subject:(Matrix Metalloproteinases)

1. Fountoulakis, Nikitas. Εκτίμηση των επιπέδων των μεταλλοπρωτεϊνασών και των αναστολέων τους στο υδατοειδές υγρό ασθενών με πρωτοπαθές γλαύκωμα ανοικτής γωνίας, σύνδρομο ψευδοαποφολίδωσης και ψευδοαποφολιδωτικό γλαύκωμα: ο ρόλος του νέου αναστολέα 4 των μεταλλοπρωτεϊνασών στη πρωτεολυτική ισορροπία και η συσχέτισή της με κλινικοεργαστηριακούς δείκτες.

Degree: 2014, Democritus University of Thrace (DUTH); Δημοκρίτειο Πανεπιστήμιο Θράκης (ΔΠΘ)

URL: http://hdl.handle.net/10442/hedi/35000

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The purpose of this study is to quantify the levels of tissue inhibitor of metalloproteinase 4 (TIMP4) and its ratios with free and active metalloproteinases… (more)

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The purpose of this study is to quantify the levels of tissue inhibitor of metalloproteinase 4 (TIMP4) and its ratios with free and active metalloproteinases (MMP) in the aqueous humor of patients with primary open angle glaucoma (POAG), pseudoexfoliation syndrome (PXS) and pseudoexfoliative glaucoma (PXG) and to evaluate a possible imbalance between MMPs and TIMPs in these samples. It is also attempted to correlate the levels of free and active MMP and TIMP in the aqueous humor of the same patients with different clinical parameters influencing or influenced by disease progression.Methods: Free MMP2, MMP3, MMP9, TIMP1, TIMP2, TIMP4 concentrations andactive levels of MMP2 and MMP3 were determined with immunoassay ELISA andactivity assay kits in 168 aqueous samples. Age, intraocular pressure, c outflow coefficient, medication and c/d ratio were registered and correlated with MMP and TIMP levels.Results: TIMP4 was elevated in glaucoma patients. POAG, PXS and PXG samples demonstrated higher MMP2, TIMP1 and TIMP2 concentrations.Samples from the PXS and PXG groups had a lower total/active MMP2 ratio. Stoichiometric analysis showed an overbalance of TIMPs, especially TIMP4, over MMPs in both POAG &PXG groups. Furthermore POAG patiens presented a positive correlation between TIMP1-2 levels and intraocular pressure raise and a negative correlation between TIMP4 and active MMP2 levels and c outflow coefficient. In PXS patients, intraocular pressure raise was correlated with diminished levels of TIMP1and increased levels of active MMP3. Age and c outflow coefficient were also positively correlated with increased levels of total and active MMP3. In PXG patients a negative correlation was found between MMP3concentration and intraocular pressure and between TIMP4 levels and outflow facility. In glaucoma groups, increased medication use is accompanied with rising MMP levels.Conclusion: TIMP4 elevation is a novel finding in glaucomatous eyes. Adisregulation of extracellular matrix homeostasis is also suggested in PXS but mainly in POAG and PXG. Finally, patients with the same pathologic condition presented significant differences in their aqueous catabolic status, depending on variable clinical features. This could suggest an additional explanation on disease variability and progression.

Ο σκοπός της παρούσας μελέτης είναι ο ποσοτικός προσδιορισμός ενός νέου αναστολέα των μεταλλοπρωτεινασών, τον ΤΙΜΡ4, στο υδατοειδές υγρό ασθενών με πρωτοπαθές γλαύκωμα ανοικτής γωνίας, με σύνδρομο ψευδοαποφολίδωσης και με ψευδοαποφολιδωτικό γλαύκωμα και ο υπολογισμός των αναλογιών του με άλλες μεταλλοπρωτεινάσες(ΜΜΡ) του υδατοειδούς υγρού. Επίσης, στόχος της παρούσας έρευνας είναι η διερεύνηση ευρύτερα της ανισορροπίας των ελεύθερων και ενεργοποιημένων μεταλλοπρωτεινασων και των αναστολέων τους (ΤΙΜΡ) στο υδατοειδές υγρό των ίδιων ασθενών. Τέλος, ζητούμενο αποτελεί και η συσχέτιση των επιπέδων των παραπάνω ενζύμων και των αναστολέων τους με κλινικούς δεικτές ασθενών με την ίδια παθολογική οντότητα, που επηρεάζουν ή επηρεάζονται από την εξέλιξη…

Subjects/Keywords: Μεταλλοπρωτεϊνάσες; Matrix metalloproteinases

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Fountoulakis, N. (2014). Εκτίμηση των επιπέδων των μεταλλοπρωτεϊνασών και των αναστολέων τους στο υδατοειδές υγρό ασθενών με πρωτοπαθές γλαύκωμα ανοικτής γωνίας, σύνδρομο ψευδοαποφολίδωσης και ψευδοαποφολιδωτικό γλαύκωμα: ο ρόλος του νέου αναστολέα 4 των μεταλλοπρωτεϊνασών στη πρωτεολυτική ισορροπία και η συσχέτισή της με κλινικοεργαστηριακούς δείκτες. (Thesis). Democritus University of Thrace (DUTH); Δημοκρίτειο Πανεπιστήμιο Θράκης (ΔΠΘ). Retrieved from http://hdl.handle.net/10442/hedi/35000

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Fountoulakis, Nikitas. “Εκτίμηση των επιπέδων των μεταλλοπρωτεϊνασών και των αναστολέων τους στο υδατοειδές υγρό ασθενών με πρωτοπαθές γλαύκωμα ανοικτής γωνίας, σύνδρομο ψευδοαποφολίδωσης και ψευδοαποφολιδωτικό γλαύκωμα: ο ρόλος του νέου αναστολέα 4 των μεταλλοπρωτεϊνασών στη πρωτεολυτική ισορροπία και η συσχέτισή της με κλινικοεργαστηριακούς δείκτες.” 2014. Thesis, Democritus University of Thrace (DUTH); Δημοκρίτειο Πανεπιστήμιο Θράκης (ΔΠΘ). Accessed March 05, 2021. http://hdl.handle.net/10442/hedi/35000.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Fountoulakis, Nikitas. “Εκτίμηση των επιπέδων των μεταλλοπρωτεϊνασών και των αναστολέων τους στο υδατοειδές υγρό ασθενών με πρωτοπαθές γλαύκωμα ανοικτής γωνίας, σύνδρομο ψευδοαποφολίδωσης και ψευδοαποφολιδωτικό γλαύκωμα: ο ρόλος του νέου αναστολέα 4 των μεταλλοπρωτεϊνασών στη πρωτεολυτική ισορροπία και η συσχέτισή της με κλινικοεργαστηριακούς δείκτες.” 2014. Web. 05 Mar 2021.

Vancouver:

Fountoulakis N. Εκτίμηση των επιπέδων των μεταλλοπρωτεϊνασών και των αναστολέων τους στο υδατοειδές υγρό ασθενών με πρωτοπαθές γλαύκωμα ανοικτής γωνίας, σύνδρομο ψευδοαποφολίδωσης και ψευδοαποφολιδωτικό γλαύκωμα: ο ρόλος του νέου αναστολέα 4 των μεταλλοπρωτεϊνασών στη πρωτεολυτική ισορροπία και η συσχέτισή της με κλινικοεργαστηριακούς δείκτες. [Internet] [Thesis]. Democritus University of Thrace (DUTH); Δημοκρίτειο Πανεπιστήμιο Θράκης (ΔΠΘ); 2014. [cited 2021 Mar 05]. Available from: http://hdl.handle.net/10442/hedi/35000.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Fountoulakis N. Εκτίμηση των επιπέδων των μεταλλοπρωτεϊνασών και των αναστολέων τους στο υδατοειδές υγρό ασθενών με πρωτοπαθές γλαύκωμα ανοικτής γωνίας, σύνδρομο ψευδοαποφολίδωσης και ψευδοαποφολιδωτικό γλαύκωμα: ο ρόλος του νέου αναστολέα 4 των μεταλλοπρωτεϊνασών στη πρωτεολυτική ισορροπία και η συσχέτισή της με κλινικοεργαστηριακούς δείκτες. [Thesis]. Democritus University of Thrace (DUTH); Δημοκρίτειο Πανεπιστήμιο Θράκης (ΔΠΘ); 2014. Available from: http://hdl.handle.net/10442/hedi/35000

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Newcastle

2. Roy, Tammie K. Matrix metalloproteinase-2 in early embryo implantation in the mouse.

Degree: PhD, 2011, University of Newcastle

URL: http://hdl.handle.net/1959.13/927259

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Research Doctorate - Doctor of Philosophy (PhD)

In mammals the implantation of trophoblast cells into the uterus is highly invasive and requires the degradation of… (more)

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Research Doctorate - Doctor of Philosophy (PhD)

In mammals the implantation of trophoblast cells into the uterus is highly invasive and requires the degradation of the extracellular matrix (ECM) of the uterine wall. It was first hypothesised more than 70 years ago that the invasiveness of the trophoblast cells was due to proteolytic activity. Matrix metalloproteinases (MMPs) are a family of zinc and calcium dependent endopeptidases that in combination are capable of degrading virtually all of the components of the ECM, both interstitial matrix and basement membrane [1, 2]. Research into implantation in rodent and ovine models has implicated numerous members of the MMP family (MMP-2, -3, -7, -9, -11, -13 and MT1-MMP) in the control of early events of embryo implantation [3-9]. The majority of these studies agree that the most important of the MMPs in implantation are MMP-2 and MMP-9. The process of embryo attachment and invasion is one of the most biologically complex processes studied in reproductive biology. While evidence exists for the presence of MMPs during embryo implantation, knowledge is limited on the exact expression patterns and regulatory pathways that control these enzymes and allow for the invasion of trophoblast cells in the maternal endometrium. Previous research, undertaken by this research group, has looked at the expression of MMP-2 and MMP-9 during embryo implantation in the mouse using a new model of mating that allows for a more accurate timing of implantation events. The results from these experiments showed that there is a significant increase in the presence of MMP-2 in the luminal flushes around 97 – 98 hours post-insemination (day 4 of pregnancy). The aim of this project was to test the broad hypothesis that MMP-2 proteins are expressed and activated at the early stages of blastocyst implantation by cells of both endometrial and embryonic origin. Additionally, studies also tested the hypothesis that the expression of MMP-2 is specific to the events of embryo implantation and requires an interaction between the embryo and endometrial cells. An optimised mating protocol was used to facilitate more accurate identification of the early stages of implantation in order to be able to further define the expression patterns of MMP-2. Immunohistochemical labelling of MMP-2 proteins in formalin fixed sections of embryo implantation sites at specific times post-insemination localised MMP-2 expression to cells of both the blastocyst and endometrium. Specifically, antibody staining was identified in trophectoderm cells and inner cell mass cells of the embryo and in endometrial epithelial cells of the lumen and in the cells of the endometrium. While these experiments demonstrated MMP-2 protein localisation, they did not allow the assessment proteinase activity. The presence of gelatinase activity was identified through the application of in situ zymography to fresh frozen sections of tissue from implantation sites collected at specific times post-insemination. Results from these experiments confirmed…

Advisors/Committee Members: University of Newcastle. Faculty of Health, School of Biomedical Sciences and Pharmacy.

Subjects/Keywords: matrix metalloproteinases; embryo implantation; mice

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Roy, T. K. (2011). Matrix metalloproteinase-2 in early embryo implantation in the mouse. (Doctoral Dissertation). University of Newcastle. Retrieved from http://hdl.handle.net/1959.13/927259

Chicago Manual of Style (16^th Edition):

Roy, Tammie K. “Matrix metalloproteinase-2 in early embryo implantation in the mouse.” 2011. Doctoral Dissertation, University of Newcastle. Accessed March 05, 2021. http://hdl.handle.net/1959.13/927259.

MLA Handbook (7^th Edition):

Roy, Tammie K. “Matrix metalloproteinase-2 in early embryo implantation in the mouse.” 2011. Web. 05 Mar 2021.

Vancouver:

Roy TK. Matrix metalloproteinase-2 in early embryo implantation in the mouse. [Internet] [Doctoral dissertation]. University of Newcastle; 2011. [cited 2021 Mar 05]. Available from: http://hdl.handle.net/1959.13/927259.

Council of Science Editors:

Roy TK. Matrix metalloproteinase-2 in early embryo implantation in the mouse. [Doctoral Dissertation]. University of Newcastle; 2011. Available from: http://hdl.handle.net/1959.13/927259

Florida Atlantic University

3. Ibrahim, Mariam. Synthesis of Fluorogenic Probes Specific for Matrix Metalloproteinase 13.

Degree: MS, 2020, Florida Atlantic University

URL: http://fau.digital.flvc.org/islandora/object/fau:44424

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Matrix Metalloproteinase-13 (MMP-13) belongs to a large family of proteolytic enzymes which are characterized by their ability to degrade the extracellular matrix components. MMP-13 appears… (more)

Subjects/Keywords: Matrix Metalloproteinases; Peptides; Fluorogenic probes

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Ibrahim, M. (2020). Synthesis of Fluorogenic Probes Specific for Matrix Metalloproteinase 13. (Masters Thesis). Florida Atlantic University. Retrieved from http://fau.digital.flvc.org/islandora/object/fau:44424

Chicago Manual of Style (16^th Edition):

Ibrahim, Mariam. “Synthesis of Fluorogenic Probes Specific for Matrix Metalloproteinase 13.” 2020. Masters Thesis, Florida Atlantic University. Accessed March 05, 2021. http://fau.digital.flvc.org/islandora/object/fau:44424.

MLA Handbook (7^th Edition):

Ibrahim, Mariam. “Synthesis of Fluorogenic Probes Specific for Matrix Metalloproteinase 13.” 2020. Web. 05 Mar 2021.

Vancouver:

Ibrahim M. Synthesis of Fluorogenic Probes Specific for Matrix Metalloproteinase 13. [Internet] [Masters thesis]. Florida Atlantic University; 2020. [cited 2021 Mar 05]. Available from: http://fau.digital.flvc.org/islandora/object/fau:44424.

Council of Science Editors:

Ibrahim M. Synthesis of Fluorogenic Probes Specific for Matrix Metalloproteinase 13. [Masters Thesis]. Florida Atlantic University; 2020. Available from: http://fau.digital.flvc.org/islandora/object/fau:44424

4. PASSADAKI, THEOKTISTI. Αξιολόγηση των επιπέδων των μεταλοπρωτεινασών στο σπερματικό πλάσμα νορμοζωοσπερμικών, ολιγοτερατοζωοσπερμικών και αζωοσπερμικών ανδρών.

Degree: 2014, Democritus University of Thrace (DUTH); Δημοκρίτειο Πανεπιστήμιο Θράκης (ΔΠΘ)

URL: http://hdl.handle.net/10442/hedi/34556

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AbstractMatrix metalloproteinases (MMPs) and the FAS/FAS ligand (FASL) system are known to play a key role in germ cell death, maintaining the immune status of… (more)

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AbstractMatrix metalloproteinases (MMPs) and the FAS/FAS ligand (FASL) system are known to play a key role in germ cell death, maintaining the immune status of the testes. MMPs belong to a large family of enzymes with the ability of tissue reconstruction. One of their roles is the release and activation of growth factors and cytokines. In human sperm, MMPs activate the function of apoptotic factors, as FASL. FASL is expressed by Sertolli cells eliminating activated T-cells that express FAS, and gametes are protected from rejection reactions. Additionally, the binding of FASL to FAS activates apoptosis during spermatogenesis, thus leading to the elimination of the abnormal gametes and the reduction of the population matching the capacity of Sertoli cells. Given the crucial role of the MMPs as well as of the FAS/FAS Ligand system in the regulation of germ cell death, we aimed to investigate the presence of the MMP-2, MMP-9 and the soluble forms of Fas (sFAS) and Fas Ligand (sFASL) in seminal plasma and their possible involvement in male infertility. For this aim, semen samples were obtained from seventy-two men (age: 38.76±9.06 years). Basic semen analysis was performed according to the WHO Laboratory Manual and seminal plasma was isolated and used for the detection of MMP-2, MMP-9, sFAS and sFASL levels. The levels of MMP-2, MMP-9, sFAS and sFASL were quantified with ELISA using immunoassay kits and correlation analysis between expression levels and semen characteristics followed. Semen analysis demonstrated that twenty four samples were considered normal, and within the abnormal six were oligozoospermic, six asthenospermic, one teratozoospermic, five oligoasthenoteratozoospermic, one asthenoteratozoospermic, fourteen oligoasthenozoospermic, two oligoteratozoospermic, six azoospermic and seven with low vitality.Concerning the MMPs, both were detected in the samples; however only for MMP-9 a quiantitative analysis was possible. There was no statistical significant difference of MMPs levels in normal and abnormal samples, however the levels of MMP-9 in abnormal samples were slightly higher. Correlation analysis showed that in abnormal samples there were statistically significant correlations with sperm vitality, slow motility, immotile sperm and time to liquefaction. sFAS and sFASL were detected in both normal and abnormal samples, and their levels in abnormal samples were slightly higher, but not statistically significant. Correlation analysis showed that sFAS correlated negatively with pH in both normal (R = - 0,49) and abnormal samples (R = - 0,36). In normal samples, sFAS was strongly positively correlated with sperm concentration (R = 0,60). Furthermore, in abnormal samples, sFAS concentrations were strongly correlated with those of sFASL (Spearman R = 0,40; p<0.001).Concluding, both factors of FAS/FASL system have been detected in seminal plasma.

Οι μεταλλοπρωτεΐνάσες (MMPs), όπως επίσης το σύστημα της πρωτεΐνης FAS και του συνδέτη της (FAS Ligand - FASL) έχουν αποδειχθεί ότι παίζουν έναν ιδιαίτερα κομβικό…

Subjects/Keywords: Μεταλλοπρωτεϊνάσες; MATRIX METALLOPROTEINASES INHIBITORS

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

PASSADAKI, T. (2014). Αξιολόγηση των επιπέδων των μεταλοπρωτεινασών στο σπερματικό πλάσμα νορμοζωοσπερμικών, ολιγοτερατοζωοσπερμικών και αζωοσπερμικών ανδρών. (Thesis). Democritus University of Thrace (DUTH); Δημοκρίτειο Πανεπιστήμιο Θράκης (ΔΠΘ). Retrieved from http://hdl.handle.net/10442/hedi/34556

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

PASSADAKI, THEOKTISTI. “Αξιολόγηση των επιπέδων των μεταλοπρωτεινασών στο σπερματικό πλάσμα νορμοζωοσπερμικών, ολιγοτερατοζωοσπερμικών και αζωοσπερμικών ανδρών.” 2014. Thesis, Democritus University of Thrace (DUTH); Δημοκρίτειο Πανεπιστήμιο Θράκης (ΔΠΘ). Accessed March 05, 2021. http://hdl.handle.net/10442/hedi/34556.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

PASSADAKI, THEOKTISTI. “Αξιολόγηση των επιπέδων των μεταλοπρωτεινασών στο σπερματικό πλάσμα νορμοζωοσπερμικών, ολιγοτερατοζωοσπερμικών και αζωοσπερμικών ανδρών.” 2014. Web. 05 Mar 2021.

Vancouver:

PASSADAKI T. Αξιολόγηση των επιπέδων των μεταλοπρωτεινασών στο σπερματικό πλάσμα νορμοζωοσπερμικών, ολιγοτερατοζωοσπερμικών και αζωοσπερμικών ανδρών. [Internet] [Thesis]. Democritus University of Thrace (DUTH); Δημοκρίτειο Πανεπιστήμιο Θράκης (ΔΠΘ); 2014. [cited 2021 Mar 05]. Available from: http://hdl.handle.net/10442/hedi/34556.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

PASSADAKI T. Αξιολόγηση των επιπέδων των μεταλοπρωτεινασών στο σπερματικό πλάσμα νορμοζωοσπερμικών, ολιγοτερατοζωοσπερμικών και αζωοσπερμικών ανδρών. [Thesis]. Democritus University of Thrace (DUTH); Δημοκρίτειο Πανεπιστήμιο Θράκης (ΔΠΘ); 2014. Available from: http://hdl.handle.net/10442/hedi/34556

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Adelaide

5. Stansborough, Romany Laura. Intestinal microvascular changes and matrix metalloproteinases in radiotherapy-induced gastrointestinal toxicity.

Degree: 2018, University of Adelaide

URL: http://hdl.handle.net/2440/118212

► Radiotherapy-induced gastrointestinal toxicity (RIGT) involves damage to the gastrointestinal mucosa and is associated with symptoms including but not limited to, diarrhoea, pain, and rectal bleeding.… (more)

▼ Radiotherapy-induced gastrointestinal toxicity (RIGT) involves damage to the gastrointestinal mucosa and is associated with symptoms including but not limited to, diarrhoea, pain, and rectal bleeding. Members of the matrix metalloproteinase (MMP) family have recently been identified as being upregulated in RIGT. Furthermore, the microvasculature has long been implicated in the development of toxicities following radiotherapy, however, the mechanisms behind this are yet to be explored. This thesis aimed to assess the microvascular response to irradiation, to further elucidate the role of MMPs in RIGT, and to assess the effect of MMP inhibition on microvascular endothelium following irradiation. This thesis consists of a general introduction, published literature review, three research chapters, one published and two submitted, and a general discussion. In chapter 1, the topic of this thesis is introduced, discussing the epidemiology and underlying pathobiology of RIGT. Chapter 2, a published critical review of the literature, consolidates literature on the role of MMPs, intestinal microvasculature, and vascular mediators in RIGT. This literature review surmised MMPs to be key regulators of endothelial mediators, and to play a key role in inducing damage to intestinal microvasculature following radiotherapy. The third chapter, published in Supportive Care in Cancer, utilized a Dark Agouti (DA) rat model of fractionated abdominal irradiation to assess changes to the intestinal microvasculature. A significant increase in apoptosis of microvascular cells 6 and 15 weeks from the first dose of irradiation was found, corresponding with histopathological damage and apoptosis in the jejunal and colonic crypts. This study suggested regional and timing-specific changes in the intestinal microvasculature to occur in response to fractionated radiotherapy. Chapter four assessed levels of MMPs in the jejunum and colon in the same DA rat model of RIGT. Whilst mRNA expression MMP-1, -2, and -14 significantly increased in the jejunum, only MMP-2 expression increased in the colon. MMP-2 immunostaining was also observed to be increased in both the jejunum and colon, a finding supported by western blotting, showing significantly increased MMP-2 protein levels in both the jejunum and colon at week 6. This supported a role for MMP-2 in the pathobiology of RIGT. Chapter five, the final research chapter, assessed vascular mediator expression in the DA rat model of RIGT, as well as the effects of irradiation and MMP inhibition on tumour-associated microvascular endothelial cells derived from DA rat mammary adenocarcinoma. This study confirmed an in vivo increase in the vascular mediators, VEGF, TGFβ, angiostatin, and endostatin. Cell culture results confirmed an increase in both MMP-2 and -9 following irradiation, significantly attenuated by MMP inhibition, however this attenuation did not alter the expression of vascular mediators or the toxicity profile of irradiation. In summary, this thesis contributed to the field of supportive care in… Advisors/Committee Members: Gibson, Rachel (advisor), Al-dasooqui, Noor (advisor), Bateman, Emma (advisor), Keefe, Dorothy (advisor), Adelaide Medical School (school).

Subjects/Keywords: Radiotherapy; toxicity; gastroinestinal; matrix metalloproteinases; endothelium

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Stansborough, R. L. (2018). Intestinal microvascular changes and matrix metalloproteinases in radiotherapy-induced gastrointestinal toxicity. (Thesis). University of Adelaide. Retrieved from http://hdl.handle.net/2440/118212

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Stansborough, Romany Laura. “Intestinal microvascular changes and matrix metalloproteinases in radiotherapy-induced gastrointestinal toxicity.” 2018. Thesis, University of Adelaide. Accessed March 05, 2021. http://hdl.handle.net/2440/118212.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Stansborough, Romany Laura. “Intestinal microvascular changes and matrix metalloproteinases in radiotherapy-induced gastrointestinal toxicity.” 2018. Web. 05 Mar 2021.

Vancouver:

Stansborough RL. Intestinal microvascular changes and matrix metalloproteinases in radiotherapy-induced gastrointestinal toxicity. [Internet] [Thesis]. University of Adelaide; 2018. [cited 2021 Mar 05]. Available from: http://hdl.handle.net/2440/118212.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Stansborough RL. Intestinal microvascular changes and matrix metalloproteinases in radiotherapy-induced gastrointestinal toxicity. [Thesis]. University of Adelaide; 2018. Available from: http://hdl.handle.net/2440/118212

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Brigham Young University

6. Matias Orozco, Catalina. Control of Matrix Metalloproteinases in a Periodontitis Model: Molecules That Trigger or Inhibit MMP Production.

Degree: PhD, 2016, Brigham Young University

URL: https://scholarsarchive.byu.edu/cgi/viewcontent.cgi?article=7242&context=etd

► In periodontitis, there is a disruption in the homeostasis of the oral microbiome by peridontopathogenic bacteria. However, while bacteria is essential for periodontitis to occur,… (more)

Subjects/Keywords: Matrix metalloproteinases; periodontitis; homocysteine; inflammation; Chemistry

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Matias Orozco, C. (2016). Control of Matrix Metalloproteinases in a Periodontitis Model: Molecules That Trigger or Inhibit MMP Production. (Doctoral Dissertation). Brigham Young University. Retrieved from https://scholarsarchive.byu.edu/cgi/viewcontent.cgi?article=7242&context=etd

Chicago Manual of Style (16^th Edition):

Matias Orozco, Catalina. “Control of Matrix Metalloproteinases in a Periodontitis Model: Molecules That Trigger or Inhibit MMP Production.” 2016. Doctoral Dissertation, Brigham Young University. Accessed March 05, 2021. https://scholarsarchive.byu.edu/cgi/viewcontent.cgi?article=7242&context=etd.

MLA Handbook (7^th Edition):

Matias Orozco, Catalina. “Control of Matrix Metalloproteinases in a Periodontitis Model: Molecules That Trigger or Inhibit MMP Production.” 2016. Web. 05 Mar 2021.

Vancouver:

Matias Orozco C. Control of Matrix Metalloproteinases in a Periodontitis Model: Molecules That Trigger or Inhibit MMP Production. [Internet] [Doctoral dissertation]. Brigham Young University; 2016. [cited 2021 Mar 05]. Available from: https://scholarsarchive.byu.edu/cgi/viewcontent.cgi?article=7242&context=etd.

Council of Science Editors:

Matias Orozco C. Control of Matrix Metalloproteinases in a Periodontitis Model: Molecules That Trigger or Inhibit MMP Production. [Doctoral Dissertation]. Brigham Young University; 2016. Available from: https://scholarsarchive.byu.edu/cgi/viewcontent.cgi?article=7242&context=etd

University of Manchester

7. Al Gharibi, Khalaf. MMP family protein expression as prognostic biomarkers in human soft tissue sarcoma of extremities.

Degree: PhD, 2012, University of Manchester

URL: https://www.research.manchester.ac.uk/portal/en/theses/thesis-title-mmp-family-protein-expression-as-prognostic-biomarkers-in-human-soft-tissue-sarcoma-of-extremities(c99f2355-5c2c-4a0c-bf40-3acace12c94a).html ; http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.570253

► Soft tissue sarcomas (STS) are rare human malignant neoplasms, arising mostly from stem cells within non-skeletal connective tissues. They account for approximately 1% of all… (more)

▼ Soft tissue sarcomas (STS) are rare human malignant neoplasms, arising mostly from stem cells within non-skeletal connective tissues. They account for approximately 1% of all human malignancies. Matrix metalloproteinases (MMPs) are enzymes involved in degradation of the extracellular matrix and their expression by cancer cells allows the cells to penetrate basement membranes and tissue matrix, thereby invading and metastasising. The most studied malignant tumours from the perspective of MMP expression and its relationship to malignant behaviour are epithelial-derived carcinomas. MMPs role in invasion and metastasis of sarcomas has been very little investigated. This is in part because of the difficulty in accumulating sufficient tumour tissue to enable statistically relevant analysis of sufficient tumours. The purpose of this thesis was to examine the expression of key MMPs - MMP-2, MMP-7, MMP-9, and MMP-14 and their inhibitors (TIMP-1 and TIMP-2) at the invasive/subcapsular edge of human malignant and benign connective tissue tumours using immunohistochemistry, a technique that allows a very high level of reaction product localisation within tumours. In three different STS types and appropriate benign equivalents, the expression of MMPs -2, -7, -9, and -14 and their inhibitors (TIMPs -1 and -2) were measured using intensity of staining and the percentage area of staining by image analysis. The results were compared between tumour types and against histological grading that is widely used as a prognostic factor. The findings from this research indicated that metalloproteinases were commonly expressed in STS and benign equivalents. There were differences in expression of some benign versus malignant neoplasms of the same group. No uniform pattern of expression of any of MMPs was observed across the tumours, but some of the data, most notably that for expression of MMP-2 and -9 indicate, a role for MMPs in malignant behaviour and some showed (e.g. MMPs -7 and -14) change in expression with the grade of malignant tumours in the same broad category. There is some evidence of an inverse relationship between MMP and appropriate TIMP expression suggesting that a failure of inhibition, as much as increased expression, is a feature of malignancy.

Subjects/Keywords: 616.99; Matrix Metalloproteinases; Soft Tissue Sarcoma

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Al Gharibi, K. (2012). MMP family protein expression as prognostic biomarkers in human soft tissue sarcoma of extremities. (Doctoral Dissertation). University of Manchester. Retrieved from https://www.research.manchester.ac.uk/portal/en/theses/thesis-title-mmp-family-protein-expression-as-prognostic-biomarkers-in-human-soft-tissue-sarcoma-of-extremities(c99f2355-5c2c-4a0c-bf40-3acace12c94a).html ; http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.570253

Chicago Manual of Style (16^th Edition):

Al Gharibi, Khalaf. “MMP family protein expression as prognostic biomarkers in human soft tissue sarcoma of extremities.” 2012. Doctoral Dissertation, University of Manchester. Accessed March 05, 2021. https://www.research.manchester.ac.uk/portal/en/theses/thesis-title-mmp-family-protein-expression-as-prognostic-biomarkers-in-human-soft-tissue-sarcoma-of-extremities(c99f2355-5c2c-4a0c-bf40-3acace12c94a).html ; http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.570253.

MLA Handbook (7^th Edition):

Al Gharibi, Khalaf. “MMP family protein expression as prognostic biomarkers in human soft tissue sarcoma of extremities.” 2012. Web. 05 Mar 2021.

Vancouver:

Al Gharibi K. MMP family protein expression as prognostic biomarkers in human soft tissue sarcoma of extremities. [Internet] [Doctoral dissertation]. University of Manchester; 2012. [cited 2021 Mar 05]. Available from: https://www.research.manchester.ac.uk/portal/en/theses/thesis-title-mmp-family-protein-expression-as-prognostic-biomarkers-in-human-soft-tissue-sarcoma-of-extremities(c99f2355-5c2c-4a0c-bf40-3acace12c94a).html ; http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.570253.

Council of Science Editors:

Al Gharibi K. MMP family protein expression as prognostic biomarkers in human soft tissue sarcoma of extremities. [Doctoral Dissertation]. University of Manchester; 2012. Available from: https://www.research.manchester.ac.uk/portal/en/theses/thesis-title-mmp-family-protein-expression-as-prognostic-biomarkers-in-human-soft-tissue-sarcoma-of-extremities(c99f2355-5c2c-4a0c-bf40-3acace12c94a).html ; http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.570253

8. PIGOTT, MARIA THERESE. Intestinal targeting of small molecule MMP and TGR5 ligands for inflammatory bowel disease.

Degree: School of Pharmacy & Pharma. Sciences. Discipline of Pharmacy, 2018, Trinity College Dublin

URL: http://hdl.handle.net/2262/84983

► The aetiology of inflammatory bowel disease (IBD) is incompletely understood but it is generally accepted to involve a dysregulated intestinal immune response to commensal microbiota… (more)

▼ The aetiology of inflammatory bowel disease (IBD) is incompletely understood but it is generally accepted to involve a dysregulated intestinal immune response to commensal microbiota and an increase in intestinal permeability. Matrix metalloproteinases (MMPs), MMP-9 (gelatinase B) in particular, are implicated in this compromise of intestinal barrier structure and have further roles in the potentiation and augmentation of disease. Takeda G-protein coupled receptor 5 (TGR5), a bile acid receptor, is also involved in maintenance of intestinal barrier integrity and there is evidence to suggest that both these targets are accessible to modulators confined to the gut lumen. Expressed in many tissues and cell types and involved in many physiological processes, the potential of MMP and TGR5 modulators has been limited by unwanted off-target effects. We proposed that small molecule ligands designed to be confined to the gastrointestinal lumen through judicious modification of physicochemical properties could achieve non-systemic effects. Suppression of oral absorption potential of an established gelatinase inhibitory scaffold was pursued by synthetic strategies to increase molecular weight and/or polarity including preparation of a dimer linked by a short PEG chain or introduction of a permanent charge. Bulky conjugates of an MMP inhibitor and lithocholic acid (LCA), the most potent endogenous bile acid agonist at TGR5, were also prepared. By virtue of their high molecular weight, such conjugates would be expected to have limited absorption potential and we hoped to retain activity at both targets. Compound 53, a permanently positively charged barbiturate-based gelatinase inhibitor was a potent inhibitor of recombinant human MMP-9 as measured by fluorogenic assay and an uptake assay in Caco-2 cells indicated that it was a poor candidate for passive uptake. It was chosen for evaluation in a DSS mouse model of colitis to test the hypothesis that disease modifying effects can be achieved by inhibition of apically secreted gelatinases. Compound 53 treatment reduced the severity of DSS-induced colitis in mice and the disease activity index (DAI) scores of the treated group were significantly lower than the DSS group. PCR analysis of the retained colon samples indicated that the pro-inflammatory cytokines TNF-?, IL-1? and IL-6 were downregulated at gene level in the treated group compared to the DSS control group and these downregulations were statistically significant. The MMP-9 inhibitor ? TGR5 agonist conjugates retained potent MMP-9 inhibition but activation of TGR5 showed tighter structural specificity than expected. Some compounds of the series caused accumulation of cAMP, the downstream second messenger of TGR5 activation, in human enterendocrine NCI-H716 cells but potency was much lower than LCA. It is yet unknown if this level of activity could exert a functional effect in the context of a gut confined agent in appropriate animal models. This work contributed to our knowledge of binding at the human TGR5 receptor, its… Advisors/Committee Members: Gilmer, John.

Subjects/Keywords: matrix metalloproteinases; TGR5; inflammatory bowel disease; MMPs

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

PIGOTT, M. T. (2018). Intestinal targeting of small molecule MMP and TGR5 ligands for inflammatory bowel disease. (Thesis). Trinity College Dublin. Retrieved from http://hdl.handle.net/2262/84983

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

PIGOTT, MARIA THERESE. “Intestinal targeting of small molecule MMP and TGR5 ligands for inflammatory bowel disease.” 2018. Thesis, Trinity College Dublin. Accessed March 05, 2021. http://hdl.handle.net/2262/84983.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

PIGOTT, MARIA THERESE. “Intestinal targeting of small molecule MMP and TGR5 ligands for inflammatory bowel disease.” 2018. Web. 05 Mar 2021.

Vancouver:

PIGOTT MT. Intestinal targeting of small molecule MMP and TGR5 ligands for inflammatory bowel disease. [Internet] [Thesis]. Trinity College Dublin; 2018. [cited 2021 Mar 05]. Available from: http://hdl.handle.net/2262/84983.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

PIGOTT MT. Intestinal targeting of small molecule MMP and TGR5 ligands for inflammatory bowel disease. [Thesis]. Trinity College Dublin; 2018. Available from: http://hdl.handle.net/2262/84983

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Clemson University

9. Parasaram, Vaideesh. Targeted Delivery of Drug Loaded Albumin Nanoparticles to Emphysematous Lungs to Preserve Elastin and Mitigate Matrix Metalloproteinase Activity.

Degree: PhD, Bioengineering, 2018, Clemson University

URL: https://tigerprints.clemson.edu/all_dissertations/2199

► Chronic obstructive pulmonary disease (COPD) is the third leading cause of death in U.S. following cancer and heart disease. COPD is an umbrella term for… (more)

Subjects/Keywords: COPD; Elastin; Emphysema; Matrix metalloproteinases; Pentagalloyl glucose

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APA (6^th Edition):

Parasaram, V. (2018). Targeted Delivery of Drug Loaded Albumin Nanoparticles to Emphysematous Lungs to Preserve Elastin and Mitigate Matrix Metalloproteinase Activity. (Doctoral Dissertation). Clemson University. Retrieved from https://tigerprints.clemson.edu/all_dissertations/2199

Chicago Manual of Style (16^th Edition):

Parasaram, Vaideesh. “Targeted Delivery of Drug Loaded Albumin Nanoparticles to Emphysematous Lungs to Preserve Elastin and Mitigate Matrix Metalloproteinase Activity.” 2018. Doctoral Dissertation, Clemson University. Accessed March 05, 2021. https://tigerprints.clemson.edu/all_dissertations/2199.

MLA Handbook (7^th Edition):

Parasaram, Vaideesh. “Targeted Delivery of Drug Loaded Albumin Nanoparticles to Emphysematous Lungs to Preserve Elastin and Mitigate Matrix Metalloproteinase Activity.” 2018. Web. 05 Mar 2021.

Vancouver:

Parasaram V. Targeted Delivery of Drug Loaded Albumin Nanoparticles to Emphysematous Lungs to Preserve Elastin and Mitigate Matrix Metalloproteinase Activity. [Internet] [Doctoral dissertation]. Clemson University; 2018. [cited 2021 Mar 05]. Available from: https://tigerprints.clemson.edu/all_dissertations/2199.

Council of Science Editors:

Parasaram V. Targeted Delivery of Drug Loaded Albumin Nanoparticles to Emphysematous Lungs to Preserve Elastin and Mitigate Matrix Metalloproteinase Activity. [Doctoral Dissertation]. Clemson University; 2018. Available from: https://tigerprints.clemson.edu/all_dissertations/2199

10. Hodges, Deborah Jane. Biophysical studies of TIMP-1.

Degree: PhD, 1995, Open University

URL: http://oro.open.ac.uk/57546/ ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.282777

► This study had two aspects. The first was the production and purification of TIMP-l. The second was a series of biophysical studies of TIMP-l and… (more)

▼ This study had two aspects. The first was the production and purification of TIMP-l. The second was a series of biophysical studies of TIMP-l and a TIMP-l derived peptide. A monoclonal antibody affinity column was developed and used to purifY large quantities of human TIMP-l for further experiments. Two E.coli expression systems were studied to determine whether they would be suitable for large scale production of recombinant protein. In the first system TIMP-I was to be secreted as a fusion protein which could be cleaved, leaving a free N-terminus. It was discovered that it was not possible to cleave off the fusion protein. In the second system, the protein was secreted, without additions to the periplasm. Although active protein, with the correct N-tenninus, was obtained, the yields were too low to be of use for large scale expression. Secondary structure analysis by CD and FTIR showed TIMP-l to be a mostly f3- sheet protein (approaching 50%) with around 20% a-helix. A temperature study using these techniques found that little change occurs until temperatures of over 60°C where the protein aggregates. The small changes appear to be a general loosening of the structure. In analyses of the surface of TIMP-l, additional carbohydrate was identified (other than the two N-linked chains) using Con-A probing of Western blots. TIMP-l purified from WI-38 foetal lung fibroblast cells can be separated into two pools by Concanavalin A-Sepharose chromatography. These two pools were found to have a different set of pIs and a different monosaccharide composition. The use of NMR paramagnetic probes identified a hydrophobic region exposed on the surface of TIMP-I. This region probably includes a tyrosine residue and either a tryptophan or phenylalanine. The presence of an exposed hydrophobic region was also shown in binding studies using the fluorescent probe ANS. These studies identified a single, low affinity binding site. An additional study with the N-terminal fragment of type-I collagenase found no binding sites on the enzyme, but a change in fluorescence occurred when TIMP-I was present. A peptide was designed based on the N-terminal sequence of TIMP-I. High homology, susceptibility to mutation and an interesting resemblance to the Bowman-Birk family of inhibitors suggested that this peptide might be inhibitory. It was found to have only a weak inhibitory activity against gelatinase. NMR studies of this peptide in water showed a large number of conformers as a result of stabilisation of the cis isomer of its proline residues. This preference for the cis form was retained for one proline in the solvent, TFE. Preliminary NMR studies were also carried out which concluded that TIMP-I should be suitable for further structural studies using isotopic labelling.

Subjects/Keywords: 572; Matrix metalloproteinases

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Hodges, D. J. (1995). Biophysical studies of TIMP-1. (Doctoral Dissertation). Open University. Retrieved from http://oro.open.ac.uk/57546/ ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.282777

Chicago Manual of Style (16^th Edition):

Hodges, Deborah Jane. “Biophysical studies of TIMP-1.” 1995. Doctoral Dissertation, Open University. Accessed March 05, 2021. http://oro.open.ac.uk/57546/ ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.282777.

MLA Handbook (7^th Edition):

Hodges, Deborah Jane. “Biophysical studies of TIMP-1.” 1995. Web. 05 Mar 2021.

Vancouver:

Hodges DJ. Biophysical studies of TIMP-1. [Internet] [Doctoral dissertation]. Open University; 1995. [cited 2021 Mar 05]. Available from: http://oro.open.ac.uk/57546/ ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.282777.

Council of Science Editors:

Hodges DJ. Biophysical studies of TIMP-1. [Doctoral Dissertation]. Open University; 1995. Available from: http://oro.open.ac.uk/57546/ ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.282777

University of Missouri – Columbia

11. Tomlinson, Brittany N. Of mice and men: can MMP-9 be a biomarker and a potential target for treatment after traumatic brain injury?.

Degree: 2013, University of Missouri – Columbia

URL: http://hdl.handle.net/10355/38597

► [ACCESS RESTRICTED TO THE UNIVERSITY OF MISSOURI AT AUTHOR'S REQUEST.] Traumatic brain injury (TBI) is a highly prevalent cause of death and disability in the… (more)

Subjects/Keywords: traumatic brain injury; matrix metalloproteinases; cerebrospinal fluid

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APA (6^th Edition):

Tomlinson, B. N. (2013). Of mice and men: can MMP-9 be a biomarker and a potential target for treatment after traumatic brain injury?. (Thesis). University of Missouri – Columbia. Retrieved from http://hdl.handle.net/10355/38597

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Tomlinson, Brittany N. “Of mice and men: can MMP-9 be a biomarker and a potential target for treatment after traumatic brain injury?.” 2013. Thesis, University of Missouri – Columbia. Accessed March 05, 2021. http://hdl.handle.net/10355/38597.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Tomlinson, Brittany N. “Of mice and men: can MMP-9 be a biomarker and a potential target for treatment after traumatic brain injury?.” 2013. Web. 05 Mar 2021.

Vancouver:

Tomlinson BN. Of mice and men: can MMP-9 be a biomarker and a potential target for treatment after traumatic brain injury?. [Internet] [Thesis]. University of Missouri – Columbia; 2013. [cited 2021 Mar 05]. Available from: http://hdl.handle.net/10355/38597.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Tomlinson BN. Of mice and men: can MMP-9 be a biomarker and a potential target for treatment after traumatic brain injury?. [Thesis]. University of Missouri – Columbia; 2013. Available from: http://hdl.handle.net/10355/38597

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Florida Atlantic University

12. Tokmina-Roszyk, Dorota. THE ROLE OF MATRIX METALLOPROTEINASE-28 IN HEALTH AND DISEASE.

Degree: 2020, Florida Atlantic University

URL: http://fau.digital.flvc.org/islandora/object/fau:64722

►

Matrix Metalloproteinase-28 (MMP-28) is the newest and least characterized member of MMP family. To date several potential substrate candidates for MMP-28 have been proposed but… (more)

▼

Matrix Metalloproteinase-28 (MMP-28) is the newest and least characterized member of MMP family. To date several potential substrate candidates for MMP-28 have been proposed but no in vivo substrates for this enzyme were confirmed. In the central nervous system (CNS) MMP-28 is believed to be important factor during myelination of the developing nervous system as well as during remyelination that follows neuronal injury. On the other hand, MMP-28 has been found in actively demyelinating lesions in both experimental autoimmune encephalopathy (EAE) and multiple sclerosis patients suggesting its possible role in pathological events associated with autoimmune neurodegenerative processes. In addition, MMP-28 has been linked to modulation of immune response and activation of macrophages which presents another role of this enzyme in autoimmune pathologies. In the study described herein, MMP-28 has been shown to affect myelin composition and appearance, mitochondrial protein content, and vesicular transport proteins. Moreover, the decrease in myelin basic protein quantity observed in healthy MMP-28KO animals affected the myelin staining intensity in various brain regions including corpus callous. Cellular energetic studies did not reveal differences in mitochondrial function in MMP-28KO animals and no difference in reactive oxygen species was observed. In the EAE model, MMP-28 deletion increased the occurrence of atypical form of EAE characterized by increased inflammation of arbor vitae of the brain. In addition, MMP-28 deletion decreased the inflammatory infiltrates present in brains obtained from EAE animals. Lastly, MMP-28 has been shown to affect cellular energetics and activation of bone marrow derived macrophages during the initial stages and after 24 h activation. In addition, MMP-28 deletion increased proinflammatory cytokines and receptors CD86 and iNOS found in M1 polarized macrophages.

2020

Degree granted: Dissertation (Ph.D.) – Florida Atlantic University, 2020.

Collection: FAU

Advisors/Committee Members: Fields, Gregg B. (Thesis advisor), Florida Atlantic University (Degree grantor), Department of Chemistry and Biochemistry, Charles E. Schmidt College of Science.

Subjects/Keywords: Matrix Metalloproteinases; Multiple sclerosis; Neurodegenerative disease

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Tokmina-Roszyk, D. (2020). THE ROLE OF MATRIX METALLOPROTEINASE-28 IN HEALTH AND DISEASE. (Thesis). Florida Atlantic University. Retrieved from http://fau.digital.flvc.org/islandora/object/fau:64722

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Tokmina-Roszyk, Dorota. “THE ROLE OF MATRIX METALLOPROTEINASE-28 IN HEALTH AND DISEASE.” 2020. Thesis, Florida Atlantic University. Accessed March 05, 2021. http://fau.digital.flvc.org/islandora/object/fau:64722.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Tokmina-Roszyk, Dorota. “THE ROLE OF MATRIX METALLOPROTEINASE-28 IN HEALTH AND DISEASE.” 2020. Web. 05 Mar 2021.

Vancouver:

Tokmina-Roszyk D. THE ROLE OF MATRIX METALLOPROTEINASE-28 IN HEALTH AND DISEASE. [Internet] [Thesis]. Florida Atlantic University; 2020. [cited 2021 Mar 05]. Available from: http://fau.digital.flvc.org/islandora/object/fau:64722.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Tokmina-Roszyk D. THE ROLE OF MATRIX METALLOPROTEINASE-28 IN HEALTH AND DISEASE. [Thesis]. Florida Atlantic University; 2020. Available from: http://fau.digital.flvc.org/islandora/object/fau:64722

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Bradford

13. Youssef, Ahmed Mohamed Mohamed. Pharmacological investigations into matrix metalloproteinase-activated anti-tumour prodrugs : in vitro metabolic and pharmacological investigations into a series of colchicine-based peptide prodrugs activated by tumour-expressed matrix metalloproteinases.

Degree: PhD, 2014, University of Bradford

URL: http://hdl.handle.net/10454/13982

► Matrix metalloproteinases (MMPs) play a significant role in degrading the extracellular matrix in cancer development and metastasis. Overexpression of matrix metalloproteinases in tumour tissues relative… (more)

Subjects/Keywords: 616.99; Matrix Metalloproteinases; Membrane Type Matrix Metalloproteinases; Cancer; Prodrug; Drug metabolism; Colchicine; Peptide-based therapeutics

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Youssef, A. M. M. (2014). Pharmacological investigations into matrix metalloproteinase-activated anti-tumour prodrugs : in vitro metabolic and pharmacological investigations into a series of colchicine-based peptide prodrugs activated by tumour-expressed matrix metalloproteinases. (Doctoral Dissertation). University of Bradford. Retrieved from http://hdl.handle.net/10454/13982

Chicago Manual of Style (16^th Edition):

Youssef, Ahmed Mohamed Mohamed. “Pharmacological investigations into matrix metalloproteinase-activated anti-tumour prodrugs : in vitro metabolic and pharmacological investigations into a series of colchicine-based peptide prodrugs activated by tumour-expressed matrix metalloproteinases.” 2014. Doctoral Dissertation, University of Bradford. Accessed March 05, 2021. http://hdl.handle.net/10454/13982.

MLA Handbook (7^th Edition):

Youssef, Ahmed Mohamed Mohamed. “Pharmacological investigations into matrix metalloproteinase-activated anti-tumour prodrugs : in vitro metabolic and pharmacological investigations into a series of colchicine-based peptide prodrugs activated by tumour-expressed matrix metalloproteinases.” 2014. Web. 05 Mar 2021.

Vancouver:

Youssef AMM. Pharmacological investigations into matrix metalloproteinase-activated anti-tumour prodrugs : in vitro metabolic and pharmacological investigations into a series of colchicine-based peptide prodrugs activated by tumour-expressed matrix metalloproteinases. [Internet] [Doctoral dissertation]. University of Bradford; 2014. [cited 2021 Mar 05]. Available from: http://hdl.handle.net/10454/13982.

Council of Science Editors:

Youssef AMM. Pharmacological investigations into matrix metalloproteinase-activated anti-tumour prodrugs : in vitro metabolic and pharmacological investigations into a series of colchicine-based peptide prodrugs activated by tumour-expressed matrix metalloproteinases. [Doctoral Dissertation]. University of Bradford; 2014. Available from: http://hdl.handle.net/10454/13982

University of Alberta

14. Lee, Ji Won. Dual role of matrix metalloproteinases-2 in thoracic aortic aneurysm.

Degree: MS, Department of Physiology, 2013, University of Alberta

URL: https://era.library.ualberta.ca/files/1r66j214h

► With matrix metalloproteinases-2 (MMP2) deficiency, we observed thoracic aortic aneurysm in 70% of MMP2-/- mice but no abdominal aortic aneurysm following four weeks of angiotensin… (more)

Subjects/Keywords: Extracellular matrix; Aortic aneurysm; Matrix metalloproteinases; vascular remodeling

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Lee, J. W. (2013). Dual role of matrix metalloproteinases-2 in thoracic aortic aneurysm. (Masters Thesis). University of Alberta. Retrieved from https://era.library.ualberta.ca/files/1r66j214h

Chicago Manual of Style (16^th Edition):

Lee, Ji Won. “Dual role of matrix metalloproteinases-2 in thoracic aortic aneurysm.” 2013. Masters Thesis, University of Alberta. Accessed March 05, 2021. https://era.library.ualberta.ca/files/1r66j214h.

MLA Handbook (7^th Edition):

Lee, Ji Won. “Dual role of matrix metalloproteinases-2 in thoracic aortic aneurysm.” 2013. Web. 05 Mar 2021.

Vancouver:

Lee JW. Dual role of matrix metalloproteinases-2 in thoracic aortic aneurysm. [Internet] [Masters thesis]. University of Alberta; 2013. [cited 2021 Mar 05]. Available from: https://era.library.ualberta.ca/files/1r66j214h.

Council of Science Editors:

Lee JW. Dual role of matrix metalloproteinases-2 in thoracic aortic aneurysm. [Masters Thesis]. University of Alberta; 2013. Available from: https://era.library.ualberta.ca/files/1r66j214h

University of Texas Southwestern Medical Center

15. Zhou, Chengxin. The Functional Roles of Rho-Kinase and Matrix Metalloproteinases in Regulating Corneal Stromal Cell Mechanics in 3-D Collagen Matrices.

Degree: 2013, University of Texas Southwestern Medical Center

URL: http://hdl.handle.net/2152.5/2733

►

The file named "ZHOU-DISSERTATION-2013.pdf" is the primary dissertation file. Eleven (11) supplemental movie files are also available.

The main focus of my research has been… (more)

▼

The file named "ZHOU-DISSERTATION-2013.pdf" is the primary dissertation file. Eleven (11) supplemental movie files are also available.

The main focus of my research has been on understanding the biomechanical and biochemical mechanisms of cell-extracellular matrix (ECM) interactions during corneal wound healing, which may allow the development of new therapeutic strategies to promote corneal regeneration. Previous studies have established that the Rho GTPases play a central role in regulating the cytoskeletal changes associated with cell mechanical activity. A novel force monitoring system was successfully developed to investigate the role of Rho in corneal cell force generation in 3-D collagen matrices. Maximum tractional force generated by 9 million corneal fibroblasts in serum culture was around 265 Dynes. Inhibition of Rho kinase by Y-27632 induced a 69% force reduction. These results demonstrated that Rho/Rho kinase play a key role in mediating contractile force generation of corneal stromal fibroblasts in serum culture. I also investigated the functions of Rho GTPase signaling in corneal stromal fibroblast migration and cell-ECM interactions using a 3-D nested matrix construct. The experimental results showed that both the amount and the speed of corneal fibroblast migration and local collagen matrix reorganization were significantly inhibited by Y-27632. Following the inhibition, cells extended thinner dendritic processes into the outer matrix, and generated tractional forces at their leading edge. However, cells were unable to generate contractile forces needed to retract their tail and pull the cell body forward through the collagen matrix. I also studied the role of Matrix metalloproteinases (MMPs) in corneal cell mechanics, since these have been recognized as an influential component in extracellular matrix turnover and corneal repair. I first assessed the expression and collagenolytic activities of MMPs by primary corneal keratocyte in response to different signaling factors. I then studied the functions of MMPs in regulating keratocyte migration, cell-induced matrix contraction, and cell protrusive activity in 3-D collagen matrices. This study suggested that, in serum free PDGF culture, although collagenolysis was limited to a pericellular scale, primary corneal keratocytes utilized MMPs to facilitate cell migration, ECM contraction, cell spreading in 3-D collagen matrices. Thus MMPs may play a key role in facilitating cell-collagen matrix interactions by corneal keratocytes, without producing widespread disruption of corneal ECM structure.

Advisors/Committee Members: Grinnell, Frederick, Petroll, W. Matthew, Luby-Phelps, Katherine, Tang, Liping, Alexandrakis, Georgios.

Subjects/Keywords: Cell Movement; Collagen Type I; Extracellular Matrix; Matrix Metalloproteinases; Stromal Cells

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Zhou, C. (2013). The Functional Roles of Rho-Kinase and Matrix Metalloproteinases in Regulating Corneal Stromal Cell Mechanics in 3-D Collagen Matrices. (Thesis). University of Texas Southwestern Medical Center. Retrieved from http://hdl.handle.net/2152.5/2733

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Zhou, Chengxin. “The Functional Roles of Rho-Kinase and Matrix Metalloproteinases in Regulating Corneal Stromal Cell Mechanics in 3-D Collagen Matrices.” 2013. Thesis, University of Texas Southwestern Medical Center. Accessed March 05, 2021. http://hdl.handle.net/2152.5/2733.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Zhou, Chengxin. “The Functional Roles of Rho-Kinase and Matrix Metalloproteinases in Regulating Corneal Stromal Cell Mechanics in 3-D Collagen Matrices.” 2013. Web. 05 Mar 2021.

Vancouver:

Zhou C. The Functional Roles of Rho-Kinase and Matrix Metalloproteinases in Regulating Corneal Stromal Cell Mechanics in 3-D Collagen Matrices. [Internet] [Thesis]. University of Texas Southwestern Medical Center; 2013. [cited 2021 Mar 05]. Available from: http://hdl.handle.net/2152.5/2733.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Zhou C. The Functional Roles of Rho-Kinase and Matrix Metalloproteinases in Regulating Corneal Stromal Cell Mechanics in 3-D Collagen Matrices. [Thesis]. University of Texas Southwestern Medical Center; 2013. Available from: http://hdl.handle.net/2152.5/2733

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Manchester

16. Brooke, Heather. MATRIX METALLOPROTEINASES AND EXPERIMENTAL DIABETIC NEUROPATHY.

Degree: 2011, University of Manchester

URL: http://www.manchester.ac.uk/escholar/uk-ac-man-scw:106368

► Diabetic symmetrical polyneuropathy is the most common secondary complication of diabetes, with no effective treatment, apart from maintaining tight glycemic control. It is therefore essential… (more)

▼ Diabetic symmetrical polyneuropathy is the most common secondary complication of diabetes, with no effective treatment, apart from maintaining tight glycemic control. It is therefore essential to understand the mechanisms underlying the pathogenesis of the disease in order to develop new therapeutic strategies. Biochemical and structural changes are observed in the extracellular matrix (ECM) of the peripheral nerve in diabetes: including increased endoneurial collagen; reduplication of basement membranes around endoneurial capillaries; a thickening of basal lamina; and accumulation of advanced glycation end-products (AGEs). In normal nerves, ischaemic or other damage to distal axons provokes a regenerative response; in diabetes this is abortive and failure of axonal regeneration is a hallmark of clinical and experimental diabetic neuropathy. Matrix metalloproteinases (MMPs) are a large family of zinc-dependent proteolytic enzymes that cleave the protein components of the ECM. MMP-2 and MMP-9 play a central role in Wallerian degeneration and regeneration following nerve injury. This thesis investigates whether MMP-2 and -9 expression and/or activity were altered in the peripheral nerve in diabetes, and could contribute to regenerative failure in diabetic neuropathy. Using an experimental model of diabetes, we have demonstrated that MMP-2, but not MMP-9, is upregulated at gene, protein and activity levels in the rat sciatic nerve 8 weeks post-streptozotocin (STZ). This upregulation was not maintained at later time-points of diabetes. In vitro sciatic nerve cryoculture studies showed that peripheral nerve from STZ-diabetic rats was less supportive for neurite outgrowth from dissociated adult rat sensory neurons than nerve obtained from age-matched control rats. Cyrocultures were pre-treated with either MMP-2 or chondroitinase ABC, remodelling the peripheral nerve ECM, via the removal of inhibitory chondroitin sulfate proteoglycans from the sciatic nerve, and significantly enhanced its ability to support axonal regeneration, and partially restored the diabetes-associated regenerative deficit. However, exogenous MMP-2 or MMP-9 did not directly affect neurite outgrowth of dissociated adult rat sensory neurons. Finally, we assessed the neuroprotective effects of the AGE inhibitors LR90 and pyridoxamine in experimental diabetes, using a number of electrophysiological, behavioural and biochemical endpoints. These inhibitors were effective at preventing the development of some of the functional deficits observed in STZ-diabetes. Sensory nerve conduction velocity deficits and lipid peroxidation in the sciatic nerve were prevented by both LR90 and pyridoxamine. These agents have potential for the treatment of diabetic neuropathy. Advisors/Committee Members: ROTHWELL, NANCY NJ, TOMLINSON, DAVID DR, Rothwell, Nancy, Tomlinson, David, Gardiner, Natalie.

Subjects/Keywords: Diabetic neuropathy; Matrix Metalloproteinases; Extracellular matrix; Advanced glycation end-products

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Brooke, H. (2011). MATRIX METALLOPROTEINASES AND EXPERIMENTAL DIABETIC NEUROPATHY. (Doctoral Dissertation). University of Manchester. Retrieved from http://www.manchester.ac.uk/escholar/uk-ac-man-scw:106368

Chicago Manual of Style (16^th Edition):

Brooke, Heather. “MATRIX METALLOPROTEINASES AND EXPERIMENTAL DIABETIC NEUROPATHY.” 2011. Doctoral Dissertation, University of Manchester. Accessed March 05, 2021. http://www.manchester.ac.uk/escholar/uk-ac-man-scw:106368.

MLA Handbook (7^th Edition):

Brooke, Heather. “MATRIX METALLOPROTEINASES AND EXPERIMENTAL DIABETIC NEUROPATHY.” 2011. Web. 05 Mar 2021.

Vancouver:

Brooke H. MATRIX METALLOPROTEINASES AND EXPERIMENTAL DIABETIC NEUROPATHY. [Internet] [Doctoral dissertation]. University of Manchester; 2011. [cited 2021 Mar 05]. Available from: http://www.manchester.ac.uk/escholar/uk-ac-man-scw:106368.

Council of Science Editors:

Brooke H. MATRIX METALLOPROTEINASES AND EXPERIMENTAL DIABETIC NEUROPATHY. [Doctoral Dissertation]. University of Manchester; 2011. Available from: http://www.manchester.ac.uk/escholar/uk-ac-man-scw:106368

University of Manchester

17. Driscoll, Heather. Matrix metalloproteinases and experimental diabetic neuropathy.

Degree: PhD, 2011, University of Manchester

URL: https://www.research.manchester.ac.uk/portal/en/theses/matrix-metalloproteinases-and-experimental-diabetic-neuropathy(c4be55b6-7882-42f5-8c92-6900b402898c).html ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.764195

► Diabetic symmetrical polyneuropathy is the most common secondary complication of diabetes, with no effective treatment, apart from maintaining tight glycemic control. It is therefore essential… (more)

▼ Diabetic symmetrical polyneuropathy is the most common secondary complication of diabetes, with no effective treatment, apart from maintaining tight glycemic control. It is therefore essential to understand the mechanisms underlying the pathogenesis of the disease in order to develop new therapeutic strategies. Biochemical and structural changes are observed in the extracellular matrix (ECM) of the peripheral nerve in diabetes: including increased endoneurial collagen; reduplication of basement membranes around endoneurial capillaries; a thickening of basal lamina; and accumulation of advanced glycation end-products (AGEs). In normal nerves, ischaemic or other damage to distal axons provokes a regenerative response; in diabetes this is abortive and failure of axonal regeneration is a hallmark of clinical and experimental diabetic neuropathy. Matrix metalloproteinases (MMPs) are a large family of zinc-dependent proteolytic enzymes that cleave the protein components of the ECM. MMP-2 and MMP-9 play a central role in Wallerian degeneration and regeneration following nerve injury. This thesis investigates whether MMP-2 and -9 expression and/or activity were altered in the peripheral nerve in diabetes, and could contribute to regenerative failure in diabetic neuropathy. Using an experimental model of diabetes, we have demonstrated that MMP-2, but not MMP-9, is upregulated at gene, protein and activity levels in the rat sciatic nerve 8 weeks post-streptozotocin (STZ). This upregulation was not maintained at later time-points of diabetes. In vitro sciatic nerve cryoculture studies showed that peripheral nerve from STZ-diabetic rats was less supportive for neurite outgrowth from dissociated adult rat sensory neurons than nerve obtained from age-matched control rats. Cyrocultures were pre-treated with either MMP-2 or chondroitinase ABC, remodelling the peripheral nerve ECM, via the removal of inhibitory chondroitin sulfate proteoglycans from the sciatic nerve, and significantly enhanced its ability to support axonal regeneration, and partially restored the diabetes-associated regenerative deficit. However, exogenous MMP-2 or MMP-9 did not directly affect neurite outgrowth of dissociated adult rat sensory neurons. Finally, we assessed the neuroprotective effects of the AGE inhibitors LR90 and pyridoxamine in experimental diabetes, using a number of electrophysiological, behavioural and biochemical endpoints. These inhibitors were effective at preventing the development of some of the functional deficits observed in STZ-diabetes. Sensory nerve conduction velocity deficits and lipid peroxidation in the sciatic nerve were prevented by both LR90 and pyridoxamine. These agents have potential for the treatment of diabetic neuropathy.

Subjects/Keywords: 570; Advanced glycation end-products; Extracellular matrix; Diabetic neuropathy; Matrix Metalloproteinases

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❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Driscoll, H. (2011). Matrix metalloproteinases and experimental diabetic neuropathy. (Doctoral Dissertation). University of Manchester. Retrieved from https://www.research.manchester.ac.uk/portal/en/theses/matrix-metalloproteinases-and-experimental-diabetic-neuropathy(c4be55b6-7882-42f5-8c92-6900b402898c).html ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.764195

Chicago Manual of Style (16^th Edition):

Driscoll, Heather. “Matrix metalloproteinases and experimental diabetic neuropathy.” 2011. Doctoral Dissertation, University of Manchester. Accessed March 05, 2021. https://www.research.manchester.ac.uk/portal/en/theses/matrix-metalloproteinases-and-experimental-diabetic-neuropathy(c4be55b6-7882-42f5-8c92-6900b402898c).html ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.764195.

MLA Handbook (7^th Edition):

Driscoll, Heather. “Matrix metalloproteinases and experimental diabetic neuropathy.” 2011. Web. 05 Mar 2021.

Vancouver:

Driscoll H. Matrix metalloproteinases and experimental diabetic neuropathy. [Internet] [Doctoral dissertation]. University of Manchester; 2011. [cited 2021 Mar 05]. Available from: https://www.research.manchester.ac.uk/portal/en/theses/matrix-metalloproteinases-and-experimental-diabetic-neuropathy(c4be55b6-7882-42f5-8c92-6900b402898c).html ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.764195.

Council of Science Editors:

Driscoll H. Matrix metalloproteinases and experimental diabetic neuropathy. [Doctoral Dissertation]. University of Manchester; 2011. Available from: https://www.research.manchester.ac.uk/portal/en/theses/matrix-metalloproteinases-and-experimental-diabetic-neuropathy(c4be55b6-7882-42f5-8c92-6900b402898c).html ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.764195

18. Al-Dasooqi, Noor. Chemotherapy-induced mucositis : the role of matrix metalloproteinases and the extracellular matrix.

Degree: 2012, University of Adelaide

URL: http://hdl.handle.net/2440/72769

► Chemotherapeutic agents, including irinotecan hydrochloride, are highly effective in the treatment of a range of cancers; however, they cause a variety of unwanted toxicities. Mucositis… (more)

▼ Chemotherapeutic agents, including irinotecan hydrochloride, are highly effective in the treatment of a range of cancers; however, they cause a variety of unwanted toxicities. Mucositis is the term used to describe the damage caused by cytotoxic agents to mucous membranes of the alimentary tract (AT). This condition affects 40-100% of patients depending on dose regimen. There is currently no effective treatment and the underlying molecular mechanisms are not fully understood. Previous research has shown that mucositis encompasses changes in stress response gene expression and subsequently activation of tissue injury and inflammation mediators. Matrix metalloproteinases (MMPs) are a group of zinc-dependent endopeptidases; which have been shown to play a role in tissue injury and inflammation in many gastrointestinal disorders. Furthermore, MMPs mediate these phenomena through the regulation of the extracellular matrix (ECM). This work aims to elucidate whether MMPs contribute to the pathogenesis of mucositis and whether these can be used as biomarkers for mucositis development or be targeted for future treatment strategies. To investigate these aims, studies were performed in an animal model of irinotecan-induced mucositis. A pilot clinical study was also conducted. To investigate the role of MMPs in mucositis pathogenesis, a time-course model of irinotecan-induced mucositis was utilised. Rats were administered with 200mg/kg irinotecan intraperitoneally at 0h and killed 30, 60, 90 min, 2, 6, 12, 24, 48, 72, 144h post- treatment. Sections were embedded in paraffin or frozen for further analysis. To ensure the accuracy of the molecular investigations in this thesis, the appropriateness of a range of housekeeping genes for normalisation of RT-PCR methods was investigated for the first time in this model. Findings indicated that the most suitable combination of genes to use is Ywhaz/UBC in the jejunum and UBC/β-actin in the colon or UBC if restricted to a single housekeeping gene. Subsequent molecular and histological assessments demonstrated a significant alteration in gene expression and tissue levels of MMPs and their inhibitors (TIMPs) following irinotecan (p˂0.05). The increase in MMP-2, -3, -9 and -12 levels was associated with inflammatory infiltrate and maximum tissue damage. In contrast, MMP-1 expression correlated with tissue restitution. Furthermore, histological techniques illustrated a substantial increase in total collagen deposits around crypts from 24h in the jejunum and colon. Fibronectin expression decreased significantly in both regions from 6-24h following treatment. Irinotecan induced a significant alteration in epithelial cell kinetics in both the jejunum and colon (p˂0.05) and this correlated with changes in ECM components. To determine if systemic MMP levels are useful markers of impending toxicity, a pilot clinical study was carried out. Eight patients receiving a variety of chemotherapy regimens were recruited. The most reported toxicity following treatment was diarrhoea. Analysis of patient… Advisors/Committee Members: Gibson, Rachel Jane (advisor), Bowen, Joanne Marie (advisor), Dorothy Mary Kate (advisor), School of Medicine (school).

Subjects/Keywords: mucositis; chemotherapy; matrix metalloproteinases; metalloproteinases

…147 Serum matrix metalloproteinases as biomarkers of mucositis 148 6.8 6.9 Conclusions… …Matrix metalloproteinases (MMPs) are a group of zinc-dependent endopeptidases; which… …RM, Stringer A & Keefe DM (2010). Matrix metalloproteinases are possible… …x28;2009). Matrix metalloproteinases: key regulators in the pathogenesis of chemotherapy… …induced mucositis 133 6.3 Housekeeping genes in mucositis 136 6.4 Gastrointestinal matrix…

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Al-Dasooqi, N. (2012). Chemotherapy-induced mucositis : the role of matrix metalloproteinases and the extracellular matrix. (Thesis). University of Adelaide. Retrieved from http://hdl.handle.net/2440/72769

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Al-Dasooqi, Noor. “Chemotherapy-induced mucositis : the role of matrix metalloproteinases and the extracellular matrix.” 2012. Thesis, University of Adelaide. Accessed March 05, 2021. http://hdl.handle.net/2440/72769.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Al-Dasooqi, Noor. “Chemotherapy-induced mucositis : the role of matrix metalloproteinases and the extracellular matrix.” 2012. Web. 05 Mar 2021.

Vancouver:

Al-Dasooqi N. Chemotherapy-induced mucositis : the role of matrix metalloproteinases and the extracellular matrix. [Internet] [Thesis]. University of Adelaide; 2012. [cited 2021 Mar 05]. Available from: http://hdl.handle.net/2440/72769.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Al-Dasooqi N. Chemotherapy-induced mucositis : the role of matrix metalloproteinases and the extracellular matrix. [Thesis]. University of Adelaide; 2012. Available from: http://hdl.handle.net/2440/72769

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

19. Luczka, Emilie. Implication de la protéine Zonula Ocludens-2 (ZO-2) dans le processus d'invasion tumorale : Implication of Zonula Occludens-2 protein (ZO-2) in the tumor invasion process.

Degree: Docteur es, Médecine, 2011, Reims

URL: http://www.theses.fr/2011REIMM205

►

Lors de l’invasion tumorale, les cellules épithéliales tumorales acquièrent des propriétésmigratoires et invasives impliquant des modifications phénotypiques importantes. Parmi ceschangements, on observe notamment une réorganisation… (more)

▼

Lors de l’invasion tumorale, les cellules épithéliales tumorales acquièrent des propriétésmigratoires et invasives impliquant des modifications phénotypiques importantes. Parmi ceschangements, on observe notamment une réorganisation ou une perte des complexes d’adhérenceintercellulaire et une acquisition de la capacité à dégrader la matrice extracellulaire à travers uneaugmentation d’expression des métalloprotéinases matricielles (MMPs). Dans cette étude, nous noussommes plus particulièrement intéressés aux jonctions serrées qui sont constituées de protéinestransmembranaires (occludine, claudines) liées au cytosquelette d’actine par des protéinescytoplasmiques sous-membranaires incluant les Zonula Occludens (ZO-1, -2 et -3). Parmi cesmolécules, nous avons évalué le rôle potentiel de ZO-2 dans l’acquisition de propriétés invasives parles cellules tumorales. In vivo, nous avons montré une diminution d’expression des ZOs dans lescancers broncho-pulmonaires avec une localisation cytoplasmique préférentielle. De plus, in vitro, lalocalisation des ZOs varie en fonction du potentiel invasif des cellules tumorales et leur réorganisationest corrélée à la migration cellulaire. Nous démontrons également que l’inhibition de ZO-2 augmenteles capacités invasives de cellules tumorales invasives et s’accompagne d’une augmentationd’expression des MMP-2 et -14 et du facteur de transcription ZEB-2. Ces résultats suggèrent que ZO-2, composant structural des complexes d’adhérence intercellulaire dans les cellules différenciées,pourrait jouer un rôle clé dans le processus d’invasion tumorale. Sa capacité à transiter de lamembrane au cytoplasme et/ou au noyau lui permettrait d’agir comme une molécule de signalisationen régulant la transcription de gènes. Les données obtenues démontrent un rôle anti-invasif de ZO-2.

During tumor invasion, tumor epithelial cells acquire migratory and invasive propertiesinvolving important phenotypic alterations. Among these changes, one can observe a reorganization ora loss of cell-cell adhesion complexes such as tight junctions and an increased ability to degradeextracellular matrix through an enhanced expression of matrix metalloproteinases (MMPs). Tightjunctions are composed of transmembrane proteins (occludin, claudins) linked to the actincytoskeleton through cytoplasmic adaptor molecules including those of the zonula occludens family(ZO-1, -2, -3). Among these molecules, we evaluated the potential role of ZO-2 in the acquisition ofinvasive properties by tumor cells. In vivo, we showed a decrease of ZOs expression in bronchopulmonarycancers with a preferential localization in the cytoplasm. In addition, in vitro, thelocalization of ZOs varies according to invasive properties of tumor cells and their reorganization iscorrelated with cell migration. We also demonstrate that ZO-2 inhibition increases invasive capacitiesof invasive tumor cells. This was associated with an increase of MMPs (MMP-2 and -14) and thetranscription factor ZEB-2 expression. These results suggest that ZO-2, known as a…

Advisors/Committee Members: Gilles, Christine (thesis director), Polette, Myriam (thesis director).

Subjects/Keywords: Tumeurs; ZO-2; Matrix metalloproteinases; ZEB-2; Invasion tumorale; Neoplasms; Zonula occludens-2 protein; Neoplasm invasiveness; Matrix metalloproteinases; ZEB2 protein, human

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Luczka, E. (2011). Implication de la protéine Zonula Ocludens-2 (ZO-2) dans le processus d'invasion tumorale : Implication of Zonula Occludens-2 protein (ZO-2) in the tumor invasion process. (Doctoral Dissertation). Reims. Retrieved from http://www.theses.fr/2011REIMM205

Chicago Manual of Style (16^th Edition):

Luczka, Emilie. “Implication de la protéine Zonula Ocludens-2 (ZO-2) dans le processus d'invasion tumorale : Implication of Zonula Occludens-2 protein (ZO-2) in the tumor invasion process.” 2011. Doctoral Dissertation, Reims. Accessed March 05, 2021. http://www.theses.fr/2011REIMM205.

MLA Handbook (7^th Edition):

Luczka, Emilie. “Implication de la protéine Zonula Ocludens-2 (ZO-2) dans le processus d'invasion tumorale : Implication of Zonula Occludens-2 protein (ZO-2) in the tumor invasion process.” 2011. Web. 05 Mar 2021.

Vancouver:

Luczka E. Implication de la protéine Zonula Ocludens-2 (ZO-2) dans le processus d'invasion tumorale : Implication of Zonula Occludens-2 protein (ZO-2) in the tumor invasion process. [Internet] [Doctoral dissertation]. Reims; 2011. [cited 2021 Mar 05]. Available from: http://www.theses.fr/2011REIMM205.

Council of Science Editors:

Luczka E. Implication de la protéine Zonula Ocludens-2 (ZO-2) dans le processus d'invasion tumorale : Implication of Zonula Occludens-2 protein (ZO-2) in the tumor invasion process. [Doctoral Dissertation]. Reims; 2011. Available from: http://www.theses.fr/2011REIMM205

University of Otago

20. Kazantseva, Marina Grigorievna. Smoking, genes and inflammation .

Degree: 2012, University of Otago

URL: http://hdl.handle.net/10523/2496

► Rheumatoid arthritis (RA) is an, autoimmune disease where genetic predisposition and environmental factors increase the risk of developing RA and the severity of the disease.… (more)

▼ Rheumatoid arthritis (RA) is an, autoimmune disease where genetic predisposition and environmental factors increase the risk of developing RA and the severity of the disease. Cigarette smoking is the major recognised environmental risk factor, and there is a combined effect from smoking and the human leukocyte antigen (HLA)-DRB1 shared epitope (SE) genotype on the risk of developing RA. This study sought to establish any direct effect of smoking and/or the genetic predisposition on the inflammation driving RA and to identify biological mechanism(s) that might explain epidemiological data linking smoking, genetics and RA. The aim of initial work was to establish any involvement of aryl hydrocarbon receptor (AHR)-mediated mechanisms within inflamed rheumatoid tissues. The expression of AHR, CYP1A1 and AHRR genes were quantified by real-time PCR in twenty synovial and eighteen subcutaneous nodule tissues. Patient’s smoking status was established at the time tissue was obtained. The results show smoking causes significant AHR activation in joint synovial tissue, but not in rheumatoid nodule tissues. A subset of synovial DCs show activated AHR in smokers, consistent with the sensitivity of human mo-DCs stimulated with the AHR agonist, benzo(a)pyrene (BaP) in vitro. It is concluded that DCs within the joint synovium are the principal immune cells that respond to cigarette smoke exposure. Microarray analysis revealed that the expression of 547 synovial genes was up-regulated by smoking, including folate receptor 1 (FOLR1), matrix metalloproteinases (MMP)9, MMP11 and MMP14, TNF-superfamily members, TNFSF10/TRAIL and TNFRSF10B/TRAILR2 and transcription factors, RUNX1 and RUNX2. Cell motility and adhesion were the biological processes in synovium most affected by smoking. The expression of 307 synovial genes was down-regulated by smoking, including the vascular “protective” genes, KLF2 and eNOS, suggesting that endothelial function is affected by smoking with implications for vasculitis and the development of rheumatoid nodules associated with severe RA. Any effect of smoking and SE copy number on genes associated with AHR signalling and other immune-inflammatory genes was established. Results suggest there are solitary, reciprocal or synergistic effects from smoking and the SE in rheumatoid inflammation, which are gene dependent. Thus, smoking increases AHR activation in synovium; the SE has no effect. Furthermore, while smoking reduces IL17A expression in synovial tissue, indications are that SE copy number increases IL17A expression. In combination, smoking and the SE increase synovial MMP9 gene expression. Human promonocytic U937 cells were used to model the effect of BaP exposure on MMP9 expression. PMA-activated U937 cells acquire an ability to respond to BaP, including with increased MMP9 gene expression. AHR-specific siRNA, confirmed that AHR regulates MMP9 gene expression. Further data implicate different MMPs in rheumatoid tissue destruction. High MMP7 expression by macrophages occurs in a subset of nodule tissues.… Advisors/Committee Members: Hessian, Paul A (advisor).

Subjects/Keywords: rheumatoid arthritis; inflammation; smoking; aryl hydrocarbon receptor; matrix metalloproteinases

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❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Kazantseva, M. G. (2012). Smoking, genes and inflammation . (Doctoral Dissertation). University of Otago. Retrieved from http://hdl.handle.net/10523/2496

Chicago Manual of Style (16^th Edition):

Kazantseva, Marina Grigorievna. “Smoking, genes and inflammation .” 2012. Doctoral Dissertation, University of Otago. Accessed March 05, 2021. http://hdl.handle.net/10523/2496.

MLA Handbook (7^th Edition):

Kazantseva, Marina Grigorievna. “Smoking, genes and inflammation .” 2012. Web. 05 Mar 2021.

Vancouver:

Kazantseva MG. Smoking, genes and inflammation . [Internet] [Doctoral dissertation]. University of Otago; 2012. [cited 2021 Mar 05]. Available from: http://hdl.handle.net/10523/2496.

Council of Science Editors:

Kazantseva MG. Smoking, genes and inflammation . [Doctoral Dissertation]. University of Otago; 2012. Available from: http://hdl.handle.net/10523/2496

University of Rochester

21. Skrombolas, Denise. Development and Characterization of Protease Activated Cytokine Fusion Proteins.

Degree: PhD, 2015, University of Rochester

URL: http://hdl.handle.net/1802/29356

► Cytokines have long been known to be key immune mediators and are involved in almost all aspects of immune responses. As a result, many have… (more)

▼ Cytokines have long been known to be key immune mediators and are involved in almost all aspects of immune responses. As a result, many have been tested in a variety of cancers with some notable successes. For example, systemic IL-2 delivery is now FDA approved for the treatment of melanoma and kidney cancer. However, side effects of cytokines have greatly limited their utility as well as their efficacy. This may not be surprising given that most cytokines have evolved to act locally over short distances in an autocrine or paracrine fashion. This thesis describes a fundamentally different approach for cytokine use that more closely mimics the normal biology of cytokines but allows for their systemic delivery that could affect all metastatic tumor sites. The overall hypothesis is that by changing the balance of key cytokines at tumor sites, it is possible to alter the character of the anti-tumor immune response and ultimately affect tumor growth. These studies have focused on IL-2 and IL-12. The work in this thesis shows that these cytokines have potent effects individually but when used together in vitro exhibit even more robust effects, preferentially stimulating more cytotoxic effectors and comparatively fewer regulatory T cells. The work presented here, as well as studies in animal models and patients reveal the potential of these cytokines. Nevertheless, other studies reveal this potent activation comes with a price and side effects resulting from widespread stimulation of immune cells. To address many of these issues, this thesis describes the generation and characterization of a series of activatable fusion proteins containing the key cytokines IL-2 and IL-12. The cytokine activated fusion protein (FP) strategy consists of a cytokine linked to an inhibitory component separated by a specific protease cleavage sequence. At the tumor site, the FP can be cleaved by proteases such as Matrix Metalloproteinases (MMPs) that are over-expressed. This cleavage allows the release of the cytokine from the inhibitor and its binding to higher affinity receptors on immune cells. The data presented show that the FPs are greatly inhibited in their native form compared to the same amount of the free cytokine. Moreover, cleavage by MMPs dramatically increases their functional activity. Further, delivery of these fusion proteins engenders little inflammatory responses in vivo compared to the free cytokine. Even long-term expression of the FPs achieved using an AAV gene therapy vector results in little to no inflammatory cytokine responses as measured by luminex assays. These results indicate that the fusion proteins may be used in vivo with greatly reduced toxicity. Further, the activatable fusion protein approach represents a fundamentally different means of delivering cytokines and could serve as a platform technology to deliver any immune modulator.

Subjects/Keywords: Cytokines; Interleukin-2; Interleukin-12; Cancer Immunotherapy; Fusion Proteins; Matrix Metalloproteinases

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Skrombolas, D. (2015). Development and Characterization of Protease Activated Cytokine Fusion Proteins. (Doctoral Dissertation). University of Rochester. Retrieved from http://hdl.handle.net/1802/29356

Chicago Manual of Style (16^th Edition):

Skrombolas, Denise. “Development and Characterization of Protease Activated Cytokine Fusion Proteins.” 2015. Doctoral Dissertation, University of Rochester. Accessed March 05, 2021. http://hdl.handle.net/1802/29356.

MLA Handbook (7^th Edition):

Skrombolas, Denise. “Development and Characterization of Protease Activated Cytokine Fusion Proteins.” 2015. Web. 05 Mar 2021.

Vancouver:

Skrombolas D. Development and Characterization of Protease Activated Cytokine Fusion Proteins. [Internet] [Doctoral dissertation]. University of Rochester; 2015. [cited 2021 Mar 05]. Available from: http://hdl.handle.net/1802/29356.

Council of Science Editors:

Skrombolas D. Development and Characterization of Protease Activated Cytokine Fusion Proteins. [Doctoral Dissertation]. University of Rochester; 2015. Available from: http://hdl.handle.net/1802/29356

22. Dokko, Joana Regina. Efeito de um gel à base de Cranberry sobre a dentina submetida à erosão dentária.

Degree: Mestrado, Ortodontia e Odontologia em Saúde Coletiva, 2013, University of São Paulo

URL: http://www.teses.usp.br/teses/disponiveis/25/25144/tde-29012014-152942/ ;

►

Esta pesquisa teve como objetivo estudar in situ o efeito protetor de um gel à base de Cranberry aplicado sobre a dentina submetida à erosão.… (more)

▼

Esta pesquisa teve como objetivo estudar in situ o efeito protetor de um gel à base de Cranberry aplicado sobre a dentina submetida à erosão. Este estudo duplo cego e cruzado constou de 2 fases com duração de 5 dias cada. Para tal, 10 voluntários utilizaram2 aparelhos palatinos (um em cada fase) com 4 blocos de dentina bovina divididos em 2 grupos. Na primeira fase estiveram presentes os grupos: G1 Ação da bebida ácida (Coca-cola®) sobre a dentina bovina sem nenhum tipo de tratamento prévio; G2 - Ação da bebida ácida sobre a dentina tratada com gel à base de Cranberry e na segunda fase foi testado os grupos: G3 Ação da bebida sobre a dentina tratada com gel de aplicação tópica sem nenhum princípio ativo; G4 - Ação da bebida ácida sobre a dentina tratada previamente com gel de Clorexidina. Cada aparelho foi imerso na bebida ácida, 3x/dia, durante 5 minutos por 5 dias. A porcentagem de perda de microdureza de superfície (%PDS) e a perfilometria foram as variáveis utilizadas para quantificar as alterações dadentina. A comparação dos grupos por meio da análise de variância de medidas repetidas seguido do teste de Fisher mostrou haver diferenças estatisticamente significativas entre os grupos para o desgaste (G1: 4,98 μm ± 1,36; G2: 3,29 μm ± 1,16; G3: 4,38 μm ± 1,19; G4: 3,32 μm ± 1,55) e não apresentou diferenças entre eles na %PDS (G1: 28,12 ± 5,71; G2: 24,92 ± 5,38; G3: 25,74 ± 9,15; G4: 29,83 ± 8,63).Assim, quanto ao desgaste não houve diferença estatisticamente significativa entre os grupos controle e placebo, também não houve uma diferença entre os grupos Cranberry e clorexidina. Porém, os grupos Cranberry e clorexidina apresentaram menores valores de desgaste em relação aos grupos Placebo e Controle (sem gel), sendo esta diferença estatisticamente significativa. Os resultados obtidos nesse estudo sugerem uma significativa eficácia dos géis ativos à base de Cranberry e de Clorexidina na prevenção do desgaste da dentina submetida à erosão dentária.

The aim of this study is to evaluate the in situ effect of a Cranberry gel over dentine submitted to a erosive challenge. This crossover doble-blinded study was performed in 2 phases of 5 days each. For that purpose, 10 volunteers wore 2 palatal devices (1 for each phase) with 4 dentin specimens divided into 2 groups: First Phase: G1 - Erosive challenge (Coca-cola®) over dentine without any previous treatment; G2 - Erosive challenge over dentine previously treated with Cranberry gel; and Second Phase: G3 - Erosive challenge over dentine previously treated with a gel withot any active principle; G4 - Erosive challenge over dentine previously treated with Clorexidine gel. Each device was immersed into the acid beverage, 3 times daily for 5 minutes during 5 days. The surface microhardness change percentage (%SMC) and profilometry were be used to quantify the dentin alteratons. The comparison between groups by repeated measures analysis of variance followed by Fisher\'s test showed statistically significant differences between groups for wear…

Advisors/Committee Members: Honório, Heitor Marques.

Subjects/Keywords: Dental erosion; Dentin; Dentina; Erosão dentária; Matrix metalloproteinases; Metaloproteinases da matriz

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Dokko, J. R. (2013). Efeito de um gel à base de Cranberry sobre a dentina submetida à erosão dentária. (Masters Thesis). University of São Paulo. Retrieved from http://www.teses.usp.br/teses/disponiveis/25/25144/tde-29012014-152942/ ;

Chicago Manual of Style (16^th Edition):

Dokko, Joana Regina. “Efeito de um gel à base de Cranberry sobre a dentina submetida à erosão dentária.” 2013. Masters Thesis, University of São Paulo. Accessed March 05, 2021. http://www.teses.usp.br/teses/disponiveis/25/25144/tde-29012014-152942/ ;.

MLA Handbook (7^th Edition):

Dokko, Joana Regina. “Efeito de um gel à base de Cranberry sobre a dentina submetida à erosão dentária.” 2013. Web. 05 Mar 2021.

Vancouver:

Dokko JR. Efeito de um gel à base de Cranberry sobre a dentina submetida à erosão dentária. [Internet] [Masters thesis]. University of São Paulo; 2013. [cited 2021 Mar 05]. Available from: http://www.teses.usp.br/teses/disponiveis/25/25144/tde-29012014-152942/ ;.

Council of Science Editors:

Dokko JR. Efeito de um gel à base de Cranberry sobre a dentina submetida à erosão dentária. [Masters Thesis]. University of São Paulo; 2013. Available from: http://www.teses.usp.br/teses/disponiveis/25/25144/tde-29012014-152942/ ;

University of Oulu

23. Korpi, J. (Jarkko). Collagenase-2 (matrix metalloproteinase-8) in tongue squamous cell carcinoma, bone osteosarcoma, and wound repair.

Degree: 2010, University of Oulu

URL: http://urn.fi/urn:isbn:9789514261046

► Abstract Degradation of extracellular matrix (ECM) and basement membrane (BM) are required both in normal physiological conditions such as wound healing and in pathological tissue… (more)

▼ Abstract Degradation of extracellular matrix (ECM) and basement membrane (BM) are required both in normal physiological conditions such as wound healing and in pathological tissue remodelling such as chronic ulcers and cancers. Matrix metalloproteinases (MMPs) are an enzyme family, which can cleave most ECM and BM components. They are associated with physiological and pathological processes but their exact roles are still largely unknown. The expression of MMP-8 and MMP-26 in acute and chronic human cutaneous wounds using histological and cell culture methods were investigated. MMP-8 was expressed in epithelial cells, neutrophils, and other inflammatory cells especially in chronic ulcers while in acute wounds MMP-8 expression was weak or absent. MMP-26 was temporarily present in acute wounds while it was strongly expressed in close vicinity to the BM in multiple cell types of most chronic ulcers. In vitro keratinocyte wound assay showed that MMP-8 and -26 were expressed in migrating cells. Bone formation, collagen metabolism, and inflammation in MMP8-/- mice tooth extraction wounds and also periapical lesion formation were analysed. No differences between wild type or MMP-8-deficient mice in the new bone area or periapical lesion size were found. However, type III procollagen production was increased and inflammatory cell influx was decreased in MMP8-/- mice. In addition, Fas ligand (FasL) production was increased in mandibular alveolar mucosa but decreased in alveolar bone of MMP-8 deficient mice. MMP-8 was also found to cleave FasL in vitro. A total of 90 human mobile tongue squamous cell carcinoma (SCC) samples were collected. Bryne’s malignancy scores, thickness of the SCCs, expression of microvessel density (CD31 and factor VIII), cyclooxygenase-2 (COX-2), the laminin-5 (currently termed laminin-332) γ2-chain, integrin αvβ6, estrogen receptor-α (ER-α), estrogen receptor-β (ER-β), and MMPs (-2, -7, -8, -9, -20, and -28) were analysed. The high expression of MMP-8 was associated with a better prognosis for the patients, particularly in females. In addition, tongue carcinoma formation in MMP8-/- mice was investigated. Tongue SCC developed more often in MMP8-/- female mice than wild type littermates. In addition, MMP-8 can cleave ER- α and -β and estrogen can induce MMP-8 production in vitro. A total of 22 biopsies, 10 resection sections, and three lung metastases of 25 osteosarcoma patients samples were stained with MMP-2, -8, -13, -26, and tissue inhibitor of metalloproteinase-1 (TIMP-1) using immunohistological methods. Expression of these markers was mostly present in sarcoma cells but MMP-8 was not present in lung metastases. In resection sections, chemotherapy altered MMP-2, -8, and -13 expressions compared to biopsies. However, an association between the expression and prognosis of osteosarcoma patients could not been found. In conclusion, MMP-8 seems to be an estrogen-related protective factor in tongue SCC and can regulate ECM and BM components and inflammation during wound healing. Further studies…

Subjects/Keywords: carcinoma; squamous cell; matrix metalloproteinases; osteosarcoma; survival; wound healing

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Korpi, J. (. (2010). Collagenase-2 (matrix metalloproteinase-8) in tongue squamous cell carcinoma, bone osteosarcoma, and wound repair. (Doctoral Dissertation). University of Oulu. Retrieved from http://urn.fi/urn:isbn:9789514261046

Chicago Manual of Style (16^th Edition):

Korpi, J (Jarkko). “Collagenase-2 (matrix metalloproteinase-8) in tongue squamous cell carcinoma, bone osteosarcoma, and wound repair.” 2010. Doctoral Dissertation, University of Oulu. Accessed March 05, 2021. http://urn.fi/urn:isbn:9789514261046.

MLA Handbook (7^th Edition):

Korpi, J (Jarkko). “Collagenase-2 (matrix metalloproteinase-8) in tongue squamous cell carcinoma, bone osteosarcoma, and wound repair.” 2010. Web. 05 Mar 2021.

Vancouver:

Korpi J(. Collagenase-2 (matrix metalloproteinase-8) in tongue squamous cell carcinoma, bone osteosarcoma, and wound repair. [Internet] [Doctoral dissertation]. University of Oulu; 2010. [cited 2021 Mar 05]. Available from: http://urn.fi/urn:isbn:9789514261046.

Council of Science Editors:

Korpi J(. Collagenase-2 (matrix metalloproteinase-8) in tongue squamous cell carcinoma, bone osteosarcoma, and wound repair. [Doctoral Dissertation]. University of Oulu; 2010. Available from: http://urn.fi/urn:isbn:9789514261046

University of Oulu

24. Gäddnäs, F. (Fiia). Insights into healing response in severe sepsis from a connective tissue perspective:a longitudinal case-control study on wound healing, collagen synthesis and degradation, and matrix metalloproteinases in patients with severe sepsis.

Degree: 2010, University of Oulu

URL: http://urn.fi/urn:isbn:9789514262548

► Abstract Sepsis is a major challenge for healing responses maintaining homeostasis. Coagulation and inflammation are activated at the whole-body level, even in undamaged tissues. Despite… (more)

▼ Abstract Sepsis is a major challenge for healing responses maintaining homeostasis. Coagulation and inflammation are activated at the whole-body level, even in undamaged tissues. Despite constantly growing knowledge and advances in care, high mortality in severe sepsis remains. It was hypothesised that tissue regeneration processes may also be altered in severe sepsis. The study population consisted of 44 patients with severe sepsis and 15 healthy controls. Serum samples were obtained during ten days of severe sepsis and twice again, three months and six months later. Experimental suction blisters were performed twice during severe sepsis and at 3 and 6 months. Serum samples were obtained and suction blisters were induced once in controls. Biochemical analyses were performed to assess the level of procollagen I and III aminoterminal propeptides (PINP, PIIINP), reflecting the synthesis of corresponding collagens; in serum and suction blister fluid. In addition collagen I degradationproduct in serum was measured. Physiological measurements of transepidermal water loss and blood flow were done in order to evaluate the re-epithelisation rate and blood flow in an experimental wound. Levels of matrix metalloproteinases (MMPs) 2, 8 and 9 were measured from serum and suction blister fluid. Decrease in water evaporation from an experimental blister wound was slower in sepsis than in controls. On the fourth day the sepsis patients had higher blood flow in the blister wound than the controls (both in the healing wound and in the newly induced wound). The procollagen III aminoterminal propeptide (PIIINP) levels were increased in serum in severe sepsis, whereas procollagen I aminoterminal propeptide (PINP) levels were not, making up a pronounced PIIINP/PINP ratio. PIIINP and PINP levels were associated with disease severity and outcome. In addition, collagen I degradation measured with ICTP assay was increased in severe sepsis and PINP/ICTP ratio was lower. Furthermore, the overall protein concentration and PINP and PIIINP levels were low in suction blister fluid, which implies that the balance of the extracellular matrix consistence is disturbed in uninjured skin in severe sepsis. Then again in survivors the levels of PINP and PIIINP were up-regulated at three months but returned to normal by six months. MMP-9 levels in serum and skin blister fluid were lower in severe sepsis than in controls during the ten days studied. The MMP-2 levels were found to be increased both in serum and in skin blister fluid in severe sepsis in comparison to the controls and MMP-2 was associated with disease severity and outcome. MMP-8 was increased in serum and in skin blister fluid. In conclusion, the balance of collagen turnover is altered in severe sepsis in serum and skin and epidermal re-epithelisation is delayed. The levels of MMP-2 and MMP-8 are increased whereas levels of MMP-9 are depressed.

Subjects/Keywords: Severe sepsis; collagen; matrix metalloproteinases; procollagen propeptide; skin; suction; wound healing

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Gäddnäs, F. (. (2010). Insights into healing response in severe sepsis from a connective tissue perspective:a longitudinal case-control study on wound healing, collagen synthesis and degradation, and matrix metalloproteinases in patients with severe sepsis. (Doctoral Dissertation). University of Oulu. Retrieved from http://urn.fi/urn:isbn:9789514262548

Chicago Manual of Style (16^th Edition):

Gäddnäs, F (Fiia). “Insights into healing response in severe sepsis from a connective tissue perspective:a longitudinal case-control study on wound healing, collagen synthesis and degradation, and matrix metalloproteinases in patients with severe sepsis.” 2010. Doctoral Dissertation, University of Oulu. Accessed March 05, 2021. http://urn.fi/urn:isbn:9789514262548.

MLA Handbook (7^th Edition):

Gäddnäs, F (Fiia). “Insights into healing response in severe sepsis from a connective tissue perspective:a longitudinal case-control study on wound healing, collagen synthesis and degradation, and matrix metalloproteinases in patients with severe sepsis.” 2010. Web. 05 Mar 2021.

Vancouver:

Gäddnäs F(. Insights into healing response in severe sepsis from a connective tissue perspective:a longitudinal case-control study on wound healing, collagen synthesis and degradation, and matrix metalloproteinases in patients with severe sepsis. [Internet] [Doctoral dissertation]. University of Oulu; 2010. [cited 2021 Mar 05]. Available from: http://urn.fi/urn:isbn:9789514262548.

Council of Science Editors:

Gäddnäs F(. Insights into healing response in severe sepsis from a connective tissue perspective:a longitudinal case-control study on wound healing, collagen synthesis and degradation, and matrix metalloproteinases in patients with severe sepsis. [Doctoral Dissertation]. University of Oulu; 2010. Available from: http://urn.fi/urn:isbn:9789514262548

25. Δροσοπούλου, Κωνσταντίνα. Μηχανισμοί απόπτωσης και δράσης των μεταλλοπρωτεϊνασών στο τοίχωμα των έσω σπερματικών φλεβών σε ασθενείς με κιρσοκήλη.

Degree: 2014, University of Patras

URL: http://hdl.handle.net/10889/7632

► Η κιρσοκήλη αποτελεί τη συχνότερη αιτία ανδρικής υπογονιμότητας και ορίζεται ως η παθολογική κιρσοειδής διεύρυνση των φλεβών του σπερματικού τόνου (ελικοειδούς πλέγματος). Η επίπτωση της… (more)

▼ Η κιρσοκήλη αποτελεί τη συχνότερη αιτία ανδρικής υπογονιμότητας και ορίζεται ως η παθολογική κιρσοειδής διεύρυνση των φλεβών του σπερματικού τόνου (ελικοειδούς πλέγματος). Η επίπτωση της κιρσοκήλης στο γενικό πληθυσμό κυμαίνεται σε ποσοστό 15‐20%, ενώ στους υπογόνιμους άνδρες αγγίζει το 40%. Παρά το γεγονός ότι η κιρσοκήλη είναι σπάνια στα παιδία, πρόσφατες μελέτες έδειξαν πως το ποσοστό της αγγίζει το 6% σε παιδιά ηλικίας 10 ετών, ενώ στους εφήβους το αντίστοιχο ποσοστό ανέρχεται στο 16%. Πιθανότατα, προκαλείται λόγω ανεπάρκειας των φλεβικών βαλβίδων, κάτι που σαν αποτέλεσμα έχει την παλινδρόμηση του αίματος εντός των σπερματικών φλεβών. Η κιρσοκήλη εμφανίζεται κυρίως στην αριστερή πλευρά, εξαιτίας της ανατομίας των φλεβών στην περιοχή αυτή. Παρά την αυξημένη συχνότητα της, η παθοφυσιολογία της παραμένει εν πολλοίς άγνωστη. Πρόσφατες μελέτες σε κιρσοειδείς φλέβες κάτω άκρων έχουν δείξει ότι η μείωση του ρυθμού απόπτωσης σχετίζεται με την εμφάνιση πρωτοπαθούς φλεβίτιδας. Επιπλέον αυξημένη παραγωγή των συστατικών της εξωκυττάριας θεμέλιας ουσίας, οδηγεί στις μορφολογικές αλλοίωσεις και στην απώλεια του φλεβικού τόνου που χαρακτηρίζει τους κιρσούς κάτω άκρων. Σκοπός: Θεωρώντας ότι αντίστοιχοι μηχανισμοί πρέπει να ισχύουν όχι μόνο στους κιρσούς κάτω άκρων αλλά και στην κιρσοκήλη, υποθέσαμε ότι στο τοίχωμα των κιρσοειδών έσω σπερματικών φλεβών θα παρατηρείται κατ’ αναλογία μείωση του ρυθμού απόπτωσης και αύξηση στο ρυθμό σύνθεσης των συστατικών της εξωκυττάριας θεμέλιας ουσίας με μείωση της έκφρασης των MMPs (MMP‐1, MMP‐ 9) και αντίστοιχη αύξηση των TIMPs (TIMP‐1). Μέθοδοι: Το υλικό μελέτης αποτελούσαν 45 δείγματα έσω σπερματικών φλεβών ασθενών με κιρσοκήλη. Σαν μάρτυρες χρησιμοποιήθηκαν κλάδοι των κάτω επιγαστρικών φλεβών που αφαιρέθηκαν από τον κάθε ασθενή κατά τη διάρκεια χειρουργικής επέμβασης, έτσι ώστε ο κάθε ασθενής να αποτελεί και τον δικό του μάρτυρα. Προσδιορίσαμε τις μορφολογικές αλλοιώσεις στο αγγειακό τοίχωμα με ανοσοϊστοχημική χρώση έναντι της ακτίνης. Επιπλέον, μελετήσαμε την έκφραση των MMP‐1 και MMP‐9 καθώς και του TIMP‐1 στο τοίχωμα των κιρσοειδών όσο και των υγιών φλεβών. Τέλος, το σύνολο των δειγμάτων, εξετάσθηκαν ανοσοϊστοχημικά για την ανίχνευση των μεσολαβητών που ρυθμίζουν το ενδογενές (Bcl‐2, Cas‐9) και το εξωγενές (Cas‐8) μονοπάτι της απόπτωσης. Αποτελέσματα: Συγκριτικά με τις φυσιολογικές φλέβες, οι κιρσοειδείς εμφάνισαν πάχυνση του μέσου χιτώνα. Οι MMP‐1 και TIMP‐1 εκφράσθηκαν στο κυτταρόπλασμα των λείων μυϊκών κυττάρων του μέσου χιτώνα των αγγείων, τόσο στις φυσιολογικές όσο και στις κιρσοειδώς διευρυμένες, με την ένταση της έκφρασης όμως, να είναι μεγαλύτερη στους κιρσούς. Η MMP‐9 εντοπίσθηκε στο κυτταρόπλασμα των λείων μυϊκών κυττάρων του μέσου χιτώνα μόνο των κιρσών. Όσον αφορά την έκφραση των αποπτωτικών δεικτών, παρατηρήσαμε έκφραση της Cas‐9 στο πυρήνα των λείων μυϊκών κυττάρων του μέσου χιτώνα τόσο στις κιρσοειδείς φλέβες όσο και στους μάρτυρες, με την ένταση να είναι μεγαλύτερη στους κιρσούς. Σε μικρό αριθμό δειγμάτων παρατηρήσαμε πυρηνική… Advisors/Committee Members: Γυφτόπουλος, Κωνσταντίνος, Drosopoulou, Konstantina, Γυφτόπουλος, Κωνσταντίνος, Παπαδάκη, Ελένη, Κουρέα, Ελένη.

Subjects/Keywords: Κιρσοκήλη; Μεταλλοπρωτεϊνάσες; Απόπτωση; 616.68; Vericocele; Matrix metalloproteinases; Apoptosis

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Δροσοπούλου, �. (2014). Μηχανισμοί απόπτωσης και δράσης των μεταλλοπρωτεϊνασών στο τοίχωμα των έσω σπερματικών φλεβών σε ασθενείς με κιρσοκήλη. (Masters Thesis). University of Patras. Retrieved from http://hdl.handle.net/10889/7632

Chicago Manual of Style (16^th Edition):

Δροσοπούλου, Κωνσταντίνα. “Μηχανισμοί απόπτωσης και δράσης των μεταλλοπρωτεϊνασών στο τοίχωμα των έσω σπερματικών φλεβών σε ασθενείς με κιρσοκήλη.” 2014. Masters Thesis, University of Patras. Accessed March 05, 2021. http://hdl.handle.net/10889/7632.

MLA Handbook (7^th Edition):

Δροσοπούλου, Κωνσταντίνα. “Μηχανισμοί απόπτωσης και δράσης των μεταλλοπρωτεϊνασών στο τοίχωμα των έσω σπερματικών φλεβών σε ασθενείς με κιρσοκήλη.” 2014. Web. 05 Mar 2021.

Vancouver:

Δροσοπούλου �. Μηχανισμοί απόπτωσης και δράσης των μεταλλοπρωτεϊνασών στο τοίχωμα των έσω σπερματικών φλεβών σε ασθενείς με κιρσοκήλη. [Internet] [Masters thesis]. University of Patras; 2014. [cited 2021 Mar 05]. Available from: http://hdl.handle.net/10889/7632.

Council of Science Editors:

Δροσοπούλου �. Μηχανισμοί απόπτωσης και δράσης των μεταλλοπρωτεϊνασών στο τοίχωμα των έσω σπερματικών φλεβών σε ασθενείς με κιρσοκήλη. [Masters Thesis]. University of Patras; 2014. Available from: http://hdl.handle.net/10889/7632

Universidade Estadual de Campinas

26. Fontanelli, Beatriz Aparecida Fioruci, 1985-. Efeitos do consumo crônico de etanol sobre a atividade de MMP-2/MMP-9 e sobre o metabolismo do ácido retinóico nos lobos dorsais e laterais da próstata de ratos adultos = Effects of chronic ethanol consumption on the activity of MMP-2/MMP-9 and on retinoic acid metabolism in the dorsal and lateral prostate lobes of adult rats: Effects of chronic ethanol consumption on the activity of MMP-2/MMP-9 and on retinoic acid metabolism in the dorsal and lateral prostate lobes of adult rats.

Degree: 2014, Universidade Estadual de Campinas

URL: http://repositorio.unicamp.br/jspui/handle/REPOSIP/317953

► Abstract: Researchers have shown that chronic ethanol consumption alters the retinoic acid concentration, an active metabolite of vitamin A, in many organs including the prostate.… (more)

▼ Abstract: Researchers have shown that chronic ethanol consumption alters the retinoic acid concentration, an active metabolite of vitamin A, in many organs including the prostate. The retinoic acid is essential for the normal development of prostate and for maintaining its glandular homeostasis. Changes in concentration and metabolism of retinoic acid are related to lesion development in the prostate. Additionally, the activity of matrix metalloproteinases (MMPs), also relates to development of alterations in prostate. Thus, this study aimed to describe the effects of low and high doses of ethanol consumption, on the proteins involved in the synthesis and catabolism of retinoic acid (Article I), as well as on the enzymatic activity of MMPs (Article II) the dorsal and lateral lobes of the prostate. Twenty adult rats (~ 90 days old) of each variety, UChA and UChB, were divided into groups (n = 10 / group): UChA (low ethanol consumption, 0.2-2 g /kg / day), UChAC (rats not consumed ethanol); UChB (high ethanol consumption, > 2 g/ kg/ day), UChBC (rats not consumed ethanol). After the experimental period (~ 150 days old), the rats were euthanized by decapitation and dorsal and lateral lobes of the prostates were collected and dissected: (1) for evaluate the levels and location of the proteins ALDH1A1, ALDH1A2, ALDH1A3, CYP26A1, CYP26B1, CYP2E1, by western blot and immunohistochemistry, as well as, catabolic activity of CYP26A1, CYP26B1, CYP2E1 by biochemical assay and quantification by HPLC¿MS/MS; (2) and to evaluate the activity of MMP-2 and MMP-9, and the levels of tissue inhibitors of metalloproteinases (TIMP-1 / TIMP-2) using zymography and ELISA, respectively. In the UChA group, ALDH1A3 increased in dorsal prostate, while the proteins ALDH1A2 and ALDH1A1 decreased in the lateral prostate. In the UChB group, the proteins ALDH1A1, ALDH1A2 and ALDH1A3 increased in the dorsal prostate, while ALDH1A3 decreased in the lateral lobe. The concentration of retinoic acid increased, indicating a decrease in the CYP2E1 activity, and decreased when evaluated CYP26, indicating increased of CYP26 activity in the UChB dorsal prostate. Furthermore, the retinoic acid decreased when assessing the CYP total activity in the experimental groups, but only increased in the lateral prostate of UChA. The low ethanol consumption (UChA group) reduced the activities of MMP-2 and MMP-9 and the levels of TIMP-2 and TIMP-1 in the lateral prostate, while dorsal prostate the ethanol decreased the MMP-2 activity and the level of TIMP-1. On the other hand, in the UChB group, ethanol only decreased the activity of MMP-9 in the lateral prostate and did not alter the levels of TIMP-1 and TIMP-2. Our results indicate that ethanol modulates the synthesis and catabolism of retinoic acid in the rat prostate in a concentration-dependent manner. In addition, the chronic and low consumption of ethanol decreases the activity of metalloproteinases -2 and -9 in the lateral lobe prostate, showing that this organ is more susceptible to these changes than dorsal lobe… Advisors/Committee Members: UNIVERSIDADE ESTADUAL DE CAMPINAS (CRUESP), Felisbino, Sérgio Luis (advisor), Martinez, Francisco Eduardo (coadvisor), Universidade Estadual de Campinas. Instituto de Biologia (institution), Programa de Pós-Graduação em Biologia Celular e Estrutural (nameofprogram), Pereira, Sergio (committee member), Arena, Arielle Cristina (committee member), Schimming, Bruno César (committee member), Domeniconi, Raquel Fantin (committee member).

Subjects/Keywords: Álcool; Prostata; Tretinoína; Metaloproteinases da matriz; Ethanol; Prostate; Tretinoin; Matrix metalloproteinases

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Fontanelli, Beatriz Aparecida Fioruci, 1. (2014). Efeitos do consumo crônico de etanol sobre a atividade de MMP-2/MMP-9 e sobre o metabolismo do ácido retinóico nos lobos dorsais e laterais da próstata de ratos adultos = Effects of chronic ethanol consumption on the activity of MMP-2/MMP-9 and on retinoic acid metabolism in the dorsal and lateral prostate lobes of adult rats: Effects of chronic ethanol consumption on the activity of MMP-2/MMP-9 and on retinoic acid metabolism in the dorsal and lateral prostate lobes of adult rats. (Thesis). Universidade Estadual de Campinas. Retrieved from http://repositorio.unicamp.br/jspui/handle/REPOSIP/317953

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Fontanelli, Beatriz Aparecida Fioruci, 1985-. “Efeitos do consumo crônico de etanol sobre a atividade de MMP-2/MMP-9 e sobre o metabolismo do ácido retinóico nos lobos dorsais e laterais da próstata de ratos adultos = Effects of chronic ethanol consumption on the activity of MMP-2/MMP-9 and on retinoic acid metabolism in the dorsal and lateral prostate lobes of adult rats: Effects of chronic ethanol consumption on the activity of MMP-2/MMP-9 and on retinoic acid metabolism in the dorsal and lateral prostate lobes of adult rats.” 2014. Thesis, Universidade Estadual de Campinas. Accessed March 05, 2021. http://repositorio.unicamp.br/jspui/handle/REPOSIP/317953.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Fontanelli, Beatriz Aparecida Fioruci, 1985-. “Efeitos do consumo crônico de etanol sobre a atividade de MMP-2/MMP-9 e sobre o metabolismo do ácido retinóico nos lobos dorsais e laterais da próstata de ratos adultos = Effects of chronic ethanol consumption on the activity of MMP-2/MMP-9 and on retinoic acid metabolism in the dorsal and lateral prostate lobes of adult rats: Effects of chronic ethanol consumption on the activity of MMP-2/MMP-9 and on retinoic acid metabolism in the dorsal and lateral prostate lobes of adult rats.” 2014. Web. 05 Mar 2021.

Vancouver:

Fontanelli, Beatriz Aparecida Fioruci 1. Efeitos do consumo crônico de etanol sobre a atividade de MMP-2/MMP-9 e sobre o metabolismo do ácido retinóico nos lobos dorsais e laterais da próstata de ratos adultos = Effects of chronic ethanol consumption on the activity of MMP-2/MMP-9 and on retinoic acid metabolism in the dorsal and lateral prostate lobes of adult rats: Effects of chronic ethanol consumption on the activity of MMP-2/MMP-9 and on retinoic acid metabolism in the dorsal and lateral prostate lobes of adult rats. [Internet] [Thesis]. Universidade Estadual de Campinas; 2014. [cited 2021 Mar 05]. Available from: http://repositorio.unicamp.br/jspui/handle/REPOSIP/317953.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Fontanelli, Beatriz Aparecida Fioruci 1. Efeitos do consumo crônico de etanol sobre a atividade de MMP-2/MMP-9 e sobre o metabolismo do ácido retinóico nos lobos dorsais e laterais da próstata de ratos adultos = Effects of chronic ethanol consumption on the activity of MMP-2/MMP-9 and on retinoic acid metabolism in the dorsal and lateral prostate lobes of adult rats: Effects of chronic ethanol consumption on the activity of MMP-2/MMP-9 and on retinoic acid metabolism in the dorsal and lateral prostate lobes of adult rats. [Thesis]. Universidade Estadual de Campinas; 2014. Available from: http://repositorio.unicamp.br/jspui/handle/REPOSIP/317953

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Universidade Estadual de Campinas

27. Vidal, Cristina de Mattos Pimenta, 1984-. Estudo da atividade proteolítica da dentina humana sadia e cariada: Study of the proteolytic activity of human sound and carious dentin.

Degree: 2012, Universidade Estadual de Campinas

URL: http://repositorio.unicamp.br/jspui/handle/REPOSIP/289845

► Abstract: There are just over 15 years since the matrix metaloproteases (MMPs) were considered responsible for the degradation of dentin organic matrix in caries progression.… (more)

▼ Abstract: There are just over 15 years since the matrix metaloproteases (MMPs) were considered responsible for the degradation of dentin organic matrix in caries progression. From then on, only few studies were published based on this premise. More recently, it was showed that other proteases, like the cysteine-cathepsins (CTs), could also participate of such degradation process. The objective of this study was to evaluate and compare the abundance and proteolytic activity of different MMPs (MMP-2, -8 and -9) and CTs (B, K e L) in sound and carious dentin. Firstly, the abundance and localization of enzymes in sound and carious dentin was performed by conventional immunohistochemistry. In addition, MMP-2, -9 and CTs B and K abundance associated with evaluation of collagen structure in sound and carious dentin was done by immunofluorescence. Different dentin enzyme-extraction methods were also tested: 1) using guanidine-hydrochloride associated with EDTA (G-EDTA); 2) phosphoric acid (AF); 3) acetic acid and 4) guanidine-hydrochloride associated with acetic acid (G-AC). The presence of MMP-2, -8 e -9 and CTs B and K was evaluated by western blot. Proteolytic activity in dentin extracts was analyzed by zymography and spectrofluorometrically. The organic matrix solubilization in sound and carious dentin was evaluated by hydroxyproline assay (HYP). Immunohistochemistry showed the presence of MMP-2, -8 and -9 and CTs B, K and L, especially in deep dentin and predentin, showing more abundance in caries. The same results were observed in immunofluorescence, which also showed that the collagen structure was modified in carious dentin. The presence of MMP-2, -8 and -9 and CTs B and K was showed in western blot in all extraction protocols evaluated, with more abundance in carious when compared to sound dentin. Gelatinolytic activity corresponding to MMP-2 was showed in zymography, but it was different according to the extraction protocol. Proteolytic activity for MMPs and CTs was observed spectrofluorometrically in sound dentin and some variation was observed according to the extraction protocol. Dentin organic matrix solubilization was confirmed by HYP, with higher HYP released in AC extraction protocol. When comparing sound and carious dentin, organic matrix degradation was higher in caries. It can be concluded that, besides MMP-2, -8 and -9, the CTs B, K and L are also present in sound and carious dentin, with higher abundance in caries. However, the presence and activity may vary according to the extraction method used. The results indicate that both MMPs and CTs may act in concert in dentin organic matrix degradation in caries progression and also support the physiopathology theory for caries in which a host-derived proteolytic mechanism would be responsible for dentin degradation Advisors/Committee Members: UNIVERSIDADE ESTADUAL DE CAMPINAS (CRUESP), Carrilho, Marcela Rocha de Oliveira (advisor), Universidade Estadual de Campinas. Faculdade de Odontologia de Piracicaba (institution), Programa de Pós-Graduação em Materiais Dentários (nameofprogram), Francci, Carlos Eduardo (committee member), Tersariol, Ivarne Luis Santos (committee member), Giannini, Marcelo (committee member), Alonso, Roberta Caroline Bruschi (committee member).

Subjects/Keywords: Metaloproteinases da matriz; Enzimas; Cárie dentária; Matrix metalloproteinases; Enzymes; Dental caries

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APA (6^th Edition):

Vidal, Cristina de Mattos Pimenta, 1. (2012). Estudo da atividade proteolítica da dentina humana sadia e cariada: Study of the proteolytic activity of human sound and carious dentin. (Thesis). Universidade Estadual de Campinas. Retrieved from http://repositorio.unicamp.br/jspui/handle/REPOSIP/289845

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Vidal, Cristina de Mattos Pimenta, 1984-. “Estudo da atividade proteolítica da dentina humana sadia e cariada: Study of the proteolytic activity of human sound and carious dentin.” 2012. Thesis, Universidade Estadual de Campinas. Accessed March 05, 2021. http://repositorio.unicamp.br/jspui/handle/REPOSIP/289845.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Vidal, Cristina de Mattos Pimenta, 1984-. “Estudo da atividade proteolítica da dentina humana sadia e cariada: Study of the proteolytic activity of human sound and carious dentin.” 2012. Web. 05 Mar 2021.

Vancouver:

Vidal, Cristina de Mattos Pimenta 1. Estudo da atividade proteolítica da dentina humana sadia e cariada: Study of the proteolytic activity of human sound and carious dentin. [Internet] [Thesis]. Universidade Estadual de Campinas; 2012. [cited 2021 Mar 05]. Available from: http://repositorio.unicamp.br/jspui/handle/REPOSIP/289845.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Vidal, Cristina de Mattos Pimenta 1. Estudo da atividade proteolítica da dentina humana sadia e cariada: Study of the proteolytic activity of human sound and carious dentin. [Thesis]. Universidade Estadual de Campinas; 2012. Available from: http://repositorio.unicamp.br/jspui/handle/REPOSIP/289845

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

McMaster University

28. Siwakoti, Anuja. CONSEQUENCE OF MMP-9 DEFICIENCY ON INTRAOCULAR PRESSURE REGULATION AND RETINAL GANGLION CELL SURVIVAL.

Degree: MSc, 2014, McMaster University

URL: http://hdl.handle.net/11375/16035

►

Matrix metalloproteinases (MMPs) are known to be the mediators of extracellular matrix remodeling. Increased levels of matrix metalloproteinases, particularly MMP-9, have been found in the… (more)

▼

Matrix metalloproteinases (MMPs) are known to be the mediators of extracellular matrix remodeling. Increased levels of matrix metalloproteinases, particularly MMP-9, have been found in the aqueous humor of patients with glaucoma. However the exact role of MMP-9 in glaucomatous changes is not understood. Previous results from the West-Mays’ lab indicated that MMP-9 deficient (knockout - KO) mice exhibit elevated IOP, in the absence of distinct morphological changes in the anterior chamber. In the current thesis, I investigated whether the elevated IOP in MMP-9KO mice leads to RGC death. Wild type and KO littermates at different age groups: 2-3 months, 3-4 months, 6-8 and 9-12 months were studied. IOP was measured using TonoLab rebound tonometer. My results demonstrated that IOP was significantly increased in MMP-9KO mice compared to control littermates at all ages examined. To investigate if the elevated IOP was due to a difference in central corneal thickness (CCT), CCT measurements were made between WT and KO mice using ultrasound pachymeter. There was no difference in CCT demonstrating that the elevated IOP observed in MMP-9KO mice was not related to changes in corneal thickness. To determine whether the elevated IOP led to RGC death, the animals were sacrificed, eyes were enucleated and retinas (n=4) from both WT and KO animals were dissected and stained with Brn-3a antibody. Additional eyes were harvested from both WT and KO mice for histological and immunofluorescence studies. I found no observable difference in Brn3a+ RGC count between MMP9-WT and KO mice. Furthermore, no difference in retinal morphology, glial reactivity and laminin expression between WT and KO mice was observed. In the future it will be important to investigate whether elevated IOP in the MMP-9KO mice leads to optic nerve axonal loss and further investigate the possibility that the MMP-9KO retina is neuroprotected.

Thesis

Master of Science (MSc)

Advisors/Committee Members: West-Mays, Judith, Medical Sciences.

Subjects/Keywords: glaucoma; matrix metalloproteinases; MMP-9; IOP; RGC; retina

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Siwakoti, A. (2014). CONSEQUENCE OF MMP-9 DEFICIENCY ON INTRAOCULAR PRESSURE REGULATION AND RETINAL GANGLION CELL SURVIVAL. (Masters Thesis). McMaster University. Retrieved from http://hdl.handle.net/11375/16035

Chicago Manual of Style (16^th Edition):

Siwakoti, Anuja. “CONSEQUENCE OF MMP-9 DEFICIENCY ON INTRAOCULAR PRESSURE REGULATION AND RETINAL GANGLION CELL SURVIVAL.” 2014. Masters Thesis, McMaster University. Accessed March 05, 2021. http://hdl.handle.net/11375/16035.

MLA Handbook (7^th Edition):

Siwakoti, Anuja. “CONSEQUENCE OF MMP-9 DEFICIENCY ON INTRAOCULAR PRESSURE REGULATION AND RETINAL GANGLION CELL SURVIVAL.” 2014. Web. 05 Mar 2021.

Vancouver:

Siwakoti A. CONSEQUENCE OF MMP-9 DEFICIENCY ON INTRAOCULAR PRESSURE REGULATION AND RETINAL GANGLION CELL SURVIVAL. [Internet] [Masters thesis]. McMaster University; 2014. [cited 2021 Mar 05]. Available from: http://hdl.handle.net/11375/16035.

Council of Science Editors:

Siwakoti A. CONSEQUENCE OF MMP-9 DEFICIENCY ON INTRAOCULAR PRESSURE REGULATION AND RETINAL GANGLION CELL SURVIVAL. [Masters Thesis]. McMaster University; 2014. Available from: http://hdl.handle.net/11375/16035

University of Toronto

29. Burgess, Allysa Jane Laura. Topical Molecular Beacons for In Vivo Image-guided Resection of Oral Carcinoma.

Degree: 2014, University of Toronto

URL: http://hdl.handle.net/1807/67951

►

Oral carcinoma has become a major health problem, with nearly 300,000 people diagnosed worldwide annually. The 5-year survival rate is as low as 30%, mainly… (more)

Subjects/Keywords: matrix metalloproteinases; molecular beacons; molecular imaging; oral carcinoma; 0786

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APA (6^th Edition):

Burgess, A. J. L. (2014). Topical Molecular Beacons for In Vivo Image-guided Resection of Oral Carcinoma. (Masters Thesis). University of Toronto. Retrieved from http://hdl.handle.net/1807/67951

Chicago Manual of Style (16^th Edition):

Burgess, Allysa Jane Laura. “Topical Molecular Beacons for In Vivo Image-guided Resection of Oral Carcinoma.” 2014. Masters Thesis, University of Toronto. Accessed March 05, 2021. http://hdl.handle.net/1807/67951.

MLA Handbook (7^th Edition):

Burgess, Allysa Jane Laura. “Topical Molecular Beacons for In Vivo Image-guided Resection of Oral Carcinoma.” 2014. Web. 05 Mar 2021.

Vancouver:

Burgess AJL. Topical Molecular Beacons for In Vivo Image-guided Resection of Oral Carcinoma. [Internet] [Masters thesis]. University of Toronto; 2014. [cited 2021 Mar 05]. Available from: http://hdl.handle.net/1807/67951.

Council of Science Editors:

Burgess AJL. Topical Molecular Beacons for In Vivo Image-guided Resection of Oral Carcinoma. [Masters Thesis]. University of Toronto; 2014. Available from: http://hdl.handle.net/1807/67951

University of Toronto

30. Nguyen, Tina Tu-Thu Ngoc. Matrix Metalloproteinase Expression and Regulation During Pregnancy, Term Labour, and Postpartum.

Degree: 2015, University of Toronto

URL: http://hdl.handle.net/1807/70507

►

It is widely accepted that pregnancy, spontaneous term labour (TL), and postpartum (PP) involution is associated with changes in the cellular and extracellular composition of… (more)

Subjects/Keywords: matrix metalloproteinases; myometrium; postpartum; pregnancy; term labour; 0719

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Nguyen, T. T. N. (2015). Matrix Metalloproteinase Expression and Regulation During Pregnancy, Term Labour, and Postpartum. (Masters Thesis). University of Toronto. Retrieved from http://hdl.handle.net/1807/70507

Chicago Manual of Style (16^th Edition):

Nguyen, Tina Tu-Thu Ngoc. “Matrix Metalloproteinase Expression and Regulation During Pregnancy, Term Labour, and Postpartum.” 2015. Masters Thesis, University of Toronto. Accessed March 05, 2021. http://hdl.handle.net/1807/70507.

MLA Handbook (7^th Edition):

Nguyen, Tina Tu-Thu Ngoc. “Matrix Metalloproteinase Expression and Regulation During Pregnancy, Term Labour, and Postpartum.” 2015. Web. 05 Mar 2021.

Vancouver:

Nguyen TTN. Matrix Metalloproteinase Expression and Regulation During Pregnancy, Term Labour, and Postpartum. [Internet] [Masters thesis]. University of Toronto; 2015. [cited 2021 Mar 05]. Available from: http://hdl.handle.net/1807/70507.

Council of Science Editors:

Nguyen TTN. Matrix Metalloproteinase Expression and Regulation During Pregnancy, Term Labour, and Postpartum. [Masters Thesis]. University of Toronto; 2015. Available from: http://hdl.handle.net/1807/70507

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Open Access Theses and Dissertations