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1.
Patricia Regina de Souza.
Avaliação de técnicas cromatográficas acopladas a espectrometria de massas para análise de morfolina em manga.
Degree: 2016, University of São Paulo
URL: http://www.teses.usp.br/teses/disponiveis/75/75135/tde-18052016-143752/ 
► A constante preocupação com o aumento do uso de agrotóxicos nas lavouras e os riscos gerados pelos resíduos destes compostos fazem com que os órgãos…
(more)
▼ A constante preocupação com o aumento do uso de agrotóxicos nas lavouras e os riscos gerados pelos resíduos destes compostos fazem com que os órgãos responsáveis pela fiscalização de alimentos no Brasil controlem a presença dessas substâncias nos produtos que chegam à mesa do consumidor. Atualmente, um dos grandes problemas na produção de alimentos é a utilização de substâncias proibidas em lavouras, muitas das quais não possuem estudos nem limites máximos de resíduos (LMR) estabelecidos, assim como a utilização de substâncias já registradas, mas em quantidades ou métodos de manejo incorretos. Ambos os casos podem resultar em sérios problemas à saúde humana. O objetivo deste estudo foi a avaliação da determinação de morfolina em amostras de manga utilizando técnicas como a Extração em Fase Sólida e a Cromatografia Gasosa acoplada à Espectrometria de Massas (SPE-GC-MS), assim como a Microextração em Sorvente Empacotado e Cromatografia Gasosa
acoplada à Espectrometria de Massas (MEPS-GC-MS). Um segundo objetivo deste estudo consistiu em desenvolver, validar e avaliar uma metodologia analítica capaz de identificar quantitativamente a morfolina em amostras de manga por Cromatografia Líquida de Ultra Eficiência acoplada a Espectrometria de Massas em tandem (UHPLC-MS/MS). Para análise por GC-MS fez-se necessária a etapa de derivatização do analito, de forma que o mesmo aumentasse sua volatilidade e diminuísse a polaridade. A comparação entre as técnicas SPE e MEPS não foi possível devido ao efeito de matriz causado pela contaminação do liner e da coluna cromatográfica. Já a metodologia validada por UHPLC-MS/MS seguiu os critérios exigidos pelo Manual de Garantia da Qualidade Analítica, do Ministério da Agricultura Pecuária e Abastecimento (MAPA). O método foi aplicado em mangas de diferentes variedades obtidas no comércio local. Não foram encontrados resíduos de morfolina em nenhuma das amostras investigadas, de acordo com a
metodologia proposta. Os resultados apresentados neste trabalho estabelecem metodologias eficientes, rápidas e de baixo custo na determinação de morfolina em amostras de manga.
The recurrent increasing of the use of pesticides on crops and the consequent risks due the exposure to chemical residues have urged the food regulation agencies to control the levels of these substances in products that reach consumer\'s table. A major problem nowadays for the production of food is the use of banned substances in crops and the extrapolation of the limit dosages of substances, which may result in serious problems to human health. Furthermore, many of these substances commonly used in crops still lack substantial information about the maximum residue levels (MRLs). The aim of this study was the evaluation of morpholine levels in mango samples using distinct techniques such as Solid Phase Extraction followed by Gas Chromatography coupled to Mass Spectrometry (SPE-GC-MS) and Microextraction
by Packed Sorbent followed by Gas Chromatography Mass Spectrometry (MEPS-GC-MS). Another main goal of this work is…
Advisors/Committee Members: Fernando Mauro Lanças, Silvia Helena Govoni Brondi.
Subjects/Keywords: GC-MS; manga; Morfolina; UHPLC-MS/MS; GC-MS; mango; Morpholine; UHPLC-MS/MS
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APA (6th Edition):
Souza, P. R. d. (2016). Avaliação de técnicas cromatográficas acopladas a espectrometria de massas para análise de morfolina em manga. (Masters Thesis). University of São Paulo. Retrieved from http://www.teses.usp.br/teses/disponiveis/75/75135/tde-18052016-143752/
Chicago Manual of Style (16th Edition):
Souza, Patricia Regina de. “Avaliação de técnicas cromatográficas acopladas a espectrometria de massas para análise de morfolina em manga.” 2016. Masters Thesis, University of São Paulo. Accessed April 18, 2021.
http://www.teses.usp.br/teses/disponiveis/75/75135/tde-18052016-143752/.
MLA Handbook (7th Edition):
Souza, Patricia Regina de. “Avaliação de técnicas cromatográficas acopladas a espectrometria de massas para análise de morfolina em manga.” 2016. Web. 18 Apr 2021.
Vancouver:
Souza PRd. Avaliação de técnicas cromatográficas acopladas a espectrometria de massas para análise de morfolina em manga. [Internet] [Masters thesis]. University of São Paulo; 2016. [cited 2021 Apr 18].
Available from: http://www.teses.usp.br/teses/disponiveis/75/75135/tde-18052016-143752/.
Council of Science Editors:
Souza PRd. Avaliação de técnicas cromatográficas acopladas a espectrometria de massas para análise de morfolina em manga. [Masters Thesis]. University of São Paulo; 2016. Available from: http://www.teses.usp.br/teses/disponiveis/75/75135/tde-18052016-143752/
Universidade Federal de Santa Maria
2.
Filipe Fagan Donato.
RESÍDUOS DE AGROTÓXICOS EM ÁGUA POTÁVEL USANDO SPE E DETERMINAÇÃO RÁPIDA POR LC-MS/MS E GC-MS/MS.
Degree: 2012, Universidade Federal de Santa Maria
URL: http://coralx.ufsm.br/tede/tde_busca/arquivo.php?codArquivo=5162
► O uso de agrotóxicos sempre esteve associado à efetividade no controle de pragas ou plantas invasoras para aumentar a produção de alimentos. No entanto, o…
(more)
▼ O uso de agrotóxicos sempre esteve associado à efetividade no controle de pragas ou plantas invasoras para aumentar a produção de alimentos. No entanto, o uso indiscriminado dessas substâncias vem causando a degradação dos recursos hídricos. No Brasil, o Ministério da Saúde através da Portaria 2914 define diversos parâmetros de potabilidade, entre eles, os limites máximos permitidos para alguns agrotóxicos. No presente trabalho foi desenvolvido e validado um método para a determinação de resíduos de 70 agrotóxicos em água potável, utilizando Extração em Fase Sólida (SPE) para o preparo de amostra e determinação por Cromatografia Gasosa e Líquida, acopladas à Espectrometria de Massas sequencial, com analisador triplo quadrupolo (GC-(TQ)MS/MS e LC- (TQ)MS/MS). Foram avaliados diferentes volumes de amostra, sorventes e solventes de eluição. Os melhores resultados foram obtidos com 100 mL de amostra acidificada em pH 2,5, cartucho SPE Oasis HLB 60 mg/3 mL e diclorometano/metanol como eluente. As curvas analíticas apresentaram linearidade entre 10 e 250 Sg L-1, com valores de r2 maiores que 0,99 para todos os compostos. Os valores de LOQ do método foram 0,02 Sg L-1 para aldrin, dieldrin e clordano e de 0,5 Sg L-1 para os demais compostos. Para avaliação da exatidão as amostras branco foram fortificadas em 0,5, 1,5 e 4,0 Sg L-1 e um nível extra em 0,02 Sg L-1 para os compostos aldrin, dieldrin e clordano. O método apresentou boa precisão, com valores de RSD inferiores a 20% e boa exatidão, com recuperações entre 70 e 120%. Apenas os compostos metamidofós, aldicarbe, benfuracarbe, terbufós, benomil e tiofanato metílico não foram recuperados de forma adequada. O efeito matriz foi avaliado, mostrando-se superior a 10% para a maioria dos compostos. A fim de compensar este efeito, utilizou-se curvas analíticas preparadas no extrato branco da matriz. O método validado foi aplicado em 12 amostras de água potável de diferentes características (rio, vertente, poço e água tratada), sendo que apenas uma das amostras de água de rio apresentou resíduos de lambda-cialotrina. Os resultados indicam que o método proposto é adequado à análise de resíduos de agrotóxicos em água potável, visto que todos os parâmetros de validação atenderam os limites e parâmetros sugeridos para validação de métodos cromatográficos.
The use of pesticides always has been associated with the effective control of pests or invasive weeds to ensure an increase in the food production. However, the indiscriminate use of these substances has caused the degradation of water resources. In Brazil, the Ministry of Health through Ordinance 2914 defines several parameters of potability, among them, the maximum limits allowed for same pesticides. In this work it was developed and validated a method for the determination of residues of 70 pesticides in drinking water using (SPE) for sample preparation and determination by Gas and Liquid Chromatography coupled to tandem mass spectrometry, triple quadrupole analyzer (GC-(TQ)MS/MS and LC- (TQ)MS/MS). It was evaluated…
Advisors/Committee Members: Renato Zanella, Osmar Damian Prestes, Manoel Leonardo Martins.
Subjects/Keywords: GC-MS/MS; LC-MS/MS; água; SPE; agrotóxicos; LC-MS/MS; GC-MS/MS; SPE; pesticides; water; QUIMICA
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APA (6th Edition):
Donato, F. F. (2012). RESÍDUOS DE AGROTÓXICOS EM ÁGUA POTÁVEL USANDO SPE E DETERMINAÇÃO RÁPIDA POR LC-MS/MS E GC-MS/MS. (Thesis). Universidade Federal de Santa Maria. Retrieved from http://coralx.ufsm.br/tede/tde_busca/arquivo.php?codArquivo=5162
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Donato, Filipe Fagan. “RESÍDUOS DE AGROTÓXICOS EM ÁGUA POTÁVEL USANDO SPE E DETERMINAÇÃO RÁPIDA POR LC-MS/MS E GC-MS/MS.” 2012. Thesis, Universidade Federal de Santa Maria. Accessed April 18, 2021.
http://coralx.ufsm.br/tede/tde_busca/arquivo.php?codArquivo=5162.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Donato, Filipe Fagan. “RESÍDUOS DE AGROTÓXICOS EM ÁGUA POTÁVEL USANDO SPE E DETERMINAÇÃO RÁPIDA POR LC-MS/MS E GC-MS/MS.” 2012. Web. 18 Apr 2021.
Vancouver:
Donato FF. RESÍDUOS DE AGROTÓXICOS EM ÁGUA POTÁVEL USANDO SPE E DETERMINAÇÃO RÁPIDA POR LC-MS/MS E GC-MS/MS. [Internet] [Thesis]. Universidade Federal de Santa Maria; 2012. [cited 2021 Apr 18].
Available from: http://coralx.ufsm.br/tede/tde_busca/arquivo.php?codArquivo=5162.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Donato FF. RESÍDUOS DE AGROTÓXICOS EM ÁGUA POTÁVEL USANDO SPE E DETERMINAÇÃO RÁPIDA POR LC-MS/MS E GC-MS/MS. [Thesis]. Universidade Federal de Santa Maria; 2012. Available from: http://coralx.ufsm.br/tede/tde_busca/arquivo.php?codArquivo=5162
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Vilnius University
3.
Tamošiūnas, Vytautas.
Skysčių chromatografija–tandeminė masių
spektrometrija antibiotikų nustatymui maisto
produktuose.
Degree: Dissertation, Chemistry, 2009, Vilnius University
URL: http://vddb.laba.lt/obj/LT-eLABa-0001:E.02~2009~D_20090526_111309-82462
;
► Efektyviosios (ESCh) ir/arba ultraefektyviosios (UESCh) skysčių chromatografijos-tandeminės masių spektrometrijos (MS/MS) metodai ištirti kai kurių antibiotikų (chloramfenikolio, sulfonamidų, nitroimidazolų ir jų metabolitų) nustatymui maisto produktuose. Ištirta…
(more)
▼ Efektyviosios (ESCh) ir/arba
ultraefektyviosios (UESCh) skysčių chromatografijos-tandeminės
masių spektrometrijos (MS/MS) metodai ištirti kai kurių antibiotikų
(chloramfenikolio, sulfonamidų, nitroimidazolų ir jų metabolitų)
nustatymui maisto produktuose. Ištirta įvairių faktorių įtaka
antibiotikų atskyrimui ESCh bei UESCh metodais ir MS/MS
detektavimui. Antibiotikų išskyrimui iš pieno, kiaušinių ir medaus
matricų ir mėginių valymui ištirtos ir pritaikytos naujos,
efektyvios ir greitos skysčių-skysčių bei kietafazės ekstrakcijos
procedūros. Nuodugniai įvertinus metodų analizines charakteristikas
nustatyta, kad metodai pasižymi geru tikslumu, atrankumu, jautriu
bei rezultatų glaudumu ir puikiai tenkina ES reikalavimus, keliamus
veterinarinių vaistų likučių nustatymui maisto produktuose. Nauji
metodai įteisinti Nacionaliniame maisto ir veterinarijos rizikos
vertinimo institute ir sėkmingai naudojami veterinarinių vaistų
likučių monitoringui lietuviškuose maisto
produktuose.
High–performance liquid chromatography
(HPLC) and/or ultra-performance liquid chromatography (UPLC)
techniques coupled with tandem mass spectrometry (MS/MS) were
developed for the determination of common antibiotics
(chloramphenicol, sulfonamides and nitroimidazoles together with
their metabolites) in food products. HPLC/UPLC and MS/MS conditions
were optimized for the separation and detection of the drugs by
varying common experimental parameters. Effective and reliable
sample extraction and clean-up procedures based on liquid-liquid
extraction and/or solid-phase extraction techniques were developed
for milk, honey and egg matrices. Proposed methods were validated
according to EU criteria applying the alternative validation
approach and the obtained results showed that methods are
selective, accurate, reproducible and sensitive enough. Finally,
the developed methods were successfully applied for the monitoring
of Lithuanian food products.
Advisors/Committee Members: Tautkus, Stasys (Doctoral dissertation committee chair), Brukštus, Algirdas (Doctoral dissertation committee member), Jurkonienė, Sigita (Doctoral dissertation committee member), Misiūnas, Audrius (Doctoral dissertation committee member), Norkus, Eugenijus (Doctoral dissertation committee member), Denafas, Gintaras (Doctoral dissertation opponent), Vičkačkaitė, Vida (Doctoral dissertation opponent), Padarauskas, Audrius (Doctoral dissertation supervisor).
Subjects/Keywords: Chloramfenikolis; Sulfonamidai;
5-nitroimidazolai; ESCh-MS/MS; UESCh-MS/MS;
Chloramphenicol; Sulphonamides;
5-nitroimidazoles; HPLC-MS/MS; UPLC-MS/MS
Record Details
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Record Details
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❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Tamošiūnas, V. (2009). Skysčių chromatografija–tandeminė masių
spektrometrija antibiotikų nustatymui maisto
produktuose. (Doctoral Dissertation). Vilnius University. Retrieved from http://vddb.laba.lt/obj/LT-eLABa-0001:E.02~2009~D_20090526_111309-82462 ;
Chicago Manual of Style (16th Edition):
Tamošiūnas, Vytautas. “Skysčių chromatografija–tandeminė masių
spektrometrija antibiotikų nustatymui maisto
produktuose.” 2009. Doctoral Dissertation, Vilnius University. Accessed April 18, 2021.
http://vddb.laba.lt/obj/LT-eLABa-0001:E.02~2009~D_20090526_111309-82462 ;.
MLA Handbook (7th Edition):
Tamošiūnas, Vytautas. “Skysčių chromatografija–tandeminė masių
spektrometrija antibiotikų nustatymui maisto
produktuose.” 2009. Web. 18 Apr 2021.
Vancouver:
Tamošiūnas V. Skysčių chromatografija–tandeminė masių
spektrometrija antibiotikų nustatymui maisto
produktuose. [Internet] [Doctoral dissertation]. Vilnius University; 2009. [cited 2021 Apr 18].
Available from: http://vddb.laba.lt/obj/LT-eLABa-0001:E.02~2009~D_20090526_111309-82462 ;.
Council of Science Editors:
Tamošiūnas V. Skysčių chromatografija–tandeminė masių
spektrometrija antibiotikų nustatymui maisto
produktuose. [Doctoral Dissertation]. Vilnius University; 2009. Available from: http://vddb.laba.lt/obj/LT-eLABa-0001:E.02~2009~D_20090526_111309-82462 ;
Vilnius University
4.
Tamošiūnas, Vytautas.
Liquid chromatography-tandem mass spectrometry for
the determination of antibiotics in food products.
Degree: PhD, Chemistry, 2009, Vilnius University
URL: http://vddb.laba.lt/obj/LT-eLABa-0001:E.02~2009~D_20090526_111320-24950
;
► High–performance liquid chromatography (HPLC) and/or ultra-performance liquid chromatography (UPLC) techniques coupled with tandem mass spectrometry (MS/MS) were developed for the determination of common antibiotics (chloramphenicol,…
(more)
▼ High–performance liquid chromatography
(HPLC) and/or ultra-performance liquid chromatography (UPLC)
techniques coupled with tandem mass spectrometry (MS/MS) were
developed for the determination of common antibiotics
(chloramphenicol, sulfonamides and nitroimidazoles together with
their metabolites) in food products. HPLC/UPLC and MS/MS conditions
were optimized for the separation and detection of the drugs by
varying common experimental parameters. Effective and reliable
sample extraction and clean-up procedures based on liquid-liquid
extraction and/or solid-phase extraction techniques were developed
for milk, honey and egg matrices. Proposed methods were validated
according to EU criteria applying the alternative validation
approach and the obtained results showed that methods are
selective, accurate, reproducible and sensitive enough. Finally,
the developed methods were successfully applied for the monitoring
of Lithuanian food products.
Efektyviosios (ESCh) ir/arba
ultraefektyviosios (UESCh) skysčių chromatografijos-tandeminės
masių spektrometrijos (MS/MS) metodai ištirti kai kurių antibiotikų
(chloramfenikolio, sulfonamidų, nitroimidazolų ir jų metabolitų)
nustatymui maisto produktuose. Ištirta įvairių faktorių įtaka
antibiotikų atskyrimui ESCh bei UESCh metodais ir MS/MS
detektavimui. Antibiotikų išskyrimui iš pieno, kiaušinių ir medaus
matricų ir mėginių valymui ištirtos ir pritaikytos naujos,
efektyvios ir greitos skysčių-skysčių bei kietafazės ekstrakcijos
procedūros. Nuodugniai įvertinus metodų analizines charakteristikas
nustatyta, kad metodai pasižymi geru tikslumu, atrankumu, jautriu
bei rezultatų glaudumu ir puikiai tenkina ES reikalavimus, keliamus
veterinarinių vaistų likučių nustatymui maisto produktuose. Nauji
metodai įteisinti Nacionaliniame maisto ir veterinarijos rizikos
vertinimo institute ir sėkmingai naudojami veterinarinių vaistų
likučių monitoringui lietuviškuose maisto
produktuose.
Advisors/Committee Members: Tautkus, Stasys (Doctoral dissertation committee chair), Brukštus, Algirdas (Doctoral dissertation committee member), Jurkonienė, Sigita (Doctoral dissertation committee member), Misiūnas, Audrius (Doctoral dissertation committee member), Norkus, Eugenijus (Doctoral dissertation committee member), Denafas, Gintaras (Doctoral dissertation opponent), Vičkačkaitė, Vida (Doctoral dissertation opponent), Padarauskas, Audrius (Doctoral dissertation supervisor).
Subjects/Keywords: Chloramphenicol; Sulfonamides;
5-nitroimidazoles; HPLC-MS/MS; UPLC-MS/MS;
Chlooramfenikolis; Sulfonamidai;
5-nitroimidazolai; ESCh-MS/MS; UESCh-MS/MS
Record Details
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Share »
Record Details
Similar Records
Cite
« Share
❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Tamošiūnas, V. (2009). Liquid chromatography-tandem mass spectrometry for
the determination of antibiotics in food products. (Doctoral Dissertation). Vilnius University. Retrieved from http://vddb.laba.lt/obj/LT-eLABa-0001:E.02~2009~D_20090526_111320-24950 ;
Chicago Manual of Style (16th Edition):
Tamošiūnas, Vytautas. “Liquid chromatography-tandem mass spectrometry for
the determination of antibiotics in food products.” 2009. Doctoral Dissertation, Vilnius University. Accessed April 18, 2021.
http://vddb.laba.lt/obj/LT-eLABa-0001:E.02~2009~D_20090526_111320-24950 ;.
MLA Handbook (7th Edition):
Tamošiūnas, Vytautas. “Liquid chromatography-tandem mass spectrometry for
the determination of antibiotics in food products.” 2009. Web. 18 Apr 2021.
Vancouver:
Tamošiūnas V. Liquid chromatography-tandem mass spectrometry for
the determination of antibiotics in food products. [Internet] [Doctoral dissertation]. Vilnius University; 2009. [cited 2021 Apr 18].
Available from: http://vddb.laba.lt/obj/LT-eLABa-0001:E.02~2009~D_20090526_111320-24950 ;.
Council of Science Editors:
Tamošiūnas V. Liquid chromatography-tandem mass spectrometry for
the determination of antibiotics in food products. [Doctoral Dissertation]. Vilnius University; 2009. Available from: http://vddb.laba.lt/obj/LT-eLABa-0001:E.02~2009~D_20090526_111320-24950 ;
Brno University of Technology
5.
Müller, Lukáš.
Analýza proteomu piva pomocí hmotnostní spektrometrie: Beer proteome analysis by mass spectrometry.
Degree: 2018, Brno University of Technology
URL: http://hdl.handle.net/11012/13465
► The aim of presented diploma thesis was to characterize recent knowledge in the field of beer proteomics. The main part of this work was focused…
(more)
▼ The aim of presented diploma thesis was to characterize recent knowledge in the field of beer proteomics. The main part of this work was focused on modern instrumental methods of protein analysis, especially on protein identification by mass spectrometry. In experimental part proteins from selected beer samples were isolated, purified and separated by 2-D electrophoresis. The identification was performed by MALDI
MS/MS and LC-
MS/MS. Identified proteins were divided into 6 groups - serpines and protein Z, trypsine/-amylase inhibitors, yeast proteins, LTP protein, hordeins and other proteins. Proteomic analysis provided identification of proteins important for final analytical and sensory characteristics of the beer - for final beer quality and taste
Advisors/Committee Members: Zdráhal, Zbyněk (advisor), Havliš,, Jan (referee).
Subjects/Keywords: separace proteinů; proteiny piva; LC-MS/MS; MALDI-MS/MS; protein separation; beer proteins; LC/MS/MS; MALDI-MS/MS
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APA ·
Chicago ·
MLA ·
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CSE |
Export
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Manager
APA (6th Edition):
Müller, L. (2018). Analýza proteomu piva pomocí hmotnostní spektrometrie: Beer proteome analysis by mass spectrometry. (Thesis). Brno University of Technology. Retrieved from http://hdl.handle.net/11012/13465
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Müller, Lukáš. “Analýza proteomu piva pomocí hmotnostní spektrometrie: Beer proteome analysis by mass spectrometry.” 2018. Thesis, Brno University of Technology. Accessed April 18, 2021.
http://hdl.handle.net/11012/13465.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Müller, Lukáš. “Analýza proteomu piva pomocí hmotnostní spektrometrie: Beer proteome analysis by mass spectrometry.” 2018. Web. 18 Apr 2021.
Vancouver:
Müller L. Analýza proteomu piva pomocí hmotnostní spektrometrie: Beer proteome analysis by mass spectrometry. [Internet] [Thesis]. Brno University of Technology; 2018. [cited 2021 Apr 18].
Available from: http://hdl.handle.net/11012/13465.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Müller L. Analýza proteomu piva pomocí hmotnostní spektrometrie: Beer proteome analysis by mass spectrometry. [Thesis]. Brno University of Technology; 2018. Available from: http://hdl.handle.net/11012/13465
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
6.
Harakat, Dominique.
Etudes mécanistiques par ESI-MS de réactions palladocatalysées : Mechanism studies of palladocatalyzed reactions by ESI-MS.
Degree: Docteur es, Chimie organique, 2011, Reims
URL: http://www.theses.fr/2011REIMS010
► Ce mémoire décrit l'utilisation de la spectrométrie de masse en mode électrospray pour l'étude mécanistique de réactions catalytiques.Dans le premier chapitre, le mécanisme de la…
(more)
▼ Ce mémoire décrit l'utilisation de la spectrométrie de masse en mode électrospray pour l'étude mécanistique de réactions catalytiques.Dans le premier chapitre, le mécanisme de la réaction de Wacker est étudié ; l'implication d'espèces dinucléaires en palladium, dans le cas de l'utilisation de la benzoquinone comme oxydant, est mise en évidence. La réoxydation du palladium avant la décomplexation du produit a également été établie.Le deuxième chapitre est consacré à l'étude mécanistique de l'oxydation de l'allylphénole en milieu aqueux. La détection d'un intermédiaire epoxyde confirme un mécanisme en cascade : isomérisation, epoxydation et solvolyse.L'étude du mécanisme de réactions de type Heck déshydrogénant représente le dernier chapitre, quatre intermédiaires clefs du cycle catalytique proposé, ont été détectés.
This thesis describes the use of electrospray spectrometry for mechanistic studies of catalytic reactions. The mechanism of the Wacker reaction has been studied in the first chapter ; the implication of dinuclear palladium species, using benzoquinone as oxidant, has been demonstrated. The reoxidation of the palladium before the decoordination of the product has also been established. The second chapter focuses on the mechanism study of the oxidation of allylphenol in aqueous media. The detection of an epoxyde as intermediate corroborates the proposed mechanism : isomerisation, epoxydation solvolysis. The mechanism of a Dehydrogenative Heck reaction is described in the last chapter. Four key intermediates of the catalytic cycle have been identified.
Advisors/Committee Members: Le Bras, Jean (thesis director).
Subjects/Keywords: ESI-MS
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APA (6th Edition):
Harakat, D. (2011). Etudes mécanistiques par ESI-MS de réactions palladocatalysées : Mechanism studies of palladocatalyzed reactions by ESI-MS. (Doctoral Dissertation). Reims. Retrieved from http://www.theses.fr/2011REIMS010
Chicago Manual of Style (16th Edition):
Harakat, Dominique. “Etudes mécanistiques par ESI-MS de réactions palladocatalysées : Mechanism studies of palladocatalyzed reactions by ESI-MS.” 2011. Doctoral Dissertation, Reims. Accessed April 18, 2021.
http://www.theses.fr/2011REIMS010.
MLA Handbook (7th Edition):
Harakat, Dominique. “Etudes mécanistiques par ESI-MS de réactions palladocatalysées : Mechanism studies of palladocatalyzed reactions by ESI-MS.” 2011. Web. 18 Apr 2021.
Vancouver:
Harakat D. Etudes mécanistiques par ESI-MS de réactions palladocatalysées : Mechanism studies of palladocatalyzed reactions by ESI-MS. [Internet] [Doctoral dissertation]. Reims; 2011. [cited 2021 Apr 18].
Available from: http://www.theses.fr/2011REIMS010.
Council of Science Editors:
Harakat D. Etudes mécanistiques par ESI-MS de réactions palladocatalysées : Mechanism studies of palladocatalyzed reactions by ESI-MS. [Doctoral Dissertation]. Reims; 2011. Available from: http://www.theses.fr/2011REIMS010
University of Debrecen
7.
Razavi, Hamid.
Natalizumab a Monoclonal Antibody in treatment of Multiple Sclerosis
.
Degree: DE – Általános Orvostudományi Kar, 2014, University of Debrecen
URL: http://hdl.handle.net/2437/194846
Subjects/Keywords: Nat;
MS
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APA (6th Edition):
Razavi, H. (2014). Natalizumab a Monoclonal Antibody in treatment of Multiple Sclerosis
. (Thesis). University of Debrecen. Retrieved from http://hdl.handle.net/2437/194846
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Razavi, Hamid. “Natalizumab a Monoclonal Antibody in treatment of Multiple Sclerosis
.” 2014. Thesis, University of Debrecen. Accessed April 18, 2021.
http://hdl.handle.net/2437/194846.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Razavi, Hamid. “Natalizumab a Monoclonal Antibody in treatment of Multiple Sclerosis
.” 2014. Web. 18 Apr 2021.
Vancouver:
Razavi H. Natalizumab a Monoclonal Antibody in treatment of Multiple Sclerosis
. [Internet] [Thesis]. University of Debrecen; 2014. [cited 2021 Apr 18].
Available from: http://hdl.handle.net/2437/194846.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Razavi H. Natalizumab a Monoclonal Antibody in treatment of Multiple Sclerosis
. [Thesis]. University of Debrecen; 2014. Available from: http://hdl.handle.net/2437/194846
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
North-West University
8.
Van der Westhuizen, Jacobus Cornelius.
Implementing an LC-QQQ method for the quantification of vitamin D analogues from serum accounting for epimers and isobars / Jacobus Cornelius van der Westhuizen
.
Degree: 2014, North-West University
URL: http://hdl.handle.net/10394/14893
► In the early 19th century a ground-breaking discovery was made that linked a dietary deficiency of a fat-soluble vitamin with the childhood disease known as…
(more)
▼ In the early 19th century a ground-breaking discovery was made that linked a dietary
deficiency of a fat-soluble vitamin with the childhood disease known as rickets. The vitamin
was named vitamin D and extensive research regarding the physiological importance of this
vitamin followed ever since. It is currently known that vitamin D plays an important role in
maintaining the calcium and phosphate homeostasis in the human body. Less clear
evidence states the medical importance of vitamin D in the prevention and cancer,
autoimmune disease and diabetes. Current literature shows that vitamin D has five distinct
forms, vitamin D1 to D5, of which vitamin D2 and D3 are the most studied forms. The term
“vitamin D” is often wrongfully used to include the vitamin D mother molecule, the vitamin D
status indicator (25(OH)D), the biologically active form (1,25(OH)2D) and biologically inactive
form (24,25(OH)2D). The interest for measurement of these vitamin D analogues is a
continuously growing field both on individual and epidemiological level. For decades
laboratories have struggled to produce a robust method capable of quantifying these
different vitamin D analogues and uncovered a new form of complexity regarding the
analysis of these analogues. The identification of the C3-epimeric forms of vitamin D
metabolites has forced laboratories to rethink their analytical methods and several concerns
were raised regarding the overestimation of the true vitamin D status by current analytical
methods. The quantification of the biologically active and inactive forms of vitamin D is
reported to be difficult and to date very few LC-MS/MS methods reported in the literature are
able to quantify various vitamin D analogues. However, to our knowledge none of these
methods are able to include the precursor vitamin D, the 25-hydroxylated metabolites, the
biologically active and inactive metabolites, C3-epimers and isobaric compounds in a single
run.
Therefore the aim of this study was to develop, optimise and validate a LC-MS/MS method
for the quantification of twelve vitamin D analogues in a single run. This was done by
optimising the underlying LC-MS/MS parameters to ensure optimal analytical sensitivity in
positive ESI mode and sufficient chromatographic separation between analytes with similar
chemical properties. Furthermore, the optimised method was validated to ensure the
accuracy and precision of the method before implementation into a clinical environment. The
vitamin D analogues included in this study were vitamin D2, vitamin D3, 25(OH)D2,
25(OH)D3, 1,25(OH)2D2, 1,25(OH)2D3, 24,25(OH)2D2, 24,25(OH)2D3, 3-epi-25(OH)D2, 3-epi-
25(OH)D3, 7(OH)4C3 and 1α(OH)D3.
A double liquid-liquid extraction with hexane and ethyl acetate were found to be the most
efficient at extracting the vitamin D analogues from a serum matrix after matrix modification with sodium hydroxide. Recoveries of > 95 % (CV <10 %) were achieved for all the analytes.
It was noted that a precursor adduct other than the molecular mass ion for a…
Subjects/Keywords: Vitamin D;
LC-MS/MS optimisation;
LC-MS/MS method validation
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APA ·
Chicago ·
MLA ·
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CSE |
Export
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APA (6th Edition):
Van der Westhuizen, J. C. (2014). Implementing an LC-QQQ method for the quantification of vitamin D analogues from serum accounting for epimers and isobars / Jacobus Cornelius van der Westhuizen
. (Thesis). North-West University. Retrieved from http://hdl.handle.net/10394/14893
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Van der Westhuizen, Jacobus Cornelius. “Implementing an LC-QQQ method for the quantification of vitamin D analogues from serum accounting for epimers and isobars / Jacobus Cornelius van der Westhuizen
.” 2014. Thesis, North-West University. Accessed April 18, 2021.
http://hdl.handle.net/10394/14893.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Van der Westhuizen, Jacobus Cornelius. “Implementing an LC-QQQ method for the quantification of vitamin D analogues from serum accounting for epimers and isobars / Jacobus Cornelius van der Westhuizen
.” 2014. Web. 18 Apr 2021.
Vancouver:
Van der Westhuizen JC. Implementing an LC-QQQ method for the quantification of vitamin D analogues from serum accounting for epimers and isobars / Jacobus Cornelius van der Westhuizen
. [Internet] [Thesis]. North-West University; 2014. [cited 2021 Apr 18].
Available from: http://hdl.handle.net/10394/14893.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Van der Westhuizen JC. Implementing an LC-QQQ method for the quantification of vitamin D analogues from serum accounting for epimers and isobars / Jacobus Cornelius van der Westhuizen
. [Thesis]. North-West University; 2014. Available from: http://hdl.handle.net/10394/14893
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
California State Polytechnic University – Pomona
9.
Tam, Louis.
Determination of Fatty Acid Profiles in Submerged Rice Using GC-MS.
Degree: MS, Department of Chemistry and Biochemistry, 2020, California State Polytechnic University – Pomona
URL: http://hdl.handle.net/10211.3/214925
► Natural disasters severely affect crop production and main food sources for global populations. Deep and prolonged flooding is one type of abiotic stress, which has…
(more)
▼ Natural disasters severely affect crop production and main food sources for global populations. Deep and prolonged flooding is one type of abiotic stress, which has a large and deleterious impact on rice production. Previous studies have shown that rice containing the SUB1A gene generally have a better survival rate compared to those without the SUB1A gene. Fatty acids are not only contribute to providing energy plants need, but also play an important role in plant self-defence. Obtaining the fatty acids profiles in rice that are both submergence tolerant and intolerant will provide the valuable insight on how they response to flooding. In this study, a GC-
MS method is developed to compare the fatty acids profiles between Oryza sativa ssp. Japonica cv. M202 and cv. M202 (SUB1) with the submergence stress for 24 hours. The increase of percentage for palmitic acid (shoot and root) and linoleic acid (shoot only) in M202 (SUB1) is larger than M202 after 24 hours submergence compared with the control. This is possibly due to M202 (SUB1) having a faster response to submergence stress within same period of time. Data also show ?? -linolenic acid levels decrease in M202 (SUB1) and increase in M202 samples, indicating a genotype-dependent response.
Advisors/Committee Members: Barding, Gregory (advisor), Barding, Gregory (editor), Stieber, Chantal (committee member).
Subjects/Keywords: GC-MS
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APA (6th Edition):
Tam, L. (2020). Determination of Fatty Acid Profiles in Submerged Rice Using GC-MS. (Masters Thesis). California State Polytechnic University – Pomona. Retrieved from http://hdl.handle.net/10211.3/214925
Chicago Manual of Style (16th Edition):
Tam, Louis. “Determination of Fatty Acid Profiles in Submerged Rice Using GC-MS.” 2020. Masters Thesis, California State Polytechnic University – Pomona. Accessed April 18, 2021.
http://hdl.handle.net/10211.3/214925.
MLA Handbook (7th Edition):
Tam, Louis. “Determination of Fatty Acid Profiles in Submerged Rice Using GC-MS.” 2020. Web. 18 Apr 2021.
Vancouver:
Tam L. Determination of Fatty Acid Profiles in Submerged Rice Using GC-MS. [Internet] [Masters thesis]. California State Polytechnic University – Pomona; 2020. [cited 2021 Apr 18].
Available from: http://hdl.handle.net/10211.3/214925.
Council of Science Editors:
Tam L. Determination of Fatty Acid Profiles in Submerged Rice Using GC-MS. [Masters Thesis]. California State Polytechnic University – Pomona; 2020. Available from: http://hdl.handle.net/10211.3/214925
Brno University of Technology
10.
Měřínská, Radana.
Studium fenolických látek ve vybraných biologických materiálech s využitím metody LC/MS: Study of phenolic compounds in some biologic materials using method LC/MS.
Degree: 2019, Brno University of Technology
URL: http://hdl.handle.net/11012/7304
► Presented bachelor thesis was focused on suitable methods for analysis of phenolics, especially modern instrumental methods were studied. In experimental part some standard phenolic compounds…
(more)
▼ Presented bachelor thesis was focused on suitable methods for analysis of phenolics, especially modern instrumental methods were studied. In experimental part some standard phenolic compounds were analysed using combined technique HPLC/ESI-
MS with off-line and/or on-line detection. Mass spectra of individual stadards and/or mixtures were determined in
MS full scan and/or
MS/MS full scan mode. Ionisation of chosen standard compounds was done in pozitive and/or negative mode. Parent ions of followed compounds were identified: reserpine (PI 609.9, NI 607.7), acid chlorogenic (NI 353), quercetin (PI 303.5, NI 301.5), rutin (PI 611, NI 609), morin (PI 303.1, NI 301.1). Typical fragments of studied standards were produced using
MS/MS full scan mode. By fragmentation characteristic product ions were identified: acid chlorogenic (NI 191), quercetin (PI: 229.1 and 257.1; NI: 179.0 and 151.0), rutin (in
MS/MS NI: 301; in MS3 NI: 179, 151.1), morin (PI: 285.1 a 257.1; NI: 283.2, 273.2, 257.1, 229.1). Optimal conditions of phenolic HPLC separation were determined including gradient elution and Restek column use. In conclusion on-line HPLC/
MS detection using negative ionization was observed as the best configuration for phenolic analysis.
Advisors/Committee Members: Kočí, Radka (advisor), Zemanová, Jana (referee).
Subjects/Keywords: HPLC/UV-VIS; LC/MS; LC/MS/MS; polyfenoly; HPLC/UV-VIS; LC/MS; LC/MS/MS; phenolics
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APA ·
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APA (6th Edition):
Měřínská, R. (2019). Studium fenolických látek ve vybraných biologických materiálech s využitím metody LC/MS: Study of phenolic compounds in some biologic materials using method LC/MS. (Thesis). Brno University of Technology. Retrieved from http://hdl.handle.net/11012/7304
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Měřínská, Radana. “Studium fenolických látek ve vybraných biologických materiálech s využitím metody LC/MS: Study of phenolic compounds in some biologic materials using method LC/MS.” 2019. Thesis, Brno University of Technology. Accessed April 18, 2021.
http://hdl.handle.net/11012/7304.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Měřínská, Radana. “Studium fenolických látek ve vybraných biologických materiálech s využitím metody LC/MS: Study of phenolic compounds in some biologic materials using method LC/MS.” 2019. Web. 18 Apr 2021.
Vancouver:
Měřínská R. Studium fenolických látek ve vybraných biologických materiálech s využitím metody LC/MS: Study of phenolic compounds in some biologic materials using method LC/MS. [Internet] [Thesis]. Brno University of Technology; 2019. [cited 2021 Apr 18].
Available from: http://hdl.handle.net/11012/7304.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Měřínská R. Studium fenolických látek ve vybraných biologických materiálech s využitím metody LC/MS: Study of phenolic compounds in some biologic materials using method LC/MS. [Thesis]. Brno University of Technology; 2019. Available from: http://hdl.handle.net/11012/7304
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Universidade Estadual de Campinas
11.
Oliveira, Wellington da Silva, 1988-.
Additives, aromatic amines, metals and nitrosamines in baby bottles : analytical methodologies and migration studies = Aditivos, aminas aromáticas, metais e nitrosaminas em mamadeiras: metodologias analíticas e estudos de migração.
Degree: Faculdade de Engenharia de Alimentos; Universidade de Zaragoza; Programa de Pós-Graduação em Ciência de Alimentos, 2018, Universidade Estadual de Campinas
URL: http://repositorio.unicamp.br/jspui/handle/REPOSIP/334730
► Orientadores: Helena Teixeira Godoy, Cristina Nerin, Marisa Padula
Tese (doutorado) - Universidade Estadual de Campinas, Faculdade de Engenharia de Alimentos e Universidade de Zaragoza
Made…
(more)
▼ Orientadores: Helena Teixeira Godoy, Cristina Nerin, Marisa Padula
Tese (doutorado) - Universidade Estadual de Campinas, Faculdade de Engenharia de Alimentos e Universidade de Zaragoza
Made available in DSpace on 2019-08-21T17:22:29Z (GMT). No. of bitstreams: 1 Oliveira_WellingtonDaSilva_D.pdf: 2120039 bytes, checksum: 77399662f3d0ac577f5c0d5dbca6b819 (MD5) Previous issue date: 2018
Resumo: O objetivo desse estudo foi avaliar a migração de compostos das mamadeiras, por meio da cromatografia líquida, gasosa e da espectroscopia, acopladas a espectrometria de massas de alta e baixa resolução. Foram avaliadas 196 amostras de mamadeiras fabricadas com polipropileno (n = 127), Tritan® (n = 49) e silicone (n = 20). O trabalho está dividido em quatro capítulos. O primeiro trata da caracterização das mamadeiras, da avaliação da migração específica de ftalatos por GC-MS utilizando fórmula infantil e simulante de leite (etanol 50%), bem como da
avaliação da migração de metais por ICP-OES em simulante de alimentos ácidos (ácido acético 3%). A migração de metais não foi detectada. Por outro lado, em todas as amostras foi detectada a migração de dibutil ftalato (DBP). Na amostra de silicone a migração de DBP foi superior aos valores preconizados pela ANVISA. Di-isobutil ftalato, um plastificante não regulamentado para uso em mamadeiras, também foi detectado em altas concentrações nas amostras de silicone. A avaliação de risco para ftalatos demonstrou que há um potencial de efeito adverso associado ao uso das mamadeiras de silicone. O segundo capítulo trata da migração de aminas aromáticas das mamadeiras por LC-MS/MS, bem como da migração de metais a partir dos bicos, avaliada por ICP-MS e N-nitrosaminas, determinada por GC-MS. Não foi detectada a migração de aminas aromáticas e N-nitrosaminas. Por outro lado, zinco e bário, foram quantificados em 91% das amostras analisadas. Chumbo foi detectado em 16% dos bicos avaliados. Os
metais migraram abaixo dos limites de migração específica preconizados pela ANVISA. No terceiro capítulo foi realizada a avaliação de compostos não voláteis, não intencionalmente adicionadas, utilizando UPLC-Q-TOF-MS. Em mamadeiras de polipropileno e Tritan® foram identificadas somente substâncias adicionadas intencionalmente (aditivos e auxiliares de processamento). Em todas as mamadeiras de polipropileno foi detectada a migração de N,N'-bis(2-hidroxietil)alquil(C8-C18)amina. Além disso, foram identificados derivados de glicerol, erucamida e NX 8000, um clarificante a base de nonitol. Todos os compostos migraram abaixo dos limites de migração específica preconizados pela ANVISA. Por outro lado, nas mamadeiras de silicone, foram identificados 17 compostos não intencionalmente adicionados. A maioria dos compostos eram derivados de acrilatos e propileno glicol. N-acetil valina também foi identificado nessas amostras. Os compostos foram quantificados e uma vez que não são contemplados
nas listas positivas da ANVISA, a abordagem do limiar de preocupação toxicológica (Threshold of Toxicological Concern…
Advisors/Committee Members: UNIVERSIDADE ESTADUAL DE CAMPINAS, Padula, Marisa, Godoy, Helena Teixeira, 1957-, Bragotto, Adriana Pavesi Arisseto, Anjos, Carlos Alberto Rodrigues, Coltro, Leda, Wagner, Roger.
Subjects/Keywords: Migração; Ftalatos; GC-MS; LC-MS/MS; Mamadeiras; Migration; Phthalates; GC-MS; LC-MS/MS; Baby bottles
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APA (6th Edition):
Oliveira, Wellington da Silva, 1. (2018). Additives, aromatic amines, metals and nitrosamines in baby bottles : analytical methodologies and migration studies = Aditivos, aminas aromáticas, metais e nitrosaminas em mamadeiras: metodologias analíticas e estudos de migração. (Doctoral Dissertation). Universidade Estadual de Campinas. Retrieved from http://repositorio.unicamp.br/jspui/handle/REPOSIP/334730
Chicago Manual of Style (16th Edition):
Oliveira, Wellington da Silva, 1988-. “Additives, aromatic amines, metals and nitrosamines in baby bottles : analytical methodologies and migration studies = Aditivos, aminas aromáticas, metais e nitrosaminas em mamadeiras: metodologias analíticas e estudos de migração.” 2018. Doctoral Dissertation, Universidade Estadual de Campinas. Accessed April 18, 2021.
http://repositorio.unicamp.br/jspui/handle/REPOSIP/334730.
MLA Handbook (7th Edition):
Oliveira, Wellington da Silva, 1988-. “Additives, aromatic amines, metals and nitrosamines in baby bottles : analytical methodologies and migration studies = Aditivos, aminas aromáticas, metais e nitrosaminas em mamadeiras: metodologias analíticas e estudos de migração.” 2018. Web. 18 Apr 2021.
Vancouver:
Oliveira, Wellington da Silva 1. Additives, aromatic amines, metals and nitrosamines in baby bottles : analytical methodologies and migration studies = Aditivos, aminas aromáticas, metais e nitrosaminas em mamadeiras: metodologias analíticas e estudos de migração. [Internet] [Doctoral dissertation]. Universidade Estadual de Campinas; 2018. [cited 2021 Apr 18].
Available from: http://repositorio.unicamp.br/jspui/handle/REPOSIP/334730.
Council of Science Editors:
Oliveira, Wellington da Silva 1. Additives, aromatic amines, metals and nitrosamines in baby bottles : analytical methodologies and migration studies = Aditivos, aminas aromáticas, metais e nitrosaminas em mamadeiras: metodologias analíticas e estudos de migração. [Doctoral Dissertation]. Universidade Estadual de Campinas; 2018. Available from: http://repositorio.unicamp.br/jspui/handle/REPOSIP/334730
NSYSU
12.
Shieh, Chia-lin.
The biochemical studies of peroxidase in Wasabia japonica.
Degree: Master, Biological Sciences, 2008, NSYSU
URL: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0212108-142719
► The plant peroxidases (EC1.11.17) exit as a large family of isozymes. These isozymes have more than 50% amino acid sequence differences. The function of Wasaba…
(more)
▼ The plant peroxidases (EC1.11.17) exit as a large family of isozymes. These isozymes have more than 50% amino acid sequence differences. The function of Wasaba japonica peroxides plays the role as IAA oxidases. The kinetics result shows Wasabia japonica peroxidases displayed affinity (Km = 17.1 μM) for IAA. The kinetics results in Wasabia japonica peroxidases display affinity (Km = 80.6 μM) for syringaldazine. LC/
MS/MS technique described the data that has proven to be a method for identification and characterization of proteins. The soluble proteins extracted form Wasabia japonica was purified by gel filtration chromatography and two-dimensional gel electrophoresis (2-DE). LC-
MS/MS analyses of 2-DE gel spots and identify proteins structure based on the protein fragmentation characteristics. The Mascot Search Results showed that Wasabia japonica peroxidase has a significant similarity (10%) with Arabidopsis thaliana peroxidase.
Advisors/Committee Members: Chin-gme Hsu (chair), Zin -huang Lin (committee member), Man-gjye Ger (chair).
Subjects/Keywords: LC-MS/MS; Wasabia japonica; peroxidase
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Manager
APA (6th Edition):
Shieh, C. (2008). The biochemical studies of peroxidase in Wasabia japonica. (Thesis). NSYSU. Retrieved from http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0212108-142719
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Shieh, Chia-lin. “The biochemical studies of peroxidase in Wasabia japonica.” 2008. Thesis, NSYSU. Accessed April 18, 2021.
http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0212108-142719.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Shieh, Chia-lin. “The biochemical studies of peroxidase in Wasabia japonica.” 2008. Web. 18 Apr 2021.
Vancouver:
Shieh C. The biochemical studies of peroxidase in Wasabia japonica. [Internet] [Thesis]. NSYSU; 2008. [cited 2021 Apr 18].
Available from: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0212108-142719.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Shieh C. The biochemical studies of peroxidase in Wasabia japonica. [Thesis]. NSYSU; 2008. Available from: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0212108-142719
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
NSYSU
13.
Tsai, Chung-Wei.
Studies in Determination and Residues of Nitrofurans and Corresponding Metabolites by LC-MS/MS in Tilapia.
Degree: PhD, Marine Biotechnology and Resources, 2009, NSYSU
URL: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0824109-162815
► Nitrofurans have been widely used either in waterbath or feed additives for the prevention and treatment of aquatic products. The European Union was able to…
(more)
▼ Nitrofurans have been widely used either in waterbath or feed additives for the prevention and treatment of aquatic products. The European Union was able to assign a maximum residue limit and prohibited nitrofurans used to animals in 1995, because of the potential carcinogenic effects of their residues on human health. This study is focusing on the analytical method of four kinds of commonly used nitrofurans and corresponding residual metabolites by LC-
MS/MS. The detection limits of furazolidone, furaltadone, nitrofurazone and nitrofurntoin were 6.11, 3.63, 4.52 and 6.20 μg kg-1,respectively. The detection limits of AOZ, AMOZ, SC and AH were 0.23, 0.30, 0.36, 0.53 μg kg-1, respectively. The lightness is the main factor to cause the decomposition of nitrofurans. It is not significant for temperature to depredate nitrofurans. The adsorbtion of metabolites by the plastic tube was in the extraction procedure. Equipments in glass are suggested to be used for the sample pretreatment and plastic meterials are averted to be exercised.
About the comparation of determination of AOZ by ELISA and LC-
MS/MS. The result demonstrated that the ELISA method might overestimate the residual AOZ content at low concentrations. The detection limit and recovery of the known addition were 0.05 μg kg-1 and 108% for the LC-
MS/MS method and 0.31 μg kg-1 and 305% for the ELISA method, respectively.
The amounts of residual nitrofurans and metabolites in muscle, liver, gill and skin tissue of tilapia which were treated in different conditions were compared. The depletion data of bathing treatment group obtained showed similar be haviors of furazolidone, furaltadone, nitrofurazone, nitrofurantoin in tilapia which the residual time was less than 24 hr. The amounts of residual nitrofurans appeared the highest concentration in gill and the lowest concentration in muscle. Bonded residues of metabolites can be detected for at least 4 weeks after administration in muscle, skin, liver and gill. The concentrations of residual bonded metabolites were higher than non-bonded metabolites in gill and muscle besides liver during depletion periods. After bathing medication, there were more residual nitrofurans and corresponding metabolites in sea water tilapia than fresh water group, because sea water fish survives in high osmotic condition to reduce their urination. Nitrofurans and metabolites were deconstructed by enzyme in gills, livers, intestines and muscles. Then tissues of fish accumulated nitrofurans and metabolites soon after medication. The maturity of fish is one of facters to effect different residual concentration during depletion periods. Liver is the main tissue to deconstruct nitrofurans and metabolites for the bathing medication and intestine is the major tissue to decompose antibiotics for the feeding medicaton.
In this research, we built a completed way to determine nitrofurans and corresponding metatbolites. Comparation of fish in different conditions and different medicative ways were in this investigation. These results could be helpful…
Advisors/Committee Members: I-Ming Chen (committee member), Meng-Hsien Chen (chair), Wei-Hsien Wang (committee member), Chi-Hsin Hsu (chair), Mao-Sung Kuo (chair), Chin-En Tsai (chair).
Subjects/Keywords: LC-MS/MS; nitrofuran; metabolite; furazolidone
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APA (6th Edition):
Tsai, C. (2009). Studies in Determination and Residues of Nitrofurans and Corresponding Metabolites by LC-MS/MS in Tilapia. (Doctoral Dissertation). NSYSU. Retrieved from http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0824109-162815
Chicago Manual of Style (16th Edition):
Tsai, Chung-Wei. “Studies in Determination and Residues of Nitrofurans and Corresponding Metabolites by LC-MS/MS in Tilapia.” 2009. Doctoral Dissertation, NSYSU. Accessed April 18, 2021.
http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0824109-162815.
MLA Handbook (7th Edition):
Tsai, Chung-Wei. “Studies in Determination and Residues of Nitrofurans and Corresponding Metabolites by LC-MS/MS in Tilapia.” 2009. Web. 18 Apr 2021.
Vancouver:
Tsai C. Studies in Determination and Residues of Nitrofurans and Corresponding Metabolites by LC-MS/MS in Tilapia. [Internet] [Doctoral dissertation]. NSYSU; 2009. [cited 2021 Apr 18].
Available from: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0824109-162815.
Council of Science Editors:
Tsai C. Studies in Determination and Residues of Nitrofurans and Corresponding Metabolites by LC-MS/MS in Tilapia. [Doctoral Dissertation]. NSYSU; 2009. Available from: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0824109-162815
NSYSU
14.
Chen, Yu-chieh.
Determination of Macrolide and Lincoamide Antibiotic in Fish Muscle by High Performance Liquid Chromatography- Tandem Mass Spectrometry.
Degree: Master, Marine Biotechnology and Resources, 2010, NSYSU
URL: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0827110-160839
► The main research of this thesis includes three sections. The purpose of first part is to develop a liquid chromatography-tandem mass spectrometry (LC-MS/MS) method for…
(more)
▼ The main research of this thesis includes three sections. The purpose of first part is to develop a liquid chromatography-tandem mass spectrometry (LC-
MS/MS) method for simultaneous determination of 8 macrolide antibiotics and lincosamides inside fish tissue, including erythromycin (ERM), oleandomycin (OLD), kitasamycin (KIT), tylosin (TYL), josamycin (JOS), spiramycin (SPM), tilmicosin (TIL), and lincomycin (LIN). Homogenized samples are first extracted with acetonitrile, dehydrated with sodium sulphate anhydrous, and then condensed. After the residue was redissolved in methanol and the extracts were partitioned with n-hexane to remove lipids, the sample is filterced and detected by LC/
MS-MS using chromatography columns of Agilent HC-C18 (5μm, 150 mm Ã4.6 mm). The mobile phase A was 5mM ammonium acetate containing 0.1% formic acid, while the mobile phase B was acetonitrile. The analysis of 8 macrolide antibiotics and lincosamides can be achieved within 10 minutes with electrospray ionization-tandem mass spectrometry in positive mode using multiple reaction monitoring (MRM) for simultaneous detection.
The second part is to verify the method by regulation of European Union (EU) resolution scheme (2002/657/EC). In the case where the drug is set as allowed drug, the recovery rate under gradient addition according to MRL is between 93.64% to 106.67%, and the CV is between 0.27% to 7.17%. In the case where the drug is set as prohibited drug, the recovery rate under gradient addition according to MRPL is between 96.35%~104.88%, and the CV is between 6.77%~13.91%. As a result, the decision limit (CCα) and the Detection capability (CCβ) of the 8 macrolide antibiotics and lincosamides is between 0.24 to 0.40μg kg-1 and 0.33 to 0.49μg kg-1.
The last section is to evaluate the stability of drugs in fish body under domestic preservation and process methods on fish, including refrigeration at -20â and cold storage at 4 â. The test is implemented by adding the drug into fish tissue according to MRL and detecting the antibiotics residue after regulated 40 days. Besides, the effect on activity of drug residue in fish body after boiling at 100 â is compared. The results show that the residual amount of spiramycin, josamycin, tilmicosin, and lincomycin is below 35% while that of erythromycin, oleandomycin, kitasamycin, and tylosin will be below 20%. Therefore, the drugs including erythromycin, josamycin, tylosin, and lincomycin will stay stably in fish tissue if they are stored under -20 â. However, it may affect human health if the fish contains such antibiotic residues is not boiled.
Advisors/Committee Members: Chi-Hsin Hsu (chair), Wei-Hsien Wang (committee member), Jung-Hui Chen (chair).
Subjects/Keywords: Macrolide; Antibiotics; LC-MS/MS; Lincomycin
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APA (6th Edition):
Chen, Y. (2010). Determination of Macrolide and Lincoamide Antibiotic in Fish Muscle by High Performance Liquid Chromatography- Tandem Mass Spectrometry. (Thesis). NSYSU. Retrieved from http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0827110-160839
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Chen, Yu-chieh. “Determination of Macrolide and Lincoamide Antibiotic in Fish Muscle by High Performance Liquid Chromatography- Tandem Mass Spectrometry.” 2010. Thesis, NSYSU. Accessed April 18, 2021.
http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0827110-160839.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Chen, Yu-chieh. “Determination of Macrolide and Lincoamide Antibiotic in Fish Muscle by High Performance Liquid Chromatography- Tandem Mass Spectrometry.” 2010. Web. 18 Apr 2021.
Vancouver:
Chen Y. Determination of Macrolide and Lincoamide Antibiotic in Fish Muscle by High Performance Liquid Chromatography- Tandem Mass Spectrometry. [Internet] [Thesis]. NSYSU; 2010. [cited 2021 Apr 18].
Available from: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0827110-160839.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Chen Y. Determination of Macrolide and Lincoamide Antibiotic in Fish Muscle by High Performance Liquid Chromatography- Tandem Mass Spectrometry. [Thesis]. NSYSU; 2010. Available from: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0827110-160839
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
NSYSU
15.
Liu, Heng-Yan.
Identification and quantification of arsenic and selenium species in human urine and chromium and arsenic species in dietary supplement by HPLC-ICP-MS and HPLC-ESI-MS.
Degree: Master, Chemistry, 2013, NSYSU
URL: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0630113-223124
► First research, speciation of arsenic and selenium in human urine was carried out using ion chromatography (IC) for separation and inductively coupled mass spectrometry (ICP-MS)…
(more)
▼ First research, speciation of arsenic and selenium in human urine was carried out using ion chromatography (IC) for separation and inductively coupled mass spectrometry (ICP-
MS) for detection. The arsenic and selenium species studied were arsenite [As(III)], arsenate [As(V)], monomethylarsonic acid (MMA), dimethylarsinic acid (DMA), arsenobetaine (AsB) and selenite [Se(IV)], selenate [Se(IV)]. Chromatographic separation of all the species was achieved in <11 min in gradient elution mode using (NH4)2CO3 and methanol at pH 8.8. The outlet of the IC column was directly connected to the nebulizer of ICP-
MS for the determination of arsenic and selenium. The speciation of arsenic and selenium have been carried out in human urine samples. The recoveries from spiked samples were in the range of 91â109%. The unknown compounds detected in human urine were identified by coupling IC directly with electrospray ionizationâmass spectrometry (ESI-
MS). Selenosugar was identified in human urine. The limits of detection were in the range of 0.008â0.015 ng mLâ1 for various arsenic species and 0.022-0.030 ng mLâ1 for various selenium species based on peak height.
Second research, a HPLC separation procedure has been developed for the speciation of Cr(III), Cr(VI) and As(III), As(V), DMA, MMA, AsB in dietary supplement samples. The species were separated on anion-exchange LC in gradient elution mode. The mobile phase consisting of EDTA and (NH4)2CO3 at pH 8.8, operated in a linear ramp, yielded well resolved chromatograms of all the species within 14 min with retention times of less than 2% RSD. The analyses were carried out using dynamic reaction cellinductively coupled plasma-mass spectrometer (DRC-ICP-
MS). The DRC conditions have also been optimized to obtained interference free measurements of 52Cr, 53Cr and 75As, which were otherwise interfered by: 35Cl16O1H, 40Ar12C on 52Cr; 37Cl16O, 40Ar13C, 40Ar12C1H on 53Cr; and 40Ar35Cl on 75As. The detection limits of the procedure were 0.008-0.019 ng As mL-1 and 0.084-0.087 ng Cr mL-1. The accuracy of the method has been validated by comparing the sum of the concentrations obtained for individual species with total concentration of the elements in NRCC SRM DORM-3 Fish Protein. The method has also been applied on three real samples, diabetic support formula tablets, diabetic support formula dietary fiber and Spirulina tablets , in which case the comparison has been made with total concentrations determined after complete dissolution of the samples. In this study, a microwave-assisted extraction method was used for the extraction of chromium and arsenic species from dietary supplemen samples. The extraction efficiency was better than 88% and the recoveries from spiked samples were in the range of 91â103%.
Advisors/Committee Members: Wei-Lung Tseng (chair), Shiuh-jen Jiang (committee member), Shou-Mei Wu (chair).
Subjects/Keywords: ESI-MS; Arsenic; Selenium; Chromium; ICP-MS
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CSE |
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APA (6th Edition):
Liu, H. (2013). Identification and quantification of arsenic and selenium species in human urine and chromium and arsenic species in dietary supplement by HPLC-ICP-MS and HPLC-ESI-MS. (Thesis). NSYSU. Retrieved from http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0630113-223124
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Liu, Heng-Yan. “Identification and quantification of arsenic and selenium species in human urine and chromium and arsenic species in dietary supplement by HPLC-ICP-MS and HPLC-ESI-MS.” 2013. Thesis, NSYSU. Accessed April 18, 2021.
http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0630113-223124.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Liu, Heng-Yan. “Identification and quantification of arsenic and selenium species in human urine and chromium and arsenic species in dietary supplement by HPLC-ICP-MS and HPLC-ESI-MS.” 2013. Web. 18 Apr 2021.
Vancouver:
Liu H. Identification and quantification of arsenic and selenium species in human urine and chromium and arsenic species in dietary supplement by HPLC-ICP-MS and HPLC-ESI-MS. [Internet] [Thesis]. NSYSU; 2013. [cited 2021 Apr 18].
Available from: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0630113-223124.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Liu H. Identification and quantification of arsenic and selenium species in human urine and chromium and arsenic species in dietary supplement by HPLC-ICP-MS and HPLC-ESI-MS. [Thesis]. NSYSU; 2013. Available from: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0630113-223124
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
University of Utah
16.
Blamires, Takara L.
High sensitivity measurement of androgens by two-dimensional-high pressure liquid chromatography-tandem mass spectrometry.
Degree: MS;, Pathology;, 2010, University of Utah
URL: http://content.lib.utah.edu/cdm/singleitem/collection/etd2/id/1699/rec/577
► Dehydroepiandrosterone and androstenedione are precursors of sex hormones; testosterone is a bioactive precursor of steroids responsible for development of secondary male characteristics. Abnormal androgen production…
(more)
▼ Dehydroepiandrosterone and androstenedione are precursors of sex hormones; testosterone is a bioactive precursor of steroids responsible for development of secondary male characteristics. Abnormal androgen production may cause changes in gender-specific physiology and measurements are useful in diagnosing androgen and estrogen related disorders. We developed a high sensitivity two-dimensional liquid chromatography tandem mass spectrometry (LC-MS/MS) method for measurement of dehydroepiandrosterone, androstenedione, and testosterone. Stable isotope-labeled internal standards were added to 200 ?L of serum and extracted into methyl-tert-butyl ether. The solvent was evaporated and steroids derivatized with hydroxylamine. Instrumental analysis was performed on an API 4000 (Applied Biosystems) LC-MS/MS utilizing a TurboIonsprayTM source in positive multiple reaction monitoring (MRM) mode. Quantitation was performed with AnalystTM software. The quantitative MRM transitions monitored were (m/z): 304 to 253 Da for DHEA; 306 to 255 Da for d2-DHEA; 317 to 124 Da for androstenedione; 324 to 128 Da for d7-androstenedione; 304 to 124 Da for testosterone; and 307 to 112 Da for d3-testosterone. Two-dimensional chromatographic separation was performed using a Phenomenex Gemini C6 cartridge as the first dimension and a Phenomenex Gemini C18 column as the second dimension. Injection-to-injection interval was 4.5 minutes. The assay was linear up to 9.2 ng/mL for DHEA, 42.5 ng/mL for androstenedione, and 25.8 ng/mL for testosterone.
Subjects/Keywords: Androgens; HPLC-MS/MS; Mass spectrometry
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APA (6th Edition):
Blamires, T. L. (2010). High sensitivity measurement of androgens by two-dimensional-high pressure liquid chromatography-tandem mass spectrometry. (Masters Thesis). University of Utah. Retrieved from http://content.lib.utah.edu/cdm/singleitem/collection/etd2/id/1699/rec/577
Chicago Manual of Style (16th Edition):
Blamires, Takara L. “High sensitivity measurement of androgens by two-dimensional-high pressure liquid chromatography-tandem mass spectrometry.” 2010. Masters Thesis, University of Utah. Accessed April 18, 2021.
http://content.lib.utah.edu/cdm/singleitem/collection/etd2/id/1699/rec/577.
MLA Handbook (7th Edition):
Blamires, Takara L. “High sensitivity measurement of androgens by two-dimensional-high pressure liquid chromatography-tandem mass spectrometry.” 2010. Web. 18 Apr 2021.
Vancouver:
Blamires TL. High sensitivity measurement of androgens by two-dimensional-high pressure liquid chromatography-tandem mass spectrometry. [Internet] [Masters thesis]. University of Utah; 2010. [cited 2021 Apr 18].
Available from: http://content.lib.utah.edu/cdm/singleitem/collection/etd2/id/1699/rec/577.
Council of Science Editors:
Blamires TL. High sensitivity measurement of androgens by two-dimensional-high pressure liquid chromatography-tandem mass spectrometry. [Masters Thesis]. University of Utah; 2010. Available from: http://content.lib.utah.edu/cdm/singleitem/collection/etd2/id/1699/rec/577
University of Manchester
17.
Gupta, Pankaj Krishan Chand.
DIAGNOSTIC AND THERAPEUTIC APPLICATIONS OF LIQUID
CHROMATOGRAPHY-TANDEM MASS SPECTROMETRY (LC-MS/MS)-BASED URINE
ANALYSIS IN THE MANAGEMENT OF NON-ADHERENCE TO ANTIHYPERTENSIVE
TREATMENT.
Degree: 2019, University of Manchester
URL: http://www.manchester.ac.uk/escholar/uk-ac-man-scw:319466
► Non-adherence to medications is widespread in patients with chronic cardiovascular conditions of which hypertension is a prime example. Hypertension affects around 1.5 billion people worldwide,…
(more)
▼ Non-adherence to medications is widespread in
patients with chronic cardiovascular conditions of which
hypertension is a prime example. Hypertension affects around 1.5
billion people worldwide, but blood pressure is at target only in
the minority of patients despite the presence of cheap and
effective medications. One important reason for the lack of desired
response to blood pressure lowering therapy is the non-adherence to
antihypertensive medications. The recently developed biochemical
screening for adherence using urine or blood analysis by liquid
chromatography- tandem mass spectrometry (LC-
MS/MS) provides a
confirmation of presence of the most common antihypertensive
medications in a single sample with high precision, sensitivity and
specificity. The thesis contemplates the benefits of the objective
direct measurement of adherence versus other methods of screening
including questionnaires, pharmacy records, electronic monitoring
devices and directly observed therapy. It also thoroughly addresses
the current methodological, conceptual and interpretational
limitations of the biochemical screening for non-adherence to
antihypertensive treatment. The diagnostic potential of the test is
explored in a retrospective study of 1348 patients attending
clinical centers in the UK and the Czech Republic. The prevalence
of non-adherence to antihypertensive treatment was high at 41.6%
and 31.5% in these two populations, respectively. Non-adherence
rates showed a strong and almost linear relationship with the
number of prescribed antihypertensive medications, decreased with
increasing age, were higher in females and those on diuretics. The
therapeutic benefit of the test is demonstrated in a retrospective
cohort study of hypertensive patients with uncontrolled blood
pressure (BP) who had undergone a biochemical adherence testing.
The mean systolic blood pressure (SBP) dropped by 19.5 mmHg and
diastolic blood pressure (DBP) by 7.5 mmHg on follow up in the 73
initially non-adherent UK patients after the results of the test
were discussed with them. The corresponding figures for 93
initially non-adherent Czech patients were 32.6 mmHg (for SBP) and
17.6 mmHg for (DBP) on follow-up. The biological insights into
mechanisms of resistant hypertension are examined through the
analysis of associations between the degree of resistance to blood
pressure lowering therapy and clinical measures of sympathetic
nervous system activity. Resistant hypertension was proposed as a
state of the sympathetic nervous system over-activity, but this
concept was based on studies that did not exclude non- adherence to
antihypertensive treatment objectively. The analysis of 76 patients
with resistant hypertension demonstrated that if non-adherence is
excluded objectively by biochemical analysis of blood or urine,
there is no evidence of heightened activity of sympathetic nervous
system. The thesis assesses the overall clinical impact of the
biochemical screening for non- adherence to treatment in management
of hypertensive patients. It also outlines current…
Advisors/Committee Members: KEAVNEY, BERNARD B, Tomaszewski, Maciej, Keavney, Bernard.
Subjects/Keywords: non-adherence; hypertension; LC-MS/MS
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APA ·
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Export
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Manager
APA (6th Edition):
Gupta, P. K. C. (2019). DIAGNOSTIC AND THERAPEUTIC APPLICATIONS OF LIQUID
CHROMATOGRAPHY-TANDEM MASS SPECTROMETRY (LC-MS/MS)-BASED URINE
ANALYSIS IN THE MANAGEMENT OF NON-ADHERENCE TO ANTIHYPERTENSIVE
TREATMENT. (Doctoral Dissertation). University of Manchester. Retrieved from http://www.manchester.ac.uk/escholar/uk-ac-man-scw:319466
Chicago Manual of Style (16th Edition):
Gupta, Pankaj Krishan Chand. “DIAGNOSTIC AND THERAPEUTIC APPLICATIONS OF LIQUID
CHROMATOGRAPHY-TANDEM MASS SPECTROMETRY (LC-MS/MS)-BASED URINE
ANALYSIS IN THE MANAGEMENT OF NON-ADHERENCE TO ANTIHYPERTENSIVE
TREATMENT.” 2019. Doctoral Dissertation, University of Manchester. Accessed April 18, 2021.
http://www.manchester.ac.uk/escholar/uk-ac-man-scw:319466.
MLA Handbook (7th Edition):
Gupta, Pankaj Krishan Chand. “DIAGNOSTIC AND THERAPEUTIC APPLICATIONS OF LIQUID
CHROMATOGRAPHY-TANDEM MASS SPECTROMETRY (LC-MS/MS)-BASED URINE
ANALYSIS IN THE MANAGEMENT OF NON-ADHERENCE TO ANTIHYPERTENSIVE
TREATMENT.” 2019. Web. 18 Apr 2021.
Vancouver:
Gupta PKC. DIAGNOSTIC AND THERAPEUTIC APPLICATIONS OF LIQUID
CHROMATOGRAPHY-TANDEM MASS SPECTROMETRY (LC-MS/MS)-BASED URINE
ANALYSIS IN THE MANAGEMENT OF NON-ADHERENCE TO ANTIHYPERTENSIVE
TREATMENT. [Internet] [Doctoral dissertation]. University of Manchester; 2019. [cited 2021 Apr 18].
Available from: http://www.manchester.ac.uk/escholar/uk-ac-man-scw:319466.
Council of Science Editors:
Gupta PKC. DIAGNOSTIC AND THERAPEUTIC APPLICATIONS OF LIQUID
CHROMATOGRAPHY-TANDEM MASS SPECTROMETRY (LC-MS/MS)-BASED URINE
ANALYSIS IN THE MANAGEMENT OF NON-ADHERENCE TO ANTIHYPERTENSIVE
TREATMENT. [Doctoral Dissertation]. University of Manchester; 2019. Available from: http://www.manchester.ac.uk/escholar/uk-ac-man-scw:319466
University of Newcastle
18.
O'Hara, Kate.
New analytical techniques for determining pharmacokinetics of drugs in neonates.
Degree: PhD, 2020, University of Newcastle
URL: http://hdl.handle.net/1959.13/1412474
► Research Doctorate - Doctor of Philosophy (PhD)
Determining the appropriate dose of medication to use in a paediatric or neonatal patient is a clinical challenge.…
(more)
▼ Research Doctorate - Doctor of Philosophy (PhD)
Determining the appropriate dose of medication to use in a paediatric or neonatal patient is a clinical challenge. Many doses of medication currently used are extrapolated from adult dosing regimens due to a lack of pharmacokinetic studies in children and neonates. The lack of paediatric clinical trials and dosing information has been highlighted by many different international bodies, including the Food and Drug Administration (FDA) and European Medicines Agency (EMA) who acknowledged that this is an area of clinical need and there is now a requirement for more paediatric data in the licensing of new drugs. There is an urgent need for pharmacokinetic studies to be performed in paediatric and neonatal patients. Pharmacokinetic studies require the availability of a suitable assay to measure the drug concentration in blood or plasma. Without these analytical techniques, it is impossible to perform the required pharmacokinetic studies to develop dosing information in these patient groups. Neonates, in particular, have very small total blood volume and any samples taken must reflect this. The primary aim of this thesis is to describe the complex development of analytical techniques capable of measuring drug concentrations in small volume blood samples. Addressing this lack of suitable assays is a critical first step in pharmacokinetic research in this patient group. As part of the research performed for this thesis, a clinical study in neonates was conducted. From the experience of designing and conducting this clinical study in neonates, combined with a review of the literature, several barriers to this type of research were identified. These barriers included gaining ethics approval, parental consent issues, sufficient number of patients and multicentre trials, minimising blood sampling requirements and availability of suitable analytical techniques. Reflecting on these identified barriers, potential solutions to overcome these barriers have been proposed to assist researchers in the future. Similar to the adult population, the pharmacological therapies in neonatal patients span a huge range of medical conditions and illnesses. As a result of the review conducted for this thesis, priority areas requiring pharmacokinetic studies and further work were identified. This included analgesia, antibiotics and sedatives. In this thesis, small volume assays for drugs with clinical significance for neonatal patients in each of these areas were developed and validated as a first step towards determining pharmacokinetic data and evidence based dosing information. Remifentanil has been identified as a potentially useful analgesic in neonates. In this research, an assay capable of measuring concentrations as low as 0.25ng/mL in 100μL of plasma was developed using HPLC-MS/MS. Applicability to use in pharmacokinetic studies was demonstrated by analysing small volume samples to determine pharmacokinetics in a rabbit model. Remifentanil undergoes metabolism via hydrolysis by esterases.…
Advisors/Committee Members: University of Newcastle. Faculty of Health & Medicine, School of Medicine and Public Health.
Subjects/Keywords: HPLC-MS/MS; pharmacokinetics; neonates; paediatrics
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APA (6th Edition):
O'Hara, K. (2020). New analytical techniques for determining pharmacokinetics of drugs in neonates. (Doctoral Dissertation). University of Newcastle. Retrieved from http://hdl.handle.net/1959.13/1412474
Chicago Manual of Style (16th Edition):
O'Hara, Kate. “New analytical techniques for determining pharmacokinetics of drugs in neonates.” 2020. Doctoral Dissertation, University of Newcastle. Accessed April 18, 2021.
http://hdl.handle.net/1959.13/1412474.
MLA Handbook (7th Edition):
O'Hara, Kate. “New analytical techniques for determining pharmacokinetics of drugs in neonates.” 2020. Web. 18 Apr 2021.
Vancouver:
O'Hara K. New analytical techniques for determining pharmacokinetics of drugs in neonates. [Internet] [Doctoral dissertation]. University of Newcastle; 2020. [cited 2021 Apr 18].
Available from: http://hdl.handle.net/1959.13/1412474.
Council of Science Editors:
O'Hara K. New analytical techniques for determining pharmacokinetics of drugs in neonates. [Doctoral Dissertation]. University of Newcastle; 2020. Available from: http://hdl.handle.net/1959.13/1412474
University of Manchester
19.
Gupta, Pankaj.
Diagnostic and therapeutic applications of liquid chromatography-tandem mass spectrometry (LC-MS/MS)-based urine analysis in the management of non-adherence to antihypertensive treatment.
Degree: Thesis (M.D.), 2019, University of Manchester
URL: https://www.research.manchester.ac.uk/portal/en/theses/diagnostic-and-therapeutic-applications-of-liquid-chromatographytandem-mass-spectrometry-lcmsmsbased-urine-analysis-in-the-management-of-nonadherence-to-antihypertensive-treatment(d9599095-d979-44e5-a24b-fc0a961077de).html
;
https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.809393
► Non-adherence to medications is widespread in patients with chronic cardiovascular conditions of which hypertension is a prime example. Hypertension affects around 1.5 billion people worldwide,…
(more)
▼ Non-adherence to medications is widespread in patients with chronic cardiovascular conditions of which hypertension is a prime example. Hypertension affects around 1.5 billion people worldwide, but blood pressure is at target only in the minority of patients despite the presence of cheap and effective medications. One important reason for the lack of desired response to blood pressure lowering therapy is the non-adherence to antihypertensive medications. The recently developed biochemical screening for adherence using urine or blood analysis by liquid chromatography- tandem mass spectrometry (LC-MS/MS) provides a confirmation of presence of the most common antihypertensive medications in a single sample with high precision, sensitivity and specificity. The thesis contemplates the benefits of the objective direct measurement of adherence versus other methods of screening including questionnaires, pharmacy records, electronic monitoring devices and directly observed therapy. It also thoroughly addresses the current methodological, conceptual and interpretational limitations of the biochemical screening for non-adherence to antihypertensive treatment. The diagnostic potential of the test is explored in a retrospective study of 1348 patients attending clinical centers in the UK and the Czech Republic. The prevalence of non-adherence to antihypertensive treatment was high at 41.6% and 31.5% in these two populations, respectively. Non-adherence rates showed a strong and almost linear relationship with the number of prescribed antihypertensive medications, decreased with increasing age, were higher in females and those on diuretics. The therapeutic benefit of the test is demonstrated in a retrospective cohort study of hypertensive patients with uncontrolled blood pressure (BP) who had undergone a biochemical adherence testing. The mean systolic blood pressure (SBP) dropped by 19.5 mmHg and diastolic blood pressure (DBP) by 7.5 mmHg on follow up in the 73 initially non-adherent UK patients after the results of the test were discussed with them. The corresponding figures for 93 initially non-adherent Czech patients were 32.6 mmHg (for SBP) and 17.6 mmHg for (DBP) on follow-up. The biological insights into mechanisms of resistant hypertension are examined through the analysis of associations between the degree of resistance to blood pressure lowering therapy and clinical measures of sympathetic nervous system activity. Resistant hypertension was proposed as a state of the sympathetic nervous system over-activity, but this concept was based on studies that did not exclude non- adherence to antihypertensive treatment objectively. The analysis of 76 patients with resistant hypertension demonstrated that if non-adherence is excluded objectively by biochemical analysis of blood or urine, there is no evidence of heightened activity of sympathetic nervous system. The thesis assesses the overall clinical impact of the biochemical screening for non- adherence to treatment in management of hypertensive patients. It also outlines current…
Subjects/Keywords: hypertension; LC-MS/MS; non-adherence
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APA ·
Chicago ·
MLA ·
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CSE |
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to Zotero / EndNote / Reference
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APA (6th Edition):
Gupta, P. (2019). Diagnostic and therapeutic applications of liquid chromatography-tandem mass spectrometry (LC-MS/MS)-based urine analysis in the management of non-adherence to antihypertensive treatment. (Doctoral Dissertation). University of Manchester. Retrieved from https://www.research.manchester.ac.uk/portal/en/theses/diagnostic-and-therapeutic-applications-of-liquid-chromatographytandem-mass-spectrometry-lcmsmsbased-urine-analysis-in-the-management-of-nonadherence-to-antihypertensive-treatment(d9599095-d979-44e5-a24b-fc0a961077de).html ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.809393
Chicago Manual of Style (16th Edition):
Gupta, Pankaj. “Diagnostic and therapeutic applications of liquid chromatography-tandem mass spectrometry (LC-MS/MS)-based urine analysis in the management of non-adherence to antihypertensive treatment.” 2019. Doctoral Dissertation, University of Manchester. Accessed April 18, 2021.
https://www.research.manchester.ac.uk/portal/en/theses/diagnostic-and-therapeutic-applications-of-liquid-chromatographytandem-mass-spectrometry-lcmsmsbased-urine-analysis-in-the-management-of-nonadherence-to-antihypertensive-treatment(d9599095-d979-44e5-a24b-fc0a961077de).html ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.809393.
MLA Handbook (7th Edition):
Gupta, Pankaj. “Diagnostic and therapeutic applications of liquid chromatography-tandem mass spectrometry (LC-MS/MS)-based urine analysis in the management of non-adherence to antihypertensive treatment.” 2019. Web. 18 Apr 2021.
Vancouver:
Gupta P. Diagnostic and therapeutic applications of liquid chromatography-tandem mass spectrometry (LC-MS/MS)-based urine analysis in the management of non-adherence to antihypertensive treatment. [Internet] [Doctoral dissertation]. University of Manchester; 2019. [cited 2021 Apr 18].
Available from: https://www.research.manchester.ac.uk/portal/en/theses/diagnostic-and-therapeutic-applications-of-liquid-chromatographytandem-mass-spectrometry-lcmsmsbased-urine-analysis-in-the-management-of-nonadherence-to-antihypertensive-treatment(d9599095-d979-44e5-a24b-fc0a961077de).html ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.809393.
Council of Science Editors:
Gupta P. Diagnostic and therapeutic applications of liquid chromatography-tandem mass spectrometry (LC-MS/MS)-based urine analysis in the management of non-adherence to antihypertensive treatment. [Doctoral Dissertation]. University of Manchester; 2019. Available from: https://www.research.manchester.ac.uk/portal/en/theses/diagnostic-and-therapeutic-applications-of-liquid-chromatographytandem-mass-spectrometry-lcmsmsbased-urine-analysis-in-the-management-of-nonadherence-to-antihypertensive-treatment(d9599095-d979-44e5-a24b-fc0a961077de).html ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.809393
University of Manchester
20.
Phillips, Ashley.
Probing Early Stage Aggregates of Amyloidogenic Proteins
using Mass Spectrometry Based Methods.
Degree: 2016, University of Manchester
URL: http://www.manchester.ac.uk/escholar/uk-ac-man-scw:306171
► Mass Spectrometry (MS) and Ion Mobility – Mass Spectrometry (IM-MS) can be used to investigate protein structure and dynamics and are ideally positioned to study…
(more)
▼ Mass Spectrometry (
MS) and Ion Mobility – Mass
Spectrometry (IM-
MS) can be used to investigate protein structure
and dynamics and are ideally positioned to study intrinsically
disordered and amyloidogenic proteins, whose diverse conformational
space and/or oligomeric state is hard to track accurately. This
thesis uses hybrid
MS approaches including IM-
MS, Cross-linking
IM-
MS and ECD-FT-ICR
MS to probe the structure of alpha-Synuclein
and Amyloid-beta (Abeta). For alpha-Synuclein, the effect of
solution pH and ionisation polarity on the species observed by
MS
and IM-
MS is investigated. Conformational families observed by
Cross-linking IM-
MS provides a link between the solution and gas
phase structures of alpha-Synuclein observed here and our data
correlates with that reported by other groups.
MS, IM-
MS and HDX-
MS
are used to probe alpha-Synuclein during the early stages of
aggregation. A specific aggregation competent conformer is not
observed suggesting that the solution constituents remain
conformationally dynamic. We observe shifts in the species observed
by
MS and IM-
MS between samples and our data contributes to an
array of conflicting structural studies indicating that
alpha-Synuclein adopts a diverse range of species with significant
variation.For Abeta(1-42) and Abeta(1-40) Collision Induced
Unfolding and ETD/ETcaD demonstrate that Abeta(1-42) adopts a
compact conformation bound by intramolecular interactions. Changes
to the Abeta(1-42) and Abeta(1-40) ATDs following SID are
correlated to known structure influencing intermolecular
interactions and demonstrate the large structural difference
between Abeta(1-42) and Abeta(1-40) despite differing by only two
C-terminal amino acids. IM-
MS is used to classify the mode of
action of anti-aggregation drug candidates on Abeta(1-42). The
anti-aggregation capacity of the retro-inverso peptide, RI-OR2 is
shown to result from inducing the compaction or extension of
Abeta(1-42), preventing the adoption of an aggregation competent
structure. In contrast, the flavonoid Rutin is shown to act solely
through inducing Abeta(1-42) compaction.This thesis demonstrates
the power of
MS based methods to investigate the diverse range of
structures of intrinsically disordered aggregating proteins
implicated in disease.
Advisors/Committee Members: GOODACRE, ROY R, Goodacre, Roy, Barran, Perdita.
Subjects/Keywords: alpha-Synuclein; Amyloid-beta; MS; IM-MS
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APA ·
Chicago ·
MLA ·
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CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Phillips, A. (2016). Probing Early Stage Aggregates of Amyloidogenic Proteins
using Mass Spectrometry Based Methods. (Doctoral Dissertation). University of Manchester. Retrieved from http://www.manchester.ac.uk/escholar/uk-ac-man-scw:306171
Chicago Manual of Style (16th Edition):
Phillips, Ashley. “Probing Early Stage Aggregates of Amyloidogenic Proteins
using Mass Spectrometry Based Methods.” 2016. Doctoral Dissertation, University of Manchester. Accessed April 18, 2021.
http://www.manchester.ac.uk/escholar/uk-ac-man-scw:306171.
MLA Handbook (7th Edition):
Phillips, Ashley. “Probing Early Stage Aggregates of Amyloidogenic Proteins
using Mass Spectrometry Based Methods.” 2016. Web. 18 Apr 2021.
Vancouver:
Phillips A. Probing Early Stage Aggregates of Amyloidogenic Proteins
using Mass Spectrometry Based Methods. [Internet] [Doctoral dissertation]. University of Manchester; 2016. [cited 2021 Apr 18].
Available from: http://www.manchester.ac.uk/escholar/uk-ac-man-scw:306171.
Council of Science Editors:
Phillips A. Probing Early Stage Aggregates of Amyloidogenic Proteins
using Mass Spectrometry Based Methods. [Doctoral Dissertation]. University of Manchester; 2016. Available from: http://www.manchester.ac.uk/escholar/uk-ac-man-scw:306171
University of New South Wales
21.
Haron, Normah.
Bioactives in traditional Malaysian vegetables and their antioxidant activities.
Degree: Chemical Sciences & Engineering, 2013, University of New South Wales
URL: http://handle.unsw.edu.au/1959.4/53318
;
https://unsworks.unsw.edu.au/fapi/datastream/unsworks:12013/SOURCE01?view=true
► The aim of the present study was to develop a specific and quantitative analytical method to determine the individual concentrations of naturally occurring phenolic acids…
(more)
▼ The aim of the present study was to develop a specific and quantitative analytical method to determine the individual concentrations of naturally occurring phenolic acids and flavonoids and their metabolites in food; then evaluate the antioxidant capacity of the quantified phenolic acids using different assays. An LC-
MS/MS method for the quantification of 9 phenolic acids and 5 commonly present flavonoid compounds in plant foods was developed. Target analytes were the benzoic acid based phenolic acids - anisic, gallic, salicylic, protocatechuic, and vanillic acid, together with the cinnamic acid based phenolic acids - caffeic, p-coumaric, ferulic and sinapinic acid. As for the flavonoids, target analytes were apigenin, luteolin, kaempferol, quercetin and myricetin. These compounds were identified by retention times and comparison of electron impact mass spectra. Deuterium-labelled ferulic acid and 13C-labeled quercetin were used as the internal standards to achieve accurate quantification. Purification was accomplished using Strata® SDBL (styrene-divinylbenzene) cartridges for solid phase extraction (Phenomenex, US). Sample extracts were subjected to acid and/or alkaline hydrolysis to release the free phenolic acids and flavonoids from the ester and glycoside forms found in plant materials. The resolution and quantitation of all compounds were excellent with linear calibration curves over the range detection limit for each compound to 750 ng/µL and correlation coefficients (R2) greater than 0.9643. The intraday assay range of relative standard deviation (RSD) was 2.6-9.0 for high concentrations and 2.3-16.7 for low concentrations of a standard mixture. For interday assay, the relative standard deviation (RSD) ranges were 10.8-20.2 for high concentrations and 12.7-19.4 for low concentrations. Percentage recovery of analytes were satisfactory (72 to 128% respectively) and within the acceptable limits of bioanalytical method validation guidelines (FDA, 2001). The developed and validated method was applied to different types of Malaysian traditional vegetables, fresh (n=15). The results revealed that flavonoids are the most abundant polyphenols in plant extracts, followed by hydroxycinnamic acids of phenolic acids, mostly in bound form as esters or glycosides. Only hydrophilic antioxidant activities of all selected samples were examined using ORAC assay. In addition the antioxidant capacity of the identified free and bound phenolic acids content was measured using different assays including ORAC, ABTS, and DPPH assay (end-point assay and kinetic assay). The results indicate that the total phenolic acids content did not correlate positively with antioxidant activity, but the total flavonoid content was positively correlated with antioxidant activity. In conclusion the analytical method presented is robust, safe, and generally applicable to the analysis of free and bound phenolic acids and flavonoids in food samples.
Advisors/Committee Members: Arcot, Jayashree, Chemical Sciences & Engineering, Faculty of Engineering, UNSW, Bucknall, Martin, Biomedical Mass Spectrometry Unit, Faculty of Medicine, UNSW, Ismail, Amin, Universiti Putra Malaysia.
Subjects/Keywords: Flavonoids; LC-MS/MS; Phenolic acids
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APA ·
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MLA ·
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Manager
APA (6th Edition):
Haron, N. (2013). Bioactives in traditional Malaysian vegetables and their antioxidant activities. (Doctoral Dissertation). University of New South Wales. Retrieved from http://handle.unsw.edu.au/1959.4/53318 ; https://unsworks.unsw.edu.au/fapi/datastream/unsworks:12013/SOURCE01?view=true
Chicago Manual of Style (16th Edition):
Haron, Normah. “Bioactives in traditional Malaysian vegetables and their antioxidant activities.” 2013. Doctoral Dissertation, University of New South Wales. Accessed April 18, 2021.
http://handle.unsw.edu.au/1959.4/53318 ; https://unsworks.unsw.edu.au/fapi/datastream/unsworks:12013/SOURCE01?view=true.
MLA Handbook (7th Edition):
Haron, Normah. “Bioactives in traditional Malaysian vegetables and their antioxidant activities.” 2013. Web. 18 Apr 2021.
Vancouver:
Haron N. Bioactives in traditional Malaysian vegetables and their antioxidant activities. [Internet] [Doctoral dissertation]. University of New South Wales; 2013. [cited 2021 Apr 18].
Available from: http://handle.unsw.edu.au/1959.4/53318 ; https://unsworks.unsw.edu.au/fapi/datastream/unsworks:12013/SOURCE01?view=true.
Council of Science Editors:
Haron N. Bioactives in traditional Malaysian vegetables and their antioxidant activities. [Doctoral Dissertation]. University of New South Wales; 2013. Available from: http://handle.unsw.edu.au/1959.4/53318 ; https://unsworks.unsw.edu.au/fapi/datastream/unsworks:12013/SOURCE01?view=true
Oklahoma State University
22.
Lopez, McCayla.
Characterization of Cannabinoids in Cannabidiol (CBD) Products.
Degree: Forensic Sciences, 2019, Oklahoma State University
URL: http://hdl.handle.net/11244/321630
► The purpose of this research was to determine what cannabinoids other than cannabidiol (CBD) can be found in various CBD products, as well as determine…
(more)
▼ The purpose of this research was to determine what cannabinoids other than cannabidiol (CBD) can be found in various CBD products, as well as determine their legality. CBD is federally a Schedule I drug, although CBD products are legal for sale in the State of Oklahoma if they contain less than 0.3% tetrahydrocannabinol (THC). Many CBD products sold do not have all the cannabinoids in the product included on the label, which could be a health risk. The samples in this study were purchased from CBD shops in Oklahoma and provided to Oklahoma State University (OSU) for analysis. Liquid chromatography with ultra-violet detection and liquid chromatography with mass spectrometry were used to separate 12 different cannabinoids commonly found in the Cannabis plant. The results showed that all five products had a higher amount of CBD than any other cannabinoid. It was also found that when CBD was present, cannabidivarin (CBDV) was also present, although most packages did not indicate the presence of cannabinoids other than CBD. All products contained at least two cannabinoids, with the highest concentration being CBD. All products tested were determined to have false information on their packaging, although they are legal in the State of Oklahoma since the detectable level of THC was less than 0.3%.
Advisors/Committee Members: Wagner, Jarrad R. (advisor), Payton, Mark (committee member), Lewis, Russell (committee member).
Subjects/Keywords: cannabidiol; cannabinoids; lc-ms/ms; lc/uv
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MLA ·
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to Zotero / EndNote / Reference
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APA (6th Edition):
Lopez, M. (2019). Characterization of Cannabinoids in Cannabidiol (CBD) Products. (Thesis). Oklahoma State University. Retrieved from http://hdl.handle.net/11244/321630
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Lopez, McCayla. “Characterization of Cannabinoids in Cannabidiol (CBD) Products.” 2019. Thesis, Oklahoma State University. Accessed April 18, 2021.
http://hdl.handle.net/11244/321630.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Lopez, McCayla. “Characterization of Cannabinoids in Cannabidiol (CBD) Products.” 2019. Web. 18 Apr 2021.
Vancouver:
Lopez M. Characterization of Cannabinoids in Cannabidiol (CBD) Products. [Internet] [Thesis]. Oklahoma State University; 2019. [cited 2021 Apr 18].
Available from: http://hdl.handle.net/11244/321630.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Lopez M. Characterization of Cannabinoids in Cannabidiol (CBD) Products. [Thesis]. Oklahoma State University; 2019. Available from: http://hdl.handle.net/11244/321630
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
University of Waterloo
23.
Yu, Tianchunlu.
Developing Macrolipidomics Profiling Methods for Dietary Food Samples.
Degree: 2021, University of Waterloo
URL: http://hdl.handle.net/10012/16697
► Diet has a major impact on health and disease and poor nutrition increases the risk of chronic disease. The amount and type of dietary fat…
(more)
▼ Diet has a major impact on health and disease and poor nutrition increases the risk of chronic disease. The amount and type of dietary fat has been a key target for dietary guidelines and health policy. Dietary fat in the food databases and on food labels is usually analyzed and described by the fatty acid composition of the fat, but dietary fat is a mix of fatty acyl specific species of triacylglycerols (TG) with minor contributions from other complex lipids such as phospholipids. The actual native lipid species information can be captured with a “macrolipidomic” approach that characterizes the individual fatty acyl species of the highly abundant lipids of a biological sample and provides additional information and insight about the dietary form and source of important nutritional fatty acids such as omega-3 polyunsaturates. The overall purpose of this thesis was to assess the ability of and identify challenges with the use of a macrolipidomic analytical approach for the characterization of dietary lipids by analyzing food items and meals. Tandem mass spectrometry coupled to ultra-high-performance liquid chromatography was used to characterize the dietary lipid species in individual food items and meal homogenates and compared with traditional fatty acid profiles generated by gas chromatography-flame ionization detection and food profiles from the Canadian Nutrient File database. Analyses were performed on two highly consumed commercial plant oils (soybean and canola oils), a breakfast sandwich, and homogenates of traditional Canadian versions of breakfast, lunch, snack and dinner. The fatty acid profiles of the plant oils differed with 18:2n-6 (54 wt%) being the main fatty acid in soybean oil and 18:1n-9 (62 wt%) in canola oil but there were no fatty acids detected that were unique to an oil. Lipidomic analyses revealed the two oils shared only 56 identified acyl lipid species with soybean oil having 105 and canola oil having 105 unique lipids. For the sandwich and meal analyses, that the fatty acid content by biochemical determination was lower than the Canadian Nutrient File estimates. Blending and homogenizing large volumes of mixed food items was a challenge and requires additional processing strategies in the future that need to be evaluated. The macrolipidomic analysis of the breakfast sandwich and the meals confirmed phospholipid content in the diet with egg likely being the main source, but there were challenges with quantitation. Macrolipidomics estimated that 2.7% breakfast sandwich lipid was phospholipid while gas chromatography determinations estimated the 17% of the fatty acids were associated with phospholipids. The extent of the lipidomic profiling was also limited by challenges in making correct fatty acyl lipid species identifications as the processing software was unable to import MS/MS data acquired by data dependent acquisition. Despite this, it was estimated that in meals that mimic a typical Canadian diet, the most abundant lipid consumed was TG 18:1_18:1_18:1 at 12.6 g/d. These…
Subjects/Keywords: macrolipidomics profiling; dietary food; MS/MS
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❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Yu, T. (2021). Developing Macrolipidomics Profiling Methods for Dietary Food Samples. (Thesis). University of Waterloo. Retrieved from http://hdl.handle.net/10012/16697
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Yu, Tianchunlu. “Developing Macrolipidomics Profiling Methods for Dietary Food Samples.” 2021. Thesis, University of Waterloo. Accessed April 18, 2021.
http://hdl.handle.net/10012/16697.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Yu, Tianchunlu. “Developing Macrolipidomics Profiling Methods for Dietary Food Samples.” 2021. Web. 18 Apr 2021.
Vancouver:
Yu T. Developing Macrolipidomics Profiling Methods for Dietary Food Samples. [Internet] [Thesis]. University of Waterloo; 2021. [cited 2021 Apr 18].
Available from: http://hdl.handle.net/10012/16697.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Yu T. Developing Macrolipidomics Profiling Methods for Dietary Food Samples. [Thesis]. University of Waterloo; 2021. Available from: http://hdl.handle.net/10012/16697
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
24.
Donato, Filipe Fagan.
Resíduos de agrotóxicos em água potável usando SPE e determinação rápida por LC-MS/MS e GC-MS/MS.
Degree: 2012, Universidade Federal de Santa Maria; Programa de Pós-Graduação em Química; UFSM; BR; Química
URL: http://repositorio.ufsm.br/handle/1/10528
► Conselho Nacional de Desenvolvimento Científico e Tecnológico
The use of pesticides always has been associated with the effective control of pests or invasive weeds to…
(more)
▼ Conselho Nacional de Desenvolvimento Científico e Tecnológico
The use of pesticides always has been associated with the effective control of pests or invasive weeds to ensure an increase in the food production. However, the indiscriminate use of these substances has caused the degradation of water resources. In Brazil, the Ministry of Health through Ordinance 2914 defines several parameters of potability, among them, the maximum limits allowed for same pesticides. In this work it was developed and validated a method for the determination of residues of 70 pesticides in drinking water using (SPE) for sample preparation and determination by Gas and Liquid Chromatography coupled to tandem mass spectrometry, triple quadrupole analyzer (GC-(TQ)MS/MS and LC- (TQ)MS/MS). It was evaluated different sample volume, sorbents and solvent of elution. The best results were obtained using 100 mL sample acidified at pH 2.5, Oasis® SPE cartridge HLB 60 mg/3 mL
and dichloromethane/methanol as eluent. Analytical curves were linear between 10 and 250 Sg L-1, with r2 values greater than 0.99 for all compounds. The values of method LOQ were 0.02 Sg L-1 for aldrin, dieldrin and chlordane and 0.5 Sg L-1 for the other compounds. To evaluate accuracy the blank samples ware fortified at 0.5, 1.5 and 4.0 Sg L- 1 and an extra level at 0.02 Sg L-1 for aldrin, dieldrin and chlordane. The method showed good precision, with RSD values below to 20% and good accuracy, with recoveries between 70 and 120%. Only the compounds methamidophos, aldicarb, benfuracarb, terbufos, benomyl and thiophanate methyl were not recovered adequately. The matrix effect was evaluated, showing upper 10% for the most compounds. In order to compensate this effect, analytical curves were obtained with standarts prepared in blank extracts of the matrix. The validated method was applied to 12 samples of drinking water of different characteristics (river, shed, well and treated water),
and just one of the river samples presented residues of lambda-cyhalothrin. The results indicate that the proposed method is suitable for analysis of pesticides residues in drinking water, since all the validation parameters met the suggested limits and parameters for validation of chromatographic methods.
O uso de agrotóxicos sempre esteve associado à efetividade no controle de pragas ou plantas invasoras para aumentar a produção de alimentos. No entanto, o uso indiscriminado dessas substâncias vem causando a degradação dos recursos hídricos. No Brasil, o Ministério da Saúde através da Portaria 2914 define diversos parâmetros de potabilidade, entre eles, os limites máximos permitidos para alguns agrotóxicos. No presente trabalho foi desenvolvido e validado um método para a determinação de resíduos de 70 agrotóxicos em água potável, utilizando Extração em Fase Sólida (SPE) para o preparo de amostra e determinação por Cromatografia Gasosa e Líquida, acopladas à Espectrometria de
Massas sequencial, com analisador triplo quadrupolo (GC-(TQ)MS/MS e LC- (TQ)MS/MS). Foram avaliados diferentes…
Advisors/Committee Members: Zanella, Renato, Prestes, Osmar Damian, Martins, Manoel Leonardo.
Subjects/Keywords: Agrotóxicos; SPE; Água; LC-MS/MS; GC-MS/MS; Pesticides; SPE; Water; LC-MS/MS; GC-MS/MS; CNPQ::CIENCIAS EXATAS E DA TERRA::QUIMICA
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APA (6th Edition):
Donato, F. F. (2012). Resíduos de agrotóxicos em água potável usando SPE e determinação rápida por LC-MS/MS e GC-MS/MS. (Masters Thesis). Universidade Federal de Santa Maria; Programa de Pós-Graduação em Química; UFSM; BR; Química. Retrieved from http://repositorio.ufsm.br/handle/1/10528
Chicago Manual of Style (16th Edition):
Donato, Filipe Fagan. “Resíduos de agrotóxicos em água potável usando SPE e determinação rápida por LC-MS/MS e GC-MS/MS.” 2012. Masters Thesis, Universidade Federal de Santa Maria; Programa de Pós-Graduação em Química; UFSM; BR; Química. Accessed April 18, 2021.
http://repositorio.ufsm.br/handle/1/10528.
MLA Handbook (7th Edition):
Donato, Filipe Fagan. “Resíduos de agrotóxicos em água potável usando SPE e determinação rápida por LC-MS/MS e GC-MS/MS.” 2012. Web. 18 Apr 2021.
Vancouver:
Donato FF. Resíduos de agrotóxicos em água potável usando SPE e determinação rápida por LC-MS/MS e GC-MS/MS. [Internet] [Masters thesis]. Universidade Federal de Santa Maria; Programa de Pós-Graduação em Química; UFSM; BR; Química; 2012. [cited 2021 Apr 18].
Available from: http://repositorio.ufsm.br/handle/1/10528.
Council of Science Editors:
Donato FF. Resíduos de agrotóxicos em água potável usando SPE e determinação rápida por LC-MS/MS e GC-MS/MS. [Masters Thesis]. Universidade Federal de Santa Maria; Programa de Pós-Graduação em Química; UFSM; BR; Química; 2012. Available from: http://repositorio.ufsm.br/handle/1/10528
Oregon State University
25.
Loewen, Matthew W.
Analysis of semi-volatile trace metals in basaltic glass by LA-ICP-MS.
Degree: MS, Geology, 2011, Oregon State University
URL: http://hdl.handle.net/1957/21822
► Semi-volatile trace metals (e.g., Cd, Sn, Pb, Zn, Cu, Mo) have been analyzed by using laser ablation ICP-MS in a number of silicate glasses (GSE-1G,…
(more)
▼ Semi-volatile trace metals (e.g., Cd, Sn, Pb, Zn, Cu, Mo) have been analyzed by using laser ablation ICP-
MS in a number of silicate glasses (GSE-1G, GSD-1G, NIST 610, NIST 612, BCR-2G, BHVO-2G). Our work investigates and highlights sources of uncertainty in the analysis of semi-volatile metals using LA-ICP-
MS. We identify within- cell transport fractionation as a primary source of uncertainty in these analyses. We found no evidence for significant fractionation of elements on the basis of volatility over typical 45 second ablation intervals. However, systematic fractionation of up to 20% was found for a number of siderophile and chalcophile trace elements as well as volatile lithophile elements when comparing analyses from different locations with a simple single-volume ablation chamber. Some fractionation was also observed for analyses in GSE-1G that intersected fractures or cracks in the glass, although overall GSE-1G appears to be homogeneous with respect to semi-volatile element distributions at the ~10 % level. Calibration using GSE-1G produces more accurate results on basaltic glass compositions than NIST 610. This work thus confirms the suitability of GSE-1G as a calibration standard for analysis of semi-volatile metals in mafic composition glasses.
This thesis also reports major and trace element data for glass, olivine, and olivine-hosted melt inclusions from the 1959 eruption of Kilauea Iki, Hawaii. Major element compositions of glasses match the results of earlier studies and suggest that dominant fractionation of olivine + Cr-spinel and secondary mixing between two magma batches control compositional variations. Melt inclusions trap melts with high sulfur concentrations (~0.1-0.15 wt.%) suggestive of a melt at or near sulfide saturation, while matrix glass is highly degassed. Lithophile trace element variations more clearly illustrate the effects of mixing at a late stage of magmatic development, and require magmas with two distinct mantle source regions and/or different degrees of partial melt. A number of non-traditional volatile trace elements were also analyzed including those with a range of volatility and geochemical affinity. Of these, most (e.g., Sn, Mo, Pb) display typical incompatible behavior while others appear to be compatible in known phases (primarily olivine: Zn, Co). Copper concentrations cannot be explained by removal of major phenocryst phases. Scatter in Cu concentrations could be achieved by either variable volatile mobility at depth or removal with a Cu-sulfide phase (either fractionated or residual). Previous studies of rare sulfides present in the 1959 eruption and more abundant sulfides crystallized in the lava lake suggest high Cu (>40 wt.%) in the sulfides. In this case even removal of a small amount of sulfide (<<1% by volume) could strongly deplete the melt of Cu while having little affect on other metals since their presence was not observed in the sulfides. There is no evidence for loss of volatile or semi-volatile trace metals during subaerial degassing.
Advisors/Committee Members: Kent, Adam (advisor), Dilles, John (committee member).
Subjects/Keywords: LA-ICP-MS
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APA ·
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APA (6th Edition):
Loewen, M. W. (2011). Analysis of semi-volatile trace metals in basaltic glass by LA-ICP-MS. (Masters Thesis). Oregon State University. Retrieved from http://hdl.handle.net/1957/21822
Chicago Manual of Style (16th Edition):
Loewen, Matthew W. “Analysis of semi-volatile trace metals in basaltic glass by LA-ICP-MS.” 2011. Masters Thesis, Oregon State University. Accessed April 18, 2021.
http://hdl.handle.net/1957/21822.
MLA Handbook (7th Edition):
Loewen, Matthew W. “Analysis of semi-volatile trace metals in basaltic glass by LA-ICP-MS.” 2011. Web. 18 Apr 2021.
Vancouver:
Loewen MW. Analysis of semi-volatile trace metals in basaltic glass by LA-ICP-MS. [Internet] [Masters thesis]. Oregon State University; 2011. [cited 2021 Apr 18].
Available from: http://hdl.handle.net/1957/21822.
Council of Science Editors:
Loewen MW. Analysis of semi-volatile trace metals in basaltic glass by LA-ICP-MS. [Masters Thesis]. Oregon State University; 2011. Available from: http://hdl.handle.net/1957/21822
University of Alberta
26.
McKnight-Whitford, Anthony Nicholai.
Arsenic Binding to Thiols and Applications to Electrospray
Mass Spectrometry Detection.
Degree: PhD, Department of Chemistry, 2010, University of Alberta
URL: https://era.library.ualberta.ca/files/rf55z9287
► Arsenic is a widespread environmental contaminant whose toxicity depends on its valence and its chemical form. Arsenic species have been typically determined using high pressure…
(more)
▼ Arsenic is a widespread environmental contaminant
whose toxicity depends on its valence and its chemical form.
Arsenic species have been typically determined using high pressure
liquid chromatography coupled to inductively coupled plasma mass
spectrometry (HPLC-ICPMS), however ICPMS cannot differentiate the
co-eluting arsenic species. This thesis explores the use of
electrospray mass spectrometry (ESI-MS) with HPLC separation for
arsenic speciation and demonstrates applications of various
HPLC-ESI-MS methods for the determination of toxicologically and
environmentally relevant arsenic compounds. The trivalent
arsenicals, such as arsenite (AsIII) and its metabolites
monomethylarsonous acid (MMAIII) and dimethylarsinous acid (DMAIII)
are not easily detected using ESI-MS due to their poor
ionizability, but they are known to have high affinity for thiols.
Thus, the easily ionizable dithiol dimercaptosuccinic acid (DMSA)
was used to derivatize the trivalent arsenicals prior to ESI-MS.
Selection of the derivatizing reaction was based on studies of
arsenic-thiol interactions. An HPLC-ESI-MS/MS method was developed
for the detection of derivatized AsIII, DMAIII and MMAIII and
underivatized arsenate (AsV), monomethylarsonic acid (MMAV) and
dimethylarsinic acid (DMAV), and was used to analyze multiple types
of samples including urine, plasma and water. One set of
groundwater samples from the site of a former pesticide
manufacturing plant contained concentrations of MMAIII as high as
3.9-274 mg/L, the highest ever observed in the environment. Another
HPLC-ESI-MS/MS method, without the need of derivatization, was
developed for the detection of the toxic thio-arsenicals
dimethylmonothioarsinic acid (DMMTAV) and monomethylmonothioarsonic
acid (MMMTAV). DMMTAV was present in rat plasma and human urine and
both DMMTAV and MMMTAV were detected in rat urine. The method of
derivatization and ESI-MS/MS detection was extended to the
speciation of inorganic SbIII and SbV. The use of the
HPLC-ESI-MS/MS method using DMPS derivatization enabled the
speciation of SbIII and SbV in water samples from mine
waste.
Subjects/Keywords: Arsenic; ESI-MS
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APA (6th Edition):
McKnight-Whitford, A. N. (2010). Arsenic Binding to Thiols and Applications to Electrospray
Mass Spectrometry Detection. (Doctoral Dissertation). University of Alberta. Retrieved from https://era.library.ualberta.ca/files/rf55z9287
Chicago Manual of Style (16th Edition):
McKnight-Whitford, Anthony Nicholai. “Arsenic Binding to Thiols and Applications to Electrospray
Mass Spectrometry Detection.” 2010. Doctoral Dissertation, University of Alberta. Accessed April 18, 2021.
https://era.library.ualberta.ca/files/rf55z9287.
MLA Handbook (7th Edition):
McKnight-Whitford, Anthony Nicholai. “Arsenic Binding to Thiols and Applications to Electrospray
Mass Spectrometry Detection.” 2010. Web. 18 Apr 2021.
Vancouver:
McKnight-Whitford AN. Arsenic Binding to Thiols and Applications to Electrospray
Mass Spectrometry Detection. [Internet] [Doctoral dissertation]. University of Alberta; 2010. [cited 2021 Apr 18].
Available from: https://era.library.ualberta.ca/files/rf55z9287.
Council of Science Editors:
McKnight-Whitford AN. Arsenic Binding to Thiols and Applications to Electrospray
Mass Spectrometry Detection. [Doctoral Dissertation]. University of Alberta; 2010. Available from: https://era.library.ualberta.ca/files/rf55z9287
Universidade Federal de Santa Maria
27.
Magali Kemmerich.
DETERMINAÇÃO MUTIRRESÍDUO DE AGROTÓXICOS EM PIMENTÃO UTILIZANDO MÉTODO QuEChERS MODIFICADO E UHPLC-MS/MS.
Degree: 2013, Universidade Federal de Santa Maria
URL: http://coralx.ufsm.br/tede/tde_busca/arquivo.php?codArquivo=5212
► Nos últimos anos, o uso indiscriminado de agrotóxicos e a não adoção das boas práticas agrícolas têm sido evidenciados pelos resultados de análises de resíduos…
(more)
▼ Nos últimos anos, o uso indiscriminado de agrotóxicos e a não adoção das boas práticas agrícolas têm sido evidenciados pelos resultados de análises de resíduos de agrotóxicos em alimentos, como o pimentão, um dos recordistas brasileiros com princípios ativos proibidos ou acima dos limites recomendados. Assim, o presente trabalho teve como objetivo desenvolver e validar um método rápido para a determinação de 83 agrotóxicos em pimentão, utilizando método QuEChERS modificado e Cromatografia Líquida de Ultra Eficiência acoplada à Espectrometria de Massas em série (UHPLC-MS/MS). Na etapa de preparo de amostra testou-se procedimentos de extração/partição, além de diferentes sorventes para a etapa de limpeza, como terra diatomácea e sorvente polimérico. Utilizou-se o planejamento fatorial 24 para otimização do método de extração, o qual foi constituído da extração de 10,0 g de pimentão com 10,0 mL de acetonitrila. Na etapa de partição adicionou-se 1,5 g de NaCl e 4,0 g de MgSO4, seguindo-se agitação e centrifugação. Realizou-se a etapa de limpeza de 1,0 mL do extrato com 40,0 mg de PSA e 150,0 mg de MgSO4, seguido de agitação e centrifugação. O extrato foi filtrado, diluído e analisado por UHPLC-MS/MS. A maioria dos compostos apresentou faixa linear (r2≥ 0,99). O efeito matriz foi compensado utilizando-se curvas analíticas preparadas no extrato branco da matriz. A exatidão do método foi verificada com ensaios de recuperação em quatro níveis de concentração, obtendose valores entre 70,0 e 120,0% para a maioria dos compostos avaliados. Valores de LODm e LOQm foram de 3 ou 7,5 μg kg-1 e 10 ou 25 μg kg-1, respetivamente.Valores de RSD inferiores a 20% garantiram a boa precisão do método. A aplicação do método foi realizada em 20 amostras de pimentão, nas quais resíduos de acetamiprido, azoxistrobina, boscalida, carbendazim, clorpirifós etílico, clotianidina, difenoconazol, fempropatrina, piraclostrobina e pirimetanil foram quantificados, em concentrações que variaram de 10 a 294 μg kg-1. O método QuEChERS modificado proposto para a determinação de resíduos de 81 dos 83 agrotóxicos em pimentão utilizando UHPLC-MS/MS mostrou ser eficaz, podendo ser aplicado em análises de rotina.
In recent years, the indiscriminate use of pesticides and non-adoption of good agricultural practices have been evidenced by the results of analysis of pesticide residues in food, such as sweet pepper, one of Brazilian record holders with active ingredients that are banned or above the recommended limits. Thus, the present study aimed to develop and validate a rapid method for the determination of 83 pesticides in sweet pepper using modified QuEChERS method and Ultra-high Performance Liquid Chromatography coupled to tandem Mass Spectrometry (UHPLC-MS/MS). In the sample preparation extraction/partition procedures, and different sorbents for clean-up were tested, such as diatomaceous earth and polymeric sorbent. It was used the 24 factorial design for optimization of the method of extraction, which consisted of the extraction of…
Advisors/Committee Members: Renato Zanella, Osmar Damian Prestes, Liane Maldaner.
Subjects/Keywords: agrotóxicos; pimentão; UHPLC-MS/MS; QuEChERS; QUIMICA; UHPLC-MS/MS; QuEChERS; sweet pepper; pesticides
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APA ·
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APA (6th Edition):
Kemmerich, M. (2013). DETERMINAÇÃO MUTIRRESÍDUO DE AGROTÓXICOS EM PIMENTÃO UTILIZANDO MÉTODO QuEChERS MODIFICADO E UHPLC-MS/MS. (Thesis). Universidade Federal de Santa Maria. Retrieved from http://coralx.ufsm.br/tede/tde_busca/arquivo.php?codArquivo=5212
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Kemmerich, Magali. “DETERMINAÇÃO MUTIRRESÍDUO DE AGROTÓXICOS EM PIMENTÃO UTILIZANDO MÉTODO QuEChERS MODIFICADO E UHPLC-MS/MS.” 2013. Thesis, Universidade Federal de Santa Maria. Accessed April 18, 2021.
http://coralx.ufsm.br/tede/tde_busca/arquivo.php?codArquivo=5212.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Kemmerich, Magali. “DETERMINAÇÃO MUTIRRESÍDUO DE AGROTÓXICOS EM PIMENTÃO UTILIZANDO MÉTODO QuEChERS MODIFICADO E UHPLC-MS/MS.” 2013. Web. 18 Apr 2021.
Vancouver:
Kemmerich M. DETERMINAÇÃO MUTIRRESÍDUO DE AGROTÓXICOS EM PIMENTÃO UTILIZANDO MÉTODO QuEChERS MODIFICADO E UHPLC-MS/MS. [Internet] [Thesis]. Universidade Federal de Santa Maria; 2013. [cited 2021 Apr 18].
Available from: http://coralx.ufsm.br/tede/tde_busca/arquivo.php?codArquivo=5212.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Kemmerich M. DETERMINAÇÃO MUTIRRESÍDUO DE AGROTÓXICOS EM PIMENTÃO UTILIZANDO MÉTODO QuEChERS MODIFICADO E UHPLC-MS/MS. [Thesis]. Universidade Federal de Santa Maria; 2013. Available from: http://coralx.ufsm.br/tede/tde_busca/arquivo.php?codArquivo=5212
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Universidade Federal de Santa Maria
28.
Mateus Brum Pereira.
AVALIAÇÃO DA TÉCNICA DE EXTRAÇÃO DE SPE E GC-(EI)-MS/MS NA ANÁLISE DE AGROTÓXICOS EM ÁGUA DE LAVOURA DE ARROZ IRRIGADO.
Degree: 2012, Universidade Federal de Santa Maria
URL: http://coralx.ufsm.br/tede/tde_busca/arquivo.php?codArquivo=4488
► O uso intensivo de agrotóxicos, especialmente herbicidas e inseticidas, associados ao manejo inadequado da água de irrigação pode resultar no escoamento destes compostos para os…
(more)
▼ O uso intensivo de agrotóxicos, especialmente herbicidas e inseticidas, associados ao manejo inadequado da água de irrigação pode resultar no escoamento destes compostos para os rios e lagoas. Ressalta-se que, o cultivo de arroz é feito preferencialmente em áreas que possuem grandes volumes de água disponíveis. Considerando-se a importância da água para a natureza e para a saúde humana, bem como, a escassez dos recursos hídricos e a produção em larga escala de arroz indispensável como alimento para a população, este estudo tem como objetivo desenvolver e validar um método para a determinação de 62 agrotóxicos em água de lavoura de arroz irrigado. Num primeiro momento, os analitos foram extraídos das amostras utilizando o método de extração em fase sólida (SPE) e a determinação simultânea dos agrotóxicos selecionados foi realizada por um GC-(EI) - MS/MS. Em seguida, para cada composto, duas transições MRM foram monitoradas: uma para quantificação e outra para confirmação, como o previsto pelas diretrizes internacionais e legislações. Em continuidade, na validação do método, os resultados foram satisfatórios para os requisitos exigidos, sendo que os valores de r para as curvas analíticas foram maiores que 0,99. O valor do LOQ do método para todos os compostos foi estabelecido em 0,2 μg L-1. As recuperações apresentaram valores entre 70 e 120% com RSD inferior a 20% para a maioria dos compostos estudados. Finalmente, os resultados indicam que o método proposto para a análise de 32 agrotóxicos - incluindo uma etapa de extração e quantificação por SPE-GC- (EI) -MS/MS - é eficiente e seletiva, permitindo a quantificação de baixos níveis de concentração. Esses índices tornam a técnica adequada para o monitoramento destes compostos em água de lavoura de arroz irrigado. Além disso, os parâmetros de validação atendem aos requisitos mínimos para a validação de métodos cromatográficos, resultado que permitiu a aplicação do método desenvolvido e validado.
It is known that heavy use of pesticides, especially herbicides and insecticides, associated with the improper management of irrigation water can result in pesticide runoff out the crops to the waters streams, rivers or ponds, since the cultivation of rice is preferably done in areas that have large volumes of water available. Considering the importance of water for nature and human health, the low uncontaminated water resources available today and a large scale production of rice, this study aimed to develop and validate a method for the determination of pesticides in water from irrigated rice crop. Pesticides were extracted from samples of river water using the method of solid phase extraction (SPE). The simultaneous determination of selected pesticides in this study was made by a GC-(EI)-MS/MS. For each analyte, two MRM transitions were optimized, one for quantification and one for confirmation, as predicted by international guidelines and legislations. In the method validation the results were satisfactory for validation requirements, and the analytical curves of r…
Advisors/Committee Members: Martha Bohrer Adaime, Manoel Leonardo Martins, Fabio Ferreira Gonçalves.
Subjects/Keywords: GC-(EI)-MS/MS; água; agrotóxicos; QUIMICA; pesticides; water; GC-(EI)-MS/MS
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APA ·
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MLA ·
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CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Pereira, M. B. (2012). AVALIAÇÃO DA TÉCNICA DE EXTRAÇÃO DE SPE E GC-(EI)-MS/MS NA ANÁLISE DE AGROTÓXICOS EM ÁGUA DE LAVOURA DE ARROZ IRRIGADO. (Thesis). Universidade Federal de Santa Maria. Retrieved from http://coralx.ufsm.br/tede/tde_busca/arquivo.php?codArquivo=4488
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Pereira, Mateus Brum. “AVALIAÇÃO DA TÉCNICA DE EXTRAÇÃO DE SPE E GC-(EI)-MS/MS NA ANÁLISE DE AGROTÓXICOS EM ÁGUA DE LAVOURA DE ARROZ IRRIGADO.” 2012. Thesis, Universidade Federal de Santa Maria. Accessed April 18, 2021.
http://coralx.ufsm.br/tede/tde_busca/arquivo.php?codArquivo=4488.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Pereira, Mateus Brum. “AVALIAÇÃO DA TÉCNICA DE EXTRAÇÃO DE SPE E GC-(EI)-MS/MS NA ANÁLISE DE AGROTÓXICOS EM ÁGUA DE LAVOURA DE ARROZ IRRIGADO.” 2012. Web. 18 Apr 2021.
Vancouver:
Pereira MB. AVALIAÇÃO DA TÉCNICA DE EXTRAÇÃO DE SPE E GC-(EI)-MS/MS NA ANÁLISE DE AGROTÓXICOS EM ÁGUA DE LAVOURA DE ARROZ IRRIGADO. [Internet] [Thesis]. Universidade Federal de Santa Maria; 2012. [cited 2021 Apr 18].
Available from: http://coralx.ufsm.br/tede/tde_busca/arquivo.php?codArquivo=4488.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Pereira MB. AVALIAÇÃO DA TÉCNICA DE EXTRAÇÃO DE SPE E GC-(EI)-MS/MS NA ANÁLISE DE AGROTÓXICOS EM ÁGUA DE LAVOURA DE ARROZ IRRIGADO. [Thesis]. Universidade Federal de Santa Maria; 2012. Available from: http://coralx.ufsm.br/tede/tde_busca/arquivo.php?codArquivo=4488
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Universidade Federal de Santa Maria
29.
Danieli Daiani Bandeira.
DETERMINAÇÃO DE RESÍDUOS DE AGROTÓXICOS NO LEITE BOVINO EMPREGANDO MÉTODO QuEChERS MODIFICADO E GC-MS/MS.
Degree: 2012, Universidade Federal de Santa Maria
URL: http://coralx.ufsm.br/tede/tde_busca/arquivo.php?codArquivo=4496
► For providing a balanced composition, among fresh food, the milk is considered one of the most nutritionally complete, being a product very well accepted in…
(more)
▼ For providing a balanced composition, among fresh food, the milk is considered one of the most nutritionally complete, being a product very well accepted in the market with consumers of all ages. Thus, the analysis of milk quality is great importance for consumer information and verification of the parameters required by law. Considering all the problems involving the many chemicals used for several purposes in agriculture, and the possibility of these compounds directly or indirectly the milk and cause damage to population health, this work aimed to develope and validate a method for a multiresidue determination of 48 pesticides in cattle milk, using modified QuEChERS method and GC-MS/MS. The extraction procedure consisted of 5.0 mL of sample (milk) extracted with 5.0 mL of acetonitrile, acidified with 1% of acetic acid (v/v), followed by manual shaking for 1 min for partition step was used 2.0 g of anhydrous MgSO4 and 0.85 g of anhydrous sodium acetate. Manual agitation was repeated for 1 min, and subsequently it was performed centrifugation at 3400 rpm for 8 min. The dispersive clean up step consisted in add 1.0 mL of supernatant extract, 150.0 mg of anhydrous MgSO4, 125.0 mg of C18 and 62.0 mg of PSA in an eppendorf tube, which was shaked in a vortex for 1 min, centrifuged at 10000 rpm for 3 min. After centrifugation, the extract was transferred to a glass insert for the injection into the GC-MS/MS system. It was evaluated the linearity, limits of detection (LOD) and quantitation (LOQ), the matrix effect, accuracy and precision of the analytical method. The method proved to be efficient, with satisfactory results for recoveries, with values in a range between 71.1 and 117.4% for 45 of the 48 compounds analyzed. RSD values lowerd than 17,3%, values of LODi and LODm ranged between 0.3 to 3.0 μg L-1 and LOQi and LOQm ranged between 1.0 to 10.0 μg L-1. The validated method was applied for the determination of residues of 45 pesticides/degradation products in samples of milk UHT. The method developed was adequate for the analysis of pesticide residues in cattle milk, as all the validation parameters were within the limits required for validation of chromatographic methods, achieving satisfactorily the objectives outlined in this paper.
Por oferecer uma composição equilibrada, o leite é considerado um dos alimentos in natura mais completos nutricionalmente, sendo um produto muito bem aceito no mercado e tendo consumidores de todas as idades. Sendo assim, a análise da qualidade do leite é de grande importância para informação do consumidor e verificação dos parâmetros exigidos pela legislação. Considerando toda a problemática envolvendo as inúmeras substâncias químicas utilizadas com finalidades diversas na agropecuária, e a possibilidade destas chegarem direta ou indiretamente até o leite e causarem danos a saúde da população, neste trabalho foi desenvolvido e validado um método multirresíduo para a determinação de 48 agrotóxicos em leite de vaca, empregando método QuEChERS modificado e GCMS/ MS. O…
Advisors/Committee Members: Martha Bohrer Adaime, Neila Silvia Pereira dos Santos Richards, Osmar Damian Prestes.
Subjects/Keywords: pesticide; milk; modified QuEChERS; GC-MS/MS; QUIMICA; GC-MS/MS; QuEChERS modificado; agrotóxicos; leite
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APA (6th Edition):
Bandeira, D. D. (2012). DETERMINAÇÃO DE RESÍDUOS DE AGROTÓXICOS NO LEITE BOVINO EMPREGANDO MÉTODO QuEChERS MODIFICADO E GC-MS/MS. (Thesis). Universidade Federal de Santa Maria. Retrieved from http://coralx.ufsm.br/tede/tde_busca/arquivo.php?codArquivo=4496
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Bandeira, Danieli Daiani. “DETERMINAÇÃO DE RESÍDUOS DE AGROTÓXICOS NO LEITE BOVINO EMPREGANDO MÉTODO QuEChERS MODIFICADO E GC-MS/MS.” 2012. Thesis, Universidade Federal de Santa Maria. Accessed April 18, 2021.
http://coralx.ufsm.br/tede/tde_busca/arquivo.php?codArquivo=4496.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Bandeira, Danieli Daiani. “DETERMINAÇÃO DE RESÍDUOS DE AGROTÓXICOS NO LEITE BOVINO EMPREGANDO MÉTODO QuEChERS MODIFICADO E GC-MS/MS.” 2012. Web. 18 Apr 2021.
Vancouver:
Bandeira DD. DETERMINAÇÃO DE RESÍDUOS DE AGROTÓXICOS NO LEITE BOVINO EMPREGANDO MÉTODO QuEChERS MODIFICADO E GC-MS/MS. [Internet] [Thesis]. Universidade Federal de Santa Maria; 2012. [cited 2021 Apr 18].
Available from: http://coralx.ufsm.br/tede/tde_busca/arquivo.php?codArquivo=4496.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Bandeira DD. DETERMINAÇÃO DE RESÍDUOS DE AGROTÓXICOS NO LEITE BOVINO EMPREGANDO MÉTODO QuEChERS MODIFICADO E GC-MS/MS. [Thesis]. Universidade Federal de Santa Maria; 2012. Available from: http://coralx.ufsm.br/tede/tde_busca/arquivo.php?codArquivo=4496
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Universidade Federal de Santa Maria
30.
Osmar Damian Prestes.
MÉTODO RÁPIDO PARA A DETERMINAÇÃO SIMULTÂNEA DE RESÍDUOS DE AGROTÓXICOS E MEDICAMENTOS VETERINÁRIOS EM ALIMENTOS DE ORIGEM ANIMAL POR LC-MS/MS.
Degree: 2011, Universidade Federal de Santa Maria
URL: http://coralx.ufsm.br/tede/tde_busca/arquivo.php?codArquivo=3597
► Atualmente, uma das maiores barreiras comerciais que o Brasil tem encontrado para a comercialização de seus produtos da cadeia do agronegócio é a falta de…
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▼ Atualmente, uma das maiores barreiras comerciais que o Brasil tem encontrado para a comercialização de seus produtos da cadeia do agronegócio é a falta de informações sobre a presença de resíduos em alimentos produzidos no país. Devido a complexidade das matrizes de origem animal e das baixas concentrações dos agrotóxicos e medicamentos veterinários presentes, há uma grande necessidade de desenvolvimento de métodos analíticos eficientes e confiáveis para a identificação e quantificação dos resíduos. Neste estudo avaliou-se a faixa de linearidade das curvas analíticas (8 níveis de concentração e 6 injeções cada), limite de detecção (LOD), limite de quantificação (LOQ), efeito matriz, bem como precisão e exatidão, em termos de percentual de recuperação, para 91 agrotóxicos e 9 medicamentos veterinários analisados e validados pelo método QuEChERS e Cromatografia Líquida Acoplada à Espectrometria de Massas Sequencial em amostras de carne, fígado, rim, leite e ovo. Para isso, efetuou-se a fortificação dos alimentos previamente homogeinizados, com soluções contendo os 100 compostos, em 3 níveis de fortificação (10, 25 e 50 μg kg-1), 6 réplicas para cada nível, e aplicou-se o método de extração. A extração pelo método QuEChERS modificado consistiu na pesagem de 10,0 g da matriz (para leite 10 mL), 10 mL de acetonitrila contendo 1% (v/v) de ácido acético e procedeu-se a agitação manual e vigorosa, por cerca de 1 min. Acrescentou-se 4,0 g de sulfato de magnésio anidro e 1,7 g de acetato de sódio anidro, repetindo-se a agitação. Foram, posteriormente, centrifugados por 8 min (3500 rpm), após 4 mL de extrato foram transferidos para outro tubo contendo 600 mg de sulfato de magnésio anidro e 500 mg C18, repetindo a agitação e a centrifugação. O extrato foi diluído (1:1) na fase móvel, e em seguida analisado por LC-MS/MS. As curvas analíticas, preparadas no solvente e nos extratos das matrizes avaliadas, apresentaram linearidade adequada entre 1,0 e 250,0 g L-1, com valores de coeficiente de determinação maiores que 0,995. O método apresentou valores de recuperação satisfatórios entre 70 e 120% e (RSD ≤ 20%) para cerca de 85% dos compostos em todas as matrizes nos níveis de 10, 25 e 50 μg kg-1 (para leite μg L-1). Em geral, o método apresentou valor de LOQm de 10 μg kg-1 (μg L-1). Portanto, concluiu-se que o método proposto pode ser aplicado de forma eficiente para a determinação de resíduos de agrotóxicos e medicamentos veterinários nas diferentes matrizes avaliadas, sem a necessida de alteração do método desenvolvido foi eficiente para a extração e clean-up dos extratos de carne, fígado, rim, leite e ovo, uma vez que apresentou desempenho satisfatório em ambas as matrizes, dependendo da técnica de detecção utilizada.
Currently, one of highest trade barriers that Brazil has found for the marketing of their products from the agribusiness chain is the lack of informations on the presence of residues in foods produced in the country. Due to the complexity of the matrices of animal origin and the low…
Advisors/Committee Members: Renato Zanella, Martha Bohrer Adaime, Roger Wagner, Isabel Cristina Sales Fontes Jardim, Carol Hollingworth Collins.
Subjects/Keywords: medicamentos veterinários; agrotóxicos; LC-MS/MS; QuEChERS; QUIMICA; QuEChERS; LC-MS/MS; pesticides; veterinary drugs
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Record Details
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APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Prestes, O. D. (2011). MÉTODO RÁPIDO PARA A DETERMINAÇÃO SIMULTÂNEA DE RESÍDUOS DE AGROTÓXICOS E MEDICAMENTOS VETERINÁRIOS EM ALIMENTOS DE ORIGEM ANIMAL POR LC-MS/MS. (Thesis). Universidade Federal de Santa Maria. Retrieved from http://coralx.ufsm.br/tede/tde_busca/arquivo.php?codArquivo=3597
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Prestes, Osmar Damian. “MÉTODO RÁPIDO PARA A DETERMINAÇÃO SIMULTÂNEA DE RESÍDUOS DE AGROTÓXICOS E MEDICAMENTOS VETERINÁRIOS EM ALIMENTOS DE ORIGEM ANIMAL POR LC-MS/MS.” 2011. Thesis, Universidade Federal de Santa Maria. Accessed April 18, 2021.
http://coralx.ufsm.br/tede/tde_busca/arquivo.php?codArquivo=3597.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Prestes, Osmar Damian. “MÉTODO RÁPIDO PARA A DETERMINAÇÃO SIMULTÂNEA DE RESÍDUOS DE AGROTÓXICOS E MEDICAMENTOS VETERINÁRIOS EM ALIMENTOS DE ORIGEM ANIMAL POR LC-MS/MS.” 2011. Web. 18 Apr 2021.
Vancouver:
Prestes OD. MÉTODO RÁPIDO PARA A DETERMINAÇÃO SIMULTÂNEA DE RESÍDUOS DE AGROTÓXICOS E MEDICAMENTOS VETERINÁRIOS EM ALIMENTOS DE ORIGEM ANIMAL POR LC-MS/MS. [Internet] [Thesis]. Universidade Federal de Santa Maria; 2011. [cited 2021 Apr 18].
Available from: http://coralx.ufsm.br/tede/tde_busca/arquivo.php?codArquivo=3597.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Prestes OD. MÉTODO RÁPIDO PARA A DETERMINAÇÃO SIMULTÂNEA DE RESÍDUOS DE AGROTÓXICOS E MEDICAMENTOS VETERINÁRIOS EM ALIMENTOS DE ORIGEM ANIMAL POR LC-MS/MS. [Thesis]. Universidade Federal de Santa Maria; 2011. Available from: http://coralx.ufsm.br/tede/tde_busca/arquivo.php?codArquivo=3597
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
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