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1.
류, 한석.
How to approach previously diagnosed gastric neoplasms that are not definitely detected in scheduled therapeutic endoscopy.
Degree: 2019, Ajou University
URL: http://repository.ajou.ac.kr/handle/201003/17878 
;
http://dcoll.ajou.ac.kr:9080/dcollection/jsp/common/DcLoOrgPer.jsp?sItemId=000000029013
► BACKGROUND AND AIMS: Endoscopists sometimes encounter unusual cases in which a previously diagnosed gastric neoplasm is not detected in the scheduled therapeutic endoscopy. This study…
(more)
▼ BACKGROUND AND AIMS: Endoscopists sometimes encounter unusual cases in which a previously diagnosed gastric neoplasm is not detected in the scheduled therapeutic endoscopy. This study aimed to analyze the reasons for undetected lesions in scheduled therapeutic endoscopy and to understand how to approach such cases.
METHODS: Between March 2010 and February 2018, that were previously diagnosed as gastric neoplasms in diagnostic endoscopy were not detected in the scheduled therapeutic endoscopy. A total of 32 patients who did not meet any of the exclusion criteria were enrolled. Additionally, 1870 gastric endoscopic submucosal dissection (ESD) cases, performed in the same period, were retrospectively reviewed and compared with the enrolled cases.
RESULTS: Compared with the 1870 conventional ESD cases, the 32 undetected gastric lesion cases showed a significantly smaller tumor size, smaller surface area, and lower sampling ratios in univariate logistic regression analysis (p < 0.002). Multivariate logistic regression analysis showed that tumor size is a significant risk factor for undetected gastric neoplasms (p < 0.009). Of the undetected 32 lesions, 1 (3.1%) was pathologically overestimated and 1 (3.1%) seemed to be due to incorrect localization of the initial tumor. Moreover, 9 of the 32 (28.1%) lesions were partially removed at the initial endoscopic forceps biopsy (EFB) and recurred during surveillance. Among the 9 recurred cases, 2, 4, and 3 neoplasms were found by EFB at the scheduled therapeutic endoscopy, at 3 months, and at 6 months surveillance endoscopy, respectively. The mean time to the detection of gastric neoplasm was 3.0 ± 2.6 (0–6) months. Most of the undetected gastric neoplasm cases (21/32, 65.6%) seemed to be due to complete removal at the initial EFB.
CONCLUSIONS: If a gastric lesion previously detected on endoscopic screening becomes invisible, we recommend careful surveillance endoscopy at regular intervals of at least 6 months, or at least 2 times.
배경 및 목표: 진단내시경에서 확인된 위 신생물이 치료내시경에서 확인되지 않는 경우가 드물게 관찰된다. 치료내시경에서 관찰되지 않는 병변에 대한 원인을 분석하고, 어떻게 추적관찰 할 것인가 생각해보았다.
방법: 2010년 5월부터 2018년 2월까지의 기간동안, 진단 내시경에서 발견되었으나 치료 내시경에서 관찰되지 않은 55개(55명)의 위 신생물이 있었다. 이 중 배제기준을 만족하지 않은 32개의 위신생물(32명)을 연구에 포함시켰다. 또한 동일 기간에 내시경적 점막하 절제술을 시행한 1870개의 위신생물 사례들을 확인하고, 연구대상인 32개(32명)의 위 신생물과 비교하였다.
결과: 내시경적 점막 절제술을 시행한 1870개의 사례와 비교하였을 때, 치료내시경에서 관찰되지 않은 32개의 위 병변들은 단변량 로지스틱 회귀분석에서 통계적으로 유의하게 작은 종양 크기, 종양 표면면적, 샘플 비를 보였다(p < 0.002). 다변량 로지스틱 회귀분석에서는 오직 종양 크기 만이 관찰되지 않는 위병변의 중요한 위험인자임에 대해 통계적으로 의미 있었다(P < 0.009). 치료내시경에서 관찰되지 않은 위 병변 중에서, 1 (1/32, 3.1%)개는 조직학적으로 과 평가 되었고, 1 (1/32, 3.1%)개는 초기 종양의 위치가 잘못 표기된 것으로 보인다. 9 (9/32, 28.1%)개의 병변은 검사 내시경에서 생검 겸자로 부분적으로 제거되어 치료내시경에서 관찰되지 않다가, 남은 부분이 추적관찰에서 재발 한 것으로 보인다. 재발한 9개의 증례 중에, 2개는 치료내시경 생검에서 발견되었고, 4개의 증례는 3개월째 추적내시경 생검에서, 3개의 증례는 6개월째 추적내시경 생검에서 발견되었다. 진단내시경에서서 관찰되지 않은 병변이 추적내시경에서 관찰될 때까지의 평균 시간은 3.0 ± 2.6 (0–6) 개월이었다. 대부분의 관찰되지 않은 위 신생물(21/32, 65.6%)은 초기 진단 내시경에서 생검겸자에 의하여 완전히 제거된 것으로 생각된다.
결론: 진단 내시경에서 관찰된 위 병변이 치료내시경에서 관찰되지 않는 경우, 일정한 간격의 추적내시경을 6개월 이상, 또는 적어도 2회 이상의…
Advisors/Committee Members: 대학원 의학과, 201424564, 류, 한석.
Subjects/Keywords: Stomach Neoplasms; Endoscopy; Neoplasms, residuel
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APA (6th Edition):
류, . (2019). How to approach previously diagnosed gastric neoplasms that are not definitely detected in scheduled therapeutic endoscopy. (Thesis). Ajou University. Retrieved from http://repository.ajou.ac.kr/handle/201003/17878 ; http://dcoll.ajou.ac.kr:9080/dcollection/jsp/common/DcLoOrgPer.jsp?sItemId=000000029013
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
류, 한석. “How to approach previously diagnosed gastric neoplasms that are not definitely detected in scheduled therapeutic endoscopy.” 2019. Thesis, Ajou University. Accessed March 01, 2021.
http://repository.ajou.ac.kr/handle/201003/17878 ; http://dcoll.ajou.ac.kr:9080/dcollection/jsp/common/DcLoOrgPer.jsp?sItemId=000000029013.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
류, 한석. “How to approach previously diagnosed gastric neoplasms that are not definitely detected in scheduled therapeutic endoscopy.” 2019. Web. 01 Mar 2021.
Vancouver:
류 . How to approach previously diagnosed gastric neoplasms that are not definitely detected in scheduled therapeutic endoscopy. [Internet] [Thesis]. Ajou University; 2019. [cited 2021 Mar 01].
Available from: http://repository.ajou.ac.kr/handle/201003/17878 ; http://dcoll.ajou.ac.kr:9080/dcollection/jsp/common/DcLoOrgPer.jsp?sItemId=000000029013.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
류 . How to approach previously diagnosed gastric neoplasms that are not definitely detected in scheduled therapeutic endoscopy. [Thesis]. Ajou University; 2019. Available from: http://repository.ajou.ac.kr/handle/201003/17878 ; http://dcoll.ajou.ac.kr:9080/dcollection/jsp/common/DcLoOrgPer.jsp?sItemId=000000029013
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
University of Zambia
2.
Kamvuma, Kingsley.M.
Value of Procalcitonin and C-Reactive Protein as Early Markers of Bacteraemia Among Patients With Haematological Malignancies Receiving Chemotherapy,Lusaka,Zambia
.
Degree: 2016, University of Zambia
URL: http://dspace.unza.zm:8080/xmlui/handle/123456789/4863
► Introduction: The immune system of patients with haematological malignancies is suppressed during chemotherapy. This renders them vulnerable to frequent infections especially bacterial. Timely diagnosis of…
(more)
▼ Introduction: The immune system of patients with haematological malignancies is suppressed during chemotherapy. This renders them vulnerable to frequent infections especially bacterial. Timely diagnosis of these infections is difficult, because a severe infection may be asymptomatic or manifest only in the form of fever or malaise. There is need to come up with laboratory markers that can detect an infectious process at an early stage.
Aim: The aim of this study was to determine the value of using Procalcitonin (PCT) and C reactive protein (CRP), for early diagnosis of infection in patients with haematological malignancies receiving chemotherapy.
Method: This was a cross sectional study consisting of sixty eight (68) patients with haematological malignancies. Data from each participant including sex, age, clinical and laboratory presentation were collected after obtaining informed consent. Then Specimens were collected for measurement of PCT, CRP and for bacteriological analysis. Patients were divided into two groups; those with a bacterial culture positive and negative result. Procalcitonin and CRP concentrations were compared between groups using t-test and non-parametric statistical tests respectively. The area under ROC curve, sensitivity, specificity, likelihood ratio, and Spearman's correlation coefficient also calculated.
Results: A total of 14 (20.6%) microorganisms were isolated, of which 10 were gram-positive bacteria and 4 were gram-negative bacilli. The mean values of PCT which were 6.1ng/mL in the bacteraemia group and 5.1ng/mL in the non-bacteraemia group, p=0.023 and median CRP values were 24.2 (6.43-48.15) in the bacteraemia and 23.5 (6.03-75.44) in the non-bacteraemia group, p=0.832. The area under curves was 0.52 (95% CI=0.57-0.84) for CRP and 0.70 (95% CI=0.35-0.69) for PCT. PCT value of greater than 5.1ng/mL is diagnostic for infections (sensitivity 71%, specificity 65%) while that of CRP was 21mg/mL with the sensitivity and specificity of 64% and 44% respectively. Elevated levels of PCT as well as fever were significantly associated with bacteraemia.
Conclusion: Procalcitonin measurements can be of value in the early diagnosis of bacteraemia in patients with haematological malignancies. In contrast, the diagnostic sensitivity and specificity
VIII
for CRP was found to be too low in this study to safely rely on it as a marker of bacteraemia in patients with haematological malignancies.
Key words: Procalcitonin (PCT), C reactive protein (CRP), Haematological Malignancies, Bacteraemia Marker
Subjects/Keywords: Hematologic Neoplasms – genetics.
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APA (6th Edition):
Kamvuma, K. M. (2016). Value of Procalcitonin and C-Reactive Protein as Early Markers of Bacteraemia Among Patients With Haematological Malignancies Receiving Chemotherapy,Lusaka,Zambia
. (Thesis). University of Zambia. Retrieved from http://dspace.unza.zm:8080/xmlui/handle/123456789/4863
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Kamvuma, Kingsley M. “Value of Procalcitonin and C-Reactive Protein as Early Markers of Bacteraemia Among Patients With Haematological Malignancies Receiving Chemotherapy,Lusaka,Zambia
.” 2016. Thesis, University of Zambia. Accessed March 01, 2021.
http://dspace.unza.zm:8080/xmlui/handle/123456789/4863.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Kamvuma, Kingsley M. “Value of Procalcitonin and C-Reactive Protein as Early Markers of Bacteraemia Among Patients With Haematological Malignancies Receiving Chemotherapy,Lusaka,Zambia
.” 2016. Web. 01 Mar 2021.
Vancouver:
Kamvuma KM. Value of Procalcitonin and C-Reactive Protein as Early Markers of Bacteraemia Among Patients With Haematological Malignancies Receiving Chemotherapy,Lusaka,Zambia
. [Internet] [Thesis]. University of Zambia; 2016. [cited 2021 Mar 01].
Available from: http://dspace.unza.zm:8080/xmlui/handle/123456789/4863.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Kamvuma KM. Value of Procalcitonin and C-Reactive Protein as Early Markers of Bacteraemia Among Patients With Haematological Malignancies Receiving Chemotherapy,Lusaka,Zambia
. [Thesis]. University of Zambia; 2016. Available from: http://dspace.unza.zm:8080/xmlui/handle/123456789/4863
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
University of Texas Southwestern Medical Center
3.
Mullen, Andrew Robbins.
Reductive Carboxylation Is a Novel Pathway of Glutamine Metabolism That Supports the Growth of Tumor Cells with Metabolic Defects.
Degree: 2013, University of Texas Southwestern Medical Center
URL: http://hdl.handle.net/2152.5/2726
► In growing cancer cells, oxidative metabolism of glucose and glutamine in the mitochondria provide precursors needed for de novo synthesis of proteins, nucleic acids and…
(more)
▼ In growing cancer cells, oxidative metabolism of glucose and glutamine in the mitochondria provide precursors needed for de novo synthesis of proteins, nucleic acids and lipids. Yet, a subset tumors harbor genetic mutations in the electron transport chain or tricarboxylic acid cycle that disable normal oxidative mitochondrial function. Importantly, it has been unknown how these cells generate the biosynthetic precursors required for growth. To address this, I used models of mitochondrial dysfunction in isogenic cancer cell lines and studied their metabolism using a combination of Gas Chromatography- Mass Spectrometry and Nuclear Magnetic Resonance spectroscopy. In all cases, mitochondrial dysfunction stimulated a novel pathway of glutamine metabolism, characterized by reversal of the canonical tricarboxylic acid cycle, termed reductive carboxylation; providing a plausible mechanism for how cancer cells with mitochondrial defects generate biosynthetic precursors required for growth. To gain mechanistic insight into how this unusual pathway was regulated I carried out a targeted metabolomics analysis in our isogenic tumor cell models. This led to the striking discovery that cells engaged in the reductive carboxylation pathway also operate an additional metabolic pathway that, at first glance, would appear to be superfluous and inefficient. Functional characterization of this second pathway revealed, however, that its activity was necessary for the optimal function of the reductive carboxylation pathway. In summary, this work has given us insights into how cancer cells are able to grow in the context of defective mitochondria. Additionally, this has exposed a potential Achilles ’ heel that might be used to selectively kill tumors which rely on this pathway for growth.
Advisors/Committee Members: Burgess, Shawn C., Abrams, John M., Pearson, Gray W., DeBerardinis, Ralph J..
Subjects/Keywords: Glutamine; Mitochondria; Neoplasms
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APA (6th Edition):
Mullen, A. R. (2013). Reductive Carboxylation Is a Novel Pathway of Glutamine Metabolism That Supports the Growth of Tumor Cells with Metabolic Defects. (Thesis). University of Texas Southwestern Medical Center. Retrieved from http://hdl.handle.net/2152.5/2726
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Mullen, Andrew Robbins. “Reductive Carboxylation Is a Novel Pathway of Glutamine Metabolism That Supports the Growth of Tumor Cells with Metabolic Defects.” 2013. Thesis, University of Texas Southwestern Medical Center. Accessed March 01, 2021.
http://hdl.handle.net/2152.5/2726.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Mullen, Andrew Robbins. “Reductive Carboxylation Is a Novel Pathway of Glutamine Metabolism That Supports the Growth of Tumor Cells with Metabolic Defects.” 2013. Web. 01 Mar 2021.
Vancouver:
Mullen AR. Reductive Carboxylation Is a Novel Pathway of Glutamine Metabolism That Supports the Growth of Tumor Cells with Metabolic Defects. [Internet] [Thesis]. University of Texas Southwestern Medical Center; 2013. [cited 2021 Mar 01].
Available from: http://hdl.handle.net/2152.5/2726.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Mullen AR. Reductive Carboxylation Is a Novel Pathway of Glutamine Metabolism That Supports the Growth of Tumor Cells with Metabolic Defects. [Thesis]. University of Texas Southwestern Medical Center; 2013. Available from: http://hdl.handle.net/2152.5/2726
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
University of Western Ontario
4.
Aldhafeeri, Hamad.
CRISPR Screen for Identification of Kinases that Mediate Prostate Cancer Cell Invasion.
Degree: 2017, University of Western Ontario
URL: https://ir.lib.uwo.ca/etd/5205
► Metastasis is the primary cause of mortality in cancer patients. Inhibition of proteins that are involved in the regulation of metastasis are expected to suppress…
(more)
▼ Metastasis is the primary cause of mortality in cancer patients. Inhibition of proteins that are involved in the regulation of metastasis are expected to suppress metastasis and represent treatment targets. Our focus is on prostate cancer metastasis and we have developed a novel high-throughput means of performing in vitro screens for regulators of prostate cancer metastasis. We propose to use a focused CRISPR library screen that will “knock out” all human kinases to determine which ones are responsible for prostate cancer metastasis. CRISPR is a potent genetic editing tool and was used to silence all kinases in prostate cells (BPH cells). These cells form spheroid colonies in 3D culture and were subjected to a CRISPR kinase screen. We observed a morphological change in some colonies to a stellate morphology, which represents aggressive behavior. These stellate colonies were then isolated to identify the responsible kinase. We identified Glycogen synthase kinase 3 beta (GSK3ß) as a potential regulator for prostate cancer cell morphology and possible, metastasis.
Subjects/Keywords: Medical Pathology; Neoplasms
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APA (6th Edition):
Aldhafeeri, H. (2017). CRISPR Screen for Identification of Kinases that Mediate Prostate Cancer Cell Invasion. (Thesis). University of Western Ontario. Retrieved from https://ir.lib.uwo.ca/etd/5205
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Aldhafeeri, Hamad. “CRISPR Screen for Identification of Kinases that Mediate Prostate Cancer Cell Invasion.” 2017. Thesis, University of Western Ontario. Accessed March 01, 2021.
https://ir.lib.uwo.ca/etd/5205.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Aldhafeeri, Hamad. “CRISPR Screen for Identification of Kinases that Mediate Prostate Cancer Cell Invasion.” 2017. Web. 01 Mar 2021.
Vancouver:
Aldhafeeri H. CRISPR Screen for Identification of Kinases that Mediate Prostate Cancer Cell Invasion. [Internet] [Thesis]. University of Western Ontario; 2017. [cited 2021 Mar 01].
Available from: https://ir.lib.uwo.ca/etd/5205.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Aldhafeeri H. CRISPR Screen for Identification of Kinases that Mediate Prostate Cancer Cell Invasion. [Thesis]. University of Western Ontario; 2017. Available from: https://ir.lib.uwo.ca/etd/5205
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
5.
VanBeckum, Danielle R.
CHARACTERIZATION OF HCMV-ENCODED CHEMOKINE RECEPTOR US28 TRANSDUCED MSU1.1 HUMAN FIBROBLAST CELLS.
Degree: MS, Biology, 2014, Northen Michigan University
URL: https://commons.nmu.edu/theses/24
► Human Cytomegalovirus (HCMV) encodes the G-protein coupled receptor US28. Using a mouse model system, US28 was previously found to be oncomodulatory, increasing proliferation, inducing…
(more)
▼ Human Cytomegalovirus (HCMV) encodes the G-protein coupled receptor US28. Using a mouse model system, US28 was previously found to be oncomodulatory, increasing proliferation, inducing anchorage independent growth and loss of contact inhibition. Similarly, in the human glioblastoma cell line U373, US28 activated VEGF expression. To determine if US28 is oncomodulatory in normal human cells, we engineered the human fibroblast cell line MSU1.1 to express US28 via lentivirus infection. MSU1.1 cells were transduced with pHAGE-US28-ZsGreen. Fluorescent confocal microscopy was utilized to detect the ZsGreen tag and confirmed the successful transduction of MSU1.1 cells with the US28 vector or empty pHAGE vector. MSU1.1 cells expressing US28 did not exhibit loss of contact inhibition nor anchorage independent growth in focus formation and soft agar assays, respectively. Using the AlamarBlue assay, US28-expressing cells showed a 2.303 fold increase in proliferation compared to wild type MSU1.1 cells and a 2.201 fold increase compared to mock transduced cells. US28 expression in MSU1.1 cell line increased proliferation but did not trigger a transformed phenotype. This study reveals potential cell-type and species-specific differences from previous findings assessing US28 expression in NIH-3T3 (mouse fibroblast) and U373 (malignant glioma) cell lines. The reported prevalence of US28 in GBM and confirmation of oncomodulation via increased proliferation in MSU1.1 cells makes US28 a potential target warranting further understanding of the virus’ role in GBM.
Advisors/Committee Members: Dr. Robert J. Winn, Ph.D..
Subjects/Keywords: Neoplasms; Virus Diseases
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APA ·
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APA (6th Edition):
VanBeckum, D. R. (2014). CHARACTERIZATION OF HCMV-ENCODED CHEMOKINE RECEPTOR US28 TRANSDUCED MSU1.1 HUMAN FIBROBLAST CELLS. (Thesis). Northen Michigan University. Retrieved from https://commons.nmu.edu/theses/24
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
VanBeckum, Danielle R. “CHARACTERIZATION OF HCMV-ENCODED CHEMOKINE RECEPTOR US28 TRANSDUCED MSU1.1 HUMAN FIBROBLAST CELLS.” 2014. Thesis, Northen Michigan University. Accessed March 01, 2021.
https://commons.nmu.edu/theses/24.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
VanBeckum, Danielle R. “CHARACTERIZATION OF HCMV-ENCODED CHEMOKINE RECEPTOR US28 TRANSDUCED MSU1.1 HUMAN FIBROBLAST CELLS.” 2014. Web. 01 Mar 2021.
Vancouver:
VanBeckum DR. CHARACTERIZATION OF HCMV-ENCODED CHEMOKINE RECEPTOR US28 TRANSDUCED MSU1.1 HUMAN FIBROBLAST CELLS. [Internet] [Thesis]. Northen Michigan University; 2014. [cited 2021 Mar 01].
Available from: https://commons.nmu.edu/theses/24.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
VanBeckum DR. CHARACTERIZATION OF HCMV-ENCODED CHEMOKINE RECEPTOR US28 TRANSDUCED MSU1.1 HUMAN FIBROBLAST CELLS. [Thesis]. Northen Michigan University; 2014. Available from: https://commons.nmu.edu/theses/24
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
6.
Santos, Ingrid da Silva [UNESP].
Absence of human papillomavirus in fresh tissue of oral cavity and oropharynx cancer in patients from the northwest region of São Paulo, Brazil.
Degree: 2017, Universidade Estadual Paulista
URL: http://hdl.handle.net/11449/150980
► Evidências sugerem que o papilomavírus humano (HPV) está associado com um subgrupo de carcinomas de células escamosas da cabeça e pescoço (HNSCC). No entanto, a…
(more)
▼ Evidências sugerem que o papilomavírus humano (HPV) está associado com um subgrupo de carcinomas de células escamosas da cabeça e pescoço (HNSCC). No entanto, a prevalência do HPV varia substancialmente dependendo do local anatômico e da região geográfica estudada. Aqui, nosso objetivo foi investigar a prevalência do HPV em amostras de tecido fresco de pacientes brasileiros com carcinoma de células escamosas (CEC) de boca e orofaringe combinando dois métodos confiáveis para a detecção do HPV. Foram recrutadas trinta e seis amostras de tecido fresco provenientes de CEC de boca (n= 27) e orofaringe (n= 9) para análises. As características sociodemográficas, estilo de vida e clinicopatológicas foram coletadas através dos prontuários. O DNA do HPV foi detectado por dois métodos: reação em cadeia da polimerase (PCR) em tempo real através de ensaio qualitativo de presença ou ausência do HPV-16, e testado para 37 genótipos usando Linear Array. A amplificação do gene β -globina funcionou como controle interno positivo para a análise do DNA em todas as amostras. O DNA do HPV não foi detectado em nenhum dos casos de amostras de tecido de pacientes com CEC em ambos os métodos utilizados. A ausência do HPV observada em nosso estudo pode sugerir que este não é um fator de risco prevalente nos CECs de boca e orofaringe nesta região geográfica. Os fatores de risco clássicos para o desenvolvimento desses tumores parecem ser ainda a principal causa nessa população brasileira. Investigações detalhadas do estilo de vida com maior amostragem precisam ser melhor exploradas para compreensão da baixa prevalência encontrada.
Evidence suggests that human papillomavirus (HPV) is associated with a subgroup of squamous cell carcinomas of the head and neck (HNSCC). However, the prevalence of HPV varies substantially depending on the anatomical site and geographic region studied. Here, our goal was to investigate the prevalence of HPV in fresh tissue samples from Brazilian patients with squamous cell carcinoma (SCC) of the oral cavity and oropharynx by combining two reliable methods for the detection of the HPV DNA. We recruited thirty-six fresh tissue samples from SCC of the oral cavity (n= 27) and oropharynx (n= 9) for analysis. The sociodemographic, lifestyle and clinicopathological characteristics were obtained from individual medical records. HPV DNA was detected by two methods: real-time polymerase chain reaction (PCR) through the qualitative assay of presence or absence for HPV-16, and tested for 37 genotypes by the Roche Linear Array. Amplification of the β-globin gene functioned as a positive internal control for DNA analysis in all samples. HPV DNA was detected in none of the tissue samples from patients with SCC in both methods. The absence of HPV observed in our study may suggest that this is not a prevalent risk factor in SCC of the oral cavity and oropharynx in this geographical region. The classic risk factors for the development of these tumors seem to be still the main cause in this Brazilian population. Detailed…
Advisors/Committee Members: Miyahara, Glauco Issamu [UNESP], Bernabé, Daniel Galera [UNESP], Oliveira, Sandra Helena Penha de [UNESP], Universidade Estadual Paulista (UNESP).
Subjects/Keywords: Papillomaviridae; Oropharyngeal neoplasms; Head and neck neoplasms; Mouth neoplasms
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APA (6th Edition):
Santos, I. d. S. [. (2017). Absence of human papillomavirus in fresh tissue of oral cavity and oropharynx cancer in patients from the northwest region of São Paulo, Brazil. (Thesis). Universidade Estadual Paulista. Retrieved from http://hdl.handle.net/11449/150980
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Santos, Ingrid da Silva [UNESP]. “Absence of human papillomavirus in fresh tissue of oral cavity and oropharynx cancer in patients from the northwest region of São Paulo, Brazil.” 2017. Thesis, Universidade Estadual Paulista. Accessed March 01, 2021.
http://hdl.handle.net/11449/150980.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Santos, Ingrid da Silva [UNESP]. “Absence of human papillomavirus in fresh tissue of oral cavity and oropharynx cancer in patients from the northwest region of São Paulo, Brazil.” 2017. Web. 01 Mar 2021.
Vancouver:
Santos IdS[. Absence of human papillomavirus in fresh tissue of oral cavity and oropharynx cancer in patients from the northwest region of São Paulo, Brazil. [Internet] [Thesis]. Universidade Estadual Paulista; 2017. [cited 2021 Mar 01].
Available from: http://hdl.handle.net/11449/150980.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Santos IdS[. Absence of human papillomavirus in fresh tissue of oral cavity and oropharynx cancer in patients from the northwest region of São Paulo, Brazil. [Thesis]. Universidade Estadual Paulista; 2017. Available from: http://hdl.handle.net/11449/150980
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
University of Sydney
7.
Tracey, Elizabeth Ann.
Cancer survival in New South Wales (NSW) and the impact of distance from and access to cancer surgical services: a data linkage study
.
Degree: 2015, University of Sydney
URL: http://hdl.handle.net/2123/12548
► Cancer survival is poorer in rural NSW but specialist cancer surgical services are predominately located in the Sydney region. The aim of this thesis is…
(more)
▼ Cancer survival is poorer in rural NSW but specialist cancer surgical services are predominately located in the Sydney region. The aim of this thesis is to examine whether increasing distance from cancer surgical services affects access to those services and ultimately cancer survival. The method used was population based data linkage for patients diagnosed between 2000 and 2008 (data obtained from the NSW Central Cancer Registry database and linked to hospitalisations) and followed to the end of 2008. Distance was measured in kilometres from a person’s home to their hospital of surgery (bladder cancer) or the closest specialist hospital (ovarian and lung cancer) by using geographical coordinates. Associations were modelled using logistic regression and the hazard of death using Cox regression and the survival time parametric method (stpm2). The hazard of death decreased with distance for people with bladder cancer who had a cystectomy. People with ovarian and lung cancer who lived further from specialist surgical hospitals were much more likely to attend general hospitals, have advanced or unknown stage cancer at diagnosis and have limited or no surgery. Understanding the factors that impede referral to specialist surgical hospitals particularly those who live remotely is essential for optimal cancer survival.
Subjects/Keywords: Bladder neoplasms;
Lung neoplasms;
Ovarian neoplasms;
Accessibility of services;
Surgery distance
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APA (6th Edition):
Tracey, E. A. (2015). Cancer survival in New South Wales (NSW) and the impact of distance from and access to cancer surgical services: a data linkage study
. (Thesis). University of Sydney. Retrieved from http://hdl.handle.net/2123/12548
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Tracey, Elizabeth Ann. “Cancer survival in New South Wales (NSW) and the impact of distance from and access to cancer surgical services: a data linkage study
.” 2015. Thesis, University of Sydney. Accessed March 01, 2021.
http://hdl.handle.net/2123/12548.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Tracey, Elizabeth Ann. “Cancer survival in New South Wales (NSW) and the impact of distance from and access to cancer surgical services: a data linkage study
.” 2015. Web. 01 Mar 2021.
Vancouver:
Tracey EA. Cancer survival in New South Wales (NSW) and the impact of distance from and access to cancer surgical services: a data linkage study
. [Internet] [Thesis]. University of Sydney; 2015. [cited 2021 Mar 01].
Available from: http://hdl.handle.net/2123/12548.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Tracey EA. Cancer survival in New South Wales (NSW) and the impact of distance from and access to cancer surgical services: a data linkage study
. [Thesis]. University of Sydney; 2015. Available from: http://hdl.handle.net/2123/12548
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
East Carolina University
8.
Corbin, Joshua Moses.
TMEFF2 is an epigenetic modulator that promotes androgen independent growth in castration-resistant prostate cancer cells.
Degree: MS, Biomedical Sciences, 2014, East Carolina University
URL: http://hdl.handle.net/10342/4662
► While the ability to detect PCa has improved significantly due to PSA screenings, the survival rate for men diagnosed with PCa has remained stagnant, and…
(more)
▼ While the ability to detect PCa has improved significantly due to PSA screenings, the survival rate for men diagnosed with PCa has remained stagnant, and the disease remains the second leading cause of cancer related deaths in men. Most patients initially respond to androgen deprivation treatment; however, a significant percentage of patients relapse with currently untreatable castration resistant prostate cancer (CRPC), during which the PCa cells develop the ability to grow in androgen depleted conditions. The androgen receptor (AR) plays a vital role in prostate development and homeostasis, and the deregulation of AR drives PCa tumorigenesis and progression to CRPC. Delineating molecular mechanisms that contribute to AR activity and/or PCa cell growth in androgen-depleted conditions may aid in the development of future CRPC therapies Epigenetic alterations play a critical role in differentiation during development, and aberrations in epigenetic regulation are associated with tumorigenesis and cancer progression. Two types of epigenetic modifications, DNA methylation and the methylation of multiple histone lysines, play significant roles in prostate cancer (PCa). Many histone methyltransferases (HMT) and demethylases (HDM), including the JMJD2 family of histone demethyalses, act as coregulators of AR, and many of these enzymes are implicated in CRPC. Because of this, HDMSs and HMTs have proven as attractive targets for therapeutic intervention. We have been studying TMEFF2, a protein that is regulated transcriptionally and translationally by the AR, and is overexpressed in PCa and CRPC suggesting a role in this disease. Data presented here demonstrate that TMEFF2 modulates JMJD2 controlled methyl histone marks and increases growth in androgen depleted conditions in CRPC cells. In correlation with its effect on histone methylation and growth, TMEFF2 overexpression increases resistance to the anti-growth effects of the pan-jumonji demethylase inhibitor, JIB-04, suggesting that TMEFF2 modulates growth, at least in part, by increasing jumonji demethylase activity. Additionally, TMEFF2 positively regulates PSA expression without altering AR levels in CRPC cells, indicating that TMEFF2 is a novel activator of AR. All together this data suggests a model in which TMEFF2, by modulating the activity of AR and JMJD2 enzymes, increases CRPC cell growth. Because CRPC remains to be a significant obstacle in the successful treatment of metastatic PCa, the results presented have the potential to be of therapeutic value.
Advisors/Committee Members: Ruiz-Echevarria, Maria (advisor).
Subjects/Keywords: Oncology;
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APA ·
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CSE |
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Manager
APA (6th Edition):
Corbin, J. M. (2014). TMEFF2 is an epigenetic modulator that promotes androgen independent growth in castration-resistant prostate cancer cells. (Masters Thesis). East Carolina University. Retrieved from http://hdl.handle.net/10342/4662
Chicago Manual of Style (16th Edition):
Corbin, Joshua Moses. “TMEFF2 is an epigenetic modulator that promotes androgen independent growth in castration-resistant prostate cancer cells.” 2014. Masters Thesis, East Carolina University. Accessed March 01, 2021.
http://hdl.handle.net/10342/4662.
MLA Handbook (7th Edition):
Corbin, Joshua Moses. “TMEFF2 is an epigenetic modulator that promotes androgen independent growth in castration-resistant prostate cancer cells.” 2014. Web. 01 Mar 2021.
Vancouver:
Corbin JM. TMEFF2 is an epigenetic modulator that promotes androgen independent growth in castration-resistant prostate cancer cells. [Internet] [Masters thesis]. East Carolina University; 2014. [cited 2021 Mar 01].
Available from: http://hdl.handle.net/10342/4662.
Council of Science Editors:
Corbin JM. TMEFF2 is an epigenetic modulator that promotes androgen independent growth in castration-resistant prostate cancer cells. [Masters Thesis]. East Carolina University; 2014. Available from: http://hdl.handle.net/10342/4662
University of Utah
9.
Qian, Lian.
Molecular design and chemical synthesis of affinity probes for lysophosphatidic acid receptors;.
Degree: PhD, Medicinal Chemistry;, 2004, University of Utah
URL: http://content.lib.utah.edu/cdm/singleitem/collection/etd1/id/810/rec/840
► Lysophosphatidic acid (LPA) is an important phospholipid that mediates a variety of biologic effects. LPA induces cell proliferation, morphological changes, and has been shown to…
(more)
▼ Lysophosphatidic acid (LPA) is an important phospholipid that mediates a variety of biologic effects. LPA induces cell proliferation, morphological changes, and has been shown to be involved in many pathophysiological processes including cancers, immunological diseases, and cardiovascular diseases and wound healing. LPA accomplishes its biological activities by acting mainly upon G-protein coupled receptors. These receptors exist in most of mammalian cells and each receptor's functions are still not clear. Thus, development of approaches to modify LPA function could have broad applicability in the control and improvement of pathophysiological conditions. LPA is present in ascites from ovarian cancer patients at very high concentrations, and it plays an essential role in regulating drug sensitivity and invasiveness of ovarian cancer cells. The development of antibodies against LPA has the potential to improve the diagnosis and treatment of ovarian cancer. In the first study, I synthesized lipid immunogens as bioconjugates with different linkers and carriers for preparation of specific antisera. A LPA/PA cross-reactive murine IgG mAb was generated, which will be useful to selectively extract LPA and PA molecular species from human serum. In a separate study, I synthesized a series of long-lived and metabolically stabilized LPA analogues. Hydroxyethoxy (HE)-substituted LPA derivatives selectively activated the LP A3 receptor, but with a potency tenfold lower than natural oleoyl LPA. A methyl thiophosphate LPA analogue, OMPT (l-oleoyl-2-O-methyl-rflc-glycero-phosphothioate), showed enantioselective responses to LPA3 receptor, and a highly potent and selective LP A3 receptor agonist (2.S)-0MPT was discovered. Among a group of thiophosphate LPA analogues with ether chains, two phosphodiesters, LQIO and LQll, were found to act as antagonists to inhibit the stimulatory effects of LPA-mediated signaling on DNA synthesis in cells. The synthesis of these compounds provides tremendous insight into how LPA receptor antagonists with high potency and less toxicity can be generated as potential therapeutics for the treatment of ovarian cancer.
Subjects/Keywords: Ovarian Neoplasms
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APA ·
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Export
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Manager
APA (6th Edition):
Qian, L. (2004). Molecular design and chemical synthesis of affinity probes for lysophosphatidic acid receptors;. (Doctoral Dissertation). University of Utah. Retrieved from http://content.lib.utah.edu/cdm/singleitem/collection/etd1/id/810/rec/840
Chicago Manual of Style (16th Edition):
Qian, Lian. “Molecular design and chemical synthesis of affinity probes for lysophosphatidic acid receptors;.” 2004. Doctoral Dissertation, University of Utah. Accessed March 01, 2021.
http://content.lib.utah.edu/cdm/singleitem/collection/etd1/id/810/rec/840.
MLA Handbook (7th Edition):
Qian, Lian. “Molecular design and chemical synthesis of affinity probes for lysophosphatidic acid receptors;.” 2004. Web. 01 Mar 2021.
Vancouver:
Qian L. Molecular design and chemical synthesis of affinity probes for lysophosphatidic acid receptors;. [Internet] [Doctoral dissertation]. University of Utah; 2004. [cited 2021 Mar 01].
Available from: http://content.lib.utah.edu/cdm/singleitem/collection/etd1/id/810/rec/840.
Council of Science Editors:
Qian L. Molecular design and chemical synthesis of affinity probes for lysophosphatidic acid receptors;. [Doctoral Dissertation]. University of Utah; 2004. Available from: http://content.lib.utah.edu/cdm/singleitem/collection/etd1/id/810/rec/840
Universidade Estadual de Campinas
10.
Marcello, Marjory Alana, 1986-.
Estudo molecular da adiponectina, grelina, leptina e resistina : estabelecendo as ligações entre a obesidade e o câncer de tireoide: Molecular profile of adiponectin, ghrelin, leptin and resistin : esteblishing the links between obesity and thyroid cancer.
Degree: 2015, Universidade Estadual de Campinas
URL: http://repositorio.unicamp.br/jspui/handle/REPOSIP/310266
► Abstract: The incidence of obesity and of differentiated thyroid cancer (DTC) has increased exponentially over the world and there are strong indications that these two…
(more)
▼ Abstract: The incidence of obesity and of differentiated thyroid cancer (DTC) has increased exponentially over the world and there are strong indications that these two conditions are combined. Several studies have shown that adipokines such as adiponectin, resistin, leptin and ghrelin may be the link between these diseases. The objective of this investigation was to study the molecular profile of adipokines: adiponectin, leptin, resistin and ghrelin and its relationship with the anthropometric features of patients with various types of thyroid nodules in order to investigate the link between obesity and
neoplasms and identify possible markers of diagnosis, evolution and prognosis for DTC. A group of patients with DTC, patients with benign thyroid nodules and healthy control subjects were interviewed about their eating habits and routine, and went through a complete anthropometric assessment. The serum concentrations of adiponectin, leptin, resistin, and ghrelin were measured by ELISA. Patients were genotyped for 21 SNPs in genes encoding adipokines and their receptors using Taqman SNP Genotyping technique. In addition, surgical specimens were investigated for mRNA of these adipokines. We demonstrated that being overweight increases the risk of developing CDT almost 4 times and this risk may increase when there is excess intake of calories associated with excessive consumption of carbohydrates and proteins. Serum concentrations of adipokines were excellent markers of nodules malignancy diagnosis, with a 100% accuracy using leptin and resistin. The genetic inheritance of polymorphisms rs7799039 in LEP rs1137101 and rs2232165 in LEPR in GHSR increased the risk of developing CDT 4, 11 and 22 times, respectively. In addition, SNPs heritage in LEP (rs7799039, rs2167270), ADIPOQ - rs12629945, ADIPOR1 - rs2232853 and rs1342387, ADIPOR2 - rs1058322 and GHSR - rs2232165 altered sérum concentrations of leptin, adiponectin and ghrelin. Also, we showed that although the thyroid cell is not the main producer of adipokines, adiponectin, leptin, and ghrelin resitina are produced by thyroid câncer cells, findng mRNA for all cytokines in the tissues analyzed, suggesting that they play an important role in tumorigenesis or thyroid tumor proliferation. We conclude that individuals with thyroid nodules, especially individuals with DTC have diferente anthropometric and molecular adipokine profiles that increases the susceptibility to DTC as well as genetic changes and / or expression of adiponectin, leptin, resistin and ghrelin. We suggest that it is possible to draw a CDT carrier Individual profile for body constitution, eating habits and molecular profile of adiponectin, leptin, resistin and ghrelin may identify individuals at risk for DTC, and are a potential link between obesity, inflammation, and DTC
Advisors/Committee Members: UNIVERSIDADE ESTADUAL DE CAMPINAS (CRUESP), Ward, Laura Sterian, 1956- (advisor), Universidade Estadual de Campinas. Faculdade de Ciências Médicas (institution), Programa de Pós-Graduação em Clínica Médica (nameofprogram), Toro, Ivan Felizardo Contrera (committee member), Schenka, André Almeida (committee member), Ferreira, Rita de Cassia (committee member), Mazeto, Glaucia Maria Ferreira da Silva (committee member).
Subjects/Keywords: Tireóide; Neoplasias; Thyroid gland; Neoplasms
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APA ·
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Export
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APA (6th Edition):
Marcello, Marjory Alana, 1. (2015). Estudo molecular da adiponectina, grelina, leptina e resistina : estabelecendo as ligações entre a obesidade e o câncer de tireoide: Molecular profile of adiponectin, ghrelin, leptin and resistin : esteblishing the links between obesity and thyroid cancer. (Thesis). Universidade Estadual de Campinas. Retrieved from http://repositorio.unicamp.br/jspui/handle/REPOSIP/310266
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Marcello, Marjory Alana, 1986-. “Estudo molecular da adiponectina, grelina, leptina e resistina : estabelecendo as ligações entre a obesidade e o câncer de tireoide: Molecular profile of adiponectin, ghrelin, leptin and resistin : esteblishing the links between obesity and thyroid cancer.” 2015. Thesis, Universidade Estadual de Campinas. Accessed March 01, 2021.
http://repositorio.unicamp.br/jspui/handle/REPOSIP/310266.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Marcello, Marjory Alana, 1986-. “Estudo molecular da adiponectina, grelina, leptina e resistina : estabelecendo as ligações entre a obesidade e o câncer de tireoide: Molecular profile of adiponectin, ghrelin, leptin and resistin : esteblishing the links between obesity and thyroid cancer.” 2015. Web. 01 Mar 2021.
Vancouver:
Marcello, Marjory Alana 1. Estudo molecular da adiponectina, grelina, leptina e resistina : estabelecendo as ligações entre a obesidade e o câncer de tireoide: Molecular profile of adiponectin, ghrelin, leptin and resistin : esteblishing the links between obesity and thyroid cancer. [Internet] [Thesis]. Universidade Estadual de Campinas; 2015. [cited 2021 Mar 01].
Available from: http://repositorio.unicamp.br/jspui/handle/REPOSIP/310266.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Marcello, Marjory Alana 1. Estudo molecular da adiponectina, grelina, leptina e resistina : estabelecendo as ligações entre a obesidade e o câncer de tireoide: Molecular profile of adiponectin, ghrelin, leptin and resistin : esteblishing the links between obesity and thyroid cancer. [Thesis]. Universidade Estadual de Campinas; 2015. Available from: http://repositorio.unicamp.br/jspui/handle/REPOSIP/310266
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Universidade Estadual de Campinas
11.
Pereira, Manoela Carrera Martinez Cavalcante, 1982-.
Avaliação do papel biológico do gene homeobox HOXA10 em carcinomas espinocelulares orais: Biological role of HOXA10 homeobox gene in oral squamous cell carcinoma.
Degree: 2012, Universidade Estadual de Campinas
URL: http://repositorio.unicamp.br/jspui/handle/REPOSIP/288739
► Abstract: Although HOX genes are known for acting in the regulation of important events during embryogenesis, such as cellular proliferation, differentiation and migration, alterations in…
(more)
▼ Abstract: Although HOX genes are known for acting in the regulation of important events during embryogenesis, such as cellular proliferation, differentiation and migration, alterations in their expression patterns have been frequently associated to the development of cancers. Studies in our laboratory characterized the expression profile of the 39 members of the HOX family of homeobox genes in oral samples of normal mucosa and squamous cell carcinoma (SCC), identifying differently expressed genes. Among those genes are HOXA10, which has its expression related to tumor development and prognosis. The aim of the study was to valitade the elevated levels of HOXA10 on oral SCCs comparing to the normal oral mucosa, and to analyze the effects of the overexpression and neutralization of HOXA10 in modulating the main biological events associated to tumorigenesis. The levels of HOXA10 were evaluated by immunohistochemistry and qRT-PCR, and the HOXA10 effects on proliferation, apoptosis, adhesion, epitelial-mesenchimal transition (EMT), migration and invasion were evaluated on HaCaT normal keratinocytes cells overexpressing HOXA10 and on HSC-3 tongue carcinoma cells expressing a shRNA sequence to neutralize HOXA10 expression. The expression of HOXA10 was significantly higher on oral SCC samples when compared to the normal tissue controls. HaCaT cells overexpressing HOXA10 showed higher expression of N-cadherin and ?-catenin mRNA levels, and adhesion and migration were coordinately regulated on those cells. The neutralization of HOXA10 reduced significantly the proliferation capacity of HSC-3 cells, while induced significantly the expression of EMT markers, cell adhesion as well as the migration and invasion of HSC-3 cells. Overexpression and neutralization of HOXA10 did not modulate apoptosis rates. In conclusion, the results of this study suggest that the HOXA10 expression modulates important events associated with development and progression of oral SCCs
Advisors/Committee Members: UNIVERSIDADE ESTADUAL DE CAMPINAS (CRUESP), Della Coletta, Ricardo, 1972- (advisor), Salo, Tuula Anneli (coadvisor), Universidade Estadual de Campinas. Faculdade de Odontologia de Piracicaba (institution), Programa de Pós-Graduação em Estomatopatologia (nameofprogram), Ribeiro, Ana Carolina Prado (committee member), Reis, Silvia Regina de Almeida (committee member), Marques, Marcelo Rocha (committee member), Lopes, Márcio Ajudarte (committee member).
Subjects/Keywords: Neoplasias bucais; Mouth Neoplasms
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APA ·
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APA (6th Edition):
Pereira, Manoela Carrera Martinez Cavalcante, 1. (2012). Avaliação do papel biológico do gene homeobox HOXA10 em carcinomas espinocelulares orais: Biological role of HOXA10 homeobox gene in oral squamous cell carcinoma. (Thesis). Universidade Estadual de Campinas. Retrieved from http://repositorio.unicamp.br/jspui/handle/REPOSIP/288739
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Pereira, Manoela Carrera Martinez Cavalcante, 1982-. “Avaliação do papel biológico do gene homeobox HOXA10 em carcinomas espinocelulares orais: Biological role of HOXA10 homeobox gene in oral squamous cell carcinoma.” 2012. Thesis, Universidade Estadual de Campinas. Accessed March 01, 2021.
http://repositorio.unicamp.br/jspui/handle/REPOSIP/288739.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Pereira, Manoela Carrera Martinez Cavalcante, 1982-. “Avaliação do papel biológico do gene homeobox HOXA10 em carcinomas espinocelulares orais: Biological role of HOXA10 homeobox gene in oral squamous cell carcinoma.” 2012. Web. 01 Mar 2021.
Vancouver:
Pereira, Manoela Carrera Martinez Cavalcante 1. Avaliação do papel biológico do gene homeobox HOXA10 em carcinomas espinocelulares orais: Biological role of HOXA10 homeobox gene in oral squamous cell carcinoma. [Internet] [Thesis]. Universidade Estadual de Campinas; 2012. [cited 2021 Mar 01].
Available from: http://repositorio.unicamp.br/jspui/handle/REPOSIP/288739.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Pereira, Manoela Carrera Martinez Cavalcante 1. Avaliação do papel biológico do gene homeobox HOXA10 em carcinomas espinocelulares orais: Biological role of HOXA10 homeobox gene in oral squamous cell carcinoma. [Thesis]. Universidade Estadual de Campinas; 2012. Available from: http://repositorio.unicamp.br/jspui/handle/REPOSIP/288739
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Universidade Estadual de Campinas
12.
Rocha, Guilherme Zweig, 1983-.
A atuação da proteína quinase dependente de dsRNA (PKR) no desenvolvimento de tumor de cólon em camundongos obesos: The role of dsRNA dependent protein kinase (PKR) on colon tumor development in obese mice.
Degree: 2014, Universidade Estadual de Campinas
URL: http://repositorio.unicamp.br/jspui/handle/REPOSIP/312750
► Abstract: Although obesity is recognized as a major cause of diabetes and cardiovascular disease, the association between obesity and different types of cancer has received…
(more)
▼ Abstract: Although obesity is recognized as a major cause of diabetes and cardiovascular disease, the association between obesity and different types of cancer has received much less attention. The association between obesity and the development of colon cancer is one of the major conceptual advances in the pathogenesis of colon cancer in the last decade. Recently the role of subclinical inflammation in obesity and in carcinogenesis gained prominence. Mechanistically it is believed that obesity acts as a tumor promoter, and their pro-tumorigenic effects depend mainly on low-grade inflammatory response caused by obesity, involving the production of inflammatory and pro-tumorigenic cytokines (TNF and IL-6). A key feature of obesity-induced inflammation is the infiltration of macrophages in adipose tissue, producing inflammatory cytokines and other mediators that interfere with insulin signaling. Reticulum stress and inflammation are connected on many levels and work as short period adaptive systems required for the function and survival of the organism, and both are detrimental when chronically activated. In this regard, the activation of PKR during inflammation and subsequent activation of JNK by PKR also interferes and impairs insulin signaling pathway. Thus, PKR can form a metabolically active inflammatory complex which then becomes part of the of insulin pathway and of the pathogens response pathway and control of translation sensible to nutrients. The relationship between colon cancer and obesity may be due to action at the molecular level, subclinical low-grade inflammation and cellular stress caused by this inflammatory signaling. PKR is responsive to inflammatory signaling and also to the insulin pathway in other tissues, and related to carcinogenesis and progression in several types of cancer. Thus, investigation of it's participation is relevant as it provides the understanding of the molecular pathophysiology of colon tumors. Thus, the main objective of the study was to evaluate the role of PKR in the development of colon tumors in mice subjected to a high-fat diet. The absence of PKR prevents the formation of tumors. Moreover, apparently the absence of PKR in myeloid cells also confers protection against resistance to insulin induced by a high-fat diet, reducing inflammation induced by obesity. These observations demonstrate that PKR can be a primary point during carcinogenesis associated with inflammation and may represent a promising target for therapeutic intervention
Advisors/Committee Members: UNIVERSIDADE ESTADUAL DE CAMPINAS (CRUESP), Carvalheira, José Barreto Campello, 1971- (advisor), Universidade Estadual de Campinas. Faculdade de Ciências Médicas (institution), Programa de Pós-Graduação em Fisiopatologia Médica (nameofprogram), Reis, Leonardo Oliveira (committee member), Yunes, José Andrés (committee member), Ferreira, Carmen Veríssima (committee member), Chammas, Roger (committee member), Folli, Franco Battista Ennio (committee member).
Subjects/Keywords: Câncer; Inflamação; Inflammation; Neoplasms
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APA (6th Edition):
Rocha, Guilherme Zweig, 1. (2014). A atuação da proteína quinase dependente de dsRNA (PKR) no desenvolvimento de tumor de cólon em camundongos obesos: The role of dsRNA dependent protein kinase (PKR) on colon tumor development in obese mice. (Thesis). Universidade Estadual de Campinas. Retrieved from http://repositorio.unicamp.br/jspui/handle/REPOSIP/312750
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Rocha, Guilherme Zweig, 1983-. “A atuação da proteína quinase dependente de dsRNA (PKR) no desenvolvimento de tumor de cólon em camundongos obesos: The role of dsRNA dependent protein kinase (PKR) on colon tumor development in obese mice.” 2014. Thesis, Universidade Estadual de Campinas. Accessed March 01, 2021.
http://repositorio.unicamp.br/jspui/handle/REPOSIP/312750.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Rocha, Guilherme Zweig, 1983-. “A atuação da proteína quinase dependente de dsRNA (PKR) no desenvolvimento de tumor de cólon em camundongos obesos: The role of dsRNA dependent protein kinase (PKR) on colon tumor development in obese mice.” 2014. Web. 01 Mar 2021.
Vancouver:
Rocha, Guilherme Zweig 1. A atuação da proteína quinase dependente de dsRNA (PKR) no desenvolvimento de tumor de cólon em camundongos obesos: The role of dsRNA dependent protein kinase (PKR) on colon tumor development in obese mice. [Internet] [Thesis]. Universidade Estadual de Campinas; 2014. [cited 2021 Mar 01].
Available from: http://repositorio.unicamp.br/jspui/handle/REPOSIP/312750.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Rocha, Guilherme Zweig 1. A atuação da proteína quinase dependente de dsRNA (PKR) no desenvolvimento de tumor de cólon em camundongos obesos: The role of dsRNA dependent protein kinase (PKR) on colon tumor development in obese mice. [Thesis]. Universidade Estadual de Campinas; 2014. Available from: http://repositorio.unicamp.br/jspui/handle/REPOSIP/312750
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
University of Texas Southwestern Medical Center
13.
Adams, Cassandra Leigh.
Attachment Influences within a Gynecologic Cancer Population.
Degree: 2012, University of Texas Southwestern Medical Center
URL: http://hdl.handle.net/2152.5/1212
► Despite significant levels of distress and demonstrated benefits of psychosocial intervention, few women diagnosed with gynecologic cancers utilize psychosocial resources. Research indicates adult attachment style…
(more)
▼ Despite significant levels of distress and demonstrated benefits of psychosocial intervention, few women diagnosed with gynecologic cancers utilize psychosocial resources. Research indicates adult attachment style and perception of social support impact distress. However, relationships between these variables are poorly understood. Participants completed measures of distress, adult attachment style, and perception of social support and provided information regarding self-reported openness to psychosocial services and barriers to using those services. Our analyses identified significant relationships between adult attachment dimensions, distress, perceived social support, and openness to and use of psychosocial services. Distress was significantly associated with openness to and use of psychiatric medication. Perceived social support demonstrated significant mediation effects between attachment anxiety and distress. Similarly, perceived social support demonstrated significant mediation effects in the relationship of elevated depression and high attachment avoidance to use of psychiatric medication. However, significant study limitations may be assumed to have negatively impacted the ability to draw meaningful conclusions from the data. Future research would benefit from further examination of the relationships among adult attachment, distress, perceived social support, and openness to and use of psychosocial services. Clearer understanding the nature of these relationships could guide care providers in being able to more effectively provide services to women who are experiencing significant distress but fail to access services. More effective provision of services and subsequent reduction in distress would likely improve health outcomes.
Advisors/Committee Members: Evans, Harry M..
Subjects/Keywords: Social Support; Neoplasms; Stress, Psychological
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APA (6th Edition):
Adams, C. L. (2012). Attachment Influences within a Gynecologic Cancer Population. (Thesis). University of Texas Southwestern Medical Center. Retrieved from http://hdl.handle.net/2152.5/1212
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Adams, Cassandra Leigh. “Attachment Influences within a Gynecologic Cancer Population.” 2012. Thesis, University of Texas Southwestern Medical Center. Accessed March 01, 2021.
http://hdl.handle.net/2152.5/1212.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Adams, Cassandra Leigh. “Attachment Influences within a Gynecologic Cancer Population.” 2012. Web. 01 Mar 2021.
Vancouver:
Adams CL. Attachment Influences within a Gynecologic Cancer Population. [Internet] [Thesis]. University of Texas Southwestern Medical Center; 2012. [cited 2021 Mar 01].
Available from: http://hdl.handle.net/2152.5/1212.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Adams CL. Attachment Influences within a Gynecologic Cancer Population. [Thesis]. University of Texas Southwestern Medical Center; 2012. Available from: http://hdl.handle.net/2152.5/1212
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
University of Texas Southwestern Medical Center
14.
Stafford, Jason Hugh.
Targeting Aminophospholipids Exposed on Tumor Endothelium for Tumor Imaging.
Degree: 2012, University of Texas Southwestern Medical Center
URL: http://hdl.handle.net/2152.5/1038
► Advances in noninvasive imaging of human cancer are crucial to improving diagnosis and therapeutic planning. My project was aimed at developing novel imaging agents that…
(more)
▼ Advances in noninvasive imaging of human cancer are crucial to improving diagnosis and therapeutic planning. My project was aimed at developing novel imaging agents that target the aminophospholipidsphosphatidylserine (PS) and phosphatidylethanolamine (PE). PS and PE arenormally intracellular, but become exposed on the surface of tumor endothelial cells (EC). Anti-tumor therapies promote exposure of PS and PE on tumor EC and the tumor cells as well. Therefore, I tested the hypothesis that 1N11, a PS-binding antibody, and duramycin, a PE-binding peptide, could function as tumor imaging agents.
I labeled the F(ab')₂ fragment of 1N11 with the near-infrared fluophore 800CW for optical imaging and the positron emitting isotope iodine-124 (¹²⁴I) for PET imaging. 800CW-1N11 F(ab')₂ clearly imaged subcutaneous and orthotopic U87 gliomas growing in mice with optimal tumor contrast obtained at 24 h post-injection (p.i.). Uptake of 800CW-1N11 F(ab')₂ was approximately 2-fold higher in irradiated U87 tumors. ¹²⁴I-1N11 F(ab')₂ clearly imaged subcutaneous and orthotopic PC3 prostate carcinomas growing in mice with optimal tumor contrast obtained at 48 hr p.i. Importantly, 800CW- and ¹²⁴I-1N11 F(ab')₂ exhibited low uptake in non-target organs (i.e. liver and kidneys).
Unlike PS, PE had not been established as a specific marker of tumor vasculature in the literature. To demonstrate PE was such a marker, I biotinylated duramycin, characterized its binding properties, and used it to determine the distribution of PE on EC in vitro and in vivo. Exposure of cultured EC to hypoxia, acidity, reactive oxygen species, or irradiation resulted in the formation of membrane blebs that were intensely PE-positive. When biotinylated duramycin was intravenously injected into tumor-bearing mice it preferentially localized to the luminal surface of the vascular endothelium in multiple tumor models. PE-positive vessels were observed in and around hypoxic regions of the tumor. With the exception of intertubular vessels of the kidney, normal vessels remained unstained. I also conjugated duramycin to 800CW and used it for optical imaging of RM-9 and TRAMP prostate carcinomas. These results demonstrate that both 1N11 and duramycin can be used to image a variety of tumors and warrant further study as imaging agents.
Advisors/Committee Members: Thorpe, Philip E..
Subjects/Keywords: Phosphatidylethanolamines; Endothelium, Vascular; Neoplasms
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APA ·
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CSE |
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APA (6th Edition):
Stafford, J. H. (2012). Targeting Aminophospholipids Exposed on Tumor Endothelium for Tumor Imaging. (Thesis). University of Texas Southwestern Medical Center. Retrieved from http://hdl.handle.net/2152.5/1038
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Stafford, Jason Hugh. “Targeting Aminophospholipids Exposed on Tumor Endothelium for Tumor Imaging.” 2012. Thesis, University of Texas Southwestern Medical Center. Accessed March 01, 2021.
http://hdl.handle.net/2152.5/1038.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Stafford, Jason Hugh. “Targeting Aminophospholipids Exposed on Tumor Endothelium for Tumor Imaging.” 2012. Web. 01 Mar 2021.
Vancouver:
Stafford JH. Targeting Aminophospholipids Exposed on Tumor Endothelium for Tumor Imaging. [Internet] [Thesis]. University of Texas Southwestern Medical Center; 2012. [cited 2021 Mar 01].
Available from: http://hdl.handle.net/2152.5/1038.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Stafford JH. Targeting Aminophospholipids Exposed on Tumor Endothelium for Tumor Imaging. [Thesis]. University of Texas Southwestern Medical Center; 2012. Available from: http://hdl.handle.net/2152.5/1038
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
University of Texas Southwestern Medical Center
15.
Holohan, Brody Christopher.
Identifying, Characterizing and Inhibiting the Telomerase Regulatory Network.
Degree: 2015, University of Texas Southwestern Medical Center
URL: http://hdl.handle.net/2152.5/5288
► Telomeres, which are structures that cap the ends of linear chromosomes are maintained by telomerase, a reverse transcriptase. Telomere length limits the self-renewal capacity for…
(more)
▼ Telomeres, which are structures that cap the ends of linear chromosomes are maintained by telomerase, a reverse transcriptase. Telomere length limits the self-renewal capacity for telomerase negative cells, and nearly all tumors circumvent this limitation through telomerase expression; as such, telomerase is an attractive target for cancer therapy. In order to identify new targets for anti-telomerase therapy, I demonstrate that a number of candidate genes are required for telomere maintenance in vitro through shRNA-mediated knockdown and telomere length analysis. Further, I show that Perifosine, a drug identified upstream of a number of the candidates can act as a telomerase inhibitor in a majority of cell lines evaluated in vitro as well as induce shortening of the shortest telomeres in tumors from human patients treated with Perifosine in a phase II clinical trial. Additionally, I identify a trans-generational trend in telomere length at birth in human populations that may bias estimates of telomere shortening rate that has public health implications. Lastly, using data from a large twin study, I have identified a network of genes that regulate the rate of telomere shortening in humans that may be used to clarify the association between telomere length, aging and age-related disease.
Advisors/Committee Members: Corey, David R., Brekken, Rolf A., Cobb, Melanie H., Shay, Jerry W., Wright, Woodring E..
Subjects/Keywords: Enzyme Inhibitors; Neoplasms; Phosphorylcholine; Telomerase
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APA ·
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MLA ·
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APA (6th Edition):
Holohan, B. C. (2015). Identifying, Characterizing and Inhibiting the Telomerase Regulatory Network. (Thesis). University of Texas Southwestern Medical Center. Retrieved from http://hdl.handle.net/2152.5/5288
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Holohan, Brody Christopher. “Identifying, Characterizing and Inhibiting the Telomerase Regulatory Network.” 2015. Thesis, University of Texas Southwestern Medical Center. Accessed March 01, 2021.
http://hdl.handle.net/2152.5/5288.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Holohan, Brody Christopher. “Identifying, Characterizing and Inhibiting the Telomerase Regulatory Network.” 2015. Web. 01 Mar 2021.
Vancouver:
Holohan BC. Identifying, Characterizing and Inhibiting the Telomerase Regulatory Network. [Internet] [Thesis]. University of Texas Southwestern Medical Center; 2015. [cited 2021 Mar 01].
Available from: http://hdl.handle.net/2152.5/5288.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Holohan BC. Identifying, Characterizing and Inhibiting the Telomerase Regulatory Network. [Thesis]. University of Texas Southwestern Medical Center; 2015. Available from: http://hdl.handle.net/2152.5/5288
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
University of Texas Southwestern Medical Center
16.
Farley, Demetra Dannielle.
Role for NIP45 in Telomere Recruitment to PML Bodies in ALT Cancer Cells.
Degree: 2013, University of Texas Southwestern Medical Center
URL: http://hdl.handle.net/2152.5/1348
► Telomere length maintenance is critical for continued cell proliferation. The SMC5/6 complex, required for double-strand break (DSB) repair in both yeast and humans, has been…
(more)
▼ Telomere length maintenance is critical for continued cell proliferation. The SMC5/6 complex, required for double-strand break (DSB) repair in both yeast and humans, has been implicated in the maintenance of telomere length in certain cancer cells. In the absence of active telomerase, SMC5/6 complex-dependent homologous recombination is utilized to maintain telomere length at PML bodies, a mechanism referred to as alternative lengthening of telomeres (ALT). Sumoylation of several telomere-binding proteins is required for the localization of telomeres to PML bodies in G2 phase cells (APBs).
We demonstrate that NIP45, a SUMO-like domain (SLD) containing protein, also affects telomere targeting in ALT cells. Loss of endogenous NIP45 protein results in decreased localization of telomeres to PML bodies in a manner independent of the SMC5/6 complex. NIP45 stimulates telomere binding protein sumoylation, as knockdown of the NIP45 protein negatively affects their sumoylation. Importantly, the NIP45 C-terminal SUMO-like domain (SLD2) is sufficient to rescue both APB formation and telomere-binding-protein sumoylation. NIP45 localizes to PML bodies, but not telomeres, in log phase cells, yet interacts efficiently with TIN2, a sumoylatable telomere binding protein. Additionally, a fragment of NIP45 containing the functional SLD2 domain is sufficient to maintain TIN2 binding. We predict, then, that NIP45 might act to recruit telomeres to the PML bodies via its interaction with TIN2, ultimately allowing for SMC5/6 complex-dependent telomere maintenance in G2 phase cells. In keeping with this hypothesis, loss of endogenous TIN2 protein also negatively affects localization of telomeres to PML bodies, even in the presence of NIP45, supporting a requirement for the TIN2-NIP45 interaction in telomere localization to PML bodies.
Through this work, we have defined a role for the NIP45 protein in ALT cancer cell telomere length maintenance, further detailing the mechanism by which telomerase-negative cancer subtypes achieve unlimited replicative potential.
Advisors/Committee Members: Cobb, Melanie H., Yu, Hongtao, Corey, David R., Scaglioni, Pier Paolo.
Subjects/Keywords: Telomere Homeostasis; Carrier Proteins; Neoplasms
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APA (6th Edition):
Farley, D. D. (2013). Role for NIP45 in Telomere Recruitment to PML Bodies in ALT Cancer Cells. (Thesis). University of Texas Southwestern Medical Center. Retrieved from http://hdl.handle.net/2152.5/1348
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Farley, Demetra Dannielle. “Role for NIP45 in Telomere Recruitment to PML Bodies in ALT Cancer Cells.” 2013. Thesis, University of Texas Southwestern Medical Center. Accessed March 01, 2021.
http://hdl.handle.net/2152.5/1348.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Farley, Demetra Dannielle. “Role for NIP45 in Telomere Recruitment to PML Bodies in ALT Cancer Cells.” 2013. Web. 01 Mar 2021.
Vancouver:
Farley DD. Role for NIP45 in Telomere Recruitment to PML Bodies in ALT Cancer Cells. [Internet] [Thesis]. University of Texas Southwestern Medical Center; 2013. [cited 2021 Mar 01].
Available from: http://hdl.handle.net/2152.5/1348.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Farley DD. Role for NIP45 in Telomere Recruitment to PML Bodies in ALT Cancer Cells. [Thesis]. University of Texas Southwestern Medical Center; 2013. Available from: http://hdl.handle.net/2152.5/1348
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
University of Texas Southwestern Medical Center
17.
Patrick, David M.
Regulation of Cellular Growth and Differentiation by MicroRNAs -21 and -451.
Degree: 2013, University of Texas Southwestern Medical Center
URL: http://hdl.handle.net/2152.5/1705
► MicroRNAs are small RNAs approximately 20-24 nucleotides in length that are conserved throughout evolution. MicroRNA genes are transcribed by RNA polymerase II and are processed…
(more)
▼ MicroRNAs are small RNAs approximately 20-24 nucleotides in length that are conserved throughout evolution. MicroRNA genes are transcribed by RNA polymerase II and are processed both in the nucleus and the cytoplasm from longer precursor RNAs. Functionally, microRNAs interact with Argonaute proteins and guide the formation of a complex with messenger RNAs by Watson-Crick base-pair formation between the microRNA and mRNA. This association stimulates the formation of the microRNA-RNA-induced silencing complex which, upon association with essential adaptor molecules such as GW182, recruits transcriptional repressors and mRNA destabilizers. Essential developmental processes such as embryonic stem cell differentiation and cardiovascular development have been shown to be dependent upon microRNAs. MicroRNAs also participate in a variety of disease processes including tumorigenesis and cardiovascular disease.
MicroRNA-451 (miR-451) is regulated during erythrocyte terminal differentiation. The expression of miR-451 is restricted to late erythrocyte precursors and terminally differentiated erythrocytes. We therefore hypothesized that miR-451 plays a role in terminal erythroid differentiation. Deletion of miR-451 in mice results in a terminal erythroid differentiation defect both embryonically and in adulthood. These animals display a reduction in hematocrit and an inability to sustain a high erythropoietic rate. Transient inhibition of miR-451 results in the same defect. Transcript profiling of miR-451-/- erythroblasts revealed upregulation of 14-3-3ξ, a molecule implicated in the regulation of hematopoiesis. Knockdown of 14-3-3ξ with shRNA in miR-451-/- erythroblasts attenuates the differentiation defect. These data show the essential role of miR-451 repression of 14-3-3ξ during terminal erythrocyte differentiation. Finally, the potent effect of miR-451 inhibition on erythrocyte production suggests that this strategy may be efficacious for the treatment of polycythemia vera, a myeloproliferative neoplasm characterzed by excessive erythrocyte production. Inhibition of miR-451 in a mouse model of PV significantly reduces disease burden.
MicroRNA-21 (miR-21) is regulated in a variety of both human and mouse models of disease. MiR-21 has been widely reported as a driver of tumorigenesis and is consistently upregulated in cardiac remodeling. It has been suggested that miR-21 plays a protective role during cardiac hypertrophy, however, an opposing report suggests that miR-21 inhibition is beneficial in a mouse model of cardiac remodeling. We therefore hypothesized that miR-21 played an essential role in cardiac hypertrophy and remodeling. Deletion of miR-21 in mice resulted in no observable phenotype. MiR-21-/- displayed cardiac remodeling, cardiac stress-responsive gene activation, and reduction in cardiac function in response to four cardiac stress models: thoracic aortic constriction, angiotensin II infusion, calcineurin overexpression, and myocardial infarction. Moreover, inhibition of miR-21 with an LNA-modified miR-21…
Advisors/Committee Members: Olson, Eric N., MacDonald, Raymond J., Hill, Joseph A., Johnson, Jane E., Huang, Lily.
Subjects/Keywords: Lung Neoplasms; MicroRNAs; Polycythemia Vera
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APA (6th Edition):
Patrick, D. M. (2013). Regulation of Cellular Growth and Differentiation by MicroRNAs -21 and -451. (Thesis). University of Texas Southwestern Medical Center. Retrieved from http://hdl.handle.net/2152.5/1705
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Patrick, David M. “Regulation of Cellular Growth and Differentiation by MicroRNAs -21 and -451.” 2013. Thesis, University of Texas Southwestern Medical Center. Accessed March 01, 2021.
http://hdl.handle.net/2152.5/1705.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Patrick, David M. “Regulation of Cellular Growth and Differentiation by MicroRNAs -21 and -451.” 2013. Web. 01 Mar 2021.
Vancouver:
Patrick DM. Regulation of Cellular Growth and Differentiation by MicroRNAs -21 and -451. [Internet] [Thesis]. University of Texas Southwestern Medical Center; 2013. [cited 2021 Mar 01].
Available from: http://hdl.handle.net/2152.5/1705.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Patrick DM. Regulation of Cellular Growth and Differentiation by MicroRNAs -21 and -451. [Thesis]. University of Texas Southwestern Medical Center; 2013. Available from: http://hdl.handle.net/2152.5/1705
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
University of Texas Southwestern Medical Center
18.
Cook, Ellen Claire.
The Impact of Specialized Family Camps on Quality of Life and Hope in Families Who Are Coping with Pediatric Cancer.
Degree: 2012, University of Texas Southwestern Medical Center
URL: http://hdl.handle.net/2152.5/1120
► BACKGROUND: Over the past several decades, specialized summer camps for children with cancer have been shown to have various positive results in those who attend.…
(more)
▼ BACKGROUND: Over the past several decades, specialized summer camps for children with cancer have been shown to have various positive results in those who attend. Family camps have become increasingly popular over the past few years, but the efficacy of family camps for specialized populations has not been well established through research. In addition, few studies have addressed the benefits of the camp experience over time, especially in regard to its impact on quality of life. The aim of this study was to learn whether or not the family camp experience increases the quality of life of families with a child with cancer, and whether or not these changes are maintained after the camp experience ends. In addition, this study examined the impact of camp on levels of hope, and analyzed how hope and social support contribute to the quality of life of those who attend camp.
SUBJECTS: A total of 66 families participated in this study. Participants include parents, cancer patients or survivors, and siblings. Thirty-nine families who attended a specialized weekend camp participated in the study, and a control sample of 27 families who did not attend camp was recruited as well.
METHOD: Questionnaire data was collected at three time points: pre-camp, post-camp, and a three-month follow up. Measures included a demographic questionnaire, age appropriate versions of the PedsQLTM 4.0 Generic Core Scales, the PedsQLTM 2.0 PedsQLTM 2.0 Family Impact Module, the Hope Scale (Adult and Child versions), the Young Children’s Hope Scale, and a brief follow-up questionnaire.
RESULTS: Quality of life did not significantly increase in the camp group in the overall family unit. However, quality of life was shown to be significantly higher in the camp group than the control group at the beginning and end of camp. Siblings demonstrated a significant increase in quality of life when examined separately from the family unit. No significant changes in hope or differences in hope between groups were observed.
DISCUSSION: Though this study did not demonstrate the efficacy of family camp as predicted, it did show that individual family members are impacted by camp in different ways. Camp has been show to benefit siblings in particular, which is indicated by improvement in quality of life, hope, and social support in this population. This study also shows that different results may be found using different measures of the same variables.
Advisors/Committee Members: Wetherington, Crista E., Germann, Julie, Chiu, Chung-Yi, Leavey, Patrick.
Subjects/Keywords: Family; Neoplasms; Quality of Life
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APA (6th Edition):
Cook, E. C. (2012). The Impact of Specialized Family Camps on Quality of Life and Hope in Families Who Are Coping with Pediatric Cancer. (Thesis). University of Texas Southwestern Medical Center. Retrieved from http://hdl.handle.net/2152.5/1120
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Cook, Ellen Claire. “The Impact of Specialized Family Camps on Quality of Life and Hope in Families Who Are Coping with Pediatric Cancer.” 2012. Thesis, University of Texas Southwestern Medical Center. Accessed March 01, 2021.
http://hdl.handle.net/2152.5/1120.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Cook, Ellen Claire. “The Impact of Specialized Family Camps on Quality of Life and Hope in Families Who Are Coping with Pediatric Cancer.” 2012. Web. 01 Mar 2021.
Vancouver:
Cook EC. The Impact of Specialized Family Camps on Quality of Life and Hope in Families Who Are Coping with Pediatric Cancer. [Internet] [Thesis]. University of Texas Southwestern Medical Center; 2012. [cited 2021 Mar 01].
Available from: http://hdl.handle.net/2152.5/1120.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Cook EC. The Impact of Specialized Family Camps on Quality of Life and Hope in Families Who Are Coping with Pediatric Cancer. [Thesis]. University of Texas Southwestern Medical Center; 2012. Available from: http://hdl.handle.net/2152.5/1120
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
University of Texas Southwestern Medical Center
19.
Rivera, Lee Benjamin.
Regulation of Pericyte Behavior by Secreted Protein Acidic and Rich in Cysteine.
Degree: 2011, University of Texas Southwestern Medical Center
URL: http://hdl.handle.net/2152.5/863
► Pericytes migrate to newly formed vessels where they induce vessel quiescence and promote vessel stability. Secreted Protein Acidic and Rich in Cysteine (SPARC) is a…
(more)
▼ Pericytes migrate to newly formed vessels where they induce vessel quiescence and promote vessel stability. Secreted Protein Acidic and Rich in Cysteine (SPARC) is a multifunctional matricellular protein believed to be involved in the regulation of vascular cell migration and proliferation during angiogenesis. We previously found that SPARC-deficient mice exhibited abnormal vascular function and decreased pericyte-associated vessels in an orthotopic model of pancreatic ductal adenocarcinoma (PDAC), suggesting that SPARC regulates pericyte behavior during tumor angiogenesis.
Pericyte expression of SPARC was detected in PDAC lesions of P48Cre: LSLKrasG12D: Ink4Alox/lox mice as well as in normal mouse pancreata by indirect immunofluorescence. Primary mouse pericytes were isolated and the following parameters were characterized: proliferation, migration, the ability to induce bEnd.3 cell cord formation, TGFbeta1-induced activity, and TGFbeta1 receptor expression.
Here I report that SPARC regulates pericyte migration. I found that SPARC is expressed by pericytes in vivo and confirmed that SPARC-deficient mice have fewer pericyte-associated vessels using a transgenic model of PDAC. Primary pericytes isolated from SPARC-/- mice proliferate faster than their SPARC+/+ counterparts but are less able to induce bEnd.3 cord-formation in vitro. SPARC deficiency also results in defective filopodia and focal adhesion formation and impedes pericyte migration, an effect that is blocked by inhibiting TGFbeta. Furthermore, I demonstrate that SPARC interacts with the TGFbeta1 accessory receptor endoglin in pericytes. In SPARC-deficient pericytes, endoglin aberrantly associates with focal complexes. SPARC deficiency also induces endoglin-mediated, TGFbeta1-induced blockade of pericyte migration, and results in alphaV integrin-mediated activation of TGFbeta1.
These results demonstrate that SPARC controls pericyte migration by regulating endoglin and alphaV integrin-mediated TGFbeta1 activity.
Advisors/Committee Members: Brekken, Rolf A..
Subjects/Keywords: Pancreatic Neoplasms; Neovascularization, Pathologic; Osteonectin
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APA (6th Edition):
Rivera, L. B. (2011). Regulation of Pericyte Behavior by Secreted Protein Acidic and Rich in Cysteine. (Thesis). University of Texas Southwestern Medical Center. Retrieved from http://hdl.handle.net/2152.5/863
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Rivera, Lee Benjamin. “Regulation of Pericyte Behavior by Secreted Protein Acidic and Rich in Cysteine.” 2011. Thesis, University of Texas Southwestern Medical Center. Accessed March 01, 2021.
http://hdl.handle.net/2152.5/863.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Rivera, Lee Benjamin. “Regulation of Pericyte Behavior by Secreted Protein Acidic and Rich in Cysteine.” 2011. Web. 01 Mar 2021.
Vancouver:
Rivera LB. Regulation of Pericyte Behavior by Secreted Protein Acidic and Rich in Cysteine. [Internet] [Thesis]. University of Texas Southwestern Medical Center; 2011. [cited 2021 Mar 01].
Available from: http://hdl.handle.net/2152.5/863.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Rivera LB. Regulation of Pericyte Behavior by Secreted Protein Acidic and Rich in Cysteine. [Thesis]. University of Texas Southwestern Medical Center; 2011. Available from: http://hdl.handle.net/2152.5/863
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
University of Texas Southwestern Medical Center
20.
Diaz, Tracy Montie.
Development of Novel Cancer Immunotherapeutics Utilizing Cell-Targeting Peptides.
Degree: 2013, University of Texas Southwestern Medical Center
URL: http://hdl.handle.net/2152.5/1242
► Cancer immunotherapy is an emerging treatment option that offers high tumor specificity and efficacy. Immune therapies for cancer can be divided into two main types:…
(more)
▼ Cancer immunotherapy is an emerging treatment option that offers high tumor specificity and efficacy. Immune therapies for cancer can be divided into two main types: active and passive. Active therapy strives to achieve a long term protective immunity against a tumor antigen while passive therapy supplies exogenous immunological reagents for anti-tumor effector functions. Both immunotherapies can be improved by utilizing cell targeting peptides.
Dendritic Cell Targeting Peptides: Cancer vaccines can elicit immune responses against tumor antigens. Antigen-pulsed in vitro matured dendritic cells (DCs) are used for higher efficacy. However, this method does not provide a significant therapeutic immune response. A more robust anti-tumor immune response could potentially be achieved through in vivo DC targeting of tumor antigens. Through phage-displayed peptide library panning protocol, four different DC-targeting phage clones were isolated. Of those, XS52.1 and XS52.3 bind specifically to the XS52 immature dendritic cells. The XS52.3 phage clone also binds bone-marrow dendritic cells (BMDCs) from Balb/c and C57BL/J6 mice. Each phage clone elicited heightened anti-phage antibody production in both mouse strains. Potential future studies will determine if these peptides can be used to target antigen to DCs for in vivo cancer vaccines.
Peptide-Antibody Targeting: Monoclonal antibodies directed against tumor antigens have been successful in clinics, but problems remain with identifying and validating new targets. Modification of the antibody scaffold for distinct applications can also be problematic. Using our phage display panning protocol, we have identified ligands of high affinity and specificity against a panel of human non-small cell lung cancer (NSCLC) cell lines. Furthermore, these peptide-targeting ligands can be chemically synthesized and easily modified for different uses. In my studies, synthetic-peptide ligands have been used to redirect antibody targeting by using biotinylated-tetrameric peptides and anti-biotin antibodies. These results suggest that peptide-antibody conjugates utilizing isolated peptides can be used to redirect antibody targeting. This methodology would increase the antibody repertoire available for therapy.
Advisors/Committee Members: Brown, Kathlynn C..
Subjects/Keywords: Neoplasms; Peptides; Peptide Library
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APA (6th Edition):
Diaz, T. M. (2013). Development of Novel Cancer Immunotherapeutics Utilizing Cell-Targeting Peptides. (Thesis). University of Texas Southwestern Medical Center. Retrieved from http://hdl.handle.net/2152.5/1242
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Diaz, Tracy Montie. “Development of Novel Cancer Immunotherapeutics Utilizing Cell-Targeting Peptides.” 2013. Thesis, University of Texas Southwestern Medical Center. Accessed March 01, 2021.
http://hdl.handle.net/2152.5/1242.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Diaz, Tracy Montie. “Development of Novel Cancer Immunotherapeutics Utilizing Cell-Targeting Peptides.” 2013. Web. 01 Mar 2021.
Vancouver:
Diaz TM. Development of Novel Cancer Immunotherapeutics Utilizing Cell-Targeting Peptides. [Internet] [Thesis]. University of Texas Southwestern Medical Center; 2013. [cited 2021 Mar 01].
Available from: http://hdl.handle.net/2152.5/1242.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Diaz TM. Development of Novel Cancer Immunotherapeutics Utilizing Cell-Targeting Peptides. [Thesis]. University of Texas Southwestern Medical Center; 2013. Available from: http://hdl.handle.net/2152.5/1242
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
University of Texas Southwestern Medical Center
21.
Thomas, Anna Johnson.
Engagement in Care among Patients with Lung Cancer.
Degree: 2016, University of Texas Southwestern Medical Center
URL: http://hdl.handle.net/2152.5/5746
► BACKGROUND: Engagement in care broadly refers to patients' knowledge, skills, ability and willingness to play an active role in their health. Patient activation is a…
(more)
▼ BACKGROUND: Engagement in care broadly refers to patients' knowledge, skills, ability and willingness to play an active role in their health. Patient activation is a core component of engagement and has been associated with better care outcomes in several chronic health populations. Despite these findings, patient activation has been understudied in oncology populations broadly, with little focus on lung cancer patients specifically.
OBJECTIVE: The primary aim of this study was to describe patient activation among surveyed lung cancer patients. A second aim was to explore specific demographic, smoking-related, and psychosocial correlates of patient activation.
METHOD: The sample included 231 lung patients who participated in a cross-sectional, multisite study. Patients completed a patient activation self-report instrument, along with demographic, clinical, and psychosocial measures.
ANALYSIS: Data related to study aims were analyzed using univariate and multivariable analysis.
RESULTS: Among lung cancer patients, overall patient activation scores were significantly lower for current smokers compared to former and never smokers (F (2, 218) = 4.50, p = 0.01), for men when compared with women (F (1, 220) = 8.75, p < 0.01), and for those who reported clinically significant depressive symptoms when compared with those who did not (F (1, 219) = 13.95, p < 0.001). In multivariable analysis, these significant main effects of smoking status, gender, and depressive symptoms remained.
DISCUSSION: Among lung cancer patients, those who were current smokers, were male, and those who endorsed high depressive symptomatology were likely to have the lowest activation scores in the sample. Practical recommendations include clinically identifying these high-risk patients through assessment and addressing activation via evidence based intervention.
Advisors/Committee Members: Hamann, Heidi, Kendall, Jeffrey, Germann, Julie, Howe-Martin, Laura, Whitfill, Travis.
Subjects/Keywords: Depression; Lung Neoplasms; Patient Participation
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APA (6th Edition):
Thomas, A. J. (2016). Engagement in Care among Patients with Lung Cancer. (Thesis). University of Texas Southwestern Medical Center. Retrieved from http://hdl.handle.net/2152.5/5746
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Thomas, Anna Johnson. “Engagement in Care among Patients with Lung Cancer.” 2016. Thesis, University of Texas Southwestern Medical Center. Accessed March 01, 2021.
http://hdl.handle.net/2152.5/5746.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Thomas, Anna Johnson. “Engagement in Care among Patients with Lung Cancer.” 2016. Web. 01 Mar 2021.
Vancouver:
Thomas AJ. Engagement in Care among Patients with Lung Cancer. [Internet] [Thesis]. University of Texas Southwestern Medical Center; 2016. [cited 2021 Mar 01].
Available from: http://hdl.handle.net/2152.5/5746.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Thomas AJ. Engagement in Care among Patients with Lung Cancer. [Thesis]. University of Texas Southwestern Medical Center; 2016. Available from: http://hdl.handle.net/2152.5/5746
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
University of Nairobi
22.
Ahomo, E. J. O.
Fine needle aspiration biopsy compared to surgical open biosy in management of bone neoplasms
.
Degree: 2004, University of Nairobi
URL: http://hdl.handle.net/11295/6204
► This was a prospective study to evaluate FNAB/C on bone neoplasms. It was designed to determine the reliability, area of diagnostic difficulty and limitations of…
(more)
▼ This was a prospective study to evaluate FNAB/C on bone neoplasms. It was designed to determine the reliability, area of diagnostic difficulty and limitations of FNAB/C on bone neoplasms encountered at KNH. A total of 59 patients were recruited between 1 st OctobeF2003 and 30th August 2004. Patients who had previous histopathological diagnosis by open biopsy were excluded. Aspirate material from 53 of the 59 patients were considered adequate for cytological diagnosis. There were 6 insufficient aspirates. Of these 53, 44 aspirates were conclusive for specific diagnosis of which 9 were sufficient but undiagnostic. The aspirates were categorized into 5 groups; primary benign bone tumuors 13, primary malignant bone tumuours 11, metastases 5, soft tissue tumours 3 and osteomyelitis 12. Cytohistopathologic correlation was possible in 51 aspirates. There were 36 true positives and this gave an overall accuracy for FNAB/C of 70.6% and 92.3% excluding the inconclusive aspirates. The specificity and sensitivity of FNAB/C was 27.2% and 75% respectively. The positive predictive value and negative predictive value was 81.8% and 20% respectively. The mean time lapse between first clinic visit and procedure for FNAB/C and open biopsy was 5 and 26 days and for first clinic visit and diagnostic confirmation 19 and 50 days respectively. Complications occurred more in open biopsy with overall of 61 % compared to 18.6% of FNAB/C. Average costs for procedures were 100 shillings for FNAB/C and 5,056 shillings for open biopsy. This study demonstrates that FNAB/C is reliable, quick and acceptably accurate procedure. Not only is it convenient but its well tolerated with low morbidity and is cost effective. However, it cannot entirely replace open biopsy. Future efforts should focus on teamwork, more training for clinicians, pathologists and radiologists on this procedure to improve skill and improved instrumentation to obtain higher rate of conclusive aspirate material.
Subjects/Keywords: Bones;
neoplasms
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APA ·
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APA (6th Edition):
Ahomo, E. J. O. (2004). Fine needle aspiration biopsy compared to surgical open biosy in management of bone neoplasms
. (Thesis). University of Nairobi. Retrieved from http://hdl.handle.net/11295/6204
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Ahomo, E J O. “Fine needle aspiration biopsy compared to surgical open biosy in management of bone neoplasms
.” 2004. Thesis, University of Nairobi. Accessed March 01, 2021.
http://hdl.handle.net/11295/6204.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Ahomo, E J O. “Fine needle aspiration biopsy compared to surgical open biosy in management of bone neoplasms
.” 2004. Web. 01 Mar 2021.
Vancouver:
Ahomo EJO. Fine needle aspiration biopsy compared to surgical open biosy in management of bone neoplasms
. [Internet] [Thesis]. University of Nairobi; 2004. [cited 2021 Mar 01].
Available from: http://hdl.handle.net/11295/6204.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Ahomo EJO. Fine needle aspiration biopsy compared to surgical open biosy in management of bone neoplasms
. [Thesis]. University of Nairobi; 2004. Available from: http://hdl.handle.net/11295/6204
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
University of Texas Southwestern Medical Center
23.
Croom, Andrea Rose.
Illness Perceptions of Patients with Late-Stage Cancer and Their Partners.
Degree: 2013, University of Texas Southwestern Medical Center
URL: http://hdl.handle.net/2152.5/1237
► As treatments improve and patients with late-stage cancer live longer, it is important to examine factors associated with their psychological adjustment and illness-related behaviors. The…
(more)
▼ As treatments improve and patients with late-stage cancer live longer, it is important to examine factors associated with their psychological adjustment and illness-related behaviors. The current studies used the Common-sense Model of Self-regulation (Leventhal, Brissette, & Leventhal, 2003) to understand how patients and their partners manage the demands of this understudied illness context. The Common-sense Model proposes that individuals create mental representations about their illness to make sense of and develop strategies to manage the illness. The current studies were the first to examine (a) whether patients’ illness perceptions are associated with advanced illness behaviors (e.g., completing advance directives; NCI, 2005), which are crucial for receiving quality care at the end-of-life, and (b) whether perceptions of both patients and partners are associated with psychological adjustment. A dyadic perspective is important because cancer is not an individual experience, but rather affects significant others in the patient’s life.
Female patients with late-stage breast, gynecological, or lung cancer and their spouses or unmarried partners independently completed self-report measures of their illness perceptions, relationship experience, psychological adjustment, and advanced illness behaviors (N= 105 patients and 88 partners). Data were examined at both intrapersonal (individual) and interpersonal (dyadic) levels. The first study demonstrated that patients’ individual perceptions of cancer were better predictors of their quality of life than clinical characteristics of cancer (e.g., stage, illness duration). Advanced illness behaviors were associated with higher quality of life and were predicted by illness perceptions (i.e., illness severity and illness coherence), as well as by clinical and personal characteristics. The second study suggested that patients’ and partners’ psychological adjustment reflected their individual illness perceptions, as well as aspects of their relationship (i.e., relationship quality and social constraints in discussing cancer). There was limited evidence that incongruence in patients’ and partners’ illness perceptions was related to poorer adjustment, potentially because illness perception congruence was high. Findings from these studies demonstrate the importance of designing interventions to improve communication between patients, partners, and health care professionals about late-stage cancer beliefs.
Advisors/Committee Members: Wiebe, Deborah J..
Subjects/Keywords: Neoplasms; Catastrophic Illness; Spouses
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APA ·
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MLA ·
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CSE |
Export
to Zotero / EndNote / Reference
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APA (6th Edition):
Croom, A. R. (2013). Illness Perceptions of Patients with Late-Stage Cancer and Their Partners. (Thesis). University of Texas Southwestern Medical Center. Retrieved from http://hdl.handle.net/2152.5/1237
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Croom, Andrea Rose. “Illness Perceptions of Patients with Late-Stage Cancer and Their Partners.” 2013. Thesis, University of Texas Southwestern Medical Center. Accessed March 01, 2021.
http://hdl.handle.net/2152.5/1237.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Croom, Andrea Rose. “Illness Perceptions of Patients with Late-Stage Cancer and Their Partners.” 2013. Web. 01 Mar 2021.
Vancouver:
Croom AR. Illness Perceptions of Patients with Late-Stage Cancer and Their Partners. [Internet] [Thesis]. University of Texas Southwestern Medical Center; 2013. [cited 2021 Mar 01].
Available from: http://hdl.handle.net/2152.5/1237.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Croom AR. Illness Perceptions of Patients with Late-Stage Cancer and Their Partners. [Thesis]. University of Texas Southwestern Medical Center; 2013. Available from: http://hdl.handle.net/2152.5/1237
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
University of Texas Southwestern Medical Center
24.
Tarkenton, Tahnae R'shelle.
Peer Victimization in the Pediatric Oncology Population: Review of Risks, Protective Factors, and Implications for Intervention.
Degree: 2015, University of Texas Southwestern Medical Center
URL: http://hdl.handle.net/2152.5/1720
► Childhood cancer presents patients and their families with unique short- and long-term challenges that can disrupt physical, emotional, academic, and family/social functioning. Further, many psychosocial…
(more)
▼ Childhood cancer presents patients and their families with unique short- and long-term challenges that can disrupt physical, emotional, academic, and family/social functioning. Further, many psychosocial adjustment difficulties common in the pediatric oncology population are similar to those that place healthy children at risk for peer victimization. Thus, pediatric oncology patients may be at increased risk for peer victimization. Based on current literature, this document will address the following hypotheses: (1.) children and adolescents with cancer are more at risk for peer victimization than healthy youth, (2.) children and adolescents with cancer are more susceptible to negative effects of peer victimization than healthy youth, and (3.) research examining anti-bullying interventions will indicate effective strategies that can be tailored to reduce peer victimization’s prevalence, effects, and risks in the pediatric oncology population. To address the hypotheses, potential risks, protective factors, and adverse outcomes linking peer victimization to the pediatric cancer population will be reviewed. Then, existing intervention strategies shown to be effective in preventing and reducing effects of peer victimization in healthy populations will be presented. Lastly, a manualized peer victimization intervention program tailored to the pediatric oncology population will be provided.
Advisors/Committee Members: Wetherington, Crista E., Roop, Melissa, Heppner, Celia, Germann, Julie.
Subjects/Keywords: Adolescent; Bullying; Neoplasms; Peer Group
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APA (6th Edition):
Tarkenton, T. R. (2015). Peer Victimization in the Pediatric Oncology Population: Review of Risks, Protective Factors, and Implications for Intervention. (Thesis). University of Texas Southwestern Medical Center. Retrieved from http://hdl.handle.net/2152.5/1720
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Tarkenton, Tahnae R'shelle. “Peer Victimization in the Pediatric Oncology Population: Review of Risks, Protective Factors, and Implications for Intervention.” 2015. Thesis, University of Texas Southwestern Medical Center. Accessed March 01, 2021.
http://hdl.handle.net/2152.5/1720.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Tarkenton, Tahnae R'shelle. “Peer Victimization in the Pediatric Oncology Population: Review of Risks, Protective Factors, and Implications for Intervention.” 2015. Web. 01 Mar 2021.
Vancouver:
Tarkenton TR. Peer Victimization in the Pediatric Oncology Population: Review of Risks, Protective Factors, and Implications for Intervention. [Internet] [Thesis]. University of Texas Southwestern Medical Center; 2015. [cited 2021 Mar 01].
Available from: http://hdl.handle.net/2152.5/1720.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Tarkenton TR. Peer Victimization in the Pediatric Oncology Population: Review of Risks, Protective Factors, and Implications for Intervention. [Thesis]. University of Texas Southwestern Medical Center; 2015. Available from: http://hdl.handle.net/2152.5/1720
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
University of Texas Southwestern Medical Center
25.
Velmurugan, Ramraj.
Using Advanced Microscopy Techniques for the Study of Macrophage-Cancer Cell Interactions in the Presence of Therapeutic Antibodies.
Degree: 2017, University of Texas Southwestern Medical Center
URL: http://hdl.handle.net/2152.5/7195
► The file named "VELMURUGAN-DISSERTATION-2017.pdf" is the primary dissertation file. Eight (8) supplemental files are also available and may be viewed individually.
The use of monoclonal…
(more)
▼ The file named "VELMURUGAN-DISSERTATION-2017.pdf" is the primary dissertation file. Eight (8) supplemental files are also available and may be viewed individually.
The use of monoclonal antibodies represents a rapidly expanding area for cancer therapy. One of the main mechanisms of action of these antibodies is Fcγ receptor-mediated engagement of macrophages and other immune cells. When macrophages engage tumor cells opsonized with antibody molecules, they can perform trogocytosis, the process of internalizing fragments of the target cell, or phagocytosis, the internalization of entire cancer cells. This study first establishes whether the process of trogocytosis can lead to cancer cell death. A variety of microscopy and flow cytometric assays were used to quantify the levels of trogocytosis and cell death, in co-cultures of macrophages and cancer cells. Using HER2-overexpressing breast cancer cell lines and anti-HER2 antibodies, we show that persistent trogocytosis can lead to the killing of cancer cells. The mechanism of trogocytosis was also explored using multifocal plane microscopy (MUM). Imaging the process of trogocytosis using MUM revealed that it proceeds through the macrophage-mediated extrusion of tubular structures of the target cell membrane. This membrane-tubulation results in the preferential uptake of the membrane components from the target cell. The study also investigated the maturation pathway followed by phagosomes containing entire cancer cells. A vacuole-like structure associates with these phagosomes, which whilst also lysosomal in nature, displays characteristics distinct from the phagosome itself. The interface between the vacuole and the phagosome is impermeable to certain solutes as observed through microscopy. Further, the size of the phagosome-associated vacuole is affected by inhibition of the mTOR pathway. Use of advanced microscopy techniques such as MUM in these and other biological problems provides mechanistic insight at the spatiotemporal level. To further develop the algorithms involved in MUM data processing, I have therefore also explored various non-parametric methods of estimating the axial location of point sources from MUM data. A new non-parametric method is proposed, which uses multiple intensities calculated from each image of a point source in MUM data. The performance of this approach is compared with other non-parametric methods through simulations and Fisher information calculations. The effectiveness of this method on experimental data is also evaluated.
Advisors/Committee Members: Li, Wen-Hong, Ward, E. Sally, Ober, Raimund J., Alexandrakis, Georgios, Pasare, Chandrashekhar.
Subjects/Keywords: Antibodies; Breast Neoplasms; Macrophages; Phagocytosis
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APA ·
Chicago ·
MLA ·
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CSE |
Export
to Zotero / EndNote / Reference
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APA (6th Edition):
Velmurugan, R. (2017). Using Advanced Microscopy Techniques for the Study of Macrophage-Cancer Cell Interactions in the Presence of Therapeutic Antibodies. (Thesis). University of Texas Southwestern Medical Center. Retrieved from http://hdl.handle.net/2152.5/7195
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Velmurugan, Ramraj. “Using Advanced Microscopy Techniques for the Study of Macrophage-Cancer Cell Interactions in the Presence of Therapeutic Antibodies.” 2017. Thesis, University of Texas Southwestern Medical Center. Accessed March 01, 2021.
http://hdl.handle.net/2152.5/7195.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Velmurugan, Ramraj. “Using Advanced Microscopy Techniques for the Study of Macrophage-Cancer Cell Interactions in the Presence of Therapeutic Antibodies.” 2017. Web. 01 Mar 2021.
Vancouver:
Velmurugan R. Using Advanced Microscopy Techniques for the Study of Macrophage-Cancer Cell Interactions in the Presence of Therapeutic Antibodies. [Internet] [Thesis]. University of Texas Southwestern Medical Center; 2017. [cited 2021 Mar 01].
Available from: http://hdl.handle.net/2152.5/7195.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Velmurugan R. Using Advanced Microscopy Techniques for the Study of Macrophage-Cancer Cell Interactions in the Presence of Therapeutic Antibodies. [Thesis]. University of Texas Southwestern Medical Center; 2017. Available from: http://hdl.handle.net/2152.5/7195
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
University of Texas Southwestern Medical Center
26.
Prindiville, Katherine Alexis.
Socioeconomic Status and Access to Resources as Predictors of Sibling Hope and Sibling Coping with Pediatric Cancer-Related Stressors.
Degree: 2016, University of Texas Southwestern Medical Center
URL: http://hdl.handle.net/2152.5/3605
► Although most siblings of pediatric cancer patients adjust well to cancer diagnosis and treatment course, some siblings demonstrate significant adjustment difficulties. One question is whether…
(more)
▼ Although most siblings of pediatric cancer patients adjust well to cancer diagnosis and treatment course, some siblings demonstrate significant adjustment difficulties. One question is whether these siblings may also be at risk for reduced hope and poor coping, especially if family roles and routines are particularly disrupted during cancer treatment. This study will examine the degree to which sociodemographic variables (i.e., socioeconomic status and access to resources) predict pediatric cancer patients' siblings' hope and coping. Data were obtained from siblings of pediatric oncology patients and their parents at a large pediatric cancer treatment center using paper-and-pencil questionnaires, telephone/in-person structured interviews, and internet-based questionnaires. I hypothesized a direct relation between sociodemographic variables and sibling hope. I also hypothesized a direct relation between sociodemographic variables and adaptive coping and an inverse relation between sociodemographic variables and maladaptive coping. Both hypotheses were partially supported; sociodemographics as a whole did not significantly predict hope or adaptive coping, but did account for 5% and 10% of the variance, respectively. Sociodemographics did not significantly predict internalizing/externalizing coping or avoidant coping. To rule out superfluous findings, more research on the predictive value of income is needed. Future studies should also further examine other components of socioeconomic status and access to resources on sibling adjustment to pediatric cancer diagnosis and treatment. Life disruption variables significantly predicted all outcome variables, indicating that life disruption plays an important role in sibling adjustment. Providers should be aware of the impact of life disruption and find ways to care for siblings and families to ensure they experience as little disruption as possible.
Advisors/Committee Members: Faith, Melissa A., Germann, Julie, Holland, Alice A., Holm, Suzanne.
Subjects/Keywords: Neoplasms; Parents; Siblings; Stress, Psychological
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APA (6th Edition):
Prindiville, K. A. (2016). Socioeconomic Status and Access to Resources as Predictors of Sibling Hope and Sibling Coping with Pediatric Cancer-Related Stressors. (Thesis). University of Texas Southwestern Medical Center. Retrieved from http://hdl.handle.net/2152.5/3605
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Prindiville, Katherine Alexis. “Socioeconomic Status and Access to Resources as Predictors of Sibling Hope and Sibling Coping with Pediatric Cancer-Related Stressors.” 2016. Thesis, University of Texas Southwestern Medical Center. Accessed March 01, 2021.
http://hdl.handle.net/2152.5/3605.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Prindiville, Katherine Alexis. “Socioeconomic Status and Access to Resources as Predictors of Sibling Hope and Sibling Coping with Pediatric Cancer-Related Stressors.” 2016. Web. 01 Mar 2021.
Vancouver:
Prindiville KA. Socioeconomic Status and Access to Resources as Predictors of Sibling Hope and Sibling Coping with Pediatric Cancer-Related Stressors. [Internet] [Thesis]. University of Texas Southwestern Medical Center; 2016. [cited 2021 Mar 01].
Available from: http://hdl.handle.net/2152.5/3605.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Prindiville KA. Socioeconomic Status and Access to Resources as Predictors of Sibling Hope and Sibling Coping with Pediatric Cancer-Related Stressors. [Thesis]. University of Texas Southwestern Medical Center; 2016. Available from: http://hdl.handle.net/2152.5/3605
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
University of Texas Southwestern Medical Center
27.
Steininger, Robert Joseph, III.
Investigating Roles for Cellular Heterogeneity in Cancer.
Degree: 2014, University of Texas Southwestern Medical Center
URL: http://hdl.handle.net/2152.5/3589
► Cell populations, even those derived from a single clone, can exhibit a high degree of phenotypic variability. However, most biological studies take measurements as averages…
(more)
▼ Cell populations, even those derived from a single clone, can exhibit a high degree of phenotypic variability. However, most biological studies take measurements as averages of entire populations without consideration for the underlying distribution of cellular phenotypes. Though there is growing evidence that variability within cellular populations has some functional consequences, the significance of cell to cell heterogeneity is still poorly understood. Here, we present an analytical platform that represents heterogeneity of cell populations as mixtures of distinct cell phenotypes, or subpopulations, based on immunofluorescent images. These "subpopulation profiles" make the heterogeneity of cell populations more tractable and comparable. We go on to demonstrate that subpopulation profiles can be predictive of clonal populations' drug responses. This separation is shown to be independent of the population's cell-cycle distribution. The subpopulation profiles are then shown to be robust population readouts and used to classify diverse cell lines. We show that, in diverse panels of cell populations, the relationship between basal state heterogeneity and drug response tends to break down. We also show, however, that the subpopulation profiles of diverse cell lines can be useful for identifying independently informative biomarkers. Taken together, these results demonstrate that a subpopulation level reduction of heterogeneity can be a useful readout of cell populations with many potential applications.
Advisors/Committee Members: Pearson, Gray W., Ranganathan, Rama, White, Michael A., Altschuler, Steven J., Wu, Lani.
Subjects/Keywords: Genetic Heterogeneity; Neoplasms; Physiological Processes
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APA (6th Edition):
Steininger, Robert Joseph, I. (2014). Investigating Roles for Cellular Heterogeneity in Cancer. (Thesis). University of Texas Southwestern Medical Center. Retrieved from http://hdl.handle.net/2152.5/3589
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Steininger, Robert Joseph, III. “Investigating Roles for Cellular Heterogeneity in Cancer.” 2014. Thesis, University of Texas Southwestern Medical Center. Accessed March 01, 2021.
http://hdl.handle.net/2152.5/3589.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Steininger, Robert Joseph, III. “Investigating Roles for Cellular Heterogeneity in Cancer.” 2014. Web. 01 Mar 2021.
Vancouver:
Steininger, Robert Joseph I. Investigating Roles for Cellular Heterogeneity in Cancer. [Internet] [Thesis]. University of Texas Southwestern Medical Center; 2014. [cited 2021 Mar 01].
Available from: http://hdl.handle.net/2152.5/3589.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Steininger, Robert Joseph I. Investigating Roles for Cellular Heterogeneity in Cancer. [Thesis]. University of Texas Southwestern Medical Center; 2014. Available from: http://hdl.handle.net/2152.5/3589
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
University of Texas Southwestern Medical Center
28.
Sullivan, Laura Anne.
Anti-Angiogenic Therapy in Non-Small Cell Lung Cancer: Characterizing a New Therapy and Investigating Potential Mechanisms of Resistance.
Degree: 2012, University of Texas Southwestern Medical Center
URL: http://hdl.handle.net/2152.5/1040
► Angiogenesis is the development of blood vessels from a pre-existing vascular network. This process is essential during growth, development and wound healing and plays a…
(more)
▼ Angiogenesis is the development of blood vessels from a pre-existing vascular network. This process is essential during growth, development and wound healing and plays a critical role in the growth and progression of cancer. Initial tumor size is restricted by the diffusion capacity of oxygen and nutrients from surrounding blood vessels. Therefore, to progress beyond a volume of several millimeters, a tumor must stimulate angiogenesis to generate a vascular network that will supply the tumor with the necessary blood, oxygen and nutrients that will allow for continued growth, invasion and metastasis.
Over forty years ago, Judah Folkman hypothesized that targeting tumor angiogenesis would be beneficial for cancer patients. One of the first targets for this new class of drugs was vascular endothelial growth factor (VEGF) a predominant mediator of physiological and pathological angiogenesis. Bevacizumab (Avastin®, Genentech/Roche), a humanized monoclonal antibody that recognizes human VEGF and blocks VEGF from binding to VEGF receptor (VEGFR) 1 and 2, was the first anti-angiogenic drug approved by the United States Food and Drug Administration for the treatment of cancer and remains the gold standard for this class of therapeutics. The Brekken laboratory, in collaborations with Peregrine Pharmaceuticals and Affitech A/S has generated a fully human monoclonal antibody, r84 that recognizes mouse and human VEGF and blocks VEGF binding only to VEGFR2. The data presented in the first half of this dissertation demonstrate the specificity of r84 for VEGF in vitro and in vivo, the efficacy of r84 to control tumor growth and the superior safety profile of r84 as compared to bevacizumab.
Although anti-angiogenic therapy was highly anticipated to have great success in patients, overall results have been somewhat disappointing with modest improvements in patient progression free survival and few improvements to overall survival. In addition, with the expanding use of anti-angiogenic drugs such as bevacizumab and a host of receptor tyrosine kinase inhibitors in the clinic, it is becoming increasingly apparent that not all tumors respond or maintain sensitivity to treatment. Therefore, it is increasingly important to identify mechanisms of resistance to anti-angiogenic therapy so that new drug targets can be identified and/or patients can be appropriately screened for markers that can predict for resistance or sensitivity to anti-angiogenic therapy de novo. Non-small cell lung cancer (NSCLC), the most common form of lung cancer, claims the most new diagnoses and cancer-related deaths than any other cancer worldwide and the therapeutic options currently available for this disease, including bevacizumab have done little to change this statistic. The latter half of this thesis focuses on the in vivo screening of human NSCLC cell lines to identify mechanisms of resistance to the anti-angiogenic monoclonal antibodies bevacizumab and r84 in non-small cell lung cancer.
Advisors/Committee Members: Brekken, Rolf A..
Subjects/Keywords: Antineoplastic Agents; Neoplasms; Antibodies, Monoclonal
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APA ·
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APA (6th Edition):
Sullivan, L. A. (2012). Anti-Angiogenic Therapy in Non-Small Cell Lung Cancer: Characterizing a New Therapy and Investigating Potential Mechanisms of Resistance. (Thesis). University of Texas Southwestern Medical Center. Retrieved from http://hdl.handle.net/2152.5/1040
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Sullivan, Laura Anne. “Anti-Angiogenic Therapy in Non-Small Cell Lung Cancer: Characterizing a New Therapy and Investigating Potential Mechanisms of Resistance.” 2012. Thesis, University of Texas Southwestern Medical Center. Accessed March 01, 2021.
http://hdl.handle.net/2152.5/1040.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Sullivan, Laura Anne. “Anti-Angiogenic Therapy in Non-Small Cell Lung Cancer: Characterizing a New Therapy and Investigating Potential Mechanisms of Resistance.” 2012. Web. 01 Mar 2021.
Vancouver:
Sullivan LA. Anti-Angiogenic Therapy in Non-Small Cell Lung Cancer: Characterizing a New Therapy and Investigating Potential Mechanisms of Resistance. [Internet] [Thesis]. University of Texas Southwestern Medical Center; 2012. [cited 2021 Mar 01].
Available from: http://hdl.handle.net/2152.5/1040.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Sullivan LA. Anti-Angiogenic Therapy in Non-Small Cell Lung Cancer: Characterizing a New Therapy and Investigating Potential Mechanisms of Resistance. [Thesis]. University of Texas Southwestern Medical Center; 2012. Available from: http://hdl.handle.net/2152.5/1040
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
University of Texas Southwestern Medical Center
29.
Dean, Victoria Nicole.
Effect of Physical Activity on Community Participation among Breast Cancer Patients.
Degree: 2015, University of Texas Southwestern Medical Center
URL: http://hdl.handle.net/2152.5/1717
► BACKGROUND: Breast cancer patients who engage in physical activity are shown to have a reduction in difficulties during daily living as a result of treatment…
(more)
▼ BACKGROUND: Breast cancer patients who engage in physical activity are shown to have a reduction in difficulties during daily living as a result of treatment side effects; however, it is unknown if participation in physical activity will reduce the limitations experienced by these individuals while participating in community activities.
SUBJECTS: Female breast cancer patients, with a mean age of 51.81(SD = 7.88, range 30-64), diagnosed with stage 1 (n = 16, 21.3%), stage II (n = 37, 49.3%), stage III (n = 15, 20.0%) cancer who have completed chemotherapy (n = 63, 84.0%), are undergoing chemotherapy (n = 9, 12.0%), or have not yet started chemotherapy (n = 1, 1.3%). The majority of the participants were individuals were employed full time (n = 39, 52.0%), employed part time (n = 13, 17.3%). The majority of these women were married (n = 49) or divorced (n = 11). These women were college graduates (n = 30) or finished some graduate school (n = 20). These participants were European American (n = 34, 45.3%) and African American (n = 13, 17.3%). The BMI of these participants had a mean of 27.28 with a range from 16.30 to 44.81(SD = 5.35). Some of these individuals (n = 53) reported co-occuring medical conditions such as high cholesterol, high blood pressure, diabetes, and being overweight.
METHOD: We recruited female breast cancer patients between the age of 18-66, with stage I, II, or III cancer, who are starting, in the process of, or have completed chemotherapy. Participants who met the inclusion criteria were selected to participate. Research assistants invited these patients to volunteer the survey study. For the current study purpose, we analyzed the association between the International Physical Activity Questionnaire (IPAQ) and the World Health Organization Disability Assessment Scale version 2.0 (WHODAS 2.0), using the Pearson product-moment correlation at the level of statistical significance at .05. The survey packages were sent to the participants through the United States Postal Service. Once the surveys were returned by mail the participants received a $10 gift card for compensation.
RESULTS: A significant negative association was observed between limitations in participation in society (r = -.31, p =.004, medium effect size) and walking. Significant negative associations were also discovered between the limitations in life activities and vigorous activity (r = -.24, p = .023, small to medium effect size), along with walking (r = -.23, p = 0.25, small to medium effect size). Lastly, a significant negative association was seen between the limitations in communication and understanding and walking (r = -.20, p = .046, small to medium effect size).
DISCUSSION: Engaging in physical activity decreases the limitations in understanding and communication, life activities, and participation in society an individual encounters. Vigorous activity is not required to experience reductions in community limitations. Simply engaging in light physical activity, like walking, produces results.
Advisors/Committee Members: Casenave, Gerald W., Chiu, Chung-Yi, Rose, Lindsey.
Subjects/Keywords: Breast Neoplasms; Questionnaires; Walking
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APA ·
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CSE |
Export
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APA (6th Edition):
Dean, V. N. (2015). Effect of Physical Activity on Community Participation among Breast Cancer Patients. (Thesis). University of Texas Southwestern Medical Center. Retrieved from http://hdl.handle.net/2152.5/1717
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Dean, Victoria Nicole. “Effect of Physical Activity on Community Participation among Breast Cancer Patients.” 2015. Thesis, University of Texas Southwestern Medical Center. Accessed March 01, 2021.
http://hdl.handle.net/2152.5/1717.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Dean, Victoria Nicole. “Effect of Physical Activity on Community Participation among Breast Cancer Patients.” 2015. Web. 01 Mar 2021.
Vancouver:
Dean VN. Effect of Physical Activity on Community Participation among Breast Cancer Patients. [Internet] [Thesis]. University of Texas Southwestern Medical Center; 2015. [cited 2021 Mar 01].
Available from: http://hdl.handle.net/2152.5/1717.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Dean VN. Effect of Physical Activity on Community Participation among Breast Cancer Patients. [Thesis]. University of Texas Southwestern Medical Center; 2015. Available from: http://hdl.handle.net/2152.5/1717
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
University of Texas Southwestern Medical Center
30.
McEllin, Brian Matthew.
Dissecting Molecular Mechanisms of Radioresistance Using in Vitro and in Vivo Brain Tumor Model Systems.
Degree: 2012, University of Texas Southwestern Medical Center
URL: http://hdl.handle.net/2152.5/1000
► Glioblastoma multiforme (GBM) are deadly brain tumors that are refractory to radiation and chemotherapy. Despite decades of work, little progress has been made in improving…
(more)
▼ Glioblastoma multiforme (GBM) are deadly brain tumors that are refractory to radiation and chemotherapy. Despite decades of work, little progress has been made in improving patient outcomes. Recent mapping of the GBM genome by the Cancer Genome Atlas Network revealed that these cancers commonly exhibit several signature mutations that promote gliomagenesis (e.g. EGFR amplification/activation, PTEN loss, p53 loss, Ink4a/Arf loss). How these genetic changes may modulate responses to radiation and chemotherapy is not well understood. To elucidate this relationship, genetically defined mouse models have been used for both in vitro and in vivo analysis.
Work has uncovered novel links between oncogenic signaling and DNA repair pathways. First, activation of the Akt pathway by EGFRvIII, a constitutively active form of EGFR, promotes DNA double strand break repair by non-homologous end joining in astrocytes and glioma cell lines. This results in faster repair and increased radioresistance, both in vitro and in orthotopic GBM models. While activation of Akt by the loss of PTEN has similar results, data shows that PTEN loss reduces resistance to agents that induce replication-associated DSBs. This phenotype is due to reduced levels of homologous recombination, as astrocytes show increased radial chromosome aberrations and decreased sister chromatid exchanges after PTEN loss. These results have exciting implications, as it has identified two potential new therapeutic strategies for improving treatment in subsets of GBM patients.
The cancer stem cell hypothesis postulates that cancers are organized similar to endogenous stem cell compartments, composed of a self-renewing cancer stem cell and other more “differentiated”, non-stem progeny. To determine how key GBM mutations affect the different cell types in GBM, I used the adult neural stem cell compartment as a reductionist model of a tumor. Surprisingly, data demonstrated that quiescent stem cells showed inherent resistance, even in a wild type mouse. In addition, stem cell-specific p53 loss increases radioresistance only in a subset of non-dividing progenitors, while proliferating progenitors remain sensitive to radiation. This model has offered novel insight into the effect of key pathways deregulated in GBM and how they impact different cell types.
Advisors/Committee Members: Burma, Sandeep, Bachoo, Robert.
Subjects/Keywords: PTEN Phosphohydrolase; Brain Neoplasms; Glioblastoma
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APA ·
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APA (6th Edition):
McEllin, B. M. (2012). Dissecting Molecular Mechanisms of Radioresistance Using in Vitro and in Vivo Brain Tumor Model Systems. (Thesis). University of Texas Southwestern Medical Center. Retrieved from http://hdl.handle.net/2152.5/1000
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
McEllin, Brian Matthew. “Dissecting Molecular Mechanisms of Radioresistance Using in Vitro and in Vivo Brain Tumor Model Systems.” 2012. Thesis, University of Texas Southwestern Medical Center. Accessed March 01, 2021.
http://hdl.handle.net/2152.5/1000.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
McEllin, Brian Matthew. “Dissecting Molecular Mechanisms of Radioresistance Using in Vitro and in Vivo Brain Tumor Model Systems.” 2012. Web. 01 Mar 2021.
Vancouver:
McEllin BM. Dissecting Molecular Mechanisms of Radioresistance Using in Vitro and in Vivo Brain Tumor Model Systems. [Internet] [Thesis]. University of Texas Southwestern Medical Center; 2012. [cited 2021 Mar 01].
Available from: http://hdl.handle.net/2152.5/1000.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
McEllin BM. Dissecting Molecular Mechanisms of Radioresistance Using in Vitro and in Vivo Brain Tumor Model Systems. [Thesis]. University of Texas Southwestern Medical Center; 2012. Available from: http://hdl.handle.net/2152.5/1000
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
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