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You searched for subject:(Linamarin). Showing records 1 – 2 of 2 total matches.

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NSYSU

1. Liu, Cheng-Yu. Colorimetric Assay for Cyanide and Application in Monitored Cyanogenic Glycoside Using Polysorbate 40-Stabilized Gold Nanoparticles.

Degree: Master, Chemistry, 2011, NSYSU

1. Colorimetric Assay for Cyanide and Cyanogenic Glycoside Using Polysorbate 40-Stabilized Gold Nanoparticles. This study described a simple and homogeneous method for the selective and sensitive detection of cyanide and endogenous biological cyanide using polysorbate 40-stabilized gold nanoparticles (PS 40-AuNPs). Neutral PS 40 molecules enable citrate-capped AuNPs to stabilize in a high-salinity solution. The addition of cyanide to a solution of PS 40-AuNPs resulted in the formation of AuCN(s) on the NP surface and Au(CN)2â in an aqueous solution. The removal of PS 40 molecules from the NP surface rendered the AuNPs unstable in a high-salinity solution, leading to NP aggregation. The formation of AuCN(s) and Au(CN)2â was demonstrated by means of surface-assisted laser desorption/ionization time of flight mass spectrometry and inductively coupled plasma mass spectroscopy, respectively. PS 40-AuNPs were capable of selectively detecting cyanide at concentrations as low as 500 nM. Additionally, the minimum detectable concentration of linamarin (cyanogenic glycoside) was measured to be 1 uM using PS 40-AuNPs. This probe was successfully applied to the determination of cyanide in tap water, the monitor of cyanide removal during food processing, and the quantification of linamarin in cassava root. 2. Colorimetric detoxification and monitored cyanogenic glycoside in plants/fruit using polysorbate 40-stabilized gold nanoparticles. Developing rapid, highly sensitive, and selective detection/inhibition of cyanide/cyanogenic glycoside from plants and foods is extremely essential for human life safety. Here we report a strategy for the colormetric visualization of cyanogenic glycoside using polysorbate 40 stabilized gold nanoparticle (PS 40-AuNPs). Two cyanogenic glycosides (amygdalin and linamarin) were chosen to determine the efficiency of acid hydrolysis. According to US Department and Health and Human Services standard cyanide antidote kit, sodium thiosulfate and hydroxocobalamin (vitamin B12a) seems to be an appropriate antidote for treatment cyanide poisoning victims. The addition of thiosulfate/vitamin B12a to a solution of cyanide/cyanogenic glycosides resulted in the formation of thiocyanate/vitamin B12 in an aqueous solution, which couldnât etch PS 40-AuNPs and inhibit the aggregation of PS 40-AuNPs in a high-salt solution. The inhibition/detoxification efficiency (IC50) of thiosulfate and vitamin B12a were studied for treatment of cyanide and hydrolyzed cyanogenic glycoside. This probe was also used to monitor the removal of cyanide, estimated the concentration of cyanide and detoxification of cyanide by thiosulfate in plants/fruit sample. Advisors/Committee Members: Chao-Ming Chiang (chair), Shiuh-Jen Jiang (chair), Wei-Lung Tseng (committee member).

Subjects/Keywords: Linamarin; Amygdalin; Cyanogenic glycoside; Cyanide; AuNPs

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APA (6th Edition):

Liu, C. (2011). Colorimetric Assay for Cyanide and Application in Monitored Cyanogenic Glycoside Using Polysorbate 40-Stabilized Gold Nanoparticles. (Thesis). NSYSU. Retrieved from http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0825111-121912

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16th Edition):

Liu, Cheng-Yu. “Colorimetric Assay for Cyanide and Application in Monitored Cyanogenic Glycoside Using Polysorbate 40-Stabilized Gold Nanoparticles.” 2011. Thesis, NSYSU. Accessed December 05, 2019. http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0825111-121912.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7th Edition):

Liu, Cheng-Yu. “Colorimetric Assay for Cyanide and Application in Monitored Cyanogenic Glycoside Using Polysorbate 40-Stabilized Gold Nanoparticles.” 2011. Web. 05 Dec 2019.

Vancouver:

Liu C. Colorimetric Assay for Cyanide and Application in Monitored Cyanogenic Glycoside Using Polysorbate 40-Stabilized Gold Nanoparticles. [Internet] [Thesis]. NSYSU; 2011. [cited 2019 Dec 05]. Available from: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0825111-121912.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Liu C. Colorimetric Assay for Cyanide and Application in Monitored Cyanogenic Glycoside Using Polysorbate 40-Stabilized Gold Nanoparticles. [Thesis]. NSYSU; 2011. Available from: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0825111-121912

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation


The Ohio State University

2. Leyva-Guerrero, Elisa. Enhancement of the free amino acid and protein content of cassava storage roots and evaluation of root-specific promoters in cassava.

Degree: PhD, Plant Cellular and Molecular Biology, 2011, The Ohio State University

Cassava is an important staple crop for millions of people around the world in particular in Sub-Saharan Africa. Cassava storage roots are a good source of calories, however, they are deficient in protein and other micronutrients such as iron and zinc. Protein malnutrition is widespread in the regions were cassava is widely consumed and consumption of cassava as a staple has been linked to reduced protein intake in the diet. A cassava storage root with higher protein could potentially impact the nutrition and well being of millions people.In this thesis we propose two approaches for increasing free amino acids and protein in cassava storage roots. One approach was to increase nitrate assimilation through the expression of a mutated nitrate reductase. Nitrate reduction to nitrite is followed by a further reduction to ammonium which can be readily incorporated into amino acids. A rate limiting step in nitrate metabolism is the reduction of nitrate to nitrite by nitrate reductase. This enzyme is highly regulated, however, it has been observed that through mutation of a key regulatory serine the enzyme remains active and the assimilation of nitrate increases resulting in increased free amino acid levels. In cassava the root-specific expression of a mutated nitrate reductase resulted in a doubling of the storage root free amino acid content; however, no root protein increase was observed. A second approach was to utilize the nitrile group present in the cassava cyanogenic glucoside linamarin as a reduced nitrogen source. The hydrolysis of linamarin by the enzyme linamarase releases acetone cyanohydrin which in turn can degrade to release cyanide. In all plants there is a cyanide assimilation pathway involving ß-cyanoalanine synthase, the end products of this pathway are aspartate and ammonia. Through increased hydrolysis of linamarin, we sought to increase the assimilation of cyanide in to aspartate and ammonia. In wild-type cassava the interaction between linamarase and linamarin is limited spatially; linamarase is found in the cell wall and linamarin in the vacuole. Through the expression of a vacuolar targeted linamarase, we proposed to increase the hydrolysis of linamarin and as a result provide more reduced nitrogen (nitrile) for free amino acid and protein synthesis. The expression of a vacuolar linamarase in cassava storage roots resulted in doubling of the free amino acid pool in this organ but no increase in protein. It was only through the dual expression of vacuolar linamarase and the storage protein sporazein that both an increase in free amino acid and protein in the storage roots was observed. Although cassava is a main staple food the molecular biology tools available for its transformation are very limited. In the final chapter of this thesis we evaluated four different Arabidopsis root promoters in cassava to determine their functionality and tissue specificity. Two promoters named A14 and E40 were determined to be functional in cassava roots with minimal leaf expression. The availability of an increased… Advisors/Committee Members: Sayre, Richard (Advisor), Hamel, Patrice (Advisor).

Subjects/Keywords: Botany; Plant Biology; cassava; free amino acid increase; protein increase; cyanogenic glucosides; linamarin; promoters; nitrate reductase; linamarase; sporazein; storage root

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6th Edition):

Leyva-Guerrero, E. (2011). Enhancement of the free amino acid and protein content of cassava storage roots and evaluation of root-specific promoters in cassava. (Doctoral Dissertation). The Ohio State University. Retrieved from http://rave.ohiolink.edu/etdc/view?acc_num=osu1299535104

Chicago Manual of Style (16th Edition):

Leyva-Guerrero, Elisa. “Enhancement of the free amino acid and protein content of cassava storage roots and evaluation of root-specific promoters in cassava.” 2011. Doctoral Dissertation, The Ohio State University. Accessed December 05, 2019. http://rave.ohiolink.edu/etdc/view?acc_num=osu1299535104.

MLA Handbook (7th Edition):

Leyva-Guerrero, Elisa. “Enhancement of the free amino acid and protein content of cassava storage roots and evaluation of root-specific promoters in cassava.” 2011. Web. 05 Dec 2019.

Vancouver:

Leyva-Guerrero E. Enhancement of the free amino acid and protein content of cassava storage roots and evaluation of root-specific promoters in cassava. [Internet] [Doctoral dissertation]. The Ohio State University; 2011. [cited 2019 Dec 05]. Available from: http://rave.ohiolink.edu/etdc/view?acc_num=osu1299535104.

Council of Science Editors:

Leyva-Guerrero E. Enhancement of the free amino acid and protein content of cassava storage roots and evaluation of root-specific promoters in cassava. [Doctoral Dissertation]. The Ohio State University; 2011. Available from: http://rave.ohiolink.edu/etdc/view?acc_num=osu1299535104

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