subject:(Mod les in vitro)

1. Lemoine, Manuela. La réaction acrosomique du spermatozoïde chez le coq : The chicken acrosome reaction.

Degree: Docteur es, Sciences de la vie, 2009, Université François-Rabelais de Tours

URL: http://www.theses.fr/2009TOUR4005

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L’objectif de la thèse a été d’apporter des éléments sur la réaction acrosomique (RA) aviaire afin de mieux comprendre les processus menant à la fécondation… (more)

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L’objectif de la thèse a été d’apporter des éléments sur la réaction acrosomique (RA) aviaire afin de mieux comprendre les processus menant à la fécondation et de mieux maîtriser la capacité des spermatozoïdes à être conservés. Nos résultats ont conforté l’hypothèse de l’absence de capacitation chez les oiseaux. De plus, il n’y a pas d’hyperactivation de la mobilité lors de la RA. Seul le Ca2+ s’avère être l’élément indispensable au déclenchement de la RA. L’évaluation de la RA avec des spermatozoïdes conservés à l’état liquide ou après cryoconservation a révélé une évolution différente en fonction du type de conservation. L’étude des voies de signalisation susceptibles d’être impliquées dans le déclenchement de la RA a suggéré l’activation de 3voies, PKA, PI3K et MAPK ERK. Ce travail ouvre de nombreuses perspectives scientifiques vers l’approfondissement des connaissances de la RA chez les oiseaux et sur l’utilisation qui peut en être faite pour mieux maîtriser la qualité des gamètes.

The aim of this work was to provide new information on chicken acrosome reaction (AR) for a better comprehension of the mechanisms leading to this reaction and a better control of the fertilizing potential of spermatozoa after in vitro storage. Our results showed that calcium is the factor absolutely necessary to initiate the AR and supported the hypothesis that chicken spermatozoa do not need to be capacitated. Moreover, motility hyperactivation was not found at the time of AR. Then, we showed that chicken sperm ability to undergo the AR may differ depending on the type of semen storage. Indeed, this ability was dramatically affected by liquid storage, but was submitted to contrasted effect after cryopreservation. Finally, we investigated the potential involvement of several signaling pathways in initiation of the chicken AR and the results showed that the AR could be mediated by activation of the PKA, PI3K and ERK MAPK pathways.

Advisors/Committee Members: Blesbois, Elisabeth (thesis director).

Subjects/Keywords: Conservation in vitro

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APA (6^th Edition):

Lemoine, M. (2009). La réaction acrosomique du spermatozoïde chez le coq : The chicken acrosome reaction. (Doctoral Dissertation). Université François-Rabelais de Tours. Retrieved from http://www.theses.fr/2009TOUR4005

Chicago Manual of Style (16^th Edition):

Lemoine, Manuela. “La réaction acrosomique du spermatozoïde chez le coq : The chicken acrosome reaction.” 2009. Doctoral Dissertation, Université François-Rabelais de Tours. Accessed December 06, 2019. http://www.theses.fr/2009TOUR4005.

MLA Handbook (7^th Edition):

Lemoine, Manuela. “La réaction acrosomique du spermatozoïde chez le coq : The chicken acrosome reaction.” 2009. Web. 06 Dec 2019.

Vancouver:

Lemoine M. La réaction acrosomique du spermatozoïde chez le coq : The chicken acrosome reaction. [Internet] [Doctoral dissertation]. Université François-Rabelais de Tours; 2009. [cited 2019 Dec 06]. Available from: http://www.theses.fr/2009TOUR4005.

Council of Science Editors:

Lemoine M. La réaction acrosomique du spermatozoïde chez le coq : The chicken acrosome reaction. [Doctoral Dissertation]. Université François-Rabelais de Tours; 2009. Available from: http://www.theses.fr/2009TOUR4005

2. Fuster, Laura. Mise en oeuvre d’une démarche intégrée pour identifier des contaminants pertinents dans l’environnement : An Integrated approach for identifying contaminants of concern in environment.

Degree: Docteur es, Chimie analytique et environnementale, 2017, Bordeaux

URL: http://www.theses.fr/2017BORD0880

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De nos jours, l’évaluation de la qualité d’un milieu naturel repose traditionnellement sur des analyses ciblant des molécules déjà connues pour être biologiquement actives. Cependant… (more)

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De nos jours, l’évaluation de la qualité d’un milieu naturel repose traditionnellement sur des analyses ciblant des molécules déjà connues pour être biologiquement actives. Cependant ces molécules ne reflètent que partiellement les effets biologiques observés et la complexité des matrices environnementales. Par ailleurs, les contaminants dans l’environnement subissent des phénomènes de dégradation (hydrolyse, biodégradation, photodégradation). Ces processus peuvent conduire à la génération de produits de transformation qui sont encore trop peu pris en compte dans l’évaluation du risque, alors qu’ils sont susceptibles d’occasionner des effets toxiques plus importants que les molécules mères. L’impact écotoxicologique de ces mélanges nécessite préalablement l’obtention d’une vision globale quant à la présence et au devenir de ces molécules.Dans ce contexte, ces travaux appliquent une méthodologie pluridisciplinaire afin d’identifier des molécules préoccupantes et pertinentes à surveiller. Pour cela, ce travail s’appuie sur des analyses ciblées ultra-traces, des outils bioanalytiques, et des analyses non ciblées, qui ont été appliqués sur des échantillons environnementaux ou issus d’études en laboratoire mimant l’évolution des matrices environnementales. Au final, cette étude a permis : (1) d’identifier des molécules préoccupantes en estuaire de Seine sur la base de leur occurrence et de leur persistance, (2) d’identifier de nouveaux produits de transformation et (3) d’identifier les molécules responsables d’activités biologiques dans des matrices très complexes.

To date, environmental risk assessment is based on a restricted number of molecules assessed by targeted chemical analyses. However, this approach give a partial picture of co-occurrence of known and unknown compounds. Moreover, in the aquatic environment, chemicals are not completely mineralized and are subject to abiotic and biotic processes. Transformation products (TPs) can be more toxic and more persistent than the parent compound. However, TPs are not typically included in classical monitoring and risk assessment. Because of complexity and variability of these matrices and the restricted number of molecules focused in targeted chemical analyses, selection of relevant molecules for environmental monitoring is often laborious.In this context, an integrative approach was used in order to identify chemicals of concern for a classical monitoring. This work has been realized on environmental complex samples and on laboratory-generated samples. A combination of targeted, non-targeted analyses and in vitro bioassays was performed and allowed to: (1) identify polar chemical of concern in the Seine estuary on the basis of occurrence and persistence, (2) identify new transformation products and (3) identify compounds responsible of biological activity observed in complexes matrices.

Advisors/Committee Members: Budzinski, Hélène (thesis director), Mazellier, Patrick (thesis director).

Subjects/Keywords: Contaminants émergents; Produits de transformation; Analyse dirigée par les effets (EDA); Spectrométrie de masse haute résolution; Tests in vitro; Évaluation du risque; Emerging contaminants; Transformation products; Effect-Directed Analysis (EDA); High resolution mass spectrometry; In vitro bioassays; Risk assessment

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Fuster, L. (2017). Mise en oeuvre d’une démarche intégrée pour identifier des contaminants pertinents dans l’environnement : An Integrated approach for identifying contaminants of concern in environment. (Doctoral Dissertation). Bordeaux. Retrieved from http://www.theses.fr/2017BORD0880

Chicago Manual of Style (16^th Edition):

Fuster, Laura. “Mise en oeuvre d’une démarche intégrée pour identifier des contaminants pertinents dans l’environnement : An Integrated approach for identifying contaminants of concern in environment.” 2017. Doctoral Dissertation, Bordeaux. Accessed December 06, 2019. http://www.theses.fr/2017BORD0880.

MLA Handbook (7^th Edition):

Fuster, Laura. “Mise en oeuvre d’une démarche intégrée pour identifier des contaminants pertinents dans l’environnement : An Integrated approach for identifying contaminants of concern in environment.” 2017. Web. 06 Dec 2019.

Vancouver:

Fuster L. Mise en oeuvre d’une démarche intégrée pour identifier des contaminants pertinents dans l’environnement : An Integrated approach for identifying contaminants of concern in environment. [Internet] [Doctoral dissertation]. Bordeaux; 2017. [cited 2019 Dec 06]. Available from: http://www.theses.fr/2017BORD0880.

Council of Science Editors:

Fuster L. Mise en oeuvre d’une démarche intégrée pour identifier des contaminants pertinents dans l’environnement : An Integrated approach for identifying contaminants of concern in environment. [Doctoral Dissertation]. Bordeaux; 2017. Available from: http://www.theses.fr/2017BORD0880

3. Laborie, Stéphanie. Exposition humaine aux perturbateurs endocriniens par inhalation : caractérisation de la contamination de l’air intérieur par analyses chimiques et biologiques in vitro : Human exposure to endocrine disruptors by inhalation : characterization of indoor air contamination by chemical and in vitro biological analyses.

Degree: Docteur es, Chimie, Environnement et Santé, 2015, Paris, EPHE

URL: http://www.theses.fr/2015EPHE3059

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L’objectif de ce projet a été de développer une approche bio-analytique permettant l’évaluation du danger inhérent à la multi-contamination de l’air intérieur en Ile-de-France. Des… (more)

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L’objectif de ce projet a été de développer une approche bio-analytique permettant l’évaluation du danger inhérent à la multi-contamination de l’air intérieur en Ile-de-France. Des méthodes d’analyse chromatographique couplée à la spectrométrie de masse ont été développées et validées pour 62 composés d’intérêt présentant un potentiel perturbateur endocrinien (PE) avéré ou suspecté. Le potentiel PE a été évalué sur des bio-essais cellulaires de mesure de perturbation d’activité transcriptionnelle. Les résultats montrent que les familles de composés majoritaires dans l’air intérieur sont, par ordre décroissant : phtalates > muscs synthétiques > alkylphénols > parabènes. En outre, les composés sont prédominants en phase gazeuse, et les habitats les plus contaminés sont la crèche et la maison. L’air intérieur présente un potentiel PE de type œstrogénique, thyroïdien et anti-androgénique. En accord avec son profil de contamination, l’activité biologique de ce dernier se concentre majoritairement dans la phase gazeuse, et tend à être plus élevée dans la crèche et la maison. Une analyse dirigée par les bio-essais, ou effect-directed analysis (EDA), a été mise en œuvre pour identifier les composés cibles à l’origine des effets PE de l’air intérieur. Les composés suivants ont été identifiés comme étant potentiellement à l’origine d’effets PE observés : les phtalates, le méthyl-parabène, les alkylphénols, la cyperméthrine et les muscs synthétiques. Ce travail apporte des connaissances sur le danger inhérent à la multi-contamination de l’air intérieur ainsi que des données d’exposition utiles à une évaluation des risques sanitaires.

The objective of this project was to develop a bio-analytical approach leading to the assessment of the inherent hazard of the indoor air multi-contamination. Chromatographic methods combined with mass spectrometry were developed and validated for 62 target molecules known or suspected as endocrine-disrupting (ED) compounds. The ED potential was assessed by cellular bioassays measuring perturbations of transcriptional activity. The data showed that the predominant families of compounds in indoor air were in the following descendant order: phthalates > musks > alkylphenols > parabens. The ED contaminants were mainly present in gaseous phase, and the most contaminated locations were the day nursery and the house. An estrogenic, thyroid and anti-androgenic potential was attributed to indoor air. In agreement with its contamination profile, the biological activity of the latter was concentrated predominantly in the gaseous phase, and tended to be higher in the day nursery and the house. An effect-directed analysis (EDA) was carried out to identify the target chemicals responsible for the ED effects of indoor air. The following chemicals were identified as being potentially responsible for the observed ED effects: phthalates, methyl-paraben, alkylphenols, cypermethrin and synthetic musks. This work provides both knowledge about the inherent hazard of the indoor air multi-contamination and exposure…

Advisors/Committee Members: Chevreuil, Marc (thesis director).

Subjects/Keywords: Analyse biologique in vitro; Perturbateurs endocriniens; Analyse chromatographique; Analyse dirigée par les bio-essais; Air intérieur; Spectrométrie de masse; In vitro biological assay; Endocrine disruptors; Chromatographic analysis; Effect-directed analysis; Indoor air; Mass spectrometry

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Laborie, S. (2015). Exposition humaine aux perturbateurs endocriniens par inhalation : caractérisation de la contamination de l’air intérieur par analyses chimiques et biologiques in vitro : Human exposure to endocrine disruptors by inhalation : characterization of indoor air contamination by chemical and in vitro biological analyses. (Doctoral Dissertation). Paris, EPHE. Retrieved from http://www.theses.fr/2015EPHE3059

Chicago Manual of Style (16^th Edition):

Laborie, Stéphanie. “Exposition humaine aux perturbateurs endocriniens par inhalation : caractérisation de la contamination de l’air intérieur par analyses chimiques et biologiques in vitro : Human exposure to endocrine disruptors by inhalation : characterization of indoor air contamination by chemical and in vitro biological analyses.” 2015. Doctoral Dissertation, Paris, EPHE. Accessed December 06, 2019. http://www.theses.fr/2015EPHE3059.

MLA Handbook (7^th Edition):

Laborie, Stéphanie. “Exposition humaine aux perturbateurs endocriniens par inhalation : caractérisation de la contamination de l’air intérieur par analyses chimiques et biologiques in vitro : Human exposure to endocrine disruptors by inhalation : characterization of indoor air contamination by chemical and in vitro biological analyses.” 2015. Web. 06 Dec 2019.

Vancouver:

Laborie S. Exposition humaine aux perturbateurs endocriniens par inhalation : caractérisation de la contamination de l’air intérieur par analyses chimiques et biologiques in vitro : Human exposure to endocrine disruptors by inhalation : characterization of indoor air contamination by chemical and in vitro biological analyses. [Internet] [Doctoral dissertation]. Paris, EPHE; 2015. [cited 2019 Dec 06]. Available from: http://www.theses.fr/2015EPHE3059.

Council of Science Editors:

Laborie S. Exposition humaine aux perturbateurs endocriniens par inhalation : caractérisation de la contamination de l’air intérieur par analyses chimiques et biologiques in vitro : Human exposure to endocrine disruptors by inhalation : characterization of indoor air contamination by chemical and in vitro biological analyses. [Doctoral Dissertation]. Paris, EPHE; 2015. Available from: http://www.theses.fr/2015EPHE3059

4. Clouet, Elodie. Intégration des modèles in vitro dans la stratégie d'évaluation de la sensibilisation cutanée : Integration of in vitro models in risk assessment of skin sensitization.

Degree: Docteur es, Toxicologie, 2018, Paris Saclay

URL: http://www.theses.fr/2018SACLS003

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Résumé : Depuis l'interdiction en 2013 des tests sur les animaux par le Règlement cosmétique n°1223/2009, différentes méthodes in vitro ont été développées. Toutefois, selon… (more)

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Résumé : Depuis l'interdiction en 2013 des tests sur les animaux par le Règlement cosmétique n°1223/2009, différentes méthodes in vitro ont été développées. Toutefois, selon un consensus scientifique, aucune méthode ne peut couvrir à elle seule l’ensemble des événements clés (KE) définis pour la sensibilisation cutanée.Après un état de l’art des méthodes alternatives relatives à la sensibilisation cutanée, nous avons sélectionné et comparé 3 tests pour ensuite déterminer la meilleure stratégie à suivre. Dans le but de proposer un nouveau test intégré, nous avons adressé l’ensemble des KEs au sein d’un même type cellulaire. La cellule dendritique (DC) jouant un rôle clé dans le développement de la dermatite de contact allergique (DCA), notre choix s’est porté sur la lignée humaine pro-monocytaire THP-1. Nous avons étudié comme événements initiaux (KE1) les formes réactives à l’oxygène (FRO) et le glutathion (GSH), la voie Nrf2-Keap1 (voie centrale de détoxication) et l’expression génique pour le KE2, ainsi que les modifications phénotypiques pour le KE3.Nous avons montré que les allergisants forts induisent une production précoce des FRO associée à une réduction du GSH. Ils activent également la voie Nrf2-Keap1 et induisent l’expression des marqueurs de surface cellulaire CD54 et CD86, ainsi qu’une production de cytokines spécifiques (IL-8, IL-18,...).Pour conclure, ce travail a permis de proposer un test intégrant l’ensemble des mesures biologiques comme différents KE au sein d’un même type cellulaire.

Abstract : Since the animal testing ban in 2013 by Cosmetics Regulation n°1223/2009, various in vitro methods have been developed. However, according to a scientific consensus, no single method can stand-alone to cover the different key events (KE) defined for skin sensitization.After a state of the art of alternative methods relating to skin sensitization, we selected and compared 3 tests to determine the best strategy to follow. In order to propose a new integrated test, we wanted to address all KE within the same cell line. Because dendritic cell (DC) plays a key role in the development of allergic contact dermatitis (ACD), we have chosen the pro-monocytic human line THP-1. We have studied as initial events (KE1), reactive oxygen species (ROS) and glutathione (GSH), Nrf2-Keap1 pathway (central detoxification pathway) and gene expression for KE2 as well as phenotypic modifications for KE3.We have shown that strong allergens are correlated with early production of FRO associated with GSH reduction. They also activate the Nrf2-Keap1 pathway and induce the expression of CD54 and CD86 cell surface markers as well as production of specific cytokines (IL-8, IL-18, etc.).To conclude, this work propose a new assay integrating all the biological measures as different KEs within the same cell.

Advisors/Committee Members: Kerdine-Rômer, Saadia (thesis director), Ferret, Pierre-Jacques (thesis director).

Subjects/Keywords: Sensibilisation cutanée; Stratégie de tests intégrés; Voie toxicologique impliquée dans les effets indésirables; Thp-1; In vitro; Nrf2; Skin sensitization; Integrated testing strategy; Adverse outcome pathway (AOP); Thp-1; In vitro; Nrf2

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Clouet, E. (2018). Intégration des modèles in vitro dans la stratégie d'évaluation de la sensibilisation cutanée : Integration of in vitro models in risk assessment of skin sensitization. (Doctoral Dissertation). Paris Saclay. Retrieved from http://www.theses.fr/2018SACLS003

Chicago Manual of Style (16^th Edition):

Clouet, Elodie. “Intégration des modèles in vitro dans la stratégie d'évaluation de la sensibilisation cutanée : Integration of in vitro models in risk assessment of skin sensitization.” 2018. Doctoral Dissertation, Paris Saclay. Accessed December 06, 2019. http://www.theses.fr/2018SACLS003.

MLA Handbook (7^th Edition):

Clouet, Elodie. “Intégration des modèles in vitro dans la stratégie d'évaluation de la sensibilisation cutanée : Integration of in vitro models in risk assessment of skin sensitization.” 2018. Web. 06 Dec 2019.

Vancouver:

Clouet E. Intégration des modèles in vitro dans la stratégie d'évaluation de la sensibilisation cutanée : Integration of in vitro models in risk assessment of skin sensitization. [Internet] [Doctoral dissertation]. Paris Saclay; 2018. [cited 2019 Dec 06]. Available from: http://www.theses.fr/2018SACLS003.

Council of Science Editors:

Clouet E. Intégration des modèles in vitro dans la stratégie d'évaluation de la sensibilisation cutanée : Integration of in vitro models in risk assessment of skin sensitization. [Doctoral Dissertation]. Paris Saclay; 2018. Available from: http://www.theses.fr/2018SACLS003

5. Mansseri née Lamrioui, Akila. Etude de la multiplicatoin par clonage "in vitro" du merisier (Prunus avium L.).

Degree: 2011, Université M'Hamed Bougara Boumerdès

URL: http://dlibrary.univ-boumerdes.dz:8080/handle/123456789/103

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161 p. , ill. , 30 cm

Le présent travail a porté sur l’étude de la multiplication du Merisier (Prunus avium L.) par clonage in… (more)

Subjects/Keywords: Clonage; Multiplication in vitro; Merisier

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APA (6^th Edition):

Mansseri née Lamrioui, A. (2011). Etude de la multiplicatoin par clonage "in vitro" du merisier (Prunus avium L.). (Thesis). Université M'Hamed Bougara Boumerdès. Retrieved from http://dlibrary.univ-boumerdes.dz:8080/handle/123456789/103

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Mansseri née Lamrioui, Akila. “Etude de la multiplicatoin par clonage "in vitro" du merisier (Prunus avium L.).” 2011. Thesis, Université M'Hamed Bougara Boumerdès. Accessed December 06, 2019. http://dlibrary.univ-boumerdes.dz:8080/handle/123456789/103.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Mansseri née Lamrioui, Akila. “Etude de la multiplicatoin par clonage "in vitro" du merisier (Prunus avium L.).” 2011. Web. 06 Dec 2019.

Vancouver:

Mansseri née Lamrioui A. Etude de la multiplicatoin par clonage "in vitro" du merisier (Prunus avium L.). [Internet] [Thesis]. Université M'Hamed Bougara Boumerdès; 2011. [cited 2019 Dec 06]. Available from: http://dlibrary.univ-boumerdes.dz:8080/handle/123456789/103.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Mansseri née Lamrioui A. Etude de la multiplicatoin par clonage "in vitro" du merisier (Prunus avium L.). [Thesis]. Université M'Hamed Bougara Boumerdès; 2011. Available from: http://dlibrary.univ-boumerdes.dz:8080/handle/123456789/103

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Georgia

6. Clark, Damian Reynard. Improving in vitro protein digestibility and determining protein nutritional quality by modelling.

Degree: MS, Food Science, 2004, University of Georgia

URL: http://purl.galileo.usg.edu/uga_etd/clark_damian_r_200405_ms

► Casein, sorghum, cowpea, extruded sorghum, and extruded cowpea samples were treated with bile salts and solublized and non-solublized fractions were separated by TCA precipitation. Digested… (more)

Subjects/Keywords: In Vitro

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APA (6^th Edition):

Clark, D. R. (2004). Improving in vitro protein digestibility and determining protein nutritional quality by modelling. (Masters Thesis). University of Georgia. Retrieved from http://purl.galileo.usg.edu/uga_etd/clark_damian_r_200405_ms

Chicago Manual of Style (16^th Edition):

Clark, Damian Reynard. “Improving in vitro protein digestibility and determining protein nutritional quality by modelling.” 2004. Masters Thesis, University of Georgia. Accessed December 06, 2019. http://purl.galileo.usg.edu/uga_etd/clark_damian_r_200405_ms.

MLA Handbook (7^th Edition):

Clark, Damian Reynard. “Improving in vitro protein digestibility and determining protein nutritional quality by modelling.” 2004. Web. 06 Dec 2019.

Vancouver:

Clark DR. Improving in vitro protein digestibility and determining protein nutritional quality by modelling. [Internet] [Masters thesis]. University of Georgia; 2004. [cited 2019 Dec 06]. Available from: http://purl.galileo.usg.edu/uga_etd/clark_damian_r_200405_ms.

Council of Science Editors:

Clark DR. Improving in vitro protein digestibility and determining protein nutritional quality by modelling. [Masters Thesis]. University of Georgia; 2004. Available from: http://purl.galileo.usg.edu/uga_etd/clark_damian_r_200405_ms

Universidade Estadual de Campinas

7. Klimesch,Yvonne, 1978-. Avaliação da imunoterapia com linfócitos paternos em casais com falhas de implantação após fertilização in vitro = The influence of immunotherapy with paternal lymphocytes in the results of in vitro fertilization in couples with implantation failures .

Degree: 2014, Universidade Estadual de Campinas

URL: http://repositorio.unicamp.br/jspui/handle/REPOSIP/313018

► Resumo: Introdução: As altas taxas de falha de implantação embrionária vêm sendo uma das principais causas no insucesso dos ciclos de fertilização in vitro. A… (more)

▼ Resumo: Introdução: As altas taxas de falha de implantação embrionária vêm sendo uma das principais causas no insucesso dos ciclos de fertilização in vitro. A incompatibilidade imunológica entre o embrião e a decídua materna gera uma ineficiência na produção de anticorpos bloqueadores com um aumento no predomínio de citocinas do tipo Th1 que são prejudiciais ao processo da gestação. A imunoterapia com linfócitos paternos surge como tratamento adjuvante com a função de reverter essa alteração imunológica para um predomínio do tipo Th2 favorecendo assim a tolerância imunológica permitindo a implantação embrionária e posterior desenvolvimento gestacional aumentando as taxas nos ciclos de fertilização assistida. Objetivos: Avaliar o tratamento de imunização com linfócitos paternos, os resultados maternos fetais e perinatais dos casais que tiveram falhas de implantação em ciclos de fertilização in vitro (FIV/ICSI). Materiais e métodos: Através de busca ativa pelos prontuários médicos foram incluídos no estudo 52 casais que apresentavam pelo menos duas falhas nos ciclos de fertilização previamente a imunoterapia. A imunização com linfócitos paternos foi administrada via intradérmica a cada 21 dias, totalizando 3 doses. Após 3 semanas da última dose realizou-se o teste de crossmatch, cujo percentual aceito é superior a 75% para ser considerado positivo e assim indicando a sensibilização da paciente com antígenos de histocompatibilidade do esposo. Resultados: Dos 52 casais incluídos no estudo, 20 casais não conseguiram uma gestação e 32 casais conseguiram uma gestação. No grupo que engravidou, a taxa de gravidez clínica foi de 61,5% (32/52) e a taxa de gravidez viável foi de 57,7% (30/52). Das 32 gestações, 30 evoluíram para parto e duas evoluíram para aborto de primeiro trimestre. A taxa de prematuridade foi de 53%. Conclusão: A imunoterapia com linfócitos paternos pode aumentar as taxas de gestação em mulheres que tiveram falhas prévias nos ciclos de fertilização assistida; Abstract: Introduction: High rates of embryo implantation failures have been a major cause of failure in IVF cycles. The immunological incompatibility between the embryo and the maternal decidua produces inefficiency in the production of blocking antibodies with an increase in the predominance of Th1-type cytokines that are detrimental to the process of gestation. Immunotherapy with paternal lymphocytes appears as adjuvant treatment with immune function reverse this change to a predominance of Th2 thus promoting immune tolerance allowing embryonic development and subsequent gestational increasing implantation rates in assisted fertilization cycles.Objective: To evaluate the treatment of immunization with paternal lymphocytes, fetal and perinatal maternal outcomes of couples who had implantation failure in in vitro fertilization cycles (IVF / ICSI). Material and Methods: Through active search for medical records were included in the study 52 couples with at least two flaws in fertilization cycles prior to immunotherapy. The immunization with paternal… Advisors/Committee Members: Barini, Ricardo, 1955- (advisor), Machado, Isabela Nelly (advisor), Júnior, Edward Araújo (committee member), Júnior, Renato Passini (committee member).

Subjects/Keywords: Infertilidade; Fertilização in vitro

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APA (6^th Edition):

Klimesch,Yvonne, 1. (2014). Avaliação da imunoterapia com linfócitos paternos em casais com falhas de implantação após fertilização in vitro = The influence of immunotherapy with paternal lymphocytes in the results of in vitro fertilization in couples with implantation failures . (Thesis). Universidade Estadual de Campinas. Retrieved from http://repositorio.unicamp.br/jspui/handle/REPOSIP/313018

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Klimesch,Yvonne, 1978-. “Avaliação da imunoterapia com linfócitos paternos em casais com falhas de implantação após fertilização in vitro = The influence of immunotherapy with paternal lymphocytes in the results of in vitro fertilization in couples with implantation failures .” 2014. Thesis, Universidade Estadual de Campinas. Accessed December 06, 2019. http://repositorio.unicamp.br/jspui/handle/REPOSIP/313018.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Klimesch,Yvonne, 1978-. “Avaliação da imunoterapia com linfócitos paternos em casais com falhas de implantação após fertilização in vitro = The influence of immunotherapy with paternal lymphocytes in the results of in vitro fertilization in couples with implantation failures .” 2014. Web. 06 Dec 2019.

Vancouver:

Klimesch,Yvonne 1. Avaliação da imunoterapia com linfócitos paternos em casais com falhas de implantação após fertilização in vitro = The influence of immunotherapy with paternal lymphocytes in the results of in vitro fertilization in couples with implantation failures . [Internet] [Thesis]. Universidade Estadual de Campinas; 2014. [cited 2019 Dec 06]. Available from: http://repositorio.unicamp.br/jspui/handle/REPOSIP/313018.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Klimesch,Yvonne 1. Avaliação da imunoterapia com linfócitos paternos em casais com falhas de implantação após fertilização in vitro = The influence of immunotherapy with paternal lymphocytes in the results of in vitro fertilization in couples with implantation failures . [Thesis]. Universidade Estadual de Campinas; 2014. Available from: http://repositorio.unicamp.br/jspui/handle/REPOSIP/313018

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

8. Renzi, Adriana. Análise da influência do hormônio anti-Mülleriano na produção in vitro de embriões bovinos.

Degree: PhD, Genética, 2012, University of São Paulo

URL: http://www.teses.usp.br/teses/disponiveis/17/17135/tde-19042013-154338/ ;

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A maturação in vitro de oócitos (MIV) é uma importante tecnologia reprodutiva a qual gera oócitos maduros capazes de suportar o desenvolvimento embrionário pré-implantacional e… (more)

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A maturação in vitro de oócitos (MIV) é uma importante tecnologia reprodutiva a qual gera oócitos maduros capazes de suportar o desenvolvimento embrionário pré-implantacional e sua completa evolução à termo. Muitos fatores levam ao processo de maturação do oócito, e o AMH (hormônio anti-Mülleriano) tem demonstrado possuir um importante efeito nesta etapa. Neste trabalho nós demonstramos a influência da suplementação de AMH na maturação de complexos cumulus-oócito (COCs). Nossos resultados demonstram que não houve efeito na produção de embriões para COCS grau I. Entretanto, pudemos encontrar diferenças significativas entre os COCs graus II e III maturados na presença de 150ng/ml de AMH. Aqui também demonstramos que não houve diferença significativa na expressão relativa de mRNA para os genes AMHRII e FSHR no oócito, e na expressão relativa de mRNA para os genes AMH, AMHRII e FSHR nas células da granulosa. Nossos resultados corroboram com as importantes funções do AMH na produção de embriões, sugerem que a suplementação do meio de maturação de oócitos com AMH pode ajudar a melhorar a produção de blastocistos.

The in vitro oocyte maturation (MIV) is an important reproductive technology that generates mature oocytes able to support the preimplantation embryonic development and their fully evolution to term. Many factors lead to oocyte maturation process, and the AMH (AntiMüllerian hormone) have demonstrated important effects in the oocyte development. Here, we report the influence of AMH supplementation in the cumulus-oocyte complex (COCs) maturation. We found that AMH had no effect on embryo production of COCs grade I. On the other hand, significant differences between the COCs grade II and COCs grade III matured in AMH 150ng/ml were verified. We have also demonstrated that there were no significant difference in mRNA expression of the genes AMHRII and FSHR in the oocyte, and in mRNA of the genes AMH, AMHRII and FSHR in the granulosa cells. Taken together, the results corroborate the important roles for AMH on embryo production, and suggest that AMH supplementation could achieve successful outcome in the production of blastocysts.

Advisors/Committee Members: Lôbo, Raysildo Barbosa.

Subjects/Keywords: Anti-Müllerian hormone.; Bovinos; Cattle; Fertilização In Vitro; Hormônio Anti-Mülleriano.; In Vitro Fertilization; In Vitro Maturation; Maturação In Vitro

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Renzi, A. (2012). Análise da influência do hormônio anti-Mülleriano na produção in vitro de embriões bovinos. (Doctoral Dissertation). University of São Paulo. Retrieved from http://www.teses.usp.br/teses/disponiveis/17/17135/tde-19042013-154338/ ;

Chicago Manual of Style (16^th Edition):

Renzi, Adriana. “Análise da influência do hormônio anti-Mülleriano na produção in vitro de embriões bovinos.” 2012. Doctoral Dissertation, University of São Paulo. Accessed December 06, 2019. http://www.teses.usp.br/teses/disponiveis/17/17135/tde-19042013-154338/ ;.

MLA Handbook (7^th Edition):

Renzi, Adriana. “Análise da influência do hormônio anti-Mülleriano na produção in vitro de embriões bovinos.” 2012. Web. 06 Dec 2019.

Vancouver:

Renzi A. Análise da influência do hormônio anti-Mülleriano na produção in vitro de embriões bovinos. [Internet] [Doctoral dissertation]. University of São Paulo; 2012. [cited 2019 Dec 06]. Available from: http://www.teses.usp.br/teses/disponiveis/17/17135/tde-19042013-154338/ ;.

Council of Science Editors:

Renzi A. Análise da influência do hormônio anti-Mülleriano na produção in vitro de embriões bovinos. [Doctoral Dissertation]. University of São Paulo; 2012. Available from: http://www.teses.usp.br/teses/disponiveis/17/17135/tde-19042013-154338/ ;

9. Mansseri née Lamrioui, Akila. Etude de la multiplicatoin par clonage "in vitro" du merisier (Prunus avium L.).

Degree: 2011, Université M'Hamed Bougara Boumerdès

URL: http://dlibrary.univ-boumerdes.dz:8080/jspui/handle/123456789/174

►

161 p. , ill. , 30 cm

Le présent travail a porté sur l’étude de la multiplication du Merisier (Prunus avium L.) par clonage in… (more)

Subjects/Keywords: Clonage; Multiplication in vitro; Merisier

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APA (6^th Edition):

Mansseri née Lamrioui, A. (2011). Etude de la multiplicatoin par clonage "in vitro" du merisier (Prunus avium L.). (Thesis). Université M'Hamed Bougara Boumerdès. Retrieved from http://dlibrary.univ-boumerdes.dz:8080/jspui/handle/123456789/174

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Mansseri née Lamrioui, Akila. “Etude de la multiplicatoin par clonage "in vitro" du merisier (Prunus avium L.).” 2011. Thesis, Université M'Hamed Bougara Boumerdès. Accessed December 06, 2019. http://dlibrary.univ-boumerdes.dz:8080/jspui/handle/123456789/174.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Mansseri née Lamrioui, Akila. “Etude de la multiplicatoin par clonage "in vitro" du merisier (Prunus avium L.).” 2011. Web. 06 Dec 2019.

Vancouver:

Mansseri née Lamrioui A. Etude de la multiplicatoin par clonage "in vitro" du merisier (Prunus avium L.). [Internet] [Thesis]. Université M'Hamed Bougara Boumerdès; 2011. [cited 2019 Dec 06]. Available from: http://dlibrary.univ-boumerdes.dz:8080/jspui/handle/123456789/174.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Mansseri née Lamrioui A. Etude de la multiplicatoin par clonage "in vitro" du merisier (Prunus avium L.). [Thesis]. Université M'Hamed Bougara Boumerdès; 2011. Available from: http://dlibrary.univ-boumerdes.dz:8080/jspui/handle/123456789/174

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Addis Ababa University

10. ASKALE, GIZAW. IN VITRO EFFECACY OF METHANOLIC EXTRACTS OF VERNONIA AMYGDALINA, CROTON MACROSTACHYUS, RICINUS COMMUNIS AND PETROLEUM ETHER EXTRACT OF MILLETTIA FERRUGINEA AGAINST BOVICOLA OVIS AND RHIPICEPHALUS (BOOPHILUS) DECOLORATUS .

Degree: 2015, Addis Ababa University

URL: http://etd.aau.edu.et/dspace/handle/123456789/7794

► In vitro loucicidal and accaricidal efficacy evaluation of Vernonia amygdalina, Croton macrostachyus, Ricinus communis and Millettia ferruginea against Bovicola ovis and Rhipicephalus decoloratus of cattle… (more)

▼ In vitro loucicidal and accaricidal efficacy evaluation of Vernonia amygdalina, Croton macrostachyus, Ricinus communis and Millettia ferruginea against Bovicola ovis and Rhipicephalus decoloratus of cattle were carried out from January 2014 to April 2015. Crude methanol extracts of Vernonia amygdalina, Croton macrostachyus, Ricinus communis leaf and Petroleum ether extract of Millettia ferruginea seed oil were prepared for in vitro test at different time intervals. The four selected medicinal plants, at concentrations of 200, 100, 50, 25, 12.5 and 6.25mg/ml and a commercially used acaricide (0.1% diazinon), were examined using in vitro adult immersion test. In vitro loucicidal test showed all concentration of Millettia ferruginea oil (µl/ml), ≥25mg /ml concentration of Croton macrostachyus and 200 and 100mg/ml concentration of Ricinus communis had pronounced loucidal activity at 24hr of exposure. 200mg/ml concentration of Vernonia amygdalina and ≤6.25mg/ml concentration of Croton macr ostachyus had moderate loucicidal activity at 24hr of exposure. Moreover, the 200 and 100µl/ml concentration of Millettia ferruginea seed oil and 200mg/ml concentration of Ricinus communis leaf crude extract had high accaricidal activity (90-100%) against Rhipicephalus decoloratus while crude methanolic extracts of Croton macrostachyus and Vernonia amygdalina and even the positive control (diazinon) showed low acaricidal activity (<35%) against the tick species. All plants had significantly (P< 0.05) higher activity against lice than Rhipicephalus decoloratus ticks except Millettia ferruginea at (200 and 100µl/ml) and Ricinus communis (at 200 mg/ml) which showed both accaricidal and loucicidal activity. Therefore the present study concluded that Croton macrostachyus, Ricinus communis and Millettia ferruginea against Bovicola ovis whereas Ricinus communis and Millettia ferruginea against Rhipicephalus decoloratus could be used as potential alternative in the discovery of guide compounds that substitute commercially available acaricides. In addition, the low activity of diazinon on ticks compared to our plant extracts deserves further attention. Advisors/Committee Members: Dr. Yacob Hailu (advisor).

Subjects/Keywords: Acaricide; Bovicola ovis; in vitro

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

ASKALE, G. (2015). IN VITRO EFFECACY OF METHANOLIC EXTRACTS OF VERNONIA AMYGDALINA, CROTON MACROSTACHYUS, RICINUS COMMUNIS AND PETROLEUM ETHER EXTRACT OF MILLETTIA FERRUGINEA AGAINST BOVICOLA OVIS AND RHIPICEPHALUS (BOOPHILUS) DECOLORATUS . (Thesis). Addis Ababa University. Retrieved from http://etd.aau.edu.et/dspace/handle/123456789/7794

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

ASKALE, GIZAW. “IN VITRO EFFECACY OF METHANOLIC EXTRACTS OF VERNONIA AMYGDALINA, CROTON MACROSTACHYUS, RICINUS COMMUNIS AND PETROLEUM ETHER EXTRACT OF MILLETTIA FERRUGINEA AGAINST BOVICOLA OVIS AND RHIPICEPHALUS (BOOPHILUS) DECOLORATUS .” 2015. Thesis, Addis Ababa University. Accessed December 06, 2019. http://etd.aau.edu.et/dspace/handle/123456789/7794.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

ASKALE, GIZAW. “IN VITRO EFFECACY OF METHANOLIC EXTRACTS OF VERNONIA AMYGDALINA, CROTON MACROSTACHYUS, RICINUS COMMUNIS AND PETROLEUM ETHER EXTRACT OF MILLETTIA FERRUGINEA AGAINST BOVICOLA OVIS AND RHIPICEPHALUS (BOOPHILUS) DECOLORATUS .” 2015. Web. 06 Dec 2019.

Vancouver:

ASKALE G. IN VITRO EFFECACY OF METHANOLIC EXTRACTS OF VERNONIA AMYGDALINA, CROTON MACROSTACHYUS, RICINUS COMMUNIS AND PETROLEUM ETHER EXTRACT OF MILLETTIA FERRUGINEA AGAINST BOVICOLA OVIS AND RHIPICEPHALUS (BOOPHILUS) DECOLORATUS . [Internet] [Thesis]. Addis Ababa University; 2015. [cited 2019 Dec 06]. Available from: http://etd.aau.edu.et/dspace/handle/123456789/7794.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

ASKALE G. IN VITRO EFFECACY OF METHANOLIC EXTRACTS OF VERNONIA AMYGDALINA, CROTON MACROSTACHYUS, RICINUS COMMUNIS AND PETROLEUM ETHER EXTRACT OF MILLETTIA FERRUGINEA AGAINST BOVICOLA OVIS AND RHIPICEPHALUS (BOOPHILUS) DECOLORATUS . [Thesis]. Addis Ababa University; 2015. Available from: http://etd.aau.edu.et/dspace/handle/123456789/7794

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Cornell University

11. Blakeley, Amy. Understanding The Effect Of Interface Chemistry In Biomineralization – Development Of An In Vitro Model For Calcium Phosphate Mineralization .

Degree: 2013, Cornell University

URL: http://hdl.handle.net/1813/33811

► Structure-function relationships in mineralized tissues, such as bone and teeth, depend upon the chemical interactions of the interfaces between cells, proteins, organic matrix, and mineral… (more)

▼ Structure-function relationships in mineralized tissues, such as bone and teeth, depend upon the chemical interactions of the interfaces between cells, proteins, organic matrix, and mineral (calcium phosphate). Development of in vitro models that more accurately model mineralization systems in vivo will assist in understanding medical conditions as well as inform biomimetic materials development. Understanding the heirachical structures in mineralized tissues relies on the chemical control of crystal nucleation and growth at the interfaces. Studies presented in this thesis provide additional in vitro tools for studying in vivo systems by controlling for interface chemistry, mineralization environment, and experimental setup for elucidating the effect of interfaces in biomineralization. Covalently bound surface chemistry - oxide, methyl, carboxylate, 2-aminoethyl dihydrogen phosphate, osteopontin, and bovine serum albumin (BSA) - on porous silicon substrates has a distinct affect on surface mineral nucleation and morphology. The morphology and coverage resulting from suspending the differed from those reported in literature. This method has potential for selectively examining the effects of covalently bound protein conformations and segments, more closely mimicking the conformation constraints at interfaces in biological systems. Hydrogel-based double diffusion system (DDS) has the potential to allow for the simultaneous in vitro study of cell-mineral-matrix interactions in a 3-D environment, the 'holy-grail' of mineralization studies. Successful hydroxyapatite mineralization was demonstrated at both room temperature and incubator conditions using a custom build polycarbonate DDS with polydimethylsiloxane membranes for gas permeability, minimal polyethyleneimine coating for gel adhesion, and cell culture compatible Agarose. This system provides researchers with a new tool for combining cell, mineral, and matrix in a 3-D environment for understanding the complexity of cell-mineral-matrix interactions. Understanding how proteins interact with surface chemistry as well as their structural changes and coverage, provides important clues towards biology's ability to exert control over biomineralization. Analysis of the monolayer-protein interface via infrared spectroscopy demonstrated that the protein conformation increases in disorder upon adsorption to the surface based on amide peak positions and amounts of adsorbed protein varied as a function of chemistry. Work in this thesis provides tools and information towards developing in vitro systems that elucidate complex interactions in mineralized tissues. Advisors/Committee Members: Van Dover, Robert B. (committeeMember), Estroff, Lara A. (committeeMember).

Subjects/Keywords: Biomineralization; interfaces; in vitro models

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APA (6^th Edition):

Blakeley, A. (2013). Understanding The Effect Of Interface Chemistry In Biomineralization – Development Of An In Vitro Model For Calcium Phosphate Mineralization . (Thesis). Cornell University. Retrieved from http://hdl.handle.net/1813/33811

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Blakeley, Amy. “Understanding The Effect Of Interface Chemistry In Biomineralization – Development Of An In Vitro Model For Calcium Phosphate Mineralization .” 2013. Thesis, Cornell University. Accessed December 06, 2019. http://hdl.handle.net/1813/33811.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Blakeley, Amy. “Understanding The Effect Of Interface Chemistry In Biomineralization – Development Of An In Vitro Model For Calcium Phosphate Mineralization .” 2013. Web. 06 Dec 2019.

Vancouver:

Blakeley A. Understanding The Effect Of Interface Chemistry In Biomineralization – Development Of An In Vitro Model For Calcium Phosphate Mineralization . [Internet] [Thesis]. Cornell University; 2013. [cited 2019 Dec 06]. Available from: http://hdl.handle.net/1813/33811.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Blakeley A. Understanding The Effect Of Interface Chemistry In Biomineralization – Development Of An In Vitro Model For Calcium Phosphate Mineralization . [Thesis]. Cornell University; 2013. Available from: http://hdl.handle.net/1813/33811

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Universiteit Utrecht

12. Marlen, R.L.M. van. Acclimatization, survival and growth of in vitro grown Arabidopsis thaliana seedlings after transplanting to soil.

Degree: 2015, Universiteit Utrecht

URL: http://dspace.library.uu.nl:8080/handle/1874/317684

► Plants grown in vitro have a different morphology, physiology and anatomy than plants grown in soil. In vitro grown plants usually have non-functional stomata, a… (more)

Subjects/Keywords: Arabidopsis thaliana; acclimatization; in vitro

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APA (6^th Edition):

Marlen, R. L. M. v. (2015). Acclimatization, survival and growth of in vitro grown Arabidopsis thaliana seedlings after transplanting to soil. (Masters Thesis). Universiteit Utrecht. Retrieved from http://dspace.library.uu.nl:8080/handle/1874/317684

Chicago Manual of Style (16^th Edition):

Marlen, R L M van. “Acclimatization, survival and growth of in vitro grown Arabidopsis thaliana seedlings after transplanting to soil.” 2015. Masters Thesis, Universiteit Utrecht. Accessed December 06, 2019. http://dspace.library.uu.nl:8080/handle/1874/317684.

MLA Handbook (7^th Edition):

Marlen, R L M van. “Acclimatization, survival and growth of in vitro grown Arabidopsis thaliana seedlings after transplanting to soil.” 2015. Web. 06 Dec 2019.

Vancouver:

Marlen RLMv. Acclimatization, survival and growth of in vitro grown Arabidopsis thaliana seedlings after transplanting to soil. [Internet] [Masters thesis]. Universiteit Utrecht; 2015. [cited 2019 Dec 06]. Available from: http://dspace.library.uu.nl:8080/handle/1874/317684.

Council of Science Editors:

Marlen RLMv. Acclimatization, survival and growth of in vitro grown Arabidopsis thaliana seedlings after transplanting to soil. [Masters Thesis]. Universiteit Utrecht; 2015. Available from: http://dspace.library.uu.nl:8080/handle/1874/317684

13. Kelle de Andrade Lemoine Neves, Juliana. Imidazolidinas esquistomicidas: avaliação ultraestrutural, atividade citotóxica e imunomoduladora .

Degree: 2010, Universidade Federal de Pernambuco

URL: http://repositorio.ufpe.br/handle/123456789/1273

► A esquistossomose é uma doença debilitante e endêmica, distribuída em 74 países, causada por parasitas do gênero Schistosoma, onde existem cinco espécies de interesse médico,… (more)

Subjects/Keywords: Esquistossomose; Imidazolidinadionas; Ensaios in vitro

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APA (6^th Edition):

Kelle de Andrade Lemoine Neves, J. (2010). Imidazolidinas esquistomicidas: avaliação ultraestrutural, atividade citotóxica e imunomoduladora . (Thesis). Universidade Federal de Pernambuco. Retrieved from http://repositorio.ufpe.br/handle/123456789/1273

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Kelle de Andrade Lemoine Neves, Juliana. “Imidazolidinas esquistomicidas: avaliação ultraestrutural, atividade citotóxica e imunomoduladora .” 2010. Thesis, Universidade Federal de Pernambuco. Accessed December 06, 2019. http://repositorio.ufpe.br/handle/123456789/1273.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Kelle de Andrade Lemoine Neves, Juliana. “Imidazolidinas esquistomicidas: avaliação ultraestrutural, atividade citotóxica e imunomoduladora .” 2010. Web. 06 Dec 2019.

Vancouver:

Kelle de Andrade Lemoine Neves J. Imidazolidinas esquistomicidas: avaliação ultraestrutural, atividade citotóxica e imunomoduladora . [Internet] [Thesis]. Universidade Federal de Pernambuco; 2010. [cited 2019 Dec 06]. Available from: http://repositorio.ufpe.br/handle/123456789/1273.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Kelle de Andrade Lemoine Neves J. Imidazolidinas esquistomicidas: avaliação ultraestrutural, atividade citotóxica e imunomoduladora . [Thesis]. Universidade Federal de Pernambuco; 2010. Available from: http://repositorio.ufpe.br/handle/123456789/1273

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Universidade Estadual de Campinas

14. Ratti, Alhethea, 1981-. Validação de um modelo de ciclagens de pH para avaliação do potencial anticárie de dentifrício de alta concentração de flúor em dentina radicular .

Degree: 2013, Universidade Estadual de Campinas

URL: http://repositorio.unicamp.br/jspui/handle/REPOSIP/290566

► Resumo: Considerando que dentifrícios de alta concentração de flúor (F) têm sido recomendados para reversão de lesões cariosas radiculares e que inexiste um modelo in… (more)

▼ Resumo: Considerando que dentifrícios de alta concentração de flúor (F) têm sido recomendados para reversão de lesões cariosas radiculares e que inexiste um modelo in vitro de ciclagens de pH que mostre efeito dose-resposta desses dentifrícios em dentina radicular, o objetivo do presente estudo foi desenvolver um modelo de ciclagens de pH para avaliação do potencial anticárie de dentifrício de alta concentração de F na inibição à desmineralização de dentina radicular. Foi realizado um estudo in vitro de ciclagens de pH por meio de dois experimentos. As unidades experimentais foram blocos de dentina bovina hígida, selecionados a partir dos valores de dureza de superfície. O regime de ciclagens de pH teve duração de 10 dias, e os blocos dentais foram mantidos diariamente por 6 h na solução desmineralizante e aproximadamente 18 h na solução remineralizante a 37?C. No primeiro experimento, para validação do modelo quanto ao efeito dose resposta ao F, soluções fluoretadas nas concentrações de 0, 50, 150, 450 e 1350 ?g F/mL foram utilizadas, simulando a diluição de dentifrícios fluoretados de baixa, alta e concentração convencional de F na saliva durante a escovação. Duas vezes por dia, antes e após a imersão na solução desmineralizante, os blocos dentais foram mantidos sob agitação por 5 min em temperatura ambiente nas soluções de tratamento descritas acima. O potencial anticárie do dentifrício de alta concentração de F foi avaliado em um segundo experimento, usando o modelo testado nas mesmas condições anteriormente descritas. Blocos dentais foram aleatoriamente divididos em 4 grupos de tratamento: dentifrício não fluoretado (DNF; controle negativo) e dentifrícios fluoretados comerciais contendo 500, 1100 e 5000 ?g de F/g. Todos os dentifrícios apresentavam a mesma formulação (NaF e sílica) e foram utilizados 2 vezes por dia na forma de suspensão na proporção 1:3 (dentifrício/água purificada). No primeiro experimento, as médias e desvios-padrão dos valores de área de lesão (?S; kg/mm2 x ?m; n=15) para os grupos tratados com água purificada e soluções contendo 50, 150, 450 e 1350 ?g F/mL foram respectivamente 1320,6± 298,6a; 1022,8 ± 317,7b; 748,9± 240,9b; 466,8 ± 246,3c e 163,1 ± 117,1d kg/mm2 x ?m. Quanto à concentração de F total na dentina (F total; ?g F/cm2; n=8), foram observados valores crescentes quanto maior a concentração de F nas soluções de tratamento (17,7 ± 3,6a; 27,3 ± 3,4ab; 33,1 ± 5,7bc; 41,3 ± 8,0cd e 52,6 ± 15,2d ?g F/cm2). No segundo experimento, as médias dos valores de ?S (n=12) para os grupos tratados com DNF, 500, 1100 e 5000 ?g F/g foram respectivamente 1328,1 ± 450,9a; 761,6 ± 308,8b; 705,6 ± 243,9b e 234,2 ± 198,0c kg/mm2 x ?m. Quanto à análise de F total na dentina no 2o experimento (n=8), as médias foram 10,2 ± 2,0a; 29,4 ± 6,4b; 34,3 ± 7,5b e 49,1 ± 7,3c ?g F/cm2. Conclui-se que o modelo in vitro de ciclagens de pH desenvolvido utilizando dentina radicular bovina apresentou efeito dose-resposta ao F e também foi capaz de avaliar o potencial anticárie de dentifrício de alta concentração de F;… Advisors/Committee Members: Tabchoury, Cinthia Pereira Machado, 1969- (advisor), Turssi, Cecilia Pedroso (committee member), Leme, Adriana Franco Paes (committee member).

Subjects/Keywords: In Vitro; Fluoretos; Cremes dentais

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Ratti, Alhethea, 1. (2013). Validação de um modelo de ciclagens de pH para avaliação do potencial anticárie de dentifrício de alta concentração de flúor em dentina radicular . (Thesis). Universidade Estadual de Campinas. Retrieved from http://repositorio.unicamp.br/jspui/handle/REPOSIP/290566

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Ratti, Alhethea, 1981-. “Validação de um modelo de ciclagens de pH para avaliação do potencial anticárie de dentifrício de alta concentração de flúor em dentina radicular .” 2013. Thesis, Universidade Estadual de Campinas. Accessed December 06, 2019. http://repositorio.unicamp.br/jspui/handle/REPOSIP/290566.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Ratti, Alhethea, 1981-. “Validação de um modelo de ciclagens de pH para avaliação do potencial anticárie de dentifrício de alta concentração de flúor em dentina radicular .” 2013. Web. 06 Dec 2019.

Vancouver:

Ratti, Alhethea 1. Validação de um modelo de ciclagens de pH para avaliação do potencial anticárie de dentifrício de alta concentração de flúor em dentina radicular . [Internet] [Thesis]. Universidade Estadual de Campinas; 2013. [cited 2019 Dec 06]. Available from: http://repositorio.unicamp.br/jspui/handle/REPOSIP/290566.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Ratti, Alhethea 1. Validação de um modelo de ciclagens de pH para avaliação do potencial anticárie de dentifrício de alta concentração de flúor em dentina radicular . [Thesis]. Universidade Estadual de Campinas; 2013. Available from: http://repositorio.unicamp.br/jspui/handle/REPOSIP/290566

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

15. Escrich Albelda, Laura. Maduración in vitro de ovocitos humanos desestimados de ciclos de estimulación ovárica.

Degree: 2018, TDX

URL: http://hdl.handle.net/10803/570644

► El éxito de las técnicas de reproducción asistida está directamente relacionado con el número y grado de madurez de los ovocitos recuperados tras la punción… (more)

▼ El éxito de las técnicas de reproducción asistida está directamente relacionado con el número y grado de madurez de los ovocitos recuperados tras la punción folicular, siendo únicamente útiles reproductivamente, aquéllos maduros en estadio de MII; existiendo un 11% en estadio inmaduro (VG) y, por tanto, no útiles reproductivamente. Por ello, planteamos el rescate de ovocitos inmaduros en estadio VG como estrategia reproductiva a fin de aumentar el número de ovocitos iniciales. El objetivo de esta tesis es optimizar las tasas de maduración in vitro (MIV) a nivel nuclear, citoplasmático y genómico de los ovocitos humanos en estadio de VG, recuperados en ciclos de estimulación ovárica controlada con indicación de ICSI. Para ello se han realizado 3 estudios. En el primero, se evaluó la correlación de ciertos parámetros morfométricos y morfológicos no invasivos con la condensación y distribución de la cromatina, en relación al nucléolo. Tras analizar 131 VG, éstas se agruparon retrospectivamente en 3 modelos y las variables morfométricas y morfológicas comparadas. Tras agrupar las VG en función de la condensación de la cromatina, se observó que las variables: diámetro ovocitario, continuidad de la envoltura nuclear, posición del nucléolo y apariencia del nucleoplasma permitieron predecir, mediante un modelo matemático, el grado de condensación de la cromatina, manteniendo la viabilidad del ovocito. En el segundo estudio, se evaluó la maduración ovocitaria in vitro y su correlación con el grado de condensación de la cromatina. Para ello, 131 VG fueron cultivadas en un sistema convencional (SC) y, el grado de maduración nuclear alcanzado evaluado a intervalos regulares de tiempo. Tras 24h, la tasa de maduración nuclear, o capacidad de los ovocitos VG para progresar in vitro a MII, fue del 55%, aumentando al 75% tras prolongar el cultivo hasta 42h. Respecto a la competencia citoplasmática, o capacidad de los ovocitos MII de responder a un procedimiento de activación ovocitaria artificial, un 64% de los ovocitos MIV activó, porcentaje significativamente inferior al observado en ovocitos control (100%). Igualmente, la tasa de activación normal, fue significativamente inferior en el grupo de MIV (22.4% vs. 79.2%). Respeto a la competencia genómica o determinación de la ploidía por FISH observamos que todos los ovocitos con respuesta normal fueron haploides. Finalmente, tras aplicar el modelo matemático predictivo del grado de condensación de la cromatina, observamos que esta variable no muestra relación alguna con la competencia nuclear, citoplasmática o genómica de los ovocitos VG, posiblemente debido a la homogeneidad de la población, en términos de condensación de la cromatina. En el tercero estudio, se describió la cinética de la maduración nuclear in vitro y su correlación con la competencia citoplasmática. Las VG cultivadas en sistema time-lapse (STL) mostraron una tasa de maduración nuclear superior a la obtenida tras cultivo en SC (88.9% vs. 74.8%); además, su competencia citoplasmática fue también superior (STL:… Advisors/Committee Members: Escribá Pérez, Mª José, Departament de Bioquímica i Biologia Molecular.

Subjects/Keywords: Maduración in vitro; Ovocito

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Escrich Albelda, L. (2018). Maduración in vitro de ovocitos humanos desestimados de ciclos de estimulación ovárica. (Thesis). TDX. Retrieved from http://hdl.handle.net/10803/570644

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Escrich Albelda, Laura. “Maduración in vitro de ovocitos humanos desestimados de ciclos de estimulación ovárica.” 2018. Thesis, TDX. Accessed December 06, 2019. http://hdl.handle.net/10803/570644.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Escrich Albelda, Laura. “Maduración in vitro de ovocitos humanos desestimados de ciclos de estimulación ovárica.” 2018. Web. 06 Dec 2019.

Vancouver:

Escrich Albelda L. Maduración in vitro de ovocitos humanos desestimados de ciclos de estimulación ovárica. [Internet] [Thesis]. TDX; 2018. [cited 2019 Dec 06]. Available from: http://hdl.handle.net/10803/570644.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Escrich Albelda L. Maduración in vitro de ovocitos humanos desestimados de ciclos de estimulación ovárica. [Thesis]. TDX; 2018. Available from: http://hdl.handle.net/10803/570644

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

16. Afonso, Ricardo. Estudos sobre aproveitamento biotecnológico da quitina produzida por organismos marinhos.

Degree: 2008, RCAAP

URL: http://www.rcaap.pt/detail.jsp?id=oai:sapientia.ualg.pt:10400.1/710

►

Dissertação mest., Biotecnologia, Universidade do Algarve, 2008

O oceano é um dos mais ricos habitats do planeta, mas também um dos menos explorados, embora a… (more)

Subjects/Keywords: Teses; Biotecnologia; Cultura in vitro

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Afonso, R. (2008). Estudos sobre aproveitamento biotecnológico da quitina produzida por organismos marinhos. (Thesis). RCAAP. Retrieved from http://www.rcaap.pt/detail.jsp?id=oai:sapientia.ualg.pt:10400.1/710

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Afonso, Ricardo. “Estudos sobre aproveitamento biotecnológico da quitina produzida por organismos marinhos.” 2008. Thesis, RCAAP. Accessed December 06, 2019. http://www.rcaap.pt/detail.jsp?id=oai:sapientia.ualg.pt:10400.1/710.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Afonso, Ricardo. “Estudos sobre aproveitamento biotecnológico da quitina produzida por organismos marinhos.” 2008. Web. 06 Dec 2019.

Vancouver:

Afonso R. Estudos sobre aproveitamento biotecnológico da quitina produzida por organismos marinhos. [Internet] [Thesis]. RCAAP; 2008. [cited 2019 Dec 06]. Available from: http://www.rcaap.pt/detail.jsp?id=oai:sapientia.ualg.pt:10400.1/710.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Afonso R. Estudos sobre aproveitamento biotecnológico da quitina produzida por organismos marinhos. [Thesis]. RCAAP; 2008. Available from: http://www.rcaap.pt/detail.jsp?id=oai:sapientia.ualg.pt:10400.1/710

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Debrecen

17. Kovács, Álmos. Vörös elemi nanoszelén és nátrium-szelenát biológiai hatásának összehasonlító vizsgálata in vitro dohánytenyészetben .

Degree: DE – ATC – Mezőgazdaság- Élelmiszertudományi és Környezetgazdálkodási Kar, 2011, University of Debrecen

URL: http://hdl.handle.net/2437/117878

► A szelén a legintenzívebben kutatott mikroelemek közé tartozik napjainkban. A nagy érdeklődés annak köszönhető, hogy bebizonyosodott esszenciális volta magassabbrendű állatokban, és az emberben is. Magasabbrendű… (more)

▼ A szelén a legintenzívebben kutatott mikroelemek közé tartozik napjainkban. A nagy érdeklődés annak köszönhető, hogy bebizonyosodott esszenciális volta magassabbrendű állatokban, és az emberben is. Magasabbrendű növényekben eddig nem bizonyították létfontosságú szerepét, bár pozitív hatását sok esetben tapasztalták alacsony koncentrációtartományban módosulatoktól függően. Növényekkel végzett kísérletekben a leggyakrabban használt szelénformák a szelenát illetve szelenit. E két forma fordul elő leggyakrabban a természetben és a növények számára a leginkább hozzáférhető módosulatok, ezzel magyarázható elterjedt alkalmazásuk. Van azonban egy érdekes, állatkísérletekben ígéretesnek tűnő szelénforma, a vörös elemi nanoszelén, melyet növényekben eddig még sohasem vizsgáltak. Dr. Prokisch József révén rendelkezésünkre állt tisztított nanoszelén szuszpenzió, így került kísérleteink homlokterébe. A kísérleteket dohány kallusztenyészetben és a mikrohajtásokból regenerálódó növényekben végeztem. Összehasonlításként a leggyakrabban használt szervetlen szelénformát, nátrium-szelenátot alkalmaztam. Eredményeink alapján megállapítható, hogy a vörös elemi nanoszelén partikulumok képesek bejutni a dohány in vitro szövettenyészetében, s ott a kalluszon át képződő mikrohajtások képződését stimulálta (különösen 50 - 100 mg/l koncentráció tartományban). A képződő mikrohajtások szabályos formájúak voltak normál szöveti szerkezettel, vitrifikáció megjelenése nélkül. Ez azért volt különösen szembetűnő, mivel a kontrol táptalajon a képződött mikrohajtások jelentős része abnormális alakú volt, áttetsző, vizenyős szöveti szerkezetük vitrifikációra utalt. Nanose-nel ellentétben szelenát esetében 10 mg/l koncentrációig indult meg a kalluszosodás, 50-100 mg/l tartomány azonban már gátolta a kalluszképződést. A nanoszelén hatását vizsgálva regenerálódó növényekben azt tapasztaltuk, hogy az főként a gyökérrészben akkumulálódott, ahol extrém magas értékeket tudtunk kimutatni. A hajtásrészben kis mértékben szállítódott fel, amit a transzport index számítása is alátámasztott. A nanoSe-nel szemben a szelenát forma főként hajtásrészben halmozódott fel, s a gyökérben csak kis mennyiség maradt. Az átoltott, regenerálódó hajtástenyészetekre is jellemző volt az a tendencia, amely a kalluszképződésnél is látható volt: e szerint ≥50 mg/l szelenát teljesen megakadályozta a gyökérképződést, a hajtások egy ideig vegetáltak, majd elpusztultak. Ezzel szemben a magas nanoSe tartalom (50-100 mg/l) kifejezetten segítette a gyökérképződés megindulását és a gyökerek frisstömeg gyarapodását is. Feltételezésünk szerint a szelenát és a nanoSe közötti biológiai hatásban tapasztalt különbség a két szelénforma kémiai természetéből adódhat. A szelenát, mint ionos forma bejutva a szövetekbe hamarabb okoz toxikus tüneteket. Ezt támasztotta alá a morfológiai vizsgálataink és a klorofilltartalom méréseink is. Az elemi nanoszelén azonban önmagában indifferens a növény számára. Vizes közegben azonban nagyon lassan ionok válhatnak le… Advisors/Committee Members: Fári, Miklós (advisor).

Subjects/Keywords: nanoszelén; in vitro dohány tenyészet

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Kovács, �. (2011). Vörös elemi nanoszelén és nátrium-szelenát biológiai hatásának összehasonlító vizsgálata in vitro dohánytenyészetben . (Thesis). University of Debrecen. Retrieved from http://hdl.handle.net/2437/117878

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Kovács, Álmos. “Vörös elemi nanoszelén és nátrium-szelenát biológiai hatásának összehasonlító vizsgálata in vitro dohánytenyészetben .” 2011. Thesis, University of Debrecen. Accessed December 06, 2019. http://hdl.handle.net/2437/117878.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Kovács, Álmos. “Vörös elemi nanoszelén és nátrium-szelenát biológiai hatásának összehasonlító vizsgálata in vitro dohánytenyészetben .” 2011. Web. 06 Dec 2019.

Vancouver:

Kovács �. Vörös elemi nanoszelén és nátrium-szelenát biológiai hatásának összehasonlító vizsgálata in vitro dohánytenyészetben . [Internet] [Thesis]. University of Debrecen; 2011. [cited 2019 Dec 06]. Available from: http://hdl.handle.net/2437/117878.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Kovács �. Vörös elemi nanoszelén és nátrium-szelenát biológiai hatásának összehasonlító vizsgálata in vitro dohánytenyészetben . [Thesis]. University of Debrecen; 2011. Available from: http://hdl.handle.net/2437/117878

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

18. Calheiros, Ana Manuel Casanova de Girão. Relação ácido-base na absorção de fármacos.

Degree: 2013, RCAAP

URL: https://www.rcaap.pt/detail.jsp?id=oai:comum.rcaap.pt:10400.26/14121

►

Dissertação para obtenção do grau de Mestre no Instituto Superior de Ciências da Saúde Egas Moniz

Neste trabalho são apresentados conceitos sobre a absorção de… (more)

▼

Dissertação para obtenção do grau de Mestre no Instituto Superior de Ciências da Saúde Egas Moniz

Neste trabalho são apresentados conceitos sobre a absorção de fármacos e em que aspecto a relação ácido-base a pode influenciar. A absorção consiste na transferência do fármaco do local de administração até à corrente sanguínea. Os fármacos podem ser administrados por diversas vias, sendo a via oral a mais destacada nesta dissertação, devido à grande variação de pH ao longo do TGI (de 1.3 a 7.5), o que implica diversas reacções ácido-base que podem afectar a absorção de fármacos. Ao longo do TGI, o grau de ionização é fundamental para a passagem através das membranas celulares, pois seja qual for a natureza dos fármacos, geralmente, a taxa de absorção por difusão passiva aumenta com um baixo grau de ionização, ou seja com o fármaco na forma não-ionizada. No entanto, para além do pH fisiológico, existem diversos factores, como a formulação do próprio fármaco, as interacções com outros medicamentos e com alimentos, que podem influenciar a dissolução, solubilidade e grau de ionização do fármaco. A indústria farmacêutica tem vindo a desenvolver estratégias e modelos in vitro, de modo a melhorar a formulação e certificar as propriedades dos fármacos que intervêm na sua absorção. Ao introduzir lauril sulfato de sódio (LSS), um surfactante, na fase externa da formulação do fármaco, aumenta a solubilidade e.g. do cetoprofeno, quer na forma ionizada ou não-ionizada. A adição de polímeros como o Kollicoat®SR 30D e o Kollicoat®MAE 30DP para aumentar a solubilidade e permeabilidade de fármacos quando a sua absorção fica comprometida com o valor de pH do meio e.g. do verapamilo. A associação dos modelos in vitro Caco-2, em que é usado a linhagem celular de adenocarcinoma do cólon humano, e o PAMPA (permeação em membrana artificial paralela) são ideais para a previsão da permeabilidade intestinal, permitindo priorizar as moléculas com propriedades mais favoráveis.

Advisors/Committee Members: Ferreira, Ana Paula.

Subjects/Keywords: Absorção; Ionização; TGI; In vitro

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Calheiros, A. M. C. d. G. (2013). Relação ácido-base na absorção de fármacos. (Thesis). RCAAP. Retrieved from https://www.rcaap.pt/detail.jsp?id=oai:comum.rcaap.pt:10400.26/14121

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Calheiros, Ana Manuel Casanova de Girão. “Relação ácido-base na absorção de fármacos.” 2013. Thesis, RCAAP. Accessed December 06, 2019. https://www.rcaap.pt/detail.jsp?id=oai:comum.rcaap.pt:10400.26/14121.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Calheiros, Ana Manuel Casanova de Girão. “Relação ácido-base na absorção de fármacos.” 2013. Web. 06 Dec 2019.

Vancouver:

Calheiros AMCdG. Relação ácido-base na absorção de fármacos. [Internet] [Thesis]. RCAAP; 2013. [cited 2019 Dec 06]. Available from: https://www.rcaap.pt/detail.jsp?id=oai:comum.rcaap.pt:10400.26/14121.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Calheiros AMCdG. Relação ácido-base na absorção de fármacos. [Thesis]. RCAAP; 2013. Available from: https://www.rcaap.pt/detail.jsp?id=oai:comum.rcaap.pt:10400.26/14121

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

19. Castro Modesto, Tania Milagros. Efecto del fluido folicular de alpaca en la maduración ovocitaria in vitro de alpaca (Vicugna pacos).

Degree: 2018, National University of San Marcos

URL: http://cybertesis.unmsm.edu.pe/handle/cybertesis/9692

► Evalúa el efecto del fluido folicular como suplemento en el medio de maduración sobre las tasas de maduración in vitro de ovocitos de alpaca. Las… (more)

▼ Evalúa el efecto del fluido folicular como suplemento en el medio de maduración sobre las tasas de maduración in vitro de ovocitos de alpaca. Las muestras biológicas fueron colectadas en el camal municipal de Huancavelica y trasladadas en una solución de NaCl al 0.9% a 4 ºC. En el laboratorio, los complejos cumulus-oophorus (CCO) fueron aislados de los folículos mediante dos métodos (aspiración y cortes) y los CCOs seleccionados fueron colocados en un medio de maduración suplementado con suero fetal bovino (SFB), fluido folicular (FF) o ambos (SFB+FF) y cultivados a 38.5 ºC, 5% de CO2 y 100% de humedad relativa durante 32 a 36 horas. La madurez de los ovocitos fue evaluada post-cultivo mediante la presencia del primer cuerpo polar. Para determinar la capacidad de los ovocitos maduros para completar los procesos de fertilización y desarrollo embrionario se realizaron 4 ensayos de FIV. Se trabajó con espermatozoides aislados de la zona caudal del epidídimo los que fueron seleccionados por el método de swim-up. La fertilización se realizó con una concentración de 2x106 esp/mL en medio HAM suplementado con heparina y PHE como agentes capacitantes. Los espermatozoides y ovocitos se cultivaron juntos bajo condiciones similares a las usadas para la maduración durante 18 horas, luego del cual, los ovocitos fueran lavados y cultivados en medio KSOM suplementado con SFB, piruvato sódico y gentamicina durante 7 días. En primer lugar, se comparó el promedio de CCO aislados mediante los métodos utilizados. Se obtuvo un promedio de 2.1 CCO aislados por ovario mediante el método de aspiración, mientras que con el método de cortes se obtuvo un promedio de 1.2 CCO por ovario, encontrándose diferencia significativa entre estos resultados (p˃0.05). En conclusión, los resultados sugieren que el método de aspiración muestra una mayor proporción de aislamiento de CCO en comparación con el método de cortes, asimismo se demuestra que el FF puede ser usado como un suplemento natural equivalente al SFB en los medios de maduración in vitro para ovocitos de alpaca.

Subjects/Keywords: Alpacas - Reproducción; Fecundación in vitro

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APA (6^th Edition):

Castro Modesto, T. M. (2018). Efecto del fluido folicular de alpaca en la maduración ovocitaria in vitro de alpaca (Vicugna pacos). (Thesis). National University of San Marcos. Retrieved from http://cybertesis.unmsm.edu.pe/handle/cybertesis/9692

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Castro Modesto, Tania Milagros. “Efecto del fluido folicular de alpaca en la maduración ovocitaria in vitro de alpaca (Vicugna pacos).” 2018. Thesis, National University of San Marcos. Accessed December 06, 2019. http://cybertesis.unmsm.edu.pe/handle/cybertesis/9692.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Castro Modesto, Tania Milagros. “Efecto del fluido folicular de alpaca en la maduración ovocitaria in vitro de alpaca (Vicugna pacos).” 2018. Web. 06 Dec 2019.

Vancouver:

Castro Modesto TM. Efecto del fluido folicular de alpaca en la maduración ovocitaria in vitro de alpaca (Vicugna pacos). [Internet] [Thesis]. National University of San Marcos; 2018. [cited 2019 Dec 06]. Available from: http://cybertesis.unmsm.edu.pe/handle/cybertesis/9692.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Castro Modesto TM. Efecto del fluido folicular de alpaca en la maduración ovocitaria in vitro de alpaca (Vicugna pacos). [Thesis]. National University of San Marcos; 2018. Available from: http://cybertesis.unmsm.edu.pe/handle/cybertesis/9692

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Universitat de Valencia

20. Fernández-Blanco Gómez, Celia. Estudios In Vitro de los mecanismos de toxicidad de las micotoxinas .

Degree: 2017, Universitat de Valencia

URL: http://hdl.handle.net/10550/58372

► Se ha llevado a cabo la evaluación in vitro de los efectos producidos por micotoxinas de Alternaria y Fusarium en células de mamífero. Se ha… (more)

▼ Se ha llevado a cabo la evaluación in vitro de los efectos producidos por micotoxinas de Alternaria y Fusarium en células de mamífero. Se ha evaluado la citotoxicidad individual del alternariol (AOH), alternariol monometil éter (AME), beauvericina (BEA), deoxinivalenol (DON), eniatina B (ENN B), fumonisina B1 (FB1), zearalenona (ZEA) y α-zearalenol (α-ZOL) en células de adenocarcinoma de colon humano (Caco-2) donde únicamente se obtuvieron valores de IC50 para la ENN B, DON, BEA y α- ZOL. La evaluación de la citotoxicidad combinada entre mezclas de micotoxinas en células Caco-2 mostró efecto sinérgico en las combinaciones de AOH+AME, DON+AOH y ENN B+AOH; efecto aditivo en la combinación DON+ENN B y efecto antagonista en la combinación terciaria DON+AOH+ENN B. Se estudió la bioaccesibilidad y biodisponibilidad del AOH, ZEA y α-ZOL. Se evaluó la bioaccesibilidad mediante el método de digestión estático in vitro, siendo el α-ZOL más bioaccesible a nivel gástrico y duodenal que la ZEA. Se observó una baja biodisponibilidad en las tres micotoxinas ensayadas con las células Caco-2/TC7, siendo el AOH el más biodisponible. Teniendo en cuenta la biodisponibilidad de las micotoxinas y los escasos estudios de mecanismos de toxicidad conocidos, se estudió la interacción de las micotoxinas con los componentes y la alteración de actividades celulares. Los resultados obtenidos demostraron que el AOH bloquea el ciclo celular en la fase G2/M, causa pérdida del potencial de la membrana mitocondrial y produce apoptosis a través de la vía mitocondrial. Además, se evaluó el daño causado por el AOH a nivel del ADN mediante el ensayo del cometa y se observó un incremento del daño dependiente de la concentración. Debido a la biodisponibilidad de las micotoxinas, se determinó la actividad estrogénica de algunas muy prevalentes como la FB1 y la BEA, observándose que la BEA produce mayor actividad estrogénica sobre células que la FB1. Dado que un mecanismo de citotoxicidad es el estrés oxidativo, se determina la capacidad del AOH para generar especies reactivas de oxígeno (ROS), evidenciándose que el AOH en las células Caco-2 produce ROS inmediatamente tras la exposición en todas lasconcentraciones ensayadas. Una de las consecuencias de las ROS es la oxidación de los lípidos de las membranas celulares, es decir, la generación de peroxidación lipídica (LPO). Los resultados obtenidos demostraron que el AOH aumenta significativamente la producción de LPO. Tras los resultados obtenidos se procedió a determinar la eficacia del sistema de defensa intracelular (enzimático y no enzimático) frente al estrés oxidativo. Estos indicaron un incremento de la actividad de la superóxido dismutasa (SOD) a todas las concentraciones de AOH expuestas en las células Caco-2 y que la actividad enzimática de la catalasa (CAT) fue más eficaz que la glutatión peroxidasa (GPx), eliminando peróxido de hidrógeno. Además, se demostró que el glutatión (GSH) y las enzimas implicadas en el … Advisors/Committee Members: Ruiz Leal, María José (advisor).

Subjects/Keywords: Micotoxinas; in vitro; Toxicología

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APA (6^th Edition):

Fernández-Blanco Gómez, C. (2017). Estudios In Vitro de los mecanismos de toxicidad de las micotoxinas . (Doctoral Dissertation). Universitat de Valencia. Retrieved from http://hdl.handle.net/10550/58372

Chicago Manual of Style (16^th Edition):

Fernández-Blanco Gómez, Celia. “Estudios In Vitro de los mecanismos de toxicidad de las micotoxinas .” 2017. Doctoral Dissertation, Universitat de Valencia. Accessed December 06, 2019. http://hdl.handle.net/10550/58372.

MLA Handbook (7^th Edition):

Fernández-Blanco Gómez, Celia. “Estudios In Vitro de los mecanismos de toxicidad de las micotoxinas .” 2017. Web. 06 Dec 2019.

Vancouver:

Fernández-Blanco Gómez C. Estudios In Vitro de los mecanismos de toxicidad de las micotoxinas . [Internet] [Doctoral dissertation]. Universitat de Valencia; 2017. [cited 2019 Dec 06]. Available from: http://hdl.handle.net/10550/58372.

Council of Science Editors:

Fernández-Blanco Gómez C. Estudios In Vitro de los mecanismos de toxicidad de las micotoxinas . [Doctoral Dissertation]. Universitat de Valencia; 2017. Available from: http://hdl.handle.net/10550/58372

Universitat de Valencia

21. Escrich Albelda, Laura. Maduración in vitro de ovocitos humanos desestimados de ciclos de estimulación ovárica .

Degree: 2015, Universitat de Valencia

URL: http://hdl.handle.net/10550/50735

► El éxito de las técnicas de reproducción asistida está directamente relacionado con el número y grado de madurez de los ovocitos recuperados tras la punción… (more)

▼ El éxito de las técnicas de reproducción asistida está directamente relacionado con el número y grado de madurez de los ovocitos recuperados tras la punción folicular, siendo únicamente útiles reproductivamente, aquéllos maduros en estadio de MII; existiendo un 11% en estadio inmaduro (VG) y, por tanto, no útiles reproductivamente. Por ello, planteamos el rescate de ovocitos inmaduros en estadio VG como estrategia reproductiva a fin de aumentar el número de ovocitos iniciales. El objetivo de esta tesis es optimizar las tasas de maduración in vitro (MIV) a nivel nuclear, citoplasmático y genómico de los ovocitos humanos en estadio de VG, recuperados en ciclos de estimulación ovárica controlada con indicación de ICSI. Para ello se han realizado 3 estudios. En el primero, se evaluó la correlación de ciertos parámetros morfométricos y morfológicos no invasivos con la condensación y distribución de la cromatina, en relación al nucléolo. Tras analizar 131 VG, éstas se agruparon retrospectivamente en 3 modelos y las variables morfométricas y morfológicas comparadas. Tras agrupar las VG en función de la condensación de la cromatina, se observó que las variables: diámetro ovocitario, continuidad de la envoltura nuclear, posición del nucléolo y apariencia del nucleoplasma permitieron predecir, mediante un modelo matemático, el grado de condensación de la cromatina, manteniendo la viabilidad del ovocito. En el segundo estudio, se evaluó la maduración ovocitaria in vitro y su correlación con el grado de condensación de la cromatina. Para ello, 131 VG fueron cultivadas en un sistema convencional (SC) y, el grado de maduración nuclear alcanzado evaluado a intervalos regulares de tiempo. Tras 24h, la tasa de maduración nuclear, o capacidad de los ovocitos VG para progresar in vitro a MII, fue del 55%, aumentando al 75% tras prolongar el cultivo hasta 42h. Respecto a la competencia citoplasmática, o capacidad de los ovocitos MII de responder a un procedimiento de activación ovocitaria artificial, un 64% de los ovocitos MIV activó, porcentaje significativamente inferior al observado en ovocitos control (100%). Igualmente, la tasa de activación normal, fue significativamente inferior en el grupo de MIV (22.4% vs. 79.2%). Respeto a la competencia genómica o determinación de la ploidía por FISH observamos que todos los ovocitos con respuesta normal fueron haploides. Finalmente, tras aplicar el modelo matemático predictivo del grado de condensación de la cromatina, observamos que esta variable no muestra relación alguna con la competencia nuclear, citoplasmática o genómica de los ovocitos VG, posiblemente debido a la homogeneidad de la población, en términos de condensación de la cromatina. En el tercero estudio, se describió la cinética de la maduración nuclear in vitro y su correlación con la competencia citoplasmática. Las VG cultivadas en sistema time-lapse (STL) mostraron una tasa de maduración nuclear superior a la obtenida tras cultivo en SC (88.9% vs. 74.8%); además, su competencia citoplasmática fue también superior (STL:… Advisors/Committee Members: Escribá Pérez, Mª José (advisor).

Subjects/Keywords: Maduración in vitro; Ovocito

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Escrich Albelda, L. (2015). Maduración in vitro de ovocitos humanos desestimados de ciclos de estimulación ovárica . (Doctoral Dissertation). Universitat de Valencia. Retrieved from http://hdl.handle.net/10550/50735

Chicago Manual of Style (16^th Edition):

Escrich Albelda, Laura. “Maduración in vitro de ovocitos humanos desestimados de ciclos de estimulación ovárica .” 2015. Doctoral Dissertation, Universitat de Valencia. Accessed December 06, 2019. http://hdl.handle.net/10550/50735.

MLA Handbook (7^th Edition):

Escrich Albelda, Laura. “Maduración in vitro de ovocitos humanos desestimados de ciclos de estimulación ovárica .” 2015. Web. 06 Dec 2019.

Vancouver:

Escrich Albelda L. Maduración in vitro de ovocitos humanos desestimados de ciclos de estimulación ovárica . [Internet] [Doctoral dissertation]. Universitat de Valencia; 2015. [cited 2019 Dec 06]. Available from: http://hdl.handle.net/10550/50735.

Council of Science Editors:

Escrich Albelda L. Maduración in vitro de ovocitos humanos desestimados de ciclos de estimulación ovárica . [Doctoral Dissertation]. Universitat de Valencia; 2015. Available from: http://hdl.handle.net/10550/50735

Virginia Tech

22. Vu, Lucas Trung. Proteomic Analysis of Three Dimensional Organotypic Liver Models.

Degree: PhD, Chemical Engineering, 2015, Virginia Tech

URL: http://hdl.handle.net/10919/77033

► In vitro liver models that closely mimic the in vivo microenvironment are central for understanding hepatic functions and intercellular communication processes. Bottom-up shotgun proteomic analysis… (more)

▼ In vitro liver models that closely mimic the in vivo microenvironment are central for understanding hepatic functions and intercellular communication processes. Bottom-up shotgun proteomic analysis of the hepatic cells can lend insight into such processes. This technique employs liquid chromatography-tandem mass spectrometry (LC-MS/MS) for relative quantification of protein abundances by measuring intensities of their corresponding peptides. Organotypic 3D liver models have been developed in our laboratory that consist of hepatocytes and liver sinusoidal endothelial cells (LSECs) separated by a polyelectrolyte multilayer (PEM), which serves as a mimic for the Space of Disse. Each component within these models is easily separable allowing for systematic evaluation of the cells and PEMs. In this study, proteomes of hepatocytes from PEM containing models, cultured with and without LSECs, were compared to those from monolayers. Changes in core metabolism were evaluated among all culture conditions. Overall, all cultures were ketogenic and performed gluconeogenesis. The presence of the PEM led to increases in proteins associated with mitochondrial-based β-oxidation and peroxisomal proteins. The PEMs also limited production of structural proteins, which are linked to dedifferentiation of hepatocytes, suggesting that cell-ECM interactions are essential for maintenance of their liver-like state. The presence of LSECs increased levels of carboxylesterases and other phase I and phase II detoxification enzymes suggesting that intercellular signaling mediates enzyme abundance. Taken together, these results suggest that the cell-cell (from the LSECs) and cell-ECM (from the PEMs) interactions exert different, yet crucial effects, and both are required for the preservation of metabolic liver functions and differentiated phenotypes. Changes in the PEMs as a result of cell culture were also evaluated but exhibited minimal differences at this time point. Proteomes of LSECs monolayers were also characterized. Enzymes related to the metabolism of amino acids, lipids, oxidative phosphorylation and phase I and phase II detoxification processes were all identified in LSECs monolayers highlighting their role in these processes. Characterization of 3DHL LSECs was not possible due to ion suppression resulting from the presence of excess contaminant proteins. Nonetheless, this study provides a foundation in which LSECs from 3D liver models can be compared against in future studies. Advisors/Committee Members: Rajagopalan, Padmavathy (committeechair), Helm, Richard F. (committeechair), Murali, T M. (committee member), Lu, Chang (committee member), Davis, Richey M. (committee member).

Subjects/Keywords: Shotgun Proteomics; Liver; In vitro

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APA (6^th Edition):

Vu, L. T. (2015). Proteomic Analysis of Three Dimensional Organotypic Liver Models. (Doctoral Dissertation). Virginia Tech. Retrieved from http://hdl.handle.net/10919/77033

Chicago Manual of Style (16^th Edition):

Vu, Lucas Trung. “Proteomic Analysis of Three Dimensional Organotypic Liver Models.” 2015. Doctoral Dissertation, Virginia Tech. Accessed December 06, 2019. http://hdl.handle.net/10919/77033.

MLA Handbook (7^th Edition):

Vu, Lucas Trung. “Proteomic Analysis of Three Dimensional Organotypic Liver Models.” 2015. Web. 06 Dec 2019.

Vancouver:

Vu LT. Proteomic Analysis of Three Dimensional Organotypic Liver Models. [Internet] [Doctoral dissertation]. Virginia Tech; 2015. [cited 2019 Dec 06]. Available from: http://hdl.handle.net/10919/77033.

Council of Science Editors:

Vu LT. Proteomic Analysis of Three Dimensional Organotypic Liver Models. [Doctoral Dissertation]. Virginia Tech; 2015. Available from: http://hdl.handle.net/10919/77033

Cornell University

23. McAuliffe, Gretchen. Development of Physiologically Realistic In Vitro Models of the Gastrointestinal Tract .

Degree: 2008, Cornell University

URL: http://hdl.handle.net/1813/10168

► The gastrointestinal (GI) tract is the largest surface exposed to the environment in the human body. Intestinal absorption is the entry route for most essential… (more)

▼ The gastrointestinal (GI) tract is the largest surface exposed to the environment in the human body. Intestinal absorption is the entry route for most essential nutrients and the preferred method for pharmaceutical administration. Understanding how compounds interact with and are absorbed through the small intestinal epithelium, and how they go on to affect the rest of the body, is crucial for nutritional and toxicological evaluation. Our group has developed an in vitro cell culture model of the gastrointestinal tract that includes digestion and physiologically realistic cell populations with a mucus layer. The applications of this model include predicting iron bioavailability, studying the effects of oral exposure to nanoparticles, and measuring the metabolism and toxicity of ingested chemicals or drugs. Co-cultures of absorptive and mucus-producing cells were incorporated into an existing in vitro digestion/cell culture model used to assess iron bioavailability. At physiologically relevant ratios of Caco-2 absorptive cells to HT29-MTX mucus-producing cells, a mucus layer completely covered the cell monolayer and the in vitro digestion model was nearly as responsive to changes in sample iron bioavailability as pure Caco-2 cultures. The existing in vitro digestion/Caco-2 cell culture model correlates well with human iron bioavailability studies, but, as mucus appears to play a role in iron absorption, the addition of a physiologically realistic mucus layer and goblet-type cells to this model may give more accurate iron bioavailability predictions. An in vitro cell culture model of the intestinal epithelium with absorptive, goblet, and M cells was used to study the effects of nanoparticle exposure on iron uptake and transport. Polystyrene 50 nm and 200 nm particles were found to interfere with cellular iron uptake and transport, indicating that oral exposure to nanoparticles could potentially have an effect on the normal physiological functions of intestinal epithelial cells. These preliminary results suggest that nanoparticle size, concentration, and charge can influence the uptake and transport of iron. Microscale cell culture analogs (microCCAs) are used to study the metabolism and toxicity of a chemical or drug. These in vitro devices are physical replicas of physiologically based pharmacokinetic models that combine microfabrication and cell culture. A GI tract microCCA, together with a multi-chamber silicon chip microCCA, has been used to recreate the toxic effects of acetaminophen. Proof of concept experiments show that acetaminophen passes through the in vitro intestinal epithelium and is metabolized by liver cells, resulting in liver cell toxicity.

Subjects/Keywords: gastrointestinal tract; in vitro

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APA (6^th Edition):

McAuliffe, G. (2008). Development of Physiologically Realistic In Vitro Models of the Gastrointestinal Tract . (Thesis). Cornell University. Retrieved from http://hdl.handle.net/1813/10168

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

McAuliffe, Gretchen. “Development of Physiologically Realistic In Vitro Models of the Gastrointestinal Tract .” 2008. Thesis, Cornell University. Accessed December 06, 2019. http://hdl.handle.net/1813/10168.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

McAuliffe, Gretchen. “Development of Physiologically Realistic In Vitro Models of the Gastrointestinal Tract .” 2008. Web. 06 Dec 2019.

Vancouver:

McAuliffe G. Development of Physiologically Realistic In Vitro Models of the Gastrointestinal Tract . [Internet] [Thesis]. Cornell University; 2008. [cited 2019 Dec 06]. Available from: http://hdl.handle.net/1813/10168.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

McAuliffe G. Development of Physiologically Realistic In Vitro Models of the Gastrointestinal Tract . [Thesis]. Cornell University; 2008. Available from: http://hdl.handle.net/1813/10168

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

24. Modin, Anna. En beskrivning av hur copingstrategier används av personer som har eller ska genomgå In Vitro Fertiliseringsbehandling, utifrån Lazarus och Folkmans teorier om coping : En kvalitativ studie.

Degree: Health and Caring Sciences, 2011, University of Gävle

URL: http://urn.kb.se/resolve?urn=urn:nbn:se:hig:diva-11193

► Syfte: Syftet med denna studie var att beskriva hur Lazarus och Folkmans copingstrategier används av kvinnor och män som genomgår IVF behandling, vid första… (more)

Subjects/Keywords: coping; In Vitro fertilisering; infertilitet

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APA (6^th Edition):

Modin, A. (2011). En beskrivning av hur copingstrategier används av personer som har eller ska genomgå In Vitro Fertiliseringsbehandling, utifrån Lazarus och Folkmans teorier om coping : En kvalitativ studie. (Thesis). University of Gävle. Retrieved from http://urn.kb.se/resolve?urn=urn:nbn:se:hig:diva-11193

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Modin, Anna. “En beskrivning av hur copingstrategier används av personer som har eller ska genomgå In Vitro Fertiliseringsbehandling, utifrån Lazarus och Folkmans teorier om coping : En kvalitativ studie.” 2011. Thesis, University of Gävle. Accessed December 06, 2019. http://urn.kb.se/resolve?urn=urn:nbn:se:hig:diva-11193.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Modin, Anna. “En beskrivning av hur copingstrategier används av personer som har eller ska genomgå In Vitro Fertiliseringsbehandling, utifrån Lazarus och Folkmans teorier om coping : En kvalitativ studie.” 2011. Web. 06 Dec 2019.

Vancouver:

Modin A. En beskrivning av hur copingstrategier används av personer som har eller ska genomgå In Vitro Fertiliseringsbehandling, utifrån Lazarus och Folkmans teorier om coping : En kvalitativ studie. [Internet] [Thesis]. University of Gävle; 2011. [cited 2019 Dec 06]. Available from: http://urn.kb.se/resolve?urn=urn:nbn:se:hig:diva-11193.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Modin A. En beskrivning av hur copingstrategier används av personer som har eller ska genomgå In Vitro Fertiliseringsbehandling, utifrån Lazarus och Folkmans teorier om coping : En kvalitativ studie. [Thesis]. University of Gävle; 2011. Available from: http://urn.kb.se/resolve?urn=urn:nbn:se:hig:diva-11193

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Delft University of Technology

25. Langeslag, S.A.E. Maturation Enhancement of Cardiac Myocytes In Vitro: Development of a Loading Protocol:.

Degree: 2011, Delft University of Technology

URL: http://resolver.tudelft.nl/uuid:31a47ae2-d68f-44cc-aeea-eede570702c1

► One of the major challenges in drug development is the development of a drug-screening model that closely resembles the adult human heart. Currently many drugs… (more)

▼ One of the major challenges in drug development is the development of a drug-screening model that closely resembles the adult human heart. Currently many drugs are rejected in late stages of development and even withdrawn from the market due to possible cardiac tissue damaging side effects. The corresponding delay in drug development is mainly due to a lack in drug-screening methods, to determine these life-threatening side effects. Cultivation of human cardiac cells in vitro could provide such a drug-screening model. Human cardiac muscle cells can be derived from human embryonic stem cells. However, the cardiac muscle cells derived by culturing these human embryonic stem cells into cardiomyocytes (cardiac muscle cells) appear to be immature in relation to cardiac muscle cells taken directly from the adult human body. This immaturity results in a drug-screening model with limited predictability. The hypothesis of the Cytostretch project, a collaborative project between Leiden University Medical Centre and Philips Research, is that in vitro cultured cardiomyocytes subjected to in vivo mimicking mechanical stimuli (stretch), will show enhanced maturation. These mature cell cultures provide a good basis for future drug-screening models. A chip is developed containing a polydimethylsiloxane (PDMS) thin-film as a substrate for cardiomyocyte culturing. A pressure difference is applied to the membrane to obtain a strained membrane, which with anchored cardiomyocytes will result in cardiomyocyte stretch. In order to stretch cardiac myocytes in vitro, the development of an in vivo mimicking loading protocol is essential. The main goal for the presented study, therefore, is the development of a proper loading protocol for in vitro stretching of cardiomyocytes. To reach this goal two study objectives have been defined; investigation of in vivo cardiac muscle strain during a normal cardiac cycle and the determination of the PDMS thin-film behavior. For the determination of the in vivo cardiac muscle strain, the left ventricle was modelled mathematically. Assumed was a homogeneous stress distribution along the left ventricular wall, corresponding to a fiber direction course along the wall, consistent with anatomical findings. The relation between left ventricular fiber stress and left ventricle pressure showed to depend mainly on the ratio of cavity volume over wall volume. The left ventricle mechanics can be approximated by: Where σf is the left ventricular fiber stress, Plv the left ventricular pressure. Vlv is the left ventricular cavity volume, Vw the left ventricular wall volume and Δεf the natural fiber strain. The outcome of the mathematical left ventricle model led to the conclusion that the absolute left ventricular fiber strain between end systole (reference volume) and end diastole equals an approximate 14.7% for a healthy adult human heart during normal cardiac cycle. The PDMS thin-film behavior is modelled analytically with use of classical thin plate mechanics, considering large deformations. The analytical derived… Advisors/Committee Members: Van Keulen, A..

Subjects/Keywords: Cardiac Myocytes; In Vitro

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APA (6^th Edition):

Langeslag, S. A. E. (2011). Maturation Enhancement of Cardiac Myocytes In Vitro: Development of a Loading Protocol:. (Masters Thesis). Delft University of Technology. Retrieved from http://resolver.tudelft.nl/uuid:31a47ae2-d68f-44cc-aeea-eede570702c1

Chicago Manual of Style (16^th Edition):

Langeslag, S A E. “Maturation Enhancement of Cardiac Myocytes In Vitro: Development of a Loading Protocol:.” 2011. Masters Thesis, Delft University of Technology. Accessed December 06, 2019. http://resolver.tudelft.nl/uuid:31a47ae2-d68f-44cc-aeea-eede570702c1.

MLA Handbook (7^th Edition):

Langeslag, S A E. “Maturation Enhancement of Cardiac Myocytes In Vitro: Development of a Loading Protocol:.” 2011. Web. 06 Dec 2019.

Vancouver:

Langeslag SAE. Maturation Enhancement of Cardiac Myocytes In Vitro: Development of a Loading Protocol:. [Internet] [Masters thesis]. Delft University of Technology; 2011. [cited 2019 Dec 06]. Available from: http://resolver.tudelft.nl/uuid:31a47ae2-d68f-44cc-aeea-eede570702c1.

Council of Science Editors:

Langeslag SAE. Maturation Enhancement of Cardiac Myocytes In Vitro: Development of a Loading Protocol:. [Masters Thesis]. Delft University of Technology; 2011. Available from: http://resolver.tudelft.nl/uuid:31a47ae2-d68f-44cc-aeea-eede570702c1

Texas A&M University

26. Klopatek, Sarah B. Ruminant Methanogenic Activity in the United States Beef Cattle Industry.

Degree: MS, Animal Science, 2016, Texas A&M University

URL: http://hdl.handle.net/1969.1/157878

► In the U.S., gastrointestinal fermentation from cattle is estimated to account for approximately 25% of total anthropogenic related methane (CH4) emissions. In addition, 2-8% of… (more)

▼ In the U.S., gastrointestinal fermentation from cattle is estimated to account for approximately 25% of total anthropogenic related methane (CH4) emissions. In addition, 2-8% of gross energy consumed by cattle is lost in the form of CH4, representing an energetic cost to the animal. Thereby, in order to decrease greenhouse gas emissions (GHG) and improve the efficiency of cattle production additional research on gastrointestinal CH4 emissions from cattle is needed. In ruminants carbohydrate (CHO) catabolism and nitrogen (N) utilization have a tremendous impact on ruminal methanogenesis. However, the impact of purified carbohydrates in the presence of a variety of N sources on rates of CH4 and VFA production remains unknown. In order to determine these rates for use in predictive models of the ruminal fermentation, we formulated a fractional rate equation to fit the rate of CH4 production and measured the concentration of CH4 and VFA and using purified CHO with a variety of N sources in two in vitro mixed ruminal microorganism fermentation studies. In both studies, a CHO treatment × incubation time (IT) effect was observed for both VFA and CH4 (P < 0.01). There was also an N × IT interaction for CH4 production at 24 h in Study 2, where nitrogen free and NH3 treatments produced greater concentrations of CH4 than treatments with amino acids (P < 0.01). A nonlinear equation for the conversion of carbohydrates to CH4 was able to fit starch treatments in Study 1 and glucose treatments in Study 2. Overall, this study demonstrated different fermentation patterns among all CHO and N sources and was the first step in determining rates for in vitro CH4 production. Although cattle contribute with high amounts of anthropologic GHG, they are not the only methanogenic producing food source in the U.S. Rice and wild ruminants (e.g. bison, elk, and deer) are also methanogenic producing food sources. The objective of this final study was to compare the efficiency of beef and milk production to pre-settlement wild ruminants and rice production on a kilogram of CH4 emitted to kilogram human-edible protein production basis. Bison had the highest ratio of 13.93 kg CH4: Protein, followed by elk (12.50) deer (6.66) and beef (2.47). Overall, wild ruminants emitted 296 to 564 percent more CH4 per kilogram of human-edible protein produced than current beef cattle production systems. Rice yielded the second lowest CH4 to human-edible protein ratio (0.83), followed by dairy cattle milk production (0.50). We believe, this analysis provides insight on the efficiency of methanogenic food sources that may aid in the development of regulatory guidelines of CH4 production. Advisors/Committee Members: Wickersham, Tryon (advisor), Tedeschi, Luis (advisor), Callaway, Todd (committee member), Sawyer, Jason (committee member).

Subjects/Keywords: Ruminants; Methane; in vitro; Sustainability

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Klopatek, S. B. (2016). Ruminant Methanogenic Activity in the United States Beef Cattle Industry. (Masters Thesis). Texas A&M University. Retrieved from http://hdl.handle.net/1969.1/157878

Chicago Manual of Style (16^th Edition):

Klopatek, Sarah B. “Ruminant Methanogenic Activity in the United States Beef Cattle Industry.” 2016. Masters Thesis, Texas A&M University. Accessed December 06, 2019. http://hdl.handle.net/1969.1/157878.

MLA Handbook (7^th Edition):

Klopatek, Sarah B. “Ruminant Methanogenic Activity in the United States Beef Cattle Industry.” 2016. Web. 06 Dec 2019.

Vancouver:

Klopatek SB. Ruminant Methanogenic Activity in the United States Beef Cattle Industry. [Internet] [Masters thesis]. Texas A&M University; 2016. [cited 2019 Dec 06]. Available from: http://hdl.handle.net/1969.1/157878.

Council of Science Editors:

Klopatek SB. Ruminant Methanogenic Activity in the United States Beef Cattle Industry. [Masters Thesis]. Texas A&M University; 2016. Available from: http://hdl.handle.net/1969.1/157878

Universidade Estadual de Campinas

27. Silva Neto, João Paulo da, 1985-. Avaliação da microinfiltração na interface pilar-implante .

Degree: 2012, Universidade Estadual de Campinas

URL: http://repositorio.unicamp.br/jspui/handle/REPOSIP/288293

► Resumo: A conexão entre implante e pilar está diretamente relacionada com o infiltrado bacteriano e a presença de células inflamatórias que levam à perda óssea… (more)

▼ Resumo: A conexão entre implante e pilar está diretamente relacionada com o infiltrado bacteriano e a presença de células inflamatórias que levam à perda óssea ao redor da microfenda formada por esta interface. Entretanto, ainda não há consenso quanto aos resultados dos testes in vitro e as metodologias aplicadas para a avaliação da microinfiltração nesta interface. O objetivo neste estudo foi: 1) avaliar, por meio de uma revisão sistemática, a influência das metodologias nos resultados dos estudos in vitro de microinfiltração na interface implante-pilar (I-P); 2) Avaliar, experimentalmente a microinfiltração bacteriana na interface I-P em implantes cone Morse inoculados com diferentes volumes de suspensão bacteriana. Para a revisão sistemática, foi realizada uma procura nas bases de dados MEDLINE, EMBASE e Cochrane, por estudos in vitro avaliando a microinfiltração na interface I-P publicados no período entre 1990 e agosto de 2011. Após a aplicação dos critérios de inclusão e exclusão, os artigos selecionados foram arranjados em tabelas e submetidos a análise descritiva. Para o ensaio de microinfiltração bacteriana foram selecionados implantes e pilares cone Morse, que por sua vez foram divididos em 2 grupos em função do tipo de pilar: parafuso passante (PP) e corpo sólido (CS). Posteriormente, os grupos foram subdivididos em 4 subgrupos, em função do volume de suspensão bacteriana inoculado nos implante (n=6): PP1: 0,1'mi'L; PP3: 0,3'mi'L; PP5: 0,5'mi'L; PP7: 0,7'mi'L; CS1: 0,1'mi'L; CS3: 0,3'mi'L; CS5: 0,5'mi'L e CS7: 0,7'mi'L. Uma suspensão bacteriana de Escherichia coli ATCC 35218 foi inoculada no interior dos implantes com volume de acordo com o respectivo grupo, sendo os componentes apertados segundo recomendações do fabricante. Após a inoculação os espécimes foram imersos em solução nutritiva bacteriana estéril para avaliação de um possível extravasamento do inoculo (grupo controle). Posteriormente, os conjuntos foram incubados em uma nova solução até o recobrimento da junção para avaliação microbiológica. A microinfiltração foi avaliada pela alteração na claridade da solução a cada 24 horas durante 7 dias. Ao final deste período os conjuntos foram reabertos para avaliação da viabilidade bacteriana. A revisão mostrou alta variabilidade de resultados e de tipos de ensaio de microinfiltração entre os estudos, apresentando alguns pontos críticos como volume bacteriano inoculado; concentração bacteriana; método de inoculação; períodos de acompanhamento e verificação de viabilidade bacteriana. No ensaio todos os espécimes inoculados com 0,7'mi'L e uma amostra de CS5 apresentaram turbidez no grupo controle após as primeiras 24 horas sendo excluídas do estudo. Durante os sete dias de acompanhamento nenhum espécime apresentou indicativo de microinfiltração bacteriana. E após este período, a viabilidade bacteriana foi confirmada em todos os espécimes avaliados. Dentro das limitações deste estudo pode se concluir que a falta de padronização entre os estudos in vitro dificulta comparações e pode explicar algumas… Advisors/Committee Members: Nóbilo, Mauro Antonio de Arruda, 1965- (advisor), Neves, Flávio Domingues das (advisor), Júnior, Paulo Cezar Simamoto (committee member), Ribeiro, Ricardo Faria (committee member), Henriques, Guilherme Elias Pessanha (committee member), Mesquita, Marcelo Ferraz (committee member).

Subjects/Keywords: Bactérias; In Vitro; Microbiologia

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APA (6^th Edition):

Silva Neto, João Paulo da, 1. (2012). Avaliação da microinfiltração na interface pilar-implante . (Thesis). Universidade Estadual de Campinas. Retrieved from http://repositorio.unicamp.br/jspui/handle/REPOSIP/288293

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Silva Neto, João Paulo da, 1985-. “Avaliação da microinfiltração na interface pilar-implante .” 2012. Thesis, Universidade Estadual de Campinas. Accessed December 06, 2019. http://repositorio.unicamp.br/jspui/handle/REPOSIP/288293.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Silva Neto, João Paulo da, 1985-. “Avaliação da microinfiltração na interface pilar-implante .” 2012. Web. 06 Dec 2019.

Vancouver:

Silva Neto, João Paulo da 1. Avaliação da microinfiltração na interface pilar-implante . [Internet] [Thesis]. Universidade Estadual de Campinas; 2012. [cited 2019 Dec 06]. Available from: http://repositorio.unicamp.br/jspui/handle/REPOSIP/288293.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Silva Neto, João Paulo da 1. Avaliação da microinfiltração na interface pilar-implante . [Thesis]. Universidade Estadual de Campinas; 2012. Available from: http://repositorio.unicamp.br/jspui/handle/REPOSIP/288293

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Minnesota

28. Stading, Amy. Development and Applications of In Vitro-Microdialysis: A Sampling Platform for Fast Analysis of Non-Electroactive Analytes.

Degree: PhD, Chemistry, 2016, University of Minnesota

URL: http://hdl.handle.net/11299/181667

► When considering the measurement of release events from cells, it can be done at levels as small as the single cell and performed in systems… (more)

▼ When considering the measurement of release events from cells, it can be done at levels as small as the single cell and performed in systems increasingly larger and more complex up to in vivo studies. Though in vitro systems lack the physiological relevance of in vivo, their simple and controlled environment is highly advantageous in preliminary mechanistic studies. In spite of this, there exists a serious gap in our ability to perform in vitro measurements on a wide array of analytes within a meaningful time frame. While electrochemical techniques are unparalleled in their ability to temporally resolve minute signals in biological systems, there is only a small class of targets which are suitable for this type of analysis. When analyzing non-electroactive analytes, measurements are often plagued by slow temporal responses (5+ minutes). Fluorescent imaging offers opportunities to monitor faster dynamics of non-electroactive analytes, but the target analyte must be either natively fluorescent or labeled, which can result in nonspecific binding and cytotoxicity. In both of these cases fast dynamics can be observed, but the array of analytes is small and only a few can be monitored simultaneously. In this work, a novel in vitro sampling platform is described which is capable of simultaneously monitoring approximately 15 non-electroactive analytes with 20 second temporal resolution. Cells were cultured on the surface of a microdialysis probe coupled with an analytical system for analysis. Small molecules released from the cells upon stimulation diffuse across the porous membrane because of the close proximity. A high-speed CE, built in house, enabled analysis of the collected dialysate. The ability of our platform to detect basal and stimulated release of amines was confirmed by transferring the probe between artificial cerebrospinal fluid (aCSF) and a potassium-spiked (100 mM K+-aCSF) stimulant solution. A variety of cell models were tested for compatibility with the in vitro-microdialysis platform, both single cell type and co-cultures were initiated. Adherence of viable cells was confirmed by labeling cells with either fluorescein diacetate (FDA) or specific antibody labelling, followed by imaging under a microscope. As a step towards continuously monitoring the change of non-electroactive analytes released from cultured cells, microdialysis was coupled directly to micro free flow electrophoresis (µFFE) device instead of the high-speed CE instrument.

Subjects/Keywords: Capillary Electrophoresis; In Vitro; Microdialysis

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Stading, A. (2016). Development and Applications of In Vitro-Microdialysis: A Sampling Platform for Fast Analysis of Non-Electroactive Analytes. (Doctoral Dissertation). University of Minnesota. Retrieved from http://hdl.handle.net/11299/181667

Chicago Manual of Style (16^th Edition):

Stading, Amy. “Development and Applications of In Vitro-Microdialysis: A Sampling Platform for Fast Analysis of Non-Electroactive Analytes.” 2016. Doctoral Dissertation, University of Minnesota. Accessed December 06, 2019. http://hdl.handle.net/11299/181667.

MLA Handbook (7^th Edition):

Stading, Amy. “Development and Applications of In Vitro-Microdialysis: A Sampling Platform for Fast Analysis of Non-Electroactive Analytes.” 2016. Web. 06 Dec 2019.

Vancouver:

Stading A. Development and Applications of In Vitro-Microdialysis: A Sampling Platform for Fast Analysis of Non-Electroactive Analytes. [Internet] [Doctoral dissertation]. University of Minnesota; 2016. [cited 2019 Dec 06]. Available from: http://hdl.handle.net/11299/181667.

Council of Science Editors:

Stading A. Development and Applications of In Vitro-Microdialysis: A Sampling Platform for Fast Analysis of Non-Electroactive Analytes. [Doctoral Dissertation]. University of Minnesota; 2016. Available from: http://hdl.handle.net/11299/181667

29. Kang, Hyeran. Kinetic and Kinematic Properties of Actin Network Assembly and Nonlinearity of Cross-Linked Gels.

Degree: PhD, Physics, 2009, Brown University

URL: https://repository.library.brown.edu/studio/item/bdr:232/

► Although recent studies have identified the biochemical components required to generate motile forces by actin polymerization, how the forces are generated remains unclear. To elucidate… (more)

▼ Although recent studies have identified the biochemical components required to generate motile forces by actin polymerization, how the forces are generated remains unclear. To elucidate this molecular mechanism of cell motility, I investigated the biophysical conditions under which actin-based growth and motility take place. In Part I of this dissertation, I report an experimental study on the kinetics of actin assembly mediated by branching and capping proteins. The experiments were performed by fluorescence imaging and light-scattering intensity measurement. The findings confirm the recent theoretical prediction that a "branching explosion" occurs during polymerization. Furthermore, the branching explosion occurs over a limited range of the ratio between branching protein and capping protein concentrations. This is also consistent with the theoretical model. These results establish a natural link between the kinetic theory of actin assembly in vitro and the cytoskeletal structure and actin dynamics in motile cells. In Part II of this dissertation, I present an in vitro study of the actin-based movement of functionalized spherical beads in comparison with those of bacteria like Listeria. Long trajectories induced by the spherical beads show characteristic differences with those observed for bacteria, which have both an elongated shape and an asymmetric expression of the polymerization inducing enzyme. The experimental trajectories can be simulated using a generalized kinematic model that includes the rotation of the bead relative to the actin tail. These results imply that the trajectories of spherical beads are mechanically deterministic rather than random, as suggested in stochastic models. In Part III of this dissertation, I examine nonlinear viscoelasticity of cross-linked biopolymer networks, by rheological measurements of shear moduli and normal stresses in fibrin gels. The results for coarse fibrin gels are consistent with expectations from theories of rod-like filament networks. Comparison of rheological and optical properties shows that the filament alignment, as measured by optical retardance increases with increasing shear strain but lags behind the increase in shear moduli. Advisors/Committee Members: Tang, Jay (director), Stein, Derek (reader), Shenoy, Vivek (reader), Shaw, Sunil (reader).

Subjects/Keywords: in vitro actin assembly

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APA (6^th Edition):

Kang, H. (2009). Kinetic and Kinematic Properties of Actin Network Assembly and Nonlinearity of Cross-Linked Gels. (Doctoral Dissertation). Brown University. Retrieved from https://repository.library.brown.edu/studio/item/bdr:232/

Chicago Manual of Style (16^th Edition):

Kang, Hyeran. “Kinetic and Kinematic Properties of Actin Network Assembly and Nonlinearity of Cross-Linked Gels.” 2009. Doctoral Dissertation, Brown University. Accessed December 06, 2019. https://repository.library.brown.edu/studio/item/bdr:232/.

MLA Handbook (7^th Edition):

Kang, Hyeran. “Kinetic and Kinematic Properties of Actin Network Assembly and Nonlinearity of Cross-Linked Gels.” 2009. Web. 06 Dec 2019.

Vancouver:

Kang H. Kinetic and Kinematic Properties of Actin Network Assembly and Nonlinearity of Cross-Linked Gels. [Internet] [Doctoral dissertation]. Brown University; 2009. [cited 2019 Dec 06]. Available from: https://repository.library.brown.edu/studio/item/bdr:232/.

Council of Science Editors:

Kang H. Kinetic and Kinematic Properties of Actin Network Assembly and Nonlinearity of Cross-Linked Gels. [Doctoral Dissertation]. Brown University; 2009. Available from: https://repository.library.brown.edu/studio/item/bdr:232/

30. Delplace, Céline. Microparticules à libération contrôlée : nouveaux polymères et importance des conditions de libération : Controlled release microparticles : novel polymers and insight into the importance of the release set-up.

Degree: Docteur es, Sciences du médicament, 2012, Université Lille II – Droit et Santé

URL: http://www.theses.fr/2012LIL2S007

► Les microparticules à base de copolymères d’acides lactique et glycolique (PLGA) sont biocompatibles et biodégradables tout en permettant de contrôler la libération des principes actifs… (more)

▼ Les microparticules à base de copolymères d’acides lactique et glycolique (PLGA) sont biocompatibles et biodégradables tout en permettant de contrôler la libération des principes actifs pendant quelques jours à plusieurs mois. Récemment, des stratégies ont été développées pour améliorer les propriétés de ces polymères en introduisant des groupements fonctionnels le long de la chaîne polymérique dans le but de moduler la libération des principes actifs.L’un des objectifs de ce travail a été d’étudier le potentiel de nouveaux copolymères fonctionnalisés portant des groupements carboxyliques pour la préparation de systèmes à libération contrôlée. L’apomorphine est encapsulée comme principe actif modèle. Son effet thérapeutique reste limité de part son court temps de demi-vie et son puissant effet émétique. Ainsi, des microparticules biodégradables, assurant une libération contrôlée de l’apomorphine, amélioreraient son efficacité thérapeutique et l’observance du traitement en réduisant la fréquence d’administration et les effets secondaires systémiques. Les microparticules chargées en apomorphine ont été préparées par une méthode d’émulsion à partir des nouveaux polymères fonctionnalisés, et de PLGA 50:50 de différentes masses moléculaires, pour comparaison. Les microparticules obtenues ont été caractérisées par différentes techniques. Le contenu résiduel en dichlorométhane (utilisé au cours de la formulation) a été quantifié et la libération de l’apomorphine a été étudiée in vitro. L’utilisation des polymères fonctionnalisés portant des fonctions carboxyliques libres a mené à une efficacité d’encapsulation plus élevée en apomorphine, de plus bas taux résiduels en dichlorométhane et à des cinétiques de libération in vitro de l’apomorphine distinctes de celles obtenues avec les PLGA traditionnels. Ces résultats suggèrent une application prometteuse de ces polymères fonctionnalisés pour la libération contrôlée de principes actifs. Une étape d’optimisation a ensuite consisté à modifier les paramètres de formulation afin d’étudier leur influence sur les caractéristiques des microparticules produites. L’objectif était notamment d’améliorer l’efficacité d’encapsulation tout en limitant la libération initiale d’apomorphine pouvant engendrer des pics de concentration néfastes, à l’origine d’effets indésirables. Ainsi, certains paramètres de formulation ont été modulés au cours de la préparation des microparticules à base de PLGA 50:50 de 10 kDa. La sélection de paramètres optimaux a mené au développement d’une formulation assurant une libération d’ordre zéro de l’apomorphine sur une période de dix jours.En outre, la littérature met en évidence l’importance des études de libération in vitro au cours du développement des microparticules de PLGA. Cependant, aucune méthode n’étant décrite par les autorités réglementaires, des conditions très différentes sont utilisées en pratique et leur influence sur les cinétiques de libération est peu connue. Par conséquent, une partie de ce travail a consisté à évaluer l’impact des conditions… Advisors/Committee Members: Siepmann, Juergen (thesis director), Siepmann, Florence (thesis director).

Subjects/Keywords: Microparticules; Essais in vitro

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Delplace, C. (2012). Microparticules à libération contrôlée : nouveaux polymères et importance des conditions de libération : Controlled release microparticles : novel polymers and insight into the importance of the release set-up. (Doctoral Dissertation). Université Lille II – Droit et Santé. Retrieved from http://www.theses.fr/2012LIL2S007

Chicago Manual of Style (16^th Edition):

Delplace, Céline. “Microparticules à libération contrôlée : nouveaux polymères et importance des conditions de libération : Controlled release microparticles : novel polymers and insight into the importance of the release set-up.” 2012. Doctoral Dissertation, Université Lille II – Droit et Santé. Accessed December 06, 2019. http://www.theses.fr/2012LIL2S007.

MLA Handbook (7^th Edition):

Delplace, Céline. “Microparticules à libération contrôlée : nouveaux polymères et importance des conditions de libération : Controlled release microparticles : novel polymers and insight into the importance of the release set-up.” 2012. Web. 06 Dec 2019.

Vancouver:

Delplace C. Microparticules à libération contrôlée : nouveaux polymères et importance des conditions de libération : Controlled release microparticles : novel polymers and insight into the importance of the release set-up. [Internet] [Doctoral dissertation]. Université Lille II – Droit et Santé 2012. [cited 2019 Dec 06]. Available from: http://www.theses.fr/2012LIL2S007.

Council of Science Editors:

Delplace C. Microparticules à libération contrôlée : nouveaux polymères et importance des conditions de libération : Controlled release microparticles : novel polymers and insight into the importance of the release set-up. [Doctoral Dissertation]. Université Lille II – Droit et Santé 2012. Available from: http://www.theses.fr/2012LIL2S007

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Open Access Theses and Dissertations