Development of Ready-to-Use Biosensors for Diagnostics and Biosensing.
Ideally, every person in the world should have access to a safe and clean water supply; if not all sources of water are clean and safe, at the very least, an effective method to detect water contamination should be readily available. An effective detection method should not only be sensitive, rapid, robust, and affordable, but, ideally, it should also be equipment-free and easy to transport and deliver to the end-users.
The main goal of this project is to develop a variety of bits and pieces of bioassay systems, with a particular focus on paper-based bioactive devices in order to provide portable and ready-to-use biosensors which can be useable by anyone anywhere around the world without requiring formal training.
According to the World Health Organization (WHO), 76,000 people each year die in India alone because of pesticide poisoning. Long term exposure to organophosphate pesticides is known to have adverse effects on neurological function and can lead to Alzheimer's Disease, Attention Deficit Hyperactivity Disorder (ADHD), and reduced Intelligence Quotient (IQ). The likelihood of long term exposure to pesticides is heightened in developing countries, so a reliable and inexpensive pesticide sensor is a much-needed device in the developing world. To address this need, this project reports on the development of a fully-automated bioactive paper-based sensor for the detection of organophosphate pesticides. In the proposed biosensor, two innovations were implemented to achieve a full-automated format for the pesticide sensor: (I) First is a PUMP ON A PAPER (Jahanshahi-Anbuhi et al., LOC, 2012) that increases the flow rate of fluids within paper-based microfluidic analytical devices and sequentially brings two separate liquid streams to the enzyme test zone on the paper sensor, and (II) the second innovation is a PIPETTE ON A PAPER (Jahanshahi-Anbuhi et al., LOC, 2014) that involved the creation of a pullulan (a natural non-ionic polysaccharide) temporary bridge-system to transfer a known amount of solution to the sensing zone that, gives the enzyme zone a chance to dry and accept the substrate solution from the slow channel after a fixed period of time. This proposed format results in a simplified assay that detects the presence of pesticides automatically without any further manipulation from the user.
However, the shelf life of this assay kit is challenging due to instability of both enzyme (AChE) and substrate (IDA) at room temperature. AChE loses its enzymatic activity when stored at room temperature and IDA becomes oxidized quickly. This problem is not unique to these two bio reagents, however; almost all bioassays which use bio-reagents (such as enzymes and small-molecular substrates) are unstable to varying degrees and require special shipping and storage. The instability of these molecules can arise from either thermal denaturation or chemical modification, such as oxidation or hydrolysis. Because of these issues, they often have to be shipped on dry ice with special packaging, which is costly. The cost…
Advisors/Committee Members: Pelton, Robert, Filipe, Carlos D.M., Chemical Engineering.
to Zotero / EndNote / Reference
APA (6th Edition):
Jahanshahi-Anbuhi, S. (2014). Development of Ready-to-Use Biosensors for Diagnostics and Biosensing. (Doctoral Dissertation). McMaster University. Retrieved from http://hdl.handle.net/11375/16501
Chicago Manual of Style (16th Edition):
Jahanshahi-Anbuhi, Sana. “Development of Ready-to-Use Biosensors for Diagnostics and Biosensing.” 2014. Doctoral Dissertation, McMaster University. Accessed October 23, 2019.
MLA Handbook (7th Edition):
Jahanshahi-Anbuhi, Sana. “Development of Ready-to-Use Biosensors for Diagnostics and Biosensing.” 2014. Web. 23 Oct 2019.
Jahanshahi-Anbuhi S. Development of Ready-to-Use Biosensors for Diagnostics and Biosensing. [Internet] [Doctoral dissertation]. McMaster University; 2014. [cited 2019 Oct 23].
Available from: http://hdl.handle.net/11375/16501.
Council of Science Editors:
Jahanshahi-Anbuhi S. Development of Ready-to-Use Biosensors for Diagnostics and Biosensing. [Doctoral Dissertation]. McMaster University; 2014. Available from: http://hdl.handle.net/11375/16501