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1.
Sales, Kevin Costa.
Fourier transform infrared spectroscopy, a powerful tool to monitor biopharmaceuticals production.
Degree: 2014, RCAAP
URL: http://www.rcaap.pt/detail.jsp?id=oai:repositorio.ucp.pt:10400.14/15655
► A Escherichia coli é o microorganismo mais usado como hospedeiro para a produção de produtos recombinantes, tais como plasmídeos usados para terapia génica e vacinação…
(more)
▼ A Escherichia coli é o microorganismo mais usado como hospedeiro para a produção de produtos recombinantes, tais como plasmídeos usados para terapia génica e vacinação de ADN. Desta forma, torna-se importante compreender as relações metabólicas complexas e a bioprodução de plasmídeo, que ocorre em ambientes de cultura dinâmicos, a fim de controlar e optimizar o desempenho do sistema de expressão recombinante. O objectivo principal deste trabalho consiste em avaliar a potencialidade da espectroscopia FT-IR para monitorizar e caracterizar a produção do plasmídeo pVAX-LacZ em culturas recombinantes de E. coli, nomeadamente para extrair informação relacionada com as variáveis críticas (biomassa, plasmídeo, fontes de carbono e acetato) e informação metabólica da célula hospedeira E. coli. Para tal, culturas de E. coli com diferentes concentrações de glucose e glicerol e diferentes estratégias de cultivo (batch e fed-batch) foram monitorizadas por espectroscopia de infravermelho perto (NIR) e de infravermelho médio (MIR).
Tanto a espectroscopia NIR com a MIR permitiram extrair informação sobre as variáveis críticas do bioprocesso, através da construção de modelos de regressão por mínimos quadrados parciais, que resultaram em elevados coeficientes de regressão e baixos erros de previsão. A abordagem NIR apresenta a vantagem de aquisição em tempo real das variáveis do bioprocesso, já a abordagem MIR permite a leitura simultânea de centenas de amostras de várias culturas ao mesmo tempo através do uso multi-microplacas, sendo muito vantajosa nos casos de micro-bioreactores usados para optimização. Para além disso, como os espectros MIR apresentam mais informação do que os espectros NIR, uma vez que representam os modos de vibração fundamentais das biomoléculas, enquanto que os espectros NIR representam sobreposições e combinações de vibrações, os dados espectrais MIR também permitiram a aquisição de informação bioquímica ao longo das culturas de E. coli a partir da análise das componentes principais (PCA) bem como do estudo das características bioquímicas, tais como as reservas de glicogénio e os níveis de transcrição aparente.
Portanto, a espectroscopia FT-IR apresenta assim características relevantes para a compreensão e monitorização do processo de produção de culturas recombinantes, sendo, de acordo com Quality-by-Design e Process Analytical Technology, muito importante para fins de controlo e optimização.
Escherichia coli is the most used microorganism as host for the production of recombinant products, such as plasmids used for gene therapy and DNA vaccination. Therefore, it is important to understand the complex metabolic relationships and the plasmid bioproduction process occurring in dynamic culture environments, in order to control and optimize the performance of the recombinant expression system. The main goal of this work is to evaluate the potential of Fourier Transform Infrared (FT-IR) spectroscopy to monitor and characterize recombinant E. coli cultures producing the plasmid model pVAX-LacZ, namely to extract…
Advisors/Committee Members: Calado, Cecília Ribeiro da Cruz.
Subjects/Keywords: Escherichia coli; Espectroscopia MIR; Espectroscopia NIR; Caraterização Metabólica; Monitorização de Bioprocessos; MIR Spectroscopy; NIR Spectroscopy; Metabolic Profiling; Bioprocess Monitoring
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APA (6th Edition):
Sales, K. C. (2014). Fourier transform infrared spectroscopy, a powerful tool to monitor biopharmaceuticals production. (Thesis). RCAAP. Retrieved from http://www.rcaap.pt/detail.jsp?id=oai:repositorio.ucp.pt:10400.14/15655
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Sales, Kevin Costa. “Fourier transform infrared spectroscopy, a powerful tool to monitor biopharmaceuticals production.” 2014. Thesis, RCAAP. Accessed January 27, 2021.
http://www.rcaap.pt/detail.jsp?id=oai:repositorio.ucp.pt:10400.14/15655.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Sales, Kevin Costa. “Fourier transform infrared spectroscopy, a powerful tool to monitor biopharmaceuticals production.” 2014. Web. 27 Jan 2021.
Vancouver:
Sales KC. Fourier transform infrared spectroscopy, a powerful tool to monitor biopharmaceuticals production. [Internet] [Thesis]. RCAAP; 2014. [cited 2021 Jan 27].
Available from: http://www.rcaap.pt/detail.jsp?id=oai:repositorio.ucp.pt:10400.14/15655.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Sales KC. Fourier transform infrared spectroscopy, a powerful tool to monitor biopharmaceuticals production. [Thesis]. RCAAP; 2014. Available from: http://www.rcaap.pt/detail.jsp?id=oai:repositorio.ucp.pt:10400.14/15655
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Melbourne
2.
Jangammanaidu Krishnaraj, Saravanan.
Effects of fire on soil organic carbon dynamics in Eucalyptus forests of south-eastern Australia.
Degree: 2014, University of Melbourne
URL: http://hdl.handle.net/11343/40982
► This project investigated the impact of fires (prescribed fire and wild fire) on soil organic carbon dynamics and charcoal formation in southern Australian Eucalyptus forest…
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▼ This project investigated the impact of fires (prescribed fire and wild fire) on soil organic carbon dynamics and charcoal formation in southern Australian Eucalyptus forest soils with two different focuses. The first study aimed to assess the immediate effects of fuel reduction burning on organic carbon in forest floor and mineral soil of Eucalyptus lowland forests in Otway Ranges, south-eastern Australia. The fire burnt the forest floor in a mosaic pattern, a typical feature of management fires. Organic carbon was redistributed from the forest floor to the humus and surface mineral soil horizons in response to fire. With a 38% loss of litter mass, equivalent to a reduction of 3.3 Mg C ha-1 from litter the surface soil organic carbon increased by 4.4g kg-1 in concentration. A chemical oxidation technique was employed to estimate char carbon content of the surface soil before and after fuel reduction burning. The pre-fire soils contained about 11% of total organic carbon as char carbon and burning increased this by 2%. Two weeks after fire the effects on soil organic carbon were restricted to the top 2cm of soil.
The second study aimed to determine the amounts and forms of soil organic carbon in two productive, fire-sensitive ash forests (alpine ash and mountain ash) of southern Australia, under two bushfire regimes. Growing at relatively higher elevation in cooler temperature and higher precipitation, alpine ash showed different soil organic matter decomposition pattern in comparison with mountain ash forests. Part of the even-aged stands in both forests that formed after the wildfire in 1939 was burnt again during 2009 intense fires. Though higher amount of forest floor biomass in mountain ash than alpine ash, the organic carbon densities of 9.6 Mg C ha-1 in alpine ash and 9.3 Mg C ha-1 in mountain ash were observed in 1939 regrowth and the recent fire in 2009 reduced the biomass as well as carbon density in each forest by about 35-55%. In top 30 cm soil, a range of 225-255 Mg C ha-1 in alpine ash and 185-212 Mg C ha-1 in mountain ash forests were recorded. About 15-20% of soil organic carbon was char carbon in these two ash forests as estimated by chemical oxidation. Though there was no difference in isotopic signatures among fire regimes, a little higher 13C depletion in both forest floor and mineral soils of mountain ash than alpine ash forests was observed. The measurable soil carbon pools of varying stability namely particulate organic carbon (POC), humus organic carbon (HOC) and resistant organic carbon (ROC) were quantified by physical fractionation (2000-50μm and ≤50μm size fractions) combined with 13C nuclear magnetic resonance (NMR) spectroscopic analysis. Each of these three organic carbon pools contributed about one-third of the total organic carbon in top 30 cm soil. About 30-70% of ROC was associated with the coarse and fine fractions with an average close to 50% for each. The percentage contribution of ROC to total organic carbon was similar in both ash forests (about 29%). In addition to…
Subjects/Keywords: soil organic carbon; char carbon; prescribed fire; MIR-PLSR; NMR spectroscopy; resistant organic carbon
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APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Jangammanaidu Krishnaraj, S. (2014). Effects of fire on soil organic carbon dynamics in Eucalyptus forests of south-eastern Australia. (Doctoral Dissertation). University of Melbourne. Retrieved from http://hdl.handle.net/11343/40982
Chicago Manual of Style (16th Edition):
Jangammanaidu Krishnaraj, Saravanan. “Effects of fire on soil organic carbon dynamics in Eucalyptus forests of south-eastern Australia.” 2014. Doctoral Dissertation, University of Melbourne. Accessed January 27, 2021.
http://hdl.handle.net/11343/40982.
MLA Handbook (7th Edition):
Jangammanaidu Krishnaraj, Saravanan. “Effects of fire on soil organic carbon dynamics in Eucalyptus forests of south-eastern Australia.” 2014. Web. 27 Jan 2021.
Vancouver:
Jangammanaidu Krishnaraj S. Effects of fire on soil organic carbon dynamics in Eucalyptus forests of south-eastern Australia. [Internet] [Doctoral dissertation]. University of Melbourne; 2014. [cited 2021 Jan 27].
Available from: http://hdl.handle.net/11343/40982.
Council of Science Editors:
Jangammanaidu Krishnaraj S. Effects of fire on soil organic carbon dynamics in Eucalyptus forests of south-eastern Australia. [Doctoral Dissertation]. University of Melbourne; 2014. Available from: http://hdl.handle.net/11343/40982

Indian Institute of Science
3.
Gautam, Rekha.
Vibrational Microspectroscopic Studies of Biomedical Conditions Using Model Systems.
Degree: PhD, Faculty of Science, 2018, Indian Institute of Science
URL: http://etd.iisc.ac.in/handle/2005/3478
► Over the last century, despite enormous advancements in biomedical research and the development of sophisticated analytical instruments many diseases continue to be a burden on…
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▼ Over the last century, despite enormous advancements in biomedical research and the development of sophisticated analytical instruments many diseases continue to be a burden on humankind particularly on the aged. This is because of a lack of complete understanding of the pathogenesis and specific therapies. Due to the complexity involved, we need to explore all facets of diagnosis and therapies. Therefore, there is a requirement for different strategies to combat these diseases. A quick diagnosis is the primary step towards improving treatment and increasing the chance of survival. To realize this goal we entail to monitor multiple biomarkers which will also help us to understand the progression of disease. Mid-Infrared (
MIR) and Raman spectroscopic techniques are well established analytical methods to understand the molecular structure and chemical composition of heterogeneous systems. These techniques are rapid, non-destructive and offer multiple component analysis (global/multiplex) in a single measurement without any labels. Importantly, biological materials like proteins, carbohydrates, lipids, nucleic acids etc. have unique structures and therefore we can obtain unique spectral fingerprints of these molecules in different physiological and pathological conditions. This will provide a potential route to obtain diagnostic markers for diseases. Also, to improve the ability to diagnose and treat human diseases much more efficiently, understanding the mechanisms involved in the progression of disease is necessary. It would be time consuming and often unethical to perform these studies directly on humans. Therefore, there is a need for model organisms to explore the complexity of various diseases. A model organism is an animal, plant or microbe that is being studied to understand a range of biological phenomena. They should meet certain criteria such as short life cycles, easy to breed and maintain in large numbers under laboratory conditions, and the data generated through use of the model should be applicable to other higher organisms like humans. The microbial system, mouse, rat, Drosophila (fruitfly), C Elegans (nematode worm) and zebrafish are being used extensively for this purpose. The most adaptable organisms to study diseases in humans are the mice as they share almost 99% of their genes with humans. Mice are similar to humans in most physiological and pathological features such as nervous, cardiovascular, immune, liver etc. In addition to mice, Drosophila melanogaster (fruitfly) has been used for years as an attractive model organism to understand the mechanisms of underlying human diseases. This is because 75% of human disease genes have counterparts in Drosophila and it meets the above mentioned criteria to be a model organism. It also plays an important role
for studying genetics and development biology. The average life span of Drosophila is 60-80 days; therefore it is a suitable model to study age related diseases.
In the present thesis, the ability to probe low-micrometer domains using Raman and…
Advisors/Committee Members: Umapathy, Siva (advisor).
Subjects/Keywords: Vibrational Microspectroscopy; Raman Spectroscopy; Fourier Transform Infrared Spectroscopy; Biomedical Vibrational Spectroscopy; Mid-Infrared (MIR) Spectroscopy; Raman Microspectrometer; Biomedical Vibrational Spectroscopy; Surface Enhanced Raman Spectroscopy; Biomedical Engineering; Cardio-myopathy Phenotypes; Inorganic and Physical Chemistry
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❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Gautam, R. (2018). Vibrational Microspectroscopic Studies of Biomedical Conditions Using Model Systems. (Doctoral Dissertation). Indian Institute of Science. Retrieved from http://etd.iisc.ac.in/handle/2005/3478
Chicago Manual of Style (16th Edition):
Gautam, Rekha. “Vibrational Microspectroscopic Studies of Biomedical Conditions Using Model Systems.” 2018. Doctoral Dissertation, Indian Institute of Science. Accessed January 27, 2021.
http://etd.iisc.ac.in/handle/2005/3478.
MLA Handbook (7th Edition):
Gautam, Rekha. “Vibrational Microspectroscopic Studies of Biomedical Conditions Using Model Systems.” 2018. Web. 27 Jan 2021.
Vancouver:
Gautam R. Vibrational Microspectroscopic Studies of Biomedical Conditions Using Model Systems. [Internet] [Doctoral dissertation]. Indian Institute of Science; 2018. [cited 2021 Jan 27].
Available from: http://etd.iisc.ac.in/handle/2005/3478.
Council of Science Editors:
Gautam R. Vibrational Microspectroscopic Studies of Biomedical Conditions Using Model Systems. [Doctoral Dissertation]. Indian Institute of Science; 2018. Available from: http://etd.iisc.ac.in/handle/2005/3478
4.
Rammal, Abbas.
Mathématiques appliquées et traitement du signal pour l’évaluation de la dégradation de la biomasse lignocellulosique : Applied Mathematics and signal processing for the study of the evolution of plant litter during the biodegradation process.
Degree: Docteur es, Génie informatique, automatique et traitement du signal, 2016, Reims
URL: http://www.theses.fr/2016REIMS033
► Dans cette thèse nous proposons de mettre en œuvre des méthodes des mathématiques appliquées et du traitement du signal pour l’étude à partir de spectres…
(more)
▼ Dans cette thèse nous proposons de mettre en œuvre des méthodes des mathématiques appliquées et du traitement du signal pour l’étude à partir de spectres infrarouges (IR) de l’évolution des litières végétales au cours du processus de biodégradation. Nous présentons tout d’abord une nouvelle méthode de classification floue fondée sur une optimisation de type non supervisée, basée sur le facteur de covariance qui permet de classer des données IR de forme sphérique ou non sphérique afin d’identifier les méthodes de prétraitement et de choix de gammes spectrales les mieux adaptées. Nous développons des outils mathématiques et des algorithmes innovants permettant de combiner des informations spectrales moyen IR (MIR) et proche IR (MIR) afin d’identifier des marqueurs spectroscopiques discriminants de résidus lignocellulosiques en fonction de leur niveau de dégradation. Pour cela, nous proposons une méthode d'optimisation stochastique basée sur un algorithme génétique avec paramètres adaptés. Nous montrons que l’analyse conjoints des spectres MIR et NIR fusionnés par le produit extérieur permet de mieux discriminer la biomasse lignocellulosique au cours du processus de dégradation qu’un traitement séparé. Nous proposons ensuite une nouvelle approche d’optimisation non linéaire basée sur la sélection d’un vecteur qui met en évidence les poids des bandes spectrales. Enfin, nous développons une méthode de modélisation mathématique basée sur l’extension de l’algorithme AG-PLS en combinant les informations spectrales MIR et NIR par le produit extérieur (OP-AG-PLS). Cette méthode permet d’améliorer les performances de prédiction de l’état de dégradation de la biomasse.
In this thesis we propose to implement methods of applied mathematics and signal processing for the study of the evolution of plant biomass during the biodegradation process. The degradation of plant biomass is identified by FTIR spectroscopy, particularly in the MIR and NIR ranges. We proposed a new unsupervised classification method of Fuzzy C-Means based on the covariance factor to classify the IR data with spherical and not spherical form to identify the pre-treatment methods and the choice of spectral ranges that are the best adapted for our study. We have developed mathematical tools and innovative algorithms to combine these spectral information and identifying infrared spectroscopic markers that are discriminative in the lignocellulosic residues according to their level of degradation. For this, we have proposed a stochastic optimization method based on a genetic algorithm by choosing the appropriate parameters. We have shown that the joint analysis of the MIR and NIR spectra by the outer product (OP) provides better results than the separate analysis for the discrimination of the lignocellulosic biomass during the degradation process. Then, we proposed a new nonlinear optimization approach based on the built of vector which highlights the weight of spectral bands. Finally, we have developed a mathematical modelisation based on the extension of the…
Advisors/Committee Members: Vrabie, Valeriu (thesis director).
Subjects/Keywords: Classification non supervisée; Optimisation non linéaire; Algorithme génétique; Modélisation mathématique; Spectroscopie MIR - NIR; Extraction des bandes spectrales discriminantes; Unsupervised classification; Nonlinear optimization; Genetic Algorithm; Mathematical modeling; Spectroscopy MIR- NIR; Extracting spectroscopic markers
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❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Rammal, A. (2016). Mathématiques appliquées et traitement du signal pour l’évaluation de la dégradation de la biomasse lignocellulosique : Applied Mathematics and signal processing for the study of the evolution of plant litter during the biodegradation process. (Doctoral Dissertation). Reims. Retrieved from http://www.theses.fr/2016REIMS033
Chicago Manual of Style (16th Edition):
Rammal, Abbas. “Mathématiques appliquées et traitement du signal pour l’évaluation de la dégradation de la biomasse lignocellulosique : Applied Mathematics and signal processing for the study of the evolution of plant litter during the biodegradation process.” 2016. Doctoral Dissertation, Reims. Accessed January 27, 2021.
http://www.theses.fr/2016REIMS033.
MLA Handbook (7th Edition):
Rammal, Abbas. “Mathématiques appliquées et traitement du signal pour l’évaluation de la dégradation de la biomasse lignocellulosique : Applied Mathematics and signal processing for the study of the evolution of plant litter during the biodegradation process.” 2016. Web. 27 Jan 2021.
Vancouver:
Rammal A. Mathématiques appliquées et traitement du signal pour l’évaluation de la dégradation de la biomasse lignocellulosique : Applied Mathematics and signal processing for the study of the evolution of plant litter during the biodegradation process. [Internet] [Doctoral dissertation]. Reims; 2016. [cited 2021 Jan 27].
Available from: http://www.theses.fr/2016REIMS033.
Council of Science Editors:
Rammal A. Mathématiques appliquées et traitement du signal pour l’évaluation de la dégradation de la biomasse lignocellulosique : Applied Mathematics and signal processing for the study of the evolution of plant litter during the biodegradation process. [Doctoral Dissertation]. Reims; 2016. Available from: http://www.theses.fr/2016REIMS033

Université de Lorraine
5.
Ajib, Budour.
Contribution à la modélisation de la qualité de l'orge et du malt pour la maîtrise du procédé de maltage : Modeling contribution of barley and malt quality for the malting process control.
Degree: Docteur es, Génie des procédés et des produits, 2013, Université de Lorraine
URL: http://www.theses.fr/2013LORR0315
► Dans un marché en permanente progression et pour répondre aux besoins des brasseurs en malt de qualité, la maîtrise du procédé de maltage est indispensable.…
(more)
▼ Dans un marché en permanente progression et pour répondre aux besoins des brasseurs en malt de qualité, la maîtrise du procédé de maltage est indispensable. La qualité du malt est fortement dépendante des conditions opératoires, en particulier des conditions de trempe, mais également de la qualité de la matière première : l'orge. Dans cette étude, nous avons établi des modèles polynomiaux qui mettent en relation les conditions opératoires et la qualité du malt. Ces modèles ont été couplés à nos algorithmes génétiques et nous ont permis de déterminer les conditions optimales de maltage, soit pour atteindre une qualité ciblée de malt (friabilité), soit pour permettre un maltage à faible teneur en eau (pour réduire la consommation en eau et maîtriser les coûts environnementaux de production) tout en conservant une qualité acceptable de malt. Cependant, la variabilité de la matière première est un facteur limitant de notre approche. Les modèles établis sont en effet très sensibles à l'espèce d'orge (printemps, hiver) ou encore à la variété d'orge utilisée. Les modèles sont surtout très dépendants de l'année de récolte. Les variations observées sur les propriétés d'une année de récolte à une autre sont mal caractérisées et ne sont donc pas intégrées dans nos modèles. Elles empêchent ainsi de capitaliser l'information expérimentale au cours du temps. Certaines propriétés structurelles de l'orge (porosité, dureté) ont été envisagées comme nouveaux facteurs pour mieux caractériser la matière première mais ils n'ont pas permis d'expliquer les variations observés en malterie.Afin de caractériser la matière première, 394 échantillons d'orge issus de 3 années de récolte différentes 2009-2010-2011 ont été analysés par spectroscopie MIR. Les analyses ACP ont confirmé l'effet notable des années de récolte, des espèces, des variétés voire des lieux de culture sur les propriétés de l'orge. Une régression PLS a permis, pour certaines années et pour certaines espèces, de prédire les teneurs en protéines et en béta-glucanes de l'orge à partir des spectres MIR. Cependant, ces résultats, pourtant prometteurs, se heurtent toujours à la variabilité. Ces nouveaux modèles PLS peuvent toutefois être exploités pour mettre en place des stratégies de pilotage du procédé de maltage à partir de mesures spectroscopiques MIR
In a continuously growing market and in order to meet the needs of Brewers in high quality malt, control of the malting process is a great challenge. Malt quality is highly dependent on the malting process operating conditions, especially on the steeping conditions, but also the quality of the raw material: barley. In this study, we established polynomial models that relate the operating conditions and the malt quality. These models have been coupled with our genetic algorithms to determine the optimal steeping conditions, either to obtain a targeted quality of malt (friability), or to allow a malting at low water content while maintaining acceptable quality of malt (to reduce water consumption and control the environmental…
Advisors/Committee Members: Fick, Michel (thesis director), Fournier, Frantz (thesis director).
Subjects/Keywords: Orge; Maltage; Moyen infrarouge spectroscopie (MIR); Régression multiliniaiare (MLR); Analyses en composantes principales (PCA); Régression en moindre carrée partielle (PLS); Barley; Malting; Middle infrared spectroscopy (MIR); Multilinear regression (MLR); Principal component analysis (PCA); Partial least square regression (PLS)
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❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Ajib, B. (2013). Contribution à la modélisation de la qualité de l'orge et du malt pour la maîtrise du procédé de maltage : Modeling contribution of barley and malt quality for the malting process control. (Doctoral Dissertation). Université de Lorraine. Retrieved from http://www.theses.fr/2013LORR0315
Chicago Manual of Style (16th Edition):
Ajib, Budour. “Contribution à la modélisation de la qualité de l'orge et du malt pour la maîtrise du procédé de maltage : Modeling contribution of barley and malt quality for the malting process control.” 2013. Doctoral Dissertation, Université de Lorraine. Accessed January 27, 2021.
http://www.theses.fr/2013LORR0315.
MLA Handbook (7th Edition):
Ajib, Budour. “Contribution à la modélisation de la qualité de l'orge et du malt pour la maîtrise du procédé de maltage : Modeling contribution of barley and malt quality for the malting process control.” 2013. Web. 27 Jan 2021.
Vancouver:
Ajib B. Contribution à la modélisation de la qualité de l'orge et du malt pour la maîtrise du procédé de maltage : Modeling contribution of barley and malt quality for the malting process control. [Internet] [Doctoral dissertation]. Université de Lorraine; 2013. [cited 2021 Jan 27].
Available from: http://www.theses.fr/2013LORR0315.
Council of Science Editors:
Ajib B. Contribution à la modélisation de la qualité de l'orge et du malt pour la maîtrise du procédé de maltage : Modeling contribution of barley and malt quality for the malting process control. [Doctoral Dissertation]. Université de Lorraine; 2013. Available from: http://www.theses.fr/2013LORR0315

KTH
6.
Farshchian, Mona.
Structural elucidation of mRNA(Sirt1)-microRNA 34a complex.
Degree: Education and Communication in Engineering Science (ECE), 2015, KTH
URL: http://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-168623
► The aim of this thesis is to understand RNA-RNA interactions steering cellular functions, as in the case of this thesis the structure of mRNA(Sirt1)…
(more)
▼ The aim of this thesis is to understand RNA-RNA interactions steering cellular functions, as in the case of this thesis the structure of mRNA(Sirt1) in complex with microRNA-34a (miR-34a). MiR-34a regulates the cancer protein p53 via Sirt1 modulation. This work will be the basis for future drug design and the understanding of misguided regulation in cancer. The miR-34a binds to the mRNA(Sirt1) 3’ untranslated region (3’-UTR) and will either inhibit the translation of the protein Sirtuin 1 by capturing its mRNA or by degrading it. p53, a key activator of miR-34a, prevents cancer development by inducing programmed cell death (apoptosis) on cells with DNA damage. In contrast, the protein Sirtuin 1 (Sirt1) has been shown to help cells with DNA damage to survive by down regulating the activity of protein p53 and will therefore increase the risk of cancer development. Studying the interaction between the mRNA(Sirt1) and miR-34a can present valuable information on the structure of the complex as well as the mode miR-34a uses to inhibit translation of mRNA(Sirt1) leading to down regulation of protein Sirtuin 1 and therefore prevent cancer development. For the elucidation of this question different biochemical and biophysical methods were applied, such as in vitro transcription, gel electrophoresis, RNA purification with gel, crush & soak and Cicular Dichroism (CD) melting studies. For this thesis work, the target sequence in mRNA(Sirt1) was optimized and purified so melting studies could be carried out. For future structural characterization using Nuclear Magnetic Resonance (NMR) studies with the miR-34a also produced in the lab. The mRNA(Sirt1) target sequence was produced and purified with the final yield of 0.02%. The results show that the sequence is highly ATP dependent and suggest the ratio between the nucleotides ATP/CTP to be 1:2. Low yield of purified mRNA(Sirt1) was received and still contained some impurities, which imply that another method than crush & soak should be used when purifying. The results, indicate that High-Preformance Liquid Chromatography (HPLC) might be a better solution for the pufication process. The melting profiles done on mRNA(Sirt1) show that the secondary structures decrease with an increase in temperature. Accroding to the results, the mRNA(Sirt1) sequence is folded in room temperature, though not very stable. The wavelength which provided the best resolution was at 268 nm and the melting point of mRNA(Sirt1) was determined to 44 °C. This thesis also contains an educational part, where an educational material was provided and testing was conducted for the subject Chemistry 2 for students age 18 and the material was evaluated with qualitative methods together with pedagogical methods. The study showed that the student can develope the different abilities stated in the curriculum with the material created. The results also showed that the students preferably choose cultural arguments when dicussing socio scientific question, rather than economical,…
Subjects/Keywords: mRNA(Sirt1); miR-34a; in vitro transcription; gel electrophoresis; CD spectroscopy; NMR; cancer regulation via p53; HPLC; mRNA(Sirt1); miR-34a; in vitro transkription; gel elektroforesis; CD spektroskopi; NMR; cancer regulering via p53; HPLC
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❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
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APA (6th Edition):
Farshchian, M. (2015). Structural elucidation of mRNA(Sirt1)-microRNA 34a complex. (Thesis). KTH. Retrieved from http://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-168623
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Farshchian, Mona. “Structural elucidation of mRNA(Sirt1)-microRNA 34a complex.” 2015. Thesis, KTH. Accessed January 27, 2021.
http://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-168623.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Farshchian, Mona. “Structural elucidation of mRNA(Sirt1)-microRNA 34a complex.” 2015. Web. 27 Jan 2021.
Vancouver:
Farshchian M. Structural elucidation of mRNA(Sirt1)-microRNA 34a complex. [Internet] [Thesis]. KTH; 2015. [cited 2021 Jan 27].
Available from: http://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-168623.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Farshchian M. Structural elucidation of mRNA(Sirt1)-microRNA 34a complex. [Thesis]. KTH; 2015. Available from: http://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-168623
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
7.
Luzinova, Yuliya.
Mid-infrared sensors for hydrocarbon analysis in extreme environments.
Degree: PhD, Chemistry and Biochemistry, 2010, Georgia Tech
URL: http://hdl.handle.net/1853/41156
► A number of MIR sensing platforms and methods were developed in this research work demonstrating potential applicability of MIR spectroscopy for studying hydrocarbon systems in…
(more)
▼ A number of
MIR sensing platforms and methods were developed in this research work demonstrating potential applicability of
MIR spectroscopy for studying hydrocarbon systems in extreme environments.
First of all, the quantitative determination of the diamondoid compound adamantane in organic media utilizing IR-ATR
spectroscopy at waveguide surfaces was established. The developed analytical strategy further enabled the successful detection of adamantane in real world crude oil samples. These reported efforts provide a promising outlook for detection and monitoring of diamondoid constituents in naturally occurring crudes and petroleum samples.
IR-ATR
spectroscopy was further utilized for evaluating and characterizing distribution, variations, and origin of carbonate minerals within sediment formations surrounding a hydrocarbon seep site - MC 118 in the Gulf of Mexico. An analytical model for direct detection of 13C-depleted authigenic carbonates associated with cold seep ecosystems was constructed. Potential applicability of IR-ATR
spectroscopy as direct on-ship - and in future in situ - analytical tool for characterizing hydrocarbon seep sites was demonstrated.
MIR evanescent field absorption
spectroscopy was also utilized to expand the understanding on the role of surfactants during gas hydrate formation at surfaces. This experimental method allowed detailed spectroscopic observations of detergent-related surface processes during SDS mediated gas hydrate formation. The obtained IR data enabled proposing a mechanism by which SDS decreases the induction time for hydrate nucleation, and promotes hydrate formation. Potential of
MIR fiberoptic evanescent field
spectroscopy for studying surface effects during gas hydrate nucleation and growth was demonstrated.
Next, quantifying trace amounts of water content in hexane using
MIR evanescent field absorption
spectroscopy is presented. The improvement in sensitivity and of limit of detection was obtained by coating an optical fiber with layer of a hydrophilic polymer. The application of the polymer layer has enabled the on-line
MIR detection of water in hexane at low ppm levels. These results indicate that the
MIR evanescent filed
spectroscopy method shows potential for in-situ detection and monitoring of water in industrial oils and petroleum products.
Finally, quantification of trace amounts of oil content in water using
MIR evanescent field absorption
spectroscopy is reported. Unmodified and modified with grafted hydrophobic polymer layer silver halide optical fibers were employed for the measurements. The surface modification of the fiber has enabled the on-line
MIR analysis of crude oil in water at the low ppb level. Potential application of
MIR fiber-optic evanescent field
spectroscopy using polymer modified waveguides toward in-situ low level detection of crude oil in open waters was demonstrated.
Advisors/Committee Members: Boris Mizaikoff (Committee Chair), Thomas Orlando (Committee Co-Chair), Facundo Fernandez (Committee Member), Jiri Janata (Committee Member), Oliver Brand (Committee Member), Seong-Soo Kim (Committee Member).
Subjects/Keywords: MIR spectroscopy; IR-ATR spectroscopy; Diamondoids; Gas hydrates; Water-crude oil emulsions; MIR sensing platforms; Hydrocarbons; Infrared detectors; Infrared technology
…marine sediments
A-2: MIR Fiberoptic evanescent field spectroscopy for analysis of
water… …research
work demonstrating potential applicability of MIR spectroscopy for studying
hydrocarbon… …characterizing hydrocarbon seep
sites was demonstrated.
MIR evanescent field absorption spectroscopy… …results indicate that the MIR evanescent filed
spectroscopy method shows potential for in-situ… …Potential application of MIR fiberoptic evanescent field spectroscopy using polymer modified…
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❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Luzinova, Y. (2010). Mid-infrared sensors for hydrocarbon analysis in extreme environments. (Doctoral Dissertation). Georgia Tech. Retrieved from http://hdl.handle.net/1853/41156
Chicago Manual of Style (16th Edition):
Luzinova, Yuliya. “Mid-infrared sensors for hydrocarbon analysis in extreme environments.” 2010. Doctoral Dissertation, Georgia Tech. Accessed January 27, 2021.
http://hdl.handle.net/1853/41156.
MLA Handbook (7th Edition):
Luzinova, Yuliya. “Mid-infrared sensors for hydrocarbon analysis in extreme environments.” 2010. Web. 27 Jan 2021.
Vancouver:
Luzinova Y. Mid-infrared sensors for hydrocarbon analysis in extreme environments. [Internet] [Doctoral dissertation]. Georgia Tech; 2010. [cited 2021 Jan 27].
Available from: http://hdl.handle.net/1853/41156.
Council of Science Editors:
Luzinova Y. Mid-infrared sensors for hydrocarbon analysis in extreme environments. [Doctoral Dissertation]. Georgia Tech; 2010. Available from: http://hdl.handle.net/1853/41156

Universidade do Estado do Rio de Janeiro
8.
Arnaldo Peixoto da Silva.
Acoplamento de técnicas espectrométricas com métodos quimiométricos de classificação e calibração multivariada em alimentos.
Degree: Master, 2011, Universidade do Estado do Rio de Janeiro
URL: http://www.bdtd.uerj.br/tde_busca/arquivo.php?codArquivo=3556
;
► Este trabalho de pesquisa descreve três estudos de utilização de métodos quimiométricos para a classificação e caracterização de óleos comestíveis vegetais e seus parâmetros de…
(more)
▼ Este trabalho de pesquisa descreve três estudos de utilização de métodos quimiométricos para a classificação e caracterização de óleos comestíveis vegetais e seus parâmetros de qualidade através das técnicas de espectrometria de absorção molecular no infravermelho médio com transformada de Fourier e de espectrometria no infravermelho próximo, e o monitoramento da qualidade e estabilidade oxidativa do iogurte usando espectrometria de fluorescência molecular.
O primeiro e segundo estudos visam à classificação e caracterização de parâmetros de qualidade de óleos comestíveis vegetais utilizando espectrometria no infravermelho médio com transformada de Fourier (FT-MIR) e no infravermelho próximo (NIR).
O algoritmo de Kennard-Stone foi usado para a seleção do conjunto de validação após análise de componentes principais (PCA). A discriminação entre os óleos de canola, girassol, milho e soja foi investigada usando SVM-DA, SIMCA e PLS-DA. A predição dos parâmetros de qualidade, índice de refração e densidade relativa dos óleos, foi investigada usando os métodos de calibração multivariada dos mínimos quadrados parciais (PLS), iPLS e SVM para os dados de FT-MIR e NIR. Vários tipos de pré-processamentos, primeira derivada, correção do sinal multiplicativo (MSC), dados centrados na média, correção do sinal ortogonal (OSC) e variação normal padrão (SNV) foram utilizados, usando a raiz quadrada do erro médio quadrático de validação cruzada (RMSECV) e de predição (RMSEP) como parâmetros de avaliação. A metodologia desenvolvida para determinação de índice de refração e densidade relativa e classificação dos óleos vegetais é rápida e direta.
O terceiro estudo visa à avaliação da estabilidade oxidativa e qualidade do iogurte armazenado a 4C submetido à luz direta e mantido no escuro, usando a análise dos fatores paralelos (PARAFAC) na luminescência exibida por três fluoróforos presentes no iogurte, onde pelo menos um deles está fortemente relacionado com as condições de armazenamento. O sinal fluorescente foi identificado pelo espectro de emissão e excitação das substâncias fluorescentes puras, que foram sugeridas serem vitamina A, triptofano e riboflavina.
Modelos de regressão baseados nos escores do PARAFAC para a riboflavina foram desenvolvidos usando os escores obtidos no primeiro dia como variável dependente e os escores obtidos durante o armazenamento como variável independente. Foi visível o decaimento da curva analítica com o decurso do tempo da experimentação. Portanto, o teor de riboflavina pode ser considerado um bom indicador para a estabilidade do iogurte. Assim, é possível concluir que a espectroscopia de fluorescência combinada com métodos quimiométricos é um método rápido para monitorar a estabilidade oxidativa e a qualidade do iogurte
This research work describes three studies of chemometric methods employed for the classification and characterization of edible oils and its quality parameters through Fourier Transform mid infrared spectroscopy and near infrared spectroscopy, and for the monitoring the oxidative…
Advisors/Committee Members: Ayres Guimarães Dias, Cristiane Assumpção Henriques, Paulo Henrique Março, Aderval Severino Luna.
Subjects/Keywords: Calibração multivariada de primeira ordem; TECNOLOGIA QUIMICA; infravermelho próximo (NIR); infravermelho médio com transformada de Fourier (FT-MIR); análise discriminatória; óleos comestíveis vegetais; iogurte; First-order multivariate calibration methods; near infrared spectroscopy; Fourier Transform mid infrared spectroscopy; classification chemometric methods; yogurt; edible oils
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❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Silva, A. P. d. (2011). Acoplamento de técnicas espectrométricas com métodos quimiométricos de classificação e calibração multivariada em alimentos. (Masters Thesis). Universidade do Estado do Rio de Janeiro. Retrieved from http://www.bdtd.uerj.br/tde_busca/arquivo.php?codArquivo=3556 ;
Chicago Manual of Style (16th Edition):
Silva, Arnaldo Peixoto da. “Acoplamento de técnicas espectrométricas com métodos quimiométricos de classificação e calibração multivariada em alimentos.” 2011. Masters Thesis, Universidade do Estado do Rio de Janeiro. Accessed January 27, 2021.
http://www.bdtd.uerj.br/tde_busca/arquivo.php?codArquivo=3556 ;.
MLA Handbook (7th Edition):
Silva, Arnaldo Peixoto da. “Acoplamento de técnicas espectrométricas com métodos quimiométricos de classificação e calibração multivariada em alimentos.” 2011. Web. 27 Jan 2021.
Vancouver:
Silva APd. Acoplamento de técnicas espectrométricas com métodos quimiométricos de classificação e calibração multivariada em alimentos. [Internet] [Masters thesis]. Universidade do Estado do Rio de Janeiro; 2011. [cited 2021 Jan 27].
Available from: http://www.bdtd.uerj.br/tde_busca/arquivo.php?codArquivo=3556 ;.
Council of Science Editors:
Silva APd. Acoplamento de técnicas espectrométricas com métodos quimiométricos de classificação e calibração multivariada em alimentos. [Masters Thesis]. Universidade do Estado do Rio de Janeiro; 2011. Available from: http://www.bdtd.uerj.br/tde_busca/arquivo.php?codArquivo=3556 ;

University of Otago
9.
Rooney, Jeremy.
Vibrational Spectroscopy and Multivariate Analyses for the Detection of Adulterated Weightloss Products
.
Degree: University of Otago
URL: http://hdl.handle.net/10523/5414
► The feasibility of vibrational spectroscopic techniques for the detection and semi-quantification of anorectic and laxative pharmaceuticals used as adulterants in herbal medicines was investigated. The…
(more)
▼ The feasibility of vibrational spectroscopic techniques for the detection and semi-quantification of anorectic and laxative pharmaceuticals used as adulterants in herbal medicines was investigated. The vibrational spectroscopic techniques used were mid-infrared (
MIR), near infrared (NIR) and Raman. Each of these may allow routine screening of herbal medicines that would otherwise not undergo analyses at New Zealand's borders. All three techniques are rapid, cost-effective, non-destructive and can be automated.
A high pressure liquid chromatography (HPLC) method was designed, validated and utilised to obtain reference values to allow multivariate models to be constructed from the spectroscopic measurements. FT-Raman
spectroscopy was determined unsuitable during sample measurements due to strong emission and sample burning. Conversely, FT-
MIR and FT-NIR were pursued further. Principal component analysis (PCA) models showed separation of scores based on adulteration for the
MIR and NIR datasets. Consequently, support vector machine (SVM) models were formed from the training sets' PCA scores and the performance of these models was then gauged by test set prediction. The accuracies for SVM classification ranged between 97-100%. Lastly,
MIR and NIR training sets were used for the creation of partial least squares (PLS) models for semi-quantification of the two adulterants (phenolphthalein and sibutramine hydrochloride monohydrate). The test set predictive performances for the
MIR models were high and were apposite for semi-quantification of the adulterants necessary to determine consumer risk; the NIR models showed a lesser performance and had larger root mean squared error of prediction (RMSEP) values. It was concluded
MIR spectroscopy, when combined with PCA, SVM and PLS, is suitable for detection and semi-quantification of the adulterants in herbal medicines.
Advisors/Committee Members: Gordon, Keith C (advisor).
Subjects/Keywords: MIR;
NIR;
Raman;
Principal Component Analysis;
Support Vector Machines;
Partial Least Squares Regression;
Anorectics;
Laxatives;
Adulterants;
Vibrational Spectroscopy
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❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Rooney, J. (n.d.). Vibrational Spectroscopy and Multivariate Analyses for the Detection of Adulterated Weightloss Products
. (Masters Thesis). University of Otago. Retrieved from http://hdl.handle.net/10523/5414
Note: this citation may be lacking information needed for this citation format:
No year of publication.
Chicago Manual of Style (16th Edition):
Rooney, Jeremy. “Vibrational Spectroscopy and Multivariate Analyses for the Detection of Adulterated Weightloss Products
.” Masters Thesis, University of Otago. Accessed January 27, 2021.
http://hdl.handle.net/10523/5414.
Note: this citation may be lacking information needed for this citation format:
No year of publication.
MLA Handbook (7th Edition):
Rooney, Jeremy. “Vibrational Spectroscopy and Multivariate Analyses for the Detection of Adulterated Weightloss Products
.” Web. 27 Jan 2021.
Note: this citation may be lacking information needed for this citation format:
No year of publication.
Vancouver:
Rooney J. Vibrational Spectroscopy and Multivariate Analyses for the Detection of Adulterated Weightloss Products
. [Internet] [Masters thesis]. University of Otago; [cited 2021 Jan 27].
Available from: http://hdl.handle.net/10523/5414.
Note: this citation may be lacking information needed for this citation format:
No year of publication.
Council of Science Editors:
Rooney J. Vibrational Spectroscopy and Multivariate Analyses for the Detection of Adulterated Weightloss Products
. [Masters Thesis]. University of Otago; Available from: http://hdl.handle.net/10523/5414
Note: this citation may be lacking information needed for this citation format:
No year of publication.
10.
Putaud, Thomas.
Études infrarouges des isotopes H216O, H217O et H218O de la molécule d'eau à basse température : dynamique de conversion de spin nucléaire en matrice de gaz rare et rapport ortho-para de l'eau dans la Barre d'Orion : Infrared studies of H216O, H217O and H218O water isotopes at low temperature : nuclear spin conversion dynamic in rare gas matrix and water ortho-to-para ratio in the Orion Bar.
Degree: Docteur es, Physique, 2019, Sorbonne université
URL: http://www.theses.fr/2019SORUS321
► Ce travail s'attache à l'étude de la répartition des populations des isomères de spin nucléaire des isotopes de l'eau dans le milieu interstellaire et en…
(more)
▼ Ce travail s'attache à l'étude de la répartition des populations des isomères de spin nucléaire des isotopes de l'eau dans le milieu interstellaire et en matrice de gaz rare. Les intensités des raies d'émission infrarouge de l'eau dans la Barre d'Orion ont été analysées avec le modèle de transfert radiatif du code PDR de Meudon. L'adsorption des molécules d'eau à la surface des grains interstellaires sur la chimie de l'eau a été étudiée et mène à une augmentation de la production de l'eau en phase gazeuse pour reproduire les observations. Un OPR de 2.8 à l'équilibre thermodynamique à 36 K s'est révélé correspondre aux observations. L'influence du confinement de l'eau en matrice de gaz rare sur les temps de conversion de spin nucléaire a été étudiée. En revisitant l'attribution des transitions de l'eau en matrice de gaz rare dans l'infrarouge lointain, un confinement plus fort en matrice d'argon que de krypton est déduit du modèle de couplage rotation-translation. La structure rotationnelle (énergies et fonctions d'onde) des isotopes de l'eau en matrices d'argon et de krypton est établie révélant des mélanges d'états de rotation et de translation. Les variations des temps de conversion en fonction de la température sont reproduites par des couplages magnétiques intramoléculaire de type spin-rotation. Les canaux de conversion entre les états rotationnels des deux isomères de spin sont discutés. Enfin, la faisabilité d'un enrichissement en un isomère de spin par pompage optique infrarouge est adressée et l'efficacité de cette technique est limitée par une relaxation vibrationnelle rapide en matrice de gaz rare.
The purpose of this work is to study the variations of the ortho-to-para ratio of water isotopes in the interstellar medium and rare gas matrix. The intensities of the water infrared emission lines observed in the Orion Bar were analyzed using the radiative transfer model of the Meudon PDR Code. The water chemistry modifications due to the adsorption of water molecules on interstellar grains were investigated and lead to a strong water depletion which has to be balanced by enhancing water gas phase production in order to retrieve the observed water line intensities. An agreement between the observed and predicted intensities is obtained for an OPR of 2.8 at the thermal equilibrium at 36 K. The role of water confinement on the nuclear spin conversion characteristic time is investigated. Considering a new water line assignment in the far infrared and rotation-translation coupling model, water molecules are more confined in argon matrix than in krypton one. The rotational structure of water isotopes in argon and krypton matrices is derived showing perturbations of the asymmetric rotator wave functions with a mixing of rotation and translation states. The variations of the nuclear spin conversion time with the temperature are retrieved by intramolecular magnetic interactions through spin-rotation coupling. Furthermore considering phonon processes, the nuclear spin conversion channels between nuclear spin isomer…
Advisors/Committee Members: Michaut, Xavier (thesis director).
Subjects/Keywords: Eau; Isomères de spin nucléaire; Barre d'Orion; Matrice de gaz rare; Spectroscopie MIR et FIR; Conversion de spin nucléaire; Enrichissement; Water; Nuclear spin isomers; Orion Bar; Rare gas matrix; Far- and mid-infrared spectroscopy; Nuclear spin conversion; Enrichment; 531.34; 546.22
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❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Putaud, T. (2019). Études infrarouges des isotopes H216O, H217O et H218O de la molécule d'eau à basse température : dynamique de conversion de spin nucléaire en matrice de gaz rare et rapport ortho-para de l'eau dans la Barre d'Orion : Infrared studies of H216O, H217O and H218O water isotopes at low temperature : nuclear spin conversion dynamic in rare gas matrix and water ortho-to-para ratio in the Orion Bar. (Doctoral Dissertation). Sorbonne université. Retrieved from http://www.theses.fr/2019SORUS321
Chicago Manual of Style (16th Edition):
Putaud, Thomas. “Études infrarouges des isotopes H216O, H217O et H218O de la molécule d'eau à basse température : dynamique de conversion de spin nucléaire en matrice de gaz rare et rapport ortho-para de l'eau dans la Barre d'Orion : Infrared studies of H216O, H217O and H218O water isotopes at low temperature : nuclear spin conversion dynamic in rare gas matrix and water ortho-to-para ratio in the Orion Bar.” 2019. Doctoral Dissertation, Sorbonne université. Accessed January 27, 2021.
http://www.theses.fr/2019SORUS321.
MLA Handbook (7th Edition):
Putaud, Thomas. “Études infrarouges des isotopes H216O, H217O et H218O de la molécule d'eau à basse température : dynamique de conversion de spin nucléaire en matrice de gaz rare et rapport ortho-para de l'eau dans la Barre d'Orion : Infrared studies of H216O, H217O and H218O water isotopes at low temperature : nuclear spin conversion dynamic in rare gas matrix and water ortho-to-para ratio in the Orion Bar.” 2019. Web. 27 Jan 2021.
Vancouver:
Putaud T. Études infrarouges des isotopes H216O, H217O et H218O de la molécule d'eau à basse température : dynamique de conversion de spin nucléaire en matrice de gaz rare et rapport ortho-para de l'eau dans la Barre d'Orion : Infrared studies of H216O, H217O and H218O water isotopes at low temperature : nuclear spin conversion dynamic in rare gas matrix and water ortho-to-para ratio in the Orion Bar. [Internet] [Doctoral dissertation]. Sorbonne université; 2019. [cited 2021 Jan 27].
Available from: http://www.theses.fr/2019SORUS321.
Council of Science Editors:
Putaud T. Études infrarouges des isotopes H216O, H217O et H218O de la molécule d'eau à basse température : dynamique de conversion de spin nucléaire en matrice de gaz rare et rapport ortho-para de l'eau dans la Barre d'Orion : Infrared studies of H216O, H217O and H218O water isotopes at low temperature : nuclear spin conversion dynamic in rare gas matrix and water ortho-to-para ratio in the Orion Bar. [Doctoral Dissertation]. Sorbonne université; 2019. Available from: http://www.theses.fr/2019SORUS321
11.
Milligan, Alex Michael.
Application of Mid-Infrared Spectrometers in Determination
and Quantification of Trans-fatty Acid Content in Snack Foods and
Bakery Products.
Degree: MS, Food Science and Technology, 2014, The Ohio State University
URL: http://rave.ohiolink.edu/etdc/view?acc_num=osu1398786873
► Nutritional labels often under report trans-fat content. Due to the health problems associated with consumption of trans-fats, efforts must be made to ensure careful monitoring…
(more)
▼ Nutritional labels often under report trans-fat
content. Due to the health problems associated with consumption of
trans-fats, efforts must be made to ensure careful monitoring and
enforcement of current guidelines. Recent FDA press releases
indicate that trans-fat will be removed from the GRAS substances
list in the near future. If such regulation were to be enacted, it
would effectively act as a ban on all ¬trans-fats in food. The
objective of this study was to isolate and quantify trans-fat
content in a variety of local food products reporting some level of
trans-fat in the product and approximate the prevalence of
misrepresentation of trans-fat levels across several types of
foods. Isolation of trans-fatty acids from locally obtained food
products was achieved using AOAC Official Method 2000.10 and
analysis was performed using the Cary 630 portable FTIR. A standard
curve was constructed using trielaidin at varying concentrations.
Isolated trans-fats from 40 food products were analyzed; three
replicates were run for each product. Spectral data examined using
partial least squares regression (PLSR) showed very good
correlations (R²; > 0.998) for models produced using both
spectrometers. Portable ATR-
MIR spectrometers allow for increased
flexibility in set up and use while retaining the traditional
benefits of FTIR
spectroscopy such as rapid throughput, high
sensitivity, and large amounts of data per second, making it ideal
for regulatory applications and well suited to quality control
applications
Advisors/Committee Members: Rodriguez-Saona, Luis (Advisor).
Subjects/Keywords: Food Science; Trans-fat; Infrared Spectroscopy; MIR ATR-FTIR; Mid-infrared; PLSR; multivariate; Partially hydrogenated oils; edible oils; determination
…bands make MIR spectroscopy ideal for qualitative and quantitative
analyses (Li-Chan et… …spectroscopy
Spectroscopy is the study of the interaction between light and matter. When matter is… …a complex matrix. IR spectroscopy has been widely
used in the characterization… …1996; Azizian & Kramer, 2005).
1.2.2 Mid-Infrared Spectroscopy
Application of mid… …infrared spectroscopy is more sensitive to slight chemical changes in
foods than is near-infrared…
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APA (6th Edition):
Milligan, A. M. (2014). Application of Mid-Infrared Spectrometers in Determination
and Quantification of Trans-fatty Acid Content in Snack Foods and
Bakery Products. (Masters Thesis). The Ohio State University. Retrieved from http://rave.ohiolink.edu/etdc/view?acc_num=osu1398786873
Chicago Manual of Style (16th Edition):
Milligan, Alex Michael. “Application of Mid-Infrared Spectrometers in Determination
and Quantification of Trans-fatty Acid Content in Snack Foods and
Bakery Products.” 2014. Masters Thesis, The Ohio State University. Accessed January 27, 2021.
http://rave.ohiolink.edu/etdc/view?acc_num=osu1398786873.
MLA Handbook (7th Edition):
Milligan, Alex Michael. “Application of Mid-Infrared Spectrometers in Determination
and Quantification of Trans-fatty Acid Content in Snack Foods and
Bakery Products.” 2014. Web. 27 Jan 2021.
Vancouver:
Milligan AM. Application of Mid-Infrared Spectrometers in Determination
and Quantification of Trans-fatty Acid Content in Snack Foods and
Bakery Products. [Internet] [Masters thesis]. The Ohio State University; 2014. [cited 2021 Jan 27].
Available from: http://rave.ohiolink.edu/etdc/view?acc_num=osu1398786873.
Council of Science Editors:
Milligan AM. Application of Mid-Infrared Spectrometers in Determination
and Quantification of Trans-fatty Acid Content in Snack Foods and
Bakery Products. [Masters Thesis]. The Ohio State University; 2014. Available from: http://rave.ohiolink.edu/etdc/view?acc_num=osu1398786873
.