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You searched for subject:(Microstamping). Showing records 1 – 2 of 2 total matches.

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Clemson University

1. Bradley, Suzanne Nicole. Stretching Vascular Smooth Muscle Cells on Micropatterned Surfaces.

Degree: MS, Bioengineering, 2018, Clemson University

Vascular smooth muscle cells regulate blood flow by contracting and relaxing blood vessels. Vascular smooth muscle cells display one of two distinct phenotypes: contractile or synthetic. The contractile phenotype enables cells to contract, and the majority of cells in healthy blood vessels exhibit this phenotype. Transition to the synthetic phenotype is associated with abnormal mechanical forces and the development of vascular diseases, including hypertension and atherosclerosis. Cell-extracellular matrix interactions and mechanical stimulation are factors that affect phenotypic modulation. Micropatterning techniques create a microenvironment used to control cell morphology, adhesion, migration, proliferation, and differentiation. Vascular smooth muscle cells are circumferentially arranged in blood vessels, so parallel cell alignment mimics the native environment. Micropatterning vascular smooth muscle cells, either onto topographical features or extracellular matrix protein patterns, has been shown to promote a more contractile, in vivo-like phenotype. Vascular smooth muscle cells are exposed to continuous mechanical signaling from pulsatile blood flow, which enforces the contractile phenotype. Applying a physiologically relevant, cyclic stretching regimen to vascular smooth muscle cells also promotes contractile functioning. The purpose of this study was to determine the effects of micropatterning and mechanical stimulation on vascular smooth muscle cell phenotype. Three micropatterning techniques were implemented to determine the best method for aligning vascular smooth muscle cells. Microstamping was effective at patterning collagen but did not promote sufficient cell alignment. Stencil patterning vascular smooth muscle cells on a collagen layer was found to promote cell alignment with minimal cell spreading. Stenciled cells were exposed to physiological cyclic stretch using the MechanoCulture FX mechanical stimulation system (CellScale). The combination of stencil patterning and stretching reduced cell proliferation and promoted a more contractile phenotype. Advisors/Committee Members: Dr. Delphine Dean, Committee Chair, Dr. Bruce Gao, Dr. William Richardson.

Subjects/Keywords: Cell Stretching; Micropatterning; Microstamping; Vascular Smooth Muscle Cells

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APA (6th Edition):

Bradley, S. N. (2018). Stretching Vascular Smooth Muscle Cells on Micropatterned Surfaces. (Masters Thesis). Clemson University. Retrieved from https://tigerprints.clemson.edu/all_theses/2957

Chicago Manual of Style (16th Edition):

Bradley, Suzanne Nicole. “Stretching Vascular Smooth Muscle Cells on Micropatterned Surfaces.” 2018. Masters Thesis, Clemson University. Accessed August 19, 2019. https://tigerprints.clemson.edu/all_theses/2957.

MLA Handbook (7th Edition):

Bradley, Suzanne Nicole. “Stretching Vascular Smooth Muscle Cells on Micropatterned Surfaces.” 2018. Web. 19 Aug 2019.

Vancouver:

Bradley SN. Stretching Vascular Smooth Muscle Cells on Micropatterned Surfaces. [Internet] [Masters thesis]. Clemson University; 2018. [cited 2019 Aug 19]. Available from: https://tigerprints.clemson.edu/all_theses/2957.

Council of Science Editors:

Bradley SN. Stretching Vascular Smooth Muscle Cells on Micropatterned Surfaces. [Masters Thesis]. Clemson University; 2018. Available from: https://tigerprints.clemson.edu/all_theses/2957

2. WU LEI. Development and applications of hard microstamping.

Degree: 2004, National University of Singapore

Subjects/Keywords: microscale; micropatterning; hard microstamping; two-dimensional; three-dimensional; metal pattern

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6th Edition):

LEI, W. (2004). Development and applications of hard microstamping. (Thesis). National University of Singapore. Retrieved from http://scholarbank.nus.edu.sg/handle/10635/14192

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16th Edition):

LEI, WU. “Development and applications of hard microstamping.” 2004. Thesis, National University of Singapore. Accessed August 19, 2019. http://scholarbank.nus.edu.sg/handle/10635/14192.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7th Edition):

LEI, WU. “Development and applications of hard microstamping.” 2004. Web. 19 Aug 2019.

Vancouver:

LEI W. Development and applications of hard microstamping. [Internet] [Thesis]. National University of Singapore; 2004. [cited 2019 Aug 19]. Available from: http://scholarbank.nus.edu.sg/handle/10635/14192.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

LEI W. Development and applications of hard microstamping. [Thesis]. National University of Singapore; 2004. Available from: http://scholarbank.nus.edu.sg/handle/10635/14192

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

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