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You searched for subject:(Mitochondria Calcium Uniporter). One record found.

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1. Rysted, Jacob Eugene. Molecular mechanisms and functions of mitochondrial calcium transport in neurons.

Degree: PhD, Neuroscience, 2018, University of Iowa

During neuronal activity mitochondria alter cytosolic Ca2+ signaling by buffering then releasing Ca2+ in the cytosol. This calcium transport by mitochondria affects the amplitude, duration, and spacial profile of the Ca2+ signal in the cytosol of neurons. This buffering by mitochondria has been shown to affect a variety of neuronal functions including: neurotransmission, gene expression, cell excitability, and cell death. Recently, researchers discovered that the protein CCDC109A (mitochondrial Ca2+ uniporter) was the protein responsible for mitochondrial Ca2+ uptake. Using a genetic knockout (KO) mouse model for the mitochondrial Ca2+ uniporter (MCU) my research investigated the role of MCU in neuronal function. In cultured central and peripheral neurons, MCU-KO significantly reduced mitochondrial Ca2+ uptake while significantly increasing the amplitude of the cytosolic Ca2+ signal amplitude. Behaviorally, MCU-KO mice show a small but significant impairment in memory tasks: fear conditioning and Barnes maze. Using a maximal electroshock seizure threshold model of in vivo seizure activity my research found that MCU-KO significantly increases the threshold for maximal seizure activity in mice and significantly reduces seizure severity. In addition to mitochondrial Ca2+ uptake, my research also investigated the mechanisms involved in mitochondrial Ca2+ extrusion. The protein SLC8B1 (SLC24A6, NCLX) is the putative transporter responsible for the Na+/Ca2+ exchange, mitochondrial calcium extrusion. Using genetic NCLX-KO mice, our research found that in neurons NCLX contributes to cytosolic Ca2+ extrusion, but does seem to directly affect mitochondrial Ca2+ extrusion. Advisors/Committee Members: Usachev, Yuriy M. (supervisor).

Subjects/Keywords: calcium; mitochondria; mitochondrial calcium uniporter; NCLX; neuron; Neuroscience and Neurobiology

…x28;ccdc109b) MCUC: Mitochondrial calcium uniporter complex mPTP: mitochondrial… …PUBLIC ABSTRACT Mitochondria are known as the powerhouse of the cell, as they are the… …part of the cell that produces energy; however, alongside producing energy, mitochondria are… …survival; mitochondria have the ability to alter how much Ca2+ is inside the cell, how long it… …However, if too much Ca2+ enters the mitochondria this can lead to cell death, and this… 

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6th Edition):

Rysted, J. E. (2018). Molecular mechanisms and functions of mitochondrial calcium transport in neurons. (Doctoral Dissertation). University of Iowa. Retrieved from https://ir.uiowa.edu/etd/6632

Chicago Manual of Style (16th Edition):

Rysted, Jacob Eugene. “Molecular mechanisms and functions of mitochondrial calcium transport in neurons.” 2018. Doctoral Dissertation, University of Iowa. Accessed October 20, 2020. https://ir.uiowa.edu/etd/6632.

MLA Handbook (7th Edition):

Rysted, Jacob Eugene. “Molecular mechanisms and functions of mitochondrial calcium transport in neurons.” 2018. Web. 20 Oct 2020.

Vancouver:

Rysted JE. Molecular mechanisms and functions of mitochondrial calcium transport in neurons. [Internet] [Doctoral dissertation]. University of Iowa; 2018. [cited 2020 Oct 20]. Available from: https://ir.uiowa.edu/etd/6632.

Council of Science Editors:

Rysted JE. Molecular mechanisms and functions of mitochondrial calcium transport in neurons. [Doctoral Dissertation]. University of Iowa; 2018. Available from: https://ir.uiowa.edu/etd/6632

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