 2021.
Vancouver:
Vannoy CH. Behavioral Effects of Functionalized CdSe/ZnS Quantum Dots in Self-Organization and Protein Fibrillation. [Internet] [Doctoral dissertation]. University of Miami; 2010. [cited 2021 Jan 15].
Available from: https://scholarlyrepository.miami.edu/oa_dissertations/431.
Council of Science Editors:
Vannoy CH. Behavioral Effects of Functionalized CdSe/ZnS Quantum Dots in Self-Organization and Protein Fibrillation. [Doctoral Dissertation]. University of Miami; 2010. Available from: https://scholarlyrepository.miami.edu/oa_dissertations/431
University of California – San Francisco
21.
Fox, Cade.
Polymeric Micro- and Nanofabricated Devices for Oral Drug Delivery.
Degree: Pharmaceutical Sciences and Pharmacogenomics, 2016, University of California – San Francisco
URL: http://www.escholarship.org/uc/item/4cq4w4xc
► While oral drug administration is by far the most preferred route, it is accompanied by many barriers that limit drug uptake such as the low…
(more)
▼ While oral drug administration is by far the most preferred route, it is accompanied by many barriers that limit drug uptake such as the low pH of the stomach, metabolic and proteolytic enzymes, and limited permeability of the intestinal epithelium. As a result, many drugs ranging from small molecules to biological therapeutics have limited oral bioavailability, precluding them from oral administration. To address this issue, microfabrication has been applied to create planar, asymmetric devices capable of binding to the lining of the gastrointestinal tract and releasing drug at high concentrations, thereby increasing oral drug uptake. While the efficacy of these devices has been validated in vitro and in vivo, modifying their surfaces with nanoscale features has potential to refine their properties for enhanced drug delivery. This dissertation first presents an approach to fabricate polymeric microdevices coated with nanowires in a rapid, high throughput manner. The nanowires demonstrate rapid drug localization onto the surface of these devices via capillary action and increased adhesion to epithelial tissue, suggesting that this fabrication technique can be used to create devices with enhanced properties for oral drug delivery. Also presented are microdevices sealed with nanostraw membranes. The nanostraw membranes provide sustained drug release by limiting drug efflux from the devices, prevent drug degradation by limiting influx of outside biomolecules, and enhance device bioadhesion by penetrating into the mucus layer of the intestinal lining. Finally, an approach that dramatically increases the capacity and efficiency of drug loading into microdevices over previous methods is presented. A picoliter-volume printer is used to print drug directly into device reservoirs in an automated fashion. The technologies presented here expand the capabilities of microdevices for oral drug delivery by incorporating nanoscale structures that enhance device bioadhesion, tunability of drug release, and drug protection and also provide a more cost-effective and scalable approach to drug loading.
Subjects/Keywords: Pharmaceutical sciences; Nanotechnology; Microdevice; Nanobiotechnology; Nanostraws; Nanowires; Oral Drug Delivery; Picoliter-volume printing
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APA (6th Edition):
Fox, C. (2016). Polymeric Micro- and Nanofabricated Devices for Oral Drug Delivery. (Thesis). University of California – San Francisco. Retrieved from http://www.escholarship.org/uc/item/4cq4w4xc
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Fox, Cade. “Polymeric Micro- and Nanofabricated Devices for Oral Drug Delivery.” 2016. Thesis, University of California – San Francisco. Accessed January 15, 2021.
http://www.escholarship.org/uc/item/4cq4w4xc.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Fox, Cade. “Polymeric Micro- and Nanofabricated Devices for Oral Drug Delivery.” 2016. Web. 15 Jan 2021.
Vancouver:
Fox C. Polymeric Micro- and Nanofabricated Devices for Oral Drug Delivery. [Internet] [Thesis]. University of California – San Francisco; 2016. [cited 2021 Jan 15].
Available from: http://www.escholarship.org/uc/item/4cq4w4xc.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Fox C. Polymeric Micro- and Nanofabricated Devices for Oral Drug Delivery. [Thesis]. University of California – San Francisco; 2016. Available from: http://www.escholarship.org/uc/item/4cq4w4xc
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
22.
RAFI RASHID.
THE BIOPHYSICAL PROPERTIES OF MACROMOLECULAR CROWDERS AND THEIR UPTAKE, DISTRIBUTION AND FATE IN CELLS.
Degree: 2012, National University of Singapore
URL: http://scholarbank.nus.edu.sg/handle/10635/47653
Subjects/Keywords: Crowding; Ficoll; stem cells; diffusion; nanobiotechnology
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APA (6th Edition):
RASHID, R. (2012). THE BIOPHYSICAL PROPERTIES OF MACROMOLECULAR CROWDERS AND THEIR UPTAKE, DISTRIBUTION AND FATE IN CELLS. (Thesis). National University of Singapore. Retrieved from http://scholarbank.nus.edu.sg/handle/10635/47653
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
RASHID, RAFI. “THE BIOPHYSICAL PROPERTIES OF MACROMOLECULAR CROWDERS AND THEIR UPTAKE, DISTRIBUTION AND FATE IN CELLS.” 2012. Thesis, National University of Singapore. Accessed January 15, 2021.
http://scholarbank.nus.edu.sg/handle/10635/47653.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
RASHID, RAFI. “THE BIOPHYSICAL PROPERTIES OF MACROMOLECULAR CROWDERS AND THEIR UPTAKE, DISTRIBUTION AND FATE IN CELLS.” 2012. Web. 15 Jan 2021.
Vancouver:
RASHID R. THE BIOPHYSICAL PROPERTIES OF MACROMOLECULAR CROWDERS AND THEIR UPTAKE, DISTRIBUTION AND FATE IN CELLS. [Internet] [Thesis]. National University of Singapore; 2012. [cited 2021 Jan 15].
Available from: http://scholarbank.nus.edu.sg/handle/10635/47653.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
RASHID R. THE BIOPHYSICAL PROPERTIES OF MACROMOLECULAR CROWDERS AND THEIR UPTAKE, DISTRIBUTION AND FATE IN CELLS. [Thesis]. National University of Singapore; 2012. Available from: http://scholarbank.nus.edu.sg/handle/10635/47653
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Massey University
23.
Stephenson, Adam Wayne Ian.
Supramolecular helical arrangement of porphyrins along DNA : a thesis submitted in the partial fulfilment of the requirements for the degree of Doctor of Philosophy in Chemistry, Massey University, Palmerston North, New Zealand
.
Degree: 2010, Massey University
URL: http://hdl.handle.net/10179/1854
► Porphyrins are useful chromophores and have been used in numerous biological applications including light harvesting, oxygen transport and energy transfer. DNA is a perfect template…
(more)
▼ Porphyrins are useful chromophores and have been used in numerous biological
applications including light harvesting, oxygen transport and energy transfer. DNA is a
perfect template for the controlled assembly of organic chromophores. By combining
DNA and porphyrins in a controlled manner we have developed a novel range of
porphyrin-DNA supramolecular constructs for future applications in
nanobiotechnology.
A number of β-pyrrolic functionalised porphyrin precursors were synthesised to be used
as building blocks in the construction of both covalently and non-covalently modified
DNAs. Using these porphyrins we have created several lipophilic porphyrin-DNA
complexes through non-covalent attachment methods. Using a CuI catalysed azide
alkyne cycloaddition (CuAAC) reaction of azido functionalised porphyrins we have
developed a versatile approach for the covalent, site specific internal porphyrin insertion
into oligonucleotides in a post-synthetic manner. We have investigated a number of
duplex structures where porphyrins were located in the major or minor grooves of the
duplex. Additionally, porphyrins were studied as intercalating moieties when they were
inserted as a bulge in the middle of the duplexes or parallel triplexes. Additionally,
when porphyrins were placed in both strands of the duplex they formed a zipper type
structure in the minor groove. This resulted in a significant increase in the duplex
thermal stability due to the formation of porphyrin H-aggregates. UV-Vis and CD
spectroscopy as well as molecular modelling were used to help understand the
interactions between porphyrins in the duplex.
These findings lay the foundation for the future design of artificial DNA-chromophore
supramolecular architectures and for their applications in material science and
nanotechnology.
Subjects/Keywords: Chromophores;
Nanobiotechnology;
DNA modification;
Porphyrin interactions
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APA (6th Edition):
Stephenson, A. W. I. (2010). Supramolecular helical arrangement of porphyrins along DNA : a thesis submitted in the partial fulfilment of the requirements for the degree of Doctor of Philosophy in Chemistry, Massey University, Palmerston North, New Zealand
. (Thesis). Massey University. Retrieved from http://hdl.handle.net/10179/1854
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Stephenson, Adam Wayne Ian. “Supramolecular helical arrangement of porphyrins along DNA : a thesis submitted in the partial fulfilment of the requirements for the degree of Doctor of Philosophy in Chemistry, Massey University, Palmerston North, New Zealand
.” 2010. Thesis, Massey University. Accessed January 15, 2021.
http://hdl.handle.net/10179/1854.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Stephenson, Adam Wayne Ian. “Supramolecular helical arrangement of porphyrins along DNA : a thesis submitted in the partial fulfilment of the requirements for the degree of Doctor of Philosophy in Chemistry, Massey University, Palmerston North, New Zealand
.” 2010. Web. 15 Jan 2021.
Vancouver:
Stephenson AWI. Supramolecular helical arrangement of porphyrins along DNA : a thesis submitted in the partial fulfilment of the requirements for the degree of Doctor of Philosophy in Chemistry, Massey University, Palmerston North, New Zealand
. [Internet] [Thesis]. Massey University; 2010. [cited 2021 Jan 15].
Available from: http://hdl.handle.net/10179/1854.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Stephenson AWI. Supramolecular helical arrangement of porphyrins along DNA : a thesis submitted in the partial fulfilment of the requirements for the degree of Doctor of Philosophy in Chemistry, Massey University, Palmerston North, New Zealand
. [Thesis]. Massey University; 2010. Available from: http://hdl.handle.net/10179/1854
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Cornell University
24.
Lott, Gus III.
Hybridizing Cellular and Behavioral Neurobiology with Modern Engineering Tools: Microelectronics, Microfabricated Devices, and Software Solutions for Physiology.
Degree: 2007, Cornell University
URL: http://hdl.handle.net/1813/7530
► Trained as an electrical engineer and physicist, I have learned the language of the modern neurobiologist and analyzed the state of experimental metrology (measurement tools)…
(more)
▼ Trained as an electrical engineer and physicist, I have learned the language of the modern neurobiologist and analyzed the state of experimental metrology (measurement tools) and laboratory based undergraduate education in the field (Neurobiology & Behavior). As a result of this examination, I develop solutions that offer a new kind of resolution and accessibility to physiological preparations from the sub-cellular to organismal level. My goal is to create an instrumentation toolset that trivializes the solutions to many of the current questions in neurophysiology and enable the experimenter to access higher resolution and new kinds of data about a system. My goal is to allow for new kinds of questions to be asked and answered. This represents the essence of mission of biophysics: Bringing the tools and methods of modern physics to revolutionize studies of biological systems.
I present three distinct projects which illustrate my findings. These projects bring the majority of modern electrical engineering tools and methods to focus on physiological questions in neural systems. The first project illustrates how a time critical microcontroller driven data acquisition and instrumentation system revolutionizes behavioral analysis in model organisms. The second projects brings the tools of the worlds foremost biophysical nanotechnology center (Cornell?s Nanobiotechnology Center, NBTC) to the design of a new kind of polymer substrate microelectrode structure capable of implantation and extracellular recording from sub-millimeter processes in intact animals. The third project describes a software data acquisition and analysis program capable of acquiring data and analyzing events in a variety of useful ways.
These projects should be viewed as responses to questions about the method, itself, of making observations and analysis in neurophysiological research and education environments. These projects describe tools that are currently being applied at a variety of institutions including several affiliates of Cornell to produce dramatic results. It is my goal to bring the experimental mind of yesterday?s behavioral and cellular neurobiologist into the realm of today?s biophysical engineering tools.
Subjects/Keywords: nanobiotechnology; instrumentation; ormia; crayfish; software; education; neurobiology; engineering
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APA (6th Edition):
Lott, G. I. (2007). Hybridizing Cellular and Behavioral Neurobiology with Modern Engineering Tools: Microelectronics, Microfabricated Devices, and Software Solutions for Physiology. (Thesis). Cornell University. Retrieved from http://hdl.handle.net/1813/7530
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Lott, Gus III. “Hybridizing Cellular and Behavioral Neurobiology with Modern Engineering Tools: Microelectronics, Microfabricated Devices, and Software Solutions for Physiology.” 2007. Thesis, Cornell University. Accessed January 15, 2021.
http://hdl.handle.net/1813/7530.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Lott, Gus III. “Hybridizing Cellular and Behavioral Neurobiology with Modern Engineering Tools: Microelectronics, Microfabricated Devices, and Software Solutions for Physiology.” 2007. Web. 15 Jan 2021.
Vancouver:
Lott GI. Hybridizing Cellular and Behavioral Neurobiology with Modern Engineering Tools: Microelectronics, Microfabricated Devices, and Software Solutions for Physiology. [Internet] [Thesis]. Cornell University; 2007. [cited 2021 Jan 15].
Available from: http://hdl.handle.net/1813/7530.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Lott GI. Hybridizing Cellular and Behavioral Neurobiology with Modern Engineering Tools: Microelectronics, Microfabricated Devices, and Software Solutions for Physiology. [Thesis]. Cornell University; 2007. Available from: http://hdl.handle.net/1813/7530
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
University of Washington
25.
Yucesoy, Deniz Tanil.
Genetically Engineered Solid Binding Peptides (GEPI) for Surface Biofunctionalization Applications: Immobilization of Enzymes and Antimicrobial Peptides on Solids.
Degree: 2015, University of Washington
URL: http://hdl.handle.net/1773/27521
► Biological activation and functionalization of solid material interfaces by functional integration of biomolecules is emerging as one of the most dynamic fields of research, impacting…
(more)
▼ Biological activation and functionalization of solid material interfaces by functional integration of biomolecules is emerging as one of the most dynamic fields of research, impacting a diverse array of applications. Recent advances in translating the biomolecular mechanisms into the hybrid materials and systems design promise novel methodologies that may transform some of our engineering approaches. One of the key issues on design of such systems is the integration of bioactive molecules at the material interfaces without compromising their spatial distribution, surface organization and orientation-dependent bioactivity within a desired proximity. Here, we propose to demonstrate the effective utilization of specific solid binding peptides as anchoring molecules that can be further functionalized to create chimeric peptides. These chimeric molecules are engineered to have built-in solid binding surface binding property in addition to displayed biological functionality as shown by two different case studies. In case study I, we take the initial steps toward designing multifunctional, enzyme-based platforms by genetically integrating the engineered solid binding peptide tags for tethering redox enzymes onto electrode surfaces. Specifically, utilizing the gold binding peptide (AuBP2) as a molecular erector, we engineered a fusion protein that genetically conjugates to the formate dehydrogenase (FDH) enzyme. Following the binding kinetics and catalytic activity analysis of fusion protein, we created a circuit-based biosensor system and demonstrated the effectiveness of the fusion FDH enzyme electrode over multiple cycles by addition of formate as the substrate. In case study IIA and IIB, the capability of GEPI's on biomolecular surface functionalization was demonstrated. Here, titanium alloy and zirconium implant surfaces were coated with implant binding peptides which were conjugated to another peptide domain which was engineered with antimicrobial property, resulting a chimeric peptide compromising both solid binding (GEPI's) and antimicrobial (AMP) properties. The efficiency of chimeric/bifunctional peptides both in solution and on titanium surface was evaluated in vitro against common oral and orthopedic infectious organisms, S. mutans and S. epidermidis, respectively and a control organism E. coli. Our findings demonstrate the successful utilization of solid binding peptides as anchoring molecules to design engineered peptide-mediated self-integrated electrode systems and medical devices. The molecular recognition based self-organization of solid binding peptides can be extended to develop a wide range of application where they can be part of biosensing, energy harvesting, biomedical platforms build upon their utilization as biological building blocks combining different combinations of solid materials to large repertoire of biomolecules.
Advisors/Committee Members: Tamerler, Candan (advisor).
Subjects/Keywords: Antimicrobial coating; Biomaterials; Biomimetics; Bio-nano interfaces; Biosensors; Nanobiotechnology; Materials Science; Molecular biology; Nanotechnology; materials science and engineering
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APA (6th Edition):
Yucesoy, D. T. (2015). Genetically Engineered Solid Binding Peptides (GEPI) for Surface Biofunctionalization Applications: Immobilization of Enzymes and Antimicrobial Peptides on Solids. (Thesis). University of Washington. Retrieved from http://hdl.handle.net/1773/27521
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Yucesoy, Deniz Tanil. “Genetically Engineered Solid Binding Peptides (GEPI) for Surface Biofunctionalization Applications: Immobilization of Enzymes and Antimicrobial Peptides on Solids.” 2015. Thesis, University of Washington. Accessed January 15, 2021.
http://hdl.handle.net/1773/27521.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Yucesoy, Deniz Tanil. “Genetically Engineered Solid Binding Peptides (GEPI) for Surface Biofunctionalization Applications: Immobilization of Enzymes and Antimicrobial Peptides on Solids.” 2015. Web. 15 Jan 2021.
Vancouver:
Yucesoy DT. Genetically Engineered Solid Binding Peptides (GEPI) for Surface Biofunctionalization Applications: Immobilization of Enzymes and Antimicrobial Peptides on Solids. [Internet] [Thesis]. University of Washington; 2015. [cited 2021 Jan 15].
Available from: http://hdl.handle.net/1773/27521.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Yucesoy DT. Genetically Engineered Solid Binding Peptides (GEPI) for Surface Biofunctionalization Applications: Immobilization of Enzymes and Antimicrobial Peptides on Solids. [Thesis]. University of Washington; 2015. Available from: http://hdl.handle.net/1773/27521
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
26.
ZHANG ZHIPING.
Nanoparticles of biodegradable polymers for cancer chemotherapy.
Degree: 2007, National University of Singapore
URL: http://scholarbank.nus.edu.sg/handle/10635/49059
Subjects/Keywords: Cancer nanotechnology; Chemotherapeutic engineering; Nanobiotechnology; Nanomedicine; Paclitaxel; Taxol®.
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APA ·
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APA (6th Edition):
ZHIPING, Z. (2007). Nanoparticles of biodegradable polymers for cancer chemotherapy. (Thesis). National University of Singapore. Retrieved from http://scholarbank.nus.edu.sg/handle/10635/49059
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
ZHIPING, ZHANG. “Nanoparticles of biodegradable polymers for cancer chemotherapy.” 2007. Thesis, National University of Singapore. Accessed January 15, 2021.
http://scholarbank.nus.edu.sg/handle/10635/49059.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
ZHIPING, ZHANG. “Nanoparticles of biodegradable polymers for cancer chemotherapy.” 2007. Web. 15 Jan 2021.
Vancouver:
ZHIPING Z. Nanoparticles of biodegradable polymers for cancer chemotherapy. [Internet] [Thesis]. National University of Singapore; 2007. [cited 2021 Jan 15].
Available from: http://scholarbank.nus.edu.sg/handle/10635/49059.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
ZHIPING Z. Nanoparticles of biodegradable polymers for cancer chemotherapy. [Thesis]. National University of Singapore; 2007. Available from: http://scholarbank.nus.edu.sg/handle/10635/49059
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Macquarie University
27.
Wan Razali, Wan Aizuddin.
A nanoruby-based photoluminescent probe towards bioimaging applications.
Degree: 2016, Macquarie University
URL: http://hdl.handle.net/1959.14/1252990
► Thesis by publication.
Bibliography:
Chapter 1. General introduction and outline – Chapter 2. Photoluminescent nanoparticles for bioimaging – Chapter 3. Ball-milling method and characterisation of…
(more)
▼ Thesis by publication.
Bibliography:
Chapter 1. General introduction and outline – Chapter 2. Photoluminescent nanoparticles for bioimaging – Chapter 3. Ball-milling method and characterisation of nanocrystals – Chapter 4. Large-scale production and characterisation of biocompatible colloidal nanoalumina – Chapter 5. Mass production of nanorubies photoluminescent probe for bioimaging – Chapter 6. Wide-field time-gated photoluminescence microscopy for fast ultrahigh-sensitivity imaging of photoluminescent probes – Chapter 7. Summary and future scope.
In the past decades, fluorescent organic dyes have been used extensively as molecular probes in bioimaging. However, their irreversible transition to the dark-state, termed photobleaching, photoinduced cytotoxicity, and biodegradibility limit their application scope. Photoluminescent nanomaterials provide a lucrative alternative to the fluorescent organic dyes due to their virtually unlimited photostability, inert core, controllable surface chemistry, biocompatibility, etc. In this study, I report on the development of a new-generation molecular probe based on a photoluminescent nanomaterial, termed as “nanorubies”, which represents ruby nanocrystals of the composition alpha-alumina (α-Al₂O₃) doped with chromium (Cr³⁺). In addition to the photoluminescent nanomaterial merits, nanoruby emission takes place in a remarkably narrow spectral band in the far-red spectral range (692 nm), and characterised by long photoluminescent lifetime (3.7 ms).
The first reported advance of this study addresses development of a top-down, large-scale production of photoluminescent nanomaterials. Top-down methods take advantage of the existing variety of bulk and thin-film solid-state materials for improved prediction and control of the resultant nanomaterial properties. The power of this approach is demonstrated by using high-energy ball milling (HEBM) of alumina (Al₂O₃) and nanoruby. Nanoalumina and nanoruby particles with a mean size less 100 nm in their most stable α-crystallographic phase were produced in gram quantities suitable for biological and biomedical applications. Nanomaterial contamination from zirconia balls used in HEBM was reduced from 19% to 2% using a selective acid etching procedure.
The biocompatibility of the milled nanomaterial was demonstrated by forming stable colloids in water and physiological buffers corroborated by the corresponding measured zeta potentials of +40 mV and -40 mV and characterised by in vitro cytotoxicity assays. The feasibility of anchoring a host of functional groups and biomolecules to milled nanoalumina and nanoruby surfaces was demonstrated by functionalisation of their surface which resulted in decoration of the nanoparticle surface with amino, carboxyl and polyethylene glycol groups suitable for further conjugation with functional biomolecules.
Photoluminescence probes in combination with fast and sensitive imaging systems are much in demand. I describe a wide-field, time-gated photoluminescence microscopy system…
Advisors/Committee Members: Macquarie University. Department of Physics and Astronomy.
Subjects/Keywords: Luminescent probes; Nanoparticles – Optical properties; Photoluminescence; Nanobiotechnology; Imaging systems in medicine; nanoruby; photoluminescent; probe; high-energy; ball mining; bioimaging
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APA ·
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APA (6th Edition):
Wan Razali, W. A. (2016). A nanoruby-based photoluminescent probe towards bioimaging applications. (Doctoral Dissertation). Macquarie University. Retrieved from http://hdl.handle.net/1959.14/1252990
Chicago Manual of Style (16th Edition):
Wan Razali, Wan Aizuddin. “A nanoruby-based photoluminescent probe towards bioimaging applications.” 2016. Doctoral Dissertation, Macquarie University. Accessed January 15, 2021.
http://hdl.handle.net/1959.14/1252990.
MLA Handbook (7th Edition):
Wan Razali, Wan Aizuddin. “A nanoruby-based photoluminescent probe towards bioimaging applications.” 2016. Web. 15 Jan 2021.
Vancouver:
Wan Razali WA. A nanoruby-based photoluminescent probe towards bioimaging applications. [Internet] [Doctoral dissertation]. Macquarie University; 2016. [cited 2021 Jan 15].
Available from: http://hdl.handle.net/1959.14/1252990.
Council of Science Editors:
Wan Razali WA. A nanoruby-based photoluminescent probe towards bioimaging applications. [Doctoral Dissertation]. Macquarie University; 2016. Available from: http://hdl.handle.net/1959.14/1252990
Rutgers University
28.
Lewis, Daniel Raiken, 1984-.
Amphiphilic macromolecule therapeutics to manage cardiovascular disease.
Degree: Chemical and Biochemical Engineering, 2014, Rutgers University
URL: https://rucore.libraries.rutgers.edu/rutgers-lib/42457/
Subjects/Keywords: Cardiovascular system – Diseases – Treatment; Atherosclerosis – Treatment; Macromolecules; Nanobiotechnology
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APA (6th Edition):
Lewis, Daniel Raiken, 1. (2014). Amphiphilic macromolecule therapeutics to manage cardiovascular disease. (Thesis). Rutgers University. Retrieved from https://rucore.libraries.rutgers.edu/rutgers-lib/42457/
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Lewis, Daniel Raiken, 1984-. “Amphiphilic macromolecule therapeutics to manage cardiovascular disease.” 2014. Thesis, Rutgers University. Accessed January 15, 2021.
https://rucore.libraries.rutgers.edu/rutgers-lib/42457/.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Lewis, Daniel Raiken, 1984-. “Amphiphilic macromolecule therapeutics to manage cardiovascular disease.” 2014. Web. 15 Jan 2021.
Vancouver:
Lewis, Daniel Raiken 1. Amphiphilic macromolecule therapeutics to manage cardiovascular disease. [Internet] [Thesis]. Rutgers University; 2014. [cited 2021 Jan 15].
Available from: https://rucore.libraries.rutgers.edu/rutgers-lib/42457/.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Lewis, Daniel Raiken 1. Amphiphilic macromolecule therapeutics to manage cardiovascular disease. [Thesis]. Rutgers University; 2014. Available from: https://rucore.libraries.rutgers.edu/rutgers-lib/42457/
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
29.
Bedford, Erin.
Gold surface nanostructuring for separation and sensing of biomolecules : Nanostructuration des surfaces d'or pour la séparation et la détection de biomolécules.
Degree: Docteur es, Physique et Chimie des Matériaux, 2016, Université Pierre et Marie Curie – Paris VI
URL: http://www.theses.fr/2016PA066527
► La détection de molécules biologiques dans les environnements physiologiques est essentielle aux soins de santé et la surveillance de l'environnement. Dans ces travaux de thèse,…
(more)
▼ La détection de molécules biologiques dans les environnements physiologiques est essentielle aux soins de santé et la surveillance de l'environnement. Dans ces travaux de thèse, nous étudions et utilisons des surfaces d'or pour la détection de biomolécules, avec l'inclusion de composants nanométriques-spécifiquement, des monocouches auto assemblées (SAMs) d'alcane-thiol et des coquilles d'or nanostructurées-dans l'intention d'améliorer les méthodes de détection biomoléculaire. La fonctionnalisation des surfaces d'or avec des SAMs permet un contrôle de la densité et de l'orientation des biomolécules immobilisées. En utilisant la spectroscopie infrarouge de surface, la spectroscopie de photoelectrons X (XPS) ainsi que la modélisation, utilisant la théorie de la fonctionnelle de la densité (DFT), nous avons trouvé que les SAMs à base de chaînes courtes et de chaînes longues des alcane-thiols ont eu des environnements de l'accroche des atomes de soufre différents. De plus, nous avons trouvé que l'immobilisation et la reconnaissance de protéines varie avec la longueur de la chaîne de SAMs ainsi qu'avec la présence d'un réticulant. Dans la seconde partie des travaux, nous avons synthétisé des coquilles d'or nanostructurées sur des particules magnétiques afin de combiner la séparation magnétique et la détection de biomolécules. Nous avons montré qu'elles pouvaient être utilisés comme substrats pour la spectrométrie Raman exaltée de surface (SERS). Afin d'établir une preuve de concept, nous avons réalisé des tests dans lesquels ces particules ont été utilisées pour détecter l'immobilisation d'oligonucléotides et l'hybridation avec SERS.
Detecting biomolecules in physiological environments is critical to health care and environmental monitoring. In this work, we study and use gold surfaces for biomolecule detection while incorporating nanoscale components—specifically, self-assembled monolayers (SAMs) of alkanethiols and gold nanostructured shells—with the goal of improving biomolecule detection methods. Using SAMs to functionalize gold surfaces can offer control over biomolecule binding density and orientation while still keeping the biomolecules near the sensing surface. Using surface IR spectroscopy, x-ray photoelectron spectroscopy (XPS), and density functional theory (DFT) modeling, we found that SAMs of short-chain and long-chain amine-terminated alkanethiols on gold had different sulphur binding environments. We also found that protein binding and recognition on the two different SAMs varied with SAM chain length and was also influenced by the presence of a cross-linker. In the second part of this work, we synthesized gold nanostructured shells on magnetic particles for combined separation and detection of biomolecules. We demonstrated their use as substrates for surface-enhanced Raman spectroscopy (SERS) As a proof-of-concept, we demonstrated the use of these particles to detect oligonucleotide binding and hybridization with SERS using a Raman-tagged oligonucleotide hairpin probe.
Advisors/Committee Members: Gu, Frank (thesis director), Pradier, Claire-Marie (thesis director), Boujday, Souhir (thesis director).
Subjects/Keywords: Nanobiotechnologie; Nanoparticules; Or; Monocouches auto assemblées; Biocapteurs; Spectrométrie Raman exaltée de surface; Nanobiotechnology; Nanoparticles; Gold; 541.3
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APA ·
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APA (6th Edition):
Bedford, E. (2016). Gold surface nanostructuring for separation and sensing of biomolecules : Nanostructuration des surfaces d'or pour la séparation et la détection de biomolécules. (Doctoral Dissertation). Université Pierre et Marie Curie – Paris VI. Retrieved from http://www.theses.fr/2016PA066527
Chicago Manual of Style (16th Edition):
Bedford, Erin. “Gold surface nanostructuring for separation and sensing of biomolecules : Nanostructuration des surfaces d'or pour la séparation et la détection de biomolécules.” 2016. Doctoral Dissertation, Université Pierre et Marie Curie – Paris VI. Accessed January 15, 2021.
http://www.theses.fr/2016PA066527.
MLA Handbook (7th Edition):
Bedford, Erin. “Gold surface nanostructuring for separation and sensing of biomolecules : Nanostructuration des surfaces d'or pour la séparation et la détection de biomolécules.” 2016. Web. 15 Jan 2021.
Vancouver:
Bedford E. Gold surface nanostructuring for separation and sensing of biomolecules : Nanostructuration des surfaces d'or pour la séparation et la détection de biomolécules. [Internet] [Doctoral dissertation]. Université Pierre et Marie Curie – Paris VI; 2016. [cited 2021 Jan 15].
Available from: http://www.theses.fr/2016PA066527.
Council of Science Editors:
Bedford E. Gold surface nanostructuring for separation and sensing of biomolecules : Nanostructuration des surfaces d'or pour la séparation et la détection de biomolécules. [Doctoral Dissertation]. Université Pierre et Marie Curie – Paris VI; 2016. Available from: http://www.theses.fr/2016PA066527
University of Georgia
30.
Chen, Guojun.
Single molecule interaction and conformation study based on atomic force microscopy.
Degree: 2014, University of Georgia
URL: http://hdl.handle.net/10724/27083
► 1. A simple two-step protocol for modification of atomic force microscopy (AFM) tip and substrate by using a "click reaction" has been developed. The modified…
(more)
▼ 1. A simple two-step protocol for modification of atomic force microscopy (AFM) tip and substrate by using a "click reaction" has been developed. The modified tip and substrate would be applied to detect trace amounts of ricin by using
atomic force microscopy. A key feature of the approach is the use of a PEG (polyethylene glycol) derivative functionalized with one thiol and one azide ending group. One end of the PEG was attached to the gold-coated AFM tip by a strong Au-thiol bond.
The azide group hanging at the other end of the immobilized PEG was used for the attachment of an antiricin antibody modified with an alkyne group using a "click reaction". The latter reaction is highly efficient, compatible with the presence of many
functional groups and could proceed under mild reaction conditions. In a separate step, ricin was immobilized on the gold substrate surface that was modified by active esters. For this process, a novel bifunctional reagent was employed containing an
active ester and a thioctic acid moiety. By these modification processes, AFM recognition imaging was used to detect the toxin molecules and the results show fg/mL detection sensitivity, surpassing the existing detection techniques. With measurement of
the unbinding force between the antiricin antibody and ricin, which was statistically determined to be 64.89 +/- 1.67 pN, the single molecular specificity of this sensing technique is realized. 2. The authors report on a study of detecting ricin
molecules immobilized on chemically modified Au (111) surface by chemomechanically mapping the molecular interactions with a chemically modified atomic force microscopy (AFM) tip. AFM images resolved the different fold-up conformations of single ricin
molecule as well as their intramolecule structure of A- and B-chains. AFM force spectroscopy study of the interaction indicates that the unbinding force has a linear relation with the logarithmic force loading rate, which agrees well with calculations
using one-barrier bond dissociation model. 3. Gold self-assembly: Understanding protein adsorption on gold surface bears increasing importance because of surface-induced changes in conformation and bioactivity. Nanofibril structures of protein fibrinogen
(fg) molecules, playing paramount role in blood coagulation, are found self-assembled on a Au(1,1,1) surface without any addition of thrombin, growing in two orientations (longitude and transverse, see figure). 4. The biomedical applications of gold
nanoparticle (GNP) are extraordinarily promising due to its special optical properties. However, before transforming into real clinical test, a systematic understanding of the physiological interactions of GNP becomes imperative. For example, protein-GNP
interactions and their biological consequences are the most fundamental and exigent for the related studies in cell level. In this study, we report on our findings that the interaction of GNP and fibrinogen (fg) could induce blood clot, one important
blood protein, under near-physiological conditions. Firstly, through…
Subjects/Keywords: 1. ricin; atomic force microscopy; polyethylene glycol; 2. atomic force microscopy, bonds (chemical), dissociation, molecular biophysics, molecular configurations, proteins; 3. fibrinogen; gold; nanobiotechnology; nanofibril growth; self-assembly; 4.FIBRINOGEN; GOLD NANOPARTICLE; SELF-ASSEMBLY; NANOTOXITY; NANOBIOTECHNOLOGY
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APA (6th Edition):
Chen, G. (2014). Single molecule interaction and conformation study based on atomic force microscopy. (Thesis). University of Georgia. Retrieved from http://hdl.handle.net/10724/27083
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Chen, Guojun. “Single molecule interaction and conformation study based on atomic force microscopy.” 2014. Thesis, University of Georgia. Accessed January 15, 2021.
http://hdl.handle.net/10724/27083.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Chen, Guojun. “Single molecule interaction and conformation study based on atomic force microscopy.” 2014. Web. 15 Jan 2021.
Vancouver:
Chen G. Single molecule interaction and conformation study based on atomic force microscopy. [Internet] [Thesis]. University of Georgia; 2014. [cited 2021 Jan 15].
Available from: http://hdl.handle.net/10724/27083.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Chen G. Single molecule interaction and conformation study based on atomic force microscopy. [Thesis]. University of Georgia; 2014. Available from: http://hdl.handle.net/10724/27083
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
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