subject:(Microfluidic)

Oregon State University

1. Weymann, Davis. Properties of hydrogels for immobilization of bacteria in a commercial microfluidic dioreactor.

Degree: MS, 2017, Oregon State University

URL: http://hdl.handle.net/1957/61554

► Current technological shortcomings limit the economic viability of capturing and utilizing small sources of methane. The development of a reactor to overcome these limitations would… (more)

▼ Current technological shortcomings limit the economic viability of capturing and utilizing small sources of methane. The development of a reactor to overcome these limitations would unlock economic opportunity and incentivize reduced methane emissions. A microfluidic bioreactor containing immobilized methanotrophs has the potential to overcome these limitations by profitably converting small quantities of methane to more valuable liquid products. The material used for immobilizing bacteria in a microfluidic bioreactor must meet four criteria: biocompatibility, mechanical stability, reactor adhesion, and economic viability. This paper describes the development of a novel blend of agar and cross-linked polyvinyl alcohol (PVA) that meets these requirements. The properties of agar/PVA blend hydrogels strongly depend on the ratio and absolute concentration of the constituent polymers, and the processes by which the polymers are cross-linked. The microscopic morphology of these blend hydrogels is theorized to be two interacting and competing phases formed by agar and PVA molecules mutually interfering with cross-linking via hydrogen bonding, and separating due to spinodal decomposition. Evidence for the proposed morphology is discussed. Blend hydrogels of 2/5% agar/PVA are particularly promising, combining the desirable properties of both agar (low swelling) and PVA (strength and adhesion). The 2/5% agar/PVA gels exhibited little swelling in water and nitrate mineral salts-based media. They also adhered to polycarbonate and stainless steel surfaces treated with ozone or oxygen plasma. Cultures of Methylomicrobium buryatense 5GB1 (5GB1) immobilized in these gels showed a reduction of metabolic activity rates, partly due to exposure to high concentrations of sulfate and phosphate during cross-linking. Shortening cross-linker exposure time from 2 hours to 30 minutes greatly improved activity rates, and immobilized cells exhibited increased activity rates over time as fresh methane was added. Based on these results, the 2/5% agar/PVA blend hydrogels are suitable for the immobilization of 5GB1 in a microfluidic bioreactor. Further improvement of activity rates may be possible. Preservation of 5GB1 by lyophilization was unsuccessful. Cultures preserved in solutions of 5% bovine serum albumin and 10% sucrose or trehalose maintained metabolic activity rates after freezing at -80°C, but showed no activity after lyophilization. Advisors/Committee Members: Schilke, Karl (advisor), Jovanovic, Goran (committee member).

Subjects/Keywords: Microfluidic

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APA (6^th Edition):

Weymann, D. (2017). Properties of hydrogels for immobilization of bacteria in a commercial microfluidic dioreactor. (Masters Thesis). Oregon State University. Retrieved from http://hdl.handle.net/1957/61554

Chicago Manual of Style (16^th Edition):

Weymann, Davis. “Properties of hydrogels for immobilization of bacteria in a commercial microfluidic dioreactor.” 2017. Masters Thesis, Oregon State University. Accessed March 02, 2021. http://hdl.handle.net/1957/61554.

MLA Handbook (7^th Edition):

Weymann, Davis. “Properties of hydrogels for immobilization of bacteria in a commercial microfluidic dioreactor.” 2017. Web. 02 Mar 2021.

Vancouver:

Weymann D. Properties of hydrogels for immobilization of bacteria in a commercial microfluidic dioreactor. [Internet] [Masters thesis]. Oregon State University; 2017. [cited 2021 Mar 02]. Available from: http://hdl.handle.net/1957/61554.

Council of Science Editors:

Weymann D. Properties of hydrogels for immobilization of bacteria in a commercial microfluidic dioreactor. [Masters Thesis]. Oregon State University; 2017. Available from: http://hdl.handle.net/1957/61554

Texas A&M University

2. Sobahi, Nebras MohammedKamal A. Development of High-Throughput Microfluidic Impedance Spectroscopy Platform for Analyzing Microdroplets in Droplet Microfluidic System.

Degree: MS, Electrical Engineering, 2014, Texas A&M University

URL: http://hdl.handle.net/1969.1/153517

► This thesis presents the development of a high-throughput microfluidic impedance spectroscopy platform for electrically detecting analyzing impedance measurements of non-contact and label free microdroplets. This… (more)

Subjects/Keywords: microfluidic; impedance

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APA (6^th Edition):

Sobahi, N. M. A. (2014). Development of High-Throughput Microfluidic Impedance Spectroscopy Platform for Analyzing Microdroplets in Droplet Microfluidic System. (Masters Thesis). Texas A&M University. Retrieved from http://hdl.handle.net/1969.1/153517

Chicago Manual of Style (16^th Edition):

Sobahi, Nebras MohammedKamal A. “Development of High-Throughput Microfluidic Impedance Spectroscopy Platform for Analyzing Microdroplets in Droplet Microfluidic System.” 2014. Masters Thesis, Texas A&M University. Accessed March 02, 2021. http://hdl.handle.net/1969.1/153517.

MLA Handbook (7^th Edition):

Sobahi, Nebras MohammedKamal A. “Development of High-Throughput Microfluidic Impedance Spectroscopy Platform for Analyzing Microdroplets in Droplet Microfluidic System.” 2014. Web. 02 Mar 2021.

Vancouver:

Sobahi NMA. Development of High-Throughput Microfluidic Impedance Spectroscopy Platform for Analyzing Microdroplets in Droplet Microfluidic System. [Internet] [Masters thesis]. Texas A&M University; 2014. [cited 2021 Mar 02]. Available from: http://hdl.handle.net/1969.1/153517.

Council of Science Editors:

Sobahi NMA. Development of High-Throughput Microfluidic Impedance Spectroscopy Platform for Analyzing Microdroplets in Droplet Microfluidic System. [Masters Thesis]. Texas A&M University; 2014. Available from: http://hdl.handle.net/1969.1/153517

3. Thelwell, Jeray Anthony. Carrier bead systems in the microfluidic isolation of cfDNA for downstream analysis.

Degree: Biomedical Engineering, 2017, Brown University

URL: https://repository.library.brown.edu/studio/item/bdr:733538/

► The field of medical diagnostics is governed by two practical goals that drive the engineering and relevant innovation necessary to move the technology forward. Firstly,… (more)

▼ The field of medical diagnostics is governed by two practical goals that drive the engineering and relevant innovation necessary to move the technology forward. Firstly, diagnostic technology must have predictive potential. As medical education continues to develop with the growing body of basic biological research, there is increased emphasis on preventative medicine. Instead of training more highly skilled surgical specialty doctors to mechanically remedy exacerbated ailments of the body, lives are now being saved before patients even make it into the operating room. Predictive diagnostic technologies provide a wealth of information, not only about current pathological status, but also about the real-time effects of treatment with little to no harm of the patient. Secondly, diagnostic technology must offer personalized patient information. With completion of the human genome project and the advent of more sophisticated sequencing modalities, medical care tailored to specific individuals is more of a possibility. A greater understanding of epigenetic modulation of the genome is promoting the transition from “one size fits all” care. While approaching this problem in the Tripathi Lab, two additional constraints were imposed: the diagnostic technology must be robust yet economical and simple to use in order to allow for widespread adoption. To that end, a microfluidic platform for the extraction of short fragment DNA from crowded solution is reported with the idea of mimicking cell-free DNA found in blood. Preliminary off-chip experiments comparing different magnetic beads to determine the optimal bead and buffer composition for short DNA fragments are first completed. Then an investigation into multi-bead systems for size based extraction through the oil-water interface is carried out. Finally, on-chip short fragment DNA recovery is demonstrated from buffer solution and spiked human serum solution. Advisors/Committee Members: Tripathi, Anubhav (Advisor), Breuer, Kenneth (Reader), Mathiowitz, Edith (Reader), Wessel, Gary (Reader).

Subjects/Keywords: Microfluidic devices

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APA (6^th Edition):

Thelwell, J. A. (2017). Carrier bead systems in the microfluidic isolation of cfDNA for downstream analysis. (Thesis). Brown University. Retrieved from https://repository.library.brown.edu/studio/item/bdr:733538/

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Thelwell, Jeray Anthony. “Carrier bead systems in the microfluidic isolation of cfDNA for downstream analysis.” 2017. Thesis, Brown University. Accessed March 02, 2021. https://repository.library.brown.edu/studio/item/bdr:733538/.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Thelwell, Jeray Anthony. “Carrier bead systems in the microfluidic isolation of cfDNA for downstream analysis.” 2017. Web. 02 Mar 2021.

Vancouver:

Thelwell JA. Carrier bead systems in the microfluidic isolation of cfDNA for downstream analysis. [Internet] [Thesis]. Brown University; 2017. [cited 2021 Mar 02]. Available from: https://repository.library.brown.edu/studio/item/bdr:733538/.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Thelwell JA. Carrier bead systems in the microfluidic isolation of cfDNA for downstream analysis. [Thesis]. Brown University; 2017. Available from: https://repository.library.brown.edu/studio/item/bdr:733538/

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Oregon State University

4. Rundel, Jack T. Design, fabrication and application of a microfluidic nanofiltration module for separation and purification of macromolecules and nanoparticles.

Degree: PhD, Chemistry, 2008, Oregon State University

URL: http://hdl.handle.net/1957/7682

► A microfluidic nanofiltration module has been designed, fabricated and applied to the continuous-flow, pressure-driven, post-synthetic purification of macromolecules and nanoparticles via diafiltration using a commercially… (more)

▼ A microfluidic nanofiltration module has been designed, fabricated and applied to the continuous-flow, pressure-driven, post-synthetic purification of macromolecules and nanoparticles via diafiltration using a commercially available organic solvent resistant nanofiltration membrane, STARMEM 122. This module will readily interface with other microscale components within a "nanofactory" for the rapid synthesis, purification and delivery of highly dispersed macromolecules and nanoparticles. The microfluidic nanofiltration module (MNM) fully integrates the membrane into an all-polymer format constructed of materials that are relatively inexpensive, chemically compatible with the targeted compounds and transmissive to visible light allowing optical monitoring of the system. A molecular weight cutoff of 2.3 kDa was determined for the MNM using half-generation poly(amidoamine) dendrimer standards in a simulated postsynthetic mixture of 4 mM methyl acrylate in methanol. The membrane was also characterized within a macroscale test fixture (MTF), constructed of reusable parts that are easily disassembled for rapid replacement of the membrane. Rejections of 95% to 97% were observed in the MTF for triphenylphosphine (PPh3) stabilized gold-eleven (Au11) nanoparticle standards. Rejections of 12% to 58% were also observed for PPh₃, an anticipated post-synthetic byproduct. Purification via diafiltration of realworld post-synthetic Au₁₁ mixtures was demonstrated in the MTF. Au₁₁ rejections > 99% were observed along with expected decreases in PPh₃ concentrations. Stable permeances of 2 L m⁻² h⁻¹ bar⁻¹ were also observed, comparable to those reported in the literature and claimed by the membrane manufacturer. The purity of the Au₁₁ final product was comparable to that of the crystalline Au11 standard purified by traditional means. These results suggest that a diafiltration system incorporating an organic solvent resistant nanofiltration membrane would be practical for rapid purification and high product recovery of gold nanoparticles in an organic solvent environment immediately downstream of a microreactor within a nanofactory architecture. Advisors/Committee Members: Remcho, Vincent T (advisor), Paul, Brian K (committee member).

Subjects/Keywords: microfluidic; Microfluidics

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APA (6^th Edition):

Rundel, J. T. (2008). Design, fabrication and application of a microfluidic nanofiltration module for separation and purification of macromolecules and nanoparticles. (Doctoral Dissertation). Oregon State University. Retrieved from http://hdl.handle.net/1957/7682

Chicago Manual of Style (16^th Edition):

Rundel, Jack T. “Design, fabrication and application of a microfluidic nanofiltration module for separation and purification of macromolecules and nanoparticles.” 2008. Doctoral Dissertation, Oregon State University. Accessed March 02, 2021. http://hdl.handle.net/1957/7682.

MLA Handbook (7^th Edition):

Rundel, Jack T. “Design, fabrication and application of a microfluidic nanofiltration module for separation and purification of macromolecules and nanoparticles.” 2008. Web. 02 Mar 2021.

Vancouver:

Rundel JT. Design, fabrication and application of a microfluidic nanofiltration module for separation and purification of macromolecules and nanoparticles. [Internet] [Doctoral dissertation]. Oregon State University; 2008. [cited 2021 Mar 02]. Available from: http://hdl.handle.net/1957/7682.

Council of Science Editors:

Rundel JT. Design, fabrication and application of a microfluidic nanofiltration module for separation and purification of macromolecules and nanoparticles. [Doctoral Dissertation]. Oregon State University; 2008. Available from: http://hdl.handle.net/1957/7682

University of KwaZulu-Natal

5. Moodley, Revesa Sadasivan. Evaluation of paper substrates for microfluidic application in medical diagnostic kits.

Degree: 2018, University of KwaZulu-Natal

URL: https://researchspace.ukzn.ac.za/handle/10413/16613

► Recent studies in the biomedical field have shown the use of paper in microfluidic analytical devices. However, no studies have been undertaken to ascertain what… (more)

▼ Recent studies in the biomedical field have shown the use of paper in microfluidic analytical devices. However, no studies have been undertaken to ascertain what type(s) of paper substrates are ideal for such microfluidic applications. Hence, this study was conducted to determine the feasibility of using different paper substrates for implementation in microfluidic analytical devices, specifically in the South African context, compared to currently used materials such as glass and silicone. In addition, fibres in paper substrates were substituted with nanofibrillated cellulose (NFC) fibres to ascertain the impact of NFC on the microfluidics of the paper substrates. The wicking rate of the substrates was the focus of the study, with high -resolution field emission gun-scanning electron microscopy and contact angle tests being used to support the results obtained. Solid wax printing was also conducted to determine whether the paper substrates were suited to fabrication for microfluidic applications. High-resolution morphological studies of the paper substrates showed that pore sizes of the pulp fibres were in the order sulphite> bleached kraft > unbleached kraft > Whatman No. 1 Chromatography Filter Paper > Whatman 3MM Chromatography Filter Paper > thermo-mechanical > recycled. It was concluded that the larger pore size of fibres correlated with faster wicking rates of the relevant paper substrates. The substitution of pulp fibres with NFC led to reduced pore size of the fibres thus leading to reduced wicking rates due to the presence of the small NFC particles. Contact angle is directly linked to the hydrophobicity of the substrate and is indicative of the resistance to absorption of a liquid. The results revealed that all the paper substrates were hydrophilic. However, the hydrophilicity of two of the substrates (sheets substituted with 100% NFC unbleached kraft pulp and 100% NFC recycled pulp) were higher than those of the other substrates indicating that, although these substrates were still hydrophilic in nature, their absorption of aqueous liquid would take longer periods of time. The results showed that Whatman glass microfiber GF/D filter paper was the fastest wicking substrate, using both dye and blood simulant as wicking liquids. Similarly, paper substrates made with recycled fibres exhibited the slowest wicking rates when using both wicking liquids. These results can be used when determining which of the substrates to use for paper-based microfluidic device (μPAD) application, whereby the desired detection time would be the factor used to establish which of the substrates to use. Comparison of vertical and horizontal tests showed varying results. In theory, the horizontal wicking test should result in a faster wicking rate than the vertical test, taking hydrostatic pressure into consideration. The majority of the substrates showed that the horizontal wicking rate was faster when using the dye solution, whereas vertical wicking was faster when using the blood simulant. Discrepancies between the results… Advisors/Committee Members: Land, Kevin. (advisor), Sithole, Bishop Bruce. (advisor), Van Zyl, Werner Ewald. (advisor), Andrew, Jerome. (advisor).

Subjects/Keywords: MICROFLUIDIC.; DIAGNOSTIC.

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APA (6^th Edition):

Moodley, R. S. (2018). Evaluation of paper substrates for microfluidic application in medical diagnostic kits. (Thesis). University of KwaZulu-Natal. Retrieved from https://researchspace.ukzn.ac.za/handle/10413/16613

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Moodley, Revesa Sadasivan. “Evaluation of paper substrates for microfluidic application in medical diagnostic kits.” 2018. Thesis, University of KwaZulu-Natal. Accessed March 02, 2021. https://researchspace.ukzn.ac.za/handle/10413/16613.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Moodley, Revesa Sadasivan. “Evaluation of paper substrates for microfluidic application in medical diagnostic kits.” 2018. Web. 02 Mar 2021.

Vancouver:

Moodley RS. Evaluation of paper substrates for microfluidic application in medical diagnostic kits. [Internet] [Thesis]. University of KwaZulu-Natal; 2018. [cited 2021 Mar 02]. Available from: https://researchspace.ukzn.ac.za/handle/10413/16613.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Moodley RS. Evaluation of paper substrates for microfluidic application in medical diagnostic kits. [Thesis]. University of KwaZulu-Natal; 2018. Available from: https://researchspace.ukzn.ac.za/handle/10413/16613

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Rutgers University

6. Prakash, Shreya, 1994-. A microfluidic biosensing architecture for multiplexed analyte detection using hydrogel barcoded particles.

Degree: MS, Electrical and Computer Engineering, 2019, Rutgers University

URL: https://rucore.libraries.rutgers.edu/rutgers-lib/61911/

► Multiplexing is a method of analyzing multiple analytes in a biological assay in a single step. It provides advantage of shorter processing time, low sample… (more)

▼ Multiplexing is a method of analyzing multiple analytes in a biological assay in a single step. It provides advantage of shorter processing time, low sample volume and reduced cost per test. Currently, Flow Cytometers are used for blood cells enumeration, however, they are expensive, requires fluorescent tagging of cells and trained technicians to operate the instrument. We propose a non-fluorescent microfluidic architecture with single excitation and detection scheme using the barcoded particles fabricated using Stop Flow Lithography process. The barcoded particles designed with specific number of coding regions can generate numerous distinct patterns of electrical signatures and can be used in biological assays to detect multiple blood cells. A novel design of the asymmetric rectangular barcoded particle was proposed and tested in COMSOL Multiphysics. The barcoded particle with five coding regions generated distinct bi-polar signatures in the microfluidic impedance detection system. Different configurations of the sensing system were proposed to detect the conjugated microspheres (representative of blood cells) effectively based on the site of conjugation. The bottom co-planar electrode and top-bottom electrode configurations were tested for sensitive detection of conjugated microspheres to the barcoded particle. We found that our microfluidic detection system was sensitive enough to detect the presence of a microsphere attached to the barcoded particle. Further, we also investigated the effect of microspheres conjugation orientation to the barcoded particle and their associated electrical signatures. We developed a multi-feature selection algorithm to solve the orientation problem and improved the accuracy of our sensing mechanism. Finally, we fabricated the microfluidic chips using lithography, and co-planar electrodes on glass surfaces using thin film deposition process in the clean room. Our proposed microfluidic system can enumerate multiple blood cells in a single assay using barcoded particles. The concentration of these cells provides useful information about disease onset and progression. Such sensors can be used for diagnostic and management of diseases like Sepsis, Acute Kidney Injury, HIV/ AIDS. Advisors/Committee Members: Hassan, Umer (chair), Gajic, Zoran (internal member), Wu, Chung-Tse Michael (internal member), School of Graduate Studies.

Subjects/Keywords: Microfluidic; Biosensors

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APA (6^th Edition):

Prakash, Shreya, 1. (2019). A microfluidic biosensing architecture for multiplexed analyte detection using hydrogel barcoded particles. (Masters Thesis). Rutgers University. Retrieved from https://rucore.libraries.rutgers.edu/rutgers-lib/61911/

Chicago Manual of Style (16^th Edition):

Prakash, Shreya, 1994-. “A microfluidic biosensing architecture for multiplexed analyte detection using hydrogel barcoded particles.” 2019. Masters Thesis, Rutgers University. Accessed March 02, 2021. https://rucore.libraries.rutgers.edu/rutgers-lib/61911/.

MLA Handbook (7^th Edition):

Prakash, Shreya, 1994-. “A microfluidic biosensing architecture for multiplexed analyte detection using hydrogel barcoded particles.” 2019. Web. 02 Mar 2021.

Vancouver:

Prakash, Shreya 1. A microfluidic biosensing architecture for multiplexed analyte detection using hydrogel barcoded particles. [Internet] [Masters thesis]. Rutgers University; 2019. [cited 2021 Mar 02]. Available from: https://rucore.libraries.rutgers.edu/rutgers-lib/61911/.

Council of Science Editors:

Prakash, Shreya 1. A microfluidic biosensing architecture for multiplexed analyte detection using hydrogel barcoded particles. [Masters Thesis]. Rutgers University; 2019. Available from: https://rucore.libraries.rutgers.edu/rutgers-lib/61911/

Oregon State University

7. Koesdjojo, Myra T. Fabrication and application of polymer based microfluidic devices.

Degree: PhD, Chemistry, 2009, Oregon State University

URL: http://hdl.handle.net/1957/10841

► The concept of reducing laboratory operations in scale such that they fit on a microfluidic chip has been met with great enthusiasm. Lab-on-a-chip devices promise… (more)

▼ The concept of reducing laboratory operations in scale such that they fit on a microfluidic chip has been met with great enthusiasm. Lab-on-a-chip devices promise to be cost effective to operate due to reduced reagent consumption, have the potential to offer shorter analysis times due to their short path lengths, and may be useful in biological applications in that they are inherently compact and inexpensive to build, thus they may be disposable. In this work, a series of fabrication techniques for the production of polymer-based microfluidic devices are explored. In the first component of this research effort an aluminum mold was fabricated using CNC machining to create the desired microchannel design, followed by a two-stage embossing process, involving two polymer substrates with different glass transition temperatures (Tg), polyetherimide (PEI; Tg~216 °C) and poly(methyl methacrylate) (PMMA; Tg~105 °C). Successful feature transfer from aluminum mold to PMMA substrates was achieved reproducibly employing this method. With this approach, the expensive process of producing the aluminum master need be performed only once. Electrophoretic separations of fluorescent dyes, rhodamine B and fluorescein were performed on the PMMA microchips, with peak efficiencies of 55500 and 66300 theoretical plates/meter, respectively. The next stage of work explored a new bonding method by solvent welding using ice as sacrificial layer to prevent channel deformation. Water is one of the most compatible sacrificial media; it is readily available, non toxic, has a low evaporation rate, a high freezing point relative to the bonding solvent, and a low melting point which makes it easier to flush out after sealing, as compared to using other sacrificial media (paraffin wax or low-melting temperature alloys). The bonded PMMA microchips could withstand an internal pressure of > 2000 psi, more than 17 times stronger than the thermally bonded chips. In the final stage of work a new bonding technique was developed that readily produces complete microfluidic chips, without the need of a sacrificial layer to form complete multilayer microfluidic devices. Also developed was the use of an SU-8 master in the two-stage embossing process to create microchannels. This approach is faster, simpler and less costly than CNC machining. The fabrication technique was utilized to build a microfluidic liquid chromatography (LC) system that was shown to generate high separation efficiencies of 10,000- 45,000 plates/m. In addition, a passive micromixer containing high-density microfeatures was fabricated to perform a glycine assay using O-phthaldialdehyde. With glycine concentrations ranging from 0.0 to 2.6 μM, a linear calibration plot (R2 = 0.9982) was obtained with a detection limit of 0.032 μM. Advisors/Committee Members: Remcho, Vincent (advisor), Chang, Chih-hung (committee member).

Subjects/Keywords: microfluidic; Microfluidic devices – Design and construction

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APA (6^th Edition):

Koesdjojo, M. T. (2009). Fabrication and application of polymer based microfluidic devices. (Doctoral Dissertation). Oregon State University. Retrieved from http://hdl.handle.net/1957/10841

Chicago Manual of Style (16^th Edition):

Koesdjojo, Myra T. “Fabrication and application of polymer based microfluidic devices.” 2009. Doctoral Dissertation, Oregon State University. Accessed March 02, 2021. http://hdl.handle.net/1957/10841.

MLA Handbook (7^th Edition):

Koesdjojo, Myra T. “Fabrication and application of polymer based microfluidic devices.” 2009. Web. 02 Mar 2021.

Vancouver:

Koesdjojo MT. Fabrication and application of polymer based microfluidic devices. [Internet] [Doctoral dissertation]. Oregon State University; 2009. [cited 2021 Mar 02]. Available from: http://hdl.handle.net/1957/10841.

Council of Science Editors:

Koesdjojo MT. Fabrication and application of polymer based microfluidic devices. [Doctoral Dissertation]. Oregon State University; 2009. Available from: http://hdl.handle.net/1957/10841

Cornell University

8. Santana, Steven. Microfluidic Analysis Of Metastatic Cancer Biomarkers.

Degree: PhD, Mechanical Engineering, 2014, Cornell University

URL: http://hdl.handle.net/1813/38925

► Cancer is the second-leading cause of death in the United States. Metastasis is responsible for 90% of cancer-related death and progresses through multifarious, poorly-understood cascades… (more)

▼ Cancer is the second-leading cause of death in the United States. Metastasis is responsible for 90% of cancer-related death and progresses through multifarious, poorly-understood cascades as they are difficult to observe in vivo. It is widely held that deciphering the metastatic cascade and identifying metastatic precursors will lead to improved patient outcomes. In this work, we isolate and study cancer biomarkers, specifically circulating tumor cells (CTCs) and cancer-cell-derived extracellular shed vesicles (ESVs), implicated in cancer progression and metastasis. First we describe the design, fabrication, and use of a Hele-Shaw microfluidic system to optimize rarecell immunocapture parameters with a focus on informing the design of systems for CTC isolation from patient whole blood. Our study includes the role of antibody selection, density, antigen locations, and multi-modal capture surfaces, as well as shear stress, on rare cell capture. We use LNCaPs, a PSMA-expressing prostate cancer cell line, as a model for CTCs and anti-PSMA antibodies, J591 and J415, to inform chemistry-mediated immobilization. Next, we focus on another cancer-disseminated marker, extracellular shed vesicles. ESVs, including exosomes and cancer-cell-derived microvesicles, are disseminated throughout the body and represent an important conduit of cell communication. Microvesicles have potential as a cancer biomarker as they are believed to transform tumor microenvironments and prime the metastatic niche. Cancercell-derived ESV subpopulations consist of a small-diameter exosome population and a large-diameter, cancer-cell-specific microvesicle population, each formed by unique mechanisms. It is believed that size correlates with biological properties of interest, but isolating these subpopulations, to discern chemical, biological, or physical differences, is challenging. We designed a deterministic lateral displacement microfluidic platform to isolate a pure microvesicle sample from the heterogeneous cancer-cell-derived ESV population. The threshold diameter differentiating the microvesicle population from the exosome population was determined by characterizing the size distributions of ESVs harvested from multiple cancer cell lines of breast, brain, and pancreas origin. Our microvesicle-isolation microfluidic technology facilitates future investigations regarding microvesicles' role in cancer progression by enabling identification of cargo carried by the microvesicle subpopulation. Advisors/Committee Members: Kirby, Brian (chair), Fischbach, Claudia (committee member), Sipple, John W (committee member), Cerione, Richard A (committee member).

Subjects/Keywords: microfluidic; metastasis; biomarker

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APA (6^th Edition):

Santana, S. (2014). Microfluidic Analysis Of Metastatic Cancer Biomarkers. (Doctoral Dissertation). Cornell University. Retrieved from http://hdl.handle.net/1813/38925

Chicago Manual of Style (16^th Edition):

Santana, Steven. “Microfluidic Analysis Of Metastatic Cancer Biomarkers.” 2014. Doctoral Dissertation, Cornell University. Accessed March 02, 2021. http://hdl.handle.net/1813/38925.

MLA Handbook (7^th Edition):

Santana, Steven. “Microfluidic Analysis Of Metastatic Cancer Biomarkers.” 2014. Web. 02 Mar 2021.

Vancouver:

Santana S. Microfluidic Analysis Of Metastatic Cancer Biomarkers. [Internet] [Doctoral dissertation]. Cornell University; 2014. [cited 2021 Mar 02]. Available from: http://hdl.handle.net/1813/38925.

Council of Science Editors:

Santana S. Microfluidic Analysis Of Metastatic Cancer Biomarkers. [Doctoral Dissertation]. Cornell University; 2014. Available from: http://hdl.handle.net/1813/38925

University of Wisconsin – Milwaukee

9. Amin, Alaknanda. Microfluidic Device Integration of Electrostatic Corral Trapping Systems.

Degree: MS, Chemistry, 2014, University of Wisconsin – Milwaukee

URL: https://dc.uwm.edu/etd/519

► This thesis describes the development, characterization, and application of the microfluidics device integration of electrostatic corral trapping systems. Optical traps or "laser tweezers", which… (more)

Subjects/Keywords: Microfluidic; Trapping; Chemistry

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APA (6^th Edition):

Amin, A. (2014). Microfluidic Device Integration of Electrostatic Corral Trapping Systems. (Thesis). University of Wisconsin – Milwaukee. Retrieved from https://dc.uwm.edu/etd/519

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Amin, Alaknanda. “Microfluidic Device Integration of Electrostatic Corral Trapping Systems.” 2014. Thesis, University of Wisconsin – Milwaukee. Accessed March 02, 2021. https://dc.uwm.edu/etd/519.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Amin, Alaknanda. “Microfluidic Device Integration of Electrostatic Corral Trapping Systems.” 2014. Web. 02 Mar 2021.

Vancouver:

Amin A. Microfluidic Device Integration of Electrostatic Corral Trapping Systems. [Internet] [Thesis]. University of Wisconsin – Milwaukee; 2014. [cited 2021 Mar 02]. Available from: https://dc.uwm.edu/etd/519.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Amin A. Microfluidic Device Integration of Electrostatic Corral Trapping Systems. [Thesis]. University of Wisconsin – Milwaukee; 2014. Available from: https://dc.uwm.edu/etd/519

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Oregon State University

10. Tennico, Yolanda H. Magnetic particles for selective extraction of trace analytes in microfluidic devices.

Degree: PhD, Chemistry, 2010, Oregon State University

URL: http://hdl.handle.net/1957/14783

► The development of micro total analysis systems (µTAS), also called “lab-on-a-chip”, or microfluidic analysis systems, is presented in this dissertation. Various research areas, covering subjects… (more)

▼ The development of micro total analysis systems (µTAS), also called “lab-on-a-chip”, or microfluidic analysis systems, is presented in this dissertation. Various research areas, covering subjects from magnetic particles synthesis to novel microchip fabrication techniques, are explored to develop a lab-on-a-chip system capable of performing magnetic bead-based bioassays. These devices are proven not only to be cost-effective (due to their reduced reagent consumption), but also time-efficient (shorter analysis time), and may be disposable (they are inherently compact and inexpensive to produce), making them very attractive to be used in biomedical applications. The research starts with the utilization of functionalized magnetic particles as sorbents for an in-line extraction in confines of capillary columns. The synthesized silica-coated iron oxide particles functionalized with C18 groups were used as reversed-phase sorbents. Magnets were used to locally immobilize these particles inside the capillary. The results showed that extraction, elution, and detection of the analytes were performed sequentially without interruption or need for sample handling. The work continued with the development of bonding techniques for creating polymer-based microfluidic devices through surface modification process. This technique readily produced complete microfluidic chips via plasma oxidation followed by silane reagent treatment (tetraethyl orthosilicate) to facilitate siloxane bonds between the two polymers. It provides a versatile approach to bond dissimilar polymer substrates and between a hard polymer substrate to polydimethylsiloxane or glass. The two previous studies were combined allowing for the integration of magnetic particles in a microfluidic chip format. The benefits of each component were utilized to develop an on-chip aptamer-based fluorescence assay for thrombin detection and quantification based on sandwich ELISA principles. Aptamer-functionalized magnetic beads were utilized to capture the target analyte, while a second aptamer, functionalized with quantum dots, was employed for on-chip detection. Disposable microfluidic devices were employed as the platform to enable rapid and sensitive thrombin detection with the benefits of reduced costs, minimized material consumption, and simplified washing process. This work has opened up a new chapter in the development of magnetic beads-based materials and devices in a wide variety of applications for detection of target proteins of biomedical importance. Advisors/Committee Members: Remcho, Vincent T. (advisor), Lerner, Michael (committee member).

Subjects/Keywords: microfluidics; Microfluidic devices

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Tennico, Y. H. (2010). Magnetic particles for selective extraction of trace analytes in microfluidic devices. (Doctoral Dissertation). Oregon State University. Retrieved from http://hdl.handle.net/1957/14783

Chicago Manual of Style (16^th Edition):

Tennico, Yolanda H. “Magnetic particles for selective extraction of trace analytes in microfluidic devices.” 2010. Doctoral Dissertation, Oregon State University. Accessed March 02, 2021. http://hdl.handle.net/1957/14783.

MLA Handbook (7^th Edition):

Tennico, Yolanda H. “Magnetic particles for selective extraction of trace analytes in microfluidic devices.” 2010. Web. 02 Mar 2021.

Vancouver:

Tennico YH. Magnetic particles for selective extraction of trace analytes in microfluidic devices. [Internet] [Doctoral dissertation]. Oregon State University; 2010. [cited 2021 Mar 02]. Available from: http://hdl.handle.net/1957/14783.

Council of Science Editors:

Tennico YH. Magnetic particles for selective extraction of trace analytes in microfluidic devices. [Doctoral Dissertation]. Oregon State University; 2010. Available from: http://hdl.handle.net/1957/14783

Oregon State University

11. Nammoonnoy, Jintana. A photoactivable microfluidic device for heavy metal ion extraction.

Degree: PhD, Chemistry, 2010, Oregon State University

URL: http://hdl.handle.net/1957/18461

► The development of microfluidic devices for heavy metal extraction is presented in this dissertation. Various research areas, covering subjects from photochromic compound syntheses to microchip… (more)

▼ The development of microfluidic devices for heavy metal extraction is presented in this dissertation. Various research areas, covering subjects from photochromic compound syntheses to microchip fabrication techniques are explored to develop microfluidic devices capable of extracting heavy metal ions from drinking water. Through integration of the beneficial characteristics of both microfluidic devices and photochromic dyes, a simple and innovative microchip configured as a photoactivable extraction system for metal ion accumulation and release can be realized. The initial research focused on the utilization of photochromic compounds namely spiropyrans, as chelators for heavy metal extraction. Spiropyrans are organic photochromic compounds that have been widely studied. Upon irradiation with UV or visible light, spiropyrans isomerize between the closed and open forms, in which the open form is comparatively more polar. Metal ions can influence this isomerization process by associating with the open form through the electron-rich oxygen atom. In contrast, visible light produces a high concentration of the closed form, and thus hinders metal-binding. Spiropyrans, therefore, show great potential as photo-reversible metal-complexation agents. The spiropyran derivatives were synthesized and immobilized on solid supports including polymeric resins and poly(methylmethacrylate) (PMMA) microchips. Metal ion uptake can be triggered using UV light and subsequently reversed on demand by shining green light on the colored complex, which regenerates the inactive spiropyran form resulting in the release of metal ions. The use of light to trigger the chelator offers unique opportunities. The work continued with the development of microfluidic fabrication for creating versatile, solvent-compatible microfluidic devices through surface modification. There are challenges associated with the use of polymeric based microfluidic devices, particularly in surface modification steps, as solvents can embrittle thermoplastics that resulting in microcracking. To overcome this limitation, we explored the possibility of fabricating extremely robust microfluidic devices entirely from fluorocarbon and glass materials. The work demonstrated a new fabrication technique based on a chemically activated poly(tetrafluoro ethylene) (PTFE) sheet sandwiched between chemically activated glass substrates. The PTFE microchannels can be fabricated in minutes using a cutting plotter to create microchannels. The possibility of glass to PTFE makes this method applicable in a wide range of applications. Advisors/Committee Members: Remcho, Vincent T. (advisor), Walker, Jeffrey R. (committee member).

Subjects/Keywords: Microfluidics; Microfluidic devices

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Nammoonnoy, J. (2010). A photoactivable microfluidic device for heavy metal ion extraction. (Doctoral Dissertation). Oregon State University. Retrieved from http://hdl.handle.net/1957/18461

Chicago Manual of Style (16^th Edition):

Nammoonnoy, Jintana. “A photoactivable microfluidic device for heavy metal ion extraction.” 2010. Doctoral Dissertation, Oregon State University. Accessed March 02, 2021. http://hdl.handle.net/1957/18461.

MLA Handbook (7^th Edition):

Nammoonnoy, Jintana. “A photoactivable microfluidic device for heavy metal ion extraction.” 2010. Web. 02 Mar 2021.

Vancouver:

Nammoonnoy J. A photoactivable microfluidic device for heavy metal ion extraction. [Internet] [Doctoral dissertation]. Oregon State University; 2010. [cited 2021 Mar 02]. Available from: http://hdl.handle.net/1957/18461.

Council of Science Editors:

Nammoonnoy J. A photoactivable microfluidic device for heavy metal ion extraction. [Doctoral Dissertation]. Oregon State University; 2010. Available from: http://hdl.handle.net/1957/18461

Hong Kong University of Science and Technology

12. Tian, Qian CHEM. Fabrication of microfluidic devices and biomaterial design for biological applications.

Degree: 2017, Hong Kong University of Science and Technology

URL: http://repository.ust.hk/ir/Record/1783.1-102102 ; https://doi.org/10.14711/thesis-b1781150 ; http://repository.ust.hk/ir/bitstream/1783.1-102102/1/th_redirect.html

► Microfluidics is an emerging science and technology that manipulates the fluids in channels of tens of micrometers. It provides better solutions in fields of chemistry,… (more)

Subjects/Keywords: Microfluidics ; Microfluidic devices

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APA (6^th Edition):

Tian, Q. C. (2017). Fabrication of microfluidic devices and biomaterial design for biological applications. (Thesis). Hong Kong University of Science and Technology. Retrieved from http://repository.ust.hk/ir/Record/1783.1-102102 ; https://doi.org/10.14711/thesis-b1781150 ; http://repository.ust.hk/ir/bitstream/1783.1-102102/1/th_redirect.html

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Tian, Qian CHEM. “Fabrication of microfluidic devices and biomaterial design for biological applications.” 2017. Thesis, Hong Kong University of Science and Technology. Accessed March 02, 2021. http://repository.ust.hk/ir/Record/1783.1-102102 ; https://doi.org/10.14711/thesis-b1781150 ; http://repository.ust.hk/ir/bitstream/1783.1-102102/1/th_redirect.html.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Tian, Qian CHEM. “Fabrication of microfluidic devices and biomaterial design for biological applications.” 2017. Web. 02 Mar 2021.

Vancouver:

Tian QC. Fabrication of microfluidic devices and biomaterial design for biological applications. [Internet] [Thesis]. Hong Kong University of Science and Technology; 2017. [cited 2021 Mar 02]. Available from: http://repository.ust.hk/ir/Record/1783.1-102102 ; https://doi.org/10.14711/thesis-b1781150 ; http://repository.ust.hk/ir/bitstream/1783.1-102102/1/th_redirect.html.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Tian QC. Fabrication of microfluidic devices and biomaterial design for biological applications. [Thesis]. Hong Kong University of Science and Technology; 2017. Available from: http://repository.ust.hk/ir/Record/1783.1-102102 ; https://doi.org/10.14711/thesis-b1781150 ; http://repository.ust.hk/ir/bitstream/1783.1-102102/1/th_redirect.html

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Hong Kong University of Science and Technology

13. Tian, Jingxuan PHYS. A valve-free 2D concentration gradient generator.

Degree: 2016, Hong Kong University of Science and Technology

URL: http://repository.ust.hk/ir/Record/1783.1-97837 ; https://doi.org/10.14711/thesis-b1610623 ; http://repository.ust.hk/ir/bitstream/1783.1-97837/1/th_redirect.html

► This thesis mainly studies topics in microfluidic chips. Microfluidic chip is a promising platform for multiple discipline, like biology, medicine, chemistry and material synthesis. It… (more)

Subjects/Keywords: Microfluidic devices ; Microfluidics

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APA (6^th Edition):

Tian, J. P. (2016). A valve-free 2D concentration gradient generator. (Thesis). Hong Kong University of Science and Technology. Retrieved from http://repository.ust.hk/ir/Record/1783.1-97837 ; https://doi.org/10.14711/thesis-b1610623 ; http://repository.ust.hk/ir/bitstream/1783.1-97837/1/th_redirect.html

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Tian, Jingxuan PHYS. “A valve-free 2D concentration gradient generator.” 2016. Thesis, Hong Kong University of Science and Technology. Accessed March 02, 2021. http://repository.ust.hk/ir/Record/1783.1-97837 ; https://doi.org/10.14711/thesis-b1610623 ; http://repository.ust.hk/ir/bitstream/1783.1-97837/1/th_redirect.html.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Tian, Jingxuan PHYS. “A valve-free 2D concentration gradient generator.” 2016. Web. 02 Mar 2021.

Vancouver:

Tian JP. A valve-free 2D concentration gradient generator. [Internet] [Thesis]. Hong Kong University of Science and Technology; 2016. [cited 2021 Mar 02]. Available from: http://repository.ust.hk/ir/Record/1783.1-97837 ; https://doi.org/10.14711/thesis-b1610623 ; http://repository.ust.hk/ir/bitstream/1783.1-97837/1/th_redirect.html.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Tian JP. A valve-free 2D concentration gradient generator. [Thesis]. Hong Kong University of Science and Technology; 2016. Available from: http://repository.ust.hk/ir/Record/1783.1-97837 ; https://doi.org/10.14711/thesis-b1610623 ; http://repository.ust.hk/ir/bitstream/1783.1-97837/1/th_redirect.html

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Hong Kong University of Science and Technology

14. Wang, Jing. A new on-chip immunoassay platform combining yeast surface display and microfluidic system for point-of-care application.

Degree: 2011, Hong Kong University of Science and Technology

URL: http://repository.ust.hk/ir/Record/1783.1-7375 ; https://doi.org/10.14711/thesis-b1155745 ; http://repository.ust.hk/ir/bitstream/1783.1-7375/1/th_redirect.html

► Immunoassay, as a technique that utilizes antibody-antigen interactions for the detection of relevant analytes, plays an important role in our daily life. It can be… (more)

▼ Immunoassay, as a technique that utilizes antibody-antigen interactions for the detection of relevant analytes, plays an important role in our daily life. It can be used for the quantification of proteins and small molecules in a number of different fields, such as medical diagnosis, food safety and environmental monitoring. The analysis of antibodies directed against specific epitopes with high specificity and sensitivity in human serum is becoming an increasingly critical task in medical and life sciences as the process provides detection of a serologic biomarker which enables early diagnosis in many diseases: for example, infectious diseases such as HIV and hepatitis B, autoimmune diseases such as systemic lupus erythematosus (SLE), as well as allergies and cancers. Recently, there is an urgent global need for point-of-care immunoassays which can be operated by end users since early stage antibody detection plays a crucial role in disease diagnosis and treatment. In this thesis work, we developed a new on-chip immunoassay platform, which combines the yeast surface display (YSD) technique and a microfluidic system. By directly counting genetically-engineered yeast cells bound with target analytes, the immunoassay was realized on a microchip tailored for point-of-care applications. Taking advantage of YSD, antigenic reporters are easily to be obtained by simply yeast culture, and the tedious steps of antigen purification and wet-chemistry processes to immobilize probe antigen can be eliminated. At the same time, with individual cells readily counted by routine microscopy, fewer numbers of cells can be detected than fluorescence or chromophore-based methods. Thus, this method has the inherent characteristic to be highly sensitive. Furthermore, controlled laminar flow can be applied to preferentially remove nonspecifically bound cells, improving the selectivity of the assay. We have built a prototypical microfluidic immunoassay device incorporating functionalized planar surface and fluidic force discrimination, and demonstrated multiplexed antibody detection using bifunctional engineered yeast cells. The magnetic bead-assisted method, which utilizes protein G coupled magnetic beads to capture antibody-labeled cells, improves the sensitivity of the YSD-based microfluidic immunoassay by 100 times and provides a detection limit of 0.5 ng/ml. Alternatively, we have also developed a functionalized micro pillar array (MPA)-based microfluidic immunoassay, utilizing capillary force as the driven power to replace pumping. The high surface area of the MPA results in a high possibility for cells to contact and attach to the pillars, and provides a detection limit of 5 ng/ml. This method is believed to have excellent compatibility with common biopsy used in general hospitals, as well as high potential to be integrated into a portable system. In order to achieve real point-of-care antibody detection that can be operated by end users, we have developed a non-optical YSD based microfluidic immunoassay using micro Coulter particle…

Subjects/Keywords: Immunoassay ; Microfluidic devices

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APA (6^th Edition):

Wang, J. (2011). A new on-chip immunoassay platform combining yeast surface display and microfluidic system for point-of-care application. (Thesis). Hong Kong University of Science and Technology. Retrieved from http://repository.ust.hk/ir/Record/1783.1-7375 ; https://doi.org/10.14711/thesis-b1155745 ; http://repository.ust.hk/ir/bitstream/1783.1-7375/1/th_redirect.html

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Wang, Jing. “A new on-chip immunoassay platform combining yeast surface display and microfluidic system for point-of-care application.” 2011. Thesis, Hong Kong University of Science and Technology. Accessed March 02, 2021. http://repository.ust.hk/ir/Record/1783.1-7375 ; https://doi.org/10.14711/thesis-b1155745 ; http://repository.ust.hk/ir/bitstream/1783.1-7375/1/th_redirect.html.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Wang, Jing. “A new on-chip immunoassay platform combining yeast surface display and microfluidic system for point-of-care application.” 2011. Web. 02 Mar 2021.

Vancouver:

Wang J. A new on-chip immunoassay platform combining yeast surface display and microfluidic system for point-of-care application. [Internet] [Thesis]. Hong Kong University of Science and Technology; 2011. [cited 2021 Mar 02]. Available from: http://repository.ust.hk/ir/Record/1783.1-7375 ; https://doi.org/10.14711/thesis-b1155745 ; http://repository.ust.hk/ir/bitstream/1783.1-7375/1/th_redirect.html.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Wang J. A new on-chip immunoassay platform combining yeast surface display and microfluidic system for point-of-care application. [Thesis]. Hong Kong University of Science and Technology; 2011. Available from: http://repository.ust.hk/ir/Record/1783.1-7375 ; https://doi.org/10.14711/thesis-b1155745 ; http://repository.ust.hk/ir/bitstream/1783.1-7375/1/th_redirect.html

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Queens University

15. Swyer, Ian. Considerations on the use of Impedance Spectroscopy for the Detection of Virions Trapped in Quadrupolar Microelectrode Arrays .

Degree: Chemical Engineering, 2011, Queens University

URL: http://hdl.handle.net/1974/6528

► The impedance response of a quadrupolar microelectrode array was studied over a wide frequency range to determine whether particles captured at the center of the… (more)

▼ The impedance response of a quadrupolar microelectrode array was studied over a wide frequency range to determine whether particles captured at the center of the array could be detected impedimetrically. The microelectrode array (denoted as DEP chip) uses dielectrophoretic forces to concentrate particles at its center. Initial results showed that there was a large electrode-silicon-electrode (ESE) capacitance which dominated at high frequencies. This capacitance was reduced by decreasing the electrode area and increasing the insulating layer thickness. These measures however proved fruitless as this capacitance was still significantly greater then the dielectric capacitance of the chip. This ESE capacitance can be eliminated through the use of a glass substrate so that the dielectric response of the chip dominates at higher frequencies. Since the ESE capacitance prevented experimental validation of impedance spectroscopy as a signal transduction method, computer simulations were performed. These simulations indicated that capture with the current DEP chips would not have a significant impact on the impedance of the chip. Decreasing the electrode gap distance and reducing the area of the electrodes, which is recommended for future work, can remedy this. As measureable changes in the dielectric capacitance of the chip are not possible, a reaction scheme was developed to translate the capture of viral particles into a change in medium conductivity. An ELISA type system was proposed where the viral particles would be functionalized with urease. This uease would then be used to degrade non-ionic urea into ionic products thereby increasing the medium conductivity. A model was formulated to predict the conductivity increase expected for low concentrations, and validated using higher concentrations of biotinylated-urease. Urease from commercial sources proved not to be a viable option as it does not possess a high enough activity to produce a significant conductivity change given the low concentrations of viral particles expected after collection. Urease with suitable activity is produced by the organism Ureaplasma urealyticum which has an activity of 180 000 µmol urea catalyzed min-1 mg urease-1. It is not recommended that this method be pursued further due to technical challenges that would be encountered.

Subjects/Keywords: Impedance ; Biosensors ; Microfluidic

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APA (6^th Edition):

Swyer, I. (2011). Considerations on the use of Impedance Spectroscopy for the Detection of Virions Trapped in Quadrupolar Microelectrode Arrays . (Thesis). Queens University. Retrieved from http://hdl.handle.net/1974/6528

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Swyer, Ian. “Considerations on the use of Impedance Spectroscopy for the Detection of Virions Trapped in Quadrupolar Microelectrode Arrays .” 2011. Thesis, Queens University. Accessed March 02, 2021. http://hdl.handle.net/1974/6528.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Swyer, Ian. “Considerations on the use of Impedance Spectroscopy for the Detection of Virions Trapped in Quadrupolar Microelectrode Arrays .” 2011. Web. 02 Mar 2021.

Vancouver:

Swyer I. Considerations on the use of Impedance Spectroscopy for the Detection of Virions Trapped in Quadrupolar Microelectrode Arrays . [Internet] [Thesis]. Queens University; 2011. [cited 2021 Mar 02]. Available from: http://hdl.handle.net/1974/6528.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Swyer I. Considerations on the use of Impedance Spectroscopy for the Detection of Virions Trapped in Quadrupolar Microelectrode Arrays . [Thesis]. Queens University; 2011. Available from: http://hdl.handle.net/1974/6528

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Utah

16. Booth, Ross Hunter. A microfluidic in vitro model of the blood-brain barrier.

Degree: PhD, Bioengineering, 2014, University of Utah

URL: http://content.lib.utah.edu/cdm/singleitem/collection/etd3/id/3355/rec/53

► The blood-brain barrier (BBB) limits entry of most molecules into the brain and complicates the development of brain-targeting compounds, necessitating novel BBB models. This dissertation… (more)

▼ The blood-brain barrier (BBB) limits entry of most molecules into the brain and complicates the development of brain-targeting compounds, necessitating novel BBB models. This dissertation describes the first microfluidic BBB model allowing the study of BBB properties in relation to various chemical compounds by enabling tunable wall shear stress (WSS) via dynamic fluid flow, cell-cell interaction through a thin co-culture membrane, time-dependent delivery of test compounds, and integration of sensors into the system, resulting in significant reduction of reagents and cells required and shorter cell seeding time. Use of parallel channels first enabled simultaneous monitoring of multiple cell populations under a wide range (~x15) of WSS.The microfluidic model formed the BBB by incorporating brain endothelial (b.End3) and glial (C6/C8D1A) cells at the intersection of two crossing microchannels, respectively representing luminal and abluminal sides, fabricated in a transparent polydimethylsiloxane (PDMS) substrate utilizing high-precision soft lithography techniques. The utilized cells were adopted from immortalized cells for high consistency over repeated passages and pure and proliferative culture.The developed microfluidic BBB model was validated by (1) expression of tight junction protein ZO-1 and glial protein GFAP by fluorescence imaging, and P-gp activity by Calcein AM, confirming key BBB proteins; (2) high trans-endothelial electrical resistance (TEER) of co-cultures exceeding 250Ωcm2 confirming sufficiently contiguous cell layer formation; (3) chemically-induced barrier modulation, with transient TEER loss by 150μM histamine (~50% for 8-15min), and increase in permeability at elevated pH (10.0); (4) size-dependent (668-70,000Da) compound permeability mimicking in vivo trends; and (5) highly linear correlation (R2>0.85) of clearance rates of seven selected neural drugs with in vivo brain/plasma ratios. We demonstrated the effects of WSS (0-86dyn/cm2) on bEnd.3 properties under increasing WSS, including increase in (6) TEER, (7) cell re-alignment toward flow direction, and (8) protein expression of ZO-1/P-gp, and (9) decrease in tracer permeability.The developed in vitro microfluidic BBB model provides distinct advantages for monitoring and modulating barrier functions and prediction of compound permeability. Thus, it would provide an innovative platform to study mechanisms and pathology of barrier function as well as to assess novel pharmaceuticals early in development for their BBB clearance capabilities.

Subjects/Keywords: Blood-brain barrier; MEMS; Microfluidic

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Booth, R. H. (2014). A microfluidic in vitro model of the blood-brain barrier. (Doctoral Dissertation). University of Utah. Retrieved from http://content.lib.utah.edu/cdm/singleitem/collection/etd3/id/3355/rec/53

Chicago Manual of Style (16^th Edition):

Booth, Ross Hunter. “A microfluidic in vitro model of the blood-brain barrier.” 2014. Doctoral Dissertation, University of Utah. Accessed March 02, 2021. http://content.lib.utah.edu/cdm/singleitem/collection/etd3/id/3355/rec/53.

MLA Handbook (7^th Edition):

Booth, Ross Hunter. “A microfluidic in vitro model of the blood-brain barrier.” 2014. Web. 02 Mar 2021.

Vancouver:

Booth RH. A microfluidic in vitro model of the blood-brain barrier. [Internet] [Doctoral dissertation]. University of Utah; 2014. [cited 2021 Mar 02]. Available from: http://content.lib.utah.edu/cdm/singleitem/collection/etd3/id/3355/rec/53.

Council of Science Editors:

Booth RH. A microfluidic in vitro model of the blood-brain barrier. [Doctoral Dissertation]. University of Utah; 2014. Available from: http://content.lib.utah.edu/cdm/singleitem/collection/etd3/id/3355/rec/53

University of Alberta

17. Olson, Steven. Charge transport in molecular junctions and microfluidic devices.

Degree: MS, Department of Physics, 2010, University of Alberta

URL: https://era.library.ualberta.ca/files/gx41mj807

► Electro-transmittance of molecular junctions was characterized electrically and studied optically at 410nm and 532nm. Between 1kHz and 100kHz there was no qualitative difference between the… (more)

Subjects/Keywords: microfluidic; molecular junctions; charge transport

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APA (6^th Edition):

Olson, S. (2010). Charge transport in molecular junctions and microfluidic devices. (Masters Thesis). University of Alberta. Retrieved from https://era.library.ualberta.ca/files/gx41mj807

Chicago Manual of Style (16^th Edition):

Olson, Steven. “Charge transport in molecular junctions and microfluidic devices.” 2010. Masters Thesis, University of Alberta. Accessed March 02, 2021. https://era.library.ualberta.ca/files/gx41mj807.

MLA Handbook (7^th Edition):

Olson, Steven. “Charge transport in molecular junctions and microfluidic devices.” 2010. Web. 02 Mar 2021.

Vancouver:

Olson S. Charge transport in molecular junctions and microfluidic devices. [Internet] [Masters thesis]. University of Alberta; 2010. [cited 2021 Mar 02]. Available from: https://era.library.ualberta.ca/files/gx41mj807.

Council of Science Editors:

Olson S. Charge transport in molecular junctions and microfluidic devices. [Masters Thesis]. University of Alberta; 2010. Available from: https://era.library.ualberta.ca/files/gx41mj807

University of Alberta

18. Zhang, Le. Functionalized Bead Based Microchip for Immunoassay and Virus Immuno-affinity Chromatography.

Degree: MS, Department of Chemistry, 2014, University of Alberta

URL: https://era.library.ualberta.ca/files/cth83kz515

► We demonstrate the functionalization of a highly ordered porous molecular sieving matrix created by colloidal self-assembly (CSA) of 2 micrometer diameter silica particles in microfluidic… (more)

▼ We demonstrate the functionalization of a highly ordered porous molecular sieving matrix created by colloidal self-assembly (CSA) of 2 micrometer diameter silica particles in microfluidic chips, for highly efficient immuno-capture of viruses. By tuning the particle size, with appropriate surface chemistry, we can easily match the pore size which could maximize biological species-particle wall collision, leading high capture efficiency. The ordered uniform lattice of pores, which are 15 % of the size of the particles used, should prove ideal for the capture of viruses (on the 50-150 nm scale in size). We report on characterization of capture beds with 300 nm pores. These beds were used to detect fluorescein using immobilized anti-fluorescein on the bed, and also to capture and detect type-5 adenovirus in suspension, and in infected cells using appropriate antibodies. We demonstrate the performance of the concept using a fully packed column for fluorescein immunoassay. We used 2 μm carboxylated silica particles self-assembled into a 6 mm long-bed, modified with EDC/NHS, and immobilized anti-fluorescein antibody or type-5 adenovirus-recognizing antibody. An electric field was applied to utilize electro-osmotic flow as the solvent pumping force. A 4.51 nM fluorescein solution was captured by immuno-affinity using anti-fluorescein antibody, and then released with an eluent to an empty downstream analysis region to give a very large signal, providing a positive control. Similar experiments were performed with type-5 adenovirus, demonstrating detection at a concentration of 8.3 × 103 viral particles (VP) per milliliter. Adenovirus in celllysate was also detected in this microchip, with the lowest concentration detectable at 1.5 × 103 PFU/mL. Combination of advanced biological detection methods with microfluidics based extraction and concentration techniques has tremendous potential for realization of a portable, cost effective and sensitive pathogen detection system. Taking advantage of the unique fluid flow characteristics of CSA structures on an even smaller scale than employed here, faster, more sensitive and more economical capture and pre-concentration techniques for diagnostic assays for a wider range of analytes can be developed.

Subjects/Keywords: Microfluidic; Immunoassay; Immuno-affinity Chromatography

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APA (6^th Edition):

Zhang, L. (2014). Functionalized Bead Based Microchip for Immunoassay and Virus Immuno-affinity Chromatography. (Masters Thesis). University of Alberta. Retrieved from https://era.library.ualberta.ca/files/cth83kz515

Chicago Manual of Style (16^th Edition):

Zhang, Le. “Functionalized Bead Based Microchip for Immunoassay and Virus Immuno-affinity Chromatography.” 2014. Masters Thesis, University of Alberta. Accessed March 02, 2021. https://era.library.ualberta.ca/files/cth83kz515.

MLA Handbook (7^th Edition):

Zhang, Le. “Functionalized Bead Based Microchip for Immunoassay and Virus Immuno-affinity Chromatography.” 2014. Web. 02 Mar 2021.

Vancouver:

Zhang L. Functionalized Bead Based Microchip for Immunoassay and Virus Immuno-affinity Chromatography. [Internet] [Masters thesis]. University of Alberta; 2014. [cited 2021 Mar 02]. Available from: https://era.library.ualberta.ca/files/cth83kz515.

Council of Science Editors:

Zhang L. Functionalized Bead Based Microchip for Immunoassay and Virus Immuno-affinity Chromatography. [Masters Thesis]. University of Alberta; 2014. Available from: https://era.library.ualberta.ca/files/cth83kz515

Oregon State University

19. Pluess, Christoph. Application of controlled thermal expansion in diffusion bonding for the high-volume microlamination of MECS devices.

Degree: MS, Industrial Engineering, 2004, Oregon State University

URL: http://hdl.handle.net/1957/30100

Subjects/Keywords: Microfluidic devices

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APA (6^th Edition):

Pluess, C. (2004). Application of controlled thermal expansion in diffusion bonding for the high-volume microlamination of MECS devices. (Masters Thesis). Oregon State University. Retrieved from http://hdl.handle.net/1957/30100

Chicago Manual of Style (16^th Edition):

Pluess, Christoph. “Application of controlled thermal expansion in diffusion bonding for the high-volume microlamination of MECS devices.” 2004. Masters Thesis, Oregon State University. Accessed March 02, 2021. http://hdl.handle.net/1957/30100.

MLA Handbook (7^th Edition):

Pluess, Christoph. “Application of controlled thermal expansion in diffusion bonding for the high-volume microlamination of MECS devices.” 2004. Web. 02 Mar 2021.

Vancouver:

Pluess C. Application of controlled thermal expansion in diffusion bonding for the high-volume microlamination of MECS devices. [Internet] [Masters thesis]. Oregon State University; 2004. [cited 2021 Mar 02]. Available from: http://hdl.handle.net/1957/30100.

Council of Science Editors:

Pluess C. Application of controlled thermal expansion in diffusion bonding for the high-volume microlamination of MECS devices. [Masters Thesis]. Oregon State University; 2004. Available from: http://hdl.handle.net/1957/30100

Cornell University

20. Yu, Yizui. Passive Microfluidic Mixer Fabrication And Flow Test.

Degree: M.S., Applied Physics, Applied Physics, 2012, Cornell University

URL: http://hdl.handle.net/1813/31192

► This study proposed different materials of microfluidic mixer fabrication and their flow test. Two different design based on a T-mixer were fabricated. J-shaped and vertical… (more)

Subjects/Keywords: Microfluidic mixer; fabrication; flow test

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APA (6^th Edition):

Yu, Y. (2012). Passive Microfluidic Mixer Fabrication And Flow Test. (Masters Thesis). Cornell University. Retrieved from http://hdl.handle.net/1813/31192

Chicago Manual of Style (16^th Edition):

Yu, Yizui. “Passive Microfluidic Mixer Fabrication And Flow Test.” 2012. Masters Thesis, Cornell University. Accessed March 02, 2021. http://hdl.handle.net/1813/31192.

MLA Handbook (7^th Edition):

Yu, Yizui. “Passive Microfluidic Mixer Fabrication And Flow Test.” 2012. Web. 02 Mar 2021.

Vancouver:

Yu Y. Passive Microfluidic Mixer Fabrication And Flow Test. [Internet] [Masters thesis]. Cornell University; 2012. [cited 2021 Mar 02]. Available from: http://hdl.handle.net/1813/31192.

Council of Science Editors:

Yu Y. Passive Microfluidic Mixer Fabrication And Flow Test. [Masters Thesis]. Cornell University; 2012. Available from: http://hdl.handle.net/1813/31192

Vanderbilt University

21. Ehrman, Jonathan David. Probing Cell Signaling Networks in Microfluidic Devices.

Degree: PhD, Physics, 2016, Vanderbilt University

URL: http://hdl.handle.net/1803/14608

► Detection of signals is critical for the function of eukaryotic cells. D. discoideum cells are particularly adept at responding chemical gradients, sensing single percent concentration… (more)

Subjects/Keywords: information theory; gut; Microfluidic

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APA (6^th Edition):

Ehrman, J. D. (2016). Probing Cell Signaling Networks in Microfluidic Devices. (Doctoral Dissertation). Vanderbilt University. Retrieved from http://hdl.handle.net/1803/14608

Chicago Manual of Style (16^th Edition):

Ehrman, Jonathan David. “Probing Cell Signaling Networks in Microfluidic Devices.” 2016. Doctoral Dissertation, Vanderbilt University. Accessed March 02, 2021. http://hdl.handle.net/1803/14608.

MLA Handbook (7^th Edition):

Ehrman, Jonathan David. “Probing Cell Signaling Networks in Microfluidic Devices.” 2016. Web. 02 Mar 2021.

Vancouver:

Ehrman JD. Probing Cell Signaling Networks in Microfluidic Devices. [Internet] [Doctoral dissertation]. Vanderbilt University; 2016. [cited 2021 Mar 02]. Available from: http://hdl.handle.net/1803/14608.

Council of Science Editors:

Ehrman JD. Probing Cell Signaling Networks in Microfluidic Devices. [Doctoral Dissertation]. Vanderbilt University; 2016. Available from: http://hdl.handle.net/1803/14608

Texas A&M University

22. Englert, Derek Lynn. Microfluidic Systems for Investigating Bacterial Chemotaxis and Colonization.

Degree: PhD, Chemical Engineering, 2011, Texas A&M University

URL: http://hdl.handle.net/1969.1/ETD-TAMU-2009-12-7295

► The overall goal of this work was to develop and utilize microfluidic models for investigating bacterial chemotaxis and biofilm formation - phenotypes that play key… (more)

▼ The overall goal of this work was to develop and utilize microfluidic models for investigating bacterial chemotaxis and biofilm formation - phenotypes that play key roles in bacterial infections. Classical methods for investigating chemotaxis and biofilm formation have many limitations and drawbacks. These include being unsuitable for investigating the effect of chemorepellents, non-quantitative readouts, and not accounting for interaction between hydrodynamics and biofilm formation. The novel microfluidic model systems for chemotaxis and biofilm formation developed in this study addresses these drawbacks. Chemotaxis model system development was done in three stages. We first developed two static chemotaxis model systems - the two fluorophore chemotaxis agarose plug assay and the mu Plug assay - for rapidly determining the extent of chemotaxis in a qualitative manner. A key feature of these model systems was the incorporation of dead cells and differential labeling with green and red fluorescent proteins for partitioning the effects of movement due to fluid flow from chemotaxis. The static systems were used to rapidly screen a wide range of conditions for use in the flow-based mu Flow chemotaxis model system. The effect of four major variables - cell preparation method, gradient strength, flow rate in the device, and imaging position - that influence the chemotactic response in the mu Flow was characterized using the repellent taxis from Ni² gradients as the model chemoeffector. Using the mu Flow chemotaxis device, we investigated the chemotaxis of Escherichia coli RP437 to different signals that are present in the human gastrointestinal tract and are likely to be mediators of infection through their effect on chemotaxis. Our data show that the bacterial signal indole is a repellent, while the signals autoinducer-2 (AI-2) and isatin are attractants for E. coli RP437. However, cells exposed to a competing gradient of indole and either AI-2 or isatin, attracts E. coli. The ?Flow device was also used to refute a long-standing view on how the repellent Ni2 is sensed in E. coli. Our data show that only the Tar chemoreceptor is needed for sensing Ni² and the nickel binding protein, NikA, and the Ni² transport system proteins, NikB and NikC, are not required for repellent taxis from nickel. A microfluidic biofilm model was also developed in this study and used in conjunction with a mathematical model to investigate biofilm formation and quorum sensing in closed systems (where biofilm growth and hydrodynamics are interdependent). The mathematical model predictions were experimentally validated using Pseudomonas aeruginosa PA14 in a microfluidic biofilm system at various flow rates. Advisors/Committee Members: Jayaraman, Arul (advisor), Pillai, Suresh D. (committee member), Ugaz, Victor M. (committee member), Wood, Thomas K. (committee member).

Subjects/Keywords: chemotaxis; microfluidic; bacteria; nickel taxis

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APA (6^th Edition):

Englert, D. L. (2011). Microfluidic Systems for Investigating Bacterial Chemotaxis and Colonization. (Doctoral Dissertation). Texas A&M University. Retrieved from http://hdl.handle.net/1969.1/ETD-TAMU-2009-12-7295

Chicago Manual of Style (16^th Edition):

Englert, Derek Lynn. “Microfluidic Systems for Investigating Bacterial Chemotaxis and Colonization.” 2011. Doctoral Dissertation, Texas A&M University. Accessed March 02, 2021. http://hdl.handle.net/1969.1/ETD-TAMU-2009-12-7295.

MLA Handbook (7^th Edition):

Englert, Derek Lynn. “Microfluidic Systems for Investigating Bacterial Chemotaxis and Colonization.” 2011. Web. 02 Mar 2021.

Vancouver:

Englert DL. Microfluidic Systems for Investigating Bacterial Chemotaxis and Colonization. [Internet] [Doctoral dissertation]. Texas A&M University; 2011. [cited 2021 Mar 02]. Available from: http://hdl.handle.net/1969.1/ETD-TAMU-2009-12-7295.

Council of Science Editors:

Englert DL. Microfluidic Systems for Investigating Bacterial Chemotaxis and Colonization. [Doctoral Dissertation]. Texas A&M University; 2011. Available from: http://hdl.handle.net/1969.1/ETD-TAMU-2009-12-7295

Penn State University

23. Saksena, Pulkit. Study of Condensed Phase Reactions Between Hypergolic Propellants using Microreactors.

Degree: 2014, Penn State University

URL: https://submit-etda.libraries.psu.edu/catalog/22410

► The testing of hypergolic propellants has been carried out for decades and the use of hypergolic propellants at a systems level is very well understood.… (more)

▼ The testing of hypergolic propellants has been carried out for decades and the use of hypergolic propellants at a systems level is very well understood. Extensive testing has provided the design and construction of rocket engines which have been very reliable. Safety procedures have been developed for the handling and storage for hypergolic propellants, which all too often are highly toxic. But the condensed phase chemistry for these hypergolic propellants is not very well understood. The models in place for ignition and combustion do not handle the coming together of two liquid streams of these hypergols and the two-phase aspects that are expected once the reactions occur. Moreover, it is not clear what makes a fuel and oxidizer pair hypergolic in nature. The coupling of physical and chemical factors that control the ignition of hypergolic propellants makes the direct study of the transient ignition process difficult. The current study presents a novel method to study hypergolic propellants (very fast liquid reactions) using microreactors instead of conventional drop tests, modified versions thereof or impinging jet tests. Planar counterflow microreactors are used to isolate liquid-phase reactions from secondary gas phase reactions that occur later during the ignition process and thus provide valuable insight in to the pre-ignition mechanism. The microreactor fabrication, flow field characterization, reactivity results from experiments performed using a variety of fuels and nitric acid as the oxidizer and numerical simulation results of reactive flow in the microreactor are presented in this study. Particle image velocimetry measurements and numerical simulations were conducted to characterize the laminar velocity flow-field in the microreactor and strain rates at the stagnation point along the centerline of the microreactor. Temperature measurements at the stagnation zone, the exit and positions along the length of the microreactor were used as a measure for the extent of the reaction or the heat released from the reaction. For the hypergols, an increase in reactant flow (or equivalently strain rate at the stagnation point) was found to initially increase temperatures at both the stagnation zone as well as along the length of the reactor, but eventually resulted in a decrease in temperature, revealing a maxima in temperature and reactivity. The trends indicated a reaction that was initially diffusion or heat loss controlled, which transitioned towards kinetic control at higher strain (flow) rates. Numerical simulations of the reactive flow in the microreactor stagnation zone were also carried out in which the reaction zone in the microreactor was treated similar to the reaction zone occurring in a counter-flow diffusion flame. These simulations showed that the flow rates at which the peak in temperature occurred was dependent on the rate of the reaction occurring between the hypergol pairs. The numerical simulations also show that heat loss from the top and bottom surfaces can play a role on the temperature… Advisors/Committee Members: Richard A Yetter, Dissertation Advisor/Co-Advisor, Srinivas A Tadigadapa, Dissertation Advisor/Co-Advisor, Stefan Thynell, Committee Member, Donghai Wang, Committee Member, Siyang Zheng, Committee Member.

Subjects/Keywords: Hypergolic; Propellants; Combustion; Microfluidic; Microreactor

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APA (6^th Edition):

Saksena, P. (2014). Study of Condensed Phase Reactions Between Hypergolic Propellants using Microreactors. (Thesis). Penn State University. Retrieved from https://submit-etda.libraries.psu.edu/catalog/22410

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Saksena, Pulkit. “Study of Condensed Phase Reactions Between Hypergolic Propellants using Microreactors.” 2014. Thesis, Penn State University. Accessed March 02, 2021. https://submit-etda.libraries.psu.edu/catalog/22410.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Saksena, Pulkit. “Study of Condensed Phase Reactions Between Hypergolic Propellants using Microreactors.” 2014. Web. 02 Mar 2021.

Vancouver:

Saksena P. Study of Condensed Phase Reactions Between Hypergolic Propellants using Microreactors. [Internet] [Thesis]. Penn State University; 2014. [cited 2021 Mar 02]. Available from: https://submit-etda.libraries.psu.edu/catalog/22410.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Saksena P. Study of Condensed Phase Reactions Between Hypergolic Propellants using Microreactors. [Thesis]. Penn State University; 2014. Available from: https://submit-etda.libraries.psu.edu/catalog/22410

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Penn State University

24. Poudyal, Ashutosh. A Study on the Effect of Media Volume Manipulations on Global HUVEC Colony Proliferation Within a Microfluidic Channel.

Degree: 2014, Penn State University

URL: https://submit-etda.libraries.psu.edu/catalog/21829

► Microfluidic-based cell culture is a rapidly developing subject used in studying behavior and external influences on cells on a micro-scale. A majority of modern techniques… (more)

Subjects/Keywords: HUVEC; microfluidic; cell culture

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APA (6^th Edition):

Poudyal, A. (2014). A Study on the Effect of Media Volume Manipulations on Global HUVEC Colony Proliferation Within a Microfluidic Channel. (Thesis). Penn State University. Retrieved from https://submit-etda.libraries.psu.edu/catalog/21829

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Poudyal, Ashutosh. “A Study on the Effect of Media Volume Manipulations on Global HUVEC Colony Proliferation Within a Microfluidic Channel.” 2014. Thesis, Penn State University. Accessed March 02, 2021. https://submit-etda.libraries.psu.edu/catalog/21829.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Poudyal, Ashutosh. “A Study on the Effect of Media Volume Manipulations on Global HUVEC Colony Proliferation Within a Microfluidic Channel.” 2014. Web. 02 Mar 2021.

Vancouver:

Poudyal A. A Study on the Effect of Media Volume Manipulations on Global HUVEC Colony Proliferation Within a Microfluidic Channel. [Internet] [Thesis]. Penn State University; 2014. [cited 2021 Mar 02]. Available from: https://submit-etda.libraries.psu.edu/catalog/21829.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Poudyal A. A Study on the Effect of Media Volume Manipulations on Global HUVEC Colony Proliferation Within a Microfluidic Channel. [Thesis]. Penn State University; 2014. Available from: https://submit-etda.libraries.psu.edu/catalog/21829

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Toronto

25. Tan, Qingyuan. Development of a Microfluidic Device for Single Cell Specific Membrane Capacitance Quantification.

Degree: 2012, University of Toronto

URL: http://hdl.handle.net/1807/33555

►

The specific membrane capacitance (SMC) of biological cell membranes correlates with cells’ electrical activity and morphology, which are physiological markers for cellular phenotype and health.… (more)

Subjects/Keywords: Microfluidic; Cancer cell; 0541

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APA (6^th Edition):

Tan, Q. (2012). Development of a Microfluidic Device for Single Cell Specific Membrane Capacitance Quantification. (Masters Thesis). University of Toronto. Retrieved from http://hdl.handle.net/1807/33555

Chicago Manual of Style (16^th Edition):

Tan, Qingyuan. “Development of a Microfluidic Device for Single Cell Specific Membrane Capacitance Quantification.” 2012. Masters Thesis, University of Toronto. Accessed March 02, 2021. http://hdl.handle.net/1807/33555.

MLA Handbook (7^th Edition):

Tan, Qingyuan. “Development of a Microfluidic Device for Single Cell Specific Membrane Capacitance Quantification.” 2012. Web. 02 Mar 2021.

Vancouver:

Tan Q. Development of a Microfluidic Device for Single Cell Specific Membrane Capacitance Quantification. [Internet] [Masters thesis]. University of Toronto; 2012. [cited 2021 Mar 02]. Available from: http://hdl.handle.net/1807/33555.

Council of Science Editors:

Tan Q. Development of a Microfluidic Device for Single Cell Specific Membrane Capacitance Quantification. [Masters Thesis]. University of Toronto; 2012. Available from: http://hdl.handle.net/1807/33555

Victoria University of Wellington

26. Dhondi, Srikanth. Numerical Simulations of Polymers at the Nanoscale.

Degree: 2011, Victoria University of Wellington

URL: http://hdl.handle.net/10063/1753

► In this thesis we study a variety of nanoscale phenomena in certain polymer systems using a combination of numerical simulation methods and mathematical modelling. The… (more)

▼ In this thesis we study a variety of nanoscale phenomena in certain polymer systems using a combination of numerical simulation methods and mathematical modelling. The problems considered are: (a) the mixing behaviour of polymeric fluids in micro- and nanofluidic devices, (b) capillary absorption of polymer droplets into narrow capillaries, and (c) modelling the phase separation and self-assembly behaviour in polymer systems with freely deforming boundaries. These problems are significant in nanotechnological applications of polymer-based systems. First, the mixing behaviour of a polymeric melt over two parallely patternedslip surfaces is considered. Using molecular dynamics (MD) simulations, it is shown that mixing is enhanced when the polymer chain size is smaller than the wavelength of the chemical pattern of the surfaces. An off-set in the upper and lowerwall patterns improved themixing in the centre of the channel. Application of a sinusoidally varying body force in addition to the patterned-slip conditions is shown to enhance mixing further, compared to a constant body force case, with some limitations. Simulation findings for the constant body force cases are in qualitative agreement with the continuum theory of Pereira [1]. However, in the case of a sinusoidally varying body force our simulations do not agree with the continuum theory. We explain the reasons for the discrepancy between the two and point out the deficiencies in the continuum theory in predicting the correct behaviour. Second, the capillary phenomena of polymer droplets in narrow capillaries is studied using MD simulations. It is demonstrated that droplets composed of longer chains require wider tubes for absorption and this result is in agreement with our continuum modelling. The observed capillary dynamics deviate significantly from the standard Lucas-Washburn description thus questioning its validity at the nanoscale. The metastable states during the capillary absorption in some cases cannot be explained using the existing models of capillary dynamics. Lastly, the phase separation process in polymer blends between both confined and unconfined boundaries is studied using Smoothed Particle Hydrodynamics (SPH). The SPH technique has the advantage of not using a grid to discretize the spatial domain, which makes it appealing when dealing with problems where the spatial domain can change with time. The applicability of the SPH method in describing phase separation in these systems is demonstrated. In particular, its ability to model freely deforming polymer blends is shown. Advisors/Committee Members: Hendy, Shaun.

Subjects/Keywords: Molecular dynamics; Microfluidic mixing

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APA (6^th Edition):

Dhondi, S. (2011). Numerical Simulations of Polymers at the Nanoscale. (Doctoral Dissertation). Victoria University of Wellington. Retrieved from http://hdl.handle.net/10063/1753

Chicago Manual of Style (16^th Edition):

Dhondi, Srikanth. “Numerical Simulations of Polymers at the Nanoscale.” 2011. Doctoral Dissertation, Victoria University of Wellington. Accessed March 02, 2021. http://hdl.handle.net/10063/1753.

MLA Handbook (7^th Edition):

Dhondi, Srikanth. “Numerical Simulations of Polymers at the Nanoscale.” 2011. Web. 02 Mar 2021.

Vancouver:

Dhondi S. Numerical Simulations of Polymers at the Nanoscale. [Internet] [Doctoral dissertation]. Victoria University of Wellington; 2011. [cited 2021 Mar 02]. Available from: http://hdl.handle.net/10063/1753.

Council of Science Editors:

Dhondi S. Numerical Simulations of Polymers at the Nanoscale. [Doctoral Dissertation]. Victoria University of Wellington; 2011. Available from: http://hdl.handle.net/10063/1753

Oregon State University

27. Siripoorikan, Bunchong. Flow induced mixing in high aspect ratio microchannels.

Degree: MS, Mechanical Engineering, 2003, Oregon State University

URL: http://hdl.handle.net/1957/31550

► Micro-fluid mixing is an important aspect of many of the various micro-fluidic systems used in biochemical production, biomedical industries, micro-energy systems and some electronic devices.… (more)

▼ Micro-fluid mixing is an important aspect of many of the various micro-fluidic systems used in biochemical production, biomedical industries, micro-energy systems and some electronic devices. Typically, because of size constraints and laminar flow conditions, different fluids may only have the opportunity to mix by diffusion, which is extremely rate limited. Therefore, active or highly effective passive mixing techniques are often required. In this study, two pulsed injectors are used to actively enhance mixing in a high aspect ratio microchannel (125 μm deep and 1 mm wide). The main channel has two adjacent flowing streams with 100% dye and 0% dye concentrations, respectively. Two injectors (125 μm deep and 250 μm wide) are located on separate sides of the channel, with one downstream 2 mm (equivalent to two main channel widths or eight injector widths) from the other. This results in an asymmetric mixing as the flow proceeds downstream. A dye solution is used to map local mixing throughout the channel by measuring concentration variations as a function of both space and time. The primary flow rates are varied from 0.01 to 0.20 ml/min (Reynolds numbers of 0.3 to 26.6), the injector flow rate ratios are varied from 0.125 to 2, and the pulsing frequencies are varied from 5 to 15 Hz. Images of the concentration variations within the channel are used to quantify mixing by calibrating the intensity of the image with the concentration of the dye solution. The degree of mixing (DoM) is used as a measure of quality and is defined based on the integration across the channel of the difference between the local concentration and the 50% concentration values. DoM is normalized by the 50% concentration value and subtracted from one to yield a parameter that varies from 0 (no mixing) to 1 (perfect mixing). It is shown that there is a high degree of repeatability of concentration distribution as a function of phase of the pulsing cycle. A mixing map is constructed over the range of variables tested which indicates an optimum set of flow and pulsing conditions needed to achieve maximum mixing in the main channel flow. The flow rate ratio between the injectors and main channel is found to be the most influential parameter on overall mixing. The highest DoM in the main channel was found to be 0.89. It is also noticed that improved mixing can occur at very low flow ratios under a unique set of primary flow and low frequency pulsing conditions. In general, there is an inverse relationship between primary flow rate and pulsing frequency to achieve better overall mixing. Advisors/Committee Members: Liburdy, James A. (advisor).

Subjects/Keywords: Microfluidic devices

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APA (6^th Edition):

Siripoorikan, B. (2003). Flow induced mixing in high aspect ratio microchannels. (Masters Thesis). Oregon State University. Retrieved from http://hdl.handle.net/1957/31550

Chicago Manual of Style (16^th Edition):

Siripoorikan, Bunchong. “Flow induced mixing in high aspect ratio microchannels.” 2003. Masters Thesis, Oregon State University. Accessed March 02, 2021. http://hdl.handle.net/1957/31550.

MLA Handbook (7^th Edition):

Siripoorikan, Bunchong. “Flow induced mixing in high aspect ratio microchannels.” 2003. Web. 02 Mar 2021.

Vancouver:

Siripoorikan B. Flow induced mixing in high aspect ratio microchannels. [Internet] [Masters thesis]. Oregon State University; 2003. [cited 2021 Mar 02]. Available from: http://hdl.handle.net/1957/31550.

Council of Science Editors:

Siripoorikan B. Flow induced mixing in high aspect ratio microchannels. [Masters Thesis]. Oregon State University; 2003. Available from: http://hdl.handle.net/1957/31550

Oregon State University

28. Enfield, Kent E. Laminate mixing in microscale fractal-like merging channel networks.

Degree: MS, Mechanical Engineering, 2003, Oregon State University

URL: http://hdl.handle.net/1957/32377

► A two-dimensional model was developed to predict concentration profiles from passive, laminar mixing of concentration layers formed in a fractal-like merging channel network. Both flat… (more)

Subjects/Keywords: Microfluidic devices

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APA (6^th Edition):

Enfield, K. E. (2003). Laminate mixing in microscale fractal-like merging channel networks. (Masters Thesis). Oregon State University. Retrieved from http://hdl.handle.net/1957/32377

Chicago Manual of Style (16^th Edition):

Enfield, Kent E. “Laminate mixing in microscale fractal-like merging channel networks.” 2003. Masters Thesis, Oregon State University. Accessed March 02, 2021. http://hdl.handle.net/1957/32377.

MLA Handbook (7^th Edition):

Enfield, Kent E. “Laminate mixing in microscale fractal-like merging channel networks.” 2003. Web. 02 Mar 2021.

Vancouver:

Enfield KE. Laminate mixing in microscale fractal-like merging channel networks. [Internet] [Masters thesis]. Oregon State University; 2003. [cited 2021 Mar 02]. Available from: http://hdl.handle.net/1957/32377.

Council of Science Editors:

Enfield KE. Laminate mixing in microscale fractal-like merging channel networks. [Masters Thesis]. Oregon State University; 2003. Available from: http://hdl.handle.net/1957/32377

University of Adelaide

29. Zhang, Mengjue. Microfluidic system development for drug delivery.

Degree: 2016, University of Adelaide

URL: http://hdl.handle.net/2440/102614

► Development and application of a microfluidic system for generating drug delivery carriers are investigated in this research. Various types of microfluidic devices are designed and… (more)

▼ Development and application of a microfluidic system for generating drug delivery carriers are investigated in this research. Various types of microfluidic devices are designed and fabricated for peptide nanotubes, liposome vesicles and double emulsions formation. The microfluidic system offers a better control over the formation process of all three drug delivery carriers. Comparing to traditional methods such as bulk mixing, the process efficiency, size and size distribution of the final products are significantly improved. The results generated show that tuning the flow rate ratios between different reagents from the inlet streams successfully controls the sizes and size distributions of liposomes vesicles. The relationship between the flow rate ratio and the size of the resulting vesicles is established. Macrocycle (AP-169) that was found to self-assemble into an anti-parallel β-sheet nanotube with a triggering agent is successfully synthesized and purified for peptide nanotube self-assembling process. A microfluidic device is designed and fabricated to control the interaction between AP-169 and its self-assembling triggering agent, dimethyl sulfoxide. Double emulsions with different radii are produced with the microfluidic system by adjusting the flow rate ratio between each phase of the solution, and changing the wetting properties of the microchannels. The stability of double emulsions is enhanced by introducing various surfactants. The sizes and size distributions of liposomes and double emulsions have been successfully controlled and optimized for drug delivery. In conclusion, various drug delivery carriers have been successfully generated and optimized with a designed and modified microfluidic system. These products can be further applied in drug encapsulation, biomolecular screening and in vitro compartmentalization in the future. Advisors/Committee Members: Bi, Jingxiu (advisor), Zhang, Hu (advisor), Biggs, Mark James (advisor), School of Chemical Engineering (school).

Subjects/Keywords: microfluidic; liposomes; double emulsions

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Zhang, M. (2016). Microfluidic system development for drug delivery. (Thesis). University of Adelaide. Retrieved from http://hdl.handle.net/2440/102614

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Zhang, Mengjue. “Microfluidic system development for drug delivery.” 2016. Thesis, University of Adelaide. Accessed March 02, 2021. http://hdl.handle.net/2440/102614.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Zhang, Mengjue. “Microfluidic system development for drug delivery.” 2016. Web. 02 Mar 2021.

Vancouver:

Zhang M. Microfluidic system development for drug delivery. [Internet] [Thesis]. University of Adelaide; 2016. [cited 2021 Mar 02]. Available from: http://hdl.handle.net/2440/102614.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Zhang M. Microfluidic system development for drug delivery. [Thesis]. University of Adelaide; 2016. Available from: http://hdl.handle.net/2440/102614

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Toronto

30. Crocker, Alana. Examining the Effect of Laminar Flow on Ex Vivo Pancreatic Islet Associated Endothelial Cells.

Degree: 2010, University of Toronto

URL: http://hdl.handle.net/1807/25460

►

Pancreatic islets are heavily vascularized micro-organs containing insulin secreting beta-cells coupled with endothelial cells (EC). These EC slowly deteriorate in static culture, precluding long term… (more)

Subjects/Keywords: Endothelial Cells; Microfluidic; 0541

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Crocker, A. (2010). Examining the Effect of Laminar Flow on Ex Vivo Pancreatic Islet Associated Endothelial Cells. (Masters Thesis). University of Toronto. Retrieved from http://hdl.handle.net/1807/25460

Chicago Manual of Style (16^th Edition):

Crocker, Alana. “Examining the Effect of Laminar Flow on Ex Vivo Pancreatic Islet Associated Endothelial Cells.” 2010. Masters Thesis, University of Toronto. Accessed March 02, 2021. http://hdl.handle.net/1807/25460.

MLA Handbook (7^th Edition):

Crocker, Alana. “Examining the Effect of Laminar Flow on Ex Vivo Pancreatic Islet Associated Endothelial Cells.” 2010. Web. 02 Mar 2021.

Vancouver:

Crocker A. Examining the Effect of Laminar Flow on Ex Vivo Pancreatic Islet Associated Endothelial Cells. [Internet] [Masters thesis]. University of Toronto; 2010. [cited 2021 Mar 02]. Available from: http://hdl.handle.net/1807/25460.

Council of Science Editors:

Crocker A. Examining the Effect of Laminar Flow on Ex Vivo Pancreatic Islet Associated Endothelial Cells. [Masters Thesis]. University of Toronto; 2010. Available from: http://hdl.handle.net/1807/25460

Open Access Theses and Dissertations