subject:(Mutans)

Universidade Estadual de Campinas

1. Vizoto, Natália Leal, 1982-. Avaliação da função biológica do sistema de dois componentes SptRS de Streptococcus mutans: Evaluation of biological function of Streptococcus mutans two component system SptRS.

Degree: 2015, Universidade Estadual de Campinas

URL: http://repositorio.unicamp.br/jspui/handle/REPOSIP/288675

► Abstract: Streptococcus mutans is a common bacterial species of the bucal microbiota of humans involved in the pathogenesis of dental caries and infectious endocarditis promoted… (more)

▼ Abstract: Streptococcus mutans is a common bacterial species of the bucal microbiota of humans involved in the pathogenesis of dental caries and infectious endocarditis promoted by bacteremia of bucal origin. To be transmitted and occupy their ecological niches, S. mutans need to persist in saliva and adapt physiologically to each phase of colonization, a process that probably involves several changes in its transcriptome. To this end, S. mutans uses transcriptional regulatory systems called Two Component System (TCS). The TCS SptRS was identified in an in silico analysis of the genome of S. mutans strain UA159, as an orthologue of the SptRS system (Spt of Saliva persistence) of Streptococcus pyogenes. The aim of this study was to investigate the role of the TCS SptRS in functional traits important for S. mutans to colonize its bucal niches. Thus, knockout mutants of sptR and sptS (SMU.927 and SMU.928, respectively) were obtained in strain UA159 (UAsptR-, UAsptS-) and compared to parental strain regarding morphology, planktonic growth under different nutritional conditions and persistence in human saliva, biofilm formation and autolysis at 44 °C. In addition, genes of SptRS regulon were analised by Chromatin Immunoprecipitation followed the sequencing (ChIP-seq), quantitative RT-PCR (RT- qPCR) and Electrophoretic Mobility Shift Assays (EMSA) with S. mutans SptR recombinant protein. Inactivation of sptR/S promotes slow planktonic growth in RPMI and CDM, culture media 22.4 to 53.13% reductions in autolysis respectively, but does not significantly affect morphogenesis. However, mutants do not show significant alterations in biofilm formation or in persistence in human saliva. ChIP-seq and RT-qPCR analyses showed that SptRS regulates genes for stringent response (SMU.926), metabolism of multiple sugars (SMU.78, SMU.137, SMU.542, SMU.1734), catabolite repression (SMU.1591, ccpA), phosphoenolpyruvate phosphotransferase systems (PTS), ABC transport systems (SMU.182, SMU.880, SMU.905, SMU.1035, SMU.1095, SMU.1178c, SMU.1939) and cell wall biogenesis (SMU.22, SMU.609, SMU.1091, SMU.1434c, SMU.2147) SptR worked as a negative regulator of 86% (37/43) of the tested genes. RT-qPCR and EMSA analyses further showed that SptR directly represses expression of the transcriptional regulator CovR (SMU.1924), which is a repressor of genes involved in biofilm formation and virulence. This study provides evidence that SptRS regulates several functions important for S. mutans survival under poor nutritional conditions, apparently coordinating metabolism with bacterial growth and expression of virulence genes Advisors/Committee Members: UNIVERSIDADE ESTADUAL DE CAMPINAS (CRUESP), Mattos-Graner, Renata de Oliveira, 1971- (advisor), Universidade Estadual de Campinas. Faculdade de Odontologia de Piracicaba (institution), Programa de Pós-Graduação em Biologia e Patologia Buco-Dental (nameofprogram), Klein, Marlise Inez (committee member), Camargo, Tarsila Mendes de (committee member), Duque, Cristiane (committee member), Boriollo, Marcelo Fabiano Gomes (committee member).

Subjects/Keywords: Streptococcus mutans; Streptococcus mutans

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APA (6^th Edition):

Vizoto, Natália Leal, 1. (2015). Avaliação da função biológica do sistema de dois componentes SptRS de Streptococcus mutans: Evaluation of biological function of Streptococcus mutans two component system SptRS. (Thesis). Universidade Estadual de Campinas. Retrieved from http://repositorio.unicamp.br/jspui/handle/REPOSIP/288675

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Vizoto, Natália Leal, 1982-. “Avaliação da função biológica do sistema de dois componentes SptRS de Streptococcus mutans: Evaluation of biological function of Streptococcus mutans two component system SptRS.” 2015. Thesis, Universidade Estadual de Campinas. Accessed January 24, 2021. http://repositorio.unicamp.br/jspui/handle/REPOSIP/288675.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Vizoto, Natália Leal, 1982-. “Avaliação da função biológica do sistema de dois componentes SptRS de Streptococcus mutans: Evaluation of biological function of Streptococcus mutans two component system SptRS.” 2015. Web. 24 Jan 2021.

Vancouver:

Vizoto, Natália Leal 1. Avaliação da função biológica do sistema de dois componentes SptRS de Streptococcus mutans: Evaluation of biological function of Streptococcus mutans two component system SptRS. [Internet] [Thesis]. Universidade Estadual de Campinas; 2015. [cited 2021 Jan 24]. Available from: http://repositorio.unicamp.br/jspui/handle/REPOSIP/288675.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Vizoto, Natália Leal 1. Avaliação da função biológica do sistema de dois componentes SptRS de Streptococcus mutans: Evaluation of biological function of Streptococcus mutans two component system SptRS. [Thesis]. Universidade Estadual de Campinas; 2015. Available from: http://repositorio.unicamp.br/jspui/handle/REPOSIP/288675

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Universidad de Chile

2. Tournelle Kimura, Pamela Madeleine. Prevalencia y diversidad de bacterias pertenecientes al género Streptococcus en saliva de niños pre-escolares chilenos entre 2 y 5 años de edad con y sin caries.

Degree: 2013, Universidad de Chile

URL: http://repositorio.uchile.cl/handle/2250/117563

► Introducción: La cavidad oral es un ecosistema mixto, formado por diversas especies microbianas, en donde las bacterias cumplen un rol fundamental en el equilibrio salud-… (more)

▼ Introducción: La cavidad oral es un ecosistema mixto, formado por diversas especies microbianas, en donde las bacterias cumplen un rol fundamental en el equilibrio salud- enfermedad. Se ha descrito a la caries dental como una de las patologías orales más prevalentes a nivel mundial y se ha observado que especies pertenecientes al género Streptococcus, específicamente al grupo viridans, están fuertemente relacionadas con su desarrollo. S. mutans ha sido una de las especies más asociadas a su inicio y progresión. Sin embargo, esta enfermedad puede ocurrir en ausencia de él y, otras especies pertenecientes a este género, podrían tener una inesperada relevancia en la enfermedad. Objetivo: Determinar la prevalencia y diversidad de especies bacterianas pertenecientes al género Streptococcus en saliva de niños pre-escolares chilenos con y sin caries. Metodología: Muestras de saliva total no estimulada fueron colectadas de la cavidad oral de niños pre-escolares chilenos, entre 2 y 5 años de edad, que presentan caries (10 individuos) o libres de caries (10 individuos), según índice ICDAS II. Se determinó la capacidad tamponante y pH salival, recuento, aislamiento e identificación bacteriana pertenecientes al género Streptococcus. La identificación se realizó mediante test bioquímicos, Reacción en Cadena de la Polimerasa (PCR) y secuenciación de ADN, según corresponda. Resultados: Se observaron diferencias en relación a las especies identificadas en las muestras de sujetos con y sin caries. De los aislados obtenidos de sujetos sin caries, las especies más prevalentes fueron S. salivarius (40%), S. mutans (20%) y S. thermophilus (13,3%) y las especies aisladas en baja proporción correspondieron a S. vestibularis y S. sanguinis (3,3% cada una). De los aislados obtenidos de sujetos con caries, la especie más prevalente correspondió a S. salivarius (73,3%) y las especies encontradas en menor proporción correspondieron a Bacillus subtilis subsp. subtilis (16,7%), S. anginosus y S. mitis (3,3% cada una). Los análisis estadísticos para asociar las variables edad, pH, capacidad tamponante y recuento bacteriano, en relación a prevalencia de caries, resultaron ser no significativos. 2 Conclusiones: Las especies del género Streptococcus y otras especies identificadas, tanto para sujetos con y sin caries, fueron diferentes en cuanto a diversidad. Sin embargo, especies que han sido identificadas como autores principales tanto del desarrollo de caries como de su ausencia, fueron encontradas en baja proporción o no fueron encontradas en este grupo etario. S. salivarius resultó ser la especie aislada con mayor frecuencia en ambos grupos y, parámetros como edad, pH, capacidad tamponante en relación con prevalencia de caries, no fueron significativos. Esto podría revelar que las propiedades cariogénicas y de virulencia de cada especie estarían en íntima relación con las condiciones ambientales y las asociaciones bacterianas que ellas realicen dentro del Biofilm, que una misma especie podría…

Subjects/Keywords: Streptococcus mutans

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Tournelle Kimura, P. M. (2013). Prevalencia y diversidad de bacterias pertenecientes al género Streptococcus en saliva de niños pre-escolares chilenos entre 2 y 5 años de edad con y sin caries. (Thesis). Universidad de Chile. Retrieved from http://repositorio.uchile.cl/handle/2250/117563

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Tournelle Kimura, Pamela Madeleine. “Prevalencia y diversidad de bacterias pertenecientes al género Streptococcus en saliva de niños pre-escolares chilenos entre 2 y 5 años de edad con y sin caries.” 2013. Thesis, Universidad de Chile. Accessed January 24, 2021. http://repositorio.uchile.cl/handle/2250/117563.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Tournelle Kimura, Pamela Madeleine. “Prevalencia y diversidad de bacterias pertenecientes al género Streptococcus en saliva de niños pre-escolares chilenos entre 2 y 5 años de edad con y sin caries.” 2013. Web. 24 Jan 2021.

Vancouver:

Tournelle Kimura PM. Prevalencia y diversidad de bacterias pertenecientes al género Streptococcus en saliva de niños pre-escolares chilenos entre 2 y 5 años de edad con y sin caries. [Internet] [Thesis]. Universidad de Chile; 2013. [cited 2021 Jan 24]. Available from: http://repositorio.uchile.cl/handle/2250/117563.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Tournelle Kimura PM. Prevalencia y diversidad de bacterias pertenecientes al género Streptococcus en saliva de niños pre-escolares chilenos entre 2 y 5 años de edad con y sin caries. [Thesis]. Universidad de Chile; 2013. Available from: http://repositorio.uchile.cl/handle/2250/117563

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Université Laval

3. Nicolas, Guillaume. Étude des mutacines.

Degree: 2011, Université Laval

URL: http://hdl.handle.net/20.500.11794/23035

► Face au développement incessant de la résistance aux antibiotiques, la découverte de nouvelles substances antibactériennes devient urgente. Les bactériocines, peptides bactériens à activité antibiotique synthétisés… (more)

▼ Face au développement incessant de la résistance aux antibiotiques, la découverte de nouvelles substances antibactériennes devient urgente. Les bactériocines, peptides bactériens à activité antibiotique synthétisés par les ribosomes, représentent une alternative. Les mutacines sont des bactériocines produites par Streptococcus mutans, une espèce bactérienne indigène de la cavité buccale. Quatre types de mutacines ont été caractérisés à ce jour: les lantibiotiques et non-lantibiotiques, monopeptidiques ou dipeptidiques. Des analyses bio-informatiques des génomes de S. mutans UA159 et NN2025 ont révélé la diversité des gènes codant potentiellement pour des bactériocines. L'étude des mutacines s'est toujours confrontée à la difficulté de les produire en milieu liquide. Deux mutacines (F-59.1 et I-T9) ont été produites et isolées d'un surnageant de culture d'un milieu élaboré à partir de perméat de lactosérum. La mutacine D-123.1 a été isolée à partir d'un milieu de culture de nature semi-solide. Les analyses biochimiques et moléculaires des mutacines révèlent que la mutacine F-59.1 est apparentée aux bactériocines de la famille des pédiocines alors que la mutacine D-123.1 appartient aux lantibiotiques. Les concentrations minimales inhibitrices (CMI) et bactéricides (CMB) des mutacines D-123.1 et F-59.1 ont été déterminées contre plusieurs pathogènes humains. La mutacine D-123.1 a montré son efficacité contre les pathogènes du domaine médical et alimentaire avec des CMI comprises entre 0.25 et 4 ug/mL. La mutacine F-59.1 inhibe les pathogènes alimentaires avec des CMI comprises entre 3.2 et 12.8 ug/mL. De façon à détecter simplement et rapidement des mutacines apparentées aux pédiocines, les 24 souches type productrices de mutacines ont été testées par un test d'antagonisme différé, pour leur capacité à produire une mutacine capable de cibler le complexe IIC des transporteurs membranaires du mannose pour exercer leur activité inihibitrice. Streptococcus salivarius et des mutants défectueux dans leur transporteur du mannose ont été utilisés comme souches indicatrices. Deux souches de S. mutans (T9 et 3B) ont démontré une activité différente contre la souche mère et ces mutants, indiquant potentiellement la production de mutacine ciblant le transporteur du mannose par ces deux souches. Advisors/Committee Members: Lapointe, Gisèle, Lavoie, Marc C..

Subjects/Keywords: Mutacines; Streptococcus mutans

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Nicolas, G. (2011). Étude des mutacines. (Thesis). Université Laval. Retrieved from http://hdl.handle.net/20.500.11794/23035

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Nicolas, Guillaume. “Étude des mutacines.” 2011. Thesis, Université Laval. Accessed January 24, 2021. http://hdl.handle.net/20.500.11794/23035.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Nicolas, Guillaume. “Étude des mutacines.” 2011. Web. 24 Jan 2021.

Vancouver:

Nicolas G. Étude des mutacines. [Internet] [Thesis]. Université Laval; 2011. [cited 2021 Jan 24]. Available from: http://hdl.handle.net/20.500.11794/23035.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Nicolas G. Étude des mutacines. [Thesis]. Université Laval; 2011. Available from: http://hdl.handle.net/20.500.11794/23035

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Rochester

4. Derr, Adam M. NADH Oxidase and Stress Responses in Streptococcus mutans: A Phenotypic and Regulatory Characterization.

Degree: PhD, 2012, University of Rochester

URL: http://hdl.handle.net/1802/25539

► Streptococcus mutans, an oral bacterium found on human tooth surfaces, is a primary causative agent of the disease dental caries. Dental caries is the result… (more)

▼ Streptococcus mutans, an oral bacterium found on human tooth surfaces, is a primary causative agent of the disease dental caries. Dental caries is the result of the demineralization of the tooth surface, partially due to the production of acid by oral bacteria through carbohydrate metabolism. Rapid sugar metabolism in S. mutans depends on the availability of reduced nicotinamide dinucleotides (NADH). A key enzyme for the regeneration of NADH is the flavin-containing NADH oxidase (Nox). This enzyme oxidizes NADH to NAD+, while reducing diatomic oxygen to H2O. In this study, we characterized the role of the NADH oxidase in the oxidative and acid stress responses of S. mutans, determined specific regulatory controls of NADH oxidase and described the global regulatory effects from the loss of nox. The nox mutant strain exhibited reduced ability to metabolize environmental oxygen present in chemostat-grown S. mutans cultures resulting in activation of the oxygen and acid-mediated stress responses, as demonstrated by elevated activity of superoxide dismutase and glutathione oxidoreductase, elevated transcription of DNA repair genes and altered membrane fatty acid composition, independent of external pH. An Spx recognition site was identified within the first 120 bp upstream of the translational start site of the nox-coding region. Measurements of transcription rates from the nox promoter showed that SpxA activates nox and that SpxB slightly inhibits nox, indicating that nox is part of the Spx global regulon. The global effects of the nox mutation and the impacts of oxygen were characterized using cDNA microarrays. Transcriptional patterns were similar between the nox mutant and parent strain, UA159, when exposed to elevated oxygen concentrations, with some exceptions. Notably, in the nox mutant strain, the global regulator Rex, responsible for regulation of oxygen homeostasis, sugar metabolism, and biofilm formation, was differentially regulated, suggesting that Nox and Rex participate in a redox-sensing and signaling pathway. In conclusion, nox is a major consumer of environmental oxygen in S. mutans and the loss of NADH oxidase, or an increase in environmental oxygen, leads to global transcriptional changes, allowing the organism to respond rapidly to stress at the expense of overall growth rate.

Subjects/Keywords: Streptococcus Mutans; NADH Oxidase; Oxygen

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Derr, A. M. (2012). NADH Oxidase and Stress Responses in Streptococcus mutans: A Phenotypic and Regulatory Characterization. (Doctoral Dissertation). University of Rochester. Retrieved from http://hdl.handle.net/1802/25539

Chicago Manual of Style (16^th Edition):

Derr, Adam M. “NADH Oxidase and Stress Responses in Streptococcus mutans: A Phenotypic and Regulatory Characterization.” 2012. Doctoral Dissertation, University of Rochester. Accessed January 24, 2021. http://hdl.handle.net/1802/25539.

MLA Handbook (7^th Edition):

Derr, Adam M. “NADH Oxidase and Stress Responses in Streptococcus mutans: A Phenotypic and Regulatory Characterization.” 2012. Web. 24 Jan 2021.

Vancouver:

Derr AM. NADH Oxidase and Stress Responses in Streptococcus mutans: A Phenotypic and Regulatory Characterization. [Internet] [Doctoral dissertation]. University of Rochester; 2012. [cited 2021 Jan 24]. Available from: http://hdl.handle.net/1802/25539.

Council of Science Editors:

Derr AM. NADH Oxidase and Stress Responses in Streptococcus mutans: A Phenotypic and Regulatory Characterization. [Doctoral Dissertation]. University of Rochester; 2012. Available from: http://hdl.handle.net/1802/25539

Universidad de Chile

5. Jara Contreras, Maximiliano Felipe. Efecto remineralizante de un agente a base de fluor y grafeno, sobre bloques de esmalte desmineralizados con un modelo de biopelícula de Streptococcus mutans.

Degree: 2017, Universidad de Chile

URL: http://repositorio.uchile.cl/handle/2250/146554

► Introducción: La caries es una de las enfermedades más prevalentes a nivel mundial. Se caracteriza por la pérdida de minerales a causa de subproductos ácidos… (more)

▼ Introducción: La caries es una de las enfermedades más prevalentes a nivel mundial. Se caracteriza por la pérdida de minerales a causa de subproductos ácidos liberados por bacterias presentes en la biopelícula adherida a las superficies de los dientes. Esta produce lesiones que pueden culminar en la pérdida de la estructura dentaria, junto con todas las repercusiones que esto genera en la calidad de vida y salud de las personas. Los agentes remineralizantes a base de flúor son productos utilizados para revertir la pérdida de minerales en las etapas iniciales de esta enfermedad. La finalidad de esta investigación radicó en evaluar la capacidad remineralizante de BlueRemin®, un nuevo agente remineralizante a base de flúor y grafeno, con el objetivo de iniciar una línea de investigación que permita evaluar sus propiedades y futuras aplicaciones clínicas. Objetivos: Establecer diferencias en la dureza superficial en especímenes de esmalte desmineralizados mediante un modelo de caries microbiológico y luego de ser sometidos a un protocolo de remineralización con BlueRemin®. Metodología: Se utilizó el modelo de caries descrito por Ccahuana y Cury (2010), que consiste en la simulación de un ambiente cariogénico mediante la exposición de bloques de esmalte a sacarosa, previa formación de una biopelícula monoespecie de Streptococcus mutans, lo cual permitió obtener una zona de desmineralización compatible con una lesión de caries no cavitada que luego fue remineralizada con el agente remineralizador experimental BlueRemin®. Los bloques de esmalte fueron evaluados por microdureza de Vickers antes de la exposición a sacarosa, después de la desmineralización y una vez aplicado el agente remineralizador. Resultados: Las microdurezas de los especímenes de esmalte expuestos al modelo de caries experimentaron un aumento estadísticamente significativo (p < 0.05) luego de ser tratados con el agente remineralizador BlueRemin®. Conclusiones: Debido a que la aplicación de BlueRemin® generó un aumento de la dureza superficial estadísticamente significativo (p < 0.05) de todas las muestras de esmalte, se sugiere que posee capacidad remineralizante.

Subjects/Keywords: Streptococcus mutans; Fluoruros tópicos

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Jara Contreras, M. F. (2017). Efecto remineralizante de un agente a base de fluor y grafeno, sobre bloques de esmalte desmineralizados con un modelo de biopelícula de Streptococcus mutans. (Thesis). Universidad de Chile. Retrieved from http://repositorio.uchile.cl/handle/2250/146554

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Jara Contreras, Maximiliano Felipe. “Efecto remineralizante de un agente a base de fluor y grafeno, sobre bloques de esmalte desmineralizados con un modelo de biopelícula de Streptococcus mutans.” 2017. Thesis, Universidad de Chile. Accessed January 24, 2021. http://repositorio.uchile.cl/handle/2250/146554.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Jara Contreras, Maximiliano Felipe. “Efecto remineralizante de un agente a base de fluor y grafeno, sobre bloques de esmalte desmineralizados con un modelo de biopelícula de Streptococcus mutans.” 2017. Web. 24 Jan 2021.

Vancouver:

Jara Contreras MF. Efecto remineralizante de un agente a base de fluor y grafeno, sobre bloques de esmalte desmineralizados con un modelo de biopelícula de Streptococcus mutans. [Internet] [Thesis]. Universidad de Chile; 2017. [cited 2021 Jan 24]. Available from: http://repositorio.uchile.cl/handle/2250/146554.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Jara Contreras MF. Efecto remineralizante de un agente a base de fluor y grafeno, sobre bloques de esmalte desmineralizados con un modelo de biopelícula de Streptococcus mutans. [Thesis]. Universidad de Chile; 2017. Available from: http://repositorio.uchile.cl/handle/2250/146554

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Universidade do Rio Grande do Sul

6. Damé-Teixeira, Nailê. Efeito da redução do aporte nutricional na diversidade genotípica e nos fatores de virulência de bactérias cariogênicas isoladas de dentina cariada selada.

Degree: 2015, Universidade do Rio Grande do Sul

URL: http://hdl.handle.net/10183/130444

►

No tratamento de lesões profundas de cárie, a Remoção Parcial de Dentina Cariada (RPDC) e restauração tem sido proposta como alternativa conservadora para evitar perda… (more)

▼

No tratamento de lesões profundas de cárie, a Remoção Parcial de Dentina Cariada (RPDC) e restauração tem sido proposta como alternativa conservadora para evitar perda de tecido dentário e exposição pulpar. Existe uma hipótese de que uma seleção de bactérias ocorre abaixo de restaurações devido a um acesso limitado de nutrientes. No entanto, há falta de conhecimento sobre a diversidade e potencial de virulência das bactérias cariogênicas residuais seladas abaixo de restaurações sobre dentina cariada. O objetivo deste estudo foi caracterizar Streptococcus mutans e lactobacilos isolados de dentina cariada antes e após o estresse nutricional por selamento da cavidade. S. mutans e lactobacilos foram obtidos por cultivo da dentina cariada de lesões cavitadas de quatro e seis pacientes, respectivamente. Duas amostras de dentina cariada foram coletadas e cultivadas por paciente: uma antes e outra após três meses de selamento da cavidade. Colônias de S. mutans e lactobacilos preditos foram selecionadas, isoladas e analisadas por coloração de Gram. Genes “housekeeping” foram utilizados na identificação da espécie (gtfB para S. mutans e pheS/rpoA/groEL/16SrRNA para lactobacilos) e a técnica de AP-PCR foi utilizada para genotipagem. A análise fenotípica (produção de ácido e de tolerância ao ácido) foi realizada. Um total de 48 isolados representativos de S. mutans foram analisados (31 antes e 17 após estresse nutricional por meio do selamento). O número de genótipos diferentes de S. mutans encontrado foi de nove e seis antes e após selamento, respectivamente. Pelo menos um dos genótipos encontrados antes do selamento foi também encontrado na dentina após o estresse nutricional por meio do selamento. Em relação aos lactobacilos, analisou-se 86 cepas, 41 antes e 45 após estresse nutricional. L. paracasei e L. rhamnosus prevaleceram e apenas quatro isolados não pertenciam a estas espécies. Um total de 27 e 15 genótipos de lactobacilos diferentes foram encontrados antes e após selamento, respectivamente. Não houve diferença entre isolados de S. mutans e lactobacilos de dentina cariada antes e após o estresse nutricional na produção de ácido ou tolerância ao ácido. L. paracasei apresentaram menor valor de pH em 48h de crescimento. Em conclusão, a diversidade genotípica diminuiu após o estresse nutricional, mas a virulência de S. mutans e lactobacilos permaneceu a mesma. L. rhamnosus foi selecionado após o estresse nutricional. Algumas espécies de lactobacilos apareceram após o selamento, sugerindo pressão seletiva no sítio antes do selamento pela alta disponibilidade de nutrientes. Mais estudos avaliando outras características de virulência são necessários para entender melhor a cariogenicidade das cepas residuais seladas abaixo de restaurações e para compreender a resistência de L. rhamnosus a este sítio.

A hypothesis exists that a selection of bacteria occurs underneath restoration due to a limited access of nutrients. However, there is a lack of evidence regarding their role in the progression of carious process beneath…

Advisors/Committee Members: Maltz, Marisa.

Subjects/Keywords: Cárie dentária; Streptococcus mutans; Lactobacilos

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Damé-Teixeira, N. (2015). Efeito da redução do aporte nutricional na diversidade genotípica e nos fatores de virulência de bactérias cariogênicas isoladas de dentina cariada selada. (Thesis). Universidade do Rio Grande do Sul. Retrieved from http://hdl.handle.net/10183/130444

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Damé-Teixeira, Nailê. “Efeito da redução do aporte nutricional na diversidade genotípica e nos fatores de virulência de bactérias cariogênicas isoladas de dentina cariada selada.” 2015. Thesis, Universidade do Rio Grande do Sul. Accessed January 24, 2021. http://hdl.handle.net/10183/130444.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Damé-Teixeira, Nailê. “Efeito da redução do aporte nutricional na diversidade genotípica e nos fatores de virulência de bactérias cariogênicas isoladas de dentina cariada selada.” 2015. Web. 24 Jan 2021.

Vancouver:

Damé-Teixeira N. Efeito da redução do aporte nutricional na diversidade genotípica e nos fatores de virulência de bactérias cariogênicas isoladas de dentina cariada selada. [Internet] [Thesis]. Universidade do Rio Grande do Sul; 2015. [cited 2021 Jan 24]. Available from: http://hdl.handle.net/10183/130444.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Damé-Teixeira N. Efeito da redução do aporte nutricional na diversidade genotípica e nos fatores de virulência de bactérias cariogênicas isoladas de dentina cariada selada. [Thesis]. Universidade do Rio Grande do Sul; 2015. Available from: http://hdl.handle.net/10183/130444

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Universidad de Chile

7. Sieber Carrasco, Carolina Verónica. Recuento de Streptococcus mutans en muestras de biofilm sobre dientes restaurados con resina compuesta oclusal versus dientes sanos mediante el método de cubeta.

Degree: 2012, Universidad de Chile

URL: http://repositorio.uchile.cl/handle/2250/111754

► Introducción: Determinar el riesgo cariogénico del paciente es un requisito fundamental para efectuar un adecuado diagnóstico de salud bucal. Establecer el recuento de Streptococcus mutans… (more)

▼ Introducción: Determinar el riesgo cariogénico del paciente es un requisito fundamental para efectuar un adecuado diagnóstico de salud bucal. Establecer el recuento de Streptococcus mutans (S.mutans) sobre piezas dentarias puede permitir identificar el nivel de riesgo microbiológico en desarrollar caries, y en el caso de piezas restauradas, caries secundarias, principal causa de fallas de restauraciones, evitando el futuro recambio de ellas. Conocer la colonización de microbiota cariogénica en restauraciones dentarias y dientes sanos, podría ser un aspecto a considerar en las decisiones de tratamiento, posibilitando la selección de un material de obturación y medidas preventivas, ajustada con el riesgo cariogénico local y propio de cada paciente. Material y Método: Se seleccionaron 69 pacientes de la clínica de Operatoria 4to año de la Facultad de Odontología de la Universidad de Chile durante el período de septiembre a diciembre del año 2011. En cada uno de ellos se tomó una muestra de placa bacteriana dental de una pieza posterior sana y una restaurada por oclusal con resina compuesta utilizando la técnica de cubeta. Este método consiste en una impresión directa sobre las superficies oclusales de restauraciones, mediante una cubetilla de flúor gel modificada cargada con agar TYCSB. Las cubetas se incubaron en estufa a 37°C por 48 horas, para posteriormente proceder al recuento bacteriano. 2 Resultados: Mediante el método de la cubeta se logró aislar Unidades Formadoras de Colonias (UFC) de S. mutans en dientes con resina compuesta oclusal y en piezas sanas en un 95,6% de las muestras. Se observó una diferencia estadísticamente significativa (p <0,05) donde las muestras de biofilm de placa bacteriana depositada sobre las restauraciones de resina presentaban mayor cantidad de UFC/cm2 que las superficie de piezas sanas. Conclusiones: A partir de muestras de placa bacteriana dental obtenidas mediante la técnica de cubeta existen diferencias significativas en el recuento de S. mutans entre dientes con resina compuesta oclusal y dientes permanentes sanos, siendo mayor en las que presentaban resina compuesta.

Subjects/Keywords: Streptococcus mutans; Caries dental

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Sieber Carrasco, C. V. (2012). Recuento de Streptococcus mutans en muestras de biofilm sobre dientes restaurados con resina compuesta oclusal versus dientes sanos mediante el método de cubeta. (Thesis). Universidad de Chile. Retrieved from http://repositorio.uchile.cl/handle/2250/111754

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Sieber Carrasco, Carolina Verónica. “Recuento de Streptococcus mutans en muestras de biofilm sobre dientes restaurados con resina compuesta oclusal versus dientes sanos mediante el método de cubeta.” 2012. Thesis, Universidad de Chile. Accessed January 24, 2021. http://repositorio.uchile.cl/handle/2250/111754.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Sieber Carrasco, Carolina Verónica. “Recuento de Streptococcus mutans en muestras de biofilm sobre dientes restaurados con resina compuesta oclusal versus dientes sanos mediante el método de cubeta.” 2012. Web. 24 Jan 2021.

Vancouver:

Sieber Carrasco CV. Recuento de Streptococcus mutans en muestras de biofilm sobre dientes restaurados con resina compuesta oclusal versus dientes sanos mediante el método de cubeta. [Internet] [Thesis]. Universidad de Chile; 2012. [cited 2021 Jan 24]. Available from: http://repositorio.uchile.cl/handle/2250/111754.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Sieber Carrasco CV. Recuento de Streptococcus mutans en muestras de biofilm sobre dientes restaurados con resina compuesta oclusal versus dientes sanos mediante el método de cubeta. [Thesis]. Universidad de Chile; 2012. Available from: http://repositorio.uchile.cl/handle/2250/111754

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Manitoba

8. Lekic, Nikola. Effect of anticipatory guidance on the presence of cariogenic bacteria in preschool children.

Degree: Preventive Dental Science, 2014, University of Manitoba

URL: http://hdl.handle.net/1993/23810

► Objectives: This study was designed to address whether or not the level (high or low) of Streptococcus mutans in the saliva of preschool children can… (more)

▼ Objectives: This study was designed to address whether or not the level (high or low) of Streptococcus mutans in the saliva of preschool children can be used as an indicator of caries risk. Material and Methods: Levels of Streptococcus mutans was assessed in saliva of 100 preschool children, before and approximately 6-months after receiving a health promotion intervention known as anticipatory guidance. The S. mutans bacterial level was assessed using an immunoassay system (Saliva-Check MUTANS) based on monoclonal antibody technology. Bacterial counts were determined at baseline as well as at a six month recall examination. Obtained data was analyzed using SPSS 20.0 for Windows to report frequencies and to look for trends and associations between the dependent and independent variables. Bivariate analysis (ANOVA, Chi Square/Fishers/McNemar exact test) was used to identify associations between variables. Results: The main finding of this study was that higher levels of Streptococcus mutans were associated with higher caries prevalence at baseline, and caries incidence at follow-up. Furthermore following anticipatory guidance, the levels of Streptococcus mutans were reduced at the subsequent follow-up examination, approaching but not reaching statistical significance, and caries incidence was significantly lower in subjects demonstrating low Streptococcus mutans levels. Conclusions: Results from this study have shown that anticipatory guidance, offered to caregivers and children at an initial examination, led to a statistically non-significant reduction in the proportion of children with high Streptococcus mutans levels upon recall six mothers later. Children with low Streptococcus mutans levels were also less likely to develop new dental caries. These findings suggest that Streptococcus Mutans counts could be used to assess caries risk and encourages clinicians to provide anticipatory guidance to their patients to help avoid the development of new caries. Advisors/Committee Members: Brothwell, Doug (Preventive Dental Science) (supervisor), Cross, Howard (Preventive Dental Science) Cunha, Rodrigo (Restorative Dentistry) (examiningcommittee).

Subjects/Keywords: anticipatory guidance; Streptococcus mutans

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Lekic, N. (2014). Effect of anticipatory guidance on the presence of cariogenic bacteria in preschool children. (Masters Thesis). University of Manitoba. Retrieved from http://hdl.handle.net/1993/23810

Chicago Manual of Style (16^th Edition):

Lekic, Nikola. “Effect of anticipatory guidance on the presence of cariogenic bacteria in preschool children.” 2014. Masters Thesis, University of Manitoba. Accessed January 24, 2021. http://hdl.handle.net/1993/23810.

MLA Handbook (7^th Edition):

Lekic, Nikola. “Effect of anticipatory guidance on the presence of cariogenic bacteria in preschool children.” 2014. Web. 24 Jan 2021.

Vancouver:

Lekic N. Effect of anticipatory guidance on the presence of cariogenic bacteria in preschool children. [Internet] [Masters thesis]. University of Manitoba; 2014. [cited 2021 Jan 24]. Available from: http://hdl.handle.net/1993/23810.

Council of Science Editors:

Lekic N. Effect of anticipatory guidance on the presence of cariogenic bacteria in preschool children. [Masters Thesis]. University of Manitoba; 2014. Available from: http://hdl.handle.net/1993/23810

9. 眞弓, 佳代子. Antibacterial coating of human dentin surface with surface pre-reacted glassionomer (S-PRG) nanofillers.

Degree: 博士(歯学), 歯学, 2019, Hokkaido University

URL: http://hdl.handle.net/2115/77150

► Surface pre-reacted glass ionomer (S-PRG) filler shows tooth remineralization and antibacterial effects via ion-releasing. In this study, we fabricated the nano- and micro-sized S-PRG fillers… (more)

Subjects/Keywords: Actinomyces naeslundii; cytotoxicity; Streptococcus mutans

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APA (6^th Edition):

眞弓, �. (2019). Antibacterial coating of human dentin surface with surface pre-reacted glassionomer (S-PRG) nanofillers. (Doctoral Dissertation). Hokkaido University. Retrieved from http://hdl.handle.net/2115/77150

Chicago Manual of Style (16^th Edition):

眞弓, 佳代子. “Antibacterial coating of human dentin surface with surface pre-reacted glassionomer (S-PRG) nanofillers.” 2019. Doctoral Dissertation, Hokkaido University. Accessed January 24, 2021. http://hdl.handle.net/2115/77150.

MLA Handbook (7^th Edition):

眞弓, 佳代子. “Antibacterial coating of human dentin surface with surface pre-reacted glassionomer (S-PRG) nanofillers.” 2019. Web. 24 Jan 2021.

Vancouver:

眞弓 �. Antibacterial coating of human dentin surface with surface pre-reacted glassionomer (S-PRG) nanofillers. [Internet] [Doctoral dissertation]. Hokkaido University; 2019. [cited 2021 Jan 24]. Available from: http://hdl.handle.net/2115/77150.

Council of Science Editors:

眞弓 �. Antibacterial coating of human dentin surface with surface pre-reacted glassionomer (S-PRG) nanofillers. [Doctoral Dissertation]. Hokkaido University; 2019. Available from: http://hdl.handle.net/2115/77150

Universidad de Chile

10. Salinas Guerra, Ariel Andrés. Efecto del nivel de Streptococcus mutans salival, índice de higiene oral e índice de comportamiento en higiene oral sobre el índice CEOD en niños y niñas de 6 años beneficiarios del programa de salud oral integral en la Región Metropolitana.

Degree: 2013, Universidad de Chile

URL: http://repositorio.uchile.cl/handle/2250/117524

► Introducción Determinar el riesgo cariogénico del paciente es un requisito fundamental en la realización de un adecuado diagnóstico de salud bucal. Por esto es necesario… (more)

▼ Introducción Determinar el riesgo cariogénico del paciente es un requisito fundamental en la realización de un adecuado diagnóstico de salud bucal. Por esto es necesario analizar todos los factores involucrados en la enfermedad de caries, tanto los factores biológicos (como saliva, bacterias, historia de daño por caries) como psicológicos del individuo (Conductas en Higiene Oral). Conocer la relación de estas bacterias, conductas en Higiene Oral e Índice de Higiene Oral y cómo influencian en la prevalencia de caries, es un aspecto importante a considerar al desarrollar políticas públicas en salud oral, especialmente en el niño de 6 años, el cual constituye un grupo objetivo para el Estado, puesto que es a esa edad donde comienza a erupcionar la dentición definitiva. Materiales y Método Se seleccionaron 131 niños y niñas de 6 años beneficiarios del GES “Salud Oral Integral para niños y niñas de 6 años”en 2 consultorios de la Región Metropolitana. En este estudio se relacionó el Nivel de Streptococcus mutans, el Índice de Comportamiento en Higiene Oral, Índice de Higiene Oral e Índice ceod, con el fin de investigar cuál de los 3 primeros predice al último. El análisis estadístico realizado fue una regresión múltiple con el método de pasos sucesivos, aceptando un error estadístico tipo I (p<0,05) utilizando el software estadístico SPSS Stadistic. Los examinadores se sometieron a calibración interexaminador sobre el Índice de Higiene Oral. Los coeficientes Kappa demostraron una baja congruencia entre los examinadores (promedio 0,3), por lo que se eliminó del modelo estadístico. 5 Resultados El 43,5% (n=57) de los pacientes fueron mujeres y el 56,5% (n=74) hombres. El modelo mostró que el Nivel de S. mutans es predictor significativo del Índice ceod (F =11,422, p=0,001), explicando el 7,9% de la varianza, con una correlación positiva leve (0,294). Se excluye la variable Índice de Comportamiento en Higiene Oral debido a que tiene un p=0,420 (p>0,005). 1,121 Conclusiones El Nivel de S. mutans es predictor significativo del Índice ceod con una correlación positiva leve. El Nivel de S. mutans explica el 7,9% de la variación del Índice ceod. No existe correlación significativa entre el Índice de Comportamiento en Higiene oral y el Índice ceod.

Subjects/Keywords: Higiene bucal; Streptococcus mutans

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Salinas Guerra, A. A. (2013). Efecto del nivel de Streptococcus mutans salival, índice de higiene oral e índice de comportamiento en higiene oral sobre el índice CEOD en niños y niñas de 6 años beneficiarios del programa de salud oral integral en la Región Metropolitana. (Thesis). Universidad de Chile. Retrieved from http://repositorio.uchile.cl/handle/2250/117524

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Salinas Guerra, Ariel Andrés. “Efecto del nivel de Streptococcus mutans salival, índice de higiene oral e índice de comportamiento en higiene oral sobre el índice CEOD en niños y niñas de 6 años beneficiarios del programa de salud oral integral en la Región Metropolitana.” 2013. Thesis, Universidad de Chile. Accessed January 24, 2021. http://repositorio.uchile.cl/handle/2250/117524.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Salinas Guerra, Ariel Andrés. “Efecto del nivel de Streptococcus mutans salival, índice de higiene oral e índice de comportamiento en higiene oral sobre el índice CEOD en niños y niñas de 6 años beneficiarios del programa de salud oral integral en la Región Metropolitana.” 2013. Web. 24 Jan 2021.

Vancouver:

Salinas Guerra AA. Efecto del nivel de Streptococcus mutans salival, índice de higiene oral e índice de comportamiento en higiene oral sobre el índice CEOD en niños y niñas de 6 años beneficiarios del programa de salud oral integral en la Región Metropolitana. [Internet] [Thesis]. Universidad de Chile; 2013. [cited 2021 Jan 24]. Available from: http://repositorio.uchile.cl/handle/2250/117524.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Salinas Guerra AA. Efecto del nivel de Streptococcus mutans salival, índice de higiene oral e índice de comportamiento en higiene oral sobre el índice CEOD en niños y niñas de 6 años beneficiarios del programa de salud oral integral en la Región Metropolitana. [Thesis]. Universidad de Chile; 2013. Available from: http://repositorio.uchile.cl/handle/2250/117524

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Universidad de Chile

11. Hernández Parra, Dislie Tamara. Efecto remineralizante de un agente a base de fluor y grafeno, sobre discos de hidroxiapatita desmineralizada, con un modelo de biopelícula de Streptococcus mutans.

Degree: 2016, Universidad de Chile

URL: http://repositorio.uchile.cl/handle/2250/143094

► Introducción: La utilización de distintos modelos para generar desmineralización incipiente, ya sea en tejidos humanos o animales, es una práctica corriente para investigar los métodos… (more)

▼ Introducción: La utilización de distintos modelos para generar desmineralización incipiente, ya sea en tejidos humanos o animales, es una práctica corriente para investigar los métodos por los que se forma la lesión de caries y también para probar distintos compuestos farmacéuticos que la previenen y limitan. Unos de los métodos más conocidos y efectivos son los modelos que involucran especies bacterianas, ya que son representativos de lo que ocurre en la cavidad bucal. Objetivos: Demostrar que un agente a base de flúor y grafeno tiene acción remineralizante sobre zonas desmineralizadas de discos de hidroxiapatita, generadas por una biopelícula de Streptococcus mutans, a través de la medición de microdureza superficial. Metodología: Se utilizó un modelo desmineralizador de biopelícula monoespecie de dos cepas de S. mutans, ATCC 25175 y ATCC 35668, sobre discos de hidroxiapatita (HA) de manufactura propia. Éstos se expusieron a sacarosa al 10%, 4 veces al día por 5 minutos durante 7 días. Se determinó microdureza superficial de Vickers inicial en cada uno de los discos de HA previo a la exposición de la biopelícula monoespecie de S. mutans, después de exposición a biopelícula, y después de la aplicación del nuevo agente remineralizante. Resultados: Ambas cepas de S. mutans se adhirieron a los discos de HA de manufactura propia. Los valores de microdurezas de Vickers después de la exposición de los discos al modelo de biopelícula monoespecie de S. mutans fueron menores a los valores de microdureza superficial inicial. Los valores de dureza de Vickers después de la aplicación del nuevo agente remineralizador sobre la superficie desmineralizada de los discos de HA fue mayor que los valores de microdureza superficial desmineralizada, pero menor a los valores iniciales de dureza de los discos de HA. Conclusiones: el modelo in vitro de biopelícula monoespecie de S. mutans fue efectivo en la disminución de la microdureza superficial inicial de los discos de HA. El nuevo agente remineralizante aumentó los valores de microdureza superficial de los discos expuestos al modelo monoespecie de S. mutans.

Subjects/Keywords: Streptococcus mutans; Fluoruros tópicos

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Hernández Parra, D. T. (2016). Efecto remineralizante de un agente a base de fluor y grafeno, sobre discos de hidroxiapatita desmineralizada, con un modelo de biopelícula de Streptococcus mutans. (Thesis). Universidad de Chile. Retrieved from http://repositorio.uchile.cl/handle/2250/143094

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Hernández Parra, Dislie Tamara. “Efecto remineralizante de un agente a base de fluor y grafeno, sobre discos de hidroxiapatita desmineralizada, con un modelo de biopelícula de Streptococcus mutans.” 2016. Thesis, Universidad de Chile. Accessed January 24, 2021. http://repositorio.uchile.cl/handle/2250/143094.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Hernández Parra, Dislie Tamara. “Efecto remineralizante de un agente a base de fluor y grafeno, sobre discos de hidroxiapatita desmineralizada, con un modelo de biopelícula de Streptococcus mutans.” 2016. Web. 24 Jan 2021.

Vancouver:

Hernández Parra DT. Efecto remineralizante de un agente a base de fluor y grafeno, sobre discos de hidroxiapatita desmineralizada, con un modelo de biopelícula de Streptococcus mutans. [Internet] [Thesis]. Universidad de Chile; 2016. [cited 2021 Jan 24]. Available from: http://repositorio.uchile.cl/handle/2250/143094.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Hernández Parra DT. Efecto remineralizante de un agente a base de fluor y grafeno, sobre discos de hidroxiapatita desmineralizada, con un modelo de biopelícula de Streptococcus mutans. [Thesis]. Universidad de Chile; 2016. Available from: http://repositorio.uchile.cl/handle/2250/143094

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Universidad de Chile

12. Soler Guerra, Carlos. Preparación de adhesivos de resina modificado con nanopartículas de cobre y evaluación de sus propiedades antimicrobianas frente a Streptococcus mutans.

Degree: 2015, Universidad de Chile

URL: http://repositorio.uchile.cl/handle/2250/137350

► INTRODUCCIÓN: Las resinas compuestas dentales se están utilizando cada vez más como materiales de restauración. Sin embargo, un inconveniente importante de las resinas compuestas es… (more)

▼ INTRODUCCIÓN: Las resinas compuestas dentales se están utilizando cada vez más como materiales de restauración. Sin embargo, un inconveniente importante de las resinas compuestas es que tienden a acumular más biofilm y placa bacteriana que otros materiales de restauración. La caries adyacente a restauración es aquella que se detecta en los márgenes de una restauración existente, y es la razón más frecuente para reemplazar restauraciones. Diversos estudios muestran que Streptococcus mutans es la principal bacteria aislada de placa dental y con mayor potencial cariógenico. La incorporación de agentes antibacterianos en los materiales dentales puede inhibir la caries adyacente a restauración in vitro y reducir la formación de biofilm. A pesar de que existen diversas publicaciones científicas que demuestran las propiedades bactericidas de las nanopartículas de cobre (CuNP), su evaluación en adhesivos dentales ha sido poco explorada. MATERIAL Y METODOS: Se incorporaron CuNP a un adhesivo comercial (“Prime & Bond 2.1” Dentsply®). Se prepararon piezas cortadas de terceros molares con los adhesivos modificados y la versión comercial. El adhesivo modificado con CuNP se caracterizó mediante espectroscopia infrarroja de reflectancia total atenuada (FTIR-ART) y microscopia electrónica de barrido (SEM). La actividad antibacteriana se evaluó cuantificando formación de biofilm de Streptococcus mutans a través de tinción con cristal violeta, y por medio de recuento bacteriano. La liberación de cobre desde el adhesivo modificado con CuNP se evaluó en saliva artificial. RESULTADOS: Mediante la caracterización de las muestras se identificaron las CuNP incorporadas en el adhesivo y se comprobó que la estructura química del adhesivo no es alterada. En los ensayos de actividad antibacteriana, los adhesivos modificados con CuNP presentaron menor cantidad de biofilm y menor recuento bacteriano comparado con el control (98-99 % de inhibición de crecimiento bacteriano). Se comprobó que el adhesivo modificado presenta liberación de cobre al medio, lo cual podría explicar en parte su actividad antibacteriana. CONCLUSIÓN: El sistema adhesivo modificado con CuNP posee una marcada actividad antimicrobiana frente a Streptococcus mutans, afectando su adherencia y viabilidad. Este nuevo material modificado con CuNP podría ser una promisoria alternativa para la prevención de caries adyacentes a restauraciones.

Subjects/Keywords: Nanopartículas del metal; Streptococcus mutans

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APA (6^th Edition):

Soler Guerra, C. (2015). Preparación de adhesivos de resina modificado con nanopartículas de cobre y evaluación de sus propiedades antimicrobianas frente a Streptococcus mutans. (Thesis). Universidad de Chile. Retrieved from http://repositorio.uchile.cl/handle/2250/137350

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Soler Guerra, Carlos. “Preparación de adhesivos de resina modificado con nanopartículas de cobre y evaluación de sus propiedades antimicrobianas frente a Streptococcus mutans.” 2015. Thesis, Universidad de Chile. Accessed January 24, 2021. http://repositorio.uchile.cl/handle/2250/137350.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Soler Guerra, Carlos. “Preparación de adhesivos de resina modificado con nanopartículas de cobre y evaluación de sus propiedades antimicrobianas frente a Streptococcus mutans.” 2015. Web. 24 Jan 2021.

Vancouver:

Soler Guerra C. Preparación de adhesivos de resina modificado con nanopartículas de cobre y evaluación de sus propiedades antimicrobianas frente a Streptococcus mutans. [Internet] [Thesis]. Universidad de Chile; 2015. [cited 2021 Jan 24]. Available from: http://repositorio.uchile.cl/handle/2250/137350.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Soler Guerra C. Preparación de adhesivos de resina modificado con nanopartículas de cobre y evaluación de sus propiedades antimicrobianas frente a Streptococcus mutans. [Thesis]. Universidad de Chile; 2015. Available from: http://repositorio.uchile.cl/handle/2250/137350

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Universidad de Chile

13. Ciampi Díaz, Nicole. Efecto del tratamiento rehabilitador integral de caries temprana de la infancia en los niveles de Streptococcus mutans salivales de niños atendidos en la Clínica de Odontopediatría de la Escuela de Graduados de la Universidad de Chile.

Degree: 2013, Universidad de Chile

URL: http://repositorio.uchile.cl/handle/2250/117410

► Introducción: La caries temprana de la infancia (CTI) es una enfermedad dietobacteriana, siendo Streptococcus mutans (S.mutans) el principal patógeno asociado. Afecta a niños menores de… (more)

▼ Introducción: La caries temprana de la infancia (CTI) es una enfermedad dietobacteriana, siendo Streptococcus mutans (S.mutans) el principal patógeno asociado. Afecta a niños menores de 6 años, generando destrucción y dolor, siendo necesario un tratamiento que controle los factores de riesgo relacionados y recupere el tejido dañado. En Chile, no existen estudios que evalúen el impacto de un tratamiento rehabilitador integral en la microbiota oral de niños con CTI. Objetivo: Determinar el efecto del tratamiento rehabilitador integral de CTI en los niveles de Streptococcus mutans en niños tratados en la Clínica de Odontopediatría de la Escuela de Graduados de la Universidad de Chile Materiales y Métodos: Estudio retrospectivo observacional. Se analizaron 89 fichas clínicas de pacientes diagnosticados con CTI rehabilitados integralmente en el posgrado de Odontopediatría de la Universidad de Chile, las cuales registraban el recuento de S.mutans salival antes de iniciar el tratamiento, después del tratamiento preventivo y después del tratamiento rehabilitador. Se analizaron las diferencias entre los niveles de S.mutans de manera cuantitativa y según categorías (riesgo bajo, moderado o alto), durante las distintas etapas del tratamiento. Se analizaron también otras variables como tipo de material restaurador, edad e índice ceod. Resultados: Durante el análisis cuantitativo, se encontraron diferencias estadísticamente significativas al comparar las concentraciones de S.mutans salival inicial con las concentraciones luego del tratamiento preventivo (4,7±1,03 log UFC/ml vs 4,2 ± 0,96 log UFC/ml p<0,05) y luego del tratamiento rehabilitador (4,7±1,03 log UFC/ml vs 3,74 ± 0,88 log UFC/ml p<0,05), así como al comparar las concentraciones después del tratamiento preventivo con las posteriores al tratamiento rehabilitador (4,2 ± 0,96 log UFC/ml vs 3,74 ± 0,88 log UFC/ml p<0,05). Durante el análisis por categorías, sólo se encontraron diferencias significativas entre las concentraciones previas y después de ambos tratamientos, sin encontrar diferencias entre ambos tratamientos. No hubo diferencias al analizar las variaciones según categorías con el tipo de material restaurador, edad ni índice ceod. Conclusión: El tratamiento rehabilitador integral produce una reducción significativa en la concentración de S.mutans salival tanto en la etapa preventiva como en la etapa rehabilitadora.

Subjects/Keywords: Streptococcus mutans; Caries dental

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Ciampi Díaz, N. (2013). Efecto del tratamiento rehabilitador integral de caries temprana de la infancia en los niveles de Streptococcus mutans salivales de niños atendidos en la Clínica de Odontopediatría de la Escuela de Graduados de la Universidad de Chile. (Thesis). Universidad de Chile. Retrieved from http://repositorio.uchile.cl/handle/2250/117410

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Ciampi Díaz, Nicole. “Efecto del tratamiento rehabilitador integral de caries temprana de la infancia en los niveles de Streptococcus mutans salivales de niños atendidos en la Clínica de Odontopediatría de la Escuela de Graduados de la Universidad de Chile.” 2013. Thesis, Universidad de Chile. Accessed January 24, 2021. http://repositorio.uchile.cl/handle/2250/117410.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Ciampi Díaz, Nicole. “Efecto del tratamiento rehabilitador integral de caries temprana de la infancia en los niveles de Streptococcus mutans salivales de niños atendidos en la Clínica de Odontopediatría de la Escuela de Graduados de la Universidad de Chile.” 2013. Web. 24 Jan 2021.

Vancouver:

Ciampi Díaz N. Efecto del tratamiento rehabilitador integral de caries temprana de la infancia en los niveles de Streptococcus mutans salivales de niños atendidos en la Clínica de Odontopediatría de la Escuela de Graduados de la Universidad de Chile. [Internet] [Thesis]. Universidad de Chile; 2013. [cited 2021 Jan 24]. Available from: http://repositorio.uchile.cl/handle/2250/117410.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Ciampi Díaz N. Efecto del tratamiento rehabilitador integral de caries temprana de la infancia en los niveles de Streptococcus mutans salivales de niños atendidos en la Clínica de Odontopediatría de la Escuela de Graduados de la Universidad de Chile. [Thesis]. Universidad de Chile; 2013. Available from: http://repositorio.uchile.cl/handle/2250/117410

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Universidad de Chile

14. Duperat Sánchez, Lorena del Carmen. Implementación de metodología para la identificación y cuantificación de Streptococcus mutans mediante PCR en tiempo real en muestras de saliva y placa dental.

Degree: 2013, Universidad de Chile

URL: http://repositorio.uchile.cl/handle/2250/131302

► Son más de 700 especies bacterianas identificables en la cavidad oral humana. Entre ellas, Streptococcus mutans se cree que desempeña un papel importante como un… (more)

▼ Son más de 700 especies bacterianas identificables en la cavidad oral humana. Entre ellas, Streptococcus mutans se cree que desempeña un papel importante como un factor microbiológico para la iniciación de la caries dental. El manejo clínico de la caries dental se ha dirigido principalmente al tratamiento de las consecuencias del proceso de la enfermedad mediante restauraciones y no en su prevención. Con el uso de tecnología emergente, se podrá evaluar el riesgo de la enfermedad de caries en una fase anterior a la de la lesión incipiente de mancha blanca clínicamente visible. Recientemente se ha demostrado que PCR en tiempo real es un método sensible y rápido para la identificación y cuantificación de las especies microbianas individuales. Para este estudio se seleccionaron 27 niños al azar, de 8 años de edad, provenientes del área norte de la RM. Se recolectó 3ml. de saliva no estimulada, en las primeras horas de la mañana sin cepillado previo, solicitando su depósito en un tubo de plástico estéril, el cual se mantuvo a 4ºC hasta su traslado al laboratorio. La placa supragingival se recogió de todas las superficies dentarias lisas de dientes anteriores y posteriores con curetas periodontales estériles. Las muestras de saliva y placa bacteriana previamente rotuladas, se almacenaron a -80°C para su posterior procesamiento. Los partidores utilizados para cuantificar mediante qPCR el gen gtfB de S. mutans fueron Smut 3368-F y Smut 3481-R. Los resultados obtenidos de la amplificación mediante qPCR tuvo un porcentaje de eficiencia del 98% y el delta entre cada ciclo fue de 3,36. En la curva de melting se obtuvo un solo pick de amplificación a una misma temperatura para todas las muestras. La metodología presentada permite la identificación y cuantificación específica para el gen gtfB de S. mutans en muestras de saliva y biopelícula dental, de manera rápida y exacta. Lo que permite establecer el riesgo cariogénico individual de cada paciente y con ello realizar medidas preventivas, evitando así la aparición de lesiones y su posible transmisión.

Subjects/Keywords: Streptococcus mutans; Saliva-Microbiología

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Duperat Sánchez, L. d. C. (2013). Implementación de metodología para la identificación y cuantificación de Streptococcus mutans mediante PCR en tiempo real en muestras de saliva y placa dental. (Thesis). Universidad de Chile. Retrieved from http://repositorio.uchile.cl/handle/2250/131302

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Duperat Sánchez, Lorena del Carmen. “Implementación de metodología para la identificación y cuantificación de Streptococcus mutans mediante PCR en tiempo real en muestras de saliva y placa dental.” 2013. Thesis, Universidad de Chile. Accessed January 24, 2021. http://repositorio.uchile.cl/handle/2250/131302.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Duperat Sánchez, Lorena del Carmen. “Implementación de metodología para la identificación y cuantificación de Streptococcus mutans mediante PCR en tiempo real en muestras de saliva y placa dental.” 2013. Web. 24 Jan 2021.

Vancouver:

Duperat Sánchez LdC. Implementación de metodología para la identificación y cuantificación de Streptococcus mutans mediante PCR en tiempo real en muestras de saliva y placa dental. [Internet] [Thesis]. Universidad de Chile; 2013. [cited 2021 Jan 24]. Available from: http://repositorio.uchile.cl/handle/2250/131302.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Duperat Sánchez LdC. Implementación de metodología para la identificación y cuantificación de Streptococcus mutans mediante PCR en tiempo real en muestras de saliva y placa dental. [Thesis]. Universidad de Chile; 2013. Available from: http://repositorio.uchile.cl/handle/2250/131302

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Universidad de Chile

15. Trepiana Fica, Diego Andrés. Síntesis de suspensiones de nanopartículas de cobre y quitosano, y evaluación de sus propiedades antimicrobianas frente a Streptococcus mutans.

Degree: 2015, Universidad de Chile

URL: http://repositorio.uchile.cl/handle/2250/131959

► INTRODUCCIÓN: En el biofilm dental se encuentran diversas bacterias, de las cuales Streptococcus mutans (S. mutans) es considerada como una de las de mayor potencial… (more)

▼ INTRODUCCIÓN: En el biofilm dental se encuentran diversas bacterias, de las cuales Streptococcus mutans (S. mutans) es considerada como una de las de mayor potencial cariogénico. Existen diversos métodos para el control del biofilm, entre los que se encuentran los métodos químicos. Estos métodos se basan en el uso de sustancias antisépticas y/o antibióticas que son utilizados como colutorios orales y que permiten disminuir o retardar la formación de la placa bacteriana. En la actualidad no existe un agente químico antimicrobiano ideal. Es por esto que constantemente se están realizando cambios a los agentes terapéuticos actualmente conocidos, ya sea mejorando sus propiedades antimicrobianas, sus propiedades de adhesión (sustantividad) o disminuyendo sus efectos adversos. Es aquí donde la Nanotecnología y el desarrollo de nanomateriales han adquirido especial importancia debido a la posibilidad de sintetizar materiales con propiedades antimicrobianas. Es sabido que nanopartículas de quitosano (QuitNP) y nanopartículas de cobre (CuNP) presentan propiedades antimicrobianas frente a diversas bacterias, tanto Gram negativas como Gram positivas. Sin embargo no se ha probado la efectividad de las CuNP sobre S. mutans. Por otra parte estudios demuestran que nanopartículas metálicas, como el cobre, soportadas en una matriz de quitosano, presentarían mejoras en sus propiedades de adhesión. El objetivo de este trabajo de tesis es la síntesis de nanopatículas de cobre y quitosano, y evaluar sus propiedades antimicrobianas frente a S. mutans. MATERIAL Y METODOS: Se sintetizaron CuNP c/a utilizando almidón como agente reductor y estabilizante, CuNP s/a sin utilizar almidón, QuitNP y una nanopartícula hibrida (CuNP/QuitNP). Para esto se usaron reactivos biocompatibles, que permitieron la reducción a nanopartículas metálicas, y la gelificación iónica de nanopartículas poliméricas. Las muestras fueron caracterizadas mediante espectrofotometría de absorción molecular, microscopia electrónica de barrido (SEM), difracción de rayos X (DRX), espectroscopia infrarroja de reflectancia total atenuada (FTIR-ART) y análisis termogravimétricos (TGA). Se realizaron ensayos de actividad bactericida, para lo cual se determinó la concentración inhibitoria mínima (CIM) utilizando el método de incubación de medio de cultivo líquido, y la concentración bactericida mínima (CBM) realizando el recuento de unidades formadoras de colonias (UFC) en placas de agar BHI, para las nanopartículas sintetizadas. Además se realizaron pruebas de actividad bactericida sobre un biofilm formado en superficies dentarias. Para esto se utilizaron fragmentos de terceros molares, con un biofilm formado, los que fueron tratados con distintas soluciones antimicrobianas y suspensiones de nanopartículas. RESULTADOS: Utilizando el concepto de “química verde”, se sintetizaron y caracterizaron las partículas de carácter nanométrico; CuNP c/a, QuitNP y CuNP/QuitNP. Por otro lado, las partículas CuNP s/a presentaron dimensiones …

Subjects/Keywords: Nanopartículas del metal; Streptococcus mutans

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Trepiana Fica, D. A. (2015). Síntesis de suspensiones de nanopartículas de cobre y quitosano, y evaluación de sus propiedades antimicrobianas frente a Streptococcus mutans. (Thesis). Universidad de Chile. Retrieved from http://repositorio.uchile.cl/handle/2250/131959

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Trepiana Fica, Diego Andrés. “Síntesis de suspensiones de nanopartículas de cobre y quitosano, y evaluación de sus propiedades antimicrobianas frente a Streptococcus mutans.” 2015. Thesis, Universidad de Chile. Accessed January 24, 2021. http://repositorio.uchile.cl/handle/2250/131959.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Trepiana Fica, Diego Andrés. “Síntesis de suspensiones de nanopartículas de cobre y quitosano, y evaluación de sus propiedades antimicrobianas frente a Streptococcus mutans.” 2015. Web. 24 Jan 2021.

Vancouver:

Trepiana Fica DA. Síntesis de suspensiones de nanopartículas de cobre y quitosano, y evaluación de sus propiedades antimicrobianas frente a Streptococcus mutans. [Internet] [Thesis]. Universidad de Chile; 2015. [cited 2021 Jan 24]. Available from: http://repositorio.uchile.cl/handle/2250/131959.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Trepiana Fica DA. Síntesis de suspensiones de nanopartículas de cobre y quitosano, y evaluación de sus propiedades antimicrobianas frente a Streptococcus mutans. [Thesis]. Universidad de Chile; 2015. Available from: http://repositorio.uchile.cl/handle/2250/131959

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Universidad de Chile

16. Padilla Ruiz, María Montserrat. Efecto remineralizante de distintas concentraciones de un agente a base de fluor y grafeno en bloques de esmalte desmineralizado.

Degree: 2018, Universidad de Chile

URL: http://repositorio.uchile.cl/handle/2250/173375

► Introducción: La elevada prevalencia de la enfermedad de caries, el daño acumulado en la salud y en la calidad de vida de las personas, genera… (more)

▼ Introducción: La elevada prevalencia de la enfermedad de caries, el daño acumulado en la salud y en la calidad de vida de las personas, genera la necesidad de desarrollar medidas de control de la enfermedad y de prevención de la aparición de lesiones. El desequilibrio en el proceso de remineralización y desmineralización en la superficie dentaria provocado por una actividad acidogénica de la biopelícula dental provoca la pérdida de estructura dentaria y la consiguiente aparición de lesiones de caries. Los agentes remineralizantes a base de flúor actúan en las etapas iniciales de la enfermedad, previniendo y deteniendo la progresión de lesiones de caries incipientes. El propósito de este estudio fue evaluar la capacidad remineralizante de distintas concentraciones de BlueRemin®, un nuevo agente remineralizador a base de flúor y grafeno, con el fin de estudiar sus propiedades y futuras aplicaciones clínicas. Objetivo: Evaluar las diferencias de dureza superficial y densidad mineral en especímenes de esmalte desmineralizado en un modelo de caries microbiológico y sometidos a un protocolo de remineralización con BlueRemin® a distintas concentraciones. Metodología: Se simuló un ambiente cariogénico sobre bloques de esmalte humano en un modelo de biopelícula monoespecie de Streptococcus mutans in vitro. Se obtuvo zonas de desmineralización superficial compatibles con lesiones de caries no cavitadas que luego fueron sometidas a un protocolo de remineralización con BlueRemin® a distintas concentraciones. El contenido mineral de los bloques fue evaluado por microdueza de Vickers (Hv) y microtomografía computada previo, posterior a la formación de la biopelícula y después de la aplicación de BlueRemin®. Se utilizaron los porcentajes de recuperación de dureza superficial (%RDS) y de densidad mineral (%RDM) como indicadores de remineralización. Resultados: Considerando los resultados de dureza superficial y densidad mineral, se registró una diferencia estadísticamente significativa entre los valores de los bloques remineralizados con respecto a los desmineralizados para todas las concentraciones de BlueRemin® aplicadas (p < 0,05). No se encontraron diferencias estadísticamente significativas entre los porcentajes de recuperación de dureza superficial y de densidad mineral obtenidos con las concentraciones utilizadas. Conclusiones: BlueRemin® tiene capacidad remineralizante. Se evidenció una tendencia hacia una relación dosis dependiente de los porcentajes de remineralización obtenidos al aplicar BlueRemin®.

Subjects/Keywords: Streptococcus mutans; Fluoruros tópicos

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Padilla Ruiz, M. M. (2018). Efecto remineralizante de distintas concentraciones de un agente a base de fluor y grafeno en bloques de esmalte desmineralizado. (Thesis). Universidad de Chile. Retrieved from http://repositorio.uchile.cl/handle/2250/173375

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Padilla Ruiz, María Montserrat. “Efecto remineralizante de distintas concentraciones de un agente a base de fluor y grafeno en bloques de esmalte desmineralizado.” 2018. Thesis, Universidad de Chile. Accessed January 24, 2021. http://repositorio.uchile.cl/handle/2250/173375.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Padilla Ruiz, María Montserrat. “Efecto remineralizante de distintas concentraciones de un agente a base de fluor y grafeno en bloques de esmalte desmineralizado.” 2018. Web. 24 Jan 2021.

Vancouver:

Padilla Ruiz MM. Efecto remineralizante de distintas concentraciones de un agente a base de fluor y grafeno en bloques de esmalte desmineralizado. [Internet] [Thesis]. Universidad de Chile; 2018. [cited 2021 Jan 24]. Available from: http://repositorio.uchile.cl/handle/2250/173375.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Padilla Ruiz MM. Efecto remineralizante de distintas concentraciones de un agente a base de fluor y grafeno en bloques de esmalte desmineralizado. [Thesis]. Universidad de Chile; 2018. Available from: http://repositorio.uchile.cl/handle/2250/173375

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

17. Jorgiana Silva de Assis. Analysis of antimicrobial flavonoid epigallocatechin-3-gallate as a cavity cleansing agent in artificially carious dentin.

Degree: Master, 2013, Universidade Federal do Ceará

URL: http://www.teses.ufc.br/tde_busca/arquivo.php?codArquivo=11169 ;

►

The aim of this study was to evaluate the efficacy of the flavonoid epigallocatechin-3-gallate (EGCG) in concentrations of 0.5%, 1% and 2% as an antimicrobial… (more)

▼

The aim of this study was to evaluate the efficacy of the flavonoid epigallocatechin-3-gallate (EGCG) in concentrations of 0.5%, 1% and 2% as an antimicrobial solution in artificially carious dentin, with 2% chlorhexidine and 0.9% saline solution as controls. Twenty-five slabs of human dentin (4 mm x 4 mm) were immersed for five days in Brain Heart Infusion broth (BHI-broth) inoculated at first day with Streptococcus mutans UA159 (Batch Culture Model). On the fifth day of the experiment, the blocks were randomly divided into five groups: group I - negative control - 0.9% saline solution, group II - positive control - 2% chlorhexidine, group III - 0.5% EGCG, group IV - 1% EGCG, group V - 2% EGCG. Each slab was subjected to 15 Âl of the tested solution for 60 seconds. After treatments, artificially carious dentin was removed from the dentin slabs and analyzed by counting colony forming units (CFUs). All experiments were performed in triplicate and the data obtained in CFUs mean values converted to log base-10. The statistical tests used were analysis of variance (ANOVA) followed by Tukey test. There was no statistical difference between EGCG concentrations and saline (p > 0.05). Furthermore, there is no statistical difference between EGCG concentrations (p > 0.05). However, there was statistically significant difference between chlorhexidine and the other groups (p < 0.05). From the data obtained in this study, is possible to conclude that the substance tested is not effective on elimination of pathogen S. mutans when used in a concentration of 0.5%, 1% and 2% in artificially carious dentin.

O objetivo do presente estudo foi avaliar a eficÃcia do flavonÃide epigalocatequina-3-galato (EGCG) nas concentraÃÃes de 0,5%, 1% e 2% como soluÃÃo antimicrobiana em dentina artificialmente cariada, tendo a clorexidina 2% e a soluÃÃo salina 0,9% como controles. Vinte e cinco blocos de dentina humana (4 mm x 4 mm) foram imersos por cinco dias em BHI-caldo inoculado com Streptococcus mutans UA159 no primeiro dia do experimento. No quinto dia do experimento, os blocos foram aleatoriamente distribuÃdos em cinco grupos: grupo I â controle negativo â soluÃÃo salina 0,9%; grupo II â controle positivo â clorexidina 2%; grupo III â EGCG 0,5%; grupo IV â EGCG 1%; grupo V - EGCG 2%. Cada bloco recebeu tratamento de 15 Âl da soluÃÃo testada, que permaneceu em contato com o bloco por 60 segundos. ApÃs os tratamentos, amostras dentinÃrias foram removidas com emprego de lÃmina de bisturi e foram analisadas a partir da contagem de UFCs (unidades formadoras de colÃnias). Os experimentos foram realizados em triplicata e os dados, obtidos em UFCs foram convertidos em log base-10. Os testes estatÃsticos empregados foram anÃlise de variÃncia (ANOVA), seguida de Teste de Tukey. NÃo houve diferenÃa estatÃstica entre as concentraÃÃes de EGCG empregadas e a soluÃÃo salina (p > 0,05). AlÃm disso, nÃo houve diferenÃa estatÃstica entre as concentraÃÃes de EGCG (p > 0,05). No entanto, houve diferenÃa estatisticamente significativa entre a clorexidina e os…

Advisors/Committee Members: Cristiane SÃ Roriz Fonteles, SÃrgio Lima Santiago, ClÃudio Maniglia Ferreira.

Subjects/Keywords: ODONTOLOGIA; Catequina; Dentina; Streptococcus mutans; Catechin; Dentin; Streptococcus mutans

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Assis, J. S. d. (2013). Analysis of antimicrobial flavonoid epigallocatechin-3-gallate as a cavity cleansing agent in artificially carious dentin. (Masters Thesis). Universidade Federal do Ceará. Retrieved from http://www.teses.ufc.br/tde_busca/arquivo.php?codArquivo=11169 ;

Chicago Manual of Style (16^th Edition):

Assis, Jorgiana Silva de. “Analysis of antimicrobial flavonoid epigallocatechin-3-gallate as a cavity cleansing agent in artificially carious dentin.” 2013. Masters Thesis, Universidade Federal do Ceará. Accessed January 24, 2021. http://www.teses.ufc.br/tde_busca/arquivo.php?codArquivo=11169 ;.

MLA Handbook (7^th Edition):

Assis, Jorgiana Silva de. “Analysis of antimicrobial flavonoid epigallocatechin-3-gallate as a cavity cleansing agent in artificially carious dentin.” 2013. Web. 24 Jan 2021.

Vancouver:

Assis JSd. Analysis of antimicrobial flavonoid epigallocatechin-3-gallate as a cavity cleansing agent in artificially carious dentin. [Internet] [Masters thesis]. Universidade Federal do Ceará 2013. [cited 2021 Jan 24]. Available from: http://www.teses.ufc.br/tde_busca/arquivo.php?codArquivo=11169 ;.

Council of Science Editors:

Assis JSd. Analysis of antimicrobial flavonoid epigallocatechin-3-gallate as a cavity cleansing agent in artificially carious dentin. [Masters Thesis]. Universidade Federal do Ceará 2013. Available from: http://www.teses.ufc.br/tde_busca/arquivo.php?codArquivo=11169 ;

18. Denise Lins de Sousa. Efeito antibacteriano do acido anacÃrdico em culturas planctÃnicas e biofilmes de Streptococcus mutans.

Degree: PhD, 2014, Universidade Federal do Ceará

URL: http://www.teses.ufc.br/tde_busca/arquivo.php?codArquivo=11650 ;

►

Ãcido anacÃrdico Ã um composto extraÃdo do lÃquido da castanha de caju (LCC) e tem emergido como um composto promissor devido a sua variedade de… (more)

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Ãcido anacÃrdico Ã um composto extraÃdo do lÃquido da castanha de caju (LCC) e tem emergido como um composto promissor devido a sua variedade de propriedades biolÃgicas. Este estudo estÃ dividido em trÃs capÃtulos, cujos objetivos foram: capÃtulo 1) investigar a atividade antibacteriana de uma emulsÃo de Ãcidos anacÃrdicos, extraÃdos do LCC, e de uma emulsÃo sintÃtica do Ãcido anacÃrdico, em culturas planctÃnicas de Streptococcus mutans, bem como avaliar sua citotoxicidade in vitro; capÃtulo 2) avaliar o efeito de diferentes concentraÃÃes de uma emulsÃo de Ãcidos anacÃrdicos extraÃdos do LCC em biofilmes maduros de S. mutans; e capÃtulo 3) avaliar o efeito da aplicaÃÃo Ãnica versus aplicaÃÃo duas vezes ao dia de diferentes concentraÃÃes de uma emulsÃo de Ãcidos anacÃrdicos em biofilmes de S. mutans. A atividade antibacteriana das emulsÃes foi determinada atravÃs da concentraÃÃo inibitÃria mÃnima (CIM) e concentraÃÃo bactericida mÃnima (CBM), e a citotoxicidade foi mensurada atravÃs do reagente CellTiter BlueÂ (capÃtulo 1). Biofilmes foram crescidos em discos de hidroxiapatita imersos em caldo de peptona caseÃna soja e extrato de levedura com 1% sacarose por cinco dias. Biofilmes foram tratados com a emulsÃo de Ãcidos anacÃrdicos por um minuto no Ãltimo dia do experimento para avaliar seu efeito em biofilme maduro; viabilidade bacteriana e mensuraÃÃo de peso seco foram realizadas (capÃtulo 2). Diferentes concentraÃÃes da emulsÃo de Ãcidos anacÃrdicos foram aplicadas apenas no Ãltimo dia do experimento e duas vezes ao dia durante cinco dias para avaliar o efeito de diferentes aplicaÃÃes da emulsÃo de Ãcidos anacÃrdicos em biofilmes de S. mutans; viabilidade bacteriana, mensuraÃÃo de peso seco e quantificaÃÃo de polissacarÃdeos foram realizados (capÃtulo 3). A CIM e CBM da emulsÃo de Ãcidos anacÃrdicos (LCC) em cultura planctÃnica foram 0,48 μg/ml; e a CIM da emulsÃo sintÃtica do Ãcido anacÃrdico foi 4,38 μg/ml, mas sua CBM nÃo pÃde ser determinada (> 3.200 μg/ml) (capÃtulo 1). Observou-se uma reduÃÃo significante na viabilidade bacteriana de biofilmes maduros apÃs tratamento com as concentraÃÃes da emulsÃo de Ãcidos anacÃrdicos, mas estas nÃo alteraram o peso seco do biofilme (capÃtulo 2). O tratamento diÃrio com diferentes concentraÃÃes da emulsÃo de Ãcidos anacÃrdicos reduziu a viabilidade bacteriana do biofilme e modificou os nÃveis de polissacarÃdeos intracelular e extracelulares (capÃtulo 3). Pode-se concluir que a emulsÃo de Ãcidos anacÃrdicos apresenta-se como um promissor agente antibacteriano, tendo a capacidade de reduzir a viabilidade do S. mutans tanto em culturas planctÃnicas quanto em biofilmes.

Anacardic acid is an extract from processing of cashew nut shell liquid (CNSL) and it has been recognized to have several biological activities. This study is divided into three chapters, whose aims were: chapter 1) to investigate the antibacterial activity of an anacardic acids emulsion, from CNSL, and a synthetic emulsion of anacardic acid on planktonic cultures of S. mutans as well as to…

Advisors/Committee Members: Iriana Carla Junqueira Zanin, JosÃ JeovÃ Siebra Moreira Neto, Maria Izabel Florindo Guedes, Gislaine Cristina Padovani, Lidiany Karla Azevedo Rodrigues.

Subjects/Keywords: ODONTOLOGIA; Anacardium orientale; Streptococcus mutans; Biofilmes; Anacardium occidentale; Streptococcus mutans; biofilm

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Sousa, D. L. d. (2014). Efeito antibacteriano do acido anacÃrdico em culturas planctÃnicas e biofilmes de Streptococcus mutans. (Doctoral Dissertation). Universidade Federal do Ceará. Retrieved from http://www.teses.ufc.br/tde_busca/arquivo.php?codArquivo=11650 ;

Chicago Manual of Style (16^th Edition):

Sousa, Denise Lins de. “Efeito antibacteriano do acido anacÃrdico em culturas planctÃnicas e biofilmes de Streptococcus mutans.” 2014. Doctoral Dissertation, Universidade Federal do Ceará. Accessed January 24, 2021. http://www.teses.ufc.br/tde_busca/arquivo.php?codArquivo=11650 ;.

MLA Handbook (7^th Edition):

Sousa, Denise Lins de. “Efeito antibacteriano do acido anacÃrdico em culturas planctÃnicas e biofilmes de Streptococcus mutans.” 2014. Web. 24 Jan 2021.

Vancouver:

Sousa DLd. Efeito antibacteriano do acido anacÃrdico em culturas planctÃnicas e biofilmes de Streptococcus mutans. [Internet] [Doctoral dissertation]. Universidade Federal do Ceará 2014. [cited 2021 Jan 24]. Available from: http://www.teses.ufc.br/tde_busca/arquivo.php?codArquivo=11650 ;.

Council of Science Editors:

Sousa DLd. Efeito antibacteriano do acido anacÃrdico em culturas planctÃnicas e biofilmes de Streptococcus mutans. [Doctoral Dissertation]. Universidade Federal do Ceará 2014. Available from: http://www.teses.ufc.br/tde_busca/arquivo.php?codArquivo=11650 ;

19. Paschoal, Marco Aurélio Benini. Avaliação in vitro da terapia fotodinâmica sobre microrganismos cariogênicos presentes na saliva de crianças.

Degree: Mestrado, Odontopediatria, 2009, University of São Paulo

URL: http://www.teses.usp.br/teses/disponiveis/25/25133/tde-03072009-105829/ ;

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O surgimento de resistência bacteriana aos tratamentos convencionais tem proporcionado o desenvolvimento de novas modalidades terapêuticas para o tratamento e/ou controle da cárie dentária. Nesse… (more)

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O surgimento de resistência bacteriana aos tratamentos convencionais tem proporcionado o desenvolvimento de novas modalidades terapêuticas para o tratamento e/ou controle da cárie dentária. Nesse contexto, a utilização da terapia fotodinâmica (TFD) é sugerida como alternativa para a inativação de microrganismos patogênicos envolvidos na gênese da cárie. O objetivo do presente estudo foi avaliar in vitro o efeito antimicrobiano da terapia fotodinâmica (TFD) sobre três culturas de S. mutans: uma cepa padrão de S. mutans (ATCC 25175) e dois isolados clínicos (43513 e 47513) oriundos da saliva de crianças. O corante (C) azul de orto-toluidina (TBO) foi utilizado associado à iluminação com LEDs (L) no comprimento de onda vermelho. Estas suspensões foram transferidas para placas de 96 orifícios, tratadas com quatro concentrações de TBO (0,25; 2,5; 25 e 250 µg/mL) e expostas a quatro dosimetrias (12; 24; 36 e 48 J/cm2) constituindo o grupo C+L+ (TFD). Suspensões adicionais foram tratadas somente com as quatro concentrações de TBO (C+L-) ou apenas com as quatro dosimetrias (C-L+). Amostras não submetidas ao tratamento com a fonte de luz nem ao corante, constituíram a condição C-L- (controle positivo). Alíquotas de 100 µL de cada orifício foram transferidas para tubos de ensaio para se verificar a presença ou ausência de crescimento microbiológico. Adicionalmente, alíquotas de 25 µL do grupo correspondente a TFD (C+L+) foram semeadas em placas de Petri, as quais foram incubadas a 370C por 48 horas para posterior visualização de halos de inibição e/ou crescimento microbiológico correspondente a efetividade ou ineficiência da TFD, respectivamente. Com o intuito de confirmar os achados, essas mesmas amostras foram submetidas à análise pela microscopia confocal a laser. Os resultados demonstraram que a TFD, em determinadas condições experimentais, foi efetiva no controle do crescimento microbiológico das espécies de S. mutans usadas neste estudo. A concentração mínima de TBO necessária para a inativação in vitro das três culturas de S. mutans foi de 2,5 µg/mL associada à dosimetria mínima de 24 J/cm2 da fonte de luz LED utilizada no estudo.

The increase of bacterias resistance to conventional treatment resulted in the development of new therapeutic modalities for dental caries treatment and/or prevention. In this field, the use of photodynamic therapy (PDT) is suggested as an alternative for inactivation of patogenic microrganisms involved in the etiology of tooth decay. The aim of this study was to evaluate in vitro the antimicrobian effect of the photodynamic therapy (PDT) on bacteria suspensions of S. mutans (ATCC 25175) and two suspensions (43513 and 47513) from infants saliva. Toluidine blue O (TBO) (D) and a red light-emmiting diodes (LEDs) (L) were used in association. Samples were inserted into 96 well-plate and treated with four TBO concentrations (0.25; 2.5; 25 e 250 µg/mL) and exposed to four dosimetries (12; 24; 36 e 48 J/cm2) defining the D+L+ group (PDT). Additional samples were treated only with TBO (D+L-) or…

Advisors/Committee Members: Machado, Maria Aparecida de Andrade Moreira.

Subjects/Keywords: Streptococcus mutans; Bacteria; Bactéria; Photodynamic therapy; Streptococcus mutans; Terapia fotodinâmica

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Paschoal, M. A. B. (2009). Avaliação in vitro da terapia fotodinâmica sobre microrganismos cariogênicos presentes na saliva de crianças. (Masters Thesis). University of São Paulo. Retrieved from http://www.teses.usp.br/teses/disponiveis/25/25133/tde-03072009-105829/ ;

Chicago Manual of Style (16^th Edition):

Paschoal, Marco Aurélio Benini. “Avaliação in vitro da terapia fotodinâmica sobre microrganismos cariogênicos presentes na saliva de crianças.” 2009. Masters Thesis, University of São Paulo. Accessed January 24, 2021. http://www.teses.usp.br/teses/disponiveis/25/25133/tde-03072009-105829/ ;.

MLA Handbook (7^th Edition):

Paschoal, Marco Aurélio Benini. “Avaliação in vitro da terapia fotodinâmica sobre microrganismos cariogênicos presentes na saliva de crianças.” 2009. Web. 24 Jan 2021.

Vancouver:

Paschoal MAB. Avaliação in vitro da terapia fotodinâmica sobre microrganismos cariogênicos presentes na saliva de crianças. [Internet] [Masters thesis]. University of São Paulo; 2009. [cited 2021 Jan 24]. Available from: http://www.teses.usp.br/teses/disponiveis/25/25133/tde-03072009-105829/ ;.

Council of Science Editors:

Paschoal MAB. Avaliação in vitro da terapia fotodinâmica sobre microrganismos cariogênicos presentes na saliva de crianças. [Masters Thesis]. University of São Paulo; 2009. Available from: http://www.teses.usp.br/teses/disponiveis/25/25133/tde-03072009-105829/ ;

20. Bengtson, Camilla Regina Galvao. Estudo in vitro da influência da contaminação com Streptococcus mutans e da descontaminação com digluconato de clorexidina 2% na resistência de união de sistemas adesivos à dentina humana.

Degree: Mestrado, Dentística, 2007, University of São Paulo

URL: http://www.teses.usp.br/teses/disponiveis/23/23134/tde-13082007-155152/ ;

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O objetivo desse trabalho foi avaliar, in vitro, a influência da contaminação com Streptococcus mutans e da desinfecção com solução de digluconato de clorexidina 2%… (more)

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O objetivo desse trabalho foi avaliar, in vitro, a influência da contaminação com Streptococcus mutans e da desinfecção com solução de digluconato de clorexidina 2% na adesão de dois sistemas adesivos (um sistema adesivo com condicionamento ácido prévio e um sistema adesivo autocondicionante) à dentina de molares humanos. Para o estudo, foram utilizados 80 terceiros molares humanos hígidos, com a face oclusal lixada até a exposição de uma superfície plana de dentina. Os dentes foram divididos aleatoriamente em 8 grupos (n=10) de acordo com o sistema adesivo utilizado e tratamento superficial realizado: G1 ? Controle Single Bond 2 (SB); G2 ? Controle Clearfil SE Bond (SE); G3 ? Desinfecção com clorexidina SB; G4 ? Desinfecção com clorexidina SE; G5 ? Contaminação com Streptococcus mutans SB; G6 ? Contaminação com Streptococcus mutans SE; G7 ? Contaminação com Streptococcus mutans e desinfecção com clorexidina SB e G8 ? Contaminação com Streptococcus mutans e desinfecção com clorexidina SE. Corpos de prova em resina composta foram confeccionados nas superfícies tratadas e os dentes foram armazenados em água destilada à 37ºC por 24 horas. As amostras foram seccionadas verticalmente obtendo-se espécimes com área de secção transversal de aproximadamente 0,8mm2, que foram tracionados a velocidade de 0,5mm/min em máquina de ensaios universal. Cinco espécimes de cada grupo tiveram a interface adesiva analisada em microscopia eletrônica de varredura (MEV). Os resultados de resistência de união foram analisados usando os testes estatísticos ANOVA e Tukey (p<0,05). Os modos de fraturas foram avaliados por meio de lupa esteroscópica (25X). Discos de dentina foram obtidos de 6 dentes adicionais, para observação em MEV das superfícies submetidos aos mesmos tratamentos realizados para o teste de microtração. Não foram encontradas diferenças estatísticas entre os grupos controles (G1 = 31,52 ± 6,24 e G2 = 42,88 ± 2,31), o tratados com clorexidina (G3 = 34,41 ± 6,92 e G4 = 40,14 ± 2,91) e os grupos que foram submetidos à contaminação com Streptococcus mutans e descontaminação com clorexidina (G7 = 31,12 ± 5,31 e G8 = 39,09 ± 3,86). Os grupos submetidos à contaminação com Streptococcus mutans que não foram descontaminados antes da aplicação dos sistemas adesivos tiveram resultados significantemente menores que os grupos estéreis e descontaminados (G5 = 23,61 ± 7,13 e G6 = 33,15 ± 3,35). O sistema adesivo Clearfil SE Bond apresentou valores de resistência de união significantemente maiores que o sistema adesivo Single Bond 2, independente do tratamento da superficial dentina. Pôde-se concluir que a contaminação in vitro com Streptococcus mutans comprometeu a adesão dos dois sistemas adesivos e a solução de digluconato de clorexidina 2% foi capaz de eliminar a contaminação, restabelecendo os valores de resistência de união obtidos com o substrato estéril.

The purpose of this study was to evaluate, in vitro, the influence of Streptococcus mutans contamination and 2% chlorhexidine digluconate disinfection on the bond strength of two…

Advisors/Committee Members: Turbino, Miriam Lacalle.

Subjects/Keywords: Adesivos Dentinários; Chlorhexidine; Clorexidina; Dentin-Bonding Agents; Streptoccocus mutans; Streptoccocus mutans

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Bengtson, C. R. G. (2007). Estudo in vitro da influência da contaminação com Streptococcus mutans e da descontaminação com digluconato de clorexidina 2% na resistência de união de sistemas adesivos à dentina humana. (Masters Thesis). University of São Paulo. Retrieved from http://www.teses.usp.br/teses/disponiveis/23/23134/tde-13082007-155152/ ;

Chicago Manual of Style (16^th Edition):

Bengtson, Camilla Regina Galvao. “Estudo in vitro da influência da contaminação com Streptococcus mutans e da descontaminação com digluconato de clorexidina 2% na resistência de união de sistemas adesivos à dentina humana.” 2007. Masters Thesis, University of São Paulo. Accessed January 24, 2021. http://www.teses.usp.br/teses/disponiveis/23/23134/tde-13082007-155152/ ;.

MLA Handbook (7^th Edition):

Bengtson, Camilla Regina Galvao. “Estudo in vitro da influência da contaminação com Streptococcus mutans e da descontaminação com digluconato de clorexidina 2% na resistência de união de sistemas adesivos à dentina humana.” 2007. Web. 24 Jan 2021.

Vancouver:

Bengtson CRG. Estudo in vitro da influência da contaminação com Streptococcus mutans e da descontaminação com digluconato de clorexidina 2% na resistência de união de sistemas adesivos à dentina humana. [Internet] [Masters thesis]. University of São Paulo; 2007. [cited 2021 Jan 24]. Available from: http://www.teses.usp.br/teses/disponiveis/23/23134/tde-13082007-155152/ ;.

Council of Science Editors:

Bengtson CRG. Estudo in vitro da influência da contaminação com Streptococcus mutans e da descontaminação com digluconato de clorexidina 2% na resistência de união de sistemas adesivos à dentina humana. [Masters Thesis]. University of São Paulo; 2007. Available from: http://www.teses.usp.br/teses/disponiveis/23/23134/tde-13082007-155152/ ;

Universidade Estadual de Campinas

21. Costa, Ana Rosa, 1977-. Estudo in vitro do efeito da clorexidina e do etanol na dentina em diferentes condições do substrato dentinário e tempos de armazenagem : Study in vitro the effect of chlorhexidine and ethanol on dentin in different conditions of the dentin substrate and storage times: Study in vitro the effect of chlorhexidine and ethanol on dentin in different conditions of the dentin substrate and storage times.

Degree: 2013, Universidade Estadual de Campinas

URL: http://repositorio.unicamp.br/jspui/handle/REPOSIP/288584

► Abstract: The aim of this study was to evaluate the effect of 2% chlorhexidine digluconate (CHX) and 100% ethanol wet-bonding (Et) on the degradation of… (more)

▼ Abstract: The aim of this study was to evaluate the effect of 2% chlorhexidine digluconate (CHX) and 100% ethanol wet-bonding (Et) on the degradation of the adhesive system bond strength in sound or artificial caries-affected/infected dentin after different storage times under microtensile test (_TBS). The sound teeth were divided into 3 groups, according to dentin substrates: sound dentin, caries-infected dentin (maintained of caries) and caries-affected dentin (partial removal of caries). Non-carious human third molars had their occlusal enamel removed with a slow speed diamond saw, under copious water-cooling to expose a flat-surfaced sound dentin, which was submitted to the microbiological challenge (S. mutans) for the development of artificial caries. Spherical drill was used to remove soft pigmented carious tissue till hard and slightly pigmented dentin remains (Ca). After, the teeth will be assigned into 3 subgroups according to surface treatment: water wet- bonding (Ct) (Chapter 1); Ct and 100% ethanol application (Et) (Chapter 2); and, Ct and chlorhexidine digluconate 2% application (CHX) (Chapter 3). The substrate will was etched with 35% phosphoric acid gel for 15 s, rinsed for 30 s with tap water and dried with oil/water-free air. The CHX or Et was applied for 60 s, just after etching with 35% phosphoric acid gel. The adhesive system Adper Single Bond 2 (3M ESPE) was applied according to the manufacturer's instruction and polymerized for 10 s by a light-curing unit XL 2500. The bonded surfaces were coupled with a composite resin Filtek Z350 (3M ESPE) applied in 2 mm increments and polymerized using a curing unit XL 2500 (3M ESPE) for 40 s for each increment and to build 6 mm thick block. The restored teeth were stored in distilled water at 37o C for 24 h. After this period, the restored teeth were longitudinally sectioned across the bonded interface to produce a series of 1.0mm2 beams. The specimens were then submitted to three storage periods: 24 hours, 6 months, or 1 year. Afterwards, the storage periods, the specimens were submitted to a microtensile bond strength (_TBS) using EZ test machine (EZS, Shimadzu) at a crosshead speed of 0.5 mm/min until failure. Data were submitted to ANOVA and Tukey's HSD test (?=0.05). The bonding with sound dentin showed _TBS values significant higher when compared to caries-affected and caries-infected dentin. The 6 months and 1 year storage periods resulted in decreased bond strengths for all dentin conditions and/or treatment. The ethanol was not effective to improve the _TBS for the three dentin substrates evaluated. The CHX did not affect the 24- hour, 6 and 12 months _TBS, independent of the tested dentin substrates Advisors/Committee Members: UNIVERSIDADE ESTADUAL DE CAMPINAS (CRUESP), Puppin-Rontani, Regina Maria, 1959- (advisor), Universidade Estadual de Campinas. Faculdade de Odontologia de Piracicaba (institution), Programa de Pós-Graduação em Materiais Dentários (nameofprogram), Borges, Gilberto Antonio (committee member), Neto, Manoel Damião de Sousa (committee member), Consani, Simonides (committee member), Soares, Carlos José (committee member).

Subjects/Keywords: Streptococcus mutans; Adesivos dentinários; Streptococcus mutans; Dentin-bonding agents

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Costa, Ana Rosa, 1. (2013). Estudo in vitro do efeito da clorexidina e do etanol na dentina em diferentes condições do substrato dentinário e tempos de armazenagem : Study in vitro the effect of chlorhexidine and ethanol on dentin in different conditions of the dentin substrate and storage times: Study in vitro the effect of chlorhexidine and ethanol on dentin in different conditions of the dentin substrate and storage times. (Thesis). Universidade Estadual de Campinas. Retrieved from http://repositorio.unicamp.br/jspui/handle/REPOSIP/288584

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Costa, Ana Rosa, 1977-. “Estudo in vitro do efeito da clorexidina e do etanol na dentina em diferentes condições do substrato dentinário e tempos de armazenagem : Study in vitro the effect of chlorhexidine and ethanol on dentin in different conditions of the dentin substrate and storage times: Study in vitro the effect of chlorhexidine and ethanol on dentin in different conditions of the dentin substrate and storage times.” 2013. Thesis, Universidade Estadual de Campinas. Accessed January 24, 2021. http://repositorio.unicamp.br/jspui/handle/REPOSIP/288584.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Costa, Ana Rosa, 1977-. “Estudo in vitro do efeito da clorexidina e do etanol na dentina em diferentes condições do substrato dentinário e tempos de armazenagem : Study in vitro the effect of chlorhexidine and ethanol on dentin in different conditions of the dentin substrate and storage times: Study in vitro the effect of chlorhexidine and ethanol on dentin in different conditions of the dentin substrate and storage times.” 2013. Web. 24 Jan 2021.

Vancouver:

Costa, Ana Rosa 1. Estudo in vitro do efeito da clorexidina e do etanol na dentina em diferentes condições do substrato dentinário e tempos de armazenagem : Study in vitro the effect of chlorhexidine and ethanol on dentin in different conditions of the dentin substrate and storage times: Study in vitro the effect of chlorhexidine and ethanol on dentin in different conditions of the dentin substrate and storage times. [Internet] [Thesis]. Universidade Estadual de Campinas; 2013. [cited 2021 Jan 24]. Available from: http://repositorio.unicamp.br/jspui/handle/REPOSIP/288584.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Costa, Ana Rosa 1. Estudo in vitro do efeito da clorexidina e do etanol na dentina em diferentes condições do substrato dentinário e tempos de armazenagem : Study in vitro the effect of chlorhexidine and ethanol on dentin in different conditions of the dentin substrate and storage times: Study in vitro the effect of chlorhexidine and ethanol on dentin in different conditions of the dentin substrate and storage times. [Thesis]. Universidade Estadual de Campinas; 2013. Available from: http://repositorio.unicamp.br/jspui/handle/REPOSIP/288584

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Toronto

22. Bottner, Aaron. Genotyping and Phenotypic Analysis of Streptococcus mutans Isolates From Children With and Without Dental Caries.

Degree: 2018, University of Toronto

URL: http://hdl.handle.net/1807/91440

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Within dental plaque, Streptococcus mutans produces acids from fermentable carbohydrates, decreasing the biofilm pH, causing enamel demineralization. S. mutans produces persisters, dormant variants of cells… (more)

Subjects/Keywords: Acidurance; Caries; Early Childhood Caries; Persistence; S mutans; Streptococcus mutans; 0410

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Bottner, A. (2018). Genotyping and Phenotypic Analysis of Streptococcus mutans Isolates From Children With and Without Dental Caries. (Masters Thesis). University of Toronto. Retrieved from http://hdl.handle.net/1807/91440

Chicago Manual of Style (16^th Edition):

Bottner, Aaron. “Genotyping and Phenotypic Analysis of Streptococcus mutans Isolates From Children With and Without Dental Caries.” 2018. Masters Thesis, University of Toronto. Accessed January 24, 2021. http://hdl.handle.net/1807/91440.

MLA Handbook (7^th Edition):

Bottner, Aaron. “Genotyping and Phenotypic Analysis of Streptococcus mutans Isolates From Children With and Without Dental Caries.” 2018. Web. 24 Jan 2021.

Vancouver:

Bottner A. Genotyping and Phenotypic Analysis of Streptococcus mutans Isolates From Children With and Without Dental Caries. [Internet] [Masters thesis]. University of Toronto; 2018. [cited 2021 Jan 24]. Available from: http://hdl.handle.net/1807/91440.

Council of Science Editors:

Bottner A. Genotyping and Phenotypic Analysis of Streptococcus mutans Isolates From Children With and Without Dental Caries. [Masters Thesis]. University of Toronto; 2018. Available from: http://hdl.handle.net/1807/91440

IUPUI

23. El-ezmerli, Nasreen Farouk. Effect of nicotine on biofilm formation of streptococous mutans isolates from smoking versus non-smoking human subjects.

Degree: 2017, IUPUI

URL: http://hdl.handle.net/1805/13992

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Indiana University-Purdue University Indianapolis (IUPUI)

Tooth decay is a complex dieto-bacterial disease with an association of social, behavioral and biological factors. Streptococcus mutans plays a… (more)

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Indiana University-Purdue University Indianapolis (IUPUI)

Tooth decay is a complex dieto-bacterial disease with an association of social, behavioral and biological factors. Streptococcus mutans plays a major role in tooth decay. This endogenous oral microorganism adheres to tooth surfaces and grows and develops into micro-communities that mature and form dental biofilm. Development of cariogenic biofilm is one of the major factors associated with the tooth decay process. The use of tobacco is considered a great risk factor for oral diseases. Several studies demonstrated the association of tooth decay and the use of tobacco as effects of first-hand or second- hand smoking. Nicotine has been reported to increase the biofilm growth and metabolism of S. mutans in a dose-dependent manner up to 16 mg/ml of nicotine. However, its effects on biofilm formation of S. mutans strains isolated from smokers are not known and should be investigated. Therefore, we proposed the use of an in-vitro model to better understand the effects of nicotine on biofilm formation of strains of S. mutans isolates from smokers versus non-smoking subjects. Objectives: To investigate the effects of nicotine on biofilm formation of S. mutans isolates from oral washes of smoker and non-smoker human subjects. Materials and Methods: This study was conducted using three S. mutans isolates collected from oral washes of 10 smoking subjects and 10 non-smoking subjects. The oral wash samples were stored at -80 °C before S. mutans isolation. S. mutans isolates were obtained by plating on Mitis Salivarius Sucrose Bacitracin plates and species identity confirmed by carbohydrate fermentation assays. Nicotine from Sigma-Aldrich (St. Louis, MO, USA) was used. Biofilm was formed by overnight culturing of each S. mutans strain (10 μl) in 190 μl of tryptic soy broth (TSB) supplemented with 1.0-percent sucrose (TSBS) containing 0 mg/ml, 0.25 mg/ml, 0.5 mg/ml, 1.0 mg/ml, 2.0 mg/ml, 4.0 mg/ml, 8.0 mg/ml, 16.0 mg/ml, and 32.0 mg/ml of nicotine for 24 hours in 5.0-percent CO2 at 37 °C in sterile (8 x 12) 96-well microtiter plates (Fisher Scientific, Newark, DE, USA). The absorbance values of biofilm were measured at 490 nm in a microplate spectrophotometer (SpectraMax 190; Molecular Devices, SunnyVale, CA, USA) after crystal violet staining. Null Hypotheses: 1) Nicotine will not increase biofilm formation in both smoker and non-smoker S. mutans isolates. 2) An increase in nicotine concentrations will not increase biofilm formation in both smoker and non-smoker S. mutans isolates in a dose-dependent manner. 3) Nicotine will not produce significant differences in biofilm formation between smoker and non-smoker S. mutans isolates. Alternative Hypotheses: 1) Nicotine increases the growth of biofilm formation in both smoker and non-smoker S. mutans isolates. 2) An increase in nicotine concentrations increase biofilm formation of both smoker and non-smoker S. mutans isolates in a dose-dependent manner. 3) However, nicotine increases biofilm formation of smoker S. mutans…

Advisors/Committee Members: Gregory, Richard L., Hara, Anderson T., Cook, Norman Blaine, Windsor, Jack L..

Subjects/Keywords: Smoking; Biofilm; Streptococous Mutans; Smoking; Biofilms; Streptococcus mutans; Nicotine

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

El-ezmerli, N. F. (2017). Effect of nicotine on biofilm formation of streptococous mutans isolates from smoking versus non-smoking human subjects. (Thesis). IUPUI. Retrieved from http://hdl.handle.net/1805/13992

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

El-ezmerli, Nasreen Farouk. “Effect of nicotine on biofilm formation of streptococous mutans isolates from smoking versus non-smoking human subjects.” 2017. Thesis, IUPUI. Accessed January 24, 2021. http://hdl.handle.net/1805/13992.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

El-ezmerli, Nasreen Farouk. “Effect of nicotine on biofilm formation of streptococous mutans isolates from smoking versus non-smoking human subjects.” 2017. Web. 24 Jan 2021.

Vancouver:

El-ezmerli NF. Effect of nicotine on biofilm formation of streptococous mutans isolates from smoking versus non-smoking human subjects. [Internet] [Thesis]. IUPUI; 2017. [cited 2021 Jan 24]. Available from: http://hdl.handle.net/1805/13992.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

El-ezmerli NF. Effect of nicotine on biofilm formation of streptococous mutans isolates from smoking versus non-smoking human subjects. [Thesis]. IUPUI; 2017. Available from: http://hdl.handle.net/1805/13992

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

24. Bruno Rocha da Silva. Atividade antimicrobiana do peptÃdeo sintÃtico LYS-A1 frente a estreptococos orais.

Degree: Master, 2013, Universidade Federal do Ceará

URL: http://www.teses.ufc.br/tde_busca/arquivo.php?codArquivo=9436 ;

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A cÃrie dental Ã conceituada como uma doenÃa infectocontagiosa, crÃnica e multifatorial na qual ocorre uma desmineralizaÃÃo progressiva das estruturas dentais com consequente dor e… (more)

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A cÃrie dental Ã conceituada como uma doenÃa infectocontagiosa, crÃnica e multifatorial na qual ocorre uma desmineralizaÃÃo progressiva das estruturas dentais com consequente dor e perda do elemento dental. Ã considerada um problema de saÃde pÃblica em todo mundo devido sua incidÃncia e consequÃncias orais e sistÃmicas. Dessa forma, novos mÃtodos de controle microbiano tÃm sido pesquisados com vista Ã reduÃÃo do nÃmero de casos. Os peptÃdeos antimicrobianos sÃo molÃculas presentes em diversos seres vivos e possuem uma alta atividade biocida frente a diversos microrganismos patogÃnicos. O peptÃdeo Lys-[Trp6]-Hy-A1 (Lys-a1), derivado sintÃtico do peptÃdeo Hy-A1, isolado inicialmente da espÃcie Hypsiboas albopunctatus, Ã uma molÃcula com atividade antimicrobiana descrita na literatura. Dessa forma, o objetivo deste trabalho foi avaliar o potencial antibacteriano do peptÃdeo sintÃtico Lys-a1 sobre crescimento planctÃnico e em biofilme de bactÃrias orais. As metodologias utilizadas para avaliaÃÃo do potencial antimicrobiano foram: determinaÃÃo da concentraÃÃo inibitÃria mÃnima (CIM) e concentraÃÃo bactericida mÃnima (CBM) em placas de poliestireno para o crescimento em suspensÃo; e quantificaÃÃo de biomassa por cristal violeta e contagem de unidades formadoras para crescimento em biofilme. Os micro-organismos, S. oralis, S. sanguinis, S. parasanguinis, S. salivarius, S. mutans e S. sobrinus, foram cultivados em Brain Heart Inffusion caldo suplementado com 1% de sacarose (BHIs) a 37 ÂC sob atmosfera com 10% de CO2. O peptÃdeo foi solubilizado em Ãcido acÃtico 0,1% (v/v) em diferentes concentraÃÃes (500 a 1,9 Âg.mL-1). Os grupos controle dos ensaios foram meio de cultura BHIs (controle negativo) e Gluconato de Clorexidina 0,12% (controle positivo). O peptÃdeo testado apresentou um destacado efeito antimicrobiano, sendo capaz de inibir o crescimento planctÃnico e em biofilme de todas as cepas testadas mesmo em baixas concentraÃÃes. Assim, o peptÃdeo Lys-a1 Ã uma importante fonte para possÃveis agentes antimicrobianos, com Ãnfase no controle e prevenÃÃo de biofilmes microbianos, um dos fatores mais importantes para o desenvolvimento do processo cariogÃnico.

Dental caries is defined as an infectious, chronic and multifactorial disease, in which there is a progressive demineralization of tooth structure with consequent pain and dental loss. It is considered a major public health problem worldwide because its high incidence, besides its oral and systemic consequences. Thus, new methods of microbial control have been investigated to reduce the number of cases. Antimicrobial peptides are molecules present in many living beings and have a high biocidal activity against various pathogenic microrganisms. The peptide Lys-[Trp6]-Hy-A1 (Lys-a1) is a synthetic derivative of the peptide Hy-A1, initially isolated from the species Hypsiboas albopunctatus. According to previous research, it is a molecule with broad antimicrobial activity. The objective of this study was to evaluate the antimicrobial activity of the synthetic peptide…

Advisors/Committee Members: Victor Alves Carneiro, Edson Holanda Teixeira, Eduardo Maffud Cilli.

Subjects/Keywords: PeptÃdeos Antimicrobianos; MICROBIOLOGIA MEDICA; Biofilmes; CÃrie Dental; Streptococcus mutans; Antimicrobial peptide; Biofilms; Streptococcus mutans; Dental caries; Streptococus mutans; CÃrie dentÃria

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Silva, B. R. d. (2013). Atividade antimicrobiana do peptÃdeo sintÃtico LYS-A1 frente a estreptococos orais. (Masters Thesis). Universidade Federal do Ceará. Retrieved from http://www.teses.ufc.br/tde_busca/arquivo.php?codArquivo=9436 ;

Chicago Manual of Style (16^th Edition):

Silva, Bruno Rocha da. “Atividade antimicrobiana do peptÃdeo sintÃtico LYS-A1 frente a estreptococos orais.” 2013. Masters Thesis, Universidade Federal do Ceará. Accessed January 24, 2021. http://www.teses.ufc.br/tde_busca/arquivo.php?codArquivo=9436 ;.

MLA Handbook (7^th Edition):

Silva, Bruno Rocha da. “Atividade antimicrobiana do peptÃdeo sintÃtico LYS-A1 frente a estreptococos orais.” 2013. Web. 24 Jan 2021.

Vancouver:

Silva BRd. Atividade antimicrobiana do peptÃdeo sintÃtico LYS-A1 frente a estreptococos orais. [Internet] [Masters thesis]. Universidade Federal do Ceará 2013. [cited 2021 Jan 24]. Available from: http://www.teses.ufc.br/tde_busca/arquivo.php?codArquivo=9436 ;.

Council of Science Editors:

Silva BRd. Atividade antimicrobiana do peptÃdeo sintÃtico LYS-A1 frente a estreptococos orais. [Masters Thesis]. Universidade Federal do Ceará 2013. Available from: http://www.teses.ufc.br/tde_busca/arquivo.php?codArquivo=9436 ;

25. Rafaela Mesquita Bastos. AvaliaÃÃo da atividade antimicrobiana dos compostos isolados da planta Mitracarpus Baturitensis frente Ã Streptococcus Mutans.

Degree: Master, 2013, Universidade Federal do Ceará

URL: http://www.teses.ufc.br/tde_busca/arquivo.php?codArquivo=13019 ;

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A Mitracapus (Rubiacea) Ã um gÃnero neotropical, com trÃs centros de diversidades no MÃxico, Brasil e Caribe. Em relaÃÃo Ã Mitracarpus baturitensis, ainda nÃo existe… (more)

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A Mitracapus (Rubiacea) Ã um gÃnero neotropical, com trÃs centros de diversidades no MÃxico, Brasil e Caribe. Em relaÃÃo Ã Mitracarpus baturitensis, ainda nÃo existe relatos na lieratura de estudos fitoquÃmicos e biolÃgicos, devido Ã mesma ser uma espÃcie recÃm-descoberta. Portanto o objetivo deste estudo foi avaliar a atividade antimicrobiana dos compostos obtidos do extrato etanÃlico das partes aÃreas de Mitracarpus baturitensis contra a bactÃria Streptococcus mutans. Os triterpenos Ãcido 3β-hidroxi-urs-12-en-28-oico (Ãcido ursÃlico â AU) e o Ãcido 28- O-β-D-glucopiranosil-ester (Ãcido pomÃlico) foram isolados do extrato etanÃlico empregando tÃcnicas cromatogrÃficas usuais como: cromatografia gravitacional em gel de sÃlica, cromatografia flesh e cromatogrÃfia de camada delgada. A caracterizaÃÃo estrutural destes compostos foi realizado pelo uso de RNMâH e 13C, alÃm de comparar mÃtodos espectroscÃpico IV, reaÃÃes com base na literatura. ApÃs a identificaÃÃo foram realizados teste de ConcentraÃÃo InibitÃria MÃnima (CIM), ConcentraÃÃo Bactericida MÃnima (CBM), Curva do Tempo de Morte, aÃÃo antibiofilme e ensaio de ataque ao biofilme. O Ãcido ursÃlico inibiu o crescimento bacteriano nas concentraÃÃes de 500-62,5 μg. mL-1 sendo bactericida na concentraÃÃo de 500 μg. mL-1. AlÃm disso, o mesmo teve aÃÃo sobre a morte de S. mutans em apenas 30 minutos de contato. Ãcido ursÃlico tambÃm apresentou efeito sobre a formaÃÃo do biofilme reduzindo a biomassa em 97,37%, nÃo obtendo o mesmo resultado sobre o biofilme formado por 24 horas. NÃo foram obtidos resultados semelhantes para o Ãcido pomÃlico. Assim, Ãcido ursÃlico pode ser considerado como um candidato Ã insumo biotecnolÃgico com impacto na pesquisa de antimicrobianos.

The Mitracapus (Rubiaceae) is a neotropical genus with three centers of diversity in Mexico, Brazil and the Caribbean. Regarding Mitracarpus baturitensis, there is still no report in lieratura phytochemical and biological studies due to it being a newly discovered species. Therefore the aim of this study was to evaluate the antimicrobial activity of the ethanolic extract obtained composts of parts of flights Mitracarpus bauritensis against the bacteria Streptococcus mutans. Compounds ursolic acid (3β-hidroxi-urs-12-en-28-oico acid) and pomolic acid (28- O-β-D-glucopiranosil- Ãcido pomÃlico ester) were isolated from the ethanolic extract by chromatographic assays, CCD and identified through the RNM. After identification were performed testing the minimum inhibitory concentration (MIC), minimum bactericidal concentration (CBM), curve of the time of the death, action antibiofilm, assay attack of the biofilm. The increase inhibited bacterial growth at concentrations from 500 to 62,5 Âg. mL-1 and bactericide concentration of 500 Âg. mL-1. Furthermore, the AU had an effect on biofilm formation by reducing the biomass 97,37% same result is not obtained on the biofilm for 24 hours. Similar results were not obtained for the AP. Thus, AU can be analized as a candidate for…

Advisors/Committee Members: Maria Rose Jane Ribeiro Albuquerque, Edson Holanda Teixeira, Francisco Vassiliepe Sousa Arruda.

Subjects/Keywords: MICROBIOLOGIA; Mitracarpus baturitensis; Streptococcus mutans; Isolamento; Atividade antimicrobiana.; Mitracarpus baturitensis; Streptococcus mutans; Isolation; Antimicrobial activity.; Streptococcus mutans

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Bastos, R. M. (2013). AvaliaÃÃo da atividade antimicrobiana dos compostos isolados da planta Mitracarpus Baturitensis frente Ã Streptococcus Mutans. (Masters Thesis). Universidade Federal do Ceará. Retrieved from http://www.teses.ufc.br/tde_busca/arquivo.php?codArquivo=13019 ;

Chicago Manual of Style (16^th Edition):

Bastos, Rafaela Mesquita. “AvaliaÃÃo da atividade antimicrobiana dos compostos isolados da planta Mitracarpus Baturitensis frente Ã Streptococcus Mutans.” 2013. Masters Thesis, Universidade Federal do Ceará. Accessed January 24, 2021. http://www.teses.ufc.br/tde_busca/arquivo.php?codArquivo=13019 ;.

MLA Handbook (7^th Edition):

Bastos, Rafaela Mesquita. “AvaliaÃÃo da atividade antimicrobiana dos compostos isolados da planta Mitracarpus Baturitensis frente Ã Streptococcus Mutans.” 2013. Web. 24 Jan 2021.

Vancouver:

Bastos RM. AvaliaÃÃo da atividade antimicrobiana dos compostos isolados da planta Mitracarpus Baturitensis frente Ã Streptococcus Mutans. [Internet] [Masters thesis]. Universidade Federal do Ceará 2013. [cited 2021 Jan 24]. Available from: http://www.teses.ufc.br/tde_busca/arquivo.php?codArquivo=13019 ;.

Council of Science Editors:

Bastos RM. AvaliaÃÃo da atividade antimicrobiana dos compostos isolados da planta Mitracarpus Baturitensis frente Ã Streptococcus Mutans. [Masters Thesis]. Universidade Federal do Ceará 2013. Available from: http://www.teses.ufc.br/tde_busca/arquivo.php?codArquivo=13019 ;

26. Sakaue, Yuuki. Anti-biofilm and bactericidal effects of magnolia bark-derived magnolol and honokiol on Streptococcus mutans : Streptococcus mutansに対するMagnololおよびHonokiolの殺菌ならびに抗バイオフィルム効果.

Degree: 博士（歯学）, 2016, Niigata University / 新潟大学

URL: http://hdl.handle.net/10191/45110

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学位の種類: 博士（歯学）. 報告番号: 甲第4224号. 学位記番号: 新大院博（歯）甲第366号. 学位授与年月日: 平成28年9月20日

Microbiology and Immunology 2016; 60: 10–16

Dental caries affects people of all ages and is a worldwide… (more)

Subjects/Keywords: honokiol; magnolia bark; magnolol; Streptococcus mutans

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Sakaue, Y. (2016). Anti-biofilm and bactericidal effects of magnolia bark-derived magnolol and honokiol on Streptococcus mutans : Streptococcus mutansに対するMagnololおよびHonokiolの殺菌ならびに抗バイオフィルム効果. (Thesis). Niigata University / 新潟大学. Retrieved from http://hdl.handle.net/10191/45110

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Sakaue, Yuuki. “Anti-biofilm and bactericidal effects of magnolia bark-derived magnolol and honokiol on Streptococcus mutans : Streptococcus mutansに対するMagnololおよびHonokiolの殺菌ならびに抗バイオフィルム効果.” 2016. Thesis, Niigata University / 新潟大学. Accessed January 24, 2021. http://hdl.handle.net/10191/45110.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Sakaue, Yuuki. “Anti-biofilm and bactericidal effects of magnolia bark-derived magnolol and honokiol on Streptococcus mutans : Streptococcus mutansに対するMagnololおよびHonokiolの殺菌ならびに抗バイオフィルム効果.” 2016. Web. 24 Jan 2021.

Vancouver:

Sakaue Y. Anti-biofilm and bactericidal effects of magnolia bark-derived magnolol and honokiol on Streptococcus mutans : Streptococcus mutansに対するMagnololおよびHonokiolの殺菌ならびに抗バイオフィルム効果. [Internet] [Thesis]. Niigata University / 新潟大学; 2016. [cited 2021 Jan 24]. Available from: http://hdl.handle.net/10191/45110.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Sakaue Y. Anti-biofilm and bactericidal effects of magnolia bark-derived magnolol and honokiol on Streptococcus mutans : Streptococcus mutansに対するMagnololおよびHonokiolの殺菌ならびに抗バイオフィルム効果. [Thesis]. Niigata University / 新潟大学; 2016. Available from: http://hdl.handle.net/10191/45110

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Rochester

27. Buckley, Andrew Alexander. Role of β-Phosphoglucomutase in Carbon Metabolism and Stress Responsiveness of Streptococcus mutans.

Degree: PhD, 2014, University of Rochester

URL: http://hdl.handle.net/1802/28964

► Streptococcus mutans, the causative agent of dental caries, resides in the microbiological niche known as dental plaque, located on the enamel surfaces of teeth in… (more)

▼ Streptococcus mutans, the causative agent of dental caries, resides in the microbiological niche known as dental plaque, located on the enamel surfaces of teeth in the oral cavity. As the result of fermentation of dietary carbohydrates, S. mutans produces organic acids that lead to the acidification of the plaque microenvironment. When the pH falls below a pH of 5.5, demineralization of the tooth enamel occurs, and the caries process may ensue if plaque is not removed. In response to the surrounding low pH, S. mutans utilizes multiple acid-adaptive mechanisms. In particular, changes in the fatty acid composition of the membrane, maintenance of a cytosolic pH more basic than the environment, and alteration of the metabolic profile are hallmark acid-tolerance strategies. The ability of S. mutans to alter the pH of its environment, and survive, allows it compete with commensal organisms in the plaque biofilm that cannot tolerate the low pH. In a genomic screen for acid sensitive mutant strains, a hypothetical gene, SMU.1747c, was identified as a candidate. Subsequently, SMU.1747c was identified as a β-phosphoglucomutase (β– Pgm). The presence of β–Pgm correlated with the ability to convert β-glucose-1- phosphate to glucose-6-phosphate (G6P) in a reaction coupled to G6P-dehydrogenase. The absence of pgmB resulted in an acid sensitive phenotype and a reduced generation rate in acidified medium. In addition to the physiological characterization of pgmB, we elucidated the transcriptional control of the gene. Expression of pgmB was upregulated under neutral pH conditions and in the absence of preferred carbohydrates, notably glucose. Growth in the presence of maltose led to the largest increase in transcription observed in these studies. In S. mutans, maltose induces the storage of intracellular polysaccharides (IPS), for use when exogenous carbohydrates are limited. Under conditions of IPS depletion, we also observed elevated transcription of pgmB. Expression of pgmB during growth in maltose, and after IPS-depletion, represents conditions known to involve the global regulators of carbon metabolism, CodY and CcpA. The results of this study show that pgmB regulation is dependent on nutrient conditions, and that β–Pgm activity is connected to non-preferred carbohydrate sources and IPS metabolism.

Subjects/Keywords: Streptococcus Mutans; Sugar Metabolism; Acid Tolerance; Regulation

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Buckley, A. A. (2014). Role of β-Phosphoglucomutase in Carbon Metabolism and Stress Responsiveness of Streptococcus mutans. (Doctoral Dissertation). University of Rochester. Retrieved from http://hdl.handle.net/1802/28964

Chicago Manual of Style (16^th Edition):

Buckley, Andrew Alexander. “Role of β-Phosphoglucomutase in Carbon Metabolism and Stress Responsiveness of Streptococcus mutans.” 2014. Doctoral Dissertation, University of Rochester. Accessed January 24, 2021. http://hdl.handle.net/1802/28964.

MLA Handbook (7^th Edition):

Buckley, Andrew Alexander. “Role of β-Phosphoglucomutase in Carbon Metabolism and Stress Responsiveness of Streptococcus mutans.” 2014. Web. 24 Jan 2021.

Vancouver:

Buckley AA. Role of β-Phosphoglucomutase in Carbon Metabolism and Stress Responsiveness of Streptococcus mutans. [Internet] [Doctoral dissertation]. University of Rochester; 2014. [cited 2021 Jan 24]. Available from: http://hdl.handle.net/1802/28964.

Council of Science Editors:

Buckley AA. Role of β-Phosphoglucomutase in Carbon Metabolism and Stress Responsiveness of Streptococcus mutans. [Doctoral Dissertation]. University of Rochester; 2014. Available from: http://hdl.handle.net/1802/28964

University of Rochester

28. Ramos, Isamar Rivera. Characterization of the Spx Global Regulator in Streptococcus mutans.

Degree: PhD, 2015, University of Rochester

URL: http://hdl.handle.net/1802/29365

► Streptococcus mutans is considered to play a major role in the development of dental caries. The organism’s ability to form biofilms on teeth and to… (more)

▼ Streptococcus mutans is considered to play a major role in the development of dental caries. The organism’s ability to form biofilms on teeth and to adapt to the constantly changing environment of the oral cavity are intertwined with its pathogenic potential. Among the stresses encountered in the oral cavity, fluctuations in pH and oxygen tension have the greatest impact on biofilm ecology and caries outcome. In Gram-positive bacteria, the transcriptional regulator Spx was shown to positively regulate genes involved in oxidative stress, as well as other important virulence traits. In Bacillus subtilis, accumulation of Spx was linked to phenotypes observed in strains lacking the ClpXP proteolytic system. In S. mutans, two spx homologs, spxA and spxB, were also shown to reverse phenotypes observed in ΔclpP and ΔclpX strains. Despite its association with the ClpXP system, the effects of global Spx regulation in pathogenic bacteria have been poorly characterized. Here, we conducted a thorough characterization of the two spx genes of S. mutans. Oxidative and acid stress sensitivities were reported for the ΔspxA and ΔspxAΔspxB strains. Although ΔspxB did not show any inherent stress-sensitive phenotype, the phenotypes observed in ΔspxA were more pronounced in the ΔspxAΔspxB strain. Using two in vivo models, we demonstrated that Spx regulation is required for full virulence of S. mutans. Transcription of both genes was not altered during oxidative stress but spxB levels were significantly induced at low pH. In vitro assays showed that ClpXP specifically targets both SpxA and SpxB for degradation. Microarrays revealed the regulation of several oxidative stress genes by SpxA and, to a less extent, SpxB. SpxB was also shown to regulate genes involved in fatty acid synthesis, cell division and cell wall homeostasis. The direct regulation of oxidative stress genes by SpxA was confirmed through in vitro transcription assays. The ability of SpxA to exert transcriptional control was dependent on the oxidized state of the protein, as well as on the presence of a conserved glycine residue. These experiments showed that SpxA and SpxB share functions or work independently to regulate virulence attributes and contribute to the cells oxidative stress survival.

Subjects/Keywords: Spx; Streptococcus Mutans; Transcription Regulation; Oxidative Stress

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Ramos, I. R. (2015). Characterization of the Spx Global Regulator in Streptococcus mutans. (Doctoral Dissertation). University of Rochester. Retrieved from http://hdl.handle.net/1802/29365

Chicago Manual of Style (16^th Edition):

Ramos, Isamar Rivera. “Characterization of the Spx Global Regulator in Streptococcus mutans.” 2015. Doctoral Dissertation, University of Rochester. Accessed January 24, 2021. http://hdl.handle.net/1802/29365.

MLA Handbook (7^th Edition):

Ramos, Isamar Rivera. “Characterization of the Spx Global Regulator in Streptococcus mutans.” 2015. Web. 24 Jan 2021.

Vancouver:

Ramos IR. Characterization of the Spx Global Regulator in Streptococcus mutans. [Internet] [Doctoral dissertation]. University of Rochester; 2015. [cited 2021 Jan 24]. Available from: http://hdl.handle.net/1802/29365.

Council of Science Editors:

Ramos IR. Characterization of the Spx Global Regulator in Streptococcus mutans. [Doctoral Dissertation]. University of Rochester; 2015. Available from: http://hdl.handle.net/1802/29365

University of Rochester

29. Cross, Benjamin W. The Role of PlsX in Fatty Acid Synthesis and Acid Adaptation in Streptococcus mutans.

Degree: PhD, 2016, University of Rochester

URL: http://hdl.handle.net/1802/30973

► Streptococcus mutans is one of the primary causative agents of dental caries in humans. S. mutans ferments dietary sugars in the mouth to produce organic… (more)

▼ Streptococcus mutans is one of the primary causative agents of dental caries in humans. S. mutans ferments dietary sugars in the mouth to produce organic acids. These acids lower local pH values resulting in demineralization of the tooth enamel, leading to caries. To survive acidic environments, S. mutans employs several adaptive mechanisms, including a shift from saturated to unsaturated fatty acids in membrane phospholipids. Evidence suggests that this shift requires de novo fatty acid and phospholipid synthesis; therefore, understanding these synthesis pathways is crucial for understanding how S. mutans adapts to low pH and causes caries. PlsX is an acyl- ACP:phosphate transacylase that links the fatty acid synthesis pathway to the phospholipid synthesis pathway, and is central to the movement of unsaturated fatty acids into the membrane. It has recently been discovered that plsX is not essential in S. mutans. This study explores how the loss of plsX affects the ability of S. mutans to alter its membrane fatty acid profile and survive at low pH. The plsX deletion mutant (ΔplsX) is not a fatty acid or phospholipid auxotroph, indicating that some alternative pathway is capable of carrying out the first step of phospholipid synthesis. Gas chromatography of fatty acid methyl esters (GC-FAME) indicates that deletion of plsX impacts the regulation of fatty acid synthesis, altering the length and saturation of fatty acids. Surprisingly, ΔplsX survives significantly longer than the parent strain, UA159, when subjected to an acid challenge of pH 2.5. This enhanced survival may be due to the increased F-ATPase activity observed at low pH. This enhanced F-ATPase activity may be due to the altered fatty acid profile, or may be part of a response to membrane stress. Supplementing ΔplsX with exogenous unsaturated fatty acids does not restore any wild-type phenotypes; however, incorporation of exogenous fatty acids is 2-fold greater in ΔplsX, compared to UA159. Exogenous oleic acid was observed to decrease survival in acid challenge for both ΔplsX and UA159. These results clearly indicate that the loss of plsX affects both the fatty acid synthesis pathway and the acid-adaptive response of S. mutans.

Subjects/Keywords: Acid; Caries; Fatty acid; Mutans; PlsX; Streptococcus

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Cross, B. W. (2016). The Role of PlsX in Fatty Acid Synthesis and Acid Adaptation in Streptococcus mutans. (Doctoral Dissertation). University of Rochester. Retrieved from http://hdl.handle.net/1802/30973

Chicago Manual of Style (16^th Edition):

Cross, Benjamin W. “The Role of PlsX in Fatty Acid Synthesis and Acid Adaptation in Streptococcus mutans.” 2016. Doctoral Dissertation, University of Rochester. Accessed January 24, 2021. http://hdl.handle.net/1802/30973.

MLA Handbook (7^th Edition):

Cross, Benjamin W. “The Role of PlsX in Fatty Acid Synthesis and Acid Adaptation in Streptococcus mutans.” 2016. Web. 24 Jan 2021.

Vancouver:

Cross BW. The Role of PlsX in Fatty Acid Synthesis and Acid Adaptation in Streptococcus mutans. [Internet] [Doctoral dissertation]. University of Rochester; 2016. [cited 2021 Jan 24]. Available from: http://hdl.handle.net/1802/30973.

Council of Science Editors:

Cross BW. The Role of PlsX in Fatty Acid Synthesis and Acid Adaptation in Streptococcus mutans. [Doctoral Dissertation]. University of Rochester; 2016. Available from: http://hdl.handle.net/1802/30973

30. Bautista Manrique, Roselena. Efecto antibacteriano de la miel de abeja en diferentes concentraciones sobre el Estreptococo Mutans.

Degree: CIENCIAS DE LA SALUD, 2011, Universidad de San Martín de Porres. Programa Cybertesis PERÚ

URL: http://cybertesis.usmp.edu.pe/usmp/2011/bautista_ro/html/index-frames.html

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Determinar el efecto antibacteriano de la miel de abeja en diferentes concentraciones sobre el Estreptococos mutans. Estudio experimental en 50 placas petri con Estreptococo mutans… (more)

Subjects/Keywords: Antibacterianos; Estreptococo Mutans; Miel de abejas

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Bautista Manrique, R. (2011). Efecto antibacteriano de la miel de abeja en diferentes concentraciones sobre el Estreptococo Mutans. (Thesis). Universidad de San Martín de Porres. Programa Cybertesis PERÚ. Retrieved from http://cybertesis.usmp.edu.pe/usmp/2011/bautista_ro/html/index-frames.html

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Bautista Manrique, Roselena. “Efecto antibacteriano de la miel de abeja en diferentes concentraciones sobre el Estreptococo Mutans.” 2011. Thesis, Universidad de San Martín de Porres. Programa Cybertesis PERÚ. Accessed January 24, 2021. http://cybertesis.usmp.edu.pe/usmp/2011/bautista_ro/html/index-frames.html.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Bautista Manrique, Roselena. “Efecto antibacteriano de la miel de abeja en diferentes concentraciones sobre el Estreptococo Mutans.” 2011. Web. 24 Jan 2021.

Vancouver:

Bautista Manrique R. Efecto antibacteriano de la miel de abeja en diferentes concentraciones sobre el Estreptococo Mutans. [Internet] [Thesis]. Universidad de San Martín de Porres. Programa Cybertesis PERÚ; 2011. [cited 2021 Jan 24]. Available from: http://cybertesis.usmp.edu.pe/usmp/2011/bautista_ro/html/index-frames.html.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Bautista Manrique R. Efecto antibacteriano de la miel de abeja en diferentes concentraciones sobre el Estreptococo Mutans. [Thesis]. Universidad de San Martín de Porres. Programa Cybertesis PERÚ; 2011. Available from: http://cybertesis.usmp.edu.pe/usmp/2011/bautista_ro/html/index-frames.html

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

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