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Oregon State University
1.
Weymann, Davis.
Properties of hydrogels for immobilization of bacteria in a commercial microfluidic dioreactor.
Degree: MS, 2017, Oregon State University
URL: http://hdl.handle.net/1957/61554
► Current technological shortcomings limit the economic viability of capturing and utilizing small sources of methane. The development of a reactor to overcome these limitations would…
(more)
▼ Current technological shortcomings limit the economic viability of capturing and utilizing small sources of methane. The development of a reactor to overcome these limitations would unlock economic opportunity and incentivize reduced methane emissions. A
microfluidic bioreactor containing immobilized methanotrophs has the potential to overcome these limitations by profitably converting small quantities of methane to more valuable liquid products.
The material used for immobilizing bacteria in a
microfluidic bioreactor must meet four criteria: biocompatibility, mechanical stability, reactor adhesion, and economic viability. This paper describes the development of a novel blend of agar and cross-linked polyvinyl alcohol (PVA) that meets these requirements. The properties of agar/PVA blend hydrogels strongly depend on the ratio and absolute concentration of the constituent polymers, and the processes by which the polymers are cross-linked. The microscopic morphology of these blend hydrogels is theorized to be two interacting and competing phases formed by agar and PVA molecules mutually interfering with cross-linking via hydrogen bonding, and separating due to spinodal decomposition. Evidence for the proposed morphology is discussed.
Blend hydrogels of 2/5% agar/PVA are particularly promising, combining the desirable properties of both agar (low swelling) and PVA (strength and adhesion). The 2/5% agar/PVA gels exhibited little swelling in water and nitrate mineral salts-based media. They also adhered to polycarbonate and stainless steel surfaces treated with ozone or oxygen plasma. Cultures of Methylomicrobium buryatense 5GB1 (5GB1) immobilized in these gels showed a reduction of metabolic activity rates, partly due to exposure to high concentrations of sulfate and phosphate during cross-linking. Shortening cross-linker exposure time from 2 hours to 30 minutes greatly improved activity rates, and immobilized cells exhibited increased activity rates over time as fresh methane was added. Based on these results, the 2/5% agar/PVA blend hydrogels are suitable for the immobilization of 5GB1 in a
microfluidic bioreactor. Further improvement of activity rates may be possible.
Preservation of 5GB1 by lyophilization was unsuccessful. Cultures preserved in solutions of 5% bovine serum albumin and 10% sucrose or trehalose maintained metabolic activity rates after freezing at -80°C, but showed no activity after lyophilization.
Advisors/Committee Members: Schilke, Karl (advisor), Jovanovic, Goran (committee member).
Subjects/Keywords: Microfluidic
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APA (6th Edition):
Weymann, D. (2017). Properties of hydrogels for immobilization of bacteria in a commercial microfluidic dioreactor. (Masters Thesis). Oregon State University. Retrieved from http://hdl.handle.net/1957/61554
Chicago Manual of Style (16th Edition):
Weymann, Davis. “Properties of hydrogels for immobilization of bacteria in a commercial microfluidic dioreactor.” 2017. Masters Thesis, Oregon State University. Accessed March 02, 2021.
http://hdl.handle.net/1957/61554.
MLA Handbook (7th Edition):
Weymann, Davis. “Properties of hydrogels for immobilization of bacteria in a commercial microfluidic dioreactor.” 2017. Web. 02 Mar 2021.
Vancouver:
Weymann D. Properties of hydrogels for immobilization of bacteria in a commercial microfluidic dioreactor. [Internet] [Masters thesis]. Oregon State University; 2017. [cited 2021 Mar 02].
Available from: http://hdl.handle.net/1957/61554.
Council of Science Editors:
Weymann D. Properties of hydrogels for immobilization of bacteria in a commercial microfluidic dioreactor. [Masters Thesis]. Oregon State University; 2017. Available from: http://hdl.handle.net/1957/61554

Texas A&M University
2.
Sobahi, Nebras MohammedKamal A.
Development of High-Throughput Microfluidic Impedance Spectroscopy Platform for Analyzing Microdroplets in Droplet Microfluidic System.
Degree: MS, Electrical Engineering, 2014, Texas A&M University
URL: http://hdl.handle.net/1969.1/153517
► This thesis presents the development of a high-throughput microfluidic impedance spectroscopy platform for electrically detecting analyzing impedance measurements of non-contact and label free microdroplets. This…
(more)
▼ This thesis presents the development of a high-throughput
microfluidic impedance spectroscopy platform for electrically detecting analyzing impedance measurements of non-contact and label free microdroplets. This
microfluidic impedance spectroscopy platform gives valuable information of the size and contents of the microdroplets in general and particularly of cells encapsulated within droplets.
Impedance spectroscopy is a common method for analyzing dielectric properties of particles with respect to the stimulating frequency.
Microfluidic based impedance spectroscopy can analyze up to micro size particles. However, droplets based
microfluidic impedance spectroscopy systems for analyzing cells encapsulated within droplets have been rarely developed.
However, to develop a high-throughput system, a novel sensitive high-throughput droplets based
microfluidic impedance spectroscopy platform for analyzing cells encapsulated with droplets at different levels concentrations at throughput of 140 Hz which has not been reported in the literature yet.
The device sensitivity was demonstrated using chlamydomonas reinhardtii cells. Two throughputs (17 and 140 droplets/s) for four level of cells concentrations were discriminating and compared. The maximum deviation in the acquired data for both cases was 6.9%. At 10% difference of cells encapsulated within droplets, the device was capable of discriminating and distinguishing different between the encapsulated microdroplets.
Advisors/Committee Members: Han, Arum (advisor), Ji, Jim X (committee member), Palermo, Samuel (committee member), Yakovlev, Vladislav V (committee member).
Subjects/Keywords: microfluidic; impedance
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APA (6th Edition):
Sobahi, N. M. A. (2014). Development of High-Throughput Microfluidic Impedance Spectroscopy Platform for Analyzing Microdroplets in Droplet Microfluidic System. (Masters Thesis). Texas A&M University. Retrieved from http://hdl.handle.net/1969.1/153517
Chicago Manual of Style (16th Edition):
Sobahi, Nebras MohammedKamal A. “Development of High-Throughput Microfluidic Impedance Spectroscopy Platform for Analyzing Microdroplets in Droplet Microfluidic System.” 2014. Masters Thesis, Texas A&M University. Accessed March 02, 2021.
http://hdl.handle.net/1969.1/153517.
MLA Handbook (7th Edition):
Sobahi, Nebras MohammedKamal A. “Development of High-Throughput Microfluidic Impedance Spectroscopy Platform for Analyzing Microdroplets in Droplet Microfluidic System.” 2014. Web. 02 Mar 2021.
Vancouver:
Sobahi NMA. Development of High-Throughput Microfluidic Impedance Spectroscopy Platform for Analyzing Microdroplets in Droplet Microfluidic System. [Internet] [Masters thesis]. Texas A&M University; 2014. [cited 2021 Mar 02].
Available from: http://hdl.handle.net/1969.1/153517.
Council of Science Editors:
Sobahi NMA. Development of High-Throughput Microfluidic Impedance Spectroscopy Platform for Analyzing Microdroplets in Droplet Microfluidic System. [Masters Thesis]. Texas A&M University; 2014. Available from: http://hdl.handle.net/1969.1/153517
3.
Thelwell, Jeray Anthony.
Carrier bead systems in the microfluidic isolation of cfDNA
for downstream analysis.
Degree: Biomedical Engineering, 2017, Brown University
URL: https://repository.library.brown.edu/studio/item/bdr:733538/
► The field of medical diagnostics is governed by two practical goals that drive the engineering and relevant innovation necessary to move the technology forward. Firstly,…
(more)
▼ The field of medical diagnostics is governed by two
practical goals that drive the engineering and relevant innovation
necessary to move the technology forward. Firstly, diagnostic
technology must have predictive potential. As medical education
continues to develop with the growing body of basic biological
research, there is increased emphasis on preventative medicine.
Instead of training more highly skilled surgical specialty doctors
to mechanically remedy exacerbated ailments of the body, lives are
now being saved before patients even make it into the operating
room. Predictive diagnostic technologies provide a wealth of
information, not only about current pathological status, but also
about the real-time effects of treatment with little to no harm of
the patient. Secondly, diagnostic technology must offer
personalized patient information. With completion of the human
genome project and the advent of more sophisticated sequencing
modalities, medical care tailored to specific individuals is more
of a possibility. A greater understanding of epigenetic modulation
of the genome is promoting the transition from “one size fits all”
care. While approaching this problem in the Tripathi Lab, two
additional constraints were imposed: the diagnostic technology must
be robust yet economical and simple to use in order to allow for
widespread adoption. To that end, a
microfluidic platform for the
extraction of short fragment DNA from crowded solution is reported
with the idea of mimicking cell-free DNA found in blood.
Preliminary off-chip experiments comparing different magnetic beads
to determine the optimal bead and buffer composition for short DNA
fragments are first completed. Then an investigation into
multi-bead systems for size based extraction through the oil-water
interface is carried out. Finally, on-chip short fragment DNA
recovery is demonstrated from buffer solution and spiked human
serum solution.
Advisors/Committee Members: Tripathi, Anubhav (Advisor), Breuer, Kenneth (Reader), Mathiowitz, Edith (Reader), Wessel, Gary (Reader).
Subjects/Keywords: Microfluidic devices
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APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Thelwell, J. A. (2017). Carrier bead systems in the microfluidic isolation of cfDNA
for downstream analysis. (Thesis). Brown University. Retrieved from https://repository.library.brown.edu/studio/item/bdr:733538/
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Thelwell, Jeray Anthony. “Carrier bead systems in the microfluidic isolation of cfDNA
for downstream analysis.” 2017. Thesis, Brown University. Accessed March 02, 2021.
https://repository.library.brown.edu/studio/item/bdr:733538/.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Thelwell, Jeray Anthony. “Carrier bead systems in the microfluidic isolation of cfDNA
for downstream analysis.” 2017. Web. 02 Mar 2021.
Vancouver:
Thelwell JA. Carrier bead systems in the microfluidic isolation of cfDNA
for downstream analysis. [Internet] [Thesis]. Brown University; 2017. [cited 2021 Mar 02].
Available from: https://repository.library.brown.edu/studio/item/bdr:733538/.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Thelwell JA. Carrier bead systems in the microfluidic isolation of cfDNA
for downstream analysis. [Thesis]. Brown University; 2017. Available from: https://repository.library.brown.edu/studio/item/bdr:733538/
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Oregon State University
4.
Rundel, Jack T.
Design, fabrication and application of a microfluidic nanofiltration module for separation and purification of macromolecules and nanoparticles.
Degree: PhD, Chemistry, 2008, Oregon State University
URL: http://hdl.handle.net/1957/7682
► A microfluidic nanofiltration module has been designed, fabricated and applied to the continuous-flow, pressure-driven, post-synthetic purification of macromolecules and nanoparticles via diafiltration using a commercially…
(more)
▼ A
microfluidic nanofiltration module has been designed, fabricated and applied to the continuous-flow, pressure-driven, post-synthetic purification of macromolecules and nanoparticles via diafiltration using a commercially available organic solvent resistant nanofiltration membrane, STARMEM 122. This module will readily interface with other microscale components within a
"nanofactory" for the rapid synthesis, purification and delivery of highly
dispersed macromolecules and nanoparticles. The
microfluidic nanofiltration
module (MNM) fully integrates the membrane into an all-polymer format
constructed of materials that are relatively inexpensive, chemically compatible
with the targeted compounds and transmissive to visible light allowing optical
monitoring of the system.
A molecular weight cutoff of 2.3 kDa was determined for the MNM using
half-generation poly(amidoamine) dendrimer standards in a simulated postsynthetic
mixture of 4 mM methyl acrylate in methanol. The membrane was
also characterized within a macroscale test fixture (MTF), constructed of
reusable parts that are easily disassembled for rapid replacement of the
membrane. Rejections of 95% to 97% were observed in the MTF for
triphenylphosphine (PPh3) stabilized gold-eleven (Au11) nanoparticle
standards. Rejections of 12% to 58% were also observed for PPh₃, an
anticipated post-synthetic byproduct. Purification via diafiltration of realworld
post-synthetic Au₁₁ mixtures was demonstrated in the MTF. Au₁₁ rejections > 99% were observed along with expected decreases in PPh₃ concentrations. Stable permeances of 2 L m⁻² h⁻¹ bar⁻¹ were also observed,
comparable to those reported in the literature and claimed by the membrane
manufacturer.
The purity of the Au₁₁ final product was comparable to that of the crystalline
Au11 standard purified by traditional means. These results suggest that a
diafiltration system incorporating an organic solvent resistant nanofiltration
membrane would be practical for rapid purification and high product recovery
of gold nanoparticles in an organic solvent environment immediately
downstream of a microreactor within a nanofactory architecture.
Advisors/Committee Members: Remcho, Vincent T (advisor), Paul, Brian K (committee member).
Subjects/Keywords: microfluidic; Microfluidics
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❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Rundel, J. T. (2008). Design, fabrication and application of a microfluidic nanofiltration module for separation and purification of macromolecules and nanoparticles. (Doctoral Dissertation). Oregon State University. Retrieved from http://hdl.handle.net/1957/7682
Chicago Manual of Style (16th Edition):
Rundel, Jack T. “Design, fabrication and application of a microfluidic nanofiltration module for separation and purification of macromolecules and nanoparticles.” 2008. Doctoral Dissertation, Oregon State University. Accessed March 02, 2021.
http://hdl.handle.net/1957/7682.
MLA Handbook (7th Edition):
Rundel, Jack T. “Design, fabrication and application of a microfluidic nanofiltration module for separation and purification of macromolecules and nanoparticles.” 2008. Web. 02 Mar 2021.
Vancouver:
Rundel JT. Design, fabrication and application of a microfluidic nanofiltration module for separation and purification of macromolecules and nanoparticles. [Internet] [Doctoral dissertation]. Oregon State University; 2008. [cited 2021 Mar 02].
Available from: http://hdl.handle.net/1957/7682.
Council of Science Editors:
Rundel JT. Design, fabrication and application of a microfluidic nanofiltration module for separation and purification of macromolecules and nanoparticles. [Doctoral Dissertation]. Oregon State University; 2008. Available from: http://hdl.handle.net/1957/7682

University of KwaZulu-Natal
5.
Moodley, Revesa Sadasivan.
Evaluation of paper substrates for microfluidic application in medical diagnostic kits.
Degree: 2018, University of KwaZulu-Natal
URL: https://researchspace.ukzn.ac.za/handle/10413/16613
► Recent studies in the biomedical field have shown the use of paper in microfluidic analytical devices. However, no studies have been undertaken to ascertain what…
(more)
▼ Recent studies in the biomedical field have shown the use of paper in
microfluidic analytical devices. However, no studies have been undertaken to ascertain what type(s) of paper substrates are ideal for such
microfluidic applications. Hence, this study was conducted to determine the feasibility of using different paper substrates for implementation in
microfluidic analytical devices, specifically in the South African context, compared to currently used materials such as glass and silicone. In addition, fibres in paper substrates were substituted with nanofibrillated cellulose (NFC) fibres to ascertain the impact of NFC on the microfluidics of the paper substrates. The wicking rate of the substrates was the focus of the study, with high -resolution field emission gun-scanning electron microscopy and contact angle tests being used to support the results obtained. Solid wax printing was also conducted to determine whether the paper substrates were suited to fabrication for
microfluidic applications.
High-resolution morphological studies of the paper substrates showed that pore sizes of the pulp fibres were in the order sulphite> bleached kraft > unbleached kraft > Whatman No. 1 Chromatography Filter Paper > Whatman 3MM Chromatography Filter Paper > thermo-mechanical > recycled. It was concluded that the larger pore size of fibres correlated with faster wicking rates of the relevant paper substrates. The substitution of pulp fibres with NFC led to reduced pore size of the fibres thus leading to reduced wicking rates due to the presence of the small NFC particles.
Contact angle is directly linked to the hydrophobicity of the substrate and is indicative of the resistance to absorption of a liquid. The results revealed that all the paper substrates were hydrophilic. However, the hydrophilicity of two of the substrates (sheets substituted with 100% NFC unbleached kraft pulp and 100% NFC recycled pulp) were higher than those of the other substrates indicating that, although these substrates were still hydrophilic in nature, their absorption of aqueous liquid would take longer periods of time.
The results showed that Whatman glass microfiber GF/D filter paper was the fastest wicking substrate, using both dye and blood simulant as wicking liquids. Similarly, paper substrates made with recycled fibres exhibited the slowest wicking rates when using both wicking liquids. These results can be used when determining which of the substrates to use for paper-based
microfluidic device (μPAD) application, whereby the desired detection time would be the factor used to establish which of the substrates to use. Comparison of vertical and horizontal tests showed varying results. In theory, the horizontal wicking test should result in a faster wicking rate than the vertical test, taking hydrostatic pressure into consideration. The majority of the substrates showed that the horizontal wicking rate was faster when using the dye solution, whereas vertical wicking was faster when using the blood simulant. Discrepancies between the results…
Advisors/Committee Members: Land, Kevin. (advisor), Sithole, Bishop Bruce. (advisor), Van Zyl, Werner Ewald. (advisor), Andrew, Jerome. (advisor).
Subjects/Keywords: MICROFLUIDIC.; DIAGNOSTIC.
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❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Moodley, R. S. (2018). Evaluation of paper substrates for microfluidic application in medical diagnostic kits. (Thesis). University of KwaZulu-Natal. Retrieved from https://researchspace.ukzn.ac.za/handle/10413/16613
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Moodley, Revesa Sadasivan. “Evaluation of paper substrates for microfluidic application in medical diagnostic kits.” 2018. Thesis, University of KwaZulu-Natal. Accessed March 02, 2021.
https://researchspace.ukzn.ac.za/handle/10413/16613.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Moodley, Revesa Sadasivan. “Evaluation of paper substrates for microfluidic application in medical diagnostic kits.” 2018. Web. 02 Mar 2021.
Vancouver:
Moodley RS. Evaluation of paper substrates for microfluidic application in medical diagnostic kits. [Internet] [Thesis]. University of KwaZulu-Natal; 2018. [cited 2021 Mar 02].
Available from: https://researchspace.ukzn.ac.za/handle/10413/16613.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Moodley RS. Evaluation of paper substrates for microfluidic application in medical diagnostic kits. [Thesis]. University of KwaZulu-Natal; 2018. Available from: https://researchspace.ukzn.ac.za/handle/10413/16613
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Rutgers University
6.
Prakash, Shreya, 1994-.
A microfluidic biosensing architecture for multiplexed analyte detection using hydrogel barcoded particles.
Degree: MS, Electrical and Computer Engineering, 2019, Rutgers University
URL: https://rucore.libraries.rutgers.edu/rutgers-lib/61911/
► Multiplexing is a method of analyzing multiple analytes in a biological assay in a single step. It provides advantage of shorter processing time, low sample…
(more)
▼ Multiplexing is a method of analyzing multiple analytes in a biological assay in a single step. It provides advantage of shorter processing time, low sample volume and reduced cost per test. Currently, Flow Cytometers are used for blood cells enumeration, however, they are expensive, requires fluorescent tagging of cells and trained technicians to operate the instrument. We propose a non-fluorescent
microfluidic architecture with single excitation and detection scheme using the barcoded particles fabricated using Stop Flow Lithography process. The barcoded particles designed with specific number of coding regions can generate numerous distinct patterns of electrical signatures and can be used in biological assays to detect multiple blood cells.
A novel design of the asymmetric rectangular barcoded particle was proposed and tested in COMSOL Multiphysics. The barcoded particle with five coding regions generated distinct bi-polar signatures in the
microfluidic impedance detection system. Different configurations of the sensing system were proposed to detect the conjugated microspheres (representative of blood cells) effectively based on the site of conjugation. The bottom co-planar electrode and top-bottom electrode configurations were tested for sensitive detection of conjugated microspheres to the barcoded particle. We found that our
microfluidic detection system was sensitive enough to detect the presence of a microsphere attached to the barcoded particle. Further, we also investigated the effect of microspheres conjugation orientation to the barcoded particle and their associated electrical signatures. We developed a multi-feature selection algorithm to solve the orientation problem and improved the accuracy of our sensing mechanism. Finally, we fabricated the
microfluidic chips using lithography, and co-planar electrodes on glass surfaces using thin film deposition process in the clean room. Our proposed
microfluidic system can enumerate multiple blood cells in a single assay using barcoded particles. The concentration of these cells provides useful information about disease onset and progression. Such sensors can be used for diagnostic and management of diseases like Sepsis, Acute Kidney Injury, HIV/ AIDS.
Advisors/Committee Members: Hassan, Umer (chair), Gajic, Zoran (internal member), Wu, Chung-Tse Michael (internal member), School of Graduate Studies.
Subjects/Keywords: Microfluidic; Biosensors
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❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Prakash, Shreya, 1. (2019). A microfluidic biosensing architecture for multiplexed analyte detection using hydrogel barcoded particles. (Masters Thesis). Rutgers University. Retrieved from https://rucore.libraries.rutgers.edu/rutgers-lib/61911/
Chicago Manual of Style (16th Edition):
Prakash, Shreya, 1994-. “A microfluidic biosensing architecture for multiplexed analyte detection using hydrogel barcoded particles.” 2019. Masters Thesis, Rutgers University. Accessed March 02, 2021.
https://rucore.libraries.rutgers.edu/rutgers-lib/61911/.
MLA Handbook (7th Edition):
Prakash, Shreya, 1994-. “A microfluidic biosensing architecture for multiplexed analyte detection using hydrogel barcoded particles.” 2019. Web. 02 Mar 2021.
Vancouver:
Prakash, Shreya 1. A microfluidic biosensing architecture for multiplexed analyte detection using hydrogel barcoded particles. [Internet] [Masters thesis]. Rutgers University; 2019. [cited 2021 Mar 02].
Available from: https://rucore.libraries.rutgers.edu/rutgers-lib/61911/.
Council of Science Editors:
Prakash, Shreya 1. A microfluidic biosensing architecture for multiplexed analyte detection using hydrogel barcoded particles. [Masters Thesis]. Rutgers University; 2019. Available from: https://rucore.libraries.rutgers.edu/rutgers-lib/61911/

Oregon State University
7.
Koesdjojo, Myra T.
Fabrication and application of polymer based microfluidic devices.
Degree: PhD, Chemistry, 2009, Oregon State University
URL: http://hdl.handle.net/1957/10841
► The concept of reducing laboratory operations in scale such that they fit on a microfluidic chip has been met with great enthusiasm. Lab-on-a-chip devices promise…
(more)
▼ The concept of reducing laboratory operations in scale such that they fit on
a
microfluidic chip has been met with great enthusiasm. Lab-on-a-chip devices
promise to be cost effective to operate due to reduced reagent consumption, have
the potential to offer shorter analysis times due to their short path lengths, and may
be useful in biological applications in that they are inherently compact and
inexpensive to build, thus they may be disposable. In this work, a series of
fabrication techniques for the production of polymer-based
microfluidic devices
are explored.
In the first component of this research effort an aluminum mold was
fabricated using CNC machining to create the desired microchannel design,
followed by a two-stage embossing process, involving two polymer substrates
with different glass transition temperatures (Tg), polyetherimide (PEI; Tg~216 °C)
and poly(methyl methacrylate) (PMMA; Tg~105 °C). Successful feature transfer
from aluminum mold to PMMA substrates was achieved reproducibly employing
this method. With this approach, the expensive process of producing the aluminum
master need be performed only once. Electrophoretic separations of fluorescent
dyes, rhodamine B and fluorescein were performed on the PMMA microchips,
with peak efficiencies of 55500 and 66300 theoretical plates/meter, respectively.
The next stage of work explored a new bonding method by solvent welding
using ice as sacrificial layer to prevent channel deformation. Water is one of the
most compatible sacrificial media; it is readily available, non toxic, has a low
evaporation rate, a high freezing point relative to the bonding solvent, and a low
melting point which makes it easier to flush out after sealing, as compared to using
other sacrificial media (paraffin wax or low-melting temperature alloys). The
bonded PMMA microchips could withstand an internal pressure of > 2000 psi,
more than 17 times stronger than the thermally bonded chips.
In the final stage of work a new bonding technique was developed that
readily produces complete
microfluidic chips, without the need of a sacrificial
layer to form complete multilayer
microfluidic devices. Also developed was the
use of an SU-8 master in the two-stage embossing process to create microchannels.
This approach is faster, simpler and less costly than CNC machining. The
fabrication technique was utilized to build a
microfluidic liquid chromatography
(LC) system that was shown to generate high separation efficiencies of 10,000-
45,000 plates/m. In addition, a passive micromixer containing high-density
microfeatures was fabricated to perform a glycine assay using O-phthaldialdehyde.
With glycine concentrations ranging from 0.0 to 2.6 μM, a linear calibration plot
(R2 = 0.9982) was obtained with a detection limit of 0.032 μM.
Advisors/Committee Members: Remcho, Vincent (advisor), Chang, Chih-hung (committee member).
Subjects/Keywords: microfluidic; Microfluidic devices – Design and construction
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❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Koesdjojo, M. T. (2009). Fabrication and application of polymer based microfluidic devices. (Doctoral Dissertation). Oregon State University. Retrieved from http://hdl.handle.net/1957/10841
Chicago Manual of Style (16th Edition):
Koesdjojo, Myra T. “Fabrication and application of polymer based microfluidic devices.” 2009. Doctoral Dissertation, Oregon State University. Accessed March 02, 2021.
http://hdl.handle.net/1957/10841.
MLA Handbook (7th Edition):
Koesdjojo, Myra T. “Fabrication and application of polymer based microfluidic devices.” 2009. Web. 02 Mar 2021.
Vancouver:
Koesdjojo MT. Fabrication and application of polymer based microfluidic devices. [Internet] [Doctoral dissertation]. Oregon State University; 2009. [cited 2021 Mar 02].
Available from: http://hdl.handle.net/1957/10841.
Council of Science Editors:
Koesdjojo MT. Fabrication and application of polymer based microfluidic devices. [Doctoral Dissertation]. Oregon State University; 2009. Available from: http://hdl.handle.net/1957/10841

Cornell University
8.
Santana, Steven.
Microfluidic Analysis Of Metastatic Cancer Biomarkers.
Degree: PhD, Mechanical Engineering, 2014, Cornell University
URL: http://hdl.handle.net/1813/38925
► Cancer is the second-leading cause of death in the United States. Metastasis is responsible for 90% of cancer-related death and progresses through multifarious, poorly-understood cascades…
(more)
▼ Cancer is the second-leading cause of death in the United States. Metastasis is responsible for 90% of cancer-related death and progresses through multifarious, poorly-understood cascades as they are difficult to observe in vivo. It is widely held that deciphering the metastatic cascade and identifying metastatic precursors will lead to improved patient outcomes. In this work, we isolate and study cancer biomarkers, specifically circulating tumor cells (CTCs) and cancer-cell-derived extracellular shed vesicles (ESVs), implicated in cancer progression and metastasis. First we describe the design, fabrication, and use of a Hele-Shaw
microfluidic system to optimize rarecell immunocapture parameters with a focus on informing the design of systems for CTC isolation from patient whole blood. Our study includes the role of antibody selection, density, antigen locations, and multi-modal capture surfaces, as well as shear stress, on rare cell capture. We use LNCaPs, a PSMA-expressing prostate cancer cell line, as a model for CTCs and anti-PSMA antibodies, J591 and J415, to inform chemistry-mediated immobilization. Next, we focus on another cancer-disseminated marker, extracellular shed vesicles. ESVs, including exosomes and cancer-cell-derived microvesicles, are disseminated throughout the body and represent an important conduit of cell communication. Microvesicles have potential as a cancer biomarker as they are believed to transform tumor microenvironments and prime the metastatic niche. Cancercell-derived ESV subpopulations consist of a small-diameter exosome population and a large-diameter, cancer-cell-specific microvesicle population, each formed by unique mechanisms. It is believed that size correlates with biological properties of interest, but isolating these subpopulations, to discern chemical, biological, or physical differences, is challenging. We designed a deterministic lateral displacement
microfluidic platform to isolate a pure microvesicle sample from the heterogeneous cancer-cell-derived ESV population. The threshold diameter differentiating the microvesicle population from the exosome population was determined by characterizing the size distributions of ESVs harvested from multiple cancer cell lines of breast, brain, and pancreas origin. Our microvesicle-isolation
microfluidic technology facilitates future investigations regarding microvesicles' role in cancer progression by enabling identification of cargo carried by the microvesicle subpopulation.
Advisors/Committee Members: Kirby, Brian (chair), Fischbach, Claudia (committee member), Sipple, John W (committee member), Cerione, Richard A (committee member).
Subjects/Keywords: microfluidic; metastasis; biomarker
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MLA ·
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APA (6th Edition):
Santana, S. (2014). Microfluidic Analysis Of Metastatic Cancer Biomarkers. (Doctoral Dissertation). Cornell University. Retrieved from http://hdl.handle.net/1813/38925
Chicago Manual of Style (16th Edition):
Santana, Steven. “Microfluidic Analysis Of Metastatic Cancer Biomarkers.” 2014. Doctoral Dissertation, Cornell University. Accessed March 02, 2021.
http://hdl.handle.net/1813/38925.
MLA Handbook (7th Edition):
Santana, Steven. “Microfluidic Analysis Of Metastatic Cancer Biomarkers.” 2014. Web. 02 Mar 2021.
Vancouver:
Santana S. Microfluidic Analysis Of Metastatic Cancer Biomarkers. [Internet] [Doctoral dissertation]. Cornell University; 2014. [cited 2021 Mar 02].
Available from: http://hdl.handle.net/1813/38925.
Council of Science Editors:
Santana S. Microfluidic Analysis Of Metastatic Cancer Biomarkers. [Doctoral Dissertation]. Cornell University; 2014. Available from: http://hdl.handle.net/1813/38925

University of Wisconsin – Milwaukee
9.
Amin, Alaknanda.
Microfluidic Device Integration of Electrostatic Corral Trapping Systems.
Degree: MS, Chemistry, 2014, University of Wisconsin – Milwaukee
URL: https://dc.uwm.edu/etd/519
► This thesis describes the development, characterization, and application of the microfluidics device integration of electrostatic corral trapping systems. Optical traps or "laser tweezers", which…
(more)
▼ This thesis describes the development, characterization, and application of the microfluidics device integration of electrostatic corral trapping systems. Optical traps or "laser tweezers", which are capable of trapping microscopic dielectric particles through the production of steep electromagnetic field gradients, have been significant in the development of the field of biophysics and the manipulation of microscopic objects. This method of trapping unfortunately has a fundamental size limitation, making it incapable of trapping molecular-scale objects. We have developed a new tool for the trapping and manipulation of nanoscale objects including single molecules, the corral trap, which has distinct characteristics that set it apart from other trapping techniques. In order to increase the versatility of this new trapping tool, steps have been taken to integrate corral traps in a
microfluidic cell. The production of such integrated devices based on optical lithography techniques will be presented in detail. Corral trapping in microfluidics device is expected to have important future applications in areas such as biomedical assays, ultra-sensitive biochemical analysis, and DNA manipulation and screening. Novelty: A novel method for the trapping of single molecules has been successfully used for the trapping of single ssDNA molecules.
Advisors/Committee Members: Jörg Woehl.
Subjects/Keywords: Microfluidic; Trapping; Chemistry
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❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Amin, A. (2014). Microfluidic Device Integration of Electrostatic Corral Trapping Systems. (Thesis). University of Wisconsin – Milwaukee. Retrieved from https://dc.uwm.edu/etd/519
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Amin, Alaknanda. “Microfluidic Device Integration of Electrostatic Corral Trapping Systems.” 2014. Thesis, University of Wisconsin – Milwaukee. Accessed March 02, 2021.
https://dc.uwm.edu/etd/519.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Amin, Alaknanda. “Microfluidic Device Integration of Electrostatic Corral Trapping Systems.” 2014. Web. 02 Mar 2021.
Vancouver:
Amin A. Microfluidic Device Integration of Electrostatic Corral Trapping Systems. [Internet] [Thesis]. University of Wisconsin – Milwaukee; 2014. [cited 2021 Mar 02].
Available from: https://dc.uwm.edu/etd/519.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Amin A. Microfluidic Device Integration of Electrostatic Corral Trapping Systems. [Thesis]. University of Wisconsin – Milwaukee; 2014. Available from: https://dc.uwm.edu/etd/519
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Oregon State University
10.
Tennico, Yolanda H.
Magnetic particles for selective extraction of trace analytes in microfluidic devices.
Degree: PhD, Chemistry, 2010, Oregon State University
URL: http://hdl.handle.net/1957/14783
► The development of micro total analysis systems (µTAS), also called “lab-on-a-chip”, or microfluidic analysis systems, is presented in this dissertation. Various research areas, covering subjects…
(more)
▼ The development of micro total analysis systems (µTAS), also called “lab-on-a-chip”, or
microfluidic analysis systems, is presented in this dissertation. Various research areas, covering subjects from magnetic particles synthesis to novel microchip fabrication techniques, are explored to develop a lab-on-a-chip system capable of performing magnetic bead-based bioassays. These devices are proven not only to be cost-effective (due to their reduced reagent consumption), but also time-efficient (shorter analysis time), and may be disposable (they are inherently compact and inexpensive to produce), making them very attractive to be used in biomedical applications.
The research starts with the utilization of functionalized magnetic particles as sorbents for an in-line extraction in confines of capillary columns. The synthesized silica-coated iron oxide particles functionalized with C18 groups were used as reversed-phase sorbents. Magnets were used to locally immobilize these particles inside the capillary. The results showed that extraction, elution, and detection of the analytes were performed sequentially without interruption or need for sample handling.
The work continued with the development of bonding techniques for creating polymer-based
microfluidic devices through surface modification process. This technique readily produced complete
microfluidic chips via plasma oxidation followed by silane reagent treatment (tetraethyl orthosilicate) to facilitate siloxane bonds between the two polymers. It provides a versatile approach to bond dissimilar polymer substrates and between a hard polymer substrate to polydimethylsiloxane or glass.
The two previous studies were combined allowing for the integration of magnetic particles in a
microfluidic chip format. The benefits of each component were utilized to develop an on-chip aptamer-based fluorescence assay for thrombin detection and quantification based on sandwich ELISA principles. Aptamer-functionalized magnetic beads were utilized to capture the target analyte, while a second aptamer, functionalized with quantum dots, was employed for on-chip detection. Disposable
microfluidic devices were employed as the platform to enable rapid and sensitive thrombin detection with the benefits of reduced costs, minimized material consumption, and simplified washing process. This work has opened up a new chapter in the development of magnetic beads-based materials and devices in a wide variety of applications for detection of target proteins of biomedical importance.
Advisors/Committee Members: Remcho, Vincent T. (advisor), Lerner, Michael (committee member).
Subjects/Keywords: microfluidics; Microfluidic devices
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❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Tennico, Y. H. (2010). Magnetic particles for selective extraction of trace analytes in microfluidic devices. (Doctoral Dissertation). Oregon State University. Retrieved from http://hdl.handle.net/1957/14783
Chicago Manual of Style (16th Edition):
Tennico, Yolanda H. “Magnetic particles for selective extraction of trace analytes in microfluidic devices.” 2010. Doctoral Dissertation, Oregon State University. Accessed March 02, 2021.
http://hdl.handle.net/1957/14783.
MLA Handbook (7th Edition):
Tennico, Yolanda H. “Magnetic particles for selective extraction of trace analytes in microfluidic devices.” 2010. Web. 02 Mar 2021.
Vancouver:
Tennico YH. Magnetic particles for selective extraction of trace analytes in microfluidic devices. [Internet] [Doctoral dissertation]. Oregon State University; 2010. [cited 2021 Mar 02].
Available from: http://hdl.handle.net/1957/14783.
Council of Science Editors:
Tennico YH. Magnetic particles for selective extraction of trace analytes in microfluidic devices. [Doctoral Dissertation]. Oregon State University; 2010. Available from: http://hdl.handle.net/1957/14783

Oregon State University
11.
Nammoonnoy, Jintana.
A photoactivable microfluidic device for heavy metal ion extraction.
Degree: PhD, Chemistry, 2010, Oregon State University
URL: http://hdl.handle.net/1957/18461
► The development of microfluidic devices for heavy metal extraction is presented in this dissertation. Various research areas, covering subjects from photochromic compound syntheses to microchip…
(more)
▼ The development of
microfluidic devices for heavy metal extraction is presented in this dissertation. Various research areas, covering subjects from photochromic compound syntheses to microchip fabrication techniques are explored to develop
microfluidic devices capable of extracting heavy metal ions from drinking water. Through integration of the beneficial characteristics of both
microfluidic devices and photochromic dyes, a simple and innovative microchip configured as a photoactivable extraction system for metal ion accumulation and release can be realized.
The initial research focused on the utilization of photochromic compounds namely spiropyrans, as chelators for heavy metal extraction. Spiropyrans are organic photochromic compounds that have been widely studied. Upon irradiation with UV or visible light, spiropyrans isomerize between the closed and open forms, in which the open form is comparatively more polar. Metal ions can influence this isomerization process by associating with the open form through the electron-rich oxygen atom. In contrast, visible light produces a high concentration of the closed form, and thus hinders metal-binding. Spiropyrans, therefore, show great potential as photo-reversible metal-complexation agents. The spiropyran derivatives were synthesized and immobilized on solid supports including polymeric resins and poly(methylmethacrylate) (PMMA) microchips. Metal ion uptake can be triggered using UV light and subsequently reversed on demand by shining green light on the colored complex, which regenerates the inactive spiropyran form resulting in the release of metal ions. The use of light to trigger the chelator offers unique opportunities.
The work continued with the development of
microfluidic fabrication for creating versatile, solvent-compatible
microfluidic devices through surface modification. There are challenges associated with the use of polymeric based
microfluidic devices, particularly in surface modification steps, as solvents can embrittle thermoplastics that resulting in microcracking. To overcome this limitation, we explored the possibility of fabricating extremely robust
microfluidic devices entirely from fluorocarbon and glass materials. The work demonstrated a new fabrication technique based on a chemically activated poly(tetrafluoro ethylene) (PTFE) sheet sandwiched between chemically activated glass substrates. The PTFE microchannels can be fabricated in minutes using a cutting plotter to create microchannels. The possibility of glass to PTFE makes this method applicable in a wide range of applications.
Advisors/Committee Members: Remcho, Vincent T. (advisor), Walker, Jeffrey R. (committee member).
Subjects/Keywords: Microfluidics; Microfluidic devices
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❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Nammoonnoy, J. (2010). A photoactivable microfluidic device for heavy metal ion extraction. (Doctoral Dissertation). Oregon State University. Retrieved from http://hdl.handle.net/1957/18461
Chicago Manual of Style (16th Edition):
Nammoonnoy, Jintana. “A photoactivable microfluidic device for heavy metal ion extraction.” 2010. Doctoral Dissertation, Oregon State University. Accessed March 02, 2021.
http://hdl.handle.net/1957/18461.
MLA Handbook (7th Edition):
Nammoonnoy, Jintana. “A photoactivable microfluidic device for heavy metal ion extraction.” 2010. Web. 02 Mar 2021.
Vancouver:
Nammoonnoy J. A photoactivable microfluidic device for heavy metal ion extraction. [Internet] [Doctoral dissertation]. Oregon State University; 2010. [cited 2021 Mar 02].
Available from: http://hdl.handle.net/1957/18461.
Council of Science Editors:
Nammoonnoy J. A photoactivable microfluidic device for heavy metal ion extraction. [Doctoral Dissertation]. Oregon State University; 2010. Available from: http://hdl.handle.net/1957/18461

Hong Kong University of Science and Technology
12.
Tian, Qian CHEM.
Fabrication of microfluidic devices and biomaterial design for biological applications.
Degree: 2017, Hong Kong University of Science and Technology
URL: http://repository.ust.hk/ir/Record/1783.1-102102
;
https://doi.org/10.14711/thesis-b1781150
;
http://repository.ust.hk/ir/bitstream/1783.1-102102/1/th_redirect.html
► Microfluidics is an emerging science and technology that manipulates the fluids in channels of tens of micrometers. It provides better solutions in fields of chemistry,…
(more)
▼ Microfluidics is an emerging science and technology that manipulates the fluids in channels of tens of micrometers. It provides better solutions in fields of chemistry, material science and biology. Its versatile applications involve chemical synthesis, separation and analysis, high-throughput drug screening, bioanalysis, cell biology and tissue engineering, etc. However, it still requires a great deal of work to be done in its manufacturing and downstream applications to make it widely and inexpensively available. This PhD thesis focuses on fabrication of microfluidic devices and biomaterial design for biological applications through conducting three independent projects. Herein, I start from a brief introduction of microfluidics including the fabrication of PDMS-based microfluidics devices and several representative applications. After that, I present the research projects during my PhD study: (1) Microneedle arrays using microlens-based UV lithography, and microneedle arrays are applicable for applications in drug delivery; (2) Microfluidic method for on-demand fluid dispensing using a microcreviced membrane, and this fluid dispensing method is readily to be applied in chemical and biological analysis; (3) Self-crosslinkable chitosan hydrogel for in situ cell encapsulation and subsequent 3D cell culture, which is a promising extracellular matrix (ECM) candidate in the field of tissue engineering. Each project is related to biological applications.
Subjects/Keywords: Microfluidics
; Microfluidic devices
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❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Tian, Q. C. (2017). Fabrication of microfluidic devices and biomaterial design for biological applications. (Thesis). Hong Kong University of Science and Technology. Retrieved from http://repository.ust.hk/ir/Record/1783.1-102102 ; https://doi.org/10.14711/thesis-b1781150 ; http://repository.ust.hk/ir/bitstream/1783.1-102102/1/th_redirect.html
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Tian, Qian CHEM. “Fabrication of microfluidic devices and biomaterial design for biological applications.” 2017. Thesis, Hong Kong University of Science and Technology. Accessed March 02, 2021.
http://repository.ust.hk/ir/Record/1783.1-102102 ; https://doi.org/10.14711/thesis-b1781150 ; http://repository.ust.hk/ir/bitstream/1783.1-102102/1/th_redirect.html.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Tian, Qian CHEM. “Fabrication of microfluidic devices and biomaterial design for biological applications.” 2017. Web. 02 Mar 2021.
Vancouver:
Tian QC. Fabrication of microfluidic devices and biomaterial design for biological applications. [Internet] [Thesis]. Hong Kong University of Science and Technology; 2017. [cited 2021 Mar 02].
Available from: http://repository.ust.hk/ir/Record/1783.1-102102 ; https://doi.org/10.14711/thesis-b1781150 ; http://repository.ust.hk/ir/bitstream/1783.1-102102/1/th_redirect.html.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Tian QC. Fabrication of microfluidic devices and biomaterial design for biological applications. [Thesis]. Hong Kong University of Science and Technology; 2017. Available from: http://repository.ust.hk/ir/Record/1783.1-102102 ; https://doi.org/10.14711/thesis-b1781150 ; http://repository.ust.hk/ir/bitstream/1783.1-102102/1/th_redirect.html
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Hong Kong University of Science and Technology
13.
Tian, Jingxuan PHYS.
A valve-free 2D concentration gradient generator.
Degree: 2016, Hong Kong University of Science and Technology
URL: http://repository.ust.hk/ir/Record/1783.1-97837
;
https://doi.org/10.14711/thesis-b1610623
;
http://repository.ust.hk/ir/bitstream/1783.1-97837/1/th_redirect.html
► This thesis mainly studies topics in microfluidic chips. Microfluidic chip is a promising platform for multiple discipline, like biology, medicine, chemistry and material synthesis. It…
(more)
▼ This thesis mainly studies topics in microfluidic chips. Microfluidic chip is a promising platform for multiple discipline, like biology, medicine, chemistry and material synthesis. It could offer some unique advantages that large scale platforms don’t share. In the following content, a specific aspect of microfluidic chip, the gradient generator is mainly investigated, also some techniques in microfluidics has been discussed. An introduction from general background to specific topics that are related to the research in this thesis has been talked about in Firstly in chapter 1. In chapter 2, the main research project is described in details, including introduction of the present work in this field and the whole process of designing this chip. Then chapter 3 is an extension of one small finding of the 2D concentration gradient generator, which is a hypothesis of the origin of an annoying phenomenon and its potential solution. Chapter 4 is about the techniques that have been investigated throughout the bottom search. They may not be adopted at final stage, however still shows significance as building bricks for the final result. Chapter 5 is summary and perspectives of the work.
Subjects/Keywords: Microfluidic devices
; Microfluidics
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❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Tian, J. P. (2016). A valve-free 2D concentration gradient generator. (Thesis). Hong Kong University of Science and Technology. Retrieved from http://repository.ust.hk/ir/Record/1783.1-97837 ; https://doi.org/10.14711/thesis-b1610623 ; http://repository.ust.hk/ir/bitstream/1783.1-97837/1/th_redirect.html
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Tian, Jingxuan PHYS. “A valve-free 2D concentration gradient generator.” 2016. Thesis, Hong Kong University of Science and Technology. Accessed March 02, 2021.
http://repository.ust.hk/ir/Record/1783.1-97837 ; https://doi.org/10.14711/thesis-b1610623 ; http://repository.ust.hk/ir/bitstream/1783.1-97837/1/th_redirect.html.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Tian, Jingxuan PHYS. “A valve-free 2D concentration gradient generator.” 2016. Web. 02 Mar 2021.
Vancouver:
Tian JP. A valve-free 2D concentration gradient generator. [Internet] [Thesis]. Hong Kong University of Science and Technology; 2016. [cited 2021 Mar 02].
Available from: http://repository.ust.hk/ir/Record/1783.1-97837 ; https://doi.org/10.14711/thesis-b1610623 ; http://repository.ust.hk/ir/bitstream/1783.1-97837/1/th_redirect.html.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Tian JP. A valve-free 2D concentration gradient generator. [Thesis]. Hong Kong University of Science and Technology; 2016. Available from: http://repository.ust.hk/ir/Record/1783.1-97837 ; https://doi.org/10.14711/thesis-b1610623 ; http://repository.ust.hk/ir/bitstream/1783.1-97837/1/th_redirect.html
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Hong Kong University of Science and Technology
14.
Wang, Jing.
A new on-chip immunoassay platform combining yeast surface display and microfluidic system for point-of-care application.
Degree: 2011, Hong Kong University of Science and Technology
URL: http://repository.ust.hk/ir/Record/1783.1-7375
;
https://doi.org/10.14711/thesis-b1155745
;
http://repository.ust.hk/ir/bitstream/1783.1-7375/1/th_redirect.html
► Immunoassay, as a technique that utilizes antibody-antigen interactions for the detection of relevant analytes, plays an important role in our daily life. It can be…
(more)
▼ Immunoassay, as a technique that utilizes antibody-antigen interactions for the detection of relevant analytes, plays an important role in our daily life. It can be used for the quantification of proteins and small molecules in a number of different fields, such as medical diagnosis, food safety and environmental monitoring. The analysis of antibodies directed against specific epitopes with high specificity and sensitivity in human serum is becoming an increasingly critical task in medical and life sciences as the process provides detection of a serologic biomarker which enables early diagnosis in many diseases: for example, infectious diseases such as HIV and hepatitis B, autoimmune diseases such as systemic lupus erythematosus (SLE), as well as allergies and cancers. Recently, there is an urgent global need for point-of-care immunoassays which can be operated by end users since early stage antibody detection plays a crucial role in disease diagnosis and treatment. In this thesis work, we developed a new on-chip immunoassay platform, which combines the yeast surface display (YSD) technique and a microfluidic system. By directly counting genetically-engineered yeast cells bound with target analytes, the immunoassay was realized on a microchip tailored for point-of-care applications. Taking advantage of YSD, antigenic reporters are easily to be obtained by simply yeast culture, and the tedious steps of antigen purification and wet-chemistry processes to immobilize probe antigen can be eliminated. At the same time, with individual cells readily counted by routine microscopy, fewer numbers of cells can be detected than fluorescence or chromophore-based methods. Thus, this method has the inherent characteristic to be highly sensitive. Furthermore, controlled laminar flow can be applied to preferentially remove nonspecifically bound cells, improving the selectivity of the assay. We have built a prototypical microfluidic immunoassay device incorporating functionalized planar surface and fluidic force discrimination, and demonstrated multiplexed antibody detection using bifunctional engineered yeast cells. The magnetic bead-assisted method, which utilizes protein G coupled magnetic beads to capture antibody-labeled cells, improves the sensitivity of the YSD-based microfluidic immunoassay by 100 times and provides a detection limit of 0.5 ng/ml. Alternatively, we have also developed a functionalized micro pillar array (MPA)-based microfluidic immunoassay, utilizing capillary force as the driven power to replace pumping. The high surface area of the MPA results in a high possibility for cells to contact and attach to the pillars, and provides a detection limit of 5 ng/ml. This method is believed to have excellent compatibility with common biopsy used in general hospitals, as well as high potential to be integrated into a portable system. In order to achieve real point-of-care antibody detection that can be operated by end users, we have developed a non-optical YSD based microfluidic immunoassay using micro Coulter particle…
Subjects/Keywords: Immunoassay
; Microfluidic devices
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❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Wang, J. (2011). A new on-chip immunoassay platform combining yeast surface display and microfluidic system for point-of-care application. (Thesis). Hong Kong University of Science and Technology. Retrieved from http://repository.ust.hk/ir/Record/1783.1-7375 ; https://doi.org/10.14711/thesis-b1155745 ; http://repository.ust.hk/ir/bitstream/1783.1-7375/1/th_redirect.html
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Wang, Jing. “A new on-chip immunoassay platform combining yeast surface display and microfluidic system for point-of-care application.” 2011. Thesis, Hong Kong University of Science and Technology. Accessed March 02, 2021.
http://repository.ust.hk/ir/Record/1783.1-7375 ; https://doi.org/10.14711/thesis-b1155745 ; http://repository.ust.hk/ir/bitstream/1783.1-7375/1/th_redirect.html.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Wang, Jing. “A new on-chip immunoassay platform combining yeast surface display and microfluidic system for point-of-care application.” 2011. Web. 02 Mar 2021.
Vancouver:
Wang J. A new on-chip immunoassay platform combining yeast surface display and microfluidic system for point-of-care application. [Internet] [Thesis]. Hong Kong University of Science and Technology; 2011. [cited 2021 Mar 02].
Available from: http://repository.ust.hk/ir/Record/1783.1-7375 ; https://doi.org/10.14711/thesis-b1155745 ; http://repository.ust.hk/ir/bitstream/1783.1-7375/1/th_redirect.html.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Wang J. A new on-chip immunoassay platform combining yeast surface display and microfluidic system for point-of-care application. [Thesis]. Hong Kong University of Science and Technology; 2011. Available from: http://repository.ust.hk/ir/Record/1783.1-7375 ; https://doi.org/10.14711/thesis-b1155745 ; http://repository.ust.hk/ir/bitstream/1783.1-7375/1/th_redirect.html
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Queens University
15.
Swyer, Ian.
Considerations on the use of Impedance Spectroscopy for the Detection of Virions Trapped in Quadrupolar Microelectrode Arrays
.
Degree: Chemical Engineering, 2011, Queens University
URL: http://hdl.handle.net/1974/6528
► The impedance response of a quadrupolar microelectrode array was studied over a wide frequency range to determine whether particles captured at the center of the…
(more)
▼ The impedance response of a quadrupolar microelectrode array was studied over a wide frequency range to determine whether particles captured at the center of the array could be detected impedimetrically. The microelectrode array (denoted as DEP chip) uses dielectrophoretic forces to concentrate particles at its center. Initial results showed that there was a large electrode-silicon-electrode (ESE) capacitance which dominated at high frequencies. This capacitance was reduced by decreasing the electrode area and increasing the insulating layer thickness. These measures however proved fruitless as this capacitance was still significantly greater then the dielectric capacitance of the chip. This ESE capacitance can be eliminated through the use of a glass substrate so that the dielectric response of the chip dominates at higher frequencies. Since the ESE capacitance prevented experimental validation of impedance spectroscopy as a signal transduction method, computer simulations were performed. These simulations indicated that capture with the current DEP chips would not have a significant impact on the impedance of the chip. Decreasing the electrode gap distance and reducing the area of the electrodes, which is recommended for future work, can remedy this.
As measureable changes in the dielectric capacitance of the chip are not possible, a reaction scheme was developed to translate the capture of viral particles into a change in medium conductivity. An ELISA type system was proposed where the viral particles would be functionalized with urease. This uease would then be used to degrade non-ionic urea into ionic products thereby increasing the medium conductivity. A model was formulated to predict the conductivity increase expected for low concentrations, and validated using higher concentrations of biotinylated-urease. Urease from commercial sources proved not to be a viable option as it does not possess a high enough activity to produce a significant conductivity change given the low concentrations of viral particles expected after collection. Urease with suitable activity is produced by the organism Ureaplasma urealyticum which has an activity of 180 000 µmol urea catalyzed min-1 mg urease-1. It is not recommended that this method be pursued further due to technical challenges that would be encountered.
Subjects/Keywords: Impedance
;
Biosensors
;
Microfluidic
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APA (6th Edition):
Swyer, I. (2011). Considerations on the use of Impedance Spectroscopy for the Detection of Virions Trapped in Quadrupolar Microelectrode Arrays
. (Thesis). Queens University. Retrieved from http://hdl.handle.net/1974/6528
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Swyer, Ian. “Considerations on the use of Impedance Spectroscopy for the Detection of Virions Trapped in Quadrupolar Microelectrode Arrays
.” 2011. Thesis, Queens University. Accessed March 02, 2021.
http://hdl.handle.net/1974/6528.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Swyer, Ian. “Considerations on the use of Impedance Spectroscopy for the Detection of Virions Trapped in Quadrupolar Microelectrode Arrays
.” 2011. Web. 02 Mar 2021.
Vancouver:
Swyer I. Considerations on the use of Impedance Spectroscopy for the Detection of Virions Trapped in Quadrupolar Microelectrode Arrays
. [Internet] [Thesis]. Queens University; 2011. [cited 2021 Mar 02].
Available from: http://hdl.handle.net/1974/6528.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Swyer I. Considerations on the use of Impedance Spectroscopy for the Detection of Virions Trapped in Quadrupolar Microelectrode Arrays
. [Thesis]. Queens University; 2011. Available from: http://hdl.handle.net/1974/6528
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Utah
16.
Booth, Ross Hunter.
A microfluidic in vitro model of the blood-brain barrier.
Degree: PhD, Bioengineering, 2014, University of Utah
URL: http://content.lib.utah.edu/cdm/singleitem/collection/etd3/id/3355/rec/53
► The blood-brain barrier (BBB) limits entry of most molecules into the brain and complicates the development of brain-targeting compounds, necessitating novel BBB models. This dissertation…
(more)
▼ The blood-brain barrier (BBB) limits entry of most molecules into the brain and complicates the development of brain-targeting compounds, necessitating novel BBB models. This dissertation describes the first microfluidic BBB model allowing the study of BBB properties in relation to various chemical compounds by enabling tunable wall shear stress (WSS) via dynamic fluid flow, cell-cell interaction through a thin co-culture membrane, time-dependent delivery of test compounds, and integration of sensors into the system, resulting in significant reduction of reagents and cells required and shorter cell seeding time. Use of parallel channels first enabled simultaneous monitoring of multiple cell populations under a wide range (~x15) of WSS.The microfluidic model formed the BBB by incorporating brain endothelial (b.End3) and glial (C6/C8D1A) cells at the intersection of two crossing microchannels, respectively representing luminal and abluminal sides, fabricated in a transparent polydimethylsiloxane (PDMS) substrate utilizing high-precision soft lithography techniques. The utilized cells were adopted from immortalized cells for high consistency over repeated passages and pure and proliferative culture.The developed microfluidic BBB model was validated by (1) expression of tight junction protein ZO-1 and glial protein GFAP by fluorescence imaging, and P-gp activity by Calcein AM, confirming key BBB proteins; (2) high trans-endothelial electrical resistance (TEER) of co-cultures exceeding 250Ωcm2 confirming sufficiently contiguous cell layer formation; (3) chemically-induced barrier modulation, with transient TEER loss by 150μM histamine (~50% for 8-15min), and increase in permeability at elevated pH (10.0); (4) size-dependent (668-70,000Da) compound permeability mimicking in vivo trends; and (5) highly linear correlation (R2>0.85) of clearance rates of seven selected neural drugs with in vivo brain/plasma ratios. We demonstrated the effects of WSS (0-86dyn/cm2) on bEnd.3 properties under increasing WSS, including increase in (6) TEER, (7) cell re-alignment toward flow direction, and (8) protein expression of ZO-1/P-gp, and (9) decrease in tracer permeability.The developed in vitro microfluidic BBB model provides distinct advantages for monitoring and modulating barrier functions and prediction of compound permeability. Thus, it would provide an innovative platform to study mechanisms and pathology of barrier function as well as to assess novel pharmaceuticals early in development for their BBB clearance capabilities.
Subjects/Keywords: Blood-brain barrier; MEMS; Microfluidic
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❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Booth, R. H. (2014). A microfluidic in vitro model of the blood-brain barrier. (Doctoral Dissertation). University of Utah. Retrieved from http://content.lib.utah.edu/cdm/singleitem/collection/etd3/id/3355/rec/53
Chicago Manual of Style (16th Edition):
Booth, Ross Hunter. “A microfluidic in vitro model of the blood-brain barrier.” 2014. Doctoral Dissertation, University of Utah. Accessed March 02, 2021.
http://content.lib.utah.edu/cdm/singleitem/collection/etd3/id/3355/rec/53.
MLA Handbook (7th Edition):
Booth, Ross Hunter. “A microfluidic in vitro model of the blood-brain barrier.” 2014. Web. 02 Mar 2021.
Vancouver:
Booth RH. A microfluidic in vitro model of the blood-brain barrier. [Internet] [Doctoral dissertation]. University of Utah; 2014. [cited 2021 Mar 02].
Available from: http://content.lib.utah.edu/cdm/singleitem/collection/etd3/id/3355/rec/53.
Council of Science Editors:
Booth RH. A microfluidic in vitro model of the blood-brain barrier. [Doctoral Dissertation]. University of Utah; 2014. Available from: http://content.lib.utah.edu/cdm/singleitem/collection/etd3/id/3355/rec/53

University of Alberta
17.
Olson, Steven.
Charge transport in molecular junctions and microfluidic
devices.
Degree: MS, Department of Physics, 2010, University of Alberta
URL: https://era.library.ualberta.ca/files/gx41mj807
► Electro-transmittance of molecular junctions was characterized electrically and studied optically at 410nm and 532nm. Between 1kHz and 100kHz there was no qualitative difference between the…
(more)
▼ Electro-transmittance of molecular junctions was
characterized electrically and studied optically at 410nm and
532nm. Between 1kHz and 100kHz there was no qualitative difference
between the control samples and the molecular junction samples,
however there were difficulties with reproducibility of the
quantitative behaviour, so no hard conclusions could be drawn. A
microfluidic capacitor device was designed and fabricated to study
the electrical double layer, using standard microfabrication
techniques. A complimentary flux corrected transport simulation was
written using the same experimental geometry and the results of
this study found qualitative agreement between the simulation and
experiment. The experiment produced results about the concentration
dependence of the double layer formation time which allows an
estimate of the required frequency of an AC electrical signal for
which the electrical double layer doesn’t have time to form, and
its effects can be ignored.
Subjects/Keywords: microfluidic; molecular junctions; charge transport
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❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Olson, S. (2010). Charge transport in molecular junctions and microfluidic
devices. (Masters Thesis). University of Alberta. Retrieved from https://era.library.ualberta.ca/files/gx41mj807
Chicago Manual of Style (16th Edition):
Olson, Steven. “Charge transport in molecular junctions and microfluidic
devices.” 2010. Masters Thesis, University of Alberta. Accessed March 02, 2021.
https://era.library.ualberta.ca/files/gx41mj807.
MLA Handbook (7th Edition):
Olson, Steven. “Charge transport in molecular junctions and microfluidic
devices.” 2010. Web. 02 Mar 2021.
Vancouver:
Olson S. Charge transport in molecular junctions and microfluidic
devices. [Internet] [Masters thesis]. University of Alberta; 2010. [cited 2021 Mar 02].
Available from: https://era.library.ualberta.ca/files/gx41mj807.
Council of Science Editors:
Olson S. Charge transport in molecular junctions and microfluidic
devices. [Masters Thesis]. University of Alberta; 2010. Available from: https://era.library.ualberta.ca/files/gx41mj807

University of Alberta
18.
Zhang, Le.
Functionalized Bead Based Microchip for Immunoassay and
Virus Immuno-affinity Chromatography.
Degree: MS, Department of Chemistry, 2014, University of Alberta
URL: https://era.library.ualberta.ca/files/cth83kz515
► We demonstrate the functionalization of a highly ordered porous molecular sieving matrix created by colloidal self-assembly (CSA) of 2 micrometer diameter silica particles in microfluidic…
(more)
▼ We demonstrate the functionalization of a highly
ordered porous molecular sieving matrix created by colloidal
self-assembly (CSA) of 2 micrometer diameter silica particles in
microfluidic chips, for highly efficient immuno-capture of viruses.
By tuning the particle size, with appropriate surface chemistry, we
can easily match the pore size which could maximize biological
species-particle wall collision, leading high capture efficiency.
The ordered uniform lattice of pores, which are 15 % of the size of
the particles used, should prove ideal for the capture of viruses
(on the 50-150 nm scale in size). We report on characterization of
capture beds with 300 nm pores. These beds were used to detect
fluorescein using immobilized anti-fluorescein on the bed, and also
to capture and detect type-5 adenovirus in suspension, and in
infected cells using appropriate antibodies. We demonstrate the
performance of the concept using a fully packed column for
fluorescein immunoassay. We used 2 μm carboxylated silica particles
self-assembled into a 6 mm long-bed, modified with EDC/NHS, and
immobilized anti-fluorescein antibody or type-5
adenovirus-recognizing antibody. An electric field was applied to
utilize electro-osmotic flow as the solvent pumping force. A 4.51
nM fluorescein solution was captured by immuno-affinity using
anti-fluorescein antibody, and then released with an eluent to an
empty downstream analysis region to give a very large signal,
providing a positive control. Similar experiments were performed
with type-5 adenovirus, demonstrating detection at a concentration
of 8.3 × 103 viral particles (VP) per milliliter. Adenovirus in
celllysate was also detected in this microchip, with the lowest
concentration detectable at 1.5 × 103 PFU/mL. Combination of
advanced biological detection methods with microfluidics based
extraction and concentration techniques has tremendous potential
for realization of a portable, cost effective and sensitive
pathogen detection system. Taking advantage of the unique fluid
flow characteristics of CSA structures on an even smaller scale
than employed here, faster, more sensitive and more economical
capture and pre-concentration techniques for diagnostic assays for
a wider range of analytes can be developed.
Subjects/Keywords: Microfluidic; Immunoassay; Immuno-affinity Chromatography
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❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Zhang, L. (2014). Functionalized Bead Based Microchip for Immunoassay and
Virus Immuno-affinity Chromatography. (Masters Thesis). University of Alberta. Retrieved from https://era.library.ualberta.ca/files/cth83kz515
Chicago Manual of Style (16th Edition):
Zhang, Le. “Functionalized Bead Based Microchip for Immunoassay and
Virus Immuno-affinity Chromatography.” 2014. Masters Thesis, University of Alberta. Accessed March 02, 2021.
https://era.library.ualberta.ca/files/cth83kz515.
MLA Handbook (7th Edition):
Zhang, Le. “Functionalized Bead Based Microchip for Immunoassay and
Virus Immuno-affinity Chromatography.” 2014. Web. 02 Mar 2021.
Vancouver:
Zhang L. Functionalized Bead Based Microchip for Immunoassay and
Virus Immuno-affinity Chromatography. [Internet] [Masters thesis]. University of Alberta; 2014. [cited 2021 Mar 02].
Available from: https://era.library.ualberta.ca/files/cth83kz515.
Council of Science Editors:
Zhang L. Functionalized Bead Based Microchip for Immunoassay and
Virus Immuno-affinity Chromatography. [Masters Thesis]. University of Alberta; 2014. Available from: https://era.library.ualberta.ca/files/cth83kz515

Oregon State University
19.
Pluess, Christoph.
Application of controlled thermal expansion in diffusion bonding for the high-volume microlamination of MECS devices.
Degree: MS, Industrial Engineering, 2004, Oregon State University
URL: http://hdl.handle.net/1957/30100
Subjects/Keywords: Microfluidic devices
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❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Pluess, C. (2004). Application of controlled thermal expansion in diffusion bonding for the high-volume microlamination of MECS devices. (Masters Thesis). Oregon State University. Retrieved from http://hdl.handle.net/1957/30100
Chicago Manual of Style (16th Edition):
Pluess, Christoph. “Application of controlled thermal expansion in diffusion bonding for the high-volume microlamination of MECS devices.” 2004. Masters Thesis, Oregon State University. Accessed March 02, 2021.
http://hdl.handle.net/1957/30100.
MLA Handbook (7th Edition):
Pluess, Christoph. “Application of controlled thermal expansion in diffusion bonding for the high-volume microlamination of MECS devices.” 2004. Web. 02 Mar 2021.
Vancouver:
Pluess C. Application of controlled thermal expansion in diffusion bonding for the high-volume microlamination of MECS devices. [Internet] [Masters thesis]. Oregon State University; 2004. [cited 2021 Mar 02].
Available from: http://hdl.handle.net/1957/30100.
Council of Science Editors:
Pluess C. Application of controlled thermal expansion in diffusion bonding for the high-volume microlamination of MECS devices. [Masters Thesis]. Oregon State University; 2004. Available from: http://hdl.handle.net/1957/30100

Cornell University
20.
Yu, Yizui.
Passive Microfluidic Mixer Fabrication And Flow Test.
Degree: M.S., Applied Physics, Applied Physics, 2012, Cornell University
URL: http://hdl.handle.net/1813/31192
► This study proposed different materials of microfluidic mixer fabrication and their flow test. Two different design based on a T-mixer were fabricated. J-shaped and vertical…
(more)
▼ This study proposed different materials of
microfluidic mixer fabrication and their flow test. Two different design based on a T-mixer were fabricated. J-shaped and vertical pillar micromixers were fabricated with Zeonor, PDMS, silicon and SU-8. Several methods were used to seal mixer and test the sealing effect. Using Cy5 and fluorescein to measure the mixing time of micromixer. The mixing results shows that the J-shaped micromixer has a good mixing function and a short mixing time.
Advisors/Committee Members: Pollack, Lois (chair), Fuchs, Gregory David (committee member).
Subjects/Keywords: Microfluidic mixer; fabrication; flow test
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❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Yu, Y. (2012). Passive Microfluidic Mixer Fabrication And Flow Test. (Masters Thesis). Cornell University. Retrieved from http://hdl.handle.net/1813/31192
Chicago Manual of Style (16th Edition):
Yu, Yizui. “Passive Microfluidic Mixer Fabrication And Flow Test.” 2012. Masters Thesis, Cornell University. Accessed March 02, 2021.
http://hdl.handle.net/1813/31192.
MLA Handbook (7th Edition):
Yu, Yizui. “Passive Microfluidic Mixer Fabrication And Flow Test.” 2012. Web. 02 Mar 2021.
Vancouver:
Yu Y. Passive Microfluidic Mixer Fabrication And Flow Test. [Internet] [Masters thesis]. Cornell University; 2012. [cited 2021 Mar 02].
Available from: http://hdl.handle.net/1813/31192.
Council of Science Editors:
Yu Y. Passive Microfluidic Mixer Fabrication And Flow Test. [Masters Thesis]. Cornell University; 2012. Available from: http://hdl.handle.net/1813/31192

Vanderbilt University
21.
Ehrman, Jonathan David.
Probing Cell Signaling Networks in Microfluidic Devices.
Degree: PhD, Physics, 2016, Vanderbilt University
URL: http://hdl.handle.net/1803/14608
► Detection of signals is critical for the function of eukaryotic cells. D. discoideum cells are particularly adept at responding chemical gradients, sensing single percent concentration…
(more)
▼ Detection of signals is critical for the function of eukaryotic cells. D. discoideum cells are particularly adept at responding chemical gradients, sensing single percent concentration changes across their body. We measure the ability of the cells to sense gradients through this developmental transition as they change their internal machinery. Additionally, we impair the ability of the cells to modulate the speed of the receptors and measure the effect of a single receptor state on gradient sensing over development.
We find a novel link between mechanotransduction and autophagy through the actin-rich microvillar protrusions lining the gut. These protrusions on the apical cell surface share structural similarities to the mechanosensitive stereocilia in the inner hair cells of the ear. Intestinal epithelial monolayers with microvilli, when exposed to persistent fluid shear stress, developed large vacuoles lined with autophagy associated proteins LC3 and LAMP1. The size and number of vacuoles were suppressed by perturbations to the autophagy pathway, including small molecule inhibitors and LC3 knockdown as well as through perturbations to the microvilli. Our results establish a link between apical shear and autophagic trafficking in intestinal epithelial monolayers.
Advisors/Committee Members: Ken S. Lau (committee member), John P . Wikswo (committee member), Shane M. Hutson (committee member), Erin Rericha (Committee Chair).
Subjects/Keywords: information theory; gut; Microfluidic
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APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Ehrman, J. D. (2016). Probing Cell Signaling Networks in Microfluidic Devices. (Doctoral Dissertation). Vanderbilt University. Retrieved from http://hdl.handle.net/1803/14608
Chicago Manual of Style (16th Edition):
Ehrman, Jonathan David. “Probing Cell Signaling Networks in Microfluidic Devices.” 2016. Doctoral Dissertation, Vanderbilt University. Accessed March 02, 2021.
http://hdl.handle.net/1803/14608.
MLA Handbook (7th Edition):
Ehrman, Jonathan David. “Probing Cell Signaling Networks in Microfluidic Devices.” 2016. Web. 02 Mar 2021.
Vancouver:
Ehrman JD. Probing Cell Signaling Networks in Microfluidic Devices. [Internet] [Doctoral dissertation]. Vanderbilt University; 2016. [cited 2021 Mar 02].
Available from: http://hdl.handle.net/1803/14608.
Council of Science Editors:
Ehrman JD. Probing Cell Signaling Networks in Microfluidic Devices. [Doctoral Dissertation]. Vanderbilt University; 2016. Available from: http://hdl.handle.net/1803/14608

Texas A&M University
22.
Englert, Derek Lynn.
Microfluidic Systems for Investigating Bacterial Chemotaxis and Colonization.
Degree: PhD, Chemical Engineering, 2011, Texas A&M University
URL: http://hdl.handle.net/1969.1/ETD-TAMU-2009-12-7295
► The overall goal of this work was to develop and utilize microfluidic models for investigating bacterial chemotaxis and biofilm formation - phenotypes that play key…
(more)
▼ The overall goal of this work was to develop and utilize
microfluidic models for investigating bacterial chemotaxis and biofilm formation - phenotypes that play key roles in bacterial infections. Classical methods for investigating chemotaxis and biofilm formation have many limitations and drawbacks. These include being unsuitable for investigating the effect of chemorepellents, non-quantitative readouts, and not accounting for interaction between hydrodynamics and biofilm formation. The novel
microfluidic model systems for chemotaxis and biofilm formation developed in this study addresses these drawbacks.
Chemotaxis model system development was done in three stages. We first developed two static chemotaxis model systems - the two fluorophore chemotaxis agarose plug assay and the mu Plug assay - for rapidly determining the extent of chemotaxis in a qualitative manner. A key feature of these model systems was the incorporation of dead cells and differential labeling with green and red fluorescent proteins for partitioning the effects of movement due to fluid flow from chemotaxis. The static systems were used to rapidly screen a wide range of conditions for use in the flow-based mu Flow chemotaxis model system. The effect of four major variables - cell preparation method, gradient strength, flow rate in the device, and imaging position - that influence the chemotactic response in the mu Flow was characterized using the repellent taxis from Ni
2 gradients as the model chemoeffector.
Using the mu Flow chemotaxis device, we investigated the chemotaxis of Escherichia coli RP437 to different signals that are present in the human gastrointestinal tract and are likely to be mediators of infection through their effect on chemotaxis. Our data show that the bacterial signal indole is a repellent, while the signals autoinducer-2 (AI-2) and isatin are attractants for E. coli RP437. However, cells exposed to a competing gradient of indole and either AI-2 or isatin, attracts E. coli. The ?Flow device was also used to refute a long-standing view on how the repellent Ni2 is sensed in E. coli. Our data show that only the Tar chemoreceptor is needed for sensing Ni
2 and the nickel binding protein, NikA, and the Ni
2 transport system proteins, NikB and NikC, are not required for repellent taxis from nickel.
A
microfluidic biofilm model was also developed in this study and used in conjunction with a mathematical model to investigate biofilm formation and quorum sensing in closed systems (where biofilm growth and hydrodynamics are interdependent). The mathematical model predictions were experimentally validated using Pseudomonas aeruginosa PA14 in a
microfluidic biofilm system at various flow rates.
Advisors/Committee Members: Jayaraman, Arul (advisor), Pillai, Suresh D. (committee member), Ugaz, Victor M. (committee member), Wood, Thomas K. (committee member).
Subjects/Keywords: chemotaxis; microfluidic; bacteria; nickel taxis
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❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Englert, D. L. (2011). Microfluidic Systems for Investigating Bacterial Chemotaxis and Colonization. (Doctoral Dissertation). Texas A&M University. Retrieved from http://hdl.handle.net/1969.1/ETD-TAMU-2009-12-7295
Chicago Manual of Style (16th Edition):
Englert, Derek Lynn. “Microfluidic Systems for Investigating Bacterial Chemotaxis and Colonization.” 2011. Doctoral Dissertation, Texas A&M University. Accessed March 02, 2021.
http://hdl.handle.net/1969.1/ETD-TAMU-2009-12-7295.
MLA Handbook (7th Edition):
Englert, Derek Lynn. “Microfluidic Systems for Investigating Bacterial Chemotaxis and Colonization.” 2011. Web. 02 Mar 2021.
Vancouver:
Englert DL. Microfluidic Systems for Investigating Bacterial Chemotaxis and Colonization. [Internet] [Doctoral dissertation]. Texas A&M University; 2011. [cited 2021 Mar 02].
Available from: http://hdl.handle.net/1969.1/ETD-TAMU-2009-12-7295.
Council of Science Editors:
Englert DL. Microfluidic Systems for Investigating Bacterial Chemotaxis and Colonization. [Doctoral Dissertation]. Texas A&M University; 2011. Available from: http://hdl.handle.net/1969.1/ETD-TAMU-2009-12-7295

Penn State University
23.
Saksena, Pulkit.
Study of Condensed Phase Reactions Between Hypergolic Propellants using Microreactors.
Degree: 2014, Penn State University
URL: https://submit-etda.libraries.psu.edu/catalog/22410
► The testing of hypergolic propellants has been carried out for decades and the use of hypergolic propellants at a systems level is very well understood.…
(more)
▼ The testing of hypergolic propellants has been carried out for decades and the use of hypergolic propellants at a systems level is very well understood. Extensive testing has provided the design and construction of rocket engines which have been very reliable. Safety procedures have been developed for the handling and storage for hypergolic propellants, which all too often are highly toxic. But the condensed phase chemistry for these hypergolic propellants is not very well understood. The models in place for ignition and combustion do not handle the coming together of two liquid streams of these hypergols and the two-phase aspects that are expected once the reactions occur. Moreover, it is not clear what makes a fuel and oxidizer pair hypergolic in nature.
The coupling of physical and chemical factors that control the ignition of hypergolic propellants makes the direct study of the transient ignition process difficult. The current study presents a novel method to study hypergolic propellants (very fast liquid reactions) using microreactors instead of conventional drop tests, modified versions thereof or impinging jet tests. Planar counterflow microreactors are used to isolate liquid-phase reactions from secondary gas phase reactions that occur later during the ignition process and thus provide valuable insight in to the pre-ignition mechanism.
The microreactor fabrication, flow field characterization, reactivity results from experiments performed using a variety of fuels and nitric acid as the oxidizer and numerical simulation results of reactive flow in the microreactor are presented in this study. Particle image velocimetry measurements and numerical simulations were conducted to characterize the laminar velocity flow-field in the microreactor and strain rates at the stagnation point along the centerline of the microreactor. Temperature measurements at the stagnation zone, the exit and positions along the length of the microreactor were used as a measure for the extent of the reaction or the heat released from the reaction. For the hypergols, an increase in reactant flow (or equivalently strain rate at the stagnation point) was found to initially increase temperatures at both the stagnation zone as well as along the length of the reactor, but eventually resulted in a decrease in temperature, revealing a maxima in temperature and reactivity. The trends indicated a reaction that was initially diffusion or heat loss controlled, which transitioned towards kinetic control at higher strain (flow) rates. Numerical simulations of the reactive flow in the microreactor stagnation zone were also carried out in which the reaction zone in the microreactor was treated similar to the reaction zone occurring in a counter-flow diffusion flame. These simulations showed that the flow rates at which the peak in temperature occurred was dependent on the rate of the reaction occurring between the hypergol pairs. The numerical simulations also show that heat loss from the top and bottom surfaces can play a role on the temperature…
Advisors/Committee Members: Richard A Yetter, Dissertation Advisor/Co-Advisor, Srinivas A Tadigadapa, Dissertation Advisor/Co-Advisor, Stefan Thynell, Committee Member, Donghai Wang, Committee Member, Siyang Zheng, Committee Member.
Subjects/Keywords: Hypergolic; Propellants; Combustion; Microfluidic; Microreactor
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❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
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Manager
APA (6th Edition):
Saksena, P. (2014). Study of Condensed Phase Reactions Between Hypergolic Propellants using Microreactors. (Thesis). Penn State University. Retrieved from https://submit-etda.libraries.psu.edu/catalog/22410
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Saksena, Pulkit. “Study of Condensed Phase Reactions Between Hypergolic Propellants using Microreactors.” 2014. Thesis, Penn State University. Accessed March 02, 2021.
https://submit-etda.libraries.psu.edu/catalog/22410.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Saksena, Pulkit. “Study of Condensed Phase Reactions Between Hypergolic Propellants using Microreactors.” 2014. Web. 02 Mar 2021.
Vancouver:
Saksena P. Study of Condensed Phase Reactions Between Hypergolic Propellants using Microreactors. [Internet] [Thesis]. Penn State University; 2014. [cited 2021 Mar 02].
Available from: https://submit-etda.libraries.psu.edu/catalog/22410.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Saksena P. Study of Condensed Phase Reactions Between Hypergolic Propellants using Microreactors. [Thesis]. Penn State University; 2014. Available from: https://submit-etda.libraries.psu.edu/catalog/22410
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Penn State University
24.
Poudyal, Ashutosh.
A Study on the Effect of Media Volume Manipulations on Global HUVEC Colony Proliferation Within a Microfluidic Channel.
Degree: 2014, Penn State University
URL: https://submit-etda.libraries.psu.edu/catalog/21829
► Microfluidic-based cell culture is a rapidly developing subject used in studying behavior and external influences on cells on a micro-scale. A majority of modern techniques…
(more)
▼ Microfluidic-based cell culture is a rapidly developing
subject used in studying behavior and external influences on cells on a micro-scale. A majority of modern techniques utilize a perfusion-based culture model where fresh media is continuously passed over growing cells in order to keep them replenished. However, such techniques require intricate setups that have to incorporate syringe pumps, the growth platform, and a culture incubator. To add to the complexity, sterility has to be maintained so as not to contaminate the cells. Unlike perfusion-based techniques, this work demonstrates a novel method to culture cells utilizing a culture media reservoir and nutrient diffusion. Two micropipette tips fixed on the inlet/outlet ends of a Polydimethylsiloxane (PDMS)
microfluidic culture channel are filled with media that passively diffuses throughout the channel to sustain the cells. Optimal times and techniques to replenish the media reservoir were determined through 3D computer simulation and wet bench experimentation. Cell growth on a glass, PDMS, and a Matrigel platform were explored. Ultimately, it was shown that replenishing the culture media within the reservoirs in increments of 5μL allowed for a confluent Human umbilical vein endothelial (HUVE) cell colony to grow in 32 hours of culture. Replenishing the media in 20μL or 50μL increments did not foster global colony growth. Further computational image analysis of cell culture images showed the 5μL culture technique led to roughly two times greater cell colony coverage throughout the growth channel.
Advisors/Committee Members: Siyang Zheng, Thesis Advisor/Co-Advisor, Sheereen Majdzarringhalamaraghy, Thesis Advisor/Co-Advisor, William O Hancock, Thesis Advisor/Co-Advisor.
Subjects/Keywords: HUVEC; microfluidic; cell culture
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APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
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APA (6th Edition):
Poudyal, A. (2014). A Study on the Effect of Media Volume Manipulations on Global HUVEC Colony Proliferation Within a Microfluidic Channel. (Thesis). Penn State University. Retrieved from https://submit-etda.libraries.psu.edu/catalog/21829
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Poudyal, Ashutosh. “A Study on the Effect of Media Volume Manipulations on Global HUVEC Colony Proliferation Within a Microfluidic Channel.” 2014. Thesis, Penn State University. Accessed March 02, 2021.
https://submit-etda.libraries.psu.edu/catalog/21829.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Poudyal, Ashutosh. “A Study on the Effect of Media Volume Manipulations on Global HUVEC Colony Proliferation Within a Microfluidic Channel.” 2014. Web. 02 Mar 2021.
Vancouver:
Poudyal A. A Study on the Effect of Media Volume Manipulations on Global HUVEC Colony Proliferation Within a Microfluidic Channel. [Internet] [Thesis]. Penn State University; 2014. [cited 2021 Mar 02].
Available from: https://submit-etda.libraries.psu.edu/catalog/21829.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Poudyal A. A Study on the Effect of Media Volume Manipulations on Global HUVEC Colony Proliferation Within a Microfluidic Channel. [Thesis]. Penn State University; 2014. Available from: https://submit-etda.libraries.psu.edu/catalog/21829
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Toronto
25.
Tan, Qingyuan.
Development of a Microfluidic Device for Single Cell Specific Membrane Capacitance Quantification.
Degree: 2012, University of Toronto
URL: http://hdl.handle.net/1807/33555
► The specific membrane capacitance (SMC) of biological cell membranes correlates with cells’ electrical activity and morphology, which are physiological markers for cellular phenotype and health.…
(more)
▼ The specific membrane capacitance (SMC) of biological cell membranes correlates with cells’ electrical activity and morphology, which are physiological markers for cellular phenotype and health. Conventionally, SMC measurements are conducted using electro-rotation and Patch-clamping, which entail long time training and stringent operation skills. Both techniques also suffer from limited throughput and lengthy measurement time. In this study, a microfluidic device, which enables impedance spectroscopy measurements, was developed to quantify the SMC of single biological cells. The device has a testing speed of approximately one cell per minute and is relatively easy to operate. Three-dimensional finite element simulations of the microfluidic device confirm the feasibility of this approach. SMC measurement of two AML (Acute Myeloid Leukemia) subtypes and two UCC (Urothelium Cell Carcinoma) subtypes were conducted. Measured SMC results were found to lie in the comparable range with previously reported publications.
MAST
Advisors/Committee Members: Sun, Yu, Mechanical and Industrial Engineering.
Subjects/Keywords: Microfluidic; Cancer cell; 0541
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APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Tan, Q. (2012). Development of a Microfluidic Device for Single Cell Specific Membrane Capacitance Quantification. (Masters Thesis). University of Toronto. Retrieved from http://hdl.handle.net/1807/33555
Chicago Manual of Style (16th Edition):
Tan, Qingyuan. “Development of a Microfluidic Device for Single Cell Specific Membrane Capacitance Quantification.” 2012. Masters Thesis, University of Toronto. Accessed March 02, 2021.
http://hdl.handle.net/1807/33555.
MLA Handbook (7th Edition):
Tan, Qingyuan. “Development of a Microfluidic Device for Single Cell Specific Membrane Capacitance Quantification.” 2012. Web. 02 Mar 2021.
Vancouver:
Tan Q. Development of a Microfluidic Device for Single Cell Specific Membrane Capacitance Quantification. [Internet] [Masters thesis]. University of Toronto; 2012. [cited 2021 Mar 02].
Available from: http://hdl.handle.net/1807/33555.
Council of Science Editors:
Tan Q. Development of a Microfluidic Device for Single Cell Specific Membrane Capacitance Quantification. [Masters Thesis]. University of Toronto; 2012. Available from: http://hdl.handle.net/1807/33555

Victoria University of Wellington
26.
Dhondi, Srikanth.
Numerical Simulations of
Polymers at the Nanoscale.
Degree: 2011, Victoria University of Wellington
URL: http://hdl.handle.net/10063/1753
► In this thesis we study a variety of nanoscale phenomena in certain polymer systems using a combination of numerical simulation methods and mathematical modelling. The…
(more)
▼ In this thesis we study a variety of nanoscale phenomena in certain polymer
systems using a combination of numerical simulation methods and mathematical
modelling. The problems considered are: (a) the mixing behaviour of
polymeric fluids in micro- and nanofluidic devices, (b) capillary absorption of
polymer droplets into narrow capillaries, and (c) modelling the phase separation
and self-assembly behaviour in polymer systems with freely deforming
boundaries. These problems are significant in nanotechnological applications of
polymer-based systems.
First, the mixing behaviour of a polymeric melt over two parallely patternedslip
surfaces is considered. Using molecular dynamics (MD) simulations, it is
shown that mixing is enhanced when the polymer chain size is smaller than the
wavelength of the chemical pattern of the surfaces. An off-set in the upper and
lowerwall patterns improved themixing in the centre of the channel. Application
of a sinusoidally varying body force in addition to the patterned-slip conditions is
shown to enhance mixing further, compared to a constant body force case, with
some limitations. Simulation findings for the constant body force cases are in
qualitative agreement with the continuum theory of Pereira [1]. However, in the
case of a sinusoidally varying body force our simulations do not agree with the
continuum theory. We explain the reasons for the discrepancy between the two
and point out the deficiencies in the continuum theory in predicting the correct
behaviour.
Second, the capillary phenomena of polymer droplets in narrow capillaries
is studied using MD simulations. It is demonstrated that droplets composed of
longer chains require wider tubes for absorption and this result is in agreement
with our continuum modelling. The observed capillary dynamics deviate significantly
from the standard Lucas-Washburn description thus questioning its validity
at the nanoscale. The metastable states during the capillary absorption in
some cases cannot be explained using the existing models of capillary dynamics.
Lastly, the phase separation process in polymer blends between both confined
and unconfined boundaries is studied using Smoothed Particle Hydrodynamics
(SPH). The SPH technique has the advantage of not using a grid to discretize the
spatial domain, which makes it appealing when dealing with problems where
the spatial domain can change with time. The applicability of the SPH method in
describing phase separation in these systems is demonstrated. In particular, its
ability to model freely deforming polymer blends is shown.
Advisors/Committee Members: Hendy, Shaun.
Subjects/Keywords: Molecular dynamics; Microfluidic mixing
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APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Dhondi, S. (2011). Numerical Simulations of
Polymers at the Nanoscale. (Doctoral Dissertation). Victoria University of Wellington. Retrieved from http://hdl.handle.net/10063/1753
Chicago Manual of Style (16th Edition):
Dhondi, Srikanth. “Numerical Simulations of
Polymers at the Nanoscale.” 2011. Doctoral Dissertation, Victoria University of Wellington. Accessed March 02, 2021.
http://hdl.handle.net/10063/1753.
MLA Handbook (7th Edition):
Dhondi, Srikanth. “Numerical Simulations of
Polymers at the Nanoscale.” 2011. Web. 02 Mar 2021.
Vancouver:
Dhondi S. Numerical Simulations of
Polymers at the Nanoscale. [Internet] [Doctoral dissertation]. Victoria University of Wellington; 2011. [cited 2021 Mar 02].
Available from: http://hdl.handle.net/10063/1753.
Council of Science Editors:
Dhondi S. Numerical Simulations of
Polymers at the Nanoscale. [Doctoral Dissertation]. Victoria University of Wellington; 2011. Available from: http://hdl.handle.net/10063/1753

Oregon State University
27.
Siripoorikan, Bunchong.
Flow induced mixing in high aspect ratio microchannels.
Degree: MS, Mechanical Engineering, 2003, Oregon State University
URL: http://hdl.handle.net/1957/31550
► Micro-fluid mixing is an important aspect of many of the various micro-fluidic systems used in biochemical production, biomedical industries, micro-energy systems and some electronic devices.…
(more)
▼ Micro-fluid mixing is an important aspect of many of the various micro-fluidic
systems used in biochemical production, biomedical industries, micro-energy
systems and some electronic devices. Typically, because of size
constraints and laminar flow conditions, different fluids may only have the
opportunity to mix by diffusion, which is extremely rate limited. Therefore, active
or highly effective passive mixing techniques are often required. In this study, two
pulsed injectors are used to actively enhance mixing in a high aspect ratio
microchannel (125 μm deep and 1 mm wide). The main channel has two adjacent
flowing streams with 100% dye and 0% dye concentrations, respectively. Two
injectors (125 μm deep and 250 μm wide) are located on separate sides of the
channel, with one downstream 2 mm (equivalent to two main channel widths or
eight injector widths) from the other. This results in an asymmetric mixing as the
flow proceeds downstream. A dye solution is used to map local mixing
throughout the channel by measuring concentration variations as a function of both
space and time. The primary flow rates are varied from 0.01 to 0.20 ml/min
(Reynolds numbers of 0.3 to 26.6), the injector flow rate ratios are varied from
0.125 to 2, and the pulsing frequencies are varied from 5 to 15 Hz.
Images of the concentration variations within the channel are used to
quantify mixing by calibrating the intensity of the image with the concentration of
the dye solution. The degree of mixing (DoM) is used as a measure of quality and
is defined based on the integration across the channel of the difference between the
local concentration and the 50% concentration values. DoM is normalized by the
50% concentration value and subtracted from one to yield a parameter that varies
from 0 (no mixing) to 1 (perfect mixing). It is shown that there is a high degree of
repeatability of concentration distribution as a function of phase of the pulsing
cycle. A mixing map is constructed over the range of variables tested which
indicates an optimum set of flow and pulsing conditions needed to achieve
maximum mixing in the main channel flow. The flow rate ratio between the
injectors and main channel is found to be the most influential parameter on overall
mixing. The highest DoM in the main channel was found to be 0.89. It is also
noticed that improved mixing can occur at very low flow ratios under a unique set
of primary flow and low frequency pulsing conditions. In general, there is an
inverse relationship between primary flow rate and pulsing frequency to achieve
better overall mixing.
Advisors/Committee Members: Liburdy, James A. (advisor).
Subjects/Keywords: Microfluidic devices
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❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Siripoorikan, B. (2003). Flow induced mixing in high aspect ratio microchannels. (Masters Thesis). Oregon State University. Retrieved from http://hdl.handle.net/1957/31550
Chicago Manual of Style (16th Edition):
Siripoorikan, Bunchong. “Flow induced mixing in high aspect ratio microchannels.” 2003. Masters Thesis, Oregon State University. Accessed March 02, 2021.
http://hdl.handle.net/1957/31550.
MLA Handbook (7th Edition):
Siripoorikan, Bunchong. “Flow induced mixing in high aspect ratio microchannels.” 2003. Web. 02 Mar 2021.
Vancouver:
Siripoorikan B. Flow induced mixing in high aspect ratio microchannels. [Internet] [Masters thesis]. Oregon State University; 2003. [cited 2021 Mar 02].
Available from: http://hdl.handle.net/1957/31550.
Council of Science Editors:
Siripoorikan B. Flow induced mixing in high aspect ratio microchannels. [Masters Thesis]. Oregon State University; 2003. Available from: http://hdl.handle.net/1957/31550

Oregon State University
28.
Enfield, Kent E.
Laminate mixing in microscale fractal-like merging channel networks.
Degree: MS, Mechanical Engineering, 2003, Oregon State University
URL: http://hdl.handle.net/1957/32377
► A two-dimensional model was developed to predict concentration profiles from passive, laminar mixing of concentration layers formed in a fractal-like merging channel network. Both flat…
(more)
▼ A two-dimensional model was developed to predict concentration profiles
from passive, laminar mixing of concentration layers formed in a fractal-like
merging channel network. Both flat and parabolic velocity profiles were used in
the model. A physical experiment was used to confirm the results of the model.
Concentration profiles were acquired in the channels using laser induced
fluorescence. The degree of mixing was defined and used to quantify the mixing in
the test section. Although the results of the experiment follow the trend predicted
by the two-dimensional model, the model under predicts the results of the
experiment. A three-dimensional CFD model of the flow field in the channel
network was used to explain the discrepancies between the two-dimensional model
and the experiment.
For the channel network considered, the degree of mixing is a function of
Peclet number. The effect of geometry on the degree of mixing is investigated
using the two-dimensional model by varying the flow length, the width of the inlet
channels, and the number of branching levels. A non-dimensional parameter is
defined and used to predict an optimum number of branching levels to maximize
mixing for a fixed inlet channel width, total length, and channel depth.
Advisors/Committee Members: Pence, Deborah V. (advisor).
Subjects/Keywords: Microfluidic devices
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❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Enfield, K. E. (2003). Laminate mixing in microscale fractal-like merging channel networks. (Masters Thesis). Oregon State University. Retrieved from http://hdl.handle.net/1957/32377
Chicago Manual of Style (16th Edition):
Enfield, Kent E. “Laminate mixing in microscale fractal-like merging channel networks.” 2003. Masters Thesis, Oregon State University. Accessed March 02, 2021.
http://hdl.handle.net/1957/32377.
MLA Handbook (7th Edition):
Enfield, Kent E. “Laminate mixing in microscale fractal-like merging channel networks.” 2003. Web. 02 Mar 2021.
Vancouver:
Enfield KE. Laminate mixing in microscale fractal-like merging channel networks. [Internet] [Masters thesis]. Oregon State University; 2003. [cited 2021 Mar 02].
Available from: http://hdl.handle.net/1957/32377.
Council of Science Editors:
Enfield KE. Laminate mixing in microscale fractal-like merging channel networks. [Masters Thesis]. Oregon State University; 2003. Available from: http://hdl.handle.net/1957/32377

University of Adelaide
29.
Zhang, Mengjue.
Microfluidic system development for drug delivery.
Degree: 2016, University of Adelaide
URL: http://hdl.handle.net/2440/102614
► Development and application of a microfluidic system for generating drug delivery carriers are investigated in this research. Various types of microfluidic devices are designed and…
(more)
▼ Development and application of a
microfluidic system for generating drug delivery carriers are investigated in this research. Various types of
microfluidic devices are designed and fabricated for peptide nanotubes, liposome vesicles and double emulsions formation. The
microfluidic system offers a better control over the formation process of all three drug delivery carriers. Comparing to traditional methods such as bulk mixing, the process efficiency, size and size distribution of the final products are significantly improved. The results generated show that tuning the flow rate ratios between different reagents from the inlet streams successfully controls the sizes and size distributions of liposomes vesicles. The relationship between the flow rate ratio and the size of the resulting vesicles is established. Macrocycle (AP-169) that was found to self-assemble into an anti-parallel β-sheet nanotube with a triggering agent is successfully synthesized and purified for peptide nanotube self-assembling process. A
microfluidic device is designed and fabricated to control the interaction between AP-169 and its self-assembling triggering agent, dimethyl sulfoxide. Double emulsions with different radii are produced with the
microfluidic system by adjusting the flow rate ratio between each phase of the solution, and changing the wetting properties of the microchannels. The stability of double emulsions is enhanced by introducing various surfactants. The sizes and size distributions of liposomes and double emulsions have been successfully controlled and optimized for drug delivery. In conclusion, various drug delivery carriers have been successfully generated and optimized with a designed and modified
microfluidic system. These products can be further applied in drug encapsulation, biomolecular screening and in vitro compartmentalization in the future.
Advisors/Committee Members: Bi, Jingxiu (advisor), Zhang, Hu (advisor), Biggs, Mark James (advisor), School of Chemical Engineering (school).
Subjects/Keywords: microfluidic; liposomes; double emulsions
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❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Zhang, M. (2016). Microfluidic system development for drug delivery. (Thesis). University of Adelaide. Retrieved from http://hdl.handle.net/2440/102614
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Zhang, Mengjue. “Microfluidic system development for drug delivery.” 2016. Thesis, University of Adelaide. Accessed March 02, 2021.
http://hdl.handle.net/2440/102614.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Zhang, Mengjue. “Microfluidic system development for drug delivery.” 2016. Web. 02 Mar 2021.
Vancouver:
Zhang M. Microfluidic system development for drug delivery. [Internet] [Thesis]. University of Adelaide; 2016. [cited 2021 Mar 02].
Available from: http://hdl.handle.net/2440/102614.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Zhang M. Microfluidic system development for drug delivery. [Thesis]. University of Adelaide; 2016. Available from: http://hdl.handle.net/2440/102614
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Toronto
30.
Crocker, Alana.
Examining the Effect of Laminar Flow on Ex Vivo Pancreatic Islet Associated Endothelial Cells.
Degree: 2010, University of Toronto
URL: http://hdl.handle.net/1807/25460
► Pancreatic islets are heavily vascularized micro-organs containing insulin secreting beta-cells coupled with endothelial cells (EC). These EC slowly deteriorate in static culture, precluding long term…
(more)
▼ Pancreatic islets are heavily vascularized micro-organs containing insulin secreting beta-cells coupled with endothelial cells (EC). These EC slowly deteriorate in static culture, precluding long term study of beta-cell-EC interaction, and likely limiting tissue revascularization post-transplantation. We postulate this EC deterioration is due to an absence of hemodynamics, blood movement. We created a microfluidic device to mimic aspects of hemodynamics, delivering a range of media flow to ex vivo islets. With our resulting desk-top system, we have conducted long term incubations (72 hrs), fixed tissue treatments (maintaining endothelial cell morphology) and real-time live tissue imaging (glucose-stimulated Ca2+-response). Our data show that flow in a microfluidic device maintains EC morphology in ex vivo islets better than non-flowing culture, providing an improved platform to study ex vivo islets and to examine the interaction between beta-cells and EC. Our data also suggest an opportunity to prime islet EC for revascularization using microfluidic flow prior to transplantation.
MAST
Advisors/Committee Members: Rocheleau, Jonathan, Biomedical Engineering.
Subjects/Keywords: Endothelial Cells; Microfluidic; 0541
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❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Crocker, A. (2010). Examining the Effect of Laminar Flow on Ex Vivo Pancreatic Islet Associated Endothelial Cells. (Masters Thesis). University of Toronto. Retrieved from http://hdl.handle.net/1807/25460
Chicago Manual of Style (16th Edition):
Crocker, Alana. “Examining the Effect of Laminar Flow on Ex Vivo Pancreatic Islet Associated Endothelial Cells.” 2010. Masters Thesis, University of Toronto. Accessed March 02, 2021.
http://hdl.handle.net/1807/25460.
MLA Handbook (7th Edition):
Crocker, Alana. “Examining the Effect of Laminar Flow on Ex Vivo Pancreatic Islet Associated Endothelial Cells.” 2010. Web. 02 Mar 2021.
Vancouver:
Crocker A. Examining the Effect of Laminar Flow on Ex Vivo Pancreatic Islet Associated Endothelial Cells. [Internet] [Masters thesis]. University of Toronto; 2010. [cited 2021 Mar 02].
Available from: http://hdl.handle.net/1807/25460.
Council of Science Editors:
Crocker A. Examining the Effect of Laminar Flow on Ex Vivo Pancreatic Islet Associated Endothelial Cells. [Masters Thesis]. University of Toronto; 2010. Available from: http://hdl.handle.net/1807/25460
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