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University of KwaZulu-Natal

1. [No author]. Phytochemical and elemental analysis of Cyrtanthus obliquus and Lippia javanica.

Degree: Chemistry, 2012, University of KwaZulu-Natal

A growing number of South Africans are relying more and more on alternative medicine for their healthcare needs due to the high cost of commercially available medicines and lack of medical aid. To these people, traditional medicine has provided an alternative form of treatment with medicinal benefits that are claimed to be the same as conventional medicine but at a lower cost. Many herbal tonics and concoctions are used in traditional medicine, one of which is Imbiza, a herbal tonic comprising plant parts of different medicinal plants, which is deemed to be more effective than the use of a single medicinal plant. The safety and efficacy of these herbal preparations sold in the street markets as well as in rural areas have not yet been proven. The study investigates two of the plants that are used to make Imbiza, namely Cyrtanthus obliquus bulbs and Lippia javanica leaves. Phytochemical studies of the extracts of C. obliquus bulbs yielded two new chalcones, two new dihydrochalcones and a lanostane triterpenoid. Antioxidant activity of the chalcones and dihydrochalcones was moderate and lower than ascorbic acid. GC-MS profiling of the various extracts of L. javanica leaves showed the presence of monoterpenes, sesquiterpenes and amino compounds. Total and water extractable concentrations of selected elements were determined in C. obliquus bulbs collected from eight market sites around the KwaZulu-Natal province. The levels of the elements were found to be in decreasing order of Ca > Mg > Fe > Zn > Mn > Cu ≈ Se > Pb > Cr for total concentrations and Ca > Mg > Fe > Zn > Mn for water extractable forms. A high percentage of Zn (77.5-91.5 %) was shown to extract into water. Total and water extractable concentrations of selected elements were determined in L. javanica leaves and corresponding soil samples collected from ten different locations around the KwaZulu-Natal province. The levels of the elements were found to be in decreasing order of Ca > Mg > Fe > Zn > Mn > Cu > Se > Cr > Pb > Co > Cd for total concentrations and Ca > Mg > Fe > Zn > Cu > Cr > Pb for water extractable forms. A high percentage of Cr (71.8 - 93.9 %) was shown to extract into water. Imbiza has been recognized by traditional healers and herbalist for the treatment of minor and chronic illnesses, which range from chest infections to cancer. Previous studies have shown that the compounds identified in this research (chalcones, dihydrochalcones, monoterpenes and sesquiterpenes) have indeed anticancer activities. This study therefore adds to the growing body of research on indigenous medicinal plants. Advisors/Committee Members: Jonnalagadda, S. B (advisor), Moodley, Roshila (advisor).

Subjects/Keywords: Tonics (Medicinal preparations); Cyrtanthus.; Lippia (Genus); Herbs – Therapeutic use.; Chemistry.

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6th Edition):

author], [. (2012). Phytochemical and elemental analysis of Cyrtanthus obliquus and Lippia javanica. (Thesis). University of KwaZulu-Natal. Retrieved from http://hdl.handle.net/10413/12225

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16th Edition):

author], [No. “Phytochemical and elemental analysis of Cyrtanthus obliquus and Lippia javanica. ” 2012. Thesis, University of KwaZulu-Natal. Accessed August 26, 2019. http://hdl.handle.net/10413/12225.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7th Edition):

author], [No. “Phytochemical and elemental analysis of Cyrtanthus obliquus and Lippia javanica. ” 2012. Web. 26 Aug 2019.

Vancouver:

author] [. Phytochemical and elemental analysis of Cyrtanthus obliquus and Lippia javanica. [Internet] [Thesis]. University of KwaZulu-Natal; 2012. [cited 2019 Aug 26]. Available from: http://hdl.handle.net/10413/12225.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

author] [. Phytochemical and elemental analysis of Cyrtanthus obliquus and Lippia javanica. [Thesis]. University of KwaZulu-Natal; 2012. Available from: http://hdl.handle.net/10413/12225

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

2. Ricardo de AraÃjo Marques. âEstudo QuÃmico e AvaliaÃÃo Quimiopreventiva de Humirianthera ampla Miers, Simarouba versicolor e cinco espÃcies do gÃnero Lippia.

Degree: PhD, 2011, Universidade Federal do Ceará

Humirianthera ampla Miers (Icacinaceae), Simarouba verscicolor A. St.-Hil. (Simaroubaceae), Lippia Alba (Mill) N.E. Br. (quimiotipos carvoneifera, citralifera e mirceneifera), Lippia gracillis Schauer e Lippia sidoides Cham., foram avaliadas como provÃveis fontes de compostos bioativos possuidores de atividade anticÃncer (antioxidante e/ou citotÃxica) atravÃs de ensaios in vitro, e os seus constituintes quÃmicos foram identificados e quantificados atravÃs mÃtodos espectroscÃpicos e espectromÃtricos. Em H. ampla foram identificados e quantificados cinco constituintes em suas folhas (esqualeno, α-tocoferol, campesterol, estigmasterol e β-sitosterol) e dez em suas raÃzes (Ãcido vanÃlico, Ãcido 3,4-di-hidroxibenzÃico, Ãcido sirÃngico, Ãcido gÃlico, campesterol, estigmasterol, annonalida, simiarenol, β-sitosterol e lupeol). A annonalida isolada das raÃzes de H. ampla apresentou potente atividade citotÃxica contra vÃrias linhagens de cÃlulas tumorais in vitro. Em S. versicolor foram identificados e quantificados seis constituintes em suas cascas (campesterol, estigmasterol, taraxerona, β-sitosterol, lupenona e sitostenona). A avaliaÃÃo do potencial antioxidante de H. ampla e S. versicolor apresentou resultados desapontadores quando comparados com outras espÃcies. Os Ãleos essenciais das espÃcies de Lippia foram avaliados por CG/EM e os constituintes majoritÃrios foram timol (Lippia sidoides, 65,5 %), carvona (Lippia alba carvoneifera, 48,6%) geraniol (Lippia alba citraleifera, 38,4 %; Lippia alba mircenefera, 31,9 %), nerol (Lippia alba citraleifera, 28,3 %; Lippia alba mirceneifera, 23,2 %), carvacrol (Lippia gracilis, 31,5 %) e limoneno (Lippia alba carvoneifera, 25,7 %). Os Ãleos essenciais e os extratos etanÃlicos das espÃcies de Lippia tiveram suas capacidades antioxidantes avaliadas, e somente o Ãleo essencial e o extrato etanÃlico de Lippia alba carvoneifera apresentaram resultados promissores.

Humirianthera ampla Miers (Icacinaceae), Simarouba verscicolor A. St.-Hil. (Simaroubaceae), Lippia Alba (Mill) N.E. Br. (chemotypes carvoneifera, citralifera and myrceneifera), Lippia gracillis Schauer and Lippia sidoides Cham., were evaluated as potential sources of bioactive compounds possessing anticancer activity (antioxidant or cytotoxic) by in vitro assays, and the chemical compounds were identified and quantified by epectroscopic and spectrometric methods. In H. ampla were identified and quantified five compounds in the leaves (squalene, α-tocoferol, campesterol, stigmasterol e β-sitosterol) and ten in the roots (vanillic acid, 3,4-dihydroxybenzoic acid, syringic acid, galic acid, campesterol, stigmasterol, annonalide, simiarenol, β-sitosterol and lupeol). Annonalide isolated from roots of H. ampla showed very high cytotoxicity against several cancer cell lines in vitro. In S. versicolor were identified and quantified six compounds in the bark (campesterol, stigmasterol, taraxerone, β-sitosterol, lupenone e sitostenone). The evaluation of the antioxidant capacity of H.…

Advisors/Committee Members: Maria Teresa Salles Trevisan, Gilvandete Maria Pinheiro Santiago, Janete Eliza Soares de Lima, Dennis de Oliveira Imbroisi, Lucia Maria Conserva.

Subjects/Keywords: QuimioprevenÃÃo; Lippia; Simarouba versicolor; Humirianthera ampla; QUIMICA ORGANICA; Annonalida; Humirianthera ampla; Simarouba versicolor; Lippia; Chemoprevention; Annonalide; Lippia (Genus); Simaroubaceae; Antioxidantes; Plantas medicinais - FitoquÃmica; Agentes anticÃncer

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6th Edition):

Marques, R. d. A. (2011). âEstudo QuÃmico e AvaliaÃÃo Quimiopreventiva de Humirianthera ampla Miers, Simarouba versicolor e cinco espÃcies do gÃnero Lippia.â. (Doctoral Dissertation). Universidade Federal do Ceará. Retrieved from http://www.teses.ufc.br/tde_busca/arquivo.php?codArquivo=7655 ;

Chicago Manual of Style (16th Edition):

Marques, Ricardo de AraÃjo. “âEstudo QuÃmico e AvaliaÃÃo Quimiopreventiva de Humirianthera ampla Miers, Simarouba versicolor e cinco espÃcies do gÃnero Lippia.â.” 2011. Doctoral Dissertation, Universidade Federal do Ceará. Accessed August 26, 2019. http://www.teses.ufc.br/tde_busca/arquivo.php?codArquivo=7655 ;.

MLA Handbook (7th Edition):

Marques, Ricardo de AraÃjo. “âEstudo QuÃmico e AvaliaÃÃo Quimiopreventiva de Humirianthera ampla Miers, Simarouba versicolor e cinco espÃcies do gÃnero Lippia.â.” 2011. Web. 26 Aug 2019.

Vancouver:

Marques RdA. âEstudo QuÃmico e AvaliaÃÃo Quimiopreventiva de Humirianthera ampla Miers, Simarouba versicolor e cinco espÃcies do gÃnero Lippia.â. [Internet] [Doctoral dissertation]. Universidade Federal do Ceará 2011. [cited 2019 Aug 26]. Available from: http://www.teses.ufc.br/tde_busca/arquivo.php?codArquivo=7655 ;.

Council of Science Editors:

Marques RdA. âEstudo QuÃmico e AvaliaÃÃo Quimiopreventiva de Humirianthera ampla Miers, Simarouba versicolor e cinco espÃcies do gÃnero Lippia.â. [Doctoral Dissertation]. Universidade Federal do Ceará 2011. Available from: http://www.teses.ufc.br/tde_busca/arquivo.php?codArquivo=7655 ;

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