subject:(LDL)

1. Teixeira, Luciane dos Santos. Estudos das propriedades ópticas dos complexos európio tetraciclinas e suas aplicações na detecção de lipoproteínas.

Degree: Mestrado, Tecnologia Nuclear - Materiais, 2010, University of São Paulo

URL: http://www.teses.usp.br/teses/disponiveis/85/85134/tde-12082011-133857/ ;

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Este trabalho apresenta as propriedades ópticas dos complexos Európio Tetraciclinas (EuTcs) na presença de LDL e de LDL oxidada com potenciais aplicações em análises clínicas.… (more)

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Este trabalho apresenta as propriedades ópticas dos complexos Európio Tetraciclinas (EuTcs) na presença de LDL e de LDL oxidada com potenciais aplicações em análises clínicas. Foram escolhidos quatro elementos da família das Tetraciclinas: Tetraciclina (Tc), Clorotetraciclina (CTc), Metatetraciclina (MTc) e Oxitetraciclina (OTc) para fazerem parte dos complexos com o íon európio. As melhores condições para se formar os complexos eficientemente foram determinadas, através das medidas dos parâmetros ópticos como: absorção, emissão e de tempo de vida. As melhores concentrações de európio nos complexos EuTcs e possíveis influências de íons inorgânicos normalmente presentes no plasma sanguíneo também foram analisadas. As amostras foram preparadas em pH neutro e a luminescência visível do lantanídeo foi detectada após tempo de repouso das amostras de 15 minutos. Os resultados deste trabalho mostraram que as moléculas de LDL e de LDL oxidada apresentaram um importante papel no aumento da intensidade de emissão dos complexos das Tcs. As medidas realizadas com os complexos EuTcs não apresentaram deslocamentos nos comprimentos de onda dos espectros de absorção e de emissão na presença de LDL, o que demonstra a ausência de interação direta entre as moléculas de Tcs e as moléculas de LDL e LDL oxidada. No entanto, o íon európio pode interagir em diferentes sítios das moléculas de tetraciclinas o que diferenciou a intensidade de emissão de cada complexo. Comparando os resultados obtidos entre os complexos de EuTcs, o complexo EuTc foi o que apresentou perspectivas promissoras na quantificação de LDL e LDL oxidada.

This work presents the optical properties of europium complexes - Tetracyclines (EuTcs) in the presence of LDL and oxidized LDL with potential applications in clinical analysis. Four elements were chosen from the Tetracyclines family: Tetracycline (Tc), Chlortetracycline (CTc), Metatetraciclina (MTc) and Oxytetracycline (OTc) to be part of complexes with europium ion. The best conditions to form the complex efficiently were determined through measurements of optical parameters such as absorption, emission and lifetime. The best concentrations of europium complexes in EuTcs and possible influences of inorganic ions normally present in blood plasma were also analyzed. The samples were prepared at neutral pH and the visible luminescence of lanthanide was detected after resting time of the samples of 15 minutes. These results showed that the molecules of LDL and oxidized LDL have an important role in increase of the emission intensity for Tcs complexes . The measurements performed with the complex EuTcs showed no shifts in the wavelengths of the absorption and emission spectra in the presence of LDL, which demonstrates the absence of direct interaction between the molecules of Tcs and the molecules of LDL and oxidized LDL. However, the europium ion can interact at different sites of the tetracyclines molecules which differed the emission intensity of each complex. Comparing the results obtained between the complexes…

Advisors/Committee Members: Courrol, Lilia Coronato.

Subjects/Keywords: európio; europium; LDL; LDL; LDL oxidada; oxidized LDL; tetraciclinas; tetracyclines

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APA (6^th Edition):

Teixeira, L. d. S. (2010). Estudos das propriedades ópticas dos complexos európio tetraciclinas e suas aplicações na detecção de lipoproteínas. (Masters Thesis). University of São Paulo. Retrieved from http://www.teses.usp.br/teses/disponiveis/85/85134/tde-12082011-133857/ ;

Chicago Manual of Style (16^th Edition):

Teixeira, Luciane dos Santos. “Estudos das propriedades ópticas dos complexos európio tetraciclinas e suas aplicações na detecção de lipoproteínas.” 2010. Masters Thesis, University of São Paulo. Accessed January 22, 2021. http://www.teses.usp.br/teses/disponiveis/85/85134/tde-12082011-133857/ ;.

MLA Handbook (7^th Edition):

Teixeira, Luciane dos Santos. “Estudos das propriedades ópticas dos complexos európio tetraciclinas e suas aplicações na detecção de lipoproteínas.” 2010. Web. 22 Jan 2021.

Vancouver:

Teixeira LdS. Estudos das propriedades ópticas dos complexos európio tetraciclinas e suas aplicações na detecção de lipoproteínas. [Internet] [Masters thesis]. University of São Paulo; 2010. [cited 2021 Jan 22]. Available from: http://www.teses.usp.br/teses/disponiveis/85/85134/tde-12082011-133857/ ;.

Council of Science Editors:

Teixeira LdS. Estudos das propriedades ópticas dos complexos európio tetraciclinas e suas aplicações na detecção de lipoproteínas. [Masters Thesis]. University of São Paulo; 2010. Available from: http://www.teses.usp.br/teses/disponiveis/85/85134/tde-12082011-133857/ ;

2. Cohen, Danielle. Lipoproteína de baixa densidade oxidada (LDLox) versus lipoproteína de baixa densidade eletronegativa [LDL(-)] de adolescentes: análise comparativa.

Degree: Mestrado, Nutrição Humana Aplicada, 2013, University of São Paulo

URL: http://www.teses.usp.br/teses/disponiveis/89/89131/tde-19032014-165411/ ;

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A obesidade é considerada uma doença crônica e multifatorial, onde eventos como a inflamação de baixa intensidade e as modificações oxidativas estão presentes. A elevada… (more)

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A obesidade é considerada uma doença crônica e multifatorial, onde eventos como a inflamação de baixa intensidade e as modificações oxidativas estão presentes. A elevada prevalência de obesidade tem impacto direto no desenvolvimento precoce de diabetes mellitus, hipertensão e outros fatores de risco cardiovasculares. Esse perfil tem motivado a identificação de biomarcadores precoces, sendo o monitoramento da lipoproteína de baixa densidade oxidada (LDLox) e lipoproteína de baixa densidade eletronegativa [LDL(-)] potenciais candidatos. Diante do exposto, o objetivo deste estudo foi realizar a análise comparativa e de correlação entre o conteúdo de LDLox e [LDL(-)] em adolescentes. Foram selecionados 137 adolescentes de ambos sexos, com faixa etária de 10 a 19 anos e regularmente em matriculados em escolas públicas da cidade de São Paulo. O peso, altura e circunferência da cintura (CC) foram avaliados. Após jejum (12h-15h) foi coletada uma amostra de sangue e, a partir do plasma, foram realizadas as seguintes análises: glicose, insulina, perfil lipídico, apolipoproteína (AI e B), ácidos graxos não esterificados, tamanho de HDL, atividade da CETP e LDL(-) e LDLox. Os resultados encontrados foram analisados por meio do programa SPSS 15.0, considerando valor de significância de p< 0,05. Os 137 adolescentes foram distribuídos em dois grupos: 71 no Eutrófico (51,82%) e 66 no Obeso (48,18%), segundo a classificação do IMC. 48 (35,04%) dos adolescentes eram do sexo masculino e 89 (64,96%) do sexo feminino, com idade média de 14,2 (2,3) anos. Em relação à CC, observou-se que essa confirmou a classificação feita pelo IMC. Observou-se também uma maior prevalência de hipertensão (65% p = 0,011) e obesidade (64,7% p=0,041) nos antecedentes familiares do grupo Obeso quando comparado ao grupo Eutrófico. Os adolescentes obesos apresentaram maiores valores de triglicerídeos, HDL, APO B, CETP, insulina e LDL(-) e LDLox, quando comparados aos eutróficos. Perfil inverso foi observado para Apo AI. O conteúdo de LDLox e LD(-) variou significativamente em função do IMC. Entretanto, essas partículas de LDLs não se correlacionaram entre si, embora tenham apresentado associação com outros parâmetros cardiometabólicos. Os resultados obtidos confirmam o impacto negativo da obesidade sobre os parâmetros cardiometabólicos de adolescentes e, apesar do conteúdo de LDLox e LDL(-) ter aumentado em função do IMC, essas partículas parecem ser estruturalmente distintas. Essa possibilidade foi reforçada pelas diferentes associações dessas partículas com outros marcadores bioquímicos.

Obesity is considered a chronic and multifactorial disease, where events such as low intensity inflammation and oxidative modifications are present. The high prevalence of obesity has a direct impact on the early development of diabetes mellitus, hypertension and other cardiovascular risk factors. This profile has motivated the identification of early biomarkers, and monitoring the oxidized low density lipoprotein (oxLDL) and electronegative low-density lipoprotein [LDL…

Advisors/Committee Members: Damasceno, Nágila Raquel Teixeira.

Subjects/Keywords: Adolescentes; Adolescents; Electronegative LDL; LDL eletronegativa; LDL oxidada; Obesidade; Obesity; Oxidized LDL

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Cohen, D. (2013). Lipoproteína de baixa densidade oxidada (LDLox) versus lipoproteína de baixa densidade eletronegativa [LDL(-)] de adolescentes: análise comparativa. (Masters Thesis). University of São Paulo. Retrieved from http://www.teses.usp.br/teses/disponiveis/89/89131/tde-19032014-165411/ ;

Chicago Manual of Style (16^th Edition):

Cohen, Danielle. “Lipoproteína de baixa densidade oxidada (LDLox) versus lipoproteína de baixa densidade eletronegativa [LDL(-)] de adolescentes: análise comparativa.” 2013. Masters Thesis, University of São Paulo. Accessed January 22, 2021. http://www.teses.usp.br/teses/disponiveis/89/89131/tde-19032014-165411/ ;.

MLA Handbook (7^th Edition):

Cohen, Danielle. “Lipoproteína de baixa densidade oxidada (LDLox) versus lipoproteína de baixa densidade eletronegativa [LDL(-)] de adolescentes: análise comparativa.” 2013. Web. 22 Jan 2021.

Vancouver:

Cohen D. Lipoproteína de baixa densidade oxidada (LDLox) versus lipoproteína de baixa densidade eletronegativa [LDL(-)] de adolescentes: análise comparativa. [Internet] [Masters thesis]. University of São Paulo; 2013. [cited 2021 Jan 22]. Available from: http://www.teses.usp.br/teses/disponiveis/89/89131/tde-19032014-165411/ ;.

Council of Science Editors:

Cohen D. Lipoproteína de baixa densidade oxidada (LDLox) versus lipoproteína de baixa densidade eletronegativa [LDL(-)] de adolescentes: análise comparativa. [Masters Thesis]. University of São Paulo; 2013. Available from: http://www.teses.usp.br/teses/disponiveis/89/89131/tde-19032014-165411/ ;

University of Debrecen

3. Pethő, Dávid. A kén-hidrogén donor molekulák gátolják az alacsony sűrűségű lipoprotein oxidációját .

Degree: DE – Természettudományi és Technológiai Kar – Biológiai és Ökológiai Intézet, 2014, University of Debrecen

URL: http://hdl.handle.net/2437/191516

► A Vaszkuláris Biológiai Kutatócsoport 9 éve foglalkozik korunk egyik legszámottevőbb népbetegséggel az atherosclerosissal. Kimutatták, hogy a NaHS gátolja a hem mediálta LDL oxidatív modifikációját és… (more)

Subjects/Keywords: Kén-hidrogén; LDL

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APA (6^th Edition):

Pethő, D. (2014). A kén-hidrogén donor molekulák gátolják az alacsony sűrűségű lipoprotein oxidációját . (Thesis). University of Debrecen. Retrieved from http://hdl.handle.net/2437/191516

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Pethő, Dávid. “A kén-hidrogén donor molekulák gátolják az alacsony sűrűségű lipoprotein oxidációját .” 2014. Thesis, University of Debrecen. Accessed January 22, 2021. http://hdl.handle.net/2437/191516.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Pethő, Dávid. “A kén-hidrogén donor molekulák gátolják az alacsony sűrűségű lipoprotein oxidációját .” 2014. Web. 22 Jan 2021.

Vancouver:

Pethő D. A kén-hidrogén donor molekulák gátolják az alacsony sűrűségű lipoprotein oxidációját . [Internet] [Thesis]. University of Debrecen; 2014. [cited 2021 Jan 22]. Available from: http://hdl.handle.net/2437/191516.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Pethő D. A kén-hidrogén donor molekulák gátolják az alacsony sűrűségű lipoprotein oxidációját . [Thesis]. University of Debrecen; 2014. Available from: http://hdl.handle.net/2437/191516

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

4. Favero, Giovani Marino. Nanoemulsão contendo 7-cetocolesterol (LDE/7KC) promove inibição do crescimento de melanoma em camundongos e aumento de sobrevida.

Degree: PhD, Alergia e Imunopatologia, 2007, University of São Paulo

URL: http://www.teses.usp.br/teses/disponiveis/5/5146/tde-12062007-100409/ ;

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7-cetocoleterol (7KC) é um oxisterol conhecido por inibir a proliferação celular e por ser citotóxico. Desenvolvemos uma nanoemulsão contendo 7KC (LDE/7KC) que demonstrou efeito anti-proliferativo… (more)

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7-cetocoleterol (7KC) é um oxisterol conhecido por inibir a proliferação celular e por ser citotóxico. Desenvolvemos uma nanoemulsão contendo 7KC (LDE/7KC) que demonstrou efeito anti-proliferativo sobre as linhagens RPMI 8226 (mieloma) e melanoma (B16F10), in vitro, sendo preferencialmente captada via receptores de LDL. No presente trabalho avaliamos, in vivo, a cinética plasmática, biodistribuição, ação anti-tumoral e parâmetros tóxico-hematológicos em camundongos portadores de melanoma. A cinética plasmática apresentou um decaimento estatisticamente igual entre os animais portadores de melanoma e não portadores. Em relação à biodistribuição da nanoemulsão, houve um acúmulo de seus componentes radioativamente marcados, principalmente no fígado e no tumor, sugerindo sua captação via receptores de LDL. LDE/7KC promoveu uma redução superior a cinqüenta por cento do tamanho do tumor, que apresentou maior área de necrose e menor quantidade de vasos. Nos camundongos tratados com LDE/7KC houve um aumento da sobrevida. As análises toxico-hematológicas demonstraram que a nanoemulsão apresentou pouca ou nenhuma toxicidade. Os resultados demonstram a possibilidade da utilização da nanoemulsão LDE/7KC como um agente no tratamento do câncer, com poucos efeitos colaterais, devido a sua seletividade aos receptores da LDL.

7-ketocholesterol (7KC) is an oxysterol known to inhibit cell proliferation and to be cytotoxic. A nanoemulsion containing-7KC (LDE/7KC) was shown to have antiproliferative effects on RPMI 8226 myeloma cell line and melanoma (B16F10), in vitro. This particle is taken up mainly by LDL receptors. Here we have evaluated the plasma kinetic, biodistribution, anti-tumoral action and hematologic toxicity of LDE/7KC in melanoma bearing mice. The nanoemulsion accumulated in the liver and tumor, tissues with a high expression of LDL receptors. LDE/7KC promoted a tumor size reduction over fifty percent. An increased necrosis area and a decreased amount of blood vessels were found. An increased survival rate was observed. The hematolgic analyses demonstrated a lack of toxicicity. The results shows the possibility to use the LDE/7KC nanoemulsion as an agent for cancer treatment, with few collateral effects probably due to its internalization by LDL receptors.

Advisors/Committee Members: Bydlowski, Sergio Paulo.

Subjects/Keywords: Cetocolesteróis; Emulsions; Emulsões; Farmacocinética; Ketocholesterol; LDL lipoprotein; LDL receptor; Lipoproteínas do colesterol LDL; Melanoma; Melanoma experimental; Pharmacokinetics.; Receptores LDL

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Favero, G. M. (2007). Nanoemulsão contendo 7-cetocolesterol (LDE/7KC) promove inibição do crescimento de melanoma em camundongos e aumento de sobrevida. (Doctoral Dissertation). University of São Paulo. Retrieved from http://www.teses.usp.br/teses/disponiveis/5/5146/tde-12062007-100409/ ;

Chicago Manual of Style (16^th Edition):

Favero, Giovani Marino. “Nanoemulsão contendo 7-cetocolesterol (LDE/7KC) promove inibição do crescimento de melanoma em camundongos e aumento de sobrevida.” 2007. Doctoral Dissertation, University of São Paulo. Accessed January 22, 2021. http://www.teses.usp.br/teses/disponiveis/5/5146/tde-12062007-100409/ ;.

MLA Handbook (7^th Edition):

Favero, Giovani Marino. “Nanoemulsão contendo 7-cetocolesterol (LDE/7KC) promove inibição do crescimento de melanoma em camundongos e aumento de sobrevida.” 2007. Web. 22 Jan 2021.

Vancouver:

Favero GM. Nanoemulsão contendo 7-cetocolesterol (LDE/7KC) promove inibição do crescimento de melanoma em camundongos e aumento de sobrevida. [Internet] [Doctoral dissertation]. University of São Paulo; 2007. [cited 2021 Jan 22]. Available from: http://www.teses.usp.br/teses/disponiveis/5/5146/tde-12062007-100409/ ;.

Council of Science Editors:

Favero GM. Nanoemulsão contendo 7-cetocolesterol (LDE/7KC) promove inibição do crescimento de melanoma em camundongos e aumento de sobrevida. [Doctoral Dissertation]. University of São Paulo; 2007. Available from: http://www.teses.usp.br/teses/disponiveis/5/5146/tde-12062007-100409/ ;

University of Ottawa

5. Sarkar, Samantha Khadija. Asociation of PCSK9 with Low Density Lipoproteins (LDL) in the Regulation of LDL-Cholesterol Levels .

Degree: 2015, University of Ottawa

URL: http://hdl.handle.net/10393/32825

► Proprotein Convertase Subtilisin / Kexin Type-9 (PCSK9) has emerged as a major regulator of plasma cholesterol levels. PCSK9 is secreted mainly from the liver and… (more)

▼ Proprotein Convertase Subtilisin / Kexin Type-9 (PCSK9) has emerged as a major regulator of plasma cholesterol levels. PCSK9 is secreted mainly from the liver and circulates as a plasma protein. PCSK9 binds cell surface low-density lipoprotein (LDL) receptors and mediates their degradation upon endocytosis in the liver. This decreases the liver’s ability to clear LDL-cholesterol from the blood. PCSK9 is also capable of binding LDL particles themselves; this interaction inhibits the ability of PCSK9 to bind and mediate LDLR degradation in cultured hepatic cells, but its effect on PCSK9 function in vivo remains unknown. A disordered N-terminal region of the PCSK9 prodomain is necessary for binding to isolated LDL particles in vitro. This N-terminal region is also autoinhibitory to PCSK9-LDL receptor binding. We hypothesized that the N-terminal of the PCSK9 prodomain plays a role in an allosteric mechanism that regulates PCSK9 function. Through mutagenesis studies, we found that both a conserved stretch of acidic residues and an adjacent conserved stretch of hydrophobic residues are crucial for the PCSK9-LDL interaction; the hydrophobicity of the residue at position 38 (Tyr) within the conserved acidic stretch was also found to be important for this. Helical wheel modeling of the prodomain N-terminal sequence revealed the potential for a lipid-ordered amphipathic helix to form, which may aid PCSK9 docking onto LDL. Replacing residues A44 and L41 with helix-disrupting proline residues abolished LDL binding. Co-pelleting ultracentrifugation assays also show that wild-type PCSK9 is capable of associating with liposomes, while the A44P mutation disrupts this lipid association. The A44P-PCSK9 mutation, showing an 80-90% decrease in LDL association but with LDL receptor binding and degrading functions intact, may serve as an important tool in future studies investigating the PCSK9-LDL interaction in vivo. Elucidation of the mechanism by which LDL-binding naturally inhibits PCSK9 activity may also help to develop new anti-PCSK9 therapeutics in the future.

Subjects/Keywords: PCSK9; LDL; lipoprotein; cholesterol

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Sarkar, S. K. (2015). Asociation of PCSK9 with Low Density Lipoproteins (LDL) in the Regulation of LDL-Cholesterol Levels . (Thesis). University of Ottawa. Retrieved from http://hdl.handle.net/10393/32825

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Sarkar, Samantha Khadija. “Asociation of PCSK9 with Low Density Lipoproteins (LDL) in the Regulation of LDL-Cholesterol Levels .” 2015. Thesis, University of Ottawa. Accessed January 22, 2021. http://hdl.handle.net/10393/32825.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Sarkar, Samantha Khadija. “Asociation of PCSK9 with Low Density Lipoproteins (LDL) in the Regulation of LDL-Cholesterol Levels .” 2015. Web. 22 Jan 2021.

Vancouver:

Sarkar SK. Asociation of PCSK9 with Low Density Lipoproteins (LDL) in the Regulation of LDL-Cholesterol Levels . [Internet] [Thesis]. University of Ottawa; 2015. [cited 2021 Jan 22]. Available from: http://hdl.handle.net/10393/32825.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Sarkar SK. Asociation of PCSK9 with Low Density Lipoproteins (LDL) in the Regulation of LDL-Cholesterol Levels . [Thesis]. University of Ottawa; 2015. Available from: http://hdl.handle.net/10393/32825

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Gothenburg / Göteborgs Universitet

6. Cramér, Kim, 1924-1992. Studies on human serum §-lipoproteins, including their protein moiety.

Degree: 1961, University of Gothenburg / Göteborgs Universitet

URL: http://hdl.handle.net/2077/17427

Subjects/Keywords: Lipoproteins; LDL

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Cramér, Kim, 1. (1961). Studies on human serum §-lipoproteins, including their protein moiety. (Thesis). University of Gothenburg / Göteborgs Universitet. Retrieved from http://hdl.handle.net/2077/17427

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Cramér, Kim, 1924-1992. “Studies on human serum §-lipoproteins, including their protein moiety.” 1961. Thesis, University of Gothenburg / Göteborgs Universitet. Accessed January 22, 2021. http://hdl.handle.net/2077/17427.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Cramér, Kim, 1924-1992. “Studies on human serum §-lipoproteins, including their protein moiety.” 1961. Web. 22 Jan 2021.

Vancouver:

Cramér, Kim 1. Studies on human serum §-lipoproteins, including their protein moiety. [Internet] [Thesis]. University of Gothenburg / Göteborgs Universitet; 1961. [cited 2021 Jan 22]. Available from: http://hdl.handle.net/2077/17427.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Cramér, Kim 1. Studies on human serum §-lipoproteins, including their protein moiety. [Thesis]. University of Gothenburg / Göteborgs Universitet; 1961. Available from: http://hdl.handle.net/2077/17427

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Canterbury

7. Burgess, Laura Margaret. Cytotoxicity of Hypochlorite-oxidised Proteins.

Degree: MS, Biochemistry, 2012, University of Canterbury

URL: http://dx.doi.org/10.26021/7726

► The role of cell death in atherosclerosis remains ill-defined, however, a growing body of evidence suggests that cell death stimulates atherogenesis through the induction of… (more)

▼ The role of cell death in atherosclerosis remains ill-defined, however, a growing body of evidence suggests that cell death stimulates atherogenesis through the induction of inflammation and enlargement of the necrotic core. Although there is solid evidence to suggest that lipid oxidation and toxicity are linked, indications that protein oxidation may play an important role in cytotoxicity are numerous. The abundance of dead cells in atherosclerotic plaques and their co-localization with HOCl-modified proteins provides an opening for the suggestion that the products of protein oxidation may be at the heart of oxLDL-induced cell death. Examination of the modification of LDL and albumin by HOCl, and the cytotoxicity of these oxidised molecules were the focus of this study, along with the elucidation of their cell death mechanisms toward U937 cells. Measurement of lipid peroxidation markers, TBARS and 7-ketocholesterol, showed no significant increase in HOCl-oxLDL compared to native levels although all α-tocopherol had been lost. In contrast there was a large loss of tyrosine, of which a small percentage went to dityrosine, indicating that the protein moiety of LDL was the main target of HOCl attack. Albumin became fragmented and smeared on SDS-PAGE gels with increasing HOCl/BSA molar ratios. In addition there was significant reduction in tyrosine levels and a small increase in dityrosine. Both HOCl-oxLDL and oxidised albumin (oxALB) caused concentration-dependent cell viability loss in U937 cells following a significant drop in intracellular GSH concentration, coinciding with a peak in oxidative stress. Removal of chloramines with methionine significantly reduced the toxicity of oxALB, but at higher concentrations this effect was reduced. This was in contrast to HOCl-oxLDL where the removal of chloramines had no effect on its toxicity. Morphological observations of cell swelling, cell membrane integrity loss and rupture, along with flow cytometry results indicate that U937 cells underwent necrosis, but only after intracellular GSH was lost. Intracellular GSH and cell viability loss were prevented by 200 μM extracellular 7,8-dihydroneopterin (78NP), indicating that 78NP scavenging of ROS generated in response to the oxidised proteins was sufficient to prevent cell death. This study demonstrates the cytotoxicity of HOCl-damaged LDL and albumin is likely due to a common oxidative product or structural motif which may be active within atherosclerotic plaques.

Subjects/Keywords: atherosclerosis; oxidised-proteins; LDL

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Burgess, L. M. (2012). Cytotoxicity of Hypochlorite-oxidised Proteins. (Masters Thesis). University of Canterbury. Retrieved from http://dx.doi.org/10.26021/7726

Chicago Manual of Style (16^th Edition):

Burgess, Laura Margaret. “Cytotoxicity of Hypochlorite-oxidised Proteins.” 2012. Masters Thesis, University of Canterbury. Accessed January 22, 2021. http://dx.doi.org/10.26021/7726.

MLA Handbook (7^th Edition):

Burgess, Laura Margaret. “Cytotoxicity of Hypochlorite-oxidised Proteins.” 2012. Web. 22 Jan 2021.

Vancouver:

Burgess LM. Cytotoxicity of Hypochlorite-oxidised Proteins. [Internet] [Masters thesis]. University of Canterbury; 2012. [cited 2021 Jan 22]. Available from: http://dx.doi.org/10.26021/7726.

Council of Science Editors:

Burgess LM. Cytotoxicity of Hypochlorite-oxidised Proteins. [Masters Thesis]. University of Canterbury; 2012. Available from: http://dx.doi.org/10.26021/7726

University of Manitoba

8. Sangle, Ganesh. Mechanisms for Oxidized or Glycated LDL-induced Oxidative Stress and Upregulation of Plasminogen Activator Inhibitor-1 in Vascular Cells.

Degree: Physiology, 2010, University of Manitoba

URL: http://hdl.handle.net/1993/4185

► Atherosclerotic cardiovascular disease is the leading cause of death of adults in North America. Diabetes is a classical risk factor for atherosclerotic cardiovascular disease. Plasminogen… (more)

▼ Atherosclerotic cardiovascular disease is the leading cause of death of adults in North America. Diabetes is a classical risk factor for atherosclerotic cardiovascular disease. Plasminogen activator inhibitor-1 (PAI-1) is the major physiological inhibitor of fibrinolysis. Elevated levels of PAI-1, oxidized low-density lipoprotein (oxLDL) and glycated LDL (glyLDL) were detected in patients with diabetes. Increased oxidative stress is associated with diabetic cardiovascular complications. Previous studies in our laboratory demonstrated that oxLDL or glyLDL increased the production of PAI-1 or reactive oxygen species (ROS) in vascular endothelial cells (EC). This study was undertaken to investigate transmembrane signaling mechanisms involved in oxLDL or glyLDL-induced upregulation of PAI-1 in cultured vascular EC. Further, we examined the mechanism for oxLDL or glyLDL-induced oxidative stress in EC. The results of the present studies demonstrated novel transmembrane signaling pathway for oxLDL-induced PAI-1 production in vascular EC. We demonstrated that lectin-like oxLDL receptor-1, H-Ras, a small G-protein and Raf-1/ERK-1/2 mediate oxLDL-induced PAI-1 expression in cultured EC. GlyLDL may activate EC via a distinct transmembrane signaling pathway. The results of the present study demonstrated that receptor for advanced glycation end products, NADPH oxidase and H-Ras/Raf-1 are implicated in the upregulation of heat shock factor-1 or PAI-1 in vascular EC under diabetes-associated metabolic stress. We investigated the effects of oxLDL or glyLDL on mitochondrial function in EC. Treatment with oxLDL or glyLDL significantly impaired the activities of electron transport chain (ETC) enzymes and also increased mitochondria-associated ROS in EC. The findings suggest that oxLDL or glyLDL attenuated activity of ETC and increased ROS generation in EC, which potentially contributes to oxidative stress in vasculature. In conclusion, diabetes-associated lipoproteins may upregulate stress response mediators and PAI-1 production via distinct transmembrane signaling pathways. OxLDL or glyLDL may increase ROS production via NOX activation and the impairment of mitochondrial ETC enzyme activity in EC. The understanding and identification of the regulatory mechanisms involved in diabetes-associated lipoprotein-induced signaling may help pharmacological design for the management of diabetic cardiovascular complications. Advisors/Committee Members: Shen, Garry (Internal Medicine) (supervisor), Halayko, Andrew (Physiology) Nyomba, Gregoire (Internal Medicine) Moghadasian, Mohammed (Human Nutritional Sciences) Chakrabarti, Subrata (University of Western Ontario) (examiningcommittee).

Subjects/Keywords: Plasminogen activator inhibitor-1; Atherosclerosis; glycated LDL; oxidized LDL; diabetes

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Sangle, G. (2010). Mechanisms for Oxidized or Glycated LDL-induced Oxidative Stress and Upregulation of Plasminogen Activator Inhibitor-1 in Vascular Cells. (Thesis). University of Manitoba. Retrieved from http://hdl.handle.net/1993/4185

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Sangle, Ganesh. “Mechanisms for Oxidized or Glycated LDL-induced Oxidative Stress and Upregulation of Plasminogen Activator Inhibitor-1 in Vascular Cells.” 2010. Thesis, University of Manitoba. Accessed January 22, 2021. http://hdl.handle.net/1993/4185.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Sangle, Ganesh. “Mechanisms for Oxidized or Glycated LDL-induced Oxidative Stress and Upregulation of Plasminogen Activator Inhibitor-1 in Vascular Cells.” 2010. Web. 22 Jan 2021.

Vancouver:

Sangle G. Mechanisms for Oxidized or Glycated LDL-induced Oxidative Stress and Upregulation of Plasminogen Activator Inhibitor-1 in Vascular Cells. [Internet] [Thesis]. University of Manitoba; 2010. [cited 2021 Jan 22]. Available from: http://hdl.handle.net/1993/4185.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Sangle G. Mechanisms for Oxidized or Glycated LDL-induced Oxidative Stress and Upregulation of Plasminogen Activator Inhibitor-1 in Vascular Cells. [Thesis]. University of Manitoba; 2010. Available from: http://hdl.handle.net/1993/4185

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Universidade do Rio Grande do Sul

9. Cammerer, Magda Ambros. Efeitos de uma dieta rica em flavonoides sobre o estresse oxidativo, inflamação e perfil lipídico em pacientes submetidos à angioplastia coronária com implante de stent : ensaio clínico randomizado.

Degree: 2012, Universidade do Rio Grande do Sul

URL: http://hdl.handle.net/10183/70395

► Introdução: A ingestão de alimentos antioxidantes pode reduzir o estresse oxidativo e contribuir de forma direta no processo aterosclerótico através da redução do LDL oxidado.… (more)

Subjects/Keywords: Flavonoides; Estresse oxidativo; LDL-colesterol; Angioplastia

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Cammerer, M. A. (2012). Efeitos de uma dieta rica em flavonoides sobre o estresse oxidativo, inflamação e perfil lipídico em pacientes submetidos à angioplastia coronária com implante de stent : ensaio clínico randomizado. (Thesis). Universidade do Rio Grande do Sul. Retrieved from http://hdl.handle.net/10183/70395

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Cammerer, Magda Ambros. “Efeitos de uma dieta rica em flavonoides sobre o estresse oxidativo, inflamação e perfil lipídico em pacientes submetidos à angioplastia coronária com implante de stent : ensaio clínico randomizado.” 2012. Thesis, Universidade do Rio Grande do Sul. Accessed January 22, 2021. http://hdl.handle.net/10183/70395.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Cammerer, Magda Ambros. “Efeitos de uma dieta rica em flavonoides sobre o estresse oxidativo, inflamação e perfil lipídico em pacientes submetidos à angioplastia coronária com implante de stent : ensaio clínico randomizado.” 2012. Web. 22 Jan 2021.

Vancouver:

Cammerer MA. Efeitos de uma dieta rica em flavonoides sobre o estresse oxidativo, inflamação e perfil lipídico em pacientes submetidos à angioplastia coronária com implante de stent : ensaio clínico randomizado. [Internet] [Thesis]. Universidade do Rio Grande do Sul; 2012. [cited 2021 Jan 22]. Available from: http://hdl.handle.net/10183/70395.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Cammerer MA. Efeitos de uma dieta rica em flavonoides sobre o estresse oxidativo, inflamação e perfil lipídico em pacientes submetidos à angioplastia coronária com implante de stent : ensaio clínico randomizado. [Thesis]. Universidade do Rio Grande do Sul; 2012. Available from: http://hdl.handle.net/10183/70395

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

10. Miyazawa, Haruna. Increased serum PCSK9 concentrations are associated with periodontal infection but do not correlate with LDL cholesterol concentration : 血清PCSK9レベルの上昇は歯周炎罹患と関連するが、LDLコレステロールレベルとは相関しない.

Degree: 博士（歯学）, 2014, Niigata University / 新潟大学

URL: http://hdl.handle.net/10191/27391

学位の種類: 博士（歯学）. 報告番号: 甲第3878号. 学位記番号: 新大院博（歯）甲第297号. 学位授与年月日: 平成26年3月24日

Clinica Chimica Acta. 2012, 413(1-2), 154-159

Subjects/Keywords: PCSK9; LDL cholesterol; Periodontitis; Chronic inflammation

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Miyazawa, H. (2014). Increased serum PCSK9 concentrations are associated with periodontal infection but do not correlate with LDL cholesterol concentration : 血清PCSK9レベルの上昇は歯周炎罹患と関連するが、LDLコレステロールレベルとは相関しない. (Thesis). Niigata University / 新潟大学. Retrieved from http://hdl.handle.net/10191/27391

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Miyazawa, Haruna. “Increased serum PCSK9 concentrations are associated with periodontal infection but do not correlate with LDL cholesterol concentration : 血清PCSK9レベルの上昇は歯周炎罹患と関連するが、LDLコレステロールレベルとは相関しない.” 2014. Thesis, Niigata University / 新潟大学. Accessed January 22, 2021. http://hdl.handle.net/10191/27391.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Miyazawa, Haruna. “Increased serum PCSK9 concentrations are associated with periodontal infection but do not correlate with LDL cholesterol concentration : 血清PCSK9レベルの上昇は歯周炎罹患と関連するが、LDLコレステロールレベルとは相関しない.” 2014. Web. 22 Jan 2021.

Vancouver:

Miyazawa H. Increased serum PCSK9 concentrations are associated with periodontal infection but do not correlate with LDL cholesterol concentration : 血清PCSK9レベルの上昇は歯周炎罹患と関連するが、LDLコレステロールレベルとは相関しない. [Internet] [Thesis]. Niigata University / 新潟大学; 2014. [cited 2021 Jan 22]. Available from: http://hdl.handle.net/10191/27391.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Miyazawa H. Increased serum PCSK9 concentrations are associated with periodontal infection but do not correlate with LDL cholesterol concentration : 血清PCSK9レベルの上昇は歯周炎罹患と関連するが、LDLコレステロールレベルとは相関しない. [Thesis]. Niigata University / 新潟大学; 2014. Available from: http://hdl.handle.net/10191/27391

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Texas A&M University

11. Kieffer, Adam John. Lipoprotein Density Distributions Following Diet and Exercise Interventions.

Degree: PhD, Nutrition, 2016, Texas A&M University

URL: http://hdl.handle.net/1969.1/187338

► Atherosclerotic-related cardiovascular disease (ASCVD) claims the lives of over 600,000 Americans yearly. Current methodologies of assessment do not distinguish lipoprotein density distributions and instead measure… (more)

▼ Atherosclerotic-related cardiovascular disease (ASCVD) claims the lives of over 600,000 Americans yearly. Current methodologies of assessment do not distinguish lipoprotein density distributions and instead measure lipoprotein cholesterol, with lowdensity lipoprotein cholesterol (LDL-C) and high-density lipoprotein cholesterol (HDLC) of primary clinical relevance. Furthermore, the distinction between the effects of diet and exercise interventions on lipoproteins is frequently indiscernible due to the effects of energy deficit induced by interventions. High- performance lipoprotein density profiling (HPLDP) provides a cost-effective way to rapidly assess the efficacy of diet or exercise interventions. The purpose of this study is to characterize density distribution of HDL subclasses in response to diet or exercise using HPLDP. Eighty-eight untrained subjects (74% male, average age of 54 years) were pooled from two lifestyle intervention studies that met the inclusion criteria. Following 12 weeks of diet or exercise interventions (750 kcal expenditure or 750 kcal deficit), subjects lost an average of 3.72 kg weight (-3.9%), lost 3.9 kg of body fat (-11.6%), increased lean mass 0.62 kg (+1.1%), and reduced body fat percentage by 3.41% (-9.1%). Average absolute VO2 max increased 0.16 liters O2/min (+7.1%) (p<0.05). Several lipoprotein density distributions were significantly different (p<0.05) between diet and exercise interventions (expressed as percent change from baseline): triglyceride-rich lipoproteins (TRL) (-17.14 vs 19.65), low-density lipoprotein subfraction 5 (LDL5) (-26.06 vs 8.14), high-density lipoprotein subfraction 3b (HDL3b) (-21.24 vs -0.71), and high-density lipoprotein subfraction 3c (HDL3c) (-17.88 vs 7.94). To elucidate the effects of cardiorespiratory fitness, subjects were further divided into categories of increased absolute VO2 max and decreased absolute VO2 max, with no changes between groups at baseline. Associations between absolute VO2 max percent change on TRL, LDL-5, HDL-3b, and HDL-3c percent change remained significant after controlling for age, gender, and fat mass percent change. A regression equation was constructed from significant correlations and effectively predicted HDL-3c changes using absolute VO2 max measurements. A significant linear relationship between improved absolute VO2 max and increased HDL-3c subfraction AUC exists; lipoprotein subfraction quantification may reveal positive effects of exercise overlooked using traditional clinical cholesterol assessment techniques. Advisors/Committee Members: Walzem, Rosemary L (advisor), Crouse, Stephen F (committee member), Riechman, Steven E (committee member), Smith, Stephen B (committee member).

Subjects/Keywords: lipoproteins; lipid; HDL; LDL; exercise; diet

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Kieffer, A. J. (2016). Lipoprotein Density Distributions Following Diet and Exercise Interventions. (Doctoral Dissertation). Texas A&M University. Retrieved from http://hdl.handle.net/1969.1/187338

Chicago Manual of Style (16^th Edition):

Kieffer, Adam John. “Lipoprotein Density Distributions Following Diet and Exercise Interventions.” 2016. Doctoral Dissertation, Texas A&M University. Accessed January 22, 2021. http://hdl.handle.net/1969.1/187338.

MLA Handbook (7^th Edition):

Kieffer, Adam John. “Lipoprotein Density Distributions Following Diet and Exercise Interventions.” 2016. Web. 22 Jan 2021.

Vancouver:

Kieffer AJ. Lipoprotein Density Distributions Following Diet and Exercise Interventions. [Internet] [Doctoral dissertation]. Texas A&M University; 2016. [cited 2021 Jan 22]. Available from: http://hdl.handle.net/1969.1/187338.

Council of Science Editors:

Kieffer AJ. Lipoprotein Density Distributions Following Diet and Exercise Interventions. [Doctoral Dissertation]. Texas A&M University; 2016. Available from: http://hdl.handle.net/1969.1/187338

University of Vienna

12. Gensberger, Eva Theres. The role of low-density-lipoprotein receptor (LDLR) family members in human coronary artery endothelial cells in response to modified LDL.

Degree: 2010, University of Vienna

URL: http://othes.univie.ac.at/11804/

► Erste Anzeichen der Atherosklerose kennzeichnen sich durch die endotheliale Dysfunktion in Folge von massiver Schädigung des Endothels in der Intima der Blutgefäße. Der Blutfluss und… (more)

▼ Erste Anzeichen der Atherosklerose kennzeichnen sich durch die endotheliale Dysfunktion in Folge von massiver Schädigung des Endothels in der Intima der Blutgefäße. Der Blutfluss und die dabei wirkenden mechanischen Scherkräfte spielen eine entscheidende Rolle in der Gefäßhomeostase und der Aufrechterhaltung der Endothelfunktion. An Abgängen, Verzweigungen und Verengungen von Blutgefäßen wirken geringere Scherkräfte und der Blutfluss verlangsamt sich, infolgedessen treten an diesen Stellen vermehrt atherosklerotische Plauqes auf. Verschiedene Krankheiten wie Diabetes, chronische Niereninsuffizienz und Übergewicht gehen mit erhöhtem Blutcholesterolspiegel einher. Patienten haben durch die Krankheit vermehrt modifizierte Lipoproteine im Blut. Modifikationsprozesse von LDL stehen mit ersten Anzeichen der Atherosklerose in Verbindung. Humane koronare arterielle Endothelzellen (HCAEC) werden häufig im Forschungsbereich der Atherosklerose, insbesondere der endothelialen Dysfunktion eingesetzt, um die Wirkung von modifizierten LDLs und die dabei resultierende Schädigung der Endothelzellen herauszufinden. HDL und das weibliche Hormon Östrogen wirken Schädigungen entgegen und werden als vasoprotektiv beschrieben. Anhand von Toxizitätstests konnte die Lipotoxizität mit steigender Konzentration an modifizierten LDL auf Endothelzellen gezeigt werden. Die Vermutung konnte bekräftigt werden, dass HDL und 17-beta Estradiol (E2) den toxischen Effekt in Zellkulturexperimenten vermindern konnten. Frühere Studien befassten sich mit der Regulation von inflammatorischen Markern und der Regulierung von Scavenger Rezeptoren, den sogenannten Fressrezeptoren. Bisher ist wenig über die Regulation von LDL Rezeptor Familienmitgliedern im vaskulären Endothelium bekannt. Das Ziel meiner Diplomarbeit war, die Rolle von LDL Rezeptor Familienmitgliedern, wie LDL Rezeptor, LRP1 und LRP2, in diesem Zusammenhang zu definieren. Hierbei sollten HCAEC mit verschiedenen modifizierten LDLs (oxidiertem, glykiertem und carbamyliertem LDL) behandelt werden. Weiters sollten geschlechtsspezifische Unterschiede erfasst werden, indem Zellen zusätzlich zu modifizierten LDL mit E2 behandelt wurden. Die Expressionsanalysen wurden unter statischen Bedingungen und unter Einwirkung von Scherkräften in Zellkartuschen (Fiber Cell™ Model) durchgeführt. Zusammenfassend konnte gezeigt werden, dass der Transkriptionslevel von LDL Rezeptoren unter statischen Bedingungen und unter geringen Scherkräften verringert wird. Erhöhter Blutfluss und Scherkräfte wirken aktiv auf Rezeptoren der Endothelzellen. LRP2 mRNA konnte nur mit geringem Expressionstatus nachgewiesen werden. LRP1 konnte auf Transkriptionsebene in Zellkulturexperimenten nicht nachgewiesen werden, aber eine basale Aktivierung unter Einfluss von Scherkräften. In Zellkulturexperimenten zeigte die Inkubation von Endothelzellen mit E2 und modifizierten LDL, einen geringen Antsieg von LRP1 und LRP2 auf mRNA Ebene. Eine…

Subjects/Keywords: 42.13 Molekularbiologie; Atherosklerose / endotheliale Dysfunktion / modifiziertes LDL / LDL Rezeptor Familie / Toxizität / Östrogen; Atherosclerosis / endothelial dysfunction / modified LDL, LDL receptor family / toxicity / estrogen

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Gensberger, E. T. (2010). The role of low-density-lipoprotein receptor (LDLR) family members in human coronary artery endothelial cells in response to modified LDL. (Thesis). University of Vienna. Retrieved from http://othes.univie.ac.at/11804/

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Gensberger, Eva Theres. “The role of low-density-lipoprotein receptor (LDLR) family members in human coronary artery endothelial cells in response to modified LDL.” 2010. Thesis, University of Vienna. Accessed January 22, 2021. http://othes.univie.ac.at/11804/.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Gensberger, Eva Theres. “The role of low-density-lipoprotein receptor (LDLR) family members in human coronary artery endothelial cells in response to modified LDL.” 2010. Web. 22 Jan 2021.

Vancouver:

Gensberger ET. The role of low-density-lipoprotein receptor (LDLR) family members in human coronary artery endothelial cells in response to modified LDL. [Internet] [Thesis]. University of Vienna; 2010. [cited 2021 Jan 22]. Available from: http://othes.univie.ac.at/11804/.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Gensberger ET. The role of low-density-lipoprotein receptor (LDLR) family members in human coronary artery endothelial cells in response to modified LDL. [Thesis]. University of Vienna; 2010. Available from: http://othes.univie.ac.at/11804/

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

13. Bonneville, Jonathan. Effets athéroprotecteurs de la curcumine et d’extraits riches en polyphénols d’Antirhea borbonica et de Doratoxylon apetalum : Atheroprotective effects of curcumin and polyphenol rich extracts of Antirhea borbonica and Doratoxylon apetalum.

Degree: Docteur es, Biologie, 2018, Université de la Réunion

URL: http://www.theses.fr/2018LARE0013

►

Les maladies cardiovasculaires représentent la première cause de mortalité en France avec une prévalence encore plus importante à La Réunion. La plupart des décès sont… (more)

▼

Les maladies cardiovasculaires représentent la première cause de mortalité en France avec une prévalence encore plus importante à La Réunion. La plupart des décès sont attribuables aux cardiopathies ischémiques due à la rupture d'une plaque d'athérosclérose et à la formation d'un caillot entrainant une ischémie cardiaque. L'oxydation des lipoprotéines de basse densité (LDL) et la dysfonction de l'endothélium représentent des étapes importantes dans la déstabilisation de la plaque, d'où l’intérêt de rechercher un traitement capable de diminuer l'oxydation des LDL ou de protéger les cellules endothéliales de leurs effets cytotoxiques. Les polyphénols sont des molécules antioxydantes très présentes dans le règne végétal. Des études préliminaires du laboratoire sur des adipocytes humain on montré que des extraits riches en polyphénols d'Antirhea borbonica et de Doratoxylon apetalum, deux espèces respectivement endémique et indigène de l'Île de La Réunion, possédaient des propriétés antioxydantes et anti-inflammatoires (Marimoutou, M et al. 2015). Le But de cette thèse était, dans un premier temps, de tester les capacités antioxydantes et cytoprotectrices d'extraits riches en polyphénols de ces deux espèces sur des cellules endothéliales. Puis dans un second temps nous avons testé l'extrait de Doratoxylon apetalum sur un modèle murin d'athérosclérose (souris ApoE KO) pour évaluer son effet anti-inflammatoire sur les plaques d'athérome.

Cardiovascular diseases are the leading cause of death in France with an even higher prevalence in Reunion. Most deaths are due to ischemic heart disease because of atherosclerotic plaque rupture and thrombus formation leading to cardiac ischemia. The oxidation of low density lipoproteins (LDL) and endothelial dysfunction represent important steps in the destabilization of plaque, hence the interest of a treatment capable of reducing the oxidation of LDL or of protecting endothelial cells from their cytotoxic effects. Polyphenols are antioxidant molecules very present in the plant kingdom. Preliminary studies on human adipocytes have shown that polyphenol-rich extracts of Antirhea borbonica and Doratoxylon apetalum, respectively endemic and indigenous species of Reunion Island, had antioxidant and anti-inflammatory properties (Marimoutou , M et al., 2015). The aim of this thesis was, first, to test the antioxidant and cytoprotective capacities of polyphenol rich extracts of these two species on endothelial cells. Then in a second time we tested the extract of Doratoxylon apetalum on a murine model of atherosclerosis (ApoE KO mice) to evaluate its anti-inflammatory effect on atheroma plaques.

Advisors/Committee Members: Vindis, Cécile (thesis director), Meilhac, Olivier (thesis director).

Subjects/Keywords: Polyphénols; Athérosclérose; LDL; LDL oxydées; Doratoxylon apetalum; Antirhea Borbonica; Antioxydant; Cytoprotecteur; Polyphenols; Atherosclerosis; LDL; Oxidised LDL; Doratoxylon apetalum; Antirhea Borbonica; Antioxidant; Cytoprotective

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Bonneville, J. (2018). Effets athéroprotecteurs de la curcumine et d’extraits riches en polyphénols d’Antirhea borbonica et de Doratoxylon apetalum : Atheroprotective effects of curcumin and polyphenol rich extracts of Antirhea borbonica and Doratoxylon apetalum. (Doctoral Dissertation). Université de la Réunion. Retrieved from http://www.theses.fr/2018LARE0013

Chicago Manual of Style (16^th Edition):

Bonneville, Jonathan. “Effets athéroprotecteurs de la curcumine et d’extraits riches en polyphénols d’Antirhea borbonica et de Doratoxylon apetalum : Atheroprotective effects of curcumin and polyphenol rich extracts of Antirhea borbonica and Doratoxylon apetalum.” 2018. Doctoral Dissertation, Université de la Réunion. Accessed January 22, 2021. http://www.theses.fr/2018LARE0013.

MLA Handbook (7^th Edition):

Bonneville, Jonathan. “Effets athéroprotecteurs de la curcumine et d’extraits riches en polyphénols d’Antirhea borbonica et de Doratoxylon apetalum : Atheroprotective effects of curcumin and polyphenol rich extracts of Antirhea borbonica and Doratoxylon apetalum.” 2018. Web. 22 Jan 2021.

Vancouver:

Bonneville J. Effets athéroprotecteurs de la curcumine et d’extraits riches en polyphénols d’Antirhea borbonica et de Doratoxylon apetalum : Atheroprotective effects of curcumin and polyphenol rich extracts of Antirhea borbonica and Doratoxylon apetalum. [Internet] [Doctoral dissertation]. Université de la Réunion; 2018. [cited 2021 Jan 22]. Available from: http://www.theses.fr/2018LARE0013.

Council of Science Editors:

Bonneville J. Effets athéroprotecteurs de la curcumine et d’extraits riches en polyphénols d’Antirhea borbonica et de Doratoxylon apetalum : Atheroprotective effects of curcumin and polyphenol rich extracts of Antirhea borbonica and Doratoxylon apetalum. [Doctoral Dissertation]. Université de la Réunion; 2018. Available from: http://www.theses.fr/2018LARE0013

University of Debrecen

14. Bálint, Emőke. Low-density lipoprotein és high-density lipoprotein szubfrakciók elválasztása gélelektroforézissel .

Degree: DE – Általános Orvostudományi Kar, University of Debrecen

URL: http://hdl.handle.net/2437/241550

► Bevezetés: Az LDL és HDL lipoproteinek fontos szerepet töltenek be az érelmeszesedés folyamatában. Ezen partikulumok nemcsak koleszterintartalmuk alapján jellemezhetők, hanem fizikai és kémiai tulajdonságukban, apolipoprotein… (more)

▼ Bevezetés: Az LDL és HDL lipoproteinek fontos szerepet töltenek be az érelmeszesedés folyamatában. Ezen partikulumok nemcsak koleszterintartalmuk alapján jellemezhetők, hanem fizikai és kémiai tulajdonságukban, apolipoprotein és lipid tartalmukban, élettani szerepükben is különbözőek, heterogén szubfrakciókra oszthatók. Heterogenitásuk jellemzésére többféle analitikai módszer áll rendelkezésre. Célkitűzés: Diplomamunkámban célul tűztem ki különböző szérum minták stabilitásának vizsgálatát alacsony hőmérsékleten történő, hosszú távú mintatárolás során, valamint különböző kardiovaszkuláris megbetegedésben szenvedő betegek lipoprotein szubfrakciós profiljának elemzését és egészséges személyek adataival való összehasonlítását. Módszerek: A szérum minták lipoprotein szubfrakcióinak elkülönítése méretük alapján üvegcsöves akrilamid gélelektroforézissel, Lipoprint® rendszerrel történt. Az LDL teszt esetében 7 különböző LDL szubfrakciót választunk el. HDL teszt esetében 10 különböző HDL szubfrakció elválasztására van lehetőségünk. Eredmények: 4 személytől vettünk mintát és a szérum mintából elválasztott lipoprotein szubfrakciók mintastabilitását vizsgáltuk a 0., 7. és 30. napon -70°C-on tárolt mintákból. LDL szubfrakciók elválasztása során az egyes sávok között 0-3,2% közötti eltérést, a HDL szubfrakciók elválasztása esetében 0,08-2,33% közötti eltérést detektáltunk a 0. napon kapott eredményekhez képest. Vizsgálataink második felében egészséges személyek lipoprotein szubfrakciós profilját vetettük össze elhízott, ill. frissen diagnosztizált hiperlipidémiás személyek adataival, amely során a betegekben a kis, denz LDL szubfrakció, ill. a kisméretű HDL szubfrakció nagyobb arányú jelenlétét figyeltük meg az egészséges személyekhez viszonyítva. Következtetések: 30 napig történő, alacsony hőmérsékleten való mintatárolás során a szérumban nem léptek fel a lipoprotein szubfrakciós profil megváltozását előidéző érdemi változások. Az adatok pontosítása érdekében a méréseket érdemes kiterjeszteni hosszabb ideig tárolt mintákra is. A betegek szubfrakciós profiljában megfigyelhető eltérések fontos információkat szolgáltatnak a későbbi kardiovaszkuláris komplikációk és az érelmeszesedés folyamatának jellemzésére. Advisors/Committee Members: Lőrincz, Hajnalka (advisor), Debreceni Egyetem::Általános Orvostudományi Kar::Belgyógyászati Intézet (advisor).

Subjects/Keywords: LDL; HDL; lipoprotein

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Bálint, E. (n.d.). Low-density lipoprotein és high-density lipoprotein szubfrakciók elválasztása gélelektroforézissel . (Thesis). University of Debrecen. Retrieved from http://hdl.handle.net/2437/241550

Note: this citation may be lacking information needed for this citation format:
No year of publication.
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Bálint, Emőke. “Low-density lipoprotein és high-density lipoprotein szubfrakciók elválasztása gélelektroforézissel .” Thesis, University of Debrecen. Accessed January 22, 2021. http://hdl.handle.net/2437/241550.

Note: this citation may be lacking information needed for this citation format:
No year of publication.
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Bálint, Emőke. “Low-density lipoprotein és high-density lipoprotein szubfrakciók elválasztása gélelektroforézissel .” Web. 22 Jan 2021.

Note: this citation may be lacking information needed for this citation format:
No year of publication.

Vancouver:

Bálint E. Low-density lipoprotein és high-density lipoprotein szubfrakciók elválasztása gélelektroforézissel . [Internet] [Thesis]. University of Debrecen; [cited 2021 Jan 22]. Available from: http://hdl.handle.net/2437/241550.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
No year of publication.

Council of Science Editors:

Bálint E. Low-density lipoprotein és high-density lipoprotein szubfrakciók elválasztása gélelektroforézissel . [Thesis]. University of Debrecen; Available from: http://hdl.handle.net/2437/241550

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
No year of publication.

University of Toronto

15. Sugiyama, Michael Glen. Elucidation of the Mechanisms of Transcellular and Paracellular Endothelial Permeability – Towards its Therapeutic Manipulation.

Degree: PhD, 2018, University of Toronto

URL: http://hdl.handle.net/1807/82954

► Every blood vessel in the body is lined on its luminal aspect with a single layer of interconnected cells, known as endothelial cells. Together, these… (more)

▼ Every blood vessel in the body is lined on its luminal aspect with a single layer of interconnected cells, known as endothelial cells. Together, these cells form a tissue known as the endothelium, a tightly regulated system that physically segregates the fluid in the blood from the peripheral tissues, and orchestrates the passage of nutrients, xenobiotics, immune cells, and fluids between distinct compartments in the body. In healthy, quiescent vasculature, passage of compounds across the vasculature occurs by two separate, yet equally important mechanisms. In the paracellular route, compounds that are small enough to pass between endothelial cell junctions freely diffuse down their concentration gradient, reaching equilibrium between the blood and sub-endothelial compartments. In contrast, transcytosis involves the regulated transit of compounds through endothelial cells by endocytosis, subcellular trafficking, and exocytosis, and in doing so, allows for the transport of larger compounds through the use of a number of specialized proteins. Under normal circumstances, these two mechanisms of endothelial permeability work in concert to maintain homeostasis. However it has recently become apparent that dysregulated endothelial permeability is the cause of a number of pathological conditions. Increased paracellular permeability of the lung endothelium leads to the acute respiratory distress syndrome (ARDS), a potentially fatal condition involving acute lung edema, respiratory failure, and death. Endothelial transcytosis of low-density lipoprotein (LDL) leads to the accumulation of LDL in the subendothelial intima, the initiating event in atherosclerotic lesion development. Although dysregulated endothelial permeability is central to the pathophysiology of these diseases, the paucity of literature on the subject highlights the need for a detailed, mechanistic investigation. In this thesis I present a body of work that uncovers several novel mechanisms that regulate endothelial permeability in the pathological contexts of pathogen-induced ARDS and LDL transcytosis as a precursor to atherosclerotic lesion development. Results from these studies suggest that targeting endothelial permeability is novel and viable therapeutic strategy for management of these common diseases. Advisors/Committee Members: Lee, Warren L, Laboratory Medicine and Pathobiology.

Subjects/Keywords: Atherosclerosis; Endothelium; Inflammation; Influenza; LDL; Permeability; 0379

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APA (6^th Edition):

Sugiyama, M. G. (2018). Elucidation of the Mechanisms of Transcellular and Paracellular Endothelial Permeability – Towards its Therapeutic Manipulation. (Doctoral Dissertation). University of Toronto. Retrieved from http://hdl.handle.net/1807/82954

Chicago Manual of Style (16^th Edition):

Sugiyama, Michael Glen. “Elucidation of the Mechanisms of Transcellular and Paracellular Endothelial Permeability – Towards its Therapeutic Manipulation.” 2018. Doctoral Dissertation, University of Toronto. Accessed January 22, 2021. http://hdl.handle.net/1807/82954.

MLA Handbook (7^th Edition):

Sugiyama, Michael Glen. “Elucidation of the Mechanisms of Transcellular and Paracellular Endothelial Permeability – Towards its Therapeutic Manipulation.” 2018. Web. 22 Jan 2021.

Vancouver:

Sugiyama MG. Elucidation of the Mechanisms of Transcellular and Paracellular Endothelial Permeability – Towards its Therapeutic Manipulation. [Internet] [Doctoral dissertation]. University of Toronto; 2018. [cited 2021 Jan 22]. Available from: http://hdl.handle.net/1807/82954.

Council of Science Editors:

Sugiyama MG. Elucidation of the Mechanisms of Transcellular and Paracellular Endothelial Permeability – Towards its Therapeutic Manipulation. [Doctoral Dissertation]. University of Toronto; 2018. Available from: http://hdl.handle.net/1807/82954

University of Arizona

16. Hunjan, Anoop Singh. Chronic Arsenite Exposure in Lung Epithelium Modulates Endocytosis .

Degree: 2017, University of Arizona

URL: http://hdl.handle.net/10150/626765

► Arsenic exposure in humans has been implicated in the development of a myriad of non-cancerous and cancerous diseases. A reductionist approach to understanding this unusual… (more)

Subjects/Keywords: Albumin; Arsenic; BEAS-2B; Endocytosis; LDL; Transferrin

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Hunjan, A. S. (2017). Chronic Arsenite Exposure in Lung Epithelium Modulates Endocytosis . (Masters Thesis). University of Arizona. Retrieved from http://hdl.handle.net/10150/626765

Chicago Manual of Style (16^th Edition):

Hunjan, Anoop Singh. “Chronic Arsenite Exposure in Lung Epithelium Modulates Endocytosis .” 2017. Masters Thesis, University of Arizona. Accessed January 22, 2021. http://hdl.handle.net/10150/626765.

MLA Handbook (7^th Edition):

Hunjan, Anoop Singh. “Chronic Arsenite Exposure in Lung Epithelium Modulates Endocytosis .” 2017. Web. 22 Jan 2021.

Vancouver:

Hunjan AS. Chronic Arsenite Exposure in Lung Epithelium Modulates Endocytosis . [Internet] [Masters thesis]. University of Arizona; 2017. [cited 2021 Jan 22]. Available from: http://hdl.handle.net/10150/626765.

Council of Science Editors:

Hunjan AS. Chronic Arsenite Exposure in Lung Epithelium Modulates Endocytosis . [Masters Thesis]. University of Arizona; 2017. Available from: http://hdl.handle.net/10150/626765

University of Sydney

17. Abdo, Adrian Issa. The role of low-density lipoprotein modified by myeloperoxidasederived oxidants on vascular dysfunction in atherosclerosis .

Degree: 2017, University of Sydney

URL: http://hdl.handle.net/2123/18090

► Atherosclerosis is characterised by inflammation, arterial stiffening and aberrant lipid transport by deposition of low-density lipoproteins (LDL) in the artery wall that is susceptible to… (more)

▼ Atherosclerosis is characterised by inflammation, arterial stiffening and aberrant lipid transport by deposition of low-density lipoproteins (LDL) in the artery wall that is susceptible to oxidation. Oxidised LDL is taken up by endothelial cells, which can lead to impaired vasorelaxation. There is strong evidence for the role of myeloperoxidase (MPO), an inflammatory enzyme released by neutrophils, in oxidative damage to biomolecules such as LDL in atherosclerosis. MPO produces the oxidants HOCl and HOSCN. While it is well established that HOCl causes extensive LDL modification, less is known regarding HOSCN in atherosclerosis, which may be of relevance to smokers whom have significantly elevated plasma levels of the MPO substrate thiocyanate (SCN-), which can shift MPO to favour HOSCN production. Therefore, modifications of LDL by HOSCN may be a contributing factor to the deleterious effects of smoking in the development of atherosclerosis. This study examined modification of the LDL protein apoB-100 by HOSCN and found it selectively cleaved the N- and C-termini by free-radical mediated reaction. However, studies into the specific mechanism and determining the cleavage sites are still required. The role of HOSCN- or HOCl-modified LDL in inducing vascular cell dysfunction was also investigated. Exposing these modified LDL to endothelial cells did not induce inflammatory adhesion molecule or cytokine expression, unfolded protein response gene expression, or alter neutrophil adhesion. However, HOSCN- and HOCl-modified LDL did significantly impair nitric oxide (NO) production by uncoupling endothelial NO synthase (eNOS). This supported functional assessment of vasorelaxation by wire myography in rat aortic segments exposed to HOSCN- or HOCl-modified LDL ex vivo, which had impaired endothelium-dependent vasodilation. In cultured vascular smooth muscle cells (VSMC) exposed to these modified LDL, the gene expression of contractile markers α-smooth muscle actin and calponin were stunted, while the synthetic marker osteopontin was elevated. This is consistent with promotion of a synthetic VSMC phenotype. The data from this study support a role for HOSCN as well as HOCl in promoting vascular dysfunction by modification of LDL, which is an important mechanism in promoting atherosclerosis.

Subjects/Keywords: Endothelial dysfunction; Myeloperoxidase; LDL; eNOS; Atherosclerosis

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APA (6^th Edition):

Abdo, A. I. (2017). The role of low-density lipoprotein modified by myeloperoxidasederived oxidants on vascular dysfunction in atherosclerosis . (Thesis). University of Sydney. Retrieved from http://hdl.handle.net/2123/18090

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Abdo, Adrian Issa. “The role of low-density lipoprotein modified by myeloperoxidasederived oxidants on vascular dysfunction in atherosclerosis .” 2017. Thesis, University of Sydney. Accessed January 22, 2021. http://hdl.handle.net/2123/18090.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Abdo, Adrian Issa. “The role of low-density lipoprotein modified by myeloperoxidasederived oxidants on vascular dysfunction in atherosclerosis .” 2017. Web. 22 Jan 2021.

Vancouver:

Abdo AI. The role of low-density lipoprotein modified by myeloperoxidasederived oxidants on vascular dysfunction in atherosclerosis . [Internet] [Thesis]. University of Sydney; 2017. [cited 2021 Jan 22]. Available from: http://hdl.handle.net/2123/18090.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Abdo AI. The role of low-density lipoprotein modified by myeloperoxidasederived oxidants on vascular dysfunction in atherosclerosis . [Thesis]. University of Sydney; 2017. Available from: http://hdl.handle.net/2123/18090

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of New South Wales

18. Kazim, Abdulla. The novel role of endosomal sorting proteins in intracellular cholesterol transport.

Degree: Biotechnology & Biomolecular Sciences, 2012, University of New South Wales

URL: http://handle.unsw.edu.au/1959.4/52092 ; https://unsworks.unsw.edu.au/fapi/datastream/unsworks:10762/SOURCE01?view=true

► Cholesterol is an essential mammalian membrane component. In mammalian cells, cholesterol homeostasis needs to be tightly regulated since the aberrant intracellular cholesterol transport and storage… (more)

Subjects/Keywords: npc1; vps4; hrs; Cholesterol; ESCRT; LDL; endosome

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APA (6^th Edition):

Kazim, A. (2012). The novel role of endosomal sorting proteins in intracellular cholesterol transport. (Masters Thesis). University of New South Wales. Retrieved from http://handle.unsw.edu.au/1959.4/52092 ; https://unsworks.unsw.edu.au/fapi/datastream/unsworks:10762/SOURCE01?view=true

Chicago Manual of Style (16^th Edition):

Kazim, Abdulla. “The novel role of endosomal sorting proteins in intracellular cholesterol transport.” 2012. Masters Thesis, University of New South Wales. Accessed January 22, 2021. http://handle.unsw.edu.au/1959.4/52092 ; https://unsworks.unsw.edu.au/fapi/datastream/unsworks:10762/SOURCE01?view=true.

MLA Handbook (7^th Edition):

Kazim, Abdulla. “The novel role of endosomal sorting proteins in intracellular cholesterol transport.” 2012. Web. 22 Jan 2021.

Vancouver:

Kazim A. The novel role of endosomal sorting proteins in intracellular cholesterol transport. [Internet] [Masters thesis]. University of New South Wales; 2012. [cited 2021 Jan 22]. Available from: http://handle.unsw.edu.au/1959.4/52092 ; https://unsworks.unsw.edu.au/fapi/datastream/unsworks:10762/SOURCE01?view=true.

Council of Science Editors:

Kazim A. The novel role of endosomal sorting proteins in intracellular cholesterol transport. [Masters Thesis]. University of New South Wales; 2012. Available from: http://handle.unsw.edu.au/1959.4/52092 ; https://unsworks.unsw.edu.au/fapi/datastream/unsworks:10762/SOURCE01?view=true

Universidade do Rio Grande do Sul

19. Spessatto, Débora. Associação dos níveis de HbA1c com colesterol LDL e colesterol LDL oxidado em indivíduos não-diabéticos.

Degree: 2011, Universidade do Rio Grande do Sul

URL: http://hdl.handle.net/10183/39649

► Introdução: O Diabetes mellitus (DM) está associado a complicações crônicas micro e macrovasculares. A medida da hemoglobina glicada (HbA1c) avalia o grau do controle glicêmico… (more)

▼ Introdução: O Diabetes mellitus (DM) está associado a complicações crônicas micro e macrovasculares. A medida da hemoglobina glicada (HbA1c) avalia o grau do controle glicêmico em pacientes diabéticos e seus níveis são capazes de prognosticar o risco de desenvolvimento dessas complicações. Entre as origens das complicações causadas pela hiperglicemia, há a hipótese dos produtos finais de glicação avançada (AGEs) e do estresse oxidativo. A reação de glicação não enzimática das proteínas também está relacionada com as complicações diabéticas e é responsável pela formação da HbA1c. Entretanto, tem sido demonstrado um aumento dessa glicação em pacientes não diabéticos. Um dos prováveis mecanismos para esse aumento é a peroxidação lipídica, com conseqüente aumento nos níveis de malondialdeído (MDA) que modifica a apolipoproteína B (APO B) do colesterol de baixa densidade (LDL). A modificação oxidativa do LDL confere propriedades específicas pró-aterogênicas. A presença do LDL oxidado e o aumento da tendência do LDL à peroxidação lipídica, podem contribuir para o aumento dos níveis de HbA1c. Objetivo: Verificar a associação entre os níveis de HbA1c com o Colesterol LDL e LDL oxidado em Indivíduos não-diabéticos. Métodos: Foi realizado um estudo transversal observacional, no qual um total de 196 indivíduos, classificados como não-diabéticos, foram analisados e divididos em três grupos, conforme os valores de HbA1c e glicemia de jejum (GJ): Grupo 1 (n =64) - HbA1c <5,7% e GJ <100 mg/dL; Grupo 2 (n =69) - HbA1c ≥5,7 e ≤6,4% e GJ <100 mg/dL; Grupo 3 (n =63) - HbA1c ≥5,7 e ≤6,4% e GJ ≥100 e <126mg/dL. Amostras de sangue total e soro foram coletadas. O LDL oxidado foi medido por método imunoensaio enzimático (Mercodia ®), ApoB dosada por imunoturbidimentria, a relação Colesterol LDL(oxi)/Colesterol-HDL foi estimada, além das outras dosagens bioquímicas do perfil lipídico. Esses testes foram comparados e analisados entre os três diferentes grupos. Resultados: Houve diferença significativa nos níveis de LDL(oxi) (p< 0,001), Apo B (p= 0,026) e razão LDL(oxi)/HDL (p< 0,001) entre os três grupos. Os valores de HbA1c apresentaram correlação positiva com os valores de LDL (oxi) (r =0,431; p <0,001), LDL (r =0,148; p =0,039), Col Não-HDL (r =0,192; p =0,007) e Apo B (r =0,171; p <0,001). Estas associações positivas permaneceram significativas, mesmo após ajuste, por análise de regressão linear múltipla para as variáveis álcool, medicamentos, índice de massa corporal (IMC) e idade. Também apresentaram correlações positivas com os valores de HbA1c: razão LDL (oxi)/HDL (r =0,422; p <0,001), CT (r =0,142; p =0,048), triglicerídios (r =0,155; p =0,030) e IMC (r =0,263; p <0,001). Conclusão: Nosso estudo demonstrou associação dos níveis de HbA1c com as partículas lipídicas aterogênicas LDL, Apo B, colesterol não HDL e LDL (oxi). Os níveis de LDL, principalmente LDL (oxi), estão significativamente associados com os níveis de HbA1c e glicose, mesmo em indivíduos não-diabéticos. Os indivíduos classificados com alto risco de desenvolver DM ou… Advisors/Committee Members: Camargo, Joiza Lins.

Subjects/Keywords: HDL-colesterol; Diabetes mellitus; LDL-colesterol; Glycated hemoglobin; Oxidized cholesterol LDL; Hemoglobina A glicosilada

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Spessatto, D. (2011). Associação dos níveis de HbA1c com colesterol LDL e colesterol LDL oxidado em indivíduos não-diabéticos. (Thesis). Universidade do Rio Grande do Sul. Retrieved from http://hdl.handle.net/10183/39649

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Spessatto, Débora. “Associação dos níveis de HbA1c com colesterol LDL e colesterol LDL oxidado em indivíduos não-diabéticos.” 2011. Thesis, Universidade do Rio Grande do Sul. Accessed January 22, 2021. http://hdl.handle.net/10183/39649.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Spessatto, Débora. “Associação dos níveis de HbA1c com colesterol LDL e colesterol LDL oxidado em indivíduos não-diabéticos.” 2011. Web. 22 Jan 2021.

Vancouver:

Spessatto D. Associação dos níveis de HbA1c com colesterol LDL e colesterol LDL oxidado em indivíduos não-diabéticos. [Internet] [Thesis]. Universidade do Rio Grande do Sul; 2011. [cited 2021 Jan 22]. Available from: http://hdl.handle.net/10183/39649.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Spessatto D. Associação dos níveis de HbA1c com colesterol LDL e colesterol LDL oxidado em indivíduos não-diabéticos. [Thesis]. Universidade do Rio Grande do Sul; 2011. Available from: http://hdl.handle.net/10183/39649

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

20. Marangoni, Adriane Bueno. Baixa suplementação de azeite de oliva reduz triaciglicerois e características lipídicas e oxidativas associadas à lipoproteína de baixa densidade em indivíduos com risco cardiovascular intermediário e alto.

Degree: Mestrado, Nutrição em Saúde Pública, 2013, University of São Paulo

URL: http://www.teses.usp.br/teses/disponiveis/6/6138/tde-21102014-100904/ ;

►

Introdução: A doença cardiovascular é a principal causa de morbimortalidade precoce em todo o mundo, e responde por grande parte dos gastos dos recursos destinados… (more)

▼

Introdução: A doença cardiovascular é a principal causa de morbimortalidade precoce em todo o mundo, e responde por grande parte dos gastos dos recursos destinados aos programas de políticas públicas. Neste contexto, a dieta representa uma importante ferramenta na redução dos fatores de risco cardiovasculares. Tendo em vista que inúmeros estudos mostram que o consumo de ômega 9 ou alimento fonte modifica positivamente diversos fatores de risco cardiovascular clássicos, se torna importante avaliar seu efeito sobre propriedades físico-químicas da LDL e da HDL, marcadores cardiometabólicos e oxidativos em indivíduos brasileiros com diferentes níveis de risco cardiovascular. Objetivo: Avaliar o efeito do consumo de azeite de oliva sobre parâmetros cardiometabólicos clássicos e novos em indivíduos com diferentes níveis de risco cardiovascular. Métodos: O estudo foi do tipo clínico prospectivo, aleatorizado, placebo controlado, duplo cego baseado em intervenção nutricional. Indivíduos de ambos os sexos, distribuídos em grupos azeite de oliva (AO) e placebo (PL) receberam durante 8 semanas 3 g/d de azeite de oliva ou placebo. Todos os indivíduos foram classificados quanto ao risco cardiovascular, seguindo os critérios estabelecidos pelo Escore de Risco de Framingham (ERF). Nos momentos basal, T=4S e T=8S foram determinados o perfil clínico, antecedentes familiares de doenças, pressão arterial, consumo alimentar e nível de atividade física. A partir do plasma ou soro, obtidos após 12 h de jejum, foram determinados o perfil lipídico, as apolipoproteínas, o tamanho da HDL e da LDL, o conteúdo de LDL(-) e de NEFAS e atividade da paraoxonase. A aderência à intervenção foi monitorada por meios diretos (marcadores bioquímicos) e indiretos (registro de intercorrências). Resultados: O azeite de oliva foi efetivo em reduzir concentração de triacilglicerois dos indivíduos em alto risco cardiovascular (p=0,023 no T=4S e p=0,049 no T=8S) e a de LDL-C dos indivíduos com risco cardiovascular intermediário (p=0,045 no T=8S) no atual estudo. Observou-se também redução significativa na LDL(-), quando a amostra foi estratificada pelo ERF. Demais parâmetros permaneceram inalterados em função do tempo da intervenção e do ERF. Conclusão: Baixa suplementação (3 g/d) de azeite de oliva promoveu redução dos triacilglicerois, LDL-C e da LDL(-). Portanto, recomenda-se a incorporação de azeite de oliva na dieta brasileira ainda que em baixas doses. Sugere-se também que estudos adicionais usando doses maiores sejam realizados no sentido de identificar potenciais benefícios cardioprotetores adicionais associados ao consumo de azeite de oliva.

Introduction: Cardiovascular disease is the leading cause of premature morbidity and mortality worldwide, and accounts for a large part of the costs of resources devoted to public policy programs. In this context, the diet is an important tool in managing and reducing the risk of cardiovascular disease. Given that numerous studies show that consumption of omega 9 or food source changes positively several…

Advisors/Committee Members: Damasceno, Nágila Raquel Teixeira.

Subjects/Keywords: Cardiovascular Disease; Doença Cardiovascular; HDL; HDL; Intervenção; Intervention; LDL; LDL; Omega 9; Ômega 9

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Marangoni, A. B. (2013). Baixa suplementação de azeite de oliva reduz triaciglicerois e características lipídicas e oxidativas associadas à lipoproteína de baixa densidade em indivíduos com risco cardiovascular intermediário e alto. (Masters Thesis). University of São Paulo. Retrieved from http://www.teses.usp.br/teses/disponiveis/6/6138/tde-21102014-100904/ ;

Chicago Manual of Style (16^th Edition):

Marangoni, Adriane Bueno. “Baixa suplementação de azeite de oliva reduz triaciglicerois e características lipídicas e oxidativas associadas à lipoproteína de baixa densidade em indivíduos com risco cardiovascular intermediário e alto.” 2013. Masters Thesis, University of São Paulo. Accessed January 22, 2021. http://www.teses.usp.br/teses/disponiveis/6/6138/tde-21102014-100904/ ;.

MLA Handbook (7^th Edition):

Marangoni, Adriane Bueno. “Baixa suplementação de azeite de oliva reduz triaciglicerois e características lipídicas e oxidativas associadas à lipoproteína de baixa densidade em indivíduos com risco cardiovascular intermediário e alto.” 2013. Web. 22 Jan 2021.

Vancouver:

Marangoni AB. Baixa suplementação de azeite de oliva reduz triaciglicerois e características lipídicas e oxidativas associadas à lipoproteína de baixa densidade em indivíduos com risco cardiovascular intermediário e alto. [Internet] [Masters thesis]. University of São Paulo; 2013. [cited 2021 Jan 22]. Available from: http://www.teses.usp.br/teses/disponiveis/6/6138/tde-21102014-100904/ ;.

Council of Science Editors:

Marangoni AB. Baixa suplementação de azeite de oliva reduz triaciglicerois e características lipídicas e oxidativas associadas à lipoproteína de baixa densidade em indivíduos com risco cardiovascular intermediário e alto. [Masters Thesis]. University of São Paulo; 2013. Available from: http://www.teses.usp.br/teses/disponiveis/6/6138/tde-21102014-100904/ ;

21. Tonini, Gabriela Cristina. Efeitos da imunização com lipoproteína de baixa densidade oxidada na aterosclerose experimental murina e no modelo de doença renal crônica.

Degree: PhD, Imunologia, 2010, University of São Paulo

URL: http://www.teses.usp.br/teses/disponiveis/42/42133/tde-05102010-101603/ ;

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Anticorpos (Ac) anti-oxLDL estão envolvidos no desenvolvimento da lesão aterosclerótica. Induzimos a produção desses Ac através da imunização com oxLDL. Avaliamos os perfis lipídico e… (more)

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Anticorpos (Ac) anti-oxLDL estão envolvidos no desenvolvimento da lesão aterosclerótica. Induzimos a produção desses Ac através da imunização com oxLDL. Avaliamos os perfis lipídico e de Ac anti-oxLDL e anti-pepD (pepD é um peptídeo derivado da apoB). Animais apoE-/- possuem maiores níveis de lípides sorológicos em comparação com animais C57Bl/6, exceção da HDL. A imunização oxLDL não alterou este perfil. A imunização aumentou os títulos dos Ac IgM, IgG e IgG1 anti-oxLDL. Animais C57Bl/6, mas não os apoE-/- tiveram aumento dos Ac IgM anti-pepD após a imunização. Acreditamos que o aumento de anticorpos IgG1 deve-se ao adjuvante usado. A aterosclerose pode ocorrer com maior freqüência, em indivíduos portadores de doenças renais crônicas. Para estudar essa interferência submetemos os animais a um modelo de isquemia e reperfusão renal (I/R). A I/R promoveu aumento da concentração de TG, podendo agravar a aterosclerose. O aumento de Ac IgG anti-oxLDL promovidos pela I/R sugere que o processo inflamatório desencadeado por este procedimento, aumenta a oxidação de LDL. Desta forma, concluímos que a I/R pode ser considerado um procedimento pró-aterosclerótico que não pode ser revertido pela imunização com oxLDL.

Oxidized LDL (oxLDL) antibodies (Ab) are involved in the development of the atherosclerotic lesion. We induced the formation of such Ab through immunization with oxLDL. We evaluate the lipids and antibody (anti-oxLDL and anti-pepD pep D is a peptide derived from apolipoprotein B) profile. The apoE-/- mice used have higher seric levels of most lipids than C57Bl/6 animals, the exception being HDL. The oxLDL immunization does not change this profile. The immunization increased anti-oxLDL Ab e IgM, IgG and IgG1 titers. Anti-pepD IgM Ab increased with immunization in C57Bl/6 animals only, and not in apoE-/-. We believe the increased IgG1 Ab due to the adjuvant used. Atherosclerosis has a higher incidence in individuals with chronic renal conditions. In order to study such interference, we submitted the animals to a renal ischemia and reperfusion model (I/R). The I/R procedure increased TG concentration, what can make atherosclerosis more severe. The increase in IgG anti-oxLDL antibody caused by I/R suggests that the inflammation process set by the procedure increases LDL oxidation. This way, we conclude that the I/R can be considered a pro-atherosclerotic procedure. The oxLDL immunization was not able to revert the atherogenic effect caused by I/R model.

Advisors/Committee Members: Gidlund, Magnus Ake.

Subjects/Keywords: Arterioscherosis; Arteriosclerose; Immunization; Imunização; LDL lipoproteins; Lipoproteínas LDL; Nefropatias; Nephropathies; Peptídeos; Peptides

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Tonini, G. C. (2010). Efeitos da imunização com lipoproteína de baixa densidade oxidada na aterosclerose experimental murina e no modelo de doença renal crônica. (Doctoral Dissertation). University of São Paulo. Retrieved from http://www.teses.usp.br/teses/disponiveis/42/42133/tde-05102010-101603/ ;

Chicago Manual of Style (16^th Edition):

Tonini, Gabriela Cristina. “Efeitos da imunização com lipoproteína de baixa densidade oxidada na aterosclerose experimental murina e no modelo de doença renal crônica.” 2010. Doctoral Dissertation, University of São Paulo. Accessed January 22, 2021. http://www.teses.usp.br/teses/disponiveis/42/42133/tde-05102010-101603/ ;.

MLA Handbook (7^th Edition):

Tonini, Gabriela Cristina. “Efeitos da imunização com lipoproteína de baixa densidade oxidada na aterosclerose experimental murina e no modelo de doença renal crônica.” 2010. Web. 22 Jan 2021.

Vancouver:

Tonini GC. Efeitos da imunização com lipoproteína de baixa densidade oxidada na aterosclerose experimental murina e no modelo de doença renal crônica. [Internet] [Doctoral dissertation]. University of São Paulo; 2010. [cited 2021 Jan 22]. Available from: http://www.teses.usp.br/teses/disponiveis/42/42133/tde-05102010-101603/ ;.

Council of Science Editors:

Tonini GC. Efeitos da imunização com lipoproteína de baixa densidade oxidada na aterosclerose experimental murina e no modelo de doença renal crônica. [Doctoral Dissertation]. University of São Paulo; 2010. Available from: http://www.teses.usp.br/teses/disponiveis/42/42133/tde-05102010-101603/ ;

22. Mendes, Sérgio. Captação pelo carcinoma de mama e pelo linfonodo axilar de uma nanoemulsão lipídica administrada por injeção no tecido mamário locorregional.

Degree: PhD, Distúrbios Genéticos de Desenvolvimento e Metabolismo, 2008, University of São Paulo

URL: http://www.teses.usp.br/teses/disponiveis/5/5166/tde-25062008-160623/ ;

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Em trabalhos anteriores, mostrou-se que uma nanoemulsão lipídica denomi-nada LDE após injeção endovenosa, em pacientes com carcinoma mamário e outros tumores sólidos concentra-se nos tecidos… (more)

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Em trabalhos anteriores, mostrou-se que uma nanoemulsão lipídica denomi-nada LDE após injeção endovenosa, em pacientes com carcinoma mamário e outros tumores sólidos concentra-se nos tecidos neoplásicos e pode direcionar especificamente agentes quimioterápicos ao tumor. Estudos clínicos mostraram que a LDE diminui acentuadamente os efeitos tóxicos desses agentes e em estudos com animais de experimentação não reduz seus efeitos antitumorais. No presente estudo, testamos a hipótese de a LDE injetada por via locorregional poderia concentrar-se no tumor de mama e nos linfonodos axilares da mama comprometida, visando futuras aplica-ções do sistema na quimioterapia neoadjuvante desse tumor. Três técnicas de injeção da LDE foram testadas em pacientes com carcinoma de mama avançado com tratamento cirúrgico pré-programado. A LDE marcada com colesterol radioativo foi injetada 12 horas antes da cirurgia, nas pacientes divididas em três grupos: Grupo 1 (G1, n=4): LDE injetada no parênquima mamário, a 5 cm da lesão ; Grupo 2 (G2, n=4): LDE injetada na região peri-tumoral; e Grupo 3 (G3 n=6): LDE injetada em região intratumoral. Este grupo foi subdividido em 2: em 2 pacientes realizaram cirurgia 2hs após a injeção da LDE e nas outras 4, 12hs após a injeção da LDE. Quantificou-se a capta-ção da LDE nos fragmentos de tecido tumoral e mamário normal e do linfonodo axilar retirados durante a cirurgia por contagem de radioatividade após extração lipídica dos tecidos. Os resultados evidenciaram que, em G1, houve maior captação da LDE em tecido normal, sugerindo se tratar de metodologia ina-dequada. Em G2, a captação da LDE foi quatro vezes maior no tecido tumo-ral do que no tecido normal, próximo, portanto, dos valores encontrados em estudos anteriores que utilizaram a injeção da LDE por via hematológica. Em G3, o valor médio de captação da LDE foi 53 vezes maior no tecido tumoral (>75%), com mínima captação pelo tecido mamário normal (<8%). A injeção intratumoral da LDE mostrou-se, portanto, metodologia ainda superior à inje-ção da LDE por via endovenosa para a utilização efetiva dessa nanoemulsão como terapia-alvo em tumores primários de mama.

In previous work had showed that a lipidic nanoemulsion called LDE after intravenous injection in patients with breast carcinoma and others solid tumors focuses in the neoplasic tissue and can target especially chemotherapist agents to the tumors. Clinical studies showed that LDE decreases the toxic effects of those agents and in studies with experimental animals don\'t reduce yours antitumoral effects. In the present study, we had test the hypothesis of the LDE injected by locoregional could focuses in the breast tumor and in the axillary lymph node of the compromised breast, targeting future applications of the system in the neoadjuvant chemotherapy of this tumor. Three techniques of injection of the LDE had test in patients with advanced breast carcinoma with sirurgic treatment pre-programmed. The LDE marked with radioactive cholesterol was injected 12 hours before the surgery, in…

Advisors/Committee Members: Maranhao, Raul Cavalcante.

Subjects/Keywords: Breast cancer; Chemotherapy; LDL lipoprotein; Li-poproteínas LDL; Lipidic nanoemulsion; Nanoparticulas lipídicas; Neoplasia mamária; Quimioterapia

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Mendes, S. (2008). Captação pelo carcinoma de mama e pelo linfonodo axilar de uma nanoemulsão lipídica administrada por injeção no tecido mamário locorregional. (Doctoral Dissertation). University of São Paulo. Retrieved from http://www.teses.usp.br/teses/disponiveis/5/5166/tde-25062008-160623/ ;

Chicago Manual of Style (16^th Edition):

Mendes, Sérgio. “Captação pelo carcinoma de mama e pelo linfonodo axilar de uma nanoemulsão lipídica administrada por injeção no tecido mamário locorregional.” 2008. Doctoral Dissertation, University of São Paulo. Accessed January 22, 2021. http://www.teses.usp.br/teses/disponiveis/5/5166/tde-25062008-160623/ ;.

MLA Handbook (7^th Edition):

Mendes, Sérgio. “Captação pelo carcinoma de mama e pelo linfonodo axilar de uma nanoemulsão lipídica administrada por injeção no tecido mamário locorregional.” 2008. Web. 22 Jan 2021.

Vancouver:

Mendes S. Captação pelo carcinoma de mama e pelo linfonodo axilar de uma nanoemulsão lipídica administrada por injeção no tecido mamário locorregional. [Internet] [Doctoral dissertation]. University of São Paulo; 2008. [cited 2021 Jan 22]. Available from: http://www.teses.usp.br/teses/disponiveis/5/5166/tde-25062008-160623/ ;.

Council of Science Editors:

Mendes S. Captação pelo carcinoma de mama e pelo linfonodo axilar de uma nanoemulsão lipídica administrada por injeção no tecido mamário locorregional. [Doctoral Dissertation]. University of São Paulo; 2008. Available from: http://www.teses.usp.br/teses/disponiveis/5/5166/tde-25062008-160623/ ;

23. Natal, Aline Martins Duboc. Influência do pH na interação do Photofrin®, Photogem® e Photosan® com DMPC e lipoproteína de baixa densidade.

Degree: Mestrado, Química Analítica, 2007, University of São Paulo

URL: http://www.teses.usp.br/teses/disponiveis/75/75132/tde-27032008-110609/ ;

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O efeito do fotossensibilizador na estrutura biológica não é apenas influenciado por suas propriedades fotofísicas, mas também por sua interação específica com biosistemas.Além disso, a… (more)

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O efeito do fotossensibilizador na estrutura biológica não é apenas influenciado por suas propriedades fotofísicas, mas também por sua interação específica com biosistemas.Além disso, a localização do fotossensibilizador no tecido tumoral é um importante fator que resulta em diferentes mecanismos de destruição do tumor. Muitos fotossensibilizadores, após administração sistêmica, se ligam às proteínas plasmáticas e com isso são distribuídos em diferentes sítios no organismo. Os fotossensibilizadores hidrofílicos são largamente transportados por albuminas e globilinas e se acumulam preferencialmente no estroma vascular dos tumores. Entretanto, fotossensibilizadores mais hidrofóbicos se ligam às lipoproteínas, principalmente LDL, que promove a entrada do FS na célula através de endocitose mediado por receptor. Sendo assim, a localização do FS depende de sua ligação com as deferentes proteínas plasmáticas, sua farmacocinética e também é influenciada pela diferença entre o tecido normal e tumoral. O tecido tumoral tem pH mais baixo e maior expressão de receptores de LDL do que os tecidos normais, aumentando a seletividade dos FSs as células tumorais. A incorporação de FS hidrofóbicos em lipossomas para a administração sistêmica pode realçar ao transporte deste pelas lipoproteínas. No presente trabalho estudou-se a influência do pH na interação de fotossensibilizadores com lipossomas de DMPC e LDL. Os fotossensibilizadores utilizados nesse estudo foram Photofrin®, Photogem® e Photosan® que são derivados de hematoporfirinas. A metodologia empregada constitui de variação das concentrações de DMPC e LDL para os seguintes valores de pHs 5,0; 7,4 e 9,0, esse último pH utilizou-se somente para DMPC. O complexo FS - DMPC foi obtido por incubação dos FSs na concentração de 10 micro g.mL-1 com diferentes concentrações de DMPC (0 a 400 micro M) por trinta minutos no escuro. Isolou-se o LDL do plasma humano por ultracentrifugação por gradiente de densidade. Após a separação, o complexo FS - LDL foi obtido por incubação (12 horas no escuro) do FS na concentração 10 micro g.mL-1 com diferentes concentrações de LDL (0 a 0,04 micro M). O comportamento desses complexos foi analisado por espectroscopia de absorção ótica e por espectroscopia de fluorescência.

The effect of a photosensitizing compound on biological structures is governed not only by its photophysical properties but also by the specificity of its interaction with biosystems. Moreover, localization of the photosensitizer in the tumor tissue is an important factor affecting the outcome as well as mechanism leading to tumor destruction. Following administration, most photosensitizers are bound to blood components and delivered to different sites in the organism. It is generally accepted that hydrophilic photosensitizers are largely transported by albumins and globulins and mainly accumulate in the vascular stroma of tumors. More hydrophobic sensitizers are bound to lipoproteins, which promote drug internalization by cells through endocytosis of the lipoprotein carrier. In…

Advisors/Committee Members: Imasato, Hidetake.

Subjects/Keywords: DMPC; DMPC; fotossensibilizador; LDL; LDL; lipoprotein; lipoproteina; Photofrin; Photofrin; Photogem; Photogem; Photosan; Photosan; photosensitizer

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Natal, A. M. D. (2007). Influência do pH na interação do Photofrin®, Photogem® e Photosan® com DMPC e lipoproteína de baixa densidade. (Masters Thesis). University of São Paulo. Retrieved from http://www.teses.usp.br/teses/disponiveis/75/75132/tde-27032008-110609/ ;

Chicago Manual of Style (16^th Edition):

Natal, Aline Martins Duboc. “Influência do pH na interação do Photofrin®, Photogem® e Photosan® com DMPC e lipoproteína de baixa densidade.” 2007. Masters Thesis, University of São Paulo. Accessed January 22, 2021. http://www.teses.usp.br/teses/disponiveis/75/75132/tde-27032008-110609/ ;.

MLA Handbook (7^th Edition):

Natal, Aline Martins Duboc. “Influência do pH na interação do Photofrin®, Photogem® e Photosan® com DMPC e lipoproteína de baixa densidade.” 2007. Web. 22 Jan 2021.

Vancouver:

Natal AMD. Influência do pH na interação do Photofrin®, Photogem® e Photosan® com DMPC e lipoproteína de baixa densidade. [Internet] [Masters thesis]. University of São Paulo; 2007. [cited 2021 Jan 22]. Available from: http://www.teses.usp.br/teses/disponiveis/75/75132/tde-27032008-110609/ ;.

Council of Science Editors:

Natal AMD. Influência do pH na interação do Photofrin®, Photogem® e Photosan® com DMPC e lipoproteína de baixa densidade. [Masters Thesis]. University of São Paulo; 2007. Available from: http://www.teses.usp.br/teses/disponiveis/75/75132/tde-27032008-110609/ ;

24. Triantafyllou, Konstantinos. Η οξειδωμένη LDL χοληστερόλη σαν δείκτης του αθηρωματικού φορτίου στα στεφανιαία και στα περιφερικά αγγεία.

Degree: 2013, National and Kapodistrian University of Athens; Εθνικό και Καποδιστριακό Πανεπιστήμιο Αθηνών (ΕΚΠΑ)

URL: http://hdl.handle.net/10442/hedi/40923

► Background: Oxidized low-density lipoprotein (oxLDL) has been shown to hold an important role in atherosclerosis initiation and progression. We investigated the association of circulating oxLDL… (more)

▼ Background: Oxidized low-density lipoprotein (oxLDL) has been shown to hold an important role in atherosclerosis initiation and progression. We investigated the association of circulating oxLDL to coronary and peripheral atherosclerosis severity among patients with recently diagnosed or suspected CAD.Methods: The study comprised 196 patients (91.8% men), all ≤ 65 year-old without comorbidities, who had their first coronary angiogram after an acute coronary syndrome (85.7%) or for recently diagnosed or suspected stable CAD (14.3%). The number of major coronary arteries with stenosis ≥50% (CS) and Hamsten coronary atherosclerosis score (HS) were used as indices of CAD severity and extent respectively. Arterial B-mode ultrasound was performed bilaterally to measure common carotid (CC) and common femoral (CF) maximal intima-media thickness (IMT) and maximal plaque thickness (PT). A full lipid panel was acquired and hs-CRP was measured. Circulating oxLDL was measured with a monoclonal antibody-based (4E6) enzyme-linked immunosorbent assay. Pearson’s and Spearman correlations, linear and logistic regression models and ROC curves were used for statistical analysis.Results: OxLDL had a significant positive correlation with total cholesterol (r=0.506, p <0.001), LDL (r=0.496, p <0.001), nonHDL (r=0.505, p <0.001) and apo B-100 (r=0.476, p <0.001) but not with other lipid parameters. Furthermore oxLDL correlated significantly and negatively with age (r=-0.213, p=0.004) and positively with body mass index (r=0.165, p=0.030). Considering the negative correlation of oxLDL with age and the cut-off point of 55 years that defines premature CAD in men further analysis focused on male patients. For the male population of the study (180 of 196 patients) a weak but significant relationship was found between oxLDL and CS (r=0.169, p=0.033) but not between oxLDL and HS (r=0.124, p=0.130). OxLDL did not correlate significantly with the carotid and femoral atherosclerosis indices used and no other lipid parameter or hs-CRP did correlate significantly with any of the coronary or peripheral atherosclerosis indices. OxLDL was significantly higher among men ≤ 55 year-old (n=102, mean age: 48±5 years) versus men > 55 year-old (n=78, mean age: 60±3 years): 99±46 U/L vs 84±32 U/L respectively, p=0.023. A) Among patients ≤55 year-old oxLDL significantly correlated with both CAD scores (CS: r=0.243, p=0.021 and HS: r=0.244, p=0.024). Those in the highest (≥107 U/L) compared to those in the lowest (<77 U/L) oxLDL concentration tertile, had higher CAD scores (CS: 1.9±0.9 vs 1.3±1, p=0.012 & HS: 2.6±1.7 vs 1.6±1.1, p=0.01) and more often multi-vessel CAD (in 70% vs 34.5% of cases respectively, p=0.009). They were also more likely to have multi-vessel CAD, after adjustment for risk factors, age, body mass index, statin treatment, CIMT and apoB-100 (adjusted OR: 3.146, 95% CI: 1.220-8.112, p=0.018). Furthermore, at ROC curve analysis oxLDL was found to be a significant albeit modest predictor of multi-vessel CAD (AUC=0.652, 95% CI: 0.539-0.765 p=0.013).…

Subjects/Keywords: Οξειδωμένη LDL; Αθηροσκλήρωση; Σταιφανιαία νόσος; Βιοδείκτης; Λιποπρωτεΐνες; Oxidized LDL; Atherosclerosis; Conorary artery disease; Biomarker; Lipoproteins

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Triantafyllou, K. (2013). Η οξειδωμένη LDL χοληστερόλη σαν δείκτης του αθηρωματικού φορτίου στα στεφανιαία και στα περιφερικά αγγεία. (Thesis). National and Kapodistrian University of Athens; Εθνικό και Καποδιστριακό Πανεπιστήμιο Αθηνών (ΕΚΠΑ). Retrieved from http://hdl.handle.net/10442/hedi/40923

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Triantafyllou, Konstantinos. “Η οξειδωμένη LDL χοληστερόλη σαν δείκτης του αθηρωματικού φορτίου στα στεφανιαία και στα περιφερικά αγγεία.” 2013. Thesis, National and Kapodistrian University of Athens; Εθνικό και Καποδιστριακό Πανεπιστήμιο Αθηνών (ΕΚΠΑ). Accessed January 22, 2021. http://hdl.handle.net/10442/hedi/40923.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Triantafyllou, Konstantinos. “Η οξειδωμένη LDL χοληστερόλη σαν δείκτης του αθηρωματικού φορτίου στα στεφανιαία και στα περιφερικά αγγεία.” 2013. Web. 22 Jan 2021.

Vancouver:

Triantafyllou K. Η οξειδωμένη LDL χοληστερόλη σαν δείκτης του αθηρωματικού φορτίου στα στεφανιαία και στα περιφερικά αγγεία. [Internet] [Thesis]. National and Kapodistrian University of Athens; Εθνικό και Καποδιστριακό Πανεπιστήμιο Αθηνών (ΕΚΠΑ); 2013. [cited 2021 Jan 22]. Available from: http://hdl.handle.net/10442/hedi/40923.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Triantafyllou K. Η οξειδωμένη LDL χοληστερόλη σαν δείκτης του αθηρωματικού φορτίου στα στεφανιαία και στα περιφερικά αγγεία. [Thesis]. National and Kapodistrian University of Athens; Εθνικό και Καποδιστριακό Πανεπιστήμιο Αθηνών (ΕΚΠΑ); 2013. Available from: http://hdl.handle.net/10442/hedi/40923

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Universidade Estadual de Campinas

25. Freitas, Wladimir Magalhães de, 1967-. O papel do HDL colesterol na estratificação de risco cardiovascular entre indivíduos octogenários no contexto da prevenção primária = The role f HDL cholesterol in assessing cardiovascular risk in individuals with more than eighty years old in the primary prevention setting: The role f HDL cholesterol in assessing cardiovascular risk in individuals with more than eighty years old in the primary prevention setting.

Degree: 2016, Universidade Estadual de Campinas

URL: http://repositorio.unicamp.br/jspui/handle/REPOSIP/312486

► Abstract: Background/Objectives: Plasma level of low-density lipoprotein cholesterol (LDL-C) has become a major marker and the central preventive target for cardiovascular disease in a large… (more)

▼ Abstract: Background/Objectives: Plasma level of low-density lipoprotein cholesterol (LDL-C) has become a major marker and the central preventive target for cardiovascular disease in a large spectrum of clinical settings. However, recent reports have raised questions about the consistency of this concept in individuals aged of 80 years or older. Hence, the aim of this study was to evaluate in a carefully selected population if the association between lipid parameters and coronary atherosclerotic burden remains significant. Design, Setting, Participants, Measurements: Individuals aged of 80 years or older (n=208) who spontaneously sought primary prevention care and have never manifested cardiovascular disease, malnutrition, neoplastic or consumptive disease were enrolled for a cross-sectional analysis. Medical evaluation, anthropometric measurements, blood tests and cardiac computed tomography were obtained. Results: 1- In analyses adjusted for age, gender, diabetes, systolic and diastolic blood pressure, smoking and statin therapy no association was found between coronary calcium score (CCS) and LDL-C (1.79 (0.75-4.29)). There was no association between TG and CCS. The association between HDL-C and CCS was significant and robust in unadjusted (0.32 (0.15-0.67)) as well as in the fully adjusted analysis (0.34 (0.15-0.75)). Results: 2-All the coronary segments (>3.000) were assessed to the presence of coronary atherosclerotic plaques. Coronary atherosclerotic plaques by CCTA were classified according to previous studies: positive remodeling (PR), not calcified plaques (NCP) and spotty calcification (SC). Individuals were categorized in the presence or absence of PR, NCP and SC and highest and lowest to the last quartile of the lipid profile (HDL-C (? 63 vs < 63mg/Dl), LDL-C ? 138 vs < 138 mg/dL, TG (?156 vs <156 mg/dL)). Results-In adjusted analysis by age, gender, body fat, hypertension, creatinine, CRP, diabetes mellitus, smoking, physical activity and use of statin all type of plaques with instability phenotype were associated with age. No association was found between LDL_C or TG and atherosclerotic plaques. Association remains between remodeling plaques and HDL-C< 63 mg/dL in fully adjusted model (2.5(1.1,-6.3);0.040) Conclusion: The present study confirms in a healthy cohort of individuals aged of 80 years or more that while the association between LDL cholesterol and coronary atherosclerosis weakens with aging, the opposite occurs with the levels of HDL cholesterol Advisors/Committee Members: UNIVERSIDADE ESTADUAL DE CAMPINAS (CRUESP), Sposito, Andrei Carvalho, 1967- (advisor), Universidade Estadual de Campinas. Faculdade de Ciências Médicas (institution), Programa de Pós-Graduação em Ciências Médicas (nameofprogram), Giraldez, Viviane Zorzanelli Rocha (committee member), Cazita, Patricia Miralda (committee member), Tambascia, Marcos Antonio (committee member), Silva, Thiago Quinaglia Araujo Costa e (committee member).

Subjects/Keywords: Aterosclerose; HDL-colesterol; LDL-colesterol; Idosos; Atherosclerosis; Cholesterol, HDL; Cholesterol, LDL; Aged

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Freitas, Wladimir Magalhães de, 1. (2016). O papel do HDL colesterol na estratificação de risco cardiovascular entre indivíduos octogenários no contexto da prevenção primária = The role f HDL cholesterol in assessing cardiovascular risk in individuals with more than eighty years old in the primary prevention setting: The role f HDL cholesterol in assessing cardiovascular risk in individuals with more than eighty years old in the primary prevention setting. (Thesis). Universidade Estadual de Campinas. Retrieved from http://repositorio.unicamp.br/jspui/handle/REPOSIP/312486

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Freitas, Wladimir Magalhães de, 1967-. “O papel do HDL colesterol na estratificação de risco cardiovascular entre indivíduos octogenários no contexto da prevenção primária = The role f HDL cholesterol in assessing cardiovascular risk in individuals with more than eighty years old in the primary prevention setting: The role f HDL cholesterol in assessing cardiovascular risk in individuals with more than eighty years old in the primary prevention setting.” 2016. Thesis, Universidade Estadual de Campinas. Accessed January 22, 2021. http://repositorio.unicamp.br/jspui/handle/REPOSIP/312486.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Freitas, Wladimir Magalhães de, 1967-. “O papel do HDL colesterol na estratificação de risco cardiovascular entre indivíduos octogenários no contexto da prevenção primária = The role f HDL cholesterol in assessing cardiovascular risk in individuals with more than eighty years old in the primary prevention setting: The role f HDL cholesterol in assessing cardiovascular risk in individuals with more than eighty years old in the primary prevention setting.” 2016. Web. 22 Jan 2021.

Vancouver:

Freitas, Wladimir Magalhães de 1. O papel do HDL colesterol na estratificação de risco cardiovascular entre indivíduos octogenários no contexto da prevenção primária = The role f HDL cholesterol in assessing cardiovascular risk in individuals with more than eighty years old in the primary prevention setting: The role f HDL cholesterol in assessing cardiovascular risk in individuals with more than eighty years old in the primary prevention setting. [Internet] [Thesis]. Universidade Estadual de Campinas; 2016. [cited 2021 Jan 22]. Available from: http://repositorio.unicamp.br/jspui/handle/REPOSIP/312486.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Freitas, Wladimir Magalhães de 1. O papel do HDL colesterol na estratificação de risco cardiovascular entre indivíduos octogenários no contexto da prevenção primária = The role f HDL cholesterol in assessing cardiovascular risk in individuals with more than eighty years old in the primary prevention setting: The role f HDL cholesterol in assessing cardiovascular risk in individuals with more than eighty years old in the primary prevention setting. [Thesis]. Universidade Estadual de Campinas; 2016. Available from: http://repositorio.unicamp.br/jspui/handle/REPOSIP/312486

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Universidade Federal de Santa Maria

26. Rômulo Pillon Barcelos. AVALIAÇÃO DA ATIVIDADE DA ISATINA-3-N4-BENZILTIOSSEMICARBAZONA (IBTC) COMO ANTIOXIDANTE NA PROTEÇÃO DA LDL CONTRA A OXIDAÇÃO.

Degree: 2012, Universidade Federal de Santa Maria

URL: http://coralx.ufsm.br/tede/tde_busca/arquivo.php?codArquivo=4977

►

Muitos estudos têm revelado que a oxidação da lipoproteína de baixa densidade (LDL) é um fator pró-aterogênico e que o estresse oxidativo tem um papel… (more)

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Muitos estudos têm revelado que a oxidação da lipoproteína de baixa densidade (LDL) é um fator pró-aterogênico e que o estresse oxidativo tem um papel central na formação de LDL oxidada. As Tiossemicarbazonas são compostos usados em terapias anti-câncer, antiviral e antifúngica em humanos. No entanto, estudos sobre a atividade redox desta classe de moléculas ainda são bastante controversos. O objetivo deste trabalho foi avaliar a atividade antioxidante de um composto derivado de uma tiossemicarbazona: a isatina-3-N4-benziltiossemicarbazona (IBTC) e seu possível efeito protetor contra a oxidação da LDL in vitro. Tais atividades foram avaliadas através da formação de dienos conjugados em LDL e em soro humano induzidos por 2,2-azobis(2-amidinopropano diidroclorido) AAPH e por CuSO4; da perda da fluorescência do triptofano (Trp) em LDL induzida pelo CuSO4; da formação de substâncias reativas ao ácido tiobarbitúrico (TBARS) em LDL, plasma humano e em diferentes tecidos de ratos e do potencial antioxidante total (TRAP) do IBTC. A toxicidade do IBTC foi investigada em fatias da artéria aorta. Os resultados mostraram que a IBTC foi eficaz em reduzir a formação de dienos conjugados induzidos tanto pelo AAPH quanto pelo CuSO4, aumentando de modo concentração-dependente a fase lag. A IBTC também preveniu a perda acentuada da fluorescência do Trp, reduziu a formação de TBARS na LDL no plasma e nos tecidos isolados de ratos e não causou toxicidade em fatias da artéria aorta. Esses dados indicam que a IBTC pode ser considerado um promissor agente antioxidante e exibir propriedades anti-aterogênicas in vitro.

Several lines of evidence support the hypotheses that the oxidation of low density lipoprotein (LDL) is a proatherogenic factor. Oxidative stress is one of the causes of the overproduction of reactive species that increase the formation of oxidized LDL. Thiosemicarbazones are compounds used in anticancer, antiviral and antifungal therapy; however, their redox activities have been controversial. Thus, we tested, in vitro, the possible antioxidant activity of a thiosemicarbazone derivate, the isatin-3-N4-benzilthiosemicarbazone (IBTC). We measured the conjugated diene formation in serum and LDL induced by two oxidant agents, 2,2-azobis(2-amidinopropane dihydrochloride) (AAPH) and Cu2+, as well as the loss of tryptophan fluorescence in LDL induced by Cu2+. The total reactive antioxidant potential (TRAP), Thiobarbituric acid reactive substances (TBARS) formation in human LDL and plasma and in different rat tissues was also assessed. The toxicity of IBTC was measured using aortic slices viability assay. The results show that IBTC reduced the formation of conjugated dienes AAPH and Cu2+- induced increased in a dose-dependent manner the lag phase. IBTC also prevented the tryptophan fluorescence loss, and reduced the TBARS formation in LDL, plasma and rat tissues, showing no toxicity to aortic slices. These data indicate that IBTC is a good antioxidant and a promising antiatherogenic agent for further studies in vivo.

Advisors/Committee Members: Nilda Berenice de Vargas Barbosa, Roselei Fachinetto, Ricardo Brandão.

Subjects/Keywords: antioxidantes; aterosclerose; tiossemicarbazonas; CIENCIAS BIOLOGICAS; estresse oxidativo; LDL; thiosemicarbazones; oxidative stress; LDL; antioxidants; atherosclerosis

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Barcelos, R. P. (2012). AVALIAÇÃO DA ATIVIDADE DA ISATINA-3-N4-BENZILTIOSSEMICARBAZONA (IBTC) COMO ANTIOXIDANTE NA PROTEÇÃO DA LDL CONTRA A OXIDAÇÃO. (Thesis). Universidade Federal de Santa Maria. Retrieved from http://coralx.ufsm.br/tede/tde_busca/arquivo.php?codArquivo=4977

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Barcelos, Rômulo Pillon. “AVALIAÇÃO DA ATIVIDADE DA ISATINA-3-N4-BENZILTIOSSEMICARBAZONA (IBTC) COMO ANTIOXIDANTE NA PROTEÇÃO DA LDL CONTRA A OXIDAÇÃO.” 2012. Thesis, Universidade Federal de Santa Maria. Accessed January 22, 2021. http://coralx.ufsm.br/tede/tde_busca/arquivo.php?codArquivo=4977.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Barcelos, Rômulo Pillon. “AVALIAÇÃO DA ATIVIDADE DA ISATINA-3-N4-BENZILTIOSSEMICARBAZONA (IBTC) COMO ANTIOXIDANTE NA PROTEÇÃO DA LDL CONTRA A OXIDAÇÃO.” 2012. Web. 22 Jan 2021.

Vancouver:

Barcelos RP. AVALIAÇÃO DA ATIVIDADE DA ISATINA-3-N4-BENZILTIOSSEMICARBAZONA (IBTC) COMO ANTIOXIDANTE NA PROTEÇÃO DA LDL CONTRA A OXIDAÇÃO. [Internet] [Thesis]. Universidade Federal de Santa Maria; 2012. [cited 2021 Jan 22]. Available from: http://coralx.ufsm.br/tede/tde_busca/arquivo.php?codArquivo=4977.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Barcelos RP. AVALIAÇÃO DA ATIVIDADE DA ISATINA-3-N4-BENZILTIOSSEMICARBAZONA (IBTC) COMO ANTIOXIDANTE NA PROTEÇÃO DA LDL CONTRA A OXIDAÇÃO. [Thesis]. Universidade Federal de Santa Maria; 2012. Available from: http://coralx.ufsm.br/tede/tde_busca/arquivo.php?codArquivo=4977

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Universidade do Estado do Rio de Janeiro

27. Juliana Almeida Pandini. Avaliação da influência das absorbâncias obtidas em três comprimentos de onda da lipoproteína de baixa densidade sobre sua oxidação in vitro em pacientes com Diabetes Mellitus tipo 1 .

Degree: Master, 2009, Universidade do Estado do Rio de Janeiro

URL: http://www.bdtd.uerj.br/tde_busca/arquivo.php?codArquivo=1519 ;

►

Com a intensificação do controle glicêmico e maior sobrevida dos pacientes com diabetes mellitus tipo 1 (DM1), houve mudança progressiva das causas de mortalidade, com… (more)

▼

Com a intensificação do controle glicêmico e maior sobrevida dos pacientes com diabetes mellitus tipo 1 (DM1), houve mudança progressiva das causas de mortalidade, com maior destaque para a doença cardiovascular (DCV). Em função da LDL oxidada ser considerada ponto fundamental na patogênese da doença aterosclerótica, o presente estudo objetivou avaliar as absorbâncias obtidas nos diferentes comprimentos de onda dos fosfolipídios oxidados e o coeficiente de oxidação da partícula de LDL in vitro em pacientes com diabetes mellitus tipo 1. Foram avaliados 54 pacientes com DM1 com idade de 29,1 12,11 e de duração de doença de 14,6 8,43 e 28 voluntários não diabéticos. Os pacientes e voluntários foram submetidos à avaliação clínica e laboratorial onde foram mensurados perfil lipídico, glicêmico, ácido úrico, níveis pressóricos, excreção urinária de albumina e análise espectofotométrica da oxidação do LDL colesterol antes e 1, 3, 6 e 24 horas após adição de substância oxidante- sulfato de cobre. Os coeficientes de oxidação (Cox) do LDL dos dois grupos apresentaram leitura semelhante em todos os momentos com diferença significativa entre o tempo basal e após 1h (p<0, 001) e entre o momento após 3 e 6 horas após adição de sulfato de cobre ( p< 0,001) para os grupos avaliados. No modelo de regressão múltipla, o resultado obtido foi o modelo Cox = 8,246 13,93a -2,71b + 10,21c (R2=0,835), onde a, b e c representam as absorbâncias (205 nm, 232 nm e 280 nm, respectivamente). Concluímos que em nossa amostra os comprimentos de onda 232 nm e 280 nm referentes à leitura dos dienos conjugados e a fração protéica da LDL, respectivamente apresentaram maior importância na determinação do coeficiente de oxidação da partícula.

With the intensive glycemic control and longer survival of type 1 diabetes mellitus (T1DM) patients, cardiovascular disease has been the main cause of mortality. Oxidized LDL is considered a critical factor in the atherosclerotic process and the aim of our study was evaluate the absorbance of different wavelengths of oxidized phospholipids and oxidation rate of LDL particles in vitro in T1DM. We studied 54 patients aged 29.1 12.11 years and with a disease duration of 14.6 8.43 years and 28 non-diabetic volunteers. The two groups had carried where they were measured lipid profile, glucose, uric acid, blood pressure, urinary albumin excretion and spectrophotometry analysis of the oxidation of LDL cholesterol before and 1, 3, 6 and 24 hours after addition of substance oxidant copper sulfate. The coefficients of oxidation (Cox) of LDL from both groups had similar reading at all times with a significant difference between the baseline and after 1 hour (p <0 .001) and between the time after 3 and 6 hours after addition of sulfate copper (p <0 .001) for both groups. In the multiple regression model, the result was the Cox model = 8.246 to 13.93 to 10.21 + -2.71 b c (R2 = 0.835), here "a", "b" and "c" represent the absorbance (205 nm , 232 nm and 280 nm, respectively). We conclude that in our sample the wavelengths…

Advisors/Committee Members: Adriana Ribeiro Silva, Carla Maria Avesani, Cecilia Lacroix de Oliveira, Marilia de Brito Gomes.

Subjects/Keywords: Type 1 diabetes; Suscetibilidade; NUTRICAO; LDL oxidado; Diabetes tipo 1; Oxidized LDL; Susceptibility

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Pandini, J. A. (2009). Avaliação da influência das absorbâncias obtidas em três comprimentos de onda da lipoproteína de baixa densidade sobre sua oxidação in vitro em pacientes com Diabetes Mellitus tipo 1 . (Masters Thesis). Universidade do Estado do Rio de Janeiro. Retrieved from http://www.bdtd.uerj.br/tde_busca/arquivo.php?codArquivo=1519 ;

Chicago Manual of Style (16^th Edition):

Pandini, Juliana Almeida. “Avaliação da influência das absorbâncias obtidas em três comprimentos de onda da lipoproteína de baixa densidade sobre sua oxidação in vitro em pacientes com Diabetes Mellitus tipo 1 .” 2009. Masters Thesis, Universidade do Estado do Rio de Janeiro. Accessed January 22, 2021. http://www.bdtd.uerj.br/tde_busca/arquivo.php?codArquivo=1519 ;.

MLA Handbook (7^th Edition):

Pandini, Juliana Almeida. “Avaliação da influência das absorbâncias obtidas em três comprimentos de onda da lipoproteína de baixa densidade sobre sua oxidação in vitro em pacientes com Diabetes Mellitus tipo 1 .” 2009. Web. 22 Jan 2021.

Vancouver:

Pandini JA. Avaliação da influência das absorbâncias obtidas em três comprimentos de onda da lipoproteína de baixa densidade sobre sua oxidação in vitro em pacientes com Diabetes Mellitus tipo 1 . [Internet] [Masters thesis]. Universidade do Estado do Rio de Janeiro; 2009. [cited 2021 Jan 22]. Available from: http://www.bdtd.uerj.br/tde_busca/arquivo.php?codArquivo=1519 ;.

Council of Science Editors:

Pandini JA. Avaliação da influência das absorbâncias obtidas em três comprimentos de onda da lipoproteína de baixa densidade sobre sua oxidação in vitro em pacientes com Diabetes Mellitus tipo 1 . [Masters Thesis]. Universidade do Estado do Rio de Janeiro; 2009. Available from: http://www.bdtd.uerj.br/tde_busca/arquivo.php?codArquivo=1519 ;

Penn State University

28. Wang, Li. The Effects Of Monounsaturated Fatty Acid-enriched Diets With And Without Avocados On Cardio-metabolic Risk Factors.

Degree: 2015, Penn State University

URL: https://submit-etda.libraries.psu.edu/catalog/25111

► Dietary guidance recommends a healthy dietary pattern that is low in saturated fat (SFA) to lower low-density lipoprotein cholesterol (LDL-C), a primary target for cardiovascular… (more)

▼ Dietary guidance recommends a healthy dietary pattern that is low in saturated fat (SFA) to lower low-density lipoprotein cholesterol (LDL-C), a primary target for cardiovascular disease (CVD) risk reduction. Using either carbohydrates (CHO) or unsaturated fat to replace saturated fat in an average American diet may achieve a similar reduction in LDL-C, but a high intake of dietary CHO may increase triglycerides (TG), lower high density lipoprotein cholesterol (HDL-C), and increase small, dense LDL, along with promoting insulin resistance thereby increasing the risk of metabolic syndrome and type 2 diabetes. These cardio-metabolic risk factors also are targets for dietary intervention to achieve a greater CVD risk reduction. When SFA is replaced with unsaturated fat, the predominant fatty acid class is monounsaturated fatty acids (MUFA) because the recommended intake of polyunsaturated fatty acids (PUFA) is less than 10% of energy. Overall, the substitution of MUFA instead of CHO (especially refine grains and added sugar) for SFA calories may favorably affect other lipid risk factors of CVD. The oxidative modification of LDL particles is a key factor in the initial process of atherosclerosis. Dietary antioxidant vitamins, polyphenols, and other bioactive compounds from foods (e.g., fruits and vegetables) have been a focus of nutrition because of their role in improving antioxidant status and lowering LDL oxidation. Identifying foods that can beneficially improve multiple cardio-metabolic risk factors is needed for the primary prevention of CVD. Avocados are a nutrient-dense source of MUFA, rich in vitamins, minerals, fiber, phytosterols and polyphenols. It is unclear whether avocados affect cardio-metabolic risk factors beyond their fatty acid profile. This dissertation investigated the effects of a MUFA enriched moderate fat diets including one avocado per day on established and novel cardio-metabolic risk factors. A randomized, cross-over, controlled feeding trial was conducted with 45 healthy overweight/obese participants with baseline LDL-C levels in the 25-90th percentile. After a 2 week run-in average American diet (34% fat, 13% SFA, 11% MUFA, 51% carbohydrate, 16% protein) at baseline, three cholesterol-lowering diets (6-7% SFA) were fed (5 weeks each) with a random sequence: a lower-fat diet (LF: 24% fat, 7% SFA, 11% MUFA, 9% PUFA, 59% carbohydrate, 16-17% protein); and two moderate fat diets matched for macronutrients and fatty acids (34% fat, 6% SFA, 17% MUFA, 9% PUFA, 49% carbohydrate, 16-17% protein): the avocado diet included one fresh Hass avocado (136 g) per day, and the moderate fat diet provided mostly the same foods (as the avocado diet) but used high oleic acid oils and low fat dairy products to match the fatty acid profile of one avocado. All three cholesterol-lowering diets met current dietary recommendations on macronutrient percentage range and the serving amount for each food group. Specifically, the grain product in the lower fat diet contained more than half whole grains,… Advisors/Committee Members: Penny Margaret Kris Etherton, Dissertation Advisor/Co-Advisor, Penny Margaret Kris Etherton, Committee Chair/Co-Chair, Joshua D Lambert, Committee Member, Jeffrey Maurice Peters, Committee Member, Michael Thomas Green, Committee Member, Mosuk Chow, Committee Member.

Subjects/Keywords: avocado; antioxidant; CVD; LDL-C; lipid; lipoprotein; metabolic syndrome; MUFA; nutrition; oxidation; small dense LDL.

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Wang, L. (2015). The Effects Of Monounsaturated Fatty Acid-enriched Diets With And Without Avocados On Cardio-metabolic Risk Factors. (Thesis). Penn State University. Retrieved from https://submit-etda.libraries.psu.edu/catalog/25111

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Wang, Li. “The Effects Of Monounsaturated Fatty Acid-enriched Diets With And Without Avocados On Cardio-metabolic Risk Factors.” 2015. Thesis, Penn State University. Accessed January 22, 2021. https://submit-etda.libraries.psu.edu/catalog/25111.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Wang, Li. “The Effects Of Monounsaturated Fatty Acid-enriched Diets With And Without Avocados On Cardio-metabolic Risk Factors.” 2015. Web. 22 Jan 2021.

Vancouver:

Wang L. The Effects Of Monounsaturated Fatty Acid-enriched Diets With And Without Avocados On Cardio-metabolic Risk Factors. [Internet] [Thesis]. Penn State University; 2015. [cited 2021 Jan 22]. Available from: https://submit-etda.libraries.psu.edu/catalog/25111.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Wang L. The Effects Of Monounsaturated Fatty Acid-enriched Diets With And Without Avocados On Cardio-metabolic Risk Factors. [Thesis]. Penn State University; 2015. Available from: https://submit-etda.libraries.psu.edu/catalog/25111

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

29. Ribeiro, Rosa Adelaide Tavares. Hipercolesterolemia em jovens adolescentes.

Degree: 2011, Universidade Fernando Pessoa

URL: http://www.rcaap.pt/detail.jsp?id=oai:bdigital.ufp.pt:10284/2451

►

Trabalho apresentado à Universidade Fernando Pessoa como parte dos requisitos para obtenção do grau de Mestre em Ciências Farmacêuticas.

O colesterol encontra-se em todas as… (more)

▼

Trabalho apresentado à Universidade Fernando Pessoa como parte dos requisitos para obtenção do grau de Mestre em Ciências Farmacêuticas.

O colesterol encontra-se em todas as células do organismo humano. Se, por um lado, é vital, por exemplo, para a construção de novas células, por outro, pode acumular-se no nosso organismo, levando à deposição de gordura na parede das artérias. Encontra-se bem fundamentada a relação entre o aumento drástico de mortes por doença cardiovascular, nos países desenvolvidos, e o elevado nível de colesterol que se verifica em parte substancial da sua população. Sendo uma substância lipídica, necessita de transportadores específicos para circular no plasma, as lipoproteínas. Estas partículas designam-se de acordo com a sua densidade. As duas mais importantes são as lipoproteínas de alta densidade (HDL) e as lipoproteínas de baixa densidade (LDL). As primeiras têm como principal função remover o excesso de colesterol para o fígado. Pelo contrário, as segundas transportam o colesterol do fígado para as células. Este trabalho visa estudar a problemática da hipercolesterolemia, na população infantil e adolescente. Neste segmento populacional, esta condição assume especial importância, sobretudo quando se verifica a conjugação de altos níveis de LDL colesterol com baixos níveis de HDL colesterol, pelas consequências na saúde a longo prazo. O aumento muito significativo da hipercolesterolemia relaciona-se intimamente com o estilo de vida actual, que contempla diversos factores de risco, a serem analisados com detalhe ao longo do trabalho. Pela análise de uma grande variedade de dados estatísticos, procurou caracterizar-se cada um destes factores, no sentido de avaliar a sua efectiva contribuição para a prevalência da hipercolesterolemia, em crianças e jovens, e verificar a existência de possíveis correlações entre eles, que pudessem contribuir de forma decisiva para o aumento do risco. Cholesterol is found in all cells of the human organism. If, on the one hand, it is vital, for example, to build new cells, on the other hand, it can also accumulate in the body, leading to fat deposition in artery walls. There is a well-substantiated relationship between the dramatic increase in deaths caused by cardiovascular disease in developed countries and the high level of cholesterol occurring in a substantial share of its population. As a lipid substance, it requires specific transporters making it possible to circulate in plasma, designated lipoproteins. These particles are classified according to their density. The two most important are the high-density lipoproteins (HDL) and the low-density lipoproteins (LDL). The former are mainly responsible for removing the excess of cholesterol to the liver. On the contrary, the latter carry cholesterol from the liver to the cells. This work aims to study the problem of hypercholesterolemia in children and adolescents. In this population segment, this condition is of extraordinary importance, especially when there is a…

Advisors/Committee Members: Castro, Ana Rita.

Subjects/Keywords: Hipercolesterolemia; LDL; HDL; Risco cardiovascular; Crianças; Adolescente; Hypercholesterolemia; LDL; HDL; Cardiovascular risk; Children; Adolescents

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Ribeiro, R. A. T. (2011). Hipercolesterolemia em jovens adolescentes. (Thesis). Universidade Fernando Pessoa. Retrieved from http://www.rcaap.pt/detail.jsp?id=oai:bdigital.ufp.pt:10284/2451

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Ribeiro, Rosa Adelaide Tavares. “Hipercolesterolemia em jovens adolescentes.” 2011. Thesis, Universidade Fernando Pessoa. Accessed January 22, 2021. http://www.rcaap.pt/detail.jsp?id=oai:bdigital.ufp.pt:10284/2451.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Ribeiro, Rosa Adelaide Tavares. “Hipercolesterolemia em jovens adolescentes.” 2011. Web. 22 Jan 2021.

Vancouver:

Ribeiro RAT. Hipercolesterolemia em jovens adolescentes. [Internet] [Thesis]. Universidade Fernando Pessoa; 2011. [cited 2021 Jan 22]. Available from: http://www.rcaap.pt/detail.jsp?id=oai:bdigital.ufp.pt:10284/2451.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Ribeiro RAT. Hipercolesterolemia em jovens adolescentes. [Thesis]. Universidade Fernando Pessoa; 2011. Available from: http://www.rcaap.pt/detail.jsp?id=oai:bdigital.ufp.pt:10284/2451

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Vienna

30. Konecsni, Tuende. Role of the β-propeller domain of low-density lipoprotein receptor in human rhinovirus infection.

Degree: 2010, University of Vienna

URL: http://othes.univie.ac.at/9192/

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Drei Vertreter der low-density Lipoprotein Rezeptor Familie, LDLR selbst, very-LDLR, und LDLR related protein (LRP) werden von minor group Rhinoviren des Menschen (HRVs) als Rezeptoren… (more)

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Drei Vertreter der low-density Lipoprotein Rezeptor Familie, LDLR selbst, very-LDLR, und LDLR related protein (LRP) werden von minor group Rhinoviren des Menschen (HRVs) als Rezeptoren verwendet. Während ICAM-1, der Rezeptor der major group der HRVs, aktiv am viralen uncoating beteiligt ist, wurden minor group Rezeptoren als passive Vehikel betrachtet, die das Virus in Endosomen transportieren, wo sie ein Milieu mit niedrigem pH vorfinden. Für die YWTD β-Propeller Domaine des LDL-Rezeptors wurde jedoch gezeigt, dass sie an der Abdissoziation gebundenen LDLs bei niedrigem pH beteiligt ist. Wir wollten daher herausfinden, ob sie auch aktiv an der Infektion mit HRVs teilnimmt. Es existiert keine humane Zell Linie, die LDLR in Abwesenheit von VLDLR oder LRP exprimiert. Daher wurden in unseren Untersuchungen CHO-ldla7 Zellen verwendet, die keinen endogenen LDL-Rezeptor exprimieren. Diese waren stabil transfiziert um entweder Wildtyp oder β-Propeller negative humane LDL-Rezeptoren zu exprimieren. Mit Hilfe dieser Zellen untersuchten wir den möglichen Einfluss des β-Propellers auf die Konversion von HRV2 in subvirale Partikel als eine Funktion des niedrigen pH und die Freisetzung des Virus vom Rezeptor. Wenn HRV2 an propeller-negativen LDLR gebunden war ein niedrigerer pH wurde für die Konversion benötigt. Dies deutet darauf hin, dass die hoch-avide Rezeptorbindung das Virus in seiner nativen Konformation stabilisiert. Infektionsversuche zeigten eine zeitliche Verzögerung in der intrazellulären viralen Konversion, im Abbau und in der viralen de novo Synthese in der Abwesenheit der β-Propeller-Domaine. Wir schließen daraus, dass die Funktion des β-Propellers bei niedrigem pH auch dann signifikant ist, wenn der Ligand ein Rhinovirus ist. Er erleichtert die virale Konversion im Inneren von Endosomen, indem er den Virus-stabilisierenden Effekt des mit hoher Avidität bindenden LDLR reduziert. Dadurch begünstigt er die Infektion.

Three members of the low-density lipoprotein receptor (LDLR) family, LDLR proper, very-LDLR (VLDLR) and LDLR-related protein (LRP) act as minor group human rhinovirus (HRV) receptors. Whereas ICAM-1, the receptor of the major group of HRVs, actively contributes to viral uncoating, minor group receptors were rather considered passive vehicles just delivering virus to the low pH environment of endosomes. However, the YWTDβ-propeller domain of LDLR has been shown to be involved in the dissociation of bound LDL at acidic pH. We thus aimed to clarify whether it also actively contributes to HRV infection. No human cell line expressing LDLR in the absence of VLDLR or LRP is available. Therefore, CHO-ldla7 cells which are deficient in endogenous LDLR and are stably transfected to express either wild-type or β-propeller negative human LDLR were used to investigate low pH-triggered conversion of HRV2 and its release from the receptor. Lower pH was required for conversion when HRV2 was attached to the propeller-negative LDLR, indicating that the high-avidity receptor-binding stabilizes the virus in its native…

Subjects/Keywords: 42.32 Virologie; LDL-Rezeptor / β-Propeller / Human Rhinovirus; LDL-receptor / β-propeller / human rhinovirus

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APA (6^th Edition):

Konecsni, T. (2010). Role of the β-propeller domain of low-density lipoprotein receptor in human rhinovirus infection. (Thesis). University of Vienna. Retrieved from http://othes.univie.ac.at/9192/

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Konecsni, Tuende. “Role of the β-propeller domain of low-density lipoprotein receptor in human rhinovirus infection.” 2010. Thesis, University of Vienna. Accessed January 22, 2021. http://othes.univie.ac.at/9192/.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Konecsni, Tuende. “Role of the β-propeller domain of low-density lipoprotein receptor in human rhinovirus infection.” 2010. Web. 22 Jan 2021.

Vancouver:

Konecsni T. Role of the β-propeller domain of low-density lipoprotein receptor in human rhinovirus infection. [Internet] [Thesis]. University of Vienna; 2010. [cited 2021 Jan 22]. Available from: http://othes.univie.ac.at/9192/.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Konecsni T. Role of the β-propeller domain of low-density lipoprotein receptor in human rhinovirus infection. [Thesis]. University of Vienna; 2010. Available from: http://othes.univie.ac.at/9192/

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

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