subject:(MMP inhibition)

1. Boräng, Jennifer. Green tea inhibits proteolytic enzymes in GCF from patients with chronic periodontitis.

Degree: 2012, , Faculty of Odontology (OD)

URL: http://urn.kb.se/resolve?urn=urn:nbn:se:mau:diva-19944

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Kronisk parodontit orsakar vävnadsdestruktion till följd av matrixmetalloproteinasaktivitet. Dessa enzym härrör från värdcellerna och är en del av det immunologiska svaret på bakteriella virulensfaktorer.… (more)

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Kronisk parodontit orsakar vävnadsdestruktion till följd av matrixmetalloproteinasaktivitet. Dessa enzym härrör från värdcellerna och är en del av det immunologiska svaret på bakteriella virulensfaktorer. Grönt te har studerats för sina hälsofrämjande egenskaper, som omfattar bland annat anti-inflammatoriska effekter. Effekten beror delvis på enzyminhibering av tepolyfenoler. Syftet med denna studie var att ytterligare undersöka den inhiberande effekten av grönt te, med fokus på enzymatisk aktivitet i gingivalvätska från patienter med parodontal sjukdom. Patienter med kronisk parodontit valdes ut för att delta i studien. Gingivalvätska extraherades med mikropipetter från patienternas gingivala sulci. Proverna behandlades med grönt te och jämfördes med obehandlade prover från samma försöksperson. Fluorescens proteasanalys med kasein som substrat utfördes på fjorton prover för att detektera skillnader i kaseinolytisk aktivitet. Zymogramanalys med användning av gelatin som substrat utfördes på fyra prover, för att undersöka skillnader i gelatinolytisk aktivitet och analysera molekylvikter för de olika enzymerna. Den fluorometriska analysen visade en signifikant lägre enzymaktivitet i prover med tillsatt grönt te jämfört med obehandlade prover (p<0.001). Zymogramanalysen visade en skillnad i enzymaktivitet som var mest uttalad i banden för molekylär vikt runt 255 kDa, analogt med komplex av matrixmetalloproteinas-9. Sammanfattningsvis har det i denna studie påvisats att grönt te har en hämmande effekt på kaseinolytisk aktivitet och en mindre, mer specifik, hämmande effekt på gelatinasaktivitet.

Chronic periodontitis involves tissue destruction by matrix metalloproteinase, derived from the host cells, as part of the immunological response to bacterial virulence factors. Green tea has been studied for its health promoting properties, which includes anti-inflammatory effects. The effect is in part due to enzyme inhibition by tea polyphenols. The aim of this study was to further investigate the inhibitory effect of green tea, focusing on enzymatic activity in gingival crevicular fluid from patients with periodontal disease. Patients with chronic periodontitis were selected for participation in the study. Gingival crevicular fluid was extracted with micropipettes from the gingival sulci of the patients. Samples were treated with green tea and compared with untreated samples from the same subject. Fluorescence protease assay with casein as substrate was made using fourteen samples for detecting differences in caseinolytic activity. Zymogram assay using gelatin as substrate was done using four samples to test gelatinolytic activity and analyse molecular weights of the different enzymes. The fluorometric assay showed a significantly lower enzyme activity in samples mixed with green tea than untreated samples (p<0.001). The zymogram assay showed a difference in band strength which was most pronounced in the bands of molecular weight around 255 kDA, analogous to complexes of matrix metalloproteinase-9. In conclusion,…

Subjects/Keywords: green tea; polyphenols; EGCg; GCF; gingival crevicular fluid; periodontitis; MMP; inhibition; enzyme; fluorescence assay; zymography; Medical and Health Sciences; Medicin och hälsovetenskap

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APA (6^th Edition):

Boräng, J. (2012). Green tea inhibits proteolytic enzymes in GCF from patients with chronic periodontitis. (Thesis). , Faculty of Odontology (OD). Retrieved from http://urn.kb.se/resolve?urn=urn:nbn:se:mau:diva-19944

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Boräng, Jennifer. “Green tea inhibits proteolytic enzymes in GCF from patients with chronic periodontitis.” 2012. Thesis, , Faculty of Odontology (OD). Accessed March 08, 2021. http://urn.kb.se/resolve?urn=urn:nbn:se:mau:diva-19944.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Boräng, Jennifer. “Green tea inhibits proteolytic enzymes in GCF from patients with chronic periodontitis.” 2012. Web. 08 Mar 2021.

Vancouver:

Boräng J. Green tea inhibits proteolytic enzymes in GCF from patients with chronic periodontitis. [Internet] [Thesis]. , Faculty of Odontology (OD); 2012. [cited 2021 Mar 08]. Available from: http://urn.kb.se/resolve?urn=urn:nbn:se:mau:diva-19944.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Boräng J. Green tea inhibits proteolytic enzymes in GCF from patients with chronic periodontitis. [Thesis]. , Faculty of Odontology (OD); 2012. Available from: http://urn.kb.se/resolve?urn=urn:nbn:se:mau:diva-19944

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Louisiana State University

2. Fugler, Lee Ann. Matrix metalloproteinases in the equine systemic inflammatory response: implications for equine laminitis.

Degree: PhD, Veterinary Medicine, 2008, Louisiana State University

URL: etd-01212009-182854 ; https://digitalcommons.lsu.edu/gradschool_dissertations/908

► Laminitis is a crippling and often life-threatening disease of the equine foot. Soft tissue damage characteristic of this disease has been associated with increased MMP… (more)

▼ Laminitis is a crippling and often life-threatening disease of the equine foot. Soft tissue damage characteristic of this disease has been associated with increased MMP activity. Therefore, it seems likely that MMPIs could be potential therapeutic agents for laminitis. Further characterization of equine MMPs and evaluation of the effectiveness of MMPIs in the horse are needed. Equine MMP-9 was harvested from neutrophils, purified by affinity chromatography, and evaluated using western blotting and gelatin zymography. The Biotrak MMP-9 Activity Assay was evaluated for use with equine samples using equine neutrophil MMP-9 as a standard, and was determined to have insufficient sensitivity for equine MMP-9. Therefore, zymography was used for evaluating MMP activity in all studies. The abilities of doxycycline, oxytetracycline, and flunixin meglumine to inhibit LPS-induced equine MMP-2 and MMP-9 activities in vitro were investigated using a digital laminar explant model. The structural integrity of the explants was tested using an Instron biomechanical testing device, and MMP activity in the explants medium was evaluated using zymography. Doxycycline, oxytetracycline, and flunixin meglumine all successfully inhibited equine MMP-9 to varying degrees. However, only doxycycline and oxytetracycline increased the structural integrity of the explants. Explant structural integrity was inversely correlated with MMP-2 concentrations in the medium. Based on the in vitro results, a non-terminal in vivo model for investigating MMPIs in the horse was validated. The administration of IV endotoxin to normal adult horses resulted in significant increases in MMP-2 and MMP-9 activities, as assessed by zymography. This in vivo model of MMP induction was used to determine the effects of doxycycline, oxytetracycline, flunixin meglumine, and pentoxifylline on equine MMP inhibition. Pentoxifylline and oxytetracycline appeared to be potent MMP-9 and modest MMP-2 inhibitors in the horse. Flunixin meglumine and doxycycline were potent inhibitors of equine MMP-2, but only weak inhibitors of equine MMP-9. These findings warrant the evaluation of pentoxifylline and oxytetracycline as MMPIs in the prevention/treatment of equine laminitis.

Subjects/Keywords: equine; horse; matrix metalloproteinase; MMP; endotoxin; laminitis; doxycycline; pentoxifylline; oxytetracycline; flunixin meglumine; MMP inhibition; endotoxemia; experimental endotoxemia; systemic inflammatory response; SIRS; laminae; laminar explants; biomechanical testing; zymography

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Fugler, L. A. (2008). Matrix metalloproteinases in the equine systemic inflammatory response: implications for equine laminitis. (Doctoral Dissertation). Louisiana State University. Retrieved from etd-01212009-182854 ; https://digitalcommons.lsu.edu/gradschool_dissertations/908

Chicago Manual of Style (16^th Edition):

Fugler, Lee Ann. “Matrix metalloproteinases in the equine systemic inflammatory response: implications for equine laminitis.” 2008. Doctoral Dissertation, Louisiana State University. Accessed March 08, 2021. etd-01212009-182854 ; https://digitalcommons.lsu.edu/gradschool_dissertations/908.

MLA Handbook (7^th Edition):

Fugler, Lee Ann. “Matrix metalloproteinases in the equine systemic inflammatory response: implications for equine laminitis.” 2008. Web. 08 Mar 2021.

Vancouver:

Fugler LA. Matrix metalloproteinases in the equine systemic inflammatory response: implications for equine laminitis. [Internet] [Doctoral dissertation]. Louisiana State University; 2008. [cited 2021 Mar 08]. Available from: etd-01212009-182854 ; https://digitalcommons.lsu.edu/gradschool_dissertations/908.

Council of Science Editors:

Fugler LA. Matrix metalloproteinases in the equine systemic inflammatory response: implications for equine laminitis. [Doctoral Dissertation]. Louisiana State University; 2008. Available from: etd-01212009-182854 ; https://digitalcommons.lsu.edu/gradschool_dissertations/908

3. Rouanet Mehouas, Cécile. Conception de ligands à vocation thérapeutique : combinaison d'approches multidisciplinaires pour comprendre les interactions intermoléculaires : Design of therapeutic compounds : Combination of multidisciplinary approaches to get deeper insight into intermolecular interactions.

Degree: Docteur es, Biochimie et biologie structurale, 2015, Université Paris-Saclay (ComUE)

URL: http://www.theses.fr/2015SACLS145

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Les mécanismes de reconnaissance moléculaire sont à la base de nombreuses fonction biologiques essentielles (transduction du signal, régulation de l’expression génique, stimulation du système immunitaire,...).… (more)

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Les mécanismes de reconnaissance moléculaire sont à la base de nombreuses fonction biologiques essentielles (transduction du signal, régulation de l’expression génique, stimulation du système immunitaire,...). La compréhension des phénomènes physiques et chimiques à la base de ces phénomènes est fondamentale pour de nombreuses applications telles que la conception de médicaments, le développement d’outils diagnostiques ou tout autre procédé biotechnologique. Dans cette étude, nous avons étudié de manière extensive l’interaction entre la métalloélastase du macrophage (MMP-12) et le RXP470.1, un inhibiteur puissant et sélectif. En combinant des approches de cristallographie, de microcalorimétrie (ITC) et des tests enzymatiques, nous avons pu quantifier l’importance énergétique du transfert d’un seul proton suite à la liaison du RXP470.1, et mettre en lumière l’importance des contributions entropiques. Ainsi, la protonation du Glu219, un résidu catalytique, permet de compenser une enthalpie de liaison intrinsèque défavorable. Cette protonation est rendue possible par le large shift de pKa que subit le Glu219 en réponse à la liaison du RXP470.1 (pKalibre = 5.7 ± 0.1 / pKalié = 10 ± 0.04). Enfin, cette étude est la première, à notre connaissance, ayant combiné données d’affinité, thermodynamiques et structurales pour aborder le rôle du groupe chélatant. Nous avons ainsi étudié deux analogues du RXP470.1 variant seulement par la nature de leur pince à zinc. Ces modifications se traduisent par un effet marqué sur les profils d’affinité et de sélectivité ainsi que sur la signature énergétique des composés étudiés. L’étude des facteurs B, associés à l’analyse des structures cristallographiques, de ces inhibiteurs en complexe avec la MMP-12, suggère que des différences mineures de structures peuvent engendrer des variations de mobilité importantes au niveau des résidus impliqués dans l’interaction inhibiteur - enzyme. Ces différences trouvent leur origine dans un positionnement très légèrement différent du groupe chélatant par rapport au zinc.Pris dans leur ensemble, ces résultats pointent la nécessité de combiner un ensemble d’approches expérimentales pour décrire la complexité des interactions protéine/ligand. Ces associations doivent permettre d’évaluer le potentiel de méthodes théoriques capables de décrire des systèmes complexes.

Protein-ligand recognition mechanisms are essential to many fundamental biological functions such as signal transduction, gene regulation or stimulation of the immune system. Understanding the physical and chemical phenomenon upon protein-ligand binding is essential for many practical applications such as drug design, ligand based diagnostic tools and any other study based on biotechnology. In this study, we extensively explored the interaction between human macrophage metallo elastase MMP-12 and RXP470.1, a potent and selective inhibitor. By combining high resolution X-ray crystallography, FRET based enzyme assays and Isothermal Titration Calorimetry, we were able to highlight the importance…

Advisors/Committee Members: Dive, Vincent (thesis director).

Subjects/Keywords: Mmp-12; Inhibition; Protonation; Itc; Cristallographie; Groupement chélatant du Zinc; Mmp-12; Inhibition; Protonation; Itc; Crystallography; Zinc Binding Group

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Rouanet Mehouas, C. (2015). Conception de ligands à vocation thérapeutique : combinaison d'approches multidisciplinaires pour comprendre les interactions intermoléculaires : Design of therapeutic compounds : Combination of multidisciplinary approaches to get deeper insight into intermolecular interactions. (Doctoral Dissertation). Université Paris-Saclay (ComUE). Retrieved from http://www.theses.fr/2015SACLS145

Chicago Manual of Style (16^th Edition):

Rouanet Mehouas, Cécile. “Conception de ligands à vocation thérapeutique : combinaison d'approches multidisciplinaires pour comprendre les interactions intermoléculaires : Design of therapeutic compounds : Combination of multidisciplinary approaches to get deeper insight into intermolecular interactions.” 2015. Doctoral Dissertation, Université Paris-Saclay (ComUE). Accessed March 08, 2021. http://www.theses.fr/2015SACLS145.

MLA Handbook (7^th Edition):

Rouanet Mehouas, Cécile. “Conception de ligands à vocation thérapeutique : combinaison d'approches multidisciplinaires pour comprendre les interactions intermoléculaires : Design of therapeutic compounds : Combination of multidisciplinary approaches to get deeper insight into intermolecular interactions.” 2015. Web. 08 Mar 2021.

Vancouver:

Rouanet Mehouas C. Conception de ligands à vocation thérapeutique : combinaison d'approches multidisciplinaires pour comprendre les interactions intermoléculaires : Design of therapeutic compounds : Combination of multidisciplinary approaches to get deeper insight into intermolecular interactions. [Internet] [Doctoral dissertation]. Université Paris-Saclay (ComUE); 2015. [cited 2021 Mar 08]. Available from: http://www.theses.fr/2015SACLS145.

Council of Science Editors:

Rouanet Mehouas C. Conception de ligands à vocation thérapeutique : combinaison d'approches multidisciplinaires pour comprendre les interactions intermoléculaires : Design of therapeutic compounds : Combination of multidisciplinary approaches to get deeper insight into intermolecular interactions. [Doctoral Dissertation]. Université Paris-Saclay (ComUE); 2015. Available from: http://www.theses.fr/2015SACLS145

4. Talungchit, Supitcha. Enhancing resin-dentin bond effectiveness and durability: the role of ethanol-wet bonding technique, MMP-inhibition (chlorhexidine), and photoinitiator systems.

Degree: PhD, Oral Science, 2012, University of Iowa

URL: https://ir.uiowa.edu/etd/2996

► Current hydrophilic resin adhesives undergo hydrolytic degradation and show a decrease in bond strength over time. Nanoleakage and ultrastructure studies suggest that inadequately infiltrated… (more)

▼ Current hydrophilic resin adhesives undergo hydrolytic degradation and show a decrease in bond strength over time. Nanoleakage and ultrastructure studies suggest that inadequately infiltrated collagen leads to enzymatic degradation and resin-dentin bond failure. Adequate degree of conversion (DC) of resin adhesives is also critical to resin-dentin bond strength and durability. The long-term goal of this dissertation is the realization of durable resin-dentin bond. It is hypothesized that ethanol-wet bonding technique (EW) may effectively facilitate the infiltration of hydrophobic monomers into hydrophilic acid-etched dentin by maintaining interfibrillar spacing, stiffening collagen matrix, and improving adhesive resin-demineralized dentin matrix miscibility. Chlorhexidine (CHX), Matrix Metalloproteinase-inhibitor (MMP-inhibitor), should further preserve collagen integrity and resin-dentin bond strength. Moreover, efficient photoinitiator systems that broaden light absorptivity and provide more reactive radicals may enhance polymerization. In this dissertation, a clinically-relevant EW protocol, 3×15s absolute ethanol rinsing, provided significantly higher microtensile bond strength (πTBS) of a hydrophobic resin (70%BisGMA/30%TEGDMA) to dentin as compared to water-wet bonding (WW). All groups showed no significant drop of πTBS after 1-year storage except EW without CHX application, showing marginally significant reduction in πTBS (p=0.0558) suggesting MMP-inhibition by CHX in EW. These results were consistent with subsequent experiments. EW maintained interfibrillar width and hybrid layer thickness for resin infiltration and retention. Monomer molar concentration across the hybrid layer was significantly higher in EW than WW. An application of 2% CHX diacetate further preserved collagen banding in EW. WW showed more generalized spotted nanoleakage, while EW presented localized reticular nanoleakage. The use of Irgacure 819 (BAPO) alone and in combination with benzoyl peroxide (BPO) or camphorquinone (CQ) increased DC of hydrophobic and hydrophilic resins over resins containing the CQ/amine (4E) control. Only BAPO and BAPO/BPO demonstrated significantly higher immediate shear bond strength than CQ/4E. Within the limitations of these studies, EW improved resin-dentin bond durability by maintaining collagen interfibrillar spaces for efficient infiltration of a hydrophobic BisGMA/TEGDMA resin resulting in significantly higher πTBS and monomer molar concentrations with less nanoleakage distribution within the hybrid layer than WW. CHX further maintained collagen integrity and πTBS in EW. BAPO is a potential… Advisors/Committee Members: Armstrong, Steven R. (supervisor).

Subjects/Keywords: Chlorhexidine; Dentin adhesion; Ethanol-wet bonding; MMP-inhibition; Photoinitiator; Resin adhesives; Oral Biology and Oral Pathology

…significant reduction in μTBS (p=0.0558) suggesting MMP-inhibition by CHX in EW. These… …inhibitor (MMP-inhibitor), should further preserve collagen integrity and resin-dentin… …105 Table 2-4 Weibull parameters by the type of combination of bonding substrate and MMP… …inhibition at same day… …108 Table 2-5 Weibull parameters by the type of combination of bonding substrate and MMP…

10 more images…

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Talungchit, S. (2012). Enhancing resin-dentin bond effectiveness and durability: the role of ethanol-wet bonding technique, MMP-inhibition (chlorhexidine), and photoinitiator systems. (Doctoral Dissertation). University of Iowa. Retrieved from https://ir.uiowa.edu/etd/2996

Chicago Manual of Style (16^th Edition):

Talungchit, Supitcha. “Enhancing resin-dentin bond effectiveness and durability: the role of ethanol-wet bonding technique, MMP-inhibition (chlorhexidine), and photoinitiator systems.” 2012. Doctoral Dissertation, University of Iowa. Accessed March 08, 2021. https://ir.uiowa.edu/etd/2996.

MLA Handbook (7^th Edition):

Talungchit, Supitcha. “Enhancing resin-dentin bond effectiveness and durability: the role of ethanol-wet bonding technique, MMP-inhibition (chlorhexidine), and photoinitiator systems.” 2012. Web. 08 Mar 2021.

Vancouver:

Talungchit S. Enhancing resin-dentin bond effectiveness and durability: the role of ethanol-wet bonding technique, MMP-inhibition (chlorhexidine), and photoinitiator systems. [Internet] [Doctoral dissertation]. University of Iowa; 2012. [cited 2021 Mar 08]. Available from: https://ir.uiowa.edu/etd/2996.

Council of Science Editors:

Talungchit S. Enhancing resin-dentin bond effectiveness and durability: the role of ethanol-wet bonding technique, MMP-inhibition (chlorhexidine), and photoinitiator systems. [Doctoral Dissertation]. University of Iowa; 2012. Available from: https://ir.uiowa.edu/etd/2996

University of Lund

5. Rehn, Martin. Experimental Colonic Obstruction and Anastomotic Healing.

Degree: 2012, University of Lund

URL: https://lup.lub.lu.se/record/2299871 ; https://portal.research.lu.se/ws/files/3661064/2299872.pdf

► Colorectal cancer is one of the most common malignant diseases world-wide. Most patients can be diagnosed, staged and treated by surgery in an elective setting.… (more)

Subjects/Keywords: Surgery; Obstruction; anastomosis; colon; wound healing; matrix metalloproteinases; collagen; MMP inhibition

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Rehn, M. (2012). Experimental Colonic Obstruction and Anastomotic Healing. (Doctoral Dissertation). University of Lund. Retrieved from https://lup.lub.lu.se/record/2299871 ; https://portal.research.lu.se/ws/files/3661064/2299872.pdf

Chicago Manual of Style (16^th Edition):

Rehn, Martin. “Experimental Colonic Obstruction and Anastomotic Healing.” 2012. Doctoral Dissertation, University of Lund. Accessed March 08, 2021. https://lup.lub.lu.se/record/2299871 ; https://portal.research.lu.se/ws/files/3661064/2299872.pdf.

MLA Handbook (7^th Edition):

Rehn, Martin. “Experimental Colonic Obstruction and Anastomotic Healing.” 2012. Web. 08 Mar 2021.

Vancouver:

Rehn M. Experimental Colonic Obstruction and Anastomotic Healing. [Internet] [Doctoral dissertation]. University of Lund; 2012. [cited 2021 Mar 08]. Available from: https://lup.lub.lu.se/record/2299871 ; https://portal.research.lu.se/ws/files/3661064/2299872.pdf.

Council of Science Editors:

Rehn M. Experimental Colonic Obstruction and Anastomotic Healing. [Doctoral Dissertation]. University of Lund; 2012. Available from: https://lup.lub.lu.se/record/2299871 ; https://portal.research.lu.se/ws/files/3661064/2299872.pdf

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