Advanced search options

Advanced Search Options 🞨

Browse by author name (“Author name starts with…”).

Find ETDs with:

in
/  
in
/  
in
/  
in

Written in Published in Earliest date Latest date

Sorted by

Results per page:

Sorted by: relevance · author · university · dateNew search

You searched for subject:(Myo inositol hexakisphosphate). Showing records 1 – 3 of 3 total matches.

Search Limiters

Last 2 Years | English Only

No search limiters apply to these results.

▼ Search Limiters


University of Adelaide

1. Doolette, Ashlea Louise. Improved techniques for the characterisation of soil organic phosphorus using ³¹P nuclear magnetic resonance spectroscopy and their application to Australian soils.

Degree: 2010, University of Adelaide

Organic phosphorus is potentially an important source of phosphorus (P) for agriculture, although it is not directly available for plant or microbial uptake. However, organic P can be converted into available inorganic P though hydrolysis or mineralisation. The rate of P release from organic P forms depends partly on the specific organic P compounds present in the soil. Until recently characterising soil organic P has been limited by the lack of appropriate analytic techniques. Consequently, organic P dynamics remains poorly understood. In this thesis, the focus was on improving techniques for the characterisation of soil organic P using solution ³¹P nuclear magnetic resonance (NMR) spectroscopy, applying these techniques to characterise a range of Australian soils and developing a better understanding of the cycling and potential bioavailability of soil organic P. The characterisation of soil organic P relies on the correct identification of resonances. Orthophosphate monoester peaks were identified by spiking model organic P compounds into NaOH- EDTA soil extracts. In this way, seven major resonances that were common to most of the NMR spectra were assigned to adenosine-monophosphate (AMP), scyllo-inositol hexakisphosphate, α- and β-glycerophosphate and myo-inositol hexakisphosphate (phytate). More importantly, spiking highlighted the similarly in appearance and chemical shift of some of the orthophosphate monoester resonances, particularly those of phytate and α- and β-glycerophosphate. This may have resulted in the misidentification and over-estimation of the concentrations of these species in previous studies. To provide a detailed quantitative assessment of soil organic P using ³¹P NMR spectroscopy, a modified method of spectral deconvolution, which included using an internal standard (methylenediphosphonic acid; MDP), was developed. The method of deconvolution implemented in this thesis considered P contained in larger humic molecules. A broad signal, in addition to the routinely fitted sharp peaks, was fitted to the orthophosphate monoester region of the NMR spectrum. A large proportion of monoester P (32–78%) could be assigned to this signal. When the broad signal was not taken into account phytate concentrations were over-estimated by 54%. It is likely that the concentrations of other specific orthophosphate monoester compounds were also over-estimated. The potential over-estimation of phytate concentrations has implication for the understanding of phytate stability in soils. High phytate concentrations in soils are usually explained by the stability of phytate in soils or the limited presence or activity of specialised enzymes (phytase). Lower phytate concentrations suggest phytate maybe less stable in soils than previously supposed. Therefore, the rate of phytate degradation in a calcareous soil was investigated. Phytate was applied to a calcareous soil at four different concentrations (ranging from 58–730 mg kg⁻¹) and the effect of wheat straw as an additional source of carbon was also examined.… Advisors/Committee Members: Smernik, Ronald Josef (advisor), Dougherty, Warwick John (advisor), School of Agriculture, Food and Wine (school).

Subjects/Keywords: ³¹P nuclear magnetic resonance (NMR) spectroscopy; organic phosphorus; myo-inositol hexakisphosphate (phytate)

Record DetailsSimilar RecordsGoogle PlusoneFacebookTwitterCiteULikeMendeleyreddit

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6th Edition):

Doolette, A. L. (2010). Improved techniques for the characterisation of soil organic phosphorus using ³¹P nuclear magnetic resonance spectroscopy and their application to Australian soils. (Thesis). University of Adelaide. Retrieved from http://hdl.handle.net/2440/65555

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16th Edition):

Doolette, Ashlea Louise. “Improved techniques for the characterisation of soil organic phosphorus using ³¹P nuclear magnetic resonance spectroscopy and their application to Australian soils.” 2010. Thesis, University of Adelaide. Accessed January 17, 2021. http://hdl.handle.net/2440/65555.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7th Edition):

Doolette, Ashlea Louise. “Improved techniques for the characterisation of soil organic phosphorus using ³¹P nuclear magnetic resonance spectroscopy and their application to Australian soils.” 2010. Web. 17 Jan 2021.

Vancouver:

Doolette AL. Improved techniques for the characterisation of soil organic phosphorus using ³¹P nuclear magnetic resonance spectroscopy and their application to Australian soils. [Internet] [Thesis]. University of Adelaide; 2010. [cited 2021 Jan 17]. Available from: http://hdl.handle.net/2440/65555.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Doolette AL. Improved techniques for the characterisation of soil organic phosphorus using ³¹P nuclear magnetic resonance spectroscopy and their application to Australian soils. [Thesis]. University of Adelaide; 2010. Available from: http://hdl.handle.net/2440/65555

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

2. Luciana de Paula Naves. Activity of microbial phytases in different conditions of storage, pH, temperature and thermic processes.

Degree: 2009, UNIVERSIDADE FEDERAL DE LAVRAS

O uso de fitase na nutriÃÃo das aves à cada vez mais frequente e promissor. Entretanto, hà vÃrias fitases disponÃveis no mercado e poucos estudos cientÃficos sobre a influÃncia da forma e tempo de armazenamento, pH, temperatura e processamento tÃrmico na atividade da enzima em questÃo. Portanto, este experimento foi realizado com trÃs fitases de diferentes origens (Aspergillus oryzae, Aspergillus niger e Saccharomyces cerevisae) e dividido em trÃs etapas com os objetivos de: (1) avaliar o efeito de duas formas de armazenamento sobre a atividade das fitases; (2) avaliar os efeitos do pH e temperatura, in vitro, sobre a atividade das fitases; e (3) avaliar o efeito de trÃs tempos de exposiÃÃo ao calor sobre a atividade das fitases incorporadas a dois tipos de raÃÃes para frangos de corte, durante um perÃodo mÃximo de armazenamento de 60 dias em temperatura ambiente (TA). Na primeira etapa, as fitases foram armazenadas nas seguintes formas, em quatro repetiÃÃes cada: a) original (como adquirida) com atividade catalÃtica determinada apÃs 0; 60; 90; 120; 150 e 180 dias de armazenamento em TA; e b) incorporada a um nÃcleo contendo vitaminas, macro e microminerais e aminoÃcidos, com determinaÃÃo da atividade a cada 30 dias de armazenamento em TA, durante 180 dias. Na segunda, a atividade foi determinada, em quatro repetiÃÃes, em valores de pH de 2,0 a 9,0 e em temperaturas de 20 a 90ÂC. A Ãltima etapa foi dividida em dois experimentos, com trÃs repetiÃÃes cada. Experimento 1: avaliou-se o efeito de dois tipos de raÃÃo para frangos de corte (fase inicial e final) e quatro tratamentos (raÃÃo controle e submetidas a trÃs tempos de exposiÃÃo ao calor) sobre a atividade das fitases. Experimento 2: a raÃÃo de fase inicial submetida aos quatro tratamentos citados anteriormente teve a atividade de fitase determinada apÃs 0; 15; 30; 45 e 60 dias de armazenamento em TA. As determinaÃÃes da atividade da fitase de S. cerevisae nÃo apresentaram padrÃes de repetibilidade durante a primeira etapa, portanto, essa enzima foi avaliada apenas na segunda etapa. Houve perda linear de atividade enzimÃtica, durante o armazenamento, em ambas as formas avaliadas. A fitase de A. niger foi a enzima com menor perda de atividade nas duas formas de armazenamento. O armazenamento, em TA, das fitases na forma original à o mais indicado. VariaÃÃes no pH e temperatura acarretaram em alteraÃÃes na atividade das trÃs fitases. A fitase de A. oryzae teve maior atividade no pH 4,0 enquanto as outras fitases apresentaram pH Ãtimo entre 4,5 e 5,0. A fitase de A. oryzae apresentou maior atividade a 40ÂC e a 50 °C estava inativa, enquanto que a fitase de A. niger teve maior atividade prÃximo a 45ÂC, estando inativa a 60 °C. A fitase de S. cerevisae foi a mais termoresistente, pois estava ativa a 60ÂC, com atividade mÃxima entre 50 e 60ÂC. Os resultados obtidos nos experimentos 1 e 2 da terceira etapa mostraram que a atividade enzimÃtica nÃo foi alterada pelo tipo de raÃÃo, processamento tÃrmico das raÃÃes nem pelo armazenamento das mesmas, em TA, durante 60… Advisors/Committee Members: Angelita Duarte CorrÃa, Matheus Puggina de Freitas, Paulo Borges Rodrigues.

Subjects/Keywords: myo-inositol hexakisphosphate phosphohydrolase; Ãcido fÃtico; phytate; frango de corte; suplementaÃÃo; fitato; suplementation; hexafosfato de mio-inositol fosfo-hidrolase.; phytic acid; broiler chicken; ZOOTECNIA; atividade enzimÃtica; enzyme activity

Record DetailsSimilar RecordsGoogle PlusoneFacebookTwitterCiteULikeMendeleyreddit

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6th Edition):

Naves, L. d. P. (2009). Activity of microbial phytases in different conditions of storage, pH, temperature and thermic processes. (Thesis). UNIVERSIDADE FEDERAL DE LAVRAS. Retrieved from http://bibtede.ufla.br/tede//tde_busca/arquivo.php?codArquivo=2005

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16th Edition):

Naves, Luciana de Paula. “Activity of microbial phytases in different conditions of storage, pH, temperature and thermic processes.” 2009. Thesis, UNIVERSIDADE FEDERAL DE LAVRAS. Accessed January 17, 2021. http://bibtede.ufla.br/tede//tde_busca/arquivo.php?codArquivo=2005.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7th Edition):

Naves, Luciana de Paula. “Activity of microbial phytases in different conditions of storage, pH, temperature and thermic processes.” 2009. Web. 17 Jan 2021.

Vancouver:

Naves LdP. Activity of microbial phytases in different conditions of storage, pH, temperature and thermic processes. [Internet] [Thesis]. UNIVERSIDADE FEDERAL DE LAVRAS; 2009. [cited 2021 Jan 17]. Available from: http://bibtede.ufla.br/tede//tde_busca/arquivo.php?codArquivo=2005.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Naves LdP. Activity of microbial phytases in different conditions of storage, pH, temperature and thermic processes. [Thesis]. UNIVERSIDADE FEDERAL DE LAVRAS; 2009. Available from: http://bibtede.ufla.br/tede//tde_busca/arquivo.php?codArquivo=2005

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

3. Wu, Di. Discovery and characterization of a signaling molecule regulating somatic embryogenesis in loblolly pine.

Degree: PhD, Chemistry and Biochemistry, 2008, Georgia Tech

myo-Inositol-1,2,3,4,5,6-hexakisphosphate (InsP6), also called phytic acid, is ubiquitous in eukaryotic cells and the most abundant inositol phosphate derivative. Loblolly pine (LP, Pinus taeda) constitutes the primary commercial species in the southern forest of U.S. Somatic embryogenesis (SE) is an effective technique to maintain the desirable genetic composition of the progeny and to accomplish the efficiency of propagation. SE can also serve as a tool for study of plant development. Unlike angiosperm embryos with attached cotyledons as seed storage organs, the diploid conifer embryo is surrounded by the unattached haploid female gametophyte (FG). In LP SE, FG tissue is absent in the embryogenic tissue culture. We found that extracts from early-stage FG stimulate growth and multiplication of early-stage somatic embryos, whereas FG water extracts from late stage contain substance(s) inhibitory to early-stage somatic embryo growth (DeSilva et al., 2007). We now present the isolation and identification of the inhibitory substance as InsP6 by means of water extraction, two gel filtrations and two ion exchange FPLC chromatographies. The results represent the first complete structural characterization of InsP6 from a natural product using LC/MS, LC/MS/MS, exact MS, 1D- and 2D-NMR analyses. We also report that there is a good correlation between the amount of InsP6 purified from FG tissue (1.3 nmoles per full-term FG) and the amount of InsP6 which inhibits somatic embryo growth. This novel approach of isolating and characterizing InsP6 from plant tissue, and investigating its role on SE can allow us to improve SE technology by circumventing current bottleneck, to elucidate enigmatic functions of InsP6 in plants, and most importantly, to utilize this molecule properly. Advisors/Committee Members: Dr. Sheldon May (Committee Chair), Dr. Donald Doyle (Committee Member), Dr. Gerald Pullman (Committee Member), Dr. James Powers (Committee Member), Dr. Nicholas Hud (Committee Member).

Subjects/Keywords: NMR; LC/MS; FPLC; Female gametophyte; Loblolly pine; Somatic embryogenesis; Myo-inositol hexakisphosphate; Loblolly pine; Somatic embryogenesis; Phytic acid

…vivo of myo-inositol hexakisphosphate…. .150 Impact of the discovery and future work… …SYMBOLS AND ABBREVIATIONS InsP6 myo-Inositol-1,2,3,4,5,6-hexakisphosphate SE Somatic… …27 1 MLWGVLFQFMWAVVSLVVTALVACWAK27 myo-Inositol-1,2,3,4,5,6-hexakisphosphate History… …background myo-Inositol-1,2,3,4,5,6-hexakisphosphate (InsP6), also called phytic acid, is… …and 1990s (Sasakawa et al., 1995). myo-Inositol-1,2,3,4,5,6-hexakisphosphate in… 

Record DetailsSimilar RecordsGoogle PlusoneFacebookTwitterCiteULikeMendeleyreddit

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6th Edition):

Wu, D. (2008). Discovery and characterization of a signaling molecule regulating somatic embryogenesis in loblolly pine. (Doctoral Dissertation). Georgia Tech. Retrieved from http://hdl.handle.net/1853/28252

Chicago Manual of Style (16th Edition):

Wu, Di. “Discovery and characterization of a signaling molecule regulating somatic embryogenesis in loblolly pine.” 2008. Doctoral Dissertation, Georgia Tech. Accessed January 17, 2021. http://hdl.handle.net/1853/28252.

MLA Handbook (7th Edition):

Wu, Di. “Discovery and characterization of a signaling molecule regulating somatic embryogenesis in loblolly pine.” 2008. Web. 17 Jan 2021.

Vancouver:

Wu D. Discovery and characterization of a signaling molecule regulating somatic embryogenesis in loblolly pine. [Internet] [Doctoral dissertation]. Georgia Tech; 2008. [cited 2021 Jan 17]. Available from: http://hdl.handle.net/1853/28252.

Council of Science Editors:

Wu D. Discovery and characterization of a signaling molecule regulating somatic embryogenesis in loblolly pine. [Doctoral Dissertation]. Georgia Tech; 2008. Available from: http://hdl.handle.net/1853/28252

.