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1.
Pangrazzi, Elizabeth.
The Rural Provider's Perspective| Conversations With Patients About Mental Health.
Degree: 2017, Union Institute and University
URL: http://pqdtopen.proquest.com/#viewpdf?dispub=10624346 
► The rural primary care provider is likely to treat patients with mental health issues in rural populations due to lack of mental health providers,…
(more)
▼ The rural primary care provider is likely to treat patients with mental health issues in rural populations due to lack of mental health providers, comorbidity and multimorbidity of patient illness, and stigma associated with seeking mental health services. The very nature of rural primary care allows for patients to be comfortable with their PCP and therefore, rely on the strong foundational relationship they have in entrusting them with their mental health concerns. Being a primary care provider in a rural setting offers both rewards and challenges unique to the rural culture. The main goal of the study was to explore what might enhance, diminish, or otherwise give meaning to the rural primary care provider’s experience of engaging in discussions about mental health issues with their patients. This was achieved through identifying themes in response to two primary research questions. The primary research questions were: (a) How do rural primary care providers engage in conversations about mental health issues with their patients?; and (b) What are factors of being in a rural setting that affect and/or influence the primary care provider in having these conversations? Data from ten participants across the three rural primary care clinics was analyzed using the qualitative method of thematic analysis to identify embedded themes. Five themes were revealed: (a) The relationship between the patient and the rural primary care provider allows for the conversation about mental health issues; (b) Rural primary care providers routinely provide mental health services; (c) Rural primary care providers experience challenges treating complex mental health issues; (d) Rural primary care providers navigate barriers in rural communities; and (e) There are benefits and drawbacks of rural living.
Subjects/Keywords: Mental health; Medicine; Health sciences
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APA (6th Edition):
Pangrazzi, E. (2017). The Rural Provider's Perspective| Conversations With Patients About Mental Health. (Thesis). Union Institute and University. Retrieved from http://pqdtopen.proquest.com/#viewpdf?dispub=10624346
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Pangrazzi, Elizabeth. “The Rural Provider's Perspective| Conversations With Patients About Mental Health.” 2017. Thesis, Union Institute and University. Accessed June 23, 2018.
http://pqdtopen.proquest.com/#viewpdf?dispub=10624346.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Pangrazzi, Elizabeth. “The Rural Provider's Perspective| Conversations With Patients About Mental Health.” 2017. Web. 23 Jun 2018.
Vancouver:
Pangrazzi E. The Rural Provider's Perspective| Conversations With Patients About Mental Health. [Internet] [Thesis]. Union Institute and University; 2017. [cited 2018 Jun 23].
Available from: http://pqdtopen.proquest.com/#viewpdf?dispub=10624346.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Pangrazzi E. The Rural Provider's Perspective| Conversations With Patients About Mental Health. [Thesis]. Union Institute and University; 2017. Available from: http://pqdtopen.proquest.com/#viewpdf?dispub=10624346
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
McMaster University
2.
MacLeod, Natalie T.
A controlled cluster randomized pilot study of the effect of a new smoking cessation management module on rates of initiation and continuation of smoking counselling in Ontario primary care practices using P-PROMPT Chronic Disease Management System (CDMS).
Degree: MS, 2010, McMaster University
URL: http://hdl.handle.net/11375/12299
► Multi-faceted interventions that include some form of a clinical information system have been shown to improve primary care physicians' management of chronic diseases. The…
(more)
▼ Multi-faceted interventions that include some form of a clinical information system have been shown to improve primary care physicians' management of chronic diseases. The objective of this pilot study was to assess the feasibility of a cluster randomized controlled trial of a multi-faceted intervention, which includes a clinical information system, to improve the management of the chronic disease of tobacco use by physicians. Feasibility was assessed with respect to the use of a measurement tool (Smoking Status Identification Card) and use of a new smoking cessation management module in the clinical information system. Letters of invitation were sent out to the 65 primary care physicians (in 38 primary care practices) who were subscribed to the web-based clinical information system (P-PROMPT CDMS). Five physicians from 5 primary care practices agree.d to participate, who were stratified and then randomized to the intervention (2 primary care practices) or control group (3 primary care practices). Following the 12-week study period, SSIC completion reached the 90% threshold success criterion in 2 of the 5 primary care practices (one each from the intervention and control group). The intervention group demonstrated basic use of the new smoking cessation management module that reached 21.9% and 19.0% in each of the respective practices, which was below the 30% threshold success criterion. A preliminary evaluation of physician delivery of smoking cessation counselling demonstrated a trend to a higher percentage of Ministry of Health and Long-Term Care (MOHLTC) physician service billing codes submitted among the physicians in the intervention group, which may be indicative of greater smoking cessation counselling. It is concluded that a randomized controlled trial to test a multi-faceted intervention is not feasible with the current study design. Significant modifications to the current study design are required that can potentially be tested prior to progression to a larger trial.
Master of Science (MS)
Advisors/Committee Members: Sebaldt, Rolf J., Health Research Methodology.
Subjects/Keywords: Health Research Methodology; Medicine and Health Sciences; Medicine and Health Sciences
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APA (6th Edition):
MacLeod, N. T. (2010). A controlled cluster randomized pilot study of the effect of a new smoking cessation management module on rates of initiation and continuation of smoking counselling in Ontario primary care practices using P-PROMPT Chronic Disease Management System (CDMS). (Masters Thesis). McMaster University. Retrieved from http://hdl.handle.net/11375/12299
Chicago Manual of Style (16th Edition):
MacLeod, Natalie T. “A controlled cluster randomized pilot study of the effect of a new smoking cessation management module on rates of initiation and continuation of smoking counselling in Ontario primary care practices using P-PROMPT Chronic Disease Management System (CDMS).” 2010. Masters Thesis, McMaster University. Accessed June 23, 2018.
http://hdl.handle.net/11375/12299.
MLA Handbook (7th Edition):
MacLeod, Natalie T. “A controlled cluster randomized pilot study of the effect of a new smoking cessation management module on rates of initiation and continuation of smoking counselling in Ontario primary care practices using P-PROMPT Chronic Disease Management System (CDMS).” 2010. Web. 23 Jun 2018.
Vancouver:
MacLeod NT. A controlled cluster randomized pilot study of the effect of a new smoking cessation management module on rates of initiation and continuation of smoking counselling in Ontario primary care practices using P-PROMPT Chronic Disease Management System (CDMS). [Internet] [Masters thesis]. McMaster University; 2010. [cited 2018 Jun 23].
Available from: http://hdl.handle.net/11375/12299.
Council of Science Editors:
MacLeod NT. A controlled cluster randomized pilot study of the effect of a new smoking cessation management module on rates of initiation and continuation of smoking counselling in Ontario primary care practices using P-PROMPT Chronic Disease Management System (CDMS). [Masters Thesis]. McMaster University; 2010. Available from: http://hdl.handle.net/11375/12299
3.
Gottlieb, Eric Raphael.
Humoral Immune Responses to a Malaria Vaccine Candidate| Towards a Correlate of Vaccine-Induced Protection.
Degree: 2012, University of Maryland, Baltimore
URL: http://pqdtopen.proquest.com/#viewpdf?dispub=1512511
► <i>Introduction:</i> Identifying immune correlates of protection is a priority for malaria vaccine research. A successful pediatric Phase 2 clinical trial in Mali of FMP2.1/AS02A,…
(more)
▼ <i>Introduction:</i> Identifying immune correlates of protection is a priority for malaria vaccine research. A successful pediatric Phase 2 clinical trial in Mali of FMP2.1/AS02A, a recombinant apical membrane antigen 1 (AMA1)-based vaccine candidate, provided a source of serum samples from subjects who may have developed vaccine-induced, strain-specific protective immunity to clinical malaria illness. We studied IgG subclass and avidity patterns of antibodies to the malaria protein AMA1 in a subset of participants, with the objective of identifying immune responses that may be associated with protection against malaria. We hypothesized that the AMA1 vaccine candidate would induce production of cytophilic antibody subclasses IgG1 and IgG3, as well as overall IgG avidity maturation. <i>Methods:</i> Titers of IgG1, IgG2, IgG3, and IgG4, as well as avidity of antibodies to AMA1, were determined by ELISA for ten AMA1 vaccine recipients and ten control subjects who had been randomized to receive a rabies vaccine in this double-blind trial at days 0, 90, and 150 after the first of three vaccinations. To identify statistically significant differences between the groups, responses in vaccine recipients were evaluated longitudinally and compared with responses in control subjects. <i>Results:</i> IgG1, IgG2, IgG3, and IgG4 were induced more strongly in vaccine recipients than in control subjects. Additionally, vaccine recipients had higher ratios of cytophilic to non-cytophilic antibodies than control subjects. Avidity indices were not significantly different between the two groups at the three time points tested, and there were no significant differences in avidity between time points in either group. <i>Conclusion:</i> Contrary to our hypothesis, both cytophilic and non-cytophilic antibodies were induced by the FMP2.1/AS02A vaccine candidate and immunization did not appear to stimulate avidity maturation. Therefore, IgG1, IgG2, IgG3, and IgG4 titers are candidate variables for a humoral immune correlate of vaccine-induced protection. These results are among the first reports of the subclasses and avidity of antibodies produced in response to a malaria vaccine candidate with allele-specific protective efficacy.
Subjects/Keywords: Health Sciences; Medicine and Surgery
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APA (6th Edition):
Gottlieb, E. R. (2012). Humoral Immune Responses to a Malaria Vaccine Candidate| Towards a Correlate of Vaccine-Induced Protection. (Thesis). University of Maryland, Baltimore. Retrieved from http://pqdtopen.proquest.com/#viewpdf?dispub=1512511
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Gottlieb, Eric Raphael. “Humoral Immune Responses to a Malaria Vaccine Candidate| Towards a Correlate of Vaccine-Induced Protection.” 2012. Thesis, University of Maryland, Baltimore. Accessed June 23, 2018.
http://pqdtopen.proquest.com/#viewpdf?dispub=1512511.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Gottlieb, Eric Raphael. “Humoral Immune Responses to a Malaria Vaccine Candidate| Towards a Correlate of Vaccine-Induced Protection.” 2012. Web. 23 Jun 2018.
Vancouver:
Gottlieb ER. Humoral Immune Responses to a Malaria Vaccine Candidate| Towards a Correlate of Vaccine-Induced Protection. [Internet] [Thesis]. University of Maryland, Baltimore; 2012. [cited 2018 Jun 23].
Available from: http://pqdtopen.proquest.com/#viewpdf?dispub=1512511.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Gottlieb ER. Humoral Immune Responses to a Malaria Vaccine Candidate| Towards a Correlate of Vaccine-Induced Protection. [Thesis]. University of Maryland, Baltimore; 2012. Available from: http://pqdtopen.proquest.com/#viewpdf?dispub=1512511
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
4.
DeRosa, Megan Sullivan.
Golden Threads| Shared Themes in Holistic Health and Healing Modalities.
Degree: 2013, Prescott College
URL: http://pqdtopen.proquest.com/#viewpdf?dispub=1530859
► There is growing popularity and widespread use of holistic health and healing modalities, which emphasize a mind-body-spirit approach to supporting or restoring well-being and…
(more)
▼ There is growing popularity and widespread use of holistic health and healing modalities, which emphasize a mind-body-spirit approach to supporting or restoring well-being and wholeness. Research shows that individuals who engage in these modalities experience many benefits. Further, these benefits may be enhanced with the combined use of multiple, complementary modalities. The use of multiple holistic health and healing modalities is further supported by the fact that common themes, or thematic threads, exist across many of these modalities. In this thesis, the author attempted to identify these common themes by utilizing Thematic Analysis to specifically explore the modalities of yoga therapy, energetic healing, holistic life coaching, and holistic nutrition. Across these modalities, the author identified five cogent, shared themes—expanding awareness, letting go, accessing inner guidance, moving toward balance, and experiencing empowerment—which suggest overarching themes that may exists across the entire field of holistic health and healing practices.
Subjects/Keywords: Health Sciences, Alternative Medicine; Spirituality
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APA (6th Edition):
DeRosa, M. S. (2013). Golden Threads| Shared Themes in Holistic Health and Healing Modalities. (Thesis). Prescott College. Retrieved from http://pqdtopen.proquest.com/#viewpdf?dispub=1530859
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
DeRosa, Megan Sullivan. “Golden Threads| Shared Themes in Holistic Health and Healing Modalities.” 2013. Thesis, Prescott College. Accessed June 23, 2018.
http://pqdtopen.proquest.com/#viewpdf?dispub=1530859.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
DeRosa, Megan Sullivan. “Golden Threads| Shared Themes in Holistic Health and Healing Modalities.” 2013. Web. 23 Jun 2018.
Vancouver:
DeRosa MS. Golden Threads| Shared Themes in Holistic Health and Healing Modalities. [Internet] [Thesis]. Prescott College; 2013. [cited 2018 Jun 23].
Available from: http://pqdtopen.proquest.com/#viewpdf?dispub=1530859.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
DeRosa MS. Golden Threads| Shared Themes in Holistic Health and Healing Modalities. [Thesis]. Prescott College; 2013. Available from: http://pqdtopen.proquest.com/#viewpdf?dispub=1530859
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
5.
Peeters, Elisabeth.
Investigation of the tableting process in continuous production: influence of feeding and extended dwell time during compression on dependent process variables and tablet properties.
Degree: 2014, Ghent University
URL: http://hdl.handle.net/1854/LU-5775759
► Despite the high quantities of tablets produced daily, a lot of the established processes and formulations are the results of (suboptimal) trial-and-error experiments, contributing to…
(more)
▼ Despite the high quantities of tablets produced daily, a lot of the established processes and formulations are the results of (suboptimal) trial-and-error experiments, contributing to the image that the entire field of pharmaceutical powder technology is more “an art than a science” [1]. Also, a large number of contributing papers to the field date from roughly two or three decades ago. Although the operation mode and press lay-out stayed basically unchanged since its invention, the instrumentation improved largely in terms of quality, accuracy and sensitivity. Moreover, due to the increased competition through generic manufacturing and the shift towards a more patient-centered production, more advanced tablet formulations exhibiting e.g. prolonged, extended, delayed or immediate release profiles have emerged.
This, together with the Process Analytical Technology (PAT) – initiative, proposed by the American Food and Drug Administration (FDA) in 2004, encourages a shift towards a more scientifically based technology. The goal of the initiative is to stimulate the pharmaceutical industry to “design and develop processes that can consistently ensure a predefined quality at the end of the manufacturing process”. By identifying all sources of variation of importance for the product performance and quality, and by increased process understanding and continuous monitoring, quality could be built rather than tested into the product [2]. Together with the International Conference on Harmonization (ICH) Q8 guideline on Pharmaceutical Development, focusing on the Quality-by-Design (QbD) concept, it forms the regulatory framework to catalyze the shift of the pharmaceutical industry towards a redesigning of the current approach [3-5].
Seen in the light of these new movements within the tablet manufacturing area, it appears obvious that there is a need for new knowledge to fund a base for a thorough understanding of the continuous tableting process. This thesis aims to contribute in this large context by the investigation of the tableting process on a high-speed rotary tablet press. The goal was to increase the understanding of the influence of the properties of the starting material and process parameters on dependent process parameters and tablet properties. Since the PAT-initiative stimulates the introduction of new analytical tools to enhance process understanding and product quality, additional analytical chemistry tools, next to the built-in instrumentation were used. Moreover, due to the large amount of data obtained, Design of Experiments (DoE) and Multivariate Data Analysis (MVA) were administered to perform experiments and analyze data in a structured manner. Three main topics were addressed:
- The role of the forced feeder: its influence on the properties of the starting material, on the die-filling process and on the final tablet properties.
- The mechanism of (double) compression: its influence on dependent process parameters and on the final tablet properties.
- The role of additional analytical chemistry tools in a…
Advisors/Committee Members: Remon, Jean Paul, Vervaet, Chris.
Subjects/Keywords: Medicine and Health Sciences
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APA ·
Chicago ·
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CSE |
Export
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Manager
APA (6th Edition):
Peeters, E. (2014). Investigation of the tableting process in continuous production: influence of feeding and extended dwell time during compression on dependent process variables and tablet properties. (Thesis). Ghent University. Retrieved from http://hdl.handle.net/1854/LU-5775759
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Peeters, Elisabeth. “Investigation of the tableting process in continuous production: influence of feeding and extended dwell time during compression on dependent process variables and tablet properties.” 2014. Thesis, Ghent University. Accessed June 23, 2018.
http://hdl.handle.net/1854/LU-5775759.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Peeters, Elisabeth. “Investigation of the tableting process in continuous production: influence of feeding and extended dwell time during compression on dependent process variables and tablet properties.” 2014. Web. 23 Jun 2018.
Vancouver:
Peeters E. Investigation of the tableting process in continuous production: influence of feeding and extended dwell time during compression on dependent process variables and tablet properties. [Internet] [Thesis]. Ghent University; 2014. [cited 2018 Jun 23].
Available from: http://hdl.handle.net/1854/LU-5775759.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Peeters E. Investigation of the tableting process in continuous production: influence of feeding and extended dwell time during compression on dependent process variables and tablet properties. [Thesis]. Ghent University; 2014. Available from: http://hdl.handle.net/1854/LU-5775759
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
6.
Vercruysse, Jurgen.
Innovation in pharmaceutical manufacturing of solid dosage forms via continuous wet granulation.
Degree: 2014, Ghent University
URL: http://hdl.handle.net/1854/LU-5765595
► For decades, the manufacturing of solid dosage forms in the pharmaceutical industry has been synonymous with batch processing. However, batch processes are often sensitive to…
(more)
▼ For decades, the manufacturing of solid dosage forms in the pharmaceutical industry has been synonymous with batch processing. However, batch processes are often sensitive to variability (possibly resulting in batch rejection or reprocessing), high labor costs (due to the multiple manual interventions during processing), high production costs, excessive inventories and scale-up issues. On the other hand, many advantages are associated with continuous processing which are all related to important economic drivers for change (i.e. quality, cost and time). However, implementation of new technologies, methodologies and equipment is needed to allow transfer from batch to continuous manufacturing within the pharmaceutical industry.
As wet granulation is the most popular method to improve material properties (flow, homogeneity, compressibility) prior to tableting, it is obvious that a continuous wet granulation process is of high importance for a manufacturer of solid dosage forms. At the Laboratory of Pharmaceutical Technology (Ghent University) a continuous twin screw wet granulation process using a modified twin-screw extruder was developed. Based on this research work, GEA Pharma Systems developed a fully integrated ‘from-powder-to-tablet’ manufacturing line, the ConsiGmaTM system, using twin screw granulation as an intermediate step for wet granulation of raw materials.
The overall objective of this project was to bridge the gap between the existing technology and its industrial implementation. Although technical know-how about the continuous granulation system is available at the manufacturer of this system, process knowledge (certainly towards specific pharmaceutical formulations) about this innovative manufacturing technique is limited within the pharmaceutical industry. Also in scientific literature the number of papers dealing with continuous wet granulation is limited. Therefore, the continuous granulation of pharmaceuticals by means of the ConsiGmaTM system was investigated in order to (a) improve process knowledge and process understanding of this novel manufacturing tool for solid dosage forms and (b) simplify scale-up and transfers in pharmaceutical manufacturing.
To meet these objectives, the parameters affecting the quality of the end product manufactured via continuous wet granulation using the ConsiGmaTM system were determined via a series of experiments which evaluated the different aspects of this granulation technique: influence of formulation, process and equipment parameters on granule and tablet quality, visualization and understanding of granulation liquid mixing and distribution, use of continuous granulation for formulation development and process optimization, stability and repeatability of the continuous granulation process and in-line blending capacity of the granulation unit.
Advisors/Committee Members: Vervaet, Chris, Remon, Jean Paul.
Subjects/Keywords: Medicine and Health Sciences
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APA ·
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Manager
APA (6th Edition):
Vercruysse, J. (2014). Innovation in pharmaceutical manufacturing of solid dosage forms via continuous wet granulation. (Thesis). Ghent University. Retrieved from http://hdl.handle.net/1854/LU-5765595
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Vercruysse, Jurgen. “Innovation in pharmaceutical manufacturing of solid dosage forms via continuous wet granulation.” 2014. Thesis, Ghent University. Accessed June 23, 2018.
http://hdl.handle.net/1854/LU-5765595.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Vercruysse, Jurgen. “Innovation in pharmaceutical manufacturing of solid dosage forms via continuous wet granulation.” 2014. Web. 23 Jun 2018.
Vancouver:
Vercruysse J. Innovation in pharmaceutical manufacturing of solid dosage forms via continuous wet granulation. [Internet] [Thesis]. Ghent University; 2014. [cited 2018 Jun 23].
Available from: http://hdl.handle.net/1854/LU-5765595.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Vercruysse J. Innovation in pharmaceutical manufacturing of solid dosage forms via continuous wet granulation. [Thesis]. Ghent University; 2014. Available from: http://hdl.handle.net/1854/LU-5765595
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
7.
Degfie, Tizta Tilahun.
Marital dynamics in family planning : the role of couple interaction in Jimma zone, Southwest Ehtiopia.
Degree: 2014, Ghent University
URL: http://hdl.handle.net/1854/LU-5784759
► Introduction : Worldwide, the number of maternal deaths in 2010 amounted to 287,000; almost 90% occurred in developing countries where rural areas typically share the…
(more)
▼ Introduction : Worldwide, the number of maternal deaths in 2010 amounted to 287,000; almost 90% occurred in developing countries where rural areas typically share the highest proportion of it. In Ethiopia, the maternal mortality ratio (MMR) was 676/100,000 live births in 2011, contraceptive prevalence was 28.6% and the unmet need for family planning 25%. This is echoed in the country’s population growth which does not match the country´s development, as illustrated by the poor living condition among women. Since poor access to and low use of contraceptives exist, it is essential for further family planning interventions to ascertain determinant factors of effectiveness in order to obtain a more efficient use of family planning services. In that perspective, some studies have recommended focusing on couple level analysis in fertility research because it gives a better predictive power of relationships between family planning and couple´s fertility desire. The decision to use contraceptive methods is indeed a complex process influenced by several factors. Few studies, however, have identified how the reasons for use or non-use of contraceptives relate to marital relationship.
Objectives and methods : The general objective of this doctoral study was to examine the marital dynamics concerning family planning and to assess the effect that family planning education has on a couple’s interaction and contraceptive use. This family planning education was offered through an intervention program carried out among married couples in Jimma Zone in south-western Ethiopia. For the purpose of this intervention program, we identified three specific objectives.
The first objective was to determine the male-female difference in knowledge, attitude and practices of family planning, as well as the factors that lie at the origin of this difference. Second, we aimed at looking at spousal agreement on fertility desire and spousal communication from both husbands’ and wives’ perspective. For that purpose, we measured concordance in fertility desires between spouses. The third objective was to observe the effect of couple family planning education on 1) husband-wife interaction regarding contraceptive use and 2) male involvement in family planning. This objective mainly conveyed the impact of the family planning intervention of this study.
We recruited 854 couples for our study and collected data using quantitative technique. A quasi-experimental design was used in which couples were divided in a control and intervention arm. For the intervention arm, family planning education focusing on male involvement in family planning was implemented to analyse its effect on couples’ contraceptive practice. A first intervention focused on males, under the assumption that a better awareness of contraceptives by men would help in promoting contraceptives. We educated men about their shared responsibility not only in decision-making about fertility desire but also by involving them in family planning as such. The second set of interventions targeted…
Advisors/Committee Members: Degomme, Olivier, Coene, Gily.
Subjects/Keywords: Medicine and Health Sciences
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APA ·
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CSE |
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to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Degfie, T. T. (2014). Marital dynamics in family planning : the role of couple interaction in Jimma zone, Southwest Ehtiopia. (Thesis). Ghent University. Retrieved from http://hdl.handle.net/1854/LU-5784759
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Degfie, Tizta Tilahun. “Marital dynamics in family planning : the role of couple interaction in Jimma zone, Southwest Ehtiopia.” 2014. Thesis, Ghent University. Accessed June 23, 2018.
http://hdl.handle.net/1854/LU-5784759.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Degfie, Tizta Tilahun. “Marital dynamics in family planning : the role of couple interaction in Jimma zone, Southwest Ehtiopia.” 2014. Web. 23 Jun 2018.
Vancouver:
Degfie TT. Marital dynamics in family planning : the role of couple interaction in Jimma zone, Southwest Ehtiopia. [Internet] [Thesis]. Ghent University; 2014. [cited 2018 Jun 23].
Available from: http://hdl.handle.net/1854/LU-5784759.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Degfie TT. Marital dynamics in family planning : the role of couple interaction in Jimma zone, Southwest Ehtiopia. [Thesis]. Ghent University; 2014. Available from: http://hdl.handle.net/1854/LU-5784759
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
University of South Florida
8.
Billington, Alicia.
Applying Modeled Hemi-Ellipsoids to the Study of Pressure Distribution in Normal and Paraplegic Seated Subjects.
Degree: 2014, University of South Florida
URL: http://scholarcommons.usf.edu/etd/5183
► The three goals of this research were to investigate how normal subjects move while seated, how paraplegic patients move while seated, and whether seated movements…
(more)
▼ The three goals of this research were to investigate how normal subjects move while seated, how paraplegic patients move while seated, and whether seated movements can be modeled using a hemi-ellipsoid shape. Pressure readings were recorded at 11 Hz using a 36 by 36 sensor pressure map by XSENSOR. Subjects were instructed to move or perform pressure relief as they normally would while seated. Analysis was performed using Microsoft Excel with Solver and Matrix.xla add-ins and automated with VBA code. Major movements and time intervals between movements were calculated by locating the area of maximum pressure on each hemi-buttock for 20 normal and 6 paraplegic subjects. Statistical analysis revealed movements followed a normal distribution while time intervals followed a lognormal distribution. For both the normal (p=0.041) and paraplegic groups (p=0.007) the number of movements significantly increased from the first hour of recording to the second hour. The time interval between major movements decreased but not significantly for neither the normal subjects nor the paraplegics. No significant differences were identified between the normal and paraplegic groups over the first hour or second hour for number of movements or time intervals. Time series analysis with plotting, trend lines, ARIMA, and periodograms did not reveal patterns in the data. Preference for a side was shown. Next, all areas of identified major movements for one subject and one frame for each of the paraplegic patients were modeled as a hemi-ellispoid shape using minimization with Solver. Eigenvalues were calculated in order to obtain the lengths of the x, y, and z axis of the hemi-ellipsoid with an average error of 39.87% for the normal subject and an error range of 5.10% to 2701.81% for the paraplegic patients.
Subjects/Keywords: Engineering; Medicine and Health Sciences
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APA (6th Edition):
Billington, A. (2014). Applying Modeled Hemi-Ellipsoids to the Study of Pressure Distribution in Normal and Paraplegic Seated Subjects. (Thesis). University of South Florida. Retrieved from http://scholarcommons.usf.edu/etd/5183
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Billington, Alicia. “Applying Modeled Hemi-Ellipsoids to the Study of Pressure Distribution in Normal and Paraplegic Seated Subjects.” 2014. Thesis, University of South Florida. Accessed June 23, 2018.
http://scholarcommons.usf.edu/etd/5183.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Billington, Alicia. “Applying Modeled Hemi-Ellipsoids to the Study of Pressure Distribution in Normal and Paraplegic Seated Subjects.” 2014. Web. 23 Jun 2018.
Vancouver:
Billington A. Applying Modeled Hemi-Ellipsoids to the Study of Pressure Distribution in Normal and Paraplegic Seated Subjects. [Internet] [Thesis]. University of South Florida; 2014. [cited 2018 Jun 23].
Available from: http://scholarcommons.usf.edu/etd/5183.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Billington A. Applying Modeled Hemi-Ellipsoids to the Study of Pressure Distribution in Normal and Paraplegic Seated Subjects. [Thesis]. University of South Florida; 2014. Available from: http://scholarcommons.usf.edu/etd/5183
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
9.
Oliver, Brant J.
Modifiable Factor Relationships with Biopsychosocial Disability in Multiple Sclerosis.
Degree: 2017, Dartmouth College
URL: http://pqdtopen.proquest.com/#viewpdf?dispub=10184281
► Problem Statement: Multiple sclerosis (MS) is a chronic disease of the central nervous system and is among the most common neurological disorders in adults.…
(more)
▼ Problem Statement: Multiple sclerosis (MS) is a chronic disease of the central nervous system and is among the most common neurological disorders in adults. MS causes significant disability and has no cure. Neurological disease severity is thought to contribute to disability but may not be the main determinant. Individual and environmental factors may modify the relationship between disease severity and disability or directly influence it. Research simultaneously relating these factors in MS has not yet been conducted. Methods: Participants completed an internet questionnaire and a telephone-administered cognitive recall performance task. Clinical data were extracted from a data registry maintained by the Multiple Sclerosis Center at Dartmouth-Hitchcock Medical Center (DHMC) in Lebanon, New Hampshire. Correlation and multiple regression analyses were conducted to determine the relationships between disease severity, individual factors, environmental factors and biopsychosocial disability (BPSD). Results: One hundred-eighty (180) adults with relapsing forms of MS participated in the study, representing approximately 15% of the total MS patient population followed by the database registry at DHMC. The study population was 76% female, had 14.6 years of education, a mean annual income of $35,000, was 75% relapsing-remitting MS (RRMS), had a mean EDSS of 2.4, and a mean ARR of 0.21. In multiple regression analyses, disease severity explained 3%, demographic variables 6%, individual factors 69%, and environmental factors 64% of the variance in BPSD, respectively. The final model incorporating disease severity, individual factors, and environmental factors explained 74% of the variance in BPSD. Depression and environmental status demonstrated the strongest associations with BPSD. Conclusions: Neuropsychiatric status, bowel and bladder function, self-efficacy, social support, and environmental status are key factors associated with BPSD. These factors are modifiable by medical, psychosocial, and/or case management interventions. This study has three major limitations: (1) inability to predict causality or directionality of associations; (2) susceptibility to self-report bias; and (3) susceptibility to temporal bias. This study provides a basis for continued research on biopsychosocial disability reduction and to related healthcare quality improvement, shared decision making, and health policy initiatives.
Subjects/Keywords: Medicine; Health sciences; Nursing
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APA (6th Edition):
Oliver, B. J. (2017). Modifiable Factor Relationships with Biopsychosocial Disability in Multiple Sclerosis. (Thesis). Dartmouth College. Retrieved from http://pqdtopen.proquest.com/#viewpdf?dispub=10184281
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Oliver, Brant J. “Modifiable Factor Relationships with Biopsychosocial Disability in Multiple Sclerosis.” 2017. Thesis, Dartmouth College. Accessed June 23, 2018.
http://pqdtopen.proquest.com/#viewpdf?dispub=10184281.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Oliver, Brant J. “Modifiable Factor Relationships with Biopsychosocial Disability in Multiple Sclerosis.” 2017. Web. 23 Jun 2018.
Vancouver:
Oliver BJ. Modifiable Factor Relationships with Biopsychosocial Disability in Multiple Sclerosis. [Internet] [Thesis]. Dartmouth College; 2017. [cited 2018 Jun 23].
Available from: http://pqdtopen.proquest.com/#viewpdf?dispub=10184281.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Oliver BJ. Modifiable Factor Relationships with Biopsychosocial Disability in Multiple Sclerosis. [Thesis]. Dartmouth College; 2017. Available from: http://pqdtopen.proquest.com/#viewpdf?dispub=10184281
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
University of Tennessee – Knoxville
10.
Bahng, Junghwa.
Acoustic Cue Weighting in Children Wearing Cochlear Implants.
Degree: 2008, University of Tennessee – Knoxville
URL: http://trace.tennessee.edu/utk_graddiss/325
► The purpose of this study was to determine how normal hearing adults (NHA), normal hearing children (NHC) and children wearing cochlear implants (CI) differ in…
(more)
▼ The purpose of this study was to determine how normal hearing adults (NHA), normal hearing children (NHC) and children wearing cochlear implants (CI) differ in the perceptual weight given cues for fricative consonant and voiceless stop consonant continua. Ten normal-hearing adults (NHA), eleven 5-8-year-old normalhearing children (NHC) and eight 5-8-year-old children wearing cochlear implants (CI) were participants. For fricative consonant perception, the /su/-/∫u/ continua were constructed by varying a fricative spectrum cue in three steps and by varying a F2 onset transition cue in three steps. For voiceless stop consonant perception, the /pu/- /tu/ continua were constructed by varying a burst cue in three steps and a F2 onset transition cue in three steps. A quantitative method of analysis (ANOVA model) was used to determine cue weighting and measure cue interaction. For the fricative consonant, both NHC and NHA gave more perceptual weight to the frication spectral cue than to the formant transition. NHC gave significantly less weight to the fricative spectrum cue than NHA. The weight given the transition cue was similar for NHC and NHA, and the degree of cue interaction was similar between two groups. The CI group gave more perceptual weight to the fricative spectrum cue than to the transition. The degree of cue interaction was not significant for CI. For the voiceless stop consonant, both NHC and NHA gave more perceptual weight to the transition cue than to the burst cue. NHC gave proportionately less weight to the transition cue than NHA. The weight given the burst cue and the degree of cue interaction were similar between NHC and NHA. The CI group gave more perceptual weight to the transition cue than to the burst cue, and there was no significant difference between children wearing cochlear implants and normal hearing children group; however, the degree of cue interaction was not significant for CI. These results indicated that all groups favored the longer-duration cue to make phonemic judgments. Also there were developmental patterns. The CI group has similar cue weighting strategies to agematched NHC, but the integration of the cues was not significant for either fricative or voiceless stop consonant perception.
Subjects/Keywords: Medicine and Health Sciences
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APA (6th Edition):
Bahng, J. (2008). Acoustic Cue Weighting in Children Wearing Cochlear Implants. (Doctoral Dissertation). University of Tennessee – Knoxville. Retrieved from http://trace.tennessee.edu/utk_graddiss/325
Chicago Manual of Style (16th Edition):
Bahng, Junghwa. “Acoustic Cue Weighting in Children Wearing Cochlear Implants.” 2008. Doctoral Dissertation, University of Tennessee – Knoxville. Accessed June 23, 2018.
http://trace.tennessee.edu/utk_graddiss/325.
MLA Handbook (7th Edition):
Bahng, Junghwa. “Acoustic Cue Weighting in Children Wearing Cochlear Implants.” 2008. Web. 23 Jun 2018.
Vancouver:
Bahng J. Acoustic Cue Weighting in Children Wearing Cochlear Implants. [Internet] [Doctoral dissertation]. University of Tennessee – Knoxville; 2008. [cited 2018 Jun 23].
Available from: http://trace.tennessee.edu/utk_graddiss/325.
Council of Science Editors:
Bahng J. Acoustic Cue Weighting in Children Wearing Cochlear Implants. [Doctoral Dissertation]. University of Tennessee – Knoxville; 2008. Available from: http://trace.tennessee.edu/utk_graddiss/325
University of Tennessee – Knoxville
11.
Brown, Elizabeth.
The Association Between The Rate of Child Fatalities in Tennessee and Selected Neighborhood Demographic and Housing Characteristics.
Degree: 2008, University of Tennessee – Knoxville
URL: http://trace.tennessee.edu/utk_graddiss/477
► The purpose of this study was to determine if an association exists between housing characteristics and demographic characteristics of Tennessee neighborhoods (census tracts) and the…
(more)
▼ The purpose of this study was to determine if an association exists between housing characteristics and demographic characteristics of Tennessee neighborhoods (census tracts) and the rate of child fatalities (violent, accidental, and natural) in the neighborhoods reporting child fatalities for the years 1996-2003. The child fatalities, for the eight years, reported to each Tennessee Judicial District Child Fatality Review Team required by the Tennessee Department of Health’s Maternal and Child Health Division were selected for use in the study. Data was selected from the Bureau of the Census’ 2000 United States Census to obtain the housing characteristics and demographic characteristics of heads of households by census tract. The data were analyzed using descriptive statistics, Spearman Rho, and Chi-Square Cross-tabulation analyses.
The descriptive profile showed the under one year of age had the most child fatalities for the eight years. The children aged 15 - 18 years old were the second largest group of child fatalities for the years 1996-2003. Children aged one to four years made up the third largest group of fatalities in Tennessee for the years 1996-2003. More male children died than female children in Tennessee from 1996-2003. The top six most frequently reported causes of death were illness or other natural causes, prematurity, vehicular, Sudden Infant Death Syndrome (SIDS), and firearm fatalities. In Tennessee, the top two reported manners of death were natural and accidental. The following conclusions were drawn from the findings of the study; the neighborhood housing characteristics of the percent of rental housing and household size was associated with the rate of Tennessee child fatalities classified as violent and accidental deaths; the neighborhood demographic characteristic of Non-white heads of households was associated with the rate of Tennessee violent and natural child fatalities; and the neighborhood housing characteristics of the percent of rental housing, vacancy status, household size, and urban location were associated with the rate of Tennessee child fatalities classified as natural deaths. More research needs to be conducted to determine the nature of the weak associations between the neighborhood housing and demographic characteristics and the rate of child fatalities.
Subjects/Keywords: Medicine and Health Sciences
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APA (6th Edition):
Brown, E. (2008). The Association Between The Rate of Child Fatalities in Tennessee and Selected Neighborhood Demographic and Housing Characteristics. (Doctoral Dissertation). University of Tennessee – Knoxville. Retrieved from http://trace.tennessee.edu/utk_graddiss/477
Chicago Manual of Style (16th Edition):
Brown, Elizabeth. “The Association Between The Rate of Child Fatalities in Tennessee and Selected Neighborhood Demographic and Housing Characteristics.” 2008. Doctoral Dissertation, University of Tennessee – Knoxville. Accessed June 23, 2018.
http://trace.tennessee.edu/utk_graddiss/477.
MLA Handbook (7th Edition):
Brown, Elizabeth. “The Association Between The Rate of Child Fatalities in Tennessee and Selected Neighborhood Demographic and Housing Characteristics.” 2008. Web. 23 Jun 2018.
Vancouver:
Brown E. The Association Between The Rate of Child Fatalities in Tennessee and Selected Neighborhood Demographic and Housing Characteristics. [Internet] [Doctoral dissertation]. University of Tennessee – Knoxville; 2008. [cited 2018 Jun 23].
Available from: http://trace.tennessee.edu/utk_graddiss/477.
Council of Science Editors:
Brown E. The Association Between The Rate of Child Fatalities in Tennessee and Selected Neighborhood Demographic and Housing Characteristics. [Doctoral Dissertation]. University of Tennessee – Knoxville; 2008. Available from: http://trace.tennessee.edu/utk_graddiss/477
University of Tennessee – Knoxville
12.
Sarangi, Pranita Pragnyadipta.
Induction and Regulation of Herpetic Stromal Keratitis.
Degree: 2008, University of Tennessee – Knoxville
URL: http://trace.tennessee.edu/utk_graddiss/344
► Herpetic stromal keratitis (HSK) is an immunopathological and tissue destructive corneal lesion caused by herpes simplex virus (HSV) infection, which induces an intense inflammatory response…
(more)
▼ Herpetic stromal keratitis (HSK) is an immunopathological and tissue destructive corneal lesion caused by herpes simplex virus (HSV) infection, which induces an intense inflammatory response and finally leads to blindness. Accumulating evidence using the murine model has shown that Th-1 phenotype CD4+ T cells orchestrating the inflammation mainly contribute to the immunopathological reaction in HSV-1 infected cornea. However, prior to CD4+ T cell infiltration into corneal lesions, various innate immune cells recruit and produce numerous inflammatory and angiogenic molecules into the corneal stroma those in turn drive the corneal immunopathology.
The first part (Part I) of this dissertation focuses on the understanding of HSV-1 induced immunoinflammatory processes in the cornea and trigeminal ganglia including the secondary lymphoid tissues. The next three parts (Part II-IV) focus on different inflammatory and anti-inflammatory mechanisms that are activated following virus infection in the cornea. Results in Part II evaluate the role of various Toll-like receptors (TLRs) in driving the early inflammatory events that occur in the HSV infected corneas. Thus, this part demonstrates that mainly TLR2 and to a lesser extent TLR9 ligand activity of HSV is important for driving SK pathology. Results of the third section show that HSV infection results in the up regulation of IL-23 that is needed for the survival of pathogenic Th-17 cells. The data show that mice lacking IL-23 were more susceptible to SK lesions and that the heightened lesion severity was attributed to higher IL-12 production and Th1 immune response in such animals. The fourth section describes the relative role of IL-10 and natural regulatory T cells (nTregs) as two independent anti-inflammatory mechanisms that are needed for controlling HSK lesions.
In this study, experiments were designed to understand the mechanisms involved in the induction and regulation of immunopathology in HSK and modulation of such processes could be useful in designing therapeutic for HSK.
Subjects/Keywords: Medicine and Health Sciences
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APA (6th Edition):
Sarangi, P. P. (2008). Induction and Regulation of Herpetic Stromal Keratitis. (Doctoral Dissertation). University of Tennessee – Knoxville. Retrieved from http://trace.tennessee.edu/utk_graddiss/344
Chicago Manual of Style (16th Edition):
Sarangi, Pranita Pragnyadipta. “Induction and Regulation of Herpetic Stromal Keratitis.” 2008. Doctoral Dissertation, University of Tennessee – Knoxville. Accessed June 23, 2018.
http://trace.tennessee.edu/utk_graddiss/344.
MLA Handbook (7th Edition):
Sarangi, Pranita Pragnyadipta. “Induction and Regulation of Herpetic Stromal Keratitis.” 2008. Web. 23 Jun 2018.
Vancouver:
Sarangi PP. Induction and Regulation of Herpetic Stromal Keratitis. [Internet] [Doctoral dissertation]. University of Tennessee – Knoxville; 2008. [cited 2018 Jun 23].
Available from: http://trace.tennessee.edu/utk_graddiss/344.
Council of Science Editors:
Sarangi PP. Induction and Regulation of Herpetic Stromal Keratitis. [Doctoral Dissertation]. University of Tennessee – Knoxville; 2008. Available from: http://trace.tennessee.edu/utk_graddiss/344
University of Tennessee – Knoxville
13.
Choudhary, Shambhunath.
Histone Deacetylase Inhibitors for Selective Anti-Cancer Therapeutics.
Degree: 2009, University of Tennessee – Knoxville
URL: http://trace.tennessee.edu/utk_graddiss/27
► Activating mutations of ras genes are frequently found in human cancers. Since Ras proteins and their functions play an important role in tumorigenesis, it is…
(more)
▼ Activating mutations of ras genes are frequently found in human cancers. Since Ras proteins and their functions play an important role in tumorigenesis, it is important to develop targeted anticancer therapeutics against Ras-related human cancers. We observed that in addition to tumorigenic ability, oncogenic H-Ras possesses a novel proapoptotic ability to facilitate the induction of apoptosis by histone deacetylase inhibitors (HDACIs), such as FR901228 and trichostatin A (TSA). HDACIs make up a new class of structurally diverse anticancer agents and have been shown to exhibit antimetastatic and antiangiogenic activities toward malignantly transformed cells. We detected that expression of oncogenic H-Ras potentiated intracellular reactive oxygen species (ROS) in human and mouse cells to enhance HDACI-induced ROS, thereby contributing to the induction of selective apoptosis and caspase activation.
The first part (Part I) of this dissertation focuses on the understanding of Ras proteins, their role in normal and transformed cell physiology, and current treatment options against Ras-related human cancers, as well as the role of HDACIs and ROS in anticancer therapeutics. The next three parts (Part II-IV) focus on revealing the mechanisms for the novel pro-apoptotic ability of oncogenic H-Ras that allow HDACIs to induce selective apoptosis of the oncogenic H-Ras expressing cells. Results in Part II & III verify the pro-apoptotic activity of oncogenic H-Ras in the increased susceptibility of human cancer cells to HDACIs. The caspase pathways, the B-Raf and extracellular signal regulated kinase pathway, p21Cip1 and p27Kip1, and core histone contents are regulated differently by FR901228 in oncogenic H-Ras–expressed cells than their counterparts in parental cells, contributing to the increased susceptibility to the induction of selective apoptosis. Results in Part IV describe the role of reactive oxygen species in the pro-apoptotic ability ofoncogenic H-Ras to enhance the cell susceptibility to HDACIs. Intracellular ROS was cooperatively up-regulated by oncogenc H-Ras and HDACI treatment to induce selective apoptosis of oncogenic H-Ras-expressing cells. The last section (Part V) summarizes the findings with their importance and discusses future directions.
Subjects/Keywords: Other Medicine and Health Sciences
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APA (6th Edition):
Choudhary, S. (2009). Histone Deacetylase Inhibitors for Selective Anti-Cancer Therapeutics. (Doctoral Dissertation). University of Tennessee – Knoxville. Retrieved from http://trace.tennessee.edu/utk_graddiss/27
Chicago Manual of Style (16th Edition):
Choudhary, Shambhunath. “Histone Deacetylase Inhibitors for Selective Anti-Cancer Therapeutics.” 2009. Doctoral Dissertation, University of Tennessee – Knoxville. Accessed June 23, 2018.
http://trace.tennessee.edu/utk_graddiss/27.
MLA Handbook (7th Edition):
Choudhary, Shambhunath. “Histone Deacetylase Inhibitors for Selective Anti-Cancer Therapeutics.” 2009. Web. 23 Jun 2018.
Vancouver:
Choudhary S. Histone Deacetylase Inhibitors for Selective Anti-Cancer Therapeutics. [Internet] [Doctoral dissertation]. University of Tennessee – Knoxville; 2009. [cited 2018 Jun 23].
Available from: http://trace.tennessee.edu/utk_graddiss/27.
Council of Science Editors:
Choudhary S. Histone Deacetylase Inhibitors for Selective Anti-Cancer Therapeutics. [Doctoral Dissertation]. University of Tennessee – Knoxville; 2009. Available from: http://trace.tennessee.edu/utk_graddiss/27
University of Tennessee – Knoxville
14.
Dziduszko, Agnieszka.
Coronaviral 2’-O-Methyltransferase Function in Virus Replication.
Degree: 2009, University of Tennessee – Knoxville
URL: http://trace.tennessee.edu/utk_graddiss/36
► Primary and higher order RNA structures in the 5’ and 3’ untranslated regions of the genomes of positive-strand RNA viruses are known to function as…
(more)
▼ Primary and higher order RNA structures in the 5’ and 3’ untranslated regions of the genomes of positive-strand RNA viruses are known to function as cis-acting elements for genome translation and replication. Four cis-acting replication elements have been identified in the bovine coronavirus (BCoV) 3’ untranslated region that are postulated to recruit viral and cellular proteins for assembly of the replication complex and initiation of negative-strand synthesis. This study had the goal of identifying and establishing the function of the viral proteins that bind BCoV 3’ terminal cis-acting RNA structures. A major surprising discovery was a specific interaction of the BCoV-encoded 2’-O-methyltransferase (2’-O-MT), an enzyme normally involved in 5’-terminal cap methylation, with a 3’-terminal cis-acting octamerassociated bulged stem-loop. This discovery led to detailed analyses of BCoV 2’-O-MT that is summarized as follows: (i) Enzyme probing revealed the higher-order structure of the cis-acting 3’-terminal bulged stem loop. (ii) Specific binding of the 2’-O-MT to both the 5’ cap-0 structure and to the 3’-terminal bulged stem-loop was documented. (iii) Enhanced translation of a viral genomic RNA was demonstrated as a function of 2’-O-MT methylating activity. (iv) A requirement for 2’-O-MT methylating activity for virus replication was demonstrated with the use of the mouse hepatitis coronavirus reverse genetics system. (v) Over-expression of the viral 2’-O-MT in virus-infected cells had no detrimental in-trans effect on viral genome replication, transcription, or translation, but affected DI RNA accumulation in-cis. These results indicate that the coronaviral 2’-O-MT is essential for virus genome translation and replication and, as a multifunctional protein, is an attractive target for development of an anticoronaviral therapeutic drug.
Subjects/Keywords: Medicine and Health Sciences
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APA (6th Edition):
Dziduszko, A. (2009). Coronaviral 2’-O-Methyltransferase Function in Virus Replication. (Doctoral Dissertation). University of Tennessee – Knoxville. Retrieved from http://trace.tennessee.edu/utk_graddiss/36
Chicago Manual of Style (16th Edition):
Dziduszko, Agnieszka. “Coronaviral 2’-O-Methyltransferase Function in Virus Replication.” 2009. Doctoral Dissertation, University of Tennessee – Knoxville. Accessed June 23, 2018.
http://trace.tennessee.edu/utk_graddiss/36.
MLA Handbook (7th Edition):
Dziduszko, Agnieszka. “Coronaviral 2’-O-Methyltransferase Function in Virus Replication.” 2009. Web. 23 Jun 2018.
Vancouver:
Dziduszko A. Coronaviral 2’-O-Methyltransferase Function in Virus Replication. [Internet] [Doctoral dissertation]. University of Tennessee – Knoxville; 2009. [cited 2018 Jun 23].
Available from: http://trace.tennessee.edu/utk_graddiss/36.
Council of Science Editors:
Dziduszko A. Coronaviral 2’-O-Methyltransferase Function in Virus Replication. [Doctoral Dissertation]. University of Tennessee – Knoxville; 2009. Available from: http://trace.tennessee.edu/utk_graddiss/36
University of Tennessee – Knoxville
15.
Joo, Hye Mee.
Quantitative Analysis of Influenza Virus-Specific B Cell Responses Generated by Infection and Vaccination.
Degree: 2009, University of Tennessee – Knoxville
URL: http://trace.tennessee.edu/utk_graddiss/52
► The two cellular components of B cell memory are antibody (Ab)-secreting cells (ASCs) and memory B cells (BMem). BMem are quiescent cells that respond to…
(more)
▼ The two cellular components of B cell memory are antibody (Ab)-secreting cells (ASCs) and memory B cells (BMem). BMem are quiescent cells that respond to “recall” antigen and contribute substantially to vaccine effectiveness. The rational evaluation of vaccination strategies is dependent on quantitative information on the state of B cell memory. Despite the importance of BMem in resistance to infection, there is little information on the nature of BMem populations generated by influenza infection or vaccination. The major goal of this dissertation was a comprehensive quantitative analysis of the frequency and distribution of influenza-specific BMem generated by influenza infection of the respiratory tract, or by vaccination with inactivated virus.
To achieve this, a prerequisite was the development of a sensitive and reproducible limiting dilution assay (LDA) for determining influenza-specific BMem frequencies. The results in Chapter 2 demonstrated that a strategy utilizing BPL-inactivated virus particles was effective for the in vitro activation of virus-specific BMem. This strategy selectively activates virus-specific BMem and, importantly, achieves this without a requirement for CD4+ T cell associated factors.
Using this strategy, the frequency of Influenza specific BMem in different anatomical sites was measured and we also tested three different forms of influenza virus immunization strategy; (i) intranasal (i.n.) infection, (ii) i.n. vaccination, (iii) intramuscular (i.m.) vaccination. The results in Chapter III, IV showed that following different forms of immunization, BMem dispersed broadly to organized lymphoid tissues throughout the body, and a population of ASCs was established in the bone marrow. ASC and BMem frequencies in these locations reflected the magnitude of the primary B cell response to the form of immunization. Thus, the frequencies were higher following i.n. infection. Interestingly, the state of B cell memory in the lung was profoundly influenced by the form of immunization. The lung was a preferential site of BMem localization after i.n. infection. However, this was not the case after i.m. immunization when BMem frequencies in the lung were considerably lower than in other sites. Our findings point to an effect of influenza infection on the lung environment that profoundly influences ASC and BMem trafficking to this site.
Subjects/Keywords: Medicine and Health Sciences
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APA (6th Edition):
Joo, H. M. (2009). Quantitative Analysis of Influenza Virus-Specific B Cell Responses Generated by Infection and Vaccination. (Doctoral Dissertation). University of Tennessee – Knoxville. Retrieved from http://trace.tennessee.edu/utk_graddiss/52
Chicago Manual of Style (16th Edition):
Joo, Hye Mee. “Quantitative Analysis of Influenza Virus-Specific B Cell Responses Generated by Infection and Vaccination.” 2009. Doctoral Dissertation, University of Tennessee – Knoxville. Accessed June 23, 2018.
http://trace.tennessee.edu/utk_graddiss/52.
MLA Handbook (7th Edition):
Joo, Hye Mee. “Quantitative Analysis of Influenza Virus-Specific B Cell Responses Generated by Infection and Vaccination.” 2009. Web. 23 Jun 2018.
Vancouver:
Joo HM. Quantitative Analysis of Influenza Virus-Specific B Cell Responses Generated by Infection and Vaccination. [Internet] [Doctoral dissertation]. University of Tennessee – Knoxville; 2009. [cited 2018 Jun 23].
Available from: http://trace.tennessee.edu/utk_graddiss/52.
Council of Science Editors:
Joo HM. Quantitative Analysis of Influenza Virus-Specific B Cell Responses Generated by Infection and Vaccination. [Doctoral Dissertation]. University of Tennessee – Knoxville; 2009. Available from: http://trace.tennessee.edu/utk_graddiss/52
University of Tennessee – Knoxville
16.
Lusby, Angela Lea.
Characterization of Feline Adiponectin and its Association with Metabolic Indices in Lean and Obese Cats.
Degree: 2009, University of Tennessee – Knoxville
URL: http://trace.tennessee.edu/utk_graddiss/72
► Adipose tissue secretes over 100 different proteins and cytokines called adipokines. Adiponectin is one of the most intriguing adipokines because it is closely associated with…
(more)
▼ Adipose tissue secretes over 100 different proteins and cytokines called adipokines. Adiponectin is one of the most intriguing adipokines because it is closely associated with insulin sensitivity and is an early marker for Type-2 diabetes mellitus in human beings. Cats are at risk for developing Type-2 diabetes with obesity, so it is important for researchers to understand the role adipokines, like adiponectin, play in feline metabolism. This project sought to lay the foundation for future research regarding feline adiponectin by sequencing adiponectin cDNA, measuring adiponectin’s expression in various tissues, validating and developing new techniques for measuring adiponectin in circulation, determining the influence of gender on adiponectin concentrations, and by monitoring changes in adiponectin as cats gain and lose fat mass.
The results of this project revealed that adiponectin has a similar cDNA and amino acid sequence to other species and is secreted almost exclusively from adipose tissue. However, cats differed from other species in that visceral adipose tissue had more adiponectin expression than subcutaneous adipose tissue. Serum feline adiponectin was measured using a commercially available murine adiponectin ELISA, and the high molecular weight (HMW) form was detected using gel chromatography combined with ELISA. Neutered male cats may have lower concentrations of total adiponectin than female cats and lower percentages of HMW adiponectin than all other gender groups. Similar to humans, HMW adiponectin was more closely associated with improved glucose metabolism than total adiponectin. HMW adiponectin also correlated more closely with body fat mass than total adiponectin. Despite some unique characteristics, feline adiponectin appears similar in structure and function to adiponectin in other species.
Subjects/Keywords: Medicine and Health Sciences
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APA (6th Edition):
Lusby, A. L. (2009). Characterization of Feline Adiponectin and its Association with Metabolic Indices in Lean and Obese Cats. (Doctoral Dissertation). University of Tennessee – Knoxville. Retrieved from http://trace.tennessee.edu/utk_graddiss/72
Chicago Manual of Style (16th Edition):
Lusby, Angela Lea. “Characterization of Feline Adiponectin and its Association with Metabolic Indices in Lean and Obese Cats.” 2009. Doctoral Dissertation, University of Tennessee – Knoxville. Accessed June 23, 2018.
http://trace.tennessee.edu/utk_graddiss/72.
MLA Handbook (7th Edition):
Lusby, Angela Lea. “Characterization of Feline Adiponectin and its Association with Metabolic Indices in Lean and Obese Cats.” 2009. Web. 23 Jun 2018.
Vancouver:
Lusby AL. Characterization of Feline Adiponectin and its Association with Metabolic Indices in Lean and Obese Cats. [Internet] [Doctoral dissertation]. University of Tennessee – Knoxville; 2009. [cited 2018 Jun 23].
Available from: http://trace.tennessee.edu/utk_graddiss/72.
Council of Science Editors:
Lusby AL. Characterization of Feline Adiponectin and its Association with Metabolic Indices in Lean and Obese Cats. [Doctoral Dissertation]. University of Tennessee – Knoxville; 2009. Available from: http://trace.tennessee.edu/utk_graddiss/72
Virginia Commonwealth University
17.
MISTRY, SHRENI.
Characterization of potential interactions between transferrin binding proteins in Neisseria gonorrhoeae.
Degree: PhD, Microbiology & Immunology, 2013, Virginia Commonwealth University
URL: https://scholarscompass.vcu.edu/etd/2954
► Neisseria gonorrhoeae requires iron for survival and establishment of infection in the human host. Pathogenic Neisseriae have evolved a repertoire of high-affinity iron acquisition systems…
(more)
▼ Neisseria gonorrhoeae requires iron for survival and establishment of infection in the human host. Pathogenic Neisseriae have evolved a repertoire of high-affinity iron acquisition systems to facilitate iron uptake in the human host. This requires specific outer membrane receptors and energy-harnessing cytoplasmic membrane proteins. The transferrin receptor proteins of Neisseria gonorrhoeae are necessary for iron uptake from transferrin in the host. The iron uptake system consists of two transferrin binding proteins, (Tbp) A and B. TbpA is an integral outer membrane, TonB-dependent transporter that forms the pore for iron internalization. TbpB is a surface exposed lipoprotein that makes the iron internalization process more efficient.
TbpA is proposed to consist of two distinct domains: a β barrel and an N-terminal plug domain. Previous studies have shown that the EIEYE sequence in the TbpA plug domain plays an important role in iron internalization. We undertook a collaborative project to test the hypothesis that the conserved EIEYE sequence in the wild-type TbpA plug binds Fe3+ during the outer membrane iron transport process. CD spectra analysis and fluorescence emission titration spectra of purified recombinant wildtype and mutated plug proteins revealed that Fe3+ is sequestered by the wildtype TbpA plug protein, unlike the mutated plug protein. Modeling data with the wild-type plug predicts the EIEYE sequence is part of a flexible loop structure and acts as an Fe3+ binding site.
Characterization of the Tbps constituting the gonococcal receptor is important to understanding how the gonococcus survives within its host. TbpA and TbpB act together to acquire iron from human transferrin. We hypothesize that the presence of TbpA impacts the exposure or conformation of TbpB. In this study, we have utilized photoactivable cross-linkers to assess the effect of TbpA on TbpB in live gonococcal cells and studied it in presence of ligand and TonB derived energy. We employed insertion mutants, in which TbpA and TbpB contained the hemagglutinin (HA) epitope tag, to probe for impact of TbpA on TbpB. Our results demonstrate that photo-cross-linking altered TbpB size and migration and was dependent on the presence of TbpA. HA epitope insertion mutants in surface exposed loops of TbpA and TbpB did not impact the mobility of cross-linked TbpB. Addition of human transferrin to the de-energized mutant caused a change in TbpB migration after cross-linking. This result indicates that when ligand is bound tightly and irreversibly to de-energized TbpA, the surface accessibility and perhaps conformation of TbpB is altered and TbpA does not interact with TbpB. Our findings were confirmed with recent structural studies of TbpA-TbpB-ligand triple complex, which illustrate that Tbps bind ligand through unique, non-overlapping binding sites such that TbpA and TbpB do not interact.
TbpB is not an essential member of transferrin-iron acquisition pathway. It is surface exposed and tethered to the outer membrane by a lipid moiety. The role of…
Advisors/Committee Members: CYNTHIA CORNELISSEN.
Subjects/Keywords: Medicine and Health Sciences
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APA (6th Edition):
MISTRY, S. (2013). Characterization of potential interactions between transferrin binding proteins in Neisseria gonorrhoeae. (Doctoral Dissertation). Virginia Commonwealth University. Retrieved from https://scholarscompass.vcu.edu/etd/2954
Chicago Manual of Style (16th Edition):
MISTRY, SHRENI. “Characterization of potential interactions between transferrin binding proteins in Neisseria gonorrhoeae.” 2013. Doctoral Dissertation, Virginia Commonwealth University. Accessed June 23, 2018.
https://scholarscompass.vcu.edu/etd/2954.
MLA Handbook (7th Edition):
MISTRY, SHRENI. “Characterization of potential interactions between transferrin binding proteins in Neisseria gonorrhoeae.” 2013. Web. 23 Jun 2018.
Vancouver:
MISTRY S. Characterization of potential interactions between transferrin binding proteins in Neisseria gonorrhoeae. [Internet] [Doctoral dissertation]. Virginia Commonwealth University; 2013. [cited 2018 Jun 23].
Available from: https://scholarscompass.vcu.edu/etd/2954.
Council of Science Editors:
MISTRY S. Characterization of potential interactions between transferrin binding proteins in Neisseria gonorrhoeae. [Doctoral Dissertation]. Virginia Commonwealth University; 2013. Available from: https://scholarscompass.vcu.edu/etd/2954
Virginia Commonwealth University
18.
Endisha, Helal.
SIRT6 and Premature Aging of Hutchinson-Gilford Progeria Syndrome Fibroblasts.
Degree: MS, Microbiology & Immunology, 2013, Virginia Commonwealth University
URL: https://scholarscompass.vcu.edu/etd/3238
► The genetic disease Hutchinson-Gilford Progeria Syndrome (HGPS) arises from a de novo single nucleotide mutation (1824CàT) in the LMNA gene. As a result, the mutated…
(more)
▼ The genetic disease Hutchinson-Gilford Progeria Syndrome (HGPS) arises from a de novo single nucleotide mutation (1824CàT) in the LMNA gene. As a result, the mutated lamin A protein (progerin) remains farnesylated and permanently attached to the nuclear membrane. Progerin accumulates and deforms the nuclear membrane leading to an array of cellular abnormalities driving the cells to enter a state of permanent cell-cycle arrest early on in replicative age i.e. premature cellular senescence. Cellular senescence has been extensively studied as one of the contributing factors to aging in HGPS patients and other age-related diseases. There has also been evidence to show that aging is accompanied by epigenetic changes and that epigenetic manipulation can incite progeroid syndromes in mice. It has been found in this study that HGPS fibroblasts express distinctly lower levels of SIRT6, a member of the sirtuin family of NAD-dependent protein deacetylases/ADP-ribosyltransferases, than normal fibroblasts. Findings from this study demonstrate that overexpression of SIRT6 prevents a decrease in replicative capacity and the onset of premature senescence in HGPS fibroblasts. Thus, SIRT6 may have promising therapeutic implications for improving HGPS age-related pathologies.
Advisors/Committee Members: Lynne Elmore.
Subjects/Keywords: Medicine and Health Sciences
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APA (6th Edition):
Endisha, H. (2013). SIRT6 and Premature Aging of Hutchinson-Gilford Progeria Syndrome Fibroblasts. (Thesis). Virginia Commonwealth University. Retrieved from https://scholarscompass.vcu.edu/etd/3238
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Endisha, Helal. “SIRT6 and Premature Aging of Hutchinson-Gilford Progeria Syndrome Fibroblasts.” 2013. Thesis, Virginia Commonwealth University. Accessed June 23, 2018.
https://scholarscompass.vcu.edu/etd/3238.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Endisha, Helal. “SIRT6 and Premature Aging of Hutchinson-Gilford Progeria Syndrome Fibroblasts.” 2013. Web. 23 Jun 2018.
Vancouver:
Endisha H. SIRT6 and Premature Aging of Hutchinson-Gilford Progeria Syndrome Fibroblasts. [Internet] [Thesis]. Virginia Commonwealth University; 2013. [cited 2018 Jun 23].
Available from: https://scholarscompass.vcu.edu/etd/3238.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Endisha H. SIRT6 and Premature Aging of Hutchinson-Gilford Progeria Syndrome Fibroblasts. [Thesis]. Virginia Commonwealth University; 2013. Available from: https://scholarscompass.vcu.edu/etd/3238
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Virginia Commonwealth University
19.
Chalasani, Sri Lakshmi.
TOLERANCE OF THYMINE GLYCOL AT THE BLUNT DNA DOUBLE STRAND BREAK FOR NONHOMOLOGUS END JOINING REPAIR AND INTERFERENCE BY BASE EXCISION REPAIR.
Degree: MS, Pharmacology & Toxicology, 2015, Virginia Commonwealth University
URL: https://scholarscompass.vcu.edu/etd/3921
► Radiotherapy is the clinical application of the ionizing radiation to treat cancer. Ionizing radiation causes multiple modes of damage to the DNA damage such…
(more)
▼ Radiotherapy is the clinical application of the ionizing radiation to treat cancer. Ionizing radiation causes multiple modes of damage to the DNA damage such as SSBs, DSBs and modified bases such as thymine glycol. These lesions can exist as clusters in one or two helical turns of DNA. DNA double-strand breaks (DSBs) are extremely toxic to cells because they can lead to genomic rearrangements and even cell death. If base lesions accompany these DSBs, there will be a substantial hindrance for repair.
NHEJ is the primary DSB repair pathway in mammalian cells. HRR repairs single strand breaks (SSBs) or Double strand breaks (DSBs), during late S phase and G2 phase of the cell cycle, by using an undamaged copy of the DNA sequence, and is therefore largely error-free. The NHEJ pathway repairs DSBs without the requirement for sequence homology and can be error-free or error-prone, and is most active during G1 phase.
Thymine glycol (Tg) is the most common oxidation product of thymine. It is produced endogenously as a consequence of aerobic metabolism or via exogenous factors such as ionizing radiation (IR); it is one of the predominant types of base modifications produced by ionizing radiation. Due to clustering of radiation-induced damages, many DSBs are accompanied by damaged bases such as Tg at or near the DSB ends that may interfere with subsequent gap filling and ligation. The base excision repair pathway plays a major role in removal of thymine glycol from the damaged DNA strand. During NHEJ, after synapsis by Ku and DNAPKcs and processing of the DNA ends, XRCC4/Ligase IV complex ligates the DNA. This ligase activity is promoted by the interaction of XLF/XRCC4 filament with Ligase IV.
Linearized plasmids with Tg at the 5th -Tg5 positions from the broken end were subjected to a repair assay using XRCC4-like factor (XLF)-deficient cell extracts, with or without the addition of XLF and Endonuclease III and/or ddTTP and Klenow fragments. End joining of Tg5 was compared to plasmid with Tg at third position-Tg3 in extracts. In addition, the ability of purified NHEJ proteins Ku, DNAPKcs and XRCC4/Ligase IV, to repair the Tg1 (Tg at the end), Tg2 (Tg at the second position), Tg3 and Tg5 in the presence and absence of XLF was assessed.
The data indicated that the cell extract could ligate the Tg5 plasmids only in the presence of XLF. End joining of the Tg5 was less in comparison to Tg3 with base excision repair being more active in Tg5 competing with the joining. Plasmids with Tg were treated with Endonuclease III and ddTTP to test whether the end joining occurred before or after Tg removal.
Endonuclease III and ddTTP treatment showed reduced intensity of the joined fragment suggesting that end joining occurred without removal of Tg in some cases. The extracts were not able to fill in the processed end by the BER though it has a 3′-OH which was filled in by the treatment with Klenow enzymes derived from E.coli DNA Polymerase I, following removal of extract proteins by…
Advisors/Committee Members: Lawrence F Povirk.
Subjects/Keywords: Medicine and Health Sciences
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APA (6th Edition):
Chalasani, S. L. (2015). TOLERANCE OF THYMINE GLYCOL AT THE BLUNT DNA DOUBLE STRAND BREAK FOR NONHOMOLOGUS END JOINING REPAIR AND INTERFERENCE BY BASE EXCISION REPAIR. (Thesis). Virginia Commonwealth University. Retrieved from https://scholarscompass.vcu.edu/etd/3921
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Chalasani, Sri Lakshmi. “TOLERANCE OF THYMINE GLYCOL AT THE BLUNT DNA DOUBLE STRAND BREAK FOR NONHOMOLOGUS END JOINING REPAIR AND INTERFERENCE BY BASE EXCISION REPAIR.” 2015. Thesis, Virginia Commonwealth University. Accessed June 23, 2018.
https://scholarscompass.vcu.edu/etd/3921.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Chalasani, Sri Lakshmi. “TOLERANCE OF THYMINE GLYCOL AT THE BLUNT DNA DOUBLE STRAND BREAK FOR NONHOMOLOGUS END JOINING REPAIR AND INTERFERENCE BY BASE EXCISION REPAIR.” 2015. Web. 23 Jun 2018.
Vancouver:
Chalasani SL. TOLERANCE OF THYMINE GLYCOL AT THE BLUNT DNA DOUBLE STRAND BREAK FOR NONHOMOLOGUS END JOINING REPAIR AND INTERFERENCE BY BASE EXCISION REPAIR. [Internet] [Thesis]. Virginia Commonwealth University; 2015. [cited 2018 Jun 23].
Available from: https://scholarscompass.vcu.edu/etd/3921.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Chalasani SL. TOLERANCE OF THYMINE GLYCOL AT THE BLUNT DNA DOUBLE STRAND BREAK FOR NONHOMOLOGUS END JOINING REPAIR AND INTERFERENCE BY BASE EXCISION REPAIR. [Thesis]. Virginia Commonwealth University; 2015. Available from: https://scholarscompass.vcu.edu/etd/3921
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Virginia Commonwealth University
20.
Damle, Priyadarshan.
Helper phage capsid size redirection by staphylococcal pathogenicity island SaPI1 involves internal scaffolding proteins.
Degree: PhD, Microbiology & Immunology, 2011, Virginia Commonwealth University
URL: https://scholarscompass.vcu.edu/etd/255
► Staphylococcus aureus is one of the leading causes of nosocomial and community acquired infections. Many of the virulence factors are encoded on staphylococcal mobile genetic…
(more)
▼ Staphylococcus aureus is one of the leading causes of nosocomial and community acquired infections. Many of the virulence factors are encoded on staphylococcal mobile genetic elements. Members of the SaPI family of S. aureus mobile elements encode superantigens and are mobilized at high frequency by specific helper bacteriophages. One remarkable feature of helper phage exploitation by SaPIs is remodeling of the normal T=7 bacteriophage capsid to produce smaller T=4 phage-like particles. These particles, composed entirely of helper phage proteins, can accommodate the smaller SaPI genome while excluding that of a complete helper phage. This study was designed to understand the mechanism of capsid size redirection and high frequency mobilization of SaPIs. A multipronged approach employing cryo-EM analysis, protein profile comparison and genetic analysis was used to study the capsid size redirection. Two proteins encoded by the prototype element SaPI1, gp6 and gp7, have been identified in SaPI1 procapsids but not in mature SaPI1 particles. These proteins are sufficient and required to direct small capsid formation, which involves alteration of an internal scaffold. While many phages use internal scaffolding proteins, the involvement of an internal scaffold in capsid size redirection is novel.
Advisors/Committee Members: Gail Christie.
Subjects/Keywords: Medicine and Health Sciences
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APA (6th Edition):
Damle, P. (2011). Helper phage capsid size redirection by staphylococcal pathogenicity island SaPI1 involves internal scaffolding proteins. (Doctoral Dissertation). Virginia Commonwealth University. Retrieved from https://scholarscompass.vcu.edu/etd/255
Chicago Manual of Style (16th Edition):
Damle, Priyadarshan. “Helper phage capsid size redirection by staphylococcal pathogenicity island SaPI1 involves internal scaffolding proteins.” 2011. Doctoral Dissertation, Virginia Commonwealth University. Accessed June 23, 2018.
https://scholarscompass.vcu.edu/etd/255.
MLA Handbook (7th Edition):
Damle, Priyadarshan. “Helper phage capsid size redirection by staphylococcal pathogenicity island SaPI1 involves internal scaffolding proteins.” 2011. Web. 23 Jun 2018.
Vancouver:
Damle P. Helper phage capsid size redirection by staphylococcal pathogenicity island SaPI1 involves internal scaffolding proteins. [Internet] [Doctoral dissertation]. Virginia Commonwealth University; 2011. [cited 2018 Jun 23].
Available from: https://scholarscompass.vcu.edu/etd/255.
Council of Science Editors:
Damle P. Helper phage capsid size redirection by staphylococcal pathogenicity island SaPI1 involves internal scaffolding proteins. [Doctoral Dissertation]. Virginia Commonwealth University; 2011. Available from: https://scholarscompass.vcu.edu/etd/255
University of Wisconsin – Milwaukee
21.
Chawla, Ayesha.
Regulation of Anti-Angiogenic Pedf Through a TSP-1 - CD36 Pathway in Prostate Cancer.
Degree: MS, Biomedical Sciences, 2013, University of Wisconsin – Milwaukee
URL: https://dc.uwm.edu/etd/672
► Prostate cancer (PCa) is the most common type of cancer diagnosed in American men. Cancer progression is associated with increased angiogenesis, and thrombospondin-1 (TSP–1)…
(more)
▼ Prostate cancer (PCa) is the most common type of cancer diagnosed in American men. Cancer progression is associated with increased angiogenesis, and thrombospondin-1 (TSP–1) and pigment epithelium derived factor (PEDF), both potent anti-angiogenic molecules, are downregulated in PCa cells. TSP-1 exerts its activity through binding to several cell surface receptors such as CD36. Both TSP-1 and PEDF are multi-functional proteins and have been linked to lipid metabolism in other cell types. Moreover, TSP-1 - PEDF regulatory loops have been identified in some cell types. PEDF has been shown to inhibit PCa growth through its effects on angiogenesis and directly on the PCa cells; however, how PEDF expression levels are regulated in prostate cells is currently unknown. Here, we hypothesized that TSP-1 may regulate PEDF expression and lipid metabolism in PCa cells.
I collected and examined PEDF levels in both cell lysate and serum-free conditioned media samples from TSP-1 and anti-CD36 antibody treated prostate cells and examined PEDF levels. Both TSP-1 and anti-CD36 treatment increased PEDF expression in normal prostate epithelial cells, RWPE-1, and in PCa cells, PC-3 and LNCaP. The expression of candidate TSP-1 - CD36 signaling mediators, fyn, p38 MAPK and JNK, were also examined in treated samples. I found that TSP-1 treatment elevated expression of fyn, p38 and JNK in PC-3 and DU145 cells. In contrast, blocking the CD36 receptor diminished the expression of each signaling mediator.
My results are the first to show that a regulatory loop exists between TSP-1 and PEDF in prostate cells. The observation that treatment with anti-CD36 antibody also increased PEDF suggests that TSP-1 regulation of PEDF may be mediated through the CD36 receptor. These observations suggest that one mechanism of PEDF down-regulation in PCa cells may be due to loss of TSP-1 expression. Moreover, this pathway could be exploited for novel therapeutic interventions.
Advisors/Committee Members: Jennifer A. Doll.
Subjects/Keywords: Medicine and Health Sciences
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APA ·
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APA (6th Edition):
Chawla, A. (2013). Regulation of Anti-Angiogenic Pedf Through a TSP-1 - CD36 Pathway in Prostate Cancer. (Thesis). University of Wisconsin – Milwaukee. Retrieved from https://dc.uwm.edu/etd/672
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Chawla, Ayesha. “Regulation of Anti-Angiogenic Pedf Through a TSP-1 - CD36 Pathway in Prostate Cancer.” 2013. Thesis, University of Wisconsin – Milwaukee. Accessed June 23, 2018.
https://dc.uwm.edu/etd/672.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Chawla, Ayesha. “Regulation of Anti-Angiogenic Pedf Through a TSP-1 - CD36 Pathway in Prostate Cancer.” 2013. Web. 23 Jun 2018.
Vancouver:
Chawla A. Regulation of Anti-Angiogenic Pedf Through a TSP-1 - CD36 Pathway in Prostate Cancer. [Internet] [Thesis]. University of Wisconsin – Milwaukee; 2013. [cited 2018 Jun 23].
Available from: https://dc.uwm.edu/etd/672.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Chawla A. Regulation of Anti-Angiogenic Pedf Through a TSP-1 - CD36 Pathway in Prostate Cancer. [Thesis]. University of Wisconsin – Milwaukee; 2013. Available from: https://dc.uwm.edu/etd/672
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
University of Wisconsin – Milwaukee
22.
Sheerin, Shabistan.
Innovation and Commercialization Practices - A Qualitative Analysis of Novascan LLC.
Degree: PhD, Health Sciences, 2013, University of Wisconsin – Milwaukee
URL: https://dc.uwm.edu/etd/760
► The processes for commercialization of medical devices in healthcare are complex and varied, and it has been difficult to define the ingredients of success.…
(more)
▼ The processes for commercialization of medical devices in healthcare are complex and varied, and it has been difficult to define the ingredients of success. There exists a need to better understand evidence based best practices as there is lack of documented evidence based on best practices for commercialization of medical devices by startups. Commercialization of innovative medical devices in healthcare is in constant demand and the reasons are many fold. Most of the research based startups act as agents of economic development and therefore they need to function more efficiently and effectively. There exists a constant demand from end users to improve medical techniques, results patient experiences and cost effectiveness. However, a large number of strong and commercially viable innovations in healthcare fail to achieve commercialization.
The purpose of this paper is to build a theory. The study examines qualitatively commercialization practices of case study NovaScan LLC, a breast cancer detection device company. Through this single case study, various performances indicators of the commercialization steps followed by the company are identified and findings are presented in the form of theoretical propositions. Extensive literature review and analysis helped in better understanding of the process of commercialization from both healthcare and non-healthcare perspective. Data gathering, which focused on the above mentioned aim was carried on for nearly over four years, initially as an outsider participant and then in the latter part of study, as an insider participant. The data consisted of observations, informal conversations both via telephone and in-person, using an unstructured interview protocol, field notes, company archives and other historical data. Data collection participants were those institutional officials who were responsible both directly and indirectly for the innovation and commercialization activities at NovaScan LLC. All observations, conversations, field notes, documents and other records have been documented. The analysis for this study involves continuous back and forth linking of theory presented by literature findings and data obtained at NovaScan LLC. For the purpose of data analysis, the data is not coded sentence by sentence; rather it's focused on theme identification based on underlying meaning.
The results verify the impressions of many practitioners in the field of innovation and commercialization of medical devices in healthcare. The findings are presented in the form of seven propositions and also propose a framework of commercialization. These findings are recommended to be tested in future. Various activities related to commercialization process do not happen in isolation with product development in a startup firm like NovaScan LLC. Commercialization strategies are an integral part of development work and are well-aligned with the development process and all stages of development process overlap with each other.
Advisors/Committee Members: Timothy B. Patrick.
Subjects/Keywords: Business; Medicine and Health Sciences
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APA ·
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MLA ·
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Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Sheerin, S. (2013). Innovation and Commercialization Practices - A Qualitative Analysis of Novascan LLC. (Doctoral Dissertation). University of Wisconsin – Milwaukee. Retrieved from https://dc.uwm.edu/etd/760
Chicago Manual of Style (16th Edition):
Sheerin, Shabistan. “Innovation and Commercialization Practices - A Qualitative Analysis of Novascan LLC.” 2013. Doctoral Dissertation, University of Wisconsin – Milwaukee. Accessed June 23, 2018.
https://dc.uwm.edu/etd/760.
MLA Handbook (7th Edition):
Sheerin, Shabistan. “Innovation and Commercialization Practices - A Qualitative Analysis of Novascan LLC.” 2013. Web. 23 Jun 2018.
Vancouver:
Sheerin S. Innovation and Commercialization Practices - A Qualitative Analysis of Novascan LLC. [Internet] [Doctoral dissertation]. University of Wisconsin – Milwaukee; 2013. [cited 2018 Jun 23].
Available from: https://dc.uwm.edu/etd/760.
Council of Science Editors:
Sheerin S. Innovation and Commercialization Practices - A Qualitative Analysis of Novascan LLC. [Doctoral Dissertation]. University of Wisconsin – Milwaukee; 2013. Available from: https://dc.uwm.edu/etd/760
Virginia Commonwealth University
23.
Bertsche, Joseph.
Histone Deacetylase Inhibitors and Innate Immunity in Septic Shock.
Degree: MS, Microbiology & Immunology, 2009, Virginia Commonwealth University
URL: https://scholarscompass.vcu.edu/etd/2011
► Innate immunity depends on pattern recognition receptors, which recognize pathogen associated molecular patterns (PAMPS), such as Toll-like receptor 4 (TLR4), which detects the gram-negative bacterial…
(more)
▼ Innate immunity depends on pattern recognition receptors, which recognize pathogen associated molecular patterns (PAMPS), such as Toll-like receptor 4 (TLR4), which detects the gram-negative bacterial toxin, lipopolysaccharide. Engagement of TLR4 by LPS sets off a cascade ending in the activation of pro-inflammatory cytokines and interferon-β (IFN-β) which alerts the host to the infection. However, these responses can be mal-adaptive, especially in the context of bacterial sepsis, where a "cytokine storm" results in death of the host. Pharmacological modulation of these responses may therefore be a promising treatment modality. Inhibition of classic pro-inflammatory cytokines such as IL-1β and TNF-α has been the (largely unfruitful) focus of much research. However it has recently emerged that mice with defects in type I IFN signaling are also substantially resistant to challenge with endotoxin. We therefore wish to investigate pharmacological inhibition of IFN signaling as a potential means to control sepsis. We analyzed the effects of Trichostatin A (TSA) and Suberoylanilide hydroxamic acid (SAHA) (both broad spectrum HDAC inhibitors) and ST-2-92 (HDAC6 specific inhibitor) on IFN regulation and endotoxic shock. We created an in vivo mouse model for this treatment with TSA and SAHA (which are well tolerated in mouse and human) to look for possible alteration in the survival rate following endotoxin challenge. We as well as others found that treatment with SAHA (50mg/kg) significantly improves survival rate. We also characterized in-vitro modulation of IFN responses through SAHA by mouse DNA microarray. We noticed a decreased expression of many innate immune regulated genes in the SAHA and LPS treated condition compared to the LPS treatment alone. Additionally we observed a decrease in protein levels of IFN-β IL-1β, IL-6, IL-12p40, RANTES and TNF-α in cell culture supernatants treated with SAHA or ST-2-92 and LPS compared to LPS only treatment.
These results show the ability of broad spectrum HDACi through SAHA to increase mouse survival following LPS challenge as well as modulate the induction of innate immune responsive genes in vitro. Furthermore we have shown that HDAC specific inhibition through ST-2-92 can decrease pro-inflammatory transcript as well as protein levels.
Advisors/Committee Members: Paul Fawcett.
Subjects/Keywords: Medicine and Health Sciences
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APA (6th Edition):
Bertsche, J. (2009). Histone Deacetylase Inhibitors and Innate Immunity in Septic Shock. (Thesis). Virginia Commonwealth University. Retrieved from https://scholarscompass.vcu.edu/etd/2011
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Bertsche, Joseph. “Histone Deacetylase Inhibitors and Innate Immunity in Septic Shock.” 2009. Thesis, Virginia Commonwealth University. Accessed June 23, 2018.
https://scholarscompass.vcu.edu/etd/2011.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Bertsche, Joseph. “Histone Deacetylase Inhibitors and Innate Immunity in Septic Shock.” 2009. Web. 23 Jun 2018.
Vancouver:
Bertsche J. Histone Deacetylase Inhibitors and Innate Immunity in Septic Shock. [Internet] [Thesis]. Virginia Commonwealth University; 2009. [cited 2018 Jun 23].
Available from: https://scholarscompass.vcu.edu/etd/2011.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Bertsche J. Histone Deacetylase Inhibitors and Innate Immunity in Septic Shock. [Thesis]. Virginia Commonwealth University; 2009. Available from: https://scholarscompass.vcu.edu/etd/2011
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Virginia Commonwealth University
24.
Farah, Christine.
The Fluoride Recharging Capability of Orthodontic Materials: an in-vitro study.
Degree: MS, Dentistry, 2012, Virginia Commonwealth University
URL: https://scholarscompass.vcu.edu/etd/2682
► Enamel demineralization in the form of white spot lesions (WSLs) around fixed orthodontic appliances is a persistent problem in patients with poor oral hygiene.These lesions…
(more)
▼ Enamel demineralization in the form of white spot lesions (WSLs) around fixed orthodontic appliances is a persistent problem in patients with poor oral hygiene.These lesions can form rapidly within 4 weeks of bracket placement.The purpose of this in-vitro study was to investigate the fluoride recharging capability ofa commercially available orthodontic primer used to minimize the development of WSLs in patients.
The three groups tested were: OpalSeal (n=20, Ultradent, South Jordan, UT), ProSeal (n=20, Reliance, Itasca, IL) and Transbond XT (control, n=10, 3M Unitek, Monrovia, CA). The samples(5mmin diameter x 1mm in thickness) weresuspended individually in vials filled with 10mL of deionized water usinga fishing line. The baseline fluoride ion release from all of the samples was measured after two weeks of changing the solution every other day. The samples were then randomly divided into two groups, toothbrush or gel. The samples in the toothbrush group were brushed for one minute every day for 7d, with fluoride containing toothpaste (Colgate-Palmolive Company, New York, NY) and placed in a new solution after each brushing. After 7d of brushing the fluoride ion release was measured. The samples in the gel group were immersed in 10mL of acidulated phosphoric fluoride gel (APF) for one minute, following manufacturer’s instructions, and then placed in a new vial with 10mL of deionized water. At the end of 24hrs fluoride ion release measurementswere made and the samples were placed individually in a new solution. The solution was changed weekly in the gel group over six weeks to simulate the typical length of time between two orthodontic appointments. A final fluoride ion release measurement was taken of all the discs in the gel group 6 weeks after the fluoride gel treatment.
The results of repeated-measures analysis indicated that there were no significant differences between the groups at baseline and after 7d of toothbrushing time points. Opal Seal exhibited a significant increase in fluoride uptake (1.0ppm) after 24hrs of fluoride gel exposure but these levels gradually decreasedover 6 weeks (0.04ppm). Pro Seal and Transbond showed no significant fluoride release after the gel or toothpaste applications. The fluoride-containing primer, Opal Seal, had the ability to be recharged with fluoride ions from APF gel. However, the amount of fluoride released from recharged discs decreased gradually over a 6 weeks of time.
Advisors/Committee Members: Eser Tufekci.
Subjects/Keywords: Dentistry; Medicine and Health Sciences
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APA (6th Edition):
Farah, C. (2012). The Fluoride Recharging Capability of Orthodontic Materials: an in-vitro study. (Thesis). Virginia Commonwealth University. Retrieved from https://scholarscompass.vcu.edu/etd/2682
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Farah, Christine. “The Fluoride Recharging Capability of Orthodontic Materials: an in-vitro study.” 2012. Thesis, Virginia Commonwealth University. Accessed June 23, 2018.
https://scholarscompass.vcu.edu/etd/2682.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Farah, Christine. “The Fluoride Recharging Capability of Orthodontic Materials: an in-vitro study.” 2012. Web. 23 Jun 2018.
Vancouver:
Farah C. The Fluoride Recharging Capability of Orthodontic Materials: an in-vitro study. [Internet] [Thesis]. Virginia Commonwealth University; 2012. [cited 2018 Jun 23].
Available from: https://scholarscompass.vcu.edu/etd/2682.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Farah C. The Fluoride Recharging Capability of Orthodontic Materials: an in-vitro study. [Thesis]. Virginia Commonwealth University; 2012. Available from: https://scholarscompass.vcu.edu/etd/2682
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Virginia Commonwealth University
25.
Balinang, Joyce.
The Regulation of Mitochondrial DNMT1 During Oxidative Stress.
Degree: MS, Microbiology & Immunology, 2012, Virginia Commonwealth University
URL: https://scholarscompass.vcu.edu/etd/2826
► Epigenetics is the study of heritable gene expression due to alterations in the DNA structure other than the underlying DNA sequence. DNA methylation is one…
(more)
▼ Epigenetics is the study of heritable gene expression due to alterations in the DNA structure other than the underlying DNA sequence. DNA methylation is one of the three types of epigenetic modifications found in the eukaryotic system. It involves the incorporation of a methyl group at the 5-position of cytosine residues in the DNA. DNA methylation is associated with several notorious disorders and diseases including Fragile X Syndrome, neurodegenerative disease (Parkinson’s, Alzhiemer, etc), diabetes and cancer. Cytosine methylation of mitochondrial DNA (mtDNA) was first demonstrated several decades ago but the mechanism of generating cytosine modification and its functional importance remain elusive. Our laboratory recently demonstrated that the enzyme involved in cytosine modification of mtDNA is a novel mitochondrial isoform of DNA Methyltransferase 1, mtDNMT1. This protein is encoded in the nucleus and targeted to the mitochondria via a N-terminal targeting sequence. Bioinformatic analysis of the DNMT1 coding sequence showed a consensus NRF1 binding site that coincidently overlaps a p53 binding site within the promoter region, previously shown by this group to repress DNMT1 expression. Previous studies in the Taylor laboratory showed that mtDNMT protein expression was regulated by the transcription factor NRF1 as well as its coactivator PGC1α. PGC1α and NRF1 stimulate a large body of genes that are involved in mitochondrial biogenesis and cellular respiration in response to environmental stress. Considering the previous findings in our laboratory regarding mtDNMT1 regulation and the importance of PGC1α and NRF1 in oxidative homeostasis, we asked whether there is a mitochondrial epigenetic component in the cell’s response to cellular stress and whether up-regulation of mtDNMT1 might be part of the general response to this stress. To investigate the relationship between mtDNA methylation and oxidative homeostasis we examined the regulation of mtDNMT1 by transcription factors that respond to oxidative stress. Conditions that induced oxidative stress were applied to HCT 116 and SH-SY5Y cell lines and the protein expression of DNMT1 was observed. Ethanol and hypoxia- induced oxidative stress were observed to increase to protein level of mtDNMT1 while total DNMT1 level either remained constant or decreased. The protein level of PGC1α and NRF1 remained low in HCT 116 cells exposed to hypoxic stress, despite elevated mtDNMT1 protein level. ChIP analysis of HCT 116 cells exposed to hypoxic stress demonstrated that NRF1 and PGC1α are not regulating the transcription of DNMT1i in the mitochondria. However, we observed that p53 dissociated from the DNMT1 promoter upon hypoxic stress, indicating that the up-regulation of mtDNMT1 is through the relief of p53 suppression. The findings of this investigation proved that mtDNMT1 is receptive to oxidative stress through the regulation by p53 and suggested that mitochondrial epigenetics may be playing an integral role in the cellular stress response toward hypoxia.
Advisors/Committee Members: Shirley Taylor.
Subjects/Keywords: Medicine and Health Sciences
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APA (6th Edition):
Balinang, J. (2012). The Regulation of Mitochondrial DNMT1 During Oxidative Stress. (Thesis). Virginia Commonwealth University. Retrieved from https://scholarscompass.vcu.edu/etd/2826
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Balinang, Joyce. “The Regulation of Mitochondrial DNMT1 During Oxidative Stress.” 2012. Thesis, Virginia Commonwealth University. Accessed June 23, 2018.
https://scholarscompass.vcu.edu/etd/2826.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Balinang, Joyce. “The Regulation of Mitochondrial DNMT1 During Oxidative Stress.” 2012. Web. 23 Jun 2018.
Vancouver:
Balinang J. The Regulation of Mitochondrial DNMT1 During Oxidative Stress. [Internet] [Thesis]. Virginia Commonwealth University; 2012. [cited 2018 Jun 23].
Available from: https://scholarscompass.vcu.edu/etd/2826.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Balinang J. The Regulation of Mitochondrial DNMT1 During Oxidative Stress. [Thesis]. Virginia Commonwealth University; 2012. Available from: https://scholarscompass.vcu.edu/etd/2826
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Grand Valley State University
26.
Quinn, Josh.
Effects of Chronic Atrazine Exposure on Male Reproductive Function in Sprague Dawley Rats.
Degree: 2018, Grand Valley State University
URL: https://scholarworks.gvsu.edu/theses/872
► Estrogens, in addition to testosterone, are much more physiologically relevant to normal sperm production in the testis and overall male fertility than previously thought. Current…
(more)
▼ Estrogens, in addition to testosterone, are much more physiologically relevant to normal sperm production in the testis and overall male fertility than previously thought. Current use herbicides, such as atrazine, are known endocrine disruptors and have been suggested to disrupt male reproductive health. The purpose of this study was to investigate the effect of chronic atrazine exposure on male reproductive function in Sprague Dawley rats. Three groups of rats were treated until 7 months of age. The first group was the control vehicle and given only water. The second group was given a low dose of atrazine (0.1 mg/kg) and the third group was given a high dose of atrazine (10 mg/kg) via oral gavage to mimic the most common exposure to atrazine in humans. At 7 months of age, animals were euthanized and reproductive tissues were collected for analysis. Concentrations of testosterone were not significantly different between groups, but levels of serum FSH and estradiol were significantly increased in the high dose group relative to the control. Sperm/gram of the testis was significantly lower in the low dose group and daily sperm production was significantly lower in the high dose group. Collectively, these results support the hypothesis that sperm production and overall male reproductive health is significantly disrupted by chronic exposure to atrazine, and the relationship between estradiol and male fertility is sensitive to environmental contaminants.
Subjects/Keywords: Medicine and Health Sciences
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APA ·
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CSE |
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APA (6th Edition):
Quinn, J. (2018). Effects of Chronic Atrazine Exposure on Male Reproductive Function in Sprague Dawley Rats. (Thesis). Grand Valley State University. Retrieved from https://scholarworks.gvsu.edu/theses/872
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Quinn, Josh. “Effects of Chronic Atrazine Exposure on Male Reproductive Function in Sprague Dawley Rats.” 2018. Thesis, Grand Valley State University. Accessed June 23, 2018.
https://scholarworks.gvsu.edu/theses/872.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Quinn, Josh. “Effects of Chronic Atrazine Exposure on Male Reproductive Function in Sprague Dawley Rats.” 2018. Web. 23 Jun 2018.
Vancouver:
Quinn J. Effects of Chronic Atrazine Exposure on Male Reproductive Function in Sprague Dawley Rats. [Internet] [Thesis]. Grand Valley State University; 2018. [cited 2018 Jun 23].
Available from: https://scholarworks.gvsu.edu/theses/872.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Quinn J. Effects of Chronic Atrazine Exposure on Male Reproductive Function in Sprague Dawley Rats. [Thesis]. Grand Valley State University; 2018. Available from: https://scholarworks.gvsu.edu/theses/872
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
27.
Nyznyk, Julia.
Decreasing Medication Errors Through the Reduction of Avoidable Interruptions.
Degree: MSN, 2014, University of San Francisco
URL: https://repository.usfca.edu/capstone/66
► In recent years, the media has been rife with stories of medical mistakes in hospitals or other health care settings that lead to patient…
(more)
▼ In recent years, the media has been rife with stories of medical mistakes in hospitals or other health care settings that lead to patient injury or death. While most of the media attention rightfully focuses on the horrific psychological and emotional toll such injuries and deaths impose upon patients, their loved ones, and the healthcare providers involved, there is also an enormous economic cost associated with the results of such mistakes that, if not resolved, will continue to strain an already heavily burdened system. Progressive step down units are fast paced and highly demanding. This type of unit is placed between the intensive care units and medical/surgical units. The staff is in constant motion however there has been an increase in medication errors that has lead to a halted workflow and ultimately a less efficient unit. The cause of this increase is multifactorial however the focus of this paper will be on the interruptions occurring during medication passes, interruptions that can have severely detrimental effects on patients and their safety.
Subjects/Keywords: Medicine and Health Sciences
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APA ·
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to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Nyznyk, J. (2014). Decreasing Medication Errors Through the Reduction of Avoidable Interruptions. (Thesis). University of San Francisco. Retrieved from https://repository.usfca.edu/capstone/66
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Nyznyk, Julia. “Decreasing Medication Errors Through the Reduction of Avoidable Interruptions.” 2014. Thesis, University of San Francisco. Accessed June 23, 2018.
https://repository.usfca.edu/capstone/66.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Nyznyk, Julia. “Decreasing Medication Errors Through the Reduction of Avoidable Interruptions.” 2014. Web. 23 Jun 2018.
Vancouver:
Nyznyk J. Decreasing Medication Errors Through the Reduction of Avoidable Interruptions. [Internet] [Thesis]. University of San Francisco; 2014. [cited 2018 Jun 23].
Available from: https://repository.usfca.edu/capstone/66.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Nyznyk J. Decreasing Medication Errors Through the Reduction of Avoidable Interruptions. [Thesis]. University of San Francisco; 2014. Available from: https://repository.usfca.edu/capstone/66
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
28.
Singh, Gagandeep.
Reduce the Rooming Time.
Degree: MSN, 2016, University of San Francisco
URL: https://repository.usfca.edu/capstone/285
► The aim of the project Reducing the Rooming Time by the Medical Assistant is to reduce the rooming time from thirteen minutes to ten…
(more)
▼ The aim of the project Reducing the Rooming Time by the Medical Assistant is to reduce the rooming time from thirteen minutes to ten minutes. In the Department of Adult and Family Medicine, there are about eighty physicians who are supported by eighty medical assistants. The appointment length is twenty minutes. The rooming procedures take about thirteen minutes to complete which leaves only seven minutes for physicians to see their patients. This has led to patients, physicians, and staff unsatisfied.
A team was created which include physicians, medical assistants, and receptionists. Data was obtained by the system’s analyst that looked at the time spent by each MA in the patient’s chart to document the rooming procedure. The team utilized performance improvement tools such as SWOT analysis, fishbone diagram, and a process map to identify areas of opportunities. The test of change was identified by the team that will aid in reducing the rooming time.
The first test of change was delayed due to data issues and staff adherence. Few changes were made to test the change again. Rooming project has the potential to improve quality of care and patient satisfaction. It also has the potential to improve staff and physicians morale.
Subjects/Keywords: Medicine and Health Sciences
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Export
to Zotero / EndNote / Reference
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APA (6th Edition):
Singh, G. (2016). Reduce the Rooming Time. (Thesis). University of San Francisco. Retrieved from https://repository.usfca.edu/capstone/285
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Singh, Gagandeep. “Reduce the Rooming Time.” 2016. Thesis, University of San Francisco. Accessed June 23, 2018.
https://repository.usfca.edu/capstone/285.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Singh, Gagandeep. “Reduce the Rooming Time.” 2016. Web. 23 Jun 2018.
Vancouver:
Singh G. Reduce the Rooming Time. [Internet] [Thesis]. University of San Francisco; 2016. [cited 2018 Jun 23].
Available from: https://repository.usfca.edu/capstone/285.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Singh G. Reduce the Rooming Time. [Thesis]. University of San Francisco; 2016. Available from: https://repository.usfca.edu/capstone/285
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
29.
Balzaretti, Carlee Y.S.
Development of a Toolkit to Enhance Nursing Education of Veteran Health Concerns.
Degree: DNP, Nursing, 2016, University of San Francisco
URL: https://repository.usfca.edu/dnp/88
► <h1>Abstract</h1> U.S. Veterans are a vulnerable patient population, more prone to health disparities as compared to the general population. There are 21.8 million Veterans currently…
(more)
▼ <h1>Abstract</h1>
U.S. Veterans are a vulnerable patient population, more prone to
health disparities as compared to the general population. There are 21.8 million Veterans currently in the United States, with the majority receiving their healthcare outside of the Veteran
Health Administration. Veterans form a culture that is steeped in military tradition and it is essential that healthcare providers have knowledge regarding this vulnerable population to provide care that is culturally appropriate. In response to the Veteran healthcare crisis, the Department of Veteran Affairs initiated the Veteran Affairs Nursing Academy Partnership (VANAP), a joint venture between VHA medical centers and schools of nursing, with a focus on caring for Veteran patients. One outcome of these partnerships was the evidence-based development of ten competencies designed to provide nursing students with the minimal preparation necessary in caring for Veterans. Given the majority of Veterans seek healthcare outside of the VHA system, these competencies should not be limited to students in a VANAP program. The University of San Francisco, being part of the VANAP initiative, has a great opportunity to bring these competencies to all of its students. The focus of this project was to create an evidence-based toolkit for nurse educators, containing ten educational modules based on the ten competencies. The modules were evaluated by Veteran and education
subject matter experts for appropriateness of content, being able to meet the metrics of knowledge acquisition, and ease of use. They will be available online to allow for easy accessibility.
Key Words: Veterans, healthcare, PTSD, homelessness, TBI, end of life, suicide, amputation, military, exposures, MST, substance use, disparities, toolkit, effectiveness, cultural competence, cultural sensitivity, cultural awareness, nursing students, nursing, VANAP.
Advisors/Committee Members: Juli Maxworthy, Timothy Godfrey, Neftali Cabezudo.
Subjects/Keywords: Medicine and Health Sciences; Nursing
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APA ·
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APA (6th Edition):
Balzaretti, C. Y. S. (2016). Development of a Toolkit to Enhance Nursing Education of Veteran Health Concerns. (Thesis). University of San Francisco. Retrieved from https://repository.usfca.edu/dnp/88
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Balzaretti, Carlee Y S. “Development of a Toolkit to Enhance Nursing Education of Veteran Health Concerns.” 2016. Thesis, University of San Francisco. Accessed June 23, 2018.
https://repository.usfca.edu/dnp/88.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Balzaretti, Carlee Y S. “Development of a Toolkit to Enhance Nursing Education of Veteran Health Concerns.” 2016. Web. 23 Jun 2018.
Vancouver:
Balzaretti CYS. Development of a Toolkit to Enhance Nursing Education of Veteran Health Concerns. [Internet] [Thesis]. University of San Francisco; 2016. [cited 2018 Jun 23].
Available from: https://repository.usfca.edu/dnp/88.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Balzaretti CYS. Development of a Toolkit to Enhance Nursing Education of Veteran Health Concerns. [Thesis]. University of San Francisco; 2016. Available from: https://repository.usfca.edu/dnp/88
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
30.
Saini, Ritu.
Understanding and Prevention of Catheter Associated Urinary Tract Infections (CAUTIs).
Degree: MSN, 2018, University of San Francisco
URL: https://repository.usfca.edu/capstone/740
► Abstract The project goal is to reduce and eliminate CAUTI occurrences on the med-surgical unit by increasing staff knowledge on CAUTIs and applying the…
(more)
▼ Abstract
The project goal is to reduce and eliminate CAUTI occurrences on the med-surgical unit by increasing staff knowledge on CAUTIs and applying the ANA CAUTI prevention tool to improve patient outcomes. The project objectives are (1) enhanced staff knowledge about CAUTIs, (2) staff adherence to the implemented the ANA CAUTI prevention tool, and (3) maintained CAUTI rate below benchmark. The CNL role in the project is to function as an educator and outcomes manager, which serves as the foundation for the project. The department consists of a 34-bed med-surgical unit with the adult patient population mix of oncology, surgical, and cardiac patients. The unit currently utilizes the ANA CAUTI Prevention Tool; however, the rate of CAUTIs continues to increase. The current CAUTI rate for the medical-surgical unit is 1.52 for year 2017 which is above the national benchmark through National Healthcare Safety Network (NHSN). The project goal is to decrease or eliminate CAUTI rate on the unit by 54% for 2018 to bring the rate below the benchmark. The risk factors from root-case, stakeholders and SWOT analysis are categorized into four categories such as human factors, environmental factors, administrative factors and material factors. The project status is in progress. The evaluation plan includes data collection, data analysis, and data comparison. The CAUTI incidences will be measured and collected by infection control daily audit tool explicitly created to CAUTIs. The infection control nurse will be responsible for obtaining this information. Data analysis includes Catheter days, indications, and compliance with ANA tool will be measured through chart review by CAUTI task force and infection control daily audit. The risk analysis department will compare the data to evaluate the quarterly trend and address barriers. The data will help evaluate the reduction in CAUTI rate and staff compliance with ANA CAUTI prevention tool. The project will be sustained through (1) stakeholders’ support, (2) CAUTI task force involvement, (3) by using ANA CAUTI prevention tool appropriately, (4) providing continuous education support, (5) address barriers and challenges and (6) staff recognition. The frontline nursing staff can make the most difference through sound knowledge of CAUTIs, current practice guidelines, and ANA prevention tool.
Subjects/Keywords: Medicine and Health Sciences
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APA (6th Edition):
Saini, R. (2018). Understanding and Prevention of Catheter Associated Urinary Tract Infections (CAUTIs). (Thesis). University of San Francisco. Retrieved from https://repository.usfca.edu/capstone/740
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Saini, Ritu. “Understanding and Prevention of Catheter Associated Urinary Tract Infections (CAUTIs).” 2018. Thesis, University of San Francisco. Accessed June 23, 2018.
https://repository.usfca.edu/capstone/740.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Saini, Ritu. “Understanding and Prevention of Catheter Associated Urinary Tract Infections (CAUTIs).” 2018. Web. 23 Jun 2018.
Vancouver:
Saini R. Understanding and Prevention of Catheter Associated Urinary Tract Infections (CAUTIs). [Internet] [Thesis]. University of San Francisco; 2018. [cited 2018 Jun 23].
Available from: https://repository.usfca.edu/capstone/740.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Saini R. Understanding and Prevention of Catheter Associated Urinary Tract Infections (CAUTIs). [Thesis]. University of San Francisco; 2018. Available from: https://repository.usfca.edu/capstone/740
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
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Open Access Theses and Dissertations