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You searched for subject:(LSCFB). Showing records 1 – 3 of 3 total matches.

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University of Western Ontario

1. Alassuity, Ahmed. Continuous protein refolding in a liquid solid circulating fluidized bed (LSCFB) system.

Degree: 2017, University of Western Ontario

Biotechnology has significantly contributed to the production of food, drugs, and a lot of specialty and chemical products. In addition to that, biotechnology provided and can still provide sustainable, and green, solutions to many environmental problems. By using recombinant DNA technology, almost any protein can be cloned and produced in large quantities. The major challenge facing this type of a protein production approach is the need for proper refolding of target protein. The main objective of the current study is to provide the biotechnology industries with a more efficient and economical technology for continuous protein purification and refolding. The Circulating fluidized bed technology has been selected as a promising alternative to the current practice in protein purification and refolding. The circulating fluidized bed technology offers many advantages over conventional technique such as better heat and mass transfer, better solid liquid contact, ability to operate in continuous mode, the ability to handle two main liquid streams without mixing. During the course of this study matrix-assisted protein refolding using Immobilized Metal affinity chromatography (IMAC) was successfully used for purification and refolding of recombinant GST-His6 protein. The recombinant protein was produced in genetically modified E. coli and the over expression of target protein was induced by IPTG. In addition, two high density composite IMAC beads have been successfully designed and constructed. The main components in IMAC beads are biopolymer agarose and a filler. The biopolymer outer layer was derivatized to introduce the IMAC chelating moiety IDA. The constructed composite IMAC beads were fully characterized by SEM, FTIR, Master Sizer, and XPS to confirm the chemical structure of the derivatized biopolymer layer on the beads surface. Other commercially available IMAC beads were also evaluate during the course of this study. A new scale-down design of the Liquid solid circulated fluidized bed system was constructed to investigate the developed protein purification and refolding approach in a continuous operation mode. The new LSCFB system has a simple design with some unique features. The total volume of the new LSCFB system is about 2.85 L and can handle up to 1 kg of Ion-exchange beads in a continuous mode. The system was successfully used for purification and refolding of His6-tagged recombinant proteins. The new system can also be applied for purification of many other biological products. The newly designed LSCFB system has high potential in environmental applications.

Subjects/Keywords: Protein refolding; Circulating fluidized bed; IMAC beads; composite beads; matrix-assisted protein refolding; LSCFB; Biochemical and Biomolecular Engineering

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6th Edition):

Alassuity, A. (2017). Continuous protein refolding in a liquid solid circulating fluidized bed (LSCFB) system. (Thesis). University of Western Ontario. Retrieved from https://ir.lib.uwo.ca/etd/4756

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16th Edition):

Alassuity, Ahmed. “Continuous protein refolding in a liquid solid circulating fluidized bed (LSCFB) system.” 2017. Thesis, University of Western Ontario. Accessed June 25, 2019. https://ir.lib.uwo.ca/etd/4756.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7th Edition):

Alassuity, Ahmed. “Continuous protein refolding in a liquid solid circulating fluidized bed (LSCFB) system.” 2017. Web. 25 Jun 2019.

Vancouver:

Alassuity A. Continuous protein refolding in a liquid solid circulating fluidized bed (LSCFB) system. [Internet] [Thesis]. University of Western Ontario; 2017. [cited 2019 Jun 25]. Available from: https://ir.lib.uwo.ca/etd/4756.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Alassuity A. Continuous protein refolding in a liquid solid circulating fluidized bed (LSCFB) system. [Thesis]. University of Western Ontario; 2017. Available from: https://ir.lib.uwo.ca/etd/4756

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation


University of Western Ontario

2. Luo, Hao. Numerical Simulations of Two-Phase Flows in the Liquid Solid Circulating Fluidized Bed.

Degree: 2017, University of Western Ontario

The liquid-solid circulating fluidized bed (LSCFB) has many potential applications in biochemical and petroleum industries, as well as in wastewater treatments, given its higher contact efficiency and being able to accommodate two reactions under one system. With extensive experimental results becoming available, there is clearly a need for computational fluid dynamics (CFD) modeling to expand our understandings of LSCFBs and to predict the hydrodynamic behaviors of the two-phase flows within LSCFB. In this research, the Eulerian-Eulerian two-phase model combined with the kinetic theory for the granular phase is applied to simulate the two-phase flows in LSCFBs. The key factors affecting the simulation results including the drag model, near wall treatment and boundary condition are investigated and the CFD model is validated by comparing the numerical results with the experimental data. Then, the hydrodynamics of LSCFBs under different operating conditions are investigated numerically. Among the seven different drag models examined in this study, the adjusted Syamlal O’Brien drag model and the irregular particle drag model were found to provide the best numerical solutions for spherical and irregular particles, respectively. For the three different near wall treatments tested, the Menter-Lechner near wall treatment was found to provide the best predictions for the near wall region. It is also found that the numerical results are insensitive to the restitution and specularity coefficients, which are used in the boundary conditions for the solid phase. In addition, the proposed CFD model with the best drag model and near wall treatment is applied to simulate the two-phase flows in LSCFBs under different operating conditions, including different superficial liquid velocities, superficial solid velocities and particle densities. The numerical predictions show correct trends and good agreements with the experimental data.

Subjects/Keywords: Numerical Simulation; Computational Fluid Dynamics (CFD); Liquid-Solid Circulating Fluidized Bed (LSCFB); Drag Model; Near Wall Treatment; Multiphase Flow; Other Mechanical Engineering

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6th Edition):

Luo, H. (2017). Numerical Simulations of Two-Phase Flows in the Liquid Solid Circulating Fluidized Bed. (Thesis). University of Western Ontario. Retrieved from https://ir.lib.uwo.ca/etd/4918

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16th Edition):

Luo, Hao. “Numerical Simulations of Two-Phase Flows in the Liquid Solid Circulating Fluidized Bed.” 2017. Thesis, University of Western Ontario. Accessed June 25, 2019. https://ir.lib.uwo.ca/etd/4918.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7th Edition):

Luo, Hao. “Numerical Simulations of Two-Phase Flows in the Liquid Solid Circulating Fluidized Bed.” 2017. Web. 25 Jun 2019.

Vancouver:

Luo H. Numerical Simulations of Two-Phase Flows in the Liquid Solid Circulating Fluidized Bed. [Internet] [Thesis]. University of Western Ontario; 2017. [cited 2019 Jun 25]. Available from: https://ir.lib.uwo.ca/etd/4918.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Luo H. Numerical Simulations of Two-Phase Flows in the Liquid Solid Circulating Fluidized Bed. [Thesis]. University of Western Ontario; 2017. Available from: https://ir.lib.uwo.ca/etd/4918

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

3. Sang, Long. Particle Fluidization in Upward and Inverse Liquid-Solid Circulating Fluidized Bed.

Degree: 2013, University of Western Ontario

The fluidization of particles in upwards and inverse Liquid-Solid Circulating Fluidized Bed is carried out to investigate the hydrodynamic characteristics when using “heavy” and “light” particles, whose densities are higher and lower than that of the surrounding liquid respectively. Generally, the solids are fluidized upwards in the former case, whereas, the downwards fluidization is preferred in the latter scenario. In the Liquid-Solid Circulating Fluidized Bed (LSCFB) riser, where the upwards fluidization takes place, the effects of particle properties on solids holdup are investigated experimentally based on three parameters: superficial liquid velocity, normalized superficial liquid velocity and excess superficial liquid velocity. The results show that the excess superficial liquid velocity (Ul-Ut), among those three parameters, is a more appropriate parameter to evaluate the effects of the particle properties on the solids holdup, facilitating general comparisons for different types of particles. Then such particle property effects are studied analytically by incorporating operating parameters and particle properties into a mathematical model, showing excellent agreement with the experimental results. By this model, the transition velocity demarcating the circulating fluidization regime and the transport regime is determined to complete the flow regime map in liquid-solid fluidization systems. In the Inverse Liquid-Solid Circulating Fluidized Bed (ILSCFB) downer, where the inverse fluidization takes place, under the circulating fluidization regime, the hydrodynamic characteristics are investigated experimentally by the fluidization of Styrofoam and Hollow Glassbeads. For both types of particles, axial solids holdup distribution is quite uniform, while radial solids holdup distribution is slightly non-uniform with slight increase adjacent to the wall under various operating conditions, but no obvious “core-annulus” structure is observed. Such solids holdup distribution pattern is closely related to the solids circulation rate, superficial liquid velocity, local liquid and particle velocity which are also measured for Styrofoam particles. It is shown that the radial profiles of both liquid and particle velocities are slightly non-uniform, higher at the center region while lower adjacent to the wall, influenced by solids circulation rate and superficial liquid velocity. The local slip velocity derived from local liquid and particle velocities is found to be very close to the single particle terminal velocity and one-dimensional slip velocity deduced from the superficial liquid, solids velocities and cross-sectional average solids holdup, suggesting that there is no obvious clustering phenomenon and solids segregation in ILSCFB downer under various operating conditions. The hydrodynamics under the inverse conventional fluidization regime are also studied by examining the bed voidage and dimensionless bed expansion. A new mathematical model correlating Archimedes and Reynolds number is proposed for the prediction of…

Subjects/Keywords: Liquid-Solid Circulating Fluidized Bed (LSCFB); Inverse Liquid-Solid Circulating Fluidized Bed (ILSCFB); solids holdup; particle properties; liquid velocity; particle velocity; slip velocity; flow regime map; Chemical Engineering; Engineering

…an LSCFB Riser… …43 4.4 The effects of particle properties on the hydrodynamics in LSCFB riser… …66 5 Prediction of Average Solids Holdup and Slip Velocity in LSCFB Riser… …133 7.5 Comparisons of ILSCFB and LSCFB… …175 B1 Superficial solids velocity (Us) in LSCFB and ILSCFB… 

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6th Edition):

Sang, L. (2013). Particle Fluidization in Upward and Inverse Liquid-Solid Circulating Fluidized Bed. (Thesis). University of Western Ontario. Retrieved from https://ir.lib.uwo.ca/etd/1218

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16th Edition):

Sang, Long. “Particle Fluidization in Upward and Inverse Liquid-Solid Circulating Fluidized Bed.” 2013. Thesis, University of Western Ontario. Accessed June 25, 2019. https://ir.lib.uwo.ca/etd/1218.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7th Edition):

Sang, Long. “Particle Fluidization in Upward and Inverse Liquid-Solid Circulating Fluidized Bed.” 2013. Web. 25 Jun 2019.

Vancouver:

Sang L. Particle Fluidization in Upward and Inverse Liquid-Solid Circulating Fluidized Bed. [Internet] [Thesis]. University of Western Ontario; 2013. [cited 2019 Jun 25]. Available from: https://ir.lib.uwo.ca/etd/1218.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Sang L. Particle Fluidization in Upward and Inverse Liquid-Solid Circulating Fluidized Bed. [Thesis]. University of Western Ontario; 2013. Available from: https://ir.lib.uwo.ca/etd/1218

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

.