subject:(Molecular beacon)

University of Alberta

1. Shire, Zahra J. Fluorescent Hairpin Probes for the Detection of Chemically-Induced DNA Damage.

Degree: MS, Department of Chemistry, 2012, University of Alberta

URL: https://era.library.ualberta.ca/files/h702q775d

► In this thesis, we develop a sensitive, robust, accurate method to detect chemically-induced DNA damage by three therapeutic agents, cisplatin, psoralen and busulfan. The method… (more)

Subjects/Keywords: Hairpin probe; DNA damage; Molecular beacon

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APA (6^th Edition):

Shire, Z. J. (2012). Fluorescent Hairpin Probes for the Detection of Chemically-Induced DNA Damage. (Masters Thesis). University of Alberta. Retrieved from https://era.library.ualberta.ca/files/h702q775d

Chicago Manual of Style (16^th Edition):

Shire, Zahra J. “Fluorescent Hairpin Probes for the Detection of Chemically-Induced DNA Damage.” 2012. Masters Thesis, University of Alberta. Accessed January 18, 2021. https://era.library.ualberta.ca/files/h702q775d.

MLA Handbook (7^th Edition):

Shire, Zahra J. “Fluorescent Hairpin Probes for the Detection of Chemically-Induced DNA Damage.” 2012. Web. 18 Jan 2021.

Vancouver:

Shire ZJ. Fluorescent Hairpin Probes for the Detection of Chemically-Induced DNA Damage. [Internet] [Masters thesis]. University of Alberta; 2012. [cited 2021 Jan 18]. Available from: https://era.library.ualberta.ca/files/h702q775d.

Council of Science Editors:

Shire ZJ. Fluorescent Hairpin Probes for the Detection of Chemically-Induced DNA Damage. [Masters Thesis]. University of Alberta; 2012. Available from: https://era.library.ualberta.ca/files/h702q775d

Vanderbilt University

2. Jackson, Stephen Randall. DNA-functionalized Gold Nanoparticles for Enhanced Molecular Sensing.

Degree: PhD, Chemistry, 2014, Vanderbilt University

URL: http://hdl.handle.net/1803/12860

► Since the advent of molecular beacon technology, much attention has been directed toward understanding RNA expression at a single-cell level. Our laboratory has designed an… (more)

▼ Since the advent of molecular beacon technology, much attention has been directed toward understanding RNA expression at a single-cell level. Our laboratory has designed an approach that harnesses the advantages of both molecular beacon and gold nanoparticle technology, termed hairpin DNA-functionalized gold nanoparticles (hAuNPs). hAuNPs improve upon technologies for studying RNA trafficking by their efficient internalization within live cells without transfection reagents, improved resistance to DNAse degradation, low cytotoxicity, and high specificity and sensitivity toward the target mRNA sequence. This dissertation highlights efforts to optimize the design of hAuNPs as well as new applications of these novel mRNA imaging probes. The optimization of the existing hAuNP construct resulted in a four-fold increase in signal-to-noise ratio. hAuNPs were used to image multiple biomarkers in three different types of living cancer cells. The fluorescence intensities for the probes in cells were measured by flow cytometry and confocal microscopy and showed good agreement with reported values for their target mRNA expression, thus confirming the ability to measure relative expression levels of mRNAs in living cells using hAuNPs. Specific imaging of RNA biomarkers was achieved in retinal tissue using in vivo retinal fluorescence imaging of laser-induced choroidal neovascularization in animal models. The use of gold nanorods (AuNRs) as the nanoparticle construct of hAuNPs was also investigated. Hairpin DNA functionalization was achieved on AuNRs after an overgrowth step in which a thin layer of gold atoms were reduced on a preformed AuNR, masking the trace silver ions that are thought to complicate other functionalization strategies. Finally, scanning transmission electron microscopy-energy dispersive X-ray spectroscopy (STEM-EDS) mapping of AuNRs reveal new information on the structural and elemental characterization of AuNRs specifically the location of silver deposits on the surface of the rods, providing new insight into the mechanism by which AuNRs grow. Advisors/Committee Members: Sandra J. Rosenthal (committee member), Frederick R. Haselton (committee member), Eva M. Harth (committee member), David W. Wright (Committee Chair).

Subjects/Keywords: gold nanoparticle; mRNA; molecular beacon; nanorod; hAuNP

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APA (6^th Edition):

Jackson, S. R. (2014). DNA-functionalized Gold Nanoparticles for Enhanced Molecular Sensing. (Doctoral Dissertation). Vanderbilt University. Retrieved from http://hdl.handle.net/1803/12860

Chicago Manual of Style (16^th Edition):

Jackson, Stephen Randall. “DNA-functionalized Gold Nanoparticles for Enhanced Molecular Sensing.” 2014. Doctoral Dissertation, Vanderbilt University. Accessed January 18, 2021. http://hdl.handle.net/1803/12860.

MLA Handbook (7^th Edition):

Jackson, Stephen Randall. “DNA-functionalized Gold Nanoparticles for Enhanced Molecular Sensing.” 2014. Web. 18 Jan 2021.

Vancouver:

Jackson SR. DNA-functionalized Gold Nanoparticles for Enhanced Molecular Sensing. [Internet] [Doctoral dissertation]. Vanderbilt University; 2014. [cited 2021 Jan 18]. Available from: http://hdl.handle.net/1803/12860.

Council of Science Editors:

Jackson SR. DNA-functionalized Gold Nanoparticles for Enhanced Molecular Sensing. [Doctoral Dissertation]. Vanderbilt University; 2014. Available from: http://hdl.handle.net/1803/12860

University of Toronto

3. Luby, Benjamin Mark. Amplified Detection of microRNA with a Novel Fluorescent Oligonucleotide Probe: microRNA:Argonaute Cleavage (miRACle) Beacons.

Degree: PhD, 2020, University of Toronto

URL: http://hdl.handle.net/1807/101081

► This thesis concerns the development and exploration of a new class of fluorogenic microRNA sensors which exploit cellular processes to enable new capabilities. These sensors… (more)

▼ This thesis concerns the development and exploration of a new class of fluorogenic microRNA sensors which exploit cellular processes to enable new capabilities. These sensors consist of a fluorophore and matching quencher, tethered to opposite ends of an oligonucleotide strand. Previously, miRNA sensors were simple adaptations from DNA-targeting sensors and relied on simple hybridization to achieve their specificity. MicroRNAs, however, are genetic sequences that are only one component of the multi-protein complex called the RNA-induced Silencing Complex (RISC), a major regulatory nexus for gene expression present in eukaryotic cells. This new class of sensor binds to a specific microRNA within RISC and undergoes enzymatic processing through the endonuclease Argonaute-2 to permanently activate fluorescence. This microRNA:Argonaute Cleavage (miRACle) beacon was investigated in a recombinant system and validated in solution with immunoprecipitated RISC. Fluorescence upon miRACle beacon activation increased 13-fold above hybridization and showed single nucleotide specificity. After validating the concept, the project split into two avenues of research. The first avenue was evaluating if the system could discern between frame-shifted miRNAs, termed isomiRs. To investigate this, common nuclease resistance modifications were shifted along the oligonucleotide strand to assess their impact on assay performance. Modifications within the catalytic site abrogated activity, demonstrating that miRACle beacons may detect frame shifted isomiRs correctly. The second avenue for miRACle beacons focused on bringing the assay into cells and resisting the processes that give false positive signals. Established strategies to prevent non-specific nuclear accumulation or nuclease degradation were screened to determine compatible modifications to the assay. A porphyrin dye, pyropheophorbide-a, could exclude the beacons from the nucleus in vitro, however no tested strategy definitively showed miRNA specific activation and stability to non-specific nucleases in vitro Advisors/Committee Members: Zheng, Gang, Biomedical Engineering.

Subjects/Keywords: microRNA; Molecular Beacon; Probe; Sensor; 0541

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APA (6^th Edition):

Luby, B. M. (2020). Amplified Detection of microRNA with a Novel Fluorescent Oligonucleotide Probe: microRNA:Argonaute Cleavage (miRACle) Beacons. (Doctoral Dissertation). University of Toronto. Retrieved from http://hdl.handle.net/1807/101081

Chicago Manual of Style (16^th Edition):

Luby, Benjamin Mark. “Amplified Detection of microRNA with a Novel Fluorescent Oligonucleotide Probe: microRNA:Argonaute Cleavage (miRACle) Beacons.” 2020. Doctoral Dissertation, University of Toronto. Accessed January 18, 2021. http://hdl.handle.net/1807/101081.

MLA Handbook (7^th Edition):

Luby, Benjamin Mark. “Amplified Detection of microRNA with a Novel Fluorescent Oligonucleotide Probe: microRNA:Argonaute Cleavage (miRACle) Beacons.” 2020. Web. 18 Jan 2021.

Vancouver:

Luby BM. Amplified Detection of microRNA with a Novel Fluorescent Oligonucleotide Probe: microRNA:Argonaute Cleavage (miRACle) Beacons. [Internet] [Doctoral dissertation]. University of Toronto; 2020. [cited 2021 Jan 18]. Available from: http://hdl.handle.net/1807/101081.

Council of Science Editors:

Luby BM. Amplified Detection of microRNA with a Novel Fluorescent Oligonucleotide Probe: microRNA:Argonaute Cleavage (miRACle) Beacons. [Doctoral Dissertation]. University of Toronto; 2020. Available from: http://hdl.handle.net/1807/101081

Utah State University

4. Davis, Michael L. Excimer-Monomer Switching Molecular Beacon: The Study on Synthetic Cryptosporidium DNA Detection, Thermodynamics, and Magnesium Effects.

Degree: MS, Biological and Irrigation Engineering, 2014, Utah State University

URL: https://digitalcommons.usu.edu/etd/2191

► Cryptosporidium parvum is a deadly waterborne protozoan parasite that invades the gastrointestinal tract of humans and causes severe to life-threatening gastro enteric disease. Due… (more)

Subjects/Keywords: Molecular; Beacon; Cryptosporidium; Thermodynamics; Biological Engineering

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APA (6^th Edition):

Davis, M. L. (2014). Excimer-Monomer Switching Molecular Beacon: The Study on Synthetic Cryptosporidium DNA Detection, Thermodynamics, and Magnesium Effects. (Masters Thesis). Utah State University. Retrieved from https://digitalcommons.usu.edu/etd/2191

Chicago Manual of Style (16^th Edition):

Davis, Michael L. “Excimer-Monomer Switching Molecular Beacon: The Study on Synthetic Cryptosporidium DNA Detection, Thermodynamics, and Magnesium Effects.” 2014. Masters Thesis, Utah State University. Accessed January 18, 2021. https://digitalcommons.usu.edu/etd/2191.

MLA Handbook (7^th Edition):

Davis, Michael L. “Excimer-Monomer Switching Molecular Beacon: The Study on Synthetic Cryptosporidium DNA Detection, Thermodynamics, and Magnesium Effects.” 2014. Web. 18 Jan 2021.

Vancouver:

Davis ML. Excimer-Monomer Switching Molecular Beacon: The Study on Synthetic Cryptosporidium DNA Detection, Thermodynamics, and Magnesium Effects. [Internet] [Masters thesis]. Utah State University; 2014. [cited 2021 Jan 18]. Available from: https://digitalcommons.usu.edu/etd/2191.

Council of Science Editors:

Davis ML. Excimer-Monomer Switching Molecular Beacon: The Study on Synthetic Cryptosporidium DNA Detection, Thermodynamics, and Magnesium Effects. [Masters Thesis]. Utah State University; 2014. Available from: https://digitalcommons.usu.edu/etd/2191

Université de Lorraine

5. Verhille, Marc. Conception, élaboration et caractérisation photophysiques et biochimiques de molécules photoactivables pour la thérapie photodynamique : Selective antitumor effect in PhotoDynamic Therapy mediated by photo-activable molecules, activated by endopeptidases.

Degree: Docteur es, Génie des Procédés et des Produits, 2012, Université de Lorraine

URL: http://www.theses.fr/2012LORR0194

►

L'angiogenèse est une étape clef dans le processus de progression tumorale. Elle est caractérisée par une surexpresssion d'un grand nombre de métalloprotéinases matricielles (MMP). Parmis… (more)

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L'angiogenèse est une étape clef dans le processus de progression tumorale. Elle est caractérisée par une surexpresssion d'un grand nombre de métalloprotéinases matricielles (MMP). Parmis ces MMP, les gélatinases (MMP-2 et MMP-9) sont connues pour jouer un rôle important dans l'angiogenèse tumorale et la croissance de nombreux cancers. Les "Photodynamic Molecular Beacon" (PMB) sont des constructions moléculaires qui peuvent être utilisées dans le traitement de cancers en associant un photosensibilisateur (PS) de type chlorine et un inhibiteur d'états excités, aussi appelé "Quencher", liés par un peptide substrat des gélatinases afin d'inhiber la toxicité du PS dans les cellules non ciblées, et de restaurer sa toxicité uniquement à proximité des gélatinases. Nous avons donc cherché à déterminer le couple PS/quencher permettant la meilleure inhibition de la production d'oxygène singulet, principale source de la toxicité du PS, puis avons synthétisé une famille de PMB ciblant les gélatinases. Différents peptides et bras espaceurs ont été utilisés pour évaluer l'influence de la distance entre le PS et le quencher sur les propriétés photophysiques et l'activation enzymatique du PMB

Angiogenesis is a key step in the tumoral progression process. It is characterized by an over-expression of a number of matrix metalloproteinases (MMP). Among these MMPs, gelatinases (MMP-2 and MMP-9) are known to play a critical role in tumor angiogenesis and the growth of many cancers. Photodynamic Molecular Beacons (PMB) can be designed for cancer treatment by associating a chlorin-like photosensitizer and a black hole quencher linked by a gelatinase substrate peptide with the aim of silencing photosensitizer toxicity in non-targeted cells and restore its toxicity only in surrounding gelatinases. We investigated the PS/quencher pair allowing the best singlet oxygen production inhibition, and then we synthesized a novel family of PMB triggering gélatinases MMP-2 and MMP-9. Different lengths of peptide and spacers were used in order to determinate the influence of the distance between PS and quencher on the PMB photophysical properties and enzymatic activation.

Advisors/Committee Members: Frochot, Céline (thesis director).

Subjects/Keywords: Photodynamic Molecular Beacon (PMB); Métalloprotéinases Matricielles (MMP); Fret; Ciblage; Photodynamic Molecular Beacon (PMB); Matrix Metalloproteinases (MMP); Fret; Targetting; 616.994 0631; 572.76

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APA (6^th Edition):

Verhille, M. (2012). Conception, élaboration et caractérisation photophysiques et biochimiques de molécules photoactivables pour la thérapie photodynamique : Selective antitumor effect in PhotoDynamic Therapy mediated by photo-activable molecules, activated by endopeptidases. (Doctoral Dissertation). Université de Lorraine. Retrieved from http://www.theses.fr/2012LORR0194

Chicago Manual of Style (16^th Edition):

Verhille, Marc. “Conception, élaboration et caractérisation photophysiques et biochimiques de molécules photoactivables pour la thérapie photodynamique : Selective antitumor effect in PhotoDynamic Therapy mediated by photo-activable molecules, activated by endopeptidases.” 2012. Doctoral Dissertation, Université de Lorraine. Accessed January 18, 2021. http://www.theses.fr/2012LORR0194.

MLA Handbook (7^th Edition):

Verhille, Marc. “Conception, élaboration et caractérisation photophysiques et biochimiques de molécules photoactivables pour la thérapie photodynamique : Selective antitumor effect in PhotoDynamic Therapy mediated by photo-activable molecules, activated by endopeptidases.” 2012. Web. 18 Jan 2021.

Vancouver:

Verhille M. Conception, élaboration et caractérisation photophysiques et biochimiques de molécules photoactivables pour la thérapie photodynamique : Selective antitumor effect in PhotoDynamic Therapy mediated by photo-activable molecules, activated by endopeptidases. [Internet] [Doctoral dissertation]. Université de Lorraine; 2012. [cited 2021 Jan 18]. Available from: http://www.theses.fr/2012LORR0194.

Council of Science Editors:

Verhille M. Conception, élaboration et caractérisation photophysiques et biochimiques de molécules photoactivables pour la thérapie photodynamique : Selective antitumor effect in PhotoDynamic Therapy mediated by photo-activable molecules, activated by endopeptidases. [Doctoral Dissertation]. Université de Lorraine; 2012. Available from: http://www.theses.fr/2012LORR0194

NSYSU

6. Nieh, Chih-chun. (1) Molecular beacon for fluorescent assay of enzyme activity and its inhibition; (2) A new strategy for preparing gold nanoclusters.

Degree: Master, Chemistry, 2014, NSYSU

URL: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0614114-213623

► The thesis consists of two parts. In the first part, we present that a thymine (T)-based molecular beacon (MB) is used for probing S-adenosylhomocysteine hydrolase… (more)

▼ The thesis consists of two parts. In the first part, we present that a thymine (T)-based molecular beacon (MB) is used for probing S-adenosylhomocysteine hydrolase (SAHH)-catalyzed hydrolysis of S- adenosylhomocysteine (SAH), and for following the inhibition of SAHH. The designed MB (T8-MB-T8) contains a 15-mer loop and a stem consisting of a pair of 8-mer T bases, a fluorophore unit at the 5'-end, and a quencher unit at the 3'-end. When Hg2+ induced T-T mismatches to form stable T-Hg2+-T coordination in the MB stem, a change in the conformation of T8-MB-T8 places fluorophore and quencher in proximity to each other and causes in the collisional quenching of fluorescence between them. SAHH catalyzed the hydrolysis of SAH to produce homocysteine. The generated homocysteine enables Hg2+ to remove from a hairpin-shaped T8-MB-T8 through the formation of strong Hg2+-S bond, leading to the restoration of its fluorescence. The T8-MB-T8.Hg2+ probe showed a limit of detection for SAHH of 4 units L-1 (ï½0.24 nM) and is reusable for detecting the SAHH/SAH system. Because adenosine is effective to inhibit SAHH activity, the T8-MB-T8.Hg2+ probe combined the SAHH/SAH system was used for sensitive and selective detection of adenosine in urine and serum without the interferences of other adenosine analogues. In the second part, we developed a new strategy for the production of gold nanoclusters based on the fact that fenton reaction is capable of inducing etching of gold nanoparticles. This top down-based synthesis of gold nanoclusters follows two steps: (1) in the presence of glutathione, reduction of the Au3+ precursor solution with NaBH4 resulted in the formation of gold nanoparticles and (2) the formed gold nanoparticles was etched to gold nanoclusters via Fenton reaction. To obtain atomically precise gold nanoclusters and enhanced their quantum yields, different types of proteins were used for size focusing of the formed gold nanoclusters. The maximal emission of trypsin-, bovine serum albumin-, lysozyme type VI-, and glucose oxidase-stabilized gold nanoclusters appears at 415, 463, 460, and 535 nm, suggesting the presence of Au5, Au8, Au8, and Au13 clusters, respectively. (Matrix-assisted laser desorption/ionization)-(time-of-flight) mass spectrometry and slab-gel native electrophoresis were utilized to demonstrate the formation of gold nanoclusters. Because glutathione can efficiently etch trypsin-stabilized Au5, bovine serum albumin-stabilized Au8, and lysozyme type VI-stabilized Au8, these clusters were well suited to probe glutathione. Glucose oxidase-functionalized Au13 clusters were utilized to detect glucose via the combination of glucose-glucose oxidase catalyzed reaction and H2O2-induced quenching of gold nanoclusters. In addition to the application of gold nanoclusters as optical sensors, we disclose that gold nanoclusters as catalysis for conversion of 4-nitrophenol to 4-aminophenol were more efficient than glutathione- and citrate-capped gold nanoparticles. Advisors/Committee Members: Wei-Lung Tseng (committee member), Che-Hsin Lin (chair), Chun-hu Chen (chair).

Subjects/Keywords: fluorescence; glutathione; S-adenosylhomocysteine hydrolase; gold nanocluster; adenosine; Molecular beacon

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APA (6^th Edition):

Nieh, C. (2014). (1) Molecular beacon for fluorescent assay of enzyme activity and its inhibition; (2) A new strategy for preparing gold nanoclusters. (Thesis). NSYSU. Retrieved from http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0614114-213623

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Nieh, Chih-chun. “(1) Molecular beacon for fluorescent assay of enzyme activity and its inhibition; (2) A new strategy for preparing gold nanoclusters.” 2014. Thesis, NSYSU. Accessed January 18, 2021. http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0614114-213623.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Nieh, Chih-chun. “(1) Molecular beacon for fluorescent assay of enzyme activity and its inhibition; (2) A new strategy for preparing gold nanoclusters.” 2014. Web. 18 Jan 2021.

Vancouver:

Nieh C. (1) Molecular beacon for fluorescent assay of enzyme activity and its inhibition; (2) A new strategy for preparing gold nanoclusters. [Internet] [Thesis]. NSYSU; 2014. [cited 2021 Jan 18]. Available from: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0614114-213623.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Nieh C. (1) Molecular beacon for fluorescent assay of enzyme activity and its inhibition; (2) A new strategy for preparing gold nanoclusters. [Thesis]. NSYSU; 2014. Available from: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0614114-213623

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Rice University

7. Huang, Kewei. Long-lived Luminescent Metal Complexes for Molecules Sensing and Nanotube Dispersion.

Degree: PhD, Natural Sciences, 2014, Rice University

URL: http://hdl.handle.net/1911/87811

► Phosphorescent heavy-metal complexes are one class of excellent photoluminescent materials. The heavy metal-induced spin–orbit coupling leads to singlet–triplet state mixing, thus decreases the “spin-forbidden” component… (more)

Subjects/Keywords: Iridium; Ruthenium; Molecular Beacon; Cysteine; Histidine; Nanotube, Dispersion.

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APA (6^th Edition):

Huang, K. (2014). Long-lived Luminescent Metal Complexes for Molecules Sensing and Nanotube Dispersion. (Doctoral Dissertation). Rice University. Retrieved from http://hdl.handle.net/1911/87811

Chicago Manual of Style (16^th Edition):

Huang, Kewei. “Long-lived Luminescent Metal Complexes for Molecules Sensing and Nanotube Dispersion.” 2014. Doctoral Dissertation, Rice University. Accessed January 18, 2021. http://hdl.handle.net/1911/87811.

MLA Handbook (7^th Edition):

Huang, Kewei. “Long-lived Luminescent Metal Complexes for Molecules Sensing and Nanotube Dispersion.” 2014. Web. 18 Jan 2021.

Vancouver:

Huang K. Long-lived Luminescent Metal Complexes for Molecules Sensing and Nanotube Dispersion. [Internet] [Doctoral dissertation]. Rice University; 2014. [cited 2021 Jan 18]. Available from: http://hdl.handle.net/1911/87811.

Council of Science Editors:

Huang K. Long-lived Luminescent Metal Complexes for Molecules Sensing and Nanotube Dispersion. [Doctoral Dissertation]. Rice University; 2014. Available from: http://hdl.handle.net/1911/87811

East Tennessee State University

8. McGoldrick, Christopher Allen. Novel Ester Substrates for the Detection and Treatment of Prostate Cancer.

Degree: PhD, Biomedical Sciences, 2013, East Tennessee State University

URL: https://dc.etsu.edu/etd/2308

► Cancer cell esterases are often overexpressed and some have chiral specificities different from those of corresponding normal cells. Carboxylesterases in particular are known to… (more)

▼ Cancer cell esterases are often overexpressed and some have chiral specificities different from those of corresponding normal cells. Carboxylesterases in particular are known to be overexpressed in several cancers. Additionally, cancer cells often exhibit high levels of intrinsic oxidative stress that is required for survival and an aggressive phenotype. We hypothesized that these 2 characteristics of cancer cells could be exploited to aid in the detection and treatment of prostate cancer. We have developed a fluorogenic ester probe that is activated by carboxylesterase to help distinguish tumorigenic cells from nontumorigenic prostate cells. Ester prodrugs have the same activation mechanism and have been thought to be a promising approach in cancer therapy. Prodrugs are inactive drugs that can be selectively activated by a specific enzyme. We have developed a chiral ester prodrug strategy using native polyacrylamide gel electrophoresis (n-PAGE) and proteomic methods to compare and identify the esterase profiles of several tumorigenic and nontumorigenic prostate cell lines. Our results showed that cell lysates from LNCaP, DU 145, and PC3 prostate cancer cell lines exhibit differential esterase activity compared with non-tumorigenic RWPE-1 prostate cell lysates when incubated with α- naphthyl acetate or α-naphthyl N-acetyl-alaninate ester substrates and a diazonium salt. We have identified oxidized protein hydrolase (OPH), a serine esterase/protease that catalyzes the removal of N-acylated residues from proteins, to be differentially expressed between some tumorigenic and nontumorigenic prostate cell lines. OPH was found to have high hydrolytic activity towards the S-isomer of α-naphthyl N-acetylalaninate (S-ANAA) chiral ester. LNCaP lysates incubated with N-acetyl-alanyl-p-nitroanilide, a known OPH substrate, had twofold higher OPH activity compared with RWPE-1 lysates. We have also developed and tested novel glutathione depleting prodrugs modeled after S-ANAA that increase oxidative stress and induced apoptosis in tumorigenic prostate cells with little effect on nontumorigenic RWPE-1 cells. These results suggest that ester molecular beacon probes and ester prodrugs may be effective in identifying and treating prostate cancer tumors that overexpress esterases with little effect on normal prostate cells.

Subjects/Keywords: oxidized protein hydrolase; prodrug; carboxylesterase; prostate cancer; molecular beacon; Biochemistry

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APA (6^th Edition):

McGoldrick, C. A. (2013). Novel Ester Substrates for the Detection and Treatment of Prostate Cancer. (Doctoral Dissertation). East Tennessee State University. Retrieved from https://dc.etsu.edu/etd/2308

Chicago Manual of Style (16^th Edition):

McGoldrick, Christopher Allen. “Novel Ester Substrates for the Detection and Treatment of Prostate Cancer.” 2013. Doctoral Dissertation, East Tennessee State University. Accessed January 18, 2021. https://dc.etsu.edu/etd/2308.

MLA Handbook (7^th Edition):

McGoldrick, Christopher Allen. “Novel Ester Substrates for the Detection and Treatment of Prostate Cancer.” 2013. Web. 18 Jan 2021.

Vancouver:

McGoldrick CA. Novel Ester Substrates for the Detection and Treatment of Prostate Cancer. [Internet] [Doctoral dissertation]. East Tennessee State University; 2013. [cited 2021 Jan 18]. Available from: https://dc.etsu.edu/etd/2308.

Council of Science Editors:

McGoldrick CA. Novel Ester Substrates for the Detection and Treatment of Prostate Cancer. [Doctoral Dissertation]. East Tennessee State University; 2013. Available from: https://dc.etsu.edu/etd/2308

University of Illinois – Urbana-Champaign

9. Tan, Yafang. Highly sensitive biosensors using photonic crystal enhanced fluorescence.

Degree: PhD, 1200, 2015, University of Illinois – Urbana-Champaign

URL: http://hdl.handle.net/2142/73064

► Due to the importance of fluorescence assays in clinics, life science and genomic research, many nanostructures have been proposed to enhance the weak fluorescence signals… (more)

▼ Due to the importance of fluorescence assays in clinics, life science and genomic research, many nanostructures have been proposed to enhance the weak fluorescence signals from low abundance analytes. Among these structures, photonic crystal (PC) has attracted particular attentions because of its capability to produce high electric fields without quenching fluorescence emission. Properly designed PC surface can efficiently couple laser illumination to fluorescent dye molecules (enhanced excitation) and meanwhile effectively collects fluorescence emission (enhanced extraction). In this dissertation, photonic crystal enhanced fluorescence (PCEF) was used for disease biomarker dissertation. We applied PCEF to multiplexed microspot immunoassays for detection of allergen-specific antibodies. The PCEF system was able to detect low concentrations of Fel d1 specific IgE ~0.02 kU/L, which is 5-fold to 17-fold more sensitive than the commercially available FDA-approved analyzers. We also aimed to improve the current platform by incorporating a PC surface with a microfluidic chip in order to enable a rapid and automatic biomarker analysis at microliter sample consumption. We fabricated a plastic microfluidic device for holding the PC using a low-cost and flexible process and developed a leak-free automation system to introduce the correct sequence of fluids through the microfluidic device. The PCEF platform was successfully automated to enable more rapid, high sensitivity detection and a simplified output that will be readily usable by a clinician. Besides microarray immunoassays, the PCEF platform was also adapted to a molecular beacon assay for miRNA detection, where the PC surface was incorporated with a submicron-height fluid channel. The use of the PCEF platform results in a reduction of the detection limits and an increase in the signal-to-noise ratio (SNR) with a small, inexpensive detection instrument. Advisors/Committee Members: Cunningham, Brian T. (advisor), Cunningham, Brian T. (Committee Chair), Gong, Songbin (committee member), Liu, Gang (committee member), Vodkin, Lila O. (committee member).

Subjects/Keywords: Photonic Crystal Enhanced Fluorescence (PCEF); Immunoassays; Molecular beacon assays

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APA (6^th Edition):

Tan, Y. (2015). Highly sensitive biosensors using photonic crystal enhanced fluorescence. (Doctoral Dissertation). University of Illinois – Urbana-Champaign. Retrieved from http://hdl.handle.net/2142/73064

Chicago Manual of Style (16^th Edition):

Tan, Yafang. “Highly sensitive biosensors using photonic crystal enhanced fluorescence.” 2015. Doctoral Dissertation, University of Illinois – Urbana-Champaign. Accessed January 18, 2021. http://hdl.handle.net/2142/73064.

MLA Handbook (7^th Edition):

Tan, Yafang. “Highly sensitive biosensors using photonic crystal enhanced fluorescence.” 2015. Web. 18 Jan 2021.

Vancouver:

Tan Y. Highly sensitive biosensors using photonic crystal enhanced fluorescence. [Internet] [Doctoral dissertation]. University of Illinois – Urbana-Champaign; 2015. [cited 2021 Jan 18]. Available from: http://hdl.handle.net/2142/73064.

Council of Science Editors:

Tan Y. Highly sensitive biosensors using photonic crystal enhanced fluorescence. [Doctoral Dissertation]. University of Illinois – Urbana-Champaign; 2015. Available from: http://hdl.handle.net/2142/73064

Louisiana State University

10. Peng, Zhiyong. Single-molecule detection of molecular beacons generated from LDR on thermoplastic microfluidic device for bioanalysis.

Degree: PhD, Chemistry, 2011, Louisiana State University

URL: etd-04272012-032346 ; https://digitalcommons.lsu.edu/gradschool_dissertations/843

► Current clinical techniques for nucleic acid detection and analysis often involve PCR, lacking adequate specificity or sensitivity to meet the stringent requirements in certain applications.… (more)

▼ Current clinical techniques for nucleic acid detection and analysis often involve PCR, lacking adequate specificity or sensitivity to meet the stringent requirements in certain applications. This research aims to develop an innovative molecular assay and the associated hardware to rapidly signal the presence of certain targets using reporter sequence found in their genome without requiring PCR. This assay coupled the sensitivity of single-pair fluorescence resonance energy transfer (spFRET) with the specificity of ligase detection reaction (LDR) to provide near real-time readout of target biomarkers. Heightened concerns on potential bioterrorism threats, such as rapid dissemination of pathogenic bacteria or viruses into water and/or food supplies, demand fast detection strategies. In this work, a pair of strain-specific primers was designed based on the 16S rRNA gene and were end-labeled with a donor (Cy5) and acceptor (Cy5.5) dyes. In the presence of the target bacterium, the primers were joined using LDR to form a reverse molecular beacon (rMB), thus bringing Cy5 and Cy5.5 into close proximity to allow FRET to occur. These rMBs were analyzed using single-molecule detection of the FRET pairs (spFRET). The LDR was performed in a Cyclic Olefin Copolymer (COC) microfluidic device equipped with 2 or 20 thermal cycles in a continuous flow format. Single-molecule photon bursts from the resulting rMBs were detected on-chip and registered using a laser-induced fluorescence (LIF) instrument. The presence of target pathogens could be reported in as little as 2.6 min using spFRET. In another development, a similar assay format was utilized to quantify mRNA expression levels of MMP-7 gene, which is highly relevant to invasion, metastasis and progression of a variety of tumors. HT-29 cells were found to express the highest levels of MMP-7 transcripts among the studied cell lines using LDR primers specific to MMP-7 gene. This observation is consistent with the results obtained with RT-qPCR. The LDR-spFRET assay was also used for stroke subtyping by designing primers specific to AMPH gene and using a microfluidic chip with tapered detection window to improve sampling efficiency. The detection could be completed in 15 min with extended readout time to glean low copy number transcripts.

Subjects/Keywords: molecular beacon; spFRET; LDR; microfluidic; single-molecule detection

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Peng, Z. (2011). Single-molecule detection of molecular beacons generated from LDR on thermoplastic microfluidic device for bioanalysis. (Doctoral Dissertation). Louisiana State University. Retrieved from etd-04272012-032346 ; https://digitalcommons.lsu.edu/gradschool_dissertations/843

Chicago Manual of Style (16^th Edition):

Peng, Zhiyong. “Single-molecule detection of molecular beacons generated from LDR on thermoplastic microfluidic device for bioanalysis.” 2011. Doctoral Dissertation, Louisiana State University. Accessed January 18, 2021. etd-04272012-032346 ; https://digitalcommons.lsu.edu/gradschool_dissertations/843.

MLA Handbook (7^th Edition):

Peng, Zhiyong. “Single-molecule detection of molecular beacons generated from LDR on thermoplastic microfluidic device for bioanalysis.” 2011. Web. 18 Jan 2021.

Vancouver:

Peng Z. Single-molecule detection of molecular beacons generated from LDR on thermoplastic microfluidic device for bioanalysis. [Internet] [Doctoral dissertation]. Louisiana State University; 2011. [cited 2021 Jan 18]. Available from: etd-04272012-032346 ; https://digitalcommons.lsu.edu/gradschool_dissertations/843.

Council of Science Editors:

Peng Z. Single-molecule detection of molecular beacons generated from LDR on thermoplastic microfluidic device for bioanalysis. [Doctoral Dissertation]. Louisiana State University; 2011. Available from: etd-04272012-032346 ; https://digitalcommons.lsu.edu/gradschool_dissertations/843

NSYSU

11. Lin, Jia-hui. Development of molecular- and nanomaterial-based probes for sensing biomolecules and metal ions.

Degree: PhD, Chemistry, 2015, NSYSU

URL: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0104115-131206

► The purpose of this thesis is to describe how to develop molecular- and nanomaterial-based biosensor for sensing biomolecules and metal ions. The thesis consists of… (more)

▼ The purpose of this thesis is to describe how to develop molecular- and nanomaterial-based biosensor for sensing biomolecules and metal ions. The thesis consists of four independent works. (1) A simple and label-free fluorescent method for sensitive and selective detection of adenosine in urine sample was developed based on adenosine-induced inhibition of adenosylhomocysteine hydrolase (SAHH) activity. Without the addition of nucleophile, 2,3-naphthalenedicarboxaldehyde (NDA) was found to selectively react with homocysteine to form fluorescent product. Adenosine is efficient to inhibit the production of homocysteine from the hydrolysis reaction between SAHH and adenosylhomocysteine. Taken together, the fluorescence of NDA-homocysteine derivatives decreased with an increase in the adenosine concentration. As a result, the SAHH-based probe provided high sensitivity (a limit of detection for adenosine of 0.3 Î¼M) and high selectivity (more than 100-fold for andenosine over any adenosine analogs). (2) A set of two-input and three-input DNA logic gates was constructed using non-Waston-Crick base pairing-based molecular beacon (MB). The presence of Hg2+, Ag+, and coralyne promoted the conformational changes of MB via the formation of T-Hg2+-T, C-Ag+-C, and A2-coralyne-A2 coordination, resulting in its fluorescent quenching. It was found that thiols, complexing agents, and polyadenosine can remove Hg2+, Ag+, and coralyne from the hairpin-shaped MB, respectively. Based on these phenomenon, the designed MB generated a series of two-input, three-input, and set-reset logic operation at the molecular level. (3) Laser-induced light scattering technique with the adevantages of high sensitivity, low cost, portability, and miniaturization was applied for sensing three gold nanoparticle-based sensing systems, including salt-, thiol-, and metal ion-induced nanoparticle aggregation. The combination of a miniatuarized spectrometer with a 532-nm laser allows the sensitive detection of Rayleigh scattering from nanoparticles aggregation. As a result, the proposed system provided more than 10-fold sensitivity improvement as compared to colorimetric assay. (4) Fluorescent polydopamine particles was prepared through hydroxyl-radical degradation of polydopamine nanoparticles. The production of fluorescent polydopamine was demonstrated using transmission electron microscope, dark field microscope, and thermogravimetric analyzer. The possible chemical compositions of polydopamine dots were determined using Fourier transform infrared spectrosmeter and laser desorption/ionization-time-of-flight mass spectrometer. Additionally, fluorescent polydopamine dots were used for sensitive and selective detection of Fe(III) through coordination between the catechol group of fluorescent polydopamine dots and Fe(III). This sensing mechanism relied on electron-transfer-induced fluorescence quenching. Advisors/Committee Members: Po-Chiao Lin (chair), Yang-Hsiang Chan (chair), Shou-Mei Wu (chair), Shiuh-jen Jiang (chair), Wei-Lung Tseng (committee member).

Subjects/Keywords: inhibition; derivatization; molecular logic gates; molecular beacon; gold nanoparticles; light scattering; fluorescent polydopamine

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APA (6^th Edition):

Lin, J. (2015). Development of molecular- and nanomaterial-based probes for sensing biomolecules and metal ions. (Doctoral Dissertation). NSYSU. Retrieved from http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0104115-131206

Chicago Manual of Style (16^th Edition):

Lin, Jia-hui. “Development of molecular- and nanomaterial-based probes for sensing biomolecules and metal ions.” 2015. Doctoral Dissertation, NSYSU. Accessed January 18, 2021. http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0104115-131206.

MLA Handbook (7^th Edition):

Lin, Jia-hui. “Development of molecular- and nanomaterial-based probes for sensing biomolecules and metal ions.” 2015. Web. 18 Jan 2021.

Vancouver:

Lin J. Development of molecular- and nanomaterial-based probes for sensing biomolecules and metal ions. [Internet] [Doctoral dissertation]. NSYSU; 2015. [cited 2021 Jan 18]. Available from: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0104115-131206.

Council of Science Editors:

Lin J. Development of molecular- and nanomaterial-based probes for sensing biomolecules and metal ions. [Doctoral Dissertation]. NSYSU; 2015. Available from: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0104115-131206

Université de Lorraine

12. Stallivieri, Aurélie. Synthèse de systèmes à base de photosensibilisateurs pour l'amélioration de la sélectivité tumorale en thérapie photodynamique : Synthesis of new systems based on photosensitizers for the improvement of the tumor selectivity in photodynamic therapy.

Degree: Docteur es, Génie des procédés et des produits, 2015, Université de Lorraine

URL: http://www.theses.fr/2015LORR0128

►

Une des limitations de la thérapie photodynamique est la faible sélectivité des photosensibilisateurs (PS) pour les tissus tumoraux. La recherche de nouveaux PS plus sélectifs… (more)

▼

Une des limitations de la thérapie photodynamique est la faible sélectivité des photosensibilisateurs (PS) pour les tissus tumoraux. La recherche de nouveaux PS plus sélectifs s’est orientée vers la synthèse de PS couplés à des motifs afins pour des récepteurs membranaires surexprimés dans certains cancers. Le récepteur à l’acide folique est surexprimé dans les carcinomes ovariens et des PS ont été conjugués à de l’acide folique. Des PS ont aussi été couplés à un peptide spécifique de neuropiline 1 surexprimé au niveau des cellules tumorales de médulloblastome. Une autre stratégie pour augmenter la sélectivité du traitement vise à produire les espèces réactives de l’oxygène spécifiquement au niveau du site tumoral. L’activité de clivage enzymatique de marqueurs biologiques surexprimés dans les zones tumorales est utilisée. Les gélatinases (MMP-2 et -9) et leur activateur MMP-14 sont connues pour jouer un rôle primordial dans l'angiogenèse tumorale et la croissance du glioblastome multiforme. Différents photodynamic molecular beacons (PMB), associant un PS et un quencher lié par un peptide substrat des gélatinases et MMP-14, ont été développés

One limitation of photodynamic therapy is the low selectivity of photosensitizers (PS) to tumour tissue. The search of new PS more selective began to focus on the synthesis of PS coupled with substrate specific of the membrane receptors overexpressed in certain cancers. The acid folic receptor is overexpressed in ovarian carcinomas and PS were conjugated with folic acid. PS were also coupled with a specific peptide of neuropilin 1 overexpressed in tumoral cells of medulloblastoma. Another strategy for increasing the selectivity of the treatment is to produce reactive oxygen species specifically at the tumor site. The activity of enzymatic cleavage of biomarkers overexpressed in tumour areas is used. The gelatinases (MMP-2 and MMP-9) and their activator MMP-14 are known to play a key role in tumour angiogenesis and the growth of glioblastoma multiform. Different photodynamic molecular beacons (PMB), composed of a photosensitizer and a quencher linked together by a peptide substrate of gelatinases or MMP-14, were designed.

Advisors/Committee Members: Frochot, Céline (thesis director), Chevalot, Isabelle (thesis director).

Subjects/Keywords: Sélectivité tumorale; Thérapie photodynamique; Photosensibilisateur; Photodynamic molecular beacon; Acide folique; Peptide; Tumor selectivity; Photodynamic therapy; Photosensitizer; Photodynamic molecular beacon; Folic acid; Peptide; 616.994 0631; 615.831

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Stallivieri, A. (2015). Synthèse de systèmes à base de photosensibilisateurs pour l'amélioration de la sélectivité tumorale en thérapie photodynamique : Synthesis of new systems based on photosensitizers for the improvement of the tumor selectivity in photodynamic therapy. (Doctoral Dissertation). Université de Lorraine. Retrieved from http://www.theses.fr/2015LORR0128

Chicago Manual of Style (16^th Edition):

Stallivieri, Aurélie. “Synthèse de systèmes à base de photosensibilisateurs pour l'amélioration de la sélectivité tumorale en thérapie photodynamique : Synthesis of new systems based on photosensitizers for the improvement of the tumor selectivity in photodynamic therapy.” 2015. Doctoral Dissertation, Université de Lorraine. Accessed January 18, 2021. http://www.theses.fr/2015LORR0128.

MLA Handbook (7^th Edition):

Stallivieri, Aurélie. “Synthèse de systèmes à base de photosensibilisateurs pour l'amélioration de la sélectivité tumorale en thérapie photodynamique : Synthesis of new systems based on photosensitizers for the improvement of the tumor selectivity in photodynamic therapy.” 2015. Web. 18 Jan 2021.

Vancouver:

Stallivieri A. Synthèse de systèmes à base de photosensibilisateurs pour l'amélioration de la sélectivité tumorale en thérapie photodynamique : Synthesis of new systems based on photosensitizers for the improvement of the tumor selectivity in photodynamic therapy. [Internet] [Doctoral dissertation]. Université de Lorraine; 2015. [cited 2021 Jan 18]. Available from: http://www.theses.fr/2015LORR0128.

Council of Science Editors:

Stallivieri A. Synthèse de systèmes à base de photosensibilisateurs pour l'amélioration de la sélectivité tumorale en thérapie photodynamique : Synthesis of new systems based on photosensitizers for the improvement of the tumor selectivity in photodynamic therapy. [Doctoral Dissertation]. Université de Lorraine; 2015. Available from: http://www.theses.fr/2015LORR0128

NSYSU

13. Lee, Chih-yi. Design fluorescent molecular beacon for sensing oversulfated chondroitin sulfate in pharmaceutical heparin and diadenosine analogs.

Degree: Master, Chemistry, 2015, NSYSU

URL: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0602115-150346

► (a) Molecular Beacon-Based Fluorescent Assay for Specific Detection of Oversulfated Chondroitin Sulfate Contaminants in Heparin without Enzyme Treatment Heparin belongs to a family of naturally… (more)

▼ (a) Molecular Beacon-Based Fluorescent Assay for Specific Detection of Oversulfated Chondroitin Sulfate Contaminants in Heparin without Enzyme Treatment Heparin belongs to a family of naturally occurring negatively charged glycosaminoglycans (GAGs) which are complex linear polysaccharides comprised of repeating disaccharide units. A sudden increase of severely adverse responses to heparin treatment of patients (including 94 deaths) was reported in U.S. during 2007-2008 period. The U.S. Food and Drug Administrationâs (FDA) investigation of this problem found that the origin of these fatal side effects was oversulfated chondroitin sulfate (OSCS) that was present in some heparin products. Here we presents a highly selective, sensitive, enzyme free, simple and fast method to detect OSCS in heparin. The MB (A8âMBâA8) contains a 22-mer loop, a pair of 8-mer adenosine (A) bases at stem, a fluorophore unit at the 5â-end, and a quencher at the 3â-end. Hairpin-shaped MB with A2-coralyne-A2 complex at stem has formed after addition of coralyne. This study found that with present of Ca2+ ions can efficiently suppress the negative charges of heparin, they do not neutralize the negative charge of OSCS, the result of OSCS can remove coralyne from the MB stem more than heparin. This system can detect 0.01% w/w OSCS in heparin without enzyme treatment, also determine 0.01% w/w OSCS in the pharmaceutical heparin under 5 min. â (b) A Simple Molecular Beacon-based Fluorescent Sensor for Sensing Second Messenger c-di-AMP and Signalling Molecular Ap5A c-di-AMP is an important bacterial second messenger found in Gram-positive and mycobacterial. Second messenger will enhancement, differentiation, integration and passed to the receptors to regulate physiological processes, in response to a changing environment. c-di-AMP is produced from two molecules of ATP by diadenylyl cyclase (DAC) enzyme, made up of two adenosine moieties joined in double 3â-5â linkage by two phosphodiester. Recently, we develop a simple molecular beacon-based senor for detection of c-di-AMP. The MB contains 20-mer adenosine (A) bases, a fluorophore unit (FAM) at the 5â-end, and a quencher (BHQ1) at the 3â-end. The presence of coralyne form a hairpin-shaped MB with A2-coralyne-A2 complex. After addition of c-di-AMP, it will compete with PolyA20 and displace coralyne out, and turn on fluorescence. Under optimal conditions (10nM MB, 0.5Î¼M coralyne), dtection limit (LOD) of c-di-AMP is 0.1Î¼M. Same mechanism is used in signaling molecular-Ap5A. Studies had demonstrated a ubiquitous occurrence of Ap5A in the whole spectrum of organisms from bacteria to higher eukaryotes, it is made up of two adenosine moieties joined in 5â-5â linkage by a chain of five phosphodiester linkages. The MB sensor can also detect Ap5A in tears sample, with detection limit of 3Î¼M. Advisors/Committee Members: Wei-Lung Tseng (committee member), Yang-Hsiang Chan (chair), Yu-Fen Hung (chair).

Subjects/Keywords: Second messenger; c-di-AMP; Heparin; Ap5A; Molecular beacon; Calcium; diadenosine; OSCS

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Lee, C. (2015). Design fluorescent molecular beacon for sensing oversulfated chondroitin sulfate in pharmaceutical heparin and diadenosine analogs. (Thesis). NSYSU. Retrieved from http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0602115-150346

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Lee, Chih-yi. “Design fluorescent molecular beacon for sensing oversulfated chondroitin sulfate in pharmaceutical heparin and diadenosine analogs.” 2015. Thesis, NSYSU. Accessed January 18, 2021. http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0602115-150346.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Lee, Chih-yi. “Design fluorescent molecular beacon for sensing oversulfated chondroitin sulfate in pharmaceutical heparin and diadenosine analogs.” 2015. Web. 18 Jan 2021.

Vancouver:

Lee C. Design fluorescent molecular beacon for sensing oversulfated chondroitin sulfate in pharmaceutical heparin and diadenosine analogs. [Internet] [Thesis]. NSYSU; 2015. [cited 2021 Jan 18]. Available from: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0602115-150346.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Lee C. Design fluorescent molecular beacon for sensing oversulfated chondroitin sulfate in pharmaceutical heparin and diadenosine analogs. [Thesis]. NSYSU; 2015. Available from: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0602115-150346

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

The Ohio State University

14. Zhou, Chenguang. NANOCARRIERS FOR THERAPEUTIC NUCLEIC ACID DELIVERY.

Degree: PhD, Pharmacy, 2012, The Ohio State University

URL: http://rave.ohiolink.edu/etdc/view?acc_num=osu1336584204

► The objective of this dissertation research is to develop nanocarrier (NC) systems for therapeutic nucleic acid delivery and to investigate the mechanism and kinetics of… (more)

Subjects/Keywords: Pharmaceuticals; nanoparticles; gene therapy; drug delivery; pharmacokinetics; siRNA; molecular beacon; intracellular trafficking

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Zhou, C. (2012). NANOCARRIERS FOR THERAPEUTIC NUCLEIC ACID DELIVERY. (Doctoral Dissertation). The Ohio State University. Retrieved from http://rave.ohiolink.edu/etdc/view?acc_num=osu1336584204

Chicago Manual of Style (16^th Edition):

Zhou, Chenguang. “NANOCARRIERS FOR THERAPEUTIC NUCLEIC ACID DELIVERY.” 2012. Doctoral Dissertation, The Ohio State University. Accessed January 18, 2021. http://rave.ohiolink.edu/etdc/view?acc_num=osu1336584204.

MLA Handbook (7^th Edition):

Zhou, Chenguang. “NANOCARRIERS FOR THERAPEUTIC NUCLEIC ACID DELIVERY.” 2012. Web. 18 Jan 2021.

Vancouver:

Zhou C. NANOCARRIERS FOR THERAPEUTIC NUCLEIC ACID DELIVERY. [Internet] [Doctoral dissertation]. The Ohio State University; 2012. [cited 2021 Jan 18]. Available from: http://rave.ohiolink.edu/etdc/view?acc_num=osu1336584204.

Council of Science Editors:

Zhou C. NANOCARRIERS FOR THERAPEUTIC NUCLEIC ACID DELIVERY. [Doctoral Dissertation]. The Ohio State University; 2012. Available from: http://rave.ohiolink.edu/etdc/view?acc_num=osu1336584204

NSYSU

15. Kuo, Chia-yin. Development of molecular beacon-based sensors for detecting heparin and triplex deoxyribonucleic acid binders.

Degree: Master, Chemistry, 2013, NSYSU

URL: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0619113-005536

None Advisors/Committee Members: Wei-Lung Tseng (committee member), Chih-Hsin Chen (chair), Yang-Hsiang Chan (chair).

Subjects/Keywords: Molecular beacon; Triplex DNA binders; Heparin; Coralyne

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APA (6^th Edition):

Kuo, C. (2013). Development of molecular beacon-based sensors for detecting heparin and triplex deoxyribonucleic acid binders. (Thesis). NSYSU. Retrieved from http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0619113-005536

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Kuo, Chia-yin. “Development of molecular beacon-based sensors for detecting heparin and triplex deoxyribonucleic acid binders.” 2013. Thesis, NSYSU. Accessed January 18, 2021. http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0619113-005536.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Kuo, Chia-yin. “Development of molecular beacon-based sensors for detecting heparin and triplex deoxyribonucleic acid binders.” 2013. Web. 18 Jan 2021.

Vancouver:

Kuo C. Development of molecular beacon-based sensors for detecting heparin and triplex deoxyribonucleic acid binders. [Internet] [Thesis]. NSYSU; 2013. [cited 2021 Jan 18]. Available from: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0619113-005536.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Kuo C. Development of molecular beacon-based sensors for detecting heparin and triplex deoxyribonucleic acid binders. [Thesis]. NSYSU; 2013. Available from: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0619113-005536

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

16. Jakir Ahmed, Chowdhury. Development of New Fluorogenic OligoDNA Probes Utilizing Silylated Fluorescent Material : シリル化された蛍光物質を用いた新規な蛍光発光性オリゴDNA プローブの開発.

Degree: 博士（工学）, 2015, Gunma University / 群馬大学

URL: http://hdl.handle.net/10087/8987

学位記番号:工博甲494

Subjects/Keywords: OligoDNA Probe; Silylated Fluorescent Material; Molecular beacon

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Jakir Ahmed, C. (2015). Development of New Fluorogenic OligoDNA Probes Utilizing Silylated Fluorescent Material : シリル化された蛍光物質を用いた新規な蛍光発光性オリゴDNA プローブの開発. (Thesis). Gunma University / 群馬大学. Retrieved from http://hdl.handle.net/10087/8987

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Jakir Ahmed, Chowdhury. “Development of New Fluorogenic OligoDNA Probes Utilizing Silylated Fluorescent Material : シリル化された蛍光物質を用いた新規な蛍光発光性オリゴDNA プローブの開発.” 2015. Thesis, Gunma University / 群馬大学. Accessed January 18, 2021. http://hdl.handle.net/10087/8987.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Jakir Ahmed, Chowdhury. “Development of New Fluorogenic OligoDNA Probes Utilizing Silylated Fluorescent Material : シリル化された蛍光物質を用いた新規な蛍光発光性オリゴDNA プローブの開発.” 2015. Web. 18 Jan 2021.

Vancouver:

Jakir Ahmed C. Development of New Fluorogenic OligoDNA Probes Utilizing Silylated Fluorescent Material : シリル化された蛍光物質を用いた新規な蛍光発光性オリゴDNA プローブの開発. [Internet] [Thesis]. Gunma University / 群馬大学; 2015. [cited 2021 Jan 18]. Available from: http://hdl.handle.net/10087/8987.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Jakir Ahmed C. Development of New Fluorogenic OligoDNA Probes Utilizing Silylated Fluorescent Material : シリル化された蛍光物質を用いた新規な蛍光発光性オリゴDNA プローブの開発. [Thesis]. Gunma University / 群馬大学; 2015. Available from: http://hdl.handle.net/10087/8987

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Alberta

17. El-Yazbi, Amira F. Novel Spectroscopic Probes for Detecting DNA Damage.

Degree: PhD, Department of Chemistry, 2013, University of Alberta

URL: https://era.library.ualberta.ca/files/hm50tt27s

► Absorption of UV light by nucleic acids can result in the formation of molecular lesions leading to mutagenesis, carcinogenesis, and cell death. Thus, understanding DNA… (more)

▼ Absorption of UV light by nucleic acids can result in the formation of molecular lesions leading to mutagenesis, carcinogenesis, and cell death. Thus, understanding DNA damage is important for elucidating the molecular mechanisms of disease. Much effort has been focused on developing methods for detecting DNA damage. However, almost all of the proposed methods consist of multi-step procedures, are limited to a specific type of damage, require expensive instruments, and/or suffer from a high level of interference. In this thesis, I present some novel simple, mix-and-read fluorescent assays for the detection of DNA damage. The goal is to design probes that are superior to conventional fluorescent molecular beacons (MBs) in detecting DNA damage. The first approach was to design MBs with modified DNA backbones. For this purpose, locked nucleic acid (LNA) and chimeric RNA-DNA (chMB) MBs were designed. The results show that chMBs are more sensitive and selective for DNA damage than LNA MBs that have comparable selectivity to conventional MBs. However, these probes show a signal that is inversely proportional to the amount of damage. Therefore, the second approach was to design probes that give signals directly proportional to the amount of damage. For this purpose, probes with 2-aminopurine (2AP) were designed. Such probes show no fluorescence for undamaged DNA and fluorescence for damaged DNA. 2AP probes offer high sensitivity and selectivity comparable to MBs, but are expensive, especially with an increasing number of 2APs in the probe to increase sensitivity. Thus, the hypochromism probe was designed. For this probe, the absorbance signal increases with increasing amount of damage. Results show that the hypochromism probe is more selective and more than ten times cheaper than conventional MBs, but less sensitive. The need for a sensitive, selective and inexpensive probe was the motivation to design the Tb3+/hairpin probe. Single-stranded DNA greatly enhances the Tb3+ emission, but duplex DNA does not. Undamaged DNA targets will hybridize with the hairpin with no emission. The Tb3+/DNA hairpin probe proves to be the cheapest, most sensitive and selective probe for the quantification of DNA damage of all the probes presented here.

Subjects/Keywords: Fluorescence; Terbium; Chimeric RNA DNA probe; LNA probe; Hypochromism; 2-aminopurine; Nucleic acid detection; Hybridization; Ultraviolet light; Molecular beacon; DNA damage

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APA (6^th Edition):

El-Yazbi, A. F. (2013). Novel Spectroscopic Probes for Detecting DNA Damage. (Doctoral Dissertation). University of Alberta. Retrieved from https://era.library.ualberta.ca/files/hm50tt27s

Chicago Manual of Style (16^th Edition):

El-Yazbi, Amira F. “Novel Spectroscopic Probes for Detecting DNA Damage.” 2013. Doctoral Dissertation, University of Alberta. Accessed January 18, 2021. https://era.library.ualberta.ca/files/hm50tt27s.

MLA Handbook (7^th Edition):

El-Yazbi, Amira F. “Novel Spectroscopic Probes for Detecting DNA Damage.” 2013. Web. 18 Jan 2021.

Vancouver:

El-Yazbi AF. Novel Spectroscopic Probes for Detecting DNA Damage. [Internet] [Doctoral dissertation]. University of Alberta; 2013. [cited 2021 Jan 18]. Available from: https://era.library.ualberta.ca/files/hm50tt27s.

Council of Science Editors:

El-Yazbi AF. Novel Spectroscopic Probes for Detecting DNA Damage. [Doctoral Dissertation]. University of Alberta; 2013. Available from: https://era.library.ualberta.ca/files/hm50tt27s

18. Moutsiopoulou, Angeliki. Design of Bioluminescent Protein-Conjugates with Bioorthogonal Chemistry for Applications in Biomedicine.

Degree: PhD, Chemistry (Arts and Sciences), 2019, University of Miami

URL: https://scholarlyrepository.miami.edu/oa_dissertations/2295

► Bioconjugation is one of the common ways of modifying biomolecules with reporters and other moieties for applications in the development of various sensing systems. However,… (more)

Subjects/Keywords: bioorthogonal chemistry; immunoassay; molecular aptamer beacon; bioluminescence

…aptamer beacon ................... 20 Working Principle of Molecular Aptamer Beacon ......... 23… …71 3 Bioluminescent Protein-Inhibitor Pair in the Design of a Molecular Aptamer Beacon… …77 3.2.1 Development of Molecular Aptamer Beacon… …90 3.4.5 Synthesis of the conjugate molecular aptamer beacon with inhibitor ... 91 3.4.6… …93 4.2 Different protein bioorthogonal conjugation with the molecular aptamer beacon…

10 more images…

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APA (6^th Edition):

Moutsiopoulou, A. (2019). Design of Bioluminescent Protein-Conjugates with Bioorthogonal Chemistry for Applications in Biomedicine. (Doctoral Dissertation). University of Miami. Retrieved from https://scholarlyrepository.miami.edu/oa_dissertations/2295

Chicago Manual of Style (16^th Edition):

Moutsiopoulou, Angeliki. “Design of Bioluminescent Protein-Conjugates with Bioorthogonal Chemistry for Applications in Biomedicine.” 2019. Doctoral Dissertation, University of Miami. Accessed January 18, 2021. https://scholarlyrepository.miami.edu/oa_dissertations/2295.

MLA Handbook (7^th Edition):

Moutsiopoulou, Angeliki. “Design of Bioluminescent Protein-Conjugates with Bioorthogonal Chemistry for Applications in Biomedicine.” 2019. Web. 18 Jan 2021.

Vancouver:

Moutsiopoulou A. Design of Bioluminescent Protein-Conjugates with Bioorthogonal Chemistry for Applications in Biomedicine. [Internet] [Doctoral dissertation]. University of Miami; 2019. [cited 2021 Jan 18]. Available from: https://scholarlyrepository.miami.edu/oa_dissertations/2295.

Council of Science Editors:

Moutsiopoulou A. Design of Bioluminescent Protein-Conjugates with Bioorthogonal Chemistry for Applications in Biomedicine. [Doctoral Dissertation]. University of Miami; 2019. Available from: https://scholarlyrepository.miami.edu/oa_dissertations/2295

Louisiana State University

19. Ghosn, Bilal. Photo-control of DNA oligonucleotides with cage compounds.

Degree: MSBAE, Engineering, 2004, Louisiana State University

URL: etd-11102004-161129 ; https://digitalcommons.lsu.edu/gradschool_theses/4095

► Many biochemical processes in which DNA and other nucleic acids participate are central to functions in both living cells and in molecular biology assays. While… (more)

▼ Many biochemical processes in which DNA and other nucleic acids participate are central to functions in both living cells and in molecular biology assays. While many compounds have been used to regulate the activity of DNA, these strategies are limited to the aqueous-based diffusion of the activator to the target DNA molecule. An improvement to the induction of DNA bioactivity is to move to a light-based modulation. This research demonstrates a light-based technique using a photo-cleavable cage compound to transiently inactivate DNA hybridization. Function can be restored with exposure to near-UV light, allowing for temporal control of DNA oligonucleotide (ODN) activity. This method has demonstrated the control of hybridization in molecular biology assays, and provides the framework for in vivo experimentation. A similar light-activated strategy has been shown useful in controlling expression of plasmid transgenes (Monroe 1999). By adapting this method to DNA oligonucleotides (ODNs), we have partially blocked hybridization with the cage compound (1-(4,5-dimethoxy-2-nitrophenyl)ethyl ester (DMNPE) for both phosphodiester and phosphorothioate DNA ODNs. The production and purification of DMNPE-caged DNA ODNs yields products with similar spectrophotometric properties to caged plasmids. In hybridization studies, 20-mer (20 base long) caged DNA ODNs were hybridized with complementary 30-mer molecular beacon probes, and fluorescence measurements were used to assess hybridization of native (non-caged), caged, and caged-light-exposed ODNs. Developments of the molecular beacon assays were studied to improve sensitivity of the assay to caged and caged-flashed ODN hybridization control. Results demonstrated that hybridization can be blocked and subsequently restored by light through the attachment of the DMNPE cage compound, and were further characterized with gel electrophoresis assays. ODN hybridization was restricted to as little as 2% when compared to native (non-caged) ODNs and restored to up to nearly 80% of the native (non-caged) ODN hybridization activity levels. Additional studies on adduction, purification, and characterization of the DMNPE-caged ODNs were performed to optimize their production and efficacy in controlling hybridization. These results suggest that this light-based technology can be used as a tool for the spatial and temporal regulation of hybridization-based DNA bioactivity, including applications with antisense ODNs as a form of controlled gene therapy.

Subjects/Keywords: molecular beacon; oligonucleotide; hybridization; uv-light; caged compounds

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APA (6^th Edition):

Ghosn, B. (2004). Photo-control of DNA oligonucleotides with cage compounds. (Masters Thesis). Louisiana State University. Retrieved from etd-11102004-161129 ; https://digitalcommons.lsu.edu/gradschool_theses/4095

Chicago Manual of Style (16^th Edition):

Ghosn, Bilal. “Photo-control of DNA oligonucleotides with cage compounds.” 2004. Masters Thesis, Louisiana State University. Accessed January 18, 2021. etd-11102004-161129 ; https://digitalcommons.lsu.edu/gradschool_theses/4095.

MLA Handbook (7^th Edition):

Ghosn, Bilal. “Photo-control of DNA oligonucleotides with cage compounds.” 2004. Web. 18 Jan 2021.

Vancouver:

Ghosn B. Photo-control of DNA oligonucleotides with cage compounds. [Internet] [Masters thesis]. Louisiana State University; 2004. [cited 2021 Jan 18]. Available from: etd-11102004-161129 ; https://digitalcommons.lsu.edu/gradschool_theses/4095.

Council of Science Editors:

Ghosn B. Photo-control of DNA oligonucleotides with cage compounds. [Masters Thesis]. Louisiana State University; 2004. Available from: etd-11102004-161129 ; https://digitalcommons.lsu.edu/gradschool_theses/4095

20. 佐々木, 淳. 生体内核酸イメージングに向けたフッ素ラベル化合物開発.

Degree: Japan Advanced Institute of Science and Technology / 北陸先端科学技術大学院大学

URL: http://hdl.handle.net/10119/9721

Supervisor:藤本　健造

マテリアルサイエンス研究科

修士

Subjects/Keywords: 19F MRI; 19F MRI; イメージングプローブ; imaging probe; モレキュラービーコン; molecular beacon

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

佐々木, �. (n.d.). 生体内核酸イメージングに向けたフッ素ラベル化合物開発. (Thesis). Japan Advanced Institute of Science and Technology / 北陸先端科学技術大学院大学. Retrieved from http://hdl.handle.net/10119/9721

Note: this citation may be lacking information needed for this citation format:
No year of publication.
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

佐々木, 淳. “生体内核酸イメージングに向けたフッ素ラベル化合物開発.” Thesis, Japan Advanced Institute of Science and Technology / 北陸先端科学技術大学院大学. Accessed January 18, 2021. http://hdl.handle.net/10119/9721.

Note: this citation may be lacking information needed for this citation format:
No year of publication.
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

佐々木, 淳. “生体内核酸イメージングに向けたフッ素ラベル化合物開発.” Web. 18 Jan 2021.

Note: this citation may be lacking information needed for this citation format:
No year of publication.

Vancouver:

佐々木 �. 生体内核酸イメージングに向けたフッ素ラベル化合物開発. [Internet] [Thesis]. Japan Advanced Institute of Science and Technology / 北陸先端科学技術大学院大学; [cited 2021 Jan 18]. Available from: http://hdl.handle.net/10119/9721.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
No year of publication.

Council of Science Editors:

佐々木 �. 生体内核酸イメージングに向けたフッ素ラベル化合物開発. [Thesis]. Japan Advanced Institute of Science and Technology / 北陸先端科学技術大学院大学; Available from: http://hdl.handle.net/10119/9721

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
No year of publication.

21. Reano, Dane Charles. Characterizing the Inactivation Profile of BK Polyomavirus Through a Molecular Beacon Assay.

Degree: Microbiology, 2016, University of California – Riverside

URL: http://www.escholarship.org/uc/item/8xr4t45t

► Regulations set forth by the United States Environmental Protection Agency (EPA) have significantly elevated standards for water treatment by ultraviolet light irradiation (UV). These standards… (more)

Subjects/Keywords: Virology; Water resources management; Environmental science; Inactivation; Molecular Beacon; Polyomavirus

…15-19 II. CHAPTER TWO: BK POLYOMAVIRUS DETECTION BY MOLECULAR BEACON ABSTRACT… …ADENOVIRUS 2, AND POLIOVIRUS 1 BY MOLECULAR BEACON ABSTRACT… …MOLECULAR BEACON ABSTRACT… …13 Figure 1.2 Molecular beacon structure and hybridization… …Surface Water Treatment Rule MB, molecular beacon xi MEM, Minimum Essential Medium N, titer…

11 more images…

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APA (6^th Edition):

Reano, D. C. (2016). Characterizing the Inactivation Profile of BK Polyomavirus Through a Molecular Beacon Assay. (Thesis). University of California – Riverside. Retrieved from http://www.escholarship.org/uc/item/8xr4t45t

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Reano, Dane Charles. “Characterizing the Inactivation Profile of BK Polyomavirus Through a Molecular Beacon Assay.” 2016. Thesis, University of California – Riverside. Accessed January 18, 2021. http://www.escholarship.org/uc/item/8xr4t45t.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Reano, Dane Charles. “Characterizing the Inactivation Profile of BK Polyomavirus Through a Molecular Beacon Assay.” 2016. Web. 18 Jan 2021.

Vancouver:

Reano DC. Characterizing the Inactivation Profile of BK Polyomavirus Through a Molecular Beacon Assay. [Internet] [Thesis]. University of California – Riverside; 2016. [cited 2021 Jan 18]. Available from: http://www.escholarship.org/uc/item/8xr4t45t.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Reano DC. Characterizing the Inactivation Profile of BK Polyomavirus Through a Molecular Beacon Assay. [Thesis]. University of California – Riverside; 2016. Available from: http://www.escholarship.org/uc/item/8xr4t45t

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

22. CHEN JIANBIN. DEVELOPMENT OF NEW TOOLBOXES FOR RAPID DETECTION OF SUBTLE DNA ALTERATIONS AND FOR VISUALIZATION OF RNA DYNAMICS.

Degree: 2015, National University of Singapore

URL: http://scholarbank.nus.edu.sg/handle/10635/122305

Subjects/Keywords: developmental genetics; gene editing; indel detection; molecular beacon; morpholino; RNA visualization

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

JIANBIN, C. (2015). DEVELOPMENT OF NEW TOOLBOXES FOR RAPID DETECTION OF SUBTLE DNA ALTERATIONS AND FOR VISUALIZATION OF RNA DYNAMICS. (Thesis). National University of Singapore. Retrieved from http://scholarbank.nus.edu.sg/handle/10635/122305

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

JIANBIN, CHEN. “DEVELOPMENT OF NEW TOOLBOXES FOR RAPID DETECTION OF SUBTLE DNA ALTERATIONS AND FOR VISUALIZATION OF RNA DYNAMICS.” 2015. Thesis, National University of Singapore. Accessed January 18, 2021. http://scholarbank.nus.edu.sg/handle/10635/122305.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

JIANBIN, CHEN. “DEVELOPMENT OF NEW TOOLBOXES FOR RAPID DETECTION OF SUBTLE DNA ALTERATIONS AND FOR VISUALIZATION OF RNA DYNAMICS.” 2015. Web. 18 Jan 2021.

Vancouver:

JIANBIN C. DEVELOPMENT OF NEW TOOLBOXES FOR RAPID DETECTION OF SUBTLE DNA ALTERATIONS AND FOR VISUALIZATION OF RNA DYNAMICS. [Internet] [Thesis]. National University of Singapore; 2015. [cited 2021 Jan 18]. Available from: http://scholarbank.nus.edu.sg/handle/10635/122305.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

JIANBIN C. DEVELOPMENT OF NEW TOOLBOXES FOR RAPID DETECTION OF SUBTLE DNA ALTERATIONS AND FOR VISUALIZATION OF RNA DYNAMICS. [Thesis]. National University of Singapore; 2015. Available from: http://scholarbank.nus.edu.sg/handle/10635/122305

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Université de Lorraine

23. Zaghdoudi, Khalil. Optimisation de l’extraction des caroténoïdes à partir du persimmon (Diospyros kaki L.), de l’abricot (Prunus armeniaca L.) et de la pêche (Prunus persica L.) : étude photophysique en vue d’une application en thérapie photodynamique (PDT) : Optimization of carotenoids extraction from persimmon (Diospyros kaki L.), apricot (Prunus armeniaca L.) and peach (Prunus persica L.) : Photophysical study for photodynamic therapy (PDT) application.

Degree: Docteur es, Génie des procédés et des produits, 2015, Université de Lorraine

URL: http://www.theses.fr/2015LORR0297

► La thérapie photodynamique (PDT) est une technique utilisée cliniquement pour traiter certaines maladies de la peau, la dégénérescence maculaire liée à l’âge et certains types… (more)

▼ La thérapie photodynamique (PDT) est une technique utilisée cliniquement pour traiter certaines maladies de la peau, la dégénérescence maculaire liée à l’âge et certains types de cancer. Elle fait intervenir trois composants : une molécule photosensible ou photosensibilisateur (PS), la lumière et l’oxygène. Après administration du PS, celui-ci va se localiser plus ou moins sélectivement dans les zones tumorales où il est alors activé par irradiation lumineuse à une longueur d’onde et une puissance données. Ceci engendre la formation d’espèces réactives de l’oxygène (ROS) très réactives, dont l'oxygène singulet1 O2, qui entraînent la destruction des tissus tumoraux par nécrose ou apoptose. Afin d’améliorer la sélectivité du traitement, différentes pistes sont actuellement exploitées dont l’élaboration de « photodynamic molecular beacons » (PMB). Dans un PMB, le photosensibilisateur (PS) est associé via un peptide à un inhibiteur 1O2, appelé quencher. Ce quencher inhibe la formation d’1O2 tant que le composé n’a pas atteint sa cible. Une fois la zone cancéreuse atteinte, des enzymes spécifiques clivent le peptide, libérant ainsi le PS qui retrouve alors sa capacité à former de l’1O2. Trouver un couple PS/quencher adéquat reste un challenge en PDT. Les propriétés photophysiques particulières des caroténoïdes et leur aptitude à inhiber la production d’1O2 font de ces derniers des quenchers potentiellement utilisables pour l’élaboration de PMBs. Chez les plantes, les caroténoïdes (carotènes et xanthophylles) sont des pigments associés à la photosynthèse, qui ont deux rôles principaux : un rôle de collecteur de lumière et un rôle photoprotecteur en protégeant le(s) système(s) photosynthétique(s) contre les dommages photooxydatifs liés à une exposition trop intense à la lumière. Ceci s’opère, entre autre, via le cycle des xanthophylles. Cette aptitude à capter de l’énergie présente un intérêt potentiel à ne pas négliger dans la perspective de la conception de PMB utilisables en thérapie photodynamique. Dans le cadre de cette thèse en co-tutelle avec la Faculté des Sciences de Bizerte nous avons ciblé les caroténoïdes présents dans trois fruits produits en Tunisie à savoir les kakis (Diospyros kaki L.), les abricots (Prunus armeniaca L.) et les pêches (Prunus persica L.) connus pour leur richesse globale en ces pigments. Divers procédés d’extractions ont été étudiés : (i) L’extraction de type Soxhlet par solvants organiques à pression atmosphérique, utilisée comme référence, (ii) l'extraction accélérée par solvant organique (ASE : Accelerated solvent Extraction) effectuée sous pression, enfin (iii) l'extraction par CO2 supercritique avec l’éthanol comme cosolvant. Pour ces deux derniers procédés, une approche par plan d’expériences (surfaces de réponses) a été utilisée pour identifier les facteurs clé et les conditions optimales d’extractions de divers caroténoïdes (pression, température, débit, % de cosolvant, temps, nombre de cycles). L'analyse par chromatographie liquide à haute performance couplée à la détection… Advisors/Committee Members: Frochot, Céline (thesis director).

Subjects/Keywords: Thérapie photodynamique (PDT); Cancer; Fruits; Caroténoïdes; Photodynamic molecular beacon (PMB); Oxygène singulet; Extraction par CO2 supercritique; Extraction accélérée par solvant organique sous pression; Quenching; Fluorescence; Photodynamic therapy (PDT); Cancer; Fruits; Carotenoids; Photodynamic molecular beacon (PMB); Singlet oxygen; Quenching; Fluorescence; Accelerated solvent extraction (ASE); Supercritical Fluid Extraction (SFE); 664.022; 572.592

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Zaghdoudi, K. (2015). Optimisation de l’extraction des caroténoïdes à partir du persimmon (Diospyros kaki L.), de l’abricot (Prunus armeniaca L.) et de la pêche (Prunus persica L.) : étude photophysique en vue d’une application en thérapie photodynamique (PDT) : Optimization of carotenoids extraction from persimmon (Diospyros kaki L.), apricot (Prunus armeniaca L.) and peach (Prunus persica L.) : Photophysical study for photodynamic therapy (PDT) application. (Doctoral Dissertation). Université de Lorraine. Retrieved from http://www.theses.fr/2015LORR0297

Chicago Manual of Style (16^th Edition):

Zaghdoudi, Khalil. “Optimisation de l’extraction des caroténoïdes à partir du persimmon (Diospyros kaki L.), de l’abricot (Prunus armeniaca L.) et de la pêche (Prunus persica L.) : étude photophysique en vue d’une application en thérapie photodynamique (PDT) : Optimization of carotenoids extraction from persimmon (Diospyros kaki L.), apricot (Prunus armeniaca L.) and peach (Prunus persica L.) : Photophysical study for photodynamic therapy (PDT) application.” 2015. Doctoral Dissertation, Université de Lorraine. Accessed January 18, 2021. http://www.theses.fr/2015LORR0297.

MLA Handbook (7^th Edition):

Zaghdoudi, Khalil. “Optimisation de l’extraction des caroténoïdes à partir du persimmon (Diospyros kaki L.), de l’abricot (Prunus armeniaca L.) et de la pêche (Prunus persica L.) : étude photophysique en vue d’une application en thérapie photodynamique (PDT) : Optimization of carotenoids extraction from persimmon (Diospyros kaki L.), apricot (Prunus armeniaca L.) and peach (Prunus persica L.) : Photophysical study for photodynamic therapy (PDT) application.” 2015. Web. 18 Jan 2021.

Vancouver:

Zaghdoudi K. Optimisation de l’extraction des caroténoïdes à partir du persimmon (Diospyros kaki L.), de l’abricot (Prunus armeniaca L.) et de la pêche (Prunus persica L.) : étude photophysique en vue d’une application en thérapie photodynamique (PDT) : Optimization of carotenoids extraction from persimmon (Diospyros kaki L.), apricot (Prunus armeniaca L.) and peach (Prunus persica L.) : Photophysical study for photodynamic therapy (PDT) application. [Internet] [Doctoral dissertation]. Université de Lorraine; 2015. [cited 2021 Jan 18]. Available from: http://www.theses.fr/2015LORR0297.

Council of Science Editors:

Zaghdoudi K. Optimisation de l’extraction des caroténoïdes à partir du persimmon (Diospyros kaki L.), de l’abricot (Prunus armeniaca L.) et de la pêche (Prunus persica L.) : étude photophysique en vue d’une application en thérapie photodynamique (PDT) : Optimization of carotenoids extraction from persimmon (Diospyros kaki L.), apricot (Prunus armeniaca L.) and peach (Prunus persica L.) : Photophysical study for photodynamic therapy (PDT) application. [Doctoral Dissertation]. Université de Lorraine; 2015. Available from: http://www.theses.fr/2015LORR0297

University of Vienna

24. Rizzi, Matthias. Genome release of human rhinovirus using molecular beacons.

Degree: 2009, University of Vienna

URL: http://othes.univie.ac.at/6810/

►

Die vorliegende Arbeit bedient sich einer neuen Methode der DNA/RNA Erkennung, der Molecualr Beacon Sonde. Ein Molecular Beacon ist in der Lage eine bestimmte DNA… (more)

▼

Die vorliegende Arbeit bedient sich einer neuen Methode der DNA/RNA Erkennung, der Molecualr Beacon Sonde. Ein Molecular Beacon ist in der Lage eine bestimmte DNA bzw. RNA Sequenz zu erkennen und spezifisch an diese zu binden. In Folge der Hybridisierung von Sonde und DNA/RNA untergeht das Molecular Beacon eine strukturelle Änderung und beginnt zu fluoreszieren. Die Absicht dieses wissenschaftlichen Projekts war es eine Molecular Beacon Sonde zu designen, die in der Lage ist mit viraler RNA des humanen Rhinoviruses 2 zu hybridisieren und diese Methode für Virusanalysen auf der Kapillarelektrophorese anzuwenden. Human Rhinoviren sind der pathogene Erreger für die allgemeine Verkühlung im menschlichen Körper und daher der Grund für eine der häufigsten Infektionen überhaupt. Design, Charakterisierung und Anwendung des Molecular Beacons ACGC zur Erkennung viraler RNA sind beschrieben. Schmelzexperimente enthüllen einen starken Einfluß der Mg2+ und einen schwächeren Einfluß der Na+ Konzentration auf de Schmelzeigenschaften des Molecular Beacons. In batch Experimente beweisen, dass das Molecular Beacon auf Grund der Hybridisierung sich öffnet, was in einem sechsfachen Anstieg der Fluoreszenz zu sehen ist. Ergebnisse der kapillarelektrophoretischen Experimente mit Laser induzierter Fluoreszenzdetektion zeigen sogar einen bis zu hundertfachen Anstieg der Fluorezenz für den Hybrid. Einiger Schwächen dieser Methode zum Trotz konnte gezeigt werden, das dieses neue Werkzeug der bioanalytischen Chemie funktioniert , weitere Untersuchungen zur Verbesserung der Zuverlässigkeit dieser Methode im Speziellen für kapillarphoretische Experimente jedoch gemacht werden müssen.

The present work deals with a new method for DNA/ RNA recognition, the Molecular Beacon probe. A Molecular Beacon is able to recognize and bind to a specific DNA or RNA target sequence. Due to this hybridization of the probe and the target, the Beacon undergoes a conformational change and starts fluorescing. The aim of this scientific project was to design a Molecular Beacon probe that is able to hybrid-ize upon viral RNA of the Human Rhinovirus 2 and to apply this method for virus analysis by capillary electrophoresis. Human Rhinoviruses are the pathogen agent for the common cold in the human body and therefore the cause for one of the most frequent infections at all. Design, characterization and application of the Molecular Beacon ACGC for viral RNA recogni-tion are described. Melting experiments reveal a strong influence of Mg2+ and a smaller influ-ence of Na+ concentration on melting properties of the Molecular Beacon. In batch experiments prove that the Molecular Beacon opens due to hybridization, resulting in up to 6-fold increase of fluorescence. Results of Capillary Electrophoresis experiments with Laser Induced Fluorescence detection even show an up to 100fold increase of fluorescence light for the probe-target-hybrid. Despite some weaknesses of this method it could be shown that this novel tool for bioanalytical chemistry works, but further…

Subjects/Keywords: 35.39 Analytische Chemie: Sonstiges; Molecular Beacon / Kappillarelektrophorese / humaner Rhinovirus / RNA Freisetzung / Fluoreszenz / UV; Molecular Beacon / capillar electrophoresis / human rhinovirus / RNA release / fluorescence / UV

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Rizzi, M. (2009). Genome release of human rhinovirus using molecular beacons. (Thesis). University of Vienna. Retrieved from http://othes.univie.ac.at/6810/

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Rizzi, Matthias. “Genome release of human rhinovirus using molecular beacons.” 2009. Thesis, University of Vienna. Accessed January 18, 2021. http://othes.univie.ac.at/6810/.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Rizzi, Matthias. “Genome release of human rhinovirus using molecular beacons.” 2009. Web. 18 Jan 2021.

Vancouver:

Rizzi M. Genome release of human rhinovirus using molecular beacons. [Internet] [Thesis]. University of Vienna; 2009. [cited 2021 Jan 18]. Available from: http://othes.univie.ac.at/6810/.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Rizzi M. Genome release of human rhinovirus using molecular beacons. [Thesis]. University of Vienna; 2009. Available from: http://othes.univie.ac.at/6810/

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Vienna

25. Weiss, Victor. Chip electrophoresis of human rhinovirus and receptor decorated liposomes as model membranes for the analysis of key steps in the viral infection pathway.

Degree: 2009, University of Vienna

URL: http://othes.univie.ac.at/7576/

►

Bis heute konnten in etwa 100 Serotypen von Humanen Rhinoviren (HRVs), welche eine der häufigsten Erkältungsursachen darstellen, unterschieden werden. Diese Serotypen werden nach dem jeweiligen… (more)

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Bis heute konnten in etwa 100 Serotypen von Humanen Rhinoviren (HRVs), welche eine der häufigsten Erkältungsursachen darstellen, unterschieden werden. Diese Serotypen werden nach dem jeweiligen Rezeptor, an den sie im Laufe der Zellinfektion binden, in zwei Gruppen unterteilt. Viren der Hauptgruppe binden an intercellular adhesion molecule 1 (ICAM – 1), Viren der Nebengruppe an Vertreter der low-density-lipoprotein Rezeptor (LDLR) Familie. Chip Elektrophorese ermöglichte in der Vergangenheit bereits erfolgreich die Analyse von fluoreszenzmarkierten Viren sowie die Verfolgung entsprechender Bioaffinitätsreaktionen. Zum Beispiel konnte die Bindung von HRV2, einem Rhinovirus der Nebengruppe, an Rezeptorfragmente verfolgt werden [Weiss et al. (2007): Virus analysis by electrophoresis on a microfluidic chip, J. Chromatogr. B 860, 173 – 179 sowie Kolivoška et al. (2007): Electrophoresis on a microfluidic chip for analysis of fluorescence-labeled human rhinovirus, Electrophoresis 28, 4734 – 4740]. In Weiterführung dieser grundlegenden Experimente, versuchte ich, im Laufe dieser Dissertation, die spezifische, rezeptorvermittelte Bindung von fluoreszenzmarkiertem HRV2 an Zellen mit Hilfe von Chip Elektrophorese zu zeigen. Anstelle von Zellen arbeitete ich jedoch mit unilamellaren, rezeptordekorierten Vesikeln (Liposomen). Dieses Modellsystem ermöglichte Experimente unter gut definierten Bedingungen, anders als es bei Verwendung von tatsächlichem zellulären Material möglich gewesen wäre. Zusätzlich zu reinen Bindungsexperimenten zwischen Viren und Membranen wurde auch der Transfer der viralen RNA in das Liposomeninnere untersucht. In vivo gelangen HRVs über rezeptorvermittelte Endocytose in das Zellinnere. Im Zuge dieser Transportroute werden Endosome angesäuert, was zur Konformationsänderung von aufgenommenen Viren führt. Bedingt durch diese Konformationsänderung gelingt es Viren, ihre RNA durch die Endosomenmembran in das Cytosol der infizierten Zelle zu transferieren. Daraus ergab sich mein Interesse an membrandestabilisierenden Fähigkeiten von viralen Proteinen sowie von ganzen Viren. Zusätzlich wurden auch grundlegende Experimente zum Transfer der viralen RNA selbst durch Membranen unter Zuhilfenahme von molcecular beacons durchgeführt. Molecular beacons sind fluoreszenzmarkierten Sonden, die nach erfolgter Bindung an eine korrespondierende Nukleotidsequenz ein Fluoreszenzsignal ergeben.

Human Rhinoviruses (HRVs) constitute the most frequent causative agents of common cold infections. Roughly 100 serotypes are differentiated, which are divided into two classes according to receptor specificity upon cell infection. Major group viruses bind to intercellular adhesion molecule 1 (ICAM-1), minor group viruses to members of the low-density-lipoprotein receptor (LDLR) family. We demonstrated previously that chip electrophoresis allows for analysis of fluorescence labeled virus and its bioaffinity reactions, for instance binding of minor group HRV2 to receptor fragments derived from the Very-Low-Density-Lipoprotein…

Subjects/Keywords: 35.29 Chromatographische Analyse, Elektrophorese; 42.32 Virologie; 35.30 Mikroanalyse, Spurenanalyse; Rhinovirus / Liposom / HRV2 / Bioanalyzer / Chip / Fluoreszenz / Elektrophorese / Molecular Beacon / Rezeptor / Infektion; rhinovirus / liposome / HRV2 / Bioanalyzer / chip / fluorescence / electrophoresis / molecular beacon / receptor / infection

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Weiss, V. (2009). Chip electrophoresis of human rhinovirus and receptor decorated liposomes as model membranes for the analysis of key steps in the viral infection pathway. (Thesis). University of Vienna. Retrieved from http://othes.univie.ac.at/7576/

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Weiss, Victor. “Chip electrophoresis of human rhinovirus and receptor decorated liposomes as model membranes for the analysis of key steps in the viral infection pathway.” 2009. Thesis, University of Vienna. Accessed January 18, 2021. http://othes.univie.ac.at/7576/.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Weiss, Victor. “Chip electrophoresis of human rhinovirus and receptor decorated liposomes as model membranes for the analysis of key steps in the viral infection pathway.” 2009. Web. 18 Jan 2021.

Vancouver:

Weiss V. Chip electrophoresis of human rhinovirus and receptor decorated liposomes as model membranes for the analysis of key steps in the viral infection pathway. [Internet] [Thesis]. University of Vienna; 2009. [cited 2021 Jan 18]. Available from: http://othes.univie.ac.at/7576/.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Weiss V. Chip electrophoresis of human rhinovirus and receptor decorated liposomes as model membranes for the analysis of key steps in the viral infection pathway. [Thesis]. University of Vienna; 2009. Available from: http://othes.univie.ac.at/7576/

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

26. Curnyn, Katie Elouise. Intracellular Detection of Viral Replication of Dengue Virus Serotype 1 Using a Molecular Beacon Fluorescent Probe.

Degree: Microbiology, 2016, University of California – Riverside

URL: http://www.escholarship.org/uc/item/1jq8f0v7

► ABSTRACT OF THE THESISIntracellular Detection of Viral Replication of Dengue Virus Serotype 1 using a Molecular Beacon Fluorescent ProbebyKatie Elouise CurnynMaster of Science, Graduate Program… (more)

Subjects/Keywords: Microbiology; Environmental science; Virology; Dengue; Fluorescence; Molecular Beacon; Probe; Virus detection

…11 Figure 4 Molecular Beacon Structure and Function… …x29;. Geneva: World 7 2.0 Molecular Beacon Structure and Function Current methods to… …of the assay and its specificity. A molecular beacon (MB) is a synthetic single… …Geneva: World Health Organization; 2009 11 Figure 4 Molecular Beacon Structure and Function… …placed in black cryogenic tube and frozen at -20℃. 15 3.5 Molecular Beacon and Complimentary…

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Curnyn, K. E. (2016). Intracellular Detection of Viral Replication of Dengue Virus Serotype 1 Using a Molecular Beacon Fluorescent Probe. (Thesis). University of California – Riverside. Retrieved from http://www.escholarship.org/uc/item/1jq8f0v7

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Curnyn, Katie Elouise. “Intracellular Detection of Viral Replication of Dengue Virus Serotype 1 Using a Molecular Beacon Fluorescent Probe.” 2016. Thesis, University of California – Riverside. Accessed January 18, 2021. http://www.escholarship.org/uc/item/1jq8f0v7.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Curnyn, Katie Elouise. “Intracellular Detection of Viral Replication of Dengue Virus Serotype 1 Using a Molecular Beacon Fluorescent Probe.” 2016. Web. 18 Jan 2021.

Vancouver:

Curnyn KE. Intracellular Detection of Viral Replication of Dengue Virus Serotype 1 Using a Molecular Beacon Fluorescent Probe. [Internet] [Thesis]. University of California – Riverside; 2016. [cited 2021 Jan 18]. Available from: http://www.escholarship.org/uc/item/1jq8f0v7.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Curnyn KE. Intracellular Detection of Viral Replication of Dengue Virus Serotype 1 Using a Molecular Beacon Fluorescent Probe. [Thesis]. University of California – Riverside; 2016. Available from: http://www.escholarship.org/uc/item/1jq8f0v7

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

27. Wood, Sharla Leann. Single molecule studies of rna-target interactions.

Degree: PhD, Chemistry, 2012, Wayne State University

URL: https://digitalcommons.wayne.edu/oa_dissertations/522

► We have used FRET, single molecule spectroscopy, and several other biophysical techniques to study how the folding of RNA allows it to perform its… (more)

Subjects/Keywords: aptamer, fluorescence, molecular beacon, riboswitch, RNA, Single molecule; Analytical Chemistry; Biochemistry

…82 4.2.3 The molecular beacon demonstrates a large conformational change upon target… …97 4.4.1 Molecular beacon purification and labeling… …the 2’OH of the molecular beacon RNA backbone �� .78 Figure 4.3 Native… …82 Figure 4.5 The molecular beacon demonstrates a large dynamic range ��.85 Figure 4.6… …Molecular beacon single molecule FRET setup �� 87 x Figure 4.7 Representative single…

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Wood, S. L. (2012). Single molecule studies of rna-target interactions. (Doctoral Dissertation). Wayne State University. Retrieved from https://digitalcommons.wayne.edu/oa_dissertations/522

Chicago Manual of Style (16^th Edition):

Wood, Sharla Leann. “Single molecule studies of rna-target interactions.” 2012. Doctoral Dissertation, Wayne State University. Accessed January 18, 2021. https://digitalcommons.wayne.edu/oa_dissertations/522.

MLA Handbook (7^th Edition):

Wood, Sharla Leann. “Single molecule studies of rna-target interactions.” 2012. Web. 18 Jan 2021.

Vancouver:

Wood SL. Single molecule studies of rna-target interactions. [Internet] [Doctoral dissertation]. Wayne State University; 2012. [cited 2021 Jan 18]. Available from: https://digitalcommons.wayne.edu/oa_dissertations/522.

Council of Science Editors:

Wood SL. Single molecule studies of rna-target interactions. [Doctoral Dissertation]. Wayne State University; 2012. Available from: https://digitalcommons.wayne.edu/oa_dissertations/522

28. Alsaiari, Shahad K. Novel Metal Clusters for Imaging Applications.

Degree: 2014, King Abdullah University of Science and Technology

URL: http://hdl.handle.net/10754/313850

► During the past few years, gold nanoparticles (AuNPs) have received considerable attention in many fields due to their optical properties, photothermal effect and biocompatibility. AuNPs,… (more)

Subjects/Keywords: Antioxidants; Gold Nanoclusters; Glutathione; Magnetotactic Bacteria; Gold Nanoclustersrods; Molecular Beacon DNA

…strains of MTB were loaded with gold-quenched molecular beacon (MB) DNA. MTB were used… …bromide) MTB Magnetotactic bacteria AMF Alternating magnetic field MB Molecular… …beacon 9 HSV-1 Herpes simplex virus-1 VLPs Virus-like particles siRNA Small interfering… …fluorophores) photo-bleaching. AuNRs were used as a contrast agent for molecular imaging using… …have considerable advantages over molecular dyes and fluorophores; First, their photophysical…

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Alsaiari, S. K. (2014). Novel Metal Clusters for Imaging Applications. (Thesis). King Abdullah University of Science and Technology. Retrieved from http://hdl.handle.net/10754/313850

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Alsaiari, Shahad K. “Novel Metal Clusters for Imaging Applications.” 2014. Thesis, King Abdullah University of Science and Technology. Accessed January 18, 2021. http://hdl.handle.net/10754/313850.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Alsaiari, Shahad K. “Novel Metal Clusters for Imaging Applications.” 2014. Web. 18 Jan 2021.

Vancouver:

Alsaiari SK. Novel Metal Clusters for Imaging Applications. [Internet] [Thesis]. King Abdullah University of Science and Technology; 2014. [cited 2021 Jan 18]. Available from: http://hdl.handle.net/10754/313850.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Alsaiari SK. Novel Metal Clusters for Imaging Applications. [Thesis]. King Abdullah University of Science and Technology; 2014. Available from: http://hdl.handle.net/10754/313850

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

29. Darko, Janice. Fluorescent Labeling of Antibiotic Resistant Bacteria Model DNA.

Degree: MS, 2018, Brigham Young University

URL: https://scholarsarchive.byu.edu/cgi/viewcontent.cgi?article=8600&context=etd

► Global threats to treatment of bacterial infections due to antibiotic resistance (AR) have been on the rise in recent years. Current diagnostic tests identify… (more)

Subjects/Keywords: antibiotic resistance; fluorescence labeling; oligreen dye; molecular beacon; Physical Sciences and Mathematics

…standard calibration curve was plotted. 2.3.2 Molecular Beacon Quantitation The fluorescence… …W.H. Designing a novel molecular beacon for surface-immobilized DNA hybridization studies. J… …oligreen dye (OG), molecular beacons (MB), and magnetic beads. 1.2 DNA… …molecular beacons, and solid supports such as magnetic beads have been employed over the years to… …how OG is used to label DNA. 1.2.2 Molecular Beacons MBs are short oligonucleotide, dual…

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APA (6^th Edition):

Darko, J. (2018). Fluorescent Labeling of Antibiotic Resistant Bacteria Model DNA. (Masters Thesis). Brigham Young University. Retrieved from https://scholarsarchive.byu.edu/cgi/viewcontent.cgi?article=8600&context=etd

Chicago Manual of Style (16^th Edition):

Darko, Janice. “Fluorescent Labeling of Antibiotic Resistant Bacteria Model DNA.” 2018. Masters Thesis, Brigham Young University. Accessed January 18, 2021. https://scholarsarchive.byu.edu/cgi/viewcontent.cgi?article=8600&context=etd.

MLA Handbook (7^th Edition):

Darko, Janice. “Fluorescent Labeling of Antibiotic Resistant Bacteria Model DNA.” 2018. Web. 18 Jan 2021.

Vancouver:

Darko J. Fluorescent Labeling of Antibiotic Resistant Bacteria Model DNA. [Internet] [Masters thesis]. Brigham Young University; 2018. [cited 2021 Jan 18]. Available from: https://scholarsarchive.byu.edu/cgi/viewcontent.cgi?article=8600&context=etd.

Council of Science Editors:

Darko J. Fluorescent Labeling of Antibiotic Resistant Bacteria Model DNA. [Masters Thesis]. Brigham Young University; 2018. Available from: https://scholarsarchive.byu.edu/cgi/viewcontent.cgi?article=8600&context=etd

30. Ντζιώρα, Φωτεινή. Ποσοτικοποίηση των στελεχών του ιού της ηπατίτιδας Β (HBV) με τις μεταλλαγές που προσδίδουν αντοχή στην αντιϊκή θεραπεία.

Degree: 2011, National and Kapodistrian University of Athens; Εθνικό και Καποδιστριακό Πανεπιστήμιο Αθηνών (ΕΚΠΑ)

URL: http://hdl.handle.net/10442/hedi/31781

►

BACKGROUND: Resistance has frequently been associated with the emergence of resistance-associated mutations and is a major problem when treating patients with hepatitis B virus (HBV)… (more)

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BACKGROUND: Resistance has frequently been associated with the emergence of resistance-associated mutations and is a major problem when treating patients with hepatitis B virus (HBV) infection. Conventional methods often fail to detect HBV viral subpopulations if they are represented in less than 20% of the total.AIM: Development of a novel method for the quantitative detection of HBV subpopulations with major resistance associated mutations (V173L, L180M, A181V, T184A, M204I/V and N236T).METHODS: Amplification Refractory Mutation System real-time PCR (ARMS rt-PCR) was combined with molecular beacon using the LightCycler system.RESULTS: ARMS rt-PCR with molecular beacon showed a sensitivity of 10 copies/reaction and analytical sensitivity 95% for mutant variant and 100% for wild-type variant. Discriminatory ability is 0.01%-0.25%. In HBV naïve patients viral populations with resistance associated mutations (V173L, L180M, A181V, T184A, M204I and N236T) were present in 38/60 (63.3%). In HBV treated patients who have shown resistance, viral populations with resistance associated mutations were present both as dominant and minor variants, probably affecting the patients’ resistance profile.CONCLUSIONS: Although the sensitivity, precision (inter-assay, intra-assay) and discriminatory ability of the ARMS rt-PCR with molecular beacon is similar to other ultra sensitive methods, such as ultra-deep sequencing, we show that the former can be applied in a cost-effective way for the detection of HBV subpopulations with major resistance associated mutations.

ΕΙΣΑΓΩΓΗ: Η ανάπτυξη αντοχής στην αντιϊκή θεραπεία έχει σχετισθεί με την εμφάνιση μεταλλαγών και αποτελεί ένα βασικό πρόβλημα στην αντιμετώπιση της λοίμωξης του ιού της ηπατίτιδας Β (HBV). Οι συμβατικές μέθοδοι αδυνατούν να ανιχνεύσουν ιϊκούς υποπληθυσμούς με τις μεταλλαγές αντοχής αν αντιπροσωπεύουν λιγότερο από το 20% του συνολικού πληθυσμού του ιού στο υπό εξέταση δείγμα. ΣΚΟΠΟΣ: Η δημιουργία μιας μεθόδου για την ποσοτικοποίηση των ιϊκών υποπληθυσμών του HBV με τις κυριότερες μεταλλαγές που προσδίδουν αντοχή στην αντιϊκή θεραπεία (V173L, L180M, A181V, T184A, M204I/V και N236T).ΥΛΙΚΟ-ΜΕΘΟΔΟΣ: Η μεθοδολογία του συστήματος αναστολής της γονιδιακής επέκτασης του μεταλλαγμένου τύπου με PCR πραγματικού χρόνου (amplification refractory mutation system, ARMS RT-PCR) συνδυάστηκε με την τεχνολογία του μοριακού φάρου (molecular beacon) χρησιμοποιώντας το σύστημα του LightCycler®. ΑΠΟΤΕΛΕΣΜΑΤΑ: Η μέθοδος ARMS rt-PCR με μοριακό φάρο έχει ευαισθησία 10 αντιγράφων ανά αντίδραση και αναλυτική ευαισθησία 95% για το μεταλλαγμένο τύπο και 100% για το φυσικό τύπο. Η διακριτική ικανότητα της μεθόδου είναι 0.01%-0.25%. Ιϊκοί υποπληθυσμοί με τις μεταλλαγές αντοχής του HBV V173L, L180M, A181V, T184A, M204I και N236T βρέθηκαν σε υψηλή συχνότητα (38/60, 63.3%), σε δείγματα ασθενών με ηπατίτιδα Β, που δεν είχαν λάβει ποτέ θεραπεία με νουκλεοσ(τ)ιδικά ανάλογα. Σε 41 δείγματα ασθενών με HBV λοίμωξη, που είχαν λάβει θεραπεία, εκτός από τις μεταλλαγές αντοχής με επικρατούντα ιϊκό υποπληθυσμό,…

Subjects/Keywords: Ηπατίτιδα B (HBV); Μεταλλαγές αντοχής; Ιϊκοί υποπληθυσμοί; Αντιική θεραπεία; Ποσοτική ανίχνευση; Πραγματικού χρόνου PCR; Μοριακός φάρος; Hepatitis B; Resistance mutations; Viral qusispecies; Antiviral treatment; Quantitative detection; Real time PCR; Molecular beacon

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Ντζιώρα, . �. (2011). Ποσοτικοποίηση των στελεχών του ιού της ηπατίτιδας Β (HBV) με τις μεταλλαγές που προσδίδουν αντοχή στην αντιϊκή θεραπεία. (Thesis). National and Kapodistrian University of Athens; Εθνικό και Καποδιστριακό Πανεπιστήμιο Αθηνών (ΕΚΠΑ). Retrieved from http://hdl.handle.net/10442/hedi/31781

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Ντζιώρα, Φωτεινή. “Ποσοτικοποίηση των στελεχών του ιού της ηπατίτιδας Β (HBV) με τις μεταλλαγές που προσδίδουν αντοχή στην αντιϊκή θεραπεία.” 2011. Thesis, National and Kapodistrian University of Athens; Εθνικό και Καποδιστριακό Πανεπιστήμιο Αθηνών (ΕΚΠΑ). Accessed January 18, 2021. http://hdl.handle.net/10442/hedi/31781.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Ντζιώρα, Φωτεινή. “Ποσοτικοποίηση των στελεχών του ιού της ηπατίτιδας Β (HBV) με τις μεταλλαγές που προσδίδουν αντοχή στην αντιϊκή θεραπεία.” 2011. Web. 18 Jan 2021.

Vancouver:

Ντζιώρα �. Ποσοτικοποίηση των στελεχών του ιού της ηπατίτιδας Β (HBV) με τις μεταλλαγές που προσδίδουν αντοχή στην αντιϊκή θεραπεία. [Internet] [Thesis]. National and Kapodistrian University of Athens; Εθνικό και Καποδιστριακό Πανεπιστήμιο Αθηνών (ΕΚΠΑ); 2011. [cited 2021 Jan 18]. Available from: http://hdl.handle.net/10442/hedi/31781.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Ντζιώρα �. Ποσοτικοποίηση των στελεχών του ιού της ηπατίτιδας Β (HBV) με τις μεταλλαγές που προσδίδουν αντοχή στην αντιϊκή θεραπεία. [Thesis]. National and Kapodistrian University of Athens; Εθνικό και Καποδιστριακό Πανεπιστήμιο Αθηνών (ΕΚΠΑ); 2011. Available from: http://hdl.handle.net/10442/hedi/31781

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

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		in
And / Or
		in

Open Access Theses and Dissertations