subject:(MMP 9)

NSYSU

1. Liu, Yi-Jia. Study of Kazal motifs of RECK protein on MMP-2 and MMP-9 activity and metastasis of lung adenocarcinoma.

Degree: Master, Institute of Biomedical Sciences, 2009, NSYSU

URL: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0806109-130653

► RECK stands for âreversion-inducing cysteine-rich protein with Kazal motifsâ. This gene was initially discovered by screening a human fibroblast cDNA library for genes giving rise… (more)

Subjects/Keywords: RECK; MMP-9

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Liu, Y. (2009). Study of Kazal motifs of RECK protein on MMP-2 and MMP-9 activity and metastasis of lung adenocarcinoma. (Thesis). NSYSU. Retrieved from http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0806109-130653

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Liu, Yi-Jia. “Study of Kazal motifs of RECK protein on MMP-2 and MMP-9 activity and metastasis of lung adenocarcinoma.” 2009. Thesis, NSYSU. Accessed March 09, 2021. http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0806109-130653.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Liu, Yi-Jia. “Study of Kazal motifs of RECK protein on MMP-2 and MMP-9 activity and metastasis of lung adenocarcinoma.” 2009. Web. 09 Mar 2021.

Vancouver:

Liu Y. Study of Kazal motifs of RECK protein on MMP-2 and MMP-9 activity and metastasis of lung adenocarcinoma. [Internet] [Thesis]. NSYSU; 2009. [cited 2021 Mar 09]. Available from: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0806109-130653.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Liu Y. Study of Kazal motifs of RECK protein on MMP-2 and MMP-9 activity and metastasis of lung adenocarcinoma. [Thesis]. NSYSU; 2009. Available from: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0806109-130653

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

2. Reis, Sabrina Thalita dos. Análise da expressão de MMP-2, MMP-9, MT1-MMP (MMP-14), TIMP-1, TIMP-2, RECK, TGF-Beta e interleucina-8 em câncer de próstata.

Degree: PhD, Urologia, 2011, University of São Paulo

URL: http://www.teses.usp.br/teses/disponiveis/5/5153/tde-22092011-133255/ ;

► Introdução: O câncer de próstata (CaP) é o tumor mais freqüente do homem no Brasil tendo sido estimados mais de 52.350 novos casos em 2010,… (more)

▼ Introdução: O câncer de próstata (CaP) é o tumor mais freqüente do homem no Brasil tendo sido estimados mais de 52.350 novos casos em 2010, sendo a segunda causa de óbito por câncer em homens. O prognóstico depende fundamentalmente dos níveis séricos de Prostatic Specific Antigen (PSA) estádio tumoral (TNM) e grau de diferenciação histológica (Gleason). Porém esses têm sido insuficientes na definição do prognóstico da neoplasia. Por isso pesquisas têm sido direcionadas para a identificação de alterações moleculares que possam prever o potencial de agressividade do câncer de próstata. Metaloproteinases da matriz (MMP) são proteínas pertencentes a uma família de aproximadamente 30 enzimas proteolíticas ou endoproteinases que degradam vários componentes da matriz extracelular. A detecção de sua expressão tem sido estudada como marcador sensível e específico de vários tumores, principalmente as MMP pertencentes ao grupo das gelatinases MMP-2 e MMP-9. Objetivo: o objetivo deste nosso trabalho foi avaliarmos pela técnica de qRT-PCR e imuno-histoquímica os níveis de expressão dos genes das MMP pertencentes ao grupo das gelatinases, MMP-2 e MMP-9, bem como outros sabidamente envolvidos em suas vias de ativação (MMP-14, IL-8) e inibição (TIMP-1, TIMP-2, RECK e TGF-) no câncer localizado de próstata. Material e Métodos: O estudo consistiu na análise de espécimes de 79 pacientes com câncer da próstata submetidos a prostatectomia radical entre setembro de 1997 e fevereiro de 2000. Esses oito genes foram então testados quanto a seu valor prognóstico no câncer da próstata através da técnica de reação em cadeia da polimerase quantitativa com transcriptase reversa (qRT-PCR). Análise proteica foi feita a partir de 40 pacientes deste pool. O grupo controle foi composto de tecido de 11 pacientes com hiperplasia benigna da próstata (HPB) tratados cirurgicamente com prostatectomia retropúbica. Resultados: MMP-9 esteve superexpressa e MMP-2, TIMP-1, TIMP-2, MMP 14, IL-8, TGF- e RECK se mostraram subexpressos em tecido representativo de CaP quando comparado com HPB. A análise dos níveis de expressão dos genes com o escore de Gleason, mostrou que MMP-2 e TIMP-2 mesmo mantendo-se subexpressos, tiveram uma expressão maior entre os pacientes que apresentavam Gleason 7 (p=0,04 e p=0,02 respectivamente). De acordo com o valor de PSA préoperatório, encontramos diferenças na expressão de MMP-9. Pacientes que apresentavam um PSA pré-operatório 10 ng/mL possuíam uma mediana de expressão maior que aqueles cujo PSA pré-operatório <10 ng/mL com medianas de expressão de 5,62 e 2,76 respectivamente (p=0,033). Não encontramos diferenças estatísticas entre pacientes que apresentavam ou não recidiva bioquímica quanto a expressão dos 8 genes estudados. Porém o gene da MMP-9 apresentou uma diferença estatística marginal apresentando uma mediana de expressão de 6,29x nos pacientes que apresentaram recidiva bioquímica e de 3,25 nos pacientes que não apresentaram recidiva bioquímica (p=0,090). De acordo com a expressão proteica, encontramos uma maior… Advisors/Committee Members: Antunes, Alberto Azoubel, Leite, Katia Ramos Moreira.

Subjects/Keywords: Expressão gênica; Gene expression; Metaloproteinases; MMP- 2; MMP-2; MMP-9; MMP-9; Neoplasias prostáticas; Prognosis; Prognóstico; Próstata; Prostate; Prostate neoplasms

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Reis, S. T. d. (2011). Análise da expressão de MMP-2, MMP-9, MT1-MMP (MMP-14), TIMP-1, TIMP-2, RECK, TGF-Beta e interleucina-8 em câncer de próstata. (Doctoral Dissertation). University of São Paulo. Retrieved from http://www.teses.usp.br/teses/disponiveis/5/5153/tde-22092011-133255/ ;

Chicago Manual of Style (16^th Edition):

Reis, Sabrina Thalita dos. “Análise da expressão de MMP-2, MMP-9, MT1-MMP (MMP-14), TIMP-1, TIMP-2, RECK, TGF-Beta e interleucina-8 em câncer de próstata.” 2011. Doctoral Dissertation, University of São Paulo. Accessed March 09, 2021. http://www.teses.usp.br/teses/disponiveis/5/5153/tde-22092011-133255/ ;.

MLA Handbook (7^th Edition):

Reis, Sabrina Thalita dos. “Análise da expressão de MMP-2, MMP-9, MT1-MMP (MMP-14), TIMP-1, TIMP-2, RECK, TGF-Beta e interleucina-8 em câncer de próstata.” 2011. Web. 09 Mar 2021.

Vancouver:

Reis STd. Análise da expressão de MMP-2, MMP-9, MT1-MMP (MMP-14), TIMP-1, TIMP-2, RECK, TGF-Beta e interleucina-8 em câncer de próstata. [Internet] [Doctoral dissertation]. University of São Paulo; 2011. [cited 2021 Mar 09]. Available from: http://www.teses.usp.br/teses/disponiveis/5/5153/tde-22092011-133255/ ;.

Council of Science Editors:

Reis STd. Análise da expressão de MMP-2, MMP-9, MT1-MMP (MMP-14), TIMP-1, TIMP-2, RECK, TGF-Beta e interleucina-8 em câncer de próstata. [Doctoral Dissertation]. University of São Paulo; 2011. Available from: http://www.teses.usp.br/teses/disponiveis/5/5153/tde-22092011-133255/ ;

3. Vanessa Christina Santos Pavesi. Efeito da criolesão no remodelamento da matriz extracelular em músculo esquelético de rato.

Degree: 2008, Universidade Nove de Julho

URL: http://www4.uninove.br/tedeSimplificado/tde_busca/arquivo.php?codArquivo=249

►

The aim of the present study was to investigate components of extracellular matrix (ECM) during the skeletal muscle regeneration after cryolesion in rats. Sixty adult… (more)

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The aim of the present study was to investigate components of extracellular matrix (ECM) during the skeletal muscle regeneration after cryolesion in rats. Sixty adult Wistar rats were used. The animals were divided into 4 groups: 1, 7, 14 and 21 days post-injury (10animls/group). One of the tibialis anterior muscles was cryolesioned and the contralateral muscle was used as the control. One sham group, in which animals received only the surgical procedure and one group of control animals that do not receive any intervention were included. The muscles were submitted to hematoxylin-eosin and immunohistochemistry staining for collagen IV, MMP-2 and MMP-9 detection. The MMP enzymatic activity was verified by zymography. The control muscles showed normal morphology and the sham group exhibited an inflammatory infiltrated. After 1 day, the cryolesioned group exhibit moderate inflammatory infiltrated and necrosis areas. At 7 and 14 days a significant reduction of the inflammatory process, absence of necrosis and presence of immature muscular cells could be observed. The injured group revealed total remodeling after the 21 days. Collagen IV showed a disordered distribution pattern after 1 day of injury and gradually it was reorganized during the 21 days of the muscle regeneration. The MMP-2 and MMP-9 immunolabeling, as well, the MMP-2 activity were related to the phase of skeletal muscle repair. After 1 day, an increase on MMP-2 and MMP-9 labeling and MMP-2 activity were observed. In conclusion, the increase and decrease of immunolabeling and activity of MMP were related to the break down and accumulation of collagen IV during the skeletal muscle remodeling.

O objetivo deste estudo foi investigar os componentes da matriz extracelular (MEC) durante a regeneração muscular esquelética. Foram utilizados 60 ratos Wistar sendo que destes, foram organizados 4 grupos avaliados 1, 7, 14 e 21 dias após a lesão (10 animais/grupo). Esses grupos foram submetidos à criolesão no músculo tibial anterior do lado esquerdo e os músculos contralaterais foram os controles. Foi realizado um grupo sham, que recebeu apenas o procedimento cirúrgico e um grupo de animais controle que não recebeu nenhuma intervenção. Os músculos foram submetidos à colorações por hematoxilina &eosina e imuno-histoquímica para detecção de colágeno tipo IV, MMP-2 e MMP-9. A atividade enzimática de MMPs foi verificada pela zimografia. Os músculos controle mostraram morfologia normal, o grupo sham exibiu leve infiltrado inflamatório. No grupo criolesionado foi observado após 1 dia moderado infiltrado inflamatório e áreas de necrose. Após 7 e 14 dias pode-se observar redução significativa do processo inflamatório, ausência de necrose e presença de células musculares imaturas. Aos 21 dias o músculo mostrou evidência de reparo completo. O colágeno IV mostrou-se desorganizado por entre as fibras lesadas após 1 dia da criolesão e se reorganizou gradualmente ao longo dos 21 dias. A imunoreatividade da MMP-2 e MMP-9, assim como a atividade de MMP-2 se mostraram relacionadas com…

Advisors/Committee Members: Manoela Domingues Martins, Raquel Agnelli Mesquita Ferrari, Carlos Gilberto Silva, Helen Miyabara.

Subjects/Keywords: MMP-2, MMP-9; Colágeno IV; OUTROS; Matriz Extracelular; Remodelamento Muscular; Collagen IV; Extracellular Matrix; Muscle Remodeling; MMP-2, MMP-9

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Pavesi, V. C. S. (2008). Efeito da criolesão no remodelamento da matriz extracelular em músculo esquelético de rato. (Thesis). Universidade Nove de Julho. Retrieved from http://www4.uninove.br/tedeSimplificado/tde_busca/arquivo.php?codArquivo=249

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Pavesi, Vanessa Christina Santos. “Efeito da criolesão no remodelamento da matriz extracelular em músculo esquelético de rato.” 2008. Thesis, Universidade Nove de Julho. Accessed March 09, 2021. http://www4.uninove.br/tedeSimplificado/tde_busca/arquivo.php?codArquivo=249.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Pavesi, Vanessa Christina Santos. “Efeito da criolesão no remodelamento da matriz extracelular em músculo esquelético de rato.” 2008. Web. 09 Mar 2021.

Vancouver:

Pavesi VCS. Efeito da criolesão no remodelamento da matriz extracelular em músculo esquelético de rato. [Internet] [Thesis]. Universidade Nove de Julho; 2008. [cited 2021 Mar 09]. Available from: http://www4.uninove.br/tedeSimplificado/tde_busca/arquivo.php?codArquivo=249.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Pavesi VCS. Efeito da criolesão no remodelamento da matriz extracelular em músculo esquelético de rato. [Thesis]. Universidade Nove de Julho; 2008. Available from: http://www4.uninove.br/tedeSimplificado/tde_busca/arquivo.php?codArquivo=249

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Helsinki

4. Vakkamäki, Johanna. Oriin seminaaliplasman matriksimetalloproteinaasit.

Degree: Department of Clinical Production Animal Medicine; Helsingin yliopisto, Eläinlääketieteellinen tiedekunta, Kliinisen tuotantoeläinlääketieteen osasto; Helsingfors universitet, Veterinärmedicinska fakulteten, Avdelningen för klinisk produktionsdjursmedicin, 2011, University of Helsinki

URL: http://hdl.handle.net/10138/26742

► Siirto- ja pakastesiemennysten lisääntyessä tammojen hedelmällisyystulosten parantuminen on hevoskasvatuksen taloudellisuuden kannalta merkittävää. Oriitten spermoissa on eroja sekä siirto- että pakastekestävyydessä. Pelkällä orivalinnalla ei voida kuitenkaan… (more)

▼ Siirto- ja pakastesiemennysten lisääntyessä tammojen hedelmällisyystulosten parantuminen on hevoskasvatuksen taloudellisuuden kannalta merkittävää. Oriitten spermoissa on eroja sekä siirto- että pakastekestävyydessä. Pelkällä orivalinnalla ei voida kuitenkaan vaikuttaa hedelmällisyystuloksiin, sillä yleensä valinta painottuu suorituskykyyn. Siittiöiden lisäksi seminaaliplasmalla on havaittu vaikutuksia siirto- ja pakastuskestävyyteen. Seminaaliplasma koostuu useista erilaisista biologisista komponenteista, joista matriksimetalloproteinaasit (MMP) ovat yksi. Ne ovat proteiiniperhe, johon kuluu useita jäseniä. MMP:t kykenevät hajottamaan muun muassa solun ulkoisia tukirakenteita sekä tyvikalvoa erilaisissa fysiologisissa ja patologisissa tiloissa. Matriksimetalloproteinaaseja on löydetty useista kudoksista ja myös seminaaliplasmasta. Työn tutkimusosuudessa haluttiin selvittää MMP-pitoisuuksia, niiden vaihteluita oriitten välillä ja mahdollisia vaikutuksia hedelmällisyystuloksiin. Seminaaliplasmanäytteitä tutkittiin yhteensä 43 oriista. Näytteet oli kerätty astutuskaudella 2006 erirotuisilta ja -ikäisiltä hevosilta sekä kahdelta ponilta. Keräysvaiheessa näytteet jaoteltiin 1-4 eri fraktioon. Jokaisesta näytteestä tutkittiin MMP:t zymografian avulla. Seminaaliplasmanäytteiden lisäksi oriilta kerättiin hedelmällisyystietoja siittoloista sekä Suomen raviurheilun ja hevoskasvatuksen keskusjärjestöltä (Suomen Hippos ry). Kaikki tulokset taulukoitiin ja laskettiin aktiiviselle (akt-MMP-2) ja pro-MMP-2:lle sekä kokonais-MMP-9:lle (tot-MMP-9) siittiörikkaassa (SR) ja siittiököyhässä fraktiossa (SK) sekä kokonaisejakulaatissa (KE): keskiarvot, keskihajonnat, mediaanit sekä maksimi- ja minimiarvot. Spearmanin järjestyskorrelaatiokertoimet laskettiin tammojen ensimmäiseen kiimaan tiinehtymisen ja eri MMP-pitoisuuksien välille SR:ssa ja KE:ssa. Oriilla oli havaittavia pitoisuuksia pro- ja akt-MMP-2:ta sekä tot-MMP-9:ää. MMP-pitoisuudet olivat suurimmat SR:ssa. Suurimpia olivat pro-MMP-2:n pitoisuudet ja orikohtaiset erot olivat siinä pieniä. Saadut tulokset vastasivat odotuksia, sillä miesten seminaaliplasmatutkimusten tulokset ovat samansuuntaisia. Eri MMP-pitoisuuksilla ei havaittu korrelaatiota tammojen tiinehtymiseen kanssa. Aineiston pienen koon takia sattumalla voi olla suuri vaikutus tuloksiin. Myös keräysvuodenaika ja yksilön ejakulaation koostumusvaihtelut saattavat vaikuttaa seminaaliplasman MMP-pitoisuuksiin, sillä sen useissa ominaisuuksissa tapahtuu muutoksia näiden muuttujien mukaan. Tulokset ovat suuntaa antavia ja toimivat apuna jatkotutkimuksia suunniteltaessa.

Subjects/Keywords: seminaaliplasma; MMP-2; MMP-9; oriit; hevonen; Kotieläinten lisääntymistiede; Husdjurens reproduktion; Reproduction of Domestic Animals; seminaaliplasma; MMP-2; MMP-9

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Vakkamäki, J. (2011). Oriin seminaaliplasman matriksimetalloproteinaasit. (Thesis). University of Helsinki. Retrieved from http://hdl.handle.net/10138/26742

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Vakkamäki, Johanna. “Oriin seminaaliplasman matriksimetalloproteinaasit.” 2011. Thesis, University of Helsinki. Accessed March 09, 2021. http://hdl.handle.net/10138/26742.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Vakkamäki, Johanna. “Oriin seminaaliplasman matriksimetalloproteinaasit.” 2011. Web. 09 Mar 2021.

Vancouver:

Vakkamäki J. Oriin seminaaliplasman matriksimetalloproteinaasit. [Internet] [Thesis]. University of Helsinki; 2011. [cited 2021 Mar 09]. Available from: http://hdl.handle.net/10138/26742.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Vakkamäki J. Oriin seminaaliplasman matriksimetalloproteinaasit. [Thesis]. University of Helsinki; 2011. Available from: http://hdl.handle.net/10138/26742

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Queens University

5. Alghamdi, Farah. Neuraminidase-1 Sialidase and Matrix Metalloproteinase-9 Crosstalk in Alliance With Insulin Receptors is an Essential Molecular Signaling Platform for Insulin-Induced Receptor Activation .

Degree: Microbiology and Immunology, 2013, Queens University

URL: http://hdl.handle.net/1974/7820

► Molecular-targeting therapeutics directed towards growth factor receptors have become promising interventions in cancer. They include the family of mammalian receptor tyrosine kinases such as epidermal… (more)

Subjects/Keywords: Insulin Receptors ; MMP-9 ; NEURAMINIDASE-1

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Alghamdi, F. (2013). Neuraminidase-1 Sialidase and Matrix Metalloproteinase-9 Crosstalk in Alliance With Insulin Receptors is an Essential Molecular Signaling Platform for Insulin-Induced Receptor Activation . (Thesis). Queens University. Retrieved from http://hdl.handle.net/1974/7820

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Alghamdi, Farah. “Neuraminidase-1 Sialidase and Matrix Metalloproteinase-9 Crosstalk in Alliance With Insulin Receptors is an Essential Molecular Signaling Platform for Insulin-Induced Receptor Activation .” 2013. Thesis, Queens University. Accessed March 09, 2021. http://hdl.handle.net/1974/7820.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Alghamdi, Farah. “Neuraminidase-1 Sialidase and Matrix Metalloproteinase-9 Crosstalk in Alliance With Insulin Receptors is an Essential Molecular Signaling Platform for Insulin-Induced Receptor Activation .” 2013. Web. 09 Mar 2021.

Vancouver:

Alghamdi F. Neuraminidase-1 Sialidase and Matrix Metalloproteinase-9 Crosstalk in Alliance With Insulin Receptors is an Essential Molecular Signaling Platform for Insulin-Induced Receptor Activation . [Internet] [Thesis]. Queens University; 2013. [cited 2021 Mar 09]. Available from: http://hdl.handle.net/1974/7820.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Alghamdi F. Neuraminidase-1 Sialidase and Matrix Metalloproteinase-9 Crosstalk in Alliance With Insulin Receptors is an Essential Molecular Signaling Platform for Insulin-Induced Receptor Activation . [Thesis]. Queens University; 2013. Available from: http://hdl.handle.net/1974/7820

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

6. Hallett, Miranda A. The Treatment of Breast Cancer Tumor Growth and Metastasis With an Anti-MMP9 DNAzyme.

Degree: PhD, Biomedical Sciences, 2011, University of Tennessee Health Science Center

URL: https://dc.uthsc.edu/dissertations/110

► BACKGROUND: Tumor cell proliferation, invasion and metastasis are known to be mediated, at least in part, through degradation of basement membrane by neutral metalloproteinases… (more)

Subjects/Keywords: Breast Cancer; DNAzyme; Metastasis; MMP-9

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Hallett, M. A. (2011). The Treatment of Breast Cancer Tumor Growth and Metastasis With an Anti-MMP9 DNAzyme. (Doctoral Dissertation). University of Tennessee Health Science Center. Retrieved from https://dc.uthsc.edu/dissertations/110

Chicago Manual of Style (16^th Edition):

Hallett, Miranda A. “The Treatment of Breast Cancer Tumor Growth and Metastasis With an Anti-MMP9 DNAzyme.” 2011. Doctoral Dissertation, University of Tennessee Health Science Center. Accessed March 09, 2021. https://dc.uthsc.edu/dissertations/110.

MLA Handbook (7^th Edition):

Hallett, Miranda A. “The Treatment of Breast Cancer Tumor Growth and Metastasis With an Anti-MMP9 DNAzyme.” 2011. Web. 09 Mar 2021.

Vancouver:

Hallett MA. The Treatment of Breast Cancer Tumor Growth and Metastasis With an Anti-MMP9 DNAzyme. [Internet] [Doctoral dissertation]. University of Tennessee Health Science Center; 2011. [cited 2021 Mar 09]. Available from: https://dc.uthsc.edu/dissertations/110.

Council of Science Editors:

Hallett MA. The Treatment of Breast Cancer Tumor Growth and Metastasis With an Anti-MMP9 DNAzyme. [Doctoral Dissertation]. University of Tennessee Health Science Center; 2011. Available from: https://dc.uthsc.edu/dissertations/110

NSYSU

7. Chen, Ying-Jung. Molecular regulation of metalloproteinases (MMP-2, MMP-9 and ADAM17) expression in human leukemia cells.

Degree: PhD, Institute of Biomedical Sciences, 2014, NSYSU

URL: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0324114-153506

► Metalloproteinases regulate aspects of cell development, effector function, migration, and ligand-receptor interactions. Membrane-anchored disintegrin metalloproteinases (ADAMs) are transmembrane proteins which carry out ectodomain shedding of… (more)

▼ Metalloproteinases regulate aspects of cell development, effector function, migration, and ligand-receptor interactions. Membrane-anchored disintegrin metalloproteinases (ADAMs) are transmembrane proteins which carry out ectodomain shedding of cytokines and their congnate receptors. Matrix metalloproteinases (MMPs) became known as the primart class of enzymes responsible for degradation of extracellular matrix protein. The main role of MMPs in angiogenesis, tumor growth, and metastasis is degradation of extracellular matrix and release and/or activation of growth factors through their degradative activity. Mounting evidence supports the view that extracellular proteinases mediate many of the change in the microenviroment during tumor progression. These enzymes regulate a variety of physiological processes and signaling events, and thus they represent key players in the molecular communication between tumor and stroma. In this thesis, arecoline, hydroquinone, gallic acid, and simvastatin are employed to investigate the molecular mechanisms in regulating ADAM17 and MMP-2/MMP-9 of leukemic cell line K562. Arecoline is an alkaloid extracted form betel nuts. Arecoline have been shown to be characterized by carcinogenicity, cytotoxicity, immunotoxicity, and genotoxicity. Our data revealed that arecoline up-regulated transcriptional level of TNFR2 mRNA via JNK/c-Jun pathway activation in K562 cells. Moreover, arecoline-induced down regulation of mature ADAM17 reduced TNFR2 shedding. Up-modulation of TNFR2 surface expression is associated with arecoline-induced death of K562 cells. Hydroquinone, a major marrow metabolite of leukemogen benzene, leading to myelotoxicity and benzene-related hematogical disorders. Hydroquinone induced down-regulation of miR-122 expression, leading to ADAM17 up-regultion and ADAM17-mediated TNF-Î± shedding. Gallic acid as a polyhdyroyxlphenolic compound is widely distributed in various plants, fruits, and foods. Various biological activities of gallic acid have been reported, including antibacterial, anti-inflammatory, and anticancer activities. MMP-2 and MMP-9 downregulation in gallic acid treated K562 cells were mediated throuht suppression of JNK1-mediated c-Jun/ATF-2 and Akt/ERK-mediated c-Jun/c-Fos pathways, respectively. Simvastatin is commonly prescribed cholesterol-lowering drug that significantly improve the morbidity and mortality associated with atherosclerosis. Privious studies show that simvastatin selectively inhibit MMP-9 expression but not MMP-2 expression in cancer cells. However, the mechanism remains elusive. Our date indicated that simvastatin-induced NFÎºB degradation led to MMP-9 down-regulation, while simvastatin-induced JNK1/c-Jun/ATF-2 activation kept MMP-2 expression underlying NFÎºB down-regulation. This aim of the study is to interpret the role of ADAM17, MMP-2 and MMP-9 in leukemia cells, focusing especially on their poteintial use as cancer biomarkers and therapeutic target. Advisors/Committee Members: Kuang-Hung Cheng (chair), Long-Sen Chang (committee member), Shinne-Ren Lin (chair), Chun-Chang Chang (chair), Shou-Mei Wu (chair).

Subjects/Keywords: Simvastatin; Gallic acid; Hydroquinone; MMP-9; MMP-2; ADAM17; Arecoline

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APA (6^th Edition):

Chen, Y. (2014). Molecular regulation of metalloproteinases (MMP-2, MMP-9 and ADAM17) expression in human leukemia cells. (Doctoral Dissertation). NSYSU. Retrieved from http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0324114-153506

Chicago Manual of Style (16^th Edition):

Chen, Ying-Jung. “Molecular regulation of metalloproteinases (MMP-2, MMP-9 and ADAM17) expression in human leukemia cells.” 2014. Doctoral Dissertation, NSYSU. Accessed March 09, 2021. http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0324114-153506.

MLA Handbook (7^th Edition):

Chen, Ying-Jung. “Molecular regulation of metalloproteinases (MMP-2, MMP-9 and ADAM17) expression in human leukemia cells.” 2014. Web. 09 Mar 2021.

Vancouver:

Chen Y. Molecular regulation of metalloproteinases (MMP-2, MMP-9 and ADAM17) expression in human leukemia cells. [Internet] [Doctoral dissertation]. NSYSU; 2014. [cited 2021 Mar 09]. Available from: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0324114-153506.

Council of Science Editors:

Chen Y. Molecular regulation of metalloproteinases (MMP-2, MMP-9 and ADAM17) expression in human leukemia cells. [Doctoral Dissertation]. NSYSU; 2014. Available from: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0324114-153506

University of Auckland

8. Ranasinghe, Himani Sumudumalee. Mechanisms underlying hypoxic ischemic injury to the developing brain: The significance of matrix metalloproteinase 2 and 9.

Degree: 2009, University of Auckland

URL: http://hdl.handle.net/2292/4962

► Perinatal hypoxic ischemic (HI) injury is a leading cause of long-term neurological complications in newborn babies. Matrix metalloproteinases (MMPs) are a family of endopeptidases that… (more)

Subjects/Keywords: Brain; Ischemia; Hypoxia; Injury; Developmental; MMP-9; MMP-2

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APA (6^th Edition):

Ranasinghe, H. S. (2009). Mechanisms underlying hypoxic ischemic injury to the developing brain: The significance of matrix metalloproteinase 2 and 9. (Doctoral Dissertation). University of Auckland. Retrieved from http://hdl.handle.net/2292/4962

Chicago Manual of Style (16^th Edition):

Ranasinghe, Himani Sumudumalee. “Mechanisms underlying hypoxic ischemic injury to the developing brain: The significance of matrix metalloproteinase 2 and 9.” 2009. Doctoral Dissertation, University of Auckland. Accessed March 09, 2021. http://hdl.handle.net/2292/4962.

MLA Handbook (7^th Edition):

Ranasinghe, Himani Sumudumalee. “Mechanisms underlying hypoxic ischemic injury to the developing brain: The significance of matrix metalloproteinase 2 and 9.” 2009. Web. 09 Mar 2021.

Vancouver:

Ranasinghe HS. Mechanisms underlying hypoxic ischemic injury to the developing brain: The significance of matrix metalloproteinase 2 and 9. [Internet] [Doctoral dissertation]. University of Auckland; 2009. [cited 2021 Mar 09]. Available from: http://hdl.handle.net/2292/4962.

Council of Science Editors:

Ranasinghe HS. Mechanisms underlying hypoxic ischemic injury to the developing brain: The significance of matrix metalloproteinase 2 and 9. [Doctoral Dissertation]. University of Auckland; 2009. Available from: http://hdl.handle.net/2292/4962

9. Michopoulou, Anna. Receptor syndecan-1 controls MMP-9 expression during keratinocyte migration : Le récepteur syndecan-1 contrôle l'expression de MMP-9 au cours de la migration des kératinocytes.

Degree: Docteur es, Biologie, 2016, Lyon

URL: http://www.theses.fr/2016LYSE1130

► La phase de l'épithélialisation de la réparation cutanée se déroule en impliquant plusieurs processus dynamiques et interactifs pendant lesquels les kératinocytes migrent, prolifèrent et se… (more)

▼ La phase de l'épithélialisation de la réparation cutanée se déroule en impliquant plusieurs processus dynamiques et interactifs pendant lesquels les kératinocytes migrent, prolifèrent et se différentient afin de reconstruire la fonction de la barrière. La migration des kératinocytes est l'événement qui détermine l'efficacité du processus entier. Le comportement migratoire est contrôlé au même temps au niveau extracellulaire et intracellulaire et dépend d'interactions dynamiques entre les cellules et leur environnement extracellulaire, des facteurs de croissance et des cytokines. Parmi les protéines de la matrice extracellulaire, la laminine 332 est un substrat d'adhésion majeur des kératinocytes qui joue un rôle important au cours de la migration des kératinocytes, travers son domaine LG4/5 localisé à l'extrémité carboxy-terminale de sa chaine a. Des études récentes ont rapporté que l'induction de la migration des kératinocytes par LG4/5 est dépendante des Métalloprotéinases Matricielles pro-migratoires (MMP)-9 et -1 qui jouent des rôles essentiels au cours de la cicatrisation et surtout pendant la ré-épithélialisation. Etant donné que des travaux antérieurs du laboratoire ont montré que le domaine LG4/5 participe à la dynamique du cytosquelette et à la motilité cellulaire au travers de liaisons avec les récepteurs de type de protéoglycanes à heparane sulfate, syndécan-1 et -4 on a regardé l'implication potentielle de ces récepteurs au processus. Afin d'analyser la participation possible des syndecans dans ce processus, nous avons développé une approche de mutagénèse dirigée dans la protéine LG4/5 recombinante pour altérer les sites de liaison aux syndécan-1 ou -4. Notre analyse PCR et nos résultats de zymographie ont révélé une différence du profile d'activation des MMPs en fonction de la mutation produite et donc de la capacité de la protéine à recruter le syndécan-1 ou le syndécan-4, ainsi que le syndécan-1, et pas la syndécan-4, est impliqué dans l'activation de la production de la MMP-9 par LG4/5. Nous avons ensuite confirmé ces résultats en réduisant l'expression du syndécan-1 dans des kératinocytes et on a pu aussi montrer que le traitement avec des cytokines telles que TNFalpha et IL-1beta, connues pour leur capacité d'induire l'activation de la MMP-9, a produit le même résultat dans ce systéme. L'addition de l'héparine dans nos experiences a inhibé l'activation de l'expression de MMP-9 suggerant que les heparanes sulfates dans syndecan-1 sont impliqué au mécanisme. Pour confirmer ces résultats des experiences avec des séries de syndecan-1 mutés sont en cours. Pour conclure, nos résultats montrent pour la première fois un rôle important de syndecan-1 à l'expression de MMP-9 suggérant que sa re-distribution au front des kératinocytes migratoires puisse éventuellement être liée au clivage ou à la dégradation des protéines de la matrice extracellulaire. En plus, nos résultats proposent que le domain LG4/5 de la laminin 332 libéré soit capable d'affecter la balance de l'expression de la MMP-9 lors de la migration… Advisors/Committee Members: Rousselle, Patricia (thesis director).

Subjects/Keywords: MMP-9; Syndecan-1; Keratinocyte; Laminin 332; MMP-9; Syndecan-1; Keratinocyte; Laminin 332; 571.6

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Michopoulou, A. (2016). Receptor syndecan-1 controls MMP-9 expression during keratinocyte migration : Le récepteur syndecan-1 contrôle l'expression de MMP-9 au cours de la migration des kératinocytes. (Doctoral Dissertation). Lyon. Retrieved from http://www.theses.fr/2016LYSE1130

Chicago Manual of Style (16^th Edition):

Michopoulou, Anna. “Receptor syndecan-1 controls MMP-9 expression during keratinocyte migration : Le récepteur syndecan-1 contrôle l'expression de MMP-9 au cours de la migration des kératinocytes.” 2016. Doctoral Dissertation, Lyon. Accessed March 09, 2021. http://www.theses.fr/2016LYSE1130.

MLA Handbook (7^th Edition):

Michopoulou, Anna. “Receptor syndecan-1 controls MMP-9 expression during keratinocyte migration : Le récepteur syndecan-1 contrôle l'expression de MMP-9 au cours de la migration des kératinocytes.” 2016. Web. 09 Mar 2021.

Vancouver:

Michopoulou A. Receptor syndecan-1 controls MMP-9 expression during keratinocyte migration : Le récepteur syndecan-1 contrôle l'expression de MMP-9 au cours de la migration des kératinocytes. [Internet] [Doctoral dissertation]. Lyon; 2016. [cited 2021 Mar 09]. Available from: http://www.theses.fr/2016LYSE1130.

Council of Science Editors:

Michopoulou A. Receptor syndecan-1 controls MMP-9 expression during keratinocyte migration : Le récepteur syndecan-1 contrôle l'expression de MMP-9 au cours de la migration des kératinocytes. [Doctoral Dissertation]. Lyon; 2016. Available from: http://www.theses.fr/2016LYSE1130

University of Toronto

10. Hanania, Raed. Investigation of the Production, Distribution, and Trafficking of MMP-9 in Classically Activated Macrophages.

Degree: 2012, University of Toronto

URL: http://hdl.handle.net/1807/33690

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As major effector cells of the innate immune response, macrophages must adeptly migrate from blood to infected tissues. Endothelial transmigration is accomplished by matrix metalloproteinase… (more)

Subjects/Keywords: macrophages; MMP-9; microtubules; Macrophage activation; Matrix Metalloproteinase 9; Trafficking; 0379

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Hanania, R. (2012). Investigation of the Production, Distribution, and Trafficking of MMP-9 in Classically Activated Macrophages. (Masters Thesis). University of Toronto. Retrieved from http://hdl.handle.net/1807/33690

Chicago Manual of Style (16^th Edition):

Hanania, Raed. “Investigation of the Production, Distribution, and Trafficking of MMP-9 in Classically Activated Macrophages.” 2012. Masters Thesis, University of Toronto. Accessed March 09, 2021. http://hdl.handle.net/1807/33690.

MLA Handbook (7^th Edition):

Hanania, Raed. “Investigation of the Production, Distribution, and Trafficking of MMP-9 in Classically Activated Macrophages.” 2012. Web. 09 Mar 2021.

Vancouver:

Hanania R. Investigation of the Production, Distribution, and Trafficking of MMP-9 in Classically Activated Macrophages. [Internet] [Masters thesis]. University of Toronto; 2012. [cited 2021 Mar 09]. Available from: http://hdl.handle.net/1807/33690.

Council of Science Editors:

Hanania R. Investigation of the Production, Distribution, and Trafficking of MMP-9 in Classically Activated Macrophages. [Masters Thesis]. University of Toronto; 2012. Available from: http://hdl.handle.net/1807/33690

11. Lucia, Conrado Ingraci de. Análise quantitativa dos níveis de cálcio, Colagenase A e B durante o reparo ósseo em calvárias de ratos sob o modelo experimental de defeito ósseo.

Degree: Mestrado, Biologia Oral, 2013, University of São Paulo

URL: http://www.teses.usp.br/teses/disponiveis/58/58137/tde-03042013-163708/ ;

►

O osso é um tipo especializado de tecido com alto teor mineral e desempenha variadas funções no organismo, como a reserva de cálcio, proteção de… (more)

▼

O osso é um tipo especializado de tecido com alto teor mineral e desempenha variadas funções no organismo, como a reserva de cálcio, proteção de estruturas vitais e alavanca para a movimentação dos musculos. Constantemente o osso passa por processos de remodelação, o que mantém sua estrutura funcional e repara pequenas fraturas que ocorrem normalmente devido ao estresse do uso contínuo. O sistema de reparo funciona em perfeita sincronia mediante células que produzem os componentes ésseos e células que os reabsorvem permitindo a organização do tecido. Esse sistema de manuteção depende da interação entre estas células bem como dos sinais enviados por mediadores e moduladores. Varias proteínas funcionam como indutores de formação óssea, mas também no sentido de facilitar essa reconstrução. Dentre estas proteínas se encontram as BMPs, que possuem grande potencial osteoindutor, e MMPs, que atuam em diversas fases da construção e manutenção deste tecido. Particularmente a BMP-2 tem mostrado um potencial significativo em termos de indução e sua forma recombinante a rhBMP-2, produzida por engenharia recombinante, foi liberada para comercialização e utilizacao clínica. Quanto às MMPs, há importante função das MMP-2 e MMP-9 neste tecido. A primeira estruturando a matriz e modulando o processo de reabsorção nos processos inflamatórios inerentes ao reparo; a segunda atuando desde fases iniciais às tardias, produzida principalmente por osteoclastos e utilizada na remodelação do osso novo. Porém, esta capacidade de reparo do osso é limitada e defeitos ósseos de grande extensão exigem muito do organismo, podendo levar a um reparo que não se estrutura devidamente. Assim, várias técnicas foram propostas para estimular o desenvolvimento ósseo e a utilizacao de enxertos se mostrou eficaz para fornecer um arcabouço de crescimento, facilitando a implantacao do osso neofomado e protegendo o leito do defeito durante todo o extenso período de recuperação. O presente estudo enfocou três diferentes tipos de enxerto ósseo (autólogo, homólogo e heterólogo) e suas associações com a proteína rhBMP-2, verificando sob o aspecto bioquímico a relação de cada um com a quantidade de MMP-2 e MMP-9 em dois tempos de reparo diferentes. De maneira geral, verificou-se que no primeiro momento há maior produção de MMP-2 e os níveis de MMP-9 se mantém de forma relativamente constante nos dois tempos cirúrgicos. O enxerto autólogo apresenta melhores resultados, seguido dos obtidos no enxerto homólogo e heterólogo respectivamente, entretanto a adição de rhBMP-2 a estes enxertos não parece influenciar nos níveis de MMP-2 e MMP-9 nos dois períodos. A dosagem de cálcio revelou que se apresentavam mais mineralizados os grupos de enxerto autólogo e homólogo, os demais grupos além de apresentar menores niveis de cálcio, ainda decresceram nestes níveis no segundo período do experimento.

Bone is a special tissue with a high mineral content and performs various functions in the body, such as calcium reserves, protection of vital structures and muscles lever during the…

Advisors/Committee Members: Issa, João Paulo Mardegan.

Subjects/Keywords: bone; bone graft; calcium dosage; dosagem de cálcio; enxerto osseo; MMP-2; MMP-2; MMP-9; MMP-9; rhBMP-2; rhBMP-2; tecido osseo

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Lucia, C. I. d. (2013). Análise quantitativa dos níveis de cálcio, Colagenase A e B durante o reparo ósseo em calvárias de ratos sob o modelo experimental de defeito ósseo. (Masters Thesis). University of São Paulo. Retrieved from http://www.teses.usp.br/teses/disponiveis/58/58137/tde-03042013-163708/ ;

Chicago Manual of Style (16^th Edition):

Lucia, Conrado Ingraci de. “Análise quantitativa dos níveis de cálcio, Colagenase A e B durante o reparo ósseo em calvárias de ratos sob o modelo experimental de defeito ósseo.” 2013. Masters Thesis, University of São Paulo. Accessed March 09, 2021. http://www.teses.usp.br/teses/disponiveis/58/58137/tde-03042013-163708/ ;.

MLA Handbook (7^th Edition):

Lucia, Conrado Ingraci de. “Análise quantitativa dos níveis de cálcio, Colagenase A e B durante o reparo ósseo em calvárias de ratos sob o modelo experimental de defeito ósseo.” 2013. Web. 09 Mar 2021.

Vancouver:

Lucia CId. Análise quantitativa dos níveis de cálcio, Colagenase A e B durante o reparo ósseo em calvárias de ratos sob o modelo experimental de defeito ósseo. [Internet] [Masters thesis]. University of São Paulo; 2013. [cited 2021 Mar 09]. Available from: http://www.teses.usp.br/teses/disponiveis/58/58137/tde-03042013-163708/ ;.

Council of Science Editors:

Lucia CId. Análise quantitativa dos níveis de cálcio, Colagenase A e B durante o reparo ósseo em calvárias de ratos sob o modelo experimental de defeito ósseo. [Masters Thesis]. University of São Paulo; 2013. Available from: http://www.teses.usp.br/teses/disponiveis/58/58137/tde-03042013-163708/ ;

12. Mata, Karina Magalhães Alves da. Participação das Metaloproteinases 2 e 9 no desenvolvimento de aneurisma da aorta abdominal em ratos Wistar.

Degree: Mestrado, Patologia, 2008, University of São Paulo

URL: http://www.teses.usp.br/teses/disponiveis/17/17143/tde-18112010-110823/ ;

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A degradação da matriz extracelular e de proteínas da parede aórtica associada à inflamação é uma das principais características dos aneurismas da aorta abdominal (AAA).… (more)

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A degradação da matriz extracelular e de proteínas da parede aórtica associada à inflamação é uma das principais características dos aneurismas da aorta abdominal (AAA). O objetivo deste trabalho foi investigar a participação das metaloproteinases 2 e 9 na formação de AAAs, através de um modelo experimental inédito de indução de AAA em ratos Wistar, desencadeado por duas potenciais causas de secreção e ativação de MMP-2 e 9: alteração do fluxo sanguíneo e lesão vascular externa na aorta. A formação de aneurismas foi observada em 60%-70% dos animais, apresentando diâmetro de 7 a 8 vezes maior que o diâmetro normal da aorta. Histologicamente observou-se remodelamento, intensa resposta inflamatória, destruição maciça de fibras elásticas e aumento da síntese de colágeno na parede aórtica. A expressão de ambas as formas de MMP-2 foram observadas tanto nos AAAs como nos grupos controles, neste com menor atividade, entretanto a expressão da pró e da MMP-9 ativa foram encontradas apenas nos AAAs. Conclusão: Nossos resultados sugerem que tanto as MMP-2 quanto as MMP-9 apresentam importante papel no desenvolvimento de AAA e este novo modelo de indução de AAA, pode ajudar a elucidar os mecanismos que desencadeiam a secreção e ativação das MMP-2 e MMP-9 na formação de aneurismas.

Degradation of extracellular matrix and proteins associated with inflammation of the aortic wall is the main characteristics of the abdominal aortic aneurysms (AAA). The aim of this study was investigate the participation of Metalloproteinase 2 and 9 in AAA formation in Wistar rats. A novel experimental model of AAA was developed, providing two potential causes of MMPs secretion and activation, turbulent flow (caused by surgically induced extrinsic stenosis) and outside vascular injury is detailed described. The days analyzed were the 3rd and the 7th post surgery. Aneurysms were observed to occur in 60-70% of the Group AAA, exhibiting a major transversal diameter to 7 from 8 times larger than controls and sham groups. Histologically, the aneurysms wall showed extensive structural remodeling, intense inflammatory response, massive elastic fibers destruction and abundant collagen deposition. Increased pro- and active MMP-2 was demonstrated in the AAA and controls groups, whereas pro- and active MMP-9 were found to be expressed only in the AAA group. Conclusions: MMP-2 and MMP-9 may have a pivotal role in the development of experimental AAA. This model can help to elucidate the mechanisms which trigger off MMP-2 and MMP-9 secretion and activation causing aneurysms.

Advisors/Committee Members: Ramos, Simone Gusmão.

Subjects/Keywords: Aneurisma; Aneurysm; estenose; fluxo turbulento; injury; lesão vascular; MMP-2; MMP-2; MMP-9; MMP-9; remodelamento vascular; stenosis; turbulent flow; vascular remodeling

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Mata, K. M. A. d. (2008). Participação das Metaloproteinases 2 e 9 no desenvolvimento de aneurisma da aorta abdominal em ratos Wistar. (Masters Thesis). University of São Paulo. Retrieved from http://www.teses.usp.br/teses/disponiveis/17/17143/tde-18112010-110823/ ;

Chicago Manual of Style (16^th Edition):

Mata, Karina Magalhães Alves da. “Participação das Metaloproteinases 2 e 9 no desenvolvimento de aneurisma da aorta abdominal em ratos Wistar.” 2008. Masters Thesis, University of São Paulo. Accessed March 09, 2021. http://www.teses.usp.br/teses/disponiveis/17/17143/tde-18112010-110823/ ;.

MLA Handbook (7^th Edition):

Mata, Karina Magalhães Alves da. “Participação das Metaloproteinases 2 e 9 no desenvolvimento de aneurisma da aorta abdominal em ratos Wistar.” 2008. Web. 09 Mar 2021.

Vancouver:

Mata KMAd. Participação das Metaloproteinases 2 e 9 no desenvolvimento de aneurisma da aorta abdominal em ratos Wistar. [Internet] [Masters thesis]. University of São Paulo; 2008. [cited 2021 Mar 09]. Available from: http://www.teses.usp.br/teses/disponiveis/17/17143/tde-18112010-110823/ ;.

Council of Science Editors:

Mata KMAd. Participação das Metaloproteinases 2 e 9 no desenvolvimento de aneurisma da aorta abdominal em ratos Wistar. [Masters Thesis]. University of São Paulo; 2008. Available from: http://www.teses.usp.br/teses/disponiveis/17/17143/tde-18112010-110823/ ;

13. Sougleri, Ioanna. Συμβολή λοιμοτοξικών παραγόντων του ελικοβακτηριδίου του πυλωρού στην ενεργοποίηση παραγόντων που ρυθμίζουν τη δομή της εξωκυττάριας ύλης.

Degree: 2018, National and Kapodistrian University of Athens; Εθνικό και Καποδιστριακό Πανεπιστήμιο Αθηνών (ΕΚΠΑ)

URL: http://hdl.handle.net/10442/hedi/43664

►

As a result of H. pylori adhesion to gastric epithelial cells the bacterial effector CagA is translocated intracellularly and, following its hierarchic tyrosine phosphorylation on… (more)

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As a result of H. pylori adhesion to gastric epithelial cells the bacterial effector CagA is translocated intracellularly and, following its hierarchic tyrosine phosphorylation on multiple EPIYA motifs, deregulates cellular polarity and contributes to the induction of an elongation and scattering phenotype resembling epithelial to mesenchymal transition (EMT). Stromelysin-1/matrix metalloproteinase-3 (MMP-3) has been reported to induce a sequence of molecular alterations leading to stable EMT conversion and carcinogenesis in epithelial cells. To identify the putative role of CagA protein in MMP-3 induction we exploited an experimental H. pylori infection system in gastric epithelial cell lines. We utilized isogenic mutants expressing CagA protein with variable numbers of terminal EPIYA and phosphorylation-deficient EPIFA motifs, as well as the cagA-KO and translocation-deficient cagE-KO strains. Increased levels of MMP-3 transcriptional activation was determined by RTqPCR, in the case of strains bearing more than 2 terminal EPIYA phosphorylation motifs in CagA. MMP-3 expression in total cell lysates and the corresponding culture supernatants was associated to CagA expression, translocation and was dependent on CagA phosphorylation. CagA-EPIYA phosphorylation-dependent increase in gelatinase and caseinolytic activity was also detected in culture supernatants, utilizing zymography. Significant increase in transcriptional activity of mesenchymal markers vimentin, Snail and ZEB1 and stem cell marker CD44, was observed only in the case of EPIYA phosphorylation-functional CagA. CagA EPIYA phosphorylationdependent increase in the transcriptional activation of MMP-9, another EMT marker, was also observed. Our data suggest that CagA protein can induce EMT through its successful intracellular translocation and subsequent phosphorylation, by a mechanism which also involves MMP-3, as well as, key mesenchymal and stemness markers Vimentin, Snail, ZEB1, and CD44.

Μετά τη προσκόλληση του Helicobacter pylori (H. pylori) στα γαστρικά επιθηλιακά κύτταρα, η πρωτεΐνη CagA μεταφέρεται ενδοκυτταρικά στα γαστρικά επιθηλιακά κύτταρα και ακολουθεί η διαδοχική της φωσφορυλίωση σε κατάλοιπα τυροσίνης πολλαπλών μοτίβων EPIYA. Η φωσφορυλίωση των μοτίβων συντελεί στην απορρύθμιση της κυτταρικής πολικότητας των επιθηλιακών κυττάρων, καθώς και στην απόκτηση ενός φαινότυπου διασποράς και επιμήκυνσης, ο οποίος προσομοιάζει στην επιθηλιακή προς μεσεγχυματική μετατροπή (EMT). Η στρωμελυσίνη-1/ιστική μεταλλοπρωτεϊνάση-3 (MMP-3) έχει δειχθεί ότι επάγει μια ακολουθία μοριακών μεταβολών οι οποίες οδηγούν στην EMT και στη καρκινογένεση των επιθηλιακών κυττάρων. Για να προσδιορίσουμε το ρόλο της πρωτεΐνης CagA κατά την επαγωγή της MMP-3 χρησιμοποιήσαμε ένα πειραματικό σύστημα, όπου μολύνθηκαν γαστρικές επιθηλιακές σειρές με ποικίλα στελέχη του H. pylori. Πιο συγκεκριμένα, χρησιμοποιήσαμε ισογενή μεταλλαγμένα στελέχη, τα οποία εξέφραζαν τη πρωτεΐνη CagA με μεταβαλόμενο αριθμό λειτουργικών θέσεων φωσφορυλίωσης EPIYA, καθώς επίσης και τα αντίστοιχα μη…

Subjects/Keywords: Μεταλλοπρωτεϊνάσες του ιστού; Στρωμελυσίνη-1/MMP-3; MMP-9; Βιμεντίνη; Snai1; ZEB1; Υποδοχέας CD44; Matrix metalloproteinase; stromelysin-1/MMP-3; MMP-9; Vimentin; Snai1; ZEB1; CD44

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Sougleri, I. (2018). Συμβολή λοιμοτοξικών παραγόντων του ελικοβακτηριδίου του πυλωρού στην ενεργοποίηση παραγόντων που ρυθμίζουν τη δομή της εξωκυττάριας ύλης. (Thesis). National and Kapodistrian University of Athens; Εθνικό και Καποδιστριακό Πανεπιστήμιο Αθηνών (ΕΚΠΑ). Retrieved from http://hdl.handle.net/10442/hedi/43664

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Sougleri, Ioanna. “Συμβολή λοιμοτοξικών παραγόντων του ελικοβακτηριδίου του πυλωρού στην ενεργοποίηση παραγόντων που ρυθμίζουν τη δομή της εξωκυττάριας ύλης.” 2018. Thesis, National and Kapodistrian University of Athens; Εθνικό και Καποδιστριακό Πανεπιστήμιο Αθηνών (ΕΚΠΑ). Accessed March 09, 2021. http://hdl.handle.net/10442/hedi/43664.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Sougleri, Ioanna. “Συμβολή λοιμοτοξικών παραγόντων του ελικοβακτηριδίου του πυλωρού στην ενεργοποίηση παραγόντων που ρυθμίζουν τη δομή της εξωκυττάριας ύλης.” 2018. Web. 09 Mar 2021.

Vancouver:

Sougleri I. Συμβολή λοιμοτοξικών παραγόντων του ελικοβακτηριδίου του πυλωρού στην ενεργοποίηση παραγόντων που ρυθμίζουν τη δομή της εξωκυττάριας ύλης. [Internet] [Thesis]. National and Kapodistrian University of Athens; Εθνικό και Καποδιστριακό Πανεπιστήμιο Αθηνών (ΕΚΠΑ); 2018. [cited 2021 Mar 09]. Available from: http://hdl.handle.net/10442/hedi/43664.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Sougleri I. Συμβολή λοιμοτοξικών παραγόντων του ελικοβακτηριδίου του πυλωρού στην ενεργοποίηση παραγόντων που ρυθμίζουν τη δομή της εξωκυττάριας ύλης. [Thesis]. National and Kapodistrian University of Athens; Εθνικό και Καποδιστριακό Πανεπιστήμιο Αθηνών (ΕΚΠΑ); 2018. Available from: http://hdl.handle.net/10442/hedi/43664

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Universidade de Lisboa

14. Veríssimo, Eduarda Pimenta. Aloe species in colorectal cancer therapy: friend or foe?.

Degree: 2016, Universidade de Lisboa

URL: http://www.rcaap.pt/detail.jsp?id=oai:repositorio.ul.pt:10451/26434

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Tese de mestrado, Biologia Molecular e Genética, Universidade de Lisboa, Faculdade de Ciências, 2016

Aloe plants have been suggested to be an important natural source… (more)

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Tese de mestrado, Biologia Molecular e Genética, Universidade de Lisboa, Faculdade de Ciências, 2016

Aloe plants have been suggested to be an important natural source of medical therapy agents (including for cancer) for several years. Throughout the world, pharmaceutical companies and institutions are struggling to isolate its most potent bioactive compounds as a primary means of using its activities. Regardless of the species used, the extraction procedure selected or the lack of specific compounds responsible for any given bioactivity, the fact is that we have been witnessing several debates, disputes and a lot of conflicting results arising on the claimed anticancer properties of Aloe species. During the last decade, several reports demonstrated that a subgroup of matrix metalloproteinases (MMPs) called gelatinases (MMP-2 and especially MMP-9), are largely responsible for colorectal cancer progression/metastasis, suggesting that MMP inhibitors (MMPIs) may be a powerful tool to reduce cancer invasion. Although Aloe’s activities suggest they might inhibit MMPs, no studies have related their reported anticancer activity with MMPI activities. Also, although approximately 500 species have been identified so far within the Aloe genus, A. vera is the most widely studied albeit other species such as A. arborescens have also been reported to exhibit similar bioactivities. Hence, the goal of the present study was to compare the anticancer potential of two Aloe species, A. vera and A. arborescens and to ascertain if they are specific MMP-9 and/or MMP-2 inhibitors. Different types of extraction were tested (100% (v/v) methanol, 50% (v/v) methanol and 100 mM Tris-HCl buffer pH 7) and specific bioactive compounds (proteins, total phenolic compounds, anthraquinones and total carbohydrates) were quantified and compared. Although there were a few variations between species and between extractions, 50% (v/v) methanol was selected as the best extraction procedure. Anticancer activities were measured in vitro using the wound healing model assay in the human colon cancer cells HT29, and A. vera showed significantly higher inhibitory potencial regarding wound closure. Furthermore, it was also evaluated their effects on gelatinase activities, measured by gelatin zymography and the DQ gelatinase assay. When assessing the total gelatinolytic activity, both species had a similar result of around 20% inhibition. Through the gelatin zimography we demonstrated that both MMP-9 pro-form and active form were inhibited, but only the MMP-2 pro form were inhibited, which corroborates the results obtained with the total gelatinolytic activity assay. The gelatinolytic activity of MMP-9 showed that although both species have the potencial to inhibit specifically this metalloproteinase, A. arborescens have a significantly higher inhibitory effectiveness. Overall, our data provided clear indication that A. arborescens and A. vera have indeed potential as inhibitory agents in cancer therapy, being able to reduce colon cancer cell proliferation and…

Advisors/Committee Members: Ferreira, Ricardo Boavida, 1957-, Silva, Anabela Rosa Bernardes dos Santos da, 1960-.

Subjects/Keywords: Aloe; Cancro; MMP-9; MMP-2; HT29; Teses de mestrado - 2016; Departamento de Biologia Vegetal

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Veríssimo, E. P. (2016). Aloe species in colorectal cancer therapy: friend or foe?. (Thesis). Universidade de Lisboa. Retrieved from http://www.rcaap.pt/detail.jsp?id=oai:repositorio.ul.pt:10451/26434

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Veríssimo, Eduarda Pimenta. “Aloe species in colorectal cancer therapy: friend or foe?.” 2016. Thesis, Universidade de Lisboa. Accessed March 09, 2021. http://www.rcaap.pt/detail.jsp?id=oai:repositorio.ul.pt:10451/26434.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Veríssimo, Eduarda Pimenta. “Aloe species in colorectal cancer therapy: friend or foe?.” 2016. Web. 09 Mar 2021.

Vancouver:

Veríssimo EP. Aloe species in colorectal cancer therapy: friend or foe?. [Internet] [Thesis]. Universidade de Lisboa; 2016. [cited 2021 Mar 09]. Available from: http://www.rcaap.pt/detail.jsp?id=oai:repositorio.ul.pt:10451/26434.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Veríssimo EP. Aloe species in colorectal cancer therapy: friend or foe?. [Thesis]. Universidade de Lisboa; 2016. Available from: http://www.rcaap.pt/detail.jsp?id=oai:repositorio.ul.pt:10451/26434

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

15. Καψάλη, Αναστασία. Βιολογικές δράσεις ενός συνθετικού πεπτιδίου του αυξητικού παράγοντα HARP.

Degree: 2010, University of Patras

URL: http://nemertes.lis.upatras.gr/jspui/handle/10889/4511

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Η HARP (Heparin Affin Regulatory Peptide) είναι ένας αυξητικός παράγοντας με Μ.Β. 18 kDa που ανήκει στην οικογένεια των αυξητικών παραγόντων που έχουν συγγένεια με… (more)

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Η HARP (Heparin Affin Regulatory Peptide) είναι ένας αυξητικός παράγοντας με Μ.Β. 18 kDa που ανήκει στην οικογένεια των αυξητικών παραγόντων που έχουν συγγένεια με την ηπαρίνη. Eμπλέκεται στην ανάπτυξη των νευριτών, την επούλωση πληγών και φαίνεται να παίζει σημαντικό επαγωγικό ρόλο στις διαδικασίες της ογκογένεσης, καθώς επάγει την αγγειογένεση και εμφανίζεται σε υψηλές συγκεντρώσεις τόσο σε καρκινικούς ιστούς, όσο και σε κυτταρικές σειρές καρκινικών κυττάρων. Στο πλαίσιο μελέτης της σχέσης δομής/δράσης του αυξητικού αυτού παράγοντα, χρησιμοποιούνται τόσο συνθετικά πεπτίδια, όσο και ανασυνδυασμένες τροποποιημένες μορφές του αυξητικού αυτού παράγοντα. Σε φυσιολογικές συνθήκες, η εκκρινόμμενη HARP πέπτεται από ένζυμα του κυτταρικού μικροπεριβάλλοντος και προκύπτουν πεπτίδια που παρουσιάζουν βιολογικές δράσεις παρόμοιες ή και αντίθετες από αυτές της HARP. Φαίνεται λοιπόν πως η δράση του αυξητικού αυτού παράγοντα ρυθμίζεται τόσο στο επίπεδο βιοσύνθεσης και έκκρισης, όσο και από τη δράση ενζύμων του εξωκυττάριου χώρου. Στην παρούσα εργασία μελετήθηκε η δράση ενός συνθετικού πεπτιδίου το οποίο αντιστοιχεί στα αμινοξέα 65-97 που εντοπίζονται στην ΤSR περιοχή προς το καρβοξυτελικό άκρο της HARP. Με δεδομένο ότι τι πεπτίδιο αυτό εμφανίζει αντιαγγειογενετική δράση, πραγματοποιήθηκαν χρονοεξαρτώμενα και δοσοεξαρτώμενα πειράματα, με σκοπό τη μελέτη της δράσης του στον πολλαπλασιασμό, τη μετανάστευση και την επούλωση πληγών. Στο πλαίσιο αυτών των μελετών, ελέγξαμε τη δράση του στην έκφραση των μεταλλοπρωτεϊνασών ΜΜP-2 και ΜΜP-9, των αναστολέων τους ΤΙMP-1 και ΤΙMP-2 καθώς και του κολλαγόνου και της ελαστίνης σε πρωτογενείς καλλιέργειες ενδοθηλιακών κυττάρων από ομφάλιο λώρο (HUVEC cells). Τα αποτελέσματα έδειξαν πως το συνθετικό αυτό πεπτίδιο καταστέλλει τον πολλαπλασιασμό, την μετανάστευση αλλά και την επούλωση πλήγών των κυττάρων HUVEC με δοδοεξαρτώμενο και στατιστικώς σημαντικό τρόπο. Επιπλέον από τα πειράματά μας δεν παρατηρήθηκε μεταβολή στα πρωτεϊνικά επίπεδα έκφρασης των μεταλλοπρωτεϊνασών ΜΜP-2 και ΜΜP-9 καθώς και των αναστολέων τους ΤΙMP-1 και ΤΙMP-2. Ωστόσο, παρατηρήθηκε στατιστικώς σημαντική μεταβολή στα επίπεδα γονιδιακής έκφρασης των αναστολέων ΤΙMP-1 και ΤΙMP-2 όπως επίσης και της ελαστίνης και του κολλαγόνου IV.

Heparin affin regulatory peptide (HARP) is an 18-kDa secreted growth factor that has a high affinity for heparin and a potent role on tumor growth and angiogenesis. HARP was originally described as a neurite outgrowth promoting molecule, which appears to increases during recovery from injury and is thought to be involved in angiogenesis expression, playing a major role in the cell growth and differentiation that are associated with regeneration in several tissues. HARP is expressed in several human tumors and tumor cell lines and is also indicated in high serum levels of patients with different types of cancer. HARP contains two random coiled clusters of basic residues (N- and C-terminal) and two b-sheet domain. Each b-sheet domain contains a thrombospondin repeat I (TSR-I) motif, which have…

Advisors/Committee Members: Κατσώρης, Παναγιώτης, Kapsali, Anastasia, Φλυτζάνης, Κωνσταντίνος, Μίντζας, Αναστάσιος, Κατσώρης, Παναγιώτης.

Subjects/Keywords: Πεπτίδια; Ελαστίνη; 572.65; HΑRP; TIMP-1; TIMP-2; MMP-2; MMP-9; Elastin

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Καψάλη, �. (2010). Βιολογικές δράσεις ενός συνθετικού πεπτιδίου του αυξητικού παράγοντα HARP. (Masters Thesis). University of Patras. Retrieved from http://nemertes.lis.upatras.gr/jspui/handle/10889/4511

Chicago Manual of Style (16^th Edition):

Καψάλη, Αναστασία. “Βιολογικές δράσεις ενός συνθετικού πεπτιδίου του αυξητικού παράγοντα HARP.” 2010. Masters Thesis, University of Patras. Accessed March 09, 2021. http://nemertes.lis.upatras.gr/jspui/handle/10889/4511.

MLA Handbook (7^th Edition):

Καψάλη, Αναστασία. “Βιολογικές δράσεις ενός συνθετικού πεπτιδίου του αυξητικού παράγοντα HARP.” 2010. Web. 09 Mar 2021.

Vancouver:

Καψάλη �. Βιολογικές δράσεις ενός συνθετικού πεπτιδίου του αυξητικού παράγοντα HARP. [Internet] [Masters thesis]. University of Patras; 2010. [cited 2021 Mar 09]. Available from: http://nemertes.lis.upatras.gr/jspui/handle/10889/4511.

Council of Science Editors:

Καψάλη �. Βιολογικές δράσεις ενός συνθετικού πεπτιδίου του αυξητικού παράγοντα HARP. [Masters Thesis]. University of Patras; 2010. Available from: http://nemertes.lis.upatras.gr/jspui/handle/10889/4511

Brigham Young University

16. Schuler, Jeffrey Thomas. Forward Chemical Genetics Drug Screen Yields Novel Proteases and Proteolytic Inhibitors of HGF–induced Epithelial–Mesenchymal Transition.

Degree: MS, 2016, Brigham Young University

URL: https://scholarsarchive.byu.edu/cgi/viewcontent.cgi?article=7257&context=etd

► Hepatocyte Growth Factor (HGF)–induced Epithelial–Mesenchymal Transition (EMT) is a complex cellular pathway that causes epithelial cell scattering by breaking cell–cell contacts, eliminating apical–basal polarity, and… (more)

Subjects/Keywords: EMT; cell spreading; cell compaction; HGF; BACE; EphA2; Furin; MMP–9; MMP–12; Physiology

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Schuler, J. T. (2016). Forward Chemical Genetics Drug Screen Yields Novel Proteases and Proteolytic Inhibitors of HGF–induced Epithelial–Mesenchymal Transition. (Masters Thesis). Brigham Young University. Retrieved from https://scholarsarchive.byu.edu/cgi/viewcontent.cgi?article=7257&context=etd

Chicago Manual of Style (16^th Edition):

Schuler, Jeffrey Thomas. “Forward Chemical Genetics Drug Screen Yields Novel Proteases and Proteolytic Inhibitors of HGF–induced Epithelial–Mesenchymal Transition.” 2016. Masters Thesis, Brigham Young University. Accessed March 09, 2021. https://scholarsarchive.byu.edu/cgi/viewcontent.cgi?article=7257&context=etd.

MLA Handbook (7^th Edition):

Schuler, Jeffrey Thomas. “Forward Chemical Genetics Drug Screen Yields Novel Proteases and Proteolytic Inhibitors of HGF–induced Epithelial–Mesenchymal Transition.” 2016. Web. 09 Mar 2021.

Vancouver:

Schuler JT. Forward Chemical Genetics Drug Screen Yields Novel Proteases and Proteolytic Inhibitors of HGF–induced Epithelial–Mesenchymal Transition. [Internet] [Masters thesis]. Brigham Young University; 2016. [cited 2021 Mar 09]. Available from: https://scholarsarchive.byu.edu/cgi/viewcontent.cgi?article=7257&context=etd.

Council of Science Editors:

Schuler JT. Forward Chemical Genetics Drug Screen Yields Novel Proteases and Proteolytic Inhibitors of HGF–induced Epithelial–Mesenchymal Transition. [Masters Thesis]. Brigham Young University; 2016. Available from: https://scholarsarchive.byu.edu/cgi/viewcontent.cgi?article=7257&context=etd

Univerzitet u Beogradu

17. Radunović, Milena, 1984-. The role of Cytomegalovirus and Epstein-Barr virus in the development and progression of Salivary Gland Cancer.

Degree: Medicinski fakultet, 2017, Univerzitet u Beogradu

URL: https://fedorabg.bg.ac.rs/fedora/get/o:14282/bdef:Content/get

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Molecular medicine - Microbiology / Molekularna medicina - Mikrobiologija

Introduction: Salivary gland carcinomas (SGC) are rare tumors characterized by an enormous morphological diversity between different… (more)

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Molecular medicine - Microbiology / Molekularna medicina - Mikrobiologija

Introduction: Salivary gland carcinomas (SGC) are rare tumors characterized by an enormous morphological diversity between different subtypes going along with diverse clinical courses. The etiology of SGC is still unknown, although a correlation has been shown between the occurrence of SGC and some environmental factors, however, only a very small percentage of malignancies develop as a direct result of these factors. There is growing evidence that infectious agents are frequently associated with human cancer. Recent studies show that Cytomegalovirus (CMV) and Epstein-Barr virus (EBV) CMV infections are associated with various epithelial malignancies. It has been shown that in various tissues CMV and EBV could increase the expression of IL-6, NFκB, MMP-2 and MMP-9. These proteins are associated with certain types of cancer. We hypothesized that CMV and EBV could cause inflammation and deregulation of genes involved in cell proliferation and could induce the development of salivary gland cancer. Aims: The aims of this study were to determine the prevalence of CMV and EBV in SGC tissues, to compare the immunohistochemical and nested PCR detection of CMV and EBV in SGC, to investigate the viral protein expression in different histological types of salivary gland cancer, to investigate the immunohistochemical expression of prognostic markers IL6, MMP2, MMP9 and NFκB, to investigate the association of the presence of CMV and EBV and the immunohistochemical expression of these prognostic markers and to analyze the polymorphisms of IL6, MMP2 and MMP9 genes and investigate the association of the polymorphisms and the corresponding protein expression. Material and Methods: This cross-section study included 93 patients diagnosed with salivary gland cancer (SGC), surgically treated at the Clinic of Otorhinolaryngology and Maxillofacial Surgery, Clinical Center of Serbia from 2004 to 2013. For the immunohistochemical analysis, the control consisted of healthy salivary gland tissue from 20 autopsy cases with no malignancies and salivary gland pathology...

Advisors/Committee Members: Novaković, Ivana, 1961-.

Subjects/Keywords: Citomegalovirus; Epštajn-Bar virus; karcinom pljuvačnih žlezda; IL-6; NFκB; MMP-2; MMP-9

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Radunović, Milena, 1. (2017). The role of Cytomegalovirus and Epstein-Barr virus in the development and progression of Salivary Gland Cancer. (Thesis). Univerzitet u Beogradu. Retrieved from https://fedorabg.bg.ac.rs/fedora/get/o:14282/bdef:Content/get

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Radunović, Milena, 1984-. “The role of Cytomegalovirus and Epstein-Barr virus in the development and progression of Salivary Gland Cancer.” 2017. Thesis, Univerzitet u Beogradu. Accessed March 09, 2021. https://fedorabg.bg.ac.rs/fedora/get/o:14282/bdef:Content/get.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Radunović, Milena, 1984-. “The role of Cytomegalovirus and Epstein-Barr virus in the development and progression of Salivary Gland Cancer.” 2017. Web. 09 Mar 2021.

Vancouver:

Radunović, Milena 1. The role of Cytomegalovirus and Epstein-Barr virus in the development and progression of Salivary Gland Cancer. [Internet] [Thesis]. Univerzitet u Beogradu; 2017. [cited 2021 Mar 09]. Available from: https://fedorabg.bg.ac.rs/fedora/get/o:14282/bdef:Content/get.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Radunović, Milena 1. The role of Cytomegalovirus and Epstein-Barr virus in the development and progression of Salivary Gland Cancer. [Thesis]. Univerzitet u Beogradu; 2017. Available from: https://fedorabg.bg.ac.rs/fedora/get/o:14282/bdef:Content/get

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of South Carolina

18. Speiran, Kelly Lauren. The Gelatinases and Autoimmune Disease.

Degree: MS, Biomedical Science, 2011, University of South Carolina

URL: https://scholarcommons.sc.edu/etd/2121

► Matrix metalloproteases (MMPs) are critical to an array of homeostatic processes but have also been implicated in many pathological states. The roles they play… (more)

▼ Matrix metalloproteases (MMPs) are critical to an array of homeostatic processes but have also been implicated in many pathological states. The roles they play in innate immunity, regulation of physical barriers such as the blood brain barrier, and the healing process are the same ones that contribute to their role in autoimmune disease. While MMPs are best known for being dysregulated in chronic disease states, they also have the ability to contribute to disease onset. There are highly specific regulatory differences among MMPs. This is how the proper MMP is transcribed, translated, and activated for a given situation. While some MMPs appear redundant, there are critical differences in each MMP's regulatory controls. These controls are involved in regulation of MMPs and may be easily disrupted through endogenous and exogenous factors. As such, the carefully orchestrated MMP cascade can also become misregulated and destructive. When the wrong MMP is activated or an MMP is expressed in the wrong amount, place, or time, this can result in damage or disease. These aspects of MMP regulation are part of what confers their ability to initiate, promote, and maintain autoimmune disease. The review illustrates the ways in which two MMPs, MMP-2 and MMP-9 of the gelatinase family, appear similar but are differentially regulated, have varied actions, and how they have different expression profiles in disease. First I will highlight how these MMPs are regulated. Subsequently, I will present how environmental exposures and hormonal influences can also influence these MMPs. Each way in which MMP levels can be altered has implications in autoimmune disease. The main goal of highlighting endogenous and exogenous regulators of MMPs is to illustrate the points of regulation in which MMPs may contribute to autoimmune disease. These are points that can also potentially be used for therapeutic intervention. I will also review several autoimmune diseases in which MMPs play multiple roles in pathology. The discussion of autoimmune diseases will cover autoimmune arthritic diseases (which include rheumatoid arthritis (RA), psoriatic arthritis, and juvenile idiopathic arthritis (JIA)), systemic lupus erythematosus (SLE), and multiple sclerosis (MS). Advisors/Committee Members: Jennifer Nyland.

Subjects/Keywords: Biomedical; Chemicals and Drugs; Medicine and Health Sciences; autoimmune disease; gelatinases; MMP-2; MMP-9

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Speiran, K. L. (2011). The Gelatinases and Autoimmune Disease. (Masters Thesis). University of South Carolina. Retrieved from https://scholarcommons.sc.edu/etd/2121

Chicago Manual of Style (16^th Edition):

Speiran, Kelly Lauren. “The Gelatinases and Autoimmune Disease.” 2011. Masters Thesis, University of South Carolina. Accessed March 09, 2021. https://scholarcommons.sc.edu/etd/2121.

MLA Handbook (7^th Edition):

Speiran, Kelly Lauren. “The Gelatinases and Autoimmune Disease.” 2011. Web. 09 Mar 2021.

Vancouver:

Speiran KL. The Gelatinases and Autoimmune Disease. [Internet] [Masters thesis]. University of South Carolina; 2011. [cited 2021 Mar 09]. Available from: https://scholarcommons.sc.edu/etd/2121.

Council of Science Editors:

Speiran KL. The Gelatinases and Autoimmune Disease. [Masters Thesis]. University of South Carolina; 2011. Available from: https://scholarcommons.sc.edu/etd/2121

19. Φωτόπουλος, Βασίλειος. Σηπτική αρθρίτις γόνατος: διάγνωση και αντιμετώπιση: κλινική σημασία των ζελατινασών, MMP-2 και MMP-9 στη σηπτική αρθρίτιδα του γόνατος.

Degree: 2012, University of Ioannina; Πανεπιστήμιο Ιωαννίνων

URL: http://hdl.handle.net/10442/hedi/28460

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Purpose: In cases of septic knee arthritis there is excess of matrix metalloproteinases (MMPs) over tissue inhibitors of metalloproteinases (TIMPs), due to enhanced expression and… (more)

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Purpose: In cases of septic knee arthritis there is excess of matrix metalloproteinases (MMPs) over tissue inhibitors of metalloproteinases (TIMPs), due to enhanced expression and activation which are induced by bacteria in comparison to rheumatic or degenerative arthritis. The aim of this study was to explore the expression levels of synovial gelatinase MMP-9 and its specific inhibitor TIMP-1 in septic and aseptic arthritis and their potential use as additional aids to clinical investigation. Methods: Gelatin zymography and western blot analysis were applied in effusions from knees of patients with septic (SA/10 patients), rheumatic (RA/10 patients) and osteoarthritis (OA/10 patients). Results: Zymographic analysis revealed that all samples contained latent MMP-2 activity, albeit activated MMP-2 appeared in more of the septic than aseptic effusions. Activated MMP-9 was not detected in osteoarthritic synovial fluid samples. Only trace amounts of MMP-9 activity were detected in 4/10 patients with RA, whereas higher MMP-9 levels were evident in all samples from SA (P=0.0444). In immunoblotting assays, samples from SA showed significantly higher levels of MMP-9 compared to samples from RA (P=0.0052), confirming zymographic results. Although no significant difference in TIMP-1 levels was observed, the estimated MMP-9/TIMP-1 ratio of septic effusions was significantly higher compared to aseptic ones (P=0.0029). Conclusions: The data presented suggest enhanced expression and activation of MMP-9 in septic native knee arthritis compared to aseptic. The presence of high levels of MMP-9 with concomitantly increased MMP-9/TIMP-1 ratio and activated gelatinases in effusions, independent of neutrophilic counts, may be indicative for infection.

Σκοπός: Σε περίπτωση σηπτικής αρθρίτιδας του γόνατος, παρατηρείται υπεροχή των στρωματικών μεταλλοπρωτεϊνασών (MMPs) σε βάρος των ιστικών αναστολέων τους (TIMPs), εξαιτίας αυξημένης, επαγόμενης από βακτήρια, έκφρασης και ενεργοποίησης αυτών σε σύγκριση με την αυτοάνοσης αρχής ή εκφυλιστικού τύπου αρθρίτιδα. Ο σκοπός της εργασίας αυτής είναι ο προσδιορισμός των επιπέδων έκφρασης της ζελατινάσης MMP-9 και του ειδικού αναστολέα της, TIMP-1, σε αρθρικό υγρό από πάσχουσες αρθρώσεις και η διερεύνηση της πιθανότητας χρήσης τους ως διαγνωστικού κριτηρίου κατά την προσέγγιση της οξείας μονοαρθρίτιδας του γόνατος. Υλικό & Μέθοδος: Ζυμογραφία ζελατίνης και ανάλυση western blot πραγματοποιήθηκε σε δείγματα αρθρικού υγρού από γόνατα ασθενών πασχόντων από σηπτική (SA/10 ασθενείς), ρευματική (RA/10 ασθενείς) και οστεοαρθρίτιδα (OA/10 ασθενείς). Αποτελέσματα: Η ζυμογραφία αποκάλυψε έκφραση proΜΜΡ-2 σε όλα τα δείγματα αρθρικού υγρού, ενώ η ενεργοποιημένη μορφή της ήταν παρούσα σε περισσότερα σηπτικά. ΜΜΡ-9 δεν ανιχνεύθηκε σε οστεοαρθριτιδικά δείγματα. Ίχνη μόνον ΜΜΡ-9 δράσης ανιχνεύθηκε σε 4/10 ασθενείς με RA, ενώ υψηλότερα επίπεδα ΜΜΡ-9 πιστοποιήθηκαν σε όλα τα σηπτικά δείγματα (Ρ=0.0444). Στις δοκιμασίες ανοσοαποτύπωσης, δείγματα από ασθενείς με SA επέδειξαν σημαντικά υψηλότερα επίπεδα ΜΜΡ-9 σε…

Subjects/Keywords: Σηπτική αρθρίτιδα; Γόνατα; Ζελατινάσες; Septic arthritis; Knee; Gelatinases; MMP-2; MMP-9; TIMP-1

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Φωτόπουλος, . �. (2012). Σηπτική αρθρίτις γόνατος: διάγνωση και αντιμετώπιση: κλινική σημασία των ζελατινασών, MMP-2 και MMP-9 στη σηπτική αρθρίτιδα του γόνατος. (Thesis). University of Ioannina; Πανεπιστήμιο Ιωαννίνων. Retrieved from http://hdl.handle.net/10442/hedi/28460

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Φωτόπουλος, Βασίλειος. “Σηπτική αρθρίτις γόνατος: διάγνωση και αντιμετώπιση: κλινική σημασία των ζελατινασών, MMP-2 και MMP-9 στη σηπτική αρθρίτιδα του γόνατος.” 2012. Thesis, University of Ioannina; Πανεπιστήμιο Ιωαννίνων. Accessed March 09, 2021. http://hdl.handle.net/10442/hedi/28460.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Φωτόπουλος, Βασίλειος. “Σηπτική αρθρίτις γόνατος: διάγνωση και αντιμετώπιση: κλινική σημασία των ζελατινασών, MMP-2 και MMP-9 στη σηπτική αρθρίτιδα του γόνατος.” 2012. Web. 09 Mar 2021.

Vancouver:

Φωτόπουλος �. Σηπτική αρθρίτις γόνατος: διάγνωση και αντιμετώπιση: κλινική σημασία των ζελατινασών, MMP-2 και MMP-9 στη σηπτική αρθρίτιδα του γόνατος. [Internet] [Thesis]. University of Ioannina; Πανεπιστήμιο Ιωαννίνων; 2012. [cited 2021 Mar 09]. Available from: http://hdl.handle.net/10442/hedi/28460.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Φωτόπουλος �. Σηπτική αρθρίτις γόνατος: διάγνωση και αντιμετώπιση: κλινική σημασία των ζελατινασών, MMP-2 και MMP-9 στη σηπτική αρθρίτιδα του γόνατος. [Thesis]. University of Ioannina; Πανεπιστήμιο Ιωαννίνων; 2012. Available from: http://hdl.handle.net/10442/hedi/28460

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

20. Μαστοράκη, Σωτηρία. Ο προστατευτικός ρόλος της συμβαστατίνης στη δημιουργία ή επέκταση ανευρυσματικής διάτασης κοιλιακής αορτής.

Degree: 2012, National and Kapodistrian University of Athens; Εθνικό και Καποδιστριακό Πανεπιστήμιο Αθηνών (ΕΚΠΑ)

URL: http://hdl.handle.net/10442/hedi/29582

►

Introduction: Abdominal aortic aneurysm (AAA) is a common and lethal disorder. AAAs are associated with atherosclerosis, chronic inflammation and tissue destruction. The aim of this… (more)

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Introduction: Abdominal aortic aneurysm (AAA) is a common and lethal disorder. AAAs are associated with atherosclerosis, chronic inflammation and tissue destruction. The aim of this study is to determine whether treatment with simvastatin can influence the development of experimental aortic aneurysms in a rabbit model.Materials and methods: A total of 76 rabbits were randomized in four groups: in group I (n=12) where the abdominal aortas were exposed to 0,9 NaCl and in group II (n=24), group III (n=24) and group IV (n=18) where the aortas were exposed to CaCl2 0.5 mol/L for 15 minutes after laparotomy. Group III received 2mg/kg simvastatin daily starting 7 days prior to laparotomy and in group IV the daily treatment with simvastatin started 7 days after laparotomy Animals were sacrificed at intervals of first, second, third and fourth week to obtain measurements of aortic diameter and histological examination. Moreover, immunohistochemistry was used in order to examine the relative distribution of MMP-2, MMP-9 and TIMP-1 within the aortic aneurysms.Results: The increase of aortic diameter in animals of group I was ranging from 4.6% to 7.6%, in group II from 1.2±0.27mm (41%) to 2.33±0.26 mm (85%) (p<0.001 in all cases), in group III from 9-18% (group II vs group III, p<0.001 in all cases) and in group IV from 1.22±0.17mm ( 36%) to 1.25±0.17mm (38%). Moreover, aortic specimens of group II presented a statistically significant increase in MMP-2 and MMP-9 immunoexpression compared to other groups (I, III, IV) (p<0.05 for all comparisons), with the exception of animals of group IV at the end of second week, but not statistically significant increase in TIMP-1 immunoreactivity (p>0.05 for all comparisons. Conclusions: Simvastatin may prove clinically significant in suppressing the development and expansion of AAAs and thereby, in reducing the risk of rupture and the need for surgery.

Εισαγωγή :Το ανεύρυσμα κοιλιακής αορτής (ΑΚΑ) αποτελεί μια συχνή και θανατηφόρο νόσο.Τα ανευρύσματα συνοδεύονται από αθηροσκλήρυνση, χρόνια φλεγμωνή και ιστική αποδόμηση του τοιχώματος του αγγείου.Σκοπός της παρούσας διατριβής είναι να καθοριστεί εάν η θεραπεία με σιμβαστατίνη μπορεί να επηρεάσει την ανάπτυξη αορτικού ανευρύσματος σε πειραματικό μοντέλο με κόνικλους.Υλικό και Μέθοδος: Εβδομήντα οκτώ (78) κουνέλια κατανεμήθηκαν με τυχαίο τρόπο σε τέσσερεις ομάδες: στην ομάδα I (n=12) στα οποία η κοιλιακή αορτή εκπλύθηκε με 0,9 NaCl και στην ομάδα II (n=24), ομάδα III (n=24) και ομάδα IV (n=18) στις οποίες οι αορτές εκτέθηκαν σε CaCl2 0.5 mol/L για 15 λεπτά μετά την λαπαροτομία. Τα πειραματόζωα της ομάδας III έλαβαν per os 2mg/kg σιμβαστατίνη καθημερινά ξεκινώντας 7 μέρες πριν τη λαπαροτομία ενώ στα πειραματόζωα της ομάδας IV η καθημερινή θεραπεία με σιμβαστατίνη ξεκίνησε 7 μέρες μετά τη λαπαροτομία. Τα πειραματόζωα ευθανατώθηκαν σε χρονικά διαστήματα της πρώτης ,δεύτερης ,τρίτης και τέταρτης εβδομάδας ώστε να υποβληθούν σε μέτρηση της αορτικής διαμέτρου και ιστολογική εξέταση αυτής.Επιπρόσθετα ανοσοιστοχημεία χρησιμοποιήθηκε ώστε να…

Subjects/Keywords: Ανεύρυσμα κοιλιακής αορτής; Συμβαστατίνη; Abdominal aortic aneurysm; Simvastatin; MMP-2; MMP-9; TIMP-1

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Μαστοράκη, . �. (2012). Ο προστατευτικός ρόλος της συμβαστατίνης στη δημιουργία ή επέκταση ανευρυσματικής διάτασης κοιλιακής αορτής. (Thesis). National and Kapodistrian University of Athens; Εθνικό και Καποδιστριακό Πανεπιστήμιο Αθηνών (ΕΚΠΑ). Retrieved from http://hdl.handle.net/10442/hedi/29582

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Μαστοράκη, Σωτηρία. “Ο προστατευτικός ρόλος της συμβαστατίνης στη δημιουργία ή επέκταση ανευρυσματικής διάτασης κοιλιακής αορτής.” 2012. Thesis, National and Kapodistrian University of Athens; Εθνικό και Καποδιστριακό Πανεπιστήμιο Αθηνών (ΕΚΠΑ). Accessed March 09, 2021. http://hdl.handle.net/10442/hedi/29582.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Μαστοράκη, Σωτηρία. “Ο προστατευτικός ρόλος της συμβαστατίνης στη δημιουργία ή επέκταση ανευρυσματικής διάτασης κοιλιακής αορτής.” 2012. Web. 09 Mar 2021.

Vancouver:

Μαστοράκη �. Ο προστατευτικός ρόλος της συμβαστατίνης στη δημιουργία ή επέκταση ανευρυσματικής διάτασης κοιλιακής αορτής. [Internet] [Thesis]. National and Kapodistrian University of Athens; Εθνικό και Καποδιστριακό Πανεπιστήμιο Αθηνών (ΕΚΠΑ); 2012. [cited 2021 Mar 09]. Available from: http://hdl.handle.net/10442/hedi/29582.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Μαστοράκη �. Ο προστατευτικός ρόλος της συμβαστατίνης στη δημιουργία ή επέκταση ανευρυσματικής διάτασης κοιλιακής αορτής. [Thesis]. National and Kapodistrian University of Athens; Εθνικό και Καποδιστριακό Πανεπιστήμιο Αθηνών (ΕΚΠΑ); 2012. Available from: http://hdl.handle.net/10442/hedi/29582

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

21. Shamseddin, Aly. Matrix metalloproteinases as potential target in the treatment of vascular dysfunctions : Métalloprotéinases matricielles comme cibles thérapeutiques potentielles dans le traitement des dysfonctionnements vasculaires.

Degree: Docteur es, Immunologie, 2016, Aix-Marseille; Université Laval (Québec, Canada). Faculté de médecine

URL: http://www.theses.fr/2016AIXM4113

► L’endothélium préserve l'homéostasie vasculaire et tissulaire qui contrôle plusieurs processus physiologiques dans les corps humain. Lors de certaines pathologies, l'intégrité vasculaire peut être perturbée par… (more)

▼ L’endothélium préserve l'homéostasie vasculaire et tissulaire qui contrôle plusieurs processus physiologiques dans les corps humain. Lors de certaines pathologies, l'intégrité vasculaire peut être perturbée par une diversité de médiateurs de perméabilité qui interrompent la fonction barrière de cet épithélium, et provoquer des lésions tissulaires au cours de la progression de la maladie. Par conséquent, l'intégrité de la barrière endothéliale est essentielle pour l'homéostasie vasculaire. Dans le cas de la fièvre hémorragique due à la dengue, de nombreuses voies de signalisation induisent une perméabilité vasculaire qui résulte d'une rupture de la barrière hémato-encéphalique qui, dans certains cas, peuvent permettre la pénétration virale dans le système nerveux central (SNC). La plupart de ces voies de signalisation (y compris le TNF-alpha) sur-activent les enzymes appelées métalloprotéinases matricielles (MMP), qui induisent la dégradation de la matrice extracellulaire (MEC) et des protéines intercellulaires régissant la perméabilité vasculaire. Parmi ces enzymes, les MMP gélatinolytiques (MMP-9 et MMP-2) sont considérées comme la classe la plus importante de MMP puisqu'elles sont capables de dégrader le collagène de type IV, constituant principal de la MEC, et les jonctions cellulaires PECAM- 1 et VE-cadhérine. Ainsi, dans cette étude, nous avons développé deux approches pour inhiber l'activité de MMP-9 et protéger la perméabilité vasculaire in vitro. Ces approches incluent (i) des antagonistes de MMP-9 conçus in silico, tels que le composée HA048 (ii) des composés lipophénoliques synthétiques dérivés du resvératrol. Ce dernier existe naturellement dans les tiges de raisin, les arachides et plusieurs autres plantes et qui est capable d'inhiber l'expression et l'activité de la MMP-9. Bien que le resvératrol ait plusieurs activités biologiques testées in vitro, son utilisation dans des modèles animaux est limitée en raison de sa faible biodisponibilité, de son métabolisme rapide et de son élimination. Par conséquent, cette étude a trois objectifs principaux. Premièrement, de trouver un nouveau composé capable d'inhiber l'activité de MMP-9 in vitro. Deuxièmement, de vérifier s’il peut protéger l'intégrité de la couche endothéliale dans les HUVEC. Enfin, de passer à des études précliniques sur le modèle de souris dengue afin de vérifier son activité, sa toxicité et sa biodisponibilité. Outre le resvératrol et le SB-3CT (inhibiteur commercial de la MMP-9), nous avons constaté que le resvératrol-acide linoléique oméga 6 (RES-LA), le resvératrol-docosanoïque (RES-C22) et le HA048 ont montré la plus forte activité anti-MMP-9 parmi d'autres composés. Cependant, RES-LA a été préférentiellement choisi par rapport à RES-C22 en raison de sa meilleure solubilité. Par conséquent, nous avons utilisé un nouveau concept de formulation de ces composés en les solubilisant dans un solvant compatible et moins toxique pour des expériences in vivo, un solvant eutectique profond naturel (NADES), composé de 1,2-propanediol:ChCl: eau… Advisors/Committee Members: Veas, Francisco (thesis director).

Subjects/Keywords: Mmp-9; Resveratrol; Nades; Dengue; Perméabilité endothéliale; Fuite vasculaire; Mmp-9; Resveratrol; Nades; Dengue; Endothelial permeability; Vascular leakage; 571

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Shamseddin, A. (2016). Matrix metalloproteinases as potential target in the treatment of vascular dysfunctions : Métalloprotéinases matricielles comme cibles thérapeutiques potentielles dans le traitement des dysfonctionnements vasculaires. (Doctoral Dissertation). Aix-Marseille; Université Laval (Québec, Canada). Faculté de médecine. Retrieved from http://www.theses.fr/2016AIXM4113

Chicago Manual of Style (16^th Edition):

Shamseddin, Aly. “Matrix metalloproteinases as potential target in the treatment of vascular dysfunctions : Métalloprotéinases matricielles comme cibles thérapeutiques potentielles dans le traitement des dysfonctionnements vasculaires.” 2016. Doctoral Dissertation, Aix-Marseille; Université Laval (Québec, Canada). Faculté de médecine. Accessed March 09, 2021. http://www.theses.fr/2016AIXM4113.

MLA Handbook (7^th Edition):

Shamseddin, Aly. “Matrix metalloproteinases as potential target in the treatment of vascular dysfunctions : Métalloprotéinases matricielles comme cibles thérapeutiques potentielles dans le traitement des dysfonctionnements vasculaires.” 2016. Web. 09 Mar 2021.

Vancouver:

Shamseddin A. Matrix metalloproteinases as potential target in the treatment of vascular dysfunctions : Métalloprotéinases matricielles comme cibles thérapeutiques potentielles dans le traitement des dysfonctionnements vasculaires. [Internet] [Doctoral dissertation]. Aix-Marseille; Université Laval (Québec, Canada). Faculté de médecine; 2016. [cited 2021 Mar 09]. Available from: http://www.theses.fr/2016AIXM4113.

Council of Science Editors:

Shamseddin A. Matrix metalloproteinases as potential target in the treatment of vascular dysfunctions : Métalloprotéinases matricielles comme cibles thérapeutiques potentielles dans le traitement des dysfonctionnements vasculaires. [Doctoral Dissertation]. Aix-Marseille; Université Laval (Québec, Canada). Faculté de médecine; 2016. Available from: http://www.theses.fr/2016AIXM4113

22. Montmasson, Marine. Implication du syndécan-1 dans la migration des kératinocytes : Syndecan-1 involvement in keratinocyte migration.

Degree: Docteur es, Biologie cellulaire et moléculaire, 2018, Lyon

URL: http://www.theses.fr/2018LYSE1330

► Au cours de la réparation cutanée, l’étape de réépithélialisation est essentielle car son objectif est de restaurer la fonction barrière de la peau. Elle consiste… (more)

▼ Au cours de la réparation cutanée, l’étape de réépithélialisation est essentielle car son objectif est de restaurer la fonction barrière de la peau. Elle consiste en une série d’étapes coordonnées où les kératinocytes migrent, prolifèrent et se différencient jusqu’à restauration complète de l’épiderme. Régulée de façon simultanée au niveau intracellulaire mais également extracellulaire, elle dépend de la production de facteurs de croissance, de métalloprotéases matricielles (MMPs) et de protéines de la matrice extracellulaire sur lesquelles les kératinocytes adhèrent et migrent par l’intermédiaire de récepteurs de la famille des intégrines ou des syndécans. Parmi les ligands matriciels, la laminine 332 (LN332), qui est connue comme étant la protéine d’adhésion majeure des kératinocytes de l’épiderme, s’avère être également impliquée au cours de la réépithélialisation et jouer un rôle important dans les processus d’adhésion et de migration des kératinocytes, notamment par le biais de son domaine globulaire LG4/5 localisé à l’extrémité C-term de sa chaine 3. De récentes études ont montré que ce domaine LG4/5 induit la migration des kératinocytes normaux humains (NHK), impliquant des MMPs pro-migratoires MMP-1 et MMP-9. Puisque les domaines LG4/5 ont été montrés comme participant à la dynamique du cytosquelette et au mouvement cellulaire par le biais des récepteurs syndécan-1 et -4, mon laboratoire d’accueil a décidé d’étudier l’implication du récepteur syndécan-1 dans ce processus d’expression de la MMP-9. Les analyses PCR et les résultats de zymographie obtenus ont révélé que le syndécan-1 joue un rôle dans l’expression et l’activation de la MMP-9 induite par le domaine LG4/5. De plus, la déplétion de l’expression du syndécan-1 dans les NHK a confirmé ces résultats. De précédents résultats de mon laboratoire d’accueil ont montré que le domaine LG4/5 induit la formation de filopodes médiée par le syndécan-1 au niveau du front de migration des kératinocytes. De ce fait, nous avons effectué des zymographies de gélatine in situ chez des NHK en migration afin d’observer la localisation de la MMP-9 et de savoir si cette dernière était trouvée au niveau de ces structures d’adhésion protrusives. Nous avons observé des zones de digestion de gélatine sous les NHK ressemblant à des points de contact d’adhésion. Leur nombre est augmenté chez des NHK traités avec le domaine LG4/5 de la LN332. L’utilisation d’inhibiteurs spécifiques des gélatinases et de la MMP-9 ont révélé que cette dernière est responsable de la formation de ces zones de digestion de gélatine. Parce que ces structures évoquent des podosomes, nous avons décidé de révéler leurs constituants majoritaires, à savoir la cortactine, la vinculine, l’-actinine, VASP, WASP ou encore Arp2/3. Dans le même temps nous avons également révélé le syndécan-1 afin de voir si ce dernier était présent dans ces structures. Nos résultats ont montré que tous les marqueurs des podosomes étaient localisés soit sous la forme d’un point à l’intérieur des zones de digestion pour les… Advisors/Committee Members: Rousselle, Patricia (thesis director).

Subjects/Keywords: MMP-9; Syndécan-1; Kératinocyte; Laminine 332; Podosome; MMP-9; Syndecan-1; Keratinocyte; Laminin 332; Podosome; 610

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Montmasson, M. (2018). Implication du syndécan-1 dans la migration des kératinocytes : Syndecan-1 involvement in keratinocyte migration. (Doctoral Dissertation). Lyon. Retrieved from http://www.theses.fr/2018LYSE1330

Chicago Manual of Style (16^th Edition):

Montmasson, Marine. “Implication du syndécan-1 dans la migration des kératinocytes : Syndecan-1 involvement in keratinocyte migration.” 2018. Doctoral Dissertation, Lyon. Accessed March 09, 2021. http://www.theses.fr/2018LYSE1330.

MLA Handbook (7^th Edition):

Montmasson, Marine. “Implication du syndécan-1 dans la migration des kératinocytes : Syndecan-1 involvement in keratinocyte migration.” 2018. Web. 09 Mar 2021.

Vancouver:

Montmasson M. Implication du syndécan-1 dans la migration des kératinocytes : Syndecan-1 involvement in keratinocyte migration. [Internet] [Doctoral dissertation]. Lyon; 2018. [cited 2021 Mar 09]. Available from: http://www.theses.fr/2018LYSE1330.

Council of Science Editors:

Montmasson M. Implication du syndécan-1 dans la migration des kératinocytes : Syndecan-1 involvement in keratinocyte migration. [Doctoral Dissertation]. Lyon; 2018. Available from: http://www.theses.fr/2018LYSE1330

Tampere University

23. Kalela, Anne. Factors affecting serum matrix metalloproteinase-9 with special reference to atherosclerosis .

Degree: Lääketieteen laitos - Medical School, 2002, Tampere University

URL: https://trepo.tuni.fi/handle/10024/67239

► Valtimonkovettumatauti eli ateroskleroosi on hitaasti etenevä verisuonten seinämiä jäykistävä ja ahtauttava tauti. Sen yleisimmät kliiniset ilmenemismuodot ovat sepelvaltimotauti ja aivoverenkierron sairaudet. Valtimon seinämän tulehdusta pidetään… (more)

Subjects/Keywords: ateroskleroosi ; mmp-9 ; tulehdus ; atherosclerosis ; mmp-9 ; inflammation

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APA (6^th Edition):

Kalela, A. (2002). Factors affecting serum matrix metalloproteinase-9 with special reference to atherosclerosis . (Doctoral Dissertation). Tampere University. Retrieved from https://trepo.tuni.fi/handle/10024/67239

Chicago Manual of Style (16^th Edition):

Kalela, Anne. “Factors affecting serum matrix metalloproteinase-9 with special reference to atherosclerosis .” 2002. Doctoral Dissertation, Tampere University. Accessed March 09, 2021. https://trepo.tuni.fi/handle/10024/67239.

MLA Handbook (7^th Edition):

Kalela, Anne. “Factors affecting serum matrix metalloproteinase-9 with special reference to atherosclerosis .” 2002. Web. 09 Mar 2021.

Vancouver:

Kalela A. Factors affecting serum matrix metalloproteinase-9 with special reference to atherosclerosis . [Internet] [Doctoral dissertation]. Tampere University; 2002. [cited 2021 Mar 09]. Available from: https://trepo.tuni.fi/handle/10024/67239.

Council of Science Editors:

Kalela A. Factors affecting serum matrix metalloproteinase-9 with special reference to atherosclerosis . [Doctoral Dissertation]. Tampere University; 2002. Available from: https://trepo.tuni.fi/handle/10024/67239

24. Kazakou, Aikaterini. Η κλινική αξία της οστεοποντίνης στον καρκίνο του πνεύμονα.

Degree: 2016, University of Thessaly (UTH); Πανεπιστήμιο Θεσσαλίας

URL: http://hdl.handle.net/10442/hedi/39378

►

INTRODUCTION: Osteopontin (OPN) is a multifunctional glycoprotein found in various tissues. It plays an important role in physiological and pathological processes, including cancer biology.PURPOSE: The… (more)

▼

INTRODUCTION: Osteopontin (OPN) is a multifunctional glycoprotein found in various tissues. It plays an important role in physiological and pathological processes, including cancer biology.PURPOSE: The aim of this study was a) to evaluate OPN serum levels in lung cancer patients in comparison with samples from healthy volunteers, b) to correlate pre-treatment OPN serum levels with clinicopathological parameters, response to therapy and overall survival and c) to seek any possible association of OPN levels with circulating vascular endothelial growth factor (VEGF) and matrix metalloproteinase-9 (MMP-9).MATERIALS AND METHODS: In the present study, 90 patients with primary lung cancer were enrolled - mean age 66.22±9.09 years (70 with Non Small Cell Lung Cancer and 20 with Small Cell Lung Cancer) - and 40 healthy subjects were used as a control group. The serum levels of OPN, VEGF and MMP-9 were measured by ELISA (Enzyme-Linked Immunosorbent Assay).RESULTS: OPN serum levels were elevated in lung cancer patients compared to healthy subjects (52.10 ng/ml vs. 22.98 ng/ml, p<0.0001). In addition, smoker patients showed significantly higher levels of OPN and MMP-9 than non-smokers and ex-smokers (p=0.012 and p=0.008, respectively). Also, patients with worse performance status exhibited higher levels of OPN, VEGF and MMP-9 (p=0.0036, p<0.0001 and p=0.0004, respectively). Those who had significant weight loss, displayed higher levels of OPN (p=0.0003). Furthermore, patients without evidence of metastatic disease in the lymph nodes, had lower levels of VEGF and MMP-9 (p=0.0101 and p=0.0144, respectively). There was no association of OPN, VEGF and MMP-9 serum levels with response to chemotherapy. Patients with OPN circulating levels above the median value had worse overall survival (39 weeks vs. 64.6 weeks, p=0.0018, log-rank test). In addition, logistic regression analysis demonstrated that OPN serum levels trended towards being an independent prognostic factor for overall survival (p=0.0656, 95% CI 0.9723-2.6563). Similarly worse overall survival, was found for the individuals with values of VEGF above the median value (43.30 weeks vs. 62.90 weeks, p=0.0065). Finally, OPN serum levels were positively correlated with both VEGF and MMP-9 levels (p=0.0008, Spearman r=0.36, and p<0.0001, Spearman r=0.42, respectively).CONCLUSION: In lung cancer patients, OPN might serve as a prognostic biomarker. Furthermore, the positive correlation between OPN and both VEGF and MMP-9 could implicate new insights in tumour angiogenesis.

ΕΙΣΑΓΩΓΗ: Η οστεοποντίνη (OPN) είναι μια πολυλειτουργική γλυκοπρωτεΐνη, που ανευρίσκεται σε διάφορους ιστούς και διαδραματίζει σημαντικό ρόλο σε φυσιολογικές και παθολογικές διεργασίες, συμπεριλαμβανομένης της βιολογίας του καρκίνου. ΣΚΟΠΟΣ: Ο σκοπός της μελέτης ήταν α) ο προσδιορισμός των επιπέδων της OPN στον ορό αίματος ασθενών με καρκίνο πνεύμονα και σύγκριση με δείγματα υγιών εθελοντών, β) η συσχέτιση των, προ θεραπείας, επιπέδων OPN, αγγειακού ενδοθηλιακού αυξητικού παράγοντα (VEGF) και…

Subjects/Keywords: Οστεοποντίνη (OPN); VEGF (Aγγειογενετικός ενδοθηλιακός αναπτυξιακός παράγοντας ); Μεταλλοπρωτεάση - 9 ( MMP-9 ); Προγνωστικοί δείκτες; Καρκίνος πνεύμονα; Osteopontin (OPN); Vascular endothelial growth factor (VEGF); Metalloprotease - 9 (MMP-9); Prognostic biomarkers; Lung cancer

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Kazakou, A. (2016). Η κλινική αξία της οστεοποντίνης στον καρκίνο του πνεύμονα. (Thesis). University of Thessaly (UTH); Πανεπιστήμιο Θεσσαλίας. Retrieved from http://hdl.handle.net/10442/hedi/39378

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Kazakou, Aikaterini. “Η κλινική αξία της οστεοποντίνης στον καρκίνο του πνεύμονα.” 2016. Thesis, University of Thessaly (UTH); Πανεπιστήμιο Θεσσαλίας. Accessed March 09, 2021. http://hdl.handle.net/10442/hedi/39378.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Kazakou, Aikaterini. “Η κλινική αξία της οστεοποντίνης στον καρκίνο του πνεύμονα.” 2016. Web. 09 Mar 2021.

Vancouver:

Kazakou A. Η κλινική αξία της οστεοποντίνης στον καρκίνο του πνεύμονα. [Internet] [Thesis]. University of Thessaly (UTH); Πανεπιστήμιο Θεσσαλίας; 2016. [cited 2021 Mar 09]. Available from: http://hdl.handle.net/10442/hedi/39378.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Kazakou A. Η κλινική αξία της οστεοποντίνης στον καρκίνο του πνεύμονα. [Thesis]. University of Thessaly (UTH); Πανεπιστήμιο Θεσσαλίας; 2016. Available from: http://hdl.handle.net/10442/hedi/39378

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

25. Silva, Nicholas. Inflammation and Matrix Metalloproteinase 9 (Mmp-9) Function in the Zebrafish Retina.

Degree: PhD, Neuroscience, 2019, University of Michigan

URL: http://hdl.handle.net/2027.42/149832

► Inflammation in the central nervous system (CNS) activates a complex network of signaling molecules in dying neurons, surviving neurons, and glia. In contrast to mammals,… (more)

Subjects/Keywords: Regeneration; Retina; Zebrafish; Inflammation; Matrix Metalloproteinase 9 (Mmp-9); Science (General); Science

10 more images…

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APA (6^th Edition):

Silva, N. (2019). Inflammation and Matrix Metalloproteinase 9 (Mmp-9) Function in the Zebrafish Retina. (Doctoral Dissertation). University of Michigan. Retrieved from http://hdl.handle.net/2027.42/149832

Chicago Manual of Style (16^th Edition):

Silva, Nicholas. “Inflammation and Matrix Metalloproteinase 9 (Mmp-9) Function in the Zebrafish Retina.” 2019. Doctoral Dissertation, University of Michigan. Accessed March 09, 2021. http://hdl.handle.net/2027.42/149832.

MLA Handbook (7^th Edition):

Silva, Nicholas. “Inflammation and Matrix Metalloproteinase 9 (Mmp-9) Function in the Zebrafish Retina.” 2019. Web. 09 Mar 2021.

Vancouver:

Silva N. Inflammation and Matrix Metalloproteinase 9 (Mmp-9) Function in the Zebrafish Retina. [Internet] [Doctoral dissertation]. University of Michigan; 2019. [cited 2021 Mar 09]. Available from: http://hdl.handle.net/2027.42/149832.

Council of Science Editors:

Silva N. Inflammation and Matrix Metalloproteinase 9 (Mmp-9) Function in the Zebrafish Retina. [Doctoral Dissertation]. University of Michigan; 2019. Available from: http://hdl.handle.net/2027.42/149832

26. Paltatzidou, Kiriaki. Συγκριτική μελέτη θεραπείας χηλοειδών με 5 - φθοριουρακίλη, κρυοχειρουργικής και συνδυασμών αυτών με τη χρήση ανοσοϊστοχημικών τεχνικών.

Degree: 2018, National and Kapodistrian University of Athens; Εθνικό και Καποδιστριακό Πανεπιστήμιο Αθηνών (ΕΚΠΑ)

URL: http://hdl.handle.net/10442/hedi/43284

► SummaryObjectivesKeloids are a type of benign proliferative fibromas that form following trauma. Metalloproteinase-9 (MMP-9), produced in multinuclear giant cells,1 is considered a subclass of the… (more)

▼ SummaryObjectivesKeloids are a type of benign proliferative fibromas that form following trauma. Metalloproteinase-9 (MMP-9), produced in multinuclear giant cells,1 is considered a subclass of the MMPs (a family of zinc-dependent endopeptidases), and is involved in processes that occur during cutaneous wound healing such as, inflammation matrix remodelling and epithelization. The activity of MMP-9 has been shown to be critical for several of these processes in the content of pathological conditions, such as hypertrophic scars and keloids. In addition, a pyrimidine analogue with antimetabolite activity, 5-fluorouracil,when given intralesionally, is an alternative therapy for keloids.Intralesional cryotherapy is a technique for treatment of keloids which can have uncomfortable side-effects like, pain, oedema, infections, hypoesthesia, milium cyst formation, necrosis and persistent hypopigmentation. In this study we investigated the effect of three different keloid treatments in immunoreactivity of MMP-9 in multinuclear giant cells in skin keloids. Material and methodForty five patients were enrolled in this study. Pregnant women, women planning pregnancy in the near future, patients with chronic renal failure or abnormal liver function tests or full blood count were excluded from the study. All patients were treatment naıve. The study consisted of three treatment arms, as described below:•5-Fluorouracil (5-FU) arm: Fifteen patients were treated once weekly with intralesional 5-FU 50 mg/cc. The delivered dose was adjusted according to the extent of the lesions, and did not exceed 100 mg per session (2 cc). The solution was injected using a 27-gauge needle until slight blanching was clinically visible. Only the indurated parts of the keloids were treated by multiple injections, separated by 1 cm approximately.•Cryotherapy arms: Fifteen patients were treated once monthly with intralesional cryotherapy. •Combination arm: Fifteen patients were treated once monthly with intralesional 5-FU plus cryotherapy.Lesional skin biopsies were taken from patients with skin keloids, before introduction of any treatment and after treatments, to assess the MMP-9 immunohistochemical result.Immunochemistry stain of MMP-9 was carried out using monoclonal antibody DACO (anti-MMP-9 from DAKOAO150) as described in Marzano et al., 2010.Two independent blind observers evaluated the serial sections. Immunoreactivity was scored according to the number of immunoreactive cells per field (9200), as follows: 0 = no immunoreactive cells; 1 = 1–5 cells; 2 = 6–20 cells; 3 = ≥20 cells. A P-value of <0.05 was considered to indicate a statistically significant difference. ResultsMMP-9 was expressed strongly at the multinuclear giant cells of keloid biopsies. The score according to the number of immunoreactive multinuclear giant cells between pre and post treatment was significantly decreased in the three therapies (P < 0.05).The specific role of MMP-9 in normal healing is not known. It has been reported that MMP-9 is present during normal healing for the…

Subjects/Keywords: Χηλοειδή; 5-φθοριουρακίλη; Μεταλλοπρωτεϊνάση-9; Κρυοθεραπεία; Keloids; MMP-9; 5-FU; Cryotherapy; 5-Fluoruracil

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Paltatzidou, K. (2018). Συγκριτική μελέτη θεραπείας χηλοειδών με 5 - φθοριουρακίλη, κρυοχειρουργικής και συνδυασμών αυτών με τη χρήση ανοσοϊστοχημικών τεχνικών. (Thesis). National and Kapodistrian University of Athens; Εθνικό και Καποδιστριακό Πανεπιστήμιο Αθηνών (ΕΚΠΑ). Retrieved from http://hdl.handle.net/10442/hedi/43284

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Paltatzidou, Kiriaki. “Συγκριτική μελέτη θεραπείας χηλοειδών με 5 - φθοριουρακίλη, κρυοχειρουργικής και συνδυασμών αυτών με τη χρήση ανοσοϊστοχημικών τεχνικών.” 2018. Thesis, National and Kapodistrian University of Athens; Εθνικό και Καποδιστριακό Πανεπιστήμιο Αθηνών (ΕΚΠΑ). Accessed March 09, 2021. http://hdl.handle.net/10442/hedi/43284.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Paltatzidou, Kiriaki. “Συγκριτική μελέτη θεραπείας χηλοειδών με 5 - φθοριουρακίλη, κρυοχειρουργικής και συνδυασμών αυτών με τη χρήση ανοσοϊστοχημικών τεχνικών.” 2018. Web. 09 Mar 2021.

Vancouver:

Paltatzidou K. Συγκριτική μελέτη θεραπείας χηλοειδών με 5 - φθοριουρακίλη, κρυοχειρουργικής και συνδυασμών αυτών με τη χρήση ανοσοϊστοχημικών τεχνικών. [Internet] [Thesis]. National and Kapodistrian University of Athens; Εθνικό και Καποδιστριακό Πανεπιστήμιο Αθηνών (ΕΚΠΑ); 2018. [cited 2021 Mar 09]. Available from: http://hdl.handle.net/10442/hedi/43284.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Paltatzidou K. Συγκριτική μελέτη θεραπείας χηλοειδών με 5 - φθοριουρακίλη, κρυοχειρουργικής και συνδυασμών αυτών με τη χρήση ανοσοϊστοχημικών τεχνικών. [Thesis]. National and Kapodistrian University of Athens; Εθνικό και Καποδιστριακό Πανεπιστήμιο Αθηνών (ΕΚΠΑ); 2018. Available from: http://hdl.handle.net/10442/hedi/43284

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

NSYSU

27. Liu, Wen-Hsin. The molecular mechanisms involve in proliferation and metastasis of human leukemic U937 and K562 cells.

Degree: PhD, Institute of Biomedical Sciences, 2011, NSYSU

URL: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0616111-104640

► Leukemia is a hematological neoplasm with abnormal genetic mutation or chromosomal translocation in the myeloblast or lymphoblast, and characterized by accumulation of immature cells and… (more)

▼ Leukemia is a hematological neoplasm with abnormal genetic mutation or chromosomal translocation in the myeloblast or lymphoblast, and characterized by accumulation of immature cells and malfunction of lymphocytes and myeloid-derived cells. The prognosis of treatment depends on genetic mutation, chromosomal aberration, disease progression and age of patients. Currently, bone marrow transplantation is a useful therapeutic strategy, but the success in therapy is limited by the bone marrow of donors and life-threatening events such as immune repulsion. Although chemotherapy improves leukemia treatment, long-term chemotherapy usually leads to the production of drug-resistant cancer cells. Thus, the development of new modality in overcoming drug-resistant should be beneficial for in leukemia therapy. In this thesis, Naja nigricollis toxin Î³, piceatannol, caffeine, and Bungarus multicinctus protease inhibitor-like protein 1 (PILP-1) are employed to investigate the molecular mechanisms in regulating apoptosis and invasion of leukemic cell lines K562 and U937. Hopefully, the signaling pathways elicited by these treatments may be aid in identifying new targets in treating leukemia. Toxin Î³ inducing cell death is found to evoke p38 MAPK-mediated Bcl-2 down-regulation, which facilitates mitochondria dysfunction, ROS generation and cytiochrome c release. Finally, activation of caspases leads to apoptotic death of toxin Î³-treated cells. Piceatannol elicits Ca2+/p38Î± MAPK- mediated c-Jun and ATF-2 phosphorylation, leading to up-regulation of Fas/FasL protein expression and autocrine Fas-mediated death pathway activation. Caffeine treatment down-regulates MMP-2/-9 down-regulation via Ca2+/ROS-mediated inactivation of ERK/c-Fos and activation of p38 MAPK/c-Jun pathway. Consequently, caffeine treatment suppresses invasion of leukemia cells. PILP-1-induced ADAM17 down-regulation suppresses Lyn-mediated Akt phosphorylation, resulting in death of PILP-1-treated leukemia cells. Taken together, the results of the present study elucidate the signaling pathways responsible for apoptosis and invasion of leukemia cells. Moreover, these findings might suggest new targets in developing therapeutic strategy in treating leukemia. Advisors/Committee Members: Long-Sen Chang (committee member), Chun-Chang Chang (chair), Shinne-Ren Lin (chair), Kuang-Hung Cheng (chair), Chi-Huei Wang (chair).

Subjects/Keywords: Leukemia; MAPKs; Bcl-2; Fas/FasL; MMP-2/-9; ADAM17

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APA (6^th Edition):

Liu, W. (2011). The molecular mechanisms involve in proliferation and metastasis of human leukemic U937 and K562 cells. (Doctoral Dissertation). NSYSU. Retrieved from http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0616111-104640

Chicago Manual of Style (16^th Edition):

Liu, Wen-Hsin. “The molecular mechanisms involve in proliferation and metastasis of human leukemic U937 and K562 cells.” 2011. Doctoral Dissertation, NSYSU. Accessed March 09, 2021. http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0616111-104640.

MLA Handbook (7^th Edition):

Liu, Wen-Hsin. “The molecular mechanisms involve in proliferation and metastasis of human leukemic U937 and K562 cells.” 2011. Web. 09 Mar 2021.

Vancouver:

Liu W. The molecular mechanisms involve in proliferation and metastasis of human leukemic U937 and K562 cells. [Internet] [Doctoral dissertation]. NSYSU; 2011. [cited 2021 Mar 09]. Available from: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0616111-104640.

Council of Science Editors:

Liu W. The molecular mechanisms involve in proliferation and metastasis of human leukemic U937 and K562 cells. [Doctoral Dissertation]. NSYSU; 2011. Available from: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0616111-104640

NSYSU

28. Jhang, Li-Mei. Effect of Gallic Acid on EGF-Induced MMP-9 Expression , Migration and Invasion in Human Breast Cancer MCF-7 Cells.

Degree: Master, Institute of Biomedical Sciences, 2015, NSYSU

URL: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0607115-102253

► Matrix metalloproteinases (MMPs) play a key role in the degradation of extracellular matrix, and up-regulation of MMP expression promotes invasion and migration of cancer cells.… (more)

Subjects/Keywords: breast cancer; Gallic acid; EGF; MMP-9; EGFR

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APA (6^th Edition):

Jhang, L. (2015). Effect of Gallic Acid on EGF-Induced MMP-9 Expression , Migration and Invasion in Human Breast Cancer MCF-7 Cells. (Thesis). NSYSU. Retrieved from http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0607115-102253

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Jhang, Li-Mei. “Effect of Gallic Acid on EGF-Induced MMP-9 Expression , Migration and Invasion in Human Breast Cancer MCF-7 Cells.” 2015. Thesis, NSYSU. Accessed March 09, 2021. http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0607115-102253.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Jhang, Li-Mei. “Effect of Gallic Acid on EGF-Induced MMP-9 Expression , Migration and Invasion in Human Breast Cancer MCF-7 Cells.” 2015. Web. 09 Mar 2021.

Vancouver:

Jhang L. Effect of Gallic Acid on EGF-Induced MMP-9 Expression , Migration and Invasion in Human Breast Cancer MCF-7 Cells. [Internet] [Thesis]. NSYSU; 2015. [cited 2021 Mar 09]. Available from: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0607115-102253.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Jhang L. Effect of Gallic Acid on EGF-Induced MMP-9 Expression , Migration and Invasion in Human Breast Cancer MCF-7 Cells. [Thesis]. NSYSU; 2015. Available from: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0607115-102253

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Dalhousie University

29. Savage, Kimberley. THE DEVELOPMENT OF AN IN VITRO MODEL TO EXAMINE AND MODULATE HEPATIC ISCHEMIA AND REPERFUSION RESPONSES.

Degree: MS, Department of Pathology, 2011, Dalhousie University

URL: http://hdl.handle.net/10222/14249

► Transplantation is the optimal form of therapy for patients with end-stage liver disease; however, the use of organs with hepatic steatosis is often associated with… (more)

Subjects/Keywords: In vitro ischemia reperfusion; Gene therapy; Hepatocytes; MMP-9; HO-1

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Savage, K. (2011). THE DEVELOPMENT OF AN IN VITRO MODEL TO EXAMINE AND MODULATE HEPATIC ISCHEMIA AND REPERFUSION RESPONSES. (Masters Thesis). Dalhousie University. Retrieved from http://hdl.handle.net/10222/14249

Chicago Manual of Style (16^th Edition):

Savage, Kimberley. “THE DEVELOPMENT OF AN IN VITRO MODEL TO EXAMINE AND MODULATE HEPATIC ISCHEMIA AND REPERFUSION RESPONSES.” 2011. Masters Thesis, Dalhousie University. Accessed March 09, 2021. http://hdl.handle.net/10222/14249.

MLA Handbook (7^th Edition):

Savage, Kimberley. “THE DEVELOPMENT OF AN IN VITRO MODEL TO EXAMINE AND MODULATE HEPATIC ISCHEMIA AND REPERFUSION RESPONSES.” 2011. Web. 09 Mar 2021.

Vancouver:

Savage K. THE DEVELOPMENT OF AN IN VITRO MODEL TO EXAMINE AND MODULATE HEPATIC ISCHEMIA AND REPERFUSION RESPONSES. [Internet] [Masters thesis]. Dalhousie University; 2011. [cited 2021 Mar 09]. Available from: http://hdl.handle.net/10222/14249.

Council of Science Editors:

Savage K. THE DEVELOPMENT OF AN IN VITRO MODEL TO EXAMINE AND MODULATE HEPATIC ISCHEMIA AND REPERFUSION RESPONSES. [Masters Thesis]. Dalhousie University; 2011. Available from: http://hdl.handle.net/10222/14249

University of Tasmania

30. Sohal, SS. Epithelial activation in chronic obstructive pulmonary disease (COPD).

Degree: 2010, University of Tasmania

URL: https://eprints.utas.edu.au/10765/34/Sohal_whole%20_thesis.pdf ; Sohal, SS ORCID: 0000-0001-9627-6498 <https://orcid.org/0000-0001-9627-6498> 2010 , 'Epithelial activation in chronic obstructive pulmonary disease (COPD)', PhD thesis, University of Tasmania.

► `Background:` Early on I identified that reticular basement membrane (Rbm) in current smokers with COPD was highly fragmented, with cracks termed “clefts” containing cells. This… (more)

▼ `Background:` Early on I identified that reticular basement membrane (Rbm) in current smokers with COPD was highly fragmented, with cracks termed “clefts” containing cells. This looked like the described hallmark of EMT (epithelial mesenchymal transition). I followed this preliminary observation with a comprehensive cross-sectional study in which I hypothesized that the airway epithelium is activated in smokers, and that this may promote EMT, but that this will be especially active in COPD. As a part of my thesis, I also investigated the expression and activity of the anti-inflammatory enzyme HDAC2 (histone deacetylase 2) which is reported to be reduced in COPD lungs and may account for associated pulmonary inflammation. I hypothesised that the current literature is correct in stating that HDAC2 is down-regulated in COPD airways and also that these reduced HDAC2 levels are normalised by aggressive inhaled corticosteroid (ICS) therapy and smoking cessation in patients with COPD. (Methods) (and) (results:) Endobronchial biopsies (ebb) from current smokers with COPD (COPD-CS) and ex-smokers with COPD (COPD-ES), smokers with normal lung function (NS) and never-smoking controls (NC) stained for markers of EMT, matrix metalloproteinase-9 (MMP-9), fibroblast protein (S100A4), epidermal growth factor receptor (EGFR), vimentin and cytokeratins. To confirm the extent of suppressed HDAC2 ebb were immuno-stained for HDAC2. In a double-blind, randomized, placebo-controlled study, I assessed the effects of ICS on Rbm fragmentation and HDAC2. Compared to NC, there was significant fragmentation of the Rbm in COPD-CS, COPD-ES and NS groups. COPD-CS, NS and COPD-ES demonstrated increases in staining for: basal epithelial S100A4, epithelial EGFR and MMP-9 and S100A4 for cells in Rbm ``clefts`` compared to NC. Dual staining revealed that vimentin (a mesenchymal marker) co-stained with cytokeratin (an epithelial marker). ICS normalised Rbm fragmentation. Compared to NC there was significant increase in HDAC2 positive cells in NS in the lamina propria (LP) but a decrease in COPD-CS. However, this latter abnormality was due to a reduction in total LP cells and not % cell HDAC2 staining. There were significantly more HDAC2 positive cells in COPD-ES compared to COPD-CS, but again due to an increase in total cell numbers. ICS made no difference to HDAC2 staining. `Conclusions:` This is the first description of likely EMT in smoking and COPD. ICS reversed Rbm fragmentation. HDAC2 expression was reduced in smokers but confounded by changes in cellularity. Quitting does seem to have a real effect on upregulating HDAC2, but it is not affected by ICS.

Subjects/Keywords: EMT; COPT; HDAC-2; S100A4; MMP-9; EGFR; airway remodelling

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Sohal, S. (2010). Epithelial activation in chronic obstructive pulmonary disease (COPD). (Thesis). University of Tasmania. Retrieved from https://eprints.utas.edu.au/10765/34/Sohal_whole%20_thesis.pdf ; Sohal, SS ORCID: 0000-0001-9627-6498 <https://orcid.org/0000-0001-9627-6498> 2010 , 'Epithelial activation in chronic obstructive pulmonary disease (COPD)', PhD thesis, University of Tasmania.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Sohal, SS. “Epithelial activation in chronic obstructive pulmonary disease (COPD).” 2010. Thesis, University of Tasmania. Accessed March 09, 2021. https://eprints.utas.edu.au/10765/34/Sohal_whole%20_thesis.pdf ; Sohal, SS ORCID: 0000-0001-9627-6498 <https://orcid.org/0000-0001-9627-6498> 2010 , 'Epithelial activation in chronic obstructive pulmonary disease (COPD)', PhD thesis, University of Tasmania..

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Sohal, SS. “Epithelial activation in chronic obstructive pulmonary disease (COPD).” 2010. Web. 09 Mar 2021.

Vancouver:

Sohal S. Epithelial activation in chronic obstructive pulmonary disease (COPD). [Internet] [Thesis]. University of Tasmania; 2010. [cited 2021 Mar 09]. Available from: https://eprints.utas.edu.au/10765/34/Sohal_whole%20_thesis.pdf ; Sohal, SS ORCID: 0000-0001-9627-6498 <https://orcid.org/0000-0001-9627-6498> 2010 , 'Epithelial activation in chronic obstructive pulmonary disease (COPD)', PhD thesis, University of Tasmania..

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Sohal S. Epithelial activation in chronic obstructive pulmonary disease (COPD). [Thesis]. University of Tasmania; 2010. Available from: https://eprints.utas.edu.au/10765/34/Sohal_whole%20_thesis.pdf ; Sohal, SS ORCID: 0000-0001-9627-6498 <https://orcid.org/0000-0001-9627-6498> 2010 , 'Epithelial activation in chronic obstructive pulmonary disease (COPD)', PhD thesis, University of Tasmania.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

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