You searched for subject:(Model animal)
.
Showing records 1 – 30 of
703 total matches.
◁ [1] [2] [3] [4] [5] … [24] ▶

Wake Forest University
1.
Marquez-Lara, Alejandro J.
INFLAMMATION AND FRACTURE HEALING: A NEW PARADIGM.
Degree: 2019, Wake Forest University
URL: http://hdl.handle.net/10339/94326
► Inflammation plays a fundamental role in bone healing. This complex process is regulated by a myriad of cells and inflammatory mediators that create an adequate…
(more)
▼ Inflammation plays a fundamental role in bone healing. This complex process is regulated by a myriad of cells and inflammatory mediators that create an adequate environment for subsequent stages of the bone healing cascade. Despite this awareness, most of our understanding of the bone healing process derives from experimental animal models that fail to take into consideration the impact of surgical fixation on fracture healing. The ability to apply pre-clinical findings to clinical practice depends on the translational characteristics of a specific model. As such the first part of this body of work is focused on developing and describing a novel translational fracture model that accounts for surgical fixation as a separate and independent event in the fracture healing process.
Subjects/Keywords: Animal model
Record Details
Similar Records
Cite
Share »
Record Details
Similar Records
Cite
« Share





❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Marquez-Lara, A. J. (2019). INFLAMMATION AND FRACTURE HEALING: A NEW PARADIGM. (Thesis). Wake Forest University. Retrieved from http://hdl.handle.net/10339/94326
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Marquez-Lara, Alejandro J. “INFLAMMATION AND FRACTURE HEALING: A NEW PARADIGM.” 2019. Thesis, Wake Forest University. Accessed February 28, 2021.
http://hdl.handle.net/10339/94326.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Marquez-Lara, Alejandro J. “INFLAMMATION AND FRACTURE HEALING: A NEW PARADIGM.” 2019. Web. 28 Feb 2021.
Vancouver:
Marquez-Lara AJ. INFLAMMATION AND FRACTURE HEALING: A NEW PARADIGM. [Internet] [Thesis]. Wake Forest University; 2019. [cited 2021 Feb 28].
Available from: http://hdl.handle.net/10339/94326.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Marquez-Lara AJ. INFLAMMATION AND FRACTURE HEALING: A NEW PARADIGM. [Thesis]. Wake Forest University; 2019. Available from: http://hdl.handle.net/10339/94326
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Alberta
2.
Alrobaiea, Saad Mohammad.
A Novel Nude Mouse Model of Hypertrophic Scarring Using
Scratched Full-Thickness Human Skin Grafts.
Degree: MS, Department of Surgery, 2015, University of Alberta
URL: https://era.library.ualberta.ca/files/c4q77fr539
► Hypertrophic scar (HTS) is a dermal form of fibroproliferative disorder that develops following deep skin injury. This dermal fibrosis can cause deformities, functional disabilities, and…
(more)
▼ Hypertrophic scar (HTS) is a dermal form of
fibroproliferative disorder that develops following deep skin
injury. This dermal fibrosis can cause deformities, functional
disabilities, and aesthetic disfigurements. The pathophysiology of
HTS is not understood due in part, to the lack of an ideal animal
model. We hypothesize that human skin with dermal wounds of a depth
known to reproducibly cause HTS once grafted onto athymic nude mice
will develop a raised elevated scar similar to HTS seen in humans.
Our aim is to develop a representative animal model of human HTS
and to explore the cellular origin of the fibrosis either locally
derived from the deep dermal human fibroblasts or from the bone
marrow derived monocytes of the mouse. Thirty-six nude mice were
grafted with full thickness human skin with deep dermal scratch
wound before or 2 weeks after grafting. The scratch on the human
skin grafts was made using a specially designed jig creates a wound
> 0.6 mm in depth which has been demonstrated to be the
depth of injury in human skin volunteers beyond which HTS
consistently develops. Deep dermal wounds in the human skin grafts
transplanted on to the dorsal surface of athymic mice were
morphologically analyzed by digital photography. Mice were
euthanized at one, two, and three months postoperatively before
histological analysis of scar thickness, collagen synthesis and
fiber orientation, mast cells, macrophages, human leukocyte
antigen-ABC, and myofibroblasts was performed. Morphologic
persistence of human fibroblasts in the raised elevated scar was
documented up to one-year post engraftment using human anti-ABC
antibodies. The mice developed red, raised, and firm scars in the
scratched xenografts with more contraction, increased recruitment
of macrophages and myofibroblasts as compared to the xenografts
without deep dermal scratch wound. Scar thickness and collagen
bundle orientation and morphology were resembled that seen in human
HTS in the deep dermal wounds within the engrafted human skin on
the dorsum of the mice. The fibrotic scars in the wounded human
skin were morphologically and histologically similar to human HTS
and human skin epithelial cells and fibroblasts persisted in the
remodelling tissues for at least one year post-engraftment. Thus,
deep dermal injury in human skin retains its profibrotic nature
after transplantation, affording a novel model for the assessment
of therapies for the treatment of human fibroproliferative
disorders of the skin.
Subjects/Keywords: Hypertrophic Scars; Animal model; Scars
Record Details
Similar Records
Cite
Share »
Record Details
Similar Records
Cite
« Share





❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Alrobaiea, S. M. (2015). A Novel Nude Mouse Model of Hypertrophic Scarring Using
Scratched Full-Thickness Human Skin Grafts. (Masters Thesis). University of Alberta. Retrieved from https://era.library.ualberta.ca/files/c4q77fr539
Chicago Manual of Style (16th Edition):
Alrobaiea, Saad Mohammad. “A Novel Nude Mouse Model of Hypertrophic Scarring Using
Scratched Full-Thickness Human Skin Grafts.” 2015. Masters Thesis, University of Alberta. Accessed February 28, 2021.
https://era.library.ualberta.ca/files/c4q77fr539.
MLA Handbook (7th Edition):
Alrobaiea, Saad Mohammad. “A Novel Nude Mouse Model of Hypertrophic Scarring Using
Scratched Full-Thickness Human Skin Grafts.” 2015. Web. 28 Feb 2021.
Vancouver:
Alrobaiea SM. A Novel Nude Mouse Model of Hypertrophic Scarring Using
Scratched Full-Thickness Human Skin Grafts. [Internet] [Masters thesis]. University of Alberta; 2015. [cited 2021 Feb 28].
Available from: https://era.library.ualberta.ca/files/c4q77fr539.
Council of Science Editors:
Alrobaiea SM. A Novel Nude Mouse Model of Hypertrophic Scarring Using
Scratched Full-Thickness Human Skin Grafts. [Masters Thesis]. University of Alberta; 2015. Available from: https://era.library.ualberta.ca/files/c4q77fr539
3.
Monga, Jitender.
Antiproliferative and chemopreventive potential of acacia
catechu willd against multiorgan carcinoma.
Degree: Pharmaceutical Sciences, 2013, Jaypee University of Information Technology, Solan
URL: http://shodhganga.inflibnet.ac.in/handle/10603/14082
► Cancer or the malignancies of the cells has been considered as one of the most dreadly disease. Tissue invasiveness and metastatic spread of cancer cells…
(more)
▼ Cancer or the malignancies of the cells has been
considered as one of the most dreadly disease. Tissue invasiveness
and metastatic spread of cancer cells are liable for most of the
morbidity and mortality allied with the disease. Agents inhibit the
proliferation of cancer cells by increasing apoptosis may provide a
useful mechanistic approach to both cancer chemoprevention and
chemotherapy. However, adverse effects and resistance of many of
the anticancer agents are serious problems. In recent years, number
of natural products has gained tremendous attention because of
their ability to induce apoptosis in cancer cells. Thus, there is
sprouting interest in the use of plant based compounds to develop
safe and efficient therapeutic agents for information for their
possible application in cancer therapy. Flavonoids are a diverse
group of low molecular weight polyphenolic compounds that are
widely distributed in nature and renewed interest has been observed
in recent years in the novel and multiple activities of flavonoids.
Acacia catechu Willd. (Fabaceae) also known as ‘Khadira’ has been
used extensively as traditional medicine in India/Asia over a long
period of time. (+)-Catechin [3',4',5,7-tetrahydroxyflavan-3-ol] is
the most abundant polyphenolic flavonoid in the Acacia catechu
heartwood.
References p. 206-226
Advisors/Committee Members: Sharma, Manu.
Subjects/Keywords: Acacia Catechu; Animal Model; Carcinoma
Record Details
Similar Records
Cite
Share »
Record Details
Similar Records
Cite
« Share





❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Monga, J. (2013). Antiproliferative and chemopreventive potential of acacia
catechu willd against multiorgan carcinoma. (Thesis). Jaypee University of Information Technology, Solan. Retrieved from http://shodhganga.inflibnet.ac.in/handle/10603/14082
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Monga, Jitender. “Antiproliferative and chemopreventive potential of acacia
catechu willd against multiorgan carcinoma.” 2013. Thesis, Jaypee University of Information Technology, Solan. Accessed February 28, 2021.
http://shodhganga.inflibnet.ac.in/handle/10603/14082.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Monga, Jitender. “Antiproliferative and chemopreventive potential of acacia
catechu willd against multiorgan carcinoma.” 2013. Web. 28 Feb 2021.
Vancouver:
Monga J. Antiproliferative and chemopreventive potential of acacia
catechu willd against multiorgan carcinoma. [Internet] [Thesis]. Jaypee University of Information Technology, Solan; 2013. [cited 2021 Feb 28].
Available from: http://shodhganga.inflibnet.ac.in/handle/10603/14082.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Monga J. Antiproliferative and chemopreventive potential of acacia
catechu willd against multiorgan carcinoma. [Thesis]. Jaypee University of Information Technology, Solan; 2013. Available from: http://shodhganga.inflibnet.ac.in/handle/10603/14082
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Otago
4.
Grant, Mark Wayne.
Anatomical and physiological characterization of a novel porcine model for varicose veins
.
Degree: 2012, University of Otago
URL: http://hdl.handle.net/10523/2235
► Varicose veins constitute a significant financial and social burden. Despite being well documented since Hippocrates, there is still a lack of understanding concerning both the…
(more)
▼ Varicose veins constitute a significant financial and social burden. Despite being well documented since Hippocrates, there is still a lack of understanding concerning both the underlying pathogenesis and the rationale of treatments of varicose veins. The management of varicose veins is marked by confusion and debate, which could benefit from well-designed experiments in a suitable
animal model. Especially as the current available therapies for the treatment of varicose veins is limited by their high recurrence rates.
While studying neointimal hyperplasia in arterial ends of common femoral AVF created in pig models we, at the Department of Surgery in Dunedin Hospital, observed that the pigs also developed progressive enlargement and tortuosity of the superficial veins over the medial aspect the thigh and groin. These changes in superficial veins developed into what appeared to be an extensive network of varicose veins. This novel finding has not been described to the same extent in other
animal models of venous disease. Consequently, the aim of this thesis is to further characterize a porcine
model of varicose veins.
Eleven female domesticated large white Duroc cross pigs, aged 13-14 weeks and weights 25.1- 35 kg, were used in this research project (control pigs [n=2], surgery animals [n=9]). The first
animal was used to assist in the characterisation the normal anatomy of pigs’ hind limb, the nominal saphenous vein and its relations and the sapheno- femoral junction. Each of the remaining animals had a right common femoral side to side arteriovenous fistula (AVF) fashioned by a vascular surgeon. Post-operatively the animals were assessed to document of macroscopic changes of venous vasculature, characterise the physiological changes that occur within the superficial venous system and to document any histological changes in the vessel wall.
Venous hypertension was demonstrated within the superficial varicosities, 23±11.4 mmHg at the mid point of the study, and 20±8.3 mmHg at termination, while in the control it was 4.5± 3.5 mmHg. In all but one pig with an AVF there was variation in pressure, associated with the cardiac cycle. The flow velocities also demonstrated a degree of pulsatility.
Overall there was both an intra-vessel and inter-
animal heterogeneity to the vessel walls changes seen in the veins sampled. Finding which were consistently seen included: variable neointimal formation, medial hypertrophy, fragmentation of the medial and adventitial elastic tissue, and medial and mural atrophy. Areas of greatest intimal thickness were associated with disruption of the internal elastic lamina. A variety of valvular defects were also observed with the specimens including bilateral and unilateral elongation of cusps, and valve cusp tearing.
In conclusion we describe a stable and chronic porcine AVF
model of venous insufficiency, which is simple to create and closely replicates the human condition of venous insufficiency and superficial varicose veins. The formation of an AVF between the femoral vessels…
Advisors/Committee Members: Jones, Gregory (advisor).
Subjects/Keywords: varicose;
animal;
veins;
model
Record Details
Similar Records
Cite
Share »
Record Details
Similar Records
Cite
« Share





❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Grant, M. W. (2012). Anatomical and physiological characterization of a novel porcine model for varicose veins
. (Masters Thesis). University of Otago. Retrieved from http://hdl.handle.net/10523/2235
Chicago Manual of Style (16th Edition):
Grant, Mark Wayne. “Anatomical and physiological characterization of a novel porcine model for varicose veins
.” 2012. Masters Thesis, University of Otago. Accessed February 28, 2021.
http://hdl.handle.net/10523/2235.
MLA Handbook (7th Edition):
Grant, Mark Wayne. “Anatomical and physiological characterization of a novel porcine model for varicose veins
.” 2012. Web. 28 Feb 2021.
Vancouver:
Grant MW. Anatomical and physiological characterization of a novel porcine model for varicose veins
. [Internet] [Masters thesis]. University of Otago; 2012. [cited 2021 Feb 28].
Available from: http://hdl.handle.net/10523/2235.
Council of Science Editors:
Grant MW. Anatomical and physiological characterization of a novel porcine model for varicose veins
. [Masters Thesis]. University of Otago; 2012. Available from: http://hdl.handle.net/10523/2235

University of Melbourne
5.
LIU, HSIN-HUA.
A novel rat model of ocular hypertension induced by a circumlimbal suture.
Degree: 2014, University of Melbourne
URL: http://hdl.handle.net/11343/51340
► The aim of the thesis is to develop a rat model of non-inflammatory, chronic ocular hypertension that produces glaucomatous optic neuropathy. Oculo-pression with circumlimbal suture…
(more)
▼ The aim of the thesis is to develop a rat model of non-inflammatory, chronic ocular hypertension that produces glaucomatous optic neuropathy. Oculo-pression with circumlimbal suture produces a mild chronic ocular hypertension for over 15 weeks that gives rise to a preferential ganglion cell dysfunction, nerve fibre layer thinning and ganglion cell loss. These changes can be reversed by cutting the suture to produce IOP lowering. The model can be used to explore factors that lead to irreversible changes in glaucoma.
Subjects/Keywords: animal model of glaucoma
Record Details
Similar Records
Cite
Share »
Record Details
Similar Records
Cite
« Share





❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
LIU, H. (2014). A novel rat model of ocular hypertension induced by a circumlimbal suture. (Doctoral Dissertation). University of Melbourne. Retrieved from http://hdl.handle.net/11343/51340
Chicago Manual of Style (16th Edition):
LIU, HSIN-HUA. “A novel rat model of ocular hypertension induced by a circumlimbal suture.” 2014. Doctoral Dissertation, University of Melbourne. Accessed February 28, 2021.
http://hdl.handle.net/11343/51340.
MLA Handbook (7th Edition):
LIU, HSIN-HUA. “A novel rat model of ocular hypertension induced by a circumlimbal suture.” 2014. Web. 28 Feb 2021.
Vancouver:
LIU H. A novel rat model of ocular hypertension induced by a circumlimbal suture. [Internet] [Doctoral dissertation]. University of Melbourne; 2014. [cited 2021 Feb 28].
Available from: http://hdl.handle.net/11343/51340.
Council of Science Editors:
LIU H. A novel rat model of ocular hypertension induced by a circumlimbal suture. [Doctoral Dissertation]. University of Melbourne; 2014. Available from: http://hdl.handle.net/11343/51340

University of Southern California
6.
Liu, Ying.
Insights from mouse models on the origin of ovarian cancer
and its predisposing mechanisms.
Degree: PhD, Pathobiology, 2014, University of Southern California
URL: http://digitallibrary.usc.edu/cdm/compoundobject/collection/p15799coll3/id/247625/rec/3514
► Ovarian cancers are the leading cause of death from gynecological cancer in the U.S. and more than 70% of the patients are diagnosed with disseminated…
(more)
▼ Ovarian cancers are the leading cause of death from
gynecological cancer in the U.S. and more than 70% of the patients
are diagnosed with disseminated disease. Thus, the ability to
detect ovarian cancer precursor lesions before they develop into
fully mature cancers would have a profound impact on the morbidity
and mortality of the disease. However, the exact site of origin of
ovarian cancer is not fully understood. The purpose of my thesis is
to develop heritable mouse models for epithelial ovarian tumors to
answer key questions regarding ovarian cancer origin and address
the underlying predisposing mechanisms. ❧ Dr. Dubeau has argued for
many years, that the currently favoured hypothesis that tumors
lesions that are currently classified ovarian epithelial tumors do
not arise from the coelomic epithelium that covers the ovary, but
from derivatives of the müllerian ducts. In order to test our
hypothesis, we generated transgenic constructs where the Müllerian
inhibiting substance type 2 receptor (Mis2r) promoter drives
expression of either β-galactosidase (Mis2r-β–Gal) or Cre
recombinase (Mis2r-Cre), we found no evidence of an embryological
link between the müllerian ducts and coelomic epithelium, however,
a segment of the renal tubules specific for the female gender might
suggest an embryological link between the müllerian ducts and renal
development. This is important not only to a development point of
view, but also helped us understand histogenesis of clear cell
ovarian carcinomas. ❧ We also created a mouse
model by
superimposing p53 knockout targeted specifically to müllerian tract
on the Brca1 mutation already present in Fshr-Cre; Brca1 flox/flox
mice. Tumors possibly from mammary glands were observed in 60% of
mice with double knockout, and they were epithelial in nature. This
model would be a useful tool to study the role of loss of function
of p53 and Brca1 in cancers relevant to ovary and mammary glands in
origin, which will further augment our understanding on human
BRCA1-mutated related cancer. ❧ The gene profiling changes between
wide type and mutant mice with Brca1 inactivation in ovarian
granulosa cells showed the gene family that was most frequently
influenced was that of olfactory receptor. We validated the
presence of olfactory receptors in mouse and human granulosa cells
and further hypothesized those olfactory receptors may play a role
in signal transduction in granulosa cells and contribute to ovarian
tumor predisposition by regulating mice estrous cycle. ❧ Our
previous work has been using homozygous Brca1 mutation in ovarian
granulosa cells to maximize the effects of Brca1 inactivation, we
also want to test our hypothesis that the effects of a heterozygous
mutation is similar due to the gene dosage effect, such as present
in human BRCA1 mutation carriers.
Advisors/Committee Members: Dubeau, Louis (Committee Chair), Taylor, Clive R. (Committee Member), Chuong, Cheng-Ming (Committee Member), Maxson, Robert E., Jr. (Committee Member).
Subjects/Keywords: ovarian cancer; animal model
Record Details
Similar Records
Cite
Share »
Record Details
Similar Records
Cite
« Share





❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Liu, Y. (2014). Insights from mouse models on the origin of ovarian cancer
and its predisposing mechanisms. (Doctoral Dissertation). University of Southern California. Retrieved from http://digitallibrary.usc.edu/cdm/compoundobject/collection/p15799coll3/id/247625/rec/3514
Chicago Manual of Style (16th Edition):
Liu, Ying. “Insights from mouse models on the origin of ovarian cancer
and its predisposing mechanisms.” 2014. Doctoral Dissertation, University of Southern California. Accessed February 28, 2021.
http://digitallibrary.usc.edu/cdm/compoundobject/collection/p15799coll3/id/247625/rec/3514.
MLA Handbook (7th Edition):
Liu, Ying. “Insights from mouse models on the origin of ovarian cancer
and its predisposing mechanisms.” 2014. Web. 28 Feb 2021.
Vancouver:
Liu Y. Insights from mouse models on the origin of ovarian cancer
and its predisposing mechanisms. [Internet] [Doctoral dissertation]. University of Southern California; 2014. [cited 2021 Feb 28].
Available from: http://digitallibrary.usc.edu/cdm/compoundobject/collection/p15799coll3/id/247625/rec/3514.
Council of Science Editors:
Liu Y. Insights from mouse models on the origin of ovarian cancer
and its predisposing mechanisms. [Doctoral Dissertation]. University of Southern California; 2014. Available from: http://digitallibrary.usc.edu/cdm/compoundobject/collection/p15799coll3/id/247625/rec/3514

University of New South Wales
7.
Rodriguez Paris, Valentina.
Impact of hyperandrogenism and diet on the development of polycystic ovary syndrome.
Degree: Women's & Children's Health, 2020, University of New South Wales
URL: http://handle.unsw.edu.au/1959.4/69717
;
https://unsworks.unsw.edu.au/fapi/datastream/unsworks:71270/SOURCE02?view=true
► Polycystic ovary syndrome (PCOS) is a heterogeneous disorder featuring reproductive, endocrine and metabolic abnormalities. Hyperandrogenism is a defining characteristic of PCOS and evidence supports a…
(more)
▼ Polycystic ovary syndrome (PCOS) is a heterogeneous disorder featuring reproductive, endocrine and metabolic abnormalities. Hyperandrogenism is a defining characteristic of PCOS and evidence supports a role for androgen driven actions in the development of PCOS. The aetiology of PCOS is poorly understood and current management is symptom based. Therefore, defining the ontogeny of PCOS traits and the factors impacting their development, is important for developing early PCOS detection markers and new treatment strategies. The aims of this research were to determine the temporal pattern of development of PCOS features in a hyperandrogenic environment, define the impact of dietary macronutrient balance on hyperandrogenic PCOS traits and evaluate the impact of diet and a hyperandrogenic PCOS pathology on the gut microbiome using a mouse
model. The first study characterised the temporal pattern of development of PCOS features after excess androgen exposure. Findings identified that acyclicity, anovulation and increased body weight are early predictors of developing PCOS. The second study utilized the geometric framework for nutrition and reports the first systematic analysis of dietary protein, carbohydrate and fat on the evolution of reproductive and metabolic PCOS traits in a PCOS mouse
model. Results revealed that reproductive PCOS features of acyclicity and anovulation were ameliorated on low protein, medium carbohydrate and fat consumption. In contrast, diet had minimal effect on PCOS metabolic features. Specifically, PCOS mice were significantly heavier compared to controls due to an increased sensitivity to caloric intake, implying differences in post-ingestive responses to diet. Therefore, the third study investigated the interplay between diet and PCOS pathology on gut microbiota and revealed that diet exerted a stronger influence over gut microbial composition than PCOS. However, a PCOS environment did decrease levels of an obesity associated Bacteroides species. Overall, findings provide insights into the sequence of PCOS trait development, the impact of diet on PCOS features, and the interplay between diet and PCOS pathology on the gut microbiome, and support the addition of body weight criteria to the early diagnosis of PCOS and future research into the development of evidence-based nutritional and microbiome-mediated strategies for the treatment of PCOS.
Advisors/Committee Members: Walters, Kirsty, Women's & Children's Health, Faculty of Medicine, UNSW, Gilchrist, Robert, Women's & Children's Health, Faculty of Medicine, UNSW, Bertoldo, Michael, Women's & Children's Health, Faculty of Medicine, UNSW.
Subjects/Keywords: Diet; PCOS; Hyperandrogenism; Animal model
Record Details
Similar Records
Cite
Share »
Record Details
Similar Records
Cite
« Share





❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Rodriguez Paris, V. (2020). Impact of hyperandrogenism and diet on the development of polycystic ovary syndrome. (Doctoral Dissertation). University of New South Wales. Retrieved from http://handle.unsw.edu.au/1959.4/69717 ; https://unsworks.unsw.edu.au/fapi/datastream/unsworks:71270/SOURCE02?view=true
Chicago Manual of Style (16th Edition):
Rodriguez Paris, Valentina. “Impact of hyperandrogenism and diet on the development of polycystic ovary syndrome.” 2020. Doctoral Dissertation, University of New South Wales. Accessed February 28, 2021.
http://handle.unsw.edu.au/1959.4/69717 ; https://unsworks.unsw.edu.au/fapi/datastream/unsworks:71270/SOURCE02?view=true.
MLA Handbook (7th Edition):
Rodriguez Paris, Valentina. “Impact of hyperandrogenism and diet on the development of polycystic ovary syndrome.” 2020. Web. 28 Feb 2021.
Vancouver:
Rodriguez Paris V. Impact of hyperandrogenism and diet on the development of polycystic ovary syndrome. [Internet] [Doctoral dissertation]. University of New South Wales; 2020. [cited 2021 Feb 28].
Available from: http://handle.unsw.edu.au/1959.4/69717 ; https://unsworks.unsw.edu.au/fapi/datastream/unsworks:71270/SOURCE02?view=true.
Council of Science Editors:
Rodriguez Paris V. Impact of hyperandrogenism and diet on the development of polycystic ovary syndrome. [Doctoral Dissertation]. University of New South Wales; 2020. Available from: http://handle.unsw.edu.au/1959.4/69717 ; https://unsworks.unsw.edu.au/fapi/datastream/unsworks:71270/SOURCE02?view=true
8.
鮫島, 直樹.
The values of wall shear stress, turbulence kinetic energy and blood pressure gradient are associated with atherosclerotic plaque erosion in rabbits : 動脈硬化巣におけるびらん性傷害に関与する血行力学的因子の検討 : 家兎動脈硬化モデルでのコンピューターシュミレーション解析.
Degree: 博士(医学), 2014, University of Miyazaki / 宮崎大学
URL: http://hdl.handle.net/10458/4966
► 以下に掲載:Journal of Atherosclerosis and Thrombosis. 2014, 21, 8, p.831-838, doi: 10.5551/jat.23093.
Aim: To clarify the contribution of hemodynamic factors to the onset of plaque erosion…
(more)
▼ 以下に掲載:Journal of Atherosclerosis and Thrombosis. 2014, 21, 8, p.831-838, doi: 10.5551/jat.23093.
Aim: To clarify the contribution of hemodynamic factors to the onset of plaque erosion in smooth muscle cell (SMC)-rich atherosclerotic plaque.###Methods: We developed a rabbit model of SMC-rich atherosclerotic plaque with various degree of stenosis induced by incomplete ligation and generated three-dimensional models of five rabbit femoral arteries based on 130-162 serial histological cross-sections at 100-μm intervals per artery. We performed a computational blood flow simulation using the Reynolds-averaged Navier-Stokes model and calculated the wall shear stress (WSS), turbulence kinetic energy (TKE), blood pressure (BP) and blood pressure gradients (BPG) in eight sections (the inlet, the stenotic portion and areas 1, 2 and 5mm from the stenotic portion) in each rabbit. We also investigated whether the magnitude of WSS or TKE was related to the presence or absence of erosive injury by evaluating six points (the locally highest, median and lowest of WSS or TKE) in each section.###Results: The magnitudes of WSS, TKE and BPG, but not BP, correlated significantly with the extent of histologically-defined plaque erosion (WSS, r=0.55, p<0.001; TKE, r=0.53, p<0.001; BPG, r=0.61, p<0.0001, n=40). The values for WSS and TKE were significantly larger at sites with, compared to without, erosive injury (n=107 and n=119 points, respectively; both p<0.0001).###Conclusions: These results suggest that increased values of WSS, TKE and BPG considerably contribute to the onset of plaque erosion.
本論文の著作権は日本動脈硬化学会が保持しています。
Subjects/Keywords: Plaque erosion; Animal model; Computational fluid dynamics
Record Details
Similar Records
Cite
Share »
Record Details
Similar Records
Cite
« Share





❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
鮫島, . (2014). The values of wall shear stress, turbulence kinetic energy and blood pressure gradient are associated with atherosclerotic plaque erosion in rabbits : 動脈硬化巣におけるびらん性傷害に関与する血行力学的因子の検討 : 家兎動脈硬化モデルでのコンピューターシュミレーション解析. (Thesis). University of Miyazaki / 宮崎大学. Retrieved from http://hdl.handle.net/10458/4966
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
鮫島, 直樹. “The values of wall shear stress, turbulence kinetic energy and blood pressure gradient are associated with atherosclerotic plaque erosion in rabbits : 動脈硬化巣におけるびらん性傷害に関与する血行力学的因子の検討 : 家兎動脈硬化モデルでのコンピューターシュミレーション解析.” 2014. Thesis, University of Miyazaki / 宮崎大学. Accessed February 28, 2021.
http://hdl.handle.net/10458/4966.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
鮫島, 直樹. “The values of wall shear stress, turbulence kinetic energy and blood pressure gradient are associated with atherosclerotic plaque erosion in rabbits : 動脈硬化巣におけるびらん性傷害に関与する血行力学的因子の検討 : 家兎動脈硬化モデルでのコンピューターシュミレーション解析.” 2014. Web. 28 Feb 2021.
Vancouver:
鮫島 . The values of wall shear stress, turbulence kinetic energy and blood pressure gradient are associated with atherosclerotic plaque erosion in rabbits : 動脈硬化巣におけるびらん性傷害に関与する血行力学的因子の検討 : 家兎動脈硬化モデルでのコンピューターシュミレーション解析. [Internet] [Thesis]. University of Miyazaki / 宮崎大学; 2014. [cited 2021 Feb 28].
Available from: http://hdl.handle.net/10458/4966.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
鮫島 . The values of wall shear stress, turbulence kinetic energy and blood pressure gradient are associated with atherosclerotic plaque erosion in rabbits : 動脈硬化巣におけるびらん性傷害に関与する血行力学的因子の検討 : 家兎動脈硬化モデルでのコンピューターシュミレーション解析. [Thesis]. University of Miyazaki / 宮崎大学; 2014. Available from: http://hdl.handle.net/10458/4966
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Alberta
9.
Hong, Mandy.
The Effects of Docosahexaenoic Acid (DHA) Dietary
Supplementation in a Mouse Model of Stargardt-like Macular
Dystrophy (STGD3).
Degree: MS, Centre for Neuroscience, 2013, University of Alberta
URL: https://era.library.ualberta.ca/files/h702q689j
► Underlying mechanisms of how docosahexaenoic acid (DHA) might impact the progression of macular degeneration are unknown. We relied on the ELOVL4 mouse model of juvenile…
(more)
▼ Underlying mechanisms of how docosahexaenoic acid
(DHA) might impact the progression of macular degeneration are
unknown. We relied on the ELOVL4 mouse model of juvenile macular
degeneration, STGD3, to test the hypothesis that antenatal DHA
dietary supplementation (2% w/w of total fatty acid) would slow
down the progression of retinal degeneration as evidenced by
preserved function, anatomy, and DHA levels. DHA+, DHA-, and chow
diets commenced antenatally. Retina function was assessed by
electroretinogram at 1 and 3 months, while anatomical integrity and
fatty acid profiles were assessed with cross-sectional staining and
UPLC/MS/MS, respectively, at 3 months. Results showed a negative
ELOVL4 genotype effect on rod function, photoreceptor numbers, and
DHA/AA levels. Surprisingly, in transgenic retinas DHA
supplementation resulted in lower DHA levels compared to
non-supplemented. Although beneficial effects of DHA have been
reported, antenatal and 3 month postnatal supplementation is not
sufficient to elicit these effects in mice with
STGD3.
Subjects/Keywords: Docasahexaenoic Acid (DHA); Animal Model; Retinal Degeneration
Record Details
Similar Records
Cite
Share »
Record Details
Similar Records
Cite
« Share





❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Hong, M. (2013). The Effects of Docosahexaenoic Acid (DHA) Dietary
Supplementation in a Mouse Model of Stargardt-like Macular
Dystrophy (STGD3). (Masters Thesis). University of Alberta. Retrieved from https://era.library.ualberta.ca/files/h702q689j
Chicago Manual of Style (16th Edition):
Hong, Mandy. “The Effects of Docosahexaenoic Acid (DHA) Dietary
Supplementation in a Mouse Model of Stargardt-like Macular
Dystrophy (STGD3).” 2013. Masters Thesis, University of Alberta. Accessed February 28, 2021.
https://era.library.ualberta.ca/files/h702q689j.
MLA Handbook (7th Edition):
Hong, Mandy. “The Effects of Docosahexaenoic Acid (DHA) Dietary
Supplementation in a Mouse Model of Stargardt-like Macular
Dystrophy (STGD3).” 2013. Web. 28 Feb 2021.
Vancouver:
Hong M. The Effects of Docosahexaenoic Acid (DHA) Dietary
Supplementation in a Mouse Model of Stargardt-like Macular
Dystrophy (STGD3). [Internet] [Masters thesis]. University of Alberta; 2013. [cited 2021 Feb 28].
Available from: https://era.library.ualberta.ca/files/h702q689j.
Council of Science Editors:
Hong M. The Effects of Docosahexaenoic Acid (DHA) Dietary
Supplementation in a Mouse Model of Stargardt-like Macular
Dystrophy (STGD3). [Masters Thesis]. University of Alberta; 2013. Available from: https://era.library.ualberta.ca/files/h702q689j

Vanderbilt University
10.
Mergy, Marc Andrew.
Generation and Characterization of the First Construct-Valid Model of ADHD, the DAT Val559 Knock-In Mouse.
Degree: PhD, Neuroscience, 2013, Vanderbilt University
URL: http://hdl.handle.net/1803/14625
► Attention-deficit/hyperactivity disorder (ADHD) is the most commonly diagnosed childhood neuropsychiatric disorder. More than twenty years of genetic, behavioral, and pharmacological research support the hypothesis that…
(more)
▼ Attention-deficit/hyperactivity disorder (ADHD) is the most commonly diagnosed childhood neuropsychiatric disorder. More than twenty years of genetic, behavioral, and pharmacological research support the hypothesis that compromised brain dopamine (DA) signaling impacts risk for the disorder. To date, however, evidence for this idea is indirect, and we therefore lack a construct-valid
animal model for ADHD. To gain direct evidence for changes in DA-regulatory molecules in ADHD, we screened subjects with a DSM-IV diagnosis of the disorder and identified multiple, rare DA transporter (DAT, SLC6A3) coding variants. One of these variants, DAT Val559, induces anomalous, non-vesicular DA release in transfected cells, and efflux that can be blocked by the most common ADHD medications, D-amphetamine (AMPH) and methylphenidate. To pursue the significance of these findings in vivo, we engineered DAT Val559 knock-in mice and, as with heterologous expression studies, found the variant to support expression of wild-type (WT) levels of striatal DAT and tyrosine hydroxylase (TH) protein, as well as DA and DA metabolite levels. However, DAT Val559 mice exhibited a novel, conditional hyperactivity phenotype, “darting”, as well as an impaired sensitivity to the locomotor-activating properties of AMPH. In vivo microdialysis studies in the striatum of DAT Val559 animals demonstrated a pronounced elevation of basal, extracellular DA levels along with a significantly blunted efflux of DA release evoked by locally infused AMPH. Together, our studies confirm a functional impact on extracellular DA homeostasis of the DAT Val559 variant in vivo and establish the first construct-valid
animal model of ADHD.
Advisors/Committee Members: Hassane Mchaourab (committee member), Aurelio Galli (committee member), Eric Delpire (Committee Chair), Randy Blakely (Committee Chair).
Subjects/Keywords: transporter; dopamine; ADHD; transgenic; mouse; animal model
Record Details
Similar Records
Cite
Share »
Record Details
Similar Records
Cite
« Share





❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Mergy, M. A. (2013). Generation and Characterization of the First Construct-Valid Model of ADHD, the DAT Val559 Knock-In Mouse. (Doctoral Dissertation). Vanderbilt University. Retrieved from http://hdl.handle.net/1803/14625
Chicago Manual of Style (16th Edition):
Mergy, Marc Andrew. “Generation and Characterization of the First Construct-Valid Model of ADHD, the DAT Val559 Knock-In Mouse.” 2013. Doctoral Dissertation, Vanderbilt University. Accessed February 28, 2021.
http://hdl.handle.net/1803/14625.
MLA Handbook (7th Edition):
Mergy, Marc Andrew. “Generation and Characterization of the First Construct-Valid Model of ADHD, the DAT Val559 Knock-In Mouse.” 2013. Web. 28 Feb 2021.
Vancouver:
Mergy MA. Generation and Characterization of the First Construct-Valid Model of ADHD, the DAT Val559 Knock-In Mouse. [Internet] [Doctoral dissertation]. Vanderbilt University; 2013. [cited 2021 Feb 28].
Available from: http://hdl.handle.net/1803/14625.
Council of Science Editors:
Mergy MA. Generation and Characterization of the First Construct-Valid Model of ADHD, the DAT Val559 Knock-In Mouse. [Doctoral Dissertation]. Vanderbilt University; 2013. Available from: http://hdl.handle.net/1803/14625

McMaster University
11.
Nicolini, Chiara.
DEFECTIVE TrkB SIGNALING PATHWAYS IN IDIOPATHIC AUTISM.
Degree: PhD, 2016, McMaster University
URL: http://hdl.handle.net/11375/20034
► Autism is a neurodevelopmental disorder characterized by impairments in social communication and interaction and by repetitive patterns of behaviour, interests and activities. It is perhaps…
(more)
▼ Autism is a neurodevelopmental disorder characterized by impairments in social communication and interaction and by repetitive patterns of behaviour, interests and activities. It is perhaps the most common and handicapping neurological disorder of childhood and as such represents a significant public health problem and a huge burden for education and social service systems. Currently there is no diagnostic test or cure available for autism and the molecular mechanisms underlying autistic behaviour remain to be elucidated. Mutations in genes linked to autism adversely affect molecules involved in synapse development and plasticity including brain-derived neurotrophic factor receptor (TrkB) and its downstream effector mammalian target of rapamycin (mTOR), which is increased in several forms of syndromic autism.
Here, we investigated whether TrkB, mTOR and their signaling pathways are disrupted in postmortem brain tissue from subjects with idiopathic autism, that is, cases of autism without a known genetic cause and thought to be of environmental/epigenetic origin. We next further examined the contribution of defective TrkB signaling to autistic behaviour in mice exposed to the histone deacetylase inhibitor valproic acid (VPA), a well-established model of environmental/epigenetic origin of autism.
We found that TrkB signaling pathways were reduced in idiopathic autism and that these disruptions were associated with decreased excitatory postsynaptic marker PSD-95, suggesting fewer excitatory synapses. Moreover, we showed that similar molecular deficits were present in VPA-exposed mice that lacked sociability and displayed increased repetitive, stereotyped behaviour. We also determined that behavioural deficits in these mice were rescued by administration of the partial TrkB agonist LM22A-4 but not by treatment with the active tripeptide fragment of the insulin-like growth factor-1, (1-3)IGF-1. Lastly, reduced TrkB signaling in VPA-exposed mice was normalized by LM22A-4 administration combined with behavioural enrichment.
The present work provides a better understanding of the molecular mechanisms that contribute to autistic behaviour and implicates TrkB signaling in autism pathogenesis. Furthermore, these data demonstrate that molecular changes observed in brains of patients with idiopathic autism differ from syndromic forms and highlight that both too much and too little signaling can be equally disruptive. The present work also shows that maternal challenge with VPA resulted in social deficits, increased repetitive, restrictive behaviour and reduced TrkB signaling in mice, pointing to epigenetic modifications as a potential underlying mechanism of molecular and behavioural disruptions in autism. Lastly, these findings suggest that pharmacological activation of TrkB using compounds such as the partial TrkB agonist LM22A-4 might play a role in treating sociability and repetitive, perseverative behaviour in autism.
Thesis
Doctor of Philosophy (PhD)
Advisors/Committee Members: Fahnestock, Margaret, Medical Sciences (Division of Physiology/Pharmacology).
Subjects/Keywords: Autism; LM22A-4; TrkB; VPA; Animal Model
Record Details
Similar Records
Cite
Share »
Record Details
Similar Records
Cite
« Share





❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Nicolini, C. (2016). DEFECTIVE TrkB SIGNALING PATHWAYS IN IDIOPATHIC AUTISM. (Doctoral Dissertation). McMaster University. Retrieved from http://hdl.handle.net/11375/20034
Chicago Manual of Style (16th Edition):
Nicolini, Chiara. “DEFECTIVE TrkB SIGNALING PATHWAYS IN IDIOPATHIC AUTISM.” 2016. Doctoral Dissertation, McMaster University. Accessed February 28, 2021.
http://hdl.handle.net/11375/20034.
MLA Handbook (7th Edition):
Nicolini, Chiara. “DEFECTIVE TrkB SIGNALING PATHWAYS IN IDIOPATHIC AUTISM.” 2016. Web. 28 Feb 2021.
Vancouver:
Nicolini C. DEFECTIVE TrkB SIGNALING PATHWAYS IN IDIOPATHIC AUTISM. [Internet] [Doctoral dissertation]. McMaster University; 2016. [cited 2021 Feb 28].
Available from: http://hdl.handle.net/11375/20034.
Council of Science Editors:
Nicolini C. DEFECTIVE TrkB SIGNALING PATHWAYS IN IDIOPATHIC AUTISM. [Doctoral Dissertation]. McMaster University; 2016. Available from: http://hdl.handle.net/11375/20034

North Carolina State University
12.
Liu, Zifei.
Measurement and Modeling Ammonia Emissions from Broiler Litter.
Degree: PhD, Biological and Agricultural Engineering, 2009, North Carolina State University
URL: http://www.lib.ncsu.edu/resolver/1840.16/5458
► Ammonia is a very important atmospheric pollutant. Agricultural activities, livestock production in particular, have been reported to be the largest contributor of ammonia emissions into…
(more)
▼ Ammonia is a very important atmospheric pollutant. Agricultural activities, livestock production in particular, have been reported to be the largest contributor of ammonia emissions into the atmosphere. Accurate estimation of ammonia emission rate from individual operations or sources is important and yet a challenging task for both regulatory agencies and
animal producers. The overall research objective of this study was to develop an emission
model which can be used to estimate ammonia emission from broiler litter. In the reported
model, the ammonia flux is essentially a function of the litter's total ammoniacal nitrogen (TAN) content, moisture content, pH, and temperature, as well as the Freundlich partition coefficient (Kf), mass transfer coefficient (KG), ventilation rate (Q), and emission surface area (A). A dynamic flow-through chamber system and a wind tunnel were designed to measure ammonia fluxes from broiler litter. The dynamic flow-through chamber experiments evaluated the reported
model with various litter samples under a constant temperature and wind profile. The wind tunnel experiments evaluated the reported
model under various temperatures and wind profiles.
Model parameters such as Kf and KG were estimated. The results from the two experiments were consistent with each other. The estimated KG ranged from 1.11 to 27.64 m h-1, and the estimated Kf ranged from 0.56 to 4.48 L kg-1. Regression sub-models were developed to estimate Kf as a function of litter pH and temperature and to estimate KG as a function of air velocity and temperature. Sensitivity analysis of the
model showed that ammonia flux is very sensitive to litter pH and to a lesser extent temperature. A validation metric based on the mean and covariance in the measurement and in the
model parameters were used to validate the ammonia emission
model in the presence of measurement and
model parameter uncertainties.
Advisors/Committee Members: Peter Bloomfield, Committee Member (advisor), Sanjay Shah, Committee Member (advisor), David Beasley , Committee Co-Chair (advisor), Lingjuan Wang, Committee Chair (advisor).
Subjects/Keywords: emission; model; ammonia; animal feeding operation
Record Details
Similar Records
Cite
Share »
Record Details
Similar Records
Cite
« Share





❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Liu, Z. (2009). Measurement and Modeling Ammonia Emissions from Broiler Litter. (Doctoral Dissertation). North Carolina State University. Retrieved from http://www.lib.ncsu.edu/resolver/1840.16/5458
Chicago Manual of Style (16th Edition):
Liu, Zifei. “Measurement and Modeling Ammonia Emissions from Broiler Litter.” 2009. Doctoral Dissertation, North Carolina State University. Accessed February 28, 2021.
http://www.lib.ncsu.edu/resolver/1840.16/5458.
MLA Handbook (7th Edition):
Liu, Zifei. “Measurement and Modeling Ammonia Emissions from Broiler Litter.” 2009. Web. 28 Feb 2021.
Vancouver:
Liu Z. Measurement and Modeling Ammonia Emissions from Broiler Litter. [Internet] [Doctoral dissertation]. North Carolina State University; 2009. [cited 2021 Feb 28].
Available from: http://www.lib.ncsu.edu/resolver/1840.16/5458.
Council of Science Editors:
Liu Z. Measurement and Modeling Ammonia Emissions from Broiler Litter. [Doctoral Dissertation]. North Carolina State University; 2009. Available from: http://www.lib.ncsu.edu/resolver/1840.16/5458

University of Toronto
13.
Zdravic, Darko.
Investigation of the Pathophysiological Mechanisms of Intracranial Hemorrhage and Miscarriage in alphaIIb Integrin Mediated Fetal and Neonatal Alloimmune Thrombocytopenia.
Degree: 2017, University of Toronto
URL: http://hdl.handle.net/1807/76676
► Investigation of the Pathophysiological Mechanisms of Intracranial Hemorrhage and Miscarriage in anti-alphaIIb Integrin Mediated Fetal and Neonatal Alloimmune Thrombocytopenia Darko Zdravic Master of Science Department…
(more)
▼ Investigation of the Pathophysiological Mechanisms of Intracranial Hemorrhage and Miscarriage in anti-alphaIIb Integrin Mediated Fetal and Neonatal Alloimmune Thrombocytopenia
Darko Zdravic
Master of Science
Department of Laboratory Medicine and Pathobiology
University of Toronto
2017
Fetal and neonatal alloimmune thrombocytopenia (FNAIT) is a life threatening bleeding disorder often leading to bleeding diatheses including intracranial hemorrhage (ICH). Miscarriages have also been reported; however the frequency of pregnancy loss has not been investigated.
Approximately 85% of FNAIT cases are anti-beta3 mediated, whereas anti-alphaIIb mediated FNAIT makes up 3-5% of cases, although their polymorphic frequencies are similar.
We hypothesized that anti-alphaIIb antibodies target trophoblasts during pregnancy, causing impaired placental perfusion and miscarriage in alphaIIb mediated FNAIT. Miscarriages were observed in the majority of immunized alphaIIb-/- mothers, and their placentae showed ischemic appearances with vascular and structural abnormalities, poor perfusion in the labyrinths, and decreased circulating fetal erythrocytes. Neonates developed severe thrombocytopenia and ICH. In vitro experiments demonstrated that anti-alphaIIb antisera significantly inhibited endothelial cell migration and invasion. The results of our studies suggest that a lower reported incidence of anti- alphaIIb mediated FNAIT may be due to miscarriage.
M.Sc.
Advisors/Committee Members: Ni, Heyu, Laboratory Medicine and Pathobiology.
Subjects/Keywords: animal model; antibody; placenta; platelets; thrombocytopenia;
Record Details
Similar Records
Cite
Share »
Record Details
Similar Records
Cite
« Share





❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Zdravic, D. (2017). Investigation of the Pathophysiological Mechanisms of Intracranial Hemorrhage and Miscarriage in alphaIIb Integrin Mediated Fetal and Neonatal Alloimmune Thrombocytopenia. (Masters Thesis). University of Toronto. Retrieved from http://hdl.handle.net/1807/76676
Chicago Manual of Style (16th Edition):
Zdravic, Darko. “Investigation of the Pathophysiological Mechanisms of Intracranial Hemorrhage and Miscarriage in alphaIIb Integrin Mediated Fetal and Neonatal Alloimmune Thrombocytopenia.” 2017. Masters Thesis, University of Toronto. Accessed February 28, 2021.
http://hdl.handle.net/1807/76676.
MLA Handbook (7th Edition):
Zdravic, Darko. “Investigation of the Pathophysiological Mechanisms of Intracranial Hemorrhage and Miscarriage in alphaIIb Integrin Mediated Fetal and Neonatal Alloimmune Thrombocytopenia.” 2017. Web. 28 Feb 2021.
Vancouver:
Zdravic D. Investigation of the Pathophysiological Mechanisms of Intracranial Hemorrhage and Miscarriage in alphaIIb Integrin Mediated Fetal and Neonatal Alloimmune Thrombocytopenia. [Internet] [Masters thesis]. University of Toronto; 2017. [cited 2021 Feb 28].
Available from: http://hdl.handle.net/1807/76676.
Council of Science Editors:
Zdravic D. Investigation of the Pathophysiological Mechanisms of Intracranial Hemorrhage and Miscarriage in alphaIIb Integrin Mediated Fetal and Neonatal Alloimmune Thrombocytopenia. [Masters Thesis]. University of Toronto; 2017. Available from: http://hdl.handle.net/1807/76676
14.
Caulder, Erin.
Metabotropic glutamate receptors as targets for antiepileptic drug therapy: a behavioral and electroencephalographic analysis.
Degree: 2013, Wake Forest University
URL: http://hdl.handle.net/10339/38534
► Epilepsy is a relatively common neurological disorder that involves recurrent seizures thought to be caused by an imbalance in the excitatory and inhibitory systems in…
(more)
▼ Epilepsy is a relatively common neurological disorder that involves recurrent seizures thought to be caused by an imbalance in the excitatory and inhibitory systems in the brain, in favor of excitation. Unfortunately, some types of epilepsy have drug resistance rates of up to 40%. Classically, targets for antiepileptic drugs have included glutamatergic ion channels. However, interfering with excitatory signalling at these channels can alter normal neural communication and can have negative side effects, including cognitive dysfunction and fatigue, which are among the most common complaints from patients. Given the high rate of drug-resistant patients, and the high rate of deleterious side effects, there is an obvious need to develop novel drugs with novel targets and mechanisms of action. Compounds targeting metabotropic glutamate receptors (mGluRs; specifically mGlur2 and 3) have shown promise in other hyperexcitatory neural disorders, and some have shown efficacy in models of epilepsy as well. The goal of these studies was to demonstrate a conclusive behavioral effect of particular mGluR active compounds in reducing the severity of seizures associated with two models of epilepsy. Both models that were used are models of generalized seizure, although one is characterized by convulsive seizures (pilocarpine model) and the other is characterized by the lack of convulsive seizure, or an "absence" seizure (GBL model). Mice were given mGluR2/3 active drugs either before or after seizure onset and behavioral observations relating to seizure were measured. In some cases, the mice had been implanted with tethered EEG devices to record neural activity that coincided with behavioral observations and measures. It was demonstrated that in generalized models of seizure, mGluR2/3 active agonists had some efficacy at reducing seizure severity. The effect of modulators at mGluR2 was also investigated. One in particular, CBiPES had a modest but significant effect in reducing the behavioral severity of pilocarpine induced seizures, but not of GBL induced seizures. In conclusion, mGluR2/3 remains a valid target for antiepileptic drug development. The ideal compound would yield higher response rates for patients while boasting a minimal negative side effect profile.
Subjects/Keywords: animal model
…considered a valid model of absense:
the behavior of the epileptic animal is similar to epileptic… …seizure) has been used in animals as a model of epilepsy for decades (Racine,
1978… …involved in epileptogenesis and in seizures are
relatively well known and understood. Animal… …rodent model
systems offers many theories. Epileptic seizures can result in the excitotoxic… …support the epileptogenic process in the
human and experimental animal brain.
2. Channelopathies…
Record Details
Similar Records
Cite
Share »
Record Details
Similar Records
Cite
« Share





❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Caulder, E. (2013). Metabotropic glutamate receptors as targets for antiepileptic drug therapy: a behavioral and electroencephalographic analysis. (Thesis). Wake Forest University. Retrieved from http://hdl.handle.net/10339/38534
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Caulder, Erin. “Metabotropic glutamate receptors as targets for antiepileptic drug therapy: a behavioral and electroencephalographic analysis.” 2013. Thesis, Wake Forest University. Accessed February 28, 2021.
http://hdl.handle.net/10339/38534.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Caulder, Erin. “Metabotropic glutamate receptors as targets for antiepileptic drug therapy: a behavioral and electroencephalographic analysis.” 2013. Web. 28 Feb 2021.
Vancouver:
Caulder E. Metabotropic glutamate receptors as targets for antiepileptic drug therapy: a behavioral and electroencephalographic analysis. [Internet] [Thesis]. Wake Forest University; 2013. [cited 2021 Feb 28].
Available from: http://hdl.handle.net/10339/38534.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Caulder E. Metabotropic glutamate receptors as targets for antiepileptic drug therapy: a behavioral and electroencephalographic analysis. [Thesis]. Wake Forest University; 2013. Available from: http://hdl.handle.net/10339/38534
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Otago
15.
Hegan, William Matthew.
Gambling Behaviour in Pigeons: Toward an Animal Model of Gambling
.
Degree: 2011, University of Otago
URL: http://hdl.handle.net/10523/1646
► The gambling addiction is a destructive impulse control disorder that leaves families destitute and lives in ruins. The treatment and pathology of gambling has become…
(more)
▼ The gambling addiction is a destructive impulse control disorder that leaves families destitute and lives in ruins. The treatment and pathology of gambling has become a popular area of research, reflected in national media campaigns here in New Zealand. Studies suggest that there are prefrontal activation differences in problem gamblers compared to healthy volunteers, areas involved with decision making and reward.
Animal research has begun to investigate an
animal model of gambling, with some studies showing behavioural similarities between animals and humans when playing gambling tasks.
The current thesis sought to further investigate an
animal model of gambling, performing two experiments using a slot machine task. Experiment 1 was an investigation into the neural basis of gambling, examining the behaviour of single neurons in the avian NCL, an equivalent to the mammalian prefrontal cortex. Four pigeons (Columba livia) served as subjects and played a touch screen slot machine task, similar to a slot machine found in any casino. Pigeons were required to peck an upright arm to initiate each trial, and then peck four rolling tumblers in succession from left to right. If four identical stimuli appeared, then a wheat reward was won. During the task, activity from single NCL neurons was recorded. Four neuronal types were found to correlate with gambling related behaviour: Reward Proximity neurons, I-Won neurons, I-Lost neurons and Near Win neurons. In addition pigeons were split into two groups with one group trained for a short (one month) and long (four month) period, in an attempt to mimic naive and problem gamblers. It was hypothesised that due to prolonged training the two groups would differ in the amount and magnitude of firing of these neurons. Results showed no statistical difference between groups in either amount of gambling neurons or magnitude of firing. Behavioural data were also collected and it was hypothesised that all birds would show evidence of a post reinforcement pause, something our results confirmed.
Experiment 2 investigated whether pigeons, similar to humans, show a preference to play slot machines with higher near win ratios. Four pigeons were again tested on a touch screen slot machine task, required to play on two different ‘machines’ differentiated by different coloured backgrounds. It was hypothesised that when pigeons were given a choice, they would choose the machine that had the higher percentage of near win trials. Our results did not show evidence of any preference, and it was concluded that the near win effect may not be replicable in animals.
In conclusion, the current thesis demonstrates that neurons in the avian NCL code for gambling behaviours while pigeons play a slot machine task. Although an analogue of slot machine gambling may be found in animals, our findings suggest gambling characteristics such as the near win effect may not be replicable. The current findings provide a base for further research to investigate an
animal model of gambling.
Advisors/Committee Members: Colombo, Michael (advisor).
Subjects/Keywords: Gambling Research;
Gambling;
Avian;
Pigeon;
Animal Model
Record Details
Similar Records
Cite
Share »
Record Details
Similar Records
Cite
« Share





❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Hegan, W. M. (2011). Gambling Behaviour in Pigeons: Toward an Animal Model of Gambling
. (Masters Thesis). University of Otago. Retrieved from http://hdl.handle.net/10523/1646
Chicago Manual of Style (16th Edition):
Hegan, William Matthew. “Gambling Behaviour in Pigeons: Toward an Animal Model of Gambling
.” 2011. Masters Thesis, University of Otago. Accessed February 28, 2021.
http://hdl.handle.net/10523/1646.
MLA Handbook (7th Edition):
Hegan, William Matthew. “Gambling Behaviour in Pigeons: Toward an Animal Model of Gambling
.” 2011. Web. 28 Feb 2021.
Vancouver:
Hegan WM. Gambling Behaviour in Pigeons: Toward an Animal Model of Gambling
. [Internet] [Masters thesis]. University of Otago; 2011. [cited 2021 Feb 28].
Available from: http://hdl.handle.net/10523/1646.
Council of Science Editors:
Hegan WM. Gambling Behaviour in Pigeons: Toward an Animal Model of Gambling
. [Masters Thesis]. University of Otago; 2011. Available from: http://hdl.handle.net/10523/1646

Colorado State University
16.
Soffler, Carl.
Development and characterization of caprine infection models of melioidosis, The.
Degree: PhD, Microbiology, Immunology, and Pathology, 2012, Colorado State University
URL: http://hdl.handle.net/10217/71589
► Melioidosis, the disease resulting from infection with Burkholderia pseudomallei, is a serious emerging infectious disease endemic to Southeast Asia and Northern Australasia and a leading…
(more)
▼ Melioidosis, the disease resulting from infection with Burkholderia pseudomallei, is a serious emerging infectious disease endemic to Southeast Asia and Northern Australasia and a leading infectious cause of death in the former. Additionally, B. pseudomallei has been designated a Category B Select Agent by the United States Centers for Disease Control and Prevention because of its potential use in bioterrorism, which has led to intensive research on inhalational models of murine melioidosis. Natural infection is believed to occur predominantly through percutaneous inoculation or inhalation in the rainy season in endemic areas, with infection following oral exposure occurring to a lesser extent. However, the actual importance of each route of infection in natural disease is unknown. Studies examining the comparative pathogenesis of melioidosis in regards to the route of infection are generally lacking, particularly in naturally affected species. A goat
model was selected as it provides the opportunity to study the importance of the route of infection and its effect on disease pathogenesis in a naturally affected species. Disease and outcome can be evaluated relative to natural presentations in both human and goat populations as goats and humans exhibit a similar epizootiology/epidemiology of melioidosis, which corresponds to similar environmental exposure to B. pseudomallei within its endemic range. Furthermore, the larger body size of goats allows for human-relevant clinical monitoring as well as longer-term serial evaluation of disease progression and therapy in individual animals. Using a caprine
model system, we have investigated the pathogenesis of infection following intratracheal aerosol and percutaneous exposure to 104 delivered colony forming units (CFU) of B. pseudomallei. Disease was observed in all animals following infection. Acute disease was more severe in aerosol infected goats, but both groups tended to develop subacute to chronic active disease, with percutaneously infected goats showing regression of lesions at the later time points. Percutaneously infected goats generally exhibited more variable clinical signs, hematologic changes, and gross pathology, but often had histologic lesions with more severe changes. Dissemination from the site of infection was much more rapid in the percutaneously infected animals, with bacteria detectable in the lungs and spleen as early as Day 2 post-infection (PI) and gross abscessation evident in distant sites as early as Day 7 PI. Extrapulmonary dissemination after aerosol infection appeared to occur around Day 7 with splenic or renal abscesses not grossly detectable until day 14. Lesion development was closely associated with a leukocytoclastic vasculitis observed in affected tissues in both aerosol and percutaneous infection. Pulmonary involvement was evident in all but one percutaneously infected goat (Day 2 PI) by culture or the presence of histologic lesions. The rapid dissemination of B. pseudomallei after percutaneous inoculation challenges the perception…
Advisors/Committee Members: Bowen, Richard A. (advisor), Aboellail, Tawfik A. (committee member), Dow, Steven S. (committee member), Schweizer, Herbert P. (committee member), Van Metre, David C. (committee member).
Subjects/Keywords: animal model; pseudomallei; percutaneous; melioidosis; pathogenesis; aerosol
Record Details
Similar Records
Cite
Share »
Record Details
Similar Records
Cite
« Share





❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Soffler, C. (2012). Development and characterization of caprine infection models of melioidosis, The. (Doctoral Dissertation). Colorado State University. Retrieved from http://hdl.handle.net/10217/71589
Chicago Manual of Style (16th Edition):
Soffler, Carl. “Development and characterization of caprine infection models of melioidosis, The.” 2012. Doctoral Dissertation, Colorado State University. Accessed February 28, 2021.
http://hdl.handle.net/10217/71589.
MLA Handbook (7th Edition):
Soffler, Carl. “Development and characterization of caprine infection models of melioidosis, The.” 2012. Web. 28 Feb 2021.
Vancouver:
Soffler C. Development and characterization of caprine infection models of melioidosis, The. [Internet] [Doctoral dissertation]. Colorado State University; 2012. [cited 2021 Feb 28].
Available from: http://hdl.handle.net/10217/71589.
Council of Science Editors:
Soffler C. Development and characterization of caprine infection models of melioidosis, The. [Doctoral Dissertation]. Colorado State University; 2012. Available from: http://hdl.handle.net/10217/71589

University of Adelaide
17.
Lett, Bron Douglas.
[EMBARGOED] Mesenchymal stem cells in an ovine model of kidney transplantation.
Degree: 2018, University of Adelaide
URL: http://hdl.handle.net/2440/120861
► Mesenchymal stem cells (MSCs) have the potential to address current issues in transplantation with their immunosuppressive and regenerative abilities. MSCs as a therapy to improve…
(more)
▼ Mesenchymal stem cells (MSCs) have the potential to address current issues in transplantation with their immunosuppressive and regenerative abilities. MSCs as a therapy to improve kidney transplant outcomes have already been taken to the clinical trial stage. The results of these trials have, unfortunately, been disappointing with no significant improvements seen over current transplant outcomes. A return to basic science will help to answer many of the questions surrounding the best methods for the use of MSCs in transplantation medicine. To this end this thesis has set out to examine the application of MSCs in a sheep
model of heterotopic kidney transplantation. Chapter 3 describes the isolation, characterisation, and labelling of MSCs with a superparamagnetic iron oxide (SPIO) nano particle that can be used to track the cells using MRI and Prussian blue staining. Importantly, labelling the cells with the SPIO did not have an impact on their phenotype or function. In Chapter 5, the SPIO-MSCs then had their migration tracked when delivered systemically in a sheep kidney autotransplantation
model, the development of which is described in chapter 4. The observations indicated the cells passed through the transplanted kidney at 15 minutes post administration but had dispersed by 30 minutes. Upon histological study, significantly more cells had been found to localize to the transplanted kidney than to the native kidney. Lastly, chapter 6 details the effects of MSCs delivered into the artery of kidney allografts. Looking at the creatinine, urea, and kim-1 levels of sheep early after transplantation and later undergoing rejection, gave suggestions that MSCs could reduce kim-1 levels soon after transplantation and may reduce some aspects of rejection. Additionally, the MSCs did not negatively impact the transplanted kidney, demonstrating the safety of delivering the cells arterially.
Advisors/Committee Members: Coates, Toby (advisor), Drogemuller, Chris (advisor), Adelaide Medical School (school).
Subjects/Keywords: Stem cells; kidney transplantation; animal model
Record Details
Similar Records
Cite
Share »
Record Details
Similar Records
Cite
« Share





❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Lett, B. D. (2018). [EMBARGOED] Mesenchymal stem cells in an ovine model of kidney transplantation. (Thesis). University of Adelaide. Retrieved from http://hdl.handle.net/2440/120861
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Lett, Bron Douglas. “[EMBARGOED] Mesenchymal stem cells in an ovine model of kidney transplantation.” 2018. Thesis, University of Adelaide. Accessed February 28, 2021.
http://hdl.handle.net/2440/120861.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Lett, Bron Douglas. “[EMBARGOED] Mesenchymal stem cells in an ovine model of kidney transplantation.” 2018. Web. 28 Feb 2021.
Vancouver:
Lett BD. [EMBARGOED] Mesenchymal stem cells in an ovine model of kidney transplantation. [Internet] [Thesis]. University of Adelaide; 2018. [cited 2021 Feb 28].
Available from: http://hdl.handle.net/2440/120861.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Lett BD. [EMBARGOED] Mesenchymal stem cells in an ovine model of kidney transplantation. [Thesis]. University of Adelaide; 2018. Available from: http://hdl.handle.net/2440/120861
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

AUT University
18.
Al-Rawi, Mohammad.
Computational fluid dynamic modelling for arterial diseases assessment
.
Degree: 2013, AUT University
URL: http://hdl.handle.net/10292/5202
► One of the leading causes of death is cardiovascular disease, being 30% of all deaths worldwide and 40% of those in New Zealand. In other…
(more)
▼ One of the leading causes of death is cardiovascular disease, being 30% of all deaths worldwide and 40% of those in New Zealand. In other words, every 90 minutes a New Zealander dies due to cardiovascular disease. Cardiovascular mortality is higher in New Zealand than Australia, but the reasons for this are not clear. Although Australia and New Zealand are similar politically, culturally, and socioeconomically, mortality from cardiovascular disease is about 25% higher in New Zealand than in Australia.
In recent years, engineers and scientists have collaborated with the medical community to find new methods and approaches for assessing and investigating the development of cardiovascular diseases such as abdominal aortic aneurysm and atherosclerosis. In this thesis, atherosclerosis and aneurysm diseases are investigated and analysed using computational fluid dynamic/finite element (CFD/FE) methods. These models are validated against the in vivo and in vitro experiments performed on animals. The experimental models are also investigated; the assessment of arterial blockages using blood pressure waveforms obtained invasively at the right femoral artery.
The
animal experiments are performed following appropriate ethical protocols (R915) on Wistar rats weighing between 250-350g. An arterial blockage is created surgically within the abdominal aorta of healthy animals to create an unhealthy condition. Blood pressure waveforms are measured by injecting catheter into the right femoral artery of the rat. These measurements are taken at the baseline (healthy condition) and at four different severities of arterial blockage of the abdominal aorta for the same specimen. In vivo and in vitro measurements of the arterial diameter and wall thickness are also taken using Magnetic Resonance Imaging (MRI) and microscopic techniques, respectively.
These data are then input onto CFD/FE models in order to develop a new, non-invasive method of assessing arterial blockage. The experimental and computational results indicate that arterial blockages occurring within the abdominal aorta could be assessed, and the development of the disease diagnosed, very clearly and non-invasively at the right femoral artery. The findings of the
animal model are then implemented in the human
model for screening atherosclerosis and aneurysm at the brachial artery. These diseases are modelled and simulated in a 3D CFD/FE aorta geometry using the fluid–structure interaction (FSI) approach on the commercial software ANSYS®14.0. Literature blood flow waveform datum is assumed at the inlet and invasive catheter pulsatile pressure waveforms data is imposed at the four outlets of the aorta (provided by Green Lane Hospital under ethic approval number NTX/09/11/109). Correlations between the stress phase angle (SPA), augmentation index (AI), lumen diameter and blood pressure waveforms for various scenarios of diseased models are made and compared to the control
model. The results show that CFD/FE models with different radii and thicknesses at the abdominal…
Advisors/Committee Members: Al-Jumaily, Ahmed (advisor), Lowe, Andrew (advisor).
Subjects/Keywords: CFD/FE;
Animal model;
Arterial diseases
Record Details
Similar Records
Cite
Share »
Record Details
Similar Records
Cite
« Share





❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Al-Rawi, M. (2013). Computational fluid dynamic modelling for arterial diseases assessment
. (Thesis). AUT University. Retrieved from http://hdl.handle.net/10292/5202
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Al-Rawi, Mohammad. “Computational fluid dynamic modelling for arterial diseases assessment
.” 2013. Thesis, AUT University. Accessed February 28, 2021.
http://hdl.handle.net/10292/5202.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Al-Rawi, Mohammad. “Computational fluid dynamic modelling for arterial diseases assessment
.” 2013. Web. 28 Feb 2021.
Vancouver:
Al-Rawi M. Computational fluid dynamic modelling for arterial diseases assessment
. [Internet] [Thesis]. AUT University; 2013. [cited 2021 Feb 28].
Available from: http://hdl.handle.net/10292/5202.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Al-Rawi M. Computational fluid dynamic modelling for arterial diseases assessment
. [Thesis]. AUT University; 2013. Available from: http://hdl.handle.net/10292/5202
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Oxford
19.
Coutinho, Maria Ester Freitas Barbosa Pereira.
Central nervous system autoimmunity in neuropsychiatric disorders.
Degree: PhD, 2016, University of Oxford
URL: http://ora.ox.ac.uk/objects/uuid:389fb830-4b4e-4201-9965-19acb2c63ff3
;
https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.729904
► The recent history of autoimmune neurology is marked by the discovery of many central nervous system (CNS) antibody-mediated diseases. These disorders are caused by antibodies…
(more)
▼ The recent history of autoimmune neurology is marked by the discovery of many central nervous system (CNS) antibody-mediated diseases. These disorders are caused by antibodies that target important proteins expressed in the neuronal surface, which are believed to be directly pathogenic. These antibodies are immunoglobulin G (IgG) isotype and, as such, have the potential to cross the placenta during gestation. Foetal exposure to CNS-targeting antibodies could alter developing neuronal circuits, leading to disease. However, the consequences of exposure to these antibodies during neurodevelopment has hardly been considered. To study the relationship between maternal antibodies towards neuronal surface proteins and neurodevelopmental disorders in the foetus a dual approach was undertaken. First, pregnancy serum samples from mothers of children later diagnosed with a neurodevelopmental disorder and from mothers of children with typical development were screened for the presence of neuronal surface antibodies. Next, the effects of pathogenic neuronal surface antibodies in the offspring were assessed in a maternal-to-foetal transfer mouse model. Antibodies to neuronal surface proteins in the gestational serum, particularly CASPR2 antibodies, were found to associate with an increased risk of mental retardation and disorders of psychological development in the progeny. The animal model showed that mice exposed in utero to CASPR2 antibodies have long term behavioural sequelae and histological findings suggestive of abnormalities in brain development. These findings support a model in which maternal antibodies towards foetal neuronal proteins cause long-term behavioural deficits and permanent abnormalities at the cellular and synaptic level in a subset of children with neurodevelopmental disorders.
Subjects/Keywords: 616.8; Neuroimmunology; Neurodevelopmental disorders; Autoantibodies; animal model
Record Details
Similar Records
Cite
Share »
Record Details
Similar Records
Cite
« Share





❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Coutinho, M. E. F. B. P. (2016). Central nervous system autoimmunity in neuropsychiatric disorders. (Doctoral Dissertation). University of Oxford. Retrieved from http://ora.ox.ac.uk/objects/uuid:389fb830-4b4e-4201-9965-19acb2c63ff3 ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.729904
Chicago Manual of Style (16th Edition):
Coutinho, Maria Ester Freitas Barbosa Pereira. “Central nervous system autoimmunity in neuropsychiatric disorders.” 2016. Doctoral Dissertation, University of Oxford. Accessed February 28, 2021.
http://ora.ox.ac.uk/objects/uuid:389fb830-4b4e-4201-9965-19acb2c63ff3 ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.729904.
MLA Handbook (7th Edition):
Coutinho, Maria Ester Freitas Barbosa Pereira. “Central nervous system autoimmunity in neuropsychiatric disorders.” 2016. Web. 28 Feb 2021.
Vancouver:
Coutinho MEFBP. Central nervous system autoimmunity in neuropsychiatric disorders. [Internet] [Doctoral dissertation]. University of Oxford; 2016. [cited 2021 Feb 28].
Available from: http://ora.ox.ac.uk/objects/uuid:389fb830-4b4e-4201-9965-19acb2c63ff3 ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.729904.
Council of Science Editors:
Coutinho MEFBP. Central nervous system autoimmunity in neuropsychiatric disorders. [Doctoral Dissertation]. University of Oxford; 2016. Available from: http://ora.ox.ac.uk/objects/uuid:389fb830-4b4e-4201-9965-19acb2c63ff3 ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.729904

University of Manchester
20.
Oladipo, Joanna.
The development and validation of a maternal immune activation (mIA) animal model relevant to neurodevelopmental disorders : a focus towards Autism Spectrum Disorder (ASD).
Degree: PhD, 2018, University of Manchester
URL: https://www.research.manchester.ac.uk/portal/en/theses/the-development-and-validation-of-a-maternal-immune-activation-mia-animal-model-relevant-to-neurodevelopmental-disorders-a-focus-towards-autism-spectrum-disorder-asd(aa39ca74-6972-430f-83d1-e77e0a1418c5).html
;
https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.771375
► Autism spectrum disorder (ASD) is a pervasive neurodevelopmental disorder (NDD) which, despite much research, is not well understood. The heterogeneity of ASD patient cohorts supports…
(more)
▼ Autism spectrum disorder (ASD) is a pervasive neurodevelopmental disorder (NDD) which, despite much research, is not well understood. The heterogeneity of ASD patient cohorts supports the hypothesis of complex interactions of genetic and environmental mechanisms in its aetiology and severity. The presence of gastrointestinal (GI) disturbances in ASD patients that disrupt the bi-directional signalling of the gut-brain axis, suggest that this may play a role in the manifestation of ASD phenotypes. There is also accumulating evidence for the role of neuroinflammatory processes, in both pregnant mother and affected offspring, in the aetiology of NDDs. The increased risk of ASD following infection during pregnancy highlights maternal immune activation (mIA) as a key animal model for NDDs (notably for schizophrenia and ASD) where relevant behavioural phenotypes manifest in the offspring of mIA dams. ASD remains a poorly managed NDD, with no evidence-based therapies for prevention or treatment currently available. Validating new mIA models and refining current methods is vital to the development of new therapeutic strategies. This thesis has explored the development of an mIA model to test the maternal infection hypothesis using exposure to the viral mimetic polyinosinic-polycytidylic acid (poly (I:C)) administered mid-way through pregnancy (gestational day (GD) 12.5) in rats. First, the acute effects of poly (I:C) in non-pregnant female rats were investigated, providing a validation of the experimental methods (strain, route of administration and dose). Separate cohorts of Wistar rats were then used in the GD12.5 mIA model and the physiological responses to 10 mg/kg i.p. poly (I:C) measured. Various measures including elevation of plasma IL-6 and changes in core body temperature were found to be variable between treated dams. These results confirmed the need to standardise analysis of mIA in dams, to correlate maternal inflammation with subsequent offspring outcomes such as phenotype and neuronal development changes. Poly (I:C) batch differences were also thought to contribute to these variable results and refinement of mIA methods are proposed. Longitudinal effects of mIA on offspring of both sexes were investigated including their developmental trajectory measuring specific gene expression, morphology at GD21 and post-natal day (PD) 21, and behavioural phenotyping at adolescence and adulthood. Gestational and early postnatal changes in offspring following mIA have yet to be systematically explored. Gene expression analysis at GD21 and PD21 in offspring shows that this mIA model is useful for the identification of early developmental changes relevant to NDDs. Moreover, analysis of gene expression changes related to synaptic function, glial cells and blood-brain barrier integrity demonstrated that changes were dependent on both sex and brain region of interest. This confirms the need to include both sexes of offspring and inclusion of different brain regions for analysis which is a common limitation of previous preclinical…
Subjects/Keywords: 610; animal model; neurodevelopmental; maternal immune activation
Record Details
Similar Records
Cite
Share »
Record Details
Similar Records
Cite
« Share





❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Oladipo, J. (2018). The development and validation of a maternal immune activation (mIA) animal model relevant to neurodevelopmental disorders : a focus towards Autism Spectrum Disorder (ASD). (Doctoral Dissertation). University of Manchester. Retrieved from https://www.research.manchester.ac.uk/portal/en/theses/the-development-and-validation-of-a-maternal-immune-activation-mia-animal-model-relevant-to-neurodevelopmental-disorders-a-focus-towards-autism-spectrum-disorder-asd(aa39ca74-6972-430f-83d1-e77e0a1418c5).html ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.771375
Chicago Manual of Style (16th Edition):
Oladipo, Joanna. “The development and validation of a maternal immune activation (mIA) animal model relevant to neurodevelopmental disorders : a focus towards Autism Spectrum Disorder (ASD).” 2018. Doctoral Dissertation, University of Manchester. Accessed February 28, 2021.
https://www.research.manchester.ac.uk/portal/en/theses/the-development-and-validation-of-a-maternal-immune-activation-mia-animal-model-relevant-to-neurodevelopmental-disorders-a-focus-towards-autism-spectrum-disorder-asd(aa39ca74-6972-430f-83d1-e77e0a1418c5).html ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.771375.
MLA Handbook (7th Edition):
Oladipo, Joanna. “The development and validation of a maternal immune activation (mIA) animal model relevant to neurodevelopmental disorders : a focus towards Autism Spectrum Disorder (ASD).” 2018. Web. 28 Feb 2021.
Vancouver:
Oladipo J. The development and validation of a maternal immune activation (mIA) animal model relevant to neurodevelopmental disorders : a focus towards Autism Spectrum Disorder (ASD). [Internet] [Doctoral dissertation]. University of Manchester; 2018. [cited 2021 Feb 28].
Available from: https://www.research.manchester.ac.uk/portal/en/theses/the-development-and-validation-of-a-maternal-immune-activation-mia-animal-model-relevant-to-neurodevelopmental-disorders-a-focus-towards-autism-spectrum-disorder-asd(aa39ca74-6972-430f-83d1-e77e0a1418c5).html ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.771375.
Council of Science Editors:
Oladipo J. The development and validation of a maternal immune activation (mIA) animal model relevant to neurodevelopmental disorders : a focus towards Autism Spectrum Disorder (ASD). [Doctoral Dissertation]. University of Manchester; 2018. Available from: https://www.research.manchester.ac.uk/portal/en/theses/the-development-and-validation-of-a-maternal-immune-activation-mia-animal-model-relevant-to-neurodevelopmental-disorders-a-focus-towards-autism-spectrum-disorder-asd(aa39ca74-6972-430f-83d1-e77e0a1418c5).html ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.771375
21.
Duprez, Jessica Anais Sybille.
Experimental vaccination for onchocerciasis and the identification of early markers of protective immunity.
Degree: PhD, 2018, University of Edinburgh
URL: http://hdl.handle.net/1842/28976
► Onchocerciasis, caused by Onchocerca volvulus remains a major public health and socio-economic problem across the tropics, despite years of mass drug administration (MDA) with Ivermectin…
(more)
▼ Onchocerciasis, caused by Onchocerca volvulus remains a major public health and socio-economic problem across the tropics, despite years of mass drug administration (MDA) with Ivermectin to reduce disease burden. Through modelling, it has been shown that elimination cannot be achieved with MDA alone and additional tools are needed, such as vaccination, which remains the most cost-effective tool for long-term disease control. The feasibility behind vaccination against O. volvulus can be demonstrated in the Litomosoides sigmodontis mouse model, which shows that vaccine induced protection can be achieved with immunisation using irradiated L3, the infective stage of L. sigmodontis and with microfilariae (Mf), the transmission stage of the parasite. There is further evidence of protective immunity in humans, with individuals living in endemic areas that show no signs of infection despite being exposed to the parasite (endemic normal). The protective efficacy of promising vaccine candidates were evaluated using an immunisation time course in the L. sigmodontis model, using either DNA plasmid or peptide vaccines. In immunisation experiments in L. sigmodontis, Mf numbers are used as a measure of protection and marks the end of an immunisation time course. However, when changes in gene expression were measured at the end of an immunisation time course, in attempts to identify gene signatures that could be used as markers of protection (correlates of protection) in the blood, no gene signatures were found to be associated with protection. This suggest that at the end of an immunisation time course, when protection is measured (change in Mf numbers), it is too late in infection to measure changes in immune pathways being triggered. Changes in gene expression were therefore measured in blood samples collected throughout an immunisation time course in the L. sigmodontis model, in order to identify the time point in an immunisation experiment which are the most indicative of protection. Two independent immunisation time courses were used, either using irradiated L3 or Mf as vaccine against L. sigmodontis, as these elicit the greatest protection. This generated a large high dimensional dataset, that was too large and complex for a differential fold-change analysis. Therefore, an analysis pipeline was created using machine learning algorithms, to detect changes in gene expression throughout the time courses to detect markers of protection. The 6 hour time point following immunisation showed the greatest change in gene expression, with the analysis pipeline identifying known pathways associated with vaccine-induced immunity. The pipeline was applied to gene expression data from human samples obtained from individuals living in endemic areas who were either infected with O. volvulus or endemic normal (naturally protected), this was to identify pathways associated with protective immunity in humans. When comparing vaccine induced immunity seen in mice and natural protective immunity in humans there was some overlap in pathways being…
Subjects/Keywords: onchocerciasis; river blindness; vaccination time; animal model
Record Details
Similar Records
Cite
Share »
Record Details
Similar Records
Cite
« Share





❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Duprez, J. A. S. (2018). Experimental vaccination for onchocerciasis and the identification of early markers of protective immunity. (Doctoral Dissertation). University of Edinburgh. Retrieved from http://hdl.handle.net/1842/28976
Chicago Manual of Style (16th Edition):
Duprez, Jessica Anais Sybille. “Experimental vaccination for onchocerciasis and the identification of early markers of protective immunity.” 2018. Doctoral Dissertation, University of Edinburgh. Accessed February 28, 2021.
http://hdl.handle.net/1842/28976.
MLA Handbook (7th Edition):
Duprez, Jessica Anais Sybille. “Experimental vaccination for onchocerciasis and the identification of early markers of protective immunity.” 2018. Web. 28 Feb 2021.
Vancouver:
Duprez JAS. Experimental vaccination for onchocerciasis and the identification of early markers of protective immunity. [Internet] [Doctoral dissertation]. University of Edinburgh; 2018. [cited 2021 Feb 28].
Available from: http://hdl.handle.net/1842/28976.
Council of Science Editors:
Duprez JAS. Experimental vaccination for onchocerciasis and the identification of early markers of protective immunity. [Doctoral Dissertation]. University of Edinburgh; 2018. Available from: http://hdl.handle.net/1842/28976

University of Gothenburg / Göteborgs Universitet
22.
Bian, Li.
The role of S100A4 protein as a regulator of inflammation and bone metabolism in experimental arthritis.
Degree: 2011, University of Gothenburg / Göteborgs Universitet
URL: http://hdl.handle.net/2077/26277
► S100A4 belongs to the family of calcium-binding S100 proteins and modulates cell proliferation, cytoskeletal rearrangement, cell motility, and angiogenesis. Increased levels of S100A4 expression correlate…
(more)
▼ S100A4 belongs to the family of calcium-binding S100 proteins and modulates cell proliferation,
cytoskeletal rearrangement, cell motility, and angiogenesis. Increased levels of S100A4 expression
correlate with high incidence of metastasis of cancers. Up-regulation of S100A4 protein is
demonstrated in synovial tissue and in plasma of rheumatoid arthritis (RA) patients compared with
osteoarthritis, and the elevated expression of S100A4 is associated with increased disease activity in
patients with RA. Dichloroacetate (DCA) was shown to have a potent anti-tumour effect by
facilitating apoptosis and inhibiting proliferation. The aims of this thesis are to investigate
contributions of S100A4 in experimental models of septic arthritis and antigen-induced arthritis, and
in bone formation using S100A4KO mice. In addition, we also aim to find out the impact of DCA on
collagen type II-induced arthritis.
Our studies showed that S100A4 deficiency resulted in reduced joint inflammation and
cartilage/bone destruction in both septic and antigen-induced arthritis in mice. Additionally, in
septic arthritis, S100A4KO mice had less bone loss and showed a lower bacterial load in the kidneys.
S100A4 deficiency resulted in changed pattern of adhesion molecules. In antigen-induced arthritis,
S100A4 deficiency resulted in reduced intensity of arthritis and significantly lower frequency of bone
destruction, supported by fewer numbers of CD4+ T cells and CD19+CD5+ B cells accumulated in
synovia and spleen compared with WT mice. Smaller populations of CD4+ and CD8+ T cells in
spleen of S100A4 deficient mice were accompanied by reduced productions of INF-γ and IL-17A,
and lower expression of Th17 transcription factor RORγt. Difference in the severity of arthritis was
observed in female mice in septic arthritis and in male mice in antigen-induced arthritis. To assess the
role of sex hormone on bone, we analysed BMD in S100A4KO and WT mice. S100A4KO mice had
higher total BMD and female mice displayed more cortical bone content compared with WT mice.
Following ovariectomy (OVX), both S100A4KO and WT mice lost BMD. However, cortical bone loss
was more pronounced in S100A4KO mice than in WT supported by high CTX-I level. The loss of
trabecular bone was similar in S100A4KO and WT mice. DHEA treatment resulted in a significant
increase in the trabecular and cortical BMD both in WT and S100A4KO mice. This increase of BMD
was lower in S100A4KO mice. The collagen-type II arthritis model was employed to study the
potential effect of dichloroacetate (DCA) treatment on experimental arthritis. Our results showed
that mice treated with DCA had a slower onset of CIA, and significantly lower severity and frequency
of joint inflammation and cartilage/bone destruction compared with water-treated controls.
Moreover, DCA prevented arthritis-induced loss of cortical mineral density. The beneficial effect of
DCA was present only in female DBA/1 mice. DCA treatment on the OVX mice did not protect from
the development of arthritis, indicating that effect of DCA is…
Subjects/Keywords: S100A4; arthritis; animal model; bone; inflammation
Record Details
Similar Records
Cite
Share »
Record Details
Similar Records
Cite
« Share





❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Bian, L. (2011). The role of S100A4 protein as a regulator of inflammation and bone metabolism in experimental arthritis. (Thesis). University of Gothenburg / Göteborgs Universitet. Retrieved from http://hdl.handle.net/2077/26277
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Bian, Li. “The role of S100A4 protein as a regulator of inflammation and bone metabolism in experimental arthritis.” 2011. Thesis, University of Gothenburg / Göteborgs Universitet. Accessed February 28, 2021.
http://hdl.handle.net/2077/26277.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Bian, Li. “The role of S100A4 protein as a regulator of inflammation and bone metabolism in experimental arthritis.” 2011. Web. 28 Feb 2021.
Vancouver:
Bian L. The role of S100A4 protein as a regulator of inflammation and bone metabolism in experimental arthritis. [Internet] [Thesis]. University of Gothenburg / Göteborgs Universitet; 2011. [cited 2021 Feb 28].
Available from: http://hdl.handle.net/2077/26277.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Bian L. The role of S100A4 protein as a regulator of inflammation and bone metabolism in experimental arthritis. [Thesis]. University of Gothenburg / Göteborgs Universitet; 2011. Available from: http://hdl.handle.net/2077/26277
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
23.
Salmeto-Johnson, Amy L.
Measurement Of Anhedonia In The Chick Anxiety-Depression Model.
Degree: PhD, Psychology, 2014, University of Mississippi
URL: https://egrove.olemiss.edu/etd/838
► Anhedonia, the loss of pleasure in previously pleasurable activities, is one of the cardinal features of depression. To further validate the chick anxiety-depression model, the…
(more)
▼ Anhedonia, the loss of pleasure in previously pleasurable activities, is one of the cardinal features of depression. To further validate the chick anxiety-depression
model, the current study aimed at quantifying anhedonia as well its reversal with pharmaceuticals. The first goal was to identify a measure to quantify the display of anhedonia in chicks following exposure to an isolation stressor, the chick anxiety-depression
model. All experiments involved a baseline and test measurement either after removal from the home cage (No Test) or exposure to the isolation apparatus with conspecifics and mirrors (Social) or individually (Isolated). Experiment 1 used a straight maze with start and goal latency serving as the dependent measures. Isolated chicks expressed delays in start latency compared to No Test chicks, which is interpreted as anhedonia-like behavior. Experiment 2 used a modified sucrose preference task to assess sucrose preference and the number of drinking events for water and sucrose. Consistent with the rodent literature, results showed decreased sucrose preference in Isolated chicks compared to No Test chicks. Experiment 3 involved measurement of behavior in a dust bath apparatus. Contrary to predictions, limited behavior was observed in the apparatus. The second goal of the present study was to be able to reverse the display of anhedonia-like behavior, as measured in the straight alley maze, with pharmaceutical manipulation. Experiment 4 involved administration of vehicle, 10 or 15 mg/kg Imipramine prior to exposure to the isolation apparatus followed by testing in the straight alley maze. Both 10 and 15 mg/kg Imipramine were shown to alleviate the onset of behavioral despair in the isolation test. The 15 mg/kg Imipramine dose also alleviated the display of anhedonia in the straight alley maze. Experiment 5 involved the administration of vehicle, 5 or 10 mg/kg Ketamine prior to exposure to the isolation apparatus followed by testing in the straight alley maze. Neither 5 nor 10 mg/kg Ketamine were able to alleviate the onset of behavioral despair or anhedonia as assessed in the straight alley maze.
Advisors/Committee Members: Michael T. Allen, S. Narasimha Murthy, Michael T. Allen.
Subjects/Keywords: Anhedonia; Animal; Chick; Depression; Model; Psychology
Record Details
Similar Records
Cite
Share »
Record Details
Similar Records
Cite
« Share





❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Salmeto-Johnson, A. L. (2014). Measurement Of Anhedonia In The Chick Anxiety-Depression Model. (Doctoral Dissertation). University of Mississippi. Retrieved from https://egrove.olemiss.edu/etd/838
Chicago Manual of Style (16th Edition):
Salmeto-Johnson, Amy L. “Measurement Of Anhedonia In The Chick Anxiety-Depression Model.” 2014. Doctoral Dissertation, University of Mississippi. Accessed February 28, 2021.
https://egrove.olemiss.edu/etd/838.
MLA Handbook (7th Edition):
Salmeto-Johnson, Amy L. “Measurement Of Anhedonia In The Chick Anxiety-Depression Model.” 2014. Web. 28 Feb 2021.
Vancouver:
Salmeto-Johnson AL. Measurement Of Anhedonia In The Chick Anxiety-Depression Model. [Internet] [Doctoral dissertation]. University of Mississippi; 2014. [cited 2021 Feb 28].
Available from: https://egrove.olemiss.edu/etd/838.
Council of Science Editors:
Salmeto-Johnson AL. Measurement Of Anhedonia In The Chick Anxiety-Depression Model. [Doctoral Dissertation]. University of Mississippi; 2014. Available from: https://egrove.olemiss.edu/etd/838

East Tennessee State University
24.
Hernandez, Liza.
Alcohol Consumption in a Preclinical Model of Schizophrenia.
Degree: MA, Psychology, 2020, East Tennessee State University
URL: https://dc.etsu.edu/etd/3693
► Schizophrenia is a debilitating psychiatric disorder that affects approximately 1% of the global population. Schizophrenia is highly comorbid with other psychiatric disorders such as…
(more)
▼ Schizophrenia is a debilitating psychiatric disorder that affects approximately 1% of the global population. Schizophrenia is highly comorbid with other psychiatric disorders such as Alcohol Use Disorder (AUD) with a prevalence rate of 27% - 65%, which is significantly higher than AUD exhibited by the general population (6%). Research indicates that a higher rate of AUD in individuals suffering from schizophrenia may be related to the common neuronal pathways that underlie the expression of both disorders. The present study will determine whether the neonatal quinpirole (NQ) rodent model of schizophrenia will approximate the human condition and exhibit increased EtOH consumption. Rats will be treated neonatally with quinpirole or saline. Following the treatment period, rats will be tested for EtOH consumption using a 24-hour two-bottle free-access paradigm. The proposed research will test the hypothesis that rats neonatally treated with quinpirole will consume significantly greater amounts of EtOH than their saline counterparts.
Subjects/Keywords: Schizophrenia; Alcohol; Quinpirole; Animal Model; Behavioral Neurobiology
Record Details
Similar Records
Cite
Share »
Record Details
Similar Records
Cite
« Share





❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Hernandez, L. (2020). Alcohol Consumption in a Preclinical Model of Schizophrenia. (Thesis). East Tennessee State University. Retrieved from https://dc.etsu.edu/etd/3693
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Hernandez, Liza. “Alcohol Consumption in a Preclinical Model of Schizophrenia.” 2020. Thesis, East Tennessee State University. Accessed February 28, 2021.
https://dc.etsu.edu/etd/3693.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Hernandez, Liza. “Alcohol Consumption in a Preclinical Model of Schizophrenia.” 2020. Web. 28 Feb 2021.
Vancouver:
Hernandez L. Alcohol Consumption in a Preclinical Model of Schizophrenia. [Internet] [Thesis]. East Tennessee State University; 2020. [cited 2021 Feb 28].
Available from: https://dc.etsu.edu/etd/3693.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Hernandez L. Alcohol Consumption in a Preclinical Model of Schizophrenia. [Thesis]. East Tennessee State University; 2020. Available from: https://dc.etsu.edu/etd/3693
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Colorado State University
25.
Layer, Emily L.
Understanding Mycobacterium abscessus pulmonary and disseminated disease.
Degree: MS(M.S.), Microbiology, Immunology, and Pathology, 2017, Colorado State University
URL: http://hdl.handle.net/10217/183863
► Mycobacterium abscessus is an emerging human pathogen which is difficult to treat and results in increased mortality. Moreover, the cause of increasing case rates and…
(more)
▼ Mycobacterium abscessus is an emerging human pathogen which is difficult to treat and results in increased mortality. Moreover, the cause of increasing case rates and also the pathogenesis of M. abscessus are poorly understood. M. abscessus belongs to the family of nontuberculous mycobacteria (NTM) classified as members of the rapidly growing mycobacteria (RGM). These environmental pathogens are ubiquitous and found in shower heads, tap water, natural water sources, and soil. Humans contract pulmonary or disseminated infections with M. abscessus by breathing in the aerosolized bacteria or ingesting contaminated water. Immunocompromised individuals such as HIV or AIDS patients, are more susceptible to infection with M. abscessus as are those with cystic fibrosis, bronchiectasis, and individuals on tumor necrosis factor α (TNFα) inhibitors. Strangely, an increasing population of patients becoming infected with M. abscessus are immunocompetent, tall, slender, Caucasian, non-smoking women. To expand our understanding of M. abscessus pathogenesis we developed mouse models that maintain high levels of bacterial infection to study immune responses induced by clinical strains of M. abscessus. Our results support the hypothesis that this bacteria can only persist in our
animal models that possess a deficiency in macrophages and T cell function. Clustered bacterial strains obtained from Cystic Fibrosis patients are more virulent than unclustered bacterial strains obtained from Cystic Fibrosis patients. Additionally, counts of viable mycobacterial colony forming units and histological analysis in (Severe Combined Immunodeficiency) SCID mice on a beige background infected with clustered versus unclustered M. abscessus strains which were isolated from Cystic Fibrosis patients also supported the increased virulence exhibited by the clustered strains. Lastly, we show that major human M. abscessus outbreak strains, when infecting IL-3, GM-CSF deficient mice on an IL-2, Rag2 deficient background (GM/Rag-dblKO mice), result in increased bacterial replication and organ pathology and impaired protective immunity against this pathogen.
Advisors/Committee Members: Ordway, Diane (advisor), Orme, Ian (committee member), Chatterjee, Delphi (committee member), Kirby, Michael (committee member).
Subjects/Keywords: animal; mouse; nontuberculous; model; abscessus; mycobacteria
Record Details
Similar Records
Cite
Share »
Record Details
Similar Records
Cite
« Share





❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Layer, E. L. (2017). Understanding Mycobacterium abscessus pulmonary and disseminated disease. (Masters Thesis). Colorado State University. Retrieved from http://hdl.handle.net/10217/183863
Chicago Manual of Style (16th Edition):
Layer, Emily L. “Understanding Mycobacterium abscessus pulmonary and disseminated disease.” 2017. Masters Thesis, Colorado State University. Accessed February 28, 2021.
http://hdl.handle.net/10217/183863.
MLA Handbook (7th Edition):
Layer, Emily L. “Understanding Mycobacterium abscessus pulmonary and disseminated disease.” 2017. Web. 28 Feb 2021.
Vancouver:
Layer EL. Understanding Mycobacterium abscessus pulmonary and disseminated disease. [Internet] [Masters thesis]. Colorado State University; 2017. [cited 2021 Feb 28].
Available from: http://hdl.handle.net/10217/183863.
Council of Science Editors:
Layer EL. Understanding Mycobacterium abscessus pulmonary and disseminated disease. [Masters Thesis]. Colorado State University; 2017. Available from: http://hdl.handle.net/10217/183863

University of Melbourne
26.
Senesi, Matteo.
Behavioural characterization of M1000 prion strain pathogenesis and the acute neurotoxicity of disease-associated prion protein in vivo.
Degree: 2015, University of Melbourne
URL: http://hdl.handle.net/11343/91119
► Prion diseases are a group of transmissible, fatal, neurodegenerative diseases naturally afflicting a number of mammals, including humans and no effective treatments currently exist. The…
(more)
▼ Prion diseases are a group of transmissible, fatal, neurodegenerative diseases naturally afflicting a number of mammals, including humans and no effective treatments currently exist. The most common human prion disease phenotype is Creutzfeldt-Jakob disease (CJD), which is quite rare, occurring at a rate of around 1-2 per million per year, with other forms of human prion disease such as iatrogenic CJD, Gerstmann-Sträussler-Scheinker syndrome (GSS) and fatal familial insomnia (FFI) much less common. Kuru, as well, a prion disease endemic to the Fore linguistic group in the Eastern Highlands of New Guinea, is now most likely extinct stemming from the effective outlawing of cannibalistic mourning rituals amongst these natives. Although rare, concerns around inter- and intra-species transmissibility generally give prion diseases a heightened profile, especially when dealing with health care settings.
The central pathogenic event in prion disease is understood to be the misfolding of the normal or cellular form of the prion protein (PrPc) into a β-strand enriched, disease-causing conformer (PrPSc), which characteristically manifests altered biochemical properties such as detergent insolubility, relative protease resistance and heightened tendency to aggregate. The refolding can be promoted by genetic mutations in the gene encoding PrPc (PRNP) associated with genetic prion disease or occur through a stochastic misfolding event (sporadic prion disease). Once present (including through exogenous introduction), PrPSc propagation proceeds through an auto-catalytic self-templating mechanism, wherein PrPc forms a hetero-dimer with a molecule of PrPSc. The normal function(s) of the glycoprotein PrPc remain unresolved but the protein is ubiquitously expressed with highest levels found in neurons and after a number of post-translational modifications is normally bound through a glycosylphosphatidylinositol anchor to the external cellular surface within lipid rafts. Prions, the infectious unit of prion diseases, are believed to be composed predominantly or exclusively by PrPSc, with host tissue expression of PrPc absolutely essential for successful disease transmission; current evidence suggests that different species of PrPSc may underpin neurotoxicity and transmissibility.
The ongoing imperative to develop effective treatments has been pari passu with investigations to better understand the neurotoxic events and pathogenic mechanisms sub-serving prion disease, with the belief that improved mechanistic insights will facilitate development of effective therapies. Of additional interest has been the recent parallel investigations of other common neurodegenerative diseases, such as Alzheimer’s disease and Parkinson’s disease, which have identified “prion-like” mechanisms of misfolded protein propagation (through self-templating to generate multimers including soluble oligomers and amyloid fibrils), as well as the inter-cellular spreading mechanisms of misfolded proteins. Consequently, an improved understanding of the mechanisms…
Subjects/Keywords: prion; neuroscience; animal model; fear; learning; memory
Record Details
Similar Records
Cite
Share »
Record Details
Similar Records
Cite
« Share





❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Senesi, M. (2015). Behavioural characterization of M1000 prion strain pathogenesis and the acute neurotoxicity of disease-associated prion protein in vivo. (Doctoral Dissertation). University of Melbourne. Retrieved from http://hdl.handle.net/11343/91119
Chicago Manual of Style (16th Edition):
Senesi, Matteo. “Behavioural characterization of M1000 prion strain pathogenesis and the acute neurotoxicity of disease-associated prion protein in vivo.” 2015. Doctoral Dissertation, University of Melbourne. Accessed February 28, 2021.
http://hdl.handle.net/11343/91119.
MLA Handbook (7th Edition):
Senesi, Matteo. “Behavioural characterization of M1000 prion strain pathogenesis and the acute neurotoxicity of disease-associated prion protein in vivo.” 2015. Web. 28 Feb 2021.
Vancouver:
Senesi M. Behavioural characterization of M1000 prion strain pathogenesis and the acute neurotoxicity of disease-associated prion protein in vivo. [Internet] [Doctoral dissertation]. University of Melbourne; 2015. [cited 2021 Feb 28].
Available from: http://hdl.handle.net/11343/91119.
Council of Science Editors:
Senesi M. Behavioural characterization of M1000 prion strain pathogenesis and the acute neurotoxicity of disease-associated prion protein in vivo. [Doctoral Dissertation]. University of Melbourne; 2015. Available from: http://hdl.handle.net/11343/91119

University of Melbourne
27.
Battistuzzo, Camila.
Recovery of locomotion in mice following spinal cord injury.
Degree: 2014, University of Melbourne
URL: http://hdl.handle.net/11343/51133
► Traumatic spinal cord injury (SCI) is a devastating condition with a worldwide incidence of 10 to 40 cases per million people (Norton 2010). In Australia,…
(more)
▼ Traumatic spinal cord injury (SCI) is a devastating condition with a worldwide incidence of 10 to 40 cases per million people (Norton 2010). In Australia, there are approximately 300 to 400 new cases of SCI each year. Over the last 60 years, significant advances in both the acute and rehabilitation management of SCI have resulted in reduced mortality and increased life expectancy. Improvements in the medical care of SCI have also led to an increased proportion of incomplete SCI. These patients generally have some preservation of motor and sensory function; however, achieving functional recovery remains a major challenge for the clinical and research communities.
In the early 1980s, the breakthrough finding that repetitive treadmill training restored stepping in spinal cats had a profound impact on the rehabilitation of people with SCI (Lovely et al. 1986). The focus of rehabilitation changed from training compensation strategies to retraining locomotion using activity-based therapies (e.g. treadmill training). Despite many studies in animal models of complete SCI demonstrating the benefits locomotor training on gait recovery, the evidence for the effects of this type of intervention following incomplete SCI remains unclear. Furthermore, questions about the optimal training dosage and timing are yet to be resolved.
The overall aim of this thesis was to assess the recovery of locomotion in mice with incomplete SCI. An incomplete model involving a lateral hemisection injury was chosen as it was felt that this would allow assessment of recovery between affected and non-affected hindlimbs and be a sensitive method of detecting training effects. In addition to the experiments detailed in this thesis, included animals also had electrophysiological assessments of the spinal cord neurons (not described in this thesis).
The first study of this thesis (Chapter 3) was a systematic review summarizing the evidence of locomotor training after SCI. The results of this review demonstrated that locomotor training in animals after SCI improves locomotion. However, there was significant heterogeneity between studies and the methodological quality of the included studies was often poor. In light of these data, two experimental studies were designed with high methodological standards.
In the second (and first experimental) study (Chapter 4), the effects of treadmill training for 3,6 and 9 weeks on recovery of locomotion were assessed in a mouse model of spinal hemisection. A high-speed camera was used to obtain detailed kinematic gait characteristics of animals with and without training. These data demonstrated that treadmill training had a limited effect on the recovery of gait. Subsequently, (Chapter 5), the effect of treadmill training for 3, 6 and 9 weeks on hindlimb muscle properties in hemisected SCI mice was investigated. The results of this study showed that treadmill training was effective in preventing atrophy of fast-twitch muscles (e.g. tibialis anterior) but had limited effects on slow-twitch muscles (e.g.…
Subjects/Keywords: spinal cord injury; exercise; animal model
Record Details
Similar Records
Cite
Share »
Record Details
Similar Records
Cite
« Share





❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Battistuzzo, C. (2014). Recovery of locomotion in mice following spinal cord injury. (Doctoral Dissertation). University of Melbourne. Retrieved from http://hdl.handle.net/11343/51133
Chicago Manual of Style (16th Edition):
Battistuzzo, Camila. “Recovery of locomotion in mice following spinal cord injury.” 2014. Doctoral Dissertation, University of Melbourne. Accessed February 28, 2021.
http://hdl.handle.net/11343/51133.
MLA Handbook (7th Edition):
Battistuzzo, Camila. “Recovery of locomotion in mice following spinal cord injury.” 2014. Web. 28 Feb 2021.
Vancouver:
Battistuzzo C. Recovery of locomotion in mice following spinal cord injury. [Internet] [Doctoral dissertation]. University of Melbourne; 2014. [cited 2021 Feb 28].
Available from: http://hdl.handle.net/11343/51133.
Council of Science Editors:
Battistuzzo C. Recovery of locomotion in mice following spinal cord injury. [Doctoral Dissertation]. University of Melbourne; 2014. Available from: http://hdl.handle.net/11343/51133

University of Melbourne
28.
Krenus, Charlotte.
Models of spontaneous intracerebral haemorrhage in hypertensive rats.
Degree: 2017, University of Melbourne
URL: http://hdl.handle.net/11343/210712
► Few animal models of stroke have created haematomas that mimic the processes seen in humans. We developed a new model of intracerebral haemorrhage (ICH) in…
(more)
▼ Few animal models of stroke have created haematomas that mimic the processes seen in humans. We developed a new model of intracerebral haemorrhage (ICH) in rats by inducing acute-on-chronic hypertension that more closely mimics the human condition than existing models. We also examined profiles of ICH development in the genetically hypertensive Spontaneously Hypertensive Rat and in normotensive rats made hypertensive by induction of renal disease via renal artery obstruction or kidney resection.
Subjects/Keywords: intracerebral haemorrhage; stroke; hypertension; animal model; translation
Record Details
Similar Records
Cite
Share »
Record Details
Similar Records
Cite
« Share





❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Krenus, C. (2017). Models of spontaneous intracerebral haemorrhage in hypertensive rats. (Doctoral Dissertation). University of Melbourne. Retrieved from http://hdl.handle.net/11343/210712
Chicago Manual of Style (16th Edition):
Krenus, Charlotte. “Models of spontaneous intracerebral haemorrhage in hypertensive rats.” 2017. Doctoral Dissertation, University of Melbourne. Accessed February 28, 2021.
http://hdl.handle.net/11343/210712.
MLA Handbook (7th Edition):
Krenus, Charlotte. “Models of spontaneous intracerebral haemorrhage in hypertensive rats.” 2017. Web. 28 Feb 2021.
Vancouver:
Krenus C. Models of spontaneous intracerebral haemorrhage in hypertensive rats. [Internet] [Doctoral dissertation]. University of Melbourne; 2017. [cited 2021 Feb 28].
Available from: http://hdl.handle.net/11343/210712.
Council of Science Editors:
Krenus C. Models of spontaneous intracerebral haemorrhage in hypertensive rats. [Doctoral Dissertation]. University of Melbourne; 2017. Available from: http://hdl.handle.net/11343/210712

University of Hawaii – Manoa
29.
Ciraolo, Margeaux Faye.
A computational investigation of relational reasoning in nonhuman animals.
Degree: 2016, University of Hawaii – Manoa
URL: http://hdl.handle.net/10125/101253
► M.A. University of Hawaii at Manoa 2012.
Since Darwin (1859) suggested that all animals share a common ancestry, researchers have attempted to assess how the…
(more)
▼ M.A. University of Hawaii at Manoa 2012.
Since Darwin (1859) suggested that all animals share a common ancestry, researchers have attempted to assess how the divergence between human and nonhuman animal biology and cognition came about. Animals have a large repertoire of physical and behavioral adaptations that have afforded them the ability to overcome myriad obstacles to their existence. On the behavioral level, these adaptations are as diverse as the species that exhibit them. One merely has to select an animal species at random and do a cursory investigation in order to find many complex behaviors which make that animal a perfect fit for the environment that it inhabits. However, it is possible that the high degree to which an animal is adapted to its environment may limit that animal's ability to employ flexible behavior. For example, the digger wasp has a highly stereotypical set of behavioral steps for readying a nest. If any one of these steps is disrupted in the process of nest creation, the digger wasp will not alter its behavioral program, even if doing so would ensure the life of its offspring (Fabre, 1919). In short, the digger wasp lacks flexibility in its behavioral routine. Indeed, many animal species display rigidity in their behavior. Humans, on the other hand, appear to have much more control of their behavior as well as a flexibility that has allowed them to manipulate and change their environment. The mechanism that underlies this ability may be at the heart of what makes human and nonhuman animal cognition so qualitatively different.
Subjects/Keywords: cognition; animal cognition; computational model; relational reasoning
Record Details
Similar Records
Cite
Share »
Record Details
Similar Records
Cite
« Share





❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Ciraolo, M. F. (2016). A computational investigation of relational reasoning in nonhuman animals. (Thesis). University of Hawaii – Manoa. Retrieved from http://hdl.handle.net/10125/101253
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Ciraolo, Margeaux Faye. “A computational investigation of relational reasoning in nonhuman animals.” 2016. Thesis, University of Hawaii – Manoa. Accessed February 28, 2021.
http://hdl.handle.net/10125/101253.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Ciraolo, Margeaux Faye. “A computational investigation of relational reasoning in nonhuman animals.” 2016. Web. 28 Feb 2021.
Vancouver:
Ciraolo MF. A computational investigation of relational reasoning in nonhuman animals. [Internet] [Thesis]. University of Hawaii – Manoa; 2016. [cited 2021 Feb 28].
Available from: http://hdl.handle.net/10125/101253.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Ciraolo MF. A computational investigation of relational reasoning in nonhuman animals. [Thesis]. University of Hawaii – Manoa; 2016. Available from: http://hdl.handle.net/10125/101253
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
30.
Siemienowicz, Katarzyna Joanna.
Fetal programming of adult disease : causes and consequences of metabolic dysregulation in an ovine model of PCOS.
Degree: PhD, 2018, University of Edinburgh
URL: http://hdl.handle.net/1842/28977
► Polycystic ovary syndrome (PCOS) is a common and complex endocrine condition with reproductive and metabolic complications, affecting up to 10% of reproductive-age women. Hyperandrogenemia, ovulatory…
(more)
▼ Polycystic ovary syndrome (PCOS) is a common and complex endocrine condition with reproductive and metabolic complications, affecting up to 10% of reproductive-age women. Hyperandrogenemia, ovulatory dysfunction, and luteinising hormone hypersecretion are characteristic traits of PCOS however, it seems that the most concerning long-term key issues are metabolic problems associated with the syndrome, such as hyperinsulinemia, insulin resistance, obesity, dyslipidaemia and non-alcoholic liver disease. Despite the numerous studies on PCOS, its origin and pathophysiology are still not fully understood. However, there is increasing evidence that the adult PCOS phenotype is programmed in fetal life by androgen excess. Exposure to increased levels of testosterone in utero in rodents, sheep and monkeys result in adult reproductive and metabolic pathologies that parallel those seen in PCOS women. Since hyperandrogenemia is a hallmark of PCOS and daughters of PCOS mothers have elevated levels of androgens at birth, it is likely that prenatal androgenisation during early life predispose to the future development of PCOS. Animal models of PCOS provide an opportunity to examine the developmental aetiology and molecular mechanisms underlying the pathogenesis of this condition. Over last 10 years our lab has successfully utilised a well-established ovine model of PCOS, where pregnant ewes were treated with testosterone propionate (TP) through mid-gestation. From this model, we had a large sample bank of fixed and frozen tissues from the fetal, lamb and adolescent prenatally androgenised animals that allowed to carry a broad range of experiments. In addition, a new cohort of prenatally androgenised adult sheep enabled additional in vivo analysis. Past research documented that prenatal androgenisation result in hyperinsulinemia with altered pancreas structure and function, and early fatty liver without difference in body weight in adolescent sheep. This thesis examines the effects and consequences of increased in utero androgen exposure on metabolic dysregulation in adolescent and adult female sheep. During puberty, but not fetal or early life, there was decreased adipogenesis in subcutaneous adipose tissue (SAT), but not visceral adipose tissue (VAT), accompanied by decreased circulating concentrations of fibroblast growth factor 21 (FGF21), leptin and adiponectin, and increased concentrations of fasting free fatty acids (FFA) in prenatally androgenised sheep. This was countered by upregulated expression of FFA transporters in liver. As adults, TP-exposed animals had increased body weight, elevated fasting insulin and FFA concentrations but normal FGF21, leptin and adiponectin levels. Histological analysis revealed that adult TP-exposed animals had SAT hypertrophy, which was associated with increased expression of inflammatory markers and correlated with increased fasting FFA. Therefore, it is likely that impaired preadipocyte differentiation in SAT during adolescence resulted in hypertrophy and inflammation of adult SAT. This…
Subjects/Keywords: polycystic ovary syndrome; PCOS; androgen; animal model
Record Details
Similar Records
Cite
Share »
Record Details
Similar Records
Cite
« Share





❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Siemienowicz, K. J. (2018). Fetal programming of adult disease : causes and consequences of metabolic dysregulation in an ovine model of PCOS. (Doctoral Dissertation). University of Edinburgh. Retrieved from http://hdl.handle.net/1842/28977
Chicago Manual of Style (16th Edition):
Siemienowicz, Katarzyna Joanna. “Fetal programming of adult disease : causes and consequences of metabolic dysregulation in an ovine model of PCOS.” 2018. Doctoral Dissertation, University of Edinburgh. Accessed February 28, 2021.
http://hdl.handle.net/1842/28977.
MLA Handbook (7th Edition):
Siemienowicz, Katarzyna Joanna. “Fetal programming of adult disease : causes and consequences of metabolic dysregulation in an ovine model of PCOS.” 2018. Web. 28 Feb 2021.
Vancouver:
Siemienowicz KJ. Fetal programming of adult disease : causes and consequences of metabolic dysregulation in an ovine model of PCOS. [Internet] [Doctoral dissertation]. University of Edinburgh; 2018. [cited 2021 Feb 28].
Available from: http://hdl.handle.net/1842/28977.
Council of Science Editors:
Siemienowicz KJ. Fetal programming of adult disease : causes and consequences of metabolic dysregulation in an ovine model of PCOS. [Doctoral Dissertation]. University of Edinburgh; 2018. Available from: http://hdl.handle.net/1842/28977
◁ [1] [2] [3] [4] [5] … [24] ▶
.