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Addis Ababa University
1.
Dawit, Tewolde.
Evaluation of some wood rotting fungi for bagasse pretreatment in ethanol production
.
Degree: 2012, Addis Ababa University
URL: http://etd.aau.edu.et/dspace/handle/123456789/371
► Lignocellulosic biomass is a sustainable feedstock for ethanol production compared to other substrates. In the ethanol generation process, pretreatment makes cellulose more accessible to enzymatic…
(more)
▼ Lignocellulosic biomass is a sustainable feedstock for ethanol production compared to other
substrates. In the ethanol generation process, pretreatment makes cellulose more accessible to
enzymatic hydrolysis by modification of the lignin barrier. Biological pretreatment employs
selective rot fungi that preferentially degrade lignin with minimum loss of polysaccharides.
The system is not well developed; but is advantageous over other pretreatments because
reactions are enzymatic. These fungi produce
ligninolytic enzymes, predominantly lignin
peroxidase (LiP), manganese peroxidase (MnP) and laccase (Lac) in different combinations.
Studies on biological pretreatments have shown lignin weight loss and improvement in
ethanol yield. However, only few well characterised white rots have been explored. The aim
of this study was to evaluate the potential of 15 wood rotting fungi isolated from Ethiopia for
pretreatment during 15 days solid state fermentation, using bagasse as lignocellulosic
substrate. The production of
ligninolytic enzymes by Fomitiporia aethiopica, F.
pseudopunctata, Fomitopsis carnea and Vanderbylia vicina were reported for the first time in
this study. The white rots, F. aethiopica, Perenniporia tephropora, Inonotus sp. and
Pleurotus sajor-caju, were identified as relatively highly selective based on maximum and
minimum productivity of
ligninolytic and polysaccharide degrading
enzymes, respectively.
The pretreatment by the white rots caused ligninolysis and better cellulose digestibility was
obtained with higher lignin loss. Among the selective degraders, P. tephropora caused the
highest lignin loss (7.71%) and cellulose digestibility (29.44 %) after enzyme hydrolysis of
the pretreated bagasse. This digestibility showed an improvement by 38.74 % in comparison
with untreated bagasse. In addition to high MnP productivity (55.87 U/g), P. tephropora also
produced high titers of Lac (79.65 U/g) in contrast to the other selective degraders that might
have attributed to better lignin loss. The ethanol yield from fermentation of cellulase enzyme
hydrolysed P. tephropora pretreated bagasse was 1.87 g/L, which was an improvement by
27.21 % compared with untreated bagasse (1.47 g/L). Therefore, P. tephropora pretreatment
enhances ethanol production from bagasse through partial degradation of lignin, which
improves the accessibility of cellulose to enzyme hydrolysis.
Advisors/Committee Members: Amare Gessesse (Ph.D) (advisor).
Subjects/Keywords: bagasse;
ethanol;
ligninolytic enzymes;
pretreatment
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APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
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APA (6th Edition):
Dawit, T. (2012). Evaluation of some wood rotting fungi for bagasse pretreatment in ethanol production
. (Thesis). Addis Ababa University. Retrieved from http://etd.aau.edu.et/dspace/handle/123456789/371
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Dawit, Tewolde. “Evaluation of some wood rotting fungi for bagasse pretreatment in ethanol production
.” 2012. Thesis, Addis Ababa University. Accessed January 16, 2021.
http://etd.aau.edu.et/dspace/handle/123456789/371.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Dawit, Tewolde. “Evaluation of some wood rotting fungi for bagasse pretreatment in ethanol production
.” 2012. Web. 16 Jan 2021.
Vancouver:
Dawit T. Evaluation of some wood rotting fungi for bagasse pretreatment in ethanol production
. [Internet] [Thesis]. Addis Ababa University; 2012. [cited 2021 Jan 16].
Available from: http://etd.aau.edu.et/dspace/handle/123456789/371.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Dawit T. Evaluation of some wood rotting fungi for bagasse pretreatment in ethanol production
. [Thesis]. Addis Ababa University; 2012. Available from: http://etd.aau.edu.et/dspace/handle/123456789/371
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Univerzitet u Beogradu
2.
Knežević, Aleksandar Z., 1981-.
Ligninolitički potencijal i medicinska svojstva
ekstrakata vrsta roda Trametes fr.
Degree: Biološki fakultet, 2016, Univerzitet u Beogradu
URL: https://fedorabg.bg.ac.rs/fedora/get/o:10415/bdef:Content/get
► Biologija - Algologija i mikologija / Biology - Algology and Mycology
Rod Trametes Fr. obuhvata vrste širokog rasprostranjena sa dobro razvijenim ligninolitičkim enzimskim sistemom i…
(more)
▼ Biologija - Algologija i mikologija / Biology -
Algology and Mycology
Rod Trametes Fr. obuhvata vrste širokog
rasprostranjena sa dobro razvijenim ligninolitičkim enzimskim
sistemom i značajnim kapacitetom u sintezi raznovrsnih biološki
aktivnih jedinjenja, zbog čega sve više nalaze primenu u
mnogobrojnim biotehnološkim procesima. Ciljevi ovog istraživanja
bili su optimizacija uslova kultivacije za produkciju
ligninolitičkih enzima i degradaciju odabranog biljnog otpada sa T.
gibbosa, praćenje varijabilnosti u kapacitetu lignocelulolize
unutar roda Trametes kao i određivanje antifungalnog,
antioksidativnog, genoprotektivnog, citotoksičnog i
anti-neurodegenerativnog efekta ekstrakata plodonosnih tela i
micelije. Pšenična slama je bila bolji izvor ugljenika nego
piljevina bukve za produkciju Mn-oksidujućih peroksidaza i lakaza
T. gibbosa koji je maksimalni stepen delignifikacije (48,3%)
postigao nakon 19 dana čvrste fermentacije u prisustvu 10,0 mM
azota u formi (NH4)2SO4 i 10% pšeničnih mekinja kao dodatnog izvora
azota. Prisustvo Fe2+ i Zn2+ u koncentraciji od 0,5 mM, kao i 0,1
mM p-anizidina i 0,5% veratril alkohola je značajno stimulisalo
ligninolizu. Značajna inter- i intra-specijska varijabilnost u
sintezi proučavanih enzima, degradaciji polimera biljnog ćelijskog
zida kao i u selektivnosti delignifikacije je takođe uočena između
6 vrsta i 11 sojeva. T. hirsuta BEOFB 30 je bio najefikasniji i
najselektivniji razlagač lignina (56,0% razloženog lignina prema
17,4% hidrolizovane celuloze), posebno u prisustvu p-anizidina koji
je stimulisao produkciju Mn-oksidujućih peroksidaza. Dvofaktorskom
analizom varijanse je pokazano da su soj, prisustvo indukujućih
supstanci kao i njihova interakcija faktori koji značajno utiču na
stepen ligninolize. Etanolni ekstrakti bazidiokarpa i micelije T.
gibbosa, T. hirsuta i T. versicolor, su imali slab antifungalni
potencijal koji se ogledao u slaboj inhibiciji rasta testiranih
humanih patogena a jedino je micelijski ekstrakt T. hirsuta pokazao
fungicidno dejstvo na Aspergillus glaucus (MFC = 32,0 mg/mL).
Ekstrakt plodonosnih tela T. versicolor je pokazao najveći
kapacitet neutralizacije DPPH i ABTS radikala (dobijene EC50
vrednosti su 15,22 odnosno 8,46 mg/mL), a glavni nosioci ove
aktivnosti su bili fenoli čija je koncentracija bila u pozitivnoj
korelaciji sa antioksidativnim kapacitetom...
Advisors/Committee Members: Stajić, Mirjana, 1970-.
Subjects/Keywords: ligninolytic enzymes; biodegradation; biological
activity
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❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Knežević, Aleksandar Z., 1. (2016). Ligninolitički potencijal i medicinska svojstva
ekstrakata vrsta roda Trametes fr. (Thesis). Univerzitet u Beogradu. Retrieved from https://fedorabg.bg.ac.rs/fedora/get/o:10415/bdef:Content/get
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Knežević, Aleksandar Z., 1981-. “Ligninolitički potencijal i medicinska svojstva
ekstrakata vrsta roda Trametes fr.” 2016. Thesis, Univerzitet u Beogradu. Accessed January 16, 2021.
https://fedorabg.bg.ac.rs/fedora/get/o:10415/bdef:Content/get.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Knežević, Aleksandar Z., 1981-. “Ligninolitički potencijal i medicinska svojstva
ekstrakata vrsta roda Trametes fr.” 2016. Web. 16 Jan 2021.
Vancouver:
Knežević, Aleksandar Z. 1. Ligninolitički potencijal i medicinska svojstva
ekstrakata vrsta roda Trametes fr. [Internet] [Thesis]. Univerzitet u Beogradu; 2016. [cited 2021 Jan 16].
Available from: https://fedorabg.bg.ac.rs/fedora/get/o:10415/bdef:Content/get.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Knežević, Aleksandar Z. 1. Ligninolitički potencijal i medicinska svojstva
ekstrakata vrsta roda Trametes fr. [Thesis]. Univerzitet u Beogradu; 2016. Available from: https://fedorabg.bg.ac.rs/fedora/get/o:10415/bdef:Content/get
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of South Africa
3.
Ijoma, Grace Nkechinyere.
Enzyme production and activities of lignocellulolytic fungi cultivated on agricultural residues
.
Degree: 2016, University of South Africa
URL: http://hdl.handle.net/10500/22962
► A total of 30 fungal fruiting bodies were collected from decaying plant materials (barks and litter) from the wild based on morphological variations. Nine of…
(more)
▼ A total of 30 fungal fruiting bodies were collected from decaying plant materials (barks and litter) from the wild based on morphological variations. Nine of these fungi purified to monoaxenic cultures were included in the present study and also a type strain Ganoderma lucidum ATCC- 32471. These fungi were screened for lignocellulolytic activities, five of these organisms produced
ligninolytic enzymes when exposed to two different concentrations of guaiacol (0.02% and 0.2%) on two different media (MEA and PDA). All ten fungal isolates screened for cellulolytic activity were positive for the production of the cellulase enzyme. The fungal isolates were characterised using morphological and molecular methods. Molecular characterization using ITS1 and ITS4 primers was able to identify these fungal isolates to degrees of accuracy ranging from 98% to 100%. The phylogenetic and lineage analysis showed that the species varied amongst phylum Basidiomycota, Ascomycota and early diverging fungal lineages Mucormycotina. Both monocultures and dual cultures of these 10 fungal species were cultivated for the purpose of spectrophotometrically quantifying and evaluating enzyme production on agricultural waste residues; corn cob, sugar cane bagasse and wheat straw.
A pattern of antagonistic invasion interaction was identified to demonstrate increased enzyme production on dual cultures. Four of these fungal species, Trichoderma sp. KN10, Rhizopus microsporus KN2, Fomitopsis sp. KN1 and Coriolopsis sp. KN6 demonstrated tendencies of invasion and replacement in co-cultures. The fungi and their dual cultures showed varying levels of enzyme production. Analysis of mean showed dual culture interactions involving KN10 with values for MnP production approximately at 1.46U/ml compared to monoculture of 0.06U/ml. Further, dual laccase values approximately at 0.09U/ml compared to monocultures of 0.05U/ml. Overall the highest enzyme activity was observed using wheat straw. This study demonstrated and proved that agricultural waste residues can be used for lignocellulytic enzyme production and that antagonistic invasion by some fungi (in particular Trichoderma sp. KN10) in co-cultures can increase production of one or more of the three enzyme laccase, lignin peroxidase and manganese peroxidase.
Advisors/Committee Members: Tekere, M (advisor).
Subjects/Keywords: Fungi;
Dual cultures;
Competition;
Antagonism;
Ligninolytic enzymes;
Agricultural residues
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❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Ijoma, G. N. (2016). Enzyme production and activities of lignocellulolytic fungi cultivated on agricultural residues
. (Doctoral Dissertation). University of South Africa. Retrieved from http://hdl.handle.net/10500/22962
Chicago Manual of Style (16th Edition):
Ijoma, Grace Nkechinyere. “Enzyme production and activities of lignocellulolytic fungi cultivated on agricultural residues
.” 2016. Doctoral Dissertation, University of South Africa. Accessed January 16, 2021.
http://hdl.handle.net/10500/22962.
MLA Handbook (7th Edition):
Ijoma, Grace Nkechinyere. “Enzyme production and activities of lignocellulolytic fungi cultivated on agricultural residues
.” 2016. Web. 16 Jan 2021.
Vancouver:
Ijoma GN. Enzyme production and activities of lignocellulolytic fungi cultivated on agricultural residues
. [Internet] [Doctoral dissertation]. University of South Africa; 2016. [cited 2021 Jan 16].
Available from: http://hdl.handle.net/10500/22962.
Council of Science Editors:
Ijoma GN. Enzyme production and activities of lignocellulolytic fungi cultivated on agricultural residues
. [Doctoral Dissertation]. University of South Africa; 2016. Available from: http://hdl.handle.net/10500/22962
4.
Sartori, Sergio Birello.
Atividade enzimática e valores nutricionais de Pleurotus spp. cultivados em vinhaça.
Degree: Mestrado, Microbiologia Agrícola, 2012, University of São Paulo
URL: http://www.teses.usp.br/teses/disponiveis/11/11138/tde-21032012-100458/
;
► A vinhaça é o principal subproduto na fabricação do álcool e o que possui maior quantidade de nutrientes em sua composição. Seu poder poluente, cerca…
(more)
▼ A vinhaça é o principal subproduto na fabricação do álcool e o que possui maior quantidade de nutrientes em sua composição. Seu poder poluente, cerca de cem vezes maior que esgoto doméstico, é decorrente da sua riqueza em matéria orgânica, baixo pH, corrosividade, elevadas DBO e DQO, além de cor escura e cheiro característico. A aceitação crescente no uso de fungos basidiomicetos em processos de tratamento e/ou biorremediação se deve por apresentarem um sistema enzimático extracelular capaz de romper uma grande quantidade de ligações químicas. Espécies do gênero Pleurotus sp. são conhecidas pela produção de enzimas lignolíticas como manganês-peroxidase, peroxidases e lacases. Também são reportados como alimentos por possuírem como seus maiores constituintes carboidratos e proteínas, seguidos de minerais e baixos teores de gorduras. O presente estudo teve por objetivo avaliar a atividade enzimática de quatro fungos basidiomicetos cultivados em meio de vinhaça líquido e sólido, bem como avaliar as propriedades nutricionais dos micélios como fonte de alimento para peixes da espécie D. rerio. Os fungos Pleurotus sajor-caju CCB 020, Pleurotus sp. CCB 068, P. flabellatus CCB 396 e Pleurotus shimeji, foram cultivados em meio sólido e líquido de vinhaça para análise de atividade enzimática e para crescimento micelial durante 15 dias. A atividade enzimática foi determinada de acordo com protocolo de Szklars et al. (1989) e Kuwahara et al. (1984) a cada 3 dias. Os fungos foram liofilizados e oferecidos aos peixes na proporção de 2% do peso corpóreo dos mesmos e nas quantidades de 50% fungo + 50% ração e 100% fungo em cinco réplicas comparando com um grupo controle tratado com 100% ração por um período de 28 dias. As quatro espécies de Pleurotus apresentaram crescimento satisfatório em meio contendo 100% de vinhaça após 15 dias, sendo obtidos maiores valores com Pleurotus sp. CCB 068 (16,27g). Além disso, observou-se o clareamento e diminuição de turbidez da vinhaça pelos fungos P. sajor-caju CCB 020 e P. shimeji. Em relação à atividade enzimática, os maiores valores para lacase foram obtidos em meio de vinhaça + ágar pelo fungo P. sajor-caju (1.247, 43 UI g-1MS -12º dia) e para peroxidase apresentou, ao 6º dia, atividade de 676,02 UI g-1MS. O mesmo fungo obteve maior valor de MnP no 15º dia de cultivo (318,94 UI L-1) em meio de vinhaça líquido. As análises bromatológicas apresentaram valores nutritivos satisfatórios para os fungos quando comparados à ração comercial, apresentando menores valores somente para proteínas. O teste de alimentação com D. rerio durante 28 dia mostrou que houve perda de biomassa, não significativa estatisticamente e, os valores de sobrevivência dos peixes foram de 100% em todos os tratamentos, indicando que os fungos testados cultivados em vinhaça não apresentaram toxidade. Foi concluído que o uso de fungos do gênero Pleurotus sp. cultivados em vinhaça, além de reduzir compostos tóxicos deste subproduto, é eficiente na produção de enzimas de interesse industrial e seus micélios podem ser inclusos na…
Advisors/Committee Members: Monteiro, Regina Teresa Rosim.
Subjects/Keywords: Enzimas lignolíticas; Fungos - Propriedades nutricionais; ligninolytic enzymes; nutritional properties; Peixes; Pleurotus sp.; vinasse; Vinhaça
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❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Sartori, S. B. (2012). Atividade enzimática e valores nutricionais de Pleurotus spp. cultivados em vinhaça. (Masters Thesis). University of São Paulo. Retrieved from http://www.teses.usp.br/teses/disponiveis/11/11138/tde-21032012-100458/ ;
Chicago Manual of Style (16th Edition):
Sartori, Sergio Birello. “Atividade enzimática e valores nutricionais de Pleurotus spp. cultivados em vinhaça.” 2012. Masters Thesis, University of São Paulo. Accessed January 16, 2021.
http://www.teses.usp.br/teses/disponiveis/11/11138/tde-21032012-100458/ ;.
MLA Handbook (7th Edition):
Sartori, Sergio Birello. “Atividade enzimática e valores nutricionais de Pleurotus spp. cultivados em vinhaça.” 2012. Web. 16 Jan 2021.
Vancouver:
Sartori SB. Atividade enzimática e valores nutricionais de Pleurotus spp. cultivados em vinhaça. [Internet] [Masters thesis]. University of São Paulo; 2012. [cited 2021 Jan 16].
Available from: http://www.teses.usp.br/teses/disponiveis/11/11138/tde-21032012-100458/ ;.
Council of Science Editors:
Sartori SB. Atividade enzimática e valores nutricionais de Pleurotus spp. cultivados em vinhaça. [Masters Thesis]. University of São Paulo; 2012. Available from: http://www.teses.usp.br/teses/disponiveis/11/11138/tde-21032012-100458/ ;
5.
Zerva, Anastasia.
Βιοτεχνολογική αξιοποίηση μικροβιακού και ενζυμικού συστήματος λιγνινολυτικών μυκήτων.
Degree: 2017, National Technical University of Athens (NTUA); Εθνικό Μετσόβιο Πολυτεχνείο (ΕΜΠ)
URL: http://hdl.handle.net/10442/hedi/40556
► The main aim of the present doctoral thesis was the investigation of the biotechnological potential of the ligninolytic fungi, at the whole-cell level as well…
(more)
▼ The main aim of the present doctoral thesis was the investigation of the biotechnological potential of the ligninolytic fungi, at the whole-cell level as well as at the level of the enzymatic systems responsible for the oxidative degradation of lignocellulosic biomass. Towards that goal, in the first steps of the thesis, two basidiomycetes strains were tested regarding their ability to degrade waste materials of high phenolic content. The waste material chosen for this purpose was the olive oil mill wastewater (OMWW) a product that poses serious problems for olive-oil producing countries due to its high organic load and its high concentration of phenol compounds. The basidiomycetes strains Pleurotus citrinopileatus LGAM 28684 and Irpex lacteus LGAM 238 were tested regarding their ability to degrade OMWW, while the effect of several parameters, such as the pH and the nature and concentration of the nitrogen supplementation, in the degradation capacity was also investigated. The optimum conditions for each strain were found to be pH 6, 30 g L-1 corn steep liquor for P. citrinopileatus and 20 g L-1 for I. lacteus. The two strains showed very satisfactory levels of OMWW degradation and extracellular ligninolytic enzymes production, achieving over 90% phenols reduction and decolourization. In the next step, the ability of intracellular and extracellular polysaccharides production from three basidiomycetes strains was tested in submerged culture, and the simultaneous degradation and valorization of the OMWW was attempted through the production of fungal polysaccharides. The basidiomycetes strains selected for this purpose were Ganoderma lucidum LGAM 9720, Pleurotus citrinopileatus LGAM 28684 and Pleurotus ostreatus LGAM 1123, and they were grown in two different media for comparison, a semi-synthetic medium with xylose as carbon source and an OMWW-based medium, while two different methods were tested for the isolation of the biomass polysaccharides. The highest levels of OMWW degradation were observed from G. lucidum, while the extracellular polysaccharides isolated from all strains were found to be richer in glucan content in the case of semi-synthetic medium growth compared to OMWW-growth. Regarding the intracellular polysaccharides, the P. ostreatus biomass was found to be richer in glucans, with total glucan content 8.68% (w/w) during growth in semi-synthetic medium and 7.58% (w/w) during growth in OMWW- based medium. After application of the two isolation methods in the mycelial biomass, the fraction with the highest glucan content was found to be the one from G. lucidum after growth in semi-synthetic medium, containing 49.1% (w/w) total glucans. The FTIR spectra of the isolated fractions revealed the existence of α- and β- glucosidic bonds, but also the existence of protein contamination, indicating that the removal of α-glucans and proteins was not entirely successful with neither of the two tested methods. However, the results of the present thesis indicate that the simultaneous degradation and valorization of OMWW…
Subjects/Keywords: Βασιδιομύκητες; Γλυκάνες; Λιγνινολυτικά ένζυμα; Βιοκατάλυση; Φαινολικά πολυμερή; Basidiomycetes; Glucans; Ligninolytic enzymes; Biocatalysis; Phenol polymers
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❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Zerva, A. (2017). Βιοτεχνολογική αξιοποίηση μικροβιακού και ενζυμικού συστήματος λιγνινολυτικών μυκήτων. (Thesis). National Technical University of Athens (NTUA); Εθνικό Μετσόβιο Πολυτεχνείο (ΕΜΠ). Retrieved from http://hdl.handle.net/10442/hedi/40556
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Zerva, Anastasia. “Βιοτεχνολογική αξιοποίηση μικροβιακού και ενζυμικού συστήματος λιγνινολυτικών μυκήτων.” 2017. Thesis, National Technical University of Athens (NTUA); Εθνικό Μετσόβιο Πολυτεχνείο (ΕΜΠ). Accessed January 16, 2021.
http://hdl.handle.net/10442/hedi/40556.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Zerva, Anastasia. “Βιοτεχνολογική αξιοποίηση μικροβιακού και ενζυμικού συστήματος λιγνινολυτικών μυκήτων.” 2017. Web. 16 Jan 2021.
Vancouver:
Zerva A. Βιοτεχνολογική αξιοποίηση μικροβιακού και ενζυμικού συστήματος λιγνινολυτικών μυκήτων. [Internet] [Thesis]. National Technical University of Athens (NTUA); Εθνικό Μετσόβιο Πολυτεχνείο (ΕΜΠ); 2017. [cited 2021 Jan 16].
Available from: http://hdl.handle.net/10442/hedi/40556.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Zerva A. Βιοτεχνολογική αξιοποίηση μικροβιακού και ενζυμικού συστήματος λιγνινολυτικών μυκήτων. [Thesis]. National Technical University of Athens (NTUA); Εθνικό Μετσόβιο Πολυτεχνείο (ΕΜΠ); 2017. Available from: http://hdl.handle.net/10442/hedi/40556
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
6.
Shamala,Ahammed.
Production and Characterization of Lignin Peroxidases from Mangrove Ascomycetes.
Degree: 2002, Cochin University of Science and Technology
URL: http://dyuthi.cusat.ac.in/purl/24
► In this thesis, the production and characterization of ligninolytic enzymes using the fungi isolated from mangrove area are studied. The objective of the present work…
(more)
▼ In this thesis, the production and characterization of ligninolytic enzymes using the fungi isolated from mangrove area are studied. The objective of the present work are isolation and screening of dye decolorizing micro-organisms from mangrove area, screening of the selected microorganisms for the production of lignin degrading enzymes, identification of the potent micro-organisms, characterization of the crude enzyme, lignin peroxidase, of the selected fungi—Aspergillus sp. SIP 11 and Penicillium sp. SIP 10 etc. This included the determination of the optimum pH, temperature, veratryl alcohol and H2O2 concentration. Besides the stability of crude LiP at different pHs and temperatures were studied. The immense applications, particularly in bioremediation, to which the lignin degrading micro-organisms could be used make this study important, the ascomycetes and deuteromycetes fungi, especially form the marine environment were studied with respect to their ligninolytic enzyme system making this study an initial step in unraveling the vast hidden potential of these microbes in bioremediation, the marine microbes are halophilic in nature which make them better suited to cope with the high salinity of industrial effluents thereby giving them added advantage in the filed of bioremediation. The thesis deals with the isolation and screening of lignin degrading enzyme-producing microbes from mangrove area. The identification of the most potent fungal isolates and characterization of LiP from these are also done.
Subjects/Keywords: Lignin Peroxidases; Mangrove Ascomycetes; ligninolytic enzymes; Aspergillus
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APA ·
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APA (6th Edition):
Shamala,Ahammed. (2002). Production and Characterization of Lignin Peroxidases from Mangrove Ascomycetes. (Thesis). Cochin University of Science and Technology. Retrieved from http://dyuthi.cusat.ac.in/purl/24
Note: this citation may be lacking information needed for this citation format:
Author name may be incomplete
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Shamala,Ahammed. “Production and Characterization of Lignin Peroxidases from Mangrove Ascomycetes.” 2002. Thesis, Cochin University of Science and Technology. Accessed January 16, 2021.
http://dyuthi.cusat.ac.in/purl/24.
Note: this citation may be lacking information needed for this citation format:
Author name may be incomplete
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Shamala,Ahammed. “Production and Characterization of Lignin Peroxidases from Mangrove Ascomycetes.” 2002. Web. 16 Jan 2021.
Note: this citation may be lacking information needed for this citation format:
Author name may be incomplete
Vancouver:
Shamala,Ahammed. Production and Characterization of Lignin Peroxidases from Mangrove Ascomycetes. [Internet] [Thesis]. Cochin University of Science and Technology; 2002. [cited 2021 Jan 16].
Available from: http://dyuthi.cusat.ac.in/purl/24.
Note: this citation may be lacking information needed for this citation format:
Author name may be incomplete
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Shamala,Ahammed. Production and Characterization of Lignin Peroxidases from Mangrove Ascomycetes. [Thesis]. Cochin University of Science and Technology; 2002. Available from: http://dyuthi.cusat.ac.in/purl/24
Note: this citation may be lacking information needed for this citation format:
Author name may be incomplete
Not specified: Masters Thesis or Doctoral Dissertation
7.
Fasanella, Cristiane Cipola.
Ação das enzimas ligninolíticas produzidas por Aspergillus niger e Penicillium sp. em bagaço de cana-de-açúcar tratado quimicamente.
Degree: Mestrado, Microbiologia Agrícola, 2009, University of São Paulo
URL: http://www.teses.usp.br/teses/disponiveis/11/11138/tde-10022009-082433/
;
► A cana-de-açúcar é uma das matérias primas para a produção de açúcar e álcool. Durante o processo de produção, é gerado como subproduto (ou resíduo)…
(more)
▼ A cana-de-açúcar é uma das matérias primas para a produção de açúcar e álcool. Durante o processo de produção, é gerado como subproduto (ou resíduo) o bagaço, que possui várias aplicações, entre elas a geração de energia, fertilizantes, produção de combustíveis e ração animal. O bagaço da cana-de-açúcar é composto principalmente por materiais lignocelulósicos, possuindo como constituintes principais a celulose, a hemicelulose e a lignina. A lignina é um dos materiais mais recalcitrantes na natureza e, conseqüentemente, dificulta o acesso de enzimas aos carboidratos fermentáveis, reduzindo a eficiência da degradação da celulose e da fermentação. Uma das vias de degradação da lignina ocorre através da ação de enzimas produzidas por fungos de degradação branca, marrom e macia. Essas enzimas são capazes de degradar e expor a celulose e a hemicelulose, que, por sua vez são prontamente utilizadas por outros microrganismos. Para que isso ocorra, esses fungos promovem um processo de oxidação de compostos fenólicos e não fenólicos da molécula de lignina por meio de enzimas ligninolíticas extracelulares entre elas a lacase e a manganês peroxidase (MnP), em baixa velocidade, porém com grande eficiência. O presente trabalho tem como objetivo avaliar diferentes tratamentos químicos alcalinos (NaOH e Ca(OH)2) e biológicos (Penicillium sp. e Aspergillus niger) por microscopia óptica, eletrônica de transmissão e de varredura com o intuito de promover uma alteração física na estrutura da fibra do bagaço de cana-de-açúcar. Os resultados foram avaliados pelas técnicas de microscopia demonstrando que a ação dos tratamentos químicos, principalmente do NaOH + Ca(OH)2, provocou uma eficiente desestruturação das fibras em comparação aos outros tratamentos. Em relação às atividades enzimáticas, o pré-tratamento do bagaço com NaOH +. Ca(OH)2 associado ao A. niger mostrou ser mais eficiente para a produção de lacase. Já para a atividade enzimática de MnP, o tratamento controle (bagaço autoclavado) foi o que apresentou maior eficiência. Para Penicillim sp., a atividade enzimática da lacase não aprsesentou diferença significativa entre os pré-tratamentos bagaço autoclavado e NaOH + Ca(OH)2, no entanto, quando comparados aos pré-tratamentos com NaOH e Ca(OH)2 foram os mais eficientes para a produção dessa enzima. Para a enzima MnP, o pré-tratamento associado às duas bases foi mais eficiente.
Sugarcane is one of the raw materials used for sugar and alcohol production. During the production process, bagasse is generated as a subproduct (or residue), which has a large range of application, as electric power generation, fertilizers, combustible production and animal feeding. Sugarcane bagasse is mainly composed by lignocellulosic material, containing as main component cellulose, hemicelluloses and lignin. Lignin is one of the most recalcitrant naturally found molecules, and, consequently, hardens the access of enzymes to fermentable carbohydrates, decreasing the efficiency of cellulose degradation and fermentation. One of the lignin degradation…
Advisors/Committee Members: Monteiro, Regina Teresa Rosim.
Subjects/Keywords: Aspergillus; Aspergillus niger; Bagaços - Tratamento químico; Cana-de-açúcar; Enzimas; Lignina; Ligninolytic enzymes; Penicillium sp.; Penicillium.; Pre-treatments; Sugarcane bagasse
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❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Fasanella, C. C. (2009). Ação das enzimas ligninolíticas produzidas por Aspergillus niger e Penicillium sp. em bagaço de cana-de-açúcar tratado quimicamente. (Masters Thesis). University of São Paulo. Retrieved from http://www.teses.usp.br/teses/disponiveis/11/11138/tde-10022009-082433/ ;
Chicago Manual of Style (16th Edition):
Fasanella, Cristiane Cipola. “Ação das enzimas ligninolíticas produzidas por Aspergillus niger e Penicillium sp. em bagaço de cana-de-açúcar tratado quimicamente.” 2009. Masters Thesis, University of São Paulo. Accessed January 16, 2021.
http://www.teses.usp.br/teses/disponiveis/11/11138/tde-10022009-082433/ ;.
MLA Handbook (7th Edition):
Fasanella, Cristiane Cipola. “Ação das enzimas ligninolíticas produzidas por Aspergillus niger e Penicillium sp. em bagaço de cana-de-açúcar tratado quimicamente.” 2009. Web. 16 Jan 2021.
Vancouver:
Fasanella CC. Ação das enzimas ligninolíticas produzidas por Aspergillus niger e Penicillium sp. em bagaço de cana-de-açúcar tratado quimicamente. [Internet] [Masters thesis]. University of São Paulo; 2009. [cited 2021 Jan 16].
Available from: http://www.teses.usp.br/teses/disponiveis/11/11138/tde-10022009-082433/ ;.
Council of Science Editors:
Fasanella CC. Ação das enzimas ligninolíticas produzidas por Aspergillus niger e Penicillium sp. em bagaço de cana-de-açúcar tratado quimicamente. [Masters Thesis]. University of São Paulo; 2009. Available from: http://www.teses.usp.br/teses/disponiveis/11/11138/tde-10022009-082433/ ;

Universidade Estadual de Campinas
8.
Lacerda Júnior, Gileno Vieira, 1987-.
Caracterização do microbioma de solos semiáridos da Caatinga e seu potencial para a degradação de lignocelulose: Characterization of the microbiome of Caatinga semiarid soils and its potential for lignocellulose degradation.
Degree: 2017, Universidade Estadual de Campinas
URL: http://repositorio.unicamp.br/jspui/handle/REPOSIP/331987
► Abstract: Caatinga is an exclusively Brazilian biome, poorly studied and preserved, with an exuberant and exclusive biological diversity. This ecosystem consists of a dry tropical…
(more)
▼ Abstract: Caatinga is an exclusively Brazilian biome, poorly studied and preserved, with an exuberant and exclusive biological diversity. This ecosystem consists of a dry tropical forest adapted to severe climatic conditions, including high temperatures and solar irradiation, soil acidity, and water deficit caused by the seasonal rainfall regime. Therefore, it is expected to harbor a soil microbiota carrying unique and versatile enzymatic arsenals with a potential biotechnological application. Resident soil microorganisms are capable of decomposing lignin (second most abundant biopolymer of the biosphere) using a set of
ligninolytic enzymes which can also be applied in the treatment of effluents, pulp and paper industry, biofuels productions, among other industrial processes. Considering that about 99% of soil microorganisms cannot be isolated by conventional culture methods, molecular approaches based on the direct extraction of environmental genetic material, such as "metagenomics", are needed to provide more precise information about the diversity and biotechnological potential of the native microbial community. Thus, this study uses an integrated approach of functional (based on biological activity and sequence similarity) and descriptive metagenomics, with the aim of identifying new lignocellulolytic
enzymes, in addition to analyzing the impact of seasonality and agricultural practices (irrigation/fertilization) in the community structure and functional responses of the Caatinga soil microbiome. The functional screening based on the phenotypic expression of 40,000 metagenomic clones did not show any
ligninolytic activity. However, the molecular screening by PCR allowed the identification of a bacterial Laccase-like Multicopper oxidase, affiliated with the genus Mesorhizobium. The large-scale sequencing of a soil metagenomic library and subsequent in silico analysis revealed a large enzymatic repertoire related to the lignocellulosic degradation carried by different bacterial groups with potential application in the biofuels production as well as other environmental processes. In an ecological approach, it was observed the enrichment of the phyla Actinobacteria and Proteobacteria in the drought and rainy seasonal period, respectively. The data suggest that microorganisms thriving on native soils of the dry period are specialized in monosaccharides and disaccharides utilization, as well as osmoprotective sugars such as trehalose. In contrast, the soil microbiome affected by land use was mainly driven by chemical factors, with the enrichment of the Acidobacteria phylum and genes related to nitrification and denitrification processes, and to oxidative stress. These results suggest that the nutrients input and the breakdown of the natural cycles of the water regime may impact the biogeochemical cycles, affecting the natural functionality of the Caatinga semiarid soils
Advisors/Committee Members: UNIVERSIDADE ESTADUAL DE CAMPINAS (CRUESP), Oliveira, Valeria Maia de, 1966- (advisor), Universidade Estadual de Campinas. Instituto de Biologia (institution), Programa de Pós-Graduação em Genética e Biologia Molecular (nameofprogram), Souza, Anete Pereira de (committee member), Vicentini, Renato (committee member), Mendes, Rodrigo (committee member), Andreote, Fernando Dini (committee member).
Subjects/Keywords: Caatinga; Micro-organismos do solo; Metagenômica; Bioprospecção; Enzimas ligninolíticas; Caatinga; Soil microbiology; Metagenomics; Bioprospecting; Ligninolytic enzymes
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❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Lacerda Júnior, Gileno Vieira, 1. (2017). Caracterização do microbioma de solos semiáridos da Caatinga e seu potencial para a degradação de lignocelulose: Characterization of the microbiome of Caatinga semiarid soils and its potential for lignocellulose degradation. (Thesis). Universidade Estadual de Campinas. Retrieved from http://repositorio.unicamp.br/jspui/handle/REPOSIP/331987
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Lacerda Júnior, Gileno Vieira, 1987-. “Caracterização do microbioma de solos semiáridos da Caatinga e seu potencial para a degradação de lignocelulose: Characterization of the microbiome of Caatinga semiarid soils and its potential for lignocellulose degradation.” 2017. Thesis, Universidade Estadual de Campinas. Accessed January 16, 2021.
http://repositorio.unicamp.br/jspui/handle/REPOSIP/331987.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Lacerda Júnior, Gileno Vieira, 1987-. “Caracterização do microbioma de solos semiáridos da Caatinga e seu potencial para a degradação de lignocelulose: Characterization of the microbiome of Caatinga semiarid soils and its potential for lignocellulose degradation.” 2017. Web. 16 Jan 2021.
Vancouver:
Lacerda Júnior, Gileno Vieira 1. Caracterização do microbioma de solos semiáridos da Caatinga e seu potencial para a degradação de lignocelulose: Characterization of the microbiome of Caatinga semiarid soils and its potential for lignocellulose degradation. [Internet] [Thesis]. Universidade Estadual de Campinas; 2017. [cited 2021 Jan 16].
Available from: http://repositorio.unicamp.br/jspui/handle/REPOSIP/331987.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Lacerda Júnior, Gileno Vieira 1. Caracterização do microbioma de solos semiáridos da Caatinga e seu potencial para a degradação de lignocelulose: Characterization of the microbiome of Caatinga semiarid soils and its potential for lignocellulose degradation. [Thesis]. Universidade Estadual de Campinas; 2017. Available from: http://repositorio.unicamp.br/jspui/handle/REPOSIP/331987
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
9.
Vieira, Gabriela Alves Licursi [UNESP].
Destoxificação e descoloração de poluentes ambientais por consórcios microbianos marinhos.
Degree: 2017, Universidade Estadual Paulista
URL: http://hdl.handle.net/11449/148844
► Considerando a importância dos processos de biorremediação, o presente trabalho teve como objetivo principal avaliar o potencial de consórcios microbianos, constituídos por quatro fungos filamentosos…
(more)
▼ Considerando a importância dos processos de biorremediação, o presente trabalho teve como objetivo principal avaliar o potencial de consórcios microbianos, constituídos por quatro fungos filamentosos isolados de invertebrados marinhos e duas bactérias recuperadas de reservatório de petróleo, na destoxificação de poluentes ambientais, por meio da sobrevivência do microcrustáceo Artemia sp. e da luminescência da bactéria Vibrio fischeri. Após estudos de antagonismo entre os micro-organismos, foram estruturados oito consórcios microbianos, em diferentes combinações. Os consórcios foram usados, isoladamente, para avaliar a toxicidade dos poluentes ambientais corante têxtil RBBR (Remazol Brilliant Blue R), óleo diesel e hidrocarbonetos policíclicos aromáticos. Dentre os consórcios estudados, o consórcio 3, composto por dois fungos filamentosos e pelas duas bactérias, apresentou melhores resultados de destoxificação do corante RBBR e óleo diesel, após sete dias de cultivo a 28 °C e 140 rpm. As enzimas ligninolíticas apresentaram atividade baixa ou ausente no teste feito com o corante e com o óleo diesel. Para o benzo[a]pireno (BaP), apesar de ter havido uma produção mais expressiva de enzimas ligninolíticas, os resultados de destoxificação foram incoerentes, por não apresentar toxicidade mesmo em elevadas concentrações. Sendo assim, no presente estudo o consórcio 3 foi empregado na otimização da destoxificação e descoloração do corante RBBR. Os resultados de dois planejamentos do tipo Plackett-Burman (PB1 e PB2) e um DCCR (Delineamento Composto Central Rotacional) revelaram que as melhores condições de destoxificação/descoloração foram obtidas no ensaio 11 do PB1 e no ensaio 2 do PB2. As condições de cultivo desses dois ensaios foram submetidas a experimentos de validação, porém somente a condição do ensaio 2 do PB2 (inóculo de 4 cilindros de 0,5 cm de cada fungo e 4 mL de cada bactéria (DO 0,4), 1 g/50 mL de farelo de trigo, 100 % de água do mar, pH 8 e 500 ppm de RBBR) apresentou resultados coerentes (52 % de destoxificação e 87 % de descoloração). A atividade de enzimas ligninolíticas na validação foi baixa ou ausente. As análises de FTIR (Fourier Transform Infrared Spectroscopy) resultaram em diferenças no padrão de bandas do corante, quando comparadas às bandas do bioensaio e do controle, indicando atuação dos micro-organismos no processo de descoloração. Os estudos dos metatranscriptomas do experimento de validação (bioensaio e controle) revelaram diferenças na expressão gênica de enzimas específicas envolvidas no processo de degradação de compostos xenobióticos, com destaque para dioxigenase e mono-oxigenases. Tais resultados evidenciam o papel do consórcio microbiano, principalmente das bactérias, na destoxificação, descoloração e degradação do corante têxtil RBBR. Os resultados do presente trabalho demonstram a importância da aplicação do planejamento experimental e da metatranscriptômica para o estudo de destoxificação e degradação de poluentes ambientais, abrindo perspectivas para novos estudos envolvendo os…
Advisors/Committee Members: Sette, Lara Durães [UNESP], Universidade Estadual Paulista (UNESP).
Subjects/Keywords: Poluentes ambientais; Consórcios microbianos; Biodegradação; Toxicidade aguda; Fungos derivados de ambiente marinho; Enzimas ligninolíticas; Environmental pollutants; Microbial consortia; Biodegradation; Acute toxicity; Marine-derived fungi; Ligninolytic enzymes
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❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Vieira, G. A. L. [. (2017). Destoxificação e descoloração de poluentes ambientais por consórcios microbianos marinhos. (Thesis). Universidade Estadual Paulista. Retrieved from http://hdl.handle.net/11449/148844
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Vieira, Gabriela Alves Licursi [UNESP]. “Destoxificação e descoloração de poluentes ambientais por consórcios microbianos marinhos.” 2017. Thesis, Universidade Estadual Paulista. Accessed January 16, 2021.
http://hdl.handle.net/11449/148844.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Vieira, Gabriela Alves Licursi [UNESP]. “Destoxificação e descoloração de poluentes ambientais por consórcios microbianos marinhos.” 2017. Web. 16 Jan 2021.
Vancouver:
Vieira GAL[. Destoxificação e descoloração de poluentes ambientais por consórcios microbianos marinhos. [Internet] [Thesis]. Universidade Estadual Paulista; 2017. [cited 2021 Jan 16].
Available from: http://hdl.handle.net/11449/148844.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Vieira GAL[. Destoxificação e descoloração de poluentes ambientais por consórcios microbianos marinhos. [Thesis]. Universidade Estadual Paulista; 2017. Available from: http://hdl.handle.net/11449/148844
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
10.
Arantes, Valdeir.
Caracterização de compostos de baixa massa molar redutores de ferro produzidos por fungos e mediação da reação de fenton para degradação de polissacarídeos e lignina.
Degree: PhD, Microbiologia Aplicada, 2008, University of São Paulo
URL: http://www.teses.usp.br/teses/disponiveis/97/97132/tde-20082013-170539/
;
► Os fungos de decomposição branca e parda produzem enzimas para degradar os componentes da madeira, os primeiros produzem enzimas hidrolíticas e oxidativas enquanto os outros…
(more)
▼ Os fungos de decomposição branca e parda produzem enzimas para degradar os componentes da madeira, os primeiros produzem enzimas hidrolíticas e oxidativas enquanto os outros produzem principalmente enzimas hidrolíticas. A degradação de polissacarídeos e lignina por fungos de decomposição parda e branca seletiva, respectivamente, não ocorre na região imediata à hifa, e, também, não pode ser explicada unicamente por ação enzimática devido a impermeabilidade das mesmas na parede celular. Neste trabalho estudou-se o sistema degradativo não enzimático envolvendo compostos de baixa massa molar (CBMM) redutores de ferro em fungos degradadores de madeira. O fungo de decomposição parda, Wolfiporia cocos e o de decomposição branca seletiva, Perenniporia medulla-panis foram cultivados em diferentes concentrações de ferro, e a atividade redutora de Fe3+ micelial e a extracelular, assim como a produção de quelantes específicos de ferro, como derivados de ácido hidroxâmico e de catecol, foram induzidas em condição de deficiência de ferro. Os géis de SDS-PAGE dos extratos fúngicos mostraram várias proteínas negativamente reguladas por ferro em P. medulla-panis e W. cocos, principalmente para proteínas entre 10 - 30 kDa. Quando os fungos foram cultivados em diferentes fontes de carbono simples com e sem suplementação de celulose microcristalina e deficiência de ferro, produziram CBMM redutores de Fe3+, os quais tiveram a produção estimulada nos meios com celulose. Análises de eletroforese capilar dos compostos quelantes de metal extraídos dos meios que proporcionaram a maior atividade redutora (Lornitina/ celulose para P. medulla-panis e glicose/celulose para W. cocos) na presença e ausência de ferro, confirmaram que, principalmente P. medulla-panis produz compostos extracelulares que são regulados por ferro. Os CBMM purificados das frações < 5 kDa apresentaram atividade redutora de Fe3+ em pH 2,0 mesmo quando ácido oxálico foi adicionado na concentração 20 vezes maior que a concentração de Fe3+. Em pH 4,5, a atividade redutora foi detectada até uma concentração de ácido oxálico 10 vezes superior a de Fe3+. Em ambos os casos a atividade redutora foi observada quando Fe3+ estava presente na forma livre ou complexada como Fe(oxalato)+. Dentre os vários CBMM produzidos por P. medulla-panis e W. cocos aqueles com atividade redutora foram o ácido 4-hidroxi-fenilacético, 1,2-dihidroxi-3-metil-benzeno, 1,2,3-trihidroxi-benzeno e o ácido 4- hidroxi-cinâmico para W. cocos, e para P. medulla-panis os principais foram 1,2-dihidroxibenzeno e 1,2,3-trihidroxi-benzeno. Além desses compostos, ambos os fungos produziram peptídeos de baixa massa molar com atividade redutora. Os CBMM redutores de Fe3+ de P. medulla-panis (Pmp) e de W. cocos (Wc) foram utilizados na ausência e presença de Fe3+ e H2O2 (reação de Fenton mediada) para oxidar polissacarídeos e lignina in vitro. Verificou-se que os maiores níveis de oxidação foram obtidos nas reações de Fenton mediadas (Wc-Fe3+/H2O2 e Pmp-Fe3+/H2O2). A degradação da celulose por estes sistemas foi…
Advisors/Committee Members: Milagres, Adriane Maria Ferreira.
Subjects/Keywords: Biodegradação; Biodegradation; Compostos redutores de ferro; Enzimas ligninolíticas; Fenton reaction; Fungos degradadores de madeira; Iron reducing compounds; Ligninolytic enzymes; Perenniporia medulla-panis; Perenniporia medullapanis; Reação de Fenton; Wolfiporia cocos; Wolfiporia cocos; Wood decaying fungi
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❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Arantes, V. (2008). Caracterização de compostos de baixa massa molar redutores de ferro produzidos por fungos e mediação da reação de fenton para degradação de polissacarídeos e lignina. (Doctoral Dissertation). University of São Paulo. Retrieved from http://www.teses.usp.br/teses/disponiveis/97/97132/tde-20082013-170539/ ;
Chicago Manual of Style (16th Edition):
Arantes, Valdeir. “Caracterização de compostos de baixa massa molar redutores de ferro produzidos por fungos e mediação da reação de fenton para degradação de polissacarídeos e lignina.” 2008. Doctoral Dissertation, University of São Paulo. Accessed January 16, 2021.
http://www.teses.usp.br/teses/disponiveis/97/97132/tde-20082013-170539/ ;.
MLA Handbook (7th Edition):
Arantes, Valdeir. “Caracterização de compostos de baixa massa molar redutores de ferro produzidos por fungos e mediação da reação de fenton para degradação de polissacarídeos e lignina.” 2008. Web. 16 Jan 2021.
Vancouver:
Arantes V. Caracterização de compostos de baixa massa molar redutores de ferro produzidos por fungos e mediação da reação de fenton para degradação de polissacarídeos e lignina. [Internet] [Doctoral dissertation]. University of São Paulo; 2008. [cited 2021 Jan 16].
Available from: http://www.teses.usp.br/teses/disponiveis/97/97132/tde-20082013-170539/ ;.
Council of Science Editors:
Arantes V. Caracterização de compostos de baixa massa molar redutores de ferro produzidos por fungos e mediação da reação de fenton para degradação de polissacarídeos e lignina. [Doctoral Dissertation]. University of São Paulo; 2008. Available from: http://www.teses.usp.br/teses/disponiveis/97/97132/tde-20082013-170539/ ;
11.
Δρίτσα, Βασιλική.
Μελέτη της αποδόμησης τοξικών ουσιών με λιγνινολυτικούς μύκητες.
Degree: 2006, National Technical University of Athens (NTUA); Εθνικό Μετσόβιο Πολυτεχνείο (ΕΜΠ)
URL: http://hdl.handle.net/10442/hedi/16202
► The scope of this study was the characterization of new strains that produce high ligninolytic activities for their potential use in bioremediation applications. Most of…
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▼ The scope of this study was the characterization of new strains that produce high ligninolytic activities for their potential use in bioremediation applications. Most of the strains were supplied by the ATHUM collection of mycetes from the University of Athens and additionally a few strains were isolated in pure culture in our laboratory. These strains were screened using the standard decolourisation method of Poly R-478, due to its correlation with the ligninolytic degradation activity and the decolourisation of the dye. Grounded on the growth rates in Petri dishes in an appropriate medium, the decolourisation ability and tolerance against two pesticides (PCP and Lindane), eight fungi were selected for further research. To determine the effect of nitrogen as a nutrient to decolourisation in the solid medium BM, two different concentrations of asparagine were used. Then, the ability of eight strains of fungi to decolourise the polymeric dye, Poly R-478, was studied in liquid cultures. The purpose was to evaluate the decolourisation potential of the selected fungal strains in different conditions. To determine the effect of nitrogen content, decolourisation was examined in N- limited and N- rich cultures. Differences were revealed for the tested strains in their ability to decolourise the polymeric dye. The ligninolytic activities of selected strains were determined in a nitrogen-sufficient liquid medium. In order to determine the effect of temperature in ligninolytic activities, the white rot fungi were incubated in different cultivation temperatures (15, 20, 25 and 30 οC). The activities of manganese peroxidase and laccase were determined, but no lignin peroxidase was detected for all strains. Significant differences were revealed between strains of the same species. The polymerase chain reaction (PCR) was used to determine the presence of laccase, lignin and manganese peroxidase potential-producing abilities of fungi. According to available nucleotide sequences of genes from basidiomycetes, one known pair of primers for laccase, three pairs of primers for manganese peroxidase and one pair of primers for lignin peroxidase were used. Additionally, pair of primers for laccasse and two pairs of primers for manganese peroxidase were designed. Finally, it was studied the biodegradation capability of the organochlorine pesticide Lindane by the most effective strains, Pleurotus ostreatus and Ganoderma australe. The central composite design selected as a response surface methodology proved to be suitable for performing bioremediation studies in complex systems in which the growth of microorganisms in a hostile medium, the production of degradative enzymes by the microorganism, the toxicity of the pollutant to the microorganism, the heterogeneity of the system’s components and other factors do not permit a straightforward study. The strains removed Lindane effectively in liquid agitated cultures and their application to bioremediate Lindane contaminated liquid systems is thus a feasible option due to their removal…
Subjects/Keywords: Βιοεξυγίανση; Βιοαποδόμηση; Λιγνινολυτικά ένζυμα; Λακκάση; Υπεροξείδαση μαγγανίου; Σύνθετος κεντρικός σχεδιασμός; Βασιδιομύκητες; Bioremediation; Biodegradation; Ligninolytic enzymes; Laccase; Magnanese peroxidase; Central composite design; Basidiomycetes; Lindane
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APA (6th Edition):
Δρίτσα, . . (2006). Μελέτη της αποδόμησης τοξικών ουσιών με λιγνινολυτικούς μύκητες. (Thesis). National Technical University of Athens (NTUA); Εθνικό Μετσόβιο Πολυτεχνείο (ΕΜΠ). Retrieved from http://hdl.handle.net/10442/hedi/16202
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Δρίτσα, Βασιλική. “Μελέτη της αποδόμησης τοξικών ουσιών με λιγνινολυτικούς μύκητες.” 2006. Thesis, National Technical University of Athens (NTUA); Εθνικό Μετσόβιο Πολυτεχνείο (ΕΜΠ). Accessed January 16, 2021.
http://hdl.handle.net/10442/hedi/16202.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Δρίτσα, Βασιλική. “Μελέτη της αποδόμησης τοξικών ουσιών με λιγνινολυτικούς μύκητες.” 2006. Web. 16 Jan 2021.
Vancouver:
Δρίτσα . Μελέτη της αποδόμησης τοξικών ουσιών με λιγνινολυτικούς μύκητες. [Internet] [Thesis]. National Technical University of Athens (NTUA); Εθνικό Μετσόβιο Πολυτεχνείο (ΕΜΠ); 2006. [cited 2021 Jan 16].
Available from: http://hdl.handle.net/10442/hedi/16202.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Δρίτσα . Μελέτη της αποδόμησης τοξικών ουσιών με λιγνινολυτικούς μύκητες. [Thesis]. National Technical University of Athens (NTUA); Εθνικό Μετσόβιο Πολυτεχνείο (ΕΜΠ); 2006. Available from: http://hdl.handle.net/10442/hedi/16202
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
.