subject:(L Tyrosine)

1. Ferreira, Soraia Nunes. Desenvolvimento de novos suportes cromatográficos para purificação do DNA plasmídeo.

Degree: 2014, RCAAP

URL: https://www.rcaap.pt/detail.jsp?id=oai:ubibliorum.ubi.pt:10400.6/6098

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Nos últimos anos, a aplicação de DNA plasmídeo (pDNA) em terapia génica ou vacinas de DNA tem-se revelado uma ferramenta com potencial aplicação no tratamento… (more)

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Nos últimos anos, a aplicação de DNA plasmídeo (pDNA) em terapia génica ou vacinas de DNA tem-se revelado uma ferramenta com potencial aplicação no tratamento de inúmeras doenças. Uma vez que esta abordagem recorre a vetores não-virais, é mais segura em termos de ativação de oncogenes e apresenta uma diminuição de reações do sistema imunitário. Deste modo, a indústria farmacêutica procura obter pDNA em maiores quantidades e isento de contaminantes (RNA, DNA genómico, endotoxinas, proteínas). Uma técnica que permite purificar o pDNA é a cromatografia de afinidade (AC). Esta metodologia baseia-se em interações específicas entre ligandos e o pDNA, permitindo a eliminação de alguns passos de purificação. A utilização de aminoácidos como ligandos de afinidade revelou ser uma abordagem promissora para purificar ácidos nucleicos, pois baseia-se na bioespecificidade aminoácido-nucleótido. A L-histidina e a L-arginina permitiram isolar a isoforma superenrolada (sc) do pDNA, indicando a presença de interações específicas entre o pDNA e os aminoácidos. Neste trabalho são apresentados os resultados de três ligandos, a L-tirosina e os dipeptídeos L-tirosina-L-tirosina e L-tirosina-L-arginina. Os dipeptídeos foram sintetizados de acordo com o método clássico de síntese química, que envolve a proteção do grupo amina e do grupo carboxílico de cada aminoácido, e posterior formação da ligação peptídica e por fim desproteção usando TFA (ácido trifluoracético). De seguida, os ligandos foram imobilizados na Sepharose CL-6B através da ativação dos grupos epóxi com o reagente 1,4-butanediol diglicidil éter. O suporte foi caracterizado por HR-MAS (Ressonância Magnética de Alta Resolução de Ângulo Mágico) RMN. A técnica de STD-RMN (Diferença da Transferência de saturação por ressonância magnética nuclear) permitiu identificar os grupos preferenciais dos 5´-mononucleótidos que interagem com os suportes Sepharose-L-tirosina, Sepharose-Ltirosina- L-tirosina e Sepharose-L-tirosina-L-arginina. Utilizando a técnica de RPS (Ressonância Plasmónica de Superfície), foi analisada a afinidade de cada um dos ligandos em estudo em relação a cada uma das isoformas de pVAX1-LacZ a diferentes temperaturas (T=10°C e 25°C) e usando diferentes tampões (Tris-HCl 10 mM e Hepes ácido 10 mM). Desta forma, a utilização das técnicas STD-RMN e SPR permitiu fazer o screening das interações dos suportes-5’- mononucleótidos e determinar a afinidade do complexo ligando: pDNA, para futura aplicação em AC.

In the last years, the use of plasmid DNA (pDNA) in gene therapy or DNA vaccines has been proved to be a tool with potential application in the treatment of diseases. Since this approach uses non-viral vectors, it is safer in terms of oncogene activation and it presents a decrease of the immune system response. In this way, the pharmaceutical industry needs to obtain pDNA in larger quantities and free of contaminants (RNA, genomic DNA, endotoxins, proteins). A technique that allows purifying pDNA is affinity chromatography (AC). This method is based in specific…

Advisors/Committee Members: Cruz, Carla Patrícia Alves Freire Madeira, Sousa, Fani Pereira de.

Subjects/Keywords: Affinity Chromatography; L-Tyrosine; L-Tyrosine-L-Arginine; L-Tyrosine-L-Tyrosine; Plasmid Dna; Pvax1-Lacz; Spr; Std-Nmr; Domínio/Área Científica::Engenharia e Tecnologia:: Outras Engenharias e Tecnologias

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Ferreira, S. N. (2014). Desenvolvimento de novos suportes cromatográficos para purificação do DNA plasmídeo. (Thesis). RCAAP. Retrieved from https://www.rcaap.pt/detail.jsp?id=oai:ubibliorum.ubi.pt:10400.6/6098

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Ferreira, Soraia Nunes. “Desenvolvimento de novos suportes cromatográficos para purificação do DNA plasmídeo.” 2014. Thesis, RCAAP. Accessed January 18, 2021. https://www.rcaap.pt/detail.jsp?id=oai:ubibliorum.ubi.pt:10400.6/6098.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Ferreira, Soraia Nunes. “Desenvolvimento de novos suportes cromatográficos para purificação do DNA plasmídeo.” 2014. Web. 18 Jan 2021.

Vancouver:

Ferreira SN. Desenvolvimento de novos suportes cromatográficos para purificação do DNA plasmídeo. [Internet] [Thesis]. RCAAP; 2014. [cited 2021 Jan 18]. Available from: https://www.rcaap.pt/detail.jsp?id=oai:ubibliorum.ubi.pt:10400.6/6098.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Ferreira SN. Desenvolvimento de novos suportes cromatográficos para purificação do DNA plasmídeo. [Thesis]. RCAAP; 2014. Available from: https://www.rcaap.pt/detail.jsp?id=oai:ubibliorum.ubi.pt:10400.6/6098

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Anna University

2. Anandan P. Studies on the growth aspects and characterization of some amino acids based semiorganic nonlinear optical single crystals;.

Degree: 2013, Anna University

URL: http://shodhganga.inflibnet.ac.in/handle/10603/10391

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Single crystals provide the purest form of samples for basic research and also serve as the base components for large number of devices. It has… (more)

Subjects/Keywords: Amino acids; semiorganic nonlinear optical single crystal; L-arginine phosphate; L-histidine; L-tyrosine

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

P, A. (2013). Studies on the growth aspects and characterization of some amino acids based semiorganic nonlinear optical single crystals;. (Thesis). Anna University. Retrieved from http://shodhganga.inflibnet.ac.in/handle/10603/10391

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

P, Anandan. “Studies on the growth aspects and characterization of some amino acids based semiorganic nonlinear optical single crystals;.” 2013. Thesis, Anna University. Accessed January 18, 2021. http://shodhganga.inflibnet.ac.in/handle/10603/10391.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

P, Anandan. “Studies on the growth aspects and characterization of some amino acids based semiorganic nonlinear optical single crystals;.” 2013. Web. 18 Jan 2021.

Vancouver:

P A. Studies on the growth aspects and characterization of some amino acids based semiorganic nonlinear optical single crystals;. [Internet] [Thesis]. Anna University; 2013. [cited 2021 Jan 18]. Available from: http://shodhganga.inflibnet.ac.in/handle/10603/10391.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

P A. Studies on the growth aspects and characterization of some amino acids based semiorganic nonlinear optical single crystals;. [Thesis]. Anna University; 2013. Available from: http://shodhganga.inflibnet.ac.in/handle/10603/10391

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

3. Afonso, Adriana Alexandra Fernandes. Development and optimization of a microRNA purification technology by affinity chromatography, using the concept of QbD.

Degree: 2013, RCAAP

URL: https://www.rcaap.pt/detail.jsp?id=oai:ubibliorum.ubi.pt:10400.6/2772

► Atualmente, mais de 30 milhões de pessoas sofrem de demência em todo o mundo e, sem tratamento visível num futuro próximo, os números tendem a… (more)

Subjects/Keywords: Cromatografia de afinidade; Matrix O-phospho-L-tyrosine

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Afonso, A. A. F. (2013). Development and optimization of a microRNA purification technology by affinity chromatography, using the concept of QbD. (Thesis). RCAAP. Retrieved from https://www.rcaap.pt/detail.jsp?id=oai:ubibliorum.ubi.pt:10400.6/2772

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Afonso, Adriana Alexandra Fernandes. “Development and optimization of a microRNA purification technology by affinity chromatography, using the concept of QbD.” 2013. Thesis, RCAAP. Accessed January 18, 2021. https://www.rcaap.pt/detail.jsp?id=oai:ubibliorum.ubi.pt:10400.6/2772.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Afonso, Adriana Alexandra Fernandes. “Development and optimization of a microRNA purification technology by affinity chromatography, using the concept of QbD.” 2013. Web. 18 Jan 2021.

Vancouver:

Afonso AAF. Development and optimization of a microRNA purification technology by affinity chromatography, using the concept of QbD. [Internet] [Thesis]. RCAAP; 2013. [cited 2021 Jan 18]. Available from: https://www.rcaap.pt/detail.jsp?id=oai:ubibliorum.ubi.pt:10400.6/2772.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Afonso AAF. Development and optimization of a microRNA purification technology by affinity chromatography, using the concept of QbD. [Thesis]. RCAAP; 2013. Available from: https://www.rcaap.pt/detail.jsp?id=oai:ubibliorum.ubi.pt:10400.6/2772

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

4. Surwase, Shripad N. A novel approach for biotransformation of Ltyrosine to melanin by microbial system;.

Degree: 2014, Shivaji University

URL: http://shodhganga.inflibnet.ac.in/handle/10603/25463

newline

t16477

Advisors/Committee Members: Jadhav, J. P..

Subjects/Keywords: biotransformation; L-tyrosine; melanin; microbiology

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Surwase, S. N. (2014). A novel approach for biotransformation of Ltyrosine to melanin by microbial system;. (Thesis). Shivaji University. Retrieved from http://shodhganga.inflibnet.ac.in/handle/10603/25463

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Surwase, Shripad N. “A novel approach for biotransformation of Ltyrosine to melanin by microbial system;.” 2014. Thesis, Shivaji University. Accessed January 18, 2021. http://shodhganga.inflibnet.ac.in/handle/10603/25463.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Surwase, Shripad N. “A novel approach for biotransformation of Ltyrosine to melanin by microbial system;.” 2014. Web. 18 Jan 2021.

Vancouver:

Surwase SN. A novel approach for biotransformation of Ltyrosine to melanin by microbial system;. [Internet] [Thesis]. Shivaji University; 2014. [cited 2021 Jan 18]. Available from: http://shodhganga.inflibnet.ac.in/handle/10603/25463.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Surwase SN. A novel approach for biotransformation of Ltyrosine to melanin by microbial system;. [Thesis]. Shivaji University; 2014. Available from: http://shodhganga.inflibnet.ac.in/handle/10603/25463

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Georgia

5. Hartman, Nathan. The effect of nitrosative and oxidative stress on Helicobacter pylori.

Degree: 2016, University of Georgia

URL: http://hdl.handle.net/10724/35728

► The gastric pathogen Helicobacter pylori is able to withstand an onslaught of oxidative and nitrosative stress from the host immune system. One such stress arises… (more)

Subjects/Keywords: Helicobacter pylori; Peroxynitrite; Tyrosine nitration; Oxidative stress; Protein L-isoaspartate methyltransferase; Isoaspartate

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APA (6^th Edition):

Hartman, N. (2016). The effect of nitrosative and oxidative stress on Helicobacter pylori. (Thesis). University of Georgia. Retrieved from http://hdl.handle.net/10724/35728

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Hartman, Nathan. “The effect of nitrosative and oxidative stress on Helicobacter pylori.” 2016. Thesis, University of Georgia. Accessed January 18, 2021. http://hdl.handle.net/10724/35728.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Hartman, Nathan. “The effect of nitrosative and oxidative stress on Helicobacter pylori.” 2016. Web. 18 Jan 2021.

Vancouver:

Hartman N. The effect of nitrosative and oxidative stress on Helicobacter pylori. [Internet] [Thesis]. University of Georgia; 2016. [cited 2021 Jan 18]. Available from: http://hdl.handle.net/10724/35728.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Hartman N. The effect of nitrosative and oxidative stress on Helicobacter pylori. [Thesis]. University of Georgia; 2016. Available from: http://hdl.handle.net/10724/35728

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Akron

6. Shah, Kush. Receptor-Mediated Targeting to Enhance Therapeutic Efficacy of Chemotherapeutic Agents.

Degree: PhD, Integrated Bioscience, 2014, University of Akron

URL: http://rave.ohiolink.edu/etdc/view?acc_num=akron1390926627

► Dr. Paul Ehlrich introduced the concept of targeted drug delivery in 1904. With the advancement in technology, and molecular biology techniques, several new targeting… (more)

▼ Dr. Paul Ehlrich introduced the concept of targeted drug delivery in 1904. With the advancement in technology, and molecular biology techniques, several new targeting moieties have been developed in the past two decades. These targeting molecules improve the therapeutic efficacy of the drug, and alleviate systemic toxicity. Drug delivery devices, including, nanoparticles and polymer-drug pendant chains have been thoroughly investigated for targeting applications. Cancer has been the focus for development of efficient targeted drug delivery devices and new therapies because of poor prognosis, and socio-economic impact. One of the main aims of this research is to design and develop a targeted drug delivery device with the goal of achieving receptor mediated targeting to the tumor and alleviate systemic toxicity. Therefore, peptide targeted nanoparticles and hyaluronan based self-targeting polymer-chain pendant system have been developed and tested with two novel chemotherapeutic agents, resveratrol and MOMIPP. Peptides Lyp1 and p160 have been chosen for targeting L-tyrosine polyphosphate nanoparticles because of their ability to selectively bind with certain breast cancers. Lyp1 targeted nanoparticles show 5 and 7 fold greater attachment to MB157 and MB231 cells, while p160 targeted nanoparticles exhibit 3-fold greater attachment to MCF7 cells, under physiological flow compared to untargeted nanoparticles or non-cancerous cells. This attachment is stable since the attached nanoparticles do not detach at shear stresses encountered in the tumor interstitium. Hyaluronan, a hydrophilic biopolymer plays an important role in tumor development and metastasis, hence, several cancers over-express hyaluronan binding receptors, CD44 and RHAMM. The availability of multiple functional groups on hyaluronan allows for attachment of drugs, therefore, hyaluronan is an ideal candidate for development of self-targeted drug delivery device. Hyaluronan conjugates exhibit enhanced attachment to CD44 over-expressing MB157 cells compared to non-conjugated drug. Further, hyaluronan conjugated resveratrol and MOMIPP exhibit up to 12-fold greater toxicity against certain cancers in vitro. Hyaluronan also shows enhanced attachment to breast cancer cells under physiological flow conditions compared to non-cancerous cells. Animals injected with hyaluronan conjugates via tail-vein show greater accumulation in the tumors compared to kidneys and livers, which translates to a reduced tumor mass. Thus, these hyaluronan conjugates suggest hyaluronan's potential as a "magic bullet" to target and deliver therapeutic agents specifically to the cell of interest. Advisors/Committee Members: Yun, Yang (Advisor).

Subjects/Keywords: Biomedical Engineering; Biomedical Research; Drug Delivery; Drug Targeting; Hyaluronan; L-tyrosine Polyphosphate; Nanoparticles; Resveratrol; MOMIPP

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Shah, K. (2014). Receptor-Mediated Targeting to Enhance Therapeutic Efficacy of Chemotherapeutic Agents. (Doctoral Dissertation). University of Akron. Retrieved from http://rave.ohiolink.edu/etdc/view?acc_num=akron1390926627

Chicago Manual of Style (16^th Edition):

Shah, Kush. “Receptor-Mediated Targeting to Enhance Therapeutic Efficacy of Chemotherapeutic Agents.” 2014. Doctoral Dissertation, University of Akron. Accessed January 18, 2021. http://rave.ohiolink.edu/etdc/view?acc_num=akron1390926627.

MLA Handbook (7^th Edition):

Shah, Kush. “Receptor-Mediated Targeting to Enhance Therapeutic Efficacy of Chemotherapeutic Agents.” 2014. Web. 18 Jan 2021.

Vancouver:

Shah K. Receptor-Mediated Targeting to Enhance Therapeutic Efficacy of Chemotherapeutic Agents. [Internet] [Doctoral dissertation]. University of Akron; 2014. [cited 2021 Jan 18]. Available from: http://rave.ohiolink.edu/etdc/view?acc_num=akron1390926627.

Council of Science Editors:

Shah K. Receptor-Mediated Targeting to Enhance Therapeutic Efficacy of Chemotherapeutic Agents. [Doctoral Dissertation]. University of Akron; 2014. Available from: http://rave.ohiolink.edu/etdc/view?acc_num=akron1390926627

University of Akron

7. Qaqish, Walid P. Electrospinning of L-Tyrosine Polyurethane Scaffolds for Gene Delivery.

Degree: MSin Engineering, Biomedical Engineering, 2014, University of Akron

URL: http://rave.ohiolink.edu/etdc/view?acc_num=akron1416997407

► Pseudo-poly(amino acids), such as to L-tyrosine polyurethanes (LTUs), are a class of polymers developed to enhance their biocompatibility while offering the ability to tailor mechanical… (more)

▼ Pseudo-poly(amino acids), such as to L-tyrosine polyurethanes (LTUs), are a class of polymers developed to enhance their biocompatibility while offering the ability to tailor mechanical properties. LTU4a, a variant LTU, has been synthesized due to the lack of 1250 Da PCL-marcodiol. LTU4a has been dissolved using a co-solvent of chloroform and hexafluoro-2-propanol (HFIP) and emulsified with aqueous buffer that contained DNA.1 Using a novel in situ stirring device, the emulsion has been maintained throughout the electrospinning process. This method resulted in repeatable `dome’ structures (20-200µm in diameter) within the scaffolds. By varying the speed of the in situ stirring device, the `dome’ diameter size can be controlled and is inversely proportional to the stirring speed. Additionally, degradation products of LTU4a scaffolds exhibits limited toxicity based on the LIVE/DEAD® assay of human dermal fibroblasts (HDF) (>95% viability). The durability of LTU4a scaffolds is shown with limited enzymatic (a-chymotrypsin) degradation (<6%). Furthermore, permeability of emulsion electrospun LTU4a scaffolds decreases as compared to LTU4a scaffolds that only contained fibers using conventional electrospinning methods. Since these domes are hollow and are able to encapsulate aqueous droplets, the in situ stirring device can be used to incorporate bioactive molecules such as plasmid DNA (pDNA). Based on the desirable properties of this electrospinning method, studies has been performed to evaluate the bioactivity of pDNA and polyplexes formed by complexing pDNA with linear polyethylenimine (LPEI). The release of pDNA and polyplexes has been verified via PicoGreen® and gel electrophoresis. However, the released pDNA show damage by restriction enzymatic assays and spectral analysis of the pDNA. The decreased pDNA bioactivity hindered transfection in HDFs. Overall, electrospun LTU4a scaffolds have shown excellent biocompatibility as a tissue engineering platform. Finally, emulsion electrospinning of LTU4a with the in situ stirring device offers new scaffold morphologies, and an easy method to increase the therapeutics used electrospun scaffolds for drug delivery applications Advisors/Committee Members: Yun, Yang (Advisor).

Subjects/Keywords: Biomedical Engineering; Biomedical Research; Polymers; L-Tyrosine, Polyurethanes, Electrospinning, Emulsion, Drug Delivery, DNA

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Qaqish, W. P. (2014). Electrospinning of L-Tyrosine Polyurethane Scaffolds for Gene Delivery. (Masters Thesis). University of Akron. Retrieved from http://rave.ohiolink.edu/etdc/view?acc_num=akron1416997407

Chicago Manual of Style (16^th Edition):

Qaqish, Walid P. “Electrospinning of L-Tyrosine Polyurethane Scaffolds for Gene Delivery.” 2014. Masters Thesis, University of Akron. Accessed January 18, 2021. http://rave.ohiolink.edu/etdc/view?acc_num=akron1416997407.

MLA Handbook (7^th Edition):

Qaqish, Walid P. “Electrospinning of L-Tyrosine Polyurethane Scaffolds for Gene Delivery.” 2014. Web. 18 Jan 2021.

Vancouver:

Qaqish WP. Electrospinning of L-Tyrosine Polyurethane Scaffolds for Gene Delivery. [Internet] [Masters thesis]. University of Akron; 2014. [cited 2021 Jan 18]. Available from: http://rave.ohiolink.edu/etdc/view?acc_num=akron1416997407.

Council of Science Editors:

Qaqish WP. Electrospinning of L-Tyrosine Polyurethane Scaffolds for Gene Delivery. [Masters Thesis]. University of Akron; 2014. Available from: http://rave.ohiolink.edu/etdc/view?acc_num=akron1416997407

University of Georgia

8. Do, Quang Trong. Design, synthesis and evaluation of inhibitors for tryptophan indole-lyase, tyrosine phenol-lyase and enzymatic synthesis of tryptophan from glycerol using tryptophan indole-lyase.

Degree: 2014, University of Georgia

URL: http://hdl.handle.net/10724/28714

► This dissertation is written in chapter format following the guidelines of the Graduate School of The University of Georgia. Chapter 1 will serve as an… (more)

Subjects/Keywords: Tryptophan indole-lyase; tyrosine phenol-lyase; inhibitors; steady-state kinetics; pre-steady-state kinetics; glycerol surplus; Escherichia coli BL21 (DE3); plasmids; L-homotryptophan; L-bishomotryptophan; L-homotyrosine; L-bishomotyrosine.

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Do, Q. T. (2014). Design, synthesis and evaluation of inhibitors for tryptophan indole-lyase, tyrosine phenol-lyase and enzymatic synthesis of tryptophan from glycerol using tryptophan indole-lyase. (Thesis). University of Georgia. Retrieved from http://hdl.handle.net/10724/28714

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Do, Quang Trong. “Design, synthesis and evaluation of inhibitors for tryptophan indole-lyase, tyrosine phenol-lyase and enzymatic synthesis of tryptophan from glycerol using tryptophan indole-lyase.” 2014. Thesis, University of Georgia. Accessed January 18, 2021. http://hdl.handle.net/10724/28714.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Do, Quang Trong. “Design, synthesis and evaluation of inhibitors for tryptophan indole-lyase, tyrosine phenol-lyase and enzymatic synthesis of tryptophan from glycerol using tryptophan indole-lyase.” 2014. Web. 18 Jan 2021.

Vancouver:

Do QT. Design, synthesis and evaluation of inhibitors for tryptophan indole-lyase, tyrosine phenol-lyase and enzymatic synthesis of tryptophan from glycerol using tryptophan indole-lyase. [Internet] [Thesis]. University of Georgia; 2014. [cited 2021 Jan 18]. Available from: http://hdl.handle.net/10724/28714.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Do QT. Design, synthesis and evaluation of inhibitors for tryptophan indole-lyase, tyrosine phenol-lyase and enzymatic synthesis of tryptophan from glycerol using tryptophan indole-lyase. [Thesis]. University of Georgia; 2014. Available from: http://hdl.handle.net/10724/28714

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Texas Medical Center

9. Khong, Hiep. PEPTIDE VACCINE FORMULATION CONTROLS THE DURATION OF ANTIGEN PRESENTATION AND MAGNITUDE OF TUMOR‐SPECIFIC CD8+ T CELL RESPONSE.

Degree: PhD, 2017, Texas Medical Center

URL: https://digitalcommons.library.tmc.edu/utgsbs_dissertations/773

► Despite remarkable progresses in vaccinology, therapeutic cancer vaccines have not achieved their full potential. We previously showed that the duration of antigen presentation critically… (more)

Subjects/Keywords: adjuvant; CD8 T cell; cancer; vaccine; pmel-1; B16 melanoma; L-tyrosine; Immunology and Infectious Disease; Medicine and Health Sciences

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Khong, H. (2017). PEPTIDE VACCINE FORMULATION CONTROLS THE DURATION OF ANTIGEN PRESENTATION AND MAGNITUDE OF TUMOR‐SPECIFIC CD8+ T CELL RESPONSE. (Doctoral Dissertation). Texas Medical Center. Retrieved from https://digitalcommons.library.tmc.edu/utgsbs_dissertations/773

Chicago Manual of Style (16^th Edition):

Khong, Hiep. “PEPTIDE VACCINE FORMULATION CONTROLS THE DURATION OF ANTIGEN PRESENTATION AND MAGNITUDE OF TUMOR‐SPECIFIC CD8+ T CELL RESPONSE.” 2017. Doctoral Dissertation, Texas Medical Center. Accessed January 18, 2021. https://digitalcommons.library.tmc.edu/utgsbs_dissertations/773.

MLA Handbook (7^th Edition):

Khong, Hiep. “PEPTIDE VACCINE FORMULATION CONTROLS THE DURATION OF ANTIGEN PRESENTATION AND MAGNITUDE OF TUMOR‐SPECIFIC CD8+ T CELL RESPONSE.” 2017. Web. 18 Jan 2021.

Vancouver:

Khong H. PEPTIDE VACCINE FORMULATION CONTROLS THE DURATION OF ANTIGEN PRESENTATION AND MAGNITUDE OF TUMOR‐SPECIFIC CD8+ T CELL RESPONSE. [Internet] [Doctoral dissertation]. Texas Medical Center; 2017. [cited 2021 Jan 18]. Available from: https://digitalcommons.library.tmc.edu/utgsbs_dissertations/773.

Council of Science Editors:

Khong H. PEPTIDE VACCINE FORMULATION CONTROLS THE DURATION OF ANTIGEN PRESENTATION AND MAGNITUDE OF TUMOR‐SPECIFIC CD8+ T CELL RESPONSE. [Doctoral Dissertation]. Texas Medical Center; 2017. Available from: https://digitalcommons.library.tmc.edu/utgsbs_dissertations/773

10. Ear, Alexandre. Etude biosynthétique des dérivés polykétides PKS-NRPS de type pyrrocidine chez Acremonium zeae : Biosynthetic study of pyrrocidine related compounds, polyketides PKS-NRPS in Acremonium zeae.

Degree: Docteur es, Chimie bio-Organique, 2014, Université Pierre et Marie Curie – Paris VI

URL: http://www.theses.fr/2014PA066411

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Les composés de type pyrrocidine sont des polykétides PKS-NRPS possédant des activités biologiques intéressantes comme antifongique ou antibiotique. La synthèse totale de ces composés est… (more)

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Les composés de type pyrrocidine sont des polykétides PKS-NRPS possédant des activités biologiques intéressantes comme antifongique ou antibiotique. La synthèse totale de ces composés est un réél challenge comme il est constitué de 10 centres chiraux et d'un macrocycle complexe. L'étude de leur biosynthèse pourrait être d'une aide importante afin de comprendre le mécanisme de formation de cette structure spéciale, et en particulier l'étape de la cyclisation complexe.Le précurseur linéaire de ces polykétides étant composé par une chaine nonakétide (partie PKS) et d'une L-tyrosine (partie NRPS), des hypothèses sur leur biosynthèse ont été émises dans cette thèse. Des expériences d'incorporation de précurseurs marqués ou non vont être réalisées dans différents milieux de culture en vue d'obtenir des informations sur cette biosynthèse, et plus précisément le passage du précurseur linéaire vers la structure polycyclique complexe. En parallèle, des supplémentations des cultures d'A. zeae avec des dérivés de la tyrosine seront faites dans le but d'obtenir des analogues pouvant avoir des activités biologiques nouvelles ou meilleures que les pyrrocidines.

Pyrrocidine and its related compounds are PKS-NRPS polyketides having biological interests such as antifungal or antibiotic activities. The total synthesis of these entities has been challenging since the family of hirsutellone is composed by 10 chiral centers and a complex macrocycle. Studying the biosynthesis of these compounds can be an asset for the comprehension of this special molecular structure, especially for the complex cyclization step. Knowing that the linear precursor of these molecules is constituted by a nonaketide chain (PKS part) and by an L-tyrosine (NRPS part), hypotheses about the biosynthesis of hirsutellone-related compounds have been developed in this thesis. Some incorporation experiments of labeled or unlabeled compounds has been done in different culture media in order to have more information about this biosynthesis, in particular the conversion of the linear precursor into this complex polycyclic structure. In parallel, the supplementation of L-tyrosine derivatives will help us to get some analogs of pyrrocidine which can have new or better activities than natural products.

Advisors/Committee Members: Buisson, Didier (thesis director), Nay, Bastien (thesis director).

Subjects/Keywords: Biosynthèse; Pyrrocidine; PKS-NRPS; L-Tyrosine doublement marquée; Polykétide fongique; Marquage isotopique; Poliketides PKS-NRPS; Pyrrocidine; 547

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Ear, A. (2014). Etude biosynthétique des dérivés polykétides PKS-NRPS de type pyrrocidine chez Acremonium zeae : Biosynthetic study of pyrrocidine related compounds, polyketides PKS-NRPS in Acremonium zeae. (Doctoral Dissertation). Université Pierre et Marie Curie – Paris VI. Retrieved from http://www.theses.fr/2014PA066411

Chicago Manual of Style (16^th Edition):

Ear, Alexandre. “Etude biosynthétique des dérivés polykétides PKS-NRPS de type pyrrocidine chez Acremonium zeae : Biosynthetic study of pyrrocidine related compounds, polyketides PKS-NRPS in Acremonium zeae.” 2014. Doctoral Dissertation, Université Pierre et Marie Curie – Paris VI. Accessed January 18, 2021. http://www.theses.fr/2014PA066411.

MLA Handbook (7^th Edition):

Ear, Alexandre. “Etude biosynthétique des dérivés polykétides PKS-NRPS de type pyrrocidine chez Acremonium zeae : Biosynthetic study of pyrrocidine related compounds, polyketides PKS-NRPS in Acremonium zeae.” 2014. Web. 18 Jan 2021.

Vancouver:

Ear A. Etude biosynthétique des dérivés polykétides PKS-NRPS de type pyrrocidine chez Acremonium zeae : Biosynthetic study of pyrrocidine related compounds, polyketides PKS-NRPS in Acremonium zeae. [Internet] [Doctoral dissertation]. Université Pierre et Marie Curie – Paris VI; 2014. [cited 2021 Jan 18]. Available from: http://www.theses.fr/2014PA066411.

Council of Science Editors:

Ear A. Etude biosynthétique des dérivés polykétides PKS-NRPS de type pyrrocidine chez Acremonium zeae : Biosynthetic study of pyrrocidine related compounds, polyketides PKS-NRPS in Acremonium zeae. [Doctoral Dissertation]. Université Pierre et Marie Curie – Paris VI; 2014. Available from: http://www.theses.fr/2014PA066411

11. Comte, Alexia. Etude de la production d'un antioxydant le 3,4-DHPA par Sulfolobus solfataricus, archée hyperthermophile par des approches multidisciplinaires. : Devouts persons and dedicaters : integration of the elites in the ciuitas of the Allobroges during the High-Empire.

Degree: Docteur es, Microbiologie, 2013, Aix Marseille Université

URL: http://www.theses.fr/2013AIXM4731

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L'objectif est de produire un antioxydant puissant, l'acide 3,4-dihydroxyphénylacétique (3,4-DHPA) à partir de la L-tyrosine chez l'archée hyperthermophile et acidophile, Sulfolobus solfataricus 98/2. Les microorganismes… (more)

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L'objectif est de produire un antioxydant puissant, l'acide 3,4-dihydroxyphénylacétique (3,4-DHPA) à partir de la L-tyrosine chez l'archée hyperthermophile et acidophile, Sulfolobus solfataricus 98/2. Les microorganismes extrêmophiles possèdent des enzymes particulièrement résistantes et intéressantes pour l'industrie.Des cultures ont donc été réalisées en fermenteur contrôlé dans 4 conditions : (i) en présence de glucose avec ou sans L-tyrosine, (ii) en présence de phénol avec ou sans L-tyrosine. Il a été montré que le 3,4-DHPA est synthétisé seulement en présence de phénol et de L-tyrosine. Les gènes codant pour les enzymes impliquées dans cette voie métabolique et potentiellement responsables de la synthèse du 3,4-DHPA ont été identifiés par homologie de séquence chez cette archée.Des études transcriptomiques et protéomiques ont donc été initiées pour confirmer ces hypothèses et tenter de caractériser les enzymes impliquées dans ces voies métaboliques. Plusieurs toluène-4-monooxygénases (T4MO) et une catéchol 2,3-dioxygénase, impliquées dans le métabolisme du phénol et potentiellement dans la voie de dégradation de la L-tyrosine ont été identifiées. Leur production est soumise à une régulation transcriptionnelle dépendant de la présence de phénol. L'analyse des régions génomiques correspondantes a permis de mettre en évidence une région consensus qui pourrait être impliquée dans la fixation d'un facteur de transcription lors de la régulation par le phénol. Ces différentes études ont permis, de déterminer d'une part dans quelles conditions le 3,4-DHPA est synthétisé, d'autre part d'identifier les enzymes qui interviendraient dans le métabolisme de la L–tyrosine.

The aim is to produce a powerful antioxidant, 3,4-dihydroxyphenylacetic acid (3,4-DHPA) from L-tyrosine in the hyperthermophilic and acidophilus archaea, Sulfolobus solfataricus 98/2. Extremophiles microorganisms have resistant enzymes and interesting for industry. Cultures have been carried out in controlled bioreactor four conditions: (i) in the presence of glucose with or without L-tyrosine, (ii) in the presence of phenol with or without L-tyrosine. It has been shown that 3,4-DHPA is synthesized only in the presence of phenol and L-tyrosine. The genes encoding enzymes involved in the metabolic and potentially responsible for the synthesis of 3,4-DHPA pathway have been identified by sequence homology in S. solfataricus.Des transcriptomic and proteomic studies have therefore been initiated to confirm these hypothesis and attempt to characterize the enzymes involved in these pathways. Several toluene-4-monooxygenase (T4MO) and catechol 2,3-dioxygenase involved in the metabolism of phenol and potentially in the degradation pathway of L-tyrosine were identified. Their production is subjected to a dependent transcriptional regulation of the presence of phenol. The analysis of the corresponding genomic regions has highlighted a consensus region that could be involved in the binding of a transcription factor in the regulation of phenol. These studies helped…

Advisors/Committee Members: Erauso, Gaël (thesis director), Lorquin, Jean (thesis director).

Subjects/Keywords: L-tyrosine; Acide 3,4-dihydroxyphénylacétique; Acide 4-hydroxyphénylacétique; Sulfolobus solfataricus 98/2; Toluène -4-monooxygénases; Catéchol 2,3dioxygénase; L-tyrosine; 3,4-dihydroxyphenylacetic acid; 4-hydroxyphenylacetic acid; Sulfolobus solfataricus 98/2; Toluene -4-monooxygenases; Catechol 2,3dioxygenase; 579

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Comte, A. (2013). Etude de la production d'un antioxydant le 3,4-DHPA par Sulfolobus solfataricus, archée hyperthermophile par des approches multidisciplinaires. : Devouts persons and dedicaters : integration of the elites in the ciuitas of the Allobroges during the High-Empire. (Doctoral Dissertation). Aix Marseille Université. Retrieved from http://www.theses.fr/2013AIXM4731

Chicago Manual of Style (16^th Edition):

Comte, Alexia. “Etude de la production d'un antioxydant le 3,4-DHPA par Sulfolobus solfataricus, archée hyperthermophile par des approches multidisciplinaires. : Devouts persons and dedicaters : integration of the elites in the ciuitas of the Allobroges during the High-Empire.” 2013. Doctoral Dissertation, Aix Marseille Université. Accessed January 18, 2021. http://www.theses.fr/2013AIXM4731.

MLA Handbook (7^th Edition):

Comte, Alexia. “Etude de la production d'un antioxydant le 3,4-DHPA par Sulfolobus solfataricus, archée hyperthermophile par des approches multidisciplinaires. : Devouts persons and dedicaters : integration of the elites in the ciuitas of the Allobroges during the High-Empire.” 2013. Web. 18 Jan 2021.

Vancouver:

Comte A. Etude de la production d'un antioxydant le 3,4-DHPA par Sulfolobus solfataricus, archée hyperthermophile par des approches multidisciplinaires. : Devouts persons and dedicaters : integration of the elites in the ciuitas of the Allobroges during the High-Empire. [Internet] [Doctoral dissertation]. Aix Marseille Université 2013. [cited 2021 Jan 18]. Available from: http://www.theses.fr/2013AIXM4731.

Council of Science Editors:

Comte A. Etude de la production d'un antioxydant le 3,4-DHPA par Sulfolobus solfataricus, archée hyperthermophile par des approches multidisciplinaires. : Devouts persons and dedicaters : integration of the elites in the ciuitas of the Allobroges during the High-Empire. [Doctoral Dissertation]. Aix Marseille Université 2013. Available from: http://www.theses.fr/2013AIXM4731

Virginia Tech

12. Shelton, Thomas Earl. Small Phosphomonoesters as Probes of Protein-Tyrosine Phosphatase Active Sites.

Degree: MS, Biochemistry and Anaerobic Microbiology, 1999, Virginia Tech

URL: http://hdl.handle.net/10919/44898

► I evaluated the potential of isomers of the low molecular weight phosphomonoester naphthyl phosphate as general diagnostic substrates for differentiating between two families of protein… (more)

▼ I evaluated the potential of isomers of the low molecular weight phosphomonoester naphthyl phosphate as general diagnostic substrates for differentiating between two families of protein phosphatases: the protein-tyrosine phosphatases [PTPs] and the dual-specificity protein phosphatases [DSPs]. Three PTPs, PTP-1B, Tc-PTPa, and PTP-H1, and three DSPs, Cdc-14, VHR, and IphP, were challenged in vitro with alpha-naphthyl phosphate and beta-naphthyl phosphate. Both the DSPs and PTPs readily hydrolyzed beta-naphthyl phosphate. As expected, the DSPs also hydrolyzed alpha-naphthyl phosphate at rates comparable to beta-naphthyl phosphate and two of the PTPs, PTP-1B and Tc-PTPa, hydrolyzed alpha-naphthyl phosphate at a rate one-tenth that of beta-naphthyl phosphate. However, PTP-H1 hydrolyzed both alpha- and beta- naphthyl phosphate at nearly equal rates. Intriguingly, when challenged with radiolabeled phosphoproteins, PTP-H1 was markedly less stringent, by a factor of 40- to 200- fold, than PTP-1B or Tc-PTPa in its selectivity for [32P]phosphotyrosyl- over [32P]phosphoseryl- proteins in vitro. The DSPs and PTPs listed above also were challenged in vitro with free phosphoserine. Each displayed little or no activity towards free phosphoserine. However, the addition of a hydrophobic "handle" to form N-(cyclohexane carboxyl)-O-phospho-L-serine produced a derivative that was hydrolyzed by IphP at rates comparable to that of the avid substrates p-nitrophenyl phosphate and beta-naphthyl phosphate. VHR also hydrolyzed N-(cyclohexane carboxyl)-O-phospho-L-serine, though at a lower rate than IphP. Cdc14 displayed little activity towards N-(cyclohexane carboxyl)-O-phospho-L-serine. The active site of VHR was mapped and amino acid residues potentially involved in binding N-(cyclohexane carboxyl)-O-phospho-L-serine were identified. The amino acid sequence of VHR was aligned with the amino acid sequences of IphP and Cdc14 to identify the nature of the corresponding residues in IphP and Cdcd14. Low molecular weight phosphomonoesters have proven to be effective in vitro indicators of protein phosphatase activity. They also have shown potential as diagnostic substrates for specific subclasses of protein phosphatases. However, neither alpha- and beta- naphthyl phosphate nor N-(cyclohexane carboxyl)-O-phospho-L-serine proved to be universal discriminatory substrates for the functional subgroups within the family of protein-tyrosine phosphatases. Indeed, the probability of identifying such a substrate would appear to be relatively low. Advisors/Committee Members: Kennelly, Peter J. (committeechair), Potts, Malcolm (committee member), Keenan, Thomas W. (committee member).

Subjects/Keywords: Protein-Tyrosine Phosphatase; N-Benzoyl-O-Phospho-L-Serine; Cyanobacteria; Naphthyl Phosphate

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Shelton, T. E. (1999). Small Phosphomonoesters as Probes of Protein-Tyrosine Phosphatase Active Sites. (Masters Thesis). Virginia Tech. Retrieved from http://hdl.handle.net/10919/44898

Chicago Manual of Style (16^th Edition):

Shelton, Thomas Earl. “Small Phosphomonoesters as Probes of Protein-Tyrosine Phosphatase Active Sites.” 1999. Masters Thesis, Virginia Tech. Accessed January 18, 2021. http://hdl.handle.net/10919/44898.

MLA Handbook (7^th Edition):

Shelton, Thomas Earl. “Small Phosphomonoesters as Probes of Protein-Tyrosine Phosphatase Active Sites.” 1999. Web. 18 Jan 2021.

Vancouver:

Shelton TE. Small Phosphomonoesters as Probes of Protein-Tyrosine Phosphatase Active Sites. [Internet] [Masters thesis]. Virginia Tech; 1999. [cited 2021 Jan 18]. Available from: http://hdl.handle.net/10919/44898.

Council of Science Editors:

Shelton TE. Small Phosphomonoesters as Probes of Protein-Tyrosine Phosphatase Active Sites. [Masters Thesis]. Virginia Tech; 1999. Available from: http://hdl.handle.net/10919/44898

University of Akron

13. Sarkar, Debanjan. DEVELOPMENT AND CHARACTERIZATION OF L-TYROSINE BASED POLYURETHANES FOR TISSUE ENGINEERING APPLICATIONS.

Degree: PhD, Chemical Engineering, 2007, University of Akron

URL: http://rave.ohiolink.edu/etdc/view?acc_num=akron1183991645

► Natural amino acid based synthetic polymers have limited applicability as biomaterial due to several unfavorable material and engineering properties. This has led to the development… (more)

▼ Natural amino acid based synthetic polymers have limited applicability as biomaterial due to several unfavorable material and engineering properties. This has led to the development of a new class of polymers known as ‘pseudo poly(amino acid)s’. Several L-tyrosine based pseudo poly(amino acid)s have been developed and characterized extensively for biomaterial applications. Desaminotyrosine tyrosyl hexyl ester (DTH), a diphenolic dipeptide molecule developed from L-tyrosine and its metabolite, is used to synthesize amino acid based pseudo polymers with improved physical and chemical properties. Polyurethanes are extensively used as biomaterials due to excellent biocompatibility and the ability to tune the structure for a wide range of properties. The uses of polyurethanes are mainly focused on biostable implants and biomedical devices. But polyurethanes have shown their susceptibility to degradation under the conditions of their performance. The use of polyurethanes for tissue engineering applications emerged mainly due to the degradability of the polyurethanes. Biodegradable polyurethanes with degradable linkages are developed by altering their structure and composition. The aim of the research presented in this dissertation is focused on developing L-tyrosine based polyurethanes for biomaterial applications including tissue engineering. L-tyrosine based polyurethanes can be developed by using DTH as the chain extender with different polyols and diisocyantes. The use of amino acid based component will improve the biocompatibility and biodegradability of the polymers for tissue engineering application. In addition, by using the different components, the structure and composition of the polyurethanes can be altered to achieve a range of properties that are pertinent to biomaterial applications. This research describes the design, synthesis and characterization of L-tyrosine based polyurethanes with DTH as the chain extender. The polyurethanes are extensively characterized for different bioengineering properties, including surface characteristics, water absorption, degradation characteristics, and controlled release along with other important chemical, physical, thermal and mechanical characterizations. The structure-property relationships of the polyurethanes were investigated by developing a library of polyurethanes with different polyol and diisocyante. This library provides an important tool to design polyurethanes with relevant properties for biomaterial application. The effect of structure and composition of these polyurethanes in determining the material properties were studied in detail. In addition, blends of the polyurethanes were studied as an alternative to adjust different properties according to the requirements. The results show that L-tyrosine based polyurethanes are potential candidates for biomaterial applications including tissue engineering. The material characteristics are strongly dependent on the polyurethane structure and composition, and therefore a wide range of properties can be achieved by… Advisors/Committee Members: Lopina, Stephanie (Advisor).

Subjects/Keywords: Engineering, Chemical; L-tyrosine; Polyurethane; structure; property; tissue engineering; biomaterial

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Sarkar, D. (2007). DEVELOPMENT AND CHARACTERIZATION OF L-TYROSINE BASED POLYURETHANES FOR TISSUE ENGINEERING APPLICATIONS. (Doctoral Dissertation). University of Akron. Retrieved from http://rave.ohiolink.edu/etdc/view?acc_num=akron1183991645

Chicago Manual of Style (16^th Edition):

Sarkar, Debanjan. “DEVELOPMENT AND CHARACTERIZATION OF L-TYROSINE BASED POLYURETHANES FOR TISSUE ENGINEERING APPLICATIONS.” 2007. Doctoral Dissertation, University of Akron. Accessed January 18, 2021. http://rave.ohiolink.edu/etdc/view?acc_num=akron1183991645.

MLA Handbook (7^th Edition):

Sarkar, Debanjan. “DEVELOPMENT AND CHARACTERIZATION OF L-TYROSINE BASED POLYURETHANES FOR TISSUE ENGINEERING APPLICATIONS.” 2007. Web. 18 Jan 2021.

Vancouver:

Sarkar D. DEVELOPMENT AND CHARACTERIZATION OF L-TYROSINE BASED POLYURETHANES FOR TISSUE ENGINEERING APPLICATIONS. [Internet] [Doctoral dissertation]. University of Akron; 2007. [cited 2021 Jan 18]. Available from: http://rave.ohiolink.edu/etdc/view?acc_num=akron1183991645.

Council of Science Editors:

Sarkar D. DEVELOPMENT AND CHARACTERIZATION OF L-TYROSINE BASED POLYURETHANES FOR TISSUE ENGINEERING APPLICATIONS. [Doctoral Dissertation]. University of Akron; 2007. Available from: http://rave.ohiolink.edu/etdc/view?acc_num=akron1183991645

Indian Institute of Science

14. Goswami, Tridib Kumar. Studies On The Photocytotoxic Effect Of Ferrocene-Conjugated Copper(II) Complexes.

Degree: PhD, Faculty of Science, 2016, Indian Institute of Science

URL: http://etd.iisc.ac.in/handle/2005/2528

► The present thesis deals with different aspects of the chemistry and photo-biology of various ferrocene-conjugated metal complexes, their interaction with double helical DNA, DNA photocleavage… (more)

▼ The present thesis deals with different aspects of the chemistry and photo-biology of various ferrocene-conjugated metal complexes, their interaction with double helical DNA, DNA photocleavage and photo-enhanced cytotoxicity in visible light. Phenyl analogues of the active complexes have been synthesized and used for comparison in biological assays. Chapter I provides an introduction to the potential of metal complexes as photochemotherapeutic agents with special reference to organometallic compounds. A brief overview of Photodynamic Therapy (PDT) as a new modality of cancer treatment has been given. Various modes of non-covalent interactions of small molecules with duplex DNA are mentioned. Recent reports on the metal-based photocytotoxic and DNA cleaving agents including photoactivatable organometallic compounds are discussed. The objective of the present investigation is also presented in this chapter. Chapter II presents the synthesis, characterization, structure, DNA binding, DNA photocleavage, photocytotoxicity, mechanism of cell death and cellular localization of ferrocene-conjugated L-methionine reduced Schiff base Cu(II) complexes of phenanthroline bases. To explore the role of the ferrocenyl moiety the phenyl analogues of the ferrocenyl complexes are synthesized and used as controls for comparison purpose. Chapter III deals with the photo-induced DNA cleavage and photo-enhanced cytotoxicity of ferrocene-appended L-tryptophan Cu(II) complexes of heterocyclic bases. The synthesis, characterization, structural comparisons, DNA binding, DNA photocleavage, photocytotoxic activity and cell death mechanism in visible light are discussed in detail. Chapter IV describes the synthesis, characterization and structure of ferrocenylmethyl-L-tyrosine Cu(II) complexes of phenanthroline bases. The complexes are evaluated for DNA binding, DNA photocleavage and photocytotoxic activity in visible light. The cellular localization of the complexes and the mechanism of cell death induced by the complexes are also discussed. Chapter V presents the photocytotoxic effect of ferrocene-conjugated L-amino acid reduced Schiff base Cu(II) complexes of anthracenyl/pyrenyl imidazophenanthroline. The ability of the complexes to bind to double helical DNA and cleave it under photo-illumination conditions is described. Evaluation of the complexes as photochemotherapeutic agents and comparison with currently clinically available drug Photofrin are presented. The mechanism of cancer cell death and cellular localization of the complexes are studied by fluorescence microscopy. Chapter VI describes the synthesis, characterization and photochemotherapeutic efficacy of Cu(II) complexes having ferrocene-appended L-amino acid reduced Schiff base ligands and the naturally occurring polyphenol curcumin. Stabilization of curcumin by complexation to metal for improved photodynamic effect in cancer cells is described with comparison to the parent dye and clinically used drug Photofrin. The mechanism of cell death induced by the copper… Advisors/Committee Members: Chakravarty, Akhil R (advisor).

Subjects/Keywords: Ferrocene Chemistry - Photochemotherapeutic Application; Ferrocene Conjugated Copper(II) Complexes; Photoactivated Metallopharmaceuticals; Ferrocene Conjugates; Ferrocene Conjugated Copper Complexes - DNA Binding; Metal Complexes - Photochemotherapeutic Agents; Organometallic Compounds - Photochemotherapeutic Agents; Photodynamic Therapy; Phenanthroline Bases; Ferrocene-Conjugated L-Tryptophan Copper(II) Complexes; DNA Photocleavage; Photocytotoxicity; Ferrocenylmethyl-L-tyrosine Cu(II) Complexes; Photoinduced DNA; Cellular Localization; Inorganic Chemistry

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Goswami, T. K. (2016). Studies On The Photocytotoxic Effect Of Ferrocene-Conjugated Copper(II) Complexes. (Doctoral Dissertation). Indian Institute of Science. Retrieved from http://etd.iisc.ac.in/handle/2005/2528

Chicago Manual of Style (16^th Edition):

Goswami, Tridib Kumar. “Studies On The Photocytotoxic Effect Of Ferrocene-Conjugated Copper(II) Complexes.” 2016. Doctoral Dissertation, Indian Institute of Science. Accessed January 18, 2021. http://etd.iisc.ac.in/handle/2005/2528.

MLA Handbook (7^th Edition):

Goswami, Tridib Kumar. “Studies On The Photocytotoxic Effect Of Ferrocene-Conjugated Copper(II) Complexes.” 2016. Web. 18 Jan 2021.

Vancouver:

Goswami TK. Studies On The Photocytotoxic Effect Of Ferrocene-Conjugated Copper(II) Complexes. [Internet] [Doctoral dissertation]. Indian Institute of Science; 2016. [cited 2021 Jan 18]. Available from: http://etd.iisc.ac.in/handle/2005/2528.

Council of Science Editors:

Goswami TK. Studies On The Photocytotoxic Effect Of Ferrocene-Conjugated Copper(II) Complexes. [Doctoral Dissertation]. Indian Institute of Science; 2016. Available from: http://etd.iisc.ac.in/handle/2005/2528

15. 小平, 明果; コダイラ, サヤカ. Advantages of L-3-[18F]-fluoro-alpha-methyl tyrosine over 2-[18F]-fluoro-2-deoxyglucose in detecting liver metastasis during positron emission tomography scan : 転移性肝腫瘍の検出におけるFAMT-PETのFDG-PETに対する優位性について.

Degree: 博士（医学）, 2016, Gunma University / 群馬大学

URL: http://hdl.handle.net/10087/10216

学位記番号：医博甲1593号

Subjects/Keywords: Liver metastasis; L-3-[18F] fluoro-alpha-methyl tyrosine; 2-[18F]-fluoro-2-deoxyglucose; Positron emission tomography

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

小平, 明果; コダイラ, �. (2016). Advantages of L-3-[18F]-fluoro-alpha-methyl tyrosine over 2-[18F]-fluoro-2-deoxyglucose in detecting liver metastasis during positron emission tomography scan : 転移性肝腫瘍の検出におけるFAMT-PETのFDG-PETに対する優位性について. (Thesis). Gunma University / 群馬大学. Retrieved from http://hdl.handle.net/10087/10216

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

小平, 明果; コダイラ, サヤカ. “Advantages of L-3-[18F]-fluoro-alpha-methyl tyrosine over 2-[18F]-fluoro-2-deoxyglucose in detecting liver metastasis during positron emission tomography scan : 転移性肝腫瘍の検出におけるFAMT-PETのFDG-PETに対する優位性について.” 2016. Thesis, Gunma University / 群馬大学. Accessed January 18, 2021. http://hdl.handle.net/10087/10216.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

小平, 明果; コダイラ, サヤカ. “Advantages of L-3-[18F]-fluoro-alpha-methyl tyrosine over 2-[18F]-fluoro-2-deoxyglucose in detecting liver metastasis during positron emission tomography scan : 転移性肝腫瘍の検出におけるFAMT-PETのFDG-PETに対する優位性について.” 2016. Web. 18 Jan 2021.

Vancouver:

小平, 明果; コダイラ �. Advantages of L-3-[18F]-fluoro-alpha-methyl tyrosine over 2-[18F]-fluoro-2-deoxyglucose in detecting liver metastasis during positron emission tomography scan : 転移性肝腫瘍の検出におけるFAMT-PETのFDG-PETに対する優位性について. [Internet] [Thesis]. Gunma University / 群馬大学; 2016. [cited 2021 Jan 18]. Available from: http://hdl.handle.net/10087/10216.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

小平, 明果; コダイラ �. Advantages of L-3-[18F]-fluoro-alpha-methyl tyrosine over 2-[18F]-fluoro-2-deoxyglucose in detecting liver metastasis during positron emission tomography scan : 転移性肝腫瘍の検出におけるFAMT-PETのFDG-PETに対する優位性について. [Thesis]. Gunma University / 群馬大学; 2016. Available from: http://hdl.handle.net/10087/10216

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

16. Daly, Davis. ”Nonlinear optical properties of polymers containing azomesogens and chiral molecules: theoretical and experimental evaluations.".

Degree: Applied Chemistry, 2005, Cochin University of Science and Technology

URL: http://dyuthi.cusat.ac.in/purl/3827

►

The present work emphasizes the use of chirality as an efficient tool to synthesize new types of second order nonlinear materials. Second harmonic generation efficiency… (more)

Subjects/Keywords: NONLINEAR OPTICAL PROPERTIES OF POLYMERS; OPTIMIZATION; SUBSTITUENT POSITION,SPACER GROUPS; CI-IIRAL POLYESTERS; L-TYROSINE

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APA (6^th Edition):

Daly, D. (2005). ”Nonlinear optical properties of polymers containing azomesogens and chiral molecules: theoretical and experimental evaluations.". (Thesis). Cochin University of Science and Technology. Retrieved from http://dyuthi.cusat.ac.in/purl/3827

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Daly, Davis. “”Nonlinear optical properties of polymers containing azomesogens and chiral molecules: theoretical and experimental evaluations.".” 2005. Thesis, Cochin University of Science and Technology. Accessed January 18, 2021. http://dyuthi.cusat.ac.in/purl/3827.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Daly, Davis. “”Nonlinear optical properties of polymers containing azomesogens and chiral molecules: theoretical and experimental evaluations.".” 2005. Web. 18 Jan 2021.

Vancouver:

Daly D. ”Nonlinear optical properties of polymers containing azomesogens and chiral molecules: theoretical and experimental evaluations.". [Internet] [Thesis]. Cochin University of Science and Technology; 2005. [cited 2021 Jan 18]. Available from: http://dyuthi.cusat.ac.in/purl/3827.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Daly D. ”Nonlinear optical properties of polymers containing azomesogens and chiral molecules: theoretical and experimental evaluations.". [Thesis]. Cochin University of Science and Technology; 2005. Available from: http://dyuthi.cusat.ac.in/purl/3827

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

17. Reho, John Joseph. The Impact of Reductions in Uterine Perfusion Pressure on Uterine Arterial Reactivity in Gravid Rats II and L-tyrosine Polyphosphate Nanoparticles as a Potential In Vivo Gene Delivery Device.

Degree: PhD, Integrated Bioscience, 2012, University of Akron

URL: http://rave.ohiolink.edu/etdc/view?acc_num=akron1333899328

► Reductions in uterine perfusion pressure are thought to be a central component of the pathological pregnancy disease, preeclampsia. Preeclampsia is a hypertensive disorder of pregnancy… (more)

▼ Reductions in uterine perfusion pressure are thought to be a central component of the pathological pregnancy disease, preeclampsia. Preeclampsia is a hypertensive disorder of pregnancy characterized by vascular dysfunction and end organ underperfusion and is the leading cause of maternal and fetal morbidity and mortality in the United States and throughout the world. Uterine arterial reactivity and structural mechanics during preeclampsia are poorly understood and likely contributed to the pathophysiology of the maternal hypertension and altered fetal growth demonstrated in this disease. The first aim of this dissertation project was to characterize the impact of reductions in uterine perfusion pressure on maternal and fetal pregnancy outcomes. Maternal hypertension and fetal morphometrics have been examined in response to reductions in uterine perfusion pressure. The second aim of this research was to characterize the vascular behavior and structural biophysical mechanics of resistance-caliber uterine arteries in response to reductions in uterine perfusion pressure. Vascular behavior was examined using a pressurized arteriograph where myogenic reactivity, agonist induced vasodilation and vasoconstriction, and passive structural mechanics were assessed. The third aim of this research involved testing the efficacy of a novel L-tyrosine based gene delivery device. L-tyrosine polyphosphate (LTP) nanoparticles have demonstrated promise as a potential intracellular gene delivery device aimed at therapeutic avenues however; the in vivo efficacy of the delivery vehicle has been unknown. We aimed to prove the concept that LTP nanoparticles encapsulated with plasmid DNA would be efficacious as an in vivo gene delivery device in the rat uterus. Chronic reductions in uterine perfusion pressure resulted in maternal hypertension and severe fetal growth restriction in pregnant rats suggesting an integral role of uterine perfusion pressure on maternal and fetal responses to the pathology. Uterine artery reactivity was found to be altered towards a constrictive phenotype in response to chronic reductions in uterine perfusion pressure with increased myogenic reactivity and decreased agonist induced vasodilation. Structural parameters of resistance-caliber uterine arteries were unaltered in response to the pathology while biophysical mechanical properties of the uterine arteries were altered. Distensibility was altered in isolated resistance-caliber uterine arteries isolated from reductions in uterine perfusion pressure gravid rats suggesting a potential contributing factor to the vascular dysfunction described above. Nanoparticles formulated from L-tyrosine polyphosphate successfully delivered plasmid DNA in vivo to the rat uterine tissue. These data suggest that uterine arterial reactivity is altered in response to reductions in uterine perfusion pressure and that LTP nanoparticles encapsulated with plasmid DNA may have potential as a delivery platform for therapies aimed at diseases of the uterus and uterine vasculature. Advisors/Committee Members: Ramirez, Rolando (Advisor).

Subjects/Keywords: Biomedical Engineering; Uterine artery; preeclampsia; pregnancy; gene delivery; nanoparticles; L-tyrosine polyphosphate

…Biodegradable Particles �� . .......................19 L-tyrosine Polyphosphate Polymer… …20 L-tyrosine Polyphosphate Nanoparticles �� … ..........................22 DNA-LPEI… …FORMULATED FROM L-TYROSINE POLYPHOSPHATE AS A POTENTIAL NON-VIRAL INTRACELLULAR GENE DELIVERY… …11 2.2 Structural of L-tyrosine polyphosphate (LTP) �� .... �� 22 2.3… …delivery device formulated from L-tyrosine based polymers and examines its potential as an in…

13 more images…

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APA (6^th Edition):

Reho, J. J. (2012). The Impact of Reductions in Uterine Perfusion Pressure on Uterine Arterial Reactivity in Gravid Rats II and L-tyrosine Polyphosphate Nanoparticles as a Potential In Vivo Gene Delivery Device. (Doctoral Dissertation). University of Akron. Retrieved from http://rave.ohiolink.edu/etdc/view?acc_num=akron1333899328

Chicago Manual of Style (16^th Edition):

Reho, John Joseph. “The Impact of Reductions in Uterine Perfusion Pressure on Uterine Arterial Reactivity in Gravid Rats II and L-tyrosine Polyphosphate Nanoparticles as a Potential In Vivo Gene Delivery Device.” 2012. Doctoral Dissertation, University of Akron. Accessed January 18, 2021. http://rave.ohiolink.edu/etdc/view?acc_num=akron1333899328.

MLA Handbook (7^th Edition):

Reho, John Joseph. “The Impact of Reductions in Uterine Perfusion Pressure on Uterine Arterial Reactivity in Gravid Rats II and L-tyrosine Polyphosphate Nanoparticles as a Potential In Vivo Gene Delivery Device.” 2012. Web. 18 Jan 2021.

Vancouver:

Reho JJ. The Impact of Reductions in Uterine Perfusion Pressure on Uterine Arterial Reactivity in Gravid Rats II and L-tyrosine Polyphosphate Nanoparticles as a Potential In Vivo Gene Delivery Device. [Internet] [Doctoral dissertation]. University of Akron; 2012. [cited 2021 Jan 18]. Available from: http://rave.ohiolink.edu/etdc/view?acc_num=akron1333899328.

Council of Science Editors:

Reho JJ. The Impact of Reductions in Uterine Perfusion Pressure on Uterine Arterial Reactivity in Gravid Rats II and L-tyrosine Polyphosphate Nanoparticles as a Potential In Vivo Gene Delivery Device. [Doctoral Dissertation]. University of Akron; 2012. Available from: http://rave.ohiolink.edu/etdc/view?acc_num=akron1333899328

University of Akron

18. Shah, Parth Nimish. Biocompatibility Analysis and Biomedical Device Development Using Novel L-Tyrosine Based Polymers.

Degree: PhD, Chemical Engineering, 2009, University of Akron

URL: http://rave.ohiolink.edu/etdc/view?acc_num=akron1238781002

► L-tyrosine based ‘pseudo’ poly (amino acids) such as polyphosphates and polyurethanes have been developed using desaminotyrosine tyrosyl hexyl ester (DTH) as the monomer, and… (more)

▼ L-tyrosine based ‘pseudo’ poly (amino acids) such as polyphosphates and polyurethanes have been developed using desaminotyrosine tyrosyl hexyl ester (DTH) as the monomer, and characterized with the aim of using them for biomedical applications. The successful establishment of the biocompatibility of these novel materials is critical for their success and acceptance in the biomedical device field. One of the main aims of the research presented in this dissertation has been to evaluate the biocompatibility of novel L-tyrosine based polymers and their degradation products by examining their cytotoxicity, investigating the adhesion and proliferation of human fibroblast cells on L-tyrosine based polymeric substrates, and correlating the cell adhesion to surface wettability and composition of the substrates. Characterization results of L-tyrosine based polyurethanes and polyphosphate have shown that although these polymers are synthesized using a common monomer, they exhibit dramatically different physico-mechanical properties. Another aim of this dissertation has been to examine the blending of polyphosphate and polyurethanes in order to develop materials with a wider spectrum of physico-mechanical properties and thus obtain a stepwise transition in the material properties by adjusting the blends composition. The blends have been extensively characterized for different bioengineering properties including surface and bulk characteristics. In addition, the possibility of application of polyphosphate and polyurethanes for the formulation of controlled drug delivery devices has been investigated. Drug delivery devices in the form of microparticles and electrospun micro- and nano-fibrous membranes have been developed and certain process parameters associated with the formulation process have been optimized. The results indicate that L-tyrosine based polymers and their degradation products are non-cytotoxic under test conditions (dosages and time frames examined). The adhesion of cells onto L-tyrosine based polymeric substrates has been found to be a function of the polymer chemistry and its surface wettability. Polymers with moderate hydrophobicity have been found to promote cellular adhesion whereas hydrophilic surfaces retarded cellular adhesion and proliferation. These results coupled with the physico-chemical and mechanical properties of these novel polymers suggest that they are prospective candidates for biomedical applications including tissue engineering and drug delivery devices. Further, results obtained from blend characterizations indicate that the material properties of blends are a strong function of the blend composition and blending of polyphosphate and polyurethanes provides an easy way to obtain materials with a wide range of properties intermediate to the parent polymer properties. Finally, the successful development of drug delivery devices in the form of microparticles and micro- and nano-fibrous membranes has shown that these novel materials can indeed prove to be a very valuable… Advisors/Committee Members: Lopina, Stephanie (Advisor), Yun, Yang (Advisor).

Subjects/Keywords: Biomedical Research; Chemical Engineering; Polymers; Biomaterials; Microparticles; Electrospinning; Biocompatibility; Cytotoxicity; Cell Adhesion; Blends; L-Tyrosine

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APA (6^th Edition):

Shah, P. N. (2009). Biocompatibility Analysis and Biomedical Device Development Using Novel L-Tyrosine Based Polymers. (Doctoral Dissertation). University of Akron. Retrieved from http://rave.ohiolink.edu/etdc/view?acc_num=akron1238781002

Chicago Manual of Style (16^th Edition):

Shah, Parth Nimish. “Biocompatibility Analysis and Biomedical Device Development Using Novel L-Tyrosine Based Polymers.” 2009. Doctoral Dissertation, University of Akron. Accessed January 18, 2021. http://rave.ohiolink.edu/etdc/view?acc_num=akron1238781002.

MLA Handbook (7^th Edition):

Shah, Parth Nimish. “Biocompatibility Analysis and Biomedical Device Development Using Novel L-Tyrosine Based Polymers.” 2009. Web. 18 Jan 2021.

Vancouver:

Shah PN. Biocompatibility Analysis and Biomedical Device Development Using Novel L-Tyrosine Based Polymers. [Internet] [Doctoral dissertation]. University of Akron; 2009. [cited 2021 Jan 18]. Available from: http://rave.ohiolink.edu/etdc/view?acc_num=akron1238781002.

Council of Science Editors:

Shah PN. Biocompatibility Analysis and Biomedical Device Development Using Novel L-Tyrosine Based Polymers. [Doctoral Dissertation]. University of Akron; 2009. Available from: http://rave.ohiolink.edu/etdc/view?acc_num=akron1238781002

University of Akron

19. Ditto, Andrew James. Biodegradable Nanoparticles for Use as an Inhalable Antimicrobial and as a Receptor Targeted Delivery Device.

Degree: PhD, Biomedical Engineering, 2010, University of Akron

URL: http://rave.ohiolink.edu/etdc/view?acc_num=akron1280335303

► Drug delivery challenges include drug instability, insolubility, dosing, toxicity, and targeting, which can be addressed by encapsulation into degradable nanoparticles that can be modified for… (more)

▼ Drug delivery challenges include drug instability, insolubility, dosing, toxicity, and targeting, which can be addressed by encapsulation into degradable nanoparticles that can be modified for numerous applications. Previous studies have demonstrated the synthesis and characterization of degradable nanoparticles formulated form L-tyrosine polyphosphate (LTP) that show promise for a wide array of drug delivery applications. One such application is inhalable antimicrobials to chronically infected lungs of cystic fibrosis patients, which currently suffer from fast clearance, poor stability, and resistance. An additional application is receptor targeted anticancer drug delivery, since current anticancer agents, such as cisplatin, may cause nonspecific toxicity. Therefore, we have produced and characterized nanoparticles formulated primarily from LTP that can be modified for use as an inhalable antimicrobial loaded with silver carbene complexes (SCC), or as a receptor targeted delivery system decorated with folic acid. Dynamic light scattering shows diameter ranges around 1 µm that is optimal for inhalable delivery, or approximately 200 nm that is optimal for anticancer drug endocytosis. SCC’s are release over 7 days in vitro from LTP nanoparticles. Furthermore, degrading LTP nanoparticles do not lower the pH unlike poly(lactic-co-glycolic acid) (PLGA) nanoparticles that lower the pH to acidic levels. This lack of acidic degradation products results in low fibroblast toxicity comparable to PLGA nanoparticles and buffers as shown by MTT and LIVE/DEAD assays. These toxicity assays also reveal mean lethal dosage values less than minimum inhibitory concentrations from clinically relevant in vitro planktonic and biofilm antibacterial assays. This antibacterial efficacy is reflected with an in vivo mouse model, in which SCC-LTP nanoparticles achieve a 100% survival advantage. Furthermore, folic acid decoration of LTP nanoparticles demonstrates a receptor mediated targeting with a 10-fold greater attachment to HeLa cancer cells under simulated physiological flow compared to undecorated nanoparticles and non-cancerous fibroblasts. This attachment is inhibited with excess folic acid in the perfusion solution. A 30 minute incubation and LIVE/DEAD assay show these folic acid decorated nanoparticles loaded with SCC’s have increased anticancer efficacy compared to drug alone. Thus, LTP nanoparticles can be utilized for a wide array of drug delivery applications including inhalable antimicrobials or receptor targeted anticancer drug delivery. Advisors/Committee Members: Yun, Yang (Advisor).

Subjects/Keywords: Biomedical Research; nanoparticles; drug delivery; antimicrobial; targeted; targeting; folic acid; L-tyrosine; degradable; sustained release; silver; SCC; LTP

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APA (6^th Edition):

Ditto, A. J. (2010). Biodegradable Nanoparticles for Use as an Inhalable Antimicrobial and as a Receptor Targeted Delivery Device. (Doctoral Dissertation). University of Akron. Retrieved from http://rave.ohiolink.edu/etdc/view?acc_num=akron1280335303

Chicago Manual of Style (16^th Edition):

Ditto, Andrew James. “Biodegradable Nanoparticles for Use as an Inhalable Antimicrobial and as a Receptor Targeted Delivery Device.” 2010. Doctoral Dissertation, University of Akron. Accessed January 18, 2021. http://rave.ohiolink.edu/etdc/view?acc_num=akron1280335303.

MLA Handbook (7^th Edition):

Ditto, Andrew James. “Biodegradable Nanoparticles for Use as an Inhalable Antimicrobial and as a Receptor Targeted Delivery Device.” 2010. Web. 18 Jan 2021.

Vancouver:

Ditto AJ. Biodegradable Nanoparticles for Use as an Inhalable Antimicrobial and as a Receptor Targeted Delivery Device. [Internet] [Doctoral dissertation]. University of Akron; 2010. [cited 2021 Jan 18]. Available from: http://rave.ohiolink.edu/etdc/view?acc_num=akron1280335303.

Council of Science Editors:

Ditto AJ. Biodegradable Nanoparticles for Use as an Inhalable Antimicrobial and as a Receptor Targeted Delivery Device. [Doctoral Dissertation]. University of Akron; 2010. Available from: http://rave.ohiolink.edu/etdc/view?acc_num=akron1280335303

Indian Institute of Science

20. Roy, Gouriprasanna. Biomimetic Studies On Anti-Thyroid Drugs And Thyroid Hormone Synthesis.

Degree: PhD, Faculty of Science, 2009, Indian Institute of Science

URL: http://etd.iisc.ac.in/handle/2005/514

► Thyroxine (T4), the main secretory hormone of the thyroid gland, is produced on thyroglobulin by thyroid peroxidase (TPO)/hydrogen peroxide/iodide system. The synthesis of T4 by… (more)

▼ Thyroxine (T4), the main secretory hormone of the thyroid gland, is produced on thyroglobulin by thyroid peroxidase (TPO)/hydrogen peroxide/iodide system. The synthesis of T4 by TPO involves two independent steps: iodination of tyrosine and phenolic coupling of the resulting iodotyrosine residues. The prohormone T4 is then converted to its biologically active form T3 by a selenocysteine-containing iodothyronine deiodinase (ID-I), which is present in highest amounts in liver, kidney, thyroid and pituitary. The 5'-deiodination catalyzed by ID-I is a ping-pong, bisubstrate reaction in which the selenol (or selenolate) group of the enzyme (E-SeH or E-Se-) first reacts with thyroxine (T4) to form a selenenyl iodide (E-SeI) intermediate. Subsequent reaction of the selenenyl iodide with an as yet unidentified intracellular cofactor completes the catalytic cycle and regenerates the selenol. Although the deiodination reactions are essential for the function of thyroid gland, the activation of thyroid stimulating hormone (TSH) receptor by auto-antibodies leads to an overproduction of thyroid hormones. In addition, these antibodies stimulate ID-I and probably other deiodinases to produce relatively more amount of T3. Figure 1. Synthesis of thyroid hormones by heme-containing Thyroid Peroxidase(TPO)(Refer PDF File) As these antibodies are not under pituitary feedback control system, there is no negative influence on the thyroid activity and, therefore, the uncontrolled production of thyroid hormones leads to a condition called “hyperthyroidism”. Under these conditions, the overproduction of T4 and T3 can be controlled by specific inhibitors, which either block the thyroid hormone biosynthesis or reduce the conversion of T4 to T3. A unique class of such inhibitors is the thiourea drugs, methimazole (1, MMI), 6-n-propyl-2-thiouracil (3, PTU), and 6-methyl-2-thiouracil (5, MTU). Although these compounds are the most commonly employed drugs in the treatment of hyperthyroidism, the detailed mechanism of their action is still not clear. According to the initially proposed mechanism, these drugs may divert oxidized iodides away from thyroglobulin by forming stable electron donor-acceptor complexes with diiodine, which can effectively reduce the thyroid hormone biosynthesis. It has also been proposed that these drugs may block the thyroid hormone synthesis by coordinating to the metal center of thyroid peroxidase (TPO). After the discovery that the ID-I is responsible for the activation of thyroxine, it has been reported that PTU, but not MMI, reacts with the selenenyl iodide intermediate (E-SeI) of ID-I to form a selenenyl sulfide as a dead end product, thereby blocking the conversion of T4 to T3 during the monodeiodination reaction. The mechanism of anti-thyroid activity is further complicated by the fact that the gold-containing drugs such as gold thioglucose (GTG) inhibit the deiodinase activity by reacting with the selenol group of the native enzyme. Recently, the selenium analogues 2 (MSeI), 4 (PSeU) and 6 (MSeU) attracted… Advisors/Committee Members: Mugesh, G (advisor).

Subjects/Keywords: Anti-Thyroid Drugs; Thyroid Hormone Synthesis; Biomimetics; Thyroid Hormones - Biosynthesis; Se-Methimazole (MSel); L-Tyrosine; Thiones; Selones; Anti-Hyperthyroid Drugs; Thyroid Gland; Thyroxine (T4); Bioinorganic Chemistry

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APA (6^th Edition):

Roy, G. (2009). Biomimetic Studies On Anti-Thyroid Drugs And Thyroid Hormone Synthesis. (Doctoral Dissertation). Indian Institute of Science. Retrieved from http://etd.iisc.ac.in/handle/2005/514

Chicago Manual of Style (16^th Edition):

Roy, Gouriprasanna. “Biomimetic Studies On Anti-Thyroid Drugs And Thyroid Hormone Synthesis.” 2009. Doctoral Dissertation, Indian Institute of Science. Accessed January 18, 2021. http://etd.iisc.ac.in/handle/2005/514.

MLA Handbook (7^th Edition):

Roy, Gouriprasanna. “Biomimetic Studies On Anti-Thyroid Drugs And Thyroid Hormone Synthesis.” 2009. Web. 18 Jan 2021.

Vancouver:

Roy G. Biomimetic Studies On Anti-Thyroid Drugs And Thyroid Hormone Synthesis. [Internet] [Doctoral dissertation]. Indian Institute of Science; 2009. [cited 2021 Jan 18]. Available from: http://etd.iisc.ac.in/handle/2005/514.

Council of Science Editors:

Roy G. Biomimetic Studies On Anti-Thyroid Drugs And Thyroid Hormone Synthesis. [Doctoral Dissertation]. Indian Institute of Science; 2009. Available from: http://etd.iisc.ac.in/handle/2005/514

Freie Universität Berlin

21. Plotkin, Michail. Implicatzions of nuclear medicine imaging using radioactively labelled amino acids in the neurooncology and in the diagnosis of head and neck cancer.

Degree: 2007, Freie Universität Berlin

URL: http://dx.doi.org/10.17169/refubium-8697

► An advantage of the radioactively labelled amino acids in the neurooncological diagnostics lies in their ability to accumulate in nearly all brain tumours. Moreover, the… (more)

Subjects/Keywords: 3-[I-123] iodo- Ñ-methyl-L-tyrosine (IMT)¡V O-(2-[18F]fluoroethyl)-L-tyrosine (FET) ¡Vsingle photon emission tomography (SPECT) ¡V positron emission tomography (PET) ¡Vmagnetic resonance imaging (MRI) ¡V magnetic resonance spectroscopy (MRS) image fusion ¡V glioma ¡V head and neck cancer - recurrence; 600 Technik, Medizin, angewandte Wissenschaften::610 Medizin und Gesundheit::610 Medizin und Gesundheit

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Plotkin, M. (2007). Implicatzions of nuclear medicine imaging using radioactively labelled amino acids in the neurooncology and in the diagnosis of head and neck cancer. (Thesis). Freie Universität Berlin. Retrieved from http://dx.doi.org/10.17169/refubium-8697

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Plotkin, Michail. “Implicatzions of nuclear medicine imaging using radioactively labelled amino acids in the neurooncology and in the diagnosis of head and neck cancer.” 2007. Thesis, Freie Universität Berlin. Accessed January 18, 2021. http://dx.doi.org/10.17169/refubium-8697.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Plotkin, Michail. “Implicatzions of nuclear medicine imaging using radioactively labelled amino acids in the neurooncology and in the diagnosis of head and neck cancer.” 2007. Web. 18 Jan 2021.

Vancouver:

Plotkin M. Implicatzions of nuclear medicine imaging using radioactively labelled amino acids in the neurooncology and in the diagnosis of head and neck cancer. [Internet] [Thesis]. Freie Universität Berlin; 2007. [cited 2021 Jan 18]. Available from: http://dx.doi.org/10.17169/refubium-8697.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Plotkin M. Implicatzions of nuclear medicine imaging using radioactively labelled amino acids in the neurooncology and in the diagnosis of head and neck cancer. [Thesis]. Freie Universität Berlin; 2007. Available from: http://dx.doi.org/10.17169/refubium-8697

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Open Access Theses and Dissertations