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You searched for subject:(Luminex technology). Showing records 1 – 3 of 3 total matches.

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Brno University of Technology

1. Babušíková, Lucie. Využití flowcytometrie pro multiplexové analýzy v klinické biochemii: Application of flow cytometry for multiplex analyses in clinical biochemistry.

Degree: 2019, Brno University of Technology

This thesis discusses the technique of flow cytometry for multiplex analysis and its use in conjunction with imunochemical methods. As part of this work was carried out clinical studies dealing with secondary prevention of myocardial infarction and atherosclerosis in 186 pacientů. In this time represents myocardial infarction worldwide civilizational problem. A number of possible parameters for monitoring atherosclerosis in the world is still an unresolved issue. In the practical part of this work we performed an analysis using Luminex xMAP technology for new parameters (adiponectin, resistin, osteopontin) to predict atherosclerotic disease associated with myocardial infarcion. Also we wanted to see how these parameters are changed in patients after increasing the dose of therapeutic drugs. Advisors/Committee Members: Beňovská,, Miroslava (advisor), Tremlová,, Bohuslava (referee).

Subjects/Keywords: flow cytometrie; xMAP technologie Luminex; imunoanalýza; ateroskleróza; infarkt myokardu; adiponektin; resistin; osteopontin; Lp-PLA2; myeloperoxidáza; flow cytometry; Luminex xMAP technology; immunoassay; atherosclerosis; myocardial infarction; adiponectin; resistin; osteopontin; Lp-PLA2; myeloperoxidasa

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6th Edition):

Babušíková, L. (2019). Využití flowcytometrie pro multiplexové analýzy v klinické biochemii: Application of flow cytometry for multiplex analyses in clinical biochemistry. (Thesis). Brno University of Technology. Retrieved from http://hdl.handle.net/11012/7761

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16th Edition):

Babušíková, Lucie. “Využití flowcytometrie pro multiplexové analýzy v klinické biochemii: Application of flow cytometry for multiplex analyses in clinical biochemistry.” 2019. Thesis, Brno University of Technology. Accessed September 26, 2020. http://hdl.handle.net/11012/7761.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7th Edition):

Babušíková, Lucie. “Využití flowcytometrie pro multiplexové analýzy v klinické biochemii: Application of flow cytometry for multiplex analyses in clinical biochemistry.” 2019. Web. 26 Sep 2020.

Vancouver:

Babušíková L. Využití flowcytometrie pro multiplexové analýzy v klinické biochemii: Application of flow cytometry for multiplex analyses in clinical biochemistry. [Internet] [Thesis]. Brno University of Technology; 2019. [cited 2020 Sep 26]. Available from: http://hdl.handle.net/11012/7761.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Babušíková L. Využití flowcytometrie pro multiplexové analýzy v klinické biochemii: Application of flow cytometry for multiplex analyses in clinical biochemistry. [Thesis]. Brno University of Technology; 2019. Available from: http://hdl.handle.net/11012/7761

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation


University of Manchester

2. Al-Sulaiman, Abdulrahman. Serological array for the diagnosis of viral infection of the central nervous system.

Degree: PhD, 2010, University of Manchester

Encephalitis caused by the alphaherpes viruses HSV 1, HSV 2 and VZV can be devastating and rapid, accurate diagnosis is required. Whilst existing molecular techniques are invaluable in diagnosing acute disease, detection of antibody is needed to confirm infection and to make a diagnosis after the acute stage or during post-infectious encephalitis. Current immunoassays are limited by the volume of sample required. The aim of this project was to develop a rapid, accurate, low sample volume assay to improve diagnosis using Luminex technology.The immunodominant proteins of HSV and VZV, glycoprotein D (gD) and glycoprotein E (gE), were expressed in insect cells using a baculovirus expression vector. Expressed proteins were purified, characterised and used to develop in-house enzyme-linked immunosorbent assays (ELISA) to detect HSV and VZV type-specific antibodies. The performance of each newly developed in-house ELISA was compared with commercial ELISA assays using well characterised serum panels. An excellent correlation between the in-house ELISAs and the commercial ELISA assays (100% for HSV gD and 99% for VZV gE) was observed. To differentiate between HSV-1 and HSV-2 a new commercial ELISA assay (Omega) utilising a branched chain peptide (peptide 55 which provides immune selection of HSV-2 specific antibody) was evaluated against two commercially available HSV-2 ELISA assays. The Omega assay showed an overall agreement of 97.6% with Western blot and other ELISA assays. The two expressed proteins, together with peptide 55, were used to develop a triplex fluorescent microbead immunoassay for the simultaneous detection and quantitation of anti-viral antibody in human sera. Initially a monoplex assay for each analyte was developed and optimised individually and then the three assays were mixed together in a triplex assay. Results for HSV-1 gD and VZV gE obtained from the triplex assay showed a 100% agreement with HSV-1 and VZV in-house ELISA results. In the case of peptide 55, the triplex assay results showed better sensitivity than the Omega ELISA assay with an overall agreement with Western blot and other assays of 98.4%. In addition, in order to facilitate the diagnosis of alphaherpesviruses CNS infections the triplex assay was joined together with a biplex fluorescent microbead immunoassay designed for detecting and measuring human IgG and albumin in CSF and serum samples. The sensitivity and reproducibility of the resultant five-analyte multiplex immunoassay and the previous triplex assays were compared and found to have equivalent sensitivity and specificity. The sensitivity and minimal sample requirements of the new assay suggests that it will be a powerful tool for the diagnosis and study of both acute and post-infectious viral encephalitis.

Subjects/Keywords: 616.9; HSV-1; HSV-2; VZV; Encephalitis; Luminex technology

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6th Edition):

Al-Sulaiman, A. (2010). Serological array for the diagnosis of viral infection of the central nervous system. (Doctoral Dissertation). University of Manchester. Retrieved from https://www.research.manchester.ac.uk/portal/en/theses/serological-array-for-the-diagnosis-of-viral-infection-of-the-central-nervous-system(5c23bcf7-0679-437a-9617-342fc53e96d7).html ; http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.529236

Chicago Manual of Style (16th Edition):

Al-Sulaiman, Abdulrahman. “Serological array for the diagnosis of viral infection of the central nervous system.” 2010. Doctoral Dissertation, University of Manchester. Accessed September 26, 2020. https://www.research.manchester.ac.uk/portal/en/theses/serological-array-for-the-diagnosis-of-viral-infection-of-the-central-nervous-system(5c23bcf7-0679-437a-9617-342fc53e96d7).html ; http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.529236.

MLA Handbook (7th Edition):

Al-Sulaiman, Abdulrahman. “Serological array for the diagnosis of viral infection of the central nervous system.” 2010. Web. 26 Sep 2020.

Vancouver:

Al-Sulaiman A. Serological array for the diagnosis of viral infection of the central nervous system. [Internet] [Doctoral dissertation]. University of Manchester; 2010. [cited 2020 Sep 26]. Available from: https://www.research.manchester.ac.uk/portal/en/theses/serological-array-for-the-diagnosis-of-viral-infection-of-the-central-nervous-system(5c23bcf7-0679-437a-9617-342fc53e96d7).html ; http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.529236.

Council of Science Editors:

Al-Sulaiman A. Serological array for the diagnosis of viral infection of the central nervous system. [Doctoral Dissertation]. University of Manchester; 2010. Available from: https://www.research.manchester.ac.uk/portal/en/theses/serological-array-for-the-diagnosis-of-viral-infection-of-the-central-nervous-system(5c23bcf7-0679-437a-9617-342fc53e96d7).html ; http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.529236

3. Al-Sulaiman, Abdulrahman. Serological Array for the Diagnosis of Viral Infection of the Central Nervous System.

Degree: 2010, University of Manchester

Encephalitis caused by the alphaherpes viruses HSV 1, HSV 2 and VZV can be devastating and rapid, accurate diagnosis is required. Whilst existing molecular techniques are invaluable in diagnosing acute disease, detection of antibody is needed to confirm infection and to make a diagnosis after the acute stage or during post-infectious encephalitis. Current immunoassays are limited by the volume of sample required. The aim of this project was to develop a rapid, accurate, low sample volume assay to improve diagnosis using Luminex technology.The immunodominant proteins of HSV and VZV, glycoprotein D (gD) and glycoprotein E (gE), were expressed in insect cells using a baculovirus expression vector. Expressed proteins were purified, characterised and used to develop in-house enzyme-linked immunosorbent assays (ELISA) to detect HSV and VZV type-specific antibodies. The performance of each newly developed in-house ELISA was compared with commercial ELISA assays using well characterised serum panels. An excellent correlation between the in-house ELISAs and the commercial ELISA assays (100% for HSV gD and 99% for VZV gE) was observed. To differentiate between HSV-1 and HSV-2 a new commercial ELISA assay (Omega) utilising a branched chain peptide (peptide 55 which provides immune selection of HSV-2 specific antibody) was evaluated against two commercially available HSV-2 ELISA assays. The Omega assay showed an overall agreement of 97.6% with Western blot and other ELISA assays. The two expressed proteins, together with peptide 55, were used to develop a triplex fluorescent microbead immunoassay for the simultaneous detection and quantitation of anti-viral antibody in human sera. Initially a monoplex assay for each analyte was developed and optimised individually and then the three assays were mixed together in a triplex assay. Results for HSV-1 gD and VZV gE obtained from the triplex assay showed a 100% agreement with HSV-1 and VZV in-house ELISA results. In the case of peptide 55, the triplex assay results showed better sensitivity than the Omega ELISA assay with an overall agreement with Western blot and other assays of 98.4%. In addition, in order to facilitate the diagnosis of alphaherpesviruses CNS infections the triplex assay was joined together with a biplex fluorescent microbead immunoassay designed for detecting and measuring human IgG and albumin in CSF and serum samples. The sensitivity and reproducibility of the resultant five-analyte multiplex immunoassay and the previous triplex assays were compared and found to have equivalent sensitivity and specificity. The sensitivity and minimal sample requirements of the new assay suggests that it will be a powerful tool for the diagnosis and study of both acute and post-infectious viral encephalitis. Advisors/Committee Members: KLAPPER, PAUL P, Vallely, Pamela, Klapper, Paul.

Subjects/Keywords: HSV-1; HSV-2; VZV; Encephalitis; Luminex technology

…volume assay to improve diagnosis using Luminex technology. The immunodominant proteins of HSV… …coded microspheres developed by Luminex ... .... 74 Figures 1.7 The method in which the… …From This Work 1. Use of Molecular Array Technology for Diagnosis and Study of Viral… …Nucleic acid Amplification Technology NCBI National Centre for Biotechnology Information ng… 

Record DetailsSimilar RecordsGoogle PlusoneFacebookTwitterCiteULikeMendeleyreddit

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6th Edition):

Al-Sulaiman, A. (2010). Serological Array for the Diagnosis of Viral Infection of the Central Nervous System. (Doctoral Dissertation). University of Manchester. Retrieved from http://www.manchester.ac.uk/escholar/uk-ac-man-scw:99539

Chicago Manual of Style (16th Edition):

Al-Sulaiman, Abdulrahman. “Serological Array for the Diagnosis of Viral Infection of the Central Nervous System.” 2010. Doctoral Dissertation, University of Manchester. Accessed September 26, 2020. http://www.manchester.ac.uk/escholar/uk-ac-man-scw:99539.

MLA Handbook (7th Edition):

Al-Sulaiman, Abdulrahman. “Serological Array for the Diagnosis of Viral Infection of the Central Nervous System.” 2010. Web. 26 Sep 2020.

Vancouver:

Al-Sulaiman A. Serological Array for the Diagnosis of Viral Infection of the Central Nervous System. [Internet] [Doctoral dissertation]. University of Manchester; 2010. [cited 2020 Sep 26]. Available from: http://www.manchester.ac.uk/escholar/uk-ac-man-scw:99539.

Council of Science Editors:

Al-Sulaiman A. Serological Array for the Diagnosis of Viral Infection of the Central Nervous System. [Doctoral Dissertation]. University of Manchester; 2010. Available from: http://www.manchester.ac.uk/escholar/uk-ac-man-scw:99539

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