subject:(Medicine AND Health Sciences)

1. Pangrazzi, Elizabeth. The Rural Provider's Perspective| Conversations With Patients About Mental Health.

Degree: 2017, Union Institute and University

URL: http://pqdtopen.proquest.com/#viewpdf?dispub=10624346

► The rural primary care provider is likely to treat patients with mental health issues in rural populations due to lack of mental health providers,… (more)

Subjects/Keywords: Mental health; Medicine; Health sciences

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APA (6^th Edition):

Pangrazzi, E. (2017). The Rural Provider's Perspective| Conversations With Patients About Mental Health. (Thesis). Union Institute and University. Retrieved from http://pqdtopen.proquest.com/#viewpdf?dispub=10624346

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Pangrazzi, Elizabeth. “The Rural Provider's Perspective| Conversations With Patients About Mental Health.” 2017. Thesis, Union Institute and University. Accessed March 02, 2021. http://pqdtopen.proquest.com/#viewpdf?dispub=10624346.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Pangrazzi, Elizabeth. “The Rural Provider's Perspective| Conversations With Patients About Mental Health.” 2017. Web. 02 Mar 2021.

Vancouver:

Pangrazzi E. The Rural Provider's Perspective| Conversations With Patients About Mental Health. [Internet] [Thesis]. Union Institute and University; 2017. [cited 2021 Mar 02]. Available from: http://pqdtopen.proquest.com/#viewpdf?dispub=10624346.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Pangrazzi E. The Rural Provider's Perspective| Conversations With Patients About Mental Health. [Thesis]. Union Institute and University; 2017. Available from: http://pqdtopen.proquest.com/#viewpdf?dispub=10624346

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

2. Gottlieb, Eric Raphael. Humoral Immune Responses to a Malaria Vaccine Candidate| Towards a Correlate of Vaccine-Induced Protection.

Degree: 2012, University of Maryland, Baltimore

URL: http://pqdtopen.proquest.com/#viewpdf?dispub=1512511

► Introduction: Identifying immune correlates of protection is a priority for malaria vaccine research. A successful pediatric Phase 2 clinical trial in Mali of FMP2.1/AS02_A,… (more)

▼ Introduction: Identifying immune correlates of protection is a priority for malaria vaccine research. A successful pediatric Phase 2 clinical trial in Mali of FMP2.1/AS02_A, a recombinant apical membrane antigen 1 (AMA1)-based vaccine candidate, provided a source of serum samples from subjects who may have developed vaccine-induced, strain-specific protective immunity to clinical malaria illness. We studied IgG subclass and avidity patterns of antibodies to the malaria protein AMA1 in a subset of participants, with the objective of identifying immune responses that may be associated with protection against malaria. We hypothesized that the AMA1 vaccine candidate would induce production of cytophilic antibody subclasses IgG1 and IgG3, as well as overall IgG avidity maturation. Methods: Titers of IgG1, IgG2, IgG3, and IgG4, as well as avidity of antibodies to AMA1, were determined by ELISA for ten AMA1 vaccine recipients and ten control subjects who had been randomized to receive a rabies vaccine in this double-blind trial at days 0, 90, and 150 after the first of three vaccinations. To identify statistically significant differences between the groups, responses in vaccine recipients were evaluated longitudinally and compared with responses in control subjects. Results: IgG1, IgG2, IgG3, and IgG4 were induced more strongly in vaccine recipients than in control subjects. Additionally, vaccine recipients had higher ratios of cytophilic to non-cytophilic antibodies than control subjects. Avidity indices were not significantly different between the two groups at the three time points tested, and there were no significant differences in avidity between time points in either group. Conclusion: Contrary to our hypothesis, both cytophilic and non-cytophilic antibodies were induced by the FMP2.1/AS02A vaccine candidate and immunization did not appear to stimulate avidity maturation. Therefore, IgG1, IgG2, IgG3, and IgG4 titers are candidate variables for a humoral immune correlate of vaccine-induced protection. These results are among the first reports of the subclasses and avidity of antibodies produced in response to a malaria vaccine candidate with allele-specific protective efficacy.

Subjects/Keywords: Health Sciences; Medicine and Surgery

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APA (6^th Edition):

Gottlieb, E. R. (2012). Humoral Immune Responses to a Malaria Vaccine Candidate| Towards a Correlate of Vaccine-Induced Protection. (Thesis). University of Maryland, Baltimore. Retrieved from http://pqdtopen.proquest.com/#viewpdf?dispub=1512511

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Gottlieb, Eric Raphael. “Humoral Immune Responses to a Malaria Vaccine Candidate| Towards a Correlate of Vaccine-Induced Protection.” 2012. Thesis, University of Maryland, Baltimore. Accessed March 02, 2021. http://pqdtopen.proquest.com/#viewpdf?dispub=1512511.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Gottlieb, Eric Raphael. “Humoral Immune Responses to a Malaria Vaccine Candidate| Towards a Correlate of Vaccine-Induced Protection.” 2012. Web. 02 Mar 2021.

Vancouver:

Gottlieb ER. Humoral Immune Responses to a Malaria Vaccine Candidate| Towards a Correlate of Vaccine-Induced Protection. [Internet] [Thesis]. University of Maryland, Baltimore; 2012. [cited 2021 Mar 02]. Available from: http://pqdtopen.proquest.com/#viewpdf?dispub=1512511.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Gottlieb ER. Humoral Immune Responses to a Malaria Vaccine Candidate| Towards a Correlate of Vaccine-Induced Protection. [Thesis]. University of Maryland, Baltimore; 2012. Available from: http://pqdtopen.proquest.com/#viewpdf?dispub=1512511

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

3. DeRosa, Megan Sullivan. Golden Threads| Shared Themes in Holistic Health and Healing Modalities.

Degree: 2013, Prescott College

URL: http://pqdtopen.proquest.com/#viewpdf?dispub=1530859

► There is growing popularity and widespread use of holistic health and healing modalities, which emphasize a mind-body-spirit approach to supporting or restoring well-being and… (more)

Subjects/Keywords: Health Sciences, Alternative Medicine; Spirituality

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APA (6^th Edition):

DeRosa, M. S. (2013). Golden Threads| Shared Themes in Holistic Health and Healing Modalities. (Thesis). Prescott College. Retrieved from http://pqdtopen.proquest.com/#viewpdf?dispub=1530859

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

DeRosa, Megan Sullivan. “Golden Threads| Shared Themes in Holistic Health and Healing Modalities.” 2013. Thesis, Prescott College. Accessed March 02, 2021. http://pqdtopen.proquest.com/#viewpdf?dispub=1530859.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

DeRosa, Megan Sullivan. “Golden Threads| Shared Themes in Holistic Health and Healing Modalities.” 2013. Web. 02 Mar 2021.

Vancouver:

DeRosa MS. Golden Threads| Shared Themes in Holistic Health and Healing Modalities. [Internet] [Thesis]. Prescott College; 2013. [cited 2021 Mar 02]. Available from: http://pqdtopen.proquest.com/#viewpdf?dispub=1530859.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

DeRosa MS. Golden Threads| Shared Themes in Holistic Health and Healing Modalities. [Thesis]. Prescott College; 2013. Available from: http://pqdtopen.proquest.com/#viewpdf?dispub=1530859

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

4. Oliver, Brant J. Modifiable Factor Relationships with Biopsychosocial Disability in Multiple Sclerosis.

Degree: 2017, Dartmouth College

URL: http://pqdtopen.proquest.com/#viewpdf?dispub=10184281

► Problem Statement: Multiple sclerosis (MS) is a chronic disease of the central nervous system and is among the most common neurological disorders in adults.… (more)

▼ Problem Statement: Multiple sclerosis (MS) is a chronic disease of the central nervous system and is among the most common neurological disorders in adults. MS causes significant disability and has no cure. Neurological disease severity is thought to contribute to disability but may not be the main determinant. Individual and environmental factors may modify the relationship between disease severity and disability or directly influence it. Research simultaneously relating these factors in MS has not yet been conducted. Methods: Participants completed an internet questionnaire and a telephone-administered cognitive recall performance task. Clinical data were extracted from a data registry maintained by the Multiple Sclerosis Center at Dartmouth-Hitchcock Medical Center (DHMC) in Lebanon, New Hampshire. Correlation and multiple regression analyses were conducted to determine the relationships between disease severity, individual factors, environmental factors and biopsychosocial disability (BPSD). Results: One hundred-eighty (180) adults with relapsing forms of MS participated in the study, representing approximately 15% of the total MS patient population followed by the database registry at DHMC. The study population was 76% female, had 14.6 years of education, a mean annual income of $35,000, was 75% relapsing-remitting MS (RRMS), had a mean EDSS of 2.4, and a mean ARR of 0.21. In multiple regression analyses, disease severity explained 3%, demographic variables 6%, individual factors 69%, and environmental factors 64% of the variance in BPSD, respectively. The final model incorporating disease severity, individual factors, and environmental factors explained 74% of the variance in BPSD. Depression and environmental status demonstrated the strongest associations with BPSD. Conclusions: Neuropsychiatric status, bowel and bladder function, self-efficacy, social support, and environmental status are key factors associated with BPSD. These factors are modifiable by medical, psychosocial, and/or case management interventions. This study has three major limitations: (1) inability to predict causality or directionality of associations; (2) susceptibility to self-report bias; and (3) susceptibility to temporal bias. This study provides a basis for continued research on biopsychosocial disability reduction and to related healthcare quality improvement, shared decision making, and health policy initiatives.

Subjects/Keywords: Medicine; Health sciences; Nursing

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APA (6^th Edition):

Oliver, B. J. (2017). Modifiable Factor Relationships with Biopsychosocial Disability in Multiple Sclerosis. (Thesis). Dartmouth College. Retrieved from http://pqdtopen.proquest.com/#viewpdf?dispub=10184281

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Oliver, Brant J. “Modifiable Factor Relationships with Biopsychosocial Disability in Multiple Sclerosis.” 2017. Thesis, Dartmouth College. Accessed March 02, 2021. http://pqdtopen.proquest.com/#viewpdf?dispub=10184281.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Oliver, Brant J. “Modifiable Factor Relationships with Biopsychosocial Disability in Multiple Sclerosis.” 2017. Web. 02 Mar 2021.

Vancouver:

Oliver BJ. Modifiable Factor Relationships with Biopsychosocial Disability in Multiple Sclerosis. [Internet] [Thesis]. Dartmouth College; 2017. [cited 2021 Mar 02]. Available from: http://pqdtopen.proquest.com/#viewpdf?dispub=10184281.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Oliver BJ. Modifiable Factor Relationships with Biopsychosocial Disability in Multiple Sclerosis. [Thesis]. Dartmouth College; 2017. Available from: http://pqdtopen.proquest.com/#viewpdf?dispub=10184281

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of California – Irvine

5. Jimenez, Krystal Alicia. Risk Factors Associated with Chronic Kidney Disease of Unknown Etiology (CKDu): A Study of Patients from an Occupational Health Clinic in Rural Nicaragua.

Degree: Biomedical and Translational Science, 2016, University of California – Irvine

URL: http://www.escholarship.org/uc/item/2fc4b3zx

► Contrary to the conventional definition of CKD that is observed in developed countries, studies on CKD in developing countries demonstrate an atypical presentation of the… (more)

Subjects/Keywords: Epidemiology; Medicine; Health sciences

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APA (6^th Edition):

Jimenez, K. A. (2016). Risk Factors Associated with Chronic Kidney Disease of Unknown Etiology (CKDu): A Study of Patients from an Occupational Health Clinic in Rural Nicaragua. (Thesis). University of California – Irvine. Retrieved from http://www.escholarship.org/uc/item/2fc4b3zx

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Jimenez, Krystal Alicia. “Risk Factors Associated with Chronic Kidney Disease of Unknown Etiology (CKDu): A Study of Patients from an Occupational Health Clinic in Rural Nicaragua.” 2016. Thesis, University of California – Irvine. Accessed March 02, 2021. http://www.escholarship.org/uc/item/2fc4b3zx.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Jimenez, Krystal Alicia. “Risk Factors Associated with Chronic Kidney Disease of Unknown Etiology (CKDu): A Study of Patients from an Occupational Health Clinic in Rural Nicaragua.” 2016. Web. 02 Mar 2021.

Vancouver:

Jimenez KA. Risk Factors Associated with Chronic Kidney Disease of Unknown Etiology (CKDu): A Study of Patients from an Occupational Health Clinic in Rural Nicaragua. [Internet] [Thesis]. University of California – Irvine; 2016. [cited 2021 Mar 02]. Available from: http://www.escholarship.org/uc/item/2fc4b3zx.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Jimenez KA. Risk Factors Associated with Chronic Kidney Disease of Unknown Etiology (CKDu): A Study of Patients from an Occupational Health Clinic in Rural Nicaragua. [Thesis]. University of California – Irvine; 2016. Available from: http://www.escholarship.org/uc/item/2fc4b3zx

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

6. Hebbrecht, Tim. Nanobody derivatization for molecular study and perturbation of cancer cell invadopodia.

Degree: 2020, Ghent University

URL: http://hdl.handle.net/1854/LU-8669654

► Metastasis is a known lethal factor for cancer patients which is still not evident to prevent. It is one of the hallmarks of cancer. Metastasis… (more)

▼ Metastasis is a known lethal factor for cancer patients which is still not evident to prevent. It is one of the hallmarks of cancer. Metastasis is the process in which cancer cells escape from the primary tumor to disseminate through the body and to create a secondary tumor. To succeed in the spreading, such cancer cells hijack the normal cellular functions to become motile and invasive. Those invasive properties are a result of the actin cytoskeleton changes. Invasive cancer cells use specialised actin based membrane structures, called invadopodia, to facilitate their escape from a primary tumor leading to the dissemination. To study these invadopodia, the nanobody technology is utilised. Nanobodies are the smallest antigen binding fragment derived from the variable part of Camelidae heavy chain antibodies. Because of the high specificity and selectivity of nanobodies, it is possible to target specific invadopodium proteins which aid the study of the structure and the regulation of invadopodia as well as the contribution to invasion in the metastatic pathway. The aim of this study is to reveal the function of neural Wiskott-Aldrich syndrome protein, N-WASp, in the invadopodium pathway by using nanobodies. N-WASp is one of the key players in the metastatic pathway through invadopodia due to its role in the actin polymerisation. The second goal of the study is to create a tool that enables a more accurate imaging compared to the existing methods by using the nanobody technology. In the first part, nanobodies were generated to target the C-terminal domain of N-WASp, the VCA domain. Through its VCA domain, N¬-WASp is able to bind directly to actin monomers and Arp2/3 (actin related protein 2/3). Together they form a complex which is the precursor for a fast actin polymerisation. This is required to start the formation of an invadopodium. Following immunisation, a series of possible nanobodies were generated and had to be characterised first. After the characterisation and the measurement of the binding capacities, the nanobodies were implemented to analyse the role of N-WASp in the invadopodium pathway. The VCA nanobodies were able to create inhibiting effects by reducing the number of invadopodia. However, they exert only small influence on the extracellular matrix degradation. Those results lead to the hypothesis that N-WASp found its main purpose in the invadopodium formation. Due to less invadopodia, the ECM degradation is slightly decreased as well. In the second part, the nanobody technology is expanded to create a better imaging tool. Since the nanobodies are smaller compared to conventional antibodies, they enable a reduction in distance between the target and a dye (also known as linkage error). This results in higher resolution properties compared to the existing methods (e.g. conventional immunostaining, fusion proteins …). In this part, two ways are presented to attach a dye to a recombinant nanobody: by using the sortase A enzyme or by incorporating para-azido phenylalanine, an unnatural amino acid. The… Advisors/Committee Members: Gettemans, Jan.

Subjects/Keywords: Medicine and Health Sciences

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APA (6^th Edition):

Hebbrecht, T. (2020). Nanobody derivatization for molecular study and perturbation of cancer cell invadopodia. (Thesis). Ghent University. Retrieved from http://hdl.handle.net/1854/LU-8669654

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Hebbrecht, Tim. “Nanobody derivatization for molecular study and perturbation of cancer cell invadopodia.” 2020. Thesis, Ghent University. Accessed March 02, 2021. http://hdl.handle.net/1854/LU-8669654.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Hebbrecht, Tim. “Nanobody derivatization for molecular study and perturbation of cancer cell invadopodia.” 2020. Web. 02 Mar 2021.

Vancouver:

Hebbrecht T. Nanobody derivatization for molecular study and perturbation of cancer cell invadopodia. [Internet] [Thesis]. Ghent University; 2020. [cited 2021 Mar 02]. Available from: http://hdl.handle.net/1854/LU-8669654.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Hebbrecht T. Nanobody derivatization for molecular study and perturbation of cancer cell invadopodia. [Thesis]. Ghent University; 2020. Available from: http://hdl.handle.net/1854/LU-8669654

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Loyola University Chicago

7. Nawara, Stephen. Characterization of the Effect of Anti-Nogo-A Antibody Treatment on Sensorimotor Recovery After Traumatic Brain Injury.

Degree: PhD, Pharmacology and Experimental Therapeutics, 2015, Loyola University Chicago

URL: https://ecommons.luc.edu/luc_diss/1650

► Estimates indicate that over 5 million Americans are currently living with Traumatic Brain Injury (TBI) related disability. Much effort has been put into enhancing… (more)

▼ Estimates indicate that over 5 million Americans are currently living with Traumatic Brain Injury (TBI) related disability. Much effort has been put into enhancing functional recovery with physical therapy and other rehabilitative approaches. These strategies are thought to function by facilitating the brain’s innate capacity for neuroregeneration and neuroplasticity. However, the success of these strategies may be limited due the presence of growth- and plasticity- inhibiting factors expressed in the adult brain. The presence of the membrane protein Nogo-A has been associated with reduced neurite outgrowth in vitro, identifying it as one such molecule. In addition, rodents treated with antibodies raised against Nogo-A have been reported to show a greater degree of axonal growth and motor performance after a variety of injuries to the spinal cord and brain. Here, the effect of anti-Nogo-A immunotherapy was studied using the controlled cortical impact (CCI) model of TBI. Rats received CCIs to the sensorimotor cortex opposite the preferred forelimb. Beginning one week later, anti-Nogo-A antibody (11c7) was administered to the lateral cerebral ventricle for two weeks. Recovery of these animals was assessed using the skilled reaching task and compared to controls from one day post-injury until eight weeks later. The anti-Nogo-A treated animals recovered an average of 90% baseline skill, while controls recovered only 60%, suggesting that the treatment may be beneficial. However, this conclusion depends on the assumption that performance on the reaching task is a measure of capability. If so, after a period of re-learning the skill, the individual rat should enter a plateau phase that reflects maximal performance. During this phase, the distribution of day-to-day success rate for each individual should follow a specific probability distribution (binomial). However, in our study the scores during the plateau phase were often much more consistent than predicted, a strong indication that capability was not measured. Further, neither the final performance nor profile of recovery were uniform within each group. When fit with a sigmoidal curve, the distribution of the plateau skill levels appeared multimodal. It is possible that performance was determined by some neurological characteristic of the rats, perhaps related to the brain injury. However, total lesion size and cortical lesion location were not well correlated with performance and could not explain the greater degree of recovery observed for anti-Nogo-A treated animals. Another possibility is that performance was determined by neuroplastic processes, previously proposed to be influenced by anti-Nogo-A antibody. To this end, the brains of the same rats were stained using the Golgi-Cox procedure. The degree of dendritic branching and soma area was measured for pyramidal neurons from the contra-lesional cortex. However, these measurements also did not correlate with performance on the reaching task nor differ between groups. While any…

Subjects/Keywords: Medicine and Health Sciences

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APA (6^th Edition):

Nawara, S. (2015). Characterization of the Effect of Anti-Nogo-A Antibody Treatment on Sensorimotor Recovery After Traumatic Brain Injury. (Doctoral Dissertation). Loyola University Chicago. Retrieved from https://ecommons.luc.edu/luc_diss/1650

Chicago Manual of Style (16^th Edition):

Nawara, Stephen. “Characterization of the Effect of Anti-Nogo-A Antibody Treatment on Sensorimotor Recovery After Traumatic Brain Injury.” 2015. Doctoral Dissertation, Loyola University Chicago. Accessed March 02, 2021. https://ecommons.luc.edu/luc_diss/1650.

MLA Handbook (7^th Edition):

Nawara, Stephen. “Characterization of the Effect of Anti-Nogo-A Antibody Treatment on Sensorimotor Recovery After Traumatic Brain Injury.” 2015. Web. 02 Mar 2021.

Vancouver:

Nawara S. Characterization of the Effect of Anti-Nogo-A Antibody Treatment on Sensorimotor Recovery After Traumatic Brain Injury. [Internet] [Doctoral dissertation]. Loyola University Chicago; 2015. [cited 2021 Mar 02]. Available from: https://ecommons.luc.edu/luc_diss/1650.

Council of Science Editors:

Nawara S. Characterization of the Effect of Anti-Nogo-A Antibody Treatment on Sensorimotor Recovery After Traumatic Brain Injury. [Doctoral Dissertation]. Loyola University Chicago; 2015. Available from: https://ecommons.luc.edu/luc_diss/1650

University of Louisville

8. Hollis, Elizabeth Marie. Diffusion-weighted magnetic resonance imaging in diagnosing graft dysfunction : a non-invasive alternative to renal biopsy.

Degree: MS, 2017, University of Louisville

URL: 10.18297/etd/2661 ; https://ir.library.louisville.edu/etd/2661

► The thesis is divided into three parts. The first part focuses on background information including how the kidney functions, diseases, and available kidney disease… (more)

Subjects/Keywords: Medicine and Health Sciences

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APA (6^th Edition):

Hollis, E. M. (2017). Diffusion-weighted magnetic resonance imaging in diagnosing graft dysfunction : a non-invasive alternative to renal biopsy. (Masters Thesis). University of Louisville. Retrieved from 10.18297/etd/2661 ; https://ir.library.louisville.edu/etd/2661

Chicago Manual of Style (16^th Edition):

Hollis, Elizabeth Marie. “Diffusion-weighted magnetic resonance imaging in diagnosing graft dysfunction : a non-invasive alternative to renal biopsy.” 2017. Masters Thesis, University of Louisville. Accessed March 02, 2021. 10.18297/etd/2661 ; https://ir.library.louisville.edu/etd/2661.

MLA Handbook (7^th Edition):

Hollis, Elizabeth Marie. “Diffusion-weighted magnetic resonance imaging in diagnosing graft dysfunction : a non-invasive alternative to renal biopsy.” 2017. Web. 02 Mar 2021.

Vancouver:

Hollis EM. Diffusion-weighted magnetic resonance imaging in diagnosing graft dysfunction : a non-invasive alternative to renal biopsy. [Internet] [Masters thesis]. University of Louisville; 2017. [cited 2021 Mar 02]. Available from: 10.18297/etd/2661 ; https://ir.library.louisville.edu/etd/2661.

Council of Science Editors:

Hollis EM. Diffusion-weighted magnetic resonance imaging in diagnosing graft dysfunction : a non-invasive alternative to renal biopsy. [Masters Thesis]. University of Louisville; 2017. Available from: 10.18297/etd/2661 ; https://ir.library.louisville.edu/etd/2661

Texas Medical Center

9. Fletcher, Eliot S. ORGAN SPECIFIC VASCULAR RESPONSE TO FIBROSIS AFFECTS BREAST CANCER METASTATIC ORGANOTROPISM.

Degree: PhD, 2016, Texas Medical Center

URL: https://digitalcommons.library.tmc.edu/utgsbs_dissertations/723

► The solid tumor microenvironment, pre-metastatic niche, and fibrotic environment are known to have significant biochemical and biomechanical similarities to the fibrotic environment. All have… (more)

▼ The solid tumor microenvironment, pre-metastatic niche, and fibrotic environment are known to have significant biochemical and biomechanical similarities to the fibrotic environment. All have significantly increased levels of factors such as TGFβ, HIF1α, TNFα, PDGF, VEGF, FGF, interleukins and other growth factors that are known to be pro-tumorigenic. Clinical and basic science research has shown that fibrosis presents an environment that favors tumor growth, such as hepatocellular carcinoma being commonly preceded by liver cirrhosis, or bleomycin induced lung fibrosis enhancing pulmonary metastasis in mouse models of breast cancer. In addition to the evidence indicating that fibrosis enhances primary tumor growth and metastasis it is also well characterized that primary tumor metastasis has specific organotropism, for example breast cancer commonly spreads to the lungs, brain, bone, liver and lymph nodes. However, whether non-organtropic fibrosis can redirect metastasis to the damaged organ has not been investigated. To elucidate the fibrotic effect on tumor organotropism we induced fibrosis in the organotropic lungs and in the non-organotropic kidney of two mouse models of breast cancer, the 4T1 murine cancer cell line model and the genetic MMTV-Pymt model, both of which are known to metastasize. Using histopathology, microarrays, gene expression by polymerase chain reaction, ELISA, chemokine array, and in vitro experiments we demonstrate that despite the pro-tumorigenic environment, kidney fibrosis does not redirect metastasis to the non-organotropic damaged organ. However, mice with kidney fibrosis had increased metastasis to their lungs. Furthermore, we found that kidney fibrosis increases the circulating levels of the pro-angiogenic factor Angiopoietin 2 that increased vascular permeability of the lungs, but not the kidneys. In fact, while fibrotic lungs showed decreased expression of endothelial tight gap junction protein Claudin-5, the fibrotic kidneys had an elevated expression of Claudin-5. Our findings suggest that despite the similarities between fibrosis, the tumor microenvironment and the pre-metastatic niche, that while it can enhance tropic metastatic disease, it cannot redirect organotropism indicating that other factors must be involved in directing organotropism. Here we report that tumor organotropism may be a result of organ specific vascular responses to excess circulating factors and increased fibrotic factors. These findings indicate that organotropism is directly related to and as a result of organ specific vascular alterations. Advisors/Committee Members: Raghu Kalluri, Valerie LeBleu, Menasche Bar Eli.

Subjects/Keywords: Medicine and Health Sciences

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APA (6^th Edition):

Fletcher, E. S. (2016). ORGAN SPECIFIC VASCULAR RESPONSE TO FIBROSIS AFFECTS BREAST CANCER METASTATIC ORGANOTROPISM. (Doctoral Dissertation). Texas Medical Center. Retrieved from https://digitalcommons.library.tmc.edu/utgsbs_dissertations/723

Chicago Manual of Style (16^th Edition):

Fletcher, Eliot S. “ORGAN SPECIFIC VASCULAR RESPONSE TO FIBROSIS AFFECTS BREAST CANCER METASTATIC ORGANOTROPISM.” 2016. Doctoral Dissertation, Texas Medical Center. Accessed March 02, 2021. https://digitalcommons.library.tmc.edu/utgsbs_dissertations/723.

MLA Handbook (7^th Edition):

Fletcher, Eliot S. “ORGAN SPECIFIC VASCULAR RESPONSE TO FIBROSIS AFFECTS BREAST CANCER METASTATIC ORGANOTROPISM.” 2016. Web. 02 Mar 2021.

Vancouver:

Fletcher ES. ORGAN SPECIFIC VASCULAR RESPONSE TO FIBROSIS AFFECTS BREAST CANCER METASTATIC ORGANOTROPISM. [Internet] [Doctoral dissertation]. Texas Medical Center; 2016. [cited 2021 Mar 02]. Available from: https://digitalcommons.library.tmc.edu/utgsbs_dissertations/723.

Council of Science Editors:

Fletcher ES. ORGAN SPECIFIC VASCULAR RESPONSE TO FIBROSIS AFFECTS BREAST CANCER METASTATIC ORGANOTROPISM. [Doctoral Dissertation]. Texas Medical Center; 2016. Available from: https://digitalcommons.library.tmc.edu/utgsbs_dissertations/723

University of Wisconsin – Milwaukee

10. Chakraborty, Advaita. The Potential Role of AIRAP/AIRAPL in Ameliorating the Toxicity Related to Amyloid-beta (aβ) in Mammalian Neuronal Cells.

Degree: MS, Biomedical Sciences, 2017, University of Wisconsin – Milwaukee

URL: https://dc.uwm.edu/etd/1593

► Alzheimer’s disease is an irreversible and progressive brain disorder that affects memory and thinking skills. It is considered to be one of the most… (more)

▼ Alzheimer’s disease is an irreversible and progressive brain disorder that affects memory and thinking skills. It is considered to be one of the most important causes of dementia in older adults. The disease is accompanied by accumulation of amyloid beta (Aβ) plaques in the brains of patients and presence of neurofibrillary tangles (NFTs) in the nerve tissue. The tangles are formed by a protein known as protein-tau (p-tau). Aβ plaques are responsible for neurodegeneration, cognitive decline, and loss of memory in patients affected with Alzheimer’s disease. It is estimated that Alzheimer’s disease ranks third, just behind heart disease and cancer, as a cause of death for the elderly in the United States. AIRAP and AIRAPL are ubiquitin-binding proteins that are thought to enhance the function of the proteasome by clearing out the misfolded proteins from the brain. Previous studies have shown that AIP-1 (a homologue of AIRAP and AIRAPL) was overexpressed in a Caenorhabditis elegans disease model which in turn helped to reduce the accumulation of Aβ and thus had a protective effect against Aβ toxicity. The purpose of our study is to test whether AIRAP and AIRAPL are protective against the toxicity associated with Aβ in mammalian neuronal cells. We are hypothesizing that AIRAP and AIRAPL are protective against Aβ toxicity in HT22 hippocampal cells, which would significantly decelerate the progression of Alzheimer’s disease. We have manipulated the expression of AIRAP and AIRAPL and studied the effect of overexpression and knockdown on Aβ toxicity. Overexpression was achieved by establishing transfected cell lines overexpressing AIRAP and AIRAPL with help of a CMV constitutive promoter. On the other hand, knockdown of genes was attained using specific DsiRNA. Using Trypan Blue staining, we found that cell lines overexpressing AIRAP/AIRAPL could lower Aβ toxicity, thus increasing the number of viable cells. Additionally, knocking down AIRAP/AIRAPL expression appeared to worsen the condition of cells, as the number of dead cells increased, in response to Aβ treatment. With help of real-time PCR, mRNA levels of AIRAP and AIRAPL was measured. It was shown that expression of both AIRAP and AIRAPL was enhanced during the initial stages of induction in cell line having induced Aβ. However, as the induction time increased, the expression levels started to fall off. Further studies are essential to know the exact mechanism behind AIRAP/AIRAPL protection against Aβ toxicity. Advisors/Committee Members: Wail Hassan.

Subjects/Keywords: Medicine and Health Sciences

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Chakraborty, A. (2017). The Potential Role of AIRAP/AIRAPL in Ameliorating the Toxicity Related to Amyloid-beta (aβ) in Mammalian Neuronal Cells. (Thesis). University of Wisconsin – Milwaukee. Retrieved from https://dc.uwm.edu/etd/1593

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Chakraborty, Advaita. “The Potential Role of AIRAP/AIRAPL in Ameliorating the Toxicity Related to Amyloid-beta (aβ) in Mammalian Neuronal Cells.” 2017. Thesis, University of Wisconsin – Milwaukee. Accessed March 02, 2021. https://dc.uwm.edu/etd/1593.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Chakraborty, Advaita. “The Potential Role of AIRAP/AIRAPL in Ameliorating the Toxicity Related to Amyloid-beta (aβ) in Mammalian Neuronal Cells.” 2017. Web. 02 Mar 2021.

Vancouver:

Chakraborty A. The Potential Role of AIRAP/AIRAPL in Ameliorating the Toxicity Related to Amyloid-beta (aβ) in Mammalian Neuronal Cells. [Internet] [Thesis]. University of Wisconsin – Milwaukee; 2017. [cited 2021 Mar 02]. Available from: https://dc.uwm.edu/etd/1593.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Chakraborty A. The Potential Role of AIRAP/AIRAPL in Ameliorating the Toxicity Related to Amyloid-beta (aβ) in Mammalian Neuronal Cells. [Thesis]. University of Wisconsin – Milwaukee; 2017. Available from: https://dc.uwm.edu/etd/1593

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Purdue University

11. Klinkhamer, Christopher Jacob. The combined effects of anthropogenic and environmental stressors on Fundulus grandis development.

Degree: MS, Health Sciences, 2014, Purdue University

URL: http://docs.lib.purdue.edu/open_access_theses/642

► The 2010 Deepwater Horizon oil spill resulted in the release of ~ 5 million barrels of crude oil from the Macondo wellhead into the… (more)

Subjects/Keywords: Medicine and Health Sciences; Toxicology

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Klinkhamer, C. J. (2014). The combined effects of anthropogenic and environmental stressors on Fundulus grandis development. (Thesis). Purdue University. Retrieved from http://docs.lib.purdue.edu/open_access_theses/642

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Klinkhamer, Christopher Jacob. “The combined effects of anthropogenic and environmental stressors on Fundulus grandis development.” 2014. Thesis, Purdue University. Accessed March 02, 2021. http://docs.lib.purdue.edu/open_access_theses/642.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Klinkhamer, Christopher Jacob. “The combined effects of anthropogenic and environmental stressors on Fundulus grandis development.” 2014. Web. 02 Mar 2021.

Vancouver:

Klinkhamer CJ. The combined effects of anthropogenic and environmental stressors on Fundulus grandis development. [Internet] [Thesis]. Purdue University; 2014. [cited 2021 Mar 02]. Available from: http://docs.lib.purdue.edu/open_access_theses/642.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Klinkhamer CJ. The combined effects of anthropogenic and environmental stressors on Fundulus grandis development. [Thesis]. Purdue University; 2014. Available from: http://docs.lib.purdue.edu/open_access_theses/642

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Purdue University

12. Wright, Breanne N. Iron nutriture following roux-en-y gastric bypass surgery.

Degree: MS, Nutrition Science, 2014, Purdue University

URL: http://docs.lib.purdue.edu/open_access_theses/707

► Roux-en-Y gastric bypass surgery (RYGB) is effective for weight loss, but is commonly associated with iron deficiency and its clinical manifestation, anemia. Diagnosing iron… (more)

▼ Roux-en-Y gastric bypass surgery (RYGB) is effective for weight loss, but is commonly associated with iron deficiency and its clinical manifestation, anemia. Diagnosing iron deficiency is complex because iron status depends on other nutrients; additionally, anemia following surgery is not specifically due to deficiency in iron, as it can be due to deficiencies in other nutrients including zinc, copper, vitamin B₆, folate, and vitamin B₁₂. In patients who have undergone RYGB, our aims were to 1) conduct a comprehensive assessment of nutrients involved in iron homeostasis, 2) determine the contribution of dietary intake to iron deficiency, and 3) describe associations between anemia and nutritional status of iron and other nutrients. Systemic measures of hemoglobin, ferritin, serum transferrin receptor (sTfR), total iron binding capacity (TIBC), copper (Cu), vitamins B₆ and B₁₂, folate, zinc (Zn), and C–reactive protein (CRP) were determined using reference methods. Iron deficiency equaled having ≥ 2 abnormalities in: ferritin, sTfR, sTfR:ferritin, or TIBC. Ferritin, a measure of iron stores, was defined as normal (ferritin ≥ 20 mcg/L) or low (ferritin < 20 mcg/L). Statistics included prevalence, mean ± standard error of the mean (s.e.m.) for normally–distributed data, median ± semi-interquartile range for skewed data (indicated with an asterisk [*]), frequency tables, t–tests (independent, by group), correlations, and general linear models (significant if p < 0.05). Subjects (N=70) were 91% female, age 49 ± 1 years, *4 ± 2 years post surgery, and 79% Caucasian. Fifty–six percent of the total population and 96% of the subpopulation with anemia (N=26) presented with deficiencies related to iron nutriture, including deficiencies in iron, zinc, copper, vitamin B₆, folate, and vitamin B₁₂. The most prevalent nutrient deficiencies in the total population and the subpopulation with anemia were iron and zinc; prevalence of iron and zinc deficiency in the total population was 24.3% and 20.0%, respectively, and prevalence of iron and zinc deficiency in the subpopulation with anemia was 46.2% and 23.1%, respectively. Participants in the total population and the subpopulation with anemia were also deficient in copper, vitamin B₆, vitamin B₁₂, and folate (11.5% and 26.9%, respectively). In the total population, iron and zinc deficiency occurred in isolation and also in combination with other nutrient deficiencies; all other nutrient deficiencies occurred in combination. In the subpopulation with anemia, only iron deficiency occurred in isolation. The dietary intake of the study population exceeded the RDAs for all nutrients assessed. In addition, patients with low ferritin concentrations consumed lower total energy (p= 0.009), fat (p= 0.026), protein (p=0.013), and animal protein (p=0.023), compared to patients with normal ferritin concentrations. Dietary intake of heme iron was correlated with years post–RYGB surgery (r=0.67, p<0.05). In… Advisors/Committee Members: Nana A. Gletsu-Miller, Nana A. Gletsu-Miller, James C. Fleet, Dorothy Teegarden.

Subjects/Keywords: Medicine and Health Sciences; Nutrition

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Wright, B. N. (2014). Iron nutriture following roux-en-y gastric bypass surgery. (Thesis). Purdue University. Retrieved from http://docs.lib.purdue.edu/open_access_theses/707

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Wright, Breanne N. “Iron nutriture following roux-en-y gastric bypass surgery.” 2014. Thesis, Purdue University. Accessed March 02, 2021. http://docs.lib.purdue.edu/open_access_theses/707.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Wright, Breanne N. “Iron nutriture following roux-en-y gastric bypass surgery.” 2014. Web. 02 Mar 2021.

Vancouver:

Wright BN. Iron nutriture following roux-en-y gastric bypass surgery. [Internet] [Thesis]. Purdue University; 2014. [cited 2021 Mar 02]. Available from: http://docs.lib.purdue.edu/open_access_theses/707.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Wright BN. Iron nutriture following roux-en-y gastric bypass surgery. [Thesis]. Purdue University; 2014. Available from: http://docs.lib.purdue.edu/open_access_theses/707

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

13. Camacho-Walsh, Mercedes. Evidence-Based Practice| Reducing Unnecessary Antibiotic Prescriptions for Pediatric Pharyngitis.

Degree: 2018, Saint Peter's University

URL: http://pqdtopen.proquest.com/#viewpdf?dispub=10742646

► The purpose of this project was to decrease of the rate of unnecessary antibiotic prescribing for pharyngitis by implementing an evidence-based training session for… (more)

▼ The purpose of this project was to decrease of the rate of unnecessary antibiotic prescribing for pharyngitis by implementing an evidence-based training session for physicians in an outpatient pediatric setting. The PICOT question explored was, "For health providers treating children aged 4–15 presenting with sore throat, will the use of a power point training session presenting the rapid antigen detection test (RADT) with reflexive culture, combined with the ICE (ideas, concern and expectations) method, improve knowledge and reduce antibiotic prescribing compared to RADT alone in a 20 day period?" The provider study group consisted of four pediatricians and one family practice physician ranging from 32–72 years old. Their pre-test (34.63%) and post-test (53.75%) knowledge scores were significantly different ( t = –2.3822, df = 6, p < 0.05). A total of 125 cases were sampled, 64 pre-intervention and 61 post-intervention. Pearson’s Chi Square analysis revealed homogeneity between both the groups in age (X² = 0.94, df = 1, p = 0.33), gender (X2 = 0.64, df = 1, p = 0.42), and ethnicity (X2 = 1.29, df = 2, p = 0.53) and a decrease in overall antibiotic prescribing rates from 40.6% (n = 26) to 27.9% (n = 17). Although this was not a significant statistical reduction (p = .13), further analysis using a binomial test revealed statistically different rates of success in the accuracy of diagnosis and associated antibiotic prescribing pre-intervention (79.7%) compared to 96.7% post-intervention (p = .00; 95% CI [88.7, 99.6]). Unnecessary antibiotic exposure was reduced by 17.2%. The most common ICE elements were thought of possible strep infection (39), viral or other infection (26), concern for pain (24), infecting other family members (14), fever (14), expectation to get better (32), test for strep (18), and pain relief (9). Only 2 of the 5 cases in the post-intervention group (n = 61) who expressed desire for antibiotics received them.

Subjects/Keywords: Medicine; Health sciences; Nursing

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APA (6^th Edition):

Camacho-Walsh, M. (2018). Evidence-Based Practice| Reducing Unnecessary Antibiotic Prescriptions for Pediatric Pharyngitis. (Thesis). Saint Peter's University. Retrieved from http://pqdtopen.proquest.com/#viewpdf?dispub=10742646

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Camacho-Walsh, Mercedes. “Evidence-Based Practice| Reducing Unnecessary Antibiotic Prescriptions for Pediatric Pharyngitis.” 2018. Thesis, Saint Peter's University. Accessed March 02, 2021. http://pqdtopen.proquest.com/#viewpdf?dispub=10742646.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Camacho-Walsh, Mercedes. “Evidence-Based Practice| Reducing Unnecessary Antibiotic Prescriptions for Pediatric Pharyngitis.” 2018. Web. 02 Mar 2021.

Vancouver:

Camacho-Walsh M. Evidence-Based Practice| Reducing Unnecessary Antibiotic Prescriptions for Pediatric Pharyngitis. [Internet] [Thesis]. Saint Peter's University; 2018. [cited 2021 Mar 02]. Available from: http://pqdtopen.proquest.com/#viewpdf?dispub=10742646.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Camacho-Walsh M. Evidence-Based Practice| Reducing Unnecessary Antibiotic Prescriptions for Pediatric Pharyngitis. [Thesis]. Saint Peter's University; 2018. Available from: http://pqdtopen.proquest.com/#viewpdf?dispub=10742646

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Virginia Commonwealth University

14. Chalasani, Sri Lakshmi. TOLERANCE OF THYMINE GLYCOL AT THE BLUNT DNA DOUBLE STRAND BREAK FOR NONHOMOLOGUS END JOINING REPAIR AND INTERFERENCE BY BASE EXCISION REPAIR.

Degree: MS, Pharmacology & Toxicology, 2015, Virginia Commonwealth University

URL: https://doi.org/10.25772/MKF5-MY97 ; https://scholarscompass.vcu.edu/etd/3921

► Radiotherapy is the clinical application of the ionizing radiation to treat cancer. Ionizing radiation causes multiple modes of damage to the DNA damage such… (more)

▼ Radiotherapy is the clinical application of the ionizing radiation to treat cancer. Ionizing radiation causes multiple modes of damage to the DNA damage such as SSBs, DSBs and modified bases such as thymine glycol. These lesions can exist as clusters in one or two helical turns of DNA. DNA double-strand breaks (DSBs) are extremely toxic to cells because they can lead to genomic rearrangements and even cell death. If base lesions accompany these DSBs, there will be a substantial hindrance for repair. NHEJ is the primary DSB repair pathway in mammalian cells. HRR repairs single strand breaks (SSBs) or Double strand breaks (DSBs), during late S phase and G2 phase of the cell cycle, by using an undamaged copy of the DNA sequence, and is therefore largely error-free. The NHEJ pathway repairs DSBs without the requirement for sequence homology and can be error-free or error-prone, and is most active during G1 phase. Thymine glycol (Tg) is the most common oxidation product of thymine. It is produced endogenously as a consequence of aerobic metabolism or via exogenous factors such as ionizing radiation (IR); it is one of the predominant types of base modifications produced by ionizing radiation. Due to clustering of radiation-induced damages, many DSBs are accompanied by damaged bases such as Tg at or near the DSB ends that may interfere with subsequent gap filling and ligation. The base excision repair pathway plays a major role in removal of thymine glycol from the damaged DNA strand. During NHEJ, after synapsis by Ku and DNAPKcs and processing of the DNA ends, XRCC4/Ligase IV complex ligates the DNA. This ligase activity is promoted by the interaction of XLF/XRCC4 filament with Ligase IV. Linearized plasmids with Tg at the 5th -Tg5 positions from the broken end were subjected to a repair assay using XRCC4-like factor (XLF)-deficient cell extracts, with or without the addition of XLF and Endonuclease III and/or ddTTP and Klenow fragments. End joining of Tg5 was compared to plasmid with Tg at third position-Tg3 in extracts. In addition, the ability of purified NHEJ proteins Ku, DNAPKcs and XRCC4/Ligase IV, to repair the Tg1 (Tg at the end), Tg2 (Tg at the second position), Tg3 and Tg5 in the presence and absence of XLF was assessed. The data indicated that the cell extract could ligate the Tg5 plasmids only in the presence of XLF. End joining of the Tg5 was less in comparison to Tg3 with base excision repair being more active in Tg5 competing with the joining. Plasmids with Tg were treated with Endonuclease III and ddTTP to test whether the end joining occurred before or after Tg removal. Endonuclease III and ddTTP treatment showed reduced intensity of the joined fragment suggesting that end joining occurred without removal of Tg in some cases. The extracts were not able to fill in the processed end by the BER though it has a 3′-OH which was filled in by the treatment with Klenow enzymes derived from E.coli DNA Polymerase I, following removal of extract proteins by… Advisors/Committee Members: Lawrence F Povirk.

Subjects/Keywords: Medicine and Health Sciences

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Chalasani, S. L. (2015). TOLERANCE OF THYMINE GLYCOL AT THE BLUNT DNA DOUBLE STRAND BREAK FOR NONHOMOLOGUS END JOINING REPAIR AND INTERFERENCE BY BASE EXCISION REPAIR. (Thesis). Virginia Commonwealth University. Retrieved from https://doi.org/10.25772/MKF5-MY97 ; https://scholarscompass.vcu.edu/etd/3921

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Chalasani, Sri Lakshmi. “TOLERANCE OF THYMINE GLYCOL AT THE BLUNT DNA DOUBLE STRAND BREAK FOR NONHOMOLOGUS END JOINING REPAIR AND INTERFERENCE BY BASE EXCISION REPAIR.” 2015. Thesis, Virginia Commonwealth University. Accessed March 02, 2021. https://doi.org/10.25772/MKF5-MY97 ; https://scholarscompass.vcu.edu/etd/3921.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Chalasani, Sri Lakshmi. “TOLERANCE OF THYMINE GLYCOL AT THE BLUNT DNA DOUBLE STRAND BREAK FOR NONHOMOLOGUS END JOINING REPAIR AND INTERFERENCE BY BASE EXCISION REPAIR.” 2015. Web. 02 Mar 2021.

Vancouver:

Chalasani SL. TOLERANCE OF THYMINE GLYCOL AT THE BLUNT DNA DOUBLE STRAND BREAK FOR NONHOMOLOGUS END JOINING REPAIR AND INTERFERENCE BY BASE EXCISION REPAIR. [Internet] [Thesis]. Virginia Commonwealth University; 2015. [cited 2021 Mar 02]. Available from: https://doi.org/10.25772/MKF5-MY97 ; https://scholarscompass.vcu.edu/etd/3921.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Chalasani SL. TOLERANCE OF THYMINE GLYCOL AT THE BLUNT DNA DOUBLE STRAND BREAK FOR NONHOMOLOGUS END JOINING REPAIR AND INTERFERENCE BY BASE EXCISION REPAIR. [Thesis]. Virginia Commonwealth University; 2015. Available from: https://doi.org/10.25772/MKF5-MY97 ; https://scholarscompass.vcu.edu/etd/3921

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Virginia Commonwealth University

15. Damle, Priyadarshan. Helper phage capsid size redirection by staphylococcal pathogenicity island SaPI1 involves internal scaffolding proteins.

Degree: PhD, Microbiology & Immunology, 2011, Virginia Commonwealth University

URL: https://doi.org/10.25772/Y771-YT29 ; https://scholarscompass.vcu.edu/etd/255

► Staphylococcus aureus is one of the leading causes of nosocomial and community acquired infections. Many of the virulence factors are encoded on staphylococcal mobile genetic… (more)

Subjects/Keywords: Medicine and Health Sciences

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Damle, P. (2011). Helper phage capsid size redirection by staphylococcal pathogenicity island SaPI1 involves internal scaffolding proteins. (Doctoral Dissertation). Virginia Commonwealth University. Retrieved from https://doi.org/10.25772/Y771-YT29 ; https://scholarscompass.vcu.edu/etd/255

Chicago Manual of Style (16^th Edition):

Damle, Priyadarshan. “Helper phage capsid size redirection by staphylococcal pathogenicity island SaPI1 involves internal scaffolding proteins.” 2011. Doctoral Dissertation, Virginia Commonwealth University. Accessed March 02, 2021. https://doi.org/10.25772/Y771-YT29 ; https://scholarscompass.vcu.edu/etd/255.

MLA Handbook (7^th Edition):

Damle, Priyadarshan. “Helper phage capsid size redirection by staphylococcal pathogenicity island SaPI1 involves internal scaffolding proteins.” 2011. Web. 02 Mar 2021.

Vancouver:

Damle P. Helper phage capsid size redirection by staphylococcal pathogenicity island SaPI1 involves internal scaffolding proteins. [Internet] [Doctoral dissertation]. Virginia Commonwealth University; 2011. [cited 2021 Mar 02]. Available from: https://doi.org/10.25772/Y771-YT29 ; https://scholarscompass.vcu.edu/etd/255.

Council of Science Editors:

Damle P. Helper phage capsid size redirection by staphylococcal pathogenicity island SaPI1 involves internal scaffolding proteins. [Doctoral Dissertation]. Virginia Commonwealth University; 2011. Available from: https://doi.org/10.25772/Y771-YT29 ; https://scholarscompass.vcu.edu/etd/255

Virginia Commonwealth University

16. Mallory, Katherine Louise. Characterization of cyclic di-GMP binding by the sole Borrelia burgdorferi and Borrelia hermsii PilZ domain-containing proteins.

Degree: MS, Microbiology & Immunology, 2013, Virginia Commonwealth University

URL: https://doi.org/10.25772/XXQW-NX76 ; https://scholarscompass.vcu.edu/etd/532

► Borrelia burgdorferi and Borrelia hermsii cause Lyme disease and relapsing fever, respectively. These spirochetes are maintained in an enzootic cycle, involving tick vectors and mammalian… (more)

Subjects/Keywords: Medicine and Health Sciences

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APA (6^th Edition):

Mallory, K. L. (2013). Characterization of cyclic di-GMP binding by the sole Borrelia burgdorferi and Borrelia hermsii PilZ domain-containing proteins. (Thesis). Virginia Commonwealth University. Retrieved from https://doi.org/10.25772/XXQW-NX76 ; https://scholarscompass.vcu.edu/etd/532

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Mallory, Katherine Louise. “Characterization of cyclic di-GMP binding by the sole Borrelia burgdorferi and Borrelia hermsii PilZ domain-containing proteins.” 2013. Thesis, Virginia Commonwealth University. Accessed March 02, 2021. https://doi.org/10.25772/XXQW-NX76 ; https://scholarscompass.vcu.edu/etd/532.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Mallory, Katherine Louise. “Characterization of cyclic di-GMP binding by the sole Borrelia burgdorferi and Borrelia hermsii PilZ domain-containing proteins.” 2013. Web. 02 Mar 2021.

Vancouver:

Mallory KL. Characterization of cyclic di-GMP binding by the sole Borrelia burgdorferi and Borrelia hermsii PilZ domain-containing proteins. [Internet] [Thesis]. Virginia Commonwealth University; 2013. [cited 2021 Mar 02]. Available from: https://doi.org/10.25772/XXQW-NX76 ; https://scholarscompass.vcu.edu/etd/532.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Mallory KL. Characterization of cyclic di-GMP binding by the sole Borrelia burgdorferi and Borrelia hermsii PilZ domain-containing proteins. [Thesis]. Virginia Commonwealth University; 2013. Available from: https://doi.org/10.25772/XXQW-NX76 ; https://scholarscompass.vcu.edu/etd/532

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Virginia Commonwealth University

17. Maddux, Rebecca. CANNABINOIDS DELTA-9-TETRAHYDROCANNABINOL (THC) AND CP55,940 ABLATE SPECIFIC CHEMOKINE AND CYTOKINE INFLAMMATORY RESPONSE IN BV-2 MOUSE MICROGLIAL CELLS ACTIVATED WITH HIV-1 PRO-INFLAMMATORY PROTEIN TAT.

Degree: MS, Microbiology & Immunology, 2013, Virginia Commonwealth University

URL: https://doi.org/10.25772/CAN6-T857 ; https://scholarscompass.vcu.edu/etd/545

► Despite the widespread use of Highly Active Anti-retroviral Therapy (HAART) to combat Human Immunodeficiency Virus (HIV), the causative agent of acquired immunodeficiency syndrome (AIDS), HIV-Associated… (more)

Subjects/Keywords: Medicine and Health Sciences

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Maddux, R. (2013). CANNABINOIDS DELTA-9-TETRAHYDROCANNABINOL (THC) AND CP55,940 ABLATE SPECIFIC CHEMOKINE AND CYTOKINE INFLAMMATORY RESPONSE IN BV-2 MOUSE MICROGLIAL CELLS ACTIVATED WITH HIV-1 PRO-INFLAMMATORY PROTEIN TAT. (Thesis). Virginia Commonwealth University. Retrieved from https://doi.org/10.25772/CAN6-T857 ; https://scholarscompass.vcu.edu/etd/545

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Maddux, Rebecca. “CANNABINOIDS DELTA-9-TETRAHYDROCANNABINOL (THC) AND CP55,940 ABLATE SPECIFIC CHEMOKINE AND CYTOKINE INFLAMMATORY RESPONSE IN BV-2 MOUSE MICROGLIAL CELLS ACTIVATED WITH HIV-1 PRO-INFLAMMATORY PROTEIN TAT.” 2013. Thesis, Virginia Commonwealth University. Accessed March 02, 2021. https://doi.org/10.25772/CAN6-T857 ; https://scholarscompass.vcu.edu/etd/545.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Maddux, Rebecca. “CANNABINOIDS DELTA-9-TETRAHYDROCANNABINOL (THC) AND CP55,940 ABLATE SPECIFIC CHEMOKINE AND CYTOKINE INFLAMMATORY RESPONSE IN BV-2 MOUSE MICROGLIAL CELLS ACTIVATED WITH HIV-1 PRO-INFLAMMATORY PROTEIN TAT.” 2013. Web. 02 Mar 2021.

Vancouver:

Maddux R. CANNABINOIDS DELTA-9-TETRAHYDROCANNABINOL (THC) AND CP55,940 ABLATE SPECIFIC CHEMOKINE AND CYTOKINE INFLAMMATORY RESPONSE IN BV-2 MOUSE MICROGLIAL CELLS ACTIVATED WITH HIV-1 PRO-INFLAMMATORY PROTEIN TAT. [Internet] [Thesis]. Virginia Commonwealth University; 2013. [cited 2021 Mar 02]. Available from: https://doi.org/10.25772/CAN6-T857 ; https://scholarscompass.vcu.edu/etd/545.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Maddux R. CANNABINOIDS DELTA-9-TETRAHYDROCANNABINOL (THC) AND CP55,940 ABLATE SPECIFIC CHEMOKINE AND CYTOKINE INFLAMMATORY RESPONSE IN BV-2 MOUSE MICROGLIAL CELLS ACTIVATED WITH HIV-1 PRO-INFLAMMATORY PROTEIN TAT. [Thesis]. Virginia Commonwealth University; 2013. Available from: https://doi.org/10.25772/CAN6-T857 ; https://scholarscompass.vcu.edu/etd/545

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Virginia Commonwealth University

18. Gade, Aravind. THE ROLE OF α3β4* SUBTYPE OF NICOTINIC ACETYLCHOLINE RECEPTORS IN REVERSING OPIOID-INDUCED CONSTIPATION IN MICE.

Degree: PhD, Pharmacology & Toxicology, 2015, Virginia Commonwealth University

URL: https://doi.org/10.25772/BQJG-D471 ; https://scholarscompass.vcu.edu/etd/4037

► Opioids are excellent pain relievers. A major side-effect of chronic opioid treatment is constipation whereas withdrawal following chronic exposure leads to diarrhea and increased… (more)

▼ Opioids are excellent pain relievers. A major side-effect of chronic opioid treatment is constipation whereas withdrawal following chronic exposure leads to diarrhea and increased gastrointestinal motility. These effects of chronic opioids are mediated by μ-opioid receptors expressed on enteric neurons. Previous studies have shown that chronic opioids enhance sensitivity to nicotine in the gastrointestinal tract. This suggested that prokinetic effects of nicotine mediated through the activation of nicotinic acetylcholine receptors (nAChRs) may be useful in reversing opioid-induced constipation. The goal of this dissertation was to investigate the nAChR subtype expressed on enteric neurons and their role in reversing opioid-induced constipation. The effect of nicotine on small intestinal transit and fecal pellet output were determined in-vivo in morphine-pelleted mice xiii (75 mg for 4 days). Nicotine-induced currents were measured by whole-cell voltage clamp in isolated adult mouse myenteric neurons treated with morphine over short term (10 mins) and long term (16-20 hrs). Following long term morphine exposure in-vivo (morphine pellet – 4 days), nicotine increased fecal pellet output, and enhanced small intestinal transit. The prokinetic effect of nicotine was not seen in placebo pelleted mice or after acute morphine (10 mg/kg, 30 min). Peak-amplitude of nicotine-induced inward currents in isolated neurons was also enhanced after long-term but not short term exposure to morphine. Nicotine-induced currents were inhibited by mecamylamine (10 μM) and α-conotoxin AUIB (3 μM), suggesting the expression of α3β4 subtype of nAChRs on enteric neurons. Conversely, NS3861, a partial agonist at α3β4 nAChR enhanced fecal pellet expulsion in a dose-dependent manner in chronic but not acute morphine treated mice. Overall, our findings suggest that the efficacy of nAChR agonists on enteric neurons is enhanced after chronic morphine exposure and activation of α3β4 subtype of nAChR reverses chronic but not acute morphine induced constipation. In conclusion, development of peripherally selective α3β4 partial agonists may be of therapeutic benefit in treatment of chronic opioid-induced constipation. Advisors/Committee Members: Hamid I. Akbarali.

Subjects/Keywords: Medicine and Health Sciences

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Gade, A. (2015). THE ROLE OF α3β4* SUBTYPE OF NICOTINIC ACETYLCHOLINE RECEPTORS IN REVERSING OPIOID-INDUCED CONSTIPATION IN MICE. (Doctoral Dissertation). Virginia Commonwealth University. Retrieved from https://doi.org/10.25772/BQJG-D471 ; https://scholarscompass.vcu.edu/etd/4037

Chicago Manual of Style (16^th Edition):

Gade, Aravind. “THE ROLE OF α3β4* SUBTYPE OF NICOTINIC ACETYLCHOLINE RECEPTORS IN REVERSING OPIOID-INDUCED CONSTIPATION IN MICE.” 2015. Doctoral Dissertation, Virginia Commonwealth University. Accessed March 02, 2021. https://doi.org/10.25772/BQJG-D471 ; https://scholarscompass.vcu.edu/etd/4037.

MLA Handbook (7^th Edition):

Gade, Aravind. “THE ROLE OF α3β4* SUBTYPE OF NICOTINIC ACETYLCHOLINE RECEPTORS IN REVERSING OPIOID-INDUCED CONSTIPATION IN MICE.” 2015. Web. 02 Mar 2021.

Vancouver:

Gade A. THE ROLE OF α3β4* SUBTYPE OF NICOTINIC ACETYLCHOLINE RECEPTORS IN REVERSING OPIOID-INDUCED CONSTIPATION IN MICE. [Internet] [Doctoral dissertation]. Virginia Commonwealth University; 2015. [cited 2021 Mar 02]. Available from: https://doi.org/10.25772/BQJG-D471 ; https://scholarscompass.vcu.edu/etd/4037.

Council of Science Editors:

Gade A. THE ROLE OF α3β4* SUBTYPE OF NICOTINIC ACETYLCHOLINE RECEPTORS IN REVERSING OPIOID-INDUCED CONSTIPATION IN MICE. [Doctoral Dissertation]. Virginia Commonwealth University; 2015. Available from: https://doi.org/10.25772/BQJG-D471 ; https://scholarscompass.vcu.edu/etd/4037

Wayne State University

19. Wang, Jihan. Operating room utilization and turnover behavioral study.

Degree: PhD, Industrial and Manufacturing Engineering, 2012, Wayne State University

URL: https://digitalcommons.wayne.edu/oa_dissertations/559

► Operating Rooms (OR) are the most expensive care units in health care systems. In order for OR theatre to operate in cost efficient way,… (more)

▼ Operating Rooms (OR) are the most expensive care units in health care systems. In order for OR theatre to operate in cost efficient way, it is desirable that the ORs exhibit high utilization, while at the same time, maintain a low-level over-utilized OR time. At the operational level, there are many factors that could influence the OR utilization performances. The objective of this study is to develop effective approaches focusing on the most important factors that influence OR utilization to assist OR management in decision making to achieve better utilization and cost efficiency. In the study, model selection and cross-validation methods were used to find the best linear model of OR utilization given different subsets of the factors. As the scheduled utilization and case duration prediction accuracy were identified as the two most statistically significant factors, we then proposed a new distribution to approximate the total duration of surgery lists of multiple cases and compared its accuracy in the estimation of the probability of under- and over-run of surgery lists with the widely applied t-distribution. Monte Carlo simulation was used to validate the appropriateness of the proposed new distribution by comparing the percentiles of the empirical distribution of the duration of surgery lists with those calculated from the proposed distribution. The tardiness of case starts prohibit OR from achieving optimum efficiency as they causes over-utilized OR time and cancellations. Given limited resources, it is critical for the OR management to prioritize the tackling of tardiness. An iterative simulation method considering multiple delay reasons at a time was proposed that continuously identifies the top delay risks to facilitate proactive decision making to prevent tardiness from taking place. The effectiveness of this approach was examined through a case study by having different scheduling policies and case duration distributions. In the end, the behavioral pattern of OR staff was explored by constructing a structural equation model. Relationships among different variables and mean turnover time duration were estimated. It was found out that the work pace of OR staff during turnover times was not affected by the OR workload, and proved there was a psychological bias of OR staff to make decisions based on increasing clinical work per unit time during the hours they are assigned. This research complements current OR management study by introducing better and new methods for OR operational decision making. Advisors/Committee Members: Kai Yang.

Subjects/Keywords: Medicine and Health Sciences

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APA (6^th Edition):

Wang, J. (2012). Operating room utilization and turnover behavioral study. (Doctoral Dissertation). Wayne State University. Retrieved from https://digitalcommons.wayne.edu/oa_dissertations/559

Chicago Manual of Style (16^th Edition):

Wang, Jihan. “Operating room utilization and turnover behavioral study.” 2012. Doctoral Dissertation, Wayne State University. Accessed March 02, 2021. https://digitalcommons.wayne.edu/oa_dissertations/559.

MLA Handbook (7^th Edition):

Wang, Jihan. “Operating room utilization and turnover behavioral study.” 2012. Web. 02 Mar 2021.

Vancouver:

Wang J. Operating room utilization and turnover behavioral study. [Internet] [Doctoral dissertation]. Wayne State University; 2012. [cited 2021 Mar 02]. Available from: https://digitalcommons.wayne.edu/oa_dissertations/559.

Council of Science Editors:

Wang J. Operating room utilization and turnover behavioral study. [Doctoral Dissertation]. Wayne State University; 2012. Available from: https://digitalcommons.wayne.edu/oa_dissertations/559

Wayne State University

20. Saini, Jatinder. Commissioning And Validation Of Analytical And Monte Carlo Based Dose Calculation Algorithms For Proton Spot Scanning.

Degree: PhD, Medical Physics, 2017, Wayne State University

URL: https://digitalcommons.wayne.edu/oa_dissertations/1867

► Purpose: Accurate dose calculation is one of the most necessary components of radiation therapy. While the commercially available photon dose calculation algorithm offerings have… (more)

▼ Purpose: Accurate dose calculation is one of the most necessary components of radiation therapy. While the commercially available photon dose calculation algorithm offerings have improved considerably in last decade, proton dose calculations are still performed using the analytical dose calculation algorithms. The goal of this work is to validate a newly available commercial Monte Carlo (MC) dose calculation algorithm using measurements and simulations in GATE software. A secondary goal is to compare and contrast the performance of analytical algorithm against MC algorithm. Finally, GATE simulations are used to evaluate a newly available ceramic marker for ocular melanoma proton therapy. Methods: An analytical and MC beam models of a full 360 degree gantry at SCCA proton therapy center were commissioned in RayStation treatment planning system. Measurements were performed using variety of detectors such as parallel plate ion chambers, 2D ion chamber arrays, Bragg peaks chamber, 2D high resolution scintillation imager, and radiographic film. The analytical beam model was put to a series of tests that involved verification of point doses, PDDs, profiles, and doses in patient specific plans. The comparison of analytical and RayStation MC (RS-MC) algorithm was carried out by measurements in homogenous, heterogeneous, and anthropomorphic phantoms. For comparisons against simulations, a beam model was developed in GATE MC Toolkit using the measured beam data. For evaluation of ceramic marker, a custom phantom with styrofoam insert with embedded marker was created. Simulation and measurements were made for marker with clip in parallel, perpendicular and transverse orientation relative to beam. Results: For the analytical algorithm, evaluation of point doses in water showed dose differences>3% for proton ranges>30 cm, field sizes>15 x15 cm2, and depths>25 cm. When a range shifter was employed, analytical algorithm showed dose up to 10% dose difference in the entrance region for air gaps>30 cm. In oblique beam conditions, analytical algorithm showed broadening in distal penumbra by up to 5 mm. RS-MC algorithm matched measurements and GATE simulations to within 3% at all points for SOBP depth doses, beam with a range shifter, and oblique incidence. RS-MC also predicted accurate doses in inhomogeneous phantom, where the dose profile created at the interface matched to measurements and simulations with 100% gamma index (GI) pass rate at 3% dose and 3 mm distance-to-agreement (DTA). In anthropomorphic phantom, 6/7 planes had GI> 90% using 3% dose and 3 mm DTA for RS-MC. Corresponding numbers for analytical algorithm showed only 3/7 planes with GI >90%. The ceramic marker showed considerable dose attenuation behind the marker that worsened when marker was placed close to the distal edge. The transverse marker orientation in the clinical SOBP beam showed dose reduction of up to 61% and range pull-back of 2.4 mm. Conclusions: The RS-MC algorithm demonstrated improved dosimetric… Advisors/Committee Members: Charles D. Bloch, Jacob Burmeister.

Subjects/Keywords: Medicine and Health Sciences

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Saini, J. (2017). Commissioning And Validation Of Analytical And Monte Carlo Based Dose Calculation Algorithms For Proton Spot Scanning. (Doctoral Dissertation). Wayne State University. Retrieved from https://digitalcommons.wayne.edu/oa_dissertations/1867

Chicago Manual of Style (16^th Edition):

Saini, Jatinder. “Commissioning And Validation Of Analytical And Monte Carlo Based Dose Calculation Algorithms For Proton Spot Scanning.” 2017. Doctoral Dissertation, Wayne State University. Accessed March 02, 2021. https://digitalcommons.wayne.edu/oa_dissertations/1867.

MLA Handbook (7^th Edition):

Saini, Jatinder. “Commissioning And Validation Of Analytical And Monte Carlo Based Dose Calculation Algorithms For Proton Spot Scanning.” 2017. Web. 02 Mar 2021.

Vancouver:

Saini J. Commissioning And Validation Of Analytical And Monte Carlo Based Dose Calculation Algorithms For Proton Spot Scanning. [Internet] [Doctoral dissertation]. Wayne State University; 2017. [cited 2021 Mar 02]. Available from: https://digitalcommons.wayne.edu/oa_dissertations/1867.

Council of Science Editors:

Saini J. Commissioning And Validation Of Analytical And Monte Carlo Based Dose Calculation Algorithms For Proton Spot Scanning. [Doctoral Dissertation]. Wayne State University; 2017. Available from: https://digitalcommons.wayne.edu/oa_dissertations/1867

Virginia Commonwealth University

21. Farah, Christine. The Fluoride Recharging Capability of Orthodontic Materials: an in-vitro study.

Degree: MS, Dentistry, 2012, Virginia Commonwealth University

URL: https://doi.org/10.25772/WRJG-NS03 ; https://scholarscompass.vcu.edu/etd/2682

► Enamel demineralization in the form of white spot lesions (WSLs) around fixed orthodontic appliances is a persistent problem in patients with poor oral hygiene.These lesions… (more)

▼ Enamel demineralization in the form of white spot lesions (WSLs) around fixed orthodontic appliances is a persistent problem in patients with poor oral hygiene.These lesions can form rapidly within 4 weeks of bracket placement.The purpose of this in-vitro study was to investigate the fluoride recharging capability ofa commercially available orthodontic primer used to minimize the development of WSLs in patients. The three groups tested were: OpalSeal (n=20, Ultradent, South Jordan, UT), ProSeal (n=20, Reliance, Itasca, IL) and Transbond XT (control, n=10, 3M Unitek, Monrovia, CA). The samples(5mmin diameter x 1mm in thickness) weresuspended individually in vials filled with 10mL of deionized water usinga fishing line. The baseline fluoride ion release from all of the samples was measured after two weeks of changing the solution every other day. The samples were then randomly divided into two groups, toothbrush or gel. The samples in the toothbrush group were brushed for one minute every day for 7d, with fluoride containing toothpaste (Colgate-Palmolive Company, New York, NY) and placed in a new solution after each brushing. After 7d of brushing the fluoride ion release was measured. The samples in the gel group were immersed in 10mL of acidulated phosphoric fluoride gel (APF) for one minute, following manufacturer’s instructions, and then placed in a new vial with 10mL of deionized water. At the end of 24hrs fluoride ion release measurementswere made and the samples were placed individually in a new solution. The solution was changed weekly in the gel group over six weeks to simulate the typical length of time between two orthodontic appointments. A final fluoride ion release measurement was taken of all the discs in the gel group 6 weeks after the fluoride gel treatment. The results of repeated-measures analysis indicated that there were no significant differences between the groups at baseline and after 7d of toothbrushing time points. Opal Seal exhibited a significant increase in fluoride uptake (1.0ppm) after 24hrs of fluoride gel exposure but these levels gradually decreasedover 6 weeks (0.04ppm). Pro Seal and Transbond showed no significant fluoride release after the gel or toothpaste applications. The fluoride-containing primer, Opal Seal, had the ability to be recharged with fluoride ions from APF gel. However, the amount of fluoride released from recharged discs decreased gradually over a 6 weeks of time. Advisors/Committee Members: Eser Tufekci.

Subjects/Keywords: Dentistry; Medicine and Health Sciences

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Farah, C. (2012). The Fluoride Recharging Capability of Orthodontic Materials: an in-vitro study. (Thesis). Virginia Commonwealth University. Retrieved from https://doi.org/10.25772/WRJG-NS03 ; https://scholarscompass.vcu.edu/etd/2682

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Farah, Christine. “The Fluoride Recharging Capability of Orthodontic Materials: an in-vitro study.” 2012. Thesis, Virginia Commonwealth University. Accessed March 02, 2021. https://doi.org/10.25772/WRJG-NS03 ; https://scholarscompass.vcu.edu/etd/2682.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Farah, Christine. “The Fluoride Recharging Capability of Orthodontic Materials: an in-vitro study.” 2012. Web. 02 Mar 2021.

Vancouver:

Farah C. The Fluoride Recharging Capability of Orthodontic Materials: an in-vitro study. [Internet] [Thesis]. Virginia Commonwealth University; 2012. [cited 2021 Mar 02]. Available from: https://doi.org/10.25772/WRJG-NS03 ; https://scholarscompass.vcu.edu/etd/2682.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Farah C. The Fluoride Recharging Capability of Orthodontic Materials: an in-vitro study. [Thesis]. Virginia Commonwealth University; 2012. Available from: https://doi.org/10.25772/WRJG-NS03 ; https://scholarscompass.vcu.edu/etd/2682

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Virginia Commonwealth University

22. Balinang, Joyce. The Regulation of Mitochondrial DNMT1 During Oxidative Stress.

Degree: MS, Microbiology & Immunology, 2012, Virginia Commonwealth University

URL: https://doi.org/10.25772/4ZS0-8P80 ; https://scholarscompass.vcu.edu/etd/2826

► Epigenetics is the study of heritable gene expression due to alterations in the DNA structure other than the underlying DNA sequence. DNA methylation is one… (more)

▼ Epigenetics is the study of heritable gene expression due to alterations in the DNA structure other than the underlying DNA sequence. DNA methylation is one of the three types of epigenetic modifications found in the eukaryotic system. It involves the incorporation of a methyl group at the 5-position of cytosine residues in the DNA. DNA methylation is associated with several notorious disorders and diseases including Fragile X Syndrome, neurodegenerative disease (Parkinson’s, Alzhiemer, etc), diabetes and cancer. Cytosine methylation of mitochondrial DNA (mtDNA) was first demonstrated several decades ago but the mechanism of generating cytosine modification and its functional importance remain elusive. Our laboratory recently demonstrated that the enzyme involved in cytosine modification of mtDNA is a novel mitochondrial isoform of DNA Methyltransferase 1, mtDNMT1. This protein is encoded in the nucleus and targeted to the mitochondria via a N-terminal targeting sequence. Bioinformatic analysis of the DNMT1 coding sequence showed a consensus NRF1 binding site that coincidently overlaps a p53 binding site within the promoter region, previously shown by this group to repress DNMT1 expression. Previous studies in the Taylor laboratory showed that mtDNMT protein expression was regulated by the transcription factor NRF1 as well as its coactivator PGC1α. PGC1α and NRF1 stimulate a large body of genes that are involved in mitochondrial biogenesis and cellular respiration in response to environmental stress. Considering the previous findings in our laboratory regarding mtDNMT1 regulation and the importance of PGC1α and NRF1 in oxidative homeostasis, we asked whether there is a mitochondrial epigenetic component in the cell’s response to cellular stress and whether up-regulation of mtDNMT1 might be part of the general response to this stress. To investigate the relationship between mtDNA methylation and oxidative homeostasis we examined the regulation of mtDNMT1 by transcription factors that respond to oxidative stress. Conditions that induced oxidative stress were applied to HCT 116 and SH-SY5Y cell lines and the protein expression of DNMT1 was observed. Ethanol and hypoxia- induced oxidative stress were observed to increase to protein level of mtDNMT1 while total DNMT1 level either remained constant or decreased. The protein level of PGC1α and NRF1 remained low in HCT 116 cells exposed to hypoxic stress, despite elevated mtDNMT1 protein level. ChIP analysis of HCT 116 cells exposed to hypoxic stress demonstrated that NRF1 and PGC1α are not regulating the transcription of DNMT1i in the mitochondria. However, we observed that p53 dissociated from the DNMT1 promoter upon hypoxic stress, indicating that the up-regulation of mtDNMT1 is through the relief of p53 suppression. The findings of this investigation proved that mtDNMT1 is receptive to oxidative stress through the regulation by p53 and suggested that mitochondrial epigenetics may be playing an integral role in the cellular stress response toward hypoxia. Advisors/Committee Members: Shirley Taylor.

Subjects/Keywords: Medicine and Health Sciences

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Balinang, J. (2012). The Regulation of Mitochondrial DNMT1 During Oxidative Stress. (Thesis). Virginia Commonwealth University. Retrieved from https://doi.org/10.25772/4ZS0-8P80 ; https://scholarscompass.vcu.edu/etd/2826

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Balinang, Joyce. “The Regulation of Mitochondrial DNMT1 During Oxidative Stress.” 2012. Thesis, Virginia Commonwealth University. Accessed March 02, 2021. https://doi.org/10.25772/4ZS0-8P80 ; https://scholarscompass.vcu.edu/etd/2826.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Balinang, Joyce. “The Regulation of Mitochondrial DNMT1 During Oxidative Stress.” 2012. Web. 02 Mar 2021.

Vancouver:

Balinang J. The Regulation of Mitochondrial DNMT1 During Oxidative Stress. [Internet] [Thesis]. Virginia Commonwealth University; 2012. [cited 2021 Mar 02]. Available from: https://doi.org/10.25772/4ZS0-8P80 ; https://scholarscompass.vcu.edu/etd/2826.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Balinang J. The Regulation of Mitochondrial DNMT1 During Oxidative Stress. [Thesis]. Virginia Commonwealth University; 2012. Available from: https://doi.org/10.25772/4ZS0-8P80 ; https://scholarscompass.vcu.edu/etd/2826

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Virginia Commonwealth University

23. Endisha, Helal. SIRT6 and Premature Aging of Hutchinson-Gilford Progeria Syndrome Fibroblasts.

Degree: MS, Microbiology & Immunology, 2013, Virginia Commonwealth University

URL: https://doi.org/10.25772/7RTM-VT52 ; https://scholarscompass.vcu.edu/etd/3238

► The genetic disease Hutchinson-Gilford Progeria Syndrome (HGPS) arises from a de novo single nucleotide mutation (1824CàT) in the LMNA gene. As a result, the mutated… (more)

Subjects/Keywords: Medicine and Health Sciences

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APA (6^th Edition):

Endisha, H. (2013). SIRT6 and Premature Aging of Hutchinson-Gilford Progeria Syndrome Fibroblasts. (Thesis). Virginia Commonwealth University. Retrieved from https://doi.org/10.25772/7RTM-VT52 ; https://scholarscompass.vcu.edu/etd/3238

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Endisha, Helal. “SIRT6 and Premature Aging of Hutchinson-Gilford Progeria Syndrome Fibroblasts.” 2013. Thesis, Virginia Commonwealth University. Accessed March 02, 2021. https://doi.org/10.25772/7RTM-VT52 ; https://scholarscompass.vcu.edu/etd/3238.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Endisha, Helal. “SIRT6 and Premature Aging of Hutchinson-Gilford Progeria Syndrome Fibroblasts.” 2013. Web. 02 Mar 2021.

Vancouver:

Endisha H. SIRT6 and Premature Aging of Hutchinson-Gilford Progeria Syndrome Fibroblasts. [Internet] [Thesis]. Virginia Commonwealth University; 2013. [cited 2021 Mar 02]. Available from: https://doi.org/10.25772/7RTM-VT52 ; https://scholarscompass.vcu.edu/etd/3238.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Endisha H. SIRT6 and Premature Aging of Hutchinson-Gilford Progeria Syndrome Fibroblasts. [Thesis]. Virginia Commonwealth University; 2013. Available from: https://doi.org/10.25772/7RTM-VT52 ; https://scholarscompass.vcu.edu/etd/3238

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Virginia Commonwealth University

24. MISTRY, SHRENI. Characterization of potential interactions between transferrin binding proteins in Neisseria gonorrhoeae.

Degree: PhD, Microbiology & Immunology, 2013, Virginia Commonwealth University

URL: https://doi.org/10.25772/8ZT6-1A88 ; https://scholarscompass.vcu.edu/etd/2954

► Neisseria gonorrhoeae requires iron for survival and establishment of infection in the human host. Pathogenic Neisseriae have evolved a repertoire of high-affinity iron acquisition systems… (more)

▼ Neisseria gonorrhoeae requires iron for survival and establishment of infection in the human host. Pathogenic Neisseriae have evolved a repertoire of high-affinity iron acquisition systems to facilitate iron uptake in the human host. This requires specific outer membrane receptors and energy-harnessing cytoplasmic membrane proteins. The transferrin receptor proteins of Neisseria gonorrhoeae are necessary for iron uptake from transferrin in the host. The iron uptake system consists of two transferrin binding proteins, (Tbp) A and B. TbpA is an integral outer membrane, TonB-dependent transporter that forms the pore for iron internalization. TbpB is a surface exposed lipoprotein that makes the iron internalization process more efficient. TbpA is proposed to consist of two distinct domains: a β barrel and an N-terminal plug domain. Previous studies have shown that the EIEYE sequence in the TbpA plug domain plays an important role in iron internalization. We undertook a collaborative project to test the hypothesis that the conserved EIEYE sequence in the wild-type TbpA plug binds Fe3+ during the outer membrane iron transport process. CD spectra analysis and fluorescence emission titration spectra of purified recombinant wildtype and mutated plug proteins revealed that Fe3+ is sequestered by the wildtype TbpA plug protein, unlike the mutated plug protein. Modeling data with the wild-type plug predicts the EIEYE sequence is part of a flexible loop structure and acts as an Fe3+ binding site. Characterization of the Tbps constituting the gonococcal receptor is important to understanding how the gonococcus survives within its host. TbpA and TbpB act together to acquire iron from human transferrin. We hypothesize that the presence of TbpA impacts the exposure or conformation of TbpB. In this study, we have utilized photoactivable cross-linkers to assess the effect of TbpA on TbpB in live gonococcal cells and studied it in presence of ligand and TonB derived energy. We employed insertion mutants, in which TbpA and TbpB contained the hemagglutinin (HA) epitope tag, to probe for impact of TbpA on TbpB. Our results demonstrate that photo-cross-linking altered TbpB size and migration and was dependent on the presence of TbpA. HA epitope insertion mutants in surface exposed loops of TbpA and TbpB did not impact the mobility of cross-linked TbpB. Addition of human transferrin to the de-energized mutant caused a change in TbpB migration after cross-linking. This result indicates that when ligand is bound tightly and irreversibly to de-energized TbpA, the surface accessibility and perhaps conformation of TbpB is altered and TbpA does not interact with TbpB. Our findings were confirmed with recent structural studies of TbpA-TbpB-ligand triple complex, which illustrate that Tbps bind ligand through unique, non-overlapping binding sites such that TbpA and TbpB do not interact. TbpB is not an essential member of transferrin-iron acquisition pathway. It is surface exposed and tethered to the outer membrane by a lipid moiety. The role of… Advisors/Committee Members: CYNTHIA CORNELISSEN.

Subjects/Keywords: Medicine and Health Sciences

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

MISTRY, S. (2013). Characterization of potential interactions between transferrin binding proteins in Neisseria gonorrhoeae. (Doctoral Dissertation). Virginia Commonwealth University. Retrieved from https://doi.org/10.25772/8ZT6-1A88 ; https://scholarscompass.vcu.edu/etd/2954

Chicago Manual of Style (16^th Edition):

MISTRY, SHRENI. “Characterization of potential interactions between transferrin binding proteins in Neisseria gonorrhoeae.” 2013. Doctoral Dissertation, Virginia Commonwealth University. Accessed March 02, 2021. https://doi.org/10.25772/8ZT6-1A88 ; https://scholarscompass.vcu.edu/etd/2954.

MLA Handbook (7^th Edition):

MISTRY, SHRENI. “Characterization of potential interactions between transferrin binding proteins in Neisseria gonorrhoeae.” 2013. Web. 02 Mar 2021.

Vancouver:

MISTRY S. Characterization of potential interactions between transferrin binding proteins in Neisseria gonorrhoeae. [Internet] [Doctoral dissertation]. Virginia Commonwealth University; 2013. [cited 2021 Mar 02]. Available from: https://doi.org/10.25772/8ZT6-1A88 ; https://scholarscompass.vcu.edu/etd/2954.

Council of Science Editors:

MISTRY S. Characterization of potential interactions between transferrin binding proteins in Neisseria gonorrhoeae. [Doctoral Dissertation]. Virginia Commonwealth University; 2013. Available from: https://doi.org/10.25772/8ZT6-1A88 ; https://scholarscompass.vcu.edu/etd/2954

Virginia Commonwealth University

25. Gaitonde, Supriya A. A study of the action of risperidone at 5-HT2A receptors.

Degree: PhD, Pharmaceutical Sciences, 2016, Virginia Commonwealth University

URL: https://doi.org/10.25772/8G6W-0G60 ; https://scholarscompass.vcu.edu/etd/4112

► Risperidone is an ‘atypical’ antipsychotic and is approved by the USFDA mainly for the treatment of schizophrenia and symptoms of bipolar disorder. Risperidone (an… (more)

▼ Risperidone is an ‘atypical’ antipsychotic and is approved by the USFDA mainly for the treatment of schizophrenia and symptoms of bipolar disorder. Risperidone (an SDA or serotonin-dopamine antagonist) has ~20-fold higher affinity at 5-HT_2A receptors over dopamine D₂ receptors, which makes it more efficacious against the negative symptoms of schizophrenia and less liable to causing extrapyramidal side effects than ‘typical’ antipsychotics. The major goal of the current investigation was to study the structure of risperidone and to identify the minimum structural features required for 5-HT_2A receptor affinity that retain antagonist action. The structure of risperidone was systematically deconstructed, and functional activity studies using calcium imaging in HEK293 cells and a two-electrode voltage clamp (TEVC) assay in a Xenopus laevis heterologous system were coupled with radioligand binding affinity studies to achieve this goal. The biological studies showed that the entire structure of risperidone was not required for activity or affinity at the receptor, as 6-fluoro-[3-(1-methylpiperidin-4-yl)]benz[d]isoxazole was comparable to risperidone in both affinity and activity. Next, the structure of risperidone was elaborated to determine the importance of its left and right “halves” in its actions. The left and the right halves of risperidone were substituted with those of another antagonist, ketanserin, to give structural hybrids. Biological studies suggested that the right half of risperidone [i.e., the 6-fluoro-(3-piperidin-4-yl)benz[d]isoxazole moiety] might be important for affinity. In order to assess how the biologically-active compounds interact at the receptor, homology models of the human 5-HT_2A receptor were developed, and docking and Hydropathic INTeraction studies were conducted. Risperidone seemed to form a bifurcated hydrogen bond with S159 (TM3), which ketanserin was unable to form. This interaction might account for high binding affinity at the receptor as it is common to both, risperidone and 3-[2-(4-(6-fluorobenz[d]isoxazol-3-yl)piperidin-1-yl)ethyl]-2,4-(1H,3H)quinazolinedione. With the data currently in hand, we can conclude that the entire structure of risperidone is not required for activity or affinity, and that the right “half” (i.e. the benzisoxazolyl portion) of risperidone might be influencing activity and affinity at 5-HT_2A receptors. Advisors/Committee Members: Dr. Malgorzata Dukat, Dr. Richard A. Glennon.

Subjects/Keywords: Medicine and Health Sciences

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APA (6^th Edition):

Gaitonde, S. A. (2016). A study of the action of risperidone at 5-HT2A receptors. (Doctoral Dissertation). Virginia Commonwealth University. Retrieved from https://doi.org/10.25772/8G6W-0G60 ; https://scholarscompass.vcu.edu/etd/4112

Chicago Manual of Style (16^th Edition):

Gaitonde, Supriya A. “A study of the action of risperidone at 5-HT2A receptors.” 2016. Doctoral Dissertation, Virginia Commonwealth University. Accessed March 02, 2021. https://doi.org/10.25772/8G6W-0G60 ; https://scholarscompass.vcu.edu/etd/4112.

MLA Handbook (7^th Edition):

Gaitonde, Supriya A. “A study of the action of risperidone at 5-HT2A receptors.” 2016. Web. 02 Mar 2021.

Vancouver:

Gaitonde SA. A study of the action of risperidone at 5-HT2A receptors. [Internet] [Doctoral dissertation]. Virginia Commonwealth University; 2016. [cited 2021 Mar 02]. Available from: https://doi.org/10.25772/8G6W-0G60 ; https://scholarscompass.vcu.edu/etd/4112.

Council of Science Editors:

Gaitonde SA. A study of the action of risperidone at 5-HT2A receptors. [Doctoral Dissertation]. Virginia Commonwealth University; 2016. Available from: https://doi.org/10.25772/8G6W-0G60 ; https://scholarscompass.vcu.edu/etd/4112

University of Wisconsin – Milwaukee

26. Chawla, Ayesha. Regulation of Anti-Angiogenic Pedf Through a TSP-1 - CD36 Pathway in Prostate Cancer.

Degree: MS, Biomedical Sciences, 2013, University of Wisconsin – Milwaukee

URL: https://dc.uwm.edu/etd/672

► Prostate cancer (PCa) is the most common type of cancer diagnosed in American men. Cancer progression is associated with increased angiogenesis, and thrombospondin-1 (TSP–1)… (more)

▼ Prostate cancer (PCa) is the most common type of cancer diagnosed in American men. Cancer progression is associated with increased angiogenesis, and thrombospondin-1 (TSP–1) and pigment epithelium derived factor (PEDF), both potent anti-angiogenic molecules, are downregulated in PCa cells. TSP-1 exerts its activity through binding to several cell surface receptors such as CD36. Both TSP-1 and PEDF are multi-functional proteins and have been linked to lipid metabolism in other cell types. Moreover, TSP-1 - PEDF regulatory loops have been identified in some cell types. PEDF has been shown to inhibit PCa growth through its effects on angiogenesis and directly on the PCa cells; however, how PEDF expression levels are regulated in prostate cells is currently unknown. Here, we hypothesized that TSP-1 may regulate PEDF expression and lipid metabolism in PCa cells. I collected and examined PEDF levels in both cell lysate and serum-free conditioned media samples from TSP-1 and anti-CD36 antibody treated prostate cells and examined PEDF levels. Both TSP-1 and anti-CD36 treatment increased PEDF expression in normal prostate epithelial cells, RWPE-1, and in PCa cells, PC-3 and LNCaP. The expression of candidate TSP-1 - CD36 signaling mediators, fyn, p38 MAPK and JNK, were also examined in treated samples. I found that TSP-1 treatment elevated expression of fyn, p38 and JNK in PC-3 and DU145 cells. In contrast, blocking the CD36 receptor diminished the expression of each signaling mediator. My results are the first to show that a regulatory loop exists between TSP-1 and PEDF in prostate cells. The observation that treatment with anti-CD36 antibody also increased PEDF suggests that TSP-1 regulation of PEDF may be mediated through the CD36 receptor. These observations suggest that one mechanism of PEDF down-regulation in PCa cells may be due to loss of TSP-1 expression. Moreover, this pathway could be exploited for novel therapeutic interventions. Advisors/Committee Members: Jennifer A. Doll.

Subjects/Keywords: Medicine and Health Sciences

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Chawla, A. (2013). Regulation of Anti-Angiogenic Pedf Through a TSP-1 - CD36 Pathway in Prostate Cancer. (Thesis). University of Wisconsin – Milwaukee. Retrieved from https://dc.uwm.edu/etd/672

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Chawla, Ayesha. “Regulation of Anti-Angiogenic Pedf Through a TSP-1 - CD36 Pathway in Prostate Cancer.” 2013. Thesis, University of Wisconsin – Milwaukee. Accessed March 02, 2021. https://dc.uwm.edu/etd/672.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Chawla, Ayesha. “Regulation of Anti-Angiogenic Pedf Through a TSP-1 - CD36 Pathway in Prostate Cancer.” 2013. Web. 02 Mar 2021.

Vancouver:

Chawla A. Regulation of Anti-Angiogenic Pedf Through a TSP-1 - CD36 Pathway in Prostate Cancer. [Internet] [Thesis]. University of Wisconsin – Milwaukee; 2013. [cited 2021 Mar 02]. Available from: https://dc.uwm.edu/etd/672.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Chawla A. Regulation of Anti-Angiogenic Pedf Through a TSP-1 - CD36 Pathway in Prostate Cancer. [Thesis]. University of Wisconsin – Milwaukee; 2013. Available from: https://dc.uwm.edu/etd/672

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Wisconsin – Milwaukee

27. Sheerin, Shabistan. Innovation and Commercialization Practices - A Qualitative Analysis of Novascan LLC.

Degree: PhD, Health Sciences, 2013, University of Wisconsin – Milwaukee

URL: https://dc.uwm.edu/etd/760

► The processes for commercialization of medical devices in healthcare are complex and varied, and it has been difficult to define the ingredients of success.… (more)

▼ The processes for commercialization of medical devices in healthcare are complex and varied, and it has been difficult to define the ingredients of success. There exists a need to better understand evidence based best practices as there is lack of documented evidence based on best practices for commercialization of medical devices by startups. Commercialization of innovative medical devices in healthcare is in constant demand and the reasons are many fold. Most of the research based startups act as agents of economic development and therefore they need to function more efficiently and effectively. There exists a constant demand from end users to improve medical techniques, results patient experiences and cost effectiveness. However, a large number of strong and commercially viable innovations in healthcare fail to achieve commercialization. The purpose of this paper is to build a theory. The study examines qualitatively commercialization practices of case study NovaScan LLC, a breast cancer detection device company. Through this single case study, various performances indicators of the commercialization steps followed by the company are identified and findings are presented in the form of theoretical propositions. Extensive literature review and analysis helped in better understanding of the process of commercialization from both healthcare and non-healthcare perspective. Data gathering, which focused on the above mentioned aim was carried on for nearly over four years, initially as an outsider participant and then in the latter part of study, as an insider participant. The data consisted of observations, informal conversations both via telephone and in-person, using an unstructured interview protocol, field notes, company archives and other historical data. Data collection participants were those institutional officials who were responsible both directly and indirectly for the innovation and commercialization activities at NovaScan LLC. All observations, conversations, field notes, documents and other records have been documented. The analysis for this study involves continuous back and forth linking of theory presented by literature findings and data obtained at NovaScan LLC. For the purpose of data analysis, the data is not coded sentence by sentence; rather it's focused on theme identification based on underlying meaning. The results verify the impressions of many practitioners in the field of innovation and commercialization of medical devices in healthcare. The findings are presented in the form of seven propositions and also propose a framework of commercialization. These findings are recommended to be tested in future. Various activities related to commercialization process do not happen in isolation with product development in a startup firm like NovaScan LLC. Commercialization strategies are an integral part of development work and are well-aligned with the development process and all stages of development process overlap with each other. Advisors/Committee Members: Timothy B. Patrick.

Subjects/Keywords: Business; Medicine and Health Sciences

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Sheerin, S. (2013). Innovation and Commercialization Practices - A Qualitative Analysis of Novascan LLC. (Doctoral Dissertation). University of Wisconsin – Milwaukee. Retrieved from https://dc.uwm.edu/etd/760

Chicago Manual of Style (16^th Edition):

Sheerin, Shabistan. “Innovation and Commercialization Practices - A Qualitative Analysis of Novascan LLC.” 2013. Doctoral Dissertation, University of Wisconsin – Milwaukee. Accessed March 02, 2021. https://dc.uwm.edu/etd/760.

MLA Handbook (7^th Edition):

Sheerin, Shabistan. “Innovation and Commercialization Practices - A Qualitative Analysis of Novascan LLC.” 2013. Web. 02 Mar 2021.

Vancouver:

Sheerin S. Innovation and Commercialization Practices - A Qualitative Analysis of Novascan LLC. [Internet] [Doctoral dissertation]. University of Wisconsin – Milwaukee; 2013. [cited 2021 Mar 02]. Available from: https://dc.uwm.edu/etd/760.

Council of Science Editors:

Sheerin S. Innovation and Commercialization Practices - A Qualitative Analysis of Novascan LLC. [Doctoral Dissertation]. University of Wisconsin – Milwaukee; 2013. Available from: https://dc.uwm.edu/etd/760

Texas Medical Center

28. Khalili, Jahan. ROLES FOR BRAF KINASE ACTIVATING MUTATIONS IN MELANOMA: MICROENVIRONMENTAL IMMUNOSUPPRESSION.

Degree: PhD, 2011, Texas Medical Center

URL: https://digitalcommons.library.tmc.edu/utgsbs_dissertations/213

► The BRAF oncogene demonstrates a characteristic mutation (V600E) in a significant fraction of cutaneous melanomas, leading to constitutive activation of the MAP kinase pathway.… (more)

Subjects/Keywords: Melanoma; Medicine and Health Sciences

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Khalili, J. (2011). ROLES FOR BRAF KINASE ACTIVATING MUTATIONS IN MELANOMA: MICROENVIRONMENTAL IMMUNOSUPPRESSION. (Doctoral Dissertation). Texas Medical Center. Retrieved from https://digitalcommons.library.tmc.edu/utgsbs_dissertations/213

Chicago Manual of Style (16^th Edition):

Khalili, Jahan. “ROLES FOR BRAF KINASE ACTIVATING MUTATIONS IN MELANOMA: MICROENVIRONMENTAL IMMUNOSUPPRESSION.” 2011. Doctoral Dissertation, Texas Medical Center. Accessed March 02, 2021. https://digitalcommons.library.tmc.edu/utgsbs_dissertations/213.

MLA Handbook (7^th Edition):

Khalili, Jahan. “ROLES FOR BRAF KINASE ACTIVATING MUTATIONS IN MELANOMA: MICROENVIRONMENTAL IMMUNOSUPPRESSION.” 2011. Web. 02 Mar 2021.

Vancouver:

Khalili J. ROLES FOR BRAF KINASE ACTIVATING MUTATIONS IN MELANOMA: MICROENVIRONMENTAL IMMUNOSUPPRESSION. [Internet] [Doctoral dissertation]. Texas Medical Center; 2011. [cited 2021 Mar 02]. Available from: https://digitalcommons.library.tmc.edu/utgsbs_dissertations/213.

Council of Science Editors:

Khalili J. ROLES FOR BRAF KINASE ACTIVATING MUTATIONS IN MELANOMA: MICROENVIRONMENTAL IMMUNOSUPPRESSION. [Doctoral Dissertation]. Texas Medical Center; 2011. Available from: https://digitalcommons.library.tmc.edu/utgsbs_dissertations/213

Texas Medical Center

29. Rodriguez-Cruz, Tania G. THE CYTOPLASMIC TAIL OF MHC CLASS I MOLECULES PLAYS A CRITICAL ROLE IN DENDRITIC CELL-INDUCED T CELL IMMUNITY.

Degree: PhD, 2011, Texas Medical Center

URL: https://digitalcommons.library.tmc.edu/utgsbs_dissertations/243

► The presentation of MHC class I (MHC-I)/peptide complexes by dendritic cells (DCs) is critical for the maintenance of central tolerance to self and for… (more)

▼ The presentation of MHC class I (MHC-I)/peptide complexes by dendritic cells (DCs) is critical for the maintenance of central tolerance to self and for the regulation of cytotoxic T lymphocytes (CTL)-mediated adaptive immune responses against pathogens and cancer cells. Interestingly, several findings have suggested that the cytoplasmic tail of MHC class I plays a functional role in the regulation of CTL immune responses. For example, our previous studies demonstrated that exon 7-deleted MHC-I molecules not only showed extended DC cell surface half-lives but also induced significantly increased CTL responses to viral challange invivo. Although exon 7-deleted variant of MHC-I does not occur naturally in humans, the animal studies prompted us to examine whether exon 7-deleted MHC-I molecules could generate augmented CTL responses in a therapeutic DC-based vaccine setting. To examine the stimulatory capacity of exon 7-deleted MHC-I molecules, we generated a lentivirus-mediated gene transfer system to induce the expression of different MHC-I cytoplasmic tail isoforms in both mouse and human DCs. These DCs were then used as vaccines in a melanoma mouse tumor model and in a human invitro co-culture system. In this thesis, we show that DCs expressing exon 7-deleted MHC-I molecules, stimulated remarkably higher levels of T-cell cytokine production and significantly increased the proliferation of meanoma-specific (Pmel-1) T cells compared with DCs expressing wild type MHC-I. We also demonstrate that, in combination with adoptive transfer of Pmel-1 T-cell, DCs expressing exon 7-deleted Db molecules induced greater anti-tumor responses against established B16 melanoma tumors, significantly extending mouse survival as compared to DCs expressing wild-type Db molecules. Moreover, we also observed that human DCs expressing exon 7-deleted HLA-A2 molecules showed similarly augmented CTL stimulatory ability. Mechanistic studies suggest that exon 7-deleted MHC-I molecules showed impaired lateral membrane movement and extended cell surface half-lives within the DC/T-cell interface, leading to increased spatial availability of MHC-I/peptide complexes for recognition by CD8+ T cells. Collectively, these results suggesr that targeting exon 7 within the cytoplasmic tail of MHC-I molecules in DC vaccines has the potential to enhance CD8+ T cell stimulatory capacity and improve clinical outcomes in patients with cancer or viral infections. Advisors/Committee Members: Gregory Lizee, Patrick Hwu, Stephanie Watowich.

Subjects/Keywords: Immunity; Medicine and Health Sciences

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Rodriguez-Cruz, T. G. (2011). THE CYTOPLASMIC TAIL OF MHC CLASS I MOLECULES PLAYS A CRITICAL ROLE IN DENDRITIC CELL-INDUCED T CELL IMMUNITY. (Doctoral Dissertation). Texas Medical Center. Retrieved from https://digitalcommons.library.tmc.edu/utgsbs_dissertations/243

Chicago Manual of Style (16^th Edition):

Rodriguez-Cruz, Tania G. “THE CYTOPLASMIC TAIL OF MHC CLASS I MOLECULES PLAYS A CRITICAL ROLE IN DENDRITIC CELL-INDUCED T CELL IMMUNITY.” 2011. Doctoral Dissertation, Texas Medical Center. Accessed March 02, 2021. https://digitalcommons.library.tmc.edu/utgsbs_dissertations/243.

MLA Handbook (7^th Edition):

Rodriguez-Cruz, Tania G. “THE CYTOPLASMIC TAIL OF MHC CLASS I MOLECULES PLAYS A CRITICAL ROLE IN DENDRITIC CELL-INDUCED T CELL IMMUNITY.” 2011. Web. 02 Mar 2021.

Vancouver:

Rodriguez-Cruz TG. THE CYTOPLASMIC TAIL OF MHC CLASS I MOLECULES PLAYS A CRITICAL ROLE IN DENDRITIC CELL-INDUCED T CELL IMMUNITY. [Internet] [Doctoral dissertation]. Texas Medical Center; 2011. [cited 2021 Mar 02]. Available from: https://digitalcommons.library.tmc.edu/utgsbs_dissertations/243.

Council of Science Editors:

Rodriguez-Cruz TG. THE CYTOPLASMIC TAIL OF MHC CLASS I MOLECULES PLAYS A CRITICAL ROLE IN DENDRITIC CELL-INDUCED T CELL IMMUNITY. [Doctoral Dissertation]. Texas Medical Center; 2011. Available from: https://digitalcommons.library.tmc.edu/utgsbs_dissertations/243

Texas Medical Center

30. Charles, Catherine T. PHENOTHIAZINES INDUCE ABORTIVE AUTOPHAGY LEADING TO CANCER CELL DEATH.

Degree: PhD, 2012, Texas Medical Center

URL: https://digitalcommons.library.tmc.edu/utgsbs_dissertations/294

► Autophagy is an evolutionarily conserved process that functions to maintain homeostasis and provides energy during nutrient deprivation and environmental stresses for the survival of… (more)

▼ Autophagy is an evolutionarily conserved process that functions to maintain homeostasis and provides energy during nutrient deprivation and environmental stresses for the survival of cells by delivering cytoplasmic contents to the lysosomes for recycling and energy generation. Dysregulation of this process has been linked to human diseases including immune disorders, neurodegenerative muscular diseases and cancer. Autophagy is a double edged sword in that it has both pro-survival and pro-death roles in cancer cells. Its cancer suppressive roles include the clearance of damaged organelles, which could otherwise lead to inflammation and therefore promote tumorigenesis. In its pro-survival role, autophagy allows cancer cells to overcome cytotoxic stresses generated the cancer environment or cancer treatments such as chemotherapy and evade cell death. A better understanding of how drugs that perturb autophagy affect cancer cell signaling is of critical importance toimprove the cancer treatment arsenal. In order to gain insights in the relationship between autophagy and drug treatments, we conducted a high-throughput drug screen to identify autophagy modulators. Our high-throughput screen utilized image based fluorescent microscopy for single cell analysis to identify chemical perturbants of the autophagic process. Phenothiazines emerged as the largest family of drugs that alter the autophagic process by increasing LC3-II punctae levels in different cancer cell lines. In addition, we observed multiple biological effects in cancer cells treated with phenothiazines. Those antitumorigenic effects include decreased cell migration, cell viability, and ATP production along with abortive autophagy. Our studies highlight the potential role of phenothiazines as agents for combinational therapy with other chemotherapeutic agents in the treatment of different cancers. Advisors/Committee Members: Gordon B. Mills, M.D., Ph.D., Peter J. Davies, M.D., Ph.D., Honami Naora, Ph.D..

Subjects/Keywords: Medicine and Health Sciences

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APA (6^th Edition):

Charles, C. T. (2012). PHENOTHIAZINES INDUCE ABORTIVE AUTOPHAGY LEADING TO CANCER CELL DEATH. (Doctoral Dissertation). Texas Medical Center. Retrieved from https://digitalcommons.library.tmc.edu/utgsbs_dissertations/294

Chicago Manual of Style (16^th Edition):

Charles, Catherine T. “PHENOTHIAZINES INDUCE ABORTIVE AUTOPHAGY LEADING TO CANCER CELL DEATH.” 2012. Doctoral Dissertation, Texas Medical Center. Accessed March 02, 2021. https://digitalcommons.library.tmc.edu/utgsbs_dissertations/294.

MLA Handbook (7^th Edition):

Charles, Catherine T. “PHENOTHIAZINES INDUCE ABORTIVE AUTOPHAGY LEADING TO CANCER CELL DEATH.” 2012. Web. 02 Mar 2021.

Vancouver:

Charles CT. PHENOTHIAZINES INDUCE ABORTIVE AUTOPHAGY LEADING TO CANCER CELL DEATH. [Internet] [Doctoral dissertation]. Texas Medical Center; 2012. [cited 2021 Mar 02]. Available from: https://digitalcommons.library.tmc.edu/utgsbs_dissertations/294.

Council of Science Editors:

Charles CT. PHENOTHIAZINES INDUCE ABORTIVE AUTOPHAGY LEADING TO CANCER CELL DEATH. [Doctoral Dissertation]. Texas Medical Center; 2012. Available from: https://digitalcommons.library.tmc.edu/utgsbs_dissertations/294

Open Access Theses and Dissertations