You searched for subject:(Molecular imaging).
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Ryerson University
1.
Hussein, Farah.
On the molecular release induced by ultrasound and microbubbles in cells.
Degree: 2014, Ryerson University
URL: https://digital.library.ryerson.ca/islandora/object/RULA%3A3414 
► Ultrasound and microbubble (USMB) enhances intracellular uptake through membrane disruption and endocytosis. This study investigates USMB effects on the molecular release incells through membrane-disruption and…
(more)
▼ Ultrasound and microbubble (USMB) enhances intracellular uptake through membrane disruption and endocytosis. This study investigates USMB effects on the
molecular release incells through membrane-disruption and exocytosis. Retinal pigmented epithelial (RPE) cells were loaded with Alexa 647-transferrin (Tfn) to mark recycling endosomes, LAMP-1
antibody was used to mark lysosomes, GFP-transfected RPE cells were used to mark cytoplasm, and 7-AAD was used to assess cell viability. USMB exposure was done at 570kPa peak negative pressure for 1min. The mean fluorescent intensities (MFI) of markers were measured using flow cytometry. USMB induced the release of 19% and 67% of GFP from the cytoplasm in viable and non-viable cells respectively. LAMP-1 antibody MFI increased by 50% and 15-folds in viable and non-viable cells indicating USMB induced release from lysosomes. Furthermore, Tfn release from recycling endosomes increased by 22% only in viable cells. In conclusion, USMB enhances the
molecular release from cytoplasm, lysosomes, and recycling endosomes
Advisors/Committee Members: Ryerson University (Degree grantor).
Subjects/Keywords: Cytology; Ultrasonic imaging; Molecular neurobiology
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APA (6th Edition):
Hussein, F. (2014). On the molecular release induced by ultrasound and microbubbles in cells. (Thesis). Ryerson University. Retrieved from https://digital.library.ryerson.ca/islandora/object/RULA%3A3414
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Hussein, Farah. “On the molecular release induced by ultrasound and microbubbles in cells.” 2014. Thesis, Ryerson University. Accessed September 17, 2019.
https://digital.library.ryerson.ca/islandora/object/RULA%3A3414.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Hussein, Farah. “On the molecular release induced by ultrasound and microbubbles in cells.” 2014. Web. 17 Sep 2019.
Vancouver:
Hussein F. On the molecular release induced by ultrasound and microbubbles in cells. [Internet] [Thesis]. Ryerson University; 2014. [cited 2019 Sep 17].
Available from: https://digital.library.ryerson.ca/islandora/object/RULA%3A3414.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Hussein F. On the molecular release induced by ultrasound and microbubbles in cells. [Thesis]. Ryerson University; 2014. Available from: https://digital.library.ryerson.ca/islandora/object/RULA%3A3414
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
McMaster University
2.
Vito, Alyssa.
Preparation and Evaluation of Molecular Imaging Probes Targeting the Urokinase Plasminogen Activator System.
Degree: MSc, 2015, McMaster University
URL: http://hdl.handle.net/11375/18443
► The aim of this thesis was to develop a molecular imaging probe for the urokinase plasminogen activator (uPA) system, which has been shown to play…
(more)
▼ The aim of this thesis was to develop a molecular imaging probe for the urokinase plasminogen activator (uPA) system, which has been shown to play a critical role in cancer metastasis, tumour aggressiveness and likelihood of progression. Two classes of small molecule inhibitors carrying isotopes of iodine were synthesized and evaluated using in vitro assays and in vivo studies. Lead compounds showed high affinity for the target with Ki values in the low nanomolar range (1b = 1.4 nM, 1e = 6.1 nM, 1g = 2.6 nM and 2a = 2.1 nM). Biodistribution studies of the reversible compounds (1b, 1e, 1g) showed rapid clearance, accumulation in the gall bladder and intestines and little to no tumour uptake (<1 %ID/g). The irreversible inhibitor (2a) showed specificity for the target through SDS-PAGE and biodistribution studies. Analysis of the biodistribution pattern showed retention in the tumour over time reaching a maximum at 24 h post-injection of 1.95 %ID/g with tumour-to-blood ratio being 0.65 at 24 h, 1.13 at 48 h and 1.09 at 96 h post-injection.
A parallel strategy reported involved targeting the uPA receptor (uPAR) through the use of antibodies and bioorthogonal chemisty based on radiolabeled tetrazines and transcyclooctene (TCO) functionalized biomolecules. A new tetrazine synthon was developed that can be readily labeled with both 99mTc and 18F where the products were produced in 75 and 31 % radiochemical yields. Stability studies showed the compounds are suitable for use in vivo. Biodistribution studies were carried out in CD1 mice and results showed that both probes had sufficient distribution patterns to warrant use in pre-targeting strategies. Their reactivity with TCO, including functionalized derivatives such as TCO-anti-uPAR, was also demonstrated creating the means to develop PET and SPECT probes for imaging the urokinase system using a single prosthetic group.
Thesis
Master of Science (MSc)
Advisors/Committee Members: Valliant, John, Chemical Biology.
Subjects/Keywords: upa; cancer; molecular imaging; radiochemistry
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APA (6th Edition):
Vito, A. (2015). Preparation and Evaluation of Molecular Imaging Probes Targeting the Urokinase Plasminogen Activator System. (Masters Thesis). McMaster University. Retrieved from http://hdl.handle.net/11375/18443
Chicago Manual of Style (16th Edition):
Vito, Alyssa. “Preparation and Evaluation of Molecular Imaging Probes Targeting the Urokinase Plasminogen Activator System.” 2015. Masters Thesis, McMaster University. Accessed September 17, 2019.
http://hdl.handle.net/11375/18443.
MLA Handbook (7th Edition):
Vito, Alyssa. “Preparation and Evaluation of Molecular Imaging Probes Targeting the Urokinase Plasminogen Activator System.” 2015. Web. 17 Sep 2019.
Vancouver:
Vito A. Preparation and Evaluation of Molecular Imaging Probes Targeting the Urokinase Plasminogen Activator System. [Internet] [Masters thesis]. McMaster University; 2015. [cited 2019 Sep 17].
Available from: http://hdl.handle.net/11375/18443.
Council of Science Editors:
Vito A. Preparation and Evaluation of Molecular Imaging Probes Targeting the Urokinase Plasminogen Activator System. [Masters Thesis]. McMaster University; 2015. Available from: http://hdl.handle.net/11375/18443
McMaster University
3.
LLANO PIEDRA, LISSET BARBARA.
TOWARD MOLECULAR IMAGING PROBES TO DETECT CRYPTIC BACTERIAL INFECTIONS.
Degree: MSc, 2016, McMaster University
URL: http://hdl.handle.net/11375/18688
► Infectious diseases represent one of the leading causes of death globally. Prompt diagnosis is essential for the onset of clinical treatment but certain cases of…
(more)
▼ Infectious diseases represent one of the leading causes of death globally. Prompt diagnosis is essential for the onset of clinical treatment but certain cases of underlying bacterial infection deep in the body can remain undiagnosed for weeks. Hidden bacterial infection is the leading cause of fever of unknown origin (FUO), which is observed in 2 % of all hospital admissions around the world. Molecular imaging of bacterial infections is the ideal non-invasive diagnostic tool, but all available probes also detect inflammation. Two targets were selected for development of bacteria-specific molecular imaging probes, namely iron-uptake pathways and peptidoglycans involved in the synthesis of the cell wall. Both, Gram-positive and Gram-negative bacteria use iron-binding molecules called siderophores to scavenge iron from their surroundings. The structural similarities between Fe3+ and Ga3+ allow siderophores to be radiolabelled with 67/68Ga and visualized by nuclear medicine techniques. The clinically proven siderophore Deferoxamine (Dfo) has a plasma half-life of only 5.5 min that does not favor its direct use as a probe. Dfo derivatives with improved pharmacokinetics properties were designed and tested on Staphylococcus aureus cultures. The ciprofloxacin and the ethyloxycarbonyl derivatives of DFO at the primary amino position were among the most successful conjugates targeting the siderophore active-transport mechanism and reaching high relative uptake rates. Furthermore, the peptidoglycan pathway of Gram-positive bacteria was in vitro targeted with vancomycin conjugated to 67Ga-Dfo which showed even higher labelling capacity than 67Ga-Dfo within a few minutes of exposure. In vitro siderophore studies remain challenging due to the lack of methods for the preparation of rigorously iron-depleted media. We developed an iron chelating method with the goal of creating iron-free growth media.
Thesis
Master of Science (MSc)
Advisors/Committee Members: BERTI, PAUL, Chemical Biology.
Subjects/Keywords: molecular imaging of bacterial infection
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APA (6th Edition):
LLANO PIEDRA, L. B. (2016). TOWARD MOLECULAR IMAGING PROBES TO DETECT CRYPTIC BACTERIAL INFECTIONS. (Masters Thesis). McMaster University. Retrieved from http://hdl.handle.net/11375/18688
Chicago Manual of Style (16th Edition):
LLANO PIEDRA, LISSET BARBARA. “TOWARD MOLECULAR IMAGING PROBES TO DETECT CRYPTIC BACTERIAL INFECTIONS.” 2016. Masters Thesis, McMaster University. Accessed September 17, 2019.
http://hdl.handle.net/11375/18688.
MLA Handbook (7th Edition):
LLANO PIEDRA, LISSET BARBARA. “TOWARD MOLECULAR IMAGING PROBES TO DETECT CRYPTIC BACTERIAL INFECTIONS.” 2016. Web. 17 Sep 2019.
Vancouver:
LLANO PIEDRA LB. TOWARD MOLECULAR IMAGING PROBES TO DETECT CRYPTIC BACTERIAL INFECTIONS. [Internet] [Masters thesis]. McMaster University; 2016. [cited 2019 Sep 17].
Available from: http://hdl.handle.net/11375/18688.
Council of Science Editors:
LLANO PIEDRA LB. TOWARD MOLECULAR IMAGING PROBES TO DETECT CRYPTIC BACTERIAL INFECTIONS. [Masters Thesis]. McMaster University; 2016. Available from: http://hdl.handle.net/11375/18688
Université Catholique de Louvain
4.
Neveu, Marie-Aline.
Multi-modality imaging to assess metabolic shift in tumors.
Degree: 2016, Université Catholique de Louvain
URL: http://hdl.handle.net/2078.1/179477
► The objective of the thesis was to explore the value of different noninvasive imaging technologies to assess tumor metabolic profiles in vivo and their pharmacological…
(more)
▼ The objective of the thesis was to explore the value of different noninvasive imaging technologies to assess tumor metabolic profiles in vivo and their pharmacological modulations. Adaptive behaviors supporting a deregulated metabolism in cancer represent major challenges for therapies. Therefore, a better understanding of these processes associated with the characterization of metabolic patterns in tumors would lead to the development of more efficient anticancer strategies. In parallel to in vitro characterizations, advanced imaging techniques including EPR oximetry, 13C-hyperpolarized NMR, 17O MRS and 18F-FDG PET, were used to identify the metabolic signature in well-established tumor models in vivo. Our study reveals major discordances between in vitro and in vivo metabolic profiles, highlighting the limitation of in vitro studies to truly reflect the complex tumor behavior. Using imaging modalities, we confirmed the key role of local microenvironment to shape tumor features.
(BIFA - Sciences biomédicales et pharmaceutiques) – UCL, 2016
Advisors/Committee Members: UCL - SSS/LDRI - Louvain Drug Research Institute, UCL - SSS/IREC - Institut de recherche expérimentale et clinique, UCL - Faculté de pharmacie et des sciences biomédicales, Feron, Olivier, Delzenne, Nathalie, Jordan, Bénédicte, Bindels, Laure, Grégoire, Vincent, Gallez, Bernard, Ardenkjaer-Larsen, Jan Henrik, Scheenen, Tom.
Subjects/Keywords: Molecular imaging; Tumor metabolism
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APA (6th Edition):
Neveu, M. (2016). Multi-modality imaging to assess metabolic shift in tumors. (Thesis). Université Catholique de Louvain. Retrieved from http://hdl.handle.net/2078.1/179477
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Neveu, Marie-Aline. “Multi-modality imaging to assess metabolic shift in tumors.” 2016. Thesis, Université Catholique de Louvain. Accessed September 17, 2019.
http://hdl.handle.net/2078.1/179477.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Neveu, Marie-Aline. “Multi-modality imaging to assess metabolic shift in tumors.” 2016. Web. 17 Sep 2019.
Vancouver:
Neveu M. Multi-modality imaging to assess metabolic shift in tumors. [Internet] [Thesis]. Université Catholique de Louvain; 2016. [cited 2019 Sep 17].
Available from: http://hdl.handle.net/2078.1/179477.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Neveu M. Multi-modality imaging to assess metabolic shift in tumors. [Thesis]. Université Catholique de Louvain; 2016. Available from: http://hdl.handle.net/2078.1/179477
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Johannes Gutenberg Universität Mainz
5.
Schieferstein, Hanno.
Folic acid derivatives for PET imaging and therapy addressing folate receptor positive tumors.
Degree: 2013, Johannes Gutenberg Universität Mainz
URL: http://ubm.opus.hbz-nrw.de/volltexte/2013/3603/
► Folic acid, also known as vitamin B9, is the oxidized form of 5,6,7,8-tetrahydrofolate, which serves as methyl- or methylene donor (C1-building blocks) during DNA synthesis.…
(more)
▼ Folic acid, also known as vitamin B9, is the oxidized form of 5,6,7,8-tetrahydrofolate, which serves as methyl- or methylene donor (C1-building blocks) during DNA synthesis. Under physiological conditions the required amount of 5,6,7,8-tetrahydrofolate for survival of the cell is accomplished through the reduced folate carrier (RFC). In contrast, the supply of 5,6,7,8-tetrahydrofolate is insufficient under pathophysiological conditions of tumors due to an increased proliferation rate. Consequently, many tumor cells exhibit an (over)expression of the folate receptor. This phenomenon has been applied to diagnostics (PET, SPECT, MR) to image FR-positive tumors and on the other hand to treat malignancies related to a FR (over)expression. Based on this concept, a new 18F-labeled folate for PET imaging has been developed and was evaluated in vivo using tumor-bearing mice. The incorporation of oligoethylene spacers into the molecular structure led to a significant enhancement of the pharmacokinetics in comparison to previously developed 18F-folates. The liver uptake could be reduced by one sixth by remaining a tumor uptake of 3%ID/g leading to better contrast ratios. Encouraged by these results, a clickable 18F-labeled serine-based prosthetic group has been synthesized, again with the idea to improve the metabolic and pharmacokinetic profile of hydrophilic radiotracers. Therefore, an alkyne-carrying azido-functionalized serine derivative for coupling to biomolecules was synthesized and a chlorine leaving group for 18F-labeling, which could be accomplished using a microwave-assisted synthesis, a [K⊂2.2.2]+/carbonate system in DMSO. Radiochemical yields of 77±6% could be achieved.rnThe promising results obtained from the FR-targeting concept in the diagnostic field have been transferred to the boron neutron capture therapy. Therefore, a folate derivative was coupled to different boron clusters and cell uptake studies were conducted. The synthesis of the folate-boron clusters was straightforward. At first, a linker molecule based on maleic acid was synthesized, which was coupled to the boron cluster via Michael Addition of a thiol and alkene and subsequently coupled to the targeting moiety using CuAAC. The new conjugates of folate and boron clusters led to a significant increase of boron concentration in the cell of about 5-times compared to currently used and approved boron pharmaceuticals. rnMoreover, azido-folate derivatives were coupled to macromolecular carrier systems (pHPMA), which showed an enhanced and specific accumulation at target sites (up to 2.5-times) during in vivo experiments. A specific blockade could be observed up to 30% indicating an efficient targeting effect. A new kind of nanoparticles consisting of a PDLLA core and p((HPMA)-b-LMA)) as surfactants were developed and successfully radiolabeled via 18F-click chemistry in good RCYs of 8±3%rnThe nanoparticles were obtained via the miniemulsion technique in combination with solvent evaporation. The 18F-labeled nanoparticles were applied to in vivo testing…
Subjects/Keywords: Molecular Imaging; Chemistry and allied sciences
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APA ·
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Manager
APA (6th Edition):
Schieferstein, H. (2013). Folic acid derivatives for PET imaging and therapy addressing folate receptor positive tumors. (Doctoral Dissertation). Johannes Gutenberg Universität Mainz. Retrieved from http://ubm.opus.hbz-nrw.de/volltexte/2013/3603/
Chicago Manual of Style (16th Edition):
Schieferstein, Hanno. “Folic acid derivatives for PET imaging and therapy addressing folate receptor positive tumors.” 2013. Doctoral Dissertation, Johannes Gutenberg Universität Mainz. Accessed September 17, 2019.
http://ubm.opus.hbz-nrw.de/volltexte/2013/3603/.
MLA Handbook (7th Edition):
Schieferstein, Hanno. “Folic acid derivatives for PET imaging and therapy addressing folate receptor positive tumors.” 2013. Web. 17 Sep 2019.
Vancouver:
Schieferstein H. Folic acid derivatives for PET imaging and therapy addressing folate receptor positive tumors. [Internet] [Doctoral dissertation]. Johannes Gutenberg Universität Mainz; 2013. [cited 2019 Sep 17].
Available from: http://ubm.opus.hbz-nrw.de/volltexte/2013/3603/.
Council of Science Editors:
Schieferstein H. Folic acid derivatives for PET imaging and therapy addressing folate receptor positive tumors. [Doctoral Dissertation]. Johannes Gutenberg Universität Mainz; 2013. Available from: http://ubm.opus.hbz-nrw.de/volltexte/2013/3603/
Washington University in St. Louis
6.
Zhang, Oumeng.
Measuring Molecular Orientation and Rotational Mobility Using a Tri-spot Point Spread Function.
Degree: MS, Electrical & Systems Engineering, 2017, Washington University in St. Louis
URL: https://openscholarship.wustl.edu/eng_etds/226
► Single molecules have become a powerful tool for biophysicists since they were first optically detected 28 years ago. Understanding molecular orientation can not only…
(more)
▼ Single molecules have become a powerful tool for biophysicists since they were first optically detected 28 years ago. Understanding
molecular orientation can not only improve the accuracy of single-molecule localization, but it can also provide insight into biochemical behaviors at the nanoscale. In this thesis, I present a method to measure the
molecular orientation and rotational mobility of single-molecule emitters by designing and implementing a tri-spot point spread function. The point spread function is designed so that it is capable of measuring all degrees of freedom related to
molecular orientation and rotational mobility. Its design is optimized by maximizing the theoretical limit of measurement precision. Two methods, basis inversion and maximum likelihood, are used to estimate the
molecular orientation and rotational mobility. The basis inversion method was demonstrated experimentally with fluorescent beads. The maximum likelihood estimator approaches the theoretical limit of accuracy and precision in simulations, and is used to measure experimentally the orientation of single fluorescent molecules embedded in a polymer matrix.
Advisors/Committee Members: R. Martin Arthur, Matthew D. Lew
Joseph A. O’Sullivan
Lan Yang.
Subjects/Keywords: single molecule imaging; molecular orientation; Engineering; Optics
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APA ·
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APA (6th Edition):
Zhang, O. (2017). Measuring Molecular Orientation and Rotational Mobility Using a Tri-spot Point Spread Function. (Thesis). Washington University in St. Louis. Retrieved from https://openscholarship.wustl.edu/eng_etds/226
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Zhang, Oumeng. “Measuring Molecular Orientation and Rotational Mobility Using a Tri-spot Point Spread Function.” 2017. Thesis, Washington University in St. Louis. Accessed September 17, 2019.
https://openscholarship.wustl.edu/eng_etds/226.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Zhang, Oumeng. “Measuring Molecular Orientation and Rotational Mobility Using a Tri-spot Point Spread Function.” 2017. Web. 17 Sep 2019.
Vancouver:
Zhang O. Measuring Molecular Orientation and Rotational Mobility Using a Tri-spot Point Spread Function. [Internet] [Thesis]. Washington University in St. Louis; 2017. [cited 2019 Sep 17].
Available from: https://openscholarship.wustl.edu/eng_etds/226.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Zhang O. Measuring Molecular Orientation and Rotational Mobility Using a Tri-spot Point Spread Function. [Thesis]. Washington University in St. Louis; 2017. Available from: https://openscholarship.wustl.edu/eng_etds/226
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
University of Edinburgh
7.
Joshi, Nikhil Vilas.
Novel molecular imaging of cardiovascular disease in man.
Degree: PhD, 2016, University of Edinburgh
URL: http://hdl.handle.net/1842/25394
► Cardiovascular disease remains the commonest cause of death worldwide. The majority of deaths are caused by atherosclerotic plaque rupture with resultant myocardial infarction or stroke,…
(more)
▼ Cardiovascular disease remains the commonest cause of death worldwide. The majority of deaths are caused by atherosclerotic plaque rupture with resultant myocardial infarction or stroke, or rupture of abdominal aortic aneurysms. Conventional imaging modalities have consistently failed to identify atherosclerotic plaques or aneurysms with high-risk pathological features that are at highest risk of rupture or progression. The development of modern molecular imaging techniques targeted at these features could lead to the identification of such high-risk plaques and aneurysms in vivo and guide the development of novel treatment strategies. The aim of this thesis was to evaluate whether novel molecular modalities have a role in providing new insights into biological disease processes, and identify high-risk plaques and aneurysms. Using positron emission tomography-computed tomography (PET-CT), 18F-fluorodeoxyglucose and 18F-fluoride were utilised as markers of metabolic inflammation and active calcification. Cellular inflammation was assessed using ultrasmall superparamagnetic particles of iron oxide (USPIO) enhanced magnetic resonance imaging (MRI). In a prospective trial, 80 patients with myocardial infarction (n=40) and stable angina (n=40) underwent 18F-fluoride and 18F-fluorodeoxyglucose PET-CT, and invasive coronary angiography (Chapter 3). Intense 18F-fluoride uptake localised to recently ruptured plaque in patients with acute myocardial infarction. In patients with stable coronary artery disease, 18F-fluoride uptake identified coronary plaques with high-risk features on intravascular ultrasound. 18F-fluoride PET-CT is the first noninvasive imaging method to identify and localise ruptured and high-risk coronary plaques. Aortic vascular uptake of 18F- fluorodeoxyglucose was studied in patients with myocardial infarction and stable angina (Chapter 4). In a separate outcome of 1,003 patients enrolled in the Global Registry of Acute Coronary Events, we further evaluated whether infarct size predicted recurrent coronary events. Patients with myocardial infarction had higher remote atherosclerotic tracer uptake that correlated with the degree of myocardial necrosis, and exceeded that observed in patients with stable coronary disease. The outcome cohort demonstrated that patients with higher degree of myocardial necrosis had the highest risk of early recurrent myocardial infarction. This supports the hypothesis that acute myocardial infarction exacerbates systemic atherosclerotic inflammation and remote plaque destabilization: myocardial infarction begets myocardial infarction. In a prospective imaging cohort, the role inflammation and calcification was assessed in 63 patients with abdominal aortic aneurysms and 19 age and sex matched patients with atherosclerosis (Chapter 5). Compared to non-aneurysmal segments, enhanced inflammation and calcification was observed within the wall of aortic aneurysmal segments. In comparison to matched controls with atherosclerosis, the entire aorta in those with aortic aneurysm appears…
Subjects/Keywords: 616.1; atherosclerosis; aneurysm; molecular imaging; PET-CT
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APA ·
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Manager
APA (6th Edition):
Joshi, N. V. (2016). Novel molecular imaging of cardiovascular disease in man. (Doctoral Dissertation). University of Edinburgh. Retrieved from http://hdl.handle.net/1842/25394
Chicago Manual of Style (16th Edition):
Joshi, Nikhil Vilas. “Novel molecular imaging of cardiovascular disease in man.” 2016. Doctoral Dissertation, University of Edinburgh. Accessed September 17, 2019.
http://hdl.handle.net/1842/25394.
MLA Handbook (7th Edition):
Joshi, Nikhil Vilas. “Novel molecular imaging of cardiovascular disease in man.” 2016. Web. 17 Sep 2019.
Vancouver:
Joshi NV. Novel molecular imaging of cardiovascular disease in man. [Internet] [Doctoral dissertation]. University of Edinburgh; 2016. [cited 2019 Sep 17].
Available from: http://hdl.handle.net/1842/25394.
Council of Science Editors:
Joshi NV. Novel molecular imaging of cardiovascular disease in man. [Doctoral Dissertation]. University of Edinburgh; 2016. Available from: http://hdl.handle.net/1842/25394
University of Ottawa
8.
Tayyabi, Ehsen.
Gone Fishing: Synthesis and Design of a Superparamagnetic Nanobait for Trapping Reactive Metabolites In Vivo.
Degree: 2018, University of Ottawa
URL: http://hdl.handle.net/10393/37338
► Adverse drug reactions are common causes of medical injuries. Drug-induced hepatotoxicity remains one of the leading causes of emergency room visits, FDA non-approval, and drug…
(more)
▼ Adverse drug reactions are common causes of medical injuries. Drug-induced hepatotoxicity remains one of the leading causes of emergency room visits, FDA non-approval, and drug withdrawal from the market. We have investigated the ability of endogenous nucleophilic amino acid residues (K, H, and C) to selectively bind to reactive electrophilic drug metabolites, focusing on acetyl-para-aminophenol (APAP, i.e. Tylenol®), for which hepatotoxicity has recently re- emerged as a major health concern for Canadians. Three peptide sequences were synthesized bearing terminal nucleophilic residues, brominated phenylalanine residues, and c-terminal amides. These peptides were coupled to carboxy methyl dextran coated iron oxide nanoparticles (CMX- IONPs) with a hepatocyte targeting group. IONPs are known for their ability to act as T2-weighted MRI contrast agents, giving us the ability to track them in vivo. This study begins to establish a nanotechnology-based method for the in vivo trapping of NAPQI, the reactive metabolite of APAP, using a cysteine bearing IONP.
Subjects/Keywords: Molecular Imaging;
Chemical Biology;
MRI;
Drug Metabolism
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APA ·
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APA (6th Edition):
Tayyabi, E. (2018). Gone Fishing: Synthesis and Design of a Superparamagnetic Nanobait for Trapping Reactive Metabolites In Vivo.
(Thesis). University of Ottawa. Retrieved from http://hdl.handle.net/10393/37338
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Tayyabi, Ehsen. “Gone Fishing: Synthesis and Design of a Superparamagnetic Nanobait for Trapping Reactive Metabolites In Vivo.
” 2018. Thesis, University of Ottawa. Accessed September 17, 2019.
http://hdl.handle.net/10393/37338.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Tayyabi, Ehsen. “Gone Fishing: Synthesis and Design of a Superparamagnetic Nanobait for Trapping Reactive Metabolites In Vivo.
” 2018. Web. 17 Sep 2019.
Vancouver:
Tayyabi E. Gone Fishing: Synthesis and Design of a Superparamagnetic Nanobait for Trapping Reactive Metabolites In Vivo.
[Internet] [Thesis]. University of Ottawa; 2018. [cited 2019 Sep 17].
Available from: http://hdl.handle.net/10393/37338.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Tayyabi E. Gone Fishing: Synthesis and Design of a Superparamagnetic Nanobait for Trapping Reactive Metabolites In Vivo.
[Thesis]. University of Ottawa; 2018. Available from: http://hdl.handle.net/10393/37338
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Uppsala University
9.
Lövgren, Jessica.
Site-specific labelling of anti-HER2 ADAPT proteins for molecular imaging applications.
Degree: Biology Education Centre, 2016, Uppsala University
URL: http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-302905
► Cancer is one of the leading causes of death worldwide. It is of great importance to use reliable diagnostic methods before determining treatment methods.…
(more)
▼ Cancer is one of the leading causes of death worldwide. It is of great importance to use reliable diagnostic methods before determining treatment methods. Molecular imaging is a non-invasive approach of visualising tumours in vivo. This method requires imaging agents that are detectable and binds specifically to the tumour. The aim of this degree project was to produce and characterise a protein-based imaging agent, called ADAPT, that has been engineered to bind the breast cancer associated receptor HER2. This protein has previously been labelled with radionuclides in the N-terminal and tested in vivo with promising results. However, due to the fact that the position of the radiolabel can affect the biodistribution of the imaging agent, a comparison to C-terminal radiolabelled variants is of high interest. Radiolabelling can be performed by first attaching a chelator to the protein and later bind the radionuclide through the chelator. The chelator used in this project binds to a thiol group and therefore a unique cysteine was added to the C-termini. The N-termini were altered to both generate variants with and without purification tag. Proteins with altered N-termini and C-termini were produced and later characterised by circular dichroism and surface plasmon resonance. None of the non-tagged variants could be produced to a sufficient amount and was discarded for further studies. However, all variants with a purification tag could be produced and the shortest variant was chosen to be tested in vivo.
Subjects/Keywords: cancer diagnostics; molecular imaging; ADAPT; HER2
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APA (6th Edition):
Lövgren, J. (2016). Site-specific labelling of anti-HER2 ADAPT proteins for molecular imaging applications. (Thesis). Uppsala University. Retrieved from http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-302905
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Lövgren, Jessica. “Site-specific labelling of anti-HER2 ADAPT proteins for molecular imaging applications.” 2016. Thesis, Uppsala University. Accessed September 17, 2019.
http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-302905.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Lövgren, Jessica. “Site-specific labelling of anti-HER2 ADAPT proteins for molecular imaging applications.” 2016. Web. 17 Sep 2019.
Vancouver:
Lövgren J. Site-specific labelling of anti-HER2 ADAPT proteins for molecular imaging applications. [Internet] [Thesis]. Uppsala University; 2016. [cited 2019 Sep 17].
Available from: http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-302905.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Lövgren J. Site-specific labelling of anti-HER2 ADAPT proteins for molecular imaging applications. [Thesis]. Uppsala University; 2016. Available from: http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-302905
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
University of Tennessee – Knoxville
10.
Williamson, Andrew Carl.
SOLID TARGET SYSTEM FOR USE ON AN 11 MeV CYCLOTRON.
Degree: MS, Mechanical Engineering, 2010, University of Tennessee – Knoxville
URL: https://trace.tennessee.edu/utk_gradthes/843
► Molecular imaging is becoming an important contributor to the development of personalized medicine. Positron Emission Tomography (PET) is a technology that enables molecular imaging by…
(more)
▼ Molecular imaging is becoming an important contributor to the development of personalized medicine. Positron Emission Tomography (PET) is a technology that enables
molecular imaging by allowing a physician to detect and map the location of various physiological processes. The purpose of this work is to design, fabricate and test a mechanism that would make the production of the PET isotope, copper-64 practical for both researchers and commercial suppliers. In order to have the maximum usefulness, the design needs to fit and operate within several constraints. A one dimensional thermal analysis indicated that operation of the system under existing cooling conditions would be a reasonable solution. Based on the design specifications, a detailed design was completed and fabricated. The design was functionally and operationally tested with the performance meeting expectations. The design was utilized to produce copper-64 isotope with a typical one hour bombardment producing 30 mCi of isotope. The design could be optimized if future isotope demand exceeded current production capacity or if research required the production of other radioisotopes with varying thermal characteristics.
Advisors/Committee Members: William R. Hamel, Kivanc Ekici, G.V. Smith.
Subjects/Keywords: Molecular Imaging; cyclotron; PET; Other Mechanical Engineering
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APA (6th Edition):
Williamson, A. C. (2010). SOLID TARGET SYSTEM FOR USE ON AN 11 MeV CYCLOTRON. (Thesis). University of Tennessee – Knoxville. Retrieved from https://trace.tennessee.edu/utk_gradthes/843
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Williamson, Andrew Carl. “SOLID TARGET SYSTEM FOR USE ON AN 11 MeV CYCLOTRON.” 2010. Thesis, University of Tennessee – Knoxville. Accessed September 17, 2019.
https://trace.tennessee.edu/utk_gradthes/843.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Williamson, Andrew Carl. “SOLID TARGET SYSTEM FOR USE ON AN 11 MeV CYCLOTRON.” 2010. Web. 17 Sep 2019.
Vancouver:
Williamson AC. SOLID TARGET SYSTEM FOR USE ON AN 11 MeV CYCLOTRON. [Internet] [Thesis]. University of Tennessee – Knoxville; 2010. [cited 2019 Sep 17].
Available from: https://trace.tennessee.edu/utk_gradthes/843.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Williamson AC. SOLID TARGET SYSTEM FOR USE ON AN 11 MeV CYCLOTRON. [Thesis]. University of Tennessee – Knoxville; 2010. Available from: https://trace.tennessee.edu/utk_gradthes/843
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
University of Illinois – Urbana-Champaign
11.
Li, Hao.
Development of small molecule probes for fluorescence and photoacoustic molecular imaging.
Degree: MS, Chemistry, 2017, University of Illinois – Urbana-Champaign
URL: http://hdl.handle.net/2142/97260
► Molecular imaging enables the visualization, characterization and quantification of biochemical processes taking place at the molecular levels within intact living subjects. As such, it has…
(more)
▼ Molecular imaging enables the visualization, characterization and quantification of biochemical processes taking place at the
molecular levels within intact living subjects. As such, it has become an indispensable tool for both basic research and biomedical applications. In particular, fluorescence
imaging allows us to study
molecular processes at the cellular level in real time because of its excellent sensitivity, high resolution and non-invasive nature. The use of reaction-based small-molecule fluorescent probes, a class of probes that exhibit differential fluorescence signals upon reacting with their intended target, has further rendered fluorescence
imaging with good selectivity and higher sensitivity. Such reaction-based small-molecule fluorescent probes is often comprised of at least two components: a dye capable of generating a large fluorescence signal upon excitation and a target-specific reactive handle. The selection of both components are important as the dye often determines the spacial resolution as well as biocompatibility, whereas the reactive handle dictates selectivity and sensitivity. As such, finding new dye platforms as well as new reactive handles are essential to expanding the repertoire of reaction-based probes for non-invasive real-time fluorescence
imaging. In Chapter 1, I will describe the development of a formaldehyde (FA)-responsive small-molecule fluorescent probe, formaldehyde probe 1 (FP1). Specifically, we utilized a FA-specific biorthogonal chemical transformation to render FP1 FA-specific. Moreover, we also developed a neutral, red-shifted fluorophore platform with exceptional photostability to enable the real-time tracking of FA and in the hope of studying the long-term effect of FA in living systems.
While fluorescence
imaging excels at studying cellular systems, its usage in vivo is challenging due to the limited
imaging depth up to 1 mm as a result of light scatting. In other word, if the
imaging target resides deeper than 1 mm, the outcoming fluorescence will scatter and hence erodes the resolution. As the thickness of the human skin varies from 0.5 to 4 mm depending on different parts of the human body, most optical methods including fluorescence
imaging can only be applied ex-vivo or to study superficial subjects such as skin. On the other hand, photoacoustic
imaging (PAI), an
imaging technique based on the detection of ultrasonic waves generated by light absorption, circumvents this problem because it detects ultrasonic waves which scatters 3000 times less than light. As a result, PAI can reach several centimeters of depth penetration while retaining high special resolution. When coupled with small-molecule stimuli-responsive photoacoustic probes that yield a different PA signal upon activation by the
molecular target, PAI becomes a versatile
molecular imaging technique that holds great promise for both animal models studies and human diagnostics. In Chapter 2, I will describe the development of two copper(II)-responsive small-molecule photoacoustic probes APC-1 and…
Advisors/Committee Members: Chan, Jefferson (advisor).
Subjects/Keywords: Molecular imaging; Fluorescence and photoacoustic; Activatable probes
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APA (6th Edition):
Li, H. (2017). Development of small molecule probes for fluorescence and photoacoustic molecular imaging. (Thesis). University of Illinois – Urbana-Champaign. Retrieved from http://hdl.handle.net/2142/97260
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Li, Hao. “Development of small molecule probes for fluorescence and photoacoustic molecular imaging.” 2017. Thesis, University of Illinois – Urbana-Champaign. Accessed September 17, 2019.
http://hdl.handle.net/2142/97260.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Li, Hao. “Development of small molecule probes for fluorescence and photoacoustic molecular imaging.” 2017. Web. 17 Sep 2019.
Vancouver:
Li H. Development of small molecule probes for fluorescence and photoacoustic molecular imaging. [Internet] [Thesis]. University of Illinois – Urbana-Champaign; 2017. [cited 2019 Sep 17].
Available from: http://hdl.handle.net/2142/97260.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Li H. Development of small molecule probes for fluorescence and photoacoustic molecular imaging. [Thesis]. University of Illinois – Urbana-Champaign; 2017. Available from: http://hdl.handle.net/2142/97260
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Rice University
12.
Yang, Dalu.
Identifying and Predicting Molecular Signatures in Glioblastoma Using Imaging-Derived Phenotypic Traits.
Degree: MS, Engineering, 2017, Rice University
URL: http://hdl.handle.net/1911/96026
► This thesis addresses the problem of linking molecular status of glioblastoma patients with imaging-derived phenotypic traits. Glioblastoma (GBM) is the most common and aggressive type…
(more)
▼ This thesis addresses the problem of linking
molecular status of glioblastoma patients with
imaging-derived phenotypic traits. Glioblastoma (GBM) is the most common and aggressive type of malignant brain tumor, with a median survival of only 12-15 months. Due to GBM’s complex heterogeneity in gene expression, the responses to current treatment strategy varies considerably among different patients. There is an urgent need for a deeper understanding of tumor biology and alternative personalized therapeutic intervention. Magnetic Resonance
Imaging (MRI) and histologic images are routinely used for GBM diagnosis. A natural question to ask is that if the phenotypic tumor traits from these images can be linked to tumor
molecular signatures. In this thesis, we explore the
imaging-genomic relationship in GBM via three approaches. The first approach aims to find texture features extracted from the MRI images that best discriminate GBM
molecular subtypes. The second approach aims to find gene networks that determines the radiologically-defined tumor sub-compartment volumes. The third approach aims to quantify GBM histologic hallmarks and correlate them with biological pathway activities. Our study shows that linking
imaging traits with tumor
molecular status can lead to discoveries that have potential clinical relevance and provide biological insight.
Advisors/Committee Members: Rao, Arvind (advisor), Veeraraghavan, Ashok (advisor).
Subjects/Keywords: imaging-genomics; glioblastoma; molecular programs; computer vision
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APA (6th Edition):
Yang, D. (2017). Identifying and Predicting Molecular Signatures in Glioblastoma Using Imaging-Derived Phenotypic Traits. (Masters Thesis). Rice University. Retrieved from http://hdl.handle.net/1911/96026
Chicago Manual of Style (16th Edition):
Yang, Dalu. “Identifying and Predicting Molecular Signatures in Glioblastoma Using Imaging-Derived Phenotypic Traits.” 2017. Masters Thesis, Rice University. Accessed September 17, 2019.
http://hdl.handle.net/1911/96026.
MLA Handbook (7th Edition):
Yang, Dalu. “Identifying and Predicting Molecular Signatures in Glioblastoma Using Imaging-Derived Phenotypic Traits.” 2017. Web. 17 Sep 2019.
Vancouver:
Yang D. Identifying and Predicting Molecular Signatures in Glioblastoma Using Imaging-Derived Phenotypic Traits. [Internet] [Masters thesis]. Rice University; 2017. [cited 2019 Sep 17].
Available from: http://hdl.handle.net/1911/96026.
Council of Science Editors:
Yang D. Identifying and Predicting Molecular Signatures in Glioblastoma Using Imaging-Derived Phenotypic Traits. [Masters Thesis]. Rice University; 2017. Available from: http://hdl.handle.net/1911/96026
University of Texas Southwestern Medical Center
13.
Lin, Mai.
Molecular Imaging of αvβ6–Positive Tumors and Pancreatic β-Cell Mass by Radiolabeled Peptides.
Degree: 2011, University of Texas Southwestern Medical Center
URL: http://hdl.handle.net/2152.5/856
► Conventional diagnostic methodologies of lung cancer and diabetes are limited by sensitivity and specificity. In consequence, patients usually get diagnosed when the symptoms appear and…
(more)
▼ Conventional diagnostic methodologies of lung cancer and diabetes are limited by sensitivity and specificity. In consequence, patients usually get diagnosed when the symptoms appear and the diseases are at the advanced stages. As the expressions of the avß6 integrin and glucagon-like peptide-1 receptor (GLP-1R) are highly related to aggressive tumor phenotypes and functional pancreatic ß-cells, this work has been set to develop peptide-based radiotracers that can specifically bind to avß6 or GLP-1R for noninvasively monitoring the progression of lung cancer and diabetes.
By phage display, a peptide sequence that specific binds to avß6 was identified. In the evaluation of its truncated forms with similar binding affinity, a polyethylene glycol chain (PEG11) was inserted to the C-terminus and a bifunctional chelator (DOTA) was conjugated to either end of the peptides. The conjugates were labeled with 111In (t1/2: 2.8 d) under mild conditions with the highest achievable specific activity of 1.15 × 104 MBq/µmol. The in vivo evaluation was performed in a lung adenocarcinoma xenograft mouse model. Of the six conjugates, 10PD showed the best tissue contrast of the H2009 (avß6+) tumor. However, it also yielded to have the highest renal accumulation. The high kidney uptake of 10PD was found to be alleviated by conjugating DOTA at the N-terminus or reducing the peptide net charges.
To evaluate GLP-1-based radiotracers for
imaging pancreatic ß-cell mass (BCM), GLP-1, [D-Ala8]GLP-1, two bicyclic GLP-1 analogs (EM2196 and EM2198), and exendin-4 were synthesized and compared for their biological properties. All peptide constructs were tagged with an 6-aminohexanoic linker (Ahx) followed by DOTA conjugation at C-terminus and labeled with 64Cu (t1/2: 12.7 h). The specific activity of the labeled peptide conjugates was up to 1.0 × 106 MBq/µmol with radiochemical purity over 97%. Compared to GLP-1, [D-Ala8]GLP-1 revealed strong resistance against DPP-IV. In addition, EM2198 demonstrated high stability against NEP 24.11 presumably by the shielding effects from the two lactam bridges. All peptide conjugates were highly selective to the GLP-1R with the IC50 values in 0.1-0.4 nM. However, only 64Cu-EM2198 showed clear pancreas area on the microPET/CT studies. The signal of 64Cu-EM2198 from the pancreas was confirmed by the ex vivo
imaging scans. The potential of 64Cu-EM2198 for
imaging BCM was further supported by co-injecting a blocking dose of unlabeled exendin-4 and performing
imaging studies in the STZ-treated diabetic mice.
Advisors/Committee Members: Sun, Xiankai.
Subjects/Keywords: Diabetes Mellitus; Lung Neoplasms; Integrins; Molecular Imaging
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APA ·
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Export
to Zotero / EndNote / Reference
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APA (6th Edition):
Lin, M. (2011). Molecular Imaging of αvβ6–Positive Tumors and Pancreatic β-Cell Mass by Radiolabeled Peptides. (Thesis). University of Texas Southwestern Medical Center. Retrieved from http://hdl.handle.net/2152.5/856
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Lin, Mai. “Molecular Imaging of αvβ6–Positive Tumors and Pancreatic β-Cell Mass by Radiolabeled Peptides.” 2011. Thesis, University of Texas Southwestern Medical Center. Accessed September 17, 2019.
http://hdl.handle.net/2152.5/856.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Lin, Mai. “Molecular Imaging of αvβ6–Positive Tumors and Pancreatic β-Cell Mass by Radiolabeled Peptides.” 2011. Web. 17 Sep 2019.
Vancouver:
Lin M. Molecular Imaging of αvβ6–Positive Tumors and Pancreatic β-Cell Mass by Radiolabeled Peptides. [Internet] [Thesis]. University of Texas Southwestern Medical Center; 2011. [cited 2019 Sep 17].
Available from: http://hdl.handle.net/2152.5/856.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Lin M. Molecular Imaging of αvβ6–Positive Tumors and Pancreatic β-Cell Mass by Radiolabeled Peptides. [Thesis]. University of Texas Southwestern Medical Center; 2011. Available from: http://hdl.handle.net/2152.5/856
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
University of Arizona
14.
Sinharay, Sanhita.
Development and Application of CatalyCEST MRI Contrast Agents for the Study of Enzyme Activities in Tumor Models
.
Degree: 2016, University of Arizona
URL: http://hdl.handle.net/10150/612945
► The in vivo detection of enzyme activity is a significant biomarker in tumorigenesis. Assessment of enzyme activity relative to enzyme concentration can serve as quite…
(more)
▼ The in vivo detection of enzyme activity is a significant biomarker in tumorigenesis. Assessment of enzyme activity relative to enzyme concentration can serve as quite an accurate measurement of several disease states. Chemical Exchange Saturation Transfer (CEST) MRI is a non-invasive
imaging technique that can be used to evaluate enzyme activity. Compared to other contrast agents CEST MRI agents have a slower chemical exchange rate and thus have greater specificity for detecting the intended biomarker. Chapter 1 provides an overview of the advances made in the field of
molecular imaging for detection of cancer biomarkers. The
molecular mechanism of each technique is explained with specific examples and advantages as well as disadvantages of each technique. Chapter 2 investigates the specific example of detection of an enzyme, γ-glutamyl transferase (GGT) in ovarian cancer tumor models using a catalyCEST MRI contrast agent. This chapter discusses the step-by step evaluation of the non-metallic contrast agent, from synthesis to evaluation of its catalytic efficiency with Michaelis Menten kinetics studies and finally in vivo GGT detection in ovarian tumor models of OVCAR-8 and OVCAR-3. Chapter 3 investigates the enzyme, Kallikrein-6 and its detection in HCT116 colon cancer tumor model. In addition to enzyme detection, enzyme inhibition using Antithrombin III inhibitor has also been explored within in vitro media and in vivo HCT116 tumor model. Chapter 4 introduces the catalyCEST agent for detection of sulfatase enzyme. This chapter discusses the synthesis of this agent and its ability to detect sulfatase in bacterial cell suspension and mammalian cell suspension. These examples portray catalyCEST MRI as a platform technology for enzyme activity detection. Finally in Chapter 5 future ideas have been proposed to improve the in vivo detection and broaden the applications of catalyCEST MRI in the field of enzyme studies.
Advisors/Committee Members: Pagel, Mark D (advisor), Kuo, Philip H. (committeemember), Glass, Richard S. (committeemember), Mash, Eugene A. (committeemember), Pagel, Mark D. (committeemember).
Subjects/Keywords: Enzymes;
Molecular Imaging;
Chemistry;
CEST MRI
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APA (6th Edition):
Sinharay, S. (2016). Development and Application of CatalyCEST MRI Contrast Agents for the Study of Enzyme Activities in Tumor Models
. (Doctoral Dissertation). University of Arizona. Retrieved from http://hdl.handle.net/10150/612945
Chicago Manual of Style (16th Edition):
Sinharay, Sanhita. “Development and Application of CatalyCEST MRI Contrast Agents for the Study of Enzyme Activities in Tumor Models
.” 2016. Doctoral Dissertation, University of Arizona. Accessed September 17, 2019.
http://hdl.handle.net/10150/612945.
MLA Handbook (7th Edition):
Sinharay, Sanhita. “Development and Application of CatalyCEST MRI Contrast Agents for the Study of Enzyme Activities in Tumor Models
.” 2016. Web. 17 Sep 2019.
Vancouver:
Sinharay S. Development and Application of CatalyCEST MRI Contrast Agents for the Study of Enzyme Activities in Tumor Models
. [Internet] [Doctoral dissertation]. University of Arizona; 2016. [cited 2019 Sep 17].
Available from: http://hdl.handle.net/10150/612945.
Council of Science Editors:
Sinharay S. Development and Application of CatalyCEST MRI Contrast Agents for the Study of Enzyme Activities in Tumor Models
. [Doctoral Dissertation]. University of Arizona; 2016. Available from: http://hdl.handle.net/10150/612945
University of Waterloo
15.
Ramadhan, Ali.
Molecular movies and geometry reconstruction using Coulomb explosion imaging.
Degree: 2017, University of Waterloo
URL: http://hdl.handle.net/10012/12190
► Coulomb explosion imaging is a technique of imaging the structure of small molecules in the gas phase and their ultrafast dynamics by inducing the rapid…
(more)
▼ Coulomb explosion imaging is a technique of imaging the structure of small molecules in the gas phase and their ultrafast dynamics by inducing the rapid ionization and dissociation of the molecule into its constituent atomic fragments. The momentum vectors of the atomic fragments facilitate the retrieval of the molecule's structure, however, few attempts at geometry reconstruction appear in the published literature, whose vague methodology casts serious doubts on the geometry reconstructions that have been performed, and motivating the need for an investigation into the feasibility of geometry reconstruction. We develop a method for the fast and precise reconstruction of triatomic molecular geometries by casting the task as a nonlinear constrained optimization problem. We use this method to investigate the uncertainty in geometry reconstructions as a function of measurement uncertainty as well as the existence and nature of multiple solutions to the geometry reconstruction problem. We map out the conditions under which molecular geometries may be accurately reconstructed and propose a framework for reconstructing geometries, and therefore producing molecular movies using Coulomb explosion imaging.
Subjects/Keywords: Coulomb explosion imaging; molecular imaging; molecular movies; molecular geometry reconstruction; ultrafast; molecular dynamics; ultrashort laser; lookup table; mathematical optimization; optimization
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Manager
APA (6th Edition):
Ramadhan, A. (2017). Molecular movies and geometry reconstruction using Coulomb explosion imaging. (Thesis). University of Waterloo. Retrieved from http://hdl.handle.net/10012/12190
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Ramadhan, Ali. “Molecular movies and geometry reconstruction using Coulomb explosion imaging.” 2017. Thesis, University of Waterloo. Accessed September 17, 2019.
http://hdl.handle.net/10012/12190.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Ramadhan, Ali. “Molecular movies and geometry reconstruction using Coulomb explosion imaging.” 2017. Web. 17 Sep 2019.
Vancouver:
Ramadhan A. Molecular movies and geometry reconstruction using Coulomb explosion imaging. [Internet] [Thesis]. University of Waterloo; 2017. [cited 2019 Sep 17].
Available from: http://hdl.handle.net/10012/12190.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Ramadhan A. Molecular movies and geometry reconstruction using Coulomb explosion imaging. [Thesis]. University of Waterloo; 2017. Available from: http://hdl.handle.net/10012/12190
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Washington University in St. Louis
16.
Solomon, Metasebya.
Video-Rate Fluorescence Molecular Tomography for Hand-held and Multimodal Molecular Imaging.
Degree: PhD, Biomedical Engineering, 2012, Washington University in St. Louis
URL: https://openscholarship.wustl.edu/etd/1019
► In the United States, cancer is the second leading cause of death following heart disease. Although, a variety of treatment regimens are available, cancer…
(more)
▼ In the United States, cancer is the second leading cause of death following heart disease. Although, a variety of treatment regimens are available, cancer management is complicated by the complexity of the disease and the variability, between people, of disease progression and response to therapy. Therefore, advancements in the methods and technologies for cancer diagnosis, prognosis and therapeutic monitoring are critical to improving the treatment of cancer patients. The development of improved
imaging methods for early diagnosis of cancer and of near real-time monitoring of tumor response to therapy may improve outcomes as well as the quality of life of cancer patients. In the last decade,
imaging methods including ultrasound, computed tomography: CT), magnetic resonance
imaging: MRI), single photon emission computed tomography: SPECT), and positron emission tomography: PET), have revolutionized oncology. More recently optical techniques, that have access to unique
molecular reporting strategies and functional contrasts, show promise for oncologic
imaging This dissertation focuses on the development and optimization of a fiber-based, video-rate fluorescence
molecular tomography: FMT) instrument. Concurrent acquisition of fluorescence and reference signals allowed the efficient generation of ratio-metric data for 3D image reconstruction. Accurate depth localization and high sensitivity to fluorescent targets were established to depths of >10 mm. In vivo accumulation of indocyanine green dye was imaged in the region of the sentinel lymph node: SLN) following intradermal injection into the forepaw of rats. These results suggest that video-rate FMT has potential as a clinical tool for noninvasive mapping of SLN. Spatial and temporal co-registration of nuclear and optical images can enable the fusion of the information from these complementary
molecular imaging modalities. A critical challenge is in integrating the optical and nuclear
imaging hardware. Flexible fiber-based FMT systems provide a viable solution. The various
imaging bore sizes of small animal nuclear
imaging systems can potentially accommodate the FMT fiber
imaging arrays. In addition FMT
imaging facilitates co-registering the nuclear and optical contrasts in time. In this dissertation, the feasibility of integrating the fiber-based, video-rate FMT system with a commercial preclinical NanoSPECT/CT platform was established. Feasibility of in vivo
imaging is demonstrated by tracking a monomolecular multimodal-
imaging agent: MOMIA) during transport from the forepaw to the axillary lymph nodes region of a rat. These co-registered FMT/SPECT/CT
imaging results with MOMIAs may facilitate the development of the next generation preclinical and clinical multimodal optical-nuclear platforms for a broad array of
imaging applications, and help elucidate the underlying biological processes relevant to cancer diagnosis and therapy monitoring. Finally, I demonstrated that video-rate FMT is sufficiently fast to enable
imaging of cardiac,…
Advisors/Committee Members: Samuel Achilefu.
Subjects/Keywords: Diffuse Optical Tomography; Fluorescence Molecular Tomography; Lymph Node Imaging; Multimodal Imaging; Small Animal Imaging
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APA (6th Edition):
Solomon, M. (2012). Video-Rate Fluorescence Molecular Tomography for Hand-held and Multimodal Molecular Imaging. (Doctoral Dissertation). Washington University in St. Louis. Retrieved from https://openscholarship.wustl.edu/etd/1019
Chicago Manual of Style (16th Edition):
Solomon, Metasebya. “Video-Rate Fluorescence Molecular Tomography for Hand-held and Multimodal Molecular Imaging.” 2012. Doctoral Dissertation, Washington University in St. Louis. Accessed September 17, 2019.
https://openscholarship.wustl.edu/etd/1019.
MLA Handbook (7th Edition):
Solomon, Metasebya. “Video-Rate Fluorescence Molecular Tomography for Hand-held and Multimodal Molecular Imaging.” 2012. Web. 17 Sep 2019.
Vancouver:
Solomon M. Video-Rate Fluorescence Molecular Tomography for Hand-held and Multimodal Molecular Imaging. [Internet] [Doctoral dissertation]. Washington University in St. Louis; 2012. [cited 2019 Sep 17].
Available from: https://openscholarship.wustl.edu/etd/1019.
Council of Science Editors:
Solomon M. Video-Rate Fluorescence Molecular Tomography for Hand-held and Multimodal Molecular Imaging. [Doctoral Dissertation]. Washington University in St. Louis; 2012. Available from: https://openscholarship.wustl.edu/etd/1019
University of Texas – Austin
17.
Luke, Geoffrey Patrick.
Functional and molecular photoacoustic imaging for the detection of lymph node metastasis.
Degree: PhD, Electrical and Computer Engineering, 2013, University of Texas – Austin
URL: http://hdl.handle.net/2152/28713
► Accurate detection of the spread of cancer is critical for planning the best treatment strategy for a patient. Currently, an invasive sentinel lymph node biopsy…
(more)
▼ Accurate detection of the spread of cancer is critical for planning the best treatment strategy for a patient. Currently, an invasive sentinel lymph node biopsy is commonly used to detect metastases after a primary tumor is detected. This procedure results in patient morbidity, requires weeks of waiting, and is prone to sampling error. This dissertation presents new developments in an emerging biomedical
imaging modality – photoacoustic
imaging – and their application to improving the detection of metastases in the lymphatic system in a metastatic mouse model of squamous cell carcinoma of the oral cavity. Label-free spectroscopic photoacoustic
imaging is demonstrated to detect hypoxia that results from the development of sub-millimeter cancer foci in the lymph node. In order to improve the sensitivity to micrometastases, molecularly-activated plasmonic nanosensers which are targeted to the epidermal growth factor receptor are introduced. The nanosensors are demonstrated to detect metastases consisting of only a few tens of cells. Improvements to spectroscopic photoacoustic
imaging are then demonstrated by selecting
imaging wavelengths based on the spectral properties of the optical absorbers. Finally, a new contrast agent – silica-coated gold nanoplates – are used to map the sentinel lymph node with high contrast. The final result is a set of tools that can be used to noninvasively detect micrometastases and improve
molecular photoacoustic
imaging.
Advisors/Committee Members: Emelianov, Stanislav Y. (advisor).
Subjects/Keywords: Photoacoustic imaging; Molecular imaging; Sentinel lymph node; Ultrasound imaging; Nanoparticles; Image processing; Spectroscopy
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APA (6th Edition):
Luke, G. P. (2013). Functional and molecular photoacoustic imaging for the detection of lymph node metastasis. (Doctoral Dissertation). University of Texas – Austin. Retrieved from http://hdl.handle.net/2152/28713
Chicago Manual of Style (16th Edition):
Luke, Geoffrey Patrick. “Functional and molecular photoacoustic imaging for the detection of lymph node metastasis.” 2013. Doctoral Dissertation, University of Texas – Austin. Accessed September 17, 2019.
http://hdl.handle.net/2152/28713.
MLA Handbook (7th Edition):
Luke, Geoffrey Patrick. “Functional and molecular photoacoustic imaging for the detection of lymph node metastasis.” 2013. Web. 17 Sep 2019.
Vancouver:
Luke GP. Functional and molecular photoacoustic imaging for the detection of lymph node metastasis. [Internet] [Doctoral dissertation]. University of Texas – Austin; 2013. [cited 2019 Sep 17].
Available from: http://hdl.handle.net/2152/28713.
Council of Science Editors:
Luke GP. Functional and molecular photoacoustic imaging for the detection of lymph node metastasis. [Doctoral Dissertation]. University of Texas – Austin; 2013. Available from: http://hdl.handle.net/2152/28713
McMaster University
18.
Tao, Ashley T.
Advanced Methods in Molecular Breast Imaging.
Degree: PhD, 2016, McMaster University
URL: http://hdl.handle.net/11375/19900
► Molecular breast imaging (MBI) is a relatively new clinical breast imaging modality, which has the potential to have a significant impact in breast cancer screening…
(more)
▼ Molecular breast imaging (MBI) is a relatively new clinical breast imaging modality, which has the potential to have a significant impact in breast cancer screening and perioperative breast imaging for women with high risk factors for developing breast cancer. Two objectives were proposed in this thesis to increase the use of MBI. First, a magnetic resonance (MR)-compatible gamma camera was developed for combined molecular/MR breast imaging. MBI is a functional imaging technique with high specificity and sensitivity but could benefit from the addition of anatomical information from breast MRI for lesion localization, cancer staging, treatment planning and monitoring. A small area (8cm x 8cm) cadmium zinc telluride (CZT) based gamma camera was developed and tested for MR compatibility in both sequential and simultaneous imaging conditions. Results indicated that the gamma camera was minimally affected during both sequential and simultaneous imaging with a gradient echo (GRE) and spoiled gradient echo (GRE) sequence. Signal to noise ratio (SNR) degradation was observed in the MR images but no geometric distortions were observed. Simultaneous imaging is feasible, but a reassessment of the RF shielding would be required to minimize the noise contribution degrading image quality. Second, backscatter photons were investigated as a potential dose reduction technique for MBI. While the effective dose from MBI is relatively low in comparison to other nuclear medicine procedures, the dose is considered high in relation to mammography and in order to increase acceptance as an alternative breast imaging method, dose reduction is an important objective. Backscatter photons have the same spatial information as primary photons but are typically discarded along with other scattered photons. A scatter compensation method called the triple energy window (TEW) was used to extract backscatter photons from the Compton scattering spectrum and added to the primary photons, increasing count sensitivity by 6%. The noise level matched the increase in contrast leading to negligible change in lesion contrast to noise ratio (CNR). Dose reduction is not justified with this particular technique because of the elevated noise level, but the use of backcsatter photons show potential with improved contrast.
Dissertation
Doctor of Philosophy (PhD)
Advisors/Committee Members: Farncombe, Troy H., Medical Physics.
Subjects/Keywords: Molecular breast imaging; MRI; Nuclear medicine; Dual-modality imaging; Backscatter
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APA (6th Edition):
Tao, A. T. (2016). Advanced Methods in Molecular Breast Imaging. (Doctoral Dissertation). McMaster University. Retrieved from http://hdl.handle.net/11375/19900
Chicago Manual of Style (16th Edition):
Tao, Ashley T. “Advanced Methods in Molecular Breast Imaging.” 2016. Doctoral Dissertation, McMaster University. Accessed September 17, 2019.
http://hdl.handle.net/11375/19900.
MLA Handbook (7th Edition):
Tao, Ashley T. “Advanced Methods in Molecular Breast Imaging.” 2016. Web. 17 Sep 2019.
Vancouver:
Tao AT. Advanced Methods in Molecular Breast Imaging. [Internet] [Doctoral dissertation]. McMaster University; 2016. [cited 2019 Sep 17].
Available from: http://hdl.handle.net/11375/19900.
Council of Science Editors:
Tao AT. Advanced Methods in Molecular Breast Imaging. [Doctoral Dissertation]. McMaster University; 2016. Available from: http://hdl.handle.net/11375/19900
McMaster University
19.
Zlitni, Aimen.
The Development and Evaluation of Multi-Modal Microbubbles and New Strategies for Targeted Ultrasound, Nuclear and Optical Imaging.
Degree: PhD, 2016, McMaster University
URL: http://hdl.handle.net/11375/19578
► Gas filled microbubbles (MBs) stabilized by a shell (e.g. lipids) are commonly used as ultrasound (US) contrast agents. Attaching biomolecules to the surface of MBs…
(more)
▼ Gas filled microbubbles (MBs) stabilized by a shell (e.g. lipids) are commonly used as ultrasound (US) contrast agents. Attaching biomolecules to the surface of MBs allows for molecular US imaging of various diseases. With the increased interest in targeted US imaging, new platforms to prepare disease-targeted MBs are necessary. Furthermore, attaching signaling agents to MBs creates multi-modal imaging opportunities, enhancing visualization and quantification of disease biomarkers.
In this thesis, MBs labeled with 99mTc and/or rhodamine dye by taking advantage of the strong interaction between biotin and streptavidin are reported. Radiolabeling of MBs was achieved in good radiochemical yield (~ 30%). 99mTc-labeled MBs were targeted to vascular endothelial growth factor receptor 2 (VEGFR2) using an anti-VEGFR2 antibody and to prostate specific membrane antigen (PSMA) using small-molecule based PSMA inhibitors. In vitro evaluations showed successful binding of MBs to the target while in vivo targeting assessments were unsuccessful.
New strategies to target MBs to the site of interest were then developed through the use of the bioorthogonal reaction between tetrazine (Tz) and trans-cyclooctene (TCO). A biotinylated derivative of Tz was loaded on streptavidin coated MBs to create a Tz-derivatized MB (MBTz). Targeting MBTz to extracellular markers of cancer such as VEGFR2, PSMA and urokinase plasminogen activator receptor (uPAR) in vitro was achieved using TCO-conjugated antibodies. In vivo targeting was successful for VEGFR2 and PSMA, but not uPAR.
Translating the new strategy to other US contrast agents was then investigated. Gas vesicles (GVs) produced in halobacteria were conjugated with TCO using amide-coupling chemistry. A 99mTc-labeled derivative of Tz was loaded on TCO-GVs (RCY= 59%) and their distribution assessed by SPECT/CT imaging and ex vivo tissue counting. Having established a convenient platform to conjugate molecules to GVs and MBs, future work focuses on developing a new generation of human compatible molecular US imaging probes.
Dissertation
Doctor of Philosophy (PhD)
Advisors/Committee Members: Valliant, John, Chemical Biology.
Subjects/Keywords: Multimodal imaging; Molecular ultrasound imaging of cancer; Microbubbles; bioorthogonal chemistry
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APA (6th Edition):
Zlitni, A. (2016). The Development and Evaluation of Multi-Modal Microbubbles and New Strategies for Targeted Ultrasound, Nuclear and Optical Imaging. (Doctoral Dissertation). McMaster University. Retrieved from http://hdl.handle.net/11375/19578
Chicago Manual of Style (16th Edition):
Zlitni, Aimen. “The Development and Evaluation of Multi-Modal Microbubbles and New Strategies for Targeted Ultrasound, Nuclear and Optical Imaging.” 2016. Doctoral Dissertation, McMaster University. Accessed September 17, 2019.
http://hdl.handle.net/11375/19578.
MLA Handbook (7th Edition):
Zlitni, Aimen. “The Development and Evaluation of Multi-Modal Microbubbles and New Strategies for Targeted Ultrasound, Nuclear and Optical Imaging.” 2016. Web. 17 Sep 2019.
Vancouver:
Zlitni A. The Development and Evaluation of Multi-Modal Microbubbles and New Strategies for Targeted Ultrasound, Nuclear and Optical Imaging. [Internet] [Doctoral dissertation]. McMaster University; 2016. [cited 2019 Sep 17].
Available from: http://hdl.handle.net/11375/19578.
Council of Science Editors:
Zlitni A. The Development and Evaluation of Multi-Modal Microbubbles and New Strategies for Targeted Ultrasound, Nuclear and Optical Imaging. [Doctoral Dissertation]. McMaster University; 2016. Available from: http://hdl.handle.net/11375/19578
Delft University of Technology
20.
Ivashchenko, O.
Development and applications of high-performance small-animal SPECT.
Degree: 2017, Delft University of Technology
URL: http://resolver.tudelft.nl/uuid:3fb5d84e-39ee-43e8-87c6-21871900dabb
;
urn:NBN:nl:ui:24-uuid:3fb5d84e-39ee-43e8-87c6-21871900dabb
;
3fb5d84e-39ee-43e8-87c6-21871900dabb
;
urn:isbn:978-94-92516-35-0
;
10.4233/uuid:3fb5d84e-39ee-43e8-87c6-21871900dabb
;
urn:NBN:nl:ui:24-uuid:3fb5d84e-39ee-43e8-87c6-21871900dabb
;
http://resolver.tudelft.nl/uuid:3fb5d84e-39ee-43e8-87c6-21871900dabb
Subjects/Keywords: SPECT; preclinical imaging; molecular imaging
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APA (6th Edition):
Ivashchenko, O. (2017). Development and applications of high-performance small-animal SPECT. (Doctoral Dissertation). Delft University of Technology. Retrieved from http://resolver.tudelft.nl/uuid:3fb5d84e-39ee-43e8-87c6-21871900dabb ; urn:NBN:nl:ui:24-uuid:3fb5d84e-39ee-43e8-87c6-21871900dabb ; 3fb5d84e-39ee-43e8-87c6-21871900dabb ; urn:isbn:978-94-92516-35-0 ; 10.4233/uuid:3fb5d84e-39ee-43e8-87c6-21871900dabb ; urn:NBN:nl:ui:24-uuid:3fb5d84e-39ee-43e8-87c6-21871900dabb ; http://resolver.tudelft.nl/uuid:3fb5d84e-39ee-43e8-87c6-21871900dabb
Chicago Manual of Style (16th Edition):
Ivashchenko, O. “Development and applications of high-performance small-animal SPECT.” 2017. Doctoral Dissertation, Delft University of Technology. Accessed September 17, 2019.
http://resolver.tudelft.nl/uuid:3fb5d84e-39ee-43e8-87c6-21871900dabb ; urn:NBN:nl:ui:24-uuid:3fb5d84e-39ee-43e8-87c6-21871900dabb ; 3fb5d84e-39ee-43e8-87c6-21871900dabb ; urn:isbn:978-94-92516-35-0 ; 10.4233/uuid:3fb5d84e-39ee-43e8-87c6-21871900dabb ; urn:NBN:nl:ui:24-uuid:3fb5d84e-39ee-43e8-87c6-21871900dabb ; http://resolver.tudelft.nl/uuid:3fb5d84e-39ee-43e8-87c6-21871900dabb.
MLA Handbook (7th Edition):
Ivashchenko, O. “Development and applications of high-performance small-animal SPECT.” 2017. Web. 17 Sep 2019.
Vancouver:
Ivashchenko O. Development and applications of high-performance small-animal SPECT. [Internet] [Doctoral dissertation]. Delft University of Technology; 2017. [cited 2019 Sep 17].
Available from: http://resolver.tudelft.nl/uuid:3fb5d84e-39ee-43e8-87c6-21871900dabb ; urn:NBN:nl:ui:24-uuid:3fb5d84e-39ee-43e8-87c6-21871900dabb ; 3fb5d84e-39ee-43e8-87c6-21871900dabb ; urn:isbn:978-94-92516-35-0 ; 10.4233/uuid:3fb5d84e-39ee-43e8-87c6-21871900dabb ; urn:NBN:nl:ui:24-uuid:3fb5d84e-39ee-43e8-87c6-21871900dabb ; http://resolver.tudelft.nl/uuid:3fb5d84e-39ee-43e8-87c6-21871900dabb.
Council of Science Editors:
Ivashchenko O. Development and applications of high-performance small-animal SPECT. [Doctoral Dissertation]. Delft University of Technology; 2017. Available from: http://resolver.tudelft.nl/uuid:3fb5d84e-39ee-43e8-87c6-21871900dabb ; urn:NBN:nl:ui:24-uuid:3fb5d84e-39ee-43e8-87c6-21871900dabb ; 3fb5d84e-39ee-43e8-87c6-21871900dabb ; urn:isbn:978-94-92516-35-0 ; 10.4233/uuid:3fb5d84e-39ee-43e8-87c6-21871900dabb ; urn:NBN:nl:ui:24-uuid:3fb5d84e-39ee-43e8-87c6-21871900dabb ; http://resolver.tudelft.nl/uuid:3fb5d84e-39ee-43e8-87c6-21871900dabb
University of Toronto
21.
Philp, Lauren.
Towards Clinical Translation of the Porphysome: Establishment of Endometrial Cancer Applications and Investigation of Metabolic Differences in Pyrolipid Isomers.
Degree: 2018, University of Toronto
URL: http://hdl.handle.net/1807/91426
► Treatment of endometrial cancer consists of surgery ± adjuvant therapy depending on lymph node involvement determined by lymphadenectomy. Targeting lymphadenectomy to patients with known metastases…
(more)
▼ Treatment of endometrial cancer consists of surgery ± adjuvant therapy depending on lymph node involvement determined by lymphadenectomy. Targeting lymphadenectomy to patients with known metastases would provide diagnostic and therapeutic benefits while reducing morbidity. Porphysomes are self-assembling nanoparticles made from a multifunctional porphyrin-lipid monomer (pyrolipid) which accumulate preferentially in malignant tissue. Pyrolipid is created through an acylation reaction during which isomerization can occur. A VX2 model of endometrial cancer with retroperitoneal metastases was used to investigate the accuracy of pre-operative 64Cu-Porphysome-enabled PET imaging of lymph nodes and the sensitivity / specificity of in-vivo porphyrin-fluorescence image-guided lymphadenectomy. Pharmacokinetic, biodistribution and biodegradation studies were used to determine differences in Sn1 and Sn2 isomeric Porphysomes. This study demonstrated that Porphysomes are a highly sensitive imaging tool to diagnose primary tumour, metastatic lymph nodes and intra-abdominal metastases and that Sn2 pyrolipid is the optimal isomer for clinical translation due to the preferential biodegradation profile.
M.Sc.
Advisors/Committee Members: Bernardini, Marcus Q, Medical Science.
Subjects/Keywords: Endometrial Cancer; Fluorescence Imaging; Lymphadenectomy; Metastasis; Molecular Imaging; Nanoparticle; 0574
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APA (6th Edition):
Philp, L. (2018). Towards Clinical Translation of the Porphysome: Establishment of Endometrial Cancer Applications and Investigation of Metabolic Differences in Pyrolipid Isomers. (Masters Thesis). University of Toronto. Retrieved from http://hdl.handle.net/1807/91426
Chicago Manual of Style (16th Edition):
Philp, Lauren. “Towards Clinical Translation of the Porphysome: Establishment of Endometrial Cancer Applications and Investigation of Metabolic Differences in Pyrolipid Isomers.” 2018. Masters Thesis, University of Toronto. Accessed September 17, 2019.
http://hdl.handle.net/1807/91426.
MLA Handbook (7th Edition):
Philp, Lauren. “Towards Clinical Translation of the Porphysome: Establishment of Endometrial Cancer Applications and Investigation of Metabolic Differences in Pyrolipid Isomers.” 2018. Web. 17 Sep 2019.
Vancouver:
Philp L. Towards Clinical Translation of the Porphysome: Establishment of Endometrial Cancer Applications and Investigation of Metabolic Differences in Pyrolipid Isomers. [Internet] [Masters thesis]. University of Toronto; 2018. [cited 2019 Sep 17].
Available from: http://hdl.handle.net/1807/91426.
Council of Science Editors:
Philp L. Towards Clinical Translation of the Porphysome: Establishment of Endometrial Cancer Applications and Investigation of Metabolic Differences in Pyrolipid Isomers. [Masters Thesis]. University of Toronto; 2018. Available from: http://hdl.handle.net/1807/91426
University of Arizona
22.
Winkler, Amy.
OPTICAL METHODS FOR MOLECULAR SENSING: SUPPLEMENTING IMAGING OF TISSUE MICROSTRUCTURE WITH MOLECULAR INFORMATION
.
Degree: 2010, University of Arizona
URL: http://hdl.handle.net/10150/195176
► More and more researchers and clinicians are looking to molecular sensing to predict how cells will behave, seeking the answers to questions like "will these…
(more)
▼ More and more researchers and clinicians are looking to
molecular sensing to predict how cells will behave, seeking the answers to questions like "will these tumor cells become malignant?" or "how will these cells respond to chemotherapy?" Optical methods are attractive for answering these questions because optical radiation is safer and less expensive than alternative methods, such as CT which uses X-ray radiation, PET/SPECT which use gamma radiation, or MRI which is expensive and only available in a hospital setting. In this dissertation, three distinct optical methods are explored to detect at the
molecular level: optical coherence tomography (OCT), laser-induced fluorescence (LIF), and optical polarimetry. OCT has the capability to simultaneously capture anatomical information as well as
molecular information using targeted contrast agents such as gold nanoshells. LIF is less useful for capturing anatomical information, but it can achieve significantly better
molecular sensitivity with the use of targeted fluorescent dyes. Optical polarimetry has potential to detect the concentration of helical molecules, such as glucose. All of these methods are noninvasive or minimally invasive.The work is organized into four specific aims. The first is the design and implementation of a fast, high resolution, endoscopic OCT system to facilitate minimally invasive mouse colon
imaging. The second aim is to demonstrate the utility of this system for automatically identifying tumor lesions based on tissue microstructure. The third is to demonstrate the use of contrast agents to detect
molecular expression using OCT and LIF. The last aim is to demonstrate a new method based on optical polarimetry for noninvasive glucose sensing.
Advisors/Committee Members: Barton, Jennifer K (advisor), Utzinger, Urs (committeemember), Kostuk, Ray (committeemember).
Subjects/Keywords: fluorescence;
molecular imaging;
optical coherence tomography;
optical imaging;
polarimetry
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APA (6th Edition):
Winkler, A. (2010). OPTICAL METHODS FOR MOLECULAR SENSING: SUPPLEMENTING IMAGING OF TISSUE MICROSTRUCTURE WITH MOLECULAR INFORMATION
. (Doctoral Dissertation). University of Arizona. Retrieved from http://hdl.handle.net/10150/195176
Chicago Manual of Style (16th Edition):
Winkler, Amy. “OPTICAL METHODS FOR MOLECULAR SENSING: SUPPLEMENTING IMAGING OF TISSUE MICROSTRUCTURE WITH MOLECULAR INFORMATION
.” 2010. Doctoral Dissertation, University of Arizona. Accessed September 17, 2019.
http://hdl.handle.net/10150/195176.
MLA Handbook (7th Edition):
Winkler, Amy. “OPTICAL METHODS FOR MOLECULAR SENSING: SUPPLEMENTING IMAGING OF TISSUE MICROSTRUCTURE WITH MOLECULAR INFORMATION
.” 2010. Web. 17 Sep 2019.
Vancouver:
Winkler A. OPTICAL METHODS FOR MOLECULAR SENSING: SUPPLEMENTING IMAGING OF TISSUE MICROSTRUCTURE WITH MOLECULAR INFORMATION
. [Internet] [Doctoral dissertation]. University of Arizona; 2010. [cited 2019 Sep 17].
Available from: http://hdl.handle.net/10150/195176.
Council of Science Editors:
Winkler A. OPTICAL METHODS FOR MOLECULAR SENSING: SUPPLEMENTING IMAGING OF TISSUE MICROSTRUCTURE WITH MOLECULAR INFORMATION
. [Doctoral Dissertation]. University of Arizona; 2010. Available from: http://hdl.handle.net/10150/195176
University of Washington
23.
Kang, Soyoung.
Investigation of biomarker-targeted SERS nanoparticles for multiplexed molecular imaging of fresh tissue specimens.
Degree: PhD, 2018, University of Washington
URL: http://hdl.handle.net/1773/42476
► Biomarker-targeted surface-enhanced Raman scattering (SERS) nanoparticles (NPs) have been explored as a viable option for targeting and imaging multiple cell-surface protein biomarkers of cancer. A…
(more)
▼ Biomarker-targeted surface-enhanced Raman scattering (SERS) nanoparticles (NPs) have been explored as a viable option for targeting and
imaging multiple cell-surface protein biomarkers of cancer. A previously-developed Raman-encoded
molecular imaging (REMI) technique utilizes such targeted NPs for topical application onto excised tissues to enable rapid visualization of a multiplexed panel of cell surface biomarkers at surgical margin surfaces. While it has been demonstrated that REMI may potentially be used to guide tumor-resection procedures, the strategy would benefit from improvements described in this thesis. First, an investigation into channel-compressed spectrometry revealed that up to 64 times fewer spectral channels may be used to accurately demultiplex up to five SERS NP flavors (compared to our previous methods). This strategy offers the potential for improved
imaging speed and/or detection sensitivity with a low-channel count detector in future REMI systems. Next, the complexities in nonspecific accumulation, diffusion, and chemical binding of targeted NPs in fresh tissues were explored in a microscopic investigation quantifying the specific vs. nonspecific accumulation of topically-applied NPs as they diffuse into fresh tissue. The findings from this study led us to hypothesize and later demonstrate that by reducing NP diffusion, nonspecific accumulations of NPs in tissue is reduced, thereby allowing for
molecular imaging of fresh tissue surfaces with higher NP ratios (targeted vs. untargeted), and that the staining can be achieved more rapidly than before (6-min topical application). A third, and final, study is presented, to help establish optimized protocols for the staining and rinsing of fresh tissue specimens for REMI using a mathematical model that incorporates multi-layer diffusion in addition to binding and nonspecific retention compartments. The goal of this final study is for this forward model to ultimately be used to enable quantitative methods of evaluating
molecular expression, which could enable improved assessments of tumor margins (e.g., the use of multi-stage staining/rinsing processes to allow kinetic model fitting of data). In summary, the first two studies enable the design of more rapid
molecular imaging systems, and NP agents with improved sensitivity and contrast, respectively, for rapid
molecular imaging of fresh tissues suitable for intraoperative clinical settings. The mathematical model study is valuable for accurately quantifying biomarker expression levels to potentially increase the sensitivity and specificity of tumor detection at surgical margins.
Advisors/Committee Members: Liu, Jonathan T.C. (advisor).
Subjects/Keywords: molecular imaging; multiplexed molecular imaging; nanoparticles; optics; Raman spectroscopy; surface-enhanced Raman scattering; Medical imaging; Optics; Mechanical engineering; Mechanical engineering
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APA (6th Edition):
Kang, S. (2018). Investigation of biomarker-targeted SERS nanoparticles for multiplexed molecular imaging of fresh tissue specimens. (Doctoral Dissertation). University of Washington. Retrieved from http://hdl.handle.net/1773/42476
Chicago Manual of Style (16th Edition):
Kang, Soyoung. “Investigation of biomarker-targeted SERS nanoparticles for multiplexed molecular imaging of fresh tissue specimens.” 2018. Doctoral Dissertation, University of Washington. Accessed September 17, 2019.
http://hdl.handle.net/1773/42476.
MLA Handbook (7th Edition):
Kang, Soyoung. “Investigation of biomarker-targeted SERS nanoparticles for multiplexed molecular imaging of fresh tissue specimens.” 2018. Web. 17 Sep 2019.
Vancouver:
Kang S. Investigation of biomarker-targeted SERS nanoparticles for multiplexed molecular imaging of fresh tissue specimens. [Internet] [Doctoral dissertation]. University of Washington; 2018. [cited 2019 Sep 17].
Available from: http://hdl.handle.net/1773/42476.
Council of Science Editors:
Kang S. Investigation of biomarker-targeted SERS nanoparticles for multiplexed molecular imaging of fresh tissue specimens. [Doctoral Dissertation]. University of Washington; 2018. Available from: http://hdl.handle.net/1773/42476
University of Toronto
24.
Burgess, Allysa Jane Laura.
Topical Molecular Beacons for In Vivo Image-guided Resection of Oral Carcinoma.
Degree: 2014, University of Toronto
URL: http://hdl.handle.net/1807/67951
► Oral carcinoma has become a major health problem, with nearly 300,000 people diagnosed worldwide annually. The 5-year survival rate is as low as 30%, mainly…
(more)
▼ Oral carcinoma has become a major health problem, with nearly 300,000 people diagnosed worldwide annually. The 5-year survival rate is as low as 30%, mainly attributed to poor delineation of lesions. Optical imaging approaches to identify oral carcinoma tissue during surgery are currently in trial. While decreased recurrence rates are shown, high rates of false positives occur. Expanding upon this, a molecular beacon strategy for oral carcinoma delineation was devised.The selected MMP molecular beacon consists of a fluorophore conjugated to a quencher via a disease-specific linker, activated by MMPs. The activated beacon becomes fluorescent, guiding resection. MMPs have been associated with oral tumors and several members as highly upregulated in oral carcinoma, making them an ideal target.I investigated, in vivo, the utility of this MMP-cleavable beacon in targeting oral carcinoma. Here I demonstrate its high tumor specificity and potential for integration into the clinic to improve patient outcome.
M.Sc.
Advisors/Committee Members: Zheng, Gang, Medical Biophysics.
Subjects/Keywords: matrix metalloproteinases; molecular beacons; molecular imaging; oral carcinoma; 0786
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APA (6th Edition):
Burgess, A. J. L. (2014). Topical Molecular Beacons for In Vivo Image-guided Resection of Oral Carcinoma. (Masters Thesis). University of Toronto. Retrieved from http://hdl.handle.net/1807/67951
Chicago Manual of Style (16th Edition):
Burgess, Allysa Jane Laura. “Topical Molecular Beacons for In Vivo Image-guided Resection of Oral Carcinoma.” 2014. Masters Thesis, University of Toronto. Accessed September 17, 2019.
http://hdl.handle.net/1807/67951.
MLA Handbook (7th Edition):
Burgess, Allysa Jane Laura. “Topical Molecular Beacons for In Vivo Image-guided Resection of Oral Carcinoma.” 2014. Web. 17 Sep 2019.
Vancouver:
Burgess AJL. Topical Molecular Beacons for In Vivo Image-guided Resection of Oral Carcinoma. [Internet] [Masters thesis]. University of Toronto; 2014. [cited 2019 Sep 17].
Available from: http://hdl.handle.net/1807/67951.
Council of Science Editors:
Burgess AJL. Topical Molecular Beacons for In Vivo Image-guided Resection of Oral Carcinoma. [Masters Thesis]. University of Toronto; 2014. Available from: http://hdl.handle.net/1807/67951
University of California – San Francisco
25.
Darragh, Molly Rose.
Targeting the Active Serine Protease MT-SP1 for Tumor Detection <italics>in vivo</italics>.
Degree: Biophysics, 2010, University of California – San Francisco
URL: http://www.escholarship.org/uc/item/69q0228b
► Cancer is a disease which develops as the result of many negative changes in the biology of otherwise healthy tissue. Non-invasive imaging of aberrant, tumor-associated…
(more)
▼ Cancer is a disease which develops as the result of many negative changes in the biology of otherwise healthy tissue. Non-invasive imaging of aberrant, tumor-associated molecules or processes can be used to isolate and characterize the malignancy, but first one must identify those characteristics which differentiate normal and cancerous tissues. Proteases are associated with cancer growth and progression and are often considered in the search for biomarkers to be used in tumor detection and evaluation. Membrane-Type Serine Protease 1 (MT-SP1) is a membrane anchored protease which is upregulated in epithelial cancers. A dysregulation in MT-SP1/matriptase levels with respect to its cognate inhibitor hepatocyte growth factor activator inhibitor-1 [HAI-1] suggests that it is an increase in proteolytic activity that significantly differentiates malignant from normal tissue. The Craik lab has developed antibodies which bind to and inhibit MT-SP1, but the utility of these probes, and of MT-SP1 activity as a cancer-associated biomarker, has yet to be established. Here we show that these antibodies inhibit the full-length protein on the surface of cancer cells and that they may be functionalized for imaging of this activity <italics>in vivo</italics>. The antibodies were first used to target and inhibit MT-SP1 on human cancer cell lines which express MT-SP1 mRNA. This activity was then targeted <italics>in vivo</italics> using xenograft mouse models of cancer. It was found that the antibodies preferentially localize to tumors which express MT-SP1, suggesting that MT-SP1 activity is a novel biomarker for epithelial cancer and these antibodies provide a non-invasive method for detecting this activity <italics>in vivo</italics>.
Subjects/Keywords: Biology, Molecular; Cancer; Matriptase; Molecular Imaging; Protease; Protease Activity
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APA (6th Edition):
Darragh, M. R. (2010). Targeting the Active Serine Protease MT-SP1 for Tumor Detection <italics>in vivo</italics>. (Thesis). University of California – San Francisco. Retrieved from http://www.escholarship.org/uc/item/69q0228b
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Darragh, Molly Rose. “Targeting the Active Serine Protease MT-SP1 for Tumor Detection <italics>in vivo</italics>.” 2010. Thesis, University of California – San Francisco. Accessed September 17, 2019.
http://www.escholarship.org/uc/item/69q0228b.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Darragh, Molly Rose. “Targeting the Active Serine Protease MT-SP1 for Tumor Detection <italics>in vivo</italics>.” 2010. Web. 17 Sep 2019.
Vancouver:
Darragh MR. Targeting the Active Serine Protease MT-SP1 for Tumor Detection <italics>in vivo</italics>. [Internet] [Thesis]. University of California – San Francisco; 2010. [cited 2019 Sep 17].
Available from: http://www.escholarship.org/uc/item/69q0228b.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Darragh MR. Targeting the Active Serine Protease MT-SP1 for Tumor Detection <italics>in vivo</italics>. [Thesis]. University of California – San Francisco; 2010. Available from: http://www.escholarship.org/uc/item/69q0228b
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Washington University in St. Louis
26.
Song, Liang.
High-Speed Photoacoustic Microscopy In Vivo.
Degree: PhD, Biomedical Engineering, 2010, Washington University in St. Louis
URL: https://openscholarship.wustl.edu/etd/329
► The overarching goal of this research is to develop a novel photoacoustic microscopy: PAM) technology capable of high-speed, high-resolution 3D imaging in vivo. PAM combines…
(more)
▼ The overarching goal of this research is to develop a novel photoacoustic microscopy: PAM) technology capable of high-speed, high-resolution 3D
imaging in vivo. PAM combines the advantages of optical absorption contrast and ultrasonic resolution for deep
imaging beyond the quasi-ballistic regime. Its high sensitivity to optical absorption enables the
imaging of important physiological parameters, such as hemoglobin concentration and oxygen saturation, which closely correlate with angiogenesis and hypermetabolism – two hallmarks of cancer. To translate PAM to the clinic, both high
imaging speed and high spatial resolution are desired. With high spatial resolution, PAM can detect small structural and functional changes early; whereas, high-speed image acquisition helps reduce motion artifacts, patient discomfort, cost, and potentially the risks associated with minimally invasive procedures such as endoscopy and intravascular
imaging. To achieve high
imaging speed, we have constructed a PAM system using a linear ultrasound array and a kHz-repetition-rate tunable laser. The system has achieved a 249-Hz B-scan rate and a 0.5-Hz 3D
imaging rate: over ~6 mm ├ù 10 mm ├ù 3 mm), over 200 times faster than existing mechanical scanning PAM using a single ultrasonic transducer. In addition, high-speed optical-resolution photoacoustic microscopy: OR-PAM) technology has been developed, in which the spatial resolution in one or two dimension(s) is defined by the diffraction-limited optical focus. Using section illumination, the elevational resolution of the system has been improved from ~300 micron to ~28 micron, resulting in a significant improvement in the 3D image quality. Furthermore, multiple optical foci with a microlens array have been used to provide finer than 10-micron lateral resolution – enabling the system to image capillary-level microvessels in vivo – while offering a speed potentially 20 times faster than previously existing single-focus OR-PAM. Finally, potential biomedical applications of the developed technology have been demonstrated through in vivo
imaging of murine sentinel lymph nodes, microcirculation dynamics, and human pulsatile dynamics. In the future, this high-speed PAM technology may be adapted for clinical
imaging of diabetes-induced vascular complications or tumor angiogenesis, or miniaturized for gastrointestinal or intravascular applications.
Advisors/Committee Members: Lihong Wang.
Subjects/Keywords: Medical Imaging and Radiology; Biomedical Engineering; Optics; Cancer, Functional imaging, Microscopy, Molecular imaging, Photoacoustic imaging, Tomography
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APA ·
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MLA ·
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CSE |
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APA (6th Edition):
Song, L. (2010). High-Speed Photoacoustic Microscopy In Vivo. (Doctoral Dissertation). Washington University in St. Louis. Retrieved from https://openscholarship.wustl.edu/etd/329
Chicago Manual of Style (16th Edition):
Song, Liang. “High-Speed Photoacoustic Microscopy In Vivo.” 2010. Doctoral Dissertation, Washington University in St. Louis. Accessed September 17, 2019.
https://openscholarship.wustl.edu/etd/329.
MLA Handbook (7th Edition):
Song, Liang. “High-Speed Photoacoustic Microscopy In Vivo.” 2010. Web. 17 Sep 2019.
Vancouver:
Song L. High-Speed Photoacoustic Microscopy In Vivo. [Internet] [Doctoral dissertation]. Washington University in St. Louis; 2010. [cited 2019 Sep 17].
Available from: https://openscholarship.wustl.edu/etd/329.
Council of Science Editors:
Song L. High-Speed Photoacoustic Microscopy In Vivo. [Doctoral Dissertation]. Washington University in St. Louis; 2010. Available from: https://openscholarship.wustl.edu/etd/329
Washington University in St. Louis
27.
Li, Li.
Photoacoustic Reporter Gene Imaging And Optical Coherence Computed Tomography.
Degree: PhD, Biomedical Engineering, 2010, Washington University in St. Louis
URL: https://openscholarship.wustl.edu/etd/869
► Advances in imaging technologies have always been the major driving forces for the evolution of biomedical research. Compared with other modalities, optical imaging possesses several…
(more)
▼ Advances in
imaging technologies have always been the major driving forces for the evolution of biomedical research. Compared with other modalities, optical
imaging possesses several prominent merits. Because light interacts with tissue at the microscopic level through many distinct physical mechanisms, optical methods allow sensitive exploration of various aspects of the life down to the single-molecule level. From the technical perspective, optical systems utilize safe non-ionizing radiation, could be implemented at relatively low cost, also have the potential to be miniaturized for portable or endoscopic applications. As a result, optical
imaging tools are playing an increasingly important role in both laboratorial research and clinical practice. Among them, photoacoustic
imaging: PAI) and optical coherence tomography: OCT) are the two fastest growing branches. PAI measures the laser-induced acoustic wave, and produces high-resolution images of the optically absorbing features of tissue at multiple length-scales. OCT detects singly backscattered photons, and enables real-time high-resolution in vivo biopsy of tissue up to an optical transport mean-free-path. My doctoral research is focused on developing three novel optical
imaging techniques based on the spirits of PAI and OCT. In the first part of this study, we established a new paradigm to visualize gene expression in vivo based on optical absorption. In the post-genomic era, we are now being challenged to develop novel
molecular imaging methods to identify the functions of genes. PAI can detect specific molecules according to their characteristic absorption spectra, thus is a promising candidate for
molecular imaging of gene expression. The full potential of photoacoustic
molecular imaging still remains to be explored. For the first time, we demonstrated
imaging gene expression by PAI in living mice and rats, using a chromogenic lacZ/X-gal reporter gene system. We demonstrated the expression of the lacZ reporter gene can be detected by PAI as deep as 5 cm inside tissue. In addition, we showcased that PAI could follow gene expression from the microscopic to the macroscopic level. This work represents one of the pioneering efforts to extend photoacoustic methods for
molecular imaging. In the second part of this study, we developed a novel multimodal microscope, called the integrated photoacoustic and optical coherence microscope: iPOM), which combines PAI and OCT in a single
imaging platform. PAI is predominantly sensitive to optical absorption, while OCT exploits optical scattering. By combining their naturally complementary
imaging contrasts, iPOM can provide comprehensive information about biological tissue. We designed and built a reflection-mode prototype of iPOM, which fuses optical-resolution photoacoustic microscopy with spectral-domain optical coherence tomography. The potential applications of iPOM in studying cutaneous and ocular microcirculation, and tissue engineering were demonstrated. Finally, we invented a new optical tomography, named optical…
Advisors/Committee Members: Lihong Wang.
Subjects/Keywords: Biomedical Engineering; Medical Imaging and Radiology; Health Sciences; Gene Expression; Molecular Imaging; Multimodality Imaging; Optical Coherence Tomography; Optical Tomography; Photoacoustic Imaging
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APA ·
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Manager
APA (6th Edition):
Li, L. (2010). Photoacoustic Reporter Gene Imaging And Optical Coherence Computed Tomography. (Doctoral Dissertation). Washington University in St. Louis. Retrieved from https://openscholarship.wustl.edu/etd/869
Chicago Manual of Style (16th Edition):
Li, Li. “Photoacoustic Reporter Gene Imaging And Optical Coherence Computed Tomography.” 2010. Doctoral Dissertation, Washington University in St. Louis. Accessed September 17, 2019.
https://openscholarship.wustl.edu/etd/869.
MLA Handbook (7th Edition):
Li, Li. “Photoacoustic Reporter Gene Imaging And Optical Coherence Computed Tomography.” 2010. Web. 17 Sep 2019.
Vancouver:
Li L. Photoacoustic Reporter Gene Imaging And Optical Coherence Computed Tomography. [Internet] [Doctoral dissertation]. Washington University in St. Louis; 2010. [cited 2019 Sep 17].
Available from: https://openscholarship.wustl.edu/etd/869.
Council of Science Editors:
Li L. Photoacoustic Reporter Gene Imaging And Optical Coherence Computed Tomography. [Doctoral Dissertation]. Washington University in St. Louis; 2010. Available from: https://openscholarship.wustl.edu/etd/869
University of California – Berkeley
28.
Zheng, Bo.
System Hardware and in vivo Cell Tracking in Magnetic Particle Imaging.
Degree: Bioengineering, 2015, University of California – Berkeley
URL: http://www.escholarship.org/uc/item/1pk35215
► Magnetic Particle Imaging (MPI) is an emergent medical imaging technology that directly images the intense magnetization of clinically safe superparamagnetic iron oxide (SPIO) nanoparticles. Because…
(more)
▼ Magnetic Particle Imaging (MPI) is an emergent medical imaging technology that directly images the intense magnetization of clinically safe superparamagnetic iron oxide (SPIO) nanoparticles. Because biological tissues do not produce signals detectable in MPI scanners, MPI images have extremely high image contrast and sensitivity for SPIO tracers, akin to nuclear medicine imaging techniques. The MPI signal is also linearly proportional to SPIO tracer concentrations in the imaging volume, making it a truly quantitative imaging technique. Hence, because of its high image contrast, sensitivity, quantitativeness, and tracer safety, MPI may be useful in applications ranging from coronary angiography to stem cell therapy tracking and is extremely promising for translation to the clinic.The physical basis behind signal generation in MPI is unlike that of any other imaging modality, giving MPI the potential to be one of the most sensitive medical imaging modalities. However, several limitations have prevented existing MPI scanners from reaching the physical limits of detection sensitivity. These limitations include direct feedthrough interference from the MPI transmitter to the receiver, which can obscure the desired SPIO magnetization signal and limit SNR. In Chapter 2 of this dissertation, I aim to determine the sources of interference generation in MPI scanners and to develop engineering solutions to attenuate the feedthrough interference in the detected signal spectrum. My results indicate that feedthrough interference can arise from high-power passive components used to generate the MPI drive fields, as well as from the interaction between the MPI drive field and the magnets used to generate the MPI magnetic field gradient. To remove these interfering signals for improved detection sensitivity, I designed an actively-controlled magnetic interference cancellation system using a Cartesian feedback controller. Data from this active cancellation system has shown the ability to suppress interfering MPI signals by over 55 dB.Another limitation in the sensitivity of existing MPI scanners is the use of non-optimized electronics in the MPI detector chain, which can add substantial noise beyond the noise mechanisms generated in the patient and in the detector coil. In Chapter 3 of this dissertation, I investigate the sources of electronic noise in MPI and describe three methods to reduce noise from the MPI detector preamplifier to below the noise generated by the MPI detector coil. Using these noise-matching techniques, I then describe the design and implementation of a custom transformer-coupled MPI preamplifier. Finally, using a 7 T/m preclinical MPI scanner, I demonstrate that the custom MPI preamplifier can achieve an 11-fold improvement in the signal-noise ratio (SNR) of MPI scanners over a commercially available low-noise preamplifier.Building upon these improvements in the sensitivity and SNR of our preclinical MPI scanners, I then perform the first two in vivo experiments to track implanted stem cell therapies in rodent…
Subjects/Keywords: Medical imaging; Biomedical engineering; Electrical engineering; Cell Tracking; Magnetic Particle Imaging; Medical Imaging; Molecular Imaging; Noise matching
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APA (6th Edition):
Zheng, B. (2015). System Hardware and in vivo Cell Tracking in Magnetic Particle Imaging. (Thesis). University of California – Berkeley. Retrieved from http://www.escholarship.org/uc/item/1pk35215
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Zheng, Bo. “System Hardware and in vivo Cell Tracking in Magnetic Particle Imaging.” 2015. Thesis, University of California – Berkeley. Accessed September 17, 2019.
http://www.escholarship.org/uc/item/1pk35215.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Zheng, Bo. “System Hardware and in vivo Cell Tracking in Magnetic Particle Imaging.” 2015. Web. 17 Sep 2019.
Vancouver:
Zheng B. System Hardware and in vivo Cell Tracking in Magnetic Particle Imaging. [Internet] [Thesis]. University of California – Berkeley; 2015. [cited 2019 Sep 17].
Available from: http://www.escholarship.org/uc/item/1pk35215.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Zheng B. System Hardware and in vivo Cell Tracking in Magnetic Particle Imaging. [Thesis]. University of California – Berkeley; 2015. Available from: http://www.escholarship.org/uc/item/1pk35215
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
University of California – San Diego
29.
Chen, Chien-Ju.
Lipid-polymer hybrid nanoparticles: A photoacoustic imaging tool for ovarian cancer diagnosis.
Degree: Bioengineering, 2017, University of California – San Diego
URL: http://www.escholarship.org/uc/item/8735507k
► The survival rate of patients with ovarian cancer is over 90% in Stages I and II but detection strategies based on CA125 testing are ineffective.…
(more)
▼ The survival rate of patients with ovarian cancer is over 90% in Stages I and II but detection strategies based on CA125 testing are ineffective. Hence, this screening approach not only fails to decrease ovarian cancer mortality but also causes significant harm including major surgery in cancer free women. Fortunately, the metrics of ultrasound-based techniques are encouraging—a recent large scale trial of over 200,000 women indicated that the sensitivity and specificity were 89.4% and 99.8%, respectively, when combined with CA125 tests. However, the positive predictive value of ovarian cancer was only 43.3%. Therefore, we hypothesize that using photoacoustic ultrasoundcombined with lipid-polymer hybrid nanoparticles can achieve advances in specificity and sensitivity that are need in population-wide studies. In our study, we used poly-lacticco- glutamic acid (PLGA), lecithin, and 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[folate(polyethylene glycol)]-5000 (DSPE-PEG-folate) to fabricate hybrid nanoparticles. The photoacoustic imaging agents DiR or IR-1061 were encapsulated in PLGA hydrophobic core. The folate-functionalized nanoparticles can actively target HeLa cells which overexpress folate receptors on their cell membrane. The main hypothesis is that the folate-functionalized nanoparticles could produce highercontrast between ovarian cancer tissues and normal tissues than nanoparticles without folate modification. Also, we proved our IR-1061-loaded nanoparticles had a pH-responsive release profile. We can even use photoacoustic imaging to track the releasingIR-1061 in PBS at different pH values. In the future, we attempt to conjugate IR-1061 molecules with anti-cancer drugs to endow our nanoparticles theranostic capability. Our long-term goal is to achieve in vivo photoacoustic imaging and theranostic treatment of ovarian cancer patients.
Subjects/Keywords: Biomedical engineering; bioimaging; IR-1061; molecular imaging; nanoparticles; ovarian cancer diagnosis; photoacoustic imaging
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APA ·
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MLA ·
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Export
to Zotero / EndNote / Reference
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APA (6th Edition):
Chen, C. (2017). Lipid-polymer hybrid nanoparticles: A photoacoustic imaging tool for ovarian cancer diagnosis. (Thesis). University of California – San Diego. Retrieved from http://www.escholarship.org/uc/item/8735507k
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Chen, Chien-Ju. “Lipid-polymer hybrid nanoparticles: A photoacoustic imaging tool for ovarian cancer diagnosis.” 2017. Thesis, University of California – San Diego. Accessed September 17, 2019.
http://www.escholarship.org/uc/item/8735507k.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Chen, Chien-Ju. “Lipid-polymer hybrid nanoparticles: A photoacoustic imaging tool for ovarian cancer diagnosis.” 2017. Web. 17 Sep 2019.
Vancouver:
Chen C. Lipid-polymer hybrid nanoparticles: A photoacoustic imaging tool for ovarian cancer diagnosis. [Internet] [Thesis]. University of California – San Diego; 2017. [cited 2019 Sep 17].
Available from: http://www.escholarship.org/uc/item/8735507k.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Chen C. Lipid-polymer hybrid nanoparticles: A photoacoustic imaging tool for ovarian cancer diagnosis. [Thesis]. University of California – San Diego; 2017. Available from: http://www.escholarship.org/uc/item/8735507k
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Ryerson University
30.
Zalev, Jason.
Detection and monitoring for cancer and abnormal vasculature by photoacoustic signal characterization of structural morphology.
Degree: 2010, Ryerson University
URL: https://digital.library.ryerson.ca/islandora/object/RULA%3A1155
► Photoacoustic systems can produce high-resolution, high-contracts images of vascular structures. To reconstruct images at very high-resolution, signals must be collected from many transducer locations, which…
(more)
▼ Photoacoustic systems can produce high-resolution, high-contracts images of vascular structures. To reconstruct images at very high-resolution, signals must be collected from many transducer locations, which can be time consuming due to limitations in transducer array technology, In this thesis a method is presented to discriminate between normal and abnormal tissue based on the structural morphology of vasculature and permits data to be acquired quickly. To demonstrate that the approach may be useful for cancer detection, a special simulator that produces photoacoustic signal from 3D models of vascular tissue is developed. Validation of the simulator is performed against a derived exact equation for finite-length cylindrical photoacoustic sources and through FEM models. Results show that is possible to differentiate tissue classed even when it is not possible to resolve individual blood vessels. Performance of the algorithm remains strong as the number of transducer locations decreases and in the presence of noise.
Advisors/Committee Members: Kolios, Michael C. (Thesis advisor), Ryerson University (Degree grantor).
Subjects/Keywords: Acoustic imaging; Imaging systems in medicine; Cancer – Molecular aspects; Cellular signal transduction
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APA ·
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APA (6th Edition):
Zalev, J. (2010). Detection and monitoring for cancer and abnormal vasculature by photoacoustic signal characterization of structural morphology. (Thesis). Ryerson University. Retrieved from https://digital.library.ryerson.ca/islandora/object/RULA%3A1155
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Zalev, Jason. “Detection and monitoring for cancer and abnormal vasculature by photoacoustic signal characterization of structural morphology.” 2010. Thesis, Ryerson University. Accessed September 17, 2019.
https://digital.library.ryerson.ca/islandora/object/RULA%3A1155.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Zalev, Jason. “Detection and monitoring for cancer and abnormal vasculature by photoacoustic signal characterization of structural morphology.” 2010. Web. 17 Sep 2019.
Vancouver:
Zalev J. Detection and monitoring for cancer and abnormal vasculature by photoacoustic signal characterization of structural morphology. [Internet] [Thesis]. Ryerson University; 2010. [cited 2019 Sep 17].
Available from: https://digital.library.ryerson.ca/islandora/object/RULA%3A1155.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Zalev J. Detection and monitoring for cancer and abnormal vasculature by photoacoustic signal characterization of structural morphology. [Thesis]. Ryerson University; 2010. Available from: https://digital.library.ryerson.ca/islandora/object/RULA%3A1155
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
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Open Access Theses and Dissertations