subject:(Microfluidic)

Oregon State University

1. Weymann, Davis. Properties of hydrogels for immobilization of bacteria in a commercial microfluidic dioreactor.

Degree: MS, 2017, Oregon State University

URL: http://hdl.handle.net/1957/61554

► Current technological shortcomings limit the economic viability of capturing and utilizing small sources of methane. The development of a reactor to overcome these limitations would… (more)

▼ Current technological shortcomings limit the economic viability of capturing and utilizing small sources of methane. The development of a reactor to overcome these limitations would unlock economic opportunity and incentivize reduced methane emissions. A microfluidic bioreactor containing immobilized methanotrophs has the potential to overcome these limitations by profitably converting small quantities of methane to more valuable liquid products. The material used for immobilizing bacteria in a microfluidic bioreactor must meet four criteria: biocompatibility, mechanical stability, reactor adhesion, and economic viability. This paper describes the development of a novel blend of agar and cross-linked polyvinyl alcohol (PVA) that meets these requirements. The properties of agar/PVA blend hydrogels strongly depend on the ratio and absolute concentration of the constituent polymers, and the processes by which the polymers are cross-linked. The microscopic morphology of these blend hydrogels is theorized to be two interacting and competing phases formed by agar and PVA molecules mutually interfering with cross-linking via hydrogen bonding, and separating due to spinodal decomposition. Evidence for the proposed morphology is discussed. Blend hydrogels of 2/5% agar/PVA are particularly promising, combining the desirable properties of both agar (low swelling) and PVA (strength and adhesion). The 2/5% agar/PVA gels exhibited little swelling in water and nitrate mineral salts-based media. They also adhered to polycarbonate and stainless steel surfaces treated with ozone or oxygen plasma. Cultures of Methylomicrobium buryatense 5GB1 (5GB1) immobilized in these gels showed a reduction of metabolic activity rates, partly due to exposure to high concentrations of sulfate and phosphate during cross-linking. Shortening cross-linker exposure time from 2 hours to 30 minutes greatly improved activity rates, and immobilized cells exhibited increased activity rates over time as fresh methane was added. Based on these results, the 2/5% agar/PVA blend hydrogels are suitable for the immobilization of 5GB1 in a microfluidic bioreactor. Further improvement of activity rates may be possible. Preservation of 5GB1 by lyophilization was unsuccessful. Cultures preserved in solutions of 5% bovine serum albumin and 10% sucrose or trehalose maintained metabolic activity rates after freezing at -80°C, but showed no activity after lyophilization. Advisors/Committee Members: Schilke, Karl (advisor), Jovanovic, Goran (committee member).

Subjects/Keywords: Microfluidic

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APA (6^th Edition):

Weymann, D. (2017). Properties of hydrogels for immobilization of bacteria in a commercial microfluidic dioreactor. (Masters Thesis). Oregon State University. Retrieved from http://hdl.handle.net/1957/61554

Chicago Manual of Style (16^th Edition):

Weymann, Davis. “Properties of hydrogels for immobilization of bacteria in a commercial microfluidic dioreactor.” 2017. Masters Thesis, Oregon State University. Accessed February 25, 2020. http://hdl.handle.net/1957/61554.

MLA Handbook (7^th Edition):

Weymann, Davis. “Properties of hydrogels for immobilization of bacteria in a commercial microfluidic dioreactor.” 2017. Web. 25 Feb 2020.

Vancouver:

Weymann D. Properties of hydrogels for immobilization of bacteria in a commercial microfluidic dioreactor. [Internet] [Masters thesis]. Oregon State University; 2017. [cited 2020 Feb 25]. Available from: http://hdl.handle.net/1957/61554.

Council of Science Editors:

Weymann D. Properties of hydrogels for immobilization of bacteria in a commercial microfluidic dioreactor. [Masters Thesis]. Oregon State University; 2017. Available from: http://hdl.handle.net/1957/61554

University of Hong Kong

2. Kong, Tiantian. Microfluidic fabrication of polymer-based microparticles for biomedical applications.

Degree: PhD, 2013, University of Hong Kong

URL: Kong, T. [孔湉湉]. (2013). Microfluidic fabrication of polymer-based microparticles for biomedical applications. (Thesis). University of Hong Kong, Pokfulam, Hong Kong SAR. Retrieved from http://dx.doi.org/10.5353/th_b5153689 ; http://dx.doi.org/10.5353/th_b5153689 ; http://hdl.handle.net/10722/196008

► Delivery vehicles that can encapsulate and release active ingredients of pre-determined volumes at the target site on-demand present a challenge in biomedical field. Due to… (more)

▼ Delivery vehicles that can encapsulate and release active ingredients of pre-determined volumes at the target site on-demand present a challenge in biomedical field. Due to their tunable physiochemical properties and degradation rate, polymeric particles are one of the most extensively employed delivery vehicles. Generally they are fabricated from emulsion templates. Conventional bulk emulsification technique provides little control over the characteristics of droplets generated. Thus the properties of the subsequent particles cannot be controlled. The advance of droplet microfluidics enables the generation and manipulation of designer single, double or higher-order emulsion droplets with customizable structure. These droplets are powerful and versatile templates for fabricating polymeric delivery vehicles with pre-determined properties. Due to the monodispersity of droplet templates by microfluidics, the relationship between size, size distribution, shape, architecture, elastic responses and release kinetics can be systematically studied. These understandings are of key importance for the design and fabrication of the next generation polymeric delivery vehicles with custom-made functions for specific applications. In the present work, we engineer the droplet templates generated from microfluidics to fabricate designer polymeric microparticles as delivery vehicles. We investigate and obtain the relationship between the particle size, size distribution, structure of microparticles and their release kinetics. Moreover, we also identify an innovative route to tune the particle shape that enables the investigation of the relationship between particle shape and release kinetics. We take advantage of the dewetting phenomena driving by interfacial tensions of different liquid phases to vary the droplet shape. We find that the phase-separation-induced shape variation of polymeric composite particles can be engineered by manipulating the kinetic barriers during droplet shape evolution. To predict the performance of our advanced polymer particles in practical applications, for instance, in narrow blood vessels in vivo, we also develop a novel capillary micromechanics technique to characterize the linear and non-linear elastic response of our polymer particles on single particle level. The knowledge of the mechanical properties enables the prediction as well as the design of the mechanical aspects of polymer particles in different applications. The ability to control and design the physical, chemical, mechanical properties of the delivery vehicles, and the understanding between these properties and the biological functionalities of delivery vehicles, such as the release kinetics, lead towards tailor-designed delivery vehicles with finely-designed functionalities for various biomedical applications. Our proposed electro-microfluidic platform potentially enables generation of submicron droplet templates with a narrow size distribution and nanoscaled delivery vehicles with well-controlled properties, leading to a next… Advisors/Committee Members: Shum, HC, Wang, L.

Subjects/Keywords: Microfluidic devices

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APA (6^th Edition):

Kong, T. (2013). Microfluidic fabrication of polymer-based microparticles for biomedical applications. (Doctoral Dissertation). University of Hong Kong. Retrieved from Kong, T. [孔湉湉]. (2013). Microfluidic fabrication of polymer-based microparticles for biomedical applications. (Thesis). University of Hong Kong, Pokfulam, Hong Kong SAR. Retrieved from http://dx.doi.org/10.5353/th_b5153689 ; http://dx.doi.org/10.5353/th_b5153689 ; http://hdl.handle.net/10722/196008

Chicago Manual of Style (16^th Edition):

Kong, Tiantian. “Microfluidic fabrication of polymer-based microparticles for biomedical applications.” 2013. Doctoral Dissertation, University of Hong Kong. Accessed February 25, 2020. Kong, T. [孔湉湉]. (2013). Microfluidic fabrication of polymer-based microparticles for biomedical applications. (Thesis). University of Hong Kong, Pokfulam, Hong Kong SAR. Retrieved from http://dx.doi.org/10.5353/th_b5153689 ; http://dx.doi.org/10.5353/th_b5153689 ; http://hdl.handle.net/10722/196008.

MLA Handbook (7^th Edition):

Kong, Tiantian. “Microfluidic fabrication of polymer-based microparticles for biomedical applications.” 2013. Web. 25 Feb 2020.

Vancouver:

Kong T. Microfluidic fabrication of polymer-based microparticles for biomedical applications. [Internet] [Doctoral dissertation]. University of Hong Kong; 2013. [cited 2020 Feb 25]. Available from: Kong, T. [孔湉湉]. (2013). Microfluidic fabrication of polymer-based microparticles for biomedical applications. (Thesis). University of Hong Kong, Pokfulam, Hong Kong SAR. Retrieved from http://dx.doi.org/10.5353/th_b5153689 ; http://dx.doi.org/10.5353/th_b5153689 ; http://hdl.handle.net/10722/196008.

Council of Science Editors:

Kong T. Microfluidic fabrication of polymer-based microparticles for biomedical applications. [Doctoral Dissertation]. University of Hong Kong; 2013. Available from: Kong, T. [孔湉湉]. (2013). Microfluidic fabrication of polymer-based microparticles for biomedical applications. (Thesis). University of Hong Kong, Pokfulam, Hong Kong SAR. Retrieved from http://dx.doi.org/10.5353/th_b5153689 ; http://dx.doi.org/10.5353/th_b5153689 ; http://hdl.handle.net/10722/196008

3. Thelwell, Jeray Anthony. Carrier bead systems in the microfluidic isolation of cfDNA for downstream analysis.

Degree: Biomedical Engineering, 2017, Brown University

URL: https://repository.library.brown.edu/studio/item/bdr:733538/

► The field of medical diagnostics is governed by two practical goals that drive the engineering and relevant innovation necessary to move the technology forward. Firstly,… (more)

▼ The field of medical diagnostics is governed by two practical goals that drive the engineering and relevant innovation necessary to move the technology forward. Firstly, diagnostic technology must have predictive potential. As medical education continues to develop with the growing body of basic biological research, there is increased emphasis on preventative medicine. Instead of training more highly skilled surgical specialty doctors to mechanically remedy exacerbated ailments of the body, lives are now being saved before patients even make it into the operating room. Predictive diagnostic technologies provide a wealth of information, not only about current pathological status, but also about the real-time effects of treatment with little to no harm of the patient. Secondly, diagnostic technology must offer personalized patient information. With completion of the human genome project and the advent of more sophisticated sequencing modalities, medical care tailored to specific individuals is more of a possibility. A greater understanding of epigenetic modulation of the genome is promoting the transition from “one size fits all” care. While approaching this problem in the Tripathi Lab, two additional constraints were imposed: the diagnostic technology must be robust yet economical and simple to use in order to allow for widespread adoption. To that end, a microfluidic platform for the extraction of short fragment DNA from crowded solution is reported with the idea of mimicking cell-free DNA found in blood. Preliminary off-chip experiments comparing different magnetic beads to determine the optimal bead and buffer composition for short DNA fragments are first completed. Then an investigation into multi-bead systems for size based extraction through the oil-water interface is carried out. Finally, on-chip short fragment DNA recovery is demonstrated from buffer solution and spiked human serum solution. Advisors/Committee Members: Tripathi, Anubhav (Advisor), Breuer, Kenneth (Reader), Mathiowitz, Edith (Reader), Wessel, Gary (Reader).

Subjects/Keywords: Microfluidic devices

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APA (6^th Edition):

Thelwell, J. A. (2017). Carrier bead systems in the microfluidic isolation of cfDNA for downstream analysis. (Thesis). Brown University. Retrieved from https://repository.library.brown.edu/studio/item/bdr:733538/

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Thelwell, Jeray Anthony. “Carrier bead systems in the microfluidic isolation of cfDNA for downstream analysis.” 2017. Thesis, Brown University. Accessed February 25, 2020. https://repository.library.brown.edu/studio/item/bdr:733538/.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Thelwell, Jeray Anthony. “Carrier bead systems in the microfluidic isolation of cfDNA for downstream analysis.” 2017. Web. 25 Feb 2020.

Vancouver:

Thelwell JA. Carrier bead systems in the microfluidic isolation of cfDNA for downstream analysis. [Internet] [Thesis]. Brown University; 2017. [cited 2020 Feb 25]. Available from: https://repository.library.brown.edu/studio/item/bdr:733538/.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Thelwell JA. Carrier bead systems in the microfluidic isolation of cfDNA for downstream analysis. [Thesis]. Brown University; 2017. Available from: https://repository.library.brown.edu/studio/item/bdr:733538/

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Oregon State University

4. Rundel, Jack T. Design, fabrication and application of a microfluidic nanofiltration module for separation and purification of macromolecules and nanoparticles.

Degree: PhD, Chemistry, 2008, Oregon State University

URL: http://hdl.handle.net/1957/7682

► A microfluidic nanofiltration module has been designed, fabricated and applied to the continuous-flow, pressure-driven, post-synthetic purification of macromolecules and nanoparticles via diafiltration using a commercially… (more)

▼ A microfluidic nanofiltration module has been designed, fabricated and applied to the continuous-flow, pressure-driven, post-synthetic purification of macromolecules and nanoparticles via diafiltration using a commercially available organic solvent resistant nanofiltration membrane, STARMEM 122. This module will readily interface with other microscale components within a "nanofactory" for the rapid synthesis, purification and delivery of highly dispersed macromolecules and nanoparticles. The microfluidic nanofiltration module (MNM) fully integrates the membrane into an all-polymer format constructed of materials that are relatively inexpensive, chemically compatible with the targeted compounds and transmissive to visible light allowing optical monitoring of the system. A molecular weight cutoff of 2.3 kDa was determined for the MNM using half-generation poly(amidoamine) dendrimer standards in a simulated postsynthetic mixture of 4 mM methyl acrylate in methanol. The membrane was also characterized within a macroscale test fixture (MTF), constructed of reusable parts that are easily disassembled for rapid replacement of the membrane. Rejections of 95% to 97% were observed in the MTF for triphenylphosphine (PPh3) stabilized gold-eleven (Au11) nanoparticle standards. Rejections of 12% to 58% were also observed for PPh₃, an anticipated post-synthetic byproduct. Purification via diafiltration of realworld post-synthetic Au₁₁ mixtures was demonstrated in the MTF. Au₁₁ rejections > 99% were observed along with expected decreases in PPh₃ concentrations. Stable permeances of 2 L m⁻² h⁻¹ bar⁻¹ were also observed, comparable to those reported in the literature and claimed by the membrane manufacturer. The purity of the Au₁₁ final product was comparable to that of the crystalline Au11 standard purified by traditional means. These results suggest that a diafiltration system incorporating an organic solvent resistant nanofiltration membrane would be practical for rapid purification and high product recovery of gold nanoparticles in an organic solvent environment immediately downstream of a microreactor within a nanofactory architecture. Advisors/Committee Members: Remcho, Vincent T (advisor), Paul, Brian K (committee member).

Subjects/Keywords: microfluidic; Microfluidics

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APA (6^th Edition):

Rundel, J. T. (2008). Design, fabrication and application of a microfluidic nanofiltration module for separation and purification of macromolecules and nanoparticles. (Doctoral Dissertation). Oregon State University. Retrieved from http://hdl.handle.net/1957/7682

Chicago Manual of Style (16^th Edition):

Rundel, Jack T. “Design, fabrication and application of a microfluidic nanofiltration module for separation and purification of macromolecules and nanoparticles.” 2008. Doctoral Dissertation, Oregon State University. Accessed February 25, 2020. http://hdl.handle.net/1957/7682.

MLA Handbook (7^th Edition):

Rundel, Jack T. “Design, fabrication and application of a microfluidic nanofiltration module for separation and purification of macromolecules and nanoparticles.” 2008. Web. 25 Feb 2020.

Vancouver:

Rundel JT. Design, fabrication and application of a microfluidic nanofiltration module for separation and purification of macromolecules and nanoparticles. [Internet] [Doctoral dissertation]. Oregon State University; 2008. [cited 2020 Feb 25]. Available from: http://hdl.handle.net/1957/7682.

Council of Science Editors:

Rundel JT. Design, fabrication and application of a microfluidic nanofiltration module for separation and purification of macromolecules and nanoparticles. [Doctoral Dissertation]. Oregon State University; 2008. Available from: http://hdl.handle.net/1957/7682

University of KwaZulu-Natal

5. Moodley, Revesa. Evaluation of paper substrates for microfluidic application in medical diagnostic kits.

Degree: 2018, University of KwaZulu-Natal

URL: https://researchspace.ukzn.ac.za/handle/10413/16613

► Recent studies in the biomedical field have shown the use of paper in microfluidic analytical devices. However, no studies have been undertaken to ascertain what… (more)

▼ Recent studies in the biomedical field have shown the use of paper in microfluidic analytical devices. However, no studies have been undertaken to ascertain what type(s) of paper substrates are ideal for such microfluidic applications. Hence, this study was conducted to determine the feasibility of using different paper substrates for implementation in microfluidic analytical devices, specifically in the South African context, compared to currently used materials such as glass and silicone. In addition, fibres in paper substrates were substituted with nanofibrillated cellulose (NFC) fibres to ascertain the impact of NFC on the microfluidics of the paper substrates. The wicking rate of the substrates was the focus of the study, with high -resolution field emission gun-scanning electron microscopy and contact angle tests being used to support the results obtained. Solid wax printing was also conducted to determine whether the paper substrates were suited to fabrication for microfluidic applications. High-resolution morphological studies of the paper substrates showed that pore sizes of the pulp fibres were in the order sulphite> bleached kraft > unbleached kraft > Whatman No. 1 Chromatography Filter Paper > Whatman 3MM Chromatography Filter Paper > thermo-mechanical > recycled. It was concluded that the larger pore size of fibres correlated with faster wicking rates of the relevant paper substrates. The substitution of pulp fibres with NFC led to reduced pore size of the fibres thus leading to reduced wicking rates due to the presence of the small NFC particles. Contact angle is directly linked to the hydrophobicity of the substrate and is indicative of the resistance to absorption of a liquid. The results revealed that all the paper substrates were hydrophilic. However, the hydrophilicity of two of the substrates (sheets substituted with 100% NFC unbleached kraft pulp and 100% NFC recycled pulp) were higher than those of the other substrates indicating that, although these substrates were still hydrophilic in nature, their absorption of aqueous liquid would take longer periods of time. The results showed that Whatman glass microfiber GF/D filter paper was the fastest wicking substrate, using both dye and blood simulant as wicking liquids. Similarly, paper substrates made with recycled fibres exhibited the slowest wicking rates when using both wicking liquids. These results can be used when determining which of the substrates to use for paper-based microfluidic device (μPAD) application, whereby the desired detection time would be the factor used to establish which of the substrates to use. Comparison of vertical and horizontal tests showed varying results. In theory, the horizontal wicking test should result in a faster wicking rate than the vertical test, taking hydrostatic pressure into consideration. The majority of the substrates showed that the horizontal wicking rate was faster when using the dye solution, whereas vertical wicking was faster when using the blood simulant. Discrepancies between the results… Advisors/Committee Members: Land, Kevin. (advisor), Sithole, Bruce. (advisor), van Zyl, Werner. (advisor), Andrew, Jerome. (advisor).

Subjects/Keywords: MICROFLUIDIC.; DIAGNOSTIC.

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APA (6^th Edition):

Moodley, R. (2018). Evaluation of paper substrates for microfluidic application in medical diagnostic kits. (Thesis). University of KwaZulu-Natal. Retrieved from https://researchspace.ukzn.ac.za/handle/10413/16613

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Moodley, Revesa. “Evaluation of paper substrates for microfluidic application in medical diagnostic kits.” 2018. Thesis, University of KwaZulu-Natal. Accessed February 25, 2020. https://researchspace.ukzn.ac.za/handle/10413/16613.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Moodley, Revesa. “Evaluation of paper substrates for microfluidic application in medical diagnostic kits.” 2018. Web. 25 Feb 2020.

Vancouver:

Moodley R. Evaluation of paper substrates for microfluidic application in medical diagnostic kits. [Internet] [Thesis]. University of KwaZulu-Natal; 2018. [cited 2020 Feb 25]. Available from: https://researchspace.ukzn.ac.za/handle/10413/16613.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Moodley R. Evaluation of paper substrates for microfluidic application in medical diagnostic kits. [Thesis]. University of KwaZulu-Natal; 2018. Available from: https://researchspace.ukzn.ac.za/handle/10413/16613

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Texas A&M University

6. Sobahi, Nebras MohammedKamal A. Development of High-Throughput Microfluidic Impedance Spectroscopy Platform for Analyzing Microdroplets in Droplet Microfluidic System.

Degree: MS, Electrical Engineering, 2014, Texas A&M University

URL: http://hdl.handle.net/1969.1/153517

► This thesis presents the development of a high-throughput microfluidic impedance spectroscopy platform for electrically detecting analyzing impedance measurements of non-contact and label free microdroplets. This… (more)

Subjects/Keywords: microfluidic; impedance

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APA (6^th Edition):

Sobahi, N. M. A. (2014). Development of High-Throughput Microfluidic Impedance Spectroscopy Platform for Analyzing Microdroplets in Droplet Microfluidic System. (Masters Thesis). Texas A&M University. Retrieved from http://hdl.handle.net/1969.1/153517

Chicago Manual of Style (16^th Edition):

Sobahi, Nebras MohammedKamal A. “Development of High-Throughput Microfluidic Impedance Spectroscopy Platform for Analyzing Microdroplets in Droplet Microfluidic System.” 2014. Masters Thesis, Texas A&M University. Accessed February 25, 2020. http://hdl.handle.net/1969.1/153517.

MLA Handbook (7^th Edition):

Sobahi, Nebras MohammedKamal A. “Development of High-Throughput Microfluidic Impedance Spectroscopy Platform for Analyzing Microdroplets in Droplet Microfluidic System.” 2014. Web. 25 Feb 2020.

Vancouver:

Sobahi NMA. Development of High-Throughput Microfluidic Impedance Spectroscopy Platform for Analyzing Microdroplets in Droplet Microfluidic System. [Internet] [Masters thesis]. Texas A&M University; 2014. [cited 2020 Feb 25]. Available from: http://hdl.handle.net/1969.1/153517.

Council of Science Editors:

Sobahi NMA. Development of High-Throughput Microfluidic Impedance Spectroscopy Platform for Analyzing Microdroplets in Droplet Microfluidic System. [Masters Thesis]. Texas A&M University; 2014. Available from: http://hdl.handle.net/1969.1/153517

Rutgers University

7. Prakash, Shreya, 1994-. A microfluidic biosensing architecture for multiplexed analyte detection using hydrogel barcoded particles.

Degree: MS, Electrical and Computer Engineering, 2019, Rutgers University

URL: https://rucore.libraries.rutgers.edu/rutgers-lib/61911/

► Multiplexing is a method of analyzing multiple analytes in a biological assay in a single step. It provides advantage of shorter processing time, low sample… (more)

▼ Multiplexing is a method of analyzing multiple analytes in a biological assay in a single step. It provides advantage of shorter processing time, low sample volume and reduced cost per test. Currently, Flow Cytometers are used for blood cells enumeration, however, they are expensive, requires fluorescent tagging of cells and trained technicians to operate the instrument. We propose a non-fluorescent microfluidic architecture with single excitation and detection scheme using the barcoded particles fabricated using Stop Flow Lithography process. The barcoded particles designed with specific number of coding regions can generate numerous distinct patterns of electrical signatures and can be used in biological assays to detect multiple blood cells. A novel design of the asymmetric rectangular barcoded particle was proposed and tested in COMSOL Multiphysics. The barcoded particle with five coding regions generated distinct bi-polar signatures in the microfluidic impedance detection system. Different configurations of the sensing system were proposed to detect the conjugated microspheres (representative of blood cells) effectively based on the site of conjugation. The bottom co-planar electrode and top-bottom electrode configurations were tested for sensitive detection of conjugated microspheres to the barcoded particle. We found that our microfluidic detection system was sensitive enough to detect the presence of a microsphere attached to the barcoded particle. Further, we also investigated the effect of microspheres conjugation orientation to the barcoded particle and their associated electrical signatures. We developed a multi-feature selection algorithm to solve the orientation problem and improved the accuracy of our sensing mechanism. Finally, we fabricated the microfluidic chips using lithography, and co-planar electrodes on glass surfaces using thin film deposition process in the clean room. Our proposed microfluidic system can enumerate multiple blood cells in a single assay using barcoded particles. The concentration of these cells provides useful information about disease onset and progression. Such sensors can be used for diagnostic and management of diseases like Sepsis, Acute Kidney Injury, HIV/ AIDS. Advisors/Committee Members: Hassan, Umer (chair), Gajic, Zoran (internal member), Wu, Chung-Tse Michael (internal member), School of Graduate Studies.

Subjects/Keywords: Microfluidic; Biosensors

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APA (6^th Edition):

Prakash, Shreya, 1. (2019). A microfluidic biosensing architecture for multiplexed analyte detection using hydrogel barcoded particles. (Masters Thesis). Rutgers University. Retrieved from https://rucore.libraries.rutgers.edu/rutgers-lib/61911/

Chicago Manual of Style (16^th Edition):

Prakash, Shreya, 1994-. “A microfluidic biosensing architecture for multiplexed analyte detection using hydrogel barcoded particles.” 2019. Masters Thesis, Rutgers University. Accessed February 25, 2020. https://rucore.libraries.rutgers.edu/rutgers-lib/61911/.

MLA Handbook (7^th Edition):

Prakash, Shreya, 1994-. “A microfluidic biosensing architecture for multiplexed analyte detection using hydrogel barcoded particles.” 2019. Web. 25 Feb 2020.

Vancouver:

Prakash, Shreya 1. A microfluidic biosensing architecture for multiplexed analyte detection using hydrogel barcoded particles. [Internet] [Masters thesis]. Rutgers University; 2019. [cited 2020 Feb 25]. Available from: https://rucore.libraries.rutgers.edu/rutgers-lib/61911/.

Council of Science Editors:

Prakash, Shreya 1. A microfluidic biosensing architecture for multiplexed analyte detection using hydrogel barcoded particles. [Masters Thesis]. Rutgers University; 2019. Available from: https://rucore.libraries.rutgers.edu/rutgers-lib/61911/

Oregon State University

8. Koesdjojo, Myra T. Fabrication and application of polymer based microfluidic devices.

Degree: PhD, Chemistry, 2009, Oregon State University

URL: http://hdl.handle.net/1957/10841

► The concept of reducing laboratory operations in scale such that they fit on a microfluidic chip has been met with great enthusiasm. Lab-on-a-chip devices promise… (more)

▼ The concept of reducing laboratory operations in scale such that they fit on a microfluidic chip has been met with great enthusiasm. Lab-on-a-chip devices promise to be cost effective to operate due to reduced reagent consumption, have the potential to offer shorter analysis times due to their short path lengths, and may be useful in biological applications in that they are inherently compact and inexpensive to build, thus they may be disposable. In this work, a series of fabrication techniques for the production of polymer-based microfluidic devices are explored. In the first component of this research effort an aluminum mold was fabricated using CNC machining to create the desired microchannel design, followed by a two-stage embossing process, involving two polymer substrates with different glass transition temperatures (Tg), polyetherimide (PEI; Tg~216 °C) and poly(methyl methacrylate) (PMMA; Tg~105 °C). Successful feature transfer from aluminum mold to PMMA substrates was achieved reproducibly employing this method. With this approach, the expensive process of producing the aluminum master need be performed only once. Electrophoretic separations of fluorescent dyes, rhodamine B and fluorescein were performed on the PMMA microchips, with peak efficiencies of 55500 and 66300 theoretical plates/meter, respectively. The next stage of work explored a new bonding method by solvent welding using ice as sacrificial layer to prevent channel deformation. Water is one of the most compatible sacrificial media; it is readily available, non toxic, has a low evaporation rate, a high freezing point relative to the bonding solvent, and a low melting point which makes it easier to flush out after sealing, as compared to using other sacrificial media (paraffin wax or low-melting temperature alloys). The bonded PMMA microchips could withstand an internal pressure of > 2000 psi, more than 17 times stronger than the thermally bonded chips. In the final stage of work a new bonding technique was developed that readily produces complete microfluidic chips, without the need of a sacrificial layer to form complete multilayer microfluidic devices. Also developed was the use of an SU-8 master in the two-stage embossing process to create microchannels. This approach is faster, simpler and less costly than CNC machining. The fabrication technique was utilized to build a microfluidic liquid chromatography (LC) system that was shown to generate high separation efficiencies of 10,000- 45,000 plates/m. In addition, a passive micromixer containing high-density microfeatures was fabricated to perform a glycine assay using O-phthaldialdehyde. With glycine concentrations ranging from 0.0 to 2.6 μM, a linear calibration plot (R2 = 0.9982) was obtained with a detection limit of 0.032 μM. Advisors/Committee Members: Remcho, Vincent (advisor), Chang, Chih-hung (committee member).

Subjects/Keywords: microfluidic; Microfluidic devices – Design and construction

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APA (6^th Edition):

Koesdjojo, M. T. (2009). Fabrication and application of polymer based microfluidic devices. (Doctoral Dissertation). Oregon State University. Retrieved from http://hdl.handle.net/1957/10841

Chicago Manual of Style (16^th Edition):

Koesdjojo, Myra T. “Fabrication and application of polymer based microfluidic devices.” 2009. Doctoral Dissertation, Oregon State University. Accessed February 25, 2020. http://hdl.handle.net/1957/10841.

MLA Handbook (7^th Edition):

Koesdjojo, Myra T. “Fabrication and application of polymer based microfluidic devices.” 2009. Web. 25 Feb 2020.

Vancouver:

Koesdjojo MT. Fabrication and application of polymer based microfluidic devices. [Internet] [Doctoral dissertation]. Oregon State University; 2009. [cited 2020 Feb 25]. Available from: http://hdl.handle.net/1957/10841.

Council of Science Editors:

Koesdjojo MT. Fabrication and application of polymer based microfluidic devices. [Doctoral Dissertation]. Oregon State University; 2009. Available from: http://hdl.handle.net/1957/10841

Louisiana State University

9. De Guzman, Jennifer Macalindong. Fabrication of Receptor-Modified Microfluidic Surfaces for Applications in Glycoprotein Screening.

Degree: PhD, Chemistry, 2010, Louisiana State University

URL: etd-11152010-112728 ; https://digitalcommons.lsu.edu/gradschool_dissertations/3084

► Glycoproteins have long been identified to have a profound association with human pathological processes, and they are much sought after as potential biomarkers to aid… (more)

▼ Glycoproteins have long been identified to have a profound association with human pathological processes, and they are much sought after as potential biomarkers to aid in the early diagnosis and clinical prognosis of cancers and diseases. There is currently high demand for high-throughput and low–limit–of–detection techniques that can afford profiling of the glycoproteome. Micro-total analysis systems (µTAS) based on microfluidics have the potential to fulfill these requirements, but in order to reduce the complexity of the protein pool, the µTAS devices must contain a pre-isolation and enrichment component. The research project undertaken here involved derivatization of microfluidic surfaces with ligands to allow for capture and isolation of glycoproteins in solution. It is envisioned that a microfluidic device operating in a serial affinity mode can be fabricated whereby a large set of glycoproteins are captured by a global capture element, followed by further fractionation of the previously captured glycoprotein pool into unique glycoproteins by capture elements specific to each unique protein. To that end, the research here involved (1) modification of poly(methyl methacrylate) surfaces with a boronic acid derivative as the global glycoprotein receptor and (2) investigation of a surface-amenable synthetic route for the creation of a thermoresponsive scaffold with immobilized lectin, as the specific glycoprotein receptor, and its complementary eluting sugar. Creation of these surfaces is the first step toward realizing a µTAS for glycoprotein analysis. The novel boronic acid derivative 4-[(2-aminoethyl)carbamoyl]phenylboronic acid was immobilized on carboxymethyl dextran surfaces, and its protein interaction analysis was investigated by surface plasmon resonance spectroscopy. Poly(methyl methacrylate) microfluidic surfaces were then functionalized with the novel boronic acid derivative to yield a first-generation global capture modality. Glycoprotein binding to and elution from the global capture surface was afforded using glycine- and Tris-binding buffer systems and borate-eluting buffer systems, respectively, with the aid of Tween 20. A thermoresponsive terpolymer poly(N-isopropylacrylamide–lactose–RCA₁₂₀), with the lectin Ricinus communis agglutinin (RCA₁₂₀) as the specific capture element, was successfully prepared by surface-amenable synthetic protocols. The synthetic strategy proposed in this work can be easily adapted in the creation of microfluidic devices that can afford the capture of specific glycoproteins.

Subjects/Keywords: microfluidic surfaces; glycoproteins

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APA (6^th Edition):

De Guzman, J. M. (2010). Fabrication of Receptor-Modified Microfluidic Surfaces for Applications in Glycoprotein Screening. (Doctoral Dissertation). Louisiana State University. Retrieved from etd-11152010-112728 ; https://digitalcommons.lsu.edu/gradschool_dissertations/3084

Chicago Manual of Style (16^th Edition):

De Guzman, Jennifer Macalindong. “Fabrication of Receptor-Modified Microfluidic Surfaces for Applications in Glycoprotein Screening.” 2010. Doctoral Dissertation, Louisiana State University. Accessed February 25, 2020. etd-11152010-112728 ; https://digitalcommons.lsu.edu/gradschool_dissertations/3084.

MLA Handbook (7^th Edition):

De Guzman, Jennifer Macalindong. “Fabrication of Receptor-Modified Microfluidic Surfaces for Applications in Glycoprotein Screening.” 2010. Web. 25 Feb 2020.

Vancouver:

De Guzman JM. Fabrication of Receptor-Modified Microfluidic Surfaces for Applications in Glycoprotein Screening. [Internet] [Doctoral dissertation]. Louisiana State University; 2010. [cited 2020 Feb 25]. Available from: etd-11152010-112728 ; https://digitalcommons.lsu.edu/gradschool_dissertations/3084.

Council of Science Editors:

De Guzman JM. Fabrication of Receptor-Modified Microfluidic Surfaces for Applications in Glycoprotein Screening. [Doctoral Dissertation]. Louisiana State University; 2010. Available from: etd-11152010-112728 ; https://digitalcommons.lsu.edu/gradschool_dissertations/3084

Oregon State University

10. Tennico, Yolanda H. Magnetic particles for selective extraction of trace analytes in microfluidic devices.

Degree: PhD, Chemistry, 2010, Oregon State University

URL: http://hdl.handle.net/1957/14783

► The development of micro total analysis systems (µTAS), also called “lab-on-a-chip”, or microfluidic analysis systems, is presented in this dissertation. Various research areas, covering subjects… (more)

▼ The development of micro total analysis systems (µTAS), also called “lab-on-a-chip”, or microfluidic analysis systems, is presented in this dissertation. Various research areas, covering subjects from magnetic particles synthesis to novel microchip fabrication techniques, are explored to develop a lab-on-a-chip system capable of performing magnetic bead-based bioassays. These devices are proven not only to be cost-effective (due to their reduced reagent consumption), but also time-efficient (shorter analysis time), and may be disposable (they are inherently compact and inexpensive to produce), making them very attractive to be used in biomedical applications. The research starts with the utilization of functionalized magnetic particles as sorbents for an in-line extraction in confines of capillary columns. The synthesized silica-coated iron oxide particles functionalized with C18 groups were used as reversed-phase sorbents. Magnets were used to locally immobilize these particles inside the capillary. The results showed that extraction, elution, and detection of the analytes were performed sequentially without interruption or need for sample handling. The work continued with the development of bonding techniques for creating polymer-based microfluidic devices through surface modification process. This technique readily produced complete microfluidic chips via plasma oxidation followed by silane reagent treatment (tetraethyl orthosilicate) to facilitate siloxane bonds between the two polymers. It provides a versatile approach to bond dissimilar polymer substrates and between a hard polymer substrate to polydimethylsiloxane or glass. The two previous studies were combined allowing for the integration of magnetic particles in a microfluidic chip format. The benefits of each component were utilized to develop an on-chip aptamer-based fluorescence assay for thrombin detection and quantification based on sandwich ELISA principles. Aptamer-functionalized magnetic beads were utilized to capture the target analyte, while a second aptamer, functionalized with quantum dots, was employed for on-chip detection. Disposable microfluidic devices were employed as the platform to enable rapid and sensitive thrombin detection with the benefits of reduced costs, minimized material consumption, and simplified washing process. This work has opened up a new chapter in the development of magnetic beads-based materials and devices in a wide variety of applications for detection of target proteins of biomedical importance. Advisors/Committee Members: Remcho, Vincent T. (advisor), Lerner, Michael (committee member).

Subjects/Keywords: microfluidics; Microfluidic devices

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APA (6^th Edition):

Tennico, Y. H. (2010). Magnetic particles for selective extraction of trace analytes in microfluidic devices. (Doctoral Dissertation). Oregon State University. Retrieved from http://hdl.handle.net/1957/14783

Chicago Manual of Style (16^th Edition):

Tennico, Yolanda H. “Magnetic particles for selective extraction of trace analytes in microfluidic devices.” 2010. Doctoral Dissertation, Oregon State University. Accessed February 25, 2020. http://hdl.handle.net/1957/14783.

MLA Handbook (7^th Edition):

Tennico, Yolanda H. “Magnetic particles for selective extraction of trace analytes in microfluidic devices.” 2010. Web. 25 Feb 2020.

Vancouver:

Tennico YH. Magnetic particles for selective extraction of trace analytes in microfluidic devices. [Internet] [Doctoral dissertation]. Oregon State University; 2010. [cited 2020 Feb 25]. Available from: http://hdl.handle.net/1957/14783.

Council of Science Editors:

Tennico YH. Magnetic particles for selective extraction of trace analytes in microfluidic devices. [Doctoral Dissertation]. Oregon State University; 2010. Available from: http://hdl.handle.net/1957/14783

Oregon State University

11. Nammoonnoy, Jintana. A photoactivable microfluidic device for heavy metal ion extraction.

Degree: PhD, Chemistry, 2010, Oregon State University

URL: http://hdl.handle.net/1957/18461

► The development of microfluidic devices for heavy metal extraction is presented in this dissertation. Various research areas, covering subjects from photochromic compound syntheses to microchip… (more)

▼ The development of microfluidic devices for heavy metal extraction is presented in this dissertation. Various research areas, covering subjects from photochromic compound syntheses to microchip fabrication techniques are explored to develop microfluidic devices capable of extracting heavy metal ions from drinking water. Through integration of the beneficial characteristics of both microfluidic devices and photochromic dyes, a simple and innovative microchip configured as a photoactivable extraction system for metal ion accumulation and release can be realized. The initial research focused on the utilization of photochromic compounds namely spiropyrans, as chelators for heavy metal extraction. Spiropyrans are organic photochromic compounds that have been widely studied. Upon irradiation with UV or visible light, spiropyrans isomerize between the closed and open forms, in which the open form is comparatively more polar. Metal ions can influence this isomerization process by associating with the open form through the electron-rich oxygen atom. In contrast, visible light produces a high concentration of the closed form, and thus hinders metal-binding. Spiropyrans, therefore, show great potential as photo-reversible metal-complexation agents. The spiropyran derivatives were synthesized and immobilized on solid supports including polymeric resins and poly(methylmethacrylate) (PMMA) microchips. Metal ion uptake can be triggered using UV light and subsequently reversed on demand by shining green light on the colored complex, which regenerates the inactive spiropyran form resulting in the release of metal ions. The use of light to trigger the chelator offers unique opportunities. The work continued with the development of microfluidic fabrication for creating versatile, solvent-compatible microfluidic devices through surface modification. There are challenges associated with the use of polymeric based microfluidic devices, particularly in surface modification steps, as solvents can embrittle thermoplastics that resulting in microcracking. To overcome this limitation, we explored the possibility of fabricating extremely robust microfluidic devices entirely from fluorocarbon and glass materials. The work demonstrated a new fabrication technique based on a chemically activated poly(tetrafluoro ethylene) (PTFE) sheet sandwiched between chemically activated glass substrates. The PTFE microchannels can be fabricated in minutes using a cutting plotter to create microchannels. The possibility of glass to PTFE makes this method applicable in a wide range of applications. Advisors/Committee Members: Remcho, Vincent T. (advisor), Walker, Jeffrey R. (committee member).

Subjects/Keywords: Microfluidics; Microfluidic devices

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APA (6^th Edition):

Nammoonnoy, J. (2010). A photoactivable microfluidic device for heavy metal ion extraction. (Doctoral Dissertation). Oregon State University. Retrieved from http://hdl.handle.net/1957/18461

Chicago Manual of Style (16^th Edition):

Nammoonnoy, Jintana. “A photoactivable microfluidic device for heavy metal ion extraction.” 2010. Doctoral Dissertation, Oregon State University. Accessed February 25, 2020. http://hdl.handle.net/1957/18461.

MLA Handbook (7^th Edition):

Nammoonnoy, Jintana. “A photoactivable microfluidic device for heavy metal ion extraction.” 2010. Web. 25 Feb 2020.

Vancouver:

Nammoonnoy J. A photoactivable microfluidic device for heavy metal ion extraction. [Internet] [Doctoral dissertation]. Oregon State University; 2010. [cited 2020 Feb 25]. Available from: http://hdl.handle.net/1957/18461.

Council of Science Editors:

Nammoonnoy J. A photoactivable microfluidic device for heavy metal ion extraction. [Doctoral Dissertation]. Oregon State University; 2010. Available from: http://hdl.handle.net/1957/18461

Cornell University

12. Santana, Steven. Microfluidic Analysis Of Metastatic Cancer Biomarkers .

Degree: 2014, Cornell University

URL: http://hdl.handle.net/1813/38925

► Cancer is the second-leading cause of death in the United States. Metastasis is responsible for 90% of cancer-related death and progresses through multifarious, poorly-understood cascades… (more)

▼ Cancer is the second-leading cause of death in the United States. Metastasis is responsible for 90% of cancer-related death and progresses through multifarious, poorly-understood cascades as they are difficult to observe in vivo. It is widely held that deciphering the metastatic cascade and identifying metastatic precursors will lead to improved patient outcomes. In this work, we isolate and study cancer biomarkers, specifically circulating tumor cells (CTCs) and cancer-cell-derived extracellular shed vesicles (ESVs), implicated in cancer progression and metastasis. First we describe the design, fabrication, and use of a Hele-Shaw microfluidic system to optimize rarecell immunocapture parameters with a focus on informing the design of systems for CTC isolation from patient whole blood. Our study includes the role of antibody selection, density, antigen locations, and multi-modal capture surfaces, as well as shear stress, on rare cell capture. We use LNCaPs, a PSMA-expressing prostate cancer cell line, as a model for CTCs and anti-PSMA antibodies, J591 and J415, to inform chemistry-mediated immobilization. Next, we focus on another cancer-disseminated marker, extracellular shed vesicles. ESVs, including exosomes and cancer-cell-derived microvesicles, are disseminated throughout the body and represent an important conduit of cell communication. Microvesicles have potential as a cancer biomarker as they are believed to transform tumor microenvironments and prime the metastatic niche. Cancercell-derived ESV subpopulations consist of a small-diameter exosome population and a large-diameter, cancer-cell-specific microvesicle population, each formed by unique mechanisms. It is believed that size correlates with biological properties of interest, but isolating these subpopulations, to discern chemical, biological, or physical differences, is challenging. We designed a deterministic lateral displacement microfluidic platform to isolate a pure microvesicle sample from the heterogeneous cancer-cell-derived ESV population. The threshold diameter differentiating the microvesicle population from the exosome population was determined by characterizing the size distributions of ESVs harvested from multiple cancer cell lines of breast, brain, and pancreas origin. Our microvesicle-isolation microfluidic technology facilitates future investigations regarding microvesicles' role in cancer progression by enabling identification of cargo carried by the microvesicle subpopulation. Advisors/Committee Members: Fischbach, Claudia (committeeMember), Sipple, John W (committeeMember), Cerione, Richard A (committeeMember).

Subjects/Keywords: microfluidic; metastasis; biomarker

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APA (6^th Edition):

Santana, S. (2014). Microfluidic Analysis Of Metastatic Cancer Biomarkers . (Thesis). Cornell University. Retrieved from http://hdl.handle.net/1813/38925

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Santana, Steven. “Microfluidic Analysis Of Metastatic Cancer Biomarkers .” 2014. Thesis, Cornell University. Accessed February 25, 2020. http://hdl.handle.net/1813/38925.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Santana, Steven. “Microfluidic Analysis Of Metastatic Cancer Biomarkers .” 2014. Web. 25 Feb 2020.

Vancouver:

Santana S. Microfluidic Analysis Of Metastatic Cancer Biomarkers . [Internet] [Thesis]. Cornell University; 2014. [cited 2020 Feb 25]. Available from: http://hdl.handle.net/1813/38925.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Santana S. Microfluidic Analysis Of Metastatic Cancer Biomarkers . [Thesis]. Cornell University; 2014. Available from: http://hdl.handle.net/1813/38925

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Wisconsin – Milwaukee

13. Amin, Alaknanda. Microfluidic Device Integration of Electrostatic Corral Trapping Systems.

Degree: MS, Chemistry, 2014, University of Wisconsin – Milwaukee

URL: https://dc.uwm.edu/etd/519

► This thesis describes the development, characterization, and application of the microfluidics device integration of electrostatic corral trapping systems. Optical traps or "laser tweezers", which… (more)

Subjects/Keywords: Microfluidic; Trapping; Chemistry

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APA (6^th Edition):

Amin, A. (2014). Microfluidic Device Integration of Electrostatic Corral Trapping Systems. (Thesis). University of Wisconsin – Milwaukee. Retrieved from https://dc.uwm.edu/etd/519

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Amin, Alaknanda. “Microfluidic Device Integration of Electrostatic Corral Trapping Systems.” 2014. Thesis, University of Wisconsin – Milwaukee. Accessed February 25, 2020. https://dc.uwm.edu/etd/519.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Amin, Alaknanda. “Microfluidic Device Integration of Electrostatic Corral Trapping Systems.” 2014. Web. 25 Feb 2020.

Vancouver:

Amin A. Microfluidic Device Integration of Electrostatic Corral Trapping Systems. [Internet] [Thesis]. University of Wisconsin – Milwaukee; 2014. [cited 2020 Feb 25]. Available from: https://dc.uwm.edu/etd/519.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Amin A. Microfluidic Device Integration of Electrostatic Corral Trapping Systems. [Thesis]. University of Wisconsin – Milwaukee; 2014. Available from: https://dc.uwm.edu/etd/519

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Queens University

14. Swyer, Ian. Considerations on the use of Impedance Spectroscopy for the Detection of Virions Trapped in Quadrupolar Microelectrode Arrays .

Degree: Chemical Engineering, 2011, Queens University

URL: http://hdl.handle.net/1974/6528

► The impedance response of a quadrupolar microelectrode array was studied over a wide frequency range to determine whether particles captured at the center of the… (more)

▼ The impedance response of a quadrupolar microelectrode array was studied over a wide frequency range to determine whether particles captured at the center of the array could be detected impedimetrically. The microelectrode array (denoted as DEP chip) uses dielectrophoretic forces to concentrate particles at its center. Initial results showed that there was a large electrode-silicon-electrode (ESE) capacitance which dominated at high frequencies. This capacitance was reduced by decreasing the electrode area and increasing the insulating layer thickness. These measures however proved fruitless as this capacitance was still significantly greater then the dielectric capacitance of the chip. This ESE capacitance can be eliminated through the use of a glass substrate so that the dielectric response of the chip dominates at higher frequencies. Since the ESE capacitance prevented experimental validation of impedance spectroscopy as a signal transduction method, computer simulations were performed. These simulations indicated that capture with the current DEP chips would not have a significant impact on the impedance of the chip. Decreasing the electrode gap distance and reducing the area of the electrodes, which is recommended for future work, can remedy this. As measureable changes in the dielectric capacitance of the chip are not possible, a reaction scheme was developed to translate the capture of viral particles into a change in medium conductivity. An ELISA type system was proposed where the viral particles would be functionalized with urease. This uease would then be used to degrade non-ionic urea into ionic products thereby increasing the medium conductivity. A model was formulated to predict the conductivity increase expected for low concentrations, and validated using higher concentrations of biotinylated-urease. Urease from commercial sources proved not to be a viable option as it does not possess a high enough activity to produce a significant conductivity change given the low concentrations of viral particles expected after collection. Urease with suitable activity is produced by the organism Ureaplasma urealyticum which has an activity of 180 000 µmol urea catalyzed min-1 mg urease-1. It is not recommended that this method be pursued further due to technical challenges that would be encountered.

Subjects/Keywords: Impedance; Biosensors; Microfluidic

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APA (6^th Edition):

Swyer, I. (2011). Considerations on the use of Impedance Spectroscopy for the Detection of Virions Trapped in Quadrupolar Microelectrode Arrays . (Thesis). Queens University. Retrieved from http://hdl.handle.net/1974/6528

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Swyer, Ian. “Considerations on the use of Impedance Spectroscopy for the Detection of Virions Trapped in Quadrupolar Microelectrode Arrays .” 2011. Thesis, Queens University. Accessed February 25, 2020. http://hdl.handle.net/1974/6528.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Swyer, Ian. “Considerations on the use of Impedance Spectroscopy for the Detection of Virions Trapped in Quadrupolar Microelectrode Arrays .” 2011. Web. 25 Feb 2020.

Vancouver:

Swyer I. Considerations on the use of Impedance Spectroscopy for the Detection of Virions Trapped in Quadrupolar Microelectrode Arrays . [Internet] [Thesis]. Queens University; 2011. [cited 2020 Feb 25]. Available from: http://hdl.handle.net/1974/6528.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Swyer I. Considerations on the use of Impedance Spectroscopy for the Detection of Virions Trapped in Quadrupolar Microelectrode Arrays . [Thesis]. Queens University; 2011. Available from: http://hdl.handle.net/1974/6528

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Hong Kong University of Science and Technology

15. Tian, Jingxuan PHYS. A valve-free 2D concentration gradient generator.

Degree: 2016, Hong Kong University of Science and Technology

URL: http://repository.ust.hk/ir/Record/1783.1-97837 ; https://doi.org/10.14711/thesis-b1610623 ; http://repository.ust.hk/ir/bitstream/1783.1-97837/1/th_redirect.html

► This thesis mainly studies topics in microfluidic chips. Microfluidic chip is a promising platform for multiple discipline, like biology, medicine, chemistry and material synthesis. It… (more)

Subjects/Keywords: Microfluidic devices ; Microfluidics

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APA (6^th Edition):

Tian, J. P. (2016). A valve-free 2D concentration gradient generator. (Thesis). Hong Kong University of Science and Technology. Retrieved from http://repository.ust.hk/ir/Record/1783.1-97837 ; https://doi.org/10.14711/thesis-b1610623 ; http://repository.ust.hk/ir/bitstream/1783.1-97837/1/th_redirect.html

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Tian, Jingxuan PHYS. “A valve-free 2D concentration gradient generator.” 2016. Thesis, Hong Kong University of Science and Technology. Accessed February 25, 2020. http://repository.ust.hk/ir/Record/1783.1-97837 ; https://doi.org/10.14711/thesis-b1610623 ; http://repository.ust.hk/ir/bitstream/1783.1-97837/1/th_redirect.html.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Tian, Jingxuan PHYS. “A valve-free 2D concentration gradient generator.” 2016. Web. 25 Feb 2020.

Vancouver:

Tian JP. A valve-free 2D concentration gradient generator. [Internet] [Thesis]. Hong Kong University of Science and Technology; 2016. [cited 2020 Feb 25]. Available from: http://repository.ust.hk/ir/Record/1783.1-97837 ; https://doi.org/10.14711/thesis-b1610623 ; http://repository.ust.hk/ir/bitstream/1783.1-97837/1/th_redirect.html.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Tian JP. A valve-free 2D concentration gradient generator. [Thesis]. Hong Kong University of Science and Technology; 2016. Available from: http://repository.ust.hk/ir/Record/1783.1-97837 ; https://doi.org/10.14711/thesis-b1610623 ; http://repository.ust.hk/ir/bitstream/1783.1-97837/1/th_redirect.html

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Hong Kong University of Science and Technology

16. Wang, Jing. A new on-chip immunoassay platform combining yeast surface display and microfluidic system for point-of-care application.

Degree: 2011, Hong Kong University of Science and Technology

URL: http://repository.ust.hk/ir/Record/1783.1-7375 ; https://doi.org/10.14711/thesis-b1155745 ; http://repository.ust.hk/ir/bitstream/1783.1-7375/1/th_redirect.html

► Immunoassay, as a technique that utilizes antibody-antigen interactions for the detection of relevant analytes, plays an important role in our daily life. It can be… (more)

▼ Immunoassay, as a technique that utilizes antibody-antigen interactions for the detection of relevant analytes, plays an important role in our daily life. It can be used for the quantification of proteins and small molecules in a number of different fields, such as medical diagnosis, food safety and environmental monitoring. The analysis of antibodies directed against specific epitopes with high specificity and sensitivity in human serum is becoming an increasingly critical task in medical and life sciences as the process provides detection of a serologic biomarker which enables early diagnosis in many diseases: for example, infectious diseases such as HIV and hepatitis B, autoimmune diseases such as systemic lupus erythematosus (SLE), as well as allergies and cancers. Recently, there is an urgent global need for point-of-care immunoassays which can be operated by end users since early stage antibody detection plays a crucial role in disease diagnosis and treatment. In this thesis work, we developed a new on-chip immunoassay platform, which combines the yeast surface display (YSD) technique and a microfluidic system. By directly counting genetically-engineered yeast cells bound with target analytes, the immunoassay was realized on a microchip tailored for point-of-care applications. Taking advantage of YSD, antigenic reporters are easily to be obtained by simply yeast culture, and the tedious steps of antigen purification and wet-chemistry processes to immobilize probe antigen can be eliminated. At the same time, with individual cells readily counted by routine microscopy, fewer numbers of cells can be detected than fluorescence or chromophore-based methods. Thus, this method has the inherent characteristic to be highly sensitive. Furthermore, controlled laminar flow can be applied to preferentially remove nonspecifically bound cells, improving the selectivity of the assay. We have built a prototypical microfluidic immunoassay device incorporating functionalized planar surface and fluidic force discrimination, and demonstrated multiplexed antibody detection using bifunctional engineered yeast cells. The magnetic bead-assisted method, which utilizes protein G coupled magnetic beads to capture antibody-labeled cells, improves the sensitivity of the YSD-based microfluidic immunoassay by 100 times and provides a detection limit of 0.5 ng/ml. Alternatively, we have also developed a functionalized micro pillar array (MPA)-based microfluidic immunoassay, utilizing capillary force as the driven power to replace pumping. The high surface area of the MPA results in a high possibility for cells to contact and attach to the pillars, and provides a detection limit of 5 ng/ml. This method is believed to have excellent compatibility with common biopsy used in general hospitals, as well as high potential to be integrated into a portable system. In order to achieve real point-of-care antibody detection that can be operated by end users, we have developed a non-optical YSD based microfluidic immunoassay using micro Coulter particle…

Subjects/Keywords: Immunoassay ; Microfluidic devices

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APA (6^th Edition):

Wang, J. (2011). A new on-chip immunoassay platform combining yeast surface display and microfluidic system for point-of-care application. (Thesis). Hong Kong University of Science and Technology. Retrieved from http://repository.ust.hk/ir/Record/1783.1-7375 ; https://doi.org/10.14711/thesis-b1155745 ; http://repository.ust.hk/ir/bitstream/1783.1-7375/1/th_redirect.html

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Wang, Jing. “A new on-chip immunoassay platform combining yeast surface display and microfluidic system for point-of-care application.” 2011. Thesis, Hong Kong University of Science and Technology. Accessed February 25, 2020. http://repository.ust.hk/ir/Record/1783.1-7375 ; https://doi.org/10.14711/thesis-b1155745 ; http://repository.ust.hk/ir/bitstream/1783.1-7375/1/th_redirect.html.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Wang, Jing. “A new on-chip immunoassay platform combining yeast surface display and microfluidic system for point-of-care application.” 2011. Web. 25 Feb 2020.

Vancouver:

Wang J. A new on-chip immunoassay platform combining yeast surface display and microfluidic system for point-of-care application. [Internet] [Thesis]. Hong Kong University of Science and Technology; 2011. [cited 2020 Feb 25]. Available from: http://repository.ust.hk/ir/Record/1783.1-7375 ; https://doi.org/10.14711/thesis-b1155745 ; http://repository.ust.hk/ir/bitstream/1783.1-7375/1/th_redirect.html.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Wang J. A new on-chip immunoassay platform combining yeast surface display and microfluidic system for point-of-care application. [Thesis]. Hong Kong University of Science and Technology; 2011. Available from: http://repository.ust.hk/ir/Record/1783.1-7375 ; https://doi.org/10.14711/thesis-b1155745 ; http://repository.ust.hk/ir/bitstream/1783.1-7375/1/th_redirect.html

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Utah

17. Booth, Ross Hunter. A microfluidic in vitro model of the blood-brain barrier.

Degree: PhD, Bioengineering, 2014, University of Utah

URL: http://content.lib.utah.edu/cdm/singleitem/collection/etd3/id/3355/rec/53

► The blood-brain barrier (BBB) limits entry of most molecules into the brain and complicates the development of brain-targeting compounds, necessitating novel BBB models. This dissertation… (more)

▼ The blood-brain barrier (BBB) limits entry of most molecules into the brain and complicates the development of brain-targeting compounds, necessitating novel BBB models. This dissertation describes the first microfluidic BBB model allowing the study of BBB properties in relation to various chemical compounds by enabling tunable wall shear stress (WSS) via dynamic fluid flow, cell-cell interaction through a thin co-culture membrane, time-dependent delivery of test compounds, and integration of sensors into the system, resulting in significant reduction of reagents and cells required and shorter cell seeding time. Use of parallel channels first enabled simultaneous monitoring of multiple cell populations under a wide range (~x15) of WSS.The microfluidic model formed the BBB by incorporating brain endothelial (b.End3) and glial (C6/C8D1A) cells at the intersection of two crossing microchannels, respectively representing luminal and abluminal sides, fabricated in a transparent polydimethylsiloxane (PDMS) substrate utilizing high-precision soft lithography techniques. The utilized cells were adopted from immortalized cells for high consistency over repeated passages and pure and proliferative culture.The developed microfluidic BBB model was validated by (1) expression of tight junction protein ZO-1 and glial protein GFAP by fluorescence imaging, and P-gp activity by Calcein AM, confirming key BBB proteins; (2) high trans-endothelial electrical resistance (TEER) of co-cultures exceeding 250Ωcm2 confirming sufficiently contiguous cell layer formation; (3) chemically-induced barrier modulation, with transient TEER loss by 150μM histamine (~50% for 8-15min), and increase in permeability at elevated pH (10.0); (4) size-dependent (668-70,000Da) compound permeability mimicking in vivo trends; and (5) highly linear correlation (R2>0.85) of clearance rates of seven selected neural drugs with in vivo brain/plasma ratios. We demonstrated the effects of WSS (0-86dyn/cm2) on bEnd.3 properties under increasing WSS, including increase in (6) TEER, (7) cell re-alignment toward flow direction, and (8) protein expression of ZO-1/P-gp, and (9) decrease in tracer permeability.The developed in vitro microfluidic BBB model provides distinct advantages for monitoring and modulating barrier functions and prediction of compound permeability. Thus, it would provide an innovative platform to study mechanisms and pathology of barrier function as well as to assess novel pharmaceuticals early in development for their BBB clearance capabilities.

Subjects/Keywords: Blood-brain barrier; MEMS; Microfluidic

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Booth, R. H. (2014). A microfluidic in vitro model of the blood-brain barrier. (Doctoral Dissertation). University of Utah. Retrieved from http://content.lib.utah.edu/cdm/singleitem/collection/etd3/id/3355/rec/53

Chicago Manual of Style (16^th Edition):

Booth, Ross Hunter. “A microfluidic in vitro model of the blood-brain barrier.” 2014. Doctoral Dissertation, University of Utah. Accessed February 25, 2020. http://content.lib.utah.edu/cdm/singleitem/collection/etd3/id/3355/rec/53.

MLA Handbook (7^th Edition):

Booth, Ross Hunter. “A microfluidic in vitro model of the blood-brain barrier.” 2014. Web. 25 Feb 2020.

Vancouver:

Booth RH. A microfluidic in vitro model of the blood-brain barrier. [Internet] [Doctoral dissertation]. University of Utah; 2014. [cited 2020 Feb 25]. Available from: http://content.lib.utah.edu/cdm/singleitem/collection/etd3/id/3355/rec/53.

Council of Science Editors:

Booth RH. A microfluidic in vitro model of the blood-brain barrier. [Doctoral Dissertation]. University of Utah; 2014. Available from: http://content.lib.utah.edu/cdm/singleitem/collection/etd3/id/3355/rec/53

University of Alberta

18. Olson, Steven. Charge transport in molecular junctions and microfluidic devices.

Degree: MS, Department of Physics, 2010, University of Alberta

URL: https://era.library.ualberta.ca/files/gx41mj807

► Electro-transmittance of molecular junctions was characterized electrically and studied optically at 410nm and 532nm. Between 1kHz and 100kHz there was no qualitative difference between the… (more)

Subjects/Keywords: microfluidic; molecular junctions; charge transport

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APA (6^th Edition):

Olson, S. (2010). Charge transport in molecular junctions and microfluidic devices. (Masters Thesis). University of Alberta. Retrieved from https://era.library.ualberta.ca/files/gx41mj807

Chicago Manual of Style (16^th Edition):

Olson, Steven. “Charge transport in molecular junctions and microfluidic devices.” 2010. Masters Thesis, University of Alberta. Accessed February 25, 2020. https://era.library.ualberta.ca/files/gx41mj807.

MLA Handbook (7^th Edition):

Olson, Steven. “Charge transport in molecular junctions and microfluidic devices.” 2010. Web. 25 Feb 2020.

Vancouver:

Olson S. Charge transport in molecular junctions and microfluidic devices. [Internet] [Masters thesis]. University of Alberta; 2010. [cited 2020 Feb 25]. Available from: https://era.library.ualberta.ca/files/gx41mj807.

Council of Science Editors:

Olson S. Charge transport in molecular junctions and microfluidic devices. [Masters Thesis]. University of Alberta; 2010. Available from: https://era.library.ualberta.ca/files/gx41mj807

University of Alberta

19. Zhang, Le. Functionalized Bead Based Microchip for Immunoassay and Virus Immuno-affinity Chromatography.

Degree: MS, Department of Chemistry, 2014, University of Alberta

URL: https://era.library.ualberta.ca/files/cth83kz515

► We demonstrate the functionalization of a highly ordered porous molecular sieving matrix created by colloidal self-assembly (CSA) of 2 micrometer diameter silica particles in microfluidic… (more)

▼ We demonstrate the functionalization of a highly ordered porous molecular sieving matrix created by colloidal self-assembly (CSA) of 2 micrometer diameter silica particles in microfluidic chips, for highly efficient immuno-capture of viruses. By tuning the particle size, with appropriate surface chemistry, we can easily match the pore size which could maximize biological species-particle wall collision, leading high capture efficiency. The ordered uniform lattice of pores, which are 15 % of the size of the particles used, should prove ideal for the capture of viruses (on the 50-150 nm scale in size). We report on characterization of capture beds with 300 nm pores. These beds were used to detect fluorescein using immobilized anti-fluorescein on the bed, and also to capture and detect type-5 adenovirus in suspension, and in infected cells using appropriate antibodies. We demonstrate the performance of the concept using a fully packed column for fluorescein immunoassay. We used 2 μm carboxylated silica particles self-assembled into a 6 mm long-bed, modified with EDC/NHS, and immobilized anti-fluorescein antibody or type-5 adenovirus-recognizing antibody. An electric field was applied to utilize electro-osmotic flow as the solvent pumping force. A 4.51 nM fluorescein solution was captured by immuno-affinity using anti-fluorescein antibody, and then released with an eluent to an empty downstream analysis region to give a very large signal, providing a positive control. Similar experiments were performed with type-5 adenovirus, demonstrating detection at a concentration of 8.3 × 103 viral particles (VP) per milliliter. Adenovirus in celllysate was also detected in this microchip, with the lowest concentration detectable at 1.5 × 103 PFU/mL. Combination of advanced biological detection methods with microfluidics based extraction and concentration techniques has tremendous potential for realization of a portable, cost effective and sensitive pathogen detection system. Taking advantage of the unique fluid flow characteristics of CSA structures on an even smaller scale than employed here, faster, more sensitive and more economical capture and pre-concentration techniques for diagnostic assays for a wider range of analytes can be developed.

Subjects/Keywords: Microfluidic; Immunoassay; Immuno-affinity Chromatography

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APA (6^th Edition):

Zhang, L. (2014). Functionalized Bead Based Microchip for Immunoassay and Virus Immuno-affinity Chromatography. (Masters Thesis). University of Alberta. Retrieved from https://era.library.ualberta.ca/files/cth83kz515

Chicago Manual of Style (16^th Edition):

Zhang, Le. “Functionalized Bead Based Microchip for Immunoassay and Virus Immuno-affinity Chromatography.” 2014. Masters Thesis, University of Alberta. Accessed February 25, 2020. https://era.library.ualberta.ca/files/cth83kz515.

MLA Handbook (7^th Edition):

Zhang, Le. “Functionalized Bead Based Microchip for Immunoassay and Virus Immuno-affinity Chromatography.” 2014. Web. 25 Feb 2020.

Vancouver:

Zhang L. Functionalized Bead Based Microchip for Immunoassay and Virus Immuno-affinity Chromatography. [Internet] [Masters thesis]. University of Alberta; 2014. [cited 2020 Feb 25]. Available from: https://era.library.ualberta.ca/files/cth83kz515.

Council of Science Editors:

Zhang L. Functionalized Bead Based Microchip for Immunoassay and Virus Immuno-affinity Chromatography. [Masters Thesis]. University of Alberta; 2014. Available from: https://era.library.ualberta.ca/files/cth83kz515

Texas A&M University

20. Balasubramanian, Ashwin Kumar. A microfluidic device for continuous capture and concentration of pathogens from water.

Degree: 2009, Texas A&M University

URL: http://hdl.handle.net/1969.1/ETD-TAMU-1339

► A microfluidic device, based on electrophoretic transport and electrostatic trapping of charged particles, has been developed for continuous capture and concentration of microorganisms from water.… (more)

▼ A microfluidic device, based on electrophoretic transport and electrostatic trapping of charged particles, has been developed for continuous capture and concentration of microorganisms from water. A generic design, utilizing mobility and zeta potential measurements of various microorganisms exposed to different environmental conditions and physiological states, was employed. Water and buffer samples at pH values ranging from 5.2?7.0 were seeded with bacteria (E. coli, Salmonella, and Pseudomonas) and viruses (MS-2 and Echovirus). Negative control and capture experiments were performed simultaneously using two identical devices. Both culture based methods and real-time PCR analysis were utilized to characterize the capture efficiency as a function of time, flowrate, and applied electric field. Based on differences between the capture and negative control data, capture efficiencies of 90% to 99% are reported for E. coli, Salmonella, Pseudomonas, and MS-2, while the capture efficiency for Echovirus was around 75%. Overall, the device exhibits 16.67 fold sample volume reduction within an hour at 6 mL/hr. This results in a concentration factor of 15 at 90% capture efficiency. Direct quantification of capture on the anode of the prototype microfluidic device was also performed by particle tracking using fluorescent microscopy. Based on image processing, the capture data at different locations on the electrode surface is quantified as a function of the wall shear stress at these locations, which is calculated using CFD simulations. Finally, the Faradaic processes in the microchannel due to electrochemical reactions are studied to predict the amount of electrophoresis in the system. Scaling of the device to sample 5 L/hr can be achieved by stacking 835 identical microchannels. Power and wetted volume for the prototype and scaled devices are presented. The device can thus function either as a filtration unit or as a sample concentrator to enable the application of real-time detection sensor technologies. The ability to continuously sample water without chemical additives facilitates the use of this device in drinking water distribution systems. This work constitutes the first step in our development of a continuous, microbial capture and concentration system from large volumes of potable water. Advisors/Committee Members: Beskok, Ali (advisor), Banerjee, Debjyoti (committee member), Ley, Obdulia (committee member), Pillai, Suresh D. (committee member).

Subjects/Keywords: Pathogens; Concentration; Microfluidic; Capture; Electrophoresis

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APA (6^th Edition):

Balasubramanian, A. K. (2009). A microfluidic device for continuous capture and concentration of pathogens from water. (Thesis). Texas A&M University. Retrieved from http://hdl.handle.net/1969.1/ETD-TAMU-1339

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Balasubramanian, Ashwin Kumar. “A microfluidic device for continuous capture and concentration of pathogens from water.” 2009. Thesis, Texas A&M University. Accessed February 25, 2020. http://hdl.handle.net/1969.1/ETD-TAMU-1339.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Balasubramanian, Ashwin Kumar. “A microfluidic device for continuous capture and concentration of pathogens from water.” 2009. Web. 25 Feb 2020.

Vancouver:

Balasubramanian AK. A microfluidic device for continuous capture and concentration of pathogens from water. [Internet] [Thesis]. Texas A&M University; 2009. [cited 2020 Feb 25]. Available from: http://hdl.handle.net/1969.1/ETD-TAMU-1339.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Balasubramanian AK. A microfluidic device for continuous capture and concentration of pathogens from water. [Thesis]. Texas A&M University; 2009. Available from: http://hdl.handle.net/1969.1/ETD-TAMU-1339

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Texas A&M University

21. Rivas Cardona, Juan Alberto. Development of a microfluidic device for patterning multiple species by scanning probe lithography.

Degree: 2009, Texas A&M University

URL: http://hdl.handle.net/1969.1/ETD-TAMU-1823

► Scanning Probe Lithography (SPL) is a versatile nanofabrication platform that leverages microfluidic ?ink? delivery systems with Scanning Probe Microscopy (SPM) for generating surface-patterned chemical functionality… (more)

▼ Scanning Probe Lithography (SPL) is a versatile nanofabrication platform that leverages microfluidic ?ink? delivery systems with Scanning Probe Microscopy (SPM) for generating surface-patterned chemical functionality on the sub-100 nm length scale. One of the prolific SPL techniques is Dip Pen Nanolithography? (DPN?). High resolution, multiplexed registration and parallel direct-write capabilities make DPN (and other SPL techniques) a power tool for applications that are envisioned in micro/nano-electronics, molecular electronics, catalysis, cryptography (brand protection), combinatorial synthesis (nano-materials discovery and characterization), biological recognition, genomics, and proteomics. One of the greatest challenges for the successful performance of the DPN process is the delivery of multiple inks to the scanning probe tips for nano-patterning. The purpose of the present work is to fabricate a microfluidic ink delivery device (called ?Centiwell?) for DPN (and other SPL) applications. The device described in this study maximizes the number of chemical species (inks) for nanofabrication that can be patterned simultaneously by DPN to conform the industrial standards for fluid handling for biochemical assays (e.g., genomic and proteomic). Alternate applications of Centiwell are also feasible for the various envisioned applications of DPN (and other SPL techniques) that were listed above. The Centiwell consists of a two-dimensional array of 96 microwells that are bulk micromachined on a silicon substrate. A thermoelectric module is attached to the back side of the silicon substrate and is used to cool the silicon substrate to temperatures below the dew point. By reducing the temperature of the substrate to below the dew point, water droplets are condensed in the microwell array. Microbeads of a hygroscopic material (e.g., poly-ethylene glycol) are dispensed into the microwells to prevent evaporation of the condensed water. Furthermore, since poly-ethylene glycol (PEG) is water soluble, it forms a solution inside the microwells which is subsequently used as the ink for the DPN process. The delivery of the ink to the scanning probe tip is performed by dipping the tip (or multiple tips in an array) into the microwells containing the PEG solution. This thesis describes the various development steps for the Centiwell. These steps include the mask design, the bulk micromachining processes explored for the micro-fabrication of the microwell array, the thermal design calculations performed for the selection of the commercially available thermoelectric coolers, the techniques explored for the synthesis of the PEG microbeads, and the assembly of all the components for integration into a functional Centiwell. Finally, the successful implementation of the Centiwell for nanolithography of PEG solutions is also demonstrated. Advisors/Committee Members: Banerjee, Debjyoti (advisor), Beskok, Ali (committee member), Ley, Obdulia (committee member), Seminario, Jorge (committee member).

Subjects/Keywords: microfluidic device; Dip-Pen Nanolithography

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APA (6^th Edition):

Rivas Cardona, J. A. (2009). Development of a microfluidic device for patterning multiple species by scanning probe lithography. (Thesis). Texas A&M University. Retrieved from http://hdl.handle.net/1969.1/ETD-TAMU-1823

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Rivas Cardona, Juan Alberto. “Development of a microfluidic device for patterning multiple species by scanning probe lithography.” 2009. Thesis, Texas A&M University. Accessed February 25, 2020. http://hdl.handle.net/1969.1/ETD-TAMU-1823.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Rivas Cardona, Juan Alberto. “Development of a microfluidic device for patterning multiple species by scanning probe lithography.” 2009. Web. 25 Feb 2020.

Vancouver:

Rivas Cardona JA. Development of a microfluidic device for patterning multiple species by scanning probe lithography. [Internet] [Thesis]. Texas A&M University; 2009. [cited 2020 Feb 25]. Available from: http://hdl.handle.net/1969.1/ETD-TAMU-1823.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Rivas Cardona JA. Development of a microfluidic device for patterning multiple species by scanning probe lithography. [Thesis]. Texas A&M University; 2009. Available from: http://hdl.handle.net/1969.1/ETD-TAMU-1823

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Texas A&M University

22. Englert, Derek Lynn. Microfluidic Systems for Investigating Bacterial Chemotaxis and Colonization.

Degree: 2011, Texas A&M University

URL: http://hdl.handle.net/1969.1/ETD-TAMU-2009-12-7295

► The overall goal of this work was to develop and utilize microfluidic models for investigating bacterial chemotaxis and biofilm formation - phenotypes that play key… (more)

▼ The overall goal of this work was to develop and utilize microfluidic models for investigating bacterial chemotaxis and biofilm formation - phenotypes that play key roles in bacterial infections. Classical methods for investigating chemotaxis and biofilm formation have many limitations and drawbacks. These include being unsuitable for investigating the effect of chemorepellents, non-quantitative readouts, and not accounting for interaction between hydrodynamics and biofilm formation. The novel microfluidic model systems for chemotaxis and biofilm formation developed in this study addresses these drawbacks. Chemotaxis model system development was done in three stages. We first developed two static chemotaxis model systems - the two fluorophore chemotaxis agarose plug assay and the mu Plug assay - for rapidly determining the extent of chemotaxis in a qualitative manner. A key feature of these model systems was the incorporation of dead cells and differential labeling with green and red fluorescent proteins for partitioning the effects of movement due to fluid flow from chemotaxis. The static systems were used to rapidly screen a wide range of conditions for use in the flow-based mu Flow chemotaxis model system. The effect of four major variables - cell preparation method, gradient strength, flow rate in the device, and imaging position - that influence the chemotactic response in the mu Flow was characterized using the repellent taxis from Ni² gradients as the model chemoeffector. Using the mu Flow chemotaxis device, we investigated the chemotaxis of Escherichia coli RP437 to different signals that are present in the human gastrointestinal tract and are likely to be mediators of infection through their effect on chemotaxis. Our data show that the bacterial signal indole is a repellent, while the signals autoinducer-2 (AI-2) and isatin are attractants for E. coli RP437. However, cells exposed to a competing gradient of indole and either AI-2 or isatin, attracts E. coli. The ?Flow device was also used to refute a long-standing view on how the repellent Ni2 is sensed in E. coli. Our data show that only the Tar chemoreceptor is needed for sensing Ni² and the nickel binding protein, NikA, and the Ni² transport system proteins, NikB and NikC, are not required for repellent taxis from nickel. A microfluidic biofilm model was also developed in this study and used in conjunction with a mathematical model to investigate biofilm formation and quorum sensing in closed systems (where biofilm growth and hydrodynamics are interdependent). The mathematical model predictions were experimentally validated using Pseudomonas aeruginosa PA14 in a microfluidic biofilm system at various flow rates. Advisors/Committee Members: Jayaraman, Arul (advisor), Pillai, Suresh D. (committee member), Ugaz, Victor M. (committee member), Wood, Thomas K. (committee member).

Subjects/Keywords: chemotaxis; microfluidic; bacteria; nickel taxis

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APA (6^th Edition):

Englert, D. L. (2011). Microfluidic Systems for Investigating Bacterial Chemotaxis and Colonization. (Thesis). Texas A&M University. Retrieved from http://hdl.handle.net/1969.1/ETD-TAMU-2009-12-7295

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Englert, Derek Lynn. “Microfluidic Systems for Investigating Bacterial Chemotaxis and Colonization.” 2011. Thesis, Texas A&M University. Accessed February 25, 2020. http://hdl.handle.net/1969.1/ETD-TAMU-2009-12-7295.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Englert, Derek Lynn. “Microfluidic Systems for Investigating Bacterial Chemotaxis and Colonization.” 2011. Web. 25 Feb 2020.

Vancouver:

Englert DL. Microfluidic Systems for Investigating Bacterial Chemotaxis and Colonization. [Internet] [Thesis]. Texas A&M University; 2011. [cited 2020 Feb 25]. Available from: http://hdl.handle.net/1969.1/ETD-TAMU-2009-12-7295.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Englert DL. Microfluidic Systems for Investigating Bacterial Chemotaxis and Colonization. [Thesis]. Texas A&M University; 2011. Available from: http://hdl.handle.net/1969.1/ETD-TAMU-2009-12-7295

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Oregon State University

23. Pluess, Christoph. Application of controlled thermal expansion in diffusion bonding for the high-volume microlamination of MECS devices.

Degree: MS, Industrial Engineering, 2004, Oregon State University

URL: http://hdl.handle.net/1957/30100

Subjects/Keywords: Microfluidic devices

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Pluess, C. (2004). Application of controlled thermal expansion in diffusion bonding for the high-volume microlamination of MECS devices. (Masters Thesis). Oregon State University. Retrieved from http://hdl.handle.net/1957/30100

Chicago Manual of Style (16^th Edition):

Pluess, Christoph. “Application of controlled thermal expansion in diffusion bonding for the high-volume microlamination of MECS devices.” 2004. Masters Thesis, Oregon State University. Accessed February 25, 2020. http://hdl.handle.net/1957/30100.

MLA Handbook (7^th Edition):

Pluess, Christoph. “Application of controlled thermal expansion in diffusion bonding for the high-volume microlamination of MECS devices.” 2004. Web. 25 Feb 2020.

Vancouver:

Pluess C. Application of controlled thermal expansion in diffusion bonding for the high-volume microlamination of MECS devices. [Internet] [Masters thesis]. Oregon State University; 2004. [cited 2020 Feb 25]. Available from: http://hdl.handle.net/1957/30100.

Council of Science Editors:

Pluess C. Application of controlled thermal expansion in diffusion bonding for the high-volume microlamination of MECS devices. [Masters Thesis]. Oregon State University; 2004. Available from: http://hdl.handle.net/1957/30100

University of Cincinnati

24. BANGE, ADAM F. DEVELOPMENT AND CHARACTERIZATION OF MINIATURIZED ELECTROCHEMICAL IMMUNOSENSORS.

Degree: PhD, Arts and Sciences : Chemistry, 2007, University of Cincinnati

URL: http://rave.ohiolink.edu/etdc/view?acc_num=ucin1186764947

► The objective of this research was to improve the capabilities of immunosensing devices through miniaturization and using nanoscaled materials. Collaboration between the Chemical Sensors Group… (more)

▼ The objective of this research was to improve the capabilities of immunosensing devices through miniaturization and using nanoscaled materials. Collaboration between the Chemical Sensors Group and the UC Department of Engineering was established to develop sensors and sensing systems using modern microfabrication technology. One facet of this collaboration included work to miniaturize a microbead-based sandwich immunoassay that was developed by previous group members. A procedure to fabricate poly(dimethylsiloxane) microfluidic devices was developed to control the movement of small amounts of immunoassay reagents and microbeads. The devices were then used to evaluate bead and fluid manipulation, and detection. While the electrochemical and fluorescence measurements required for an immunoassay were demonstrated, we were not able to efficiently manipulate microbeads due to their adsorption to the microchannel walls. Research was then done to improve the detection step of a miniaturized immunoassay. A microbead based sandwich immunoassay was developed using an interdigitated array (IDA) electrode with nanoscale dimensions (220 nm electrode width, 620 nm gap). The IDA was fabricated using an electron beam lithographic lift-off technique. After an antibody-assisted capture of antigen using paramagnetic microbeads, a ß-galactosidase labeled secondary antibody was used to convert p-aminophenyl galactopyranoside (PAPG) into the redox active p-aminophenol (PAP). Amperometric detection of PAP with IDA electrodes at +300 and -200 mV vs. a Ag/AgCl reference electrode was used to measure the result, detecting MS2 bacteriophage concentrations as low as 10 ng/mL. The third research project focused on making a label-free immunosensor using an array of carbon nanotubes as an electrode and electrochemical impedance spectroscopy (EIS) for detection. Highly aligned multi-walled carbon nanotubes were grown by chemical vapor deposition using a metallic catalyst, Fe/Al2O3/SiO2, on Si wafers. The nanotube towers were removed from the silicon and cast in epoxy, then polished so that one end was exposed for electrical connection and the other used as the electrode array surface. The nanotubes were functionalized electrochemically to form carboxyl groups and then chemically conjugated to antibodies. EIS was used to directly monitor the antibody-antigen binding. Advisors/Committee Members: Halsall, Dr. H (Advisor).

Subjects/Keywords: immunoassay; electrochemistry; Interdigitated array; microfluidic

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APA (6^th Edition):

BANGE, A. F. (2007). DEVELOPMENT AND CHARACTERIZATION OF MINIATURIZED ELECTROCHEMICAL IMMUNOSENSORS. (Doctoral Dissertation). University of Cincinnati. Retrieved from http://rave.ohiolink.edu/etdc/view?acc_num=ucin1186764947

Chicago Manual of Style (16^th Edition):

BANGE, ADAM F. “DEVELOPMENT AND CHARACTERIZATION OF MINIATURIZED ELECTROCHEMICAL IMMUNOSENSORS.” 2007. Doctoral Dissertation, University of Cincinnati. Accessed February 25, 2020. http://rave.ohiolink.edu/etdc/view?acc_num=ucin1186764947.

MLA Handbook (7^th Edition):

BANGE, ADAM F. “DEVELOPMENT AND CHARACTERIZATION OF MINIATURIZED ELECTROCHEMICAL IMMUNOSENSORS.” 2007. Web. 25 Feb 2020.

Vancouver:

BANGE AF. DEVELOPMENT AND CHARACTERIZATION OF MINIATURIZED ELECTROCHEMICAL IMMUNOSENSORS. [Internet] [Doctoral dissertation]. University of Cincinnati; 2007. [cited 2020 Feb 25]. Available from: http://rave.ohiolink.edu/etdc/view?acc_num=ucin1186764947.

Council of Science Editors:

BANGE AF. DEVELOPMENT AND CHARACTERIZATION OF MINIATURIZED ELECTROCHEMICAL IMMUNOSENSORS. [Doctoral Dissertation]. University of Cincinnati; 2007. Available from: http://rave.ohiolink.edu/etdc/view?acc_num=ucin1186764947

Penn State University

25. Saksena, Pulkit. Study of Condensed Phase Reactions Between Hypergolic Propellants using Microreactors.

Degree: PhD, Mechanical Engineering, 2014, Penn State University

URL: https://etda.libraries.psu.edu/catalog/22410

► The testing of hypergolic propellants has been carried out for decades and the use of hypergolic propellants at a systems level is very well understood.… (more)

▼ The testing of hypergolic propellants has been carried out for decades and the use of hypergolic propellants at a systems level is very well understood. Extensive testing has provided the design and construction of rocket engines which have been very reliable. Safety procedures have been developed for the handling and storage for hypergolic propellants, which all too often are highly toxic. But the condensed phase chemistry for these hypergolic propellants is not very well understood. The models in place for ignition and combustion do not handle the coming together of two liquid streams of these hypergols and the two-phase aspects that are expected once the reactions occur. Moreover, it is not clear what makes a fuel and oxidizer pair hypergolic in nature. The coupling of physical and chemical factors that control the ignition of hypergolic propellants makes the direct study of the transient ignition process difficult. The current study presents a novel method to study hypergolic propellants (very fast liquid reactions) using microreactors instead of conventional drop tests, modified versions thereof or impinging jet tests. Planar counterflow microreactors are used to isolate liquid-phase reactions from secondary gas phase reactions that occur later during the ignition process and thus provide valuable insight in to the pre-ignition mechanism. The microreactor fabrication, flow field characterization, reactivity results from experiments performed using a variety of fuels and nitric acid as the oxidizer and numerical simulation results of reactive flow in the microreactor are presented in this study. Particle image velocimetry measurements and numerical simulations were conducted to characterize the laminar velocity flow-field in the microreactor and strain rates at the stagnation point along the centerline of the microreactor. Temperature measurements at the stagnation zone, the exit and positions along the length of the microreactor were used as a measure for the extent of the reaction or the heat released from the reaction. For the hypergols, an increase in reactant flow (or equivalently strain rate at the stagnation point) was found to initially increase temperatures at both the stagnation zone as well as along the length of the reactor, but eventually resulted in a decrease in temperature, revealing a maxima in temperature and reactivity. The trends indicated a reaction that was initially diffusion or heat loss controlled, which transitioned towards kinetic control at higher strain (flow) rates. Numerical simulations of the reactive flow in the microreactor stagnation zone were also carried out in which the reaction zone in the microreactor was treated similar to the reaction zone occurring in a counter-flow diffusion flame. These simulations showed that the flow rates at which the peak in temperature occurred was dependent on the rate of the reaction occurring between the hypergol pairs. The numerical simulations also show that heat loss from the top and bottom surfaces can play a role on the temperature trends…

Subjects/Keywords: Hypergolic; Propellants; Combustion; Microfluidic; Microreactor

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APA (6^th Edition):

Saksena, P. (2014). Study of Condensed Phase Reactions Between Hypergolic Propellants using Microreactors. (Doctoral Dissertation). Penn State University. Retrieved from https://etda.libraries.psu.edu/catalog/22410

Chicago Manual of Style (16^th Edition):

Saksena, Pulkit. “Study of Condensed Phase Reactions Between Hypergolic Propellants using Microreactors.” 2014. Doctoral Dissertation, Penn State University. Accessed February 25, 2020. https://etda.libraries.psu.edu/catalog/22410.

MLA Handbook (7^th Edition):

Saksena, Pulkit. “Study of Condensed Phase Reactions Between Hypergolic Propellants using Microreactors.” 2014. Web. 25 Feb 2020.

Vancouver:

Saksena P. Study of Condensed Phase Reactions Between Hypergolic Propellants using Microreactors. [Internet] [Doctoral dissertation]. Penn State University; 2014. [cited 2020 Feb 25]. Available from: https://etda.libraries.psu.edu/catalog/22410.

Council of Science Editors:

Saksena P. Study of Condensed Phase Reactions Between Hypergolic Propellants using Microreactors. [Doctoral Dissertation]. Penn State University; 2014. Available from: https://etda.libraries.psu.edu/catalog/22410

University of Manchester

26. Edwards, Francine Elizabeth. Development of novel microfluidic technologies for use within the pharmaceutical industry.

Degree: PhD, 2013, University of Manchester

URL: https://www.research.manchester.ac.uk/portal/en/theses/development-of-novel-microfluidic-technologies-for-use-within-the-pharmaceutical-industry(f22e0b81-8379-45da-838f-dccb949d1899).html ; http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.607407

► The high throughput capabilities and low sample volume requirements of microfluidic technology make it an attractive prospect for the pharmaceutical industry. This thesis concerns the… (more)

▼ The high throughput capabilities and low sample volume requirements of microfluidic technology make it an attractive prospect for the pharmaceutical industry. This thesis concerns the development of microfluidic devices to investigate two important challenges to the pharmaceutical industry: to interface microchannel systems with electrospray ionisation mass spectrometry, an extensively used technique in drug discovery and development, and to investigate drug precipitation and its prevention through formulation. A microfluidic electrospray ionisation mass spectrometry interface was developed which could be placed within the source enclosure of a Waters ZQ mass spectrometer with little requirement for modification. The microfluidic interface showed a signal improvement of 38% over a capillary voltage of 4 - 4.75 kV when compared to the commercial probe which was operated at a desolvation gas flow rate of 120 L hr-1. Under typical desolvation temperatures of 350 ºC, the commercial probe outperformed the microfluidic interface which was operated at a desolvation temperature of 60 ºC, however, only an 18% improvement in signal intensity was observed for a 290 ºC increase in temperature, and there is scope to increase the operating desolvation temperature of the microfluidic interface. A novel droplet-based microfluidic light scattering detection system was developed to monitor drug precipitation of weakly basic poorly water soluble drugs. These drugs frequently exhibit poor bioavailability and variability due to precipitation in the GI tract. A pH-shift method was used to simulate gastric emptying conditions and generate a supersaturated state. Ketoconazole was used as the model drug in this study and was found to precipitate rapidly upon supersaturation. The extent of precipitation was shown to have a linear dependence on the degree of supersaturation for physiologically relevant supersaturations. This thesis also reports the first example of microfluidic screening of precipitation inhibitors. The inhibitory effect of two water soluble polymers, polyvinylpyrrolidone (PVP) and hydroxypropyl methylcellulose (HPMC) on ketoconazole precipitation was evaluated. HPMC was found to be a more potent precipitation inhibitor than PVP, with just 0.05 mM HPMC resulting in approximately a 75% decrease in ketoconazole precipitation, outperforming that of 1.7 mM PVP, which only decreased precipitation by approximately 60%. These findings corroborate results obtained from macroscale experiments employing dynamic light scattering detection. The onset time of precipitation for a range of ketoconazole supersaturations was measured using the scattered light intensity observed from the initial 22 seconds of ketoconazole precipitation. Onset times of between 0.24 – 2.45 seconds were determined for ketoconazole supersaturations of between 30 – 65.

Subjects/Keywords: 615.1; Microfluidic; Drug Precipitation

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APA (6^th Edition):

Edwards, F. E. (2013). Development of novel microfluidic technologies for use within the pharmaceutical industry. (Doctoral Dissertation). University of Manchester. Retrieved from https://www.research.manchester.ac.uk/portal/en/theses/development-of-novel-microfluidic-technologies-for-use-within-the-pharmaceutical-industry(f22e0b81-8379-45da-838f-dccb949d1899).html ; http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.607407

Chicago Manual of Style (16^th Edition):

Edwards, Francine Elizabeth. “Development of novel microfluidic technologies for use within the pharmaceutical industry.” 2013. Doctoral Dissertation, University of Manchester. Accessed February 25, 2020. https://www.research.manchester.ac.uk/portal/en/theses/development-of-novel-microfluidic-technologies-for-use-within-the-pharmaceutical-industry(f22e0b81-8379-45da-838f-dccb949d1899).html ; http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.607407.

MLA Handbook (7^th Edition):

Edwards, Francine Elizabeth. “Development of novel microfluidic technologies for use within the pharmaceutical industry.” 2013. Web. 25 Feb 2020.

Vancouver:

Edwards FE. Development of novel microfluidic technologies for use within the pharmaceutical industry. [Internet] [Doctoral dissertation]. University of Manchester; 2013. [cited 2020 Feb 25]. Available from: https://www.research.manchester.ac.uk/portal/en/theses/development-of-novel-microfluidic-technologies-for-use-within-the-pharmaceutical-industry(f22e0b81-8379-45da-838f-dccb949d1899).html ; http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.607407.

Council of Science Editors:

Edwards FE. Development of novel microfluidic technologies for use within the pharmaceutical industry. [Doctoral Dissertation]. University of Manchester; 2013. Available from: https://www.research.manchester.ac.uk/portal/en/theses/development-of-novel-microfluidic-technologies-for-use-within-the-pharmaceutical-industry(f22e0b81-8379-45da-838f-dccb949d1899).html ; http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.607407

Louisiana State University

27. Li, Yuehao. Applications of CFD Simulations on Microfluidic Systems for Nanoparticle Synthesis.

Degree: MSChE, Chemical Engineering, 2012, Louisiana State University

URL: etd-02142013-151412 ; https://digitalcommons.lsu.edu/gradschool_theses/3914

► Microfluidics has been extensively investigated as a unique platform to synthesize nanoparticles with desired properties, e.g., size and morphology. Compared to the conventional batch reactors,… (more)

▼ Microfluidics has been extensively investigated as a unique platform to synthesize nanoparticles with desired properties, e.g., size and morphology. Compared to the conventional batch reactors, wet-chemical synthesis using continuous flow microfluidics provides better control over addition of reagents, heat and mass transfer, and reproducibility. Recently, millifluidics has emerged as an alternative since it offers similar control as microfluidics. With its dimensions scaled up to millimeter size, millifluidics saves fabrication efforts and potentially paves the way for industrial applications. Good designs and manipulations of microfluidic and millifluidic devices rely on solid understanding of fluid dynamics. Fluid flow plays an important role in heat and mass transfer; thereby, it determines the quality of the synthesized nanoparticles. Computational fluid dynamics (CFD) simulations provide an effective approach to understand various effects on fluid flows without carrying out complicated experiments. The goal of this project is to utilize CFD simulations to study flow behaviors inside microfluidic and millifluidic. Residence time distribution (RTD) analysis coupled with TEM characterization was applied to investigate the effect of reagent flow rates on particle sizes distribution. Droplet-based microfluidics, as a solution to intrinsic drawbacks associated with single-phase microfluidics, depends on proper manipulation of the flow to generate steady droplet flow. The droplet / slug formation process inside a millifluidic reactor was investigated by both experiments and numerical simulations to understand the hydrodynamics of slug breaking. Geometric optimization was carried out to analyze the dependency of slug sizes on geometric dimensions. Numerical simulations were also performed to quantify the mixing efficiency inside slugs. This work provides insight to understand fluid flow inside microfluidic and millifluidic systems. It may benefit the design and operations of novel microfluidic and millifluidic systems.

Subjects/Keywords: nanoparticle synthesis; microfluidic; CFD

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Li, Y. (2012). Applications of CFD Simulations on Microfluidic Systems for Nanoparticle Synthesis. (Masters Thesis). Louisiana State University. Retrieved from etd-02142013-151412 ; https://digitalcommons.lsu.edu/gradschool_theses/3914

Chicago Manual of Style (16^th Edition):

Li, Yuehao. “Applications of CFD Simulations on Microfluidic Systems for Nanoparticle Synthesis.” 2012. Masters Thesis, Louisiana State University. Accessed February 25, 2020. etd-02142013-151412 ; https://digitalcommons.lsu.edu/gradschool_theses/3914.

MLA Handbook (7^th Edition):

Li, Yuehao. “Applications of CFD Simulations on Microfluidic Systems for Nanoparticle Synthesis.” 2012. Web. 25 Feb 2020.

Vancouver:

Li Y. Applications of CFD Simulations on Microfluidic Systems for Nanoparticle Synthesis. [Internet] [Masters thesis]. Louisiana State University; 2012. [cited 2020 Feb 25]. Available from: etd-02142013-151412 ; https://digitalcommons.lsu.edu/gradschool_theses/3914.

Council of Science Editors:

Li Y. Applications of CFD Simulations on Microfluidic Systems for Nanoparticle Synthesis. [Masters Thesis]. Louisiana State University; 2012. Available from: etd-02142013-151412 ; https://digitalcommons.lsu.edu/gradschool_theses/3914

University of Southern California

28. Basson, Meir. Measuring the temperature of stationary fluid in a microchannel using thermochromic liquid crystals.

Degree: MS, Aerospace Engineering, 2010, University of Southern California

URL: http://digitallibrary.usc.edu/cdm/compoundobject/collection/p15799coll127/id/330842/rec/3995

► Thermochromic liquid crystals (TLC’s) have a unique chemical composition that makes them reflect different wavelengths of the visible light spectrum at different temperatures, causing them… (more)

Subjects/Keywords: microfluidic; thermochromic liquid crystals

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APA (6^th Edition):

Basson, M. (2010). Measuring the temperature of stationary fluid in a microchannel using thermochromic liquid crystals. (Masters Thesis). University of Southern California. Retrieved from http://digitallibrary.usc.edu/cdm/compoundobject/collection/p15799coll127/id/330842/rec/3995

Chicago Manual of Style (16^th Edition):

Basson, Meir. “Measuring the temperature of stationary fluid in a microchannel using thermochromic liquid crystals.” 2010. Masters Thesis, University of Southern California. Accessed February 25, 2020. http://digitallibrary.usc.edu/cdm/compoundobject/collection/p15799coll127/id/330842/rec/3995.

MLA Handbook (7^th Edition):

Basson, Meir. “Measuring the temperature of stationary fluid in a microchannel using thermochromic liquid crystals.” 2010. Web. 25 Feb 2020.

Vancouver:

Basson M. Measuring the temperature of stationary fluid in a microchannel using thermochromic liquid crystals. [Internet] [Masters thesis]. University of Southern California; 2010. [cited 2020 Feb 25]. Available from: http://digitallibrary.usc.edu/cdm/compoundobject/collection/p15799coll127/id/330842/rec/3995.

Council of Science Editors:

Basson M. Measuring the temperature of stationary fluid in a microchannel using thermochromic liquid crystals. [Masters Thesis]. University of Southern California; 2010. Available from: http://digitallibrary.usc.edu/cdm/compoundobject/collection/p15799coll127/id/330842/rec/3995

Oregon State University

29. Siripoorikan, Bunchong. Flow induced mixing in high aspect ratio microchannels.

Degree: MS, Mechanical Engineering, 2003, Oregon State University

URL: http://hdl.handle.net/1957/31550

► Micro-fluid mixing is an important aspect of many of the various micro-fluidic systems used in biochemical production, biomedical industries, micro-energy systems and some electronic devices.… (more)

▼ Micro-fluid mixing is an important aspect of many of the various micro-fluidic systems used in biochemical production, biomedical industries, micro-energy systems and some electronic devices. Typically, because of size constraints and laminar flow conditions, different fluids may only have the opportunity to mix by diffusion, which is extremely rate limited. Therefore, active or highly effective passive mixing techniques are often required. In this study, two pulsed injectors are used to actively enhance mixing in a high aspect ratio microchannel (125 μm deep and 1 mm wide). The main channel has two adjacent flowing streams with 100% dye and 0% dye concentrations, respectively. Two injectors (125 μm deep and 250 μm wide) are located on separate sides of the channel, with one downstream 2 mm (equivalent to two main channel widths or eight injector widths) from the other. This results in an asymmetric mixing as the flow proceeds downstream. A dye solution is used to map local mixing throughout the channel by measuring concentration variations as a function of both space and time. The primary flow rates are varied from 0.01 to 0.20 ml/min (Reynolds numbers of 0.3 to 26.6), the injector flow rate ratios are varied from 0.125 to 2, and the pulsing frequencies are varied from 5 to 15 Hz. Images of the concentration variations within the channel are used to quantify mixing by calibrating the intensity of the image with the concentration of the dye solution. The degree of mixing (DoM) is used as a measure of quality and is defined based on the integration across the channel of the difference between the local concentration and the 50% concentration values. DoM is normalized by the 50% concentration value and subtracted from one to yield a parameter that varies from 0 (no mixing) to 1 (perfect mixing). It is shown that there is a high degree of repeatability of concentration distribution as a function of phase of the pulsing cycle. A mixing map is constructed over the range of variables tested which indicates an optimum set of flow and pulsing conditions needed to achieve maximum mixing in the main channel flow. The flow rate ratio between the injectors and main channel is found to be the most influential parameter on overall mixing. The highest DoM in the main channel was found to be 0.89. It is also noticed that improved mixing can occur at very low flow ratios under a unique set of primary flow and low frequency pulsing conditions. In general, there is an inverse relationship between primary flow rate and pulsing frequency to achieve better overall mixing. Advisors/Committee Members: Liburdy, James A. (advisor).

Subjects/Keywords: Microfluidic devices

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Siripoorikan, B. (2003). Flow induced mixing in high aspect ratio microchannels. (Masters Thesis). Oregon State University. Retrieved from http://hdl.handle.net/1957/31550

Chicago Manual of Style (16^th Edition):

Siripoorikan, Bunchong. “Flow induced mixing in high aspect ratio microchannels.” 2003. Masters Thesis, Oregon State University. Accessed February 25, 2020. http://hdl.handle.net/1957/31550.

MLA Handbook (7^th Edition):

Siripoorikan, Bunchong. “Flow induced mixing in high aspect ratio microchannels.” 2003. Web. 25 Feb 2020.

Vancouver:

Siripoorikan B. Flow induced mixing in high aspect ratio microchannels. [Internet] [Masters thesis]. Oregon State University; 2003. [cited 2020 Feb 25]. Available from: http://hdl.handle.net/1957/31550.

Council of Science Editors:

Siripoorikan B. Flow induced mixing in high aspect ratio microchannels. [Masters Thesis]. Oregon State University; 2003. Available from: http://hdl.handle.net/1957/31550

Oregon State University

30. Enfield, Kent E. Laminate mixing in microscale fractal-like merging channel networks.

Degree: MS, Mechanical Engineering, 2003, Oregon State University

URL: http://hdl.handle.net/1957/32377

► A two-dimensional model was developed to predict concentration profiles from passive, laminar mixing of concentration layers formed in a fractal-like merging channel network. Both flat… (more)

Subjects/Keywords: Microfluidic devices

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Enfield, K. E. (2003). Laminate mixing in microscale fractal-like merging channel networks. (Masters Thesis). Oregon State University. Retrieved from http://hdl.handle.net/1957/32377

Chicago Manual of Style (16^th Edition):

Enfield, Kent E. “Laminate mixing in microscale fractal-like merging channel networks.” 2003. Masters Thesis, Oregon State University. Accessed February 25, 2020. http://hdl.handle.net/1957/32377.

MLA Handbook (7^th Edition):

Enfield, Kent E. “Laminate mixing in microscale fractal-like merging channel networks.” 2003. Web. 25 Feb 2020.

Vancouver:

Enfield KE. Laminate mixing in microscale fractal-like merging channel networks. [Internet] [Masters thesis]. Oregon State University; 2003. [cited 2020 Feb 25]. Available from: http://hdl.handle.net/1957/32377.

Council of Science Editors:

Enfield KE. Laminate mixing in microscale fractal-like merging channel networks. [Masters Thesis]. Oregon State University; 2003. Available from: http://hdl.handle.net/1957/32377

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